LOCUS       DASVRK010000001        34139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_311270, whole genome shotgun sequence.
ACCESSION   DASVRK010000001 DASVRK010000000
VERSION     DASVRK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 34139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34139
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311270"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1270
                     /locus_tag="VF589_00005"
     CDS             161..1270
                     /locus_tag="VF589_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX8255984.1"
                     /translation="MTASPSLSAERPRARQAGIAVGILAPGPLNAITDVAGVVVGHVT
                     LKEGAAIRTGVTAVLPHRGNLFQDKVPAGFAVANGFGKFMGSTQIEELGEIETPILLT
                     NTLSVPEAAAAVVEWTLAQPGNEQVRSVNAVVGETNDGQLNDIRARRVTKADGLAAIA
                     AAREGRVAEGAVGAGTGTVAFGWKGGIGTSSRRLPARLGGWTVGVLVQSNYGGVLTVD
                     GVRVGEALGRYMLKDELEGAAGDGSVIVVVATDAPLSDRNLKRLARRAFLGIARTGSP
                     ITNGSGDYAVAFSTAAAVRRTPARRSAVAGMEELPNDRVTPLFQAAVEATEEAVLNSM
                     FKAETMEANGARVEALPVAEVLQLHRRGSGRARGE"
     gene            1273..1674
                     /locus_tag="VF589_00010"
     CDS             1273..1674
                     /locus_tag="VF589_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8255985.1"
                     /translation="MRALLVLAAFSAMGSQASAIATAGPLTGAWGGEHVGLELDAEGG
                     RVRYDCAAGTIDGPVLPGASGRFQANGAHRPNLGGPERQGREPPLLPAIYTGTVRGDI
                     MTLSVRVPSRAMEIGPFTLRRGAEPILLRCL"
     gene            1771..3633
                     /locus_tag="VF589_00015"
     CDS             1771..3633
                     /locus_tag="VF589_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEX8255986.1"
                     /translation="MRFLTLLPLAALVLTGIAPAPMAAQPGARRPAALDAEAICRPFL
                     DASFGDSDSRGRYGPARALAPPPPPPPPSPQQPGAPAEAATDSEVVVTGRRIAEPNLT
                     AVSPVTVVNSQEVQLRGTVRTQNLMNSGPQASAQAPGSYVRDPANRERYAGKEVAAVQ
                     AVAEAPVSTFAVDVDTGSYANVRRMLNAGEMPPQAAIRTEEMLNYFRYDYSAPRDRSR
                     PFTITTDMTTTPWHPTTRLLRVGLRGYDVARSERPAANLVFLVDVSGSMEEPNKLPLV
                     KCSLALLAERLSPRDRVSIVVYAGAAGRVLEPTSDKRRVLDVLARLRAGGSTAGAQGI
                     QLAYETARAGMIEGGINRVVLATDGDFNVGVTNDQALIDMVERERAAGTTLTTLGYGT
                     GTYNEAMMEQIADHGNGNYAYIDGPREAQKVLDQELASTLFTIAGDVKIQVEFNPAYV
                     SEYRLIGYENRALAEQDFDNDAVDAGDIGAGHQVTALYEIVPAGSRGWLPGRRYPANR
                     REASGMGNDELAWLRLRYKLPGENQSRLIERPVPAGLVRSARAPAGDTAFAAAVAAYS
                     QRLRGDPLLANFSFADARRLAARADTRDPWRREFVGLTEAAERLTSERAGGAGR"
     gene            complement(3637..4302)
                     /locus_tag="VF589_00020"
     CDS             complement(3637..4302)
                     /locus_tag="VF589_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8255987.1"
                     /translation="MIPRLLIDTAQIPGGGELRLYRRAEEFSIMLGGNELMNSRLSGS
                     EEALATLVCERIGGRRDPKLLIGGLGMGFTLRAALAALGPDARIEIAELVPAVIAWAR
                     GPLAPLFGTSLSDPRVELIEADVAGLIDAGRGYDAILLDVDNGPDGIVRDDNERLYQP
                     AGLARAKAALRPGGILAIWSAAPHRNFAQRLRAAGFAVEEVPVRAAGGRGARHVIWLA
                     RRG"
     gene            complement(4326..6848)
                     /gene="gyrB"
                     /locus_tag="VF589_00025"
     CDS             complement(4326..6848)
                     /gene="gyrB"
                     /locus_tag="VF589_00025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX8255988.1"
                     /translation="MASSPTENAYGAESIKVLKGLDAVRKRPGMYIGDTDDGSGLHHM
                     VFEVSDNAIDEALAGHCDLIQITLNPDGSVSVEDNGRGIPTGIHSGEGVSAAEVIMTQ
                     LHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLDLTIWRDGEEHYMRFRHGDAE
                     APLKVVGPSDKKGTRVTFLPSPETFKITDFDFDRLEHRFRELAFLNSGVRLLLTDARH
                     EEEKQVELFYEGGIAAFVRYLDRTKAALFPDPIAIHGQRDDIGIDIALEWNDSYYEQV
                     LAFTNNIPQRDGGTHMAAFRAALTRTLNNYAEKSGMLKKEKVSLTGDDMREGLTAIVS
                     VKLPDPKFGSQTKDKLVSSEVRQPMESLMADKLAEWLEENPGHARSIVGKIIDAAAAR
                     EAAKRARELTRRKGVMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRNREV
                     QAILPLKGKILNVERARFDRMLSSKEVGTLIQAMGTGIGRDDFNIEKLRYHKVVIMTD
                     ADVDGAHIRTLLLTFFYRQMPEIIERGHLYIAQPPLYKVAKGRSEVYLKDDTKLDEYL
                     VDAGIGLMRLETGEGTRFGDDLRGLADHARRMRSLMRYVPRRYDPAIIEALALTAALD
                     PELKASERAAAAARAAAWLQAGDADATWSGEASDEGNYVLKRLWRGVTDYHVVDHKFL
                     VSAEARKLHGLALEQADTYAAQSRLVSLKSAAPAAEGDGETEDEDEPHTRVEGVPRSD
                     RAPPPDAKGVLVRRPTELLEGILAAGRKGLSIQRYKGLGEMNADQLWETTLDPDNRSL
                     LQVEVAQADVADEIFTRLMGDVVEPRRDFIQENALNVANLDV"
     gene            complement(6934..8019)
                     /gene="recF"
                     /locus_tag="VF589_00030"
     CDS             complement(6934..8019)
                     /gene="recF"
                     /locus_tag="VF589_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX8255989.1"
                     /translation="MHIARLSLADFRSYADAVLAPGPGLVILTGENGAGKTNILEAVS
                     LLSPGRGLRGATLSEIARTGGQSGFAVAARLGASAGGKDGVDIGTGTSAAAPERRQVR
                     INGAPASATSLAEWLSVLWLTPAMDRLFIEGAGGRRRFLDRLVLALAPGHAVHAARYE
                     AAMRARNKLLTEAMPDPAWLGALEARMAEHGAALAEARTATVAALSERLAAAPEGPFA
                     RAEIALDGWRDHGGLVGELARGRSRDAAAGRTLSGPHRTDLAVAHAGKRQPAASCSTG
                     EQKALLLGLVLAHADLVAERAGRRPILLLDEVAAHLDPSRRAALFERLAAGGGQVWMT
                     GTEPSLFAGTGGGATWLNVAGGAVRLA"
     gene            8122..8889
                     /locus_tag="VF589_00035"
     CDS             8122..8889
                     /locus_tag="VF589_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8255990.1"
                     /translation="MRFLLALALSLAPHAAHAGTRATYVDGERKKIVIEVADNGDARI
                     TSEGSEPYGVLRGGEFYTVGGPKGAPEVARVADVAAALGQVMPPIFKEMLAAAEKGAP
                     ASKLRIEPRGSRKSGGRDGLVFAVYGMDDSKPDEASEIVVSRDPALKPVGRAVEGLMV
                     GSTLMLAGLIGPAAAEMAGDMRQVFALGTPLDMAGKLKLVALEEAEVPAAATQLPARP
                     RTVAEIVATMKAEMASQAAAAEPAAEEAAPTERGPER"
     gene            complement(8957..9280)
                     /locus_tag="VF589_00040"
     CDS             complement(8957..9280)
                     /locus_tag="VF589_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8255991.1"
                     /translation="MICRLWRGWTTLGNADAYEKLVRGEVIPAIEARRIPGFRHNDLM
                     RRELEAEIEFATLMWFDSIEAIKAFVGEDHEASHVPAAARALLSRFDERAAHFELLDR
                     RPQHG"
     gene            complement(9351..10109)
                     /locus_tag="VF589_00045"
     CDS             complement(9351..10109)
                     /locus_tag="VF589_00045"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEX8255992.1"
                     /translation="MRMFAAALLIAGLVPSAAAAAAQPVQVAKADPVRLDKARALLAL
                     SHPSELVLDANMRGWEAGIAKATAMAPGVDQLEAKYPGIVKAAIEAARPAAREFCAAF
                     AQRSIALMAGVVADRLTAAELDEALSFFRTPAGGKMVRAMLGNIDTKTMVNDMHARAQ
                     AANGEPIVTVENVNRATQAAIRTTFAQTPAEEQVAIMRFGVSPAGKKMFAAIAEADQQ
                     ILVLVNKPDPAWMAQQNETITRAMVEFVDRKKRI"
     gene            complement(10112..11245)
                     /gene="dnaN"
                     /locus_tag="VF589_00050"
     CDS             complement(10112..11245)
                     /gene="dnaN"
                     /locus_tag="VF589_00050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX8255993.1"
                     /translation="MKATIERATLLRSLGHVQSVVERRNTIPILSNVLIEASEGEGPG
                     GAGAGIRLMATDLDLQVNETVEANVTQPGATTVSAHTLFDIARKLPEGSQVELNASEG
                     KMQVNAGRARFNLATLPRDDFPVIAEGDLPTRFELPAATLRQIIDKTRFAISTEETRY
                     YLNGIFLHVSDEALPVLKAAATDGHRLARVTVPRPDGAQGMPDVIIPRKCVAELRKLL
                     DEVDGTVQVSLSESKVRFGLGNAILTSKLIDGTFPDYSRVIPTANDKLLRIDPRSFEE
                     GVDRVATIASEKTRAVKMALDRDRITLSVTSPENGTAAEEVPGDYGSEAFEIGFNARY
                     LLDILGQIDGDIVEVHLADAAAPTLLRENDKASALYVLMPMRV"
     gene            complement(11393..12043)
                     /locus_tag="VF589_00055"
     CDS             complement(11393..12043)
                     /locus_tag="VF589_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX8255994.1"
                     /translation="MRKYVLAALLAGTLATPAMAQEASPFTGFRVEGIVGYDSLRSGQ
                     QDNGVDTNDNEGDESVDGIAFGVGAGYDFDAGGVVLGVEAEFTESTGDQDTDETIDGI
                     NYRAGVATGRDLYIGGRIGVRAAPSTLVYGKAGYTNTSIEADFEDDTDRIEFDTNVDG
                     WRLGAGVEQLFGPNMYGKLEYRYSNYNNLEVGKDLDIERDIDLDRHQIVAGVGFRF"
     gene            12178..12681
                     /locus_tag="VF589_00060"
     CDS             12178..12681
                     /locus_tag="VF589_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8255995.1"
                     /translation="MRFLLFLLLFLSLTAPVQAQRQPLGIFFQWGAFREQGAQHCFAI
                     AEPERAPKAGAWRPSVAVGYWPRRGVRGQVHFRLSREKRAGSAVLLRIDDRTFQLIGG
                     GIDAWAPDRQADAEIVAAMRSGVDMTIETRSERGALIREHYRLRGAATAIDAAAIACA
                     GAAGKKP"
     gene            12704..13906
                     /gene="rlmN"
                     /locus_tag="VF589_00065"
     CDS             12704..13906
                     /gene="rlmN"
                     /locus_tag="VF589_00065"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX8255996.1"
                     /translation="MTAAMQIPGHFDPVPVPRAPLADGDPRPDLVGLSREAIRLALEE
                     AGLEPRQAKLRAKQIWHQIYNRGATCFEAMSDIAKPQRGWFAERFRIGRPEVVEHLVS
                     TDGTRKWLLRTPDGNDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLTA
                     GEIVGQVMLARDTLGEWPSQPEGRMLSNIVMMGMGEPLYNFDNVRDALKIVMDGDGLG
                     LSRRRITLSTSGVVPMMERAGEEIGVNLAVSLHAVTKEVRDEIVPLNRKYGIEELLQA
                     CADYPGVNNARRITFEYVMLKDKNDSDEDARELVRLIRHYKLPAKVNLIPFNPWPGAA
                     YECSSPERVQRFSDIIFEAGISAPVRRPRGRDIMAACGQLKSAAEKKSRAELDRMAAE
                     KQAALAAA"
     gene            13988..15943
                     /locus_tag="VF589_00070"
     CDS             13988..15943
                     /locus_tag="VF589_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX8255997.1"
                     /translation="MASTPLERSPVEEPKPVSTVVRAHDWGSTPLGPIASWPLSLRFA
                     LGVCEHCSLAAAIYWGPDLRLIYNDAWQALMPDGTSGLGLPAAETANSLWPIVADTVE
                     QVLATGTGAFVREQPLVLGRDGVSQETFWNCSILPLLDEHGRIAGILNQANEVTRAVL
                     AERRLSFQIELADRLRGLRQSSAIKSAAIEMLGQYVRAARVGYAELDADEQQVTVRRD
                     WTRNASTANLAGMSAPIGSFGEGAAEFLRAGEPLAIGDLGEIPEVSAQHAEGWAVIGT
                     RAMIVVPLVREGALKALLYVHDPEPRAWKRSEVAMARDVAERTWDALERAQAEQSLRD
                     SEDHYRHAVELNPQVSWTSLPDGQLNRVSRRWRDWTGGAGTGDSWAEGLHPDDRQRTF
                     DVWRHCVATGEPYDIEHRVQHRDGSYRWARSRAFPRYNDKGEICLWYGTTEDIDERRR
                     AEEHQRLLINELNHRVKNTLATVQAIAFQTLKGDIPLEEARRRFEARLMALSRAHNLL
                     TEQNWGGAPLDRVVRDSTGHLPHDRFEVEGEPFWLGPRAALALALAFHELSTNAVKYG
                     ALSGEHGKVRISWGVDGEILRLAWKECDGPAVAEPAGRGFGTRLIERGLAADLGGSAE
                     MRFEGDGLLCLIEASVGIVQARENELG"
     gene            15936..16292
                     /locus_tag="VF589_00075"
     CDS             15936..16292
                     /locus_tag="VF589_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8255998.1"
                     /translation="MASQRLRILIVEDEMLVAMNIEDMLLDLGHEVAGLASRLEPALS
                     LAREADFDLAMLDVNLAGQASFPVAAILVERNIPFLFATGYGVKGIAEEYRGHPVLQK
                     PFRSADLNAALEAARG"
     gene            16427..17950
                     /locus_tag="VF589_00080"
     CDS             16427..17950
                     /locus_tag="VF589_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8255999.1"
                     /translation="MQRENQASQAVDVAIVGAGPAGLTAAYLLTKKGYSVTVIEKDPT
                     YVGGISRTVEYEGFRFDIGGHRFFSKSREVVDLWNEILPDDFIERPRMSRIYYEGKFY
                     SYPLRAFEALWNLGIFRSAACMASYARWKLFPHKQPKSFEQWVVNQFGHKLFSIFFKT
                     YTEKVWGMPCDEMSADWAAQRIKGLSLGGAVLDGLKRSLGLNKRKNDGMAVKSLLETF
                     RYPRQGPGMMWDAARDFVVKGGNKVLMGHSLQQMSQDRGTGRWRVLAKTQDGGTVAIN
                     AAHVISSAPMRELAGRIHPLPATLPSALDLKYRDFLTVALMIRSEDLFPDNWIYIHDA
                     RVKVGRVQNFRSWSPEMVPDPELACVGLEYFCFEGDGLWSASDEDLVKLATEELATLG
                     LADPKTVVGGRVVRQEKAYPVYDDSYRDNVEEMRAELEAAYPTLHMVGRNGMHRYNNQ
                     DHAMMTAMLTVRNIEAGTRAYDIWAVNEDAEYHEAGDEGQQAALASERLVPSRLKAA"
     gene            17962..18342
                     /locus_tag="VF589_00085"
     CDS             17962..18342
                     /locus_tag="VF589_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX8256000.1"
                     /translation="MSRITYLRYIVASAGALGVDMALFLAAMAMGLTPFIAAGLGYVA
                     GIGAHWLLSSRAVFVGHVAERGRPRLQQQGLFLASAVVGLAITMGIVGLGNALALDPR
                     LAKLAAIGISFQATYLLRRNLVFA"
     gene            18342..20153
                     /locus_tag="VF589_00090"
     CDS             18342..20153
                     /locus_tag="VF589_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256001.1"
                     /translation="MASSAAPAPDGALTSPERADDRGGLWPFVLAWLVACAILVAMTA
                     PRVVAFSFPDPDDTLRLQQVRDWLGGQSWFDLNQYRMNPPEGAPMHWSRLVDVPIAAV
                     ILLLTPLIGQAGAETAALVLVPLLTLGIVMALTAAIVWRLLGRDHALLASLLVPVSET
                     ATHQLRPMRIDHHGWQMALALVALLAALDPRQRRSGLIAGAAAAVWLQISLEGLPFAI
                     ALVAFFAIRWLIDPRQGERLVATTASLAGCSLLLFLLTKDGPAWAVQHCDAVSPVHLT
                     ILGLGAAGCFALVRSGIGSLPAKLACFAALGAAAVATMAAMAPVCAAGPFARLDPLVD
                     SFWYQRVSEGLPIWRQAPAQMMMTIAFPLIGLFGSWKAYAAPGEDRRGDWATILFLLA
                     AALLTAMMVQRAGAVANLFAIPGGVFLFRRALLQAREVKRVPRRVVATFGALLIVAPS
                     HIISIATLAVIDRERIESTRAAMSCYERAEIRALNALPTGYIAAPMDIAPAIITETPH
                     SIVASGHHRNMTAMHDLIRMFIAPAAEAEQIVRRRSIDYVVVCPGLIEPRLYAAHGPD
                     GLWDQLSKGQPPQWLVPVQVEGAKSLRIWRVAGAEPR"
     gene            20216..21805
                     /locus_tag="VF589_00095"
     CDS             20216..21805
                     /locus_tag="VF589_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256002.1"
                     /translation="MTASHSAPPGHADQPEGWWQHPALLTAVILASALPVLIADIAPL
                     TDLPGHMGRYRVQLDLAESEALQRFFRFEWMLIGNLGIDLLVIPLSKLFGLEGAVKLI
                     VTSIPPLTVAGFLLVARQVHGRIPPTAFFALPFAYGYPFIFGFVNFALSMALAFLAFG
                     FWLHLARLERLRLRAALFVPISGIVWIAHAFGWGTLGVMAFSAELVRQMDRGRSFVGA
                     GFRAVLHSLVIAPPILLMLFWRSGQVGGQTGDWFNWKAKWHWVLWVLRDRWEWFDLGA
                     LIIIVALLVRALRSPRIEFSRNLAASALFLTLVFLLLPRIIFGSAYADMRLVPFLFAV
                     AVLAIRLKGGATLRFAHTAAAVAIAFALIRLAGNTASFRLYDRDYDRELAALEKVPYG
                     ARLVSFVGRGCEEPWAMLRLEHLPAIATLRRHAFTNDQWTMAGAQLLSVEYPAGEPFV
                     RDPSQVVTPVKCRYERWLPIAETLRRLPRHAFDYVWLINAPAHGRELTRGLQPVWRSG
                     TSVLYRVVDRTPPGAAALEPR"
     gene            21802..22833
                     /locus_tag="VF589_00100"
     CDS             21802..22833
                     /locus_tag="VF589_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8256003.1"
                     /translation="MTPTALSIVIPCYNEEACLPELHRRVSAAARAAAGASYEIVLVN
                     DGSRDRSWAAMQDLARQDDRVVAINLSRNHGHQLALTAGLDLCSGERILIIDADLQDP
                     PELLPEMMAEMDRQGADVVYAVRRARAGETAFKKATAKLFYRLLSRMTDVDIPVDAGD
                     FRLMSRRALDALLSLSEQARFIRGMVAWVGFRQVPLAYDRAERFSGETKYPLGKMVAF
                     ALDAVTGFSTAPLRMASHVGLWLVAATLLLILYIGFGWLSGSAIPGWTSLMLVVVVLG
                     AVQMFVLGMIGEYLGRLYIEAKRRPLYIVSDIAGHAQGRPALGHVAHEAETMATSDTP
                     GGKATLPIA"
     gene            complement(22767..23495)
                     /locus_tag="VF589_00105"
     CDS             complement(22767..23495)
                     /locus_tag="VF589_00105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8256004.1"
                     /translation="MERVVYDRMAELDTRHWWYRARRDILAELIRRRITLPPQARILE
                     IGCGTGHNFEMLGRFGTVDAIEIDGAARAIASSRLGRRVGDAPLPDLPGIDDGRYDLI
                     AVLDVVEHIADDRAALASMARKLRPGGSILITVPAFQWMWSAHDVVNHHHRRYTKKML
                     RRAVADAGLRLHFLSYFNSLLFPLAAASRLAGRLTGKKDSDDKLPPRPINALFETLFG
                     LERHAIGRVAFPPGVSLVAIVSAS"
     gene            complement(23509..23886)
                     /locus_tag="VF589_00110"
     CDS             complement(23509..23886)
                     /locus_tag="VF589_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX8256005.1"
                     /translation="MFGQVVRFALTGGFLTVLVAGGYWVTATFFGVDPMLALTINYLL
                     ATGLGYLLHSRWSFKGHGSRDRSAARSARFFIVNTSGFLANQFFVWLLVKQMGGPTWW
                     PVIPILFVTPLVTFSLNRRWVFA"
     gene            23973..25763
                     /locus_tag="VF589_00115"
     CDS             23973..25763
                     /locus_tag="VF589_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcrB/AcrD/AcrF family protein"
                     /protein_id="HEX8256006.1"
                     /translation="MNGKGERVAGSGSLERHWRLWLLIFWSVAAVLLAYRQWNAIQWF
                     ALGDTDDNLRMMQVRGLLGGQDWFDLRQYRLDPPHGANVHWSRLADLPIAGVMLLLKP
                     FMTGAAAEKVAVAFAPMIPMGIAMAATAVNVRRLVAPGAFALAVALLLCAHSTRGMWA
                     PLRIDHHGWQLALLSLVLLGLTDTKRARGGLVAGLASAASLAIGLELLVYLAAAGAVV
                     ALMWIRDGGEARRLATYGASLAGGTAFGYLVFASYANRAPVCDALSPVWLSAMVAAGA
                     LGFLLTRVPAASWQARLALAAAAGGVLAAFFALAWPHCLGRLEGVSPELYGMWLGNVR
                     EARPVYTHGWQVIANVVALPAAGLVGYALMLWRVRREPPALIPWAATAFLAFIPCALL
                     LWQSRAGPAAQLLAVPGATALAWLLIQRALAASNLLVAAGFSVVTFWVVSGLAVQQLP
                     PLLEKPATGHVQAVNRANNLCPTLAALRPVARVPRGYVLTHVDLGPRLVTVTHHDAVA
                     GPYHRNQAAILDVMKSFRGSVDYARSVIERRGVGYVLICPGLSESTVYAKEAPKGFYA
                     QLAGGRVPAWLEPIALPRDSPYRMWRVVRR"
     gene            complement(25753..26382)
                     /locus_tag="VF589_00120"
     CDS             complement(25753..26382)
                     /locus_tag="VF589_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX8256007.1"
                     /translation="MMELFASAFVTFFVIIDPPGCAPIFASLTHEAPPAERRMMAIRS
                     VLIAAGILLFFGLFGEDLLDALGISLAAFRIAGGIMLFLIALEMVFEKRTQRRESRAS
                     EINATPEIEDIAVFPMAIPMIAGPGSIASIMLLMARSDGLDESLIVLGALGAILLLTL
                     LALLAAGPLMRLLGHKVEAMITRILGVILAALAAQFVIDGISVSFQINV"
     gene            complement(26525..27412)
                     /gene="folD"
                     /locus_tag="VF589_00125"
     CDS             complement(26525..27412)
                     /gene="folD"
                     /locus_tag="VF589_00125"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX8256008.1"
                     /translation="MAADLIDGKAYAERLRGRVAAAVPGFVARSGRKPGLAVVLVGED
                     PASQVYVRAKGKATEAAGMVGFEHFLPDHTSQAELIALVERLNRDPAVDGILVQLPLP
                     AAIDDQAVIAAIDPAKDVDGFHVVNAGRLAVGEPGLVPCTPLGCLMLLKDRLGDLSGL
                     EAVVVGRSNIVGKPMAQLLLRENCTVTIAHSRTRGLPEVVRRADILVAAVGRPQMIRG
                     DWLKPGATVVDVGINRIEIGRGKTRLVGDVEFETAVETAGAITPVPGGVGPMTIAVLL
                     RNTLVAAHARAGYLAPEGL"
     gene            complement(27415..27711)
                     /locus_tag="VF589_00130"
     CDS             complement(27415..27711)
                     /locus_tag="VF589_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX8256009.1"
                     /translation="MALALIDVLIILLTVLWWIIIIQAILSLLVAFNVINTYNDFVRS
                     FLGALDRITEPLYRPFRKILPDFGGIDFSPFVVLILIMIADRLLRGLAADVALA"
     gene            27933..28334
                     /locus_tag="VF589_00135"
     CDS             27933..28334
                     /locus_tag="VF589_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8256010.1"
                     /translation="MKGYVDDIERATIENEDFRRVLYTGKNLQLVLMTLPPGEEIGEE
                     VHEDRDQFFRIEQGRGEVRIDGKANPVEDDFAVIVPAGARHNVVNTGEEPLRLYTLYG
                     PPEHKDGVVHKDKAQAERDHDNDEWTGETTE"
     gene            complement(28341..29486)
                     /locus_tag="VF589_00140"
     CDS             complement(28341..29486)
                     /locus_tag="VF589_00140"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8256011.1"
                     /translation="MPSIATLRNCLYSGAAMLAAVAPGPGFAAPQACRATDAELLAAI
                     RSVHDKQKNVGVQAAIHLDGRLAFSQAHGLADRERKIPVTRNTIFPVASITKAFTGAA
                     LLKAHAAGSFRLDDPIQSYVPEFPVKPELVVTPRRLAAHRAGIRHWGAEREALFARRF
                     DRLDQILPLFKDDPLLAKAGAEYQYSSYGYNLLALALERSVGVEFRRYVRRQILRPLR
                     LSKTRFDDARTPPPGRARLYSFYDLKTYAEIPDPIPVPPRDYSHNMGGGNMSSTAEDL
                     VKFGAALLGPGLLPPAQHDLLFSQPTFGGEPSAMSFGFFAPDAGAERRITISGANPGV
                     QAGLMIYPERGLVVAVLANTWGVGSRDGEMTSDLPKRLADLCWPRPK"
     gene            29586..31679
                     /locus_tag="VF589_00145"
     CDS             29586..31679
                     /locus_tag="VF589_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8256012.1"
                     /translation="MDEDHPAGSATLAAMAGPFQVGAWRAEPAWHRISRSGEIRKLEP
                     RVMRLLAVLAAAPGRPLTREQLLDAVWPNLHVNEDALSRAVSQLRRALSDDPRKPAYI
                     ETVHKGGYCLVASVGSVEAAPAGRTERRSATTPKRMAVAAAALLALLAGGFLLTSLDP
                     AGTSAVKAALVPVPLTSESGREIDPAISRDGRRLAFSASTEAGYDLFWRAIGNDRSVR
                     LTHDGRFAEYPAWSPGGDRIAFLRREADGAAIYIATLATGRLEKLVGLRAWSFGLDWS
                     PDGRTIAYSDSSDGEARSIVLIDLASRVRRTITRAASWRGDGRPVFAPDGRRLAFLRD
                     AGLGLSRIFIADLADPDSARALTVRPEEIKGLDWDPAGGGLVYSARSGGRYGLWRMPV
                     EGGRPQPIPTEGGDLFNPSVSEQGVLVAEAVERDSDVWQARIDGRGAAPLLRSTQDDY
                     DPAHSPYRGRIAFVSSRSGSPELWLARSDGAHARQLTRLGGAHVGGPAWSPDGRQIAF
                     QSQVDGFAAAYILSPGDRPPVLVAGGGKQHHVPLGWGRTGGELFTASGSGSSWTVRRH
                     DIAARRSKALNAVPARLAGVAPDRQSVYLITHDGATLTRVSASSGRAEEFPLPGALRA
                     ADAMRAAGANFYIFVPSPGGTAIHRLRRVGAGLEFDRVASVPGYGQVSVSPDETQLLF
                     TSDRETANDIVLLRL"
     gene            31723..32661
                     /locus_tag="VF589_00150"
     CDS             31723..32661
                     /locus_tag="VF589_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX8256013.1"
                     /translation="MAKGTHDSAPDPRNADILINVNGELKPRDQAMVSVFDSGFVLGD
                     GVWEGLRVHKGRIAFLGRHLDRLWEGAKAIAMDIGLSREALERRLYETLDANGMDESP
                     AQEGVHIRLMVTRGIRSTPYQDPRVVISAPTIVIIPEYKAPLPVTIERGIRLFTVHVR
                     RGDPAVQDPKLNSHSKLNDITACIQAATAGYDEALMLDPHGFVATCNSTHFFIVRKGE
                     VWTSTGDYTLGGITRRNVIELCREDGIPVFEKNFSLTDVYGAEEAFVTGTFAGVVPVR
                     EIDGRHISEARGPMVERLQRLYKARIKADVAGRSRP"
     gene            32658..33404
                     /locus_tag="VF589_00155"
     CDS             32658..33404
                     /locus_tag="VF589_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8256014.1"
                     /translation="MTVRIAMWSGPRNISTAMMRSFGSRSDTFVSDEPFYGAFLKDSG
                     ADHPMREQVIAAMDTDWRSVLRTLSGPVPGGTAVWYQKHMWHHMVGPVGYDDFRGFTH
                     AFLIREPARMIASYIEKREAASFDDFGLDRQVEFFDREADRLGQAPPVVDAADVLRDP
                     ERTLGALCTALGIPFQPAMLRWKPGRRDTDGIWASHWYNAVESSTGFVAPEERAAAMP
                     PKARRLAERCRPYYERLAGHRLAAPPGSES"
     gene            33653..34024
                     /gene="rpsL"
                     /locus_tag="VF589_00160"
     CDS             33653..34024
                     /gene="rpsL"
                     /locus_tag="VF589_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX8256015.1"
                     /translation="MPTINQLIRKGREPQKAKSKVPAMEQNPQKRGVCTRVYTTTPKK
                     PNSALRKVAKIRLTNQREVISYIPGEGHNLQEHSVVLIRGGRVRDLPGVRYHVLRGVL
                     DTQGVKDRRQSRSKYGAKRPK"
BASE COUNT         5225 a        11289 c        12070 g         5555 t
ORIGIN      
        1 tccctgcgtg cagggagggg ggcgaggcaa cagaaagggc ggatttgccg ccgcccctcc
       61 accggctctt gcgggcgcgg ctcggttgtc gcagcctgtg gccatgaccc gcgttcgacc
      121 ccgtctggca aggttgcccg cgctcgctat gctggcgctg atgaccgcct cccccagcct
      181 atccgccgaa cgcccgcgcg cccgtcaagc cgggatcgcc gtcggcatcc tcgcgccggg
      241 accgctcaac gcgatcaccg acgtggccgg cgtcgtcgtt ggccatgtca cgctcaaaga
      301 gggcgcggcg atccggaccg gggtgacggc ggtgctgccg caccgcggca acctattcca
      361 ggacaaggtg ccggccggct tcgccgtcgc caacggcttc ggcaagttca tggggtcgac
      421 ccagatcgag gagctgggcg agatcgagac gccgatcctg ttgaccaaca ccttgtcggt
      481 tcccgaagcg gcggcggcgg tggtggagtg gaccttggcg cagccgggca acgagcaggt
      541 gcggtcggtg aacgcagtcg tcggcgagac caatgacggc cagttgaacg acatacgcgc
      601 gcggcgggtg accaaggccg acgggctcgc ggcgatcgcg gcggcgcgcg aggggcgtgt
      661 cgcggaaggc gcggtcgggg cgggcaccgg caccgtggcg ttcggctgga agggcggcat
      721 cggcaccagc tcgcggcggc tgccggccag gctcggcggc tggacggtgg gcgtgctcgt
      781 ccagagcaat tatggcggtg tgctgaccgt cgacggggta cgggtcggcg aggcgctcgg
      841 gcgatatatg ctgaaggacg agctcgaggg cgcggccggg gacggatcgg tgatcgtcgt
      901 cgtcgccacc gacgcgccgc tctccgaccg caatctgaag cggctcgccc gccgcgcctt
      961 tctcggcatc gccaggaccg gctccccgat caccaacggc tcgggcgact atgccgtcgc
     1021 cttttcgacc gcggccgccg tccgccgcac gcccgcccgg cggagcgccg tcgcggggat
     1081 ggaggagctt cccaacgatc gggtgacgcc gctcttccag gccgcggtcg aagcgaccga
     1141 ggaggcggtg ctgaactcga tgttcaaagc ggagaccatg gaagcgaatg gcgcgcgggt
     1201 ggaggcgctg ccggtggccg aggtgctgca gcttcatcgg cgcggcagcg gccgggcgag
     1261 aggggagtga aggtgcgtgc gcttctcgtg ctcgccgcct tctcggcgat gggatctcag
     1321 gcgagtgcga tcgcgacagc cggtcctctg accggcgcct ggggcgggga gcatgttggg
     1381 ctggagctcg atgcggaggg gggaagggtg cgctatgatt gcgccgcagg gaccatcgat
     1441 ggcccagtgc ttcccggtgc ctccggccgc tttcaggcga acggcgccca tcgccccaat
     1501 ctcggaggcc cggagcggca gggccgcgag ccgccgcttt tgcccgcaat ctacacgggg
     1561 accgtccgcg gcgacatcat gaccctcagc gtgcgggtgc cgagcagggc gatggagatc
     1621 ggtcccttta cgctcaggcg gggtgccgag ccgatccttc ttcgctgcct gtgacgggag
     1681 ggagcagcgg ccgccggagg gtcaacagag gccgcttgcc acggaggttc tgtgtgataa
     1741 catcacatga acctttggag ggatggcccg atgcgtttcc tgaccctgtt gccgctcgcc
     1801 gccctggtcc tcaccggaat cgctccggcg ccgatggccg cgcagccggg cgcgcgccgt
     1861 cctgctgcgc tcgacgccga ggccatttgc cggccgttcc tggacgcgtc cttcggcgat
     1921 agcgactcgc gaggccgcta tggcccggca cgagccttgg cgccgccccc gcctccacct
     1981 ccgccgtcgc cccagcagcc gggtgcgccg gccgaggccg ccacggattc ggaggtggtc
     2041 gtcacgggaa ggcgtatcgc tgagccgaac ctgaccgcgg tttcgccggt gacggtggtc
     2101 aattcgcagg aggtccagtt gagggggacc gtccggaccc agaatctgat gaattccggg
     2161 ccgcaggcct ctgcacaggc gccgggctca tatgttcggg atcccgccaa ccgcgagcgc
     2221 tatgccggga aagaagtcgc ggcggtgcag gcggtggccg aggcgccggt gtccaccttc
     2281 gccgtggacg tcgacaccgg cagctatgcc aatgtccggc ggatgctgaa tgcgggcgaa
     2341 atgccgcccc aggcggcgat ccgcaccgaa gagatgctga actatttccg ctacgattac
     2401 agcgcgccgc gcgaccgcag ccgcccgttc acgatcacga ccgacatgac cacgacgccg
     2461 tggcacccga cgacgcgtct gctccgggtc ggcctcaggg gctatgacgt cgctcgctcg
     2521 gagcgaccgg ccgcgaacct cgtcttcctg gtcgatgtgt ccggatcgat ggaggagccc
     2581 aacaagctgc cgctggtcaa atgctcgctg gccttgctgg ccgagcggct gtcgccgcgc
     2641 gaccgcgtct cgatcgtcgt ctatgccggc gccgccggcc gcgtgctcga gccgacttcc
     2701 gacaaaagac gggtgctgga cgtgctggcg cggctccggg caggcggctc caccgccggc
     2761 gcccaaggca tccagctggc ctatgagacc gcacgggccg gcatgatcga gggcggcatc
     2821 aaccgggtcg tgctcgccac cgacggcgac ttcaacgtcg gcgtgaccaa cgaccaggcg
     2881 ctgatcgaca tggtcgagcg cgaacgcgcg gcggggacca cgctgaccac gctcggctac
     2941 ggcaccggca cttacaacga agcgatgatg gagcagatcg ccgaccacgg gaacggcaat
     3001 tacgcctata tagacgggcc gcgcgaggcg cagaaagtgc tcgaccagga gctcgcctcg
     3061 accttgttca cgatcgccgg cgacgtgaag atccaggtcg agttcaaccc ggcctatgtc
     3121 agcgaatatc ggctgatcgg ctacgagaat cgggcgctgg ccgagcagga tttcgacaat
     3181 gacgcggtcg acgcgggcga tatcggggcg gggcaccagg tgaccgcgct ctacgagatc
     3241 gtgccggccg ggagccgggg ctggctgccc ggccgccgct acccggctaa ccgccgcgag
     3301 gcctccggca tgggtaatga cgagctcgcc tggctgcgcc tgcgctacaa gcttccgggc
     3361 gagaaccagt cgcggctgat cgagcggccg gtgccggccg ggctggtgcg tagcgcgcgg
     3421 gcgccggcgg gggatacggc cttcgccgcg gcggtggcgg cctattcgca gcggctgcgc
     3481 ggcgacccgc tgctggccaa tttcagcttc gccgatgccc ggcggctggc cgcccgcgcc
     3541 gacacgcgcg acccgtggcg gcgggagttc gtcggcctga ccgaggcggc cgagcggctg
     3601 acgtcggagc gggcgggcgg cgcggggcgc taagcgctag cccctccggg cgagccagat
     3661 gacgtgccgg gcgccgcggc ccccggcggc gcgcaccggc acctcctcaa ccgcgaagcc
     3721 ggcggcgcgg agccgctggg cgaagttgcg atggggtgcg gccgaccaaa tcgccagtat
     3781 gccgccgggg cgtagcgccg ctttggcgcg ggccagcccg gccggctggt agagccgctc
     3841 attgtcgtca cggacgatgc cgtccgggcc gttgtcgaca tcgagcagga tcgcgtcata
     3901 gccgcggccg gcgtcgatca ggccggcgac atcggcttcg atcagctcga cgcgcggatc
     3961 ggaaaggctt gtgccgaaca gcggcgcgag cggccctcgc gcccaggcga tgacggcggg
     4021 gaccagctcg gcgatctcga tccgggcgtc gggcccgagc gcggccagcg ccgcccgcaa
     4081 ggtgaagccc ataccgagcc cgccgatcag cagcttggga tcccggcgtc cgccgatccg
     4141 ctcgcagacg agggtggcga gcgcttcctc ggagccgctg aggcggctgt tcatcagctc
     4201 gttgccgccg agcatgatcg aaaattcctc agcccgccgg tacagccgaa gctcgccgcc
     4261 gccggggatt tgcgcggtgt cgatcagcaa ccgggggatc atgagcgcag cgcggcggcc
     4321 ggccgctaca cgtccagatt ggcgacgttc agcgcatttt cctggatgaa gtcgcgcctt
     4381 ggctcgacca cgtcgcccat caggcgggtg aagatctcgt cggcgacgtc ggcctgcgcg
     4441 acctcgacct ggagcagact gcgattgtcc ggatccagcg tcgtttccca aagctgatcg
     4501 gcgttcattt cgcccaggcc cttgtagcgc tggatcgaca ggcccttgcg cccggcggcg
     4561 aggatcccct ccaggagctc ggtcggccgg cggacgagca cgcctttggc gtcgggcggg
     4621 ggagcgcggt cggagcgggg gacgccctcg acgcgggtgt ggggctcatc ctcgtcctcc
     4681 gtctcgccgt cgccttcggc ggccggggcg gcggacttca aggacacgag gcgcgactgg
     4741 gcggcatagg tgtcggcctg ttccagcgcg agtccgtgga gcttgcgcgc ctccgccgag
     4801 accaggaact tgtggtcgac gacatgatag tcggtgacgc cccgccacag ccgcttcagc
     4861 acgtaattgc cctcgtcgct cgcctcgccc gaccaggtcg cgtcggcatc gccggcctgg
     4921 agccaggcgg cggcgcgggc ggcggcggcg gcgcgctcgc ttgccttcag ttcgggatcg
     4981 agtgcggcgg taagcgccag cgcttcgatg atcgcggggt cgtagcggcg cgggacatag
     5041 cgcatcaagc tgcgcatccg ccgggcgtgg tcggcgaggc cgcgcaggtc gtcgccgaag
     5101 cgcgtgcctt cgccggtctc gagccgcatc aggccgatgc cggcatcgac cagatattcg
     5161 tcgagcttgg tgtcgtcctt caaatagact tcggagcggc ccttggcgac tttgtaaagc
     5221 ggcggctggg cgatgtagag gtggccgcgc tcgatgatct ccggcatctg ccggtagaag
     5281 aaggtcagca gcaaggtgcg gatatgggcg ccgtcgacgt cggcgtcggt catgatcacg
     5341 accttgtggt agcgcaattt ctcgatgttg aaatcgtcgc ggccgatgcc cgtccccatc
     5401 gcctggatga gggtgccgac ctccttggag gagagcatcc ggtcgaagcg ggcgcgctcg
     5461 acgttcagga tcttgccttt caagggcagg atcgcctgga cctcgcggtt gcggccctgc
     5521 ttggcggagc cgccggccga atcgccctcg accaggaaca attcgcactt ggccgggtcg
     5581 cgctcctggc agtcggcgag cttgccgggg agggaggcga tgtccatcac gcccttgcgg
     5641 cgggtgagct cgcgcgcgcg cttggcggcc tcgcgcgccg cggccgcgtc gatgatcttg
     5701 ccgacgatcg agcgcgcgtg gcccggattc tcctccagcc actcggccag cttgtcggcc
     5761 atcaggcttt ccatcggctg gcggacttcg gacgagacca gcttgtcctt ggtttgcgag
     5821 ccgaacttcg ggtcgggcag cttcaccgaa acgatcgcgg tcaggccttc gcgcatgtcg
     5881 tcgccggtga ggctcacctt ctccttcttc agcatgccgg acttttcggc ataattgttg
     5941 agcgtccggg tcaaggcggc gcggaaggcg gccatgtggg tgccgccgtc gcgctgcggg
     6001 atgttgttgg tgaaggcgag cacctgctcg taataggaat cgttccattc gagcgcgata
     6061 tcgatgccga tatcgtcgcg ctggccgtgg atcgcgatcg ggtccgggaa caaggccgcc
     6121 ttggtgcggt cgagatagcg cacgaaggcg gcgatgccgc cctcgtagaa gagctcgacc
     6181 tgcttttcct cctcgtgccg ggcgtcggtc agcagcaggc ggacgccgga attgaggaag
     6241 gccagctcgc ggaagcgatg ctcgagccgg tcgaagtcga agtcggtgat cttgaacgtc
     6301 tcgggcgagg gcaggaaggt gacgcgggtg cccttcttgt cggacggccc caccaccttc
     6361 agcggcgctt cggcatcgcc gtggcggaag cgcatgtaat gctcctcgcc gtcccgccag
     6421 atggtgaggt cgagccattc gctgagtgcg ttgacgaccg agacgccgac gccgtgcagg
     6481 ccgccggaga ccttgtacgc attgtcgtcc gaggtgttct cgaacttacc gccggcgtgg
     6541 agctgggtca tgatgacctc ggccgccgaa acgccctcgc cggaatggat gccggtgggg
     6601 atgccgcggc cattatcctc gaccgaaacc gagccgtccg ggttcagggt gatctggatg
     6661 aggtcgcaat ggccggccaa cgcctcgtcg atcgcattgt cggaaacctc gaacaccatg
     6721 tggtgcaggc ccgaaccgtc gtcggtgtcg ccgatataca ttccgggccg cttgcggacc
     6781 gcgtcgaggc ctttcagcac cttgatggat tcggcgccgt aggcgttttc ggtgggggat
     6841 gatgccatgc cgattatata gcgacggggg gggcgaaacc caagggaaaa ggccgagtcg
     6901 cgccggctgg ttcgcgggtg cagcatggcc gcttcaggcg agccggacgg cgccccccgc
     6961 gacgttgagc caggtcgctc cgccgccggt gccggcgaac aaagagggct cggtgccggt
     7021 catccagacc tggccgccgc cggccgccaa tcgctcgaac aaagccgcgc gccggctcgg
     7081 gtcgagatgg gccgcgacct cgtcgaggag caggatcggg cgtctcccgg cccgctcggc
     7141 gacgagatcg gcatgggcca ggaccagccc gagcagcaac gccttctgct ccccggtcga
     7201 gcagcttgcc gccggctggc gcttgccggc atgggcgacg gcgaggtcgg tgcggtgggg
     7261 gccgctcagg gtacggccgg ccgccgcatc gcgggagcgg ccgcgggcaa gctcgccgac
     7321 caggccgcca tggtcccgcc acccatcgag tgcgatctcc gcccgcgcga acggcccttc
     7381 cggggcggcg gccagccgct ccgacaaggc ggcgacggta gcggtccgcg cttccgccag
     7441 ggcagctcca tgctcggcca tgcgcgcttc gagggcgccg agccaggccg ggtccggcat
     7501 ggcttcggtg aggagcttgt tgcgcgcgcg catcgccgct tcgtagcggg cggcgtggac
     7561 ggcatggccg ggggcgagcg ccagcaccag ccgatcgagg aagcggcggc gcccgccggc
     7621 gccctcgatg aacagccggt ccatcgccgg ggtcagccac agcaccgaca gccattcggc
     7681 gagcgaggtc gccgaagccg gcgcgccgtt gatgcggacc tggcggcgct cgggcgcggc
     7741 ggctgaggtg ccggtgccga tgtcgacgcc gtccttgccc cccgcggagg cgcccaggcg
     7801 cgccgccacg gcgaacccgc tttgcccccc ggtgcgggcg atttcggaga gggtggcgcc
     7861 gcgcaggccg cggccggggg agaggagcga gacggcttcg aggatgttgg tcttgccggc
     7921 gccattctcg ccggtgagga tgacgagacc gggccccggc gccagcacgg catcggcata
     7981 ggagcggaaa tcggcaaggg acaggcgggc gatgtgcacc ggccggcttg tagccgcctc
     8041 gccgccgccg cgccaagcgc ggtggacccg gggcggccgc aggcgtaggc tgcggccgac
     8101 cagacccatg gaggcatcgc catgcgcttc ctgctcgcac tcgctttgtc gctcgcgccg
     8161 cacgccgctc atgccggcac gcgcgccacc tatgtcgacg gcgaacgcaa gaagatcgtc
     8221 atcgaggtgg cggacaatgg cgatgcccgc atcaccagcg agggttcgga gccttacggc
     8281 gtgcttcgcg gcggcgaatt ctacacggtc ggcgggccga agggtgcgcc cgaggtcgcg
     8341 cgggtcgccg acgtcgcagc cgcgctcggc caggtgatgc cgccgatctt caaggagatg
     8401 ctggcggcgg cggagaaggg cgcgccggca tcgaagctgc ggatcgagcc gaggggcagc
     8461 cgcaagtcgg gcgggcggga cggactggtc ttcgccgtct acgggatgga cgatagcaag
     8521 cccgatgagg cttcggagat cgtcgtcagc cgcgatcccg cgctgaagcc ggtcggccgg
     8581 gcggtggagg ggctgatggt cggctcgact ttgatgctgg ccggcctgat cggcccggcg
     8641 gcggcggaga tggcgggcga catgcgccaa gtgttcgcgc tcggcacgcc gctcgacatg
     8701 gcgggcaagc tcaagctggt ggcgctggag gaggcggagg tcccggccgc ggcgacgcag
     8761 ctcccggcgc ggccgcggac cgtggcggag atcgtcgcga cgatgaaggc ggagatggcg
     8821 tcgcaggcgg cggcggcgga gcctgcggcc gaagaagcag ccccgaccga gcgcggaccc
     8881 gaacgataac gggctactcg atttcagcgg accgccgcgg gcatgacagg aattgagctt
     8941 ggcgcagagc cttgcgtcat ccgtgctgtg ggcggcggtc gagcagctcg aaatgggcgg
     9001 cgcgttcgtc gaagcgggac agcaatgcgc gggcggccgc cggaacgtga ctggcttcat
     9061 gatcttcgcc gacgaacgcc ttgatggctt cgatgctgtc gaaccacatc aaagttgcga
     9121 actcgatctc ggcttcaagc tcgcgccgca tcaggtcgtt gtggcgaaag ccggggatac
     9181 gtcgcgcctc gatggcgggg atcacctctc cccgcaccag cttttcatag gcatccgcgt
     9241 tgccgagggt cgtccagccg cgccacaggc ggcagatcat ggggcggccc tcccggcggc
     9301 cgccggcgca ggtgcggcgg ggactgtcat acccaccatc tgccgctgtc ctagatgcgc
     9361 ttcttgcgat cgacgaactc gaccatggca cgcgtaatgg tctcgttctg ctgcgccatc
     9421 caggcgggat ccggcttgtt gaccagaacc agtatctgct ggtccgcctc ggcgattgcg
     9481 gcaaacatct ttttgcccgc cggactgacg ccgaagcgca tgatcgcgac ctgctcttcg
     9541 gcaggggttt gcgcgaaggt ggtgcgtatc gccgcttgcg tcgccctgtt cacgttttcc
     9601 acggtgacga tcggttcgcc attcgccgcc tgggcccgcg catgcatatc gttgaccatc
     9661 gtcttcgtat cgatgttgcc caacatggcc cggaccatct ttccgcctgc cggggtgcga
     9721 aagaaactca gcgcctcgtc cagttcggcg gcggtgagcc ggtcggccac cacgcccgcc
     9781 atcagagcta tgctgcgctg ggcgaaggcg gcgcagaatt cacgggccgc aggccgcgcg
     9841 gcctcgatcg ccgctttgac gatgccggga tatttcgcct cgagctggtc gaccccggga
     9901 gccatcgcgg tcgccttggc gatacccgcc tcccagcctc tcatattggc atcaagcaca
     9961 agctcggagg ggtggctgag cgcgagcagc gcgcgggcct tgtcgagccg gacgggatcg
    10021 gccttcgcga cctggaccgg ctgcgcggcc gcggccgcgg cgctcggcac gagcccggcg
    10081 atcagcaggg ccgcggcaaa catgcgcatg ctcagacccg catcggcatc agcacgtaga
    10141 gcgcggatgc cttgtcgttc tcgcgcagca gagtcggggc ggctgcgtcg gcgaggtgga
    10201 cctcgacgat gtcgccgtcg atctggccca atatgtcgag caggtagcgc gcgttgaacc
    10261 cgatctcgaa agcctcggag ccgtaatcgc ccggtacttc ctcggccgcg gtgccgtttt
    10321 ccgggctggt gacggagagg gtgatgcggt cgcggtccag cgccatcttg accgcccggg
    10381 tcttctcgga ggcgatggtg gcgacgcggt cgaccccttc ctcgaagctg cgcggatcga
    10441 tgcggagcag cttgtcgttc gcggtcggga tgacgcggct gtaatcgggg aaggtgccgt
    10501 cgatcagctt tgaggtcagg atcgcattgc cgaggccgaa gcggaccttg gattcggaca
    10561 gcgagacctg gaccgtgccg tcgacctcgt cgagcagctt gcgcagctcg gccacgcatt
    10621 tgcgcgggat gatcacgtcc ggcatgccct gggccccgtc ggggcggggc accgtcaccc
    10681 gcgcgaggcg gtggccgtcg gtcgccgccg ccttcagcac cggcagcgcc tcgtccgata
    10741 cgtgcaggaa gatgccgttc agatagtaac gcgtctcttc ggtggagatg gcgaagcggg
    10801 tcttgtcgat gatctgccgg agcgtcgccg ccggaagctc gaaccgggtc ggcaggtcac
    10861 cctcggcgat cacggggaag tcgtcccggg gcaaggtggc gaggttgaag cgggcgcggc
    10921 ccgcattgac ctgcatcttg ccctcggacg cgttcaattc gacctggctg ccctcgggga
    10981 gcttgcgggc gatgtcgaac aaagtgtggg cggagacggt ggtggcgccg ggctgggtga
    11041 cgttggcctc gacggtctcg ttgacctgaa ggtcgagatc ggtggccatc aggcggatgc
    11101 cggctccggc ccccccgggg ccctcgccct cgctcgcctc gatgaggacg ttggagagga
    11161 tcgggatcgt gttcctgcgc tccaccacgg actggacgtg cccgaggctc ctgaggagcg
    11221 tagcccgttc gatcgtcgcc ttcatcaggt cccccaaaag tggtactttg aaggagtatt
    11281 agagccgctt cccccggacg gctagggggt tgttcggcgg cacgaaaaaa gggccggagc
    11341 acgaggcccc ggtccttctt gccggctcga agcaccgctt cgagcggaag actcagaagc
    11401 ggaagccgac gccggccacg atctggtgac ggtcgaggtc gatgtcgcgc tcgatgtcga
    11461 ggtctttgcc cacttcgaga ttgttgtagt tcgagtagcg atattcgagc ttgccgtaca
    11521 tgttggggcc gaacagctgc tcgacgccgg cgcccagtcg ccagccgtca acattggtgt
    11581 cgaactcgat gcggtcggtg tcgtcctcga aatcggcctc gatgctggtg ttggtataac
    11641 cggccttgcc gtagacgagg gtgctgggcg cggcgcggac gccgatgcgg ccgccgatat
    11701 agaggtcgcg gccggtggca acgccggcgc ggtaattgat gccgtcgatc gtctcgtcgg
    11761 tatcctgatc gccggtcgat tcggtgaact cagcctcgac gccgaggacg acgccaccag
    11821 cgtcgaaatc gtagccggcg ccgacgccga acgcgatgcc gtctaccgac tcgtcgcctt
    11881 cattgtcgtt ggtgtcgacg ccgttatcct gctgaccgga ccgaagcgaa tcgtaaccga
    11941 cgatgccttc gacgcggaag ccggtgaagg gcgaggcctc ctgcgccatg gcaggggtgg
    12001 cgagggtgcc ggcaagaagg gcggccaaga catatttgcg cattgtagaa ctccggttgt
    12061 tagtggaccc taaggttcgg cgggcccagc gagtcgccta gcattaagtt tcatgaacct
    12121 cagataaacg gaaaatcctg ttgcactcct gcaacataac gtggatcagt tgctggtatg
    12181 cgctttctgt tattcctgct tctattcctg agtcttaccg cgccggtgca ggctcagcgc
    12241 cagccactcg gaatattctt ccagtggggc gcctttcgcg aacaaggcgc gcagcattgc
    12301 ttcgccatcg ccgagccgga gcgggcgccg aaggccggcg cctggcggcc gtcggtcgcg
    12361 gtcggctatt ggccgcgccg cggcgtcagg ggccaggtgc atttccgcct gagccgggag
    12421 aagcgcgcgg gatcggcggt gctgctgcgg atcgacgacc gcaccttcca gctgatcggc
    12481 ggcggcatcg atgcctgggc gccggatcgg caagccgatg cggagatcgt ggcggcgatg
    12541 cgcagcgggg tcgacatgac gatcgagacg cgatcggagc ggggggcgct gatccgcgag
    12601 cattaccgcc ttcgcggcgc cgctaccgcg atcgacgccg ccgcgatcgc ctgcgccggc
    12661 gcggctggaa aaaagccgtg atcttcccta catggcgcgg atcatgacag ccgccatgca
    12721 gatacccggg catttcgatc ccgtgcctgt cccgcgcgca ccgctcgcgg acggcgaccc
    12781 gcgccccgat ctggtcgggc tttcgcgcga ggcgatccgg ctcgcgctcg aggaggccgg
    12841 gctggagccc cgccaggcga agcttcgcgc caagcagata tggcaccaga tctacaatcg
    12901 cggcgcgaca tgtttcgagg cgatgagcga catcgccaag ccgcagcgcg gctggttcgc
    12961 cgaacgcttc cggatcgggc ggcccgaagt ggtcgagcat ctcgtctcca ccgacggcac
    13021 ccgcaaatgg ctgctgcgca cgcccgacgg caacgatttc gagatggtgt tcatccccga
    13081 tgccgaccgt ggcacgctgt gcgtgtcgag ccaggtcggc tgtacgctca attgccgctt
    13141 ctgccatacc ggcaccatgc ggctggtcag gaacctcacc gcgggcgaga tcgtcggcca
    13201 ggtgatgctg gcgcgtgaca cgctcggcga atggccctcc cagccggaag gacgcatgct
    13261 ttcgaacatc gtgatgatgg ggatgggcga gccgctctac aatttcgaca acgtccggga
    13321 tgcgctgaag atcgtgatgg acggcgacgg ccttggcctt tcgcgccgcc gcatcaccct
    13381 ctccacctcc ggcgtggtgc cgatgatgga gcgcgctggc gaggaaatcg gcgtcaacct
    13441 cgccgtctcg ctgcacgcgg tgaccaagga ggtacgcgac gagatcgtgc cgctgaaccg
    13501 caaatacggc atcgaggagc tgctgcaggc ctgcgcggat tatcccgggg tcaacaatgc
    13561 ccgccgcatc acgttcgaat atgtgatgct caaggacaag aatgacagcg acgaggatgc
    13621 ccgcgagctg gtgcggctga tccgccacta caagctgccc gccaaggtca acctcatccc
    13681 gttcaaccct tggccgggcg cggcttacga atgttcgagc ccggagcgag tgcagcgttt
    13741 ctccgacatc atcttcgagg cggggatcag cgcccccgta cgccggccgc gcggccgcga
    13801 catcatggcg gcctgcggcc agctcaaatc cgccgcggag aagaagagcc gggccgagct
    13861 cgaccggatg gcggccgaga agcaggccgc gctcgccgcc gcttaggcgc gccagcggct
    13921 tttctcccct ttctgaaccc ttcgggcggc gttatggagc cagcttccgt cggagtctgc
    13981 gtcgcttatg gcttctactc ccctcgagcg ttcgcctgtc gaggagccga agccggtcag
    14041 cacggtggtg cgggcgcatg actggggttc cacgccgctc gggccgatcg caagctggcc
    14101 gctgagcctg cgcttcgcgc tcggcgtgtg cgagcattgc agcctcgcgg ccgccatcta
    14161 ctggggaccc gacctccggc tgatctacaa tgacgcctgg caggcgctga tgccggacgg
    14221 cacgtccggt ctcggcctgc ccgccgccga gacggcaaac tcgctgtggc cgatcgtcgc
    14281 ggacacggtc gaacaggtgc tggcgaccgg caccggcgcg ttcgtacgcg agcagccgct
    14341 cgttctcggc cgcgacggcg tcagtcagga gacgttctgg aattgcagca tcctgccgct
    14401 gctcgacgaa cacgggcgga tcgccggcat cctcaaccag gccaacgagg tcacgcgggc
    14461 ggtgcttgcg gagcggcggc tgtcattcca gatcgagctg gccgaccggc tgcgggggct
    14521 gcgccaatcg agcgcgatca agtcggccgc gatcgagatg ctgggccaat atgtgcgcgc
    14581 ggcgcgggtc ggctatgccg agctcgacgc ggacgagcag caggtcaccg tgcgacgcga
    14641 ctggacgcgc aatgcctcga ccgcgaacct ggccggaatg agcgcgccga tcggcagctt
    14701 cggcgaaggc gcggcggaat tcctccgcgc cggggaaccg ctggcgatcg gcgacctcgg
    14761 cgaaatccct gaagtgtcgg cgcagcacgc ggaaggctgg gccgtcatcg gcacgcgggc
    14821 gatgatcgtg gtgccgctgg tgcgggaggg cgcgctcaag gccctgctct acgtccatga
    14881 cccggagccg cgcgcctgga agcggtctga ggtggcgatg gcccgcgacg tcgccgaacg
    14941 gacctgggac gcgctcgagc gggcccaggc cgaacagtcg ctgcgggaca gcgaggacca
    15001 ttaccgtcac gccgtcgagc tcaacccgca agtgtcgtgg acctcgcttc ccgacggaca
    15061 gctcaaccgc gtctcccgcc gctggcgcga ctggaccgga ggggcgggca ccggcgacag
    15121 ctgggccgag ggcctccatc ccgacgaccg gcagcgcacc ttcgacgtgt ggcgccactg
    15181 cgtcgcgacc ggcgagccgt acgacatcga acatcgcgtc cagcatcgcg acggcagcta
    15241 ccgctgggcc cgatcgcgcg cctttccccg ctataacgac aagggcgaga tctgcctctg
    15301 gtatgggacc accgaggaca tagacgagcg ccgccgagcg gaggagcatc agcggctgct
    15361 gatcaacgag ctcaaccacc gggtcaagaa tacgctggcg acggtgcagg cgatcgcctt
    15421 ccagacgctc aagggcgata tcccgctgga ggaggcgcgg cgccgcttcg aggcgcggct
    15481 gatggcgctg tcgcgggcgc acaaccttct caccgagcag aattggggcg gggcgccgct
    15541 cgatcgggtg gtgcgcgatt cgacggggca cctcccgcac gaccgcttcg aggtggaggg
    15601 agagccgttc tggctggggc cgcgcgccgc gctggcgctg gcgctcgcct tccacgagct
    15661 cagcacgaac gccgtcaaat atggcgcgct ttcgggcgag cacggcaagg tccgcatcag
    15721 ctggggcgtg gatggcgaaa tcctgcggct ggcatggaag gagtgcgacg gcccggccgt
    15781 cgccgagccc gccggccgcg gcttcggcac caggcttatc gagcgcggcc tcgccgccga
    15841 tctcggcggc agcgccgaga tgcgcttcga gggcgatggt ctgctttgcc tgatcgaggc
    15901 gtcggtgggc atcgtccagg cgcgggagaa cgagcttggc tagccagcgg ctccgaatcc
    15961 tcatcgtcga ggatgagatg ctcgtggcga tgaacatcga ggatatgctg ctcgacctcg
    16021 gccacgaagt ggctgggctg gcgagccggc tggagccggc gctgtcgctc gcgcgcgaag
    16081 ccgatttcga cctcgccatg ctcgacgtca atttggcggg gcaggcgtcc ttcccggtcg
    16141 cggcgatcct ggtcgagcgc aacatcccgt tcctgttcgc gaccggctac ggcgtcaaag
    16201 gcatcgccga ggaatatcgc ggccatccgg tcctccagaa gccgtttcgc tctgccgacc
    16261 tcaatgccgc gctggaggcc gcgcgggggt aagggcgcgc tccccggctc cgggtgcggg
    16321 gctgagcaaa atcgccggtg acccttcaaa agtgaccgta acgtttacca atctttagaa
    16381 cctctgctcc actcatggcg tcaatcatca ggcgggggat ctagagatgc agcgtgaaaa
    16441 ccaggcaagc caggcagtcg atgtcgctat cgtcggcgcc ggcccagcgg gactcaccgc
    16501 cgcctatctg ctgaccaaga agggctattc ggtcaccgtg atcgagaagg atcccaccta
    16561 tgtcggcggg atcagccgca ccgtggaata tgaaggcttc cgcttcgaca ttggcggcca
    16621 ccgcttcttt tcgaagtcgc gcgaggtggt cgacctctgg aacgagatcc tgcccgacga
    16681 tttcatcgag cggccgcgga tgagccggat ctattacgag ggcaaattct actcctaccc
    16741 gctgcgcgcc ttcgaggcgc tgtggaacct cggcatcttc cgctcggccg cctgcatggc
    16801 gagctatgcg cgctggaagc tgttcccgca caagcagccg aaaagcttcg agcaatgggt
    16861 ggtcaaccag ttcggccaca agctcttctc gatcttcttc aagacttaca cggagaaggt
    16921 gtgggggatg ccgtgcgacg agatgtcggc ggactgggcg gcgcagcgga tcaaggggct
    16981 gagcctcggc ggggcggtgc tcgacgggct gaagcgctcg ctgggcctca acaagcgcaa
    17041 gaatgacggg atggcggtca agagcctgct cgagaccttc cgctatccgc gccaggggcc
    17101 gggcatgatg tgggacgcgg cacgcgactt cgtcgtcaaa ggcggcaaca aggtgctgat
    17161 gggccattcg ctgcagcaga tgtcgcagga ccgcggcacc ggccgctggc gggtgctggc
    17221 caagacgcag gacggcggca ccgtcgcgat caacgcggcg cacgtcatct cgtccgcgcc
    17281 gatgcgggag ctcgccgggc gcatccatcc gctgccggct accttgccgt cggcgctcga
    17341 cctcaaatat cgcgacttcc tgacggtggc gctgatgatc cgctccgagg acctgttccc
    17401 ggacaattgg atctatatcc acgacgcgcg ggtgaaggtc ggccgggtgc agaatttccg
    17461 cagctggtcg cccgagatgg tgcccgatcc agagctcgcc tgcgtcggcc tcgaatattt
    17521 ctgcttcgaa ggggacgggc tgtggtcggc atccgacgag gatctggtca agctcgccac
    17581 cgaagaactg gcgacgctcg gcctggccga cccgaagacc gtggtcggcg ggcgcgtggt
    17641 gcggcaggag aaggcctacc ccgtctatga cgacagctac cgcgacaatg tcgaggagat
    17701 gcgcgccgag ctcgaagccg cctatccgac gctgcacatg gtcggccgca acggcatgca
    17761 ccgctacaac aatcaggacc atgcgatgat gacggcgatg ctgaccgtcc gcaacatcga
    17821 ggcggggacc cgcgcctacg acatctgggc ggtcaacgag gatgccgaat atcatgaagc
    17881 cggtgatgag ggccagcagg cggcgcttgc ttccgagcgg ctcgtcccga gccggctgaa
    17941 ggccgcctga ggcgcatcgc catgagccgg atcacctatc tccgctacat cgtcgccagc
    18001 gccggagcgc tgggggtcga catggccctg ttcctcgccg cgatggcgat ggggctcacg
    18061 cccttcatcg ccgccggcct cggctatgtc gccggcatcg gggcgcactg gctgctctcg
    18121 agccgcgccg tgttcgtcgg ccatgtcgct gagcgcggca ggcctcggct ccagcagcag
    18181 gggctgttcc ttgcctcggc cgtggtcggg ctcgcgatca cgatgggcat tgtcggcctt
    18241 ggtaatgcgc tcgcgctcga tccgaggctg gccaagttgg cggcgatcgg gatcagcttc
    18301 caggccacct atttgctgcg gcgcaacctg gtcttcgcgt gatggcgagc tcggcggcac
    18361 cggcgcccga tggggcgctc acgtcacccg aacgcgcgga tgaccgcggc ggcctgtggc
    18421 cgttcgtgct ggcctggctg gtcgcttgcg cgatcctcgt cgccatgacg gcgccgcggg
    18481 tggtggcgtt cagcttcccc gaccccgacg atacgctgcg cctccagcag gtgcgcgact
    18541 ggctcggcgg ccagagctgg tttgacctca atcaatatcg catgaacccg cccgaagggg
    18601 cgccgatgca ctggtcgcgg ctggtcgacg tgccgatcgc ggcggtgatc ctgctgctga
    18661 cgccgctgat cggccaggcg ggcgccgaga ccgcggcgct ggtgctggtg ccgctgctga
    18721 cgctcgggat cgtgatggcg ctgacggcgg cgatcgtctg gcggctgctg ggccgcgacc
    18781 atgctttgct cgccagcctg ctggtgccgg tgtccgaaac ggccacccac cagctccggc
    18841 cgatgcggat cgaccatcac ggctggcaga tggctctggc gctggtcgcc ctgctcgcgg
    18901 cgctcgaccc gcggcagcgc cggtcgggcc tgatcgccgg cgcggcggcc gcggtctggc
    18961 tgcaaatctc gctcgagggg cttccattcg cgatcgcgct ggtcgccttc ttcgcgatac
    19021 gctggctgat cgacccccgc caaggcgagc ggctggtcgc gacgacggcc tcgctcgccg
    19081 ggtgcagcct gctgctgttc ctcctcacca aggacgggcc cgcctgggcg gtccagcatt
    19141 gcgacgcggt ctcgccggtt cacctgacga tcctcggcct cggcgcggcc ggatgcttcg
    19201 cgctggtgcg gagcgggatc ggttcgctgc cggccaagct cgcctgcttc gccgcgctcg
    19261 gcgcggccgc ggtcgcgacc atggcggcga tggcgcctgt ttgcgcggcc gggccgttcg
    19321 cccggctcga tcctttggtc gacagcttct ggtatcagcg cgtcagcgag gggctgccga
    19381 tctggcggca ggcgccggcg cagatgatga tgacgatcgc cttcccgctg atcggcctgt
    19441 tcggcagctg gaaggcctat gccgcccccg gcgaagaccg ccgcggcgac tgggcgacga
    19501 tcctgttcct gctggccgcg gcgttgctga cggcgatgat ggtgcagcgc gccggcgccg
    19561 tcgccaatct gttcgcgatc cccggcggcg tcttcctgtt ccgccgcgcg ctgctgcagg
    19621 cccgcgaggt caagcgggtg ccacggcggg tggtggcgac gttcggagcg ttgctgatcg
    19681 tcgcgccgag ccatatcatc tccatcgcaa cgctggcggt aatcgaccgc gagcggatcg
    19741 agagcacgcg ggccgcgatg agttgctacg agcgggccga aatcagggca ctgaacgcgc
    19801 tcccgaccgg ctatatcgcc gcgccaatgg acatcgcgcc cgcgatcatc acggagaccc
    19861 cgcacagcat cgtggcgagc ggccaccacc gcaacatgac ggccatgcac gacctcatca
    19921 ggatgttcat cgcgccggcc gcggaggccg agcagatcgt acgccggcgg tcgatcgatt
    19981 atgtggtggt ctgtccgggg ctgatcgagc cgcggctcta tgccgcccac ggacccgacg
    20041 ggctgtggga ccagctcagc aaaggccaac ccccgcaatg gctggtgcca gtccaggtcg
    20101 aaggggcgaa gtcgctgcgc atctggcgcg tcgcgggcgc cgagccgcgc tgatttgagt
    20161 tgaattcgcc cgcatttgcg ggaaggcatc gggcctgaac cgcccgagag ccgccttgac
    20221 cgcatcgcat tccgcacctc cgggccatgc cgaccagccg gaaggctggt ggcagcatcc
    20281 ggcgctgctg accgccgtca tcctggcctc ggcgctgccg gtattgatcg cggacattgc
    20341 gccgctgacc gacctgcccg ggcatatggg gcgctaccgg gtccagctcg acctcgcgga
    20401 gtcggaggcg ctgcagcgct tcttccggtt cgaatggatg ctgatcggca atctcggcat
    20461 cgacctgctc gtcatcccgc tgtcgaagct gttcgggctg gagggggcgg tgaagctgat
    20521 cgtcaccagc atccccccac tcacggtggc gggcttcctg ctggtggcgc gccaggtcca
    20581 cggccggatc ccgccgaccg cctttttcgc tttgcccttc gcctacggct acccgttcat
    20641 cttcggcttc gtgaacttcg ctttgtcgat ggcgctcgcc ttcctcgcct tcggcttctg
    20701 gctgcacctc gcgcggctcg agcggctcag gctgcgggcc gccttgttcg tgccgatatc
    20761 gggaatcgtc tggatcgcgc acgctttcgg gtggggtacg ctcggcgtga tggccttttc
    20821 ggccgagctc gtccggcaga tggaccgcgg ccgaagcttc gtcggggccg gcttccgcgc
    20881 cgtccttcac agcctcgtca tcgcgccgcc gatcctgttg atgctgttct ggcggagcgg
    20941 ccaggtcggc ggccagaccg gcgactggtt caactggaaa gccaagtggc actgggtcct
    21001 gtgggtgctg cgcgaccgct gggaatggtt cgatctcggc gcgctgatca tcatcgtggc
    21061 gctgctggtg cgggcgcttc ggagcccccg catcgaattc tcccgcaacc tcgccgcgtc
    21121 ggcgctgttc ctgacattgg tcttcctgct gcttccccgc atcatcttcg gctcggccta
    21181 tgctgacatg cggctggtgc catttctgtt cgccgtcgcc gtgctcgcca tccgtttgaa
    21241 gggcggggcg acgctccgct tcgcgcacac ggccgcggcc gtcgcgatcg ccttcgcgct
    21301 gatccgcctt gccgggaaca ccgccagctt ccggctctac gaccgcgact atgaccgcga
    21361 gctggcggcg ctcgagaagg tcccctatgg cgcgcggctg gtctccttcg tcggccgcgg
    21421 ctgcgaggag ccgtgggcga tgctgcggct cgaacatctg ccggcgatcg ccacgctgcg
    21481 ccggcatgcc ttcaccaacg accagtggac gatggcgggg gcgcaattgc tctcggtcga
    21541 gtatccggcg ggcgagccgt tcgtccgcga tccttcgcaa gtggtgacgc cggtcaagtg
    21601 ccgctacgag cgctggctgc cgatagccga gacgctgcgc cgcctgccgc gccacgcctt
    21661 cgactatgtc tggctgatca acgcgccggc ccatggccgg gagctcaccc ggggtctaca
    21721 accggtctgg cgcagcggca ccagcgtgct ctatcgggtc gtcgaccgga cgccgcccgg
    21781 cgccgccgcc ctggagccac gatgaccccc accgcattgt cgatcgtcat cccctgctac
    21841 aatgaagagg cctgcctgcc agagcttcac cggcgggtca gcgcggccgc gcgggcggcg
    21901 gcgggcgctt cctacgagat cgtgctggtc aacgacgggt cccgcgaccg gagctgggcg
    21961 gccatgcagg atctggcgcg gcaggacgat agggtggtcg cgatcaacct gtcgcgcaac
    22021 cacggccacc agctcgccct gaccgccggc ctcgacctgt gctcgggcga gcgcatcctg
    22081 atcatcgacg ccgacctcca ggacccgccc gagctgttgc cggagatgat ggccgagatg
    22141 gaccggcagg gcgccgacgt cgtctatgcg gtgcgccgcg cccgggcggg cgagaccgcg
    22201 ttcaagaagg ccaccgccaa gctcttctac cggctgctgt cgcggatgac tgacgtcgac
    22261 ataccggtcg atgccggcga cttccgcctg atgagccggc gcgcgctgga cgccttgctg
    22321 agcctgtccg aacaggcgcg cttcatccgg ggcatggtcg cctgggtcgg ttttcggcag
    22381 gtgccgctcg cctatgaccg ggcggagcgc ttctcgggcg agaccaaata tccgctcggc
    22441 aagatggtcg cgttcgcgct tgacgcggtg accggctttt cgaccgcgcc gctccgcatg
    22501 gccagccatg tcggcctgtg gctggtggcg gcgaccttgc tgctcatcct ctatattggc
    22561 ttcggttggc tgagcggcag cgccattccg ggctggacct cgctgatgct ggtggtggtg
    22621 gtgctcggtg cggtgcagat gttcgtgctc ggcatgatcg gcgagtatct cggacggctt
    22681 tatatcgaag cgaagcgccg gccgctctac atcgtctccg atatagccgg gcacgcgcag
    22741 gggcggccgg cgctaggcca tgtcgctcat gaagccgaga cgatggcgac cagcgacacg
    22801 ccgggcggga aggcgaccct gccgatcgcg tgacgctcca gcccgaacag ggtctcaaac
    22861 aaggcattga tcggccgcgg cggcagcttg tcgtcgctgt cctttttgcc ggtcaggcgg
    22921 ccggcgagcc gcgacgccgc cgccagcgga aagagcaggc tgttgaagta gctgaggaag
    22981 tggagccgaa gcccggcatc cgcgaccgcc cgccgcagca tcttcttggt gtagcggcgg
    23041 tgatgatggt tgaccacgtc gtgggcgctc cacatccatt ggaaggcggg aacggtgatg
    23101 aggatggaac cgcccggcct cagcttgcgc gccatgctgg cgagcgccgc ccggtcgtcg
    23161 gcgatatgtt cgacgacgtc gaggacggcg atgaggtcgt agcggccgtc gtcgatcccc
    23221 ggcaggtcgg gcagaggcgc gtcgccgacg cggcggccga ggcggctgct ggcgatggcg
    23281 cgggcggcgc cgtcgatctc gatcgcatcg accgtgccga accggccgag catctcgaaa
    23341 ttgtggccgg tgccacagcc gatctccagg atgcgcgcct ggggcggcag cgtgatccgg
    23401 cgccggatca gctcggcgag gatgtcgcgg cgggcgcggt accaccaatg gcgcgtatcg
    23461 agctccgcca tgcgatcgta gacgacgcgt tccatcagtt cgtgaccatc aggcgaacac
    23521 ccatcggcgg ttcaatgaaa aggtgaccag cggcgtcacg aacaggatcg ggatgaccgg
    23581 ccaccaggtc ggccccccca tctgcttcac cagcagccag acgaagaact ggttggcgag
    23641 gaaccccgaa gtgttgacga tgaagaagcg cgcgctgcgg gcggcggagc ggtcgcgtga
    23701 gccatgacct ttgaagctcc agcggctgtg gagcagatag ccgaggccgg tggcgagcag
    23761 atagttgatg gtgagcgcca gcatcggatc gacgccgaaa aatgtcgccg tcacccaata
    23821 gccgccggcg accagcacgg tcaggaaccc gccggtcagc gcgaagcgca cgacctgccc
    23881 gaacaaggcc gaatgccggt cgagatcgat tacgtgcttg atattccggc ccctgcttgc
    23941 ccttcgccca ccagcgacta atgcggcccc ggatgaatgg gaagggggag cgggtggccg
    24001 gcagcggcag cttggaacgg cattggcggc tgtggttgct gatcttctgg tcggtcgccg
    24061 ccgtcctgct cgcctaccgc cagtggaacg cgatccaatg gttcgcgctc ggcgacaccg
    24121 acgacaatct gcgcatgatg caggtgcggg ggctgctggg tgggcaggat tggttcgacc
    24181 tccgccaata tcggctcgat ccgccccacg gcgccaacgt acattggtcg cggctcgcgg
    24241 acctgccgat cgcgggcgtc atgctgctcc tgaagccgtt catgaccggg gccgcggcgg
    24301 aaaaggtcgc ggtcgccttc gcgccgatga tcccgatggg gattgcgatg gcggcgacgg
    24361 ccgtcaacgt gcggcggctg gtcgcaccgg gggccttcgc gctggccgtt gcccttctgc
    24421 tctgcgccca ttcgacgcgc ggcatgtggg cgccgctgag gatcgaccat catggctggc
    24481 agctggccct gctgagcctg gtcctgctcg gcctcaccga taccaagcgc gcccgcggcg
    24541 ggctggtcgc ggggctggcg agtgccgctt cgctggcgat cgggctggaa ttgctggtct
    24601 atctcgcggc cgcgggcgcc gtcgtcgcct tgatgtggat ccgggacggc ggcgaggcgc
    24661 ggcggctggc cacctacggg gccagcctcg ctggcggcac cgccttcggc tatctggtct
    24721 tcgcctctta tgccaatcgc gcgccggtct gcgacgcgct gtcgccggta tggctgtcgg
    24781 cgatggtcgc ggccggcgcg ctcggcttcc tgttgacacg ggtaccggcg gcaagctggc
    24841 aggcgcggct ggcgctggcg gcggcggccg ggggggtgct cgcggccttc ttcgcgctgg
    24901 cctggccgca ttgcctcggc cggctcgagg gggtctcgcc ggagctttac gggatgtggc
    24961 tgggcaatgt ccgcgaggcg cggccggtct acacgcatgg ctggcaagtg atcgccaatg
    25021 tcgtcgcgct gcccgccgcc ggcctggtcg gctatgcgct catgctctgg cgggtgcggc
    25081 gggagccgcc ggcgctgatc ccgtgggcgg cgaccgcctt cctggccttc atcccctgtg
    25141 ccttgctgtt gtggcagagc cgggcgggcc cggccgccca gctgctcgcg gtgccggggg
    25201 cgacagcgct cgcctggctc ctcatccagc gggcgctcgc cgcctccaat ttgcttgtgg
    25261 ccgcggggtt ctcggtcgtt acgttttggg tggtttcggg actggccgtt cagcagctgc
    25321 cgccgctgct cgagaagccg gctaccggtc acgtccaggc ggtcaaccgg gccaacaatc
    25381 tgtgcccgac gctggcggcg ctacggccgg tggcgcgggt gccgcgcggc tatgtgctca
    25441 cccatgtcga cctcgggccg cggctggtca cggtcaccca tcatgatgcg gtggcggggc
    25501 cctatcaccg caaccaggcc gcgatcctcg atgtcatgaa gagcttccgc ggttcggtgg
    25561 actatgcgcg ctcggtgatc gagcgccgcg gcgtaggcta tgtactgatc tgccctggcc
    25621 tctcggaatc gaccgtctat gcgaaggagg cgccgaaggg cttctacgcc cagctcgccg
    25681 gcggccgggt gccggcctgg ctggaaccga tcgcgctgcc gcgcgactcg ccctaccgga
    25741 tgtggcgcgt cgtcagacgt tgatctggaa gctgacgctg atcccgtcga tcacgaactg
    25801 ggcggcgagc gcggcgagga tgacgccgag gatgcgggtg atcatcgcct cgaccttgtg
    25861 accgaggagc cgcatcagcg ggccggcggc aagcagcgcg agcagggtga gcagcaggat
    25921 cgcgccgagc gcgcctagca cgatcagcga ttcgtcgagg ccgtcgctgc gcgccatcag
    25981 cagcatgatc gacgcaatcg agccggggcc ggcgatcatc gggatggcca tcgggaagac
    26041 ggcgatatcc tcgatctcgg gggtggcgtt gatctcgctg gcgcggctct cgcggcgctg
    26101 ggtgcgcttt tcgaacacca tctcgagcgc gatcaggaac agcatgatcc caccggcgat
    26161 gcggaaggcg gcgaggctga tgcccagcgc gtcgagcaga tcctcgccga acaggccgaa
    26221 gaagagcagg atgccggccg cgatcagcac cgagcggatc gccatcatcc gccgctcggc
    26281 gggcggcgct tcgtgagtga ggctggcgaa gatcggcgcg cagccgggcg gatcgatgat
    26341 cacgaagaag gtgacgaatg cggaggcgaa cagctccatc atgaggcgcc gccctggtgg
    26401 gtccgtccgc ttcgcccgtt cgccccgagc gaagtcgagg ggggccgccg agcgaagccg
    26461 aggcccttgc tctcggctgc gcttgaaggg gtgtctcgac ttcgctcgac acgaacgggc
    26521 gtgatcatag tccctccggc gccaggtatc ccgcccgcgc gtgggcggcg acgagggtgt
    26581 tgcggaggag gacggcgatg gtcatggggc cgacgccgcc ggggacgggg gtaatggcgc
    26641 cggcggtctc gaccgcggtt tcgaattcga cgtcgccgac cagccgggtc ttgccccggc
    26701 cgatctcgat gcggttgatg ccgacatcga cgacggtggc gccgggcttc agccagtcgc
    26761 cgcggatcat ctgcggccgg ccgaccgcgg cgacgaggat gtcggcgcgc cgcaccacct
    26821 cgggcaggcc gcgggtgcgg ctgtgggcga tcgtcaccgt gcaattttcc cggagcagca
    26881 gctgcgccat cggcttgccg acgatgttgg agcgccccac caccaccgct tctagccccg
    26941 acaggtcgcc cagccggtcc ttgagcagca tcaggcagcc gagcggagtg cagggcacca
    27001 gccccggctc gccgaccgca agccggccgg cgttgacgac gtggaagccg tcgacgtcct
    27061 tggcgggatc gatggcggcg atcaccgcct ggtcgtcgat ggcggcgggt agcgggagct
    27121 ggacgaggat gccgtcgacg gcgggatcgc ggttgagccg ctcgaccagg gcgatcagct
    27181 cggcttggct ggtatgatcg ggcaggaaat gctcgaagcc caccattcca gccgcttcgg
    27241 tcgccttgcc cttggcgcgg acatagacct ggctggccgg atcctcgccg accagcacca
    27301 cggcaaggcc gggtttgcgg ccggaccgtg cgacgaagcc aggcaccgcc gcggcgaccc
    27361 ggccgcggag ccgctcggca taggccttgc cgtcgatgag gtcggccgcc atcctcaggc
    27421 gagggcgacg tcggcggcaa gcccgcgcag cagccggtcg gctatcatga tgaggatcag
    27481 caccacgaac gggctgaagt cgatgccgcc gaaatcgggc aggatcttgc ggaacggccg
    27541 gtagagcggc tccgtgatcc ggtcgagcgc gccgaggaac gaccgcacga aatcgttata
    27601 ggtgttgatc acgttgaagg cgaccagcaa actgaggatc gcctggatga tgatgatcca
    27661 ccacaggacc gtcagcagaa tgatcagcac gtcaatcagc gccaaagcca tcaaactctc
    27721 cccgcgagca ccgtcccggc atgtaggata gcgggaaggg aggggcaatg gcggccggtg
    27781 agcggtttca tcgccgcacc ggcgcgggag cccggccagg ccaagccggc gcggccttga
    27841 tgagcgggtg ccacgacccc atcaaaagga gcggcgccgg aacggaaagc cgggacggcg
    27901 cgctttggcg gctcagcgga cgggagatcg aaatgaaggg ctatgtggac gatatcgagc
    27961 gcgcgacgat cgagaacgag gatttccgcc gcgtcctcta taccggcaag aacctccagc
    28021 tggtgctgat gacgctgccg ccgggcgagg agatcggcga ggaggtgcat gaggatcgcg
    28081 accagttctt ccgcatcgag cagggccgcg gcgaagtccg gatcgacggc aaggccaatc
    28141 cggtcgagga cgatttcgcc gtgatcgtgc ccgctggcgc ccgccacaat gtcgtgaaca
    28201 ccggcgagga gccgctccgg ctctacacgc tctacggccc gcctgagcat aaggacggcg
    28261 tcgtccacaa ggacaaggcc caggccgagc gcgatcacga caatgacgaa tggaccggcg
    28321 aaacgacgga gtagcgtctc ctatttgggc cggggccagc agaggtctgc gaggcgcttg
    28381 ggcaagtcgc tggtcatctc accgtcgcgg gagccgacgc cccaggtgtt ggcaaggacc
    28441 gcgacgacga ggccgcgttc ggggtagatc atgaggccgg cctgcacgcc ggggttggcg
    28501 ccgctgatgg tgatccgtct ctcggcgccc gcatcggggg cgaagaagcc gaagctcatc
    28561 gccgacggtt cgccgccgaa ggtcggctgc gagaatagca gatcgtgctg ggccggcggc
    28621 agcaagccgg gcccaaggag ggccgccccg aactttacca gatcctcggc ggtcgagctc
    28681 atattgccgc cgcccatatt gtgggaataa tcccgcggcg gcaccgggat cggatccggg
    28741 atctccgcat aggtcttgag atcgtagaag ctgtagaggc gagcccggcc cggcggggga
    28801 gttcgggcgt cgtcgaaacg ggtcttcgaa agccgcagtg gccggaggat ctgccttcgc
    28861 acgtagcgcc tgaattcaac gccgacggag cgttcgaggg cgagggcgag aaggttgtag
    28921 ccgtagctcg aatattgata ttcagcgccg gccttggcga gcagcggatc gtccttgaac
    28981 agcggcaaga tctggtcgag ccgatcgaag cggcgggcga agagggcctc ccgctccgct
    29041 ccccagtgcc ggatgcccgc gcggtgcgcc gccaggcggc gcggcgtgac cacgagttcg
    29101 ggcttcaccg ggaattcggg cacgtagctc tggatgggat cgtcgagacg gaagctgcct
    29161 gcggcgtgtg ccttcagcaa ggcggcgccg gtgaacgcct tggtgatact ggcgaccggg
    29221 aagatggtgt tccgcgtcac cgggatcttt cgctcccgat ccgccagccc gtgagcttgc
    29281 gagaaggcga gccggccgtc gaggtggatc gccgcctgca cgccgacatt cttctgcttg
    29341 tcgtggaccg aacggatggc ggcgagcagc tccgcgtcgg tcgcccggca cgcttgcggt
    29401 gcggcaaagc ccggtccggg cgccacggcg gcgagcatcg ccgccccgct atacaggcag
    29461 ttccgcagcg ttgcgatcga tggcataata tcctccggta tgagcggtcg atggtcggct
    29521 gagcttaggg aaaggcgggt ccgccagtcc tctcgccggg ctaatggagt tctccttttt
    29581 tgctgatgga cgaggatcat ccagccggat cggcgacgct ggcggccatg gccgggcctt
    29641 tccaggtcgg cgcctggcgg gccgaaccgg cctggcaccg gatcagccgg agcggcgaga
    29701 tacgcaagct cgagccgcgg gtgatgcgtc ttctcgccgt gcttgcggcc gcgccgggcc
    29761 ggcccctgac gcgcgaacaa ttgctcgacg ccgtctggcc gaacctccac gtgaatgaag
    29821 acgcgctctc cagggccgta tcgcagctgc gccgggcctt atcggacgat ccccgcaagc
    29881 cagcctatat cgagaccgtt cacaagggcg gctattgcct tgtcgcttcg gtcggcagcg
    29941 tcgaggccgc tccggcgggc aggacggaga ggcgctcggc taccacccct aagcgaatgg
    30001 cggtcgccgc cgccgctctt ctcgcgctgc ttgccggcgg tttcctcctc accagcctcg
    30061 atccggcggg aacgtcggcc gtgaaggcgg cgctcgtccc ggtcccgctc accagcgagt
    30121 cggggcgcga gatcgatccc gccatttcgc gcgacgggcg gcgcctcgcc ttttcggcct
    30181 cgacggaagc gggctacgac cttttctggc gggccatcgg caacgaccgc tccgtccgcc
    30241 tgacccatga cggccgcttc gccgaatatc ccgcctggtc tcccggtggc gaccggatcg
    30301 cgttcctcag gagagaggcc gacggagccg caatctacat cgccaccctg gcgaccggcc
    30361 ggctggaaaa gctggttggc cttcgtgcct ggtcgttcgg cctcgattgg agccccgacg
    30421 gacgcaccat cgcctatagc gacagctctg acggcgaggc ccgaagcatc gtcctcatcg
    30481 acctcgcgtc tcgggtccgc cgcacgatca cccgcgccgc ctcgtggcgg ggcgacggac
    30541 ggccggtatt cgctcccgac gggcgccgcc tggcctttct ccgcgacgcc gggctcggcc
    30601 tgagccggat cttcatcgcc gacctggccg atcccgacag cgcgcgagcg ctgacggttc
    30661 ggccggagga gatcaaaggg ctggactggg atccggctgg cggcggcctc gtctattccg
    30721 cccggtccgg cggccgctac ggcctatggc ggatgccggt tgagggtgga cggccgcagc
    30781 cgatcccgac cgaaggcggc gacctgttca atccctccgt gtcggagcag ggcgtgttgg
    30841 tagccgaagc cgtcgagcgg gacagcgacg tctggcaggc gcgcattgac ggccgcggcg
    30901 cggcgcctct gttacgctcg acgcaagacg attatgatcc cgctcactcc ccctatcggg
    30961 gccggatcgc gttcgtttcg tccagatccg gttcccccga actctggctc gcccgatccg
    31021 acggagccca cgcccgccaa ctcacccgcc tgggcggcgc tcacgtcggc ggcccggcct
    31081 ggtcgccgga cgggcggcag atcgccttcc agtcgcaggt cgacgggttt gccgcggcct
    31141 acatcctgtc gcccggcgac cggccgccgg tgctcgtcgc cggcggcggc aagcagcatc
    31201 atgtcccgct cggatggggg cgaaccggcg gggaattgtt caccgcatcg gggtcggggt
    31261 cgagctggac ggtgcggcgg cacgatatcg cggcgaggcg ttcgaaggcg ctgaatgcgg
    31321 tccccgcccg ccttgccggg gtcgcgcccg atcggcagtc ggtctatctg atcacccatg
    31381 acggtgcgac gctgacccgg gtctccgcca gctccggaag ggccgaggaa tttccgctgc
    31441 cgggagcgtt gcgggctgcc gacgcgatgc gcgcggccgg cgccaatttc tacattttcg
    31501 tgccgagccc agggggcacc gcgatccatc ggctgaggcg ggtgggggcg ggactggagt
    31561 tcgaccgggt cgcctcggtg cccggttacg gccaggtctc ggtctcgccc gacgagaccc
    31621 agttgctctt caccagcgac cgtgagaccg cgaacgacat cgtcctgttg cggctgtgac
    31681 atcgccgatg acgtcgccca ggcaaatggc tatcgaccgg ccatggccaa gggaacccac
    31741 gacagcgcgc ccgatccgcg caacgccgac atcctcatca acgtcaacgg cgagctgaag
    31801 ccgcgggacc aggcaatggt ctcggtgttc gattccggtt tcgtgctcgg cgacggcgtc
    31861 tgggaggggc tgcgcgtcca caaggggcgg atcgccttcc tgggccggca tctcgaccgc
    31921 ctgtgggagg gcgccaaggc gatcgccatg gacatcggcc tctcccgcga ggcgctggag
    31981 cggcggctct acgaaacgct cgacgccaac ggcatggacg aaagccccgc ccaggagggg
    32041 gtccatatcc gcctgatggt cacgcgaggc attcgctcga cgccctatca ggacccgcgg
    32101 gtggtgatct cggcgccgac gatcgtcatc atccccgaat ataaggcgcc gctgccggtc
    32161 acgatcgagc gcggcatccg cctgttcacc gtccacgtcc gccgcggcga cccggccgtc
    32221 caggacccca agctcaactc ccattccaag ctcaacgaca tcaccgcctg catccaggcg
    32281 gccacggcgg gctatgacga ggcgctgatg ctcgatccgc acggcttcgt tgcgacctgc
    32341 aactcgaccc acttcttcat cgtccgcaag ggcgaggtgt ggacctcgac cggcgattat
    32401 acgctcggcg gcatcacccg ccgcaacgtc atcgagctct gccgcgagga cggcatcccc
    32461 gtcttcgaga agaacttctc gctgaccgac gtctacggcg ccgaggaggc gttcgtgacc
    32521 ggcaccttcg cgggcgtggt gccggtgcgc gagattgacg gccgtcacat cagcgaggcg
    32581 cgcgggccga tggtcgagcg cctccagcgc ctctacaagg ctcggatcaa agccgacgtg
    32641 gcgggccgct cgcggccgtg accgtccgga tcgcgatgtg gtcggggccg cgcaacatct
    32701 cgacggcgat gatgcgcagc ttcggaagcc ggtccgacac cttcgtcagc gatgagccct
    32761 tctacggcgc tttcctcaag gacagcggcg ccgatcatcc gatgcgcgag caggtgatcg
    32821 ccgccatgga taccgactgg cgctcggtgc tgcggacgct ttccggcccc gtgcccggcg
    32881 gcacggcggt ctggtaccag aagcatatgt ggcatcacat ggtcggcccg gtcggctatg
    32941 acgatttccg cggcttcacc cacgccttcc tgatccgcga gccggcgcgg atgatcgcct
    33001 cctacatcga gaagcgcgaa gccgcttcgt tcgacgattt cggcctcgac cggcaggttg
    33061 agttctttga tcgcgaagcc gaccggctcg ggcaggcgcc gccggtggtc gatgcggccg
    33121 atgtcctgcg cgacccggag cggacgctgg gcgcgctctg cacggcgctc ggcatcccgt
    33181 tccagcccgc catgctgcgc tggaagccgg gccgccggga cacggacggc atctgggcga
    33241 gccactggta caatgcggtg gagagcagca ccggcttcgt tgcgcctgag gagagggcgg
    33301 ccgccatgcc cccgaaagcg cgccgtctgg ccgagcgctg caggccctat tatgagcgtc
    33361 tcgccgggca ccggctggcc gcgccgccgg ggtccgaatc gtaaacatct ccaattgacc
    33421 cagatcggac tcgcggctat aggccgcctc gcccggggct ggtggtggct caacaagcca
    33481 gacgctgttc ccgctacggg cgaccagagc ttgaacattg ctgcacgttt gcaaaggcct
    33541 ggggggccga tgggttccca tcaaagtaat actttgatgg ggtcccgatt ggccgccggg
    33601 gcgtttgtgc attttgcgtt gcagcaaagt aacttaagga agtgaaggct tcatgccgac
    33661 gatcaaccag ctgatccgca agggtcgcga accgcagaag gccaagtcca aggtccctgc
    33721 gatggagcag aacccgcaaa agcgcggcgt ctgcacccgc gtctatacga cgacccccaa
    33781 gaagccgaac tcggcgctgc gcaaggtcgc caagatccgc ctgaccaacc agcgcgaggt
    33841 catctcctac atcccgggcg aaggccacaa cctgcaggag cactcggtcg tgctgatccg
    33901 cggcggccgt gtgcgcgacc ttcccggcgt tcgctatcac gtcctgcgcg gcgtgctcga
    33961 cacccagggt gtcaaggatc gccgccagtc ccgctccaag tacggcgcca agcgtccgaa
    34021 gtgaccctga cgggcgagag cccgcccgga gtcaccccgg caaaggccgg ggtccagagt
    34081 ggccccggtg gcgtcaggaa gtaaaatgca tcccggcccg ccgggaacat gatgagcag
//
LOCUS       DASVRK010000002         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_62605, whole genome shotgun sequence.
ACCESSION   DASVRK010000002 DASVRK010000000
VERSION     DASVRK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62605"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VF589_00165"
     CDS             <1..358
                     /locus_tag="VF589_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8256016.1"
                     /translation="VVKRQLTAAAAPAPAPLPAAAPAPGAPAAPVATSEEKAANHPGA
                     VKSPMVGTVYLSGEPGSRPFVAPGATVAAGDTLLIIEAMKVMNPITAPRAGVVREVLV
                     QDAQPVEYDQPLVIIE"
     gene            360..1709
                     /gene="accC"
                     /locus_tag="VF589_00170"
     CDS             360..1709
                     /gene="accC"
                     /locus_tag="VF589_00170"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8256017.1"
                     /translation="MAIGKVLIANRGEIALRIHRACHEMGIKTVAVHSTADTEAMHVR
                     LADEAVCIGPPPANESYLNVPNIISAAEIAHVDAIHPGYGFLSENARFAEIVELHNII
                     WIGPKPEHIRTMGDKVEAKRTAAKLGLPLVPGSNGPVEGIEEAKAVAAETGYPVLIKA
                     ASGGGGRGMKVVQAEDQLEALMMQARSEAKNAFGDETVYIEKYLGNPRHIEFQVFGDG
                     KGNAVHLGERDCSLQRRHQKVLEEAPSPVITPEERDRMGGIVAKAMAEMGYRGAGTIE
                     FLYENGEFFFIEMNTRLQVEHPVTEMITGMDLVREQIRIAEGEALSCTQDQIRFHGHA
                     IECRINAEDPATFTPSPGTVKGFHAPGGMHVRVDSGLYVGYKVPPYYDSMIAKLIVYG
                     STRERCIMRLKRALEEFVIEGMKTTIPLHKALIEDPEFRAGDYTIKWLEEWLARSSD"
     gene            complement(1990..2694)
                     /locus_tag="VF589_00175"
     CDS             complement(1990..2694)
                     /locus_tag="VF589_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8256018.1"
                     /translation="MAAKRIDRGMLKALALLLSLASVAACSPASPVIAADELVPPEAA
                     PQTVSAELPPPGAAAAPAVQPASASSFPIQGVLQPDRPLEAGDYLWEAEGAPAGPLRI
                     VVDIEARRLYAYRGGIEIGRAHIVYGHDDKPTPTGTFKILQKNADHVSNLYDAPMPYM
                     MRLTNDGVAIHGSEVDDAYATHGCVGVPEEFAALLFEQAKLGDTVFITNGWMTDTYAE
                     GATEEPAEEEYAATAA"
BASE COUNT          484 a          879 c          957 g          420 t
ORIGIN      
        1 cgtcgtcaag cgccagctga cagccgcggc ggcgccggcg ccggcgcccc tccccgccgc
       61 cgctccggcg ccgggggcgc ccgccgcgcc ggtcgccacg tccgaggaga aagccgccaa
      121 ccatccgggc gcggtcaaat cgccgatggt cggcaccgtc tacctgtcgg gcgaacccgg
      181 ctccaggccg ttcgtcgccc ccggcgcgac ggtcgccgcc ggcgacacgt tgctgatcat
      241 cgaggcgatg aaagtgatga acccgatcac cgcgccacgc gccggcgtgg tccgcgaagt
      301 cctcgtccag gacgcgcagc cggtcgaata tgaccagccg ctcgtgatca tcgaataaga
      361 tggcgatcgg caaggtcctg atcgccaatc gcggcgagat cgcgctgcgc atccaccgcg
      421 cctgccacga aatggggatc aagacggtgg cggtgcattc gaccgccgac accgaggcga
      481 tgcacgtccg gctggccgac gaagcggtct gcatcggccc gccgccggcc aacgaatcct
      541 atctgaatgt ccccaacatc atctcggcgg ccgagatcgc acatgtcgac gcgatccacc
      601 ccggctacgg cttcctcagc gagaatgcgc gcttcgccga aatcgtcgaa ctgcacaaca
      661 tcatctggat cggtcccaag cccgagcata tccggacgat gggcgacaag gtcgaggcca
      721 agcgcaccgc cgccaagctc ggcctgccgc tggtccccgg ctcgaacggc ccggtcgagg
      781 ggatcgagga agccaaggcg gtggcggccg agaccggcta tccggtgctg atcaaggcgg
      841 cgtcgggcgg cggcggccgc ggcatgaagg tggttcaagc cgaagaccag ctcgaagcct
      901 tgatgatgca ggcgcgctcg gaagcgaaga acgcgtttgg cgacgagacc gtctatatcg
      961 agaaatatct cggcaacccg cgccatatcg aattccaggt gttcggcgac ggcaagggca
     1021 atgccgtcca cctgggtgag cgcgactgct cgctgcagcg gcgccaccag aaggtgctgg
     1081 aggaggcccc ctcccccgtc atcacgccgg aagagcgcga ccggatgggc ggcatcgtcg
     1141 ccaaggcgat ggccgagatg ggctaccgcg gcgccggcac gatcgagttc ctctacgaga
     1201 atggcgaatt cttcttcatc gagatgaaca cgcggctcca ggtcgagcat ccggtgacgg
     1261 agatgatcac cggcatggac ctggtgcgcg agcagatccg gatcgccgag ggcgaggcgc
     1321 tgagctgcac ccaggaccag atccgcttcc acggccatgc catcgaatgc cggatcaacg
     1381 ccgaggaccc ggcgaccttc acgccgtcgc ccgggacggt caaaggcttc cacgcgccgg
     1441 gcgggatgca cgtgcgcgtc gatagcggcc tgtatgtcgg ctataaggtg ccgccttatt
     1501 atgactcgat gatcgccaag ctgatcgtct acggctcgac ccgcgaacgc tgcatcatgc
     1561 ggctgaagcg ggcgctcgag gaattcgtga tcgagggcat gaagacgacc atcccgctcc
     1621 acaaagcgct gatcgaggat cccgaattca gggcggggga ctatacgatc aagtggctgg
     1681 aggagtggct ggcgcggtcg agcgattagg gggatcgatc gaggctggat cgtgaccccg
     1741 gacttgatcc ggggtctgcc ttcttcaggt cgcggctgga aggaagaagg cgggtgccgg
     1801 atcaagtccg gcatgacgaa gggggggatc aagcaccaac ccgctcatcc tgaggagccg
     1861 ctgagcgaag tcgaaccggc gtctcgaagg acgcacgggt gcttcgagat gggccttcgc
     1921 cggggctcag tccctcctca gcatgagcgg ggagggtccg atcttgcagg tgacttcatc
     1981 cacgcctgct cacgccgcgg tggcggcata ttcctcttcg gcgggctctt cggtggcgcc
     2041 ttcggcatag gtgtcggtca tccagccgtt ggtgatgaag acggtgtcgc cgagcttggc
     2101 ctgctcgaac agcagggcgg cgaattcttc ggggacgccg acgcagccgt gggtggcata
     2161 agcgtcgtcg acctcgctgc cgtggatcgc gacgccgtcg ttggtcagcc gcatcatgta
     2221 gggcatcggc gcgtcgtaga ggttcgagac gtggtcggca ttcttctgca ggatcttgaa
     2281 agtgccggtc ggggtcggct tgtcgtcatg gccgtagacg atgtgcgcgc ggccgatctc
     2341 gatgccgccg cgataggcgt agaggcggcg cgcctcgata tcgacgacga tgcggagcgg
     2401 cccggcgggc gcgccttcag cttcccagag atagtcgccg gcctcgagcg ggcggtcggg
     2461 ctggagcacg ccctggatcg ggaagctgga ggcggaggcg ggctggaccg cgggggcggc
     2521 ggccgctccc gggggcggaa gctcggcgga gacggtttgg ggcgcggcct cgggcggcac
     2581 cagctcgtcc gccgcgatca ccggcgaggc gggcgaacag gccgcgaccg aagcgagcga
     2641 gagcaaaagc gccagcgcct tcagcatgcc ccgatcgatc cgctttgccg ccatccgtat
     2701 ctctccgaat ccagttgagg gccgatatcg cgtccgcggc
//
LOCUS       DASVRK010000003         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_187436, whole genome shotgun sequence.
ACCESSION   DASVRK010000003 DASVRK010000000
VERSION     DASVRK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187436"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VF589_00180"
     CDS             <1..548
                     /locus_tag="VF589_00180"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX8256019.1"
                     /translation="VAAYQQLAEAVDCPLHLGITEAGGLRGGTVKSAIGIGSLLWFGI
                     GDTIRVSLSAEPEEEVRAGFEILKALGIRNRGVRVVSCPSCARQGFDVIRTVQTLEER
                     LAHIRTPLSLSVLGCVVNGPGEARETDIGITGGGNGKHMVYLSGVTDHHVQDDAMVDH
                     IVRLVEAKAAEIEAAGARAAA"
     gene            617..1087
                     /locus_tag="VF589_00185"
     CDS             617..1087
                     /locus_tag="VF589_00185"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX8256020.1"
                     /translation="MLKPFILVIAAGATIGLMLPSSRPAAAPVAAAPGGEAVETVLER
                     EENGHFYVHAYVNGELVRFLVDTGASSVALTMADAERLGIKFKQSQFEVVGTGASGPV
                     RGKDVMIDSVEIDGKKVAPVQGAVIEGLDISLRGQAYLSRISGVEMSGGHMILR"
     gene            1121..2026
                     /locus_tag="VF589_00190"
     CDS             1121..2026
                     /locus_tag="VF589_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8256021.1"
                     /translation="MASTSKTQGTLIAFGVATLGIACFSIMDALMKGLAIALGTYNAL
                     LWRVLALVAMAGLLFLVRRSPWPKRPAMRLHFIRGSMSSMMALLFFWGLARVPLAQGV
                     ALAFVAPLIALYLAALLLKEKIERRAIYASLVGFAGVLVILSAQAEADLGPEAFRGAL
                     AILASAGLYAWNIILMRQQALAAGPVEIAFFTTLFMGSYFLAAAPFLAEAPPPDRLAE
                     IVGAAFLAFLSILLLSWAYARAEAQYLAPVEYTAFVWAVLLGWMVFAEPVRPLTILGA
                     AMIVAGCIIAARRGAPRVADPVAGP"
     gene            2026..2340
                     /locus_tag="VF589_00195"
     CDS             2026..2340
                     /locus_tag="VF589_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX8256022.1"
                     /translation="MAWALLLLGGLFEVGFTTCLRFVEGFRNLPWTLGFLASVTLSMG
                     LLEYAARTIPMGTAYVVWGGIGAIGTVAVGMIWFGEPTTTLRILFILGIVGCIAGLRL
                     IA"
     gene            2337..2702
                     /locus_tag="VF589_00200"
     CDS             2337..2702
                     /locus_tag="VF589_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX8256023.1"
                     /translation="MSVDEGLYAWIEEALEPIGRLSMRRMMGGATLYLDGTIFAIMDD
                     GELWFKADAETDAVWDEAECERFSVTFKDGRVDTMNYRRAPAGVYDDAEAMQRWAALA
                     IAAGLRGVAKKKPKKKKPV"
     gene            complement(2699..4252)
                     /locus_tag="VF589_00205"
     CDS             complement(2699..4252)
                     /locus_tag="VF589_00205"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8256024.1"
                     /translation="MSLRPLLLGAALALGACAAGPARDGHALVLDAASAESRTRSAIE
                     RIEALNPRLNAVIGVEPAAIAEARALDRGWAARGPLFGMPILVKDNIETRGTLPTTAG
                     SLALKDNVTGRDAPLVARLRAAGAVIVAKTNLSEWANMRSSDSISGWSPVGGQTRNPH
                     ALDRNACGSSTGSAVAVAAGMAPAAIGTETDGSITCPAAVSGIVGFKPSVGLVSRTHI
                     IPISRSQDTPGPMTRTVRDAALVLAAMAGTDPADRATREGDGRRTDYAAALSDRSLQG
                     KRIGVMRFATGFGTEAAFEEALATLRREGATLVDIDKFEGREEIGKAEFAVLLAEFKD
                     GINAYLATTPPAVRTRTLADLIAFNQANAATEMPLFGQDVFEQAEKTKGLDEPGYRTA
                     RSTSLRMAGPEGIDRLLREHKLDALVGPTMSPAWLIDAVHGDQTPGGGGGSLAAVAGY
                     PHLTVPMGIVKGLPVGLSFIGPKWADALILSLGYDYEQASRKRVEPALIPSVEQSDDV
                     ARAFEPAAR"
     gene            complement(4249..>4613)
                     /locus_tag="VF589_00210"
     CDS             complement(4249..>4613)
                     /locus_tag="VF589_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX8256025.1"
                     /translation="NAYDASAMKRRKALDLTHSLGLSIGKSGAVSGVVWDGPAFRQGV
                     TGNASIVAVNGREYSDDEIKGAIAGARGSNQPIRLLLKEGSRYREVAITYNQGLRWPH
                     LERAARTASLDRLLAPLR"
BASE COUNT          647 a         1578 c         1591 g          797 t
ORIGIN      
        1 ccgtcgccgc ctatcagcag cttgccgaag cggtcgattg cccgctccac ctcggcatca
       61 ccgaggccgg cggccttcgc ggcggcaccg tcaagagcgc gatcgggatc ggctcgctgc
      121 tctggttcgg gattggcgac acgatccgcg tctcgctttc ggccgagccg gaggaagagg
      181 tacgcgccgg cttcgagata ttgaaggcgc tcggcattcg caaccgcggg gtgcgcgtcg
      241 tctcctgccc gtcctgcgcc cgccagggct tcgacgtgat ccggacggtg cagacgctcg
      301 aggagcggct ggcgcatatc cgcacgccgc tgtcgctgtc ggtgctcggc tgcgtcgtca
      361 acggccccgg cgaggcgcgc gagacggaca tcggcatcac cggcggcggc aacggcaagc
      421 atatggtcta tctctccggc gtcaccgacc accatgtcca ggacgacgcg atggtcgatc
      481 atatcgtcag gctggtggag gccaaggcgg ccgagatcga agcggccggc gcacgcgcgg
      541 ccgcttgagc gcgcttcact tttcatcaac catatccggc aacggggctg aagctgctca
      601 gcggctaggc cggggcatgc tgaagccgtt catcctcgtc atcgccgccg gcgctaccat
      661 cggattgatg ctgccctcga gccgcccggc ggctgcgccc gtggcggccg cgcccggggg
      721 agaggcggtg gaaaccgttc tggagcgcga ggagaacggc cacttctacg tccatgctta
      781 cgtcaatggc gagctcgtcc gtttcctggt cgataccggc gccagcagcg tggcgctcac
      841 catggccgat gccgagcggc tcggcatcaa gttcaagcaa agccagttcg aagtggtcgg
      901 aaccggtgcc tccggcccgg tccggggcaa ggatgtcatg atcgacagcg tcgagatcga
      961 cggcaagaag gtcgcgcccg tgcagggcgc cgtaatcgag gggctggaca tctcgctgcg
     1021 cggccaggcc tatctgtcgc ggatcagcgg cgtcgaaatg agcggcgggc atatgatcct
     1081 gcgctagcgc aggcgggatc ggggcggcta tgggccccgc atggcctcga cctccaaaac
     1141 ccaaggcacc ctgatcgcgt tcggcgtggc cacgctcggc atcgcctgct tttcgatcat
     1201 ggacgcgctg atgaaggggc tggcgatcgc gctcggcacc tacaatgcct tgctgtggcg
     1261 ggtgctggcg ctggtcgcca tggccgggct gctcttcttg gtgcggcgga gcccctggcc
     1321 caaacggccg gcgatgcggc tgcacttcat ccgcggcagc atgtcttcga tgatggcttt
     1381 gctgttcttc tggggtctgg cgcgggtgcc gctggcgcag ggggtggcgc tcgccttcgt
     1441 cgcgccgctg atcgcgctct acctcgcggc tttgctcctc aaggagaaga tcgagcggcg
     1501 ggcgatatac gcctcgctgg tcggctttgc cggcgttttg gtgatcctct cggcccaggc
     1561 cgaagccgat ctcggccccg aggcgttccg cggcgcgctg gcgatcctcg cttccgcggg
     1621 cctctatgcc tggaacatca tcctgatgcg ccagcaggcg ctggccgcgg gtccggtcga
     1681 gatcgccttc ttcaccacct tgttcatggg ctcctatttc ctggcggcgg cgccgttcct
     1741 cgccgaagcg cctccgcccg accggctggc ggagatcgtc ggcgcggcct tcctcgcctt
     1801 tttgtcgatc ctgctcctgt catgggccta tgcccgggcc gaggcgcaat atctggcgcc
     1861 ggtcgaatat accgccttcg tctgggcggt gctgctcggc tggatggtct tcgccgagcc
     1921 ggtgcggccg ctcaccattt tgggcgcggc catgatcgtc gccggctgca tcatcgccgc
     1981 ccgacgcggg gctccccgag tggccgatcc ggtggcgggt ccctgatggc ctgggcgctg
     2041 ctcctcctcg gcggcctgtt cgaagtgggc ttcaccacct gccttcgctt cgtcgagggc
     2101 ttccgcaacc tgccctggac gctcggcttc ctggcctcgg tgacgctcag catgggcctg
     2161 ctcgaatatg ccgcccgcac catccctatg ggcaccgctt atgtggtctg gggcggcatc
     2221 ggcgcgatcg gcacggtggc ggtgggcatg atatggttcg gcgagcccac cacgaccttg
     2281 cgcatcctct tcatcctcgg gatcgtcggc tgcatcgccg gcttaaggct gatcgcgtga
     2341 gcgtggacga aggcctctac gcatggatcg aagaggcgct cgagccgatc ggaaggctca
     2401 gcatgcgcag gatgatgggc ggagccacgc tctaccttga tggcactatc ttcgcgatca
     2461 tggacgatgg cgaattgtgg ttcaaagccg atgcggagac cgatgcggtc tgggatgagg
     2521 cggagtgcga gcgcttttcc gtcaccttca aggacggccg cgtcgacacc atgaactacc
     2581 ggcgcgcgcc ggccggcgtc tatgacgatg ccgaggcgat gcagcgctgg gccgccctcg
     2641 cgatcgcggc aggcctgcgc ggagtcgcca agaagaagcc gaagaagaag aagccggtct
     2701 aacgcgccgc gggttcaaaa gcgcgagcga catcgtcgct ctgctcgacc gacgggatca
     2761 gcgcgggctc gacccgcttt cgcgacgcct gctcgtaatc gtagccgagc gagaggatca
     2821 gcgcgtccgc ccattttggt ccgatgaagc tgagcccgac gggaagcccc ttcacaatcc
     2881 ccatcggcac cgtcagatgc gggtagccgg cgaccgcggc caggctgccg ccgccgccgc
     2941 cgggggtctg gtcgccgtgg acggcatcga tcagccatgc gggcgacatg gtcggcccga
     3001 ccagcgcgtc gagcttgtgc tcgcgcagca ggcggtcgat cccttccggc ccggccatac
     3061 gcaaggaggt cgagcgcgcc gtccgatagc cgggctcgtc cagccccttg gttttctcgg
     3121 cctgctcgaa gacgtcctgc ccgaacagag gcatctccgt ggccgcattt gcctggttga
     3181 aggcgatcag gtcggcaagc gtccgcgtcc gcaccgccgg cggagtggtg gcgagatagg
     3241 cgttgatgcc gtccttgaac tccgcgagca gcaccgcgaa ctcggccttg ccgatctcct
     3301 cccggccttc gaacttgtcg atgtcgacga gcgtagcgcc ctcgcggcgc agcgtcgcca
     3361 gagcttcctc gaacgcggcc tccgttccga agccggtggc gaagcgcatc acgccgatcc
     3421 tcttgccctg cagcgaccgg tcggacagcg cggcggcata atcggtgcgg cgcccgtcgc
     3481 cctcccgcgt cgcccggtcg gccgggtcgg tcccggccat cgccgcaagc accagagcgg
     3541 cgtcccggac ggtccgggtc atcgggcccg gcgtgtcctg gctgcggctg atcgggataa
     3601 tgtgggtgcg gctgacgaga ccgacgctcg gcttgaagcc gacgatcccg ctcaccgccg
     3661 ccgggcaggt gatcgagccg tcggtctcgg tgccgatcgc cgccggcgcc atgcccgcgg
     3721 cgacggcgac cgcgctgccg gtgctcgatc cgcaggcatt gcggtcgagc gcgtgcgggt
     3781 tgcgggtctg gccgccgacc gggctccagc cggagatcga atccgacgag cgcatattgg
     3841 cccattccga taaattggtc ttggcgacga tcaccgcgcc cgccgcgcgc agccgcgcca
     3901 ccaacggcgc gtcgcggccg gtaacattgt ccttgagcgc gaggctgccc gccgtggtgg
     3961 gaagcgtgcc gcgcgtctcg atattgtcct tgaccaggat tggcatcccg aacagcggcc
     4021 cgcgcgcggc ccatccgcgg tcgagcgccc gagcttccgc gatcgccgcc ggctccaccc
     4081 cgatgacggc gttgaggcgc gggttcagcg cctcgatccg ctcgatcgcg ctgcgggtgc
     4141 gcgattcggc gctggcggca tcgaggacca gcgcatgtcc gtcgcgcgcc ggtccggccg
     4201 cgcaggcgcc gagagcgagc gccgccccca gcagcagggg gcgcaagctc accgcagcgg
     4261 cgccagcagc cggtcgagcg aggcggtccg cgctgcccgt tcgagatgcg gccagcgcag
     4321 cccctggtta taggtgatgg cgacttcgcg gtagcggctg ccttccttga gcagcagccg
     4381 gatcggctgg ttcgacccgc gggcgccggc gatcgcgccc ttgatctcgt cgtccgaata
     4441 ttcgcgcccg ttgaccgcga cgatgctggc attgccggtg acgccctggc ggaaggcggg
     4501 gccgtcccac accacgccgc tcaccgcccc gcttttgccg atcgacaggc cgagcgaatg
     4561 ggtgaggtcg agcgccttgc gccgtttcat cgccgacgcg tcataggcat tgg
//
LOCUS       DASVRK010000004        12042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_187580, whole genome shotgun sequence.
ACCESSION   DASVRK010000004 DASVRK010000000
VERSION     DASVRK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187580"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VF589_00215"
     CDS             <1..716
                     /locus_tag="VF589_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX8256026.1"
                     /translation="PEALPALAEELAERLAGIEAGDAGLAAMEKALRTSRLAYDEEAA
                     RLSLLRAEAGARLDLAVARELAPLKLDAARFRTIVEAQNEAQWSAAGRDRVEFEVSTN
                     PGAAFAPLTRIASGGELSRFILALKVALAERGGATTMIFDEIDRGVGGAVASAVGERL
                     HRLAAEAQVLVVTHSPQVAARGSRHLLIEKSHEGLVTRTSVHALDEAKRREEIARMLS
                     GAQVTDEARAQASRLLEAA"
     gene            713..1273
                     /locus_tag="VF589_00220"
     CDS             713..1273
                     /locus_tag="VF589_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256027.1"
                     /translation="MIAGLTVLLLSALAPPSGDAARCRLTAADKQANARLSFNDFDQQ
                     GTLPSSARKLGEAGCWEQAAEATADYLIHGPVPSPGEQRVLLFHLGQELAFAGEEERA
                     AGFIAATRRPPDPMPPKTDQLNWNDYVIGTWAFLMKDRPALIAARDAVLATAGEGNRI
                     NGALLAAMERCFDKPYEVAYDPDCGR"
     gene            1285..3402
                     /gene="ligA"
                     /locus_tag="VF589_00225"
     CDS             1285..3402
                     /gene="ligA"
                     /locus_tag="VF589_00225"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX8256028.1"
                     /translation="MTSDDAEIAAELERLAGEIARHNRLYHGEDAPEITDADYDALVR
                     RNAEIEARYPQLVRDDSPSRQVGAAPAAHLSKVRHALPMLSLDNAFADEEVADFIARV
                     RRFLKLDPGEPVALTAEPKIDGLSCSLRYEAGLLVQAATRGDGATGEIVTDNVRTIQD
                     VPQRIEGAPELFEIRGEVYMSKGDFAALNARLLAEADDPAKARQFANPRNAAAGSLRQ
                     KDPAITAARPLRFYAHGWGDVSGLPADSQLGVMRAIQSWGVPIAEDMERFDDLGALLA
                     HYRAIEAKRADLPFDIDGVVYKVDRLDWQERLGFVSRAPRWALAHKFPAEKAETILEA
                     IDIQVGRTGKLTPVARLTPVSVGGVTVTNATLHNKDEIERLGVRIGDRVRIQRAGDVI
                     PQVLENLTPAAERPAYAFPDHCPECGSEAVREEGEVDVRCTGGLICPAQRVERLRHFV
                     SRHALDIEGLGITHIEAFFRDGLLQSPADIFRLTKEKLLARERWAEISAANLIEAIDA
                     RRRPPLDRFLFALGIRHVGEVTARDLARRYRSWAAFRAMIEAALKVRAEMAPALGEPE
                     RKFFQRRDKAVVAAVETPGIGPEVANALLDFFEEPHNREVLDDLFAAGVEPQDVVWET
                     KESPVTGKTLVFTGSLETLSRDEAKAQAEALGAKVAGSVSAKTDLVVAGPGAGSKLKK
                     AAELGIDVIDEAAWNDLVAAAGG"
     gene            3496..5337
                     /locus_tag="VF589_00230"
     CDS             3496..5337
                     /locus_tag="VF589_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256029.1"
                     /translation="MASADERILAVLFHAFTSGPSKLEGVARIVREAYPQAALEIHCP
                     ALPLSLFSTADPAQVASDQADEIGQLCADAERAGSPFTRIVFVGHSIGSLLARKVYII
                     ACGETDRAPFEEAVRETGPRPWAHLVDRIILLAGMNRGWRLSHHLNPLQAAGWWLGTV
                     LGRLWERITGGKVLILGMRVGSEFITQLRVQWLRMRQAAQRDENRSGNAMTIQLLGSI
                     DDMVSPRDNVDLVAGSDFYYLDVPYSGHASIIEVDDRTEPPGAGSTAGAWRSEAIQRA
                     LASSKADLAPLQVLPADLDAVPAQHPDVTDVVFVIHGIRDAGYWTYKVARKVREAAGD
                     KAAFIATETSSYGYFPMLPFLFSFRRARNVEWLMDEYAAAVALYPHAARFHFIGHSNG
                     TYCLVEALRRYACCHFSRVVLAGAVVRSRLDWSAFLRDENRPDQRRRRHGSVEQVLNF
                     VATGDLVVAAFPRLFETFLPIQRLGGAGHIGFLDAPNAVKNVEYVKGGHGAGVAEPWW
                     PSIARFIHTGSVEPFPPGLKLDRQAWLARLLGVLPLLGWLVAVGIAVLLGWGVYLAAE
                     WVSALLVAKGGPGANALLRLLSPAADWVVPVTMTLYCLAVIKLLRWV"
     gene            complement(5551..6471)
                     /locus_tag="VF589_00235"
     CDS             complement(5551..6471)
                     /locus_tag="VF589_00235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX8256030.1"
                     /translation="MTSWKVTLPCTKAEAEALQGEIGPLALLDPPPVLMTSEADPARP
                     DDWRLDAYFEEAPSAATVAMLKALAPAAAAAEPRVEQLADADWVTLSQQGLEPIQAGR
                     FFVHTPAHRDQIPADAIPLEIDAGRAFGTGQHETTTGCLVALDRLKGTGAAVSNILDL
                     GTGTGLLAFAALKLWPAAKVAASDIDPVSIEVAEENAAINRLPLGRARGQVELAVAAG
                     LDHMRLRARAPYDLLIANILAGPLIELAPAVAAAIAPGGRLILAGLLDRQADDVAASY
                     RRRGLMLAFRVDNGEWPTLVMRKRRATGWR"
     gene            complement(6544..7248)
                     /locus_tag="VF589_00240"
     CDS             complement(6544..7248)
                     /locus_tag="VF589_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8256031.1"
                     /translation="MTQLNILVTGSSRGIGKSILAALDQHKAVGHSTRGGEGLVAADL
                     SEPGAAERLWGEALERLGGSIDVLVNNAGIFEAAPIESPAADWSAAWERTMRINLTAS
                     AELCRLAVLHFRGNGGGRIVNVSSRAAYRGDSPQHWHYAASKAGMIGMTKSIARGYAG
                     ENILAFAVCPGFTMTGMAEDYLESRGGDKLLADIPLGRVASAEEVAETVRWLAVDAPA
                     SATGAVIDVNGASYVR"
     gene            complement(7399..8739)
                     /locus_tag="VF589_00245"
     CDS             complement(7399..8739)
                     /locus_tag="VF589_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8256032.1"
                     /translation="MATAEQGRVRIEDRAGLFDPDGSLSELGREIWALIEPERDEIAL
                     AFADRYVRSIGAGALTGAASDAASAMAARLLPYVEQKFRGLTGQAWVDRVGELAEVAA
                     GEGPLTAIISGISAATARIQDILVRRLAGDPAGLARLVRALTQATLLEVEIFSAHYDG
                     IRSRIERESRSARGAEFNHEVLHAVGRAASESSSLRAQAADASAAARGMLGKTSEVAA
                     AAEQSAIAMREAAQTAAGLIQAIEDARSEVEVAAGIATRAGDKASKAVLVSQALSAHV
                     EAIESILGLIRDIAGQTNLLALNATIEAARAGDAGRGFAVVAQEVKSLASQTARATDD
                     ITAKISAIQQATRQTVEANGSIRETVAEVQSSADRIRRAMEVQAQTVTMITAAVDETA
                     LAADSMSSTIAAIRADTETVVTEIDEVERNFGEVDEQMARFTAATSHFVESFAA"
     gene            complement(8840..10234)
                     /gene="lpdA"
                     /locus_tag="VF589_00250"
     CDS             complement(8840..10234)
                     /gene="lpdA"
                     /locus_tag="VF589_00250"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8256033.1"
                     /translation="MADTYDLIVLGSGPGGYVAAIRAAQLGMKVAIVEREKLGGICLN
                     WGCIPTKALLRTSEINHYLTHAASYGLNAEKVSFDLAKIVDRSRKVAGQLNAGVKGLM
                     KKNKITVVEGEGALAGKGILKVGDPGSGSGAGKTLQAKHIIVATGARARDLPFAKTDG
                     ERIWTYRHAMVPKVMPTKLLVIGSGAIGVEFASFYSDMGAEVTIVEMLDRILPVEDGD
                     ISAFVHKALTKQGMKIHVSSGVEKLETGATGVRATLKGGATEEFSHCIVAIGIVPNTE
                     NIGLEALGVKTERGHIVTDGYGRTNVEGVYAIGDVTGPPWLAHKASHEGVVCVEAIAG
                     NNPHPFETWNIPGCTYSRPQVASVGLTEAKAREEGRQVKVGKFPFIGNGKAIALGEAE
                     GFVKTVFDAATGELLGAHMVGAEVTELIQGYAVARQLETTEADLMHTIFPHPTLSEMM
                     HESVLDAYGRAVHY"
     gene            complement(10242..10559)
                     /locus_tag="VF589_00255"
     CDS             complement(10242..10559)
                     /locus_tag="VF589_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256034.1"
                     /translation="MTLAWEARIQLMSAALMWLLAAAALAAIVIVNGLVLTSPPVSSD
                     RSAIVFGYDLALLVALGFFFKSGRSPGAKILLALVLLPAAVIGAVTVKEQLAAEASTR
                     IIR"
     gene            complement(10556..11950)
                     /locus_tag="VF589_00260"
     CDS             complement(10556..11950)
                     /locus_tag="VF589_00260"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEX8256035.1"
                     /translation="MLLREPAGERGGFDAVAGGRRRRRATPGPSGNCWRGGLRLGGGD
                     LLAEIETDKATMEFEAVDEGIIAKILVAEGTDEVKVGTVIALIAQEGEDAGAAAAAPT
                     AAPEPVAKGGGETGAGQPATPTPTPTPTPEVKGYGASPAEDEKLAQPQPQPKADTGDR
                     IKASPLARRLAEQKGLDLATLTGSGPGGRIVKADIDAASGKAAPVAAAPAAAPAQAAA
                     PAPAAAPAPAPAHGVPDIPHDAVKLSNIRKTIARRLTESKQQVPHIYLTVDVRLDALM
                     KLRAELNASLESRGVKLSVNDFMIKALAAALREVPECNVQYTGAELVTFKRADISVAV
                     SIPAGLITPIIVDAGSRSLSAISTEMKDLAARAREGKLQPHEYQGGTASLSNMGMYGI
                     ANFQAVINPPQGMIMAIGAGEKRPHVIDDSLQIATMMSATGSFDHRAIDGADGARLMQ
                     AFKRLVENPLGMIA"
     gene            11954..>12042
                     /locus_tag="VF589_00265"
     CDS             11954..>12042
                     /locus_tag="VF589_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX8256036.1"
                     /translation="MDLSALTGSGPAGRIVKADVDAAAGKPKAA"
BASE COUNT         1750 a         4023 c         4289 g         1980 t
ORIGIN      
        1 atccggaggc gctgccggcg ctggcggagg agctggccga gcggctggcg gggatcgagg
       61 ccggcgatgc cggactggcg gcgatggaaa aggcgctgcg gacgagccgg ctcgcttatg
      121 acgaggaagc ggcgcggctg agcctgcttc gcgccgaggc gggggcgcgg ctcgacctgg
      181 cggtggcgcg ggagctggcg ccgctgaagc tcgacgccgc ccgcttccgc accatcgtcg
      241 aggcgcagaa cgaggcgcag tggagcgcgg ccggtcgcga ccgggtggag ttcgaagtgt
      301 cgaccaatcc gggcgccgcc ttcgcgccgc tgacccggat cgccagcggc ggcgaattgt
      361 cgcgcttcat ccttgcgctc aaggtggcgc tggccgagcg cggcggcgcg accacgatga
      421 tcttcgacga gattgaccgc ggcgtcggcg gggcggtggc gagcgcggtc ggcgagcggc
      481 tccaccggct cgccgccgag gcgcaggtgc tggtcgtcac ccacagcccg caggtggcgg
      541 cgcgcggcag ccgacacttg ctcatcgaga agagccatga ggggctggtg acccgtacgt
      601 ccgtccatgc gcttgacgaa gcgaaacggc gcgaagagat tgcacgcatg ctctcgggcg
      661 cgcaggtaac ggacgaggcc cgagcgcagg cctcccgcct tttggaggcc gcatgatcgc
      721 tgggctgacc gtcctcctcc tctcggctct cgcgcctccc tccggcgacg ccgcgcggtg
      781 ccggctgacc gctgccgaca agcaggccaa tgcccgcctc agcttcaacg atttcgatca
      841 gcaagggaca ttgccgagca gcgcccgcaa gctcggcgag gccgggtgct gggagcaagc
      901 ggccgaggcc accgccgatt acttaatcca cggtccggtc ccgtcgccgg gcgaacaacg
      961 cgtgctgctc ttccacctcg gccaggagct ggccttcgcc ggcgaagagg agcgggccgc
     1021 cggcttcata gccgcgacgc ggcgtcctcc cgacccgatg ccgcccaaga ccgaccagct
     1081 gaactggaac gattatgtga ttggaacctg ggctttcctg atgaaggacc ggcccgcttt
     1141 gatagccgcc cgggacgcgg tgctggcgac cgcgggtgag gggaaccgca tcaacggcgc
     1201 ccttctcgcc gccatggaac gctgtttcga caagccttac gaggtggctt acgatcctga
     1261 ttgcgggcgc tgaggggccg gcggatgacc tcggacgacg ccgaaattgc tgccgagctc
     1321 gagcggctgg cgggggaaat tgcccgccac aaccggcttt accatggcga ggacgcgccg
     1381 gagataacgg acgcggacta tgacgcgctg gtccgccgca acgcggagat cgaggcgcga
     1441 tatccgcagc tcgtgcgcga tgacagccct tcgcggcagg tgggggcggc gccggcggcg
     1501 cacctgtcca aggtgcggca cgcgctgccg atgctcagcc tcgacaatgc cttcgccgac
     1561 gaggaagtgg cggacttcat cgcgcgggtg cggcggttcc tgaagctcga cccgggcgag
     1621 cccgtggcgc tcaccgccga gcccaagatc gacgggctga gctgctcgct tcgctacgag
     1681 gcggggctgc tcgtccaggc cgcgacgcgc ggcgacggcg ccaccgggga gatcgtcacc
     1741 gataacgtgc gcacgatcca agacgtgccg cagcggatcg agggtgcgcc ggagctgttc
     1801 gagatacgcg gcgaagtcta tatgtcgaag ggcgacttcg cggccttgaa cgcgcgcctg
     1861 ctcgccgaag cggacgaccc cgccaaggcg cggcaattcg ccaacccgcg caacgccgcc
     1921 gccggttcgc tgcgccagaa ggaccccgct atcactgccg cgcggccgct gcgcttctac
     1981 gcccatggct ggggcgatgt ctccggcctt cccgccgaca gccagctggg cgtgatgcgg
     2041 gcgatccaaa gctggggcgt cccgatcgcg gaggatatgg agcggttcga cgatctgggc
     2101 gcgcttctcg cccattaccg ggcgatcgag gccaagcggg ccgacctgcc gttcgatatc
     2161 gacggcgtcg tctacaaggt cgaccggctc gactggcagg agcgtttggg cttcgtcagc
     2221 cgcgcgccgc gctgggcgct ggcccataaa ttcccggccg agaaggccga gacaatattg
     2281 gaggcgatcg acatccaggt cggccgcacc ggcaagctga cgccggtggc gcggctgacg
     2341 ccggtcagcg tcggcggcgt caccgtcacc aacgccaccc tccacaacaa ggacgagatc
     2401 gagcggctcg gggtccgcat cggcgaccgg gtccgcatcc agcgcgccgg cgacgtgatc
     2461 ccgcaagtgc tggagaatct gacgcccgcg gcggagcggc cggcttacgc cttccccgac
     2521 cattgcccgg aatgcggatc ggaagcggtg cgggaggaag gcgaagtcga cgtgcgctgc
     2581 accggcggcc tgatctgccc cgcccagcgg gtcgagcggc tgcgccattt cgtgtcgcgc
     2641 cacgcgcttg acatcgaggg gctcggcatc acccatatcg aggccttctt ccgcgatggc
     2701 ctgctccagt cgccggccga catcttccgc ctcaccaagg agaagctgct cgcccgcgag
     2761 cgctgggccg agatatcggc ggccaatttg atcgaggcga tcgatgcgcg gcggcggccg
     2821 ccgctcgacc gcttcctgtt cgcgctcggc atccgccatg tcggcgaggt caccgcccgc
     2881 gacctcgccc gccgctaccg gagttgggcc gctttccggg cgatgatcga ggcggcgctg
     2941 aaggtccggg cggagatggc gcccgcgctg ggcgagcccg agcgcaagtt cttccagcgc
     3001 cgggacaagg cggtggtggc ggcggtggaa acgcccggca tcggcccgga agtcgccaat
     3061 gccctgctcg acttcttcga ggagccgcac aatcgcgagg tcctcgacga cctcttcgcg
     3121 gcgggggtcg agccgcagga tgtggtttgg gagacgaagg aatcgccggt caccggcaag
     3181 actttggtgt tcaccggcag cctcgagacg ctgtcgcgcg acgaggccaa ggcgcaggcc
     3241 gaggcgctgg gggccaaggt ggcgggatcg gtctcggcga aaaccgacct ggtcgtcgcc
     3301 ggccccggcg cgggctcgaa gctcaagaag gccgcggagc tcggcatcga tgtgatcgat
     3361 gaagccgcct ggaacgatct ggtcgcagct gccggcggct gagcgtccgg cagcccctgt
     3421 ggcggccgca attgaacggg atggtcggcg gttcgccaca tgctaacatc gcgccgtgat
     3481 cgcgggagaa aagtcatggc tagcgcagac gaaagaatcc ttgccgtgct gtttcacgcg
     3541 ttcacctccg gcccttcgaa gctggaaggc gtcgcacgga tcgtgcggga ggcctatcca
     3601 caggcggcgc tcgagataca ttgtcctgcg cttcccctga gcctcttctc gactgccgac
     3661 ccggcccaag ttgcttcgga ccaagcggac gagattgggc agctttgcgc ggacgcggag
     3721 cgcgccggtt cgcctttcac gcggatcgtc ttcgtggggc acagcattgg ctccctgctg
     3781 gcccgcaaag tgtacatcat cgcctgcggc gagaccgacc gcgccccttt cgaggaggcg
     3841 gtccgggaaa cggggccgcg gccgtgggcg cacctggtgg acaggatcat cctgctcgcc
     3901 ggcatgaata ggggctggcg gctttctcac catctcaatc cgctgcaggc tgcgggatgg
     3961 tggctgggga ccgtgttggg ccgcctgtgg gagcggatca ccggcggcaa ggtgctgatc
     4021 ctcggcatgc gtgtggggtc ggaattcatc acccagctac gggttcagtg gcttcgcatg
     4081 cgtcaagccg cccagcgcga cgagaaccga tcgggcaacg ccatgaccat ccagctgctc
     4141 ggctcgatcg acgacatggt ttcgccccgg gacaatgtcg acctcgtggc aggcagcgat
     4201 ttctattatc tcgacgtccc ttattccggc cacgcgagta tcatcgaagt cgacgaccgg
     4261 accgagccgc cgggcgccgg aagcaccgcc ggggcttggc ggagcgaagc gatccagcgg
     4321 gccctcgcca gctccaaggc cgatctcgct ccgctgcagg ttcttccggc ggatctcgat
     4381 gccgtgcccg cgcagcatcc cgatgtgacc gacgtggtgt tcgtcattca cggcatcagg
     4441 gatgcgggct attggaccta caaggtcgcg cggaaggttc gagaggcggc gggcgacaag
     4501 gcggccttca tcgcgaccga gacgtcgagc tacggctatt tcccgatgct gcctttccta
     4561 ttctcgttcc ggcgggccag gaacgtcgag tggctgatgg acgaatatgc cgctgccgtg
     4621 gcgctgtacc cccacgccgc gcgcttccac ttcatcgggc acagcaacgg cacctattgt
     4681 ctggtcgagg ccctgcgccg ctacgcctgc tgccacttct cccgggtggt gctggctgga
     4741 gcggtggtcc gcagccggct ggactggtcg gctttcctgc gcgacgagaa caggccggac
     4801 cagcggcggc ggcggcacgg cagcgtggag caggtgctga acttcgtggc gaccggcgat
     4861 ttggtggtgg ccgccttccc tcgcctgttc gagaccttcc tgccgatcca gcggctcggg
     4921 ggcgccggcc atatcggttt cctcgacgca ccgaacgcgg tgaagaatgt cgaatatgtc
     4981 aagggcggcc acggcgccgg tgtcgcggag ccctggtggc cgagcatcgc gcggttcatc
     5041 cacaccggtt cggtcgagcc gttcccgccg ggtctcaagc tggatcggca agcctggctg
     5101 gcccggctgc tcggggtgct gccgctgctc gggtggctgg ttgcggtggg gatcgccgtc
     5161 ctcctcggct ggggagttta tctggcggcg gaatgggtgt cggccctgct cgtcgcgaag
     5221 ggcgggcccg gtgcgaatgc gctcctccgc ttgctgtccc ccgccgccga ttgggtggtc
     5281 cccgtgacga tgacgctcta ctgcctcgcc gtcatcaagc tgttgcgctg ggtgtagcgc
     5341 ccccgcgttt caccgccagc ccgtcgccct gcgcttgcag cggcgcctct ttcctatccg
     5401 ttcgttctat gttccacatc ggcgatgttc cgaatcgctc gccgccgcct cgccgcctgc
     5461 ttgccccgcg cgcgcacata cgtacgagcg cgtgcgcgcg cgagagagac tgactttcct
     5521 gaccttcgtc cggatccgga cacattgggc tcagcgccag ccggtcgccc ggcgcttgcg
     5581 catgacgagc gtcggccact cgccattgtc cactcgaaag gcgagcatca ggccgcggcg
     5641 gcgataggag gcggcgacgt cgtcggcctg gcggtcgagc aggccggcca ggatgaggcg
     5701 gccgccgggc gcgatcgcgg ccgccaccgc cggggcaagc tcgatcagcg ggccggcgag
     5761 aatgttggcg atgagaaggt cgtagggcgc gcgcgcccgc aggcgcatgt gatcgagccc
     5821 ggcggcgacg gcgagctcga cctggccgcg ggcgcggccg agcgggaggc ggttgatcgc
     5881 cgcattctct tcggcgacct cgatcgacac cgggtcgatg tccgaagccg ccaccttcgc
     5941 cgccggccac agcttcagcg cggcaaaggc gagcagcccg gtgccggtgc cgagatcgag
     6001 gatgttggag acggcggcgc cggtgccctt gaggcggtcg agcgcgacga ggcagccggt
     6061 cgtcgtctca tgctgcccgg tgccgaaagc gcggccggcg tcgatctcga gcgggatcgc
     6121 atcggcgggg atttgatcgc ggtgcgcggg ggtgtggacg aagaagcggc cggcctggat
     6181 cggctccagc ccctgctggc tgagcgtcac ccaatcggcg tcggcgagct gctcgacccg
     6241 cggctcggcc gcggccgccg ccggagcaag cgccttcagc atcgccaccg tggcggcgct
     6301 cggcgcctcc tcgaaataag cgtcgagccg ccaatcgtcg gggcgtgcgg gatcggcctc
     6361 gctggtcatc agcaccggcg gcggatcgag caaagcgagc ggcccgatct cgccctgcag
     6421 ggcctctgcc tcggccttgg tgcagggcag ggtgaccttc cagctcgtca ccttcctgtg
     6481 ctcctgcgaa cgcaggagcc tagcccggct gccccctggg ctcctgcttc cgcaggagca
     6541 cgatcagcgg acatagctcg ccccgttgac gtcgatcacc gccccggtcg ccgaggcggg
     6601 cgcgtcgacc gccagccagc gcaccgtctc ggccacttcc tcggcggacg cgacccggcc
     6661 gagcgggatg tcggccagca atttgtcgcc gccgcggctt tcgagataat cctcggccat
     6721 gccggtcatg gtgaagccgg ggcagaccgc gaaggcgagg atattctcgc cggcatagcc
     6781 gcgcgcgatc gacttggtca tgccgatcat gccggccttg gaggcggcat aatgccaatg
     6841 ctggggcgaa tcgccgcgat aggcggcgcg gctcgacacg ttgacgatcc ggcctccccc
     6901 atttccgcgg aagtgcagca ccgccagccg gcacaattcc gccgaagcgg taaggttgat
     6961 ccgcatcgtc cgctcccagg cggcggacca gtcggcggcg ggcgattcga tcggcgcggc
     7021 ttcgaagatg ccggcattgt tgaccagcac gtcgatgctg ccgccgagcc gctccagcgc
     7081 ctccccccac agccgctccg ccgccccggg ctcggagagg tcggccgcga ccaggccctc
     7141 gccgccgcgg gtcgagtggc cgactgcctt gtgctggtcg agcgccgcga ggatggactt
     7201 gccgatgccc cggctggagc cggtcacgag gatgttgagc tgggtcatgg gcggccgtta
     7261 cggtcgcccg gcgcgaaggg gaaggggttc cgagccaagc cgctcccgtt ccccggcgaa
     7321 agccggtcgt tcccgcgaag gcggtccgta cccccgcgaa tgcgggggtc cagttcacgc
     7381 ccgtgcccaa gactggacct aagcggcgaa gctctcgacg aaatggctgg tggcggcggt
     7441 gaacctcgcc atctgctcgt cgacttcgcc gaaattgcgc tcgacctcgt cgatctcggt
     7501 gaccaccgtc tcggtgtcgg cgcggatagc ggcgatggtc gaggacatcg aatcggcggc
     7561 gagcgcggtc tcgtcgaccg cggcggtgat catcgtcacc gtctgcgcct gcacttccat
     7621 tgcccggcgg atgcggtcgg ccgagctttg cacttcggcc accgtctcgc ggatcgaacc
     7681 gttggcctcg acggtctggc gggtggcctg ctggatggcg ctgatcttgg cggtgatgtc
     7741 gtcggtcgcg cgcgcggtct ggctggccag cgacttcact tcctgcgcaa ctaccgcgaa
     7801 gccgcggccg gcgtcgccgg cgcgcgccgc ctcgatggtg gcgttcaagg cgagcaggtt
     7861 ggtctggccg gcaatgtcgc ggatcaggcc gaggatcgat tcgatcgcct cgacatgggc
     7921 ggacagcgcc tggctgacca gcaccgcctt cgacgccttg tcgccggcgc gggtggcgat
     7981 gccggcggcg acctcgacct cgctgcgcgc atcctcgatc gcctggatga ggccggccgc
     8041 ggtctgcgcc gcttcgcgca tcgcgatcgc cgattgctcg gcggcggcgg cgacttcgga
     8101 ggttttgccg agcatgccgc gcgcggccgc cgaggcgtcg gcggcctggg cgcgcaggct
     8161 gctgctttcg gaggcggcgc ggccgacggc gtgcagcacc tcgtggttga attcggcgcc
     8221 gcgggcgctg cggctctcgc gttcgatgcg gctgcggatg ccgtcataat gagcggagaa
     8281 tatttccact tcgagcaagg tcgcctgggt gagcgcgcgc accagccggg cgagcccggc
     8341 gggatcgccg gcgaggcggc gcacgagtat gtcctggatg cgcgcggtgg cggcggaaat
     8401 gccggagatg atggcggtca gcggcccttc gccggcggcg acctcggcga gctcgccgac
     8461 ccggtcgacc caggcctggc cggtgaggcc gcgaaacttc tgctcgacat aggggagcag
     8521 ccgcgcggcc attgcggagg cggcgtcgga tgccgcgccg gtcagcgcgc cggcgccgat
     8581 cgagcgcacg taccggtcgg cgaaagcgag cgcgatctcg tcccgctcgg gctcgatcag
     8641 cgcccagatt tcgcgcccga gctcgcttag gctgccgtcc ggatcgaaca gccccgcccg
     8701 atcctcgatc cgcacccgcc cctgttccgc tgtcgccacc ttgaaccacc ttcgctgatt
     8761 tggtggtcag gtggtacgga gcgacccgtt aagatggcgt tagccatcgg cggcggggcc
     8821 tcacccgagc aggtctgtcc tagtagtgga ccgcccgccc ataagcatcc agcacactct
     8881 cgtgcatcat ctcgctcaag gtcgggtgcg ggaagatggt gtgcatcagg tcggcctcgg
     8941 tggtctcgag ctggcgggcc acggcatagc cctggataag ttcggtgacc tcggcaccga
     9001 ccatgtgggc gccgagcagc tcgccggtgg cggcgtcgaa cacggtcttt acgaagcctt
     9061 cggcttcgcc cagcgcgatc gccttgccgt tgccgatgaa ggggaatttg ccgaccttga
     9121 cctggcggcc ctcctccctg gccttggctt cggtgaggcc gacggaggcg acctgggggc
     9181 gggaataggt gcagccgggg atgttccagg tctcgaacgg gtgcgggttg ttgccggcga
     9241 tggcttcgac gcagacgacg ccctcgtggc tggccttgtg ggcgagccag ggcggaccgg
     9301 tgacgtcgcc gatcgcgtag acgccctcga cattggtgcg gccgtagccg tcggtgacga
     9361 tatggccgcg ctcggtcttc acgccgagcg cttcgagccc gatattttcg gtgttcggca
     9421 cgatgccgat cgcgacgatg cagtggctga actcttcggt ggcgccgcct ttgagcgtcg
     9481 cgcggacgcc ggttgcgccg gtctcgagct tctcgacccc cgaggagacg tggatcttca
     9541 tgccctgctt ggtgagcgcc ttgtgcacga aggcggagat gtcgccatcc tcgaccggca
     9601 ggatgcggtc gagcatttcg acgatggtca cctcggcgcc catgtccgaa tagaagctgg
     9661 cgaactcgac cccgatcgcg ccggagccga tcaccagcaa tttggtcggc atcaccttcg
     9721 gcaccatcgc gtggcgatag gtccagatcc gctcgccgtc ggtcttggcg aaggggaggt
     9781 cgcgggcgcg ggcgccggtg gcgacgatga tgtgtttggc ctggagcgtc ttgcctgccc
     9841 cggaccccga tccggggtcg ccgaccttca gaatgccctt gccggccagc gcgccttcgc
     9901 cttcgacgac cgttatcttg ttcttcttca tcaggccttt gacgccggca ttgagctgcc
     9961 cggcgacctt gcggctgcgg tcgacgattt tggcgaggtc gaacgacacc ttctcggcgt
    10021 tgaggccgta gctggcggcg tgggtcagat aatggttgat ctcggacgtg cgcagcagcg
    10081 ccttggtcgg gatgcagccc cagttgaggc agatgccgcc cagtttctcg cgctcgacga
    10141 tcgccacctt catgccgagc tgcgccgcgc ggatggcggc gacatagccg ccggggccgg
    10201 agccgaggac gatgaggtcg taagtgtcag ccataatggt ttcacctgat gatccgagtg
    10261 gaagcctcag ccgccaactg ctccttgacg gtaacggctc cgatcaccgc tgccggaaga
    10321 aggactagcg cgagcaggat tttggcgcct gggctcctgc cagatttgaa gaagaaccca
    10381 agcgcgacga gcagggccaa gtcataacca aagacgatcg cggaccgatc ggaagacacc
    10441 ggcggggacg tcaggaccag cccgttgaca atcactatcg ccgccaacgc cgcggcggcc
    10501 aaaagccaca tcagggcggc gctcatcagc tggatgcgcg cctcccatgc cagggtcacg
    10561 cgatcatccc cagcgggttt tcgacgaggc gcttgaaggc ctgcatcagg cgggcgccgt
    10621 cggcgccgtc gatggcgcgg tggtcgaagc tgccggtggc gctcatcatg gtcgcgatct
    10681 gcaagctgtc gtcgatcacg tgcggccgct tctcgccggc gccgatcgcc atgatcatgc
    10741 cctgcggcgg attgatcacg gcctggaagt tggcgatgcc gtacatgccc atgttggaca
    10801 ggctcgcggt gccgccctga tattcgtgcg gctggagctt gccctcgcgg gcgcgggcgg
    10861 cgaggtcctt catctcggtg gaaatggccg acaggctgcg cgaacccgca tcgacgatga
    10921 tcggggtgat gaggccggcg gggatcgaga cggcgacgga aatgtcggcg cgcttaaagg
    10981 tgacgagctc ggccccggta tattggacgt tgcattccgg cacttcgcgc aaagcggcag
    11041 cgagcgcctt gatcatgaaa tcgttgacgc tgagcttgac gccgcggctc tccaggctgg
    11101 cgttgagctc ggcgcgcagc ttcatcagcg cgtcgagccg gacgtcgacg gtgagatata
    11161 tgtgcgggac ctgctgcttc gattcggtga ggcgccgcgc gatcgtcttg cggatgttgg
    11221 aaagcttgac cgcgtcgtgc gggatatcgg gcacgccgtg agcaggcgcg ggggcggggg
    11281 cggccgccgg tgcgggcgcg gcggcctgag ccggagcggc ggccggcgca gcggcgacgg
    11341 gcgcggcctt gccgctggcg gcgtctatgt cggccttgac gatgcggccg ccggggcccg
    11401 agccggtgag ggtggctagg tccaggccct tctgctcggc aaggcggcgg gcgagcgggc
    11461 tcgccttgat gcggtcgccg gtgtcggcct tgggctgcgg ctgcggctgg gccagcttct
    11521 cgtcctcggc cgggctggcg ccatagcctt tcacttcggg ggtcggcgtt ggggtcggag
    11581 tcggggtggc gggctggccg gcgccggtct cgccgccgcc tttggcgacc ggctcgggcg
    11641 ccgcggtcgg cgcggccgcg gcggcgccgg catcctcgcc ctcctgagcg atcagcgcga
    11701 tcacggtgcc gaccttcact tcgtcggtgc cctcggcgac caggatcttc gcgataatgc
    11761 cctcgtcgac cgcttcgaac tccatcgtcg ccttgtcggt ctcgatctcg gcgagcagat
    11821 cgccgccgcc cagcctcaat cctcccctcc agcagttgcc ggaggggccg ggggtggccc
    11881 ttcttcttct tcttccaccg gcgaccgcgt caaagcctcc ccgctcgccc gccggctcgc
    11941 ggagcagcag ggcatcgacc tctccgccct caccggctcc ggccccgccg gccgcatcgt
    12001 gaaagcggat gtcgacgccg ccgcgggaaa acccaaggcg gc
//
LOCUS       DASVRK010000005         5526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_436733, whole genome shotgun sequence.
ACCESSION   DASVRK010000005 DASVRK010000000
VERSION     DASVRK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436733"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF589_00270"
     CDS             complement(<1..213)
                     /locus_tag="VF589_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256037.1"
                     /translation="MATFTGTDANEVIRPTLISATVAADPPGSLPSDADDRINAGGGN
                     DLVEAGGGSNIVDLGAGNDNFLWHGFI"
     gene            622..969
                     /locus_tag="VF589_00275"
     CDS             622..969
                     /locus_tag="VF589_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256038.1"
                     /translation="MSEDLVTLWRPVGPAELRLIEEAGMAAFPPRLPEQPIFYPVTTE
                     DYAVKIARDWNVPQSGSGFVTRFRVRRDFLDRYQVQEAGGRAHREYWIPAEELDAFNA
                     AIVGSIEVVREFR"
     gene            1063..1581
                     /locus_tag="VF589_00280"
     CDS             1063..1581
                     /locus_tag="VF589_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256039.1"
                     /translation="MDPASTFSPTRRFRIVMGLFIAGLVLSGVTAFPLLWEARLAADM
                     LGLGDATSPAGHKGLAGWILTIRFGLEDAYAAYPWIAYGTDWLAFAHLAIALFFIAPL
                     IDPPSSRGNLLAGMAACVGVVPLALICGQVRGIPFGWRLIDCSFGVIGFALLLYCLRL
                     LPRIAEERAQAG"
     gene            complement(1599..2867)
                     /locus_tag="VF589_00285"
     CDS             complement(1599..2867)
                     /locus_tag="VF589_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX8256040.1"
                     /translation="MPIGKATIPALAAAMTIAAPAAAQPAQPATVEALGAELAEARAQ
                     IAEQRRALDAQEARLQALEARMAGGAASAAAAAPPAAAAPRYAGQANGVEQVGQAPAE
                     IEMPEVAVLGEQGSVITNAGQLSAEAQVDYTRADRNRVLFRGIEVVESVLIGVFDINE
                     SRQDVLTASAALRYGLTDRLEIGIRVPYVYRSDTSIITPVQGDSDMSIDNSTNGSGIG
                     DVEFTARYQLTSGRRGSPFLIANLQVVAPTGSSPFEVPRDAQGRALDASTGAGFWAIA
                     PSLTAILPTDPAVLFGTLGYTHNFGDEVDTRIPPVIIERVKPGDSVSASAGLGIALNQ
                     RTSFNLGYAHSWSFGTETTTRLIDPGPNDPGSITQTSRDLQIGRFLFGVTYRLSDAAS
                     VNWAVEVGATEDAPDVRTVLRIPIVLSQGR"
     gene            complement(2890..3447)
                     /locus_tag="VF589_00290"
     CDS             complement(2890..3447)
                     /locus_tag="VF589_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256041.1"
                     /translation="MRSTVKLIATLAGLAAAFPAAAAGGAFGRPAVSESRLGGMRGGF
                     LLPGGLDVALTVQSVTQLNGRVLFKTEFAANDGPAELNVSNGSRDLGVRVGGGGVRTG
                     GGTVEAVADRGGTRITYSARGLDVTHLAGIANGSIIANQRNGVSIDTATNVDLTLRNA
                     TPLNLGSTLLRVDSIAVDAATRLAH"
     gene            complement(3450..4175)
                     /locus_tag="VF589_00295"
     CDS             complement(3450..4175)
                     /locus_tag="VF589_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX8256042.1"
                     /translation="MIGRPLKLGRRWLGLAAAGAALAACQGTLSEQAAIAGGFGPVGD
                     VRIPIASVEERRFATVIRQRYDFSCGSAALATLLRYHYGLGQDEEAAFRGMWAKGDRD
                     QIRRLGFSLLDMKRYLAALGLDADGFEVTLDEVAKAGVPGIALVNLGGYRHFVVVKGV
                     ANGEVLVGDPALGLKPMKEREFKAAWNGVYFAVNSRLEKGRANFNRDAQWAQFARAPA
                     GAGFTDPLSQQALMLTAPFYREF"
     gene            complement(4172..4576)
                     /locus_tag="VF589_00300"
     CDS             complement(4172..4576)
                     /locus_tag="VF589_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256043.1"
                     /translation="MNYRYLKLIAGAGLALAPVSAAFAAPERPDEVHPTAASETSSAE
                     RPFAAASAVSDAALDEATAREDLSLVAETEQASEVSGNSVTGRSSTGDIRIADQAFES
                     ASGMTIVNANSGNNVAINASVNVNIVVAPPQP"
     gene            complement(4643..>5526)
                     /locus_tag="VF589_00305"
     CDS             complement(4643..>5526)
                     /locus_tag="VF589_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256044.1"
                     /translation="DGDGAMSTATTTDNSMTTDNSTDTRTANSNNTRTANSNNDNSDN
                     STETRTANSNNDNRRTDNSTDERTANSNNTRTAHSNNDNRRTDNSTDERTANSNNDNR
                     RTDNSTDERTANSNNTRTANSNNTRTANSNNDNSDNSTNDNSDNSTNDSSDNSTHNSN
                     NDHSVANTGSATSEGAFGNRSTVASANLASHVSGVNVTFEGGGGTGGQGGTGGTGGAT
                     GETGGRGGVGGRGEAGEGGASSMDNSLRVGGSAFQNFAGMNATNMNTGIAASQNANVS
                     VAVSTRDVAGTLPSTPQ"
BASE COUNT          722 a         1748 c         2055 g         1001 t
ORIGIN      
        1 gatgaagccg tgccacagga aattgtcgtt gccggcgccg agatcgacga tgttgcttcc
       61 gccgccggcc tcgacgaggt cgttgccgcc gcccgcgttg atcctatcgt cggcatcgga
      121 cgggagcgat ccgggcggat cagcggcgac ggtcgcgctg atcaaggtgg gccggattac
      181 ttcgttggcg tcagtcccgg tgaaggtagc catggtcggt ccccctgggg gcggcagcaa
      241 agacagagtc gaacggcgcc gcggctggct tgaggattcg gactgtacag gtcgctcggg
      301 ctccagtcga cttagaatgc ggaaagttca tctaaaaaag aacgtcgtgg gaagcgccgg
      361 cttcttcgtt cgaccaggcg cggctccgga tgtccttcgc ctggagcatc caggccagcc
      421 agaaagaaga agtcggttca cgcggaggcg cggaggcgcg gagggggcgg tggctgccgc
      481 ggcgggcgtg acttttcgac cggggggttg aacaggcgct tcgcgccgga ggcgctattt
      541 tcctgctccg cgtctccgcg cctccgcgtg aacccagatc aatgggtggg caatgggcat
      601 cggccggcct agcgtcgcgc catgagcgag gatcttgtca cgctgtggcg gccggtcggc
      661 ccggccgagc tgcgcctgat cgaggaggcg gggatggcgg cctttccccc gcgccttccc
      721 gaacagccga tcttctaccc ggttacgacc gaagattatg ccgtgaagat cgcccgcgac
      781 tggaacgtgc cgcaaagcgg atcaggcttc gtcacccggt tccgggtgcg gcgagacttc
      841 cttgaccgct accaagtgca ggaggcgggc ggcagggccc accgggaata ttggatcccg
      901 gccgaggagc tcgacgcctt caacgccgcc atcgtcgggt cgatcgaggt ggtgcgcgag
      961 tttcgctgag ggtccgcagc gggtgggcag cggatattgg gaagcgcccc tgttgcgggg
     1021 caatggcggg cattgccggg gcctgctagg ggctggcgcg cgatggaccc ggcttcaacc
     1081 ttctcgccga cgcggcgctt ccggatcgtc atggggcttt tcatcgccgg cctggtgctg
     1141 agcggagtca ccgcctttcc cctgctgtgg gaggcgcggc ttgcggccga catgctcggg
     1201 ctcggcgatg cgacctcgcc ggcggggcat aaggggctgg ccgggtggat cctgaccatc
     1261 cgcttcgggt tggaggatgc ctatgccgcc tatccctgga tcgcctacgg caccgactgg
     1321 ctcgctttcg cgcatctggc gatcgccttg ttcttcatcg cgccgctgat cgacccgcct
     1381 tcgagccgcg gcaatctgct cgccgggatg gcggcctgcg tcggcgtcgt gccgctggcg
     1441 ctgatctgcg ggcaggtgcg cggcattccg ttcggctggc ggctgatcga ctgctcgttc
     1501 ggcgtgatcg gcttcgcctt gctgctctat tgcctgcgcc tgctgccgcg gatcgcggag
     1561 gagcgggcgc aggccggctg acggggcgcg ggcgggagtc agcgcccctg tgagagcacg
     1621 atcgggatgc gcagcacggt gcggacgtcg ggcgcgtctt cggtggcgcc gacttcgacc
     1681 gcccagttga cgctggcggc gtcgctcagg cgataggtaa cgccgaacag gaagcggccg
     1741 atctggaggt cgcgcgaggt ctgggtgatc gacccagggt cgtttgggcc cgggtcgatc
     1801 agccgggtgg tggtctcggt gccgaacgac cagctgtggg cgtagccgag gttgaacgag
     1861 gtccgctggt tgagcgcgat gccgaggccg gcgctggccg agaccgagtc gccgggcttc
     1921 acccgctcga tgatcaccgg cgggatgcgg gtatcgacct cgtcgccgaa attgtgggtg
     1981 tagccgagcg tcccgaacag caccgcgggg tcggtcggga ggatcgcggt gaggctcggc
     2041 gcaatcgccc agaagccggc gccggtcgag gcgtcgagcg cccgcccctg ggcgtcgcgc
     2101 ggcacttcga acgggctcga gccggtcggg gcgacgacct gcaagttggc gatcaggaag
     2161 ggcgagccgc gccggccgga ggtcagctgg tagcgcgccg tgaattcgac gtcgccgatg
     2221 ccgctgccgt tggtgctgtt gtcgatgctc atgtcgctgt cgccctggac gggcgtgatg
     2281 atcgacgtgt cggagcggta gacgtagggg acacggatgc cgatctcgag tcggtcggtg
     2341 aggccgtagc ggagcgcggc ggatgcggtc agcacgtcct gccggctttc gttgatgtcg
     2401 aacacgccga tcagcaccga ttccaccacc tcgatgccgc ggaagagaac gcggttgcgg
     2461 tcggcgcggg tataatcgac ctgggcttcg gcgctcaatt ggccggcatt ggtgatgacg
     2521 ctgccctgct cgccgagcac cgccacttcg ggcatttcga tctcggcggg ggcctggccg
     2581 acctgctcga cgccgttcgc ctggccggcg tagcgcggcg cggccgcggc cgggggagcg
     2641 gcggcggcgg ccgacgcggc gccgcccgcc atccgcgcct cgagcgcctg cagccgcgcc
     2701 tcctgggcgt cgagcgcgcg gcgctgctcg gcgatctgcg cccgcgcctc ggcaagttcg
     2761 gcgccgagcg cctcgaccgt cgccggctgc gccggctgcg ccgccgccgg cgcggcgatg
     2821 gtcatggcgg ccgccagggc cgggatggtg gctttgccga tcggcattgg aataccccct
     2881 ttggttcggt cagtgcgcga ggcgcgtcgc cgcatcgaca gcgatcgaat cgacccgcag
     2941 cagggtcgag ccgaggttga gcggcgtcgc gttgcggagc gtgagatcga cgttggtggc
     3001 ggtgtcgatg ctgacgccgt tgcgctggtt ggcgatgatc gagccgttgg caatgccggc
     3061 gagatgggtg acgtcgaggc cgcgcgccga ataagtgatg cgggtgccgc cgcggtcggc
     3121 gaccgcctcg acggtgccgc cgccggtgcg gacgccgccg ccgccgaccc tcacgcccag
     3181 gtcgcgcgag ccgttggaga cgttgagctc ggcgggaccg tcgttggcgg cgaactcggt
     3241 cttgaacagg acccgtccgt tcaattgggt gacgctctgc accgtcaggg ccacgtcgag
     3301 gccgccgggc aggaggaagc cgccgcgcat gccgccgagc cggctttcgg acacggccgg
     3361 gcgaccgaag gcgccgccgg ccgccgcggc ggggaaggcg gcggcgaggc ccgccagcgt
     3421 cgcgatcagc ttcaccgtgg aacgcatgat cagaattccc tgtaaaaagg cgcggtcagc
     3481 atcagcgcct gctggctgag cggatcggtg aagccggctc cggccggcgc ccgggcgaac
     3541 tgcgcccatt gcgcgtcgcg gttgaaattg gcgcggccct tttcgagcct ggaattgacg
     3601 gcgaaataga cgccgttcca ggccgccttg aactcgcgct ccttcatcgg cttgaggccg
     3661 agcgcgggat cgccgaccag cacttcgccg ttcgcgacgc ccttcaccac gacgaaatgg
     3721 cgatagccgc cgaggttgac gagcgcgatt cccggcacgc cggccttggc gacctcgtcc
     3781 agcgtcactt cgaacccgtc ggcgtcgagg ccgagggcgg cgagatagcg cttcatgtcg
     3841 agcagcgaga aaccgagccg gcggatctgg tcgcggtcgc ccttggccca catgccgcga
     3901 aaggcggcct cctcgtcctg gccgaggccg taatggtagc gcagcagcgt ggcgagcgcg
     3961 gccgagccgc agctgaaatc gtagcgctgc ctgatcacgg tcgcgaaccg gcgctcttcg
     4021 accgaagcga tcggaatccg gacgtcgccg acgggcccga agccgccggc gatcgcggct
     4081 tgctcggaaa gcgtgccctg gcaggcggcg agcgcggcgc cggcggcggc caggccgagc
     4141 cagcgtcgcc cgagtttgag cggccggccg atcatggctg cgggggcgcc accacgatgt
     4201 tgacgttcac cgaggcgttg atggcgacgt tgttgccgct gttggcgttg acgatcgtca
     4261 tgcccgaagc gctttcgaag gcctggtccg cgatgcggat atcgccggtg ctgctgcggc
     4321 cggtgacgct gttgccgctg acttcgctcg cctgctcggt ctcggcgacc agcgacaggt
     4381 cttcgcgggc ggtggcctcg tccagcgcgg catcgctgac cgcgctggcg gcggcgaagg
     4441 ggcgttcggc cgaggaagtc tcggacgcgg ccgtggggtg tacctcatcc gggcgctccg
     4501 gcgccgcgaa ggcggcgctg accggagcca gcgccaggcc ggctcccgca atgagcttca
     4561 gataacggta attcatacga ggctcccgac gcacgctgtt cagaaaaggc ggggcggccc
     4621 gctgggccgc cccggcctca gcttattgcg gggtcgaggg gagggtgccc gcgacgtcgc
     4681 gtgtggacac cgcgacgctg acattcgcat tctgcgaagc ggcgatgccg gtgttcatgt
     4741 tggtcgcgtt catgcccgcg aagttctgga aggcgctgcc gcccacccgc aggctgttgt
     4801 ccatcgagga ggctccgcct tcgccggctt cgccgcgccc gccgacgccg ccgcggccgc
     4861 cggtctcgcc ggtcgcaccg ccggtgccgc cggtgccgcc ctggccgccg gtgccgccgc
     4921 cgccttcgaa ggtgacgttc acgcccgaaa cgtggctggc gagattggcg ctggccacgg
     4981 tcgaacggtt gccgaacgca ccctcgctgg tcgcgctgcc ggtgttggcg acgctgtggt
     5041 cgttgttgct gttgtgggtc gaattgtcac tgctgtcatt cgtcgaattg tcgctgttgt
     5101 cgttcgtcga attgtcgcta ttgtcgttgt tgctgttcgc ggtgcgcgtg ttgttgctgt
     5161 tcgcggtacg cgtgttgttg ctgttggccg tgcgctcgtc ggtggagttg tcagtccgcc
     5221 gattgtcgtt gttgctgtta gccgtacgct cgtcggtgga attgtcagtc cgccggttgt
     5281 cgttgttgct atgcgccgtc cgcgtattgt tgctgttcgc cgtgcgctcg tcggtcgaat
     5341 tgtcggtccg ccgattatcg ttgttgctgt tggcggtgcg cgtctcggtc gagttgtcgc
     5401 tattgtcgtt gttgctgttg gcggtgcgcg tgttgttgct gttcgccgtc cgcgtatcgg
     5461 tcgaattgtc tgtggtcatc gaattatcgg tggtggtggc ggtgctcatg gcgccgtcgc
     5521 cgtcgg
//
LOCUS       DASVRK010000006         6391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_561401, whole genome shotgun sequence.
ACCESSION   DASVRK010000006 DASVRK010000000
VERSION     DASVRK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561401"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..823)
                     /gene="ccmC"
                     /locus_tag="VF589_00310"
     CDS             complement(110..823)
                     /gene="ccmC"
                     /locus_tag="VF589_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX8256045.1"
                     /translation="MHAYANPARFLKLARPLTPLLGWGGGVLIALGVGAGLFLAPPDY
                     LQGESVRILYVHVPAAWLGMAGWAGIAVASLMQLVWRHPLAAVAARAVAVPGALFTAL
                     CLVTGSLWGRPTWGTYWEWDGRMTSMLVLLFLYIAFIALADAERDKGGEGRLAAIFGL
                     VGAINLPIIHYSVLWWRTLHQGQSIGVRGSSIDSSMLWPLFASAIGFSLLFGAVVLMR
                     MRGALARQKVDARLRRMAA"
     gene            924..1841
                     /locus_tag="VF589_00315"
     CDS             924..1841
                     /locus_tag="VF589_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8256046.1"
                     /translation="MAAPLPSSRPEQNRLLGIALRIGATTCFGFMAAMIKLGYEAGAN
                     TVEMVFYRFAFGLPPLLVWIAASGNFGAWRTERPRAHLLRGAIGLTTMILAFSALAYL
                     PLAEATTIGFAAPLFAVMLSALLLREEVGRHRWSAVALGFAGVVIVMQPGGAALPLEG
                     LAIAILSALGVACVTITIRQIGRTEGTQTTVLWFTGLSMLAVGAAMPLFAQAHDAMTW
                     AILIALGLFGGLAQLLLTASLRYAPVSVIAPFDYAQLLWAVLLGWLLWETQPPATTWA
                     GAAVIIASGLYTLYREHKLGREKPRAAAF"
     gene            complement(2104..3153)
                     /locus_tag="VF589_00320"
     CDS             complement(2104..3153)
                     /locus_tag="VF589_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX8256047.1"
                     /translation="MQSPWDYPDFDDHEVVHFVTDRNTGLRAIIAVHSTHLGPGAGGT
                     RLWHYSNPGNAVADVLRLSRGMSYKNAMAGLALGGGKAVIVADAARTKSPEMFAAFGR
                     AVDGLGGKYVTAEDVGVTVPDLVEVSKHTRYVSGLPVAAGAVGGDPGPHTSYGVYLGV
                     KAAVRRGLGKDSLAGLHIAIQGAGSVAGGLARLAAKDGARISVADIDQGRAQKLAEEV
                     GGTVVAPEAIMTVEADVLSPNALGAILTPESIAALRVPVVAGGANNQLATPAEGDLIH
                     QRGILYAPDYVINAGGIINVASEYLKDADEAGVRAKIEQIPGRLEQIWEESASSGRNP
                     AAVADGMARRLIGRG"
     gene            complement(3188..3264)
                     /locus_tag="VF589_00325"
     tRNA            complement(3188..3264)
                     /locus_tag="VF589_00325"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3228..3230),aa:His,seq:gtg)
     gene            3366..4385
                     /locus_tag="VF589_00330"
     CDS             3366..4385
                     /locus_tag="VF589_00330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8256048.1"
                     /translation="MIPFSILDLAPIVEGGDAALAFRNALDLARHGERWGYRRFWMAE
                     HHSMPGVASAATAVALAHIGAGTSTIRIGAGGVMLPNHAPLVIAEQFGTLAALYPGRV
                     DLGLGRAPGTDMAAARALRRSLAAGPDQFPQDVVELMNYFAPAEPGQAVRAIPGEGLE
                     VPVWILGSSLYGAQLAAMLGLPYAFASHFAPQQMMEAIQVYRASFRPSEQLDRPYVML
                     GFNVFAADEEDEAHRLATSLMQAFVALRSGRPTPLQPPVPGYLDSLPLGQRAMLDQLF
                     ACSAIGAPAAVAEQIHAFVERTGADELMITSQIFDHRARLRSFEIAAAQIGAEPAPPS
                     LAFPA"
     gene            4401..4601
                     /locus_tag="VF589_00335"
     CDS             4401..4601
                     /locus_tag="VF589_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256049.1"
                     /translation="MAEDRAKISTGPNERAVNEPIAGTAPGLPDEARAPGEELPEPPS
                     DEEVQRIAEKLGAKDAASASRH"
     gene            4818..5099
                     /locus_tag="VF589_00340"
     CDS             4818..5099
                     /locus_tag="VF589_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256050.1"
                     /translation="MKGIGYLISTASVVLLAMVAWQSTLQNPDLKPLLIGGVLTSILG
                     MFLRYRAHERDRKERLELLERIGLGSERSGAGATAAARSPLNRPAGLPS"
     gene            complement(5142..5216)
                     /locus_tag="VF589_00345"
     tRNA            complement(5142..5216)
                     /locus_tag="VF589_00345"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5182..5184),aa:Val,seq:cac)
     gene            complement(5323..5598)
                     /locus_tag="VF589_00350"
     CDS             complement(5323..5598)
                     /locus_tag="VF589_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX8256051.1"
                     /translation="MNKQELIGQVADTTGLSRSEASRAVEGVFDVIGGALKKGDEVRL
                     VGFGTFSCSKRKASTGRNPRTGEPMQIKESTQPKFKAGKGLKDSVNV"
     gene            complement(5737..>6391)
                     /locus_tag="VF589_00355"
     CDS             complement(5737..>6391)
                     /locus_tag="VF589_00355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX8256052.1"
                     /translation="DADQTGVVTGLAWTEVGGELLTIEAVTVPGKGQIKTTGKLGEVM
                     QESVQAAFSFVKARSPSYGVKPSLIGRKDIHVHLPEGAVPKDGPSAGVGMVTAIISTL
                     TGIPVRRDIAMTGEVTLRGRVLPIGGLKEKLLAALRGGIKTVLIPAENEKDLAEIPAN
                     ITQGLEIVAVAHVDEVLARALATPVVPIEWTEADELAAVPPAELHGGDDPIGVSSRH"
BASE COUNT         1007 a         2384 c         1986 g         1014 t
ORIGIN      
        1 gcgtcaagat ccgcctcggc cttgcgcatc gacccatagc tggccgccac caggccgatc
       61 gtgccggcaa aggtcagcgc ataagcggcg agaatgaaag gccagtggtt cacgcggcca
      121 ttctccgcag gcgcgcgtcc accttctgac gggcgagcgc gccgcgcatc cgcatcagca
      181 ccaccgcgcc gaacagcaag gagaagccga tcgcggaggc gaacaagggc cacagcatgg
      241 aggagtcgat cgaggagccg cgcacgccga tgctctgccc ctggtgaagc gtgcgccacc
      301 ataggacgga gtaatggatg atcggcaggt tgatcgcgcc gaccaggccg aagatggcgg
      361 caagacgccc ctccccgcct ttgtcccgct ccgcatcggc aagcgcgata aaggcgatgt
      421 agaggaacag gaggacgagc atcgacgtca tccgcccgtc ccattcccaa taagtgcccc
      481 aggtcggccg gccccacagc gaaccggtga cgaggcaaag cgcggtgaac agggcgcccg
      541 gcaccgcgac cgcccgcgcc gccaccgccg ccagcgggtg ccgccagacc agctgcatca
      601 ggctcgccac cgcaatcccc gcccagcccg ccatgccgag ccaggcggcc ggcacatgga
      661 cgtagaggat ccgcacgctc tcgccctgga gataatcggg cggcgccagg aacagccccg
      721 cgcccacgcc cagcgcgatc agcacgccgc cgccccaccc caaaagcggc gtcagcgggc
      781 gtgcaagctt caggaagcgc gcgggattgg cgtaggcatg catgggataa ccggtattta
      841 gccgagcatc gcccggcatg gaagccaatc gtgcccgtcg atccgccttg cccgcgccgc
      901 cgcgccccgc caaagccacc gccatggccg cgcctctgcc ctcctcgcgc cccgagcaga
      961 accggctgct cggcatcgcg ctgcgcatcg gcgccaccac ctgcttcggc ttcatggcgg
     1021 cgatgatcaa gctcggctac gaagccggcg ccaacaccgt cgagatggtc ttctaccgct
     1081 tcgccttcgg cctgccgccc ttgctggtct ggatcgccgc ctcaggcaat ttcggcgcct
     1141 ggcgcaccga gcggccgcgc gcccacctgc tgcgcggcgc gatcggcctc accaccatga
     1201 tcctcgcctt cagcgcgctc gcttaccttc cgctcgccga ggcgaccacg atcggcttcg
     1261 ccgcgccttt gttcgcggtc atgctctcgg ccctgctgct gcgcgaggag gtcgggcggc
     1321 accgctggag cgcggtcgct ttgggctttg ccggcgtcgt catcgtcatg cagccgggcg
     1381 gcgccgcgct cccgctggag ggcctcgcca tcgccatcct ttcggcgctc ggcgtcgcct
     1441 gcgtcaccat caccatccgt cagatcggcc gcaccgaggg cacccagacc accgtgctct
     1501 ggttcaccgg cctgtcgatg ctggcggtcg gcgccgccat gccgctcttc gcccaggcgc
     1561 acgacgccat gacgtgggcg atcctaatcg cgctcggcct gttcggcggc ctcgcccagc
     1621 tcctgctcac cgcctcgctc cgctacgcgc cggtgtcggt gatcgcgccg ttcgactatg
     1681 cgcaattgct gtgggcggtc ctgctcggct ggctgctctg ggaaacccag ccgcccgcca
     1741 ccacctgggc cggcgccgcc gtcatcatcg ccagcggcct ctacaccttg taccgcgaac
     1801 acaagctcgg ccgcgaaaaa ccgcgggcgg ccgcgttctg aggtgggccg acgtggctcg
     1861 cctttcgaaa cccacccgct catcctgagg agggactgac cccggacttg atccggggga
     1921 aggcccgtct cgaaggaccg gaaccgtgcg tccttcgaga cgccgcttcg ccaagctcag
     1981 cggctcctca ggatgagcga attggcgtag ctttcgtgtt ccgggcgcgg tcccgttccc
     2041 cggcgaaagc cggggcccag tcttggccgg tgccgctgga cccccgcttt cgcgggggaa
     2101 caatcatccc cgcccgatca atcgccgcgc catcccgtcg gccaccgccg ccggattacg
     2161 cccgctcgac gcgctttcct cccagatctg ctcgagccgg ccggggatct gctcgatctt
     2221 cgcccgcacc ccggcttcgt cggcatcctt cagatattcc gacgccacgt tgatgatgcc
     2281 gcccgcattg atcacataat cgggcgcgta gaggatgccg cgctggtgga tgaggtcgcc
     2341 ctcagccggc gtggcgagct ggttgttggc gccgccggcg accaccggca cgcgcaaagc
     2401 cgcgatcgat tccggcgtca ggatcgcgcc cagcgcattc gggctcagca cgtcggcctc
     2461 gaccgtcatg atcgcctcgg gagccacgac cgtgccgccc acttcctcgg caagcttctg
     2521 cgcccgcccc tggtcgatgt cggcgacgct gatccgcgcc ccgtccttgg cggccaaccg
     2581 cgccagcccg cccgcgacgc tgcccgcgcc ctggatggcg atatggagcc cggcgaggct
     2641 gtccttgccg agcccccggc gcaccgccgc tttgacgccg aggtagacgc cgtagctggt
     2701 atgcgggccg gggtcgccgc cgaccgcgcc cgccgccacc ggcaggcccg agacgtagcg
     2761 cgtatgcttg gacacttcga ccagatcggg cacggtcacc ccgacatcct cggccgtcac
     2821 atatttgccg ccgagcccat caaccgcgcg gccgaacgcc gcgaacatct cgggcgactt
     2881 ggtgcgtgcc gcgtcggcca cgatcaccgc cttgccgccg ccgagcgcga gccccgccat
     2941 cgcattcttg tagctcatcc cgcgcgacag cctcagcacg tcggcgaccg cattgcccgg
     3001 attggaataa tgccacagcc gcgtcccccc ggcgccgggg ccgagatggg tcgaatggac
     3061 cgcgatgata gctcggaggc cggtgttccg gtcggtgacg aagtgcacga cctcatggtc
     3121 gtcaaaatcg ggataatccc aaggcgactg cattggctcg gattcctggt ctggatgtga
     3181 gtgagggtgg ggcgaccgac gggacttgaa cccgcgaccc tcggtaccac aaaccgatgc
     3241 tctaaccagc tgagctacga tcgccacatg cgcatcaaag gtgcgccgcg ctcataaggg
     3301 cggcccgccc ccagcgtcaa ggaaactcgg ctggcgcgca gcccgcggca ccccatatgg
     3361 cagccatgat cccattctcc atcctcgacc tcgccccgat cgtggaggga ggcgacgccg
     3421 cgctcgcctt ccgcaacgcg ctcgaccttg cccgccatgg cgaacgctgg ggctaccggc
     3481 gcttctggat ggcggagcat cattcgatgc cgggcgtcgc cagcgccgcc accgcggtcg
     3541 ccttggccca tatcggcgcc ggcacctcga ccatccggat cggcgccggc ggcgtcatgc
     3601 tccccaacca cgcgcctctg gtgatcgccg agcagttcgg gaccctggcc gcgctttatc
     3661 ccggccgcgt cgacctcggc ctcggccgcg ctccgggcac cgacatggcg gccgcgcgcg
     3721 ccctgcgccg cagcctcgcc gccggccccg accagttccc gcaggatgtc gtcgagctca
     3781 tgaattattt cgcccccgcc gaacccggcc aggcggtgcg cgcgataccc ggcgaagggc
     3841 tcgaagtccc ggtctggatc ctcggctcga gcctctacgg cgcccagctc gccgccatgc
     3901 tcggcctccc ttacgccttc gcctcgcact tcgcgccgca gcagatgatg gaggcgatcc
     3961 aggtctaccg cgcgagcttc cgcccgtccg agcagctcga ccggccctac gtcatgctcg
     4021 gcttcaacgt cttcgcggcc gacgaggagg atgaggcgca ccgcctcgcc acctcgctga
     4081 tgcaggcctt tgtcgccttg cgcagcggcc gcccgacccc gctccagccg cccgtgcccg
     4141 gctatctgga ctcgctcccg ctcgggcagc gggcgatgct cgaccagctg ttcgcctgct
     4201 cggcgatcgg cgcccccgcc gccgtggccg agcaaatcca tgccttcgtg gagcggaccg
     4261 gcgccgacga actaatgatc acctcgcaaa tcttcgacca ccgcgcccgc ctgcgctcgt
     4321 tcgagatcgc cgccgcccag atcggcgcgg aaccggcgcc gccctcgctc gcttttccgg
     4381 catgaaggaa aggatcaatc atggctgagg acagggcgaa gataagcacc gggcccaacg
     4441 agcgcgccgt caacgaaccg atcgccggca ccgcccccgg cctccccgac gaagcccgcg
     4501 ccccgggtga ggagctcccc gagccgccga gcgacgagga agtgcagcgc atcgccgaaa
     4561 agctcggcgc caaggacgcg gcgagcgcct cgcgtcatta gatctggtcg tccaacgggg
     4621 aagcccgtca ttccagcgca agctggaatc tcccttcttc ttcccccggc tcccaaagaa
     4681 aagtgagatg ccagctttcg ctggcatgac gatccacccg ccagacaacc ccccgcgctc
     4741 gttgcgcggc gggaaccatt gccgctttgt tacgctttta tgacagccgt tccgcggcta
     4801 tcagtaagga gcgtaagatg aaaggcatcg gctatctcat ctctaccgcc agcgtggtcc
     4861 tgctcgcgat ggtcgcctgg cagagcaccc tgcagaaccc cgatctcaag ccgctgctga
     4921 tcggcggcgt cctcacctcg atccttggca tgttcctgcg ctaccgcgcc catgagcgcg
     4981 accgcaagga acggctggaa ctgctggaga ggatcgggct cgggagcgag cggagcgggg
     5041 ctggtgccac ggccgcggcg cgctcgccgc tcaatcgccc ggccggcctg ccatcctgag
     5101 gtcagcctaa gcttcgtaaa tgaaggaccg gtgagaaagc gtggtgggcg cggtagggat
     5161 tgaacctacg accccacccg tgtgaagggt gtgctctacc actgagctac gcgcccacgc
     5221 ttgaatcgtg cggcctctag ggttggacgg gcgccatgtc cagccgcccg gcgccgcctc
     5281 caacccgccg atcaaacatt ggccaagcca gacgttggcc aatcagacgt tgaccgaatc
     5341 cttgaggccc ttgccggcct tgaacttggg ctgggtcgat tccttgatct gcatcggctc
     5401 gccggtgcgc gggttgcgtc ccgtggaggc cttgcgcttc gagcaggaga aggtgccaaa
     5461 gccgaccagg cgcacttcgt cgcccttctt gagcgcaccg ccgatgacgt cgaacacccc
     5521 ttcgacggcc ctgcttgcct cgctccggct gagcccggtc gtgtcggcga cctggccgat
     5581 tagctcctgc ttattcatcc tgaccatacc cctgttacaa tggaatcgcg gcaacgccga
     5641 accgccacct tcgaacatga acgttacagg agtcaaaagg aaaagcgcga atttccgggg
     5701 aaaatcgcgc cattccaggg tcggttcggg ggataatcag tggcggcttg aaacccctat
     5761 cggatcgtcg ccgccatgca gctcggccgg cggcaccgcc gccagctcgt ccgcctcggt
     5821 ccattcgatc ggcaccaccg gcgtcgccag cgcccgcgcc agcacttcgt ccacatgggc
     5881 gaccgcgacg atctccagcc cctgggtgat gttggccgga atctccgcca ggtccttctc
     5941 attctcggcc gggatcagca ccgtcttgat gccgccgcgc agcgccgcca gcaacttctc
     6001 cttgagcccg ccgatcggca gcacccgccc gcgcaaggtg acctcgccgg tcatggcgat
     6061 gtcgcggcgg accgggatgc cggtaagcgt cgagatgatc gccgtcacca tgcccacgcc
     6121 ggccgacggc ccgtccttcg gcaccgcgcc ttcgggaagg tggacgtgga tgtccttgcg
     6181 gccgatcagg ctcggcttga caccgtagct tggcgagcgc gccttgacga aggagaaggc
     6241 cgcctggacc gattcctgca tcacttcgcc gagcttgccg gtggtcttga tctggccctt
     6301 gccgggcacc gtcaccgctt cgatggtcaa gagttcgccg ccgacctcgg tccaggcgag
     6361 gcccgtgacg acgccggtct ggtccgcgtc c
//
LOCUS       DASVRK010000007         6517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_499241, whole genome shotgun sequence.
ACCESSION   DASVRK010000007 DASVRK010000000
VERSION     DASVRK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499241"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF589_00360"
     CDS             complement(<1..486)
                     /locus_tag="VF589_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HEX8256053.1"
                     /translation="MTDPIPPSRVFYPASEEAETARHATSSPQTEDPAYRLAFQDLEF
                     LLREDLRPVRFQLELMKPSLLLDEAKIASTFVFYGSARIPEPAKADALILAASTDSQR
                     RIAERLKAKSVYYDEARKLARMASRCGPDEHGKRQFVVCSGGGPSIMEAANRGAADEG
                     VE"
     gene            complement(498..1145)
                     /locus_tag="VF589_00365"
     CDS             complement(498..1145)
                     /locus_tag="VF589_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX8256054.1"
                     /translation="MGRWGIIAGIALLAACSSSDDDKPRRAAAEPPRSEDAAKLTRQC
                     HAELRREDVEFKVLPDRRFGAGCSALGSVQLLEIGTPTANLGAMTCPLARNYARWTRE
                     AVQPAARAWLGSKVARIESFGTYACRPVNGMAGGKLSEHGRANAVDISAFVLANGQRI
                     TVLEGWNHEDDDVRRFLRAIHQAGCRRFGVTLGPEANKLHHNHFHFDMGRGPYCR"
     gene            1350..1928
                     /locus_tag="VF589_00370"
     CDS             1350..1928
                     /locus_tag="VF589_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX8256055.1"
                     /translation="MLWVLLAIPGLLMIHQYLTSDILPGDLLHPSGEWSARLMIFALM
                     LTPLSMLFRGQVWIQWLIRRRRAFGVAAFGYALLHLVLYVMDMETVRNILAEIGATGI
                     WTGWAALLLMLPVGLTSNEASMRILKKGWKRLQRLAYPAALLTLAHWLFIHDGKLAAL
                     LNFAPLAALEIYRLVRFFARARSSAGQRAAAT"
     gene            1955..2257
                     /locus_tag="VF589_00375"
     CDS             1955..2257
                     /locus_tag="VF589_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX8256056.1"
                     /translation="MTYLPRLILAASALALAAPALATGKMTCDAGPQSGWKSQDALKA
                     ALVKEGWQVRKSKVDGGCYEVYGTTPEGDRVEAYFHPVTFEKLLVSRRGEVLFKKK"
     gene            2447..3601
                     /locus_tag="VF589_00380"
     CDS             2447..3601
                     /locus_tag="VF589_00380"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX8256057.1"
                     /translation="MTNERPDVHPARPHFSSGPCAKPPGWGPEHLALGSLGRSHRSKL
                     GKARLGHAIELTRRLLRVPKTHRIGIVPGSDTGAMEMAMWSMLGARPVTMLAWESFGE
                     GWVTDVAKQLRLDANIVRAAYGELPDLAAVDPGHDVVFTWNGTTSGVRVPNGDWISET
                     REGLTLCDATSACFAMELPWDKLDVVTYSWQKVLGGEGGHGVLILSPRAVERLESYTP
                     PWPLPKIFRLTKAGKLNEGIFQGETINTPSMLAVEDYIFALEWGLGLGGLSALIARCE
                     ANRAALDAWVDRTPWIEHLAGDPATRSTTSVCLRFAPQAVRGLDEAGQAALVKSMATL
                     LEAEGAAFDIASYRDAPPGLRIWCGATVEADDVEALGPWLDWAFEEARTA"
     gene            3684..5261
                     /gene="serA"
                     /locus_tag="VF589_00385"
     CDS             3684..5261
                     /gene="serA"
                     /locus_tag="VF589_00385"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX8256058.1"
                     /translation="MPKVLISDPMDPKAAEILRARGIEVDEKPGLSRDELKAVIGGYD
                     GLAIRSATKVTSDLLEAADRLKVVGRAGIGVDNVDIPAASARGVVVMNTPFGNSITTA
                     EHAIALMFALARELPAADASTQSGKWEKNRFMGVELTSKTLGLIGAGNIGSIVAERAL
                     GLRMRVIAYDPFLTPERALDLGVEKVELADLLARADFITLHTPLTDQTRNILSRENLA
                     RTKPGVRIVNCARGGLIDEAALKQGLESGHIAGAALDVFTEEPAKASPLFGTPGFVAT
                     PHLGASTTEAQVNVAIQVAEQMADYLISGGVTNALNMPSLSAEEAPKLRPYMELAEKL
                     GSLVGQLEGKRLTGVSIEVEGAAAQLNQKPITGAVLAGLMHVYSDTVNMVNAPYLAKE
                     RGLDVREIRHDREGDYHTLVRVAVATPDGETSVAGTLFGNAAPRLVELFGIKVEAELG
                     GEMIYIVNDDQPGFIGRLGTALGEAGVNIGTFNLGRRAAGGEAVALVSVDSHIEQEVV
                     ARLERIPGVRRVKPLRF"
     gene            5326..>6517
                     /locus_tag="VF589_00390"
     CDS             5326..>6517
                     /locus_tag="VF589_00390"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX8256059.1"
                     /translation="MANVTVIGGQWGDEGKGKIVDWLASRADVVARFQGGHNAGHTLV
                     VGDQTYKLSLLPSGIVRGTLSVIGNGVVLDPWALRAEVEKLSGQGVAITPDNLQIADN
                     CPLILPFHRDLDALREDASGQGKIGTTRRGIGPAYEDKVGRRAIRVCDLAHLGDLGPQ
                     LDRLCAHHDALRAGFGEAPVDRERLTRDLGEVADFVLQFARPVWLTLNQAKARGQRIL
                     FEGAQGILLDVDHGTYPFVTSSNTIAGTAAGGTGVGPSAAGFVLGIVKAYTTRVGSGP
                     FPTELEDAIGQRLGERGREFGTVTGRKRRCGWFDAVLVRQSMAVSGVTGIALTKLDVL
                     DGLEEIRICTGYRLRGQTLDHLPAHAADQAAVEPVYETMEGWRETTAGARSWAELPAQ
                     AIKYI"
BASE COUNT         1019 a         2295 c         2159 g         1044 t
ORIGIN      
        1 ctcaacgcct tcgtccgcag cccccctatt ggctgcttcc atgatggaag gaccgccgcc
       61 ggagcagacg acgaactgcc ttttgccatg ttcgtcgggc ccgcagcggc tggccatgcg
      121 ggcgagcttg cgtgcctcgt cataataaac ggatttggcc ttgagccgct ccgcgatccg
      181 ccgctggctg tcggtcgatg cggcgaggat caaagcgtcg gccttggcgg gctcggggat
      241 gcgggccgat ccgtagaaaa cgaaggtcga ggcgatcttc gcctcatcga gcagcaaaga
      301 cggcttcatc agctcgagct ggaaacgcac cggccgcaga tcctcgcgga gcaggaattc
      361 gaggtcctgg aaggcgagcc ggtaggccgg gtcctcggtc tgcgggctcg acgtcgcgtg
      421 gcgcgcggtc tcggcttctt cgctggcggg atagaagacg cggctggggg gaatcgggtc
      481 ggtcatgcgc agcgccttta gcggcaatag ggcccccgtc ccatgtcgaa atggaagtga
      541 ttgtggtgca gcttgttggc ctcgggcccg agcgtcacgc cgaagcggcg gcagccggcc
      601 tggtggatgg cgcgaaggaa gcggcggacg tcgtcatcct catggttcca gccttcaagc
      661 accgtgatgc gctggccgtt ggcgagcacg aaggcggaga tgtcgaccgc attggcgcgg
      721 ccatgctcgc tcaatttgcc cccggccatg ccgttgaccg ggcggcaggc ataggtgccg
      781 aagctctcga tcctggcgac cttgctgccg agccaggcgc gggcggcggg ctgcacggcc
      841 tcgcgcgtcc agcgggcgta attgcgggcg agcggacagg tcatcgcgcc gagattggcg
      901 gtcggggtgc cgatctcgag cagctgcacc gatcccagcg ccgagcagcc ggcgccgaaa
      961 cggcggtccg gcagcacctt gaactcgaca tcctcgcgcc tgagctcggc gtggcattgg
     1021 cgggtaagct tcgccgcatc ctcgctgcgc gggggctcgg ccgcggcccg ccgcggcttg
     1081 tcgtcgtcgg acgacgaaca ggccgcgagc agcgcgatgc cggcgatgat cccccatctc
     1141 cccatcgcac gatgctgcgg gcgcgcgcgt cacggcgtca agccaagcgg ctgtaaagac
     1201 gagtcgaaac gtgcccgggg cgagtccggc cgttgagcgc ggcccgtcta ttgagaacct
     1261 ttcgcaataa gcttgcgcca gcctgtcgcg cccgctaccg gcagggcatg ggaagcggcg
     1321 cattaagatc aatcctggcg agcaggcttt tgctgtgggt cctgctcgcc attcccggcc
     1381 tgctgatgat ccatcaatat ctgaccagcg acatcctgcc cggcgatctc ctccacccga
     1441 gcggggaatg gtccgcccgg ctgatgatct tcgcgctgat gctgacgccg ctgtcgatgc
     1501 tgttccgggg ccaagtctgg atccagtggc tgatccgccg ccgccgcgcg ttcggcgtcg
     1561 ccgccttcgg ctacgctttg ctccacctcg tcctttacgt gatggacatg gagacggttc
     1621 gcaacatcct cgccgagatc ggcgcgaccg gcatctggac cggctgggcg gcgctgctgc
     1681 tgatgcttcc agtcggcctc accagcaacg aagcgtcgat gcgtatcttg aagaagggtt
     1741 ggaagcggct gcagcggctg gcctatccgg cggctttgct gacgcttgcc cactggctgt
     1801 tcatccatga tggcaagctt gccgccttgc tgaatttcgc cccgctcgcg gcgctcgaaa
     1861 tctatcgttt ggtccgtttt ttcgcccgcg cccgttcatc ggcgggccag cgtgccgccg
     1921 ccacatgacc cgcaacccac cggagaatcc gaccatgacc tatttgcccc gcctgatcct
     1981 cgccgcttcc gcgctcgcgc tcgccgctcc cgcgctcgcc accggcaaga tgacttgcga
     2041 cgccggcccg caatcgggct ggaagagcca ggacgcgctc aaggcggcgc tggtcaagga
     2101 aggctggcag gtccgcaagt cgaaggtcga cggcggctgc tacgaagtct acggcaccac
     2161 gcccgagggc gatcgggtcg aggcctattt ccacccggtg acgttcgaga aactgctcgt
     2221 ttcccgccgc ggcgaggtgc tgttcaaaaa gaagtgacgc tcccgacaga gcgtcacccc
     2281 gctgaaggcc ggggtgtcgt ggtggccggc cgtattttcc tggtccctcg attcccgcct
     2341 gcgcgggatg acggagatgt gttgacaccc ccgcgcccgg ccctatcccg gcggcgggcc
     2401 acgccgtccc aacgcggcgc gcgctctctg gaaggagaga ccgacagtga ccaatgaacg
     2461 gccggacgtg caccccgcgc gtcctcattt ctcttccggc ccgtgcgcca agccgcccgg
     2521 ctggggcccc gaacatctcg cgctgggttc gctcggccgc tcgcaccgct cgaagctcgg
     2581 caaggcccgg ctcggccacg cgatcgagct tacccggcgg ctgctgcggg tgccgaagac
     2641 gcatcggatc ggcatcgttc ccggctccga caccggcgcc atggaaatgg cgatgtggtc
     2701 gatgctcggc gcccgcccgg tgaccatgct cgcctgggaa agcttcggcg aaggttgggt
     2761 caccgacgtg gccaagcagc tcaggctcga cgccaatatc gtccgcgccg cttatggcga
     2821 gctgcccgat ctcgccgcgg tcgatcccgg ccacgacgtc gtcttcacct ggaacggcac
     2881 cacctcgggc gtgcgcgtgc cgaacggcga ctggataagc gagactcgcg aagggctcac
     2941 cctctgcgac gccacctcgg cctgcttcgc gatggagctg ccgtgggaca agctcgatgt
     3001 cgtcacctat agctggcaga aggtgctggg cggggagggc ggccacggcg tcctcatcct
     3061 ctcgccgcgc gcggtcgaac ggctcgaaag ctacacgccg ccctggccgc tgcccaagat
     3121 cttccgcctc accaaggcgg gcaagctcaa cgagggcatc ttccagggcg agacgatcaa
     3181 cacgccttcg atgctcgcgg tcgaggatta tatcttcgcg cttgaatggg ggctcggcct
     3241 cggcggcctg tcggcgctga tcgcccgctg cgaggccaac cgggccgcgc tcgacgcctg
     3301 ggtcgaccgg acgccctgga tcgagcatct cgccggcgat cccgccaccc gctcgaccac
     3361 cagcgtctgc ctcagattcg caccccaggc ggtgcgcggg ctcgacgagg cggggcaggc
     3421 ggctttggtc aagtcgatgg cgaccctgct tgaggccgaa ggcgccgcct tcgacatcgc
     3481 cagctaccgc gacgcgccgc cgggcctgcg catctggtgc ggcgccaccg tcgaggccga
     3541 cgacgtcgaa gcgctcggcc cctggctcga ctgggccttc gaagaggccc gcaccgcctg
     3601 acgagtcact cgaagagccg ttcaccggcg aaggccggtg cccagtctgg accccggcct
     3661 tcgccggggc acaaggaacc caaatgccca aagtcctgat ctccgacccg atggacccca
     3721 aagccgccga gatcctccgc gcgcgcggga tcgaggtcga cgagaagccc ggcctctcgc
     3781 gcgacgagct gaaggcggtg atcggcggct atgacggcct cgccatccgc tccgccacca
     3841 aggtcacaag cgacctgctc gaagcggcgg atcggctgaa ggtggtggga cgcgccggca
     3901 tcggcgtcga caatgtcgac atccccgccg cttcggccag gggcgtcgtc gtcatgaaca
     3961 cgccattcgg caattcgatc accaccgccg agcatgccat cgccctgatg ttcgcgctcg
     4021 cccgcgagtt gcccgccgcc gacgcctcca cccagagcgg caagtgggag aagaaccgct
     4081 tcatgggggt cgagctcacc tccaagacct tgggcctgat cggcgccggc aatatcggct
     4141 cgatcgtcgc cgagcgcgct ttgggcctca ggatgcgggt gatcgcctac gatccgttcc
     4201 tcacgcccga gcgcgcgctc gacctcggcg tcgagaaggt cgagctggcc gacctgctcg
     4261 cccgcgccga tttcatcacc ctccacaccc cgctcaccga ccagacccgc aacatcctct
     4321 cgcgcgagaa tctggcgcgg accaagcccg gcgtccggat cgtcaattgc gcgcgcggcg
     4381 gcctcatcga cgaggcggcg ctgaagcagg ggctggagag cggccatatc gccggcgccg
     4441 cgctcgacgt gttcaccgag gagccggcca aggccagccc cttgttcggc acgcccggct
     4501 tcgtcgccac cccccatctc ggcgcgtcca ccaccgaggc gcaggtcaat gtcgccatcc
     4561 aggtcgccga gcagatggcc gattatctca tcagcggcgg cgtcaccaac gcgctcaaca
     4621 tgccctcgct ctcggccgag gaggcgccga agctcaggcc ctatatggag ctggccgaga
     4681 agctcggctc attggtcggc cagctcgaag gcaagcgcct caccggggtg tcgatcgagg
     4741 tcgagggcgc ggccgcccag ctcaaccaga agccgattac cggcgccgtg ctcgccgggc
     4801 tgatgcacgt ctattccgac acggtgaaca tggtgaacgc gccctatctg gccaaagagc
     4861 gcggtctcga cgtgcgcgag atccgtcacg accgcgaggg cgattaccac acgctcgtcc
     4921 gcgtcgccgt cgccacgccg gacggcgaga cgtcggtcgc cggcaccttg ttcggcaacg
     4981 ccgcgccgcg cctggtcgaa ttgttcggca tcaaggtcga agccgagctc ggcggcgaga
     5041 tgatctacat cgtcaacgac gaccagcccg gcttcatcgg ccggctcggc accgcgctcg
     5101 gcgaggccgg ggtcaatatc ggcaccttca acctcggccg ccgcgccgcc gggggcgagg
     5161 cggtcgcttt ggtgtcggtg gacagccata tcgagcagga ggtcgtcgcc cggctcgagc
     5221 ggatccccgg cgtcaggcgg gtgaagccgc tccggttctg atcgctggac gttcggcggc
     5281 tcaaccccta gacgctcccg gtccatttcg gggagaggct cggccttggc gaacgttacc
     5341 gtgatcggcg gccagtgggg cgacgaaggc aagggcaaga tcgtcgactg gctcgccagc
     5401 cgcgccgatg tcgtcgcgcg cttccagggc ggccacaatg ccggccacac tctggtggtc
     5461 ggcgaccaga cctacaaatt gtcgctgctc ccgtcgggca tcgtccgcgg caccctgtcg
     5521 gtgatcggca acggcgtcgt gctcgatccc tgggcgctcc gcgccgaagt cgagaagctt
     5581 agcgggcagg gggtcgcgat cacgcccgac aacctccaga tcgccgataa ctgcccgctc
     5641 atcctgccct tccaccgcga tctcgacgcg ctgcgcgagg atgccagcgg ccagggcaag
     5701 atcggcacca cccgccgcgg cattggcccg gcttacgagg acaaggtcgg ccggcgcgcg
     5761 atccgggtct gcgacctcgc ccatctcggc gacctgggcc cgcagctcga ccggctgtgc
     5821 gcccatcacg atgcgctccg cgccggcttc ggcgaagcgc cggtcgaccg cgagcggctg
     5881 acccgcgatt tgggggaagt cgccgacttc gtactccagt tcgcccgccc cgtctggctc
     5941 accctcaacc aggccaaggc acgcggccag cgcatcctgt tcgaaggcgc gcagggcatt
     6001 ttgctcgacg tggatcacgg cacctatccg ttcgtgacct cgtccaacac catcgccggc
     6061 accgccgccg gcggcaccgg cgtcggcccg tcggcggccg gcttcgtgct cggcatcgtc
     6121 aaggcctata cgacccgcgt cggatccggc ccgttcccga ccgagctcga ggacgcgatc
     6181 ggccagcggc tcggcgagcg cggccgcgag ttcggcaccg tcacgggcag gaagcgccgc
     6241 tgcggctggt tcgacgcggt gctggtgcgc cagtcgatgg cggtctccgg agtcaccggc
     6301 atcgcgctca ccaaattgga cgtgctcgac gggctcgagg agatccgcat ctgcaccggc
     6361 tatcggcttc ggggccaaac cctcgatcat ctccccgccc acgccgccga ccaggccgcg
     6421 gtcgagcccg tctatgagac gatggagggc tggcgcgaga cgacggcggg cgcgcgcagc
     6481 tgggccgagc ttcccgccca ggcaatcaaa tatattc
//
LOCUS       DASVRK010000008         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_64006, whole genome shotgun sequence.
ACCESSION   DASVRK010000008 DASVRK010000000
VERSION     DASVRK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64006"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3204)
                     /locus_tag="VF589_00395"
     CDS             complement(<1..3204)
                     /locus_tag="VF589_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256060.1"
                     /translation="MADAGTSVETPPPGRHRARTAAKWAAIVLGILLLLVAGLFFGIN
                     SELGRRYVVNQINNLETASGLDIDVGRIEGSLYGELIIHDIALKDPKGTFFTAPRAEL
                     DWRPFAYFRNHIDIRGLTIPDAHLRRLPALRPGDPNAPLLPDIDIDIGRIEVGRLRVD
                     PAVTGQRHLLSLRGAAKISDGRAEIGASAQALTAPGIAGGDRLSLRLNAVPAADRFDL
                     DARLQAPGNGFVARMAGLDRPISAAISGRGSWANWQGQAQAALGGEAFAKLGVSARNG
                     TFSLVGPMRPGLLLADGPLQRLVAPLVQVNLVTTLAERRADLRLRLQSAAVSAAAEGL
                     VDLGNNRFEGLRVAARLLKPGAIAPALTGRDVQVAMVLNGAFATPVVAYDLRAAALGF
                     GETTVEGLTARGRARIDTDRITLPISARARRITGLNAAVGGLLTNVTLDGTLNMAGSR
                     ILSDNLRIRSDRVNATAIVVADLAKGEYRAGLQGRINNYLVRGIGLLDIESDLDVVAR
                     GAGFGIKGRVAVRTRRIDNASARDFLGGNALVTANVNMTETGIIGLDNIRLASPQLRI
                     TSGRGTYNPAGPINFTLSGVSTAYGPFSVVLTGTAAQPNVRLRAGNPGFGIGLRNVEA
                     DIRATAGGYRISARGQSQYGPFTADVTVRTGGGPLTIDVHRLVFAGFTFTGRVVQTAA
                     GPYAGTLRVSGQGVNGIVRLAAAGRHQRADISATANGAQIPGSPPILIQRGIIQATAV
                     LYPTAPSIVGDIQLAGVRTGETLVETARARINYQGGRGQAQVVATGRSGVPFQVAANV
                     ALTPTHIRAAAQGQINRLPFRLARPADVRKVGADWVLAPATLVFPQGNVRLAGRWGNG
                     LTIQSRVDNLDLAIANAFVPNLGVAGRITGSLDFAQPSGASFPRADARLLVQNFTRTG
                     VATLSEPVNLAFAGQLRPDGGSAAAVIRRRGAVIGRIQARLQPLGPGSGGWMTRLLAA
                     PLAGGVRYNGPAEVLWSLTGIADRQLSGPIGIAADFSGRVQDPQFTGIVRANNLTFVD
                     ETYGTRVINVGLQGRFTSSRLEVTQLSGRAGSGTVTGR"
     gene            complement(3204..>4033)
                     /locus_tag="VF589_00400"
     CDS             complement(3204..>4033)
                     /locus_tag="VF589_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8256061.1"
                     /translation="TFRRANAGQRDRTLLLIAEAGRRDYPAFRGYTAMLSGRIAREST
                     PIWQKRWTYAYGAELIATNETLFRREDNPEPTGTYFIGGLLGQLGYDRSDSLLNPTKG
                     FRLLGRINPEASLRDGVRPYIRNLIEGSAYYPIAEDFVIAGRARFGSIFGIERDALAP
                     SRRLYAGGGGSVRGFGYQELGPKDIEDNPIGGRALTEFALEGRYRFGDYGAVGFIDAG
                     QVYESEYPKLSNLRYGIGVGARLYTNFGPLRLDVATPLNRQPGESRIAVYVSIGQAF"
BASE COUNT          597 a         1236 c         1635 g          565 t
ORIGIN      
        1 gcgcccggtg acggtgccgc tgccggcgcg gccgctgagc tgggtgacct cgagccggga
       61 gctggtgaag cggccctgga ggccgacatt gatcacgcgg gtgccgtagg tctcgtcgac
      121 gaaggttaga ttgttggcgc ggacgatgcc ggtgaattgc ggatcttgga ctcgcccgga
      181 gaagtcggcg gcgatgccga tcgggccgga tagttgccgg tcggcgatgc cggtcagcga
      241 ccacagcacc tcggccggcc cgttatagcg gaccccgccg gcgagcgggg cggcgagcag
      301 gcgggtcatc cagccgccgg agccgggccc gagcggctgc aggcgggcct ggatgcggcc
      361 gatgacggcg ccgcggcggc ggatcacggc cgcggcagag ccgccgtcgg gccggagctg
      421 gccggcgaag gcgaggttga ccggctccga cagggtggcg acgccggtgc gggtgaagtt
      481 ctggaccagc aggcgggcgt cggcgcgcgg gaagctggcg ccgctcggct gggcgaaatc
      541 gaggctgccg gtgatgcggc cggcgacgcc gagattgggc acgaacgcgt tggcgatagc
      601 gagatcgaga ttgtccaccc gcgactggat ggtgaggccg ttgccccagc ggccggcgag
      661 acggacattg ccctgcggga acaccagggt ggcgggagcg agcacccagt cggcgccgac
      721 cttgcggacg tcggcggggc gggcgaggcg gaagggcagg cggttgatct ggccttgcgc
      781 ggcggcgcgg atgtgggtgg gcgtcagcgc gacgttggcg gcgacctgga agggcacgcc
      841 gctgcggccg gttgcgacca cctgcgcctg gccgcggccg ccctggtaat tgatgcgggc
      901 gcgcgcggtc tcgaccaaag tctcgccggt gcggacgccg gcgagctgga tgtcgccgac
      961 gatcgagggc gcggttggat agaggacggc ggtcgcctgg atgatgccgc gctggatcag
     1021 gatcggcggg ctgccgggga tctgggcgcc gttggcggtg gccgaaatgt cggcgcgctg
     1081 gtggcggccg gcggcggcga gccggacgat gccgttcacg ccttggccgc tgacccgcaa
     1141 ggtgccggca taggggccgg ccgcggtctg gacgacccgg ccggtgaagg tgaagccggc
     1201 gaagaccagg cgatggacgt cgatggtgag cgggccgccg ccggtgcgga ccgtcacgtc
     1261 ggcggtgaac gggccatatt gcgattggcc gcgcgcgctg atccggtagc cgccggcggt
     1321 cgcgcgaatg tccgcctcga cgttgcgcag gccgatgccg aagcccgggt tgccggcgcg
     1381 caggcgcacg ttgggctggg cggcagtgcc ggtgaggacg acgctgaacg gaccgtaggc
     1441 ggtcgagact ccggacaggg tgaaattgat cgggccggcg gggttgtagg tgccgcggcc
     1501 ggaggtgatg cgcagctgcg gcgaggcgag ccggatattg tcgaggccga tgatgccggt
     1561 ctcggtcatg ttgacattgg cggtgacgag cgcgttgccg cccaggaaat cgcgggccga
     1621 agcattgtcg atgcggcggg tgcggaccgc cacccggccc ttgatcccga agccggcgcc
     1681 gcgcgcgacc acgtcgaggt cgctctcgat atcgaggagg ccgatgccgc ggacgagata
     1741 attgttgatg cggccctgga ggccggcgcg atattcgccc ttggcgagat cggcgacgac
     1801 gatggcggtg gcgttcaccc ggtcggagcg gatgcgcagg ttgtcggaca ggatgcgcga
     1861 gcccgccatg ttgagcgtgc cgtcgagcgt gacgttggtg agcaggccgc ccaccgccgc
     1921 attgaggccg gtgattcggc gggcgcgggc ggagatcgga agcgtgatgc ggtcggtgtc
     1981 gatacgggcg cggccgcggg cggtgaggcc ttcgaccgtc gtctcgccga agccaagcgc
     2041 ggcggcgcgc agatcgtagg ccacgaccgg ggtggcgaag gcgccgttca ggaccatcgc
     2101 cacctgcacg tcgcggccgg tgagcgcggg cgcgatcgca ccgggcttga gcaggcgggc
     2161 ggcgacgcgc aggccctcga agcggttgtt gccgagatcg accaggcctt cggccgcggc
     2221 cgagacggcg gcgctctgca ggcggaggcg gaggtcggcg cggcgctcgg caagcgtggt
     2281 gacgagattg acctggacga gcggggcgac cagccgctgg agcgggccgt cggcaaggag
     2341 cagccccggg cgcatcggcc cgaccaatga gaaggtgccg ttgcgggcgc tgactccgag
     2401 cttggcgaaa gcctcgccgc cgagcgcggc ctgggcctgg ccctgccaat tggcccaact
     2461 gccgcggccg ctgatcgccg ccgagatcgg ccggtcgagc cccgccatgc gcgcgacgaa
     2521 gccgttgccg ggcgcctgca gccgcgcatc gaggtcgaag cggtcggccg ccgggaccgc
     2581 gttgaggcgc agcgaaagcc ggtcgccgcc ggcgatgccg ggcgcggtca gcgcctgggc
     2641 gctggcgccg atctcggcgc ggccgtcgga gattttggcg gcgccgcgca ggctgagcag
     2701 gtggcgctgg ccggtcaccg cgggatcgac gcggagccgg ccgacctcaa tccggccgat
     2761 gtcgatgtcg atgtccggca gcaagggcgc gttggggtcg ccgggcctga gcgcgggcag
     2821 ccgccgcaga tgcgcgtcgg ggatggtgag gccgcgtatg tcgatatggt tgcggaaata
     2881 ggcgaacggc cgccagtcga gctcggcgcg cggggcggtg aagaaggtgc ccttgggatc
     2941 cttgagcgcg atgtcgtgga tgatgagctc gccgtacagc gagccttcga tccgcccgac
     3001 gtcgatgtcg agcccggacg ccgtttcgag attgttgatc tggttgacga cgtagcggcg
     3061 gccaagctcg ctgttgatgc cgaagaacag gcccgcgacc agcagcagca ggatgccgag
     3121 gacgatcgcg gcccatttcg ccgcggtgcg cgcgcggtgg cggccgggcg gcggcgtctc
     3181 gacgctggtg ccggcgtcgg ccatcagaaa gcctgcccga tcgaaacgta gacggcgatg
     3241 cgcgattcgc caggctggcg gttgagcggg gttgcgacgt cgagccgaag cggcccgaaa
     3301 ttggtgtaga gccgggcgcc gacgccgatg ccgtagcgca ggttcgaaag cttgggatat
     3361 tcgctctcat agacctggcc ggcgtcgatg aagccgaccg cgccataatc gccgaatcgg
     3421 tagcgccctt cgagcgcgaa ttcggtaagg gcgcggccgc cgatcggatt gtcctcgata
     3481 tccttcgggc cgagttcctg atagccgaag ccgcgcaccg agccgccgcc gcccgcataa
     3541 agccgccgcg agggcgcgag cgcgtcgcgc tcgatgccga agatcgagcc gaagcgggcg
     3601 cggccggcga tcacgaaatc ctcggcaatc ggataataag cgctgccctc gatcaggttg
     3661 cggatatagg ggcggacgcc gtcgcggagc gacgcttcgg gattgatccg gccgagcagg
     3721 cggaagccct tggtcggatt gagcaggctg tccgaacggt cgtagccgag ctggccgagc
     3781 agcccgccga tgaaataggt gccggtcggc tcgggattgt cctcgcgccg gaacagggtc
     3841 tcgttggtgg cgatgagctc ggcgccataa gcgtaggtcc agcgcttctg ccagatcggc
     3901 gtcgattcgc gcgcgatccg gccggagagc atcgccgtat agccgcggaa ggcgggataa
     3961 tcgcggcggc cggcctcggc gatcagcagc aaagtccggt cgcgctggcc ggcattggcg
     4021 cggcggaagg tga
//
LOCUS       DASVRK010000009         6078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_126494, whole genome shotgun sequence.
ACCESSION   DASVRK010000009 DASVRK010000000
VERSION     DASVRK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126494"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1111
                     /locus_tag="VF589_00405"
     CDS             155..1111
                     /locus_tag="VF589_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256062.1"
                     /translation="MSDYGYSFYLYRESGYFDISIDYSIDGYHDPETFEYFGDTSWDY
                     SINGVGGGGGYGAESGNAFFSMGFGSGEQVEHHIFEFSAQNTFSAESISFRFDMLLAG
                     AATANLVIAGTDYSELIVSGSGADSLSGAGGRDHLDAGDGDDSIDGGDGNDLIDGGSG
                     ADLMAGGAGDDRYHVDRAADSIVELADGGGDHVLSRLSWTLSEHVEALTLSGPTARRG
                     IGNASDNLIYSNNLANTLAGLEGDDSLTGHGGDDLLHGGGGGDKLFGGLGLDTLKGGA
                     GDDTYLVDQASDILLEAAARASIRSRSSSRSRATRSAPISRT"
     gene            1108..1512
                     /locus_tag="VF589_00410"
     CDS             1108..1512
                     /locus_tag="VF589_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256063.1"
                     /translation="MTFTGNAEPFRGFGNVLDNVIAGARGDDRLFGMRGNDRLEGRVG
                     DDMLVGGGGTDSFVFATGQGRDRIADFDALGLETVAIAGGSHFDTFDEIMAAARSVGP
                     DGGSTLLKFGAGSTLLLTNVAMDDLTAANFGL"
     gene            1862..3259
                     /locus_tag="VF589_00415"
     CDS             1862..3259
                     /locus_tag="VF589_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256064.1"
                     /translation="MSSPYFIRGFTPDFDRQSGTLKIAVSYVIQPYSNPFTHFTSART
                     SWSYDIRGTNMAVNGSGEGSAAPTIRTAVEVQQTAGPQRFSFSFHAENTYSGAAVDLA
                     LNLLIDVYSAGDVIIGGTAGDDLIVAGVGDDVLRGGSGSDYLAGDSGDDILVGGGDRA
                     GDRLDGGAGADRMYGGFGNDRYRVNSRGDRVIERADADPNNKDIVEASVDHVLAANVE
                     ALWLLGAAGLNGGGNALDNQLGGNDGANRLSGFGGRDELHGKGGADILLGGAGNDVLY
                     GGEDLDEDRLAGGSGDDHYYGLAGDRIVEAAGGGTDFVWTHEAFFALPDHVENLDASG
                     RGTGSFRGLGNSGDNVIAGSFFADDRLAGRGGADTLSGSGGRDVLAGGGGTDTFGFAL
                     ESDVDKVTDFRAGADGERLALDLGSAFDTIEEVMAVAVFSGDRVVFDFGDGDRLILLG
                     RFTESSFDPANFVFG"
     gene            3310..4647
                     /gene="trmFO"
                     /locus_tag="VF589_00420"
     CDS             3310..4647
                     /gene="trmFO"
                     /locus_tag="VF589_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX8256065.1"
                     /translation="MTEAVHIIGGGLAGSEAAWQLAEAGVRVRLSEMRGSGEMTAAHH
                     TDSLAELVCSNSFRSDDAEQNAVGLLHAEMRALGSLIMACGDAHRVPAGSALAVDREG
                     VAAAVTARIAAHPNIELVRERVDALPASGPTIVATGPLTAARLAESIGAATGSDALAF
                     FDAIAPIVYRDSIDMDICWMAARWDKGGKDYINCPMTREQYLAFHQGLLDGEKGEFKA
                     WEKDTPYFEGCMPVEVMAERGVDTLRYGPMKPVGLDDPRTGRWPYAVVQLRQDNALGT
                     LWNMVGFQTKLKHAEQVRLFRTIPGLEKAEFARLGGLHRNTFINSPQLLDGTLRLRSA
                     PHVRFAGQITGCEGYVESAAVGLLAGRFAAAELAGAELAPPPPTTALGALLGHITGGA
                     DAATYQPMNVNFGLFPPIEGRTKKAERKLLYTSRARADLGEWQRASAPAESVG"
     gene            complement(4811..5248)
                     /locus_tag="VF589_00425"
     CDS             complement(4811..5248)
                     /locus_tag="VF589_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX8256066.1"
                     /translation="MLRYLAGVASALLLVAAGIFIWKSQAEADDPVPPAPAPTEFVAP
                     LKQPAAAPPPSAPAPAKSSEQKRFARYDGNKDGTITRPEMLDTRRNGYAKLDTNGDGR
                     LSFEEWAVTTATKFAEADGDRSGGLNAREFLTTRRETSPKCKC"
     gene            complement(5310..5990)
                     /locus_tag="VF589_00430"
     CDS             complement(5310..5990)
                     /locus_tag="VF589_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX8256067.1"
                     /translation="MPAAGAGGGTGLDADRTLALTYVPARRRPGVEALWRLDLALGQV
                     LAGGRDPMLSRIKLVWWRDRLEELDRARAPAEPVLQALAEHVIGRGVAGAALAPMVEG
                     WAALLSEDPLTQADLDTYAGARGGLLFRHSAVLLGGEADGGVEQGGEAWALVDLARHS
                     NPVDAEAALAAAGRRLAAMRWPSALRPIGMLASLAARDVGRGGAELEPQGVPGRMVRM
                     LRHRLTGS"
BASE COUNT          982 a         2124 c         2035 g          937 t
ORIGIN      
        1 cccggatcag gtccgggatg acgattcaac tcggatcaaa accgacgctc ccgctgaaga
       61 tcccgcgcgc cacttcgttg tcgcctcgcg ccttttggtg tagtcttgcg cccgtcgaca
      121 ccccaggcga cgcccaaaat agcggggtag cagcatgtcg gactatggct acagcttcta
      181 cctctatcgc gagagcggct atttcgacat ctcgatcgat tattcgatcg acggctatca
      241 cgatcccgaa actttcgaat acttcggcga tacttcctgg gattacagca taaacggcgt
      301 cggcggcggc ggtggctacg gcgcggaatc gggcaacgcc ttcttcagca tggggttcgg
      361 cagcggcgag caggtcgagc atcacatatt cgaattcagc gcgcagaaca ccttcagcgc
      421 cgagtcgatc tcgttccggt tcgacatgct gctggcgggc gcggcgaccg ccaatctcgt
      481 catcgcgggc acggactata gcgaactgat cgtctccgga tcgggcgccg acagcctcag
      541 cggcgccggc ggccgcgatc atctcgacgc cggcgacggc gacgacagta tcgatggcgg
      601 cgacggcaac gacctgatcg acggcggcag cggcgccgac ctgatggccg gcggcgcggg
      661 cgacgaccga tatcatgtcg accgggccgc cgacagcatc gtcgagcttg ccgacggcgg
      721 cggcgatcac gtcctttcgc gcctgtcctg gaccttgagc gagcatgtcg aagcgctcac
      781 cctctccggg cccaccgcgc gccgcggcat cggcaacgcc tccgacaatc tgatctacag
      841 caacaacctg gcgaacaccc tcgctggcct cgagggcgac gacagcctca ccggccacgg
      901 cggggacgac ctgctccacg gcggcggcgg cggcgacaag ctgttcggcg gcctcggcct
      961 cgacacgctg aagggcggcg ccggcgacga cacctatttg gtcgaccagg cttccgacat
     1021 tctcctcgag gccgcggcaa gggcatcgat acggtcgaga tcttcttctc gatcccgagc
     1081 tacgcgctcg gcgccaatct cgagaacatg accttcaccg gcaatgccga gccgttccgc
     1141 gggttcggca acgtgctcga caatgtcatc gccggcgcgc gcggcgacga ccggctgttc
     1201 ggcatgcgcg gcaacgaccg gctcgagggg cgggtcgggg acgacatgct ggtcggcggc
     1261 ggcgggaccg acagcttcgt cttcgccacc ggccagggca gggacaggat cgccgacttc
     1321 gatgcgctcg gcctcgagac cgtggcgatc gccggcggca gccacttcga caccttcgac
     1381 gagatcatgg cggcggcgcg gtcggtgggg ccggacggcg gcagcacgct gctgaaattc
     1441 ggcgccggaa gcaccctgct gctgaccaac gtcgccatgg acgatctcac cgccgccaat
     1501 ttcggcctct agcgcccacc ccaaacccaa ggcagagccg gcgtcgattt ccgcttgggc
     1561 ggggatggcg ccaagtgcgc ggcatgaacg acaaggtcca catcatcggc ggcgggcttg
     1621 cgggctcgat gaagtcgcgc gccggccggg ggtgacggtg ctaccccgaa tgcacagccc
     1681 ggcggagtga tgtcacggcc gcgaccggca gcgcctgccg gcaagaattg acgcacttca
     1741 tgtgccgcaa cgagacgccg ggcgcacgcg gcgggtattc gtcggcggat gagttgccgc
     1801 aggcgtgagc gatccatagg ccgtacatgt cagtcggttc gcgaagcttg gagtttgcgt
     1861 catgtccagt ccctatttta tacgcggctt cactcccgat ttcgatcgcc agtctggcac
     1921 attgaagatc gccgtcagct acgtcatcca gccttactcc aacccgttca cccacttcac
     1981 ctccgcgcgc actagctgga gctacgacat ccgcggcacg aacatggcgg tcaacggcag
     2041 cggggaaggt tcggctgctc ccacgattcg tacggcagtg gaggtgcagc agacggccgg
     2101 cccgcagagg ttcagcttct cgttccacgc ggagaacaca tattcgggtg ccgccgtcga
     2161 cctggcgctc aatcttctca tcgatgtcta ttccgcaggc gacgtgatca tcggcgggac
     2221 cgcgggcgac gacctgatcg tcgccggcgt cggcgacgac gtcctgcgcg gcggctcggg
     2281 cagcgattat ctcgcgggcg acagcggcga cgacatcctc gtcggcggcg gcgatcgtgc
     2341 gggcgaccgt ctcgacggcg gagcaggcgc cgaccgcatg tacggcggct tcggcaacga
     2401 tcgatatcgc gtgaacagcc gcggcgaccg agtcatcgag cgcgccgacg ccgaccccaa
     2461 caacaaagac atcgtcgaag cgagcgtcga ccatgtgctc gccgcaaatg tcgaggcgct
     2521 gtggctcctg ggagccgcag gcctgaacgg cggcggtaat gccctcgaca accaattggg
     2581 tggcaacgac ggcgcaaacc gcctgtccgg cttcggcggg cgcgacgagc tccacggcaa
     2641 gggcggggcg gacattctgc tgggcggtgc gggcaacgac gtcctgtacg gcggcgagga
     2701 cttggatgag gaccgcctcg cgggcggctc cggcgacgac cattattacg gcctcgccgg
     2761 cgatcggatt gtcgaagcag cgggaggcgg caccgacttc gtctggaccc acgaggcatt
     2821 cttcgccctt cccgaccacg tcgaaaatct tgacgccagc ggaaggggaa cgggatcctt
     2881 caggggcctg ggcaattccg gcgacaacgt catcgcgggc agcttcttcg ccgacgatcg
     2941 cctcgccggt cgtggcggcg ccgacaccct ttccggcagc ggcgggcggg acgtccttgc
     3001 cggcggcggc ggcaccgaca cgttcggatt tgccctcgag tcggacgtgg acaaggtgac
     3061 cgacttccgg gccggcgccg acggcgagcg cctggcgctc gatctcggct ccgccttcga
     3121 cacgatcgag gaggtcatgg cagtggcggt attcagcggc gaccgggtgg tgttcgactt
     3181 cggcgacggc gaccggctca tcctgctcgg ccgctttacc gagagcagct tcgacccggc
     3241 gaacttcgtc ttcggctgag cccggccttt cttcttccgt cacggcggcc gaggccctaa
     3301 cagccgccca tgacggaggc ggttcacatc atcggcggcg ggcttgcggg ttcggaagcc
     3361 gcctggcagc tggccgaagc gggcgtcagg gttcggctca gcgaaatgcg cggctcgggc
     3421 gaaatgaccg ccgcgcacca caccgactcg ctcgccgaac tggtctgttc gaacagcttc
     3481 cggtcggacg atgccgagca gaatgcggtc gggctgctcc atgccgagat gcgcgcgctc
     3541 ggttcgctga tcatggcgtg cggcgacgcg caccgggtcc cggccggatc ggcgctggcg
     3601 gtcgaccgcg aaggcgttgc ggcggcggtg accgcgcgga tcgccgccca tcccaatatc
     3661 gaactggtgc gcgagcgcgt cgacgcccta cccgcctccg gccccaccat cgtcgcgacc
     3721 gggccgctca cggccgcgcg actggcggag tcgatcggcg ccgccaccgg ctcggacgcg
     3781 ctcgccttct tcgatgcgat cgcgccgatc gtctatcgcg acagcatcga catggacatc
     3841 tgctggatgg ccgcccgctg ggacaaaggc ggcaaggact atatcaactg cccgatgacc
     3901 agggagcagt atctcgcctt ccaccagggc ctcctcgacg gcgagaaagg cgagttcaag
     3961 gcgtgggaga aggacacgcc ttatttcgaa ggctgcatgc cggtcgaagt gatggcggag
     4021 cgcggcgtcg acacgcttcg ctacggtccg atgaagccgg tcggcctcga cgaccccagg
     4081 accggccgct ggccctatgc ggtggtgcag ctgcggcagg acaatgcgct cggcacgctg
     4141 tggaacatgg tcggcttcca gaccaaattg aaacatgccg agcaggtgcg gctgttccgg
     4201 accatccccg gcctcgagaa agccgagttc gcgcgcctgg gcgggctcca ccgcaacacc
     4261 ttcatcaact cgccccaatt gctcgacggt acgctgcgcc tgcggagcgc gccgcacgtc
     4321 cgcttcgccg gccagatcac cggctgcgaa ggctatgtgg aatcggccgc ggtcggcctg
     4381 ctggcgggac gcttcgccgc cgccgagctg gccggcgccg agctcgcccc gcccccgccg
     4441 accaccgctt tgggggcgct gctcgggcat atcaccggcg gcgccgacgc ggccacctac
     4501 cagccgatga acgtcaattt cggcctgttc ccgccgatcg agggccgcac caaaaaggcc
     4561 gagcgcaagc tcctctacac ctcccgcgcc cgcgccgacc tcggcgaatg gcagcgcgcc
     4621 tccgcgccgg cggagagcgt cggctgagcg gcggaagccg ggagccggga gcatcgactg
     4681 atccgactag atcgtcaccc cggacttgat ccggggtctc ccttcttctt ttcgccgccc
     4741 cagaagaaga agaagaaggg agatgccgga tcaagtccgg catgacgact gctccactcc
     4801 taacctgccg tcagcacttg cacttgggcg aggtctcccg ccgcgtcgtc aggaactcgc
     4861 gcgcgtttag gccgccggag cggtcgccgt cggcttcggc gaacttggtg gcggtggtca
     4921 ccgcccattc ctcgaagctc agccgcccgt cgccattggt gtcgagcttg gcatagccgt
     4981 tgcggcgcgt gtccagcatt tcggggcggg tgatggtgcc gtccttgttg ccgtcatagc
     5041 gggcgaagcg cttctgctcg gacgacttgg cgggcgcggg cgcggacggc ggcggcgcgg
     5101 ccgcgggctg cttcaagggc gcgacgaatt cggtcggcgc cggcgccggc ggcaccggat
     5161 cgtccgcctc cgcctggctc ttccagatga agatgccggc ggcgaccagg agcagggccg
     5221 aggccactcc agccaggtaa cggagcatgg cagttccttt cactaacgag cctcagccct
     5281 agcgggatcg cccgccccgt ccaagcgcct caggagccgg tgagccggtg gcgcagcatc
     5341 cgcaccatgc ggcccggcac gccctgcggc tcgagttcgg cgccgccgcg cccgacgtcg
     5401 cgcgcggcga ggctcgccag catcccgatc ggccgcagcg ccgaaggcca gcgcatcgcc
     5461 gccagccgcc gcccggccgc ggccagcgcc gcctcggcat cgaccgggtt ggagtggcgc
     5521 gcgaggtcga ccagcgccca cgcctcgccg ccctgctcga ccccgccgtc ggcttcgccg
     5581 ccgagcaaga ccgctgagtg gcgaaacagc agtccgcccc tcgcccccgc ataggtgtcg
     5641 aggtcagctt gggtgagcgg atcctcgctc agcaaagccg cccagccctc caccattggc
     5701 gccaaggcgg cgccggcgac gccgcgcccg atcacatgct cggccagcgc ctgcagcacc
     5761 ggctcggcgg gcgcccgcgc gcggtcgagt tcttcgagcc ggtcgcgcca ccagacgagc
     5821 ttgatccggc tcagcatcgg atcgcgcccg ccggccagca cctggccgag cgcaaggtcg
     5881 agccgccaca acgcttccac cccgggccgc cggcgggccg gaacataggt cagcgccagc
     5941 gtgcggtcgg cgtcgagccc ggtgccgcct ccagctcccg ccgccggcat ggcgtcagcc
     6001 gcgataggtg accttcttcg ccgccgcgac cacatcggcg accttgatca gcgccatctt
     6061 ctcgagattg gcggcata
//
LOCUS       DASVRK010000010         8501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_437822, whole genome shotgun sequence.
ACCESSION   DASVRK010000010 DASVRK010000000
VERSION     DASVRK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437822"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VF589_00435"
     CDS             <1..698
                     /locus_tag="VF589_00435"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8256068.1"
                     /translation="HPLVEGTMELPDLIAIAKRLGGRKSRADATRYPECLAELSADEA
                     RALGEEYLERTRVQRKTGKPFFIDKLPNNWAHAGFIRLILPNAKIVDARRHPLACCFS
                     NFKQHFARGQGFSYDLAELGRYYRDYVRLMRHHDAVLPGHVHRVVHEALVEDPEAELR
                     RLLAYLGLPFDAACLRFHENERAVRTASSEQVRRPINREGLDQWKPFEPWLGPLKEAL
                     GPVLDAYPEAPSD"
     gene            829..3312
                     /locus_tag="VF589_00440"
     CDS             829..3312
                     /locus_tag="VF589_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8256069.1"
                     /translation="MRALSTSLTSRTVKSVSGALLATTMLCSTGTAFAQDAAPPADPV
                     GTGVAAGDATLTDDPAASSRGQSEEAEIIVTAQKRVENLQDVPIAITAFGTEKLDQLQ
                     VSDFDDYARYIPSISSQSSGPGFANVYFRGVASGENANHSTSLPSVGTYLDEQPITTI
                     TGALDIHIFDAARVEALAGPQGTLYGASSQAGTVRIITNKPDTSGFYGEANVEVNSVA
                     HGGIGYAGEAFVNAPLSDRAAVRVVGWYRKDAGYIDNVVGTLNFPTSGISFSNAGLVE
                     EDYNDVETYGGRAALKIDLDDNWTITPQVMGQKQIANGSFAQESGLPELQVQQFNPER
                     SNDRWIQAALTVEGKIGIFDVTYAGSYLTRRVDGATDYVDYAYFYDKLAGYGAYFYDN
                     DGDLVNPNQYIEFTDRYTKQSHELRFASPGTERVRFIGGLFYQRQSHNIEQNYIIDNI
                     ADAITVPGTESNIWLTKQIRVDRDYALFGEMTFDMTEKLSFTLGSRIYRYDNSLVGFF
                     GYSDGYSSKTGVAACFGPAVVNGSPCTNLDKRTADTDFIHKINTTYKFNDDALIYGTV
                     SRGFRPGGINRRGTLPPYAADFIDNYELGLKTSWLDNRLRFNAAIYQLDWTDIQLSFL
                     GENGLSEVRNAGNARIRGAEVDVFARLAPGLTLSAGAAYNDAKITKDFCLVANPQFDC
                     TLDPGDGRENELLAPAGTRLPVTSKFKGNLLARYEFPIGPGEGHFQAGLIHEGRRTSD
                     LRLLEREIVGDLDSYNTVDLSAGFKTSTWTAEVFVRNLFDELGQTARGIQCLETVCGD
                     PGGDTLIGPKIYTYVTQPRTIGLKIGTKF"
     gene            3319..4656
                     /locus_tag="VF589_00445"
     CDS             3319..4656
                     /locus_tag="VF589_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8256070.1"
                     /translation="MSEAAEAGPSPPRRVLGIWMCLALVMGNMIGSGVYLLPASLAPL
                     GWNGVFGWLVTIAGALCLAYIFASLSRVLPRAGGPYAYSREAFGPFAGFLVAWSYWCS
                     LWVGNVAIATGVVAPLSTLVPGVEENSVLVTCGMIWAMTAINCYGAQLAGRVQLVLTV
                     IKALPLAAVVLLAAMVIGGGAALPPLRAEDIRLTGEASIAAAGALTLWAFLGLESATV
                     PAEKVQDPARTIPRATLIGTAVTGAIYLFACSSVALLLPPEQAAQSGAPLADFVGRYW
                     GGAAGQWIAFFAVISAFGALNGWILLQGEMPWAMARDGVFPRFLAHTSRRGTPVRAHL
                     TSSVLLTVVLMLNYTKSMAELFTFLILLATTASLVPYLVCALAALKLRPAAGRWLPIV
                     AVAGAIYSLWAIWGAGTDAIVPWLILIAIGIPVYWLMRRSAAANQPPDERAVG"
     gene            complement(4612..6210)
                     /locus_tag="VF589_00450"
     CDS             complement(4612..6210)
                     /locus_tag="VF589_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8256071.1"
                     /translation="MTKNYDAIIIGAGHNGLVCAFYLARAGLKVRIVERRGVVGGAAV
                     TEEFHPGFRNSVASYTVSLLQPKVIADMKLADHGYKVIERPISNFLPQEDGGYLKLGG
                     GLAATQAEFRKFSDKDAETLPAYYAALENVADVLRDLALRTPPNLGGGLKTLIDGARQ
                     GRRLAGMPLDQQRDVLDLFVKSARSFLDSWFESEPVKAAFGFDAVVGNYASPDTPGSA
                     YVLLHHVFGEVNGKRGAWGHCVGGMGAITEAMARICRAAGVEISLEAPVARVLVDGGK
                     AAGVRLESGEEIAAATVIANVGPKLLYERMMDPADLTPEFHRRIKAFKAGSGTFRMNV
                     ALSELPRFTALPEPGEHLQSGIIIAPTLDYMDRAFLDAKAYGMSRRPIVEMLIPSIID
                     DSLAPPGSHVASLFCQQFAPELEWDAHRDAAADLIIDTVDEYAPGFKASVIGRMSLSP
                     LDLERKFGLVGGDIMHGNMTLDQLWSARPVLGNAAYRGPVKGLYMCGAGTHPGGGVTG
                     APGHNAAREILADRSFVGRLVRRG"
     gene            complement(6318..7553)
                     /locus_tag="VF589_00455"
     CDS             complement(6318..7553)
                     /locus_tag="VF589_00455"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX8256072.1"
                     /translation="MTQPQRAEPLRNSDPDEGWSLPAWTYEDPEFFRAETERVMRPSW
                     QVVCHVSDVAGVGDWHTLEFLGESILVVRGKDMAVRAFTNVCRHRGSRIVDGASGCAK
                     KLVCPYHAWTYELDGRLAGVPGKSGYAGLDTSQHGLAPVELEIWRGFIFVRLGGPLHR
                     PADGPPPRSGEELPSVASMMAPYEEMVAPYRFEDLRAEGRVTLRPRAVNWKNVADNYS
                     DGLHIPIAHPGLTRLFGRGYGIEAEEHVDRMWGAMIDRPSANWSERMYQAILPPVPHL
                     PPEAQRYWLYFKLWPNVAFDIYPDQVDFMQFLPVSPTETLIREISYALPDDRRQMKAA
                     RYLNWRINRQVNAEDTELIARVQAGMQSQSFTVGPLSEAEVCLRSFCRKMRGLIPEAR
                     QHRPPAPGWSTRSPVQAAN"
     gene            7600..8196
                     /gene="betI"
                     /locus_tag="VF589_00460"
     CDS             7600..8196
                     /gene="betI"
                     /locus_tag="VF589_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HEX8256073.1"
                     /translation="MSSREKADVRRQSLIEAAAASLAERGVAGTSVRTICARAGVSPG
                     LLRHYFDGIDALVAETYRDVTARVAAALAAAVEAAGEAPRARLVAFVTASFRPPIADP
                     SLLATWLAFWSLVNTDAAIADIHRRTYRGYRADLEALLAACGVPAADTAPVAIALTAL
                     VDGLWLELSLDPSTFTPDQASAMAVRWLDALVAGQGEG"
BASE COUNT         1363 a         2877 c         2817 g         1444 t
ORIGIN      
        1 gccatccgct ggtcgaggga acgatggagc tgcccgatct gatcgccatc gccaagcgcc
       61 tcggcggccg caagtcccgc gccgatgcga cccggtatcc ggagtgcctg gcggagcttt
      121 cggcggacga ggcgcgggcg ctgggcgagg aatatctcga gcgcacgcgg gttcagcgca
      181 agaccggcaa gcccttcttc atcgacaagt tgccgaacaa ttgggcccat gccggcttca
      241 tccggctgat cctgccgaac gcgaagatcg tggatgcccg ccgccacccg ctcgcctgct
      301 gcttttccaa cttcaagcag catttcgcgc gcggccaggg cttcagctac gacctcgccg
      361 agctcggccg ttactatcgc gattatgtgc ggctgatgcg ccatcatgac gcggtgctgc
      421 ccgggcatgt ccaccgcgtc gtccacgaag ccctggtcga ggatcccgaa gccgagcttc
      481 ggcggctgct cgcctatctc ggcctgccgt tcgacgccgc ctgcctgcgc ttccacgaga
      541 atgagcgggc ggtgcggacc gccagttcgg agcaggtgcg gcggccgatc aaccgcgagg
      601 gcctcgatca gtggaagccg ttcgagccgt ggctcggccc gctcaaggaa gcgctggggc
      661 cggtgctcga cgcctatccg gaggcgccgt cggactgacg tggccacgtg gcaggaaagc
      721 cacatcggcg cgcgaaccgt gactggttcg ttacagacgc gttattgaaa ttgacgcttc
      781 ccgaaaagcg acacaggttg cccccggggc ctacttcgtg ggggatatat gcgcgcgcta
      841 tcgaccagcc tgaccagccg cacggtcaaa tccgtctcgg gcgccttgct tgcgaccacg
      901 atgctgtgct cgacgggcac cgccttcgcg caggatgccg caccgccggc cgatccggtg
      961 gggaccggcg tcgccgccgg agatgccacc ctgaccgacg atcccgccgc cagcagccgc
     1021 ggccagagcg aggaagccga aatcatcgtc accgcgcaaa agcgggtcga gaatctgcag
     1081 gacgtgccga tcgcgatcac cgctttcggg accgagaagc tcgaccagct gcaggtctcc
     1141 gacttcgacg attatgcgcg ctatattccc agcatctcgt cccagtcgtc cgggccgggt
     1201 ttcgccaacg tttatttccg cggcgtcgcg agcggcgaga atgccaacca ctcgacctcg
     1261 ctgccgagcg tcggcaccta tctcgacgag cagccgatca cgaccatcac cggcgcgctc
     1321 gacatccaca tcttcgacgc cgcccgggtc gaggcgctgg ccgggccgca aggtacgctc
     1381 tacggcgcgt cgagccaggc cggcaccgtg cggatcatca ccaacaagcc cgacacgtct
     1441 ggcttctacg gcgaggccaa tgtcgaggtg aactcggtcg cgcacggcgg catcggttat
     1501 gccggcgagg ctttcgtcaa cgctccgctg tcggatcgcg ccgcggtccg cgtcgtcggc
     1561 tggtaccgca aggatgcggg gtatatcgac aacgtcgtcg gcacgctgaa ctttcccact
     1621 tcgggcatct cgttcagcaa tgcgggcctg gtcgaggaag attataacga cgtcgagacc
     1681 tatggcggcc gtgccgcgct caagatcgat ctcgacgaca attggacgat cacgccgcag
     1741 gtgatgggcc agaagcagat cgcgaacggc tccttcgcgc aggaatccgg ccttcccgag
     1801 cttcaggtgc agcagttcaa tcccgagcgc tccaacgacc gctggatcca ggcggcgctg
     1861 acggtcgagg gcaagatcgg cattttcgac gtgacctatg ccggctccta tctgacgcgc
     1921 cgggtcgacg gggcgaccga ctatgtcgat tacgcctatt tctacgacaa gctggcgggc
     1981 tacggcgcct atttctacga caatgacggg gatctggtga accccaatca gtatatcgag
     2041 ttcaccgacc gttataccaa gcagagccac gagctgcgct tcgcctcgcc gggcaccgag
     2101 cgggtgcgct tcatcggcgg cctcttctac cagcgccagt cgcacaatat cgagcagaat
     2161 tacatcatcg acaacatcgc cgacgcgatc accgtgccgg gcacggaaag caacatctgg
     2221 ctcaccaagc agatacgcgt cgaccgcgac tatgcgttgt tcggcgagat gacttttgac
     2281 atgaccgaga agctcagctt cacgctcggc agccgcatct accgttacga caattcgctg
     2341 gtcggcttct tcggctacag cgacggctac agcagtaaga ccggggtcgc cgcctgcttc
     2401 gggcccgccg tcgtcaacgg ctcaccctgc acgaacctcg acaagcgcac ggccgacacc
     2461 gatttcatcc acaagatcaa cacgacctac aagttcaacg acgatgcgct catctatggg
     2521 accgtatcgc gggggttcag gccgggcggc atcaaccggc gcggcacctt gccgccttat
     2581 gcggccgatt tcatcgacaa ttacgagctc ggcctcaaga cgagctggct cgacaaccgg
     2641 ctgcgcttca acgccgccat ctatcagctc gactggaccg acatccagct ctccttcctc
     2701 ggcgagaacg gcctttcgga agtgcggaac gccggcaatg cgcgcatccg cggcgccgag
     2761 gtggatgtgt tcgcgcggct ggcgccgggc ctgaccctgt ccgccggcgc ggcctataac
     2821 gacgccaaga tcaccaagga cttctgcctg gtcgccaacc cgcagttcga ttgcacgctc
     2881 gatccgggcg acgggcgcga gaacgagctg ctcgctcccg ccggcacgcg gctgccggtc
     2941 acctcgaagt tcaagggcaa tctgctcgcc cgctacgagt ttccgatcgg gccgggggaa
     3001 ggccatttcc aggccggtct gatccacgaa ggccgccgca ccagcgacct tcgcctgctg
     3061 gagcgcgaga tcgtcggcga cctcgattcg tacaatacgg tcgatctcag cgccggcttc
     3121 aagacctcga cctggacggc ggaagtgttc gtgcgcaacc tgttcgacga gctcggccag
     3181 accgcgcgcg gcatccagtg cctcgagacg gtgtgcggcg accccggagg cgataccctg
     3241 atcggcccga aaatctatac ctacgtcacc cagccgcgga ccatcgggct gaagatcggc
     3301 acgaaattct agcttgagat gagcgaggcg gcggaagcag gaccaagccc gccgcgccgc
     3361 gtgctcggca tctggatgtg ccttgcgctg gtcatgggca acatgatcgg atcgggcgtc
     3421 tatctgctcc cggcgagcct ggcgccgctc ggctggaacg gcgtgttcgg ctggctggtg
     3481 acgatcgcag gcgcgctctg cctcgcttat atattcgcca gcctgtcgcg ggtcctgccg
     3541 cgggcgggcg gcccttacgc ttattcgcgc gaggcgttcg ggccgttcgc cggcttcctc
     3601 gttgcctgga gctattggtg ctccttgtgg gtcggcaatg tcgccatcgc caccggcgtg
     3661 gtggcgccgc tgagcacgct ggtgccgggg gtcgaggaga atagcgtcct cgtcacctgc
     3721 gggatgatct gggcgatgac cgccatcaac tgctacgggg ctcagctggc cgggcgggtc
     3781 cagctcgtcc tcaccgtcat caaggcgctg ccgctggcgg cggtggtgct gctggccgct
     3841 atggtgatcg gcggcggcgc ggcgctgccg ccattgcggg ccgaggacat ccgcctcacc
     3901 ggcgaagcca gcatcgccgc cgccggcgcg ctcacgctct gggcctttct cgggcttgaa
     3961 tcggctaccg tgccggccga gaaggtccag gatccggccc gcactattcc gcgcgccacc
     4021 ctgatcggca ccgcggtcac gggcgccatc tacctgttcg cctgctcctc ggtggcgctg
     4081 ctgctgccgc cggagcaggc agcgcaatcg ggcgccccgc tcgccgattt cgtcggccgc
     4141 tattggggcg gcgcggccgg gcagtggatc gcctttttcg ccgtgatcag cgcgttcggc
     4201 gccttgaacg gctggatcct gctgcagggc gagatgcctt gggcgatggc ccgggacggc
     4261 gtgttcccgc gcttcctcgc ccacacctcg cggcgcggca caccggtgcg ggcgcacctc
     4321 acctcgagcg tgctgctgac cgtcgtgctg atgctcaact acaccaagtc gatggccgag
     4381 ctgttcacct tcctgatcct gctcgccacc actgcctcgc tggtccccta tctggtctgc
     4441 gcgctggcgg cgctgaagct gcgcccggcc gcgggacgat ggctgccaat cgtggcggtc
     4501 gccggcgcga tctactcgct atgggcgatc tggggcgcgg gcaccgacgc gatcgtgccg
     4561 tggctgatcc tgatcgcgat cggcattccg gtttactggc tgatgcggcg ctcagccgcg
     4621 gcgaaccagc cgcccgacga acgagcggtc ggctaggatc tcgcgcgccg cattgtggcc
     4681 cggcgcgccg gtgacgccgc cgcccggatg ggtgccggcg ccgcacatat acagcccctt
     4741 caccggaccg cgataggcgg cgttgccgag caccggccgc gccgaccaga gctggtcgag
     4801 ggtcatgttg ccgtgcatga tgtcgccgcc gacgaggccg aacttgcgct cgaggtcgag
     4861 cggcgacagg ctcatccggc caatgaccga agccttgaag ccgggcgcat attcgtcgac
     4921 cgtgtcgatg atcaggtcgg ccgcggcgtc gcgatgcgcg tcccattcga gctctggcgc
     4981 gaattgctgg cagaacaggc tggcgacatg gctcccgggg ggcgccaaac tgtcgtcgat
     5041 gatcgacggg atcagcatct cgacgatcgg ccggcgcgac atgccatagg ctttggcatc
     5101 gaggaaggcg cggtccatat aatcgagcgt cggcgcgatg atgatgccgg actggagatg
     5161 ctcgccgggc tccggcagcg cggtgaagcg cggcagctcc gacaaggcaa cgttcatccg
     5221 gaaggtgccg gagcccgcct tgaacgcctt gatgcggcgg tggaattcgg gggtcaggtc
     5281 cgccgggtcc atcatccgct cgtagagcaa tttggggccg acattggcga tcacggtggc
     5341 ggcggcgatc tcctcgccgc tctccagccg cacgccggcg gccttgccgc catcgaccag
     5401 tacccgagct accggcgcct cgaggctgat ctcgaccccg gcggcgcggc agatcctcgc
     5461 catcgcctcg gtgatcgcgc ccatcccgcc gacgcaatgg ccccaggcgc cgcgcttgcc
     5521 gttcacctcg ccgaacacgt ggtggagcag cacataggcg ctgccgggcg tgtcggggct
     5581 ggcataattg ccgaccaccg cgtcgaagcc gaacgccgcc ttgaccggct cgctctcgaa
     5641 ccaagaatcg aggaagctgc gcgcgctctt gacgaacagg tcgagcacgt cgcgctgctg
     5701 gtcgagcggc atgccggcga ggcggcggcc ctggcgggcg ccatcgatca gggtcttgag
     5761 ccccccgccg agattgggcg gggtcctgag cgcaaggtcg cgcagtacgt cggcgacatt
     5821 ctccagcgcc gcataataag cgggcagggt ctcggcgtcc ttgtcggaga atttgcggaa
     5881 ctcggcctgc gtcgcggcga gcccgccgcc gagcttcaga tagcctccgt cctcctgcgg
     5941 caggaagttg gagatcggcc gctcgatcac cttatagccg tgatcggcca gcttcatgtc
     6001 ggcgatcact ttgggctgga gcaggctcac cgtgtagctc gcgacgctgt tccggaagcc
     6061 ggggtggaat tcctcggtca ccgccgcgcc gccgaccacc ccgcgccgct cgacgatccg
     6121 caccttgagg ccggcgcgcg cgaggtaaaa ggcgcagacg aggccgttat gcccggcccc
     6181 gatgatgatc gcgtcataat tttttgtcat cgggactcct tgatcgattt ccgttcgggc
     6241 tgagcttgtc gaagccttct acttccttcc caccggttga aggagggcag ggcttcgaca
     6301 ggctcagccc gaacggatca gttggcggcc tgcacgggtg aacgcgtact ccaccccggc
     6361 gccggcgggc ggtgctggcg cgcttccggg atcaggccgc gcatcttgcg gcagaagctc
     6421 cgcaggcaca cctccgcctc actgagcggc ccgacggtga agctttgcga ctgcatcccc
     6481 gcctgcaccc gcgcgatcag ctccgtgtct tcggcattga cctggcggtt gatgcgccag
     6541 ttcaaatagc gcgccgcctt catctggcgg cggtcgtccg gcagcgcgta gctgatctcg
     6601 cggatcaggg tctcggtcgg cgagaccggc aggaactgca tgaaatcgac ctggtcggga
     6661 tagatgtcga aggcgacgtt cggccacagc ttgaaataga gccagtaacg ctgcgcctcg
     6721 ggcggcaaat gcggcaccgg cggcaggatc gcctggtaca tgcgctccga ccaattggcc
     6781 gagggccggt cgatcatcgc cccccacatg cggtcgacat gctcctccgc ctcgatgccg
     6841 tagccgcgcc cgaacaggcg ggtgaggccg ggatgcgcga tcgggatgtg gagcccgtcc
     6901 gaataattgt cggcgacatt cttccagttg accgcgcgcg ggcgcagcgt gacgcggcct
     6961 tccgcgcgca gatcctcgaa ccggtagggc gcgaccattt cctcgtaggg cgccatcatc
     7021 gacgcgaccg agggaagctc ctccccggaa cggggagggg gaccgtccgc aggacggtgg
     7081 agggggcccc ccagccgcac gaagatgaag ccccgccaga tttcgagctc gaccggcgcc
     7141 agaccatgct ggctggtgtc cagtccggca tagccgctct tgcccggcac gccggcgagg
     7201 cggccgtcga gctcgtaggt ccaggcatga taggggcaga ccagcttctt ggcgcagccc
     7261 gacgcgccgt cgacgatgcg cgatccgcgg tggcggcaga cgttggtgaa ggcgcgcacc
     7321 gccatgtcct tgccgcgcac gaccaggatg ctttcgccga ggaattcgag cgtgtgccag
     7381 tcgcccacgc cggcgacgtc gctgacgtgg caaaccacct gccagcttgg gcgcatcacg
     7441 cgctcggtct cggcgcggaa gaattcggga tcctcatagg tccaggcggg caggctccag
     7501 ccctcgtcgg gatcgctgtt gcggagcggt tcggcgcgct gcggctgggt catcggggcc
     7561 tcctttatac gatcgtacaa taagtgtatg ctgcccgcaa tgagcagccg ggaaaaagcg
     7621 gatgtgcggc gccagtcgct gatcgaggcg gcggcggcct cgctggcgga gcggggcgtc
     7681 gccggcacgt cggtgcgcac gatctgcgcg cgggcgggcg tgtcgccggg cctgctgcgc
     7741 cattatttcg acggcatcga cgcgctggtg gcggagacct atcgcgacgt gaccgcgcgc
     7801 gttgcggcgg cgctggccgc cgcggtggag gcggcggggg aggcgccgcg cgcccggctg
     7861 gtggcgttcg tcaccgcgag cttccgcccg ccgatcgcgg acccgtcgct gctcgccacc
     7921 tggctggcct tctggagcct ggtcaacacc gacgcggcca tcgccgacat ccaccgccgc
     7981 acctatcgcg gctaccgcgc ggacctcgaa gctttgctcg ccgcctgcgg ggtgccggcc
     8041 gccgacaccg cccccgtggc gatcgcgctc acggcgttgg tggacggcct ctggctcgaa
     8101 ctctcgctcg acccttccac cttcaccccc gaccaagcca gcgccatggc cgtccgctgg
     8161 ctcgatgcgc tggtggcagg gcagggggag ggctgaggca ggggtggcgg gctgacgcgt
     8221 cgaaccgaac cactccgttc gtgtcgagcg aagtcgagac atcctttcga gcgaagcgag
     8281 agctggagga tatcaaggcc tcggctccgc tcggcagggt ccctcgactt cgctcgggat
     8341 gaacgggggg aggtctcagc cgattggctc aaatctcccc gctcatcctg aggagggact
     8401 gagccccggc gaaggcccgt ctcgaaggac taccgtgcgt ccttcgagac gccgcttcga
     8461 cttcgctcag cggctcctca ggatgagcgg ttaagttcat a
//
LOCUS       DASVRK010000011        17518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_64868, whole genome shotgun sequence.
ACCESSION   DASVRK010000011 DASVRK010000000
VERSION     DASVRK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 17518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17518
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64868"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF589_00465"
     CDS             complement(<1..278)
                     /locus_tag="VF589_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256074.1"
                     /translation="MRMDQEVSSPAVRRRSLLSLLFLPLLAFIGGIAAMAWLLANWSA
                     AAGFLGLQPAAPPARAAAQVRPQPQPDPFAGGATLAAEQPQRLVIDPE"
     gene            complement(275..991)
                     /locus_tag="VF589_00470"
     CDS             complement(275..991)
                     /locus_tag="VF589_00470"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX8256075.1"
                     /translation="MKRKLLILRPQAGAERTARRIAELGVDPVVAPLFEVVPLAWQAP
                     DPAAVEAVMLTSANAASHAGASLSPFLAHPCYAVGEASAAAARAAGFGDVRIGRGDGK
                     RLLDRLAADGIERVLHLCGRDHLDLERPRLRLVRRFVYAAEASETLPPDAAAALRAGA
                     VALIHSPRSGALFRGLVEAAGLDPARIAVAAISPAAAAAAGRRWSAVAIAPVPRDHAL
                     LEVAAKLCKGDAIEATESGA"
     gene            complement(988..1899)
                     /gene="hemC"
                     /locus_tag="VF589_00475"
     CDS             complement(988..1899)
                     /gene="hemC"
                     /locus_tag="VF589_00475"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX8256076.1"
                     /translation="MDTPLRIGTRGSPLALAQARLVAAALREAHGWGPERMEIVAITT
                     SGDMIQDRPLSEVGGKSLWTKELDAHLIDGRTDLSVHSMKDVETFRPDEFCIAAMLPR
                     ADARDRLLGAVSLEALPGWARVGTSSPRRTAQLLARRPDLNIVAFRGNVATRVRRLEL
                     GEADATLLAAAGLDRLGLGEIGTPLDDLLPAPAQGAIGVEVRAENKKVRALVAAISHA
                     ETHQCVSAERRLLEGLGGDCRSPVAALALSEGDIIHLRAELLTHDGGEVVQGETRFAA
                     GDVEAPLELAQHLLGRASPSLRALFAG"
     gene            1944..2978
                     /gene="tsaD"
                     /locus_tag="VF589_00480"
     CDS             1944..2978
                     /gene="tsaD"
                     /locus_tag="VF589_00480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX8256077.1"
                     /translation="MSLILGIESSCDETAAALVTSDRQVLAHRLAGQESAHRPYGGVV
                     PEIAARAHVEILPSLIEDVLREADLSLDEVDAIAATAGPGLIGGVMVGLVTAKGLALA
                     ADKPLIALNHLEGHALSPMLSAPDLDFPYLLLLVSGGHCQLLFVEGVGRYRRVATTID
                     DAAGEAFDKSAKLLGLGFPGGPAIERAAADGNPKAVRLPRPLLGSDEPHFSFAGLKSA
                     VLRARDAGLYSAEDIAASFQQAVVDCLVDRTARALNEAPGATALVVAGGVAANQSVRA
                     ALTSLASVHGLPFAAPPLWLCTDNAAMIAWAGALRFAEGLVDGLDAPARARWPLDPDA
                     ERARGAGVKA"
     gene            2981..3961
                     /locus_tag="VF589_00485"
     CDS             2981..3961
                     /locus_tag="VF589_00485"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX8256078.1"
                     /translation="MRLGVVGGGAWGTALAQVAASGGEETLLWAREPEVVHSINSRHE
                     NKSFLAGIPLNAAIHATGELADLKACDALLVVTPAQHTRKVLSAMARWTKPLILCAKG
                     MEEATTKLMHDVAREEHPGCAIAVLSGPTFAHEVAAGLPAAVTLAVEDRGVGERLRAR
                     LALPWFRPYLSDDIVGAEIGGAVKNVLAIACGVVEGRGLGQNARSALISRGFAEMTRF
                     GLARGGRAATLAGLSGLGDLVLTCSSASSRNFSLGKGLGEGWDPAELLADRHTVAEGA
                     YTAPVLKRAAEAAGIDMPIVAAVCALLSGEAAVDAVVERLLSRPLRSEGA"
     gene            complement(3987..4193)
                     /locus_tag="VF589_00490"
     CDS             complement(3987..4193)
                     /locus_tag="VF589_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEX8256079.1"
                     /translation="MPLFNRETMMAGRRPDHVKPPAYLSKSRPVPAPDPAEPDAAAEE
                     HGGPKGREPTRYGDWENKGICWDF"
     gene            4235..5176
                     /gene="htpX"
                     /locus_tag="VF589_00495"
     CDS             4235..5176
                     /gene="htpX"
                     /locus_tag="VF589_00495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX8256080.1"
                     /translation="MNHVKTLMLLAALTALFMALGYTLGGSGGALMALLFAAGMNLFT
                     FWNADKIVLRMHKAREVDARSSPELFGIVRELAARAGLPMPKVYIVDSPHPNAFATGR
                     NPKNAAVAATTGLLALLSRDEVAGVMAHELAHIRNRDTLIMTMTATIAGAISFLANFG
                     LFFRGGDDNRGNMLAMIAAVIVAPFAAMIVQLAISRTREYGADRGAAEISGNPRALAS
                     ALAKLQQGAARVPNPVAMRNPAAASLYIVPSATGRDSMFSTHPDTGNRIAALHAMADE
                     LGLASPPPALGRGAPPRSSVPKTRRSASALDPLGRGD"
     gene            5173..6438
                     /locus_tag="VF589_00500"
     CDS             5173..6438
                     /locus_tag="VF589_00500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX8256081.1"
                     /translation="MSREDPIGTASRRAALRLLDAVLRKGLPLEAALDTAARDLDRAD
                     DRGFAHAIAAEALRRLTDLDALIDSATKKPLPDDAKARFALRIALVQALAMETPHHAA
                     ISTVLPLVEGGPRKLVHGVFSALMRSGVTLPEVPSLSPQVEARWETAWGSAVARAAAR
                     AMAVPPPIDLVLAGAEAPEGLKGESFMPGHLRLARGTAIAGLTGYGSGDWWVQDVSAS
                     MPARLLGDGRGRSALDLCAAPGGKTMQLASAGFDVTAVDASESRLARLRENLERTHLS
                     AQIEAADVMNWQPADPVDAILLDAPCSATGIFRRHPDVLHRVRPRVIAELAAIQQAML
                     ARAAGWLKPGGTLVYSVCSLEPEEGEKIVEAFLGARPDYALDPVTSDELPPPMRPNDG
                     ILRILPGLYEEQGGADGFFIARFRRISGG"
     gene            6487..7149
                     /gene="rpe"
                     /locus_tag="VF589_00505"
     CDS             6487..7149
                     /gene="rpe"
                     /locus_tag="VF589_00505"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX8256082.1"
                     /translation="MQQPVRIAPSILSADFARLGEEVRAIDAAGADWIHVDVMDGHFV
                     PNLTIGPGVVKALRPHTAKTFDVHLMISPVDNFLAAFAEAGADIITVHPEAGPHLHRT
                     LQHIKLLGKKAGVSLNPATPAKMLDYVLEEVDLVLVMSVNPGFGGQSFIDSQLRKVEA
                     VRKQIDKTGLSIDLEVDGGIDPDTARRAIEAGADVLVAGTATFRGGADHYADNIRRLR
                     GS"
     gene            7146..8888
                     /locus_tag="VF589_00510"
     CDS             7146..8888
                     /locus_tag="VF589_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX8256083.1"
                     /translation="MSLLERIHEADGQQEIEPGKRLIRVGDDRGVSLAERLAYRLHRL
                     AWRTPFHSFRLRGRFPLKLLTVPKDPIAGDKAAGEAILAGNIVHRGASLPIDRLDFTD
                     PSIPAECTEYLQSFAWLRDLAAAATREKGARVAEKICQKWLSAYSASVSEPAWRADLW
                     GRRILFWTAYAPYILSSRDMVYRSAVLHTLARGARHLDRGADKATAGLPRIAAWCGVI
                     ASAIVVQGGPARLGTGEGGLRRALDASLHEDGGLISRSPLEQLALVELLSQLRAVYMS
                     ARRQMPEDLAEALSGSVAALLAVTLGDEALSSWQGGNMGSKRRLAAAIEGSGVTARPL
                     RQARGWGYQRLAAKKSVAVFDAAPPPASRALSGGCASTLAFEFSDGAERLVVNCGGVG
                     PRISALPTDLIRGLRTTAAHSTLILGDRNSTAIHEDGTLGKGVGQVELSRDETAGTTI
                     VEASHDGYVRRFGLVHQRQLTLTSDGLELRGEDRLLPEGRRRRSEPTAFAVRFHLAPA
                     LEVTSTADGQGALLRIRGGAVWQFRCRGGALAIEDSLSVDGEARPHSSLQLVITGETP
                     PAGMSISWLLKRAG"
     gene            9087..10676
                     /gene="purH"
                     /locus_tag="VF589_00515"
     CDS             9087..10676
                     /gene="purH"
                     /locus_tag="VF589_00515"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX8256084.1"
                     /translation="MSDVKITRALLSVSDKAGLAELGQALAGHGVELVSTGGTARALR
                     EAGLTVRDISDLTGFPEMMDGRVKTLHPKVHGGLLGVRDDPAHAAAMAEHGIGAIDFV
                     VVNLYPFAATVAKGAGRDEIVENIDIGGPSMVRSAAKNHAFVTIVTDPADYAELIETL
                     AAGGGMTSLDLRKRLAAKAFAATAAYDAAIASWFAFADQGQLFPDLLPLAMHKREELR
                     YGENPHQQAALYLPAGPHARGIAQARQVQGKALSYNNYNDADAALELVAELRGGDPAC
                     VIVKHANPCGVAAAGTLLQAYQQAFDCDPVSAFGGIIAVNRPLTRDAAEAMTRIFTEV
                     VIAPDANEEARAIFAAKKNLRLLLTGDLPDPARPGLALKSIAGGYLLQSRDNGRVERG
                     DLTVVTKRVPTDQELADCLFAWTVAKHVKSNAIVYAKDGATAGIGAGQMNRLESARIA
                     AWKAKDAAEKAGWPEPRTIGSAVASDAFFPFADGLLAAVEAGATAVIQPGGSIRDDEV
                     IAAADEAGLAMVFTGMRHFRH"
     gene            10738..12270
                     /locus_tag="VF589_00520"
     CDS             10738..12270
                     /locus_tag="VF589_00520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8256085.1"
                     /translation="MNVGDERSFSYWMEFAPVLDAPPLTSDATCDVVVIGSGIAGLST
                     AYELARFGRSVIVIDRGKIGWGMTARTTAHLATELDDFFSELIRVRGEDDARLWHDSQ
                     VTAVNRIEAICRDEGIDADFARLDGYLFPAEEAHRAELEEEYQACKAINVNVEWVDRA
                     PVPGKDTGPALRFPNQGRFHPTKYLAGLAGAIVARGGRIYADTAHASDQETDDGVEIT
                     TEAGTVIRASAAVFCTNSPTNDKVAIHAKQTPDRTYVVAGRVPKGSVPDALVWDTYEA
                     YHYVRIQPLSETEDLIIVGGGDHRTGEADDMDKRLDDLESWTREHYPSFTRADYRWSG
                     QVLEPMDFMPFSGRNPGNKNIYIHTGDSGQGITNGVAGSLTIMPLIIGEDSRYAPVLD
                     PGRKSFTSTSSIGEFVREQAGSAKNYMEHITGGEIASADELAPGEGGLMRKGLHKIAV
                     YRGEDGSVTQRSAVCTHLGCIVHWNNFEKCWDCPCHGSQFAPDGQVLNGPAAKPLAEV
                     EE"
     gene            complement(12227..13759)
                     /locus_tag="VF589_00525"
     CDS             complement(12227..13759)
                     /locus_tag="VF589_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8256086.1"
                     /translation="MTQPAPAGDDIAALARGGRTNFFGFLLRLAARLPFLFIAGRIYG
                     AEALGRFAYAILIVEFAAQIATLGLKRGLAQQLSSTDKPHVCIVWDAMLVAFLASAVA
                     TILLAIFPQAMFPNSPIVGLEWLLPLIVFAIAGADVALAALAYRHNVRASVTSRAIVE
                     PWTISIAAFLLSYLSSRDGLIIAYVLSMLAAFIAALWPLFRTYGAPTGWRPDPVTLWA
                     LARRNTPLAAADAIEWASRRIDIAILGLFFSPAVVGVYYVAQQVASLPQKLKTSFDPI
                     LSPVITHKLAEGDKAAVARQVRQVGFWIIAAQAAIALALGIPAEAVMGLVGAAFIGGT
                     GALAFLLAAEVVAATAAVSESALVYVARHRNLMISVFSIAVQAGLSVALILGMRALGW
                     PATFQAAGPAIALMLALGMSSVLKARLLCRILGAPVQGWRWPLIWAAAAATVVGYGFT
                     LLPRSLEFVELVVGIPVILVTFGAVLWTRGFTHEDRVLFRMKRGEEATLPPPQGALPP
                     GR"
     gene            complement(13756..16524)
                     /gene="polA"
                     /locus_tag="VF589_00530"
     CDS             complement(13756..16524)
                     /gene="polA"
                     /locus_tag="VF589_00530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX8256087.1"
                     /translation="MSSPHLYLVDGSSYIFRAYHRLPPLTNRHGLNVGAVYGYTTMLW
                     KLAESLHKEDGPTHLAVIFDASAKTFRNDMYSEYKANRPPPPPDLVPQFPLIRDATRA
                     FSLPCIEEDGLEADDIIACYSKSALKEGWKVTIVSSDKDLMQLIEPGLDMLDTMNDRR
                     IGRDYVIDKFGVPPEQLGEVLALMGDSVDNVPGVPGVGPKTAAQLIKQYGDVETLLAH
                     VDEIAKPKLKQNLTEHAAAARMSRELVRLVCDSPLPEPLDTLQMKGIPDGPLREFLED
                     QGFRSLLARLNGTSQVRAPSDSPSAAPVQREVRPEPKIDRSAYETVTDAAALDRWIAE
                     ASANGLVAVDSETDGYDCVTAKLVGVSLATECGKACYIPLEHGGHDLLSERPEQVPAE
                     LALSKLKPMLEDPAVLKVGHNFKFDWIVFDRRGIKVAPIDDSLVMSFNLDAGGLTSHA
                     MDDLAARHLDHQCLTYKELCGTGQKQIKFSQVPLGRATEYAAEDADVTLRLWKRFKTR
                     LPHERVTRIYEMVDRPLVPVVARMEREGVKVDRDELHKLSTEFNQQILELEERICGEA
                     GCKFTIGSPQQLGDVLFNQMQLKGGRKGKSGTWSTDVNELERLSREGVPIARLVLEWR
                     QLTKLKSTYTDALQQQINKETGRVHTSYSLSGAQTGRLSSTDPNLQNIPIRTEIGRRI
                     RDAFVAEPGHVVLAADYSQIELRLAAHMADVPQLRDAFAAGADIHNMTAQELFGEANR
                     DTRASAKTINFAILYGISRWGLAGRLGVTADEAQAIIDRYFDRFPGIRDYIHETLTGA
                     RETGFTTTLFGRKTHFPGLKSKVQHERQGAERAAVNAPIQGTSADIIKRAMVRMCPAL
                     EAEGLGSTRMLMQVHDELVFEVPEADVERAGAVIRRVMERAAEPAVTLSVPLGVDVGT
                     GPNWGAAH"
     gene            16609..17454
                     /locus_tag="VF589_00535"
     CDS             16609..17454
                     /locus_tag="VF589_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256088.1"
                     /translation="MNRRNFIAASLALALLGCAPSAGAAAPPFQPKRFSVVVRGAGPD
                     VILVPGLTASRDIWNGTVAAVPGYRYHLVQVNGFAGTAPGANASGPVVGALADEIAAY
                     AKARGLAAPAIVGHSMGGTLAMMVAARHPGVVGKAMVVDMLPQPAGLLGSSASGVRGL
                     AETLRGLTASPGGRELVSSLIGMFGNPQAEATRSDPDLVARVTHELALTDLTPELPRM
                     KAPLTVVYAVPDAESRAIVENGYKEAYAAKRDARLVRIDNSGHMVMCDRPTAFHAALR
                     AFLAR"
BASE COUNT         2639 a         6325 c         5936 g         2618 t
ORIGIN      
        1 atttcgggat cgatgacgag ccgctgcggc tgctccgccg cgagcgtggc gccgcccgcg
       61 aacggatccg gctgcggctg cggccggacc tgggccgccg cccgggctgg cggggcggcc
      121 ggctggaggc cgagaaagcc ggcggcggcg ctccaattgg cgagcagcca ggccatggcc
      181 gcgatgccgc cgatgaaggc gagcagcgga aggaacagca aagacagcag cgaccggcgc
      241 cggacggcgg ggctgctcac ctcttgatcc attctcatgc gccggattcc gtagcctcga
      301 tggcgtcacc tttgcacagc ttcgcggcaa cctccagcaa tgcatggtcg cgcgggacgg
      361 gagcgatcgc caccgccgac cacctccggc cagccgccgc cgccgccgcc gggctgatcg
      421 ccgccaccgc gatccgcgcg ggatcgagac cggccgcttc gaccaggccc ctgaacaggg
      481 cgcccgagcg cggcgaatgg atgagcgcca cggcgcccgc gcggagcgcc gcggccgcat
      541 ccggagggag cgtctcagac gcttcggcgg catagacgaa ccgccgcacc agccgcagcc
      601 gcggccgctc gagatcgaga tgatcgcggc cgcagagatg caggacgcgt tcgatcccgt
      661 ccgccgccaa ccgatcgagc agccttttac cgtcgccgcg gccgatcctg acgtcgccga
      721 agcccgcggc ccgcgccgcc gccgcgctgg cctcgccgac ggcatagcag gggtgggcca
      781 gaaacggcga caggctggcc cccgcatggc ttgccgcgtt ggcgctggtc agcatcaccg
      841 cctcgaccgc cgccggatcg ggtgcctgcc aggcgagcgg caccacctcg aacaacggcg
      901 cgaccaccgg atctacgccg agctcggcta tgcgccgcgc ggtgcgctcc gctcccgcct
      961 gcggccgcag gatcagcagc ttgcgcttca cccggcgaac agcgcccgca gcgacgggct
     1021 ggcgcggccg agcaagtgct gggcgagctc gagcggcgct tcgacgtcgc cggcggcaaa
     1081 gcgcgtctcg ccctgcacga cctcgccgcc atcatgggtg agcagctccg cccggaggtg
     1141 gatgatgtcg ccttcgctta gcgccagcgc cgccaccggc gagcggcagt cgccgccgag
     1201 cccttcgagc agccgccgct cggccgacac gcattggtgg gtctcggcgt ggctgatcgc
     1261 ggcgaccagc gcgcgcacct tcttgttctc ggcgcgaacc tcgaccccga tcgcgccctg
     1321 cgcgggcgcc ggcagcagat cgtcgagcgg ggtgccgatc tcccccagtc cgagccggtc
     1381 gagcccggcc gccgccagca gggtcgcgtc cgcctcgccc agctccagcc gccgcacccg
     1441 ggtcgcgaca ttgccgcgga aggcgacgat gttgaggtcg ggccgccgcg ccagcagctg
     1501 cgcggtccgc cgcggcgagg atgtgccgac ccgcgcccat cccggcagcg cttcgaggct
     1561 gacggcgccg agcagccggt cgcgggcgtc ggcgcgcggc agcatcgcgg cgatgcagaa
     1621 ttcgtcgggg cggaaggtct cgacatcctt catcgagtgg accgagaggt cggtgcgccc
     1681 gtcgatcagg tgggcgtcga gctccttggt ccacaggctc ttgccgccga cttccgacag
     1741 cggacggtcc tgtatcatgt cgccgctggt ggtgatcgcg acgatctcca tccgctccgg
     1801 accccagcca tgcgcctcgc gcaaggcggc cgccaccagc cgcgcctggg cgagggcaag
     1861 cggggagccg cgggtgccga tgcggagggg cgtgtccatg cggcgcttgt gctaacggct
     1921 aaggcaatgg cagggaagcc aggatgagcc tgatcctcgg catcgaatcg agctgcgacg
     1981 agaccgcggc ggctttggtc acttcggacc ggcaggtgct cgcccaccgg ctcgccggcc
     2041 aggagagcgc ccaccgcccc tatggcggcg tcgttcccga aatcgccgcc cgcgcccatg
     2101 tcgagatatt gccctcgctg atcgaggatg tgctgcgcga ggccgatctc tccttggacg
     2161 aagtggacgc gatcgccgcc acggcggggc ccggcctgat cggcggcgtc atggtgggcc
     2221 tggtcaccgc caagggcctc gcgctcgccg cggacaagcc gctgatcgcg ctcaaccacc
     2281 tcgaaggcca cgcgctgtcg ccgatgctga gcgcgccgga cctcgacttt ccctatctgc
     2341 tgctgctcgt ctccggcggc cattgccagt tactgttcgt cgaaggggtc ggccgttacc
     2401 gccgcgtcgc caccaccatc gacgacgccg ccggcgaggc tttcgacaag agcgccaagc
     2461 tgctcgggct cggctttccg ggcggcccgg cgatcgagcg cgcggccgcc gacggcaacc
     2521 cgaaggcggt gcgcttgccg cgcccgctgc tgggctcgga cgagccgcat ttctcctttg
     2581 ccggcctcaa gagcgcggtg ctgcgggcgc gcgacgccgg cctctacagc gccgaagaca
     2641 tagccgcctc cttccagcag gcggtcgtcg actgcctggt cgaccgcacc gcccgcgcgc
     2701 tcaacgaggc gcccggcgcc accgccctgg tcgtggcggg cggggttgcc gccaatcaga
     2761 gcgtgcgggc ggcgctcact tcattggcct cggttcacgg cctgccgttc gcggcgccgc
     2821 ctttgtggct gtgcaccgac aatgccgcga tgatcgcctg ggccggcgcg ctgcgctttg
     2881 ccgaggggct ggtcgacggc ctcgacgctc cggcgcgggc gcgctggccg ctcgatcctg
     2941 acgccgagcg ggcgcgcggc gccggggtca aggcatgaat atgcgcctcg gcgtggtcgg
     3001 cggcggcgcc tggggcacgg cgctggcgca ggtcgcggcg tcgggcggcg aggagaccct
     3061 gctctgggcg cgcgagcccg aagtcgtcca ttcgatcaac agccgccacg agaataagag
     3121 cttccttgcc ggcatcccgc tcaatgcggc gatccatgcg accggcgagc tggccgacct
     3181 caaggcctgc gacgcgctgc tggtcgtcac ccccgcccag catacgcgca aggtgctgtc
     3241 ggcgatggcg cgctggacca agccgctgat cctctgcgcc aagggcatgg aggaggcgac
     3301 caccaagctg atgcacgacg tcgcgcgcga ggagcatccc ggctgcgcca tcgcggtgct
     3361 gtcggggccg accttcgcgc atgaggtggc ggccggcctg cctgcggcgg tgacgctggc
     3421 ggtggaggat cgcggcgtcg gcgaacggct gcgcgcgcgg ctcgctctgc cctggttccg
     3481 gccctatttg tcggacgaca tcgtcggcgc ggagattggc ggggcggtca agaacgtgct
     3541 cgccatcgcc tgcggcgtgg tcgaggggcg cgggctgggg cagaatgccc gctccgcctt
     3601 gatctcgcgc gggtttgccg aaatgacgcg tttcgggctg gcgcgcggcg gccgggcggc
     3661 gacgctggcg gggctctccg ggctcggcga cctcgtcctc acctgctcct cggccagctc
     3721 gcgcaatttc agcctcggca aggggctcgg tgaaggctgg gacccggccg agctgctcgc
     3781 cgaccgccat acggtggccg aaggggccta taccgcgccg gtgctgaagc gggcggccga
     3841 ggccgccggc atcgacatgc cgatcgtcgc cgccgtctgc gccttgctca gcggggaggc
     3901 cgccgtcgac gcggtggtgg aaaggctgtt gtcgcggccg ctgaggagcg agggggcctg
     3961 aggccggctc actgggtgac gccgggtcag aagtcccagc aaatgccctt attctcccag
     4021 tcgccgtagc gggtcggctc gcggcccttg ggtccgccat gctcctcggc ggccgcatcg
     4081 ggctcagccg ggtccggcgc cggcaccggc cggctcttgg acaggtaagc gggcggcttc
     4141 acatggtctg ggcggcggcc ggccatcatc gtctcccgat tgaagagggg catgcgtctt
     4201 cccacatttc gggttgagga gaccggatcg caagatgaac catgtgaaga ccctgatgct
     4261 gctggcggcg ctgaccgcct tgttcatggc gctcggctac acgctgggcg gctcgggcgg
     4321 cgcattgatg gcgctgctgt tcgccgccgg catgaacctg ttcaccttct ggaatgccga
     4381 caagatcgtg ctccggatgc acaaggcgcg cgaggtggac gcccgctcct cgcccgaatt
     4441 gttcggcatc gtccgcgagc tggcggcccg cgccgggctg cccatgccga aggtctatat
     4501 cgtcgattcg ccgcatccca acgccttcgc caccggccgc aacccgaaga atgcggcggt
     4561 cgccgccacc acgggcctgc tcgccctctt gagccgcgac gaagtggcgg gggtgatggc
     4621 gcacgagctc gcccatatca ggaaccgcga cacgctgatt atgacgatga ccgcgaccat
     4681 tgccggcgcg atctcattcc tggccaattt cgggctgttc ttccgcggcg gcgacgacaa
     4741 tcgcggcaac atgctggcga tgatcgcggc ggtgatcgtc gcccccttcg ccgccatgat
     4801 cgtccagctc gcgatcagcc gcacccgcga atatggcgcc gatcgcggcg cggccgagat
     4861 cagcggcaac ccgcgcgcgc tcgcctcggc gctcgccaag ctccagcagg gcgcggcccg
     4921 cgtccccaac ccggtcgcga tgcgcaaccc ggccgccgcc tcgctctaca tcgtgccgtc
     4981 ggcgaccggc cgcgacagca tgttctcgac ccatcccgac accggcaacc gcatcgccgc
     5041 gctccacgcc atggccgacg agctcggcct cgcctcgccg ccgccggcgc ttggccgcgg
     5101 cgccccgccg cgcagctcgg tgccgaagac gcggcgctcc gccagcgcgc tcgatccgct
     5161 ggggcgcggg gactgagccg ggaagatccg atcggcacgg cgtcgcgccg ggccgctttg
     5221 cgcctgctcg acgcggtgct gcgcaagggc ctgccgctcg aagcggcgct cgacaccgcc
     5281 gcgcgcgacc tcgaccgggc cgacgatcgc ggcttcgccc atgccatagc cgccgaggcg
     5341 ctgcgccgcc tcaccgatct cgatgcattg atcgacagcg cgaccaagaa gccgctgccc
     5401 gatgacgcca aggcgcgctt cgcgctccgg atcgcgctgg tccaggcgct ggcgatggag
     5461 acgccgcacc atgccgcgat ctcgacggtg ctgccgctgg tcgagggcgg cccgcgcaag
     5521 ctggtgcacg gcgtcttcag cgcgctgatg cgcagcggcg tcacgcttcc cgaggtgccg
     5581 agcctgtcgc cgcaggtcga ggcgcgctgg gagacggcct ggggcagcgc ggtcgcccgg
     5641 gcggcggcgc gggcgatggc ggtgccgccg ccgatcgacc tggtgctcgc cggggccgaa
     5701 gcgcccgagg ggctcaaggg cgaaagcttc atgcccggtc acctccgcct cgcccggggc
     5761 acggcgattg cggggctgac cggctatggc agcggcgact ggtgggttca ggacgtctcc
     5821 gcctcgatgc cggcgcggct gctcggcgat gggcgggggc gcagcgcgct cgacctctgc
     5881 gccgcgcccg gcggcaagac gatgcagctt gcctcggccg gctttgatgt caccgccgtc
     5941 gatgcgtcgg aaagtcgtct ggcgcgactt cgcgagaatc tggagcgaac ccatctctcc
     6001 gcgcagatcg aggccgccga tgtgatgaat tggcagccgg ccgacccggt cgacgccatc
     6061 ctgctcgacg cgccctgttc cgcgaccggc atcttccgcc gccaccccga tgtcctccac
     6121 cgcgtccgcc cgcgcgtcat cgccgagctg gcggcgatcc agcaggcgat gctcgcgcgc
     6181 gccgccggct ggctgaaacc gggcggcacc ttggtctatt cggtctgctc gctggagccg
     6241 gaagagggcg agaagatcgt cgaggccttc ctcggcgccc gccccgatta tgcgctcgac
     6301 cccgtcacgt ccgacgagtt gccgccaccg atgcgcccca atgacggcat tttgcgcatc
     6361 ctcccgggcc tctacgagga gcagggcggc gccgacggct tctttatcgc gcgtttcagg
     6421 cgcattagcg ggggataagt cgcccgcttc cttgccccgc gactcgggcg cggttactga
     6481 ttcgctgtgc agcagccggt ccgcatcgcc ccctcgatcc tctcggccga tttcgcgcgc
     6541 ctgggcgagg aagtgcgggc gatcgacgcg gccggcgccg actggatcca tgtcgatgtc
     6601 atggacggcc atttcgtccc caatctgacg attggcccag gcgtcgtgaa agcgctccgc
     6661 ccgcataccg ccaagacgtt cgacgtccac ctgatgatct cgccggtcga caatttcctc
     6721 gccgccttcg ccgaggccgg cgccgacatc atcaccgtcc atcccgaggc gggcccacac
     6781 cttcaccgca cacttcagca catcaagttg ctcggcaaga aggccggcgt ctcgctcaac
     6841 cccgccactc cggcgaagat gctcgattat gtgctggagg aggtcgatct cgtgctggtg
     6901 atgagcgtca accccggctt cggcgggcag agcttcatcg acagccaact ccgcaaggtg
     6961 gaggcggtcc gcaagcagat cgacaagacc ggcttgtcca tagacctcga agtcgacggc
     7021 ggcatcgacc cggatacggc ccgccgcgcc atcgaggccg gagccgacgt cttggtcgcc
     7081 ggcaccgcca ccttccgcgg cggcgccgat cattatgccg acaatatcag gaggcttcgc
     7141 ggatcatgag cctgctcgag cgcatccacg aggccgatgg gcagcaggag atcgagcccg
     7201 gcaagaggct gatccgggtc ggtgacgacc gcggcgtctc gctcgccgag cggctcgcct
     7261 accggctcca ccggctcgcc tggcgcaccc ccttccacag cttccggctg cgcgggcgct
     7321 tcccgctgaa gctgctgacc gtgcccaagg acccgatcgc gggcgacaag gccgcgggcg
     7381 aggcgatcct cgcgggcaat atcgttcacc gcggcgccag ccttccgatc gacaggctcg
     7441 atttcaccga tccgtccatt cccgccgaat gcaccgaata tctgcagagc ttcgcctggc
     7501 tgcgcgacct cgccgccgcc gcgacccgcg aaaagggcgc gcgggtggcg gagaagatct
     7561 gccagaaatg gctgagcgct tattccgcca gcgtgtcgga gccggcctgg cgcgccgatc
     7621 tgtggggacg ccgcatcctg ttctggacgg cttacgcgcc ctacatcctg tcgagccgcg
     7681 acatggtcta ccgttcggcc gtgctccaca ccctcgcccg cggcgcccgc cacctcgatc
     7741 gcggcgccga caaggccacc gcaggcctgc cgcggatcgc cgcctggtgc ggggtgatcg
     7801 cgtcggcgat cgtcgtgcag ggcggccccg cccggctcgg caccggcgag ggcgggctgc
     7861 gccgcgcgct cgacgcctcg ctccacgagg atggcggcct catcagccgc tcgccgctcg
     7921 agcaactggc cttggtcgag ctgctcagcc agcttcgcgc cgtctatatg tcggcccgcc
     7981 gccagatgcc cgaagacctg gccgaggcgc tctccggctc cgtcgccgcg ctgctcgcgg
     8041 tgacgctcgg cgacgaggcg ctgtcgagct ggcagggcgg caatatgggc tcgaagcggc
     8101 ggctcgccgc ggcgatcgag ggcagcggcg tcacggcccg gccgctccgc caggcgcgcg
     8161 gctggggcta ccagcggctc gccgcgaaaa agagcgtggc ggtgttcgac gccgctccgc
     8221 cgcccgccag ccgcgcgctc agcggcggct gcgcctcgac cctggccttc gagttcagcg
     8281 acggcgccga gcggctggtt gtcaattgcg gcggggtcgg cccgcggatc tcggcgctcc
     8341 cgaccgacct gatccgcggc ctgcgcacca ccgccgccca ttccaccctg attctcggcg
     8401 accgcaactc gaccgcgatc cacgaggacg gcacgctcgg caagggggtg gggcaggtcg
     8461 agctgtcgcg cgacgagacc gccggcacca ccatcgtcga ggcgagccac gacggctatg
     8521 tccgccgctt cggcctcgtc caccagcgcc agctcaccct cacctccgac gggctcgagc
     8581 tgcgcggcga ggaccggctg ctgcccgagg gccgccgccg ccgctcggag ccgaccgcct
     8641 tcgccgtccg cttccacctc gctcccgccc tcgaagtgac cagcaccgcc gacggccagg
     8701 gcgctttgct ccgcatccgc ggcggcgccg tctggcaatt ccgctgccgc ggcggcgcgc
     8761 tggcgatcga ggacagcctc tcggtcgacg gcgaggcgcg gccccacagc tcgctccagc
     8821 tcgtcattac cggcgagacc ccgcccgccg gcatgagcat cagctggctc ctgaagcgcg
     8881 cggggtagcg gcgggcgccg gacgggtcac gccgagtcga gccggcctcg tcgacacaag
     8941 tcgtcaccct caacttgttt cagagcctgc cccgcacccg atccgggggt ccattttgcc
     9001 gccccgcacc gagcccgcaa ttgaatgctg aaacaagttc agcatgacgc taggagccga
     9061 accttcctca ttgaccagga ccatccatgt cagacgtgaa gatcacccgc gcgctcctgt
     9121 ccgtttccga caaagcgggg ctcgccgaac tgggccaggc gcttgccggg catggcgtcg
     9181 agctggtctc gaccggcggc accgcgcggg cgctgcggga ggcgggcctg acggtgcgcg
     9241 acatctccga tctcaccggc tttccggaga tgatggacgg ccgcgtcaag accctccacc
     9301 ccaaggtcca tggcggcctg ctcggcgtcc gtgacgaccc ggcgcacgcg gcggcgatgg
     9361 cggagcatgg catcggcgcg atcgacttcg tcgtcgtcaa cctctatcca ttcgccgcga
     9421 ccgtcgccaa gggcgcgggg cgggacgaga tcgtcgagaa tatcgatatt ggcggcccct
     9481 ccatggtccg ctcggccgcc aagaaccacg ctttcgtcac catcgtcacc gatcccgcgg
     9541 attatgccga gctgatcgag acgctcgccg ccggcggagg catgacgtcg ctcgacctca
     9601 ggaagcgcct cgccgccaag gccttcgcgg ccaccgcggc ctatgacgcg gcgattgcct
     9661 cctggttcgc cttcgccgac cagggccagc tcttccccga cctgctgcca ctggcgatgc
     9721 acaagcgcga agagctgcgc tacggcgaga acccgcacca gcaggcggcg ctctatcttc
     9781 cggccgggcc gcatgcgcgc ggcatcgccc aggcccgcca ggtgcagggc aaggcgctca
     9841 gctacaataa ttacaacgac gccgacgcgg cgctcgagct ggtcgccgag ctgcgcggcg
     9901 gcgatccggc ttgcgtgatc gtcaagcatg ccaatccctg cggcgtcgcc gccgcgggca
     9961 ccctcctcca ggcctatcag caagcgttcg actgcgaccc cgtctcggcc ttcggcggca
    10021 tcatcgccgt caaccgcccg ctcacccgcg acgccgccga ggcgatgacc cggatcttca
    10081 ccgaggtcgt gatcgcgccc gacgccaacg aggaagcgcg ggcgatcttc gcggccaaga
    10141 agaacctccg cctgctgctg accggcgacc ttcccgatcc ggcgcggccg gggctggcgc
    10201 tgaagtcgat cgcgggcggc tatctcctcc agtcgcgcga caacggccgg gtggagcgcg
    10261 gcgacctcac ggttgtcacc aagcgcgtgc ctacggacca ggagctcgcc gactgcctgt
    10321 tcgcctggac ggtcgccaag cacgtaaagt cgaacgccat cgtctatgcg aaagacggcg
    10381 ccaccgccgg catcggcgcc ggccagatga accgactcga atccgcccgc atcgccgcct
    10441 ggaaggccaa ggacgccgcc gagaaggcag gctggcccga gccgcgcacg atcggctcgg
    10501 cggtcgcctc ggacgctttc ttccccttcg ccgacgggct gctcgccgcg gtcgaggccg
    10561 gcgccaccgc cgtcatccag ccgggcggat cgatccgcga cgacgaagtc atcgccgccg
    10621 ccgacgaagc ggggctggcg atggtgttca ccggcatgcg ccacttccgc cactaggtcg
    10681 ggctggaacc catcgcgggc agcgcccgtt gctcccccgt cagcaaggga gacccacatg
    10741 aacgtcggcg acgagcgcag tttttcctac tggatggaat tcgcccccgt gctcgacgcg
    10801 ccgccgctga cctccgacgc gacctgcgac gtggtcgtga tcggctccgg catcgccggc
    10861 ctgtcgaccg cctatgagct ggcccgcttc ggccgctcgg tgatcgtcat cgatcgcggc
    10921 aagatcggct ggggcatgac ggcgcgcacc accgcccacc tcgccaccga gctcgacgat
    10981 ttcttttccg agctgatccg cgtgcgcggc gaagacgatg cgcggctgtg gcacgacagc
    11041 caggtgacgg cggtcaaccg gatcgaggcg atctgccgcg acgaaggcat cgatgccgat
    11101 ttcgcccgcc tcgacggtta tctgttcccg gccgaggaag cgcaccgcgc cgagctcgag
    11161 gaggaatatc aggcttgcaa ggcgatcaac gtcaacgtcg aatgggtcga ccgcgcgccc
    11221 gtccccggca aggataccgg gccggcgctg cgcttcccca accagggccg cttccatccc
    11281 accaaatatc tcgccggcct cgccggcgcg atcgtcgccc gcggcggtcg gatctacgcc
    11341 gacaccgccc atgcctccga ccaggaaacc gacgacggcg tcgagatcac caccgaagcc
    11401 gggaccgtca tccgcgcctc ggcggcggtg ttctgcacca actcgccgac caacgacaaa
    11461 gtcgccatcc acgccaagca gacgccggac cgcacctatg tggtggcggg gcgcgtgccg
    11521 aagggctcgg tacccgacgc gctcgtctgg gacacgtacg aagcctatca ctatgtccgc
    11581 atccagccgc tgagcgaaac cgaggatctg atcatcgtcg gcggcggcga ccaccgcacc
    11641 ggcgaggcgg acgacatgga caagcgcctc gacgatctcg aaagctggac ccgcgaacat
    11701 tatccgagct tcacccgcgc cgattatcgc tggtcgggcc aggtgctcga gccgatggac
    11761 ttcatgccct tctccggccg caacccgggc aacaagaaca tctacatcca caccggcgat
    11821 tccggccagg gcatcaccaa cggcgtcgcc ggcagcctca ccatcatgcc gctcatcatc
    11881 ggcgaggaca gccgctacgc gccggtgctc gatccgggcc gcaagtcgtt cacctcgacc
    11941 tcctcgatcg gcgagttcgt ccgcgagcag gccggctccg ccaagaatta catggagcat
    12001 atcaccggcg gcgagatcgc ttcggccgac gagctcgcgc cgggcgaggg cgggctgatg
    12061 cgcaaggggc tgcacaagat cgccgtctat cgcggcgagg acggttcggt cacccagcgc
    12121 tcggccgtct gcacccatct cgggtgcatc gtccactgga acaatttcga gaaatgctgg
    12181 gattgcccgt gccacggctc gcaattcgcg cccgacgggc aggtgctcaa cggcccggcg
    12241 gcaaagcccc ttgcggaggt ggaagagtag cttcctcgcc gcgcttcatc cggaacagca
    12301 cgcggtcctc atgggtgaag ccgcgcgtcc acagcaccgc cccgaaggtg acgaggatga
    12361 ccggaatccc gaccaccagc tcgacgaatt cgagcgagcg cgggagcaag gtgaagccgt
    12421 agccgaccac cgtcgccgcc gccgccgccc agatcagcgg ccagcgccag ccctgcaccg
    12481 gcgcgccgag gatgcggcag agcagccggg ccttgagcac cgacgacatg ccgagcgcca
    12541 gcatcagcgc gatcgccggc cccgccgcct ggaaggtcgc cggccagccg agcgcgcgca
    12601 tgccgagaat gagcgcgacc gacagcccgg cctgcaccgc tatcgagaag accgagatca
    12661 tcagattgcg gtgccgcgcg acatagacca atgccgattc cgagaccgcg gcggtggcgg
    12721 cgaccacttc ggcggcgagc aggaaggcca gcgcgccggt gccgccgatg aaggcggcgc
    12781 cgaccaggcc catcacggct tcggcgggga tgccgagcgc cagcgcgatc gcagcctggg
    12841 cggcgatgat ccagaagccg acctgccgca cctgccgcgc cactgcggcc ttgtcgcctt
    12901 cggccagctt gtgggtgatg accggagaca ggatcgggtc gaagctggtc ttcagcttct
    12961 ggggcaatga cgccacttgc tgcgcgacat aatagacgcc gaccacggcc ggcgagaaga
    13021 acaggccgag gatggcgatg tcgatccggc gcgacgccca ttcgatcgcg tcggccgcgg
    13081 ccagcggcgt gttgcgccgc gccagcgccc acaaggtcac cgggtcgggc cgccagccag
    13141 tcggcgcgcc ataggtccgg aacaagggcc acaaagcggc gataaacgcc gccagcatcg
    13201 acagcacata agcgatgatg aggccgtcgc gggacgagag gtaggagagc aggaacgccg
    13261 cgatgctgat cgtccacggc tcgacgatcg cccgcgacgt caccgaggcg cggacattgt
    13321 ggcgataggc gagcgcggcc aaggccacgt cggcgccggc gatggcgaac acgatcagcg
    13381 gcagcagcca ttcgagcccg acgatcgggc tgttcgggaa catcgcctgc gggaagatgg
    13441 ccagcaggat ggtcgcgacc gccgaggcga ggaaggcgac cagcatcgcg tcccagacga
    13501 tgcagacatg cggcttgtcg gtcgacgaaa gctgctgcgc gagcccccgc ttcaacccca
    13561 gggtcgcgat ctgggccgcg aactcgacga tcaatatcgc ataagcgaac cgcccgagcg
    13621 cctcggcgcc atagatgcgg cccgcgatga acaggaaggg cagccgcgcc gccagccgca
    13681 gcaggaatcc gaagaaattg gtccgcccgc ccctggcgag cgccgcgatg tcgtcgcccg
    13741 ccggtgcggg ctgggtcaat gcgccgcgcc ccagttcggg ccggtgccga cgtcgacgcc
    13801 gagcgggacc gagagcgtca ccgccggctc ggcggcccgc tccatcacgc ggcggatcac
    13861 cgcgccggcg cgctcgacgt ccgcctcggg cacttcgaac accagttcgt catggacctg
    13921 catcagcatc cgggtggagc caagcccctc cgcctccagc gctgggcaca tgcggaccat
    13981 ggcgcgcttg atgatgtcgg cgctggtgcc ctggatcggc gcgttgaccg cggcccgctc
    14041 cgccccctgc cgctcgtgct ggaccttgct cttgaggccg gggaagtggg tcttgcggcc
    14101 gaacaaggtg gtggtgaagc cggtctcgcg cgcgccggtg agggtctcgt ggatgtagtc
    14161 gcggatgccg gggaagcggt cgaaatagcg gtcgatgatc gcctgcgcct cgtccgcggt
    14221 gacgccaagc cgtcccgcga ggccccagcg cgagataccg tagaggatcg cgaaattgat
    14281 cgtcttggcg ctcgcccttg tatcgcgatt ggcctccccg aatagctcct gcgcggtcat
    14341 gttgtggatg tcggcgccgg ccgcgaaggc gtcgcggagc tgcggcacgt cggccatgtg
    14401 ggcggccagc cgaagctcga tctggctata gtccgcggcc agcacgacat ggccgggctc
    14461 ggccacgaag gcgtcgcgga tgcggcggcc gatctcggtt cggatcggga tattctgcag
    14521 gttcgggtcg gtcgaggaca gccgcccggt ctgcgcgccc gacaggctgt agctggtatg
    14581 gacgcgcccg gtctccttgt tgatctgctg ctggagcgcg tccgtatagg tcgacttcaa
    14641 tttggtgagc tgccgccatt cgagcaccag ccgcgcgatc ggcacgcctt cgcgcgacag
    14701 ccgctcgagc tcgttgacgt cggtggacca ggtcccggat ttaccttttc ggccgccctt
    14761 cagctgcatc tggttgaaca ggacgtcgcc cagctgctgc gggctgccga tggtgaactt
    14821 gcaccccgcc tcgccgcaga tccgctcttc cagctccaat atctgctggt tgaactcggt
    14881 cgacagcttg tggagctcgt cgcggtcgac cttgacgcct tcgcgctcca tccgcgccac
    14941 caccggtacc agcggccggt cgaccatctc gtagatgcgc gtcacccgct cgtgcggcag
    15001 ccgcgtcttg aaccgcttcc acagccgcag cgtgacgtca gcatcctcgg ccgcatattc
    15061 ggtcgcccgg ccgagcggca cctggctgaa cttgatctgc ttctgcccgg tcccgcacaa
    15121 ttccttatag gtcaggcact gatggtcgag atgccgggcg gcgaggtcgt ccatggcgtg
    15181 gctggtgagc ccgcccgcat cgaggttgaa gctcatcacc aggctgtcgt cgatcggcgc
    15241 gaccttgatc ccgcgccggt cgaacacgat ccagtcgaac ttgaaattgt ggccgacctt
    15301 caggaccgcc ggatcctcga gcatgggctt gagcttcgac agcgccagct cggccggcac
    15361 ctgctcgggc cgctccgaca gcaaatcgtg gccgccatgt tcgagcggta tgtagcaggc
    15421 cttgccgcat tcggtggcga ggctgacgcc gaccagcttg gcggtcacgc aatcatagcc
    15481 gtcggtctcg ctatcgaccg cgaccagtcc gttcgcgctc gcttccgcga tccagcggtc
    15541 gagcgccgcc gcgtccgtca ccgtctcata ggcggagcga tcgatcttcg gctccggccg
    15601 cacctctcgt tgcaccggcg ccgccgaggg cgagtccgac ggcgcccgca cctgcgaggt
    15661 gccgttcaat cgcgccagca aagatcggaa gccctgatcc tccaggaact cgcgcagcgg
    15721 cccgtccgga atgcccttca tctggagcgt gtcgagcggc tcgggcagcg gcgaatcgca
    15781 caccagccgc accagctcgc gcgacatccg cgcggccgcg gcatgttcgg taaggttctg
    15841 cttcagcttg ggcttggcga tctcgtcgac atgggcgagc aaagtctcga cgtcgccata
    15901 ttgcttgatc agctgggccg cggtcttcgg cccgaccccc ggaacgcccg gcacgttgtc
    15961 gacgctgtcg cccatcagcg ccagcacctc gcccaattgc tcgggcggca cgccgaattt
    16021 gtcgatcaca tagtcgcggc cgatgcggcg gtcgttcatg gtgtcgagca tgtcgaggcc
    16081 gggctcgatc agctgcatca ggtccttgtc cgatgagacg atggtgacct tccacccctc
    16141 cttcagcgcc gacttcgaat agcaggcgat gatgtcgtcg gcctccagcc cgtcctcttc
    16201 gatgcagggc agcgagaagg cgcgggtggc gtcgcggatc agcgggaatt gcgggacgag
    16261 gtcggggggc ggcggcggcc ggttggcctt gtattcgcta tacatgtcgt tgcggaaggt
    16321 cttggccgag gcatcgaaga tcaccgccag atgggtcggc ccgtcctcct tgtggaggct
    16381 ttcggccagc ttccagagca tcgtcgtata gccgtagacc gccccgacat tgaggccgtg
    16441 ccggttggtg agcggcggca gccggtgata ggcccggaag atgtagctcg atccgtcgac
    16501 gaggtaaaga tggggggagg acattgccga gggtctagcc tagtcgctcc catatgggga
    16561 agccggaagg tcatgcgagg ttcaagcggc cgcgctagaa gcgccgccat gaaccgcaga
    16621 aatttcatcg ccgcctcgct cgctctggcc ctgctcggct gcgcgccgtc ggcgggcgcc
    16681 gccgccccgc cgttccagcc gaagcgcttc tcggtggtgg taaggggcgc cggccccgac
    16741 gtcatcctcg tccccggcct cacagcgtcg cgcgacatct ggaacggcac cgtcgccgcc
    16801 gtccccggct atcgctacca tctcgtccag gtgaacggct ttgccggcac cgcccccggc
    16861 gccaacgcta gcggcccggt ggtgggggcg ctcgccgacg agatcgccgc ctatgccaaa
    16921 gcgcgcgggc tggccgcgcc ggccattgtc ggccattcga tgggcgggac gctggcgatg
    16981 atggtcgccg cgcggcatcc cggggtggtc ggcaaagcga tggtggtcga catgctgccg
    17041 cagccggccg gcctgctcgg ctcctcggcc agcggcgttc gcggccttgc cgagaccttg
    17101 cgcggactca ccgccagccc cggcgggcgc gagctggtca gctcgctgat cggcatgttc
    17161 ggcaatcccc aggccgaggc cacccgcagc gaccccgatc tggtcgcccg cgtcacccac
    17221 gagctcgccc tcaccgacct cacccccgag ctgccgcgga tgaaggcgcc gctcaccgtg
    17281 gtctatgcgg ttcccgacgc ggaaagccgc gcaatcgtcg agaatggcta caaagaggct
    17341 tatgccgcca agcgcgacgc caggctcgtc cgaatcgaca atagcgggca catggtgatg
    17401 tgcgaccgcc ccaccgcctt ccacgccgca ctgagggcgt ttctggccag gtgatcctcc
    17461 gccccggcag gagcgatcac ggcatcagca cggtatccac ggcctcgggc agcagctc
//
LOCUS       DASVRK010000012         9045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_126925, whole genome shotgun sequence.
ACCESSION   DASVRK010000012 DASVRK010000000
VERSION     DASVRK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126925"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..514
                     /locus_tag="VF589_00540"
     CDS             8..514
                     /locus_tag="VF589_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX8256089.1"
                     /translation="MLLLYLLGYALFAVMLPRPAPERPTDAIVVLTGGAKRIERGIDL
                     LSRGRADRMLMSGVERTVRPVELALRYQADERLFECCIDLGRESVDTRSNAEEVSRWV
                     ERRKVKTLRLVTTDWHMPRARFELSRQLGDDVVMISDAVTSNPSFRTLFTEYNKYLLR
                     QAAVVVGI"
     gene            514..1200
                     /locus_tag="VF589_00545"
     CDS             514..1200
                     /locus_tag="VF589_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8256090.1"
                     /translation="MNLIRSMLFSLFFYPGSALFVALAFPAALLGREAVIRVTYGWAR
                     YHHWCARLLLGIKVRVEGTAPRGATLVAAKHQSMFETIELLRYLDRPAVVLKRELADL
                     PGWGWVAQTYGCIPVDRDGGAAALRRMMKAAKTAIAEGRPILIFPEGTRVAVGEQPPL
                     QSGFAGLYRALGLTVVPLAVDSGRLWPRRSFVKRPGVVTFRFGESLAPGRPRGEVEAA
                     VHKAINALEA"
     gene            complement(1197..2252)
                     /locus_tag="VF589_00550"
     CDS             complement(1197..2252)
                     /locus_tag="VF589_00550"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEX8256091.1"
                     /translation="MPGSATEGGEATVRAAFATQAGWCATMGSPFTAKLCTLLGERLD
                     RSTEVGRRTLDWPGNPDGWADGLPLRFCGGLHALVRSGAPLARCYPPNPTPDDETLWA
                     AIRRTLEDEGPALLAWLDHPPQTNEVGRAAALMSGLLVVAATWPLPIRLLELGASAGL
                     NLIPDRYRYTLGSAAAGDMSSPLHLQPEWSGASPPPADVRIQSRRGVDLRPLDIRRDA
                     ERLLAFVWPDQRQRLAQLETALAIAAADPPPVDAADAADWLEAELPPDPLPGATRVVM
                     HSVAYQYFPESSQRRIAARIAEAGAAARADAPLAWLRFEKDPGDHGTTLRLATYPGGG
                     DRLLAHCQPHGRTIDWC"
     gene            complement(2233..4608)
                     /gene="pheT"
                     /locus_tag="VF589_00555"
     CDS             complement(2233..4608)
                     /gene="pheT"
                     /locus_tag="VF589_00555"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX8256092.1"
                     /translation="MKFTLSWLKEHLDTSAALDEIVEALTGVGLEVEGVENPAEKLAP
                     FTIAKVLTAERHPQADKLQVLTVDTGAGEPVQVVCGAPNARAGMLGVFGAPGAYVPGS
                     DLTLKIASIRGVESRGMMCSVRELELGDEHDGIIALPDDAPIGASYPQWAGLDDPVID
                     VAITPNRQDAMGVHGIARDLAAAGLGTLKPIAVAEVPGRFPCPIEIRTDDPQGCPAFY
                     GRVVRGVKNGASPAWLQQRLKAVGQRPISALVDMTNYIMLSYGRPLHVYDLSKLEGAI
                     SARRARPGEQVLALNGKTYALDETMTVIADEAHVHDIGGIMGGEESGVTEATTDILIE
                     CAYFDPEHIARTGQKLGLTSDARTRFERGVDPAFVETGLQLATAFTIELAGGEPSEPV
                     RAGTPPIEARIIGYSPNRCAELGGLAVPEDRQQAALERLGFGVTVEETWEITVPSWRR
                     DVIGAADIVEEVIRIEGMDKVPSTPLERAPGVARPTATPEQLIERRARRAAAARGLHE
                     AVTWSFVAEAEAAPFGGSAWMLDNPISEEMKAMRPSLLPGLLAAARRNIDRGAAGVRL
                     FEIGRRYLADGERAALGLVLAGERGPRHWRTGKAQGFDAYDAKAEALAILAAAGAPVD
                     NLQVLGDASLVHHPGRSGRLCLGPKTALAEFGELHPSTLKAFGLDGPVVVAEIFLDLI
                     PQRRDAGHRRSAYAPPPLQAVKRDFAFLVDAETPAEALLRAVRGADKAAIVGVGLFDM
                     FTGPGVPEGQKSLAVEVTLQPGEKSFTDEELKAISARIVAAATKAGARLRD"
     gene            complement(4759..5838)
                     /gene="pheS"
                     /locus_tag="VF589_00560"
     CDS             complement(4759..5838)
                     /gene="pheS"
                     /locus_tag="VF589_00560"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX8256093.1"
                     /translation="MSDLELMSRELLGRIDAATDLPALEAERVAALGKQGVVTQLLKT
                     LGGMSPEQRQQGGPRIHALRERVADAIASRKAALEAAELDRRLATEMLDMTLPVPPGL
                     QGSVHPVSQVMDELAEIFADLGFSVATGPEIEDDWHNFTALNIPESHPARAMHDTFYF
                     DRSDGAGHRMLLRTHTSPVQVRAMQSQPPPIRIIAPGRTYRSDSDATHTPMFHQVEGL
                     VIDKGIHLGHLKWTLETFLKAFFERDDIVLRMRPSYFPFTEPSAEVDVGYTMVKGRRV
                     LGGTEGWMELLGSGMVHPRVLANCGLDPDEWQGFAFGCGIDRLAMLKYGMDDLRAFFD
                     GDLRWLKHYGFSALDVPTLSGGVGA"
     gene            complement(6067..6462)
                     /locus_tag="VF589_00565"
     CDS             complement(6067..6462)
                     /locus_tag="VF589_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX8256094.1"
                     /translation="MILVDSGVWIDFFRGTLSPHTDVLNGLLGRQAVAVADLILVEVL
                     QGFDRDSEFADALRLLSLPVFVRTGGREVALQAAANYRLLRARGITVRKTIDALIATY
                     CILHSTPLLFQDRDFDPFVEHLGLVSAMA"
     gene            complement(6462..6653)
                     /locus_tag="VF589_00570"
     CDS             complement(6462..6653)
                     /locus_tag="VF589_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX8256095.1"
                     /translation="MRTNIDIDDALLAEAMKLTGVRTKREAVEIALRGLVQLGRQGEL
                     RNWRGKLHWQGDLETMRLD"
     gene            complement(6692..8098)
                     /locus_tag="VF589_00575"
     CDS             complement(6692..8098)
                     /locus_tag="VF589_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX8256096.1"
                     /translation="MAADRKLYLGGRLRRLRRELGVSQSAMAGELGMSPSYLNHLERN
                     QRPVTAQVLLRLAQAYEVDLKSFTAEGEGTGTEDLAEIFADPMFGDLGIPRYELLEVA
                     DNAPSVADAISRLYTALVERRNAPPGAAPDGETSHVTPETWVRDYIQAQRNYFGYIEE
                     AAETLAGALGDRPGIFAALARRLKEGFGVEARVVPPELLDGTSQHYDYHRRRLMLSEL
                     LRPESRAFAVAYQVALFEFRPLLARMLESAAPPDMPTRRLLHMSFANYAAGAILMPYA
                     AFLGAAERHRYDIDRLCAEFGASAEQVAHRLTTLGRAGARGIPFFMLRVDAAGNISKR
                     FAGESFPFSRFGGTCPRWNLHAAFRTPERVITQIVETPDGQRFFTVSRAVERAVRLDP
                     RESSQLAIGLGCDVKFAPKLVYADGLDLAEPYVTPIGPACTICPRIRCPQRAAAPAGR
                     TLMVNEMQKTISPYPFGG"
     gene            8229..>9045
                     /gene="aceA"
                     /locus_tag="VF589_00580"
     CDS             8229..>9045
                     /gene="aceA"
                     /locus_tag="VF589_00580"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX8256097.1"
                     /translation="MSDFSSVVPAPADRFQGISRPYDVAEVERLRGSVPIEHTLAKRG
                     ANRLWELLKSEDYVHALGALSGNQAMQMVRAGLKAIYLSGWQVAADANTAGAMYPDQS
                     LYPANAGPELCRRINKTLQRADQIEHAEGGAQRDWFVPIVADAEAGFGGPLNCFEIMK
                     AYIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTQAHIRNLDAARLAADVCGVPTVLVA
                     RTDAESAKLITCDVDGRDHEFLTGERTPEGFFRLREGTGVDHCIRRGLAYAPHA"
BASE COUNT         1402 a         2941 c         3294 g         1408 t
ORIGIN      
        1 ctcggccgtg ctcctgctct acctcctcgg ctacgccttg ttcgcggtga tgctgccgag
       61 gccggcgccg gagcggccga ccgacgccat cgtcgtcctc accggcggcg ccaagcggat
      121 cgagcgcggc atcgacctcc tcagccgcgg ccgggccgat cggatgctga tgtcgggcgt
      181 cgagcgcacc gtccgcccgg tcgagctcgc cctccgctac caggccgacg agcgattgtt
      241 cgaatgctgc atcgacctcg gccgggaatc ggtcgacacc cgctccaatg ccgaagaggt
      301 gtcgcgctgg gtcgagcggc gcaaggtcaa gacgctgcgg ctggtgacca ccgactggca
      361 catgccgcgg gcccgtttcg agctgtcgcg ccagctcggc gacgatgtcg tcatgatctc
      421 cgacgccgtg acgtcgaacc cgagcttccg cacccttttc accgaatata acaaatatct
      481 cctccggcag gcggcggtgg tcgtcggcat ctgatgaacc tgatccgctc aatgctgttc
      541 tccttgttct tctatccggg gagcgccttg ttcgtcgcgc tcgccttccc cgcggccctg
      601 ctcgggcgcg aagcggtgat ccgggtgacc tacggctggg cgcgctacca tcattggtgc
      661 gcccgtctgc tgctggggat aaaggtgcgg gtggagggga cggcgccgcg gggggcgacg
      721 ctggtggcgg ccaagcacca gtcgatgttc gagacgatcg agctgctgcg ctacctcgac
      781 cggccggcgg tggtgctgaa gcgggagctg gcggacctgc ccggatgggg ctgggtcgcg
      841 caaacctatg gctgcatccc ggtcgaccgc gacggcggcg cggcggcgct ccgccggatg
      901 atgaaggcgg ccaagaccgc aatcgccgag gggcggccga tcctgatctt cccagagggt
      961 acgcgggtgg cggtgggcga gcagccgccg ctgcaatcgg gctttgccgg cctctatcgc
     1021 gcgctcggcc tcaccgtggt gccgctggcg gtcgacagcg gccggctgtg gccgcggcgc
     1081 agcttcgtca agcggccggg cgtcgtcacc ttccgcttcg gcgaaagctt ggccccgggc
     1141 cggccgcggg gtgaagttga ggccgccgtc cacaaggcga tcaacgcgct cgaagcctag
     1201 caccagtcga tcgtgcggcc gtgcggctgg caatgtgcca gcagccgatc gccgccgccg
     1261 ggataggtgg cgagacggag ggtggtgcca tgatcgcccg gatccttctc gaagcgcagc
     1321 caggcgagcg gggcgtcggc gcgggcggcc gccccggcct cggctatgcg ggcggcgatc
     1381 cgtcgctgcg agctttcggg gaaatattga taggcgacgg aatgcatcac cacccgcgtc
     1441 gcgcccggca gcggatcggg cggcagctcg gcttcgagcc agtcggcggc gtcggcagcg
     1501 tcgacgggcg gcggatcggc ggcggcgatg gcgagcgcgg tttcgagctg ggccagccgc
     1561 tggcgctggt cgggccagac gaaggcgagc aggcgctcgg cgtcgcggcg gatgtcgagc
     1621 gggcgcaggt caaccccgcg gcgcgactgg atacgcacgt cggcgggtgg cggcgacgcg
     1681 ccgctccact cgggctggag gtggaggggt gacgacatgt cgccggcggc ggcgctgccg
     1741 agcgtgtagc gatagcgatc gggaatgagg ttgagccccg cactggcgcc aagctcgagc
     1801 aggcggatcg gcagcggcca tgtcgcggcg acgaccagca gcccgctcat cagggcggcg
     1861 gcgcggccga cctcgttggt ttggggcggg tggtcgagcc aggcgagcag cgccggccct
     1921 tcatcctcca gcgtgcggcg aatggctgcc cacagcgtct cgtcgtccgg cgtggggttc
     1981 ggcggatagc aacgcgcgag cggggcaccg cttcggacca gggcatgaag gccgccgcag
     2041 aagcgcagcg gcagtccgtc ggcccagccg tccgggttgc cgggccagtc gagggtgcgg
     2101 cggcccactt cggtgctgcg gtcgagccgt tcgcccagca aggtgcacaa tttggcggtg
     2161 aagggtgagc ccatcgtcgc gcaccagccg gcctgggtgg cgaaggcggc gcgaaccgtg
     2221 gcctcgccgc cttcagtcgc ggagccgggc accggccttg gtcgcggcgg cgacgatccg
     2281 ggcggagatc gccttcaatt cctcgtcggt gaagctcttc tcgcccggct ggagcgtgac
     2341 ttcgacggcg agcgacttct gcccttcggg aacgcccggc cccgtgaaca tgtcgaacag
     2401 gccgacgccg acgatcgccg ccttgtcggc gccgcgcacc gcccgaagca gggcctcggc
     2461 gggcgtttcg gcatcgacca ggaaggcgaa gtcgcgcttc accgcctgca gcggaggcgg
     2521 cgcataggca gagcgccggt ggccggcgtc gcgccgctgc gggatcaggt cgaggaagat
     2581 ttcggcgacc accactggtc cgtcgaggcc gaacgccttc agcgtcgaag ggtggagctc
     2641 gccgaattct gcgagcgcgg tcttggggcc gaggcagagc cggccggagc ggcccggatg
     2701 gtggacaagc gaggcgtcgc ccagcacttg caaattgtcg accggagccc cggcggcggc
     2761 gaggatcgcc agcgcctcgg ccttggcgtc ataggcgtcg aagccttgcg ccttgccggt
     2821 gcgccagtgg cgcgggccgc gctcgccggc aagcacgagg ccgagcgcgg cgcgctcgcc
     2881 gtcggcgagg taacggcggc cgatctcgaa cagccgcacg ccggcggcgc cgcggtcgat
     2941 gttgcggcgc gcggcggcga gcaggccggg gaggagcgac gggcgcatcg ccttcatctc
     3001 ctcgctgatc ggattgtcga gcatccaggc gctgccgccg aacggcgccg cctcggcctc
     3061 ggcgacgaag ctccaggtga ccgcctcatg gagcccgcgc gcggcggcgg cccggcgggc
     3121 gcggcgctcg atcagctgct cgggcgtagc ggtcggcctc gccacgccgg gcgcgcgctc
     3181 gagcggggtc gaagggactt tgtccatgcc ttcgatgcgg atcacttctt cgacgatgtc
     3241 ggccgcgccg atgacgtcac ggcgccatga cggcacggtg atttcccagg tttcttcaac
     3301 ggttaccccg aagccgagcc gctccagcgc cgcctgctgc cgatcctccg gcaccgcgag
     3361 tccgccgagc tccgcgcatc ggttggggga atagccgatg atccgcgcct cgatcggcgg
     3421 cgtgcccgcg cggaccggct cggagggctc gccgccggca agctcgatcg tgaaggcggt
     3481 ggcgagctga aggccggttt cgacgaaagc cggatcgacg ccgcgctcga aacgggtgcg
     3541 ggcgtcggag gtgaggccga gcttctggcc ggtgcgggcg atatgctcgg gatcgaaata
     3601 ggcgcattcg atcaaaatgt cggtggtggc ttcggtgacg cccgattcct cgccgcccat
     3661 gatgccgccg atatcgtgga catgggcctc gtcggcgatc accgtcatcg tctcgtcgag
     3721 cgcataggtc ttgccgttga gcgccagcac ctgctcgccg gggcgagcgc ggcgggcgga
     3781 gatcgcgcct tcgagtttcg aaagatcgta gacgtggagc ggccgcccgt agctcagcat
     3841 gatgtagttg gtcatgtcga cgagggcgct gatcgggcgc tggccgaccg ccttcagcct
     3901 ttgctgcagc caggccggcg aagcgccgtt cttcacgccg cggaccacgc gcccgtagaa
     3961 agcggggcag ccctgcggat catcggtgcg gatctcgatc gggcagggga agcggccggg
     4021 gacctccgcc accgcgatcg gcttcagcgt gccgaggccg gctgcggcga ggtcgcgggc
     4081 gatgccgtgg acgcccatcg catcctgacg gttcggcgtg atcgcgacgt cgatgaccgg
     4141 gtcgtcgagg cccgcccatt gcgggtagct ggcgccgatc ggagcgtcgt cgggaagcgc
     4201 aatgatgccg tcatgctcgt cgccgagctc gagctcgcgc accgagcaca tcatcccccg
     4261 gctttcgacg ccgcggatcg aggcaatctt caaggtgagg tcgctgcccg ggacgtaggc
     4321 gcccggcgct ccgaacacgc cgagcatgcc ggcgcgggcg ttgggcgcgc cgcagacgac
     4381 ctgcaccggc tcgccggcgc cggtgtcgac ggtgagcacc tgcagcttgt cggcctgcgg
     4441 gtgccgctcg gcggtcagca ccttggcgat ggtgaagggc gcgagcttct cggccgggtt
     4501 ctccacgccc tcgacctcga ggccgacgcc ggtcagcgcc tccacgatct cgtcgagcgc
     4561 ggcggaggtg tcgagatgct ccttcagcca ggaaagggtg aatttcattt cagcgtccgt
     4621 tcgtgtcgag cgaagtcgag acatcccgcc gagcgaagcc gaggcggcaa gtcggcgaca
     4681 gtctcgcctc cgctcgacag ggcccctcga ctgcgctcgg ggcgaacggg gcaaggcgca
     4741 gcatcagggg cgaccggctc atgccccgac tcctccgctg agcgtcggca cgtcgagcgc
     4801 ggagaagccg taatgcttca gccaccggag atcgccgtcg aagaaggcgc gcaaatcgtc
     4861 catgccatat ttgagcatcg ccaagcgatc gatgccgcag ccgaaggcga agccctgcca
     4921 ttcgtccgga tcgagcccgc aattggcgag cacgcgcggg tggaccatgc cggagccgag
     4981 cagctccatc cagccttccg tgccgccgag gacgcggcgg cccttcacca tcgtgtagcc
     5041 gacatcaact tcggccgagg gctcggtgaa ggggaaatag ctcggccgca tcctcagcac
     5101 gatgtcgtcg cgttcgaaaa acgccttgag gaacgtctcc agcgtccatt tgaggtggcc
     5161 gagatggatg cccttatcga tcaccagccc ctcgacctgg tggaacatcg gcgtgtgggt
     5221 ggcgtcgctg tcggaacggt aggtgcggcc gggggcgatg atgcggatcg gcggcggctg
     5281 gctctgcatg gcgcggacct gcaccggcga ggtgtgagtg cgcagcagca tccggtgccc
     5341 ggcgccgtcg cttcggtcga aatagaaagt gtcgtgcatc gcccgcgcgg ggtggctctc
     5401 cgggatgttg agcgcggtga aattgtgcca gtcgtcctcg atctcggggc cggtcgcgac
     5461 cgagaagccg aggtcggcga agatttcggc gagctcgtcc atcacctggc tgaccggatg
     5521 gacgctgcct tgcagccccg gcggcaccgg caaggtcatg tcgagcatct cggtggccag
     5581 ccggcgatcg agctcggccg cctccagcgc cgccttgcgc gacgctatgg cgtcagccac
     5641 gcgctctcga agcgcgtgga tgcgcgggcc cccctgctgc cgctgctcgg ggctcatccc
     5701 gcccagcgtc ttcaagagct gcgtgaccac gccttgcttg cccagcgccg ccacccgctc
     5761 cgcctccagc gccggcaggt cggtggccgc gtcgattcgg cccagcagct cgcggctcat
     5821 aagttcgaga tcggacatgg ccggcgctct agacgacata cgcgggcgaa tggaaccctc
     5881 gccgggggcg ccaaagctga cgaagttggg gcgttggcag gcgattcgcg tcagctttgt
     5941 cagttttggc gggccgcgcc ggtcctcgcc cggggtggag aggaggggat gcggttcgcc
     6001 catcgcgcca gccaagcatg cgaaatccta ttttggaagc gtcgggaccg gggctgagca
     6061 ggcggctcag gccattgctg agacgaggcc gagatgttcc acgaagggat cgaagtctcg
     6121 atcctggaag aggagcggcg tcgaatgcag aatgcaatag gtggcgatga gcgcatcgat
     6181 ggtcttgcgg accgtgatgc cgcgggcgcg caggagccgg taattggccg cggcctgcaa
     6241 cgccacctcg cgtcctccgg ttcgaacgaa gaccggcaac gagaggaggc gaagagcgtc
     6301 agcaaactcg gagtcgcgat cgaacccctg cagcacctcg acgaggatga gatcggcaac
     6361 ggccaccgct tgtcggccga gcaggccgtt gagaacgtcc gtgtgcggcg aaagcgtccc
     6421 ccgaaaaaag tcgatccaca ctccggaatc gaccaggatc attagtcgag ccgcatcgtc
     6481 tcaaggtcac cttgccagtg aagcttgccg cgccagttcc tgagttcgcc ctgtcgcccc
     6541 aactggacga ggccgcgcag ggcgatctct accgcctcgc gcttggtcct gacgccggtg
     6601 agcttcatcg cttcggccag caaagcgtcg tcaatgtcga tgttggtccg catgtgtata
     6661 atttagccta atcgtacaca tcggtcaatc ttcacccccc gaacgggtaa ggcgagatcg
     6721 tcttttgcat ctcgttgacc atcaaggtcc gcccggcggg cgcggcggcg cgttgggggc
     6781 agcggatgcg ggggcagatg gtgcaggcgg ggccgatcgg ggtgacatag ggctcagcca
     6841 aatcgaggcc gtcggcatag acgagcttgg gcgcgaactt gacgtcgcag ccgaggccga
     6901 tggcgagctg gctgctctcg cgcgggtcga gccggacggc gcgctcgacc gcgcggctga
     6961 cggtgaagaa gcgctggccg tcgggcgtct cgacgatctg ggtgatgact cgttcgggcg
     7021 tgcggaaggc ggcgtggagg ttccagcgcg ggcaggtgcc gccgaagcgc gagaagggga
     7081 agctctcgcc ggcgaagcgc ttggagatgt tgccggcggc gtcaacgcgc agcatgaaga
     7141 agggaatgcc gcgcgcgccg gcgcggccga gcgtggtcag gcggtgggcg acctgctcgg
     7201 cgctggcgcc gaactccgcg cacagccggt cgatatcgta gcggtggcgc tcggccgcgc
     7261 cgaggaaggc cgcataaggc atcaggatcg cgccggcggc ataattggcg aacgacatgt
     7321 ggagcaggcg ccgggtcggc atgtcgggcg gagccgcgct ctccagcatc cgcgcgagca
     7381 gcgggcggaa ctcgaacaag gcgacctgat aggcgaccgc gaaggcgcgg ctttccgggc
     7441 gcagcagctc ggacagcatc agccgccggc ggtgatagtc gtaatgctgg ctggtgccgt
     7501 cgagcaattc gggcggcacc acccgcgctt cgacgccgaa gccttccttg aggcgccggg
     7561 cgagcgcggc gaagatgcct ggccggtcgc cgagcgcgcc ggcgagcgtc tcggcggctt
     7621 cctcgatata gccgaaataa ttgcgctggg cctggatata gtcgcgcacc caggtttcgg
     7681 gcgtgacgtg cgaagtctcg ccgtcgggcg ccgcgccggg cggcgcgttc cggcgctcga
     7741 ctagcgcagt gtaaaggcgg gagatggcgt cggcgaccga aggcgcgttg tcggcaacct
     7801 cgagcaattc gtagcgtggg ataccgagat cgccgaacat cgggtcggcg aagatctcgg
     7861 cgaggtcctc cgtgccggtg ccctcccctt cggcggtgaa gcttttgagg tcgacctcat
     7921 aggcctgggc gaggcggagc agcacctgcg ccgtcaccgg ccgctggttg cgctcgagat
     7981 ggttgaggta gctgggcgac atgcccagtt cgcccgccat cgcgctctgg ctgacgccga
     8041 gctcgcgcct gaggcggcgc aggcgcccgc ccagatagag tttacgatcg gcagccacgg
     8101 ctgttatatt gtcaagaatt tacaagtgtc gcaagtcgcg ctgtgacaaa gcgacccttt
     8161 tcgatgccac cgccgcggcc gatttgaggt tcaatcaccc ggaactagcg aaacagggtg
     8221 attctcagat gagtgatttc agcagcgtcg ttccggcccc cgcggaccgc ttccagggca
     8281 tcagccgccc ctatgacgta gccgaggtgg agcggctccg cggaagcgtg ccgattgagc
     8341 atacgctcgc caaacgcggc gccaaccggc tgtgggagct cctgaagagc gaggattatg
     8401 tccatgcact cggcgcgctg tcgggcaacc aggcgatgca gatggtccgc gcgggcctga
     8461 aggcgatcta tctttccggg tggcaggtgg cggcggacgc caacacggcg ggcgccatgt
     8521 atccggacca gtcgctctac ccggccaatg ccggaccgga attgtgccgg cgaatcaaca
     8581 agacgctgca gcgcgccgac cagatcgagc atgccgaggg cggcgcgcag cgcgactggt
     8641 tcgtgccgat cgtggccgat gccgaggcgg ggttcggcgg cccgctcaac tgcttcgaga
     8701 tcatgaaggc ctatatcgag gcgggcgcgg ccggcgttca tttcgaggac cagctcgcgt
     8761 ccgagaagaa gtgcggccat ctcggcggca aggtgctgat cccgacccag gcgcatatcc
     8821 gcaacctcga cgcggcgcga ctggcggccg acgtgtgcgg cgtgccgacc gttctcgttg
     8881 ctcgcacgga cgccgagagc gccaagctca tcacctgcga cgtcgacggg cgcgaccatg
     8941 aattcctcac cggcgagcgc acgcccgagg gcttcttccg cctgagggag gggacgggcg
     9001 tcgaccattg catcaggcga ggcctcgcct acgcgcctca cgcgg
//
LOCUS       DASVRK010000013         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_126977, whole genome shotgun sequence.
ACCESSION   DASVRK010000013 DASVRK010000000
VERSION     DASVRK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126977"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VF589_00585"
     CDS             <1..285
                     /locus_tag="VF589_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX8256098.1"
                     /translation="PLATVNQLAAAVSARDPFRLAGLAPLVTVSGSLIIALALAEGAM
                     SLEDGWAAATLDEAWQAEQWGEDAEASAALDARRKDFGAAARLLDLLREP"
     gene            complement(298..930)
                     /gene="gmk"
                     /locus_tag="VF589_00590"
     CDS             complement(298..930)
                     /gene="gmk"
                     /locus_tag="VF589_00590"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX8256099.1"
                     /translation="MGTRSQVKRRGLLFVLSSPSGAGKSTIARMLLARDDGISLSVSA
                     TTRPIREGEVDGRDYHFITDAEFDRMVASGAFLEWALVFGRRYGTLLSEVSQSIQNGC
                     DVLLDIDWQGTQQLKQVDPDIVRVFILPPSMAELERRLRTRGTDSDEVIRGRMERAAS
                     EISHWAEYDYVLINEDADICLGKVHSILEAERLKASRRTGLHDFVRSLIG"
     gene            987..1526
                     /locus_tag="VF589_00595"
     CDS             987..1526
                     /locus_tag="VF589_00595"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX8256100.1"
                     /translation="MEKFVALLRGINVGGRNRIGMADLRALCGGLGWGEVETYIQSGN
                     VVFMAEAAPAELEAALEAAIQQRFAMTIPVIVRSASGWPAYVAGNPFPEAAAAEPNRL
                     MLMLSKGPPQPDAAAALQERARDGERVAAAGDALWVHFTSGSGTSRLSPALFDRLVGS
                     PVTARNYRTAVKLQELLAG"
     gene            1523..2083
                     /locus_tag="VF589_00600"
     CDS             1523..2083
                     /locus_tag="VF589_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HEX8256101.1"
                     /translation="MSGIAKRHWLIALAIVALAAAILLAAGRPPICTCGRIDLWAGAV
                     QSADNSQHLADWYTPSHIIHGFLFYGLGWLFLRRNPIGERFIGAVAIEAAWELLENSQ
                     MIIDRYREATFALGYNGDSVINSVADIGWMALGFLLARRLPVAATIALAIGFELLTLW
                     TIRDNLTLNVLMLLAPVDAIREWQAA"
     gene            complement(2089..2259)
                     /locus_tag="VF589_00605"
     CDS             complement(2089..2259)
                     /locus_tag="VF589_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256102.1"
                     /translation="MSGAADGTAPGDEAPPGTPGTGENLCRHCNGTGEHDDAPCPVCD
                     GTGKVTEGIGGA"
     gene            complement(2256..2792)
                     /locus_tag="VF589_00610"
     CDS             complement(2256..2792)
                     /locus_tag="VF589_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8256103.1"
                     /translation="MAKYLLTAAAAALLTTFAGSAGAMAPPPPSGGSADMTPKDRAGY
                     MRMAASGDLYEIQSSQIAVQRSQNPAVRKFAQMMIEHHTMTSKQLMTAAKGAGANPPP
                     PQLMPMHAEMIAQLQGANGEGFDQLYMSQQTQAHKMALELHRNYANDGDTAALQSTAR
                     TATPIVERHLAAAQQLQR"
     gene            complement(2822..3460)
                     /locus_tag="VF589_00615"
     CDS             complement(2822..3460)
                     /locus_tag="VF589_00615"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8256104.1"
                     /translation="MRHVLLSAAAASAALLAGCATDYGYDRGYGAGEYDQGPPPGGYD
                     EGPPPGGYDQGPPPGGGYDAAPMPAMPLDRIGYTEMAASSDLFEIESAQMALDRGRHS
                     AVRQFSQMILRDHRMMSQQLMAAANAAGVRPMRPRLLPIHADMLARLQAGDGRDFDDR
                     YARIQVQAHEMALALHDNYARGGDAASLRAVAGSAVPIIRHHLDMARRWPVR"
     gene            complement(3548..4468)
                     /locus_tag="VF589_00620"
     CDS             complement(3548..4468)
                     /locus_tag="VF589_00620"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX8256105.1"
                     /translation="MLFDFAPAASHGPARAHPETDIRWGVDSEYGRLTDVMLSAPPHL
                     EVLPCNSVAVEALANGLACCPVAAETQHRALARTLEAEGVSCHFVPPSEDMPDLSFTR
                     DAALMTPWGLLQLVPAVAHRAAEAAYVRRTAESWGVPILGRLPEGRAEGGDLCVLRPG
                     VVVIGYSGERTDEAGASALARLFEARGWRAIRYRFDPHFLHLDTHFTTVDRNRAVACT
                     EAIDPAFLGQLEELGIDLVPVSYDEVQRLGGNILSLGDGRVLSSADNHRLNGQLERLG
                     YRVLPVEIDQFVRCGGGIHCLTLPLARTPG"
     gene            complement(4608..>5185)
                     /locus_tag="VF589_00625"
     CDS             complement(4608..>5185)
                     /locus_tag="VF589_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX8256106.1"
                     /translation="DALVFTQGALASLAAGLFKIANDVRLMGSGPRSGLGEISLPENE
                     PGSSIMPGKVNPTQAEMLTMVAAQVIGNHVSVTVGGMQGHLELNVFKPLIGAALLRSI
                     DLLSIGMASFTRRCVDGLQPNARRIAELVERSLMLVTALAPEIGYDAAAKIAKHAHER
                     GLTLKEAGLELGLVDEATFDRVVRPEAMLGR"
BASE COUNT          759 a         1778 c         1800 g          848 t
ORIGIN      
        1 ccgctcgcga cggtgaacca gctggccgcg gcggtgtcgg cccgcgaccc gttccgtctc
       61 gccgggcttg ccccgctggt gacggtgagc ggctcgctga tcatcgcgct tgccttggcg
      121 gagggcgcaa tgagcctgga ggacggctgg gcggccgcga cgctcgacga agcctggcag
      181 gccgaacaat ggggcgagga tgccgaagcc tccgccgcgc tcgacgcgag gcgcaaggat
      241 ttcggcgccg ccgcccgcct gctcgatctg ctcagggagc cttagccgcg tcgctcctca
      301 gccgatcagg ctgcggacga agtcgtgaag ccccgtccgg cgcgacgcct tcagccgctc
      361 ggcctccagg atcgagtgga ccttgccgag gcagatatcg gcatcctcgt tgatcagcac
      421 ataatcatat tcggcccagt ggctgatctc ggacgccgcg cgctccatgc gcccccggat
      481 gacctcgtcg ctatcggtgc cgcgggtgcg cagccgccgt tcgagctcgg ccattgacgg
      541 cggcaggatg aagacccgga cgatgtcggg gtctacctgc ttaagctgct gggtgccttg
      601 ccagtcgatg tcgagcagca cgtcgcagcc attctggatc gactggctga cctccgacag
      661 caaggtgccg tagcgccggc cgaataccaa agcccattcg aggaacgcgc cgctcgccac
      721 catccggtcg aactcggcgt cggtgatgaa atgatagtcg cggccatcga cttcgccttc
      781 ccggatcggg cgcgtggtgg cggagacgga gagcgagatg ccgtcgtccc gcgcgagcag
      841 catccgggca atggtcgatt tgcccgcgcc gctcgggctc gacagcacga acagcagccc
      901 gcgtcgcttg acctgacttc tcgtccccat ggccgctact ggcgcgggcg gcgccgatgc
      961 gtcaagagtg gaaggcggga gagggcatgg agaagttcgt cgcgctcctc aggggcatca
     1021 acgtcggcgg ccgcaaccgg atcggcatgg ccgatctgcg cgcgctttgc ggcgggctcg
     1081 gctggggcga ggtggagacc tatatccaga gcggcaacgt cgtcttcatg gccgaggccg
     1141 cgcccgccga gctcgaggcg gcgctggagg cggcgatcca gcaacggttc gccatgacga
     1201 ttcccgtcat cgtgcgctcc gcctcaggct ggcccgctta cgtggccggc aacccctttc
     1261 ccgaagcggc ggcggcggag ccgaaccggc tgatgctgat gctgtcgaaa gggcctccgc
     1321 aacctgatgc cgccgccgct ctccaggagc gggcacgcga cggggagcgg gtcgcggcgg
     1381 cgggcgatgc gctatgggtg cattttacct cgggctccgg cacgtcgcga ttgtcgccgg
     1441 cgctgttcga ccggctggtc ggatcgccgg tgaccgcgcg caactaccgg accgcggtca
     1501 agcttcagga gctgctggcc ggatgagcgg gatcgcaaag cgtcactggc tgatcgcctt
     1561 ggccatcgtc gccctggcgg cggcgatcct gctcgccgcg ggccgcccgc cgatctgcac
     1621 ctgcggccgc atcgacctgt gggccggcgc ggtgcagagc gccgacaaca gccagcacct
     1681 ggccgactgg tacacgccca gccacatcat ccacggcttc cttttctacg gcctcggctg
     1741 gctctttctc cgccggaacc cgatcggcga gcgcttcatc ggcgccgtcg cgatcgaagc
     1801 cgcctgggag ttgctggaga acagccagat gatcatcgac cgctaccgcg aggcgacctt
     1861 cgcgctcggc tataatggcg acagcgtgat caactcggtc gccgacatcg gctggatggc
     1921 gctcggcttc cttctcgccc gccggctgcc ggtcgccgcc accatcgccc tcgccatcgg
     1981 cttcgagctg ctcacgctgt ggaccatccg cgacaatctg acgctcaacg tcctgatgct
     2041 gctcgcgccc gtggatgcga tccgggaatg gcaggccgcc tgagggcctc aggcgccgcc
     2101 aattccctcg gtcaccttgc cggtgccgtc gcacaccggg cagggcgcgt cgtcatgctc
     2161 gccggtgccg ttgcagtggc ggcacagatt ctcgccggtg ccgggcgtgc ccgggggcgc
     2221 ctcgtccccg ggcgcggtgc cgtccgcggc cccgctcatc gctggagctg ctgggccgcg
     2281 gctaggtgcc gctcgacgat cggcgtcgcc gtccgggcgg tcgactggag cgcggccgta
     2341 tcgccgtcat tggcataatt gcggtgcagc tccagcgcca tcttatgcgc ctgcgtctgt
     2401 tggctcatgt agagctggtc gaagccttcg ccgttggcgc cctggagctg ggcgatcatt
     2461 tcggcgtgca tcggcatcaa ttgcggcggc ggcgggttcg cgcccgcgcc cttggcggcg
     2521 gtcatcagtt gcttgctggt catcgtatga tgctcgatca tcatctgagc gaacttgcgc
     2581 accgccgggt tctggctgcg ctgcacggcg atctgggagg attggatctc gtaaagatcg
     2641 cccgacgcgg ccatgcgcat atagccggcc cggtccttgg gcgtcatgtc ggccgatccg
     2701 cccgatggcg gcggcggcgc catggcgccg gcactgcccg cgaaggtggt cagcagggcg
     2761 gcggccgccg cggtgagaag atatttcgcc attgtgggtt ttcctttcaa ttttgcttgg
     2821 gtcaacggac aggccagcgt cgggccatgt cgagatggtg gcggatgatc ggcaccgcgc
     2881 tgcccgcgac ggcgcgcagc gaggcggcat cgccgccgcg ggcgtaattg tcgtgcagcg
     2941 ccagcgccat ctcgtgcgcc tgcacctgga tacgggcgta gcggtcgtcg aaatctcggc
     3001 cgtcgccggc ctggaggcgg gcgagcatgt cggcatggat cggcagcagc cgcggccgca
     3061 tcggccgcac cccggcggcg ttggcggccg ccatcagttg ttgcgacatc atccggtggt
     3121 cgcggaggat catctgcgag aattgccgga ccgcggagtg ccgcccccgg tcgagcgcca
     3181 tttgcgcgga ttcgatctcg aacaggtcgc tggaagcggc catctccgta tagccgatcc
     3241 ggtcgagcgg catcgcgggc atcggcgcgg cgtcatatcc gccgcccggc ggcgggccct
     3301 ggtcatagcc gcccggcggc ggtccttcgt catagccgcc cggcggcggc ccctggtcgt
     3361 attcgccggc cccgtagccg cggtcatatc cgtagtccgt cgcgcacccg gccagcaggg
     3421 cggccgaggc cgccgcggcc gatagcaaaa catgtctcat ctgcgatcct ccgaaacgct
     3481 tcagaggaaa ccggcgccgg cgagcgggag ttccgctaaa gccgttatcc taacgtgggt
     3541 tagcgaatta gcctggggtc cgggcgagcg gcagcgtcag gcaatggatg ccgcccccgc
     3601 agcgcacgaa ctggtcgatc tcgaccggaa gcacccggta gccgagccgc tcgagctgtc
     3661 cgttcagccg gtgattgtcg gccgacgaaa gcacccggcc atcgccgagg ctgaggatgt
     3721 tgccgcccag ccgctggacc tcgtcatagc tcaccgggac gagatcgatg ccgagctcct
     3781 cgagctggcc gaggaaggcc gggtcgatcg cttcggtgca ggccacggcg cggttgcggt
     3841 cgacggtggt gaaatgggtg tcgaggtgca ggaaatgcgg gtcgaaccgg tagcgtatcg
     3901 cccgccaccc gcgcgcttcg aacagccgcg ccagcgcgct cgcgccggct tcgtcggtgc
     3961 gttcgcccga atagccgatg acgacgacgc cgggccgcag cacgcatagg tcgccgccct
     4021 cggcgcggcc ttcggggagc cggccgagga tcggcacgcc ccagctctcc gccgtccgcc
     4081 gcacataagc cgcttccgcc gcccggtgcg ccaccgccgg gaccagctgg agcaggcccc
     4141 acggagtcat caaggccgcg tcgcgggtga agctgaggtc gggcatgtcc tccgacggcg
     4201 gcacgaagtg gcagcttacc ccctcggcct cgagcgttcg ggcgagcgcc cggtgctggg
     4261 tctcggccgc caccgggcag caggcgaggc cgttggccag cgcttcgacc gcgaccgaat
     4321 tgcagggcag cacttcgagg tggggcggcg cggacagcat gacgtccgtc agccggccat
     4381 attcggagtc gacgccccag cggatgtccg tttccgggtg ggcgcgtgca gggccgtggg
     4441 aggcagccgg cgcgaagtcg aacagcatcg gatctctctg gttgcgccgc ttacatccgg
     4501 ctgaggcaaa gcccgaaccc cggactaccg ttccggttcc cgaacgaagc gcggtccgtc
     4561 gggcctaacg gatggggagg tcgctgccgc gaaaccagcc ccggccctca ccgccccagc
     4621 atcgcctccg ggcgcaccac ccgatcgaag gtggcttcgt cgaccagccc gagttccagt
     4681 cccgcctcct tgagggtcag ccccctttcg tgcgcatgct tggcgatctt cgccgccgcg
     4741 tcatagccga tctcgggcgc aagcgcggtc accagcataa ggctgcgctc gaccagttcg
     4801 gcgatgcgcc tggcgttggg ctggagtcca tcgacacagc gccgagtgaa actggccatg
     4861 ccgatgctca gcaggtcgat cgagcggagc agcgcggcac cgatcaaggg cttgaagacg
     4921 ttgagttcga ggtgcccctg catgccgccg acggtgacgc tcacgtggtt gccgatcacc
     4981 tgcgcggcga ccatggtcag catctcggcc tgggtcgggt tgaccttgcc gggcatgatc
     5041 gaagagccgg gctcattctc gggcagcgag atctcgccca gtcccgagcg cggccccgag
     5101 cccatcagcc ggacatcgtt ggcgatcttg aacagccccg ccgcaaggct cgcgagcgcc
     5161 ccttgcgtga acacgagggc atcgt
//
LOCUS       DASVRK010000014         5805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_500413, whole genome shotgun sequence.
ACCESSION   DASVRK010000014 DASVRK010000000
VERSION     DASVRK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500413"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VF589_00630"
     CDS             <1..673
                     /locus_tag="VF589_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256107.1"
                     /translation="DTMTGGDGNDTYIVDNIRDVAIETNADRATGGIDTVKSSVSFTL
                     GANVEKLTLTGGQDVDGTGNALGNTIVGNIGDNTLLGGGGNDVITGGRDGDEIDGGAG
                     ADRLVYGSALDSTSVNFDTITAFNFDEDKIDLPNSVPAPQFVAVVEQGRLDEDTFDAD
                     LAAAVGGALQSGTAAVFVPSSGDFEGKTFFVLDGNGVAGYQAGGDYVVRVEDSAIPTH
                     SDFFV"
     gene            complement(797..1729)
                     /locus_tag="VF589_00635"
     CDS             complement(797..1729)
                     /locus_tag="VF589_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8256108.1"
                     /translation="MKPIRIGIIGYGKIARDQHVPAIARLPRFELAATVSRQGKGAEG
                     LPCFTSHGAMIAEAGLEAVAICTPPAARYHIARECIAAGLHVLLEKPPAVTLGEIEDL
                     ACLAEAQGISLFATWHARFNPAVAAAAQALAGQRIASMEIVWREDVRKWHPGQQWIWE
                     PGGFGIFDPGINALSIATAVFPGPLFMRAAELTYPENKQAPIAAALRLFSPAADADMT
                     AEFDWRHSGGEAWDIRVRTADGSVVELKEGGSRLIIDGEPRTAGGAGEYPLIYERFLD
                     LIDARRSDVDLRPLRLVADAFLTGRRAASEPFQD"
     gene            complement(1726..2355)
                     /locus_tag="VF589_00640"
     CDS             complement(1726..2355)
                     /locus_tag="VF589_00640"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX8256109.1"
                     /translation="MNAVADFQHYLAECPLVAIIRGVTPDEAEAIGEALYRGGIRIIE
                     VPLNSPQPLVSIRRLARALGDRALIGAGTVLDPVDVARVAEEGGRLIVSPNMDEAVIA
                     ATVEAGLVSSPGYFTPSEAFAALRAGAHALKLFPAEAASPAVVKGQRAVLPKEVPLLV
                     VGGIAPDNMQPWLAAGANGFGLGSGLYKPGQSPDEVERQARAYVAGLGR"
     gene            complement(2352..3239)
                     /locus_tag="VF589_00645"
     CDS             complement(2352..3239)
                     /locus_tag="VF589_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX8256110.1"
                     /translation="MRWAEGFIAVDWGTTNRRGYAIGPDGRMTGEFEDDQGILSVPKD
                     GFPAAVAELRAKLGDRPLLMAGMVGSNRGWVEAPYVSCPGGLPELAAGLKWVEPERIA
                     IVPGVCYAAGEAADVMRGEEVQILGAYGEGMIPSDATICHPGTHNKWISLEDGRIAAF
                     RTVMTGEMFNLLREHSILADLLQEDAEPGEAFEAGVRRGLEGDELTAELFSVRARVLL
                     GKARREDAASYTSGLLIGTDIRIGLRSAGSGAIVVMGRPELTELFAGALRIAGREARE
                     VDGEAAFLAGVKHLAELIQ"
     gene            3330..4208
                     /locus_tag="VF589_00650"
     CDS             3330..4208
                     /locus_tag="VF589_00650"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8256111.1"
                     /translation="MDEDKTARCIADVKAVLGEGPVWVEREQSLYWVDIKGRRIFRYA
                     LEGGELTEWPAPFRICSLAPRAEGGFIAGTEDGFAFVDPAANRFGLQFDPEPERATNR
                     FNDGKLDRAGRFWAGTMDDAEVEASGALYRLSPGAAPVRVDDGYKVTNGPAFSPDGRL
                     MYHNDSALQTTYVFDLDEAGEASGRRVFLSFGEGDGYPDGMTVDAEGCLWIAFWDGWC
                     LRRYAPSGELLQHYELPVQKPTSCAFGGPGLDHLFVTSASIGLDEKALAGQPEAGGLF
                     LLMPGVKGLADGLFAG"
     gene            4346..4855
                     /locus_tag="VF589_00655"
     CDS             4346..4855
                     /locus_tag="VF589_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX8256112.1"
                     /translation="MEQETLLALTADIVAAHVANNTVAVSDVPELVKRVHTALAGLGQ
                     VEPEPVQEKTPVVSIRASIKPDYLVCMECGRKQKTLKRHLQTAHGMTPDQYRTDYGLP
                     ANYPMVAPEYSKRRSEMANAIGLGRKRGDTRAAAPKPAARRGPNGTGGAPQPPARRGR
                     RKAAPKAPE"
     gene            5082..>5805
                     /locus_tag="VF589_00660"
     CDS             5082..>5805
                     /locus_tag="VF589_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX8256113.1"
                     /translation="MFATGIENSIPTINNGRTRIDQMESCAHYDRWREDFHLVQEIGI
                     HFLRYGPPIHRTYLAPGRYDWEFADIAFAELKRLDITPIVDLCHFGVPDWIGNFQNPD
                     FPRLFASYAAAFAERFPWVQLYTPVNEMFICAIFSAKYGWWNEQLKTDRGFVTALKHI
                     VKANVLAMTEILKRRPDAIFIQSESSEYFHADSPAAIGPAEVMNSRRFLSLDLNYGRR
                     VDSEMYEYLLGNGMTKDEYDFFL"
BASE COUNT          971 a         2057 c         1828 g          949 t
ORIGIN      
        1 cgacacgatg accggcggcg acggcaacga cacctatatc gtcgacaata tccgcgacgt
       61 ggcgatcgag accaatgccg accgcgccac cggcggcatc gacaccgtca agagcagcgt
      121 cagcttcacg ctgggcgcca atgtcgaaaa gctcaccctc accggcgggc aggacgtcga
      181 cggcaccgga aacgcgcttg gcaacaccat cgtcggtaat attggcgaca acacattgct
      241 cggcggcggc ggcaacgatg tgatcaccgg cggccgcgac ggcgacgaga tagacggggg
      301 cgccggcgcg gaccgcctgg tctacggctc ggcgctcgac tcgacgtccg tcaatttcga
      361 cacgatcaca gccttcaact tcgacgaaga caagatcgac ctgcccaatt cggtcccggc
      421 gccgcaattc gtcgccgtgg tcgagcaggg ccggctcgac gaggacacgt tcgacgccga
      481 cctcgccgcc gccgtcggcg gcgcgctgca atcgggcacg gccgcggtgt tcgtgccgag
      541 ctccggtgat ttcgaaggca agaccttctt cgtgctcgat ggcaatggcg tggccggtta
      601 ccaggccggc ggcgactatg tcgtccgcgt cgaggattcg gcgattccga cccacagcga
      661 cttcttcgtc tagggttcgt cgaggtcggg atgaaccgtc atgccagcga aagctggcat
      721 ctcacttccc ttagcggggg tacgaaagaa gggagattcc agctttcgct ggaatgacgg
      781 caccggtgga ccgagcttaa tcctggaacg gttcggacgc cgcccgcctt ccggtgagga
      841 aggcgtcggc gaccagccgc agcggccgca aatcgacgtc cgaacggcgg gcgtcgatca
      901 ggtcgaggaa gcgctcgtag atcagcggat attcgcctgc cccgccggcc gtgcgcggct
      961 cgccgtcgat tattagcctc gatccgcctt ccttcagctc caccacgctg ccgtccgcgg
     1021 tccgcaccct tatgtcccaa gcctcgccgc cgctgtgacg ccagtcgaac tcggccgtca
     1081 tgtcggcgtc ggcggccggg ctgaacagcc gcaacgccgc cgcgatcggc gcctgcttgt
     1141 tctcgggata ggtcagttcc gccgcccgca tgaacaaggg cccggggaag accgcggtcg
     1201 cgatcgacaa ggcgttgatg cctggatcga agattccgaa gccgcccggc tcccagatcc
     1261 attgctggcc cggatgccat ttgcgcacgt cctcgcgcca cacgatctcc atggaggcga
     1321 tgcgctgccc cgccagcgct tgcgccgccg cggcgacggc ggggttgaag cgcgcgtgcc
     1381 aggtcgcgaa caggctgatg ccctgggcct cggcaaggca ggcaaggtcc tcgatctcgc
     1441 ccaaagtcac cgccggcggc ttctcgagca gcacgtggag gcccgccgcg atgcactcgc
     1501 gggcgatgtg gtagcgcgcg gccggcggcg tgcagatcgc caccgcctcc agccccgcct
     1561 cggcgatcat cgcgccatgg ctggtgaagc agggcagacc ttccgcgccc ttgccttggc
     1621 ggctgacggt ggcggcgagc tcgaaccgcg gcagccgcgc gatcgccggc acatgctggt
     1681 cgcgcgcgat cttgccatag ccgattatgc cgatccggat cggcttcacc ggccgagccc
     1741 ggcgacatag gcgcgcgcct ggcgctcgac ctcgtcgggc gactgaccgg gcttgtagag
     1801 gcccgaaccg agtccgaagc cgttcgcccc cgccgccagc cacggctgca tattgtccgg
     1861 cgcgatgccg ccaacgacca gcaaaggcac ctccttgggc agcaccgccc gctgcccctt
     1921 taccacggcc ggcgaggcgg cttcggcggg aaacagcttc agcgcatgcg ctcccgcgcg
     1981 cagcgccgcg aacgcttcgg acggcgtgaa atagccgggc gaggaaacga ggcccgcctc
     2041 caccgtggcg gcgatcaccg cctcgtccat attgggcgag acgatcagcc ggccgccttc
     2101 ctccgcgacg cgggcgacgt ccacgggatc gagcaccgtg cccgccccga tcagcgcgcg
     2161 gtcgccgagc gctcgcgcca ggcgccggat gctgaccaga ggctgcggcg agttcaaggg
     2221 cacttcgatg atccggatcc cgccccggta gagcgcttca ccgatcgcct cggcttcgtc
     2281 gggagtcacg ccccggatga tcgccaccag cgggcattcc gcgagataat gttggaagtc
     2341 cgccaccgcg ttcattggat cagctccgcc agatgtttga cgccggccag gaatgccgct
     2401 tcgccgtcga cctcgcgcgc ctcgcggccg gcgatgcgca gcgcccctgc gaacaattcg
     2461 gtcagctcgg gccggcccat taccacgatc gcgccgctcc cggcggagcg caggccgatg
     2521 cggatgtcgg tgccgatcag cagcccgctc gtataggaag ccgcgtcctc gcgccgcgcc
     2581 ttgccgagca gcacccgcgc ccgcacggaa aacagttcgg cggtaagctc gtcgccctcg
     2641 agcccgcgcc ggacgcccgc ttcgaacgcc tcgcccggct ccgcatcctc ctggagcagg
     2701 tcggcgagga tgctgtgctc ccgcaggagg ttgaacatct cgcccgtcat cacggtgcgg
     2761 aaggcggcga tgcggccgtc ctcaaggctg atccacttat tgtgcgtgcc gggatggcag
     2821 atggtcgcgt cggacgggat catgccctcg ccataggcgc ccaatatctg cacctcctcg
     2881 ccgcgcatca cgtcggccgc ctcgcccgcg gcgtagcaga cgcccggcac gatcgcgatc
     2941 cgttcgggct cgacccattt gaggccggcg gcgagctccg gcagcccgcc gggacacgag
     3001 acatagggcg cttccaccca gccgcggttc gatcccacca tgcccgccat cagcagtggc
     3061 cggtcgccga gcttcgcccg caattcggcc accgccgcgg gaaagccgtc cttcggcacc
     3121 gacaagatac cctggtcgtc ctcgaactcg ccggtcatgc ggccgtccgg cccgatcgcg
     3181 tagccgcggc ggttggtggt tccccagtcg acggcgatga aaccttcagc ccagcgcatc
     3241 ctgtcctctc atctcacccg cccaacgccc aagccctgat tgggtcccat ggcgcttgaa
     3301 ccccctgcgc ggaacgatag ggagaggcaa tggatgagga caagaccgcg cgctgcatcg
     3361 ccgacgtcaa ggccgtactc ggcgagggcc cggtgtgggt agagcgcgag cagtcgcttt
     3421 actgggtgga catcaagggc cggcgcatct tccgctacgc gctggagggc ggggagctca
     3481 ccgaatggcc ggcaccgttc cggatctgct cgctggcgcc gcgcgcggag ggcggcttca
     3541 tcgccggcac cgaggacggt ttcgccttcg tcgatcccgc cgccaaccgg ttcggcctgc
     3601 aattcgatcc cgaacccgaa cgcgccacca accgcttcaa cgacggcaag ctcgaccgcg
     3661 ccggccgctt ctgggcgggc acgatggacg atgcggaggt cgaggcgtcc ggcgctctct
     3721 accgtctgtc gcccggcgcg gcgccggtcc gggtcgacga tggctataag gtgaccaacg
     3781 gccccgcctt cagcccggac ggccggctca tgtatcataa cgattcggcg cttcagacca
     3841 cctatgtctt cgacctcgac gaggccggcg aagcttccgg ccgccgcgtc ttcctcagct
     3901 tcggcgaggg cgacggctat cccgacggca tgaccgtcga tgccgagggc tgcctctgga
     3961 tcgccttctg ggacggctgg tgcttgcgcc gttacgcgcc gtcgggcgag cttctccagc
     4021 actatgaact gccggtgcag aagccgacca gttgcgcgtt cggcggcccc gggctcgatc
     4081 atctgttcgt cacctccgcc agcatcggtc tcgacgaaaa ggcgctggcc gggcagccgg
     4141 aggcgggcgg tcttttcttg ctgatgccgg gcgtcaaagg tctcgccgac ggactttttg
     4201 ctggctgaac ttgcacttgg cggtcgccgt ctggcgccat tcagcatcgc cgatgcggcc
     4261 ggctgaaaga tcaagttgaa cttcgtccag atttgatcca cgaagatttc cgccgcgtgt
     4321 cggcagttaa ttgtggggat tgaaaatgga acaggaaact ctgctcgctc tcactgccga
     4381 tatcgtcgct gcgcatgtgg ccaacaatac tgtcgccgtg agcgacgtcc ccgaactcgt
     4441 gaagcgcgtc cataccgccc tcgcaggcct agggcaggtc gagccggagc cggttcagga
     4501 gaagacgccg gtcgtttcta ttcgcgcatc gatcaagccc gattacctcg tgtgcatgga
     4561 atgcgggcgc aagcagaaga ccttgaagcg ccaccttcag acggcgcacg gcatgacgcc
     4621 ggatcaatac cgcactgatt acggcctgcc cgccaactat ccgatggttg cgccggaata
     4681 ttcgaagcgc cgcagcgaga tggccaatgc gatcgggctc ggccgcaagc gcggcgatac
     4741 ccgcgcggcc gcgcccaagc ccgccgcccg ccgcggcccc aacggcactg gcggcgcccc
     4801 ccagccgccg gcccgccgcg gccgccgcaa agccgctccc aaggctcccg aataagcgcc
     4861 gctccgcccg ctgggctgag gaccgatctt gccctctctc cctgtcgggg gagagggtag
     4921 ggccgaaggc gcgcgcggct cagccgtggg ccgcctttgc aactttatcc gccccgactc
     4981 ttttttcccg caaccggtcg cacgcggatg gcgctcgtcg tctccgctcg ctaggcgccg
     5041 gctgccgcgc gccgccatgg gagggactca tcgctaattt catgttcgcg accgggatcg
     5101 aaaacagcat cccgaccatc aacaacggcc gcacgcggat cgatcagatg gaatcctgcg
     5161 cccattacga ccgctggcgc gaggatttcc acctcgtgca ggaaatcggc atacatttcc
     5221 tgcgctacgg cccgccgatc caccgcacct acctcgcgcc cggccgctac gattgggaat
     5281 tcgccgatat cgctttcgcg gagctgaaac ggctcgacat cacgccgatc gtggacctct
     5341 gccacttcgg cgtccccgac tggatcggca atttccagaa tcccgatttc ccgcgcctgt
     5401 tcgcttccta cgccgccgcc ttcgccgagc gtttcccgtg ggtgcagctc tacacgccgg
     5461 tcaacgagat gttcatttgc gcgatcttct cggccaaata tggctggtgg aacgagcagc
     5521 tgaagaccga ccgcggcttc gtcaccgcgc tcaagcacat cgtcaaagcg aacgtgctcg
     5581 cgatgacgga gatattgaag cggcgccccg acgccatctt catccagagc gaatcgtccg
     5641 aatatttcca cgccgacagt ccggccgcga tcggcccagc cgaagtgatg aactcacgcc
     5701 gcttcctgtc gctcgacctc aattacggcc gccgcgtcga cagcgaaatg tacgaatatc
     5761 tgctcggcaa cggcatgacc aaggacgaat acgacttctt cctcc
//
LOCUS       DASVRK010000015         6215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_127071, whole genome shotgun sequence.
ACCESSION   DASVRK010000015 DASVRK010000000
VERSION     DASVRK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127071"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF589_00665"
     CDS             <1..307
                     /locus_tag="VF589_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8256114.1"
                     /translation="AMTLPWLAGVIEVVTGALIALGLFTRPAAFLASGTMAVAYWMAH
                     APQGPFPVNNGGDAAILYCFVFLYLAFVGAGPWSLDAKRNPEANLGTGHGSNGVQTH"
     gene            complement(611..835)
                     /locus_tag="VF589_00670"
     CDS             complement(611..835)
                     /locus_tag="VF589_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256115.1"
                     /translation="MPHYYFHIGQGADRVEDREGMTLPDQEAAWYHAWRSAAEHEAAS
                     AGSARILQVEDELGVPVLEVPVSELLRLVA"
     gene            1044..1577
                     /locus_tag="VF589_00675"
     CDS             1044..1577
                     /locus_tag="VF589_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256116.1"
                     /translation="MQRQIVSAVFDNRAEAERALAQLRSAGVRDEAISLIARHEGHSG
                     HTGDDDGDSGDDTRTGLGIGLGAGALLGFGALLIPGVGPFIAAGALAETLGVTGGAIA
                     AGAAIGGAAGGLAGALKDHGVTDEDSTYYEDRINQGGVFVSVDTSKADLDAERAQDML
                     YRAGGHSASRARTADAL"
     gene            1629..2120
                     /locus_tag="VF589_00680"
     CDS             1629..2120
                     /locus_tag="VF589_00680"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEX8256117.1"
                     /translation="MIRSVNAQFESRAEAEAAAARLERSHLAPEDLRIDVLNSAVPAG
                     AEPGLFDRFADWLLPRQGRPRRFRVSANVDEPQADEAIRILHEEPVQPIILTGGGGFG
                     GGVVEMAETAEEIVVTKQAVVREEVVLTREATECAETVHDTLRHTEVEVKRIAPPPPP
                     RQS"
     gene            2124..3365
                     /locus_tag="VF589_00685"
     CDS             2124..3365
                     /locus_tag="VF589_00685"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX8256118.1"
                     /translation="MRWDDNLTADRPSFVTHLECSMTGERYEAGRLQGLSLAGRPLLV
                     RYDLARVREALTRRALEARPADLWRWRELLPVRAKENIVSLGEAATPLVRLERTGAAL
                     GAGELLVKDEGRLPTGSFKARGLGVAVAMAKELGVRRIAMPTNGNAGAALAAYASRAG
                     IETIVLCPDDTPEVNVREIAAQGARVYRVNGLIDDCGRIVAEGAAEGRWFDMSTLKEP
                     YRIEGKKTMGLELAEQLGWAVPDVIFYPTGGGTGLIGMWKAFAELEAIGLIGGERPRM
                     VAVQAEGCAPIVKAWQEGEEHAPRWEDAHTVAAGIRVPRAVGDFLILRAVRESGGFAM
                     AVSDAALTQAVEDAARDDGLLLCPEAGATLAAWRQAIEVGLVRADERAVLFNCATGLK
                     YPLPDRSRTLDRHGPLDLSSL"
     gene            complement(3355..3837)
                     /locus_tag="VF589_00690"
     CDS             complement(3355..3837)
                     /locus_tag="VF589_00690"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEX8256119.1"
                     /translation="MPFLIRPLPHARFAPLFHLDEAELSARGAIRRTADAKPGFPCRV
                     SLADAEPGEEVILLHYEHHSAATPFRSGHAIYVRAAAVEADPAPGEVPAMLRSRMLSL
                     RGFDRHGMLKAADLADGAALEAPLEAMLAEPEVDYIHLHFAKAGCYAAKAVRTEPIRD
                     "
     gene            3961..5211
                     /locus_tag="VF589_00695"
     CDS             3961..5211
                     /locus_tag="VF589_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX8256120.1"
                     /translation="MTIRPGIFPTFFLSGFECSTFLWMDRGRRSLVEETRHREHADGD
                     YAMLRPLGIAVAREGIPWPMVDKGRGQYDFSYIDPFIAAMRRHDILPIWDLCHYGYPD
                     DLDPFSDEFTERFAAYAAAAAAYVTERHRGPYYFTPMNEITFFGYMGGEWGWCAPFQK
                     DHRCRLDFTAALARADIAAVKAIRDVMPSARMVHIDPLINVVAPVDRPDLEAAARHEA
                     EEDAFLAFDIIGGLKATEFGGSPEILDIVGFNNYSFGQMEYGGDAPNKPLDPGDPRIV
                     PLCDMMVRGWERYRRPMIIAETSGLEGGRDDWLNDVVRECLAAINRGVDIHAVCMFPA
                     VDMPDWHTGKWLNNGIADLVETPMGDLMRVPFQPYVEALHAWQRRLNRATGLDADPFD
                     SPVDLEDIVRAAREMQPEADPDWH"
     gene            complement(5316..5873)
                     /locus_tag="VF589_00700"
     CDS             complement(5316..5873)
                     /locus_tag="VF589_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX8256121.1"
                     /translation="MNRFRTAASAFCLAAAALVPTAAPAQQPAPAAAAFTDDPQAVLA
                     MKAGIWDADITFPNAEPGKQKAKGVQVNRLRSGGMWIINEFSVDGTPYEGTGIWGYDR
                     ASGRYSGIWTDTNDQKIRLDDGRWDPATRTMTWSADVAQPDGRHARYLFTEEFKGDTR
                     LFNMVALTRKGEVPLVSMVFTRRKG"
BASE COUNT          986 a         2059 c         2233 g          937 t
ORIGIN      
        1 agcgatgacc ctgccatggt tagcgggcgt gatcgaagtc gtcaccgggg cgctgatcgc
       61 gctcggcctg ttcacccgcc cggcggcgtt cctcgcctcg ggcacgatgg cggtggctta
      121 ttggatggcg cacgctccgc agggcccgtt tccggtcaac aatggcggcg acgcggcgat
      181 cctttactgc ttcgtcttcc tctacctcgc attcgtcggc gcgggtccgt ggagcctcga
      241 cgcgaagcgc aaccccgagg cgaatctggg caccggccac ggctccaacg gcgtgcagac
      301 ccactgacgg ccgaccggct gaaggcgaat ctgagaggtc ccccaaaacg aaacgccccg
      361 gcctaagccg gggcgccgtg gtggcagcga gcgggggggg ggggagcccg ctgcctgttg
      421 gggtaatgcg agaacgagcg tcgagcctgt tggttcccgg ataaaacgaa acgccccgac
      481 ataaggccag aggcgtctcg ccgacagcca gcgcgaagcg gctatctacc ccggacgaaa
      541 aacccatgag accggccggc gttccctcgg ccggcggacg gcccctgccg ggtcccctgc
      601 cccgccgcgc tcatgcgacg agccgaagca gctccgacac gggcacttcg agcaccggaa
      661 cgccgagctc gtcctcaacc tgaaggatgc gggcggaacc ggcgctcgcc gcctcatgct
      721 cggcggcgct acgccaggca tgataccagg cggcctcctg gtcgggcagc gtcatgccct
      781 cgcgatcctc gacgcggtcg gcgccctggc cgatgtggaa gtaataatgt ggcatgagga
      841 cagtaacgcc cggcgcaccc gcctgtttct cagaaaagaa ccctaaccgg cggtttactc
      901 tcatttattg ggacgggggc gcggcggcgc ttcgggccgc ttaatcgacg aaccgagccc
      961 ccgcgcggcc agcggaggaa cgggcgagtc taacctgggt tgtgttggaa gggatgcccc
     1021 cttcccactg aaaggattac ccgatgcagc gtcaaatagt atccgccgtg ttcgacaacc
     1081 gcgccgaagc cgagcgcgcc ttggcccagc tccgctccgc cggcgtgcgc gacgaggcga
     1141 tttcgctgat cgcccgccat gaaggccata gtggccacac cggcgacgat gacggcgatt
     1201 ccggcgacga tacccgcacc ggtctcggca tcggcctcgg cgcgggcgct ttgctcggct
     1261 tcggcgcatt gctgatcccg ggcgtcggac cgttcatcgc ggccggcgcg ctcgccgaga
     1321 ctctcggcgt caccggcggc gcgatcgcgg ccggcgcggc gatcggaggc gcagccggcg
     1381 gcctcgccgg agcgctcaag gaccacggcg tcaccgacga ggattcgacc tattatgagg
     1441 accggatcaa ccagggcggc gtgttcgtct cagtcgacac gtccaaggcc gatctcgacg
     1501 ccgagcgggc gcaggacatg ctctaccgcg ctggcggcca cagcgcgtcg cgcgcgcgca
     1561 cggcggacgc cctctaagtg ggccagggcc ggaggctcgc ccgagcctcc ggtccgccat
     1621 tcgatttcat gatccgcagc gtcaacgccc aattcgaaag ccgggccgaa gccgaggccg
     1681 ccgccgcgcg gctggagcgg tcgcacctcg cgccggagga cctccgcatc gacgtactga
     1741 attcggccgt accggccggc gccgagccgg gcctgttcga ccgcttcgcc gactggctgc
     1801 tgccgcggca gggccggccg cgccgcttca gggtctcggc caacgtcgat gagccgcagg
     1861 ccgacgaggc gatccgcatt ctacacgaag agccggtcca gccgatcatc ctaacgggcg
     1921 gcggcggatt tggaggcggg gtggtcgaga tggccgagac cgccgaggaa atcgtggtca
     1981 cgaagcaagc cgtcgtgcgc gaagaggtcg tgctcaccag ggaggccacg gaatgtgccg
     2041 agaccgtgca cgacacgctg cgccataccg aagtcgaggt gaaaagaatt gcgccccctc
     2101 ccccaccccg gcagtcctga cccgtgcgct gggacgacaa tctgaccgcc gaccggccga
     2161 gcttcgtcac ccatctcgaa tgttcgatga ccggcgagcg ctacgaggcc ggccggctgc
     2221 agggcctgtc gctggccgga cggccgctgc tggtgcgcta cgatctggcg cgggtgcgcg
     2281 aggcgctgac ccggcgcgcg ctggaggcgc ggccggcgga tttgtggcgc tggcgcgagc
     2341 tgttgccggt gcgcgcgaag gagaatatcg tcagcctggg cgaggccgcg acgccgctgg
     2401 tgcggctgga gcggaccggg gcggcgctcg gcgcgggcga attgctggtc aaggacgagg
     2461 gccggctgcc gaccggatcg ttcaaggcac gcggcctcgg cgtcgcggtg gcgatggcga
     2521 aggagctcgg cgtaaggcgg attgccatgc cgaccaacgg caatgccggc gccgccctcg
     2581 ccgcttatgc gagccgcgcc gggatcgaga cgatcgtgct ctgcccggac gacacgccgg
     2641 aagtgaacgt gcgcgagatc gcggcgcagg gcgcgcgggt ctaccgggtc aatggcctca
     2701 tcgatgattg cggcaggatc gtcgccgaag gcgcggccga gggccgctgg ttcgacatgt
     2761 cgacgctgaa ggagccctac cggatcgaag gcaagaagac gatggggctg gagctggccg
     2821 aacagctcgg ctgggcggtg ccggacgtga tcttctaccc gacgggcggc ggcaccggcc
     2881 tcatcggcat gtggaaggcc tttgccgagc tggaggcgat cggcctgatc ggcggcgagc
     2941 ggccgcggat ggtggcggtg caggccgagg gctgcgcgcc gatcgtcaag gcctggcagg
     3001 agggcgagga gcatgcgccg cgctgggaag atgcccatac ggtggcggcc ggcataaggg
     3061 tgccgcgcgc ggtcggcgac ttcctgatcc tgcgcgcggt gcgggagagc ggcggcttcg
     3121 ccatggcggt gtccgacgcc gcgctgaccc aggcggtgga ggatgcggcg cgcgacgacg
     3181 ggctgctgct ctgccccgag gccggggcga cgctcgcggc ctggcggcag gcgatcgagg
     3241 tcgggctggt gcgggcggac gagcgcgccg tgctgttcaa ctgcgccacc ggactcaaat
     3301 atccgctgcc cgaccgatcc cgcacactcg accggcacgg gccgctcgat ctctctagtc
     3361 tctgatcggc tcggtgcgaa ccgccttggc ggcgtagcag ccggctttgg cgaagtggag
     3421 atggatgtaa tccacttccg gctcggccag catcgcctcc agcggcgctt cgagcgcggc
     3481 gccgtcggcg aggtcggcgg ctttgagcat gccgtgccgg tcgaagccgc gcagcgacag
     3541 catgcgcgag cgcagcatcg ccgggacttc gcccggggcg ggatcggcct cgaccgcggc
     3601 ggcgcggacg tagattgcgt ggccggagcg gaacggggtc gcggcgctgt gatgctcgta
     3661 atgaaggagg atcacctcct cgccgggctc ggcgtcggcg aggctgaccc ggcagggaaa
     3721 gccgggcttc gcatcggcgg tgcggcggat cgcgccgcgg gcggaaagct ccgcctcgtc
     3781 gaggtggaag agcggcgcga aacgggcgtg tgggagcgga cggatcagga agggcatggg
     3841 catctccttt gcgccctcgc cttagggcgc ggcgcgcggc gcggcgtccc gatgcttgcg
     3901 ctcgaaaccg gcgcgaacgg agccccggag ccgttgccgc tcgcgaaacg tataagcggg
     3961 atgacgatca ggcccggcat cttcccaacc ttcttcctgt cgggcttcga atgctcgacc
     4021 ttcctgtgga tggacagggg ccgccgcagc ctggtcgagg agacccggca ccgcgagcat
     4081 gccgatggcg attatgccat gctgcgcccg ctcggcatcg cggtggcgcg cgaaggcata
     4141 ccctggccga tggtcgacaa gggccgcggc caatatgact tctcctatat cgacccgttc
     4201 atcgcggcga tgcgcaggca cgatatcctg ccgatctggg acctgtgcca ttacggctac
     4261 cccgacgacc tcgacccgtt ctcggacgaa ttcaccgagc gcttcgccgc ttatgccgcc
     4321 gccgccgccg cttatgtgac cgagcgccac cgcggcccct attacttcac gccgatgaac
     4381 gagatcacct tcttcggcta tatgggcggc gaatggggct ggtgcgcgcc gttccagaag
     4441 gaccatcgat gccggctcga cttcacggcg gcgctggcgc gggcggatat cgcggcggtg
     4501 aaggcgatac gcgacgtgat gccttcagcg cgaatggtcc acatcgaccc gctgatcaac
     4561 gtcgtcgcgc cggtcgaccg gcccgacctc gaggcggcgg cgcggcacga ggccgaggag
     4621 gacgcgttcc tcgccttcga catcatcggc gggctcaagg cgaccgagtt cggcggcagc
     4681 ccggaaatcc tcgatatcgt gggcttcaac aattacagct tcggccagat ggaatatggc
     4741 ggcgacgcgc ccaacaagcc gctcgacccc ggcgacccgc ggatcgtgcc gctgtgcgac
     4801 atgatggtgc gcggctggga gcgctaccgg cggccgatga tcatcgccga gacttcgggg
     4861 ctggagggcg gacgcgacga ctggctgaac gatgtcgtcc gcgaatgcct ggcggcgatc
     4921 aaccgcggcg tcgacatcca cgcggtctgc atgttcccgg ccgtcgacat gccagactgg
     4981 cacaccggca aatggctcaa caacggcatc gccgacctcg tcgaaacgcc gatgggcgac
     5041 ctcatgcggg tgccgtttca gccttatgtc gaggcgctgc acgcctggca gcgccggctc
     5101 aaccgcgcga ccgggctcga cgccgacccg ttcgacagcc cggtagacct cgaagacatc
     5161 gtccgcgcgg ctcgggagat gcagccggag gcggacccgg actggcatta aggcagcatc
     5221 gatccccggc gaaagccggg gtccagcttc gacttcctct gggcccccgc tttcgcgggg
     5281 gagcgatatc ggctgccgcc ccgagcgagc ggacgtcagc ccttgcggcg ggtgaagacc
     5341 atcgagacca gcggcacctc gcccttgcgg gtgagcgcga ccatgttgaa cagccttgtg
     5401 tcgcccttga attcctcggt gaagaggtag cgcgcgtggc ggccgtccgg ctgggcgacg
     5461 tcggccgacc aggtcatggt gcgcgtcgcc gggtcccagc ggccgtcgtc gaggcggatc
     5521 ttctggtcgt tagtgtcggt ccagatgccg gaatagcggc cgctcgcgcg gtcatagccc
     5581 cagatgccgg tgccttcgta aggcgtgccg tcgaccgaga attcgttgat gatccacatg
     5641 ccgcccgagc gcagccggtt gacctggacg cctttcgcct tctgcttgcc cggctcggca
     5701 ttggggaagg tgatgtcggc gtcccaaatg cccgccttca tcgcgagcac cgcctgcgga
     5761 tcgtcagtga atgcggcggc tgcgggcgcc ggctgctgcg ccggcgcggc cgtcggcacg
     5821 agcgcggcgg cggcaaggca aaaagccgag gccgcggtcc tgaacctgtt caatcttccc
     5881 tcccgttgcg gtgagcggcg cccagagcat ggcgggtgtc ggcaggcgcg gccgtttcga
     5941 cgaagctgga gaccggctcg acgagccggc gcgtcgcggc agcgcggttt cgactcgacc
     6001 ggacgggcgg gatatgagat aaatcgagcc gggtagaggc tttaggaggc agtcatggtc
     6061 atgtggaaga tcgcagctct gatcggcagc ggcatggtgc tggcgacgac ggcgtcggcg
     6121 caggagccga ccgggtccgg cgagggcttt tcctatccct atccgcggca atcgctgaag
     6181 atgcgcgaag agggcaccgt ccattaccgc gtccg
//
LOCUS       DASVRK010000016         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234 k141_2626,
            whole genome shotgun sequence.
ACCESSION   DASVRK010000016 DASVRK010000000
VERSION     DASVRK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2626"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VF589_00705"
     CDS             <1..608
                     /locus_tag="VF589_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX8256122.1"
                     /translation="PPAKVAILGGGVSGVNAAQMAVGMRADVTIYDINMERLAELDMF
                     FSSQIKTAYASKAAIASAVAKSELVIGAVLVPGAAAPKLVTRDMLKTMKRGSVLVDIA
                     IDQGGCFETSRPTTHENPVYEVDGIIHYCVANMPGAVARTSAFALNNATLPFVLKLAE
                     QGAEAAMRADPHLANGLNVSGGKIRHKAVAEALDLEFEPAG"
     gene            669..884
                     /locus_tag="VF589_00710"
     CDS             669..884
                     /locus_tag="VF589_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEX8256123.1"
                     /translation="MSPDQNQNPDPAAKDPDDWVTGDEAMTAPQASYLKTLAQEAGEE
                     FEPGLSKAEASKKIDELQHETGRGIDH"
     gene            complement(1026..1661)
                     /locus_tag="VF589_00715"
     CDS             complement(1026..1661)
                     /locus_tag="VF589_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8256124.1"
                     /translation="MGWLILLLLAAALFAALWRFARLEKGALQLLAAGLLLTMAGYAW
                     QGRPGLVGAPVPPPERQRLPDTAFAETRHAVLGQFDTADRWLTIADSYHRSGKTENAV
                     GVIRTAIRLYPRNADLWIGLGNALVVHGGGMSPAAELAFRRADEIAPNNPATGFFYGL
                     ALAQSGRIQEAELVWQAILPRIPAQVSWRPMVEERLAVIDQLRRMGALPPG"
     gene            complement(1661..2062)
                     /locus_tag="VF589_00720"
     CDS             complement(1661..2062)
                     /locus_tag="VF589_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX8256125.1"
                     /translation="MVAFLILVAVPAPAFADSSLPPADYADRQLPDPGQEARAKALME
                     ALRCLVCQGQSIADSDAEMAGDMRALVRRRIAAGEPPEAIRDWLVGRYGNWVSYDPPL
                     EPLTWPLWAAPIALLAAGLFLARGRFRRKRR"
     gene            complement(2356..2883)
                     /locus_tag="VF589_00725"
     CDS             complement(2356..2883)
                     /locus_tag="VF589_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX8256126.1"
                     /translation="MRRVVLWLPLAVFFVFALVVGLSLYAPTDGVIRSRMVGQPVPNF
                     ALPPAQPARRGLSSADLATGKPRLVNIFASWCLPCIAEAPQLMELRRRGVPIDAIAIR
                     DRPEDIAAFLSKWGDPYQRIGADPDSRVQMAFGSAGIPETFVVDGRGIIRHQHIGDVR
                     PEHVGEIVAAYEAAR"
     gene            complement(2880..>3067)
                     /locus_tag="VF589_00730"
     CDS             complement(2880..>3067)
                     /locus_tag="VF589_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX8256127.1"
                     /translation="GQLYTVIGKSDGQGRWQLRLWWKPAVTLIWLGGALVALGGLLSL
                     AGRVRRGRRARAEESYA"
BASE COUNT          541 a         1135 c          999 g          392 t
ORIGIN      
        1 tgccgccggc caaggtggcg atcctcggcg gcggagtgtc gggggtcaac gccgcccaga
       61 tggcggtcgg aatgcgggcc gacgtcacca tctacgacat caacatggaa cggctcgccg
      121 agctcgacat gttcttctca agccagatca agacggctta tgcctccaag gcggcgatcg
      181 ccagcgcggt tgccaagtcg gagctggtga tcggcgccgt gctcgtcccc ggcgcggccg
      241 cgccgaagct cgtcacccgc gacatgctga agaccatgaa gcgcggctcg gtgctggtcg
      301 acatagccat cgaccagggc ggctgcttcg agacctcgcg cccgaccacg cacgaaaatc
      361 cggtctacga agtcgacggc atcatccatt attgcgtcgc caacatgccc ggcgcggtcg
      421 cccgcacctc ggcctttgcg ctcaacaacg ccaccttgcc gttcgtcctg aagctggcgg
      481 agcagggcgc cgaagcggcg atgcgcgccg atccgcacct cgccaacggc ctcaacgtgt
      541 cgggcggcaa gatccgccac aaggcggtcg ccgaagcgct cgacctggag ttcgagccgg
      601 ccggctgaca cgggaacgaa ggaggaacat ggtcgttgaa ccggcgaatg cgccaagagg
      661 atacgaccat gtcccccgac cagaatcaaa accccgatcc cgccgccaag gaccccgacg
      721 actgggtcac cggcgacgag gcaatgaccg ccccgcaggc ctcttatctg aagaccctgg
      781 cgcaggaagc gggcgaggaa ttcgagcccg gtctcagcaa ggccgaagcc tcgaagaaga
      841 tcgacgagct ccagcacgag accggccgcg gcatcgacca ctaggcgcgg agcgggcgaa
      901 ggcgaatgaa cgtcacgccg gacttgatct aaccgtcacc ccggacttga tccggggtcc
      961 cgcttcctca tcgtgccgga aagaagcggg gtgccggatc aagtccggca tgacggcacc
     1021 gccccctacc ccggcggcag cgcgcccatc cgccgcagct ggtcgatgac ggcgagccgc
     1081 tcctccacca tgggtcgcca cgacacttgc gccgggatcc gcggcaggat cgcttgccac
     1141 acaagctcgg cctcctggat ccgcccgctc tgggcgagcg cgaggccgta gaagaagccg
     1201 gtcgccggat tgttcggggc aatctcgtcc gcccgccgga aggcgagctc ggccgccggg
     1261 ctcatgccgc cgccatgcac gaccagcgcg ttgccaagcc cgatccaaag gtcggcgttg
     1321 cgcggatata ggcggatggc agtgcggatc accccgactg cattctcggt cttgcccgac
     1381 cggtgatagc tgtcggcgat ggtgagccag cgatcggcgg tatcgaactg gccgaggacc
     1441 gcgtgacggg tctcggcaaa ggcggtgtcg ggcagccgct gccgctccgg cggcggcacc
     1501 ggcgcgccca ccagccccgg ccgcccctgc caggcatagc cggccatagt gagcagcagc
     1561 cccgcggcga ggagctgcaa cgcgcctttc tccagccgcg cgaaccgcca caaagccgcg
     1621 aacaaagccg ccgccagcag cagcaggatc agccacccca tcagcgccgc ttccggcgga
     1681 agcgcccgcg ggcgaggaac aaaccggcgg cgaggagcgc gatcggcgcc gcccagagcg
     1741 gccaggtcag cggctcgagc ggcggatcgt agctcaccca attgccgtag cggccgacca
     1801 gccagtcgcg gatcgcctcg ggcggctcgc ccgccgcgat ccgccgccgc acgagcgcgc
     1861 gcatgtcgcc ggccatctcg gcgtcgctgt cggcgatcga ctggccctgg cagaccaggc
     1921 agcgcagcgc ctccatcaac gccttcgccc gcgcctcctg ccccgggtcc ggcaactgcc
     1981 ggtcggcata atcggccggc ggcagcgagg aatccgcgaa ggcgggggcc ggcacagcca
     2041 ccaggatcaa gaaagccacc accccgtcat tcccgcgaaa gcgggaatcc cacttcttct
     2101 tgctagcccc gcggtcagcc gccgaaagaa aagcgggatt cccgcttccg cgggaatgac
     2161 ggagtgacag caggcccccg atgacgccgt tccccggcga aagccggggc ccagccacac
     2221 cgccctcctg ggccccggcc ttcgccgggg agctatcacg atgcaccccg ccctcgttcc
     2281 ccggcgaaag ccggggtcca gctacaccgt ccacctgggc cccggccttc gccggggaac
     2341 tggcaccgtg caccttcacc gtgccgcctc gtaagccgcg acgatctcgc ccacatgctc
     2401 gggcctcaca tcgccgatat gctggtggcg gatgatgccg cggccgtcga cgacgaaggt
     2461 ttccggtatg cccgccgacc cgaacgccat ctgcacccgg ctgtcggggt cggcgccgat
     2521 ccgctgatag gggtcgcccc atttcgaaag aaaggcggcg atgtcctcgg gccggtcgcg
     2581 gatcgcgatg gcgtcgatcg gcacgccgcg ccggcgcagc tccatcagct gcggtgcctc
     2641 ggcgatgcac ggcaggcacc agctcgcgaa gatgttgacc agccgcggct tgccggtggc
     2701 gaggtccgcg ctgctgagcc cgcgccgcgc cggctgcgcg gggggcagcg cgaaattcgg
     2761 caccggctgg ccgaccatgc gcgatcgtat cacaccgtcg gtcggggcgt agaggctcag
     2821 ccccaccacc aatgcgaaga cgaagaacac cgcgagcggc agccacagga cgacgcgcct
     2881 catgcgtagc tctcctcggc ccgcgcgcgg cgtccacgcc ggacccggcc tgccagcgac
     2941 aacagcccgc ccagcgccac cagcgccccg ccgagccaga tcaaggtcac cgccggcttc
     3001 caccacagcc gcaattgcca gcgaccctga ccgtcgctct tgccgatcac cgtatagagc
     3061 tggccgc
//
LOCUS       DASVRK010000017         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_252075, whole genome shotgun sequence.
ACCESSION   DASVRK010000017 DASVRK010000000
VERSION     DASVRK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252075"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VF589_00735"
     CDS             complement(<1..1750)
                     /locus_tag="VF589_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX8256128.1"
                     /translation="MTSAWTFAIDRGGTFTDIVARRPDGALVTRKLLSDNPEHYDDAA
                     VAGIRALMAEHGEAPIASVKMGTTVATNALLERKGEPVVLAITRGFGDALRIGYQARP
                     HIFARNIVLPEPLYGHVVEVHERVTAEGDILRPLDLDAAREQLRSAFHQGYRAIAIVL
                     MHGWRWTEHEAALAEAAGAIGFTQISASHQVGPLIRLVGRGDTSVVDAYLSPVLRRYV
                     DRVVAALGRETRLFFMQSSGGLTDAAAFQGKDAILSGPAGGIVGMVRTAAEAGFEKVI
                     GFDMGGTSTDVSHYAGTYERSTEKLVAGVRVRAPMLEIHTVAAGGGSVCHYDGARFRV
                     GPDSAGAVPGPACYRRGGPLTVTDCNVVLGKIRPDFFPAAFGPAGDQPIDAEASYARC
                     AEIAERAGLTLEETTQGFIRIAVENMGNAIKQISIARGHDVSRYTLQCFGGAGGQHAC
                     LVADALGMERVMIHPLAGILSAHGIGLADLTELRQRSLGGGNLDAALDELAGEAAAAL
                     QAQGAAEVQLHRRAALRYQGSDTALEVEVGPDMEAAFVEAHRQRFGFTSEAAVVVETA
                     IVEASSSPERGGGPPPQ"
     gene            complement(1888..3198)
                     /locus_tag="VF589_00740"
     CDS             complement(1888..3198)
                     /locus_tag="VF589_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8256129.1"
                     /translation="MRLPKSLAIAAAALLAAPAAAQAPAQNGITVIHAGTLLDRPGKA
                     PRRNATITVQAGKILSVQDGFAQPPAGAAVIDLRDRFVLPGLIDSHVHLSSDRAGNEG
                     LLAGLTESPQLGAYETLWNARKTLEAGFTTVRNLGDEGSTLALRDAIARGWAEGPRIV
                     DAASSISTTSGHMDARLGLREELHDAVPHENLCDGPEECRRAVRRQIGRGADVIKIAT
                     TGGVNSRIGAGLGAQMFEDEAKAIVETAHLYGKKVAVHAHGADGIAVALRAGADSIEH
                     GTLIDEESLRLFRKTGAYYVPTLSTVNGYLERIARDPNAYSPEVRAKIDWRIGITGQA
                     LRKAVPAGVKIAFGTDAGVSKHGRNADEFELMVKHGMTPTAAIHAATVNAADLLGLRD
                     QIGTLETGKAADLIAVAGDPLQDVTNLKRVGFVMKGGKVVKDAR"
     gene            complement(3216..>4236)
                     /locus_tag="VF589_00745"
     CDS             complement(3216..>4236)
                     /locus_tag="VF589_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX8256130.1"
                     /translation="TGRGSVGLASAAGYPIDIRLVFDRAQLARSDALGATVSGNVAVT
                     NNAARGPLISGDLQLPEVRYQIVRQGAAEVIELQGVRRKGAPLVRPDRVAQRTAAPSI
                     WKLDLRLRADNEVFVSGMGLESEWETDLRVGGTSNAPVVTGMARVIRGEISFAGNRFD
                     LTTGEVQFTGSRPIDPQLNIVASGDVEDVTINLNIGGNATDPEIAFTSSPALPQDELM
                     ARLLFGGPVTELNALQVVQLGASLNSLRGGGGGLNPLGRLRSATGLARLRFLGADEAT
                     GRGTAISAGFYISNKIYLEVITDARGFTATQLEIALSKALSILAQAGTSGGATNVNIR
                     YRKQY"
BASE COUNT          616 a         1315 c         1633 g          672 t
ORIGIN      
        1 cctgcggcgg cggtccccct ccccgttccg gggaggaact ggcctccacg atcgcagtct
       61 cgaccaccac cgccgcctcg gaagtgaagc cgaagcgttg gcggtgggct tccacgaagg
      121 ccgcttccat atccggcccc acctccactt cgagcgcggt atcgctgccc tggtagcgga
      181 gcgccgcgcg gcggtgcagc tgcacctcgg cggcgccttg ggcctggagg gcggcggcag
      241 cttcgcccgc cagttcgtcc agcgccgcgt cgagattgcc gccgccgagg ctgcgctggc
      301 ggagctcggt gagatcggcg aggccgatgc cgtgggcgga gaggatgccg gcgagcgggt
      361 ggatcatcac ccgttccatg ccgagcgcgt cggccacaag gcaggcatgc tgcccgcccg
      421 cgccgccgaa gcattgcagg gtgtagcggc tgacgtcatg gccgcgggcg atcgagatct
      481 gcttgatcgc gttgcccata ttctcgacgg cgatgcggat gaagccctgg gtcgtctcct
      541 cgagggtgag gccggcgcgc tccgcaatct ctgcgcagcg ggcgtaggag gcttcggcgt
      601 cgatcggctg gtcgccggcg gggccgaagg cggcggggaa gaaatcaggg cggattttgc
      661 cgagaacgac gttgcagtcg gtgacggtga gggggcctcc acgacggtag caagccgggc
      721 cggggacggc gccggcgctg tccggcccga cccggaagcg ggcgccgtca taatggcaga
      781 ccgagccgcc gccggccgcg acggtgtgga tctccagcat cggggcgcgg acgcgcacgc
      841 ctgcgaccag cttctccgtc gatcgctcat aggttccggc ataatgcgac acgtcggtcg
      901 aggtgccgcc catgtcgaag ccgatcacct tctcgaaccc ggcctccgca gcggtgcgga
      961 ccatgccgac gattccgccg gcggggccgg acaggatggc gtccttgccc tgaaaggcgg
     1021 ccgcgtcggt gagccccccc gaggattgca tgaagaacaa gcgggtctcg cggccgagcg
     1081 cggccacgac ccggtcgaca tagcgtcgga ggacggggga gagataggcg tcgaccaccg
     1141 acgtatcgcc gcggccgacg agcctgatca gcggcccgac ctggtggctg gcggatatct
     1201 gggtgaagcc gatcgcgccg gcggcctcgg cgagcgcggc ctcatgttcg gtccagcgcc
     1261 agccgtgcat caggacaatc gcgatggcgc gatagccctg gtgaaaggcg ctgcgaagct
     1321 gctcgcgggc ggcgtccagg tcgagcgggc ggaggatgtc gccctcggcg gtgacgcgct
     1381 cgtggacctc gacgacatgg ccgtagagcg gctcgggcag gacgatgttg cgggcgaaga
     1441 tgtgcgggcg cgcctggtag ccgatgcgaa gcgcgtcgcc gaagccgcgc gtgatggcga
     1501 ggacgaccgg ctcgcccttg cgctcgagga gggcgttggt ggcgacggtg gtccccatct
     1561 tcacggacgc gatcggcgcc tcgccatgct ccgccatcag cgcgcggatg ccggcgacgg
     1621 cggcgtcgtc atagtgttcg ggattgtcgg agagcaattt gcgggtgacg agggcgccgt
     1681 cgggtcggcg ggcgacgatg tcggtgaagg tgccgccgcg atcgatcgca aaggtccagg
     1741 cggaggtcat cggctaaagc cgttcgtgtc gagcgaagtc gagacatccc ttcgagctca
     1801 gccgagaaga agggcctcgg cttcgctcgg cagggtccct cgacttcgct cgggaccaac
     1861 gggcgggttt agcatattcc attccagcta ccgcgcgtcc ttgaccacct tgccgccttt
     1921 catgacgaag ccgacgcgct tcaggtttgt gacgtcctgg agcgggtcgc cggcgacggc
     1981 gatgaggtcg gcggccttgc cggtctcgag ggtaccgatc tggtcgcgca ggccgagcag
     2041 gtcggcggcg ttgacagtgg cggcgtggat agcggcggtg ggggtcatgc cgtgcttcac
     2101 catcaattcg aactcgtcgg cgttgcggcc gtgcttggag acgccggcgt cggtgccgaa
     2161 ggcgatcttg acgccggccg gaaccgcttt ccgcaaagcc tggcccgtga tgccgatgcg
     2221 ccagtcgatc ttggcgcgga cctcgggcga ataagcgttg ggatcgcgcg cgatccgctc
     2281 gagatagccg ttcacggtcg acagggtcgg gacgtaataa gcgcccgttt tgcggaacag
     2341 gcgcaggctc tcctcgtcga tgagggtgcc gtgctcgatc gaatcagcgc cggcgcggag
     2401 ggccacggcg atgccgtcgg cgccgtgggc gtggaccgcg accttcttgc cgtacaggtg
     2461 ggcggtctcg acgatcgcct tggcctcatc ctcgaacatc tgcgcgccga ggccggcgcc
     2521 gatgcggctg ttgacgccgc cggtggtggc gatcttgatg acgtcggcgc cgcggccgat
     2581 ctggcggcgg acggcgcggc ggcattcctc gggcccatcg cagagatttt cgtgcgggac
     2641 ggcgtcgtgc agctcttcgc gtaggcccaa gcgggcgtcc atatgccccg aagtggtcga
     2701 aatggacgag gcggcatcga cgatgcgcgg cccctcggcc cagcctcggg cgatcgcatc
     2761 gcgcagcgcg agagtcgagc cttcgtcgcc gaggttgcgg acggtggtga agccggcctc
     2821 gagcgtcttg cgggcgttcc acagggtttc ataggcgccg agctgcgggc tttcggtgag
     2881 accggcgagc aacccttcgt tgccggcccg gtcggaggag aggtggacgt ggctgtcgat
     2941 caggcccggc agcacgaagc ggtcgcgaag gtcgatgacg gcggcgccgg ccggaggctg
     3001 ggcgaagccg tcctgcaccg agaggatctt gccggcctgg acggtgatgg tggcgttgcg
     3061 gcgcggcgcc ttgccgggcc ggtcgagcag ggtgccagcg tggatgaccg tgataccgtt
     3121 ctgggcgggg gcctgggcgg cggccggggc ggcgagcagt gccgcggcgg cgatggcgag
     3181 cgattttgga aggcgcatat ttgtcctcga gacgatcaat attgtttgcg gtagcggatg
     3241 ttgacgttgg tagcaccgcc cgacgtgccc gcctgggcga ggatgctgag cgccttcgac
     3301 aaagcgattt cgagctgggt ggcggtgaag ccgcgggcgt cggtgatcac ttccaggtag
     3361 attttgttgg agatgtagaa gccggccgag atcgcggtgc cgcggccggt cgcctcgtcg
     3421 gcgccgagga agcggaggcg ggcgaggccg gtggcggagc gcaggcggcc gagtgggttc
     3481 aaaccgccgc cgccgccgcg cagcgagttc agcgaggcgc cgagctggac gacctggagc
     3541 gcattgagtt cggtgaccgg gccgccgaac agcagccgcg ccatcagctc gtcctgcggg
     3601 agagcgggcg aggaggtgaa ggcgatctcc ggatcggtgg cgttgccgcc aatgttgagg
     3661 ttgatggtga cgtcctcgac atcgccggag gcgacgatgt tgagctgcgg gtcgatcggc
     3721 cggctgccgg tgaactgcac ttcgccggtg gtgagatcga agcggttgcc ggcgaagctg
     3781 atctcgccgc gaatcacgcg cgccatgccg gtgacgaccg gcgcgttcga ggtgccgccg
     3841 acgcgaaggt cagtctccca ttccgattcg aggcccatgc cggagacgaa cacctcgttg
     3901 tcggcgcgca ggcgcaggtc gagcttccag atgctcggcg cggcggtgcg ctgggcgacg
     3961 cggtcggggc ggaccagcgg ggcgcccttg cggcggacgc cctgcagctc gatcacttcg
     4021 gcggcgccct ggcggacgat ctggtagcgg acctcgggca attgcaggtc gccgctgatc
     4081 agcgggccgc gggcggcatt gttggtgacg gcgacattgc cgctgacggt ggcgccgagc
     4141 gcgtcgctgc gggccagttg cgcgcggtcg aagacgaggc ggatatcgat cggatagccg
     4201 gccgccgatg cgaggccgac gctgccgcgc ccggtg
//
LOCUS       DASVRK010000018         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_562983, whole genome shotgun sequence.
ACCESSION   DASVRK010000018 DASVRK010000000
VERSION     DASVRK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562983"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2260
                     /locus_tag="VF589_00750"
     CDS             <1..2260
                     /locus_tag="VF589_00750"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HEX8256131.1"
                     /translation="GTGGTAGSGTGGSASLSVVAASFSGNSASLRADGTGGSGRAGAA
                     GGSGAGGTATLAVTGQGTTGGQLSLETFQANASGTGGAGSSTGPARYGEATILVRGGS
                     QASFGTIAMRTAGEAARPATEARPAPGPMAINVLDGTDNSDSLSFSADSIDVRSGGDL
                     NLFSADGGAIAASEKLLVAAGGNVAIRGVVTAPEIAIASADIDIANGARLGTAGVSAV
                     ALDAAGDGMTVIGGDEEGPGYTLTQAEAAAIEARSLTFTAARTGSDGGPDVLVRDLDI
                     RGSQDDGMESVSLVTSGHVRVEGAVEYRGAAATDQLSITAGERLEVITPTGSIRMTNA
                     DGRLSGLLQLLSQNIVVTDAALAARLADDPNFEGRDAALATNDGEVTPGGYVRAGGIS
                     LLTGKTLFVQNTGTATDFAGVTVGGEGLIIGRFQRSGGGGGGGGGGGEEGNFSFVGTL
                     ITKDDVLFFEFTIDSASQVTLRSYSYAGGTNAAGQVIQAGGFDPILSLFNASGALVGS
                     NDDGNEQVPVDPTTGNRYDVFFQETLQAGDYTVAVSAYPNFPNGDHVDDGFSNNGNLD
                     GRNPAFAFDVLGVDSSQGPGGDNGPIDVIAFGRRQNEDGSFTSGADFFNEVDFGRDEG
                     RVTYTDLSTFNGININDGVVEPPPPPPPPPPPPPPPPTPQPPPGIGTPDVVTGPLDNA
                     GDGPGGADGDGDGGPGSGFGSGFVGLIDTNSLSAEPLIEEPVASGGDTTMWNECSADE
                     ADGSDGNDRCTD"
     gene            2248..>3778
                     /locus_tag="VF589_00755"
     CDS             2248..>3778
                     /locus_tag="VF589_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8256132.1"
                     /translation="MHRLTLTAAAALLAAAPFATGALAQRVSLQDSFRIGSGAGVLCT
                     AQVASSSKALADMFDRAYSVVCRDAAVPVGQLYALKARGADPVQRLAALRLEKAECSA
                     AERQQVEGLGAVEVASCRMKELDVAYRVYQWRSRGTVYVAEGLAGYDSALRLGLRTLV
                     ADKPVAGEIAVATTEAGNPAAFARVQAGTLDPQRALAEAYRRNNAGNYAEAAEFFGSL
                     LGGEADETSQAEALVNQALQQSNLGNHAEANALFARARGLVAGDPVRARMYRNYRAMH
                     LLNQQRTEEALAELDRPLPRATSAAGAEIANLVIDPATAARLNAESPVTRQLASAGAG
                     GLLPDEKVQILEGQAQHLRGTILRRQGKAEQAAAALNAALSQLASVRGGRVTSTVWMR
                     GQLLGELGAIAEEGGNAAEAERQHLAAVALVGAYYPGSAALVNSQARLAAYYTRVGRA
                     EPALALYRRIVDANAEAASGSPALRRTLAPYFALLAERAADPAAVADMFKASQVLLRP
                     GV"
BASE COUNT          556 a         1406 c         1296 g          520 t
ORIGIN      
        1 cggcaccggc ggaacggccg gttccggcac cggcggctcg gcctcgctca gcgtcgtggc
       61 agccagcttt tccggcaaca gcgcttcgct tcgcgccgac gggaccggcg gctccggccg
      121 cgccggcgct gcgggaggct ccggggccgg cggtacggcc accctcgccg tcaccggcca
      181 gggcacgacc ggcggccagc tcagcctcga aaccttccag gccaatgcca gcggtaccgg
      241 cggggccggc agcagcaccg gcccggcgcg ctacggcgag gccaccatcc tggtgcgcgg
      301 cggcagccag gcgagcttcg gcacgatcgc gatgagaacc gcgggcgagg cggctcggcc
      361 ggcgaccgag gcccggccgg cgccggggcc gatggcgatc aacgtactcg acggcaccga
      421 caattcggac tcgctcagct tctcggccga ttcgatcgac gtcaggagcg gcggcgacct
      481 caatctgttc agcgccgacg gcggcgccat cgccgcctcc gaaaagcttc tcgtcgctgc
      541 cggcggcaac gtcgccatcc gcggcgtcgt gaccgcgccg gaaatcgcga tcgcgtccgc
      601 cgacatcgac atcgccaatg gcgcgaggct cggcaccgcc ggcgtctccg ccgtggcgct
      661 cgacgccgcg ggcgacggca tgacggtgat cggcggcgac gaggaagggc cgggctacac
      721 gctcacccag gccgaggccg ccgcgatcga ggcccgcagc ctcaccttca ccgccgcccg
      781 caccggcagc gacggcggcc cggacgtgct ggtccgcgac cttgatatcc gcggctcgca
      841 ggacgacggc atggaatcgg tgtcgctcgt caccagcggc catgtccgcg tcgaaggcgc
      901 cgtcgaatat cgcggcgccg cggccaccga ccagctcagc atcaccgccg gcgagcggct
      961 cgaggtgatc acgccgaccg gcagcatccg catgaccaac gccgacgggc ggctgagcgg
     1021 gttgctccag ctcctgtcgc agaacatcgt ggtcaccgac gcggccctcg ccgcgcgcct
     1081 cgccgacgac cccaatttcg aggggcggga cgcggcgctg gcgaccaatg acggcgaggt
     1141 aactccgggc ggctacgtcc gggcgggcgg catctcgctg ctgaccggca agaccctgtt
     1201 cgtccagaat accggcaccg cgaccgactt tgccggcgtc accgtcggcg gggagggcct
     1261 gatcatcggc cgcttccagc gcagcggcgg tggcggcggc ggcggcgggg gtggcggcga
     1321 ggaggggaat ttctccttcg tcggcacgct catcaccaag gacgacgtgc tgttcttcga
     1381 attcaccatc gattcggcct cgcaggtgac gctgcgcagc tattcctatg ccggcggcac
     1441 caatgccgct gggcaggtga tccaggccgg cggcttcgac ccgatcctgt cgctgttcaa
     1501 cgcctcgggc gcgctcgtcg gcagcaatga cgacggcaac gagcaggtcc cagtcgatcc
     1561 gacgaccggc aaccgctacg acgtcttctt ccaggagacg ctccaggcgg gcgattacac
     1621 ggtggccgtc tcggcctatc ccaatttccc gaacggcgat catgtcgacg acggcttctc
     1681 caacaatggc aacctcgacg ggcgcaatcc agccttcgcc ttcgacgtgc tgggggtgga
     1741 ttcctcccag gggccgggcg gcgacaacgg gccaatcgac gtgatcgcct tcggccgccg
     1801 ccagaatgag gatggcagct tcaccagcgg cgccgacttc ttcaacgaag tggatttcgg
     1861 ccgcgacgaa ggccgcgtca cctacaccga tctgtcgacc ttcaacggca tcaacatcaa
     1921 cgacggcgtg gtggagccgc cgccgccgcc gccgccgccg cctcctccgc ccccaccgcc
     1981 tccgactccc cagccgccgc ccggcatcgg cacgcccgat gtcgtgacgg ggccgctcga
     2041 caatgccggc gacgggcccg gtggtgccga cggcgacggt gacgggggac ctggctccgg
     2101 cttcggctcc ggtttcgtcg gcctcatcga caccaattcg ctgagcgccg agccgctgat
     2161 cgaggagccg gtggcgagcg gcggcgacac gacgatgtgg aacgaatgct ccgccgacga
     2221 ggcggacggc agcgacggga acgaccgatg cacagactga ccctcaccgc agccgcggct
     2281 ttgctggcgg cggcgccctt cgccaccggc gcgctcgccc agcgcgtgag cctgcaggac
     2341 agcttccgga tcggctccgg cgccggcgtg ctctgcaccg cccaggtcgc cagctcgagc
     2401 aaggcgctcg ccgacatgtt cgaccgcgct tattccgtgg tgtgccgcga cgccgcggtg
     2461 ccggtcgggc agctctatgc gctgaaggcg cgcggcgccg atcccgtcca gcggctggca
     2521 gcgctccggc tcgagaaggc cgagtgctcc gccgccgagc ggcagcaggt cgaaggcctg
     2581 ggcgcggtcg aggtggcgag ctgccgcatg aaggagctcg acgtcgccta tcgcgtctac
     2641 cagtggcgct cgcgcggcac cgtctatgtc gccgaagggc tggccggcta cgattcggcg
     2701 ctgcgcctcg gccttcgcac tttggtggcg gacaagccgg ttgcgggcga gatcgcggtc
     2761 gccaccaccg aagccggcaa ccccgccgcc ttcgcgcggg tccaggccgg cacgctcgat
     2821 ccgcagcgcg cgctggccga agcctatcgc cgcaacaatg ccggcaatta tgccgaagcg
     2881 gccgaattct ttggctcgct actcggcggc gaggccgacg agaccagcca ggccgaagcg
     2941 ctcgtcaatc aggcgctgca gcagtcgaat ctcggcaatc atgccgaggc gaacgcgctc
     3001 ttcgcccgtg cccgcggcct cgtcgccggc gatccggtgc gcgcccgcat gtatcgcaac
     3061 tatcgcgcca tgcacctcct caaccagcag cggaccgagg aagcgctcgc cgagctcgac
     3121 cggccgctgc cgcgcgccac ctcggccgcc ggggccgaga tcgcgaacct ggtgatcgat
     3181 cccgccaccg ccgcgcgatt gaacgccgaa tcgccggtca cccgccagct cgccagcgcc
     3241 ggcgccggcg gcctgcttcc cgacgagaag gtgcagatcc tcgaaggcca agcccagcat
     3301 ctgcgcggga cgatcctgcg ccgtcagggc aaggcggagc aggccgcggc cgcgctcaac
     3361 gccgcgcttt cgcagctggc ctccgtccgc ggcggccgcg tcacttcgac ggtctggatg
     3421 cgcggccagc tgctcggcga gctcggcgcc attgccgagg agggaggcaa tgccgccgag
     3481 gcggagcgcc agcacctcgc cgcggtagcg ctggtcggcg cttattatcc aggctcggcg
     3541 gcgctggtga acagccaggc gcggctcgcc gcttattata cccgcgtcgg ccgcgccgag
     3601 cccgctttgg cgctgtaccg ccggatcgtc gatgccaatg ccgaggcggc gagcggctcg
     3661 cccgccttgc gccgcaccct ggccccctat ttcgctttgc ttgccgagcg tgccgccgat
     3721 cccgccgcgg tcgccgacat gttcaaggcc agccaggtgc tgctccgccc cggcgtcg
//
LOCUS       DASVRK010000019         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_252344, whole genome shotgun sequence.
ACCESSION   DASVRK010000019 DASVRK010000000
VERSION     DASVRK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252344"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VF589_00760"
     CDS             <1..1496
                     /locus_tag="VF589_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8256133.1"
                     /translation="LQARLAAGNPRTNPYAAMPRGIGPALQDRSSSTAQTLDVDFVAN
                     GPVLELPAGEVSTTVRAGFQTGGFKSETIHAGIEQDRELARNGGNFQASLDIPIADRG
                     RGVLAAIGDLSANLNLEAEHLSDVGTLRTIGGGLNWSPVDEIDFIASYTDEEGAPTVE
                     QLGDPAVATPNFRVFDFITGETVEIIRIDGGNAALRPDRRRVLKLGATVKPFEKTGLS
                     LTANYVRSHIRDVIAAFPTATPEIEAAFPERFLRSESGRLIRIDSRPVNFARSDREEM
                     RWGLNFSKPLGPEPERPPGGWRPGGGAGGDEAARAARREARGSGRGRFGGGRFGGQGG
                     RIQLAFYHTWRIRDEVLIRDGVPKLDFLNGSAAGNTGGRPAHQLELQAGLFRNGLGAR
                     LTADWQSGTVVRGGPAGPGGASGDLRFSDLATVNLRLFADLGRQRALAERFPWLRGTR
                     VSLSVNNLFDARLGVRDATGATPLGYQPDYLDPLGRSVRITLRKLFF"
     gene            complement(1452..2030)
                     /locus_tag="VF589_00765"
     CDS             complement(1452..2030)
                     /locus_tag="VF589_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8256134.1"
                     /translation="MEQPEISAATEADLGALLALIEGAYRGDSARRGWTHEADLLGGQ
                     RTDEETLAEIVRDPQQAMLVARAGEALVGCVQLAWRGGGTAYLGMLSVDPSLQGGGLG
                     RRLIEAAEREAALRFGARGIEMTVIEQRAELIAYYVRRGYAVTGERRPFPLDDPRFGI
                     PRTRALAFVVLAKPIGPASEEQLAQRDPDGAA"
     gene            2258..>2885
                     /locus_tag="VF589_00770"
     CDS             2258..>2885
                     /locus_tag="VF589_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256135.1"
                     /translation="MAQRSRRRSGGAPALLLLLLCAVPAPAQSPETVEGLGPANGDWE
                     LEYVGQYGDADGSDAPRQHSGQSFYGVTDAFAIGGETLLSYRSGPLVEEDRIYFDYDS
                     VVGIFRFSDAERDPVGIGLWLQAGLDADGELARLEARLILEKKTLRWWLQANAMLRRV
                     NEEAEEGAHLAYAGRARFAALPDLWLGLEASGQAVRVSGFEREPFRESH"
BASE COUNT          414 a         1054 c          987 g          430 t
ORIGIN      
        1 ggctgcaggc gcgcctcgcc gccggcaacc cgcgcaccaa cccctatgcg gcgatgccgc
       61 gggggatcgg accggcgctc caggaccgct ccagctcgac cgcccagacg ctggacgtgg
      121 acttcgtcgc caacggtcca gtgctcgagc tgccggcggg agaggtctcg accaccgtgc
      181 gcgccggctt ccagaccggc ggcttcaaga gcgagacgat acacgccggc atcgagcagg
      241 atcgcgagct tgcccgcaac ggcggcaatt tccaggccag cctcgacatt ccgattgccg
      301 accgcggccg cggcgtgctg gcggcgatcg gcgatctttc ggccaatctc aacctcgagg
      361 cggagcatct ctccgatgtc ggcacgcttc gcaccatcgg cggcggcctc aactggtcgc
      421 cggtcgacga aatcgacttc atcgcctcct ataccgacga ggaaggcgct ccgacggtcg
      481 agcagctcgg cgacccggcg gtggcgacgc ccaatttccg ggtgttcgac ttcatcaccg
      541 gcgagaccgt cgagatcatc cgcatcgacg gcggcaatgc cgcgctgcgg cccgaccggc
      601 gccgcgtgct gaagctcggc gcgaccgtga agccgttcga gaagaccggc ctgtcgctca
      661 ccgccaatta tgtgcgcagc catatccgcg acgtcatcgc cgctttcccc accgccactc
      721 cggagatcga ggcggccttc cccgagcgct tcctcaggag cgagtccggg cgcctgatcc
      781 ggatcgacag ccggccggtc aatttcgccc gctcggatcg ggaggagatg cgctggggac
      841 tcaacttctc caagccgctc ggccccgagc cggagcggcc gccgggcggc tggcgtcccg
      901 gcggcggagc ggggggcgac gaggcggcgc gagcggcgcg gcgggaagcg cggggctccg
      961 gccgcgggcg cttcggcggc gggcgcttcg gcggccaggg cggccgcatc cagctcgctt
     1021 tctatcacac ctggcgtatc cgcgacgagg tgctgatccg cgacggcgtg ccgaagctcg
     1081 acttcctcaa tggttccgcc gccggcaaca ccggcggccg ccccgcgcac cagctcgagc
     1141 tccaggccgg cctgttccgc aacggcctcg gggcgcggct caccgccgac tggcagagcg
     1201 ggaccgtggt gcgcggcggc ccggccggcc ccggcggcgc gagtggagac cttcgcttct
     1261 ccgatctcgc caccgtcaac ctgcggctgt tcgccgacct cggccgtcag cgcgctttgg
     1321 ccgagcgctt cccctggctg cgcggcacgc gggtcagcct ctcggtcaac aatctcttcg
     1381 acgcgcggct cggcgtccgc gatgcgaccg gcgcgacccc gctcggctac cagcccgatt
     1441 atctcgaccc gctaggccgc tccgtccgga tcacgctgcg caagctgttc ttctgaagcg
     1501 gggccgatcg gcttggcgag cacgacgaag gcgagcgccc gcgtgcgcgg gatgccgaag
     1561 cgggggtcgt cgagcgggaa gggccgccgc tcgccggtga ccgcatagcc gcgccggaca
     1621 taataagcga tcagctccgc ccgctgctcg atcaccgtca tctcgatccc gcgggcgccg
     1681 aaccgaagcg ccgcctcgcg ctccgccgcc tcgatcagcc gccggccgag cccaccgccc
     1741 tgcaaggaag gatcgaccga cagcatgccg agataggcgg tgccgccgcc ccgccaggca
     1801 agctgcacgc agccgaccag ggcctcgccg gcacgcgcga ccagcatcgc ctgctgagga
     1861 tcccggacga tctcagccag cgtctcctcg tcggtgcgct ggccgccgag caggtccgct
     1921 tcatgggtcc agccgcggcg cgccgaatcg ccgcgatagg cgccttcgat cagcgccagc
     1981 agcgctccca gatcggcttc ggtggcggcg gagatttcgg gctgctccat gcgcgcgcct
     2041 atgccgcagc cgccttgccg gtgccagccg gtccccgctc cccggcggca aaatgcatcg
     2101 cccttaaccc tcttaagcgg cggcggacgc ggtccgcgct atgggcgagt cgcaccgcga
     2161 cgaggcgaga aatcatgacg gctgaacggt ttcgcatagg ctcgacctgg atcgccggcg
     2221 gcttcgtctg tgcgctgctg ctaatcgcca ccttctgatg gcgcagcgga gccgtcggcg
     2281 ctccggcggc gctccggccc tcctgttgct gctgctctgc gcggtgccgg cgccggcgca
     2341 gagccccgaa accgtggaag ggctcgggcc cgccaatggc gattgggagc ttgaatatgt
     2401 cggccagtat ggcgacgccg acggcagcga cgcgccgcgc cagcattccg gccaatcctt
     2461 ctacggcgtc accgacgcct tcgcgatcgg cggcgagact ttgctcagct atcgcagcgg
     2521 tccgctggtc gaggaggacc ggatctattt cgactatgac agcgtggtcg gcatcttccg
     2581 cttcagcgat gccgagcgcg atccggtcgg cattggccta tggctgcagg cggggctcga
     2641 cgccgacggc gagctggcgc ggctggaggc gcggctgatc ctcgagaaga agactctgcg
     2701 ctggtggttg caggccaacg ccatgctccg ccgggtcaac gaggaagccg aggaaggcgc
     2761 ccacctcgct tatgccggcc gagcgcggtt cgccgccttg cccgatctgt ggctcggcct
     2821 cgaagcgtcg ggtcaggcgg tgcgtgtcag cggcttcgag cgcgagccgt tccgcgaatc
     2881 tcatt
//
LOCUS       DASVRK010000020         7923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_501176, whole genome shotgun sequence.
ACCESSION   DASVRK010000020 DASVRK010000000
VERSION     DASVRK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501176"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VF589_00775"
     CDS             <1..288
                     /locus_tag="VF589_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8256136.1"
                     /translation="EENFAANLGIVDAVRAVADRHGVSLARIALAWLLAKGDDMVPIP
                     GVKRRFTLEDSMAAVDVALSAEDMAELDAAAPQGTTAGDRYSERGMRMVRI"
     gene            complement(285..866)
                     /locus_tag="VF589_00780"
     CDS             complement(285..866)
                     /locus_tag="VF589_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8256137.1"
                     /translation="MSKPAITTSRLLLRRWRSDDLEPFAAMCSDPEVMRYIGSGDTRN
                     RQQAAAAILAFERAWEESSYGLFAVELLGSNQLIGFTGLAEPAFLPEIMPAVEVGWRF
                     TRRSWGNGYATEAARAVPISVWPSWESLRSSAFIRSATLPPPGSCRSSECGSTEKLSI
                     RPAGAWSAYIEHAEGPLCVVSGPSAFHQTDPPF"
     gene            1071..2468
                     /locus_tag="VF589_00785"
     CDS             1071..2468
                     /locus_tag="VF589_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HEX8256138.1"
                     /translation="MHLRIAISTGALLLLAACGGGEGQPAAEAVRAQAAPVPTADTSW
                     AQPWAAAAPAPAQAVNTSPWVTGYYAGWFWDQMYPPERVDMSAMTHFVFGRVAPGGGS
                     LGGTAGTIVEGAGTAHHTGLSPDGRRSVEDYLIARAHRAGARALLMLGGDGADGLGFV
                     RSTADDIRPTFVRNIVDYLVRHRYDGVDVDWENELHGTPQLGVSAAEARRRLKALLVE
                     VRAEANRRLRFRAPNPPVLVTFPGYAVSINFLQPGGKVEQWQADVAHLVDQYNLMSYG
                     IGTAYNGGGWDSWFSGPIFGATGTTPYDLKTSIDAYVRTGVPRRKLGIGIGFYGIYYG
                     PGITGPRQPTEGNAVFEYSDAALGYSELVRKGYLSHGAYRWDAVAKATFRSYPNGGYV
                     PANDPGAAPAGFLSYEDERSIAAKGQWVRNAGLGGTILWTINYGYLPAKRTNPLLSAV
                     KASFLGAEAERRPLE"
     gene            complement(2475..3182)
                     /locus_tag="VF589_00790"
     CDS             complement(2475..3182)
                     /locus_tag="VF589_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX8256139.1"
                     /translation="MSALASLALATIAFVGTHFLLSHPLRAPLAARLGERGFAGLYLL
                     VAFVTLGWMILAWRSVENSDPLWIAPLWWWRIASGLMLLALILLVGSLVRNPALPHPG
                     AWPATIPPPRGVLAITRHPMNWSFMIWALVHLSLWGSPRNLIVAGGILILALFGSIGQ
                     DGKKLVLRGEAWRGWMEATSFVPFAALLSGRIPWRAAAPGWIAPVVGLILWLAITWYH
                     APLVSPIGGLGRLGGIG"
     gene            complement(3179..4585)
                     /locus_tag="VF589_00795"
     CDS             complement(3179..4585)
                     /locus_tag="VF589_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8256140.1"
                     /translation="MLKGAAGALLLTLMAGAGTAQTVAITGGTVALGDGSEPIPGGTV
                     VIRDGRIVAAGAGVAVPSGAQRVDATGKWVTPGLIAGFSRVGLAEVDAVDATNDVQAG
                     NSPFSAAIDVAPAINPRAAAISISRAAGVTRAVVAPATARSIFAGQGAVIDLGADMQP
                     ITRARAFQFVEFGETGADEAGGSRAATFVLFRNALREARDVSRFSPGIGGGGGGQREA
                     RASDQRDFPLEEVPDPNEASLRGNLSRPQDVLLTRFDAAALVPVLMGRQLLLVHVERA
                     SDILQVLGLRREFPRLRMVLVGATEGWTVAEEIRASGVPVIANALVDLPASFEQLAAT
                     QSNFGRMRAAGINVAMGTINEDETRQVRVSRQNAGNLVALTRVPGATGLSWGEALASI
                     TSRPAEVLGLGGELGSLQPGRRADVVIWDGDPLDTNSVAQMVMIDGVQQPLDNRQTKL
                     RDRYRSLEETTLPRAYNR"
     gene            complement(4591..5991)
                     /locus_tag="VF589_00800"
     CDS             complement(4591..5991)
                     /locus_tag="VF589_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8256141.1"
                     /translation="MAPKVLASVLAAALVTACADAGGAESASSKRPGTPYGRDPYPST
                     YRAYPGSPTLVTGVTIFDGEGARIDNGSVLFADGRIVEVGQQIAAPAGATVIDGQGKW
                     VTPGIIDIHSHLGDYPSPAVEAHSDGNEITNPVHPDVWAEHSVWPQDPGFSRALANGG
                     VTSLHVLPGSANLFGGRGVTLKNVQARTMQAMKFPGAPYSLKMACGENPKRVYGGRNQ
                     MPGSRMANVALMRQTWAKAAEYRRKWDKYERDGGEAPVREIGMDTLRGVLAGEITVQN
                     HCYRADEMAIIMDMAKEFGYKVAAFHHAAESYKIADLLRDNGVCSAMWADWWGFKMEA
                     YDGINENIPFVHNAGACAIVHSDDPNGIQRLYQEASKALAYGRRAGIQIPEEIAWTWL
                     SFNPAKALGIADRTGSLKPGKMADLVLWNGNPFSVYTRPERVWIDGALLYDANDPNRR
                     PVSDFELGQPGEGDVK"
     gene            complement(6011..7663)
                     /locus_tag="VF589_00805"
     CDS             complement(6011..7663)
                     /locus_tag="VF589_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX8256142.1"
                     /translation="MGFKPVSAWAEGDWIAATALLLLAGFLTAGALIAAGKKPEFAGH
                     PKGLYMLFFAEMWERFSFYGMRALLIFYLVQHWAFADDKSNLVYGSYLSLVYITPVLG
                     GYLADRYLGQRKAVVFGGLLLALGHGFMAFEGTGGQDDPTINVFWLALALIIVGSGFL
                     KANISVMVGQLYSRTDIRRDPAYTIFYMGINVGAALGTILVGYLGQTLGWGWGFGLAG
                     IGMLLGLVVFVLGKSVLNGAGEAPGPLSKQREYTLYAIGLGAVAVMWGLIQYQDVIQV
                     LLIISGLALLGYVLYESFKLPKEPRERMFAILFLIALNPLFWGLFEQAGGSMNLFTDR
                     FVDRGGVPASIFQSINPIYIILLAPLFAGLWQWLGTKGREPSAPAKFGLALLQMGLAN
                     LVLVWGAQAYGVAAMTPVLLVFMYYLLATTAELCLSPVGLSAMNRLAPSFLASLIMGA
                     WFYMTAVGNFLAGKIGEATGGHDGEMTKQGLLDIYAMFGWISIGVAVLVLLLSPIVKR
                     WMHLDTLRDREDNELAGYKELGENQGPGLFPQRETKPESKPL"
     gene            7776..>7923
                     /locus_tag="VF589_00810"
     CDS             7776..>7923
                     /locus_tag="VF589_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX8256143.1"
                     /translation="MFNDRSSLLSYLATRRSCRPRDLVEPGPDSDQLRRILEIAARSP
                     DHGKL"
BASE COUNT         1316 a         2741 c         2658 g         1208 t
ORIGIN      
        1 gaggagaatt tcgccgccaa tctcggaatc gtcgacgccg tccgcgcggt cgccgaccgc
       61 cacggcgtca gcctcgcccg gatcgcgctc gcctggctgc tcgccaaggg cgacgacatg
      121 gtgccgatcc ccggcgtcaa gcgccgcttc acgctcgagg acagcatggc cgccgtcgat
      181 gtggcgctga gcgccgagga catggccgaa ctcgacgccg ccgcccccca gggcaccacc
      241 gccggcgacc gctatagcga gcgcggcatg cggatggtgc ggatctagaa tggtgggtca
      301 gtttgatgaa aggccgaagg tccgcttacg acgcaaagcg gaccttcagc atgttcgata
      361 tacgcggacc aagcgcccgc aggtctgatc gataatttct ctgtcgaacc gcattccgag
      421 cttctgcatg atccgggcgg aggcagtgtt gccgacctga taaatgctga cgatctcaga
      481 gattcccagc tcggccaaac cgaaatcggc accgctcgtg cggcctcggt tgcatagccg
      541 ttgccccagc tccgacgtgt gaaccgccaa ccaacctcaa cggcaggcat gatctcggga
      601 aggaaggccg gctcggctaa gcctgtgaaa ccgatcagtt gattgctgcc cagcaactcg
      661 acggcgaaga gaccatagct actctcttcc caagctcgtt cgaacgcgag gatcgcggct
      721 gccgcctgct ggcgattacg ggtgtcaccc gaaccgatgt agcgcatcac ctcgggatcg
      781 ctgcacatgg ccgcgaacgg ctcgagatca tcgctccgcc agcgccttag cagcaagcgg
      841 cttgtggtga tcgcaggctt gctcatgcct tcagctatgt ccggacggac aaccatgtcc
      901 acaagcgtct gacgcgcccc gctcctgtac cgccggcgct tcgcaggaac agcttcgggt
      961 taccggcaca ttagccggaa ctcgtcgacg ggagcggcgg cttcatcgaa aatcgccctc
     1021 gcggcaggcg caatccgcca ccttcgccga aaccttgtcg gggggacctc atgcacctgc
     1081 gtatcgcgat ttcgaccggc gcgctgctcc tgctcgcggc ctgcggcggc ggcgaaggcc
     1141 agccggcggc cgaggcggtc cgggcgcagg cggccccggt gccgactgcc gacacgagct
     1201 gggcgcagcc ctgggcggcg gcggcccctg cccccgccca ggcggtcaac accagcccct
     1261 gggtgaccgg ctattatgcc ggctggttct gggaccagat gtacccgccc gagcgcgtcg
     1321 acatgagcgc gatgacccat ttcgtgttcg gccgcgtcgc ccccggcggc ggctcgctgg
     1381 gcggcacggc cggcacgatc gtcgaaggcg ccggcaccgc ccaccacacc ggcctctcgc
     1441 ccgacggccg gcgcagcgtc gaggattatc tgatcgcccg cgcccaccgc gccggcgcca
     1501 gggccttgct gatgctgggc ggcgacggcg ccgacgggct cggcttcgtc cgctccaccg
     1561 ccgacgacat ccgccccacc ttcgtccgca acatcgtcga ttacctcgtc cgccaccgct
     1621 atgacggcgt cgacgtcgac tgggagaatg agctgcacgg caccccgcag ctcggcgtca
     1681 gcgccgccga ggcgcgccgg cgcctcaagg ccttgctggt cgaggtccgc gccgaggcca
     1741 atcgccggct ccgcttccgc gcgcccaacc cgccggtcct ggtcaccttc cccggctatg
     1801 cggtcagcat caacttcctg cagcccggcg gcaaggtcga gcaatggcag gccgacgtcg
     1861 cccacctggt cgatcaatat aatctgatga gctacggcat tggcaccgct tataatggcg
     1921 gcggctggga cagctggttc agcggcccga tcttcggcgc gaccggcacc acgccctacg
     1981 acctcaagac cagcatcgac gcctatgtcc gcaccggcgt gccgcgccgc aagctcggca
     2041 tcggcatcgg cttttacggc atctattacg ggccgggcat caccggcccg cgccagccga
     2101 ccgagggcaa tgctgtcttc gaatatagcg acgccgcgct cggttattcg gagctggtcc
     2161 gcaaaggcta tctcagccac ggcgcctatc gctgggacgc cgtcgccaag gcgaccttcc
     2221 gcagctatcc gaacggcggc tacgtgcctg ccaacgaccc tggcgccgcg cccgccggct
     2281 tcctctccta tgaggacgaa aggtcgatcg cggccaaggg ccagtgggtg cgcaatgccg
     2341 gcctcggcgg cacgatcctg tggacgatca attacggcta cctgccggcc aagcggacca
     2401 acccgctgct gagcgcagtc aaggcgagct tccttggagc ggaggctgag cgccggccgc
     2461 tcgaatgagg aggctcagcc gattccgccg aggcgcccga gcccgccgat cggcgacacc
     2521 agaggcgcgt gataccaggt gatcgccagc cacaggatca gcccgacgac cggcgcaatc
     2581 caccccggcg ccgccgcgcg ccacgggatg cgtcccgaca gaagggcggc gaacggcacg
     2641 aacgaggtcg cctccatcca tccgcgccac gcctcaccgc gcaggaccag cttcttgccg
     2701 tcctggccga tcgatccgaa caacgccagg atcaggatgc cgccagcgac gatcaaattg
     2761 cgcgggctgc cccacagcga gaggtggacc agcgcccaga tcatgaagct ccagttcatc
     2821 gggtggcggg tgatcgcgag cacgccccgc ggcggcggga tggttgccgg ccacgccccg
     2881 ggatgcggca gcgcggggtt gcggaccagc gatccgacca gcaggatcag cgcgagcagc
     2941 atcaggccgg acgcgatccg ccaccaccag agcggcgcga tccacaacgg atcgctgttc
     3001 tcgacgctcc gccaggccag gatcatccag ccgagcgtga cgaacgcgac cagcaaatag
     3061 aggccggcga agccgcgctc gcccagcctt gccgccagcg gcgcgcgcag cgggtgcgac
     3121 agcaggaaat gggtgccaac gaaggcgatg gtcgccagcg cgagactggc gagcgcgctc
     3181 acctgttata ggccctcggc agggtggttt cctcgaggct gcgatagcgg tcgcgcagct
     3241 tggtctggcg attgtcgagc ggctgctgga cgccgtcgat catcaccatc tgcgccaccg
     3301 aattggtgtc gagcggatcg ccgtcccaga tcaccacgtc ggcgcggcgg cccggctgga
     3361 gcgagcccag ctcgccgccg aggccgagca cctcggccgg gcgcgacgtg atcgaggcga
     3421 gcgcttcgcc ccagctgagc ccggtcgcgc cgggcacccg ggtcagcgcc accaaattgc
     3481 cggcattctg ccgcgacacc cgcacctggc gagtctcgtc ctcgttgatc gtgcccatcg
     3541 cgacgttgat gccggccgcg cgcatgcggc cgaaattgga ctgggtcgcc gccagttgct
     3601 cgaacgaggc cggcaggtcg accagcgcgt tggcgatcac cggcacgccc gaggcgcgga
     3661 tctcctcggc cacggtccag ccttcggtcg cgccgaccag caccatgcgc agccgcggga
     3721 attcgcgcct gaggccgagc acctggagga tgtcgctcgc ccgctcgaca tggacgagga
     3781 ggagctggcg ccccatcagg accggcacca aagcggcggc atcgaagcgg gtcagcagca
     3841 cgtcctgcgg ccggctgagg ttgccgcgca gcgacgcctc attggggtcg ggcacttcct
     3901 cgagcgggaa gtcgcgctgg tcggaggcgc gcgcctcgcg ctgcccgccg ccgccgccgc
     3961 cgatgccggg cgagaagcgg ctgacgtcgc gcgcctcgcg cagcgcgttg cggaacagca
     4021 cgaaggtggc cgcgcggctg ccgcccgcct cgtcggcgcc ggtttcgccg aattcgacga
     4081 actggaaggc gcgggcgcgg gtgatcggct gcatgtcagc gccgaggtcg atcaccgccc
     4141 cctgccccgc gaagatgctc cgcgccgtcg ccggcgccac caccgcgcgg gtcacccccg
     4201 ccgcccggct gatcgagatc gcggccgcgc gcgggttgat cgccggcgcg acgtcgatcg
     4261 cggcgctgaa cggcgaattg cccgcctgca cgtcgttggt cgcgtcgacc gcgtcgacct
     4321 cggccagccc cactcgggag aagccggcga tcagcccggg cgtcacccat ttgccggtcg
     4381 cgtcgacccg ctgcgcgccg ctcggcacgg cgacgccggc cccggccgcg acgatgcgcc
     4441 cgtcgcggat gacgacggtg ccgccgggga tcggctccga tccgtcgccg agcgcgacgg
     4501 tgccgccggt gatggcgacg gtctgggcgg ttcccgcgcc cgccatcaaa gtcaggagga
     4561 gcgcgccggc cgcgcctttg agcatcgggc tcatttcaca tccccttcgc ccggctggcc
     4621 gagctcgaaa tcgctgaccg gccgccggtt cgggtcattg gcgtcgtaaa gcagcgcgcc
     4681 gtcgatccag acccgttccg gccgggtgta gacgctgaac ggattgccgt tccacagcac
     4741 caggtccgcc atcttgccgg gcttcaggct gccggtgcgg tcggcgatgc cgagcgcctt
     4801 ggcggggttg aagctgagcc aggtccaggc gatctcctcc gggatctgga tgccggcgcg
     4861 gcggccatag gcgagcgcct tggaggcttc ctggtagagc cgctggatgc cgttgggatc
     4921 gtccgaatgg acgatcgcac aggcgccggc attgtggacg aaggggatgt tctcgttgat
     4981 gccgtcatag gcttccatct tgaagcccca ccagtcggcc cacatcgccg agcagacgcc
     5041 gttgtcgcgg agaaggtcgg cgatcttgta gctttcggcg gcgtggtgga aggcggcgac
     5101 cttgtagccg aactccttgg ccatatccat gatgatggcc atttcgtcgg cgcggtagca
     5161 atggttctgg acggtgatct caccggccag cacgccgcgc agcgtgtcca tgccgatctc
     5221 gcggaccggc gcctcgccgc cgtcgcgctc atatttgtcc catttgcggc gatattcggc
     5281 ggccttggcc caagtctgcc gcatcaaggc gacgttggcc atgcggctgc cgggcatctg
     5341 gttgcgcccg ccatagacgc gcttgggatt ctcgccgcac gccatcttga gcgaataagg
     5401 cgcgccgggg aatttcatcg cctgcatcgt ccgcgcctgg acgttcttca aggtgacgcc
     5461 gcggccgccg aacagattgg ccgagcccgg cagcacgtgc aggctggtga cgccgccatt
     5521 ggcgagcgcg cggctgaagc ccggatcctg cggccacacg ctatgctcgg cccagacgtc
     5581 ggggtggacg ggattggtga tctcgttgcc gtccgaatgc gcttcgaccg cgggcgaggg
     5641 atagtcgccg aggtggctgt ggatatcgat gatgccgggc gtcacccact tgccctggcc
     5701 atcgatcacc gtcgctcccg ccggcgcggc gatctgctgg ccgacctcga cgatgcggcc
     5761 gtcggcgaac agcaccgagc cgttgtcgat gcgcgcgccc tcgccgtcga agatggtgac
     5821 tccggtgacc agggtcgggc tccccggata ggcgcggtag gtggagggat aggggtcgcg
     5881 cccgtagggc gtgccggggc gcttcgagga ggcgctttcc gcgcccccgg catcggcgca
     5941 ggccgttacg agagcggccg cgaggacgct cgccaggact ttcggtgcca tggatcgctt
     6001 tctgctggga tcagaggggt ttgctttcgg gcttggtttc gcgctgcggg aacaggccgg
     6061 ggccctggtt ctcgcccagt tccttatagc cggcgagctc attatcctcc cggtcccgca
     6121 gcgtgtcgag gtgcatccag cgcttcacga tcgggctcag caggagcacg aggacggcca
     6181 cgccgatcga gatccagccg aacatggcgt aaatgtcgag caggccctgc ttggtcatct
     6241 cgccgtcgtg gccgccggtc gcctcgccga tcttgccggc caggaaattg cccacggcgg
     6301 tcatgtagaa ccaggcaccc atgatcagcg acgccaggaa gctgggggcg aggcggttca
     6361 tcgccgacag gccgaccggc gacaggcaaa gctcggccgt ggtggcgagc agataataca
     6421 tgaagacaag aaggacgggc gtcatcgccg ccacgccgta agcctgcgcg ccccacacca
     6481 ggacgaggtt ggcgaggccc atttggagca gcgccaggcc gaacttggcc ggcgccgacg
     6541 gctcgcggcc tttcgtgccc agccactgcc agaggccggc aaagagcggc gccagcagga
     6601 tgatgtaaat cgggttgatc gactggaaga tcgaggccgg cacgccgccg cgatcgacga
     6661 agcgatcggt gaacaggttc atcgatccgc ccgcttgctc gaacaggccc cagaagagcg
     6721 gattgagcgc gatcaggaac aggatcgcga acatgcgctc gcgcggctcc ttgggcagct
     6781 tgaaactctc gtagaggaca tagccgagca gcgcgaggcc ggagatgatg agcagaacct
     6841 ggatcacgtc ctgatactgg atgaggcccc acatcaccgc gacggcgccg aggccgatcg
     6901 cgtagagcgt atattcgcgc tgtttggaga gcggccccgg cgcctcgccc gcgccgttga
     6961 ggacgctctt gccgagcacg aagacgacga ggccgagaag catgccgatg ccggccaggc
     7021 cgaagcccca gccccagccg agcgtctggc cgagatagcc gaccaggatg gtgccgagcg
     7081 ccgcgccgac gttgatgccc atgtagaaga tcgtataggc cggatcgcgg cggatgtcgg
     7141 tgcggctgta gagctggccg accatcaccg agatgttggc tttgaggaaa cccgagccga
     7201 cgatgatcag cgccagcgcc agccagaaga cgttgatcgt cgggtcgtcc tgcccgcccg
     7261 tgccttcgaa cgccatgaaa ccgtggccga gcgccagcag caggccgccg aacaccaccg
     7321 ccttgcgctg gccgagataa cggtcggcga gatagccgcc gagcaccggc gtgatgtaaa
     7381 cgagcgaaag gtaggagccg tagacgaggt tcgatttgtc gtcggcgaac gcccaatgct
     7441 ggacgaggta gaagatcagc agcgcccgca tgccgtagaa ggagaagcgc tcccacatct
     7501 cggcgaaaaa cagcatgtaa aggcccttgg gatggcccgc gaactccggc ttcttgccgg
     7561 cggcgatgag ggcgccggcc gtcaggaatc cggcgagcag gaggagggcg gtcgcggcga
     7621 tccagtcgcc ttctgcccaa gcggagacag gtttgaatcc cacggcgctt actcctcgcc
     7681 ctctggctgg cctaacaggg cgtgcaccct agcggccaaa actcatctgt gaagccgatt
     7741 gttgcactcc cttcaggctg ctagcgcggc ggccgatgtt caacgaccgc tcctccctgc
     7801 tctcctacct ggccacccgg cgctcctgtc gcccccgcga cctggtcgag cccggcccgg
     7861 actccgatca gcttcggcgg atcctggaga tcgccgcacg ctcgcccgac catggcaagc
     7921 tct
//
LOCUS       DASVRK010000021         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_252490, whole genome shotgun sequence.
ACCESSION   DASVRK010000021 DASVRK010000000
VERSION     DASVRK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252490"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1894)
                     /locus_tag="VF589_00815"
     CDS             complement(<1..>1894)
                     /locus_tag="VF589_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8256144.1"
                     /translation="NINPREAGVMDPQQRLVLEGAWRALEHAAIPPGSLRGTRTSVFL
                     GAADNEYLPLVRDVAALKADSAWLGTGNKVNVIAGRLSYILGLEGPSVTLDTACSSSS
                     VALHMAVQSLRSGESDLALAGGVNVILDPETFIGPCRAGMLSPTGRCRTFDRGADGYV
                     RAEGCGILVLKRVEDALRDHDRIVATIMGTAVNQDGRSSSLTAPSGPAQERVILAALD
                     DAGIDPGSIDYVEAHGTGTALGDPIEVNTLARVYGRTRGDRPPLLVGALKSVIGHSEA
                     AAGAGGVIKAALALESGTIPPNLHFEAPNPEFLEIEGLRFVTAPQPLDGEGPRHAAVS
                     AFGFSGTNAHIVLSAHSPPESSGPGDEGMPCWPVLFSARSEAALRAQLEQARQSLLRG
                     SPALGAISRGSSLGRDHFPMRIGFVVDSPDALSEAIEASLARPRLVPNFAEPPADAAA
                     MGENARLAAASAATPLDRWTRLVEAFEAGAIIDWAAAMADLPGPRAPLSVYPFERQRY
                     WPTATGPLVPGRTRSPTAGDYLLGDRVPTPRSPDIRYRRRFTPSSPAYVDDHRLLNTV
                     VVPAASHISMLLSAGRREFGRDECTASDIMLLAPLVIADDGEREVQLLLTPAEGGGFQ
                     AELVSAD"
BASE COUNT          287 a          640 c          705 g          262 t
ORIGIN      
        1 tgtccgcgct gaccagctcc gcctggaaac cgccgccctc cgccggagtc aacagcagct
       61 gcacttcgcg ttcgccgtcg tcggcgatga ccagcggggc gagaagcatg atgtcggacg
      121 cggtgcattc gtcgcggccg aactccctcc ggccagcgga gagcagcatc gagatgtgcg
      181 aggcggccgg cacgaccacc gtattcagca gccgatgatc gtcgacataa gcgggcgagc
      241 tcggcgtgaa gcggcgccga tagcgtatgt ccgggctgcg cggcgtcggc actcgatccc
      301 cgagcagata gtcgcccgcc gtcgggctgc gagtccggcc cgggacgagc gggccggtcg
      361 cggtcggcca gtagcgctgg cgttcgaacg gatagacgga gagcggcgcg cgcggccccg
      421 gcaggtcggc catcgcggcc gcccagtcga tgatcgcacc cgcttcgaaa gcctcgacga
      481 ggcgagtcca gcggtccagc ggagttgctg ccgaggccgc ggccagacgg gcattctcgc
      541 ccatcgcggc ggcatcggcg ggcggctcgg cgaaattggg gacgagccgg ggcctggcca
      601 aagaggcctc gatcgcctcg gacaaggcgt cgggcgagtc gacgacgaag ccgatccgca
      661 tcgggaaatg gtcgcggccg agactgctcc cgcgcgagat cgcgccgagg gccggggagc
      721 cgcgaagcag gctttggcgc gcctgctcca gctgagcccg caacgcggcc tcgctccggg
      781 ccgagaacag caccggccag catggcatgc cctcgtcgcc gggcccggac gattccggcg
      841 gggaatgggc cgagagcacg atatgcgcat tggtgccgga gaagccgaat gcgctcacgg
      901 cggcatgcct ggggccctcg ccgtcgagcg gctgcggcgc ggtgacgaac cgaaggcctt
      961 cgatttcgag gaattccggg ttgggcgctt cgaagtgaag gttcggcggt atcgttccgg
     1021 actccagcgc gagcgccgcc ttgatcactc cgcccgcgcc ggcggcggcc tcgctatggc
     1081 cgatcaccga tttcagggcg ccgaccagca gcggaggccg atcgccgcgg gtccggccgt
     1141 agacccgggc gagcgtgttg acctcgatcg ggtcgccaag cgcggtgccg gtgccgtgcg
     1201 cctcgacata gtcgatcgag ccggggtcga tcccggcatc gtccagcgcc gcaaggatca
     1261 cccgctcctg cgccggtccg ctcggcgccg tgaggctgct gctgcggccg tcctggttca
     1321 ccgccgtccc catgatcgtc gcgacgatgc ggtcgtggtc gcgaagcgcg tcctcgaccc
     1381 gcttgaggac gaggattccg cagccttcgg cccgcacgta gccgtccgcg ccgcggtcga
     1441 aggtgcggca gcggccggtg ggcgaaagca tgcccgcgcg gcaggggccg atgaacgtct
     1501 cgggatcgag gatgacgttg actccgccgg ccagcgccag gtcggattcc ccgcttcgca
     1561 ggctctgaac ggccatgtga agcgcgacgg aagaggaaga gcaggcggtg tcgagggtga
     1621 cgctcggtcc ctccaggccg aggatgtagg acaggcgccc ggcgatcacg ttgaccttgt
     1681 tgccggtgcc gagccaggcc gaatccgcct tcagggcggc gacgtcgcgg acgagcggga
     1741 gatactcgtt gtcggcggcg ccgaggaaga cgctggtccg ggtgccgcgc agcgagccgg
     1801 gcgggatcgc cgcatgctcg agcgcccgcc acgcgccctc gagaacgagg cgctgctgcg
     1861 gatccatcac cccggcctcg cgcgggttga tgtt
//
LOCUS       DASVRK010000022        13983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_563504, whole genome shotgun sequence.
ACCESSION   DASVRK010000022 DASVRK010000000
VERSION     DASVRK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13983
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563504"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VF589_00820"
     CDS             complement(<1..1284)
                     /locus_tag="VF589_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256145.1"
                     /translation="MADFNGTSGDDSYTGTQGYDRISGGGGDDHLDGAGGDDVIDGGS
                     GTNMLRGGDGSDTINSYGHHDSIEGGQGDNKINVYGVLGGTIDGGVTDDRGNPLIRSD
                     LFFHNASFDATSGTSVVRNFRTVSGRLLGTEGDDRVDLSSLRGGRGIEGFLTIVGGAG
                     RDSIVGSNLRDIMFGDAELPSWEGEVGEADVLRGRGGDDEIQGGVGDDDINGGNDQDI
                     LQGGSGADVVLGGGGNDIITGTRYFHHYRVEYADADAVADRLDGGEGWDRIYGGYGDI
                     IDGGTGASGESDILWLDLSEGNAGVTADFSSIAEGGTLAVGGGSITGIERLDELVGTR
                     FADDLTIFGGTTVRAGGGDDRVNGSEMDNNFTGEGGNDALYGHDGNDFLRGGVGNDVL
                     EGGSGADALGYGLSGIAVEAGDDVMNGGAGGDRLNG"
     gene            complement(1594..2700)
                     /locus_tag="VF589_00825"
     CDS             complement(1594..2700)
                     /locus_tag="VF589_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8256146.1"
                     /translation="MCNMGAALATMEAAAAPAAMDAAEQEFSARLLAMVNHATAGMMI
                     SIGHRTGLYEAMKAGGAMTSVELAAKAGLDERYVREWLGCMATAGIVALDATAGRYRL
                     PDAHGAFLGRDAANGNMAAMFQFIAVLGGVESRIVDCFRTGGGVPYQAYDRFHEVMAE
                     ESAQTVVAALEEAIVPLVPGLAAKLERGIAVADIGCGSGRALNRLAALYPNSRFTGYD
                     LCADAIAAAVREAREAGLANVAFAAADATDLEGEARFDLVLTFDAVHDQAHPAAVLAN
                     IRRLLKPGGVYLMQDIDSASAVADNVERPLAPFIYAISCMHCMTVSLAQGGDGLGAAW
                     GEELALAMLKNAGFGEVSTHRLEHDIMNLYYVCR"
     gene            2913..3065
                     /locus_tag="VF589_00830"
     CDS             2913..3065
                     /locus_tag="VF589_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256147.1"
                     /translation="MESLHEQRQRIRRTVFAAIAFACSVCALGGSVVAFAETGQPRSS
                     LARLPR"
     gene            3077..4663
                     /locus_tag="VF589_00835"
     CDS             3077..4663
                     /locus_tag="VF589_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8256148.1"
                     /translation="MQLPVPLPPSEKLKVRLIGELALVQGGAAVPLPASRKTRALFVY
                     LLQMRRPVRRERLCELFFDIPDDPRAALRWSLSKIRAMLGPFAGALVTCRERAGLSAD
                     AIITDVDQFRHWLGGAALPGEIHGYAEAALEPPLPGLDLNGLDSWNAWLAAERADIDE
                     VRAAFLARAAQDASLTPAHRHLFGLEAADLGAPPPLPVQPAARDADEAELAAKAANDP
                     IPPTGSAAPAEPMSIMGLDQHIHYCFGDDGVRIAYAATGDSSPPLVKAANWLNHLELD
                     WSGPVWGRLFHELSAHRRLVRYDERGNGLSDWDVEDLSFDAFVRDLEAVADHLGLERF
                     PLLGISQGCAVSIEYAARHPHRVSHLILLGGYAAGWRHLATPAEAEAREAAITLARHG
                     WGQDNPAYRQLFSHSFMPSATHEELDWFNDFQRRTATPANAARFLEAFSTIDVRHRLA
                     DIRCPTLVLHARGDQRVPIQQGIQLAASIPNASLVTLDSDNHIPLSREPATPRIVAAI
                     AAFLRAAPAAAPPNRRQLVA"
     gene            complement(4766..5569)
                     /locus_tag="VF589_00840"
     CDS             complement(4766..5569)
                     /locus_tag="VF589_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256149.1"
                     /translation="MLAGGVGDDLYLLEHSSPKVVEGSAQGHDSIHSSESATTLAANV
                     EDLIYFGSSEGSFVGTGNRGDNRIVAAAGSDTLKGAAGDDTLEGRAGKDALTGGDGDD
                     SLHGGAGHDLLTGGEGADRFVFDSALGAAHNVDRVADFTAGDFIVLDRNVFGAAGPQA
                     RLAGDAFVLGTAAADTEDRLITTRRPGGCSTMPTGRARGRRYCSRGWGRGPSSASPTS
                     RSSADGGLGRRLGTKPAAPVIPAQAGILRAGKKRGDPSFRWGDVSPSFR"
     gene            complement(6026..7690)
                     /locus_tag="VF589_00845"
     CDS             complement(6026..7690)
                     /locus_tag="VF589_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256150.1"
                     /translation="MAATEGDDRIIGTNGNDVIDGLGGNDHIDGGLGTDTISGGAGDD
                     VLFIEDRRGVAGESYSGGAGYDRLLIEPANLDLSLVLIGDDIETLDAIDGVRIAAGQA
                     AKFRAIDTHELIIGGTGIADLTDVQLVVQIITLADPGLELMLSEKDFFDAVVEGSAGT
                     DLVRGSETEDVVNGNGGNDIMYGNGGHDQLRGGEGADRLYGGDGSDWFLVYGSDNVAG
                     DIYDGGEGYDLILLAGDATLTRIVSIEEIESNYGVTLGARDFGALEEAEIASVTINKS
                     GVADLAGTELETDVINLAVPGVVLKLEGAIISTWTDQVAINGSLGVDRIIGTIGKDVI
                     STRDGNDVIDGGGGSGDILGGGAGDDSYRLRADNVIREVVDGGYDVVTTQLDAVTLAA
                     NVEKLVFDGPRTAAFVATGNATDNLIVARGGNDQLSGGGGADILHGGQGLDLLTGGAG
                     ADRFVFEAALSVGRNADRVMDFAAEDFILLDRGAFGAAGPQARLAGDAFVLGAAAKDA
                     EDRIVYDQASGRLFYDADGNGAGAQILFARLTAGTELGAADFQIVG"
     gene            8227..8379
                     /locus_tag="VF589_00850"
     CDS             8227..8379
                     /locus_tag="VF589_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256151.1"
                     /translation="MDDRTYYQRLEQGARYLAARAADEAERRIHLGWANRYFRLASES
                     PTRPAG"
     gene            8862..10649
                     /locus_tag="VF589_00855"
     CDS             8862..10649
                     /locus_tag="VF589_00855"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8256152.1"
                     /translation="MAKRSEDSTTYRSDELDQAALYGAETYNGDEHEDDEDDDDDHDD
                     DDDDDDCDDDSDDHDGEIDRVTGTNEADKLSFNITDDDPFRADLRGGLDTVKIRADDE
                     VDQVRIAFTQNEVGNGDPRDSDSQDNQDGGLAVRVRAEDEDGRLTGPISRFDDEGIRF
                     VAQGGLTFDVRELTDGSRIGDDFEVVVLGSSGDDFIDESDEHDDDDGYDDDDHDDDDH
                     DDDDSDDDDDHDEDCSCDDDDDGDKDDEDDGNVYAHGGRGDDVIIGGDGDDYLVGGAG
                     RDKLFGNDGRDTLIGGAGMDLLVGGDGHDTLFGGRGDDWLEGGDGNDRLDGGRGDNRI
                     DGGNGSDTVNFDISEDGSDRVNLGGGLDRVNVSGDDDVEEVRLTLTGGEVGDGSGRES
                     GDRDNQDGGLGVRLQAEDADGNVTGPVSRFDDEGIVFDGRDDGIRFDVRETATGNERG
                     TYDVVILGTDGDDRFDERGTTEDQYINGGRGSDELYGGGGQDFLAGGAGRDLLNGGRG
                     DDTLQGGNGDDVFQFRRRPGDDTILDFESGSDRIDLSAFDIDEDDVETETSGGDTVIF
                     VDTNDDGEANFNITLKNVDQVAAGDFMFG"
     gene            10934..11194
                     /locus_tag="VF589_00860"
     CDS             10934..11194
                     /locus_tag="VF589_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX8256153.1"
                     /translation="MLTYPVRLIPEGESSVMLIFPDVPEAVVVGADEQDAFDRAPALL
                     EAVLRGYVEEERPLPAPSDICGAPTVATPRFAKRARALAKAG"
     gene            complement(11709..13895)
                     /locus_tag="VF589_00865"
     CDS             complement(11709..13895)
                     /locus_tag="VF589_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256154.1"
                     /translation="MATEGNDIINGTTANDLIDGLGGDDVITGALGRDTISGGAGNDR
                     FVIRGQGDLAAGESYAGGAGWDELLISVNGVNISGLTIAADVESLRVGSADRLGDASL
                     RAGQFAAFRAVSAKELTITSAGAVDLRETELVTSSIKLGVAGVTLYLPEGPGGPARVT
                     GSSGRDIVTGTSAAETFEGSGGDDVLTGGGGNDLLTGGAGADVLNGGDGADKFDHAGA
                     AGGAGDRYYGGEGHDILYYSGIVSASGPATIARQIEELRLENLSSIDAGLLGAFSIVF
                     VEAVGDLTIATAGVCDLSGAAVTAGTIELGVGGITLKLTGVQKAQEVAGSDGTDVVVG
                     GSGADRLYGRDGDDRLSGGLGNDYIHAGLGLDIVNGGAGDDDLQIDARNLVAGEQYIG
                     GAGDDHVAVYGSTFLDVSAVTIAGVELISSSGALAMSAAQFGQFVNVTATQVAITTAG
                     YVKASAYLGTNSIRLEVGGSTLDMRSVDWADQYVLQGSGGDDVILGSNYSDVISGGDG
                     ADRLWGGSDGMDELRGGAGDDIYVDVVIIEGSLDEDVIVEGRDAGIDTVETRLTYGTL
                     PTNVENLIYTGAGIFYGGGNHDGNMIVGGARADRITAMGGDDRLHGGGGSDTLYGGAG
                     ADTFLFETLLGTGNVDKIGDFAAGDAIALDRAIFAALPGRGTALAGDAFVVGASAQDA
                     EDRIIYDQASGKLFYDADGNGAGARILFAQVTAGTEIGAADFQLFG"
BASE COUNT         2212 a         5223 c         4328 g         2220 t
ORIGIN      
        1 gccgttgagg cggtcgccgc cggcgccgcc gttcatgaca tcgtcgcccg cctcgacggc
       61 tatgccgctc aagccatagc cgagcgcgtc cgctccgctg ccgccttcca ggacatcgtt
      121 gccgacgccg ccgcgcagga agtcgttgcc gtcatggccg taaagcgcat cgttgccgcc
      181 ttcgcccgtg aaattattgt ccatctcgct gccgttcacg cggtcgtcgc cgcctcccgc
      241 gcggaccgtc gtgccgccga agatggtcag atcgtcggcg aagcgggtcc cgaccagctc
      301 atccagccgc tcgatgcccg tgatgctgcc cccgccgacg gccagcgtgc cgccctctgc
      361 gatcgaagag aaatcggccg tcacgcccgc atttccctcc gaaagatcga gccacaatat
      421 gtccgactcc ccggacgccc ctgtcccgcc gtcgataatg tccccgtagc cgccgtagat
      481 ccggtcccag ccctcgccgc cgtcgagcct gtccgccacc gcgtccgcgt ccgcatactc
      541 gacgcgataa tgatggaagt aacgcgttcc ggtgatgatg tcgttcccgc cccctccgag
      601 cacgacatcg gcgccgcttc cgccctggag aatgtcctgg tcgttgccgc cgttgatatc
      661 atcgtccccg acgccgccct ggatctcgtc gtcgccgccc cggcctctca ggacgtcggc
      721 ctcgcccacc tcgccttccc aggatggcaa ttcggcatcg ccgaacatga tgtcgcgcag
      781 attgctcccg acgatcgagt cgcggccggc gccgccgacg atggtgagga agccttcgat
      841 gccccgcccc cccctcaggc tgctgaggtc gaccctgtcg tcgccttcag tgccgaggag
      901 gcgcccggag acggtccgga aattgcgcac gaccgacgtt ccggaggtcg catcgaagct
      961 tgcgttatgg aagaacaggt cgctccggat cagaggattg ccgcgatcat cggtcacccc
     1021 gccatcgatc gtgccgccga gcacgccgta aacgttgatc ttgttgtcgc cctgcccgcc
     1081 ttcgatgctg tcatgatggc cgtagctgtt gatggtgtcc gagccgtcgc cgccgcgcag
     1141 catgttggtt cccgatccgc catcgatgac gtcgtcgccg ccggccccgt cgagatgatc
     1201 gtcgccgccg ccgccgctga tccggtcgta gccctgggtg ccggtatagc tgtcgtcgcc
     1261 gctggtgccg ttgaaatcgg ccatgtccgc atccccacct gttggtcgat ccggccatag
     1321 cctaaagccc ggcccgatga cagcggcagg cgccgcctga aatcaacagg ttgagagccg
     1381 gaagcggggg gcgcttccgg ctctcgccgg gtcgtcccgg caactcgcgc cagtagggca
     1441 agaccgccac gagaatggtt tctccgctgc taccgcagcg gagggaccgc ccaaggggcg
     1501 gtggagaggc ctcttaactc ctcccccgga tttccggggg agggggacca ggcgaagcct
     1561 ggtggagggg cgccggcgaa gccggccgga gcctcaccgg caaacataat ataaattcat
     1621 gatgtcatgc tccaaccgat gcgtgctcac ctcgccgaac cccgcattct tcagcatcgc
     1681 cagcgccagc tcctcgcccc acgccgcgcc cagcccgtcg ccgccctggg cgaggctcac
     1741 cgtcatgcaa tgcatgcacg aaatcgcgta gatgaacggc gccagcggcc gctcgacatt
     1801 gtccgccacc gcgctcgcgc tgtcgatgtc ctgcatcaga tagacgccgc ccggcttcaa
     1861 gagccgccgg atattggcca gcaccgccgc cggatgcgcc tggtcgtgga ccgcgtcgaa
     1921 cgtcaggacg aggtcgaacc gcgcctcgcc ctccagatcg gtggcgtcgg ccgcggcgaa
     1981 ggcgacgttg gccagcccgg cctcccgcgc ctcgcgcacc gccgccgcga tcgcgtcggc
     2041 gcacagatcg tagccggtga agcggctgtt cggatacagc gccgccagcc gattgagcgc
     2101 ccggcccgat ccgcagccga tatcggcgac cgcgatgccg cgctccagct tcgcggccag
     2161 ccccggcacc agcggcacga tcgcctcctc gagcgccgcc accaccgtct gtgcgctctc
     2221 ctcggccatt acttcgtgga accggtcata cgcctgatag ggcacgccgc cgccggtgcg
     2281 gaagcagtcg acgatccggc tctcgacccc gccgagcacc gcgatgaact ggaacatggc
     2341 ggccatgttg ccgttcgccg cgtcgcgccc gaggaatgcg ccatgcgcgt ccggcaggcg
     2401 gtagcgcccc gccgtcgcgt cgagcgcgac gatccccgcc gtcgccatgc agcccagcca
     2461 ttcgcgcaca tagcgctcgt cgagcccggc cttggcggcg agctccacgc tggtcatcgc
     2521 gccgcccgcc ttcatcgctt cgtaaagccc ggtgcggtgg ccgatcgaga tcatcatgcc
     2581 ggcggtggca tggttgacca tcgccagcag ccgcgcggaa aattcctgct cggccgcgtc
     2641 catcgcggcc ggcgcggcgg ccgcctccat cgtcgccagc gcggcgccca tattgcacat
     2701 gatcggctct ccttcggggg attggggttt cgttgatccc tggatagccg cgcgccgtgg
     2761 tccccaccgt ttgaacgagc gtggaaaagg cggcgccccc gtccgctgac acaattgaca
     2821 ccgaaaaccc ctccccacgg catcatcctg aaacccgcgc gcgcgaaaag ccgcatcact
     2881 gccggcgggt ccggcattgg ggagcctggg tcatggaaag ccttcacgag caaaggcagc
     2941 gcatccgccg caccgtcttc gccgccatcg cctttgcctg cagcgtctgc gcgctcggcg
     3001 gctcggtcgt cgccttcgcc gagaccggcc agccgcggag cagcctcgcg cggctgccgc
     3061 gctaggaggg gcggccgtgc agctgcccgt ccccctcccc ccttccgaaa aactgaaggt
     3121 ccgcctgatc ggcgagctcg ccctcgtcca gggcggcgcc gccgtgccgc tccccgcctc
     3181 ccgcaagacc cgcgccctgt tcgtctattt gctccagatg cgccgcccgg tccgccgcga
     3241 gcgcctgtgc gaattgttct tcgacatccc cgacgatccc cgcgccgccc tgcgctggtc
     3301 gctctccaag atccgcgcca tgctcgggcc gttcgccggc gccctcgtca cctgccgcga
     3361 gcgcgccggc ctcagcgccg acgccataat caccgacgtc gaccagttcc gccactggct
     3421 cggcggcgcc gctctccccg gcgagatcca cggttacgcc gaagccgcgc tcgaaccgcc
     3481 gctgcccggc ctcgacctca acggcctcga cagctggaac gcctggctcg ccgccgagcg
     3541 cgccgacatc gatgaggtcc gcgccgcctt cctcgcccgc gccgcccagg atgccagcct
     3601 gacccccgcc caccgccacc tgttcggcct cgaagccgcc gacctgggcg ccccgcctcc
     3661 gctccccgtg cagccggccg ccagggacgc cgacgaagcc gagctcgcgg cgaaagcggc
     3721 caacgacccc atccccccca ccggttcggc cgccccggcc gaaccgatgt ccatcatggg
     3781 cctcgaccag cacatccatt attgcttcgg cgacgacggc gtccgcatcg cctatgccgc
     3841 caccggcgac agctcgccgc ctttggtcaa ggccgccaac tggctcaacc atctcgagct
     3901 cgactggtcg ggcccggtct ggggccgcct gttccacgag ctttccgccc accgccgcct
     3961 cgtccgctac gacgagcgcg gcaacggact cagcgactgg gacgtggagg atctgagctt
     4021 cgacgccttc gtccgcgatc tggaagcggt cgccgaccat ctcggcctcg aacgctttcc
     4081 cctgctcggc atcagccagg gctgcgcggt ctcgatcgaa tatgccgccc gccatccgca
     4141 ccgggtcagc cacctcatcc tgctcggcgg ctatgcggcc ggctggcgcc atctcgccac
     4201 tccggccgag gccgaggcgc gcgaggccgc catcacgctg gcccgccacg gctggggcca
     4261 ggacaatcca gcctatcgcc aattgttcag ccacagcttc atgccgtccg ccacccatga
     4321 agagctcgac tggttcaacg atttccagcg ccgcaccgcc acgccggcca acgcggcgcg
     4381 cttcctcgaa gccttctcga ccatcgacgt ccgccaccgt ctcgccgaca tacgctgccc
     4441 gactttggtg ctccacgccc gcggcgacca gcgcgtgccg atccagcagg gcatccagct
     4501 tgccgcgtcc atccccaacg ccagcctggt gacgctcgac agcgacaacc acatcccgct
     4561 ctcgcgcgag cccgccacgc cccgcatcgt ggccgcgatc gccgccttcc tccgcgccgc
     4621 ccccgccgcc gccccgccaa accgccgcca gctggtcgcc tgaggggggg gtggggaacc
     4681 gggtcgccgg ggcatcgaat taaagccggg gaatcgtcat tccagcgaaa gccccgcgtc
     4741 attccagcga aagccccgcg tcatctcagc gaaagctggg ggaaacgtca ccccagcgaa
     4801 agctgggatc tcccctcttc ttcccggcgc gcaagattcc agcttgcgct ggaatgacgg
     4861 gcgcggcggg cttcgtgccc agcctccttc caagcccccc gtcagccgac gatctggaag
     4921 tcggcgacgc cgagctcggt ccccgccccc agccgcgcga acaatatctg cgcccccgcg
     4981 cccgtcccgt cggcatcgta gaacaaccgc ccggtcgcct ggtcgtaatc agccggtcct
     5041 cggtgtcggc ggcggcggtg cccagcacga acgcgtcccc ggccagccgc gcctgcggcc
     5101 cggccgcgcc gaacacgttg cgatcgagca cgatgaaatc acccgccgtg aaatccgcca
     5161 cgcggtcgac attgtgcgcg gcgccgagcg cgctgtcgaa cacgaagcgg tcggcgccct
     5221 cgccgcccgt caaaagatcg tggccggcgc ccccgtggag gctgtcgtcg ccgtcgccgc
     5281 cggtcagcgc atccttgccg gcgcggcctt cgagcgtatc gtcgcctgcg gcgcccttga
     5341 gcgtgtcgct tcccgcagcc gccacgatcc gattgtcgcc tcgattgccg gtgccgacga
     5401 agctgccctc gctcgagccg aagtagatca gatcctcgac gttggccgcg agggtcgtcg
     5461 cggactcgct gctatgtatc gagtcatgac cctgcgccga cccttcgaca accttgggcg
     5521 atgagtgttc gaggagatag agatcgtcgc cgacgccgcc cgccagcacg tcgccgctgc
     5581 tgttcccccg tccaggacat cgtccccgcc atagccgtag atcgtgtcgg cttcttcgcc
     5641 gccggtcaca atgtctttgc cggccgagcc catgaccacg aagccctggt agctatagcc
     5701 gccgagatcg agggtgatgc cgccgaccgc cagcctgaag gcgttcgtgc ctatccctat
     5761 atcgggccgg aattcgccgg cattggcgat gatgagggta tcattccagc gaaagctgga
     5821 gccagagacg accgcaggtc aacctgactt cttcttcctt cgcgcagaat aagcgggatt
     5881 ccaggttcgg gccaaaccgt cattccagcg aaagctggaa tctcccttct tcttcccgcg
     5941 tctcccaaga agtgagatcc cagcttccgc tgggatgacg cgggtgcgac gcggctgcga
     6001 cgacgcgcct cccccagccc cccggtcagc cgacgatctg gaaatcggcg gcgccgagct
     6061 cggtccccgc ggtcagccgc gcgaacaaga tctgcgcccc cgcgccattg ccgtcggcgt
     6121 cgtagaacag ccgcccgctc gcctggtcgt agacgatccg gtcctcggca tccttggccg
     6181 cggcgccgag cacgaacgcg tcgccggcca gccgcgcctg cggcccggcc gcgccgaacg
     6241 cgccgcggtc gagcaggatg aaatcctcgg ccgcgaaatc catcacccgg tcggcgttgc
     6301 gcccgacgct gagcgcggcc tcgaacacga accggtcggc gccggcgccg ccggtcagca
     6361 ggtccagccc ttggccgccg tggaggatgt cggccccgcc gccgccgctc aactggtcgt
     6421 tgccgccgcg cgccacgatc agattgtcgg tcgcgttgcc ggtggccacg aacgccgccg
     6481 tcctggggcc gtcgaacacc agcttctcga cattggcggc gagcgtcacc gcgtcgagct
     6541 gggtggtcac gacgtcgtag ccgccgtcca ccacctcgcg gatgacgttg tccgcgcgca
     6601 gccggtagct gtcgtcgccg gcgccgccgc ccaatatgtc gccgctgccg ccgccgccgt
     6661 cgatcacgtc gttgccgtcg cgggtggaaa tgacgtcctt gccgatcgtg ccaatgatcc
     6721 tgtcgacgcc gaggctgccg ttgatcgcga cctggtcggt ccatgtcgag atgatcgccc
     6781 cctccagctt gagaaccacg ccgggcacgg cgagattgat cacgtcggtt tcgagctcgg
     6841 tgccggccag atcggcgacg ccggacttgt tgatcgtcac gctggcgatc tcggcctctt
     6901 ccagcgcgcc gaaatccctc gccccgagcg tcacgccata gttgctttca atctcttcga
     6961 tgctgacgat gcgcgtgagc gtcgcgtcgc ccgccaacag gatcagatca tacccctcgc
     7021 cgccgtcata gatgtcgccg gcgacattgt ccgacccgta gaccaggaac cagtccgagc
     7081 cgtcgccgcc atagaggcgg tccgcgccct cgccgccgcg cagctgatcg tggccgccat
     7141 tgccgtacat tatgtcgttg ccgccattgc cgttgacgac atcttcggtt tcggagccgc
     7201 ggacgaggtc ggtgccggcg ctgccctcga ccacggcatc gaaaaagtcc ttttccgaaa
     7261 gcatcagctc gagcccggga tcggcgaggg tgatgatctg caccaccagc tggacgtcgg
     7321 tgaggtcggc gatgccggtc cctccgatga tcagctcatg ggtgtcgatg gccctgaatt
     7381 tggccgcctg gccggccgcg atgcggaccc cgtcgatcgc gtccagcgtc tcgatgtcgt
     7441 cgccgatcag caccaggctg agatcgaggt tggcgggctc gatcagcagc cggtcgtacc
     7501 ccgcgccgcc ggaatagctt tcgcccgcga ccccgcggcg gtcctcgata aacagcacgt
     7561 cgtcgccggc gccgccgctg atcgtgtcgg tgccgaggcc gccgtcgata tggtcgttgc
     7621 cgccgagccc gtcgatgaca tcgttgccgt tcgtcccgat gatccggtcg tcgccttcag
     7681 tcgccgccat ttggtccccc acgtctcact atgcggcaag gtggcgacgc gcgtccgccg
     7741 ccgcccgcta cgtgcgtgcc gcgccgcgcc gcgtctttgg aaaaccacga atccccccgc
     7801 ccccgaacct tagcagccgc cgctcccacg gcaagcgccg cgtcattgca gcggaaagcg
     7861 ggagccgggg gcaccgctga aagcccttcg tcattccagc gaaagctgaa ctctattttc
     7921 actcgggtgc tgaaagaagt gagatcgcag cttccgctgg gatgacgccg gaccaccgcc
     7981 cccccccccg ctcaatagcc gataaaatcc cgtgcccgag caccgtcccg gcctcgaccc
     8041 cagcgaacaa aatcgcctcg cccgcgccgc tgccgtcggc atcgtagaag atccgcccgg
     8101 tctcgctttg gtagaggatg cggtcgtccg cctcgggccg gaacgccgcc ccttccgggc
     8161 cggcttgatt gcgcccccgg aactttccgg cccccggagc gctgagcccc ccgcagggag
     8221 gctcgtttgg acgatcgcac gtattaccag cggctcgagc agggcgcccg ctatctcgcc
     8281 gcccgcgccg ccgacgaggc cgagcgccgc atccatctcg gctgggccaa ccgctacttc
     8341 cgcctcgcct ccgaatcccc cacccgcccc gccggctgag ggtcgcgcgg cactcgtgcc
     8401 cctgcgaagg caggggccca gaccaggcgc agcctgtcgg gcgagggccg gctacagtcg
     8461 cggcagtcgc ggttcatctg ggcggagaaa agtctggacc cctgccttcg caggggaaca
     8521 ggtgcggcgc tcgcggccct gccttcgcag gggaacaggt gcggcgaccc tattcgctca
     8581 atcctgagga gggactgacc ccggacttga tccgggggaa tgcgactcga aggaccaccc
     8641 caacgccaca tccccaccaa accacgactc atcgctacgc atgagaaaca agtctatcct
     8701 aacccgatta cttgactaat ccatattaac catgcgcatc caaagtagat gatgctggtt
     8761 ttagcggcac tcgcgaagac gtgcccccgc gccttcgaag ggatcatcgc cagcgcggct
     8821 tatcgctcca ccggccttca tcgctatcag ggaagattgt catggcaaag cgttcggaag
     8881 actccaccac ctatcgcagc gacgaactcg accaggccgc tctttacggc gccgaaactt
     8941 acaacggcga cgagcacgaa gacgacgagg atgatgacga cgatcatgac gacgacgatg
     9001 acgacgacga ttgcgacgac gacagcgacg accatgacgg cgaaatcgac cgcgtgaccg
     9061 gcaccaacga ggccgacaag ctctccttta acatcaccga cgacgatcct ttccgcgccg
     9121 accttcgcgg cggcctcgac acggtcaaga tccgggccga cgacgaagta gaccaggtcc
     9181 gcatcgcctt cacccagaat gaagtcggca acggcgaccc gcgtgacagc gacagccagg
     9241 acaaccagga tggcggcctc gccgtccgcg tccgcgccga ggatgaggac ggccgcctga
     9301 ccggcccgat tagccgcttc gacgacgaag gcatccgctt cgtcgcccag ggcggcctca
     9361 ccttcgacgt ccgcgagctc accgacggca gccgcatcgg cgacgatttc gaagtcgtgg
     9421 tgctcggcag cagcggcgac gatttcatcg acgagagcga cgagcatgac gacgacgacg
     9481 gctatgacga cgacgatcat gacgacgacg atcatgacga cgacgacagc gacgacgacg
     9541 acgatcatga cgaggattgc agctgcgacg acgatgacga cggcgacaag gacgatgagg
     9601 atgacggcaa cgtctacgcc catggcgggc gcggcgacga cgtcatcatc ggcggcgacg
     9661 gcgacgatta tctggtcggc ggcgccggcc gcgacaaatt gttcggcaat gacggccgcg
     9721 acacgctgat cggcggcgcc ggcatggacc tgctggtcgg cggcgacggc catgacacgc
     9781 tcttcggcgg ccgcggcgac gactggctcg aaggcggcga cggcaacgac cggctcgacg
     9841 gcggccgcgg cgacaaccgc atcgacggcg gcaacggcag cgacacggtc aatttcgaca
     9901 tctccgagga cggctccgac cgggtcaatc tcggcggcgg cctcgaccgg gtgaacgtgt
     9961 ccggcgacga cgatgtcgag gaggtccgcc tcaccctcac cggcggcgag gtcggcgacg
    10021 gctcagggcg cgagagcggc gaccgcgaca accaggatgg cgggctcggc gtccggctcc
    10081 aggccgagga cgccgacggc aatgtcaccg gcccggtcag ccgcttcgac gatgaaggca
    10141 tcgtcttcga cggccgcgac gacggcatcc gcttcgacgt ccgcgagacc gcgaccggca
    10201 acgagcgcgg cacctacgac gtggtcatcc tcggcaccga tggcgatgac cgcttcgacg
    10261 agcgcggcac caccgaggat cagtacatca acggcggccg cggctccgac gagctctacg
    10321 gcggcggcgg gcaggatttc ctggccggcg gcgcgggccg cgacctcttg aacggcggcc
    10381 gcggcgacga cacgctccag ggcggcaatg gcgacgacgt cttccagttc cgccggcgtc
    10441 cgggcgacga taccatcctc gacttcgaat ccggctccga ccgcatcgat ttgagtgcgt
    10501 tcgacatcga cgaggacgat gtcgagaccg agacctcggg cggcgacacg gtgatcttcg
    10561 tcgacaccaa cgatgacggc gaggccaatt tcaacatcac cctcaagaac gtcgaccagg
    10621 tcgccgccgg cgacttcatg ttcggctgat gacaggcgcc gccgctccct tggggggcgg
    10681 cggcggccca aatgccagcg caaccaaccc ggcattccgg cgcaagctgg aatctccctt
    10741 cctccttcag tgcggaaggc aagtgagatc ccagctttcg ctgggatgac gtggcggctt
    10801 gcgctggaat gacgcctgcg cacccctcct ccgcgccggc ggcttcgtct tttcaactta
    10861 gcctcgcccc cgggcggaac tccgcccgcg cttaagcgct tcgtttgctt gcactaagcc
    10921 cccggagccg ccgatgctga cctatcccgt ccgcctgatc cccgaggggg aatcgtcggt
    10981 catgctgatc ttccccgacg ttcccgaagc ggtcgtcgtc ggcgccgacg agcaggacgc
    11041 cttcgaccgc gcgcccgccc tactcgaggc ggtgctgcgc ggctatgtcg aggaggagcg
    11101 cccgctcccc gccccctccg acatatgcgg cgcccccacc gtcgccaccc cccgcttcgc
    11161 caaacgcgcc cgcgccctcg ccaaggcggg ctgaggcggc ggcggccggt tcaggcggtc
    11221 agtcgaacag ctgcaaaagc accggcattc accaatccgc tgaccgtgcc cctgcgaagg
    11281 caggggccca gaccaggcgc agcctgtcgg acgagggtag aggtcgcgcc agtccccgtc
    11341 atgccggact tgatccggca tccgccttcc cggccgatcg ctcagtcgcc gccaaagccg
    11401 attggtcggc ggctccgctt gcttgccatt gtcctccggg atgccgtgcc gcaggttcca
    11461 gatgacagcc gcgatcagca tgaagccaag cgcagccccg accatctcca ccacgctcat
    11521 tccggcttct ccaccggctt aggctccatc atggatctcc catgctggag aggccgatat
    11581 aggcggccaa cactgacagt agcacgaagg cgagcacgac gccgccgaga atcttccagt
    11641 tcactccggc ttccgggcgg gttcatgccc gacccgcgcc gcgcgccggt tcaggcggtc
    11701 cagccgcctc agccgaacag ctggaaatca gccgcgccga tctccgtccc cgcggtcacc
    11761 tgcgcgaaca atatcctggc gccggcgccg ttgccgtcgg cgtcgtagaa cagcttgccg
    11821 ctggcctggt cgtagatgat ccggtcctcg gcgtcctgcg cgctggcccc gaccacgaag
    11881 gcatcgccgg ccagcgccgt gccgcgcccc ggcaacgcgg cgaaaatcgc gcggtcgagc
    11941 gcaatcgcat ccccggccgc gaaatccccg atcttgtcga cattgcccgt gcccagcagg
    12001 gtttcgaaca ggaaggtgtc ggcgcccgct ccgccgtaca gcgtgtcgct gccgccgccg
    12061 ccgtgcaggc ggtcgtcccc gcccatggcg gtgatccggt ccgctctggc cccgccgacg
    12121 atcatattgc cgtcgtggtt gccgccgccg tagaaaattc cggcgccggt gtagatcaga
    12181 ttctccacgt tggtgggcag cgtgccgtaa gtgagcctgg tctcgaccgt gtcgatgccg
    12241 gcgtcccgtc cctccacgat cacatcctcg tccaggctgc cctcgatgat gacgacatcg
    12301 acatagatat cgtcgccggc gccgcccctg agctcgtcca tcccgtccga tccgccccac
    12361 agccggtcgg cgccgtcgcc gccgctgatg acgtcgctat aattgctgcc gaggatcaca
    12421 tcgtcgccgc cgctgccttg caaaacatat tggtcggccc agtcgaccga gcgcatgtcg
    12481 agcgtcgagc cgccgacttc gagccggatc gagttggtcc ccagataggc ggacgccttg
    12541 acgtagccgg cggtggtgat ggcgacttgc gtcgccgtga cgttgacgaa ctgcccgaac
    12601 tgggcggcgc tcatggcgag cgcgccggag ctggagatca gctccacgcc cgcgatcgtc
    12661 accgcggaca cgtcgaggaa ggtcgagcca tagaccgcga catgatcgtc gccggcgccg
    12721 ccgatatatt gctcccccgc caccaggttg cgcgcatcga tctgcaaatc atcgtcgccg
    12781 gcgccgccat tgacgatgtc gaggccgagc ccggcatgga tatagtcgtt acccagtccg
    12841 ccgctcagcc tatcgtcccc gtcgcggccg tagagcctgt cggccccgga gccgccgacg
    12901 acgacgtcgg tgccgtcgct gccggcgacc tcctgggctt tctggacgcc ggtcagcttg
    12961 agcgtgatgc cgccgacgcc cagctcgatc gtgcccgccg tgacggcggc gccgctgaga
    13021 tcgcagacgc cggccgtggc gatcgtcagg tcgccgacgg cttccacgaa gacgatactg
    13081 aacgcgccca gcaacccggc gtcgatcgag ctcagatttt cgagccgcag ctcctcgatc
    13141 tggcgggcga tggtggccgg cccggaggcg ctcacgatcc cgctataata taatatgtcg
    13201 tggccctcgc cgccataata gcggtccccg gcgccgcccg ccgcgccggc atggtcgaac
    13261 ttgtccgcgc cgtccccgcc gttcagcaca tccgcgccgg cgccgccggt cagcaggtcg
    13321 ttgccgccgc cgccggtcag cacgtcgtcg ccgccactgc cttcgaaagt ctcggcggcc
    13381 gacgtgccgg tgacgatgtc gcggccgctg ctgccggtca ccctggccgg gccgccgggc
    13441 ccctcgggaa ggtagagcgt gacgccggcc acgccgagct tgatgctgct cgtcaccagc
    13501 tcggtctcgc gcaagtcgac ggcgcccgcg ctggtgatgg tcagctcttt tgccgaaacc
    13561 gcgcggaaag cggcgaactg gcccgctctc agcgacgcgt cgccgagccg gtcggcggac
    13621 ccgacgcgca ggctttcgac gtccgccgcg atcgtcaggc cgctgatgtt gaccccgttc
    13681 acactgatca gcagttcgtc ccaaccggcg ccgccggcat aggattcccc cgccgccaaa
    13741 tcgccttggc cacggatgac gaagcggtcg ttgccggcgc cgccgcttat ggtgtcgcgg
    13801 ccgagcgcgc cggtgatcac atcgtcgccg ccgagcccgt cgatcaggtc gttggccgtg
    13861 gttccgttga tgatgtcatt gccttcggtc gccacgttct tttcccccac tggtcgcgcg
    13921 ggggtcacgg cgcacgtcgc cgccgcgcct ccgaagcgcg ccggcctgcc tcttggaatc
    13981 caa
//
LOCUS       DASVRK010000023         4993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_377055, whole genome shotgun sequence.
ACCESSION   DASVRK010000023 DASVRK010000000
VERSION     DASVRK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377055"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF589_00870"
     CDS             complement(<1..849)
                     /locus_tag="VF589_00870"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX8256155.1"
                     /translation="MSPPNPPYPTEIRRYGKDAIEAAARLIRQGLPVAVPTETVYGLA
                     ADATDGEAVARIYEAKGRPAFNPLIVHVRDLAQAETLGCFSEGARAVAQAYWPGALTL
                     VVPSRPGSGIAALATAGLGSIALRCPRHEVIQALLAETGKPLAAPSANLSGGISPTTA
                     QHVLKTLGGRIKLIIDGGPTALGIESTIVSFAGGGLRLLRPGPVDFGEVAAAAGLPGD
                     SGVPGKIEAPGQLPSHYAPSKPLRLEARSAAPDEWLIGFGEVAGDDSLSPSGDLVEAA
                     ARLFDAL"
     gene            903..2624
                     /locus_tag="VF589_00875"
     CDS             903..2624
                     /locus_tag="VF589_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8256156.1"
                     /translation="MTYSPPIAEQRFLLQHIVKLDEIAGHNAFAEASPDLVDAILEGA
                     GAFAAGEFGPLNRVGDQIGAAWSPDGVAMPPGFKAAYHAYVEGGWGTLAGPPEFGGQG
                     LPMTLATVVLEDLGSANLAFSLCMMLTPGTVEALVRHGSDELQRIWLPKLVTGEWNGT
                     MNLTEPQAGSDVGALKTRAVPKGDGTYRVKGQKIFITWGEHDLTDNIVHLVLARLPDA
                     PAGTRGISLFLVPKFRLGPDGAPAEFNDARCVSIEHKLGIHASPTCVMSYGDQDDCVG
                     WLVGEENGGMRAMFTMMNNARLNVGLQGVSIAERATQAAVAYARDRRQGARGLGREAA
                     AIVEHPDVRRMLMRMKALTQAARALTYYAAGQTDRGALGDAAAKARLDLLTPLVKAYG
                     TDVGVEVASLGVQVHGGMGYIEETGAAQHYRDARITPIYEGTNGIQAADLVGRKLGLE
                     GGAVLERLLADMRADTGDLPQLGALIAACEAAARRLAAAAPDDRLAASYPFMTMLSVA
                     VAGWLMARQLAAAAAGEGDPAFLAMKRAAALFYIERLVPEALGLEAQAAASADLLYSV
                     PEEAFAA"
     gene            2689..2862
                     /locus_tag="VF589_00880"
     CDS             2689..2862
                     /locus_tag="VF589_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256157.1"
                     /translation="MAEDDKGVGEGGAQSGEAAAPGAPAKGDSLEDAANEAVREIERR
                     EDGGGHAGAGDIG"
     gene            3000..3491
                     /locus_tag="VF589_00885"
     CDS             3000..3491
                     /locus_tag="VF589_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256158.1"
                     /translation="MSLPLLVGGCVADGHFYGGAESGGGFRQEAEGSATIYVHTPVGG
                     APESAGASRRGPDPRAVAEVAATAARQVRRCYRQPRIRHEGRRIVTRLLVRFAPDGSL
                     TELPSVLEQHGVTASSRAHAAAMAEAASLAVIRCAPVRLPPELHKGGWDVLQLTFSPQ
                     ARA"
     gene            complement(3752..4099)
                     /locus_tag="VF589_00890"
     CDS             complement(3752..4099)
                     /locus_tag="VF589_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8256159.1"
                     /translation="MIKDDRPVYLRLRDSIAAGILDGRVGDGDPLPSVRSLAAEHGAN
                     PLTVAKAYQTFQDEGLVVVKRGVGMFVAEGATARLRTSARKEFLEQRWPAIAAHIRRL
                     DLDVTDLVDRALA"
     gene            complement(4159..4743)
                     /locus_tag="VF589_00895"
     CDS             complement(4159..4743)
                     /locus_tag="VF589_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX8256160.1"
                     /translation="MLNDRSTALSLLKTRRSGRPRDLVAPGPDDEQLREMFEIACRTP
                     DHGKLVPWRFIHVRKQDRGAFAALLHRAHRIGCPEPTRAEIEAVDRMAHQAPTLVVVL
                     SSPVQPSKIPVWEQQLSCGAAIMNLLLAAHASGYAGGWITGWAAYSEEVRRAFGRDHE
                     SIAGFVYIGTPANPLEERPRPAPELAVSDWTPAG"
BASE COUNT          735 a         1644 c         1865 g          749 t
ORIGIN      
        1 gagggcgtcg aacagtcgcg cggcggcctc gacgaggtcg cccgacgggc tgaggctgtc
       61 gtcgccggcc acctcgccga acccgatcag ccattcgtcg ggcgcggccg agcgcgcctc
      121 gaggcgcagc ggcttggagg gcgcataatg gcttggcagc tggccgggcg cctcgatctt
      181 gcccggaacc ccgctatcgc cgggtaatcc ggctgccgcc gccacctcgc cgaagtcgac
      241 cggaccgggc cggagcaggc ggagaccgcc gcccgcgaag ctcacgatcg tggactcgat
      301 gccgagtgca gtcgggccgc catcgatgat cagctttatc cgcccgccga gggtcttcag
      361 gacatgctgc gcggtggtcg ggctgatgcc gccgctcaga ttggcggaag gcgcggcgag
      421 cggcttgccc gtttccgcca gcagcgcctg gatcacctcg tggcgcgggc agcgcagggc
      481 gatgctgccc agaccggcgg tggcgagcgc ggcgatgccg ctgcccgggc ggctcgggac
      541 gaccagggtg agcgcgcctg gccaataagc ttgtgcgacg gcgcgagcgc cctcggagaa
      601 gcagcccaaa gtttcggcct gcgcaagatc gcggacgtgg acgatcagcg ggttgaaggc
      661 gggcctgccc ttggcctcgt agatacgcgc caccgcctcg ccgtcagtgg cgtcggcggc
      721 gaggccgtag accgtctcgg tcgggaccgc gaccggaagg ccctgccgga tgaggcgcgc
      781 cgccgcctcg atcgcgtcct tgccgtagcg gcggatttcg gtcggataag gcgggtttgg
      841 cgggctcacc cggcggcgct atagccgggg ccgaactcac gcaagaccgg ggacccgacg
      901 ccttgaccta cagcccgccg atcgccgaac agcgtttcct gctccagcat atcgtcaagc
      961 tcgacgagat tgccggccac aatgccttcg ccgaggcgag ccccgatctg gtcgacgcca
     1021 tcctcgaagg ggcgggggcg ttcgcggccg gcgagttcgg gccgctgaac cgggtcggcg
     1081 accagatcgg cgcggcttgg tcgccggacg gcgtcgcgat gccgccgggc ttcaaggcgg
     1141 cctaccacgc ctatgtcgag ggcggctggg gcacgctggc ggggccaccc gaatttggcg
     1201 gccaagggct gccgatgacg ctggcgacgg tggtgctcga ggatctcggc tcggccaatc
     1261 tggctttctc tttgtgcatg atgctgacgc ccggcacggt cgaggcgctg gtccggcacg
     1321 gctcggacga gctgcagcgg atctggctgc ccaagctggt gaccggcgaa tggaacggca
     1381 ccatgaacct caccgagccg caggccggct ccgacgtcgg cgcgttgaag acgcgcgcgg
     1441 tgccgaaggg cgacggcacc tatcgcgtca aggggcagaa gatcttcatc acctggggcg
     1501 agcacgatct taccgacaat atcgtccatc tcgtgctggc gcggctgccg gacgcgcccg
     1561 ccggcacccg ggggatttcg ctgttcctgg tgccgaagtt ccggctcggc ccggacgggg
     1621 cgccggctga gttcaacgac gcgcgctgcg tgtcgatcga gcacaagctc ggcatccacg
     1681 cctcgcccac ctgcgtcatg tcctatggcg accaggacga ttgcgtcggc tggttggtcg
     1741 gcgaggagaa tggcggcatg cgggcgatgt tcacgatgat gaacaatgcc cgcctcaatg
     1801 tcggccttca gggcgtgtcg atcgccgagc gggcgaccca ggccgcggtc gcctatgcgc
     1861 gggaccggcg gcagggggcg agggggcttg gccgcgaggc ggcggcgatc gtcgagcatc
     1921 ccgacgtgcg gcggatgctg atgcggatga aggcgctgac ccaggcggcg cgggcgctga
     1981 cttattatgc cgccggccag accgaccgcg gcgcgctcgg cgacgccgcc gccaaggcgc
     2041 ggctcgacct gctgacgcct ttggtcaaag cctacggcac cgatgtcggc gtcgaggtgg
     2101 cgagcctcgg cgtgcaggtc catggcggca tgggctatat cgaggagacc ggcgccgctc
     2161 agcattatcg cgacgcccgc atcacgccga tctacgaagg caccaacggc atccaggcgg
     2221 cggacctcgt tggccgcaag ctcgggctcg aaggcggggc cgtgctggag cggctgctcg
     2281 ccgacatgcg cgccgacacg ggcgatctgc cgcagcttgg tgcgctgatc gccgcctgcg
     2341 aggcggcggc gcgccggctc gcggcggccg cgcccgacga ccggctcgcc gcctcctatc
     2401 cgttcatgac gatgctgtcg gtcgcggtcg ccgggtggct gatggcgcgc cagctcgccg
     2461 ccgccgcggc cggcgagggg gatcccgcct tcctggccat gaagcgcgcc gccgcgcttt
     2521 tctatatcga gcggctcgtg cccgaagcgc tcgggctgga ggcgcaggcg gcggcttcgg
     2581 cggacctgct ctattcggtg cccgaggagg cgttcgccgc ctgaggcggc gatgcaacag
     2641 gcgccggctc ggctcgttct tggggaaggg atttcgcgag gaaacggcat ggcagaggat
     2701 gacaaaggtg tgggcgaagg tggtgcccaa agcggcgagg ccgcggcccc gggcgctccg
     2761 gccaagggcg attcgctcga agatgccgcc aacgaggcgg tgcgggagat agagcggcgc
     2821 gaggatggcg gcggccatgc cggagcgggc gatatcggct gagcgccgcg ccggcgtcgg
     2881 gcggcgaaaa atctttttgc ctgcggtggc ttaacctacg ataagcttga gccgacgctc
     2941 caacgaatcg ccccgcacga cccatgtcag cttccagttt attcagatcg ccgctcctgt
     3001 tgtcgctgcc gctgctcgtg ggcggctgcg tcgccgacgg gcacttttac ggcggagcgg
     3061 aaagcggcgg cggtttccgg caggaggcgg agggaagcgc cactatctac gtccacaccc
     3121 cggtcggcgg cgctccggag agtgccggcg cttcgaggcg cggacccgat ccgcgggcgg
     3181 tggcggaggt cgcggcgacg gcggcgaggc aggtgaggcg ctgctaccgg cagccccgga
     3241 tccggcacga agggcgccgg atcgtcaccc gcctgctggt gcggttcgcg cccgacggca
     3301 gcctgaccga gcttccgtcc gtgctcgagc agcatggcgt gaccgcgtcc agccgcgccc
     3361 atgccgccgc catggccgag gcggccagcc tcgcagtgat ccgctgcgct ccggtgcgcc
     3421 tgcctcccga gctgcacaag ggcggctggg acgtgctgca actcaccttc agcccgcagg
     3481 cgcgggccta ggggggctgg cggcgcgatc ccgctcgatt ccgttcgtgt cgagcgaagt
     3541 cgagacaccc catcgagcgc agtcgagaac ctgaacccga tgacgcctcg gcttcgctcg
     3601 gcagggtccc tcgactccgc tcgggacgaa cggaaccccg cgaacgcggg aatcccgctg
     3661 ctttaagcca gagggcaggg aagaagagaa gcgggatccc cgctttcgcg ggaatgacgg
     3721 agtggctcgg gtgacgggag tatgcccccg ctcaggcgag ggcgcggtcg accaggtcgg
     3781 tgacgtcgag gtcgaggcgg cggatatggg cggcgatggc gggccagcgc tgctcgagga
     3841 attccttgcg ggcgctggtg cgcagccggg cggtggcgcc ctcggcgacg aacatgccga
     3901 cgccgcgctt caccaccacc agcccctcgt cctggaaggt ctgataggcc ttggcgaccg
     3961 tgagcgggtt ggcgccatgc tcggcggcga ggctgcggac cgacggcagc ggatcgccgt
     4021 cgccgaccct tccgtcgagg atgccggcgg cgatcgaatc gcgcaggcgg agatagacgg
     4081 ggcggtcgtc cttgatcatg ccctgctatg ctgtactaat acaccgcgcc ggtcaagccc
     4141 gcaggtgatg gccccgcctc agccggcggg cgtccagtcg ctgaccgcca gctccggcgc
     4201 cggccgcggc cgctcctcga gcggattggc cggggtgccg atatagacga agccggcgat
     4261 gctctcgtga tcgcgtccga aggcgcggcg cacctcctcc gaataagcgg cccagccggt
     4321 gatccagcca cccgcatagc cgctggcatg cgcggcgagc agcaggttca tgatcgccgc
     4381 gccgcacgac aattgctgct cccacacggg gatcttggac ggctggaccg ggctcgaaag
     4441 caccaccacc agggtcggcg cctgatgcgc catccggtcg accgcttcga tctcggcgcg
     4501 ggtcggctcg gggcagccga ttcgatgggc gcggtggagg agggcggcaa aggcgccgcg
     4561 atcctgcttg cggacgtgga tgaagcgcca ggggacgagc ttgccatggt cgggcgtgcg
     4621 gcaggcgatc tcgaacatct cgcgcaattg ttcgtcgtcg ggacccgggg cgacgaggtc
     4681 gcgcggccgg cccgagcggc gggtcttgag gagcgagagg gcggtggagc ggtcgttgag
     4741 catcgccgcg gcgctagcag cgtggcggcc cggcaacaat gtgcttcaca tgttcgtttt
     4801 ggccgctagg ttgcgcgccc aatgaggcac gcgcgtgccc gataagtaca tgaggatgct
     4861 tgctatgggc ctgaaggacg tctccttgtg gaatgaaggc gattggatcg cggtggtggc
     4921 cttggtcctt ctggccgggt ttctctcggt cggcggcctg atcgcggtca gcaagaagcc
     4981 ggagttcgcg ggc
//
LOCUS       DASVRK010000024        12813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_253100, whole genome shotgun sequence.
ACCESSION   DASVRK010000024 DASVRK010000000
VERSION     DASVRK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253100"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..669
                     /locus_tag="VF589_00900"
     CDS             235..669
                     /locus_tag="VF589_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8256161.1"
                     /translation="MPSPRRPIPAFAIPAEQPIGELNTTPLIDVMLVLLIMFIVTIPI
                     TTHKVDIDIPQSGGVPAKPVVHRLELDAAGRAHWDDAPVARSQLPALVRAMNSEPNAE
                     LHLRADGETPYAAFDEVLAIVKRVGVERLGMVGNERFVHAAS"
     gene            739..972
                     /locus_tag="VF589_00905"
     CDS             739..972
                     /locus_tag="VF589_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256162.1"
                     /translation="MVLLIVGALLVVLGVVVAARSNVKRGGRMSQSEETVSHEPRDTL
                     EPTGRGDRLSFKPDLPGMVLIGLGLILMVVGAG"
     gene            1094..2227
                     /locus_tag="VF589_00910"
     CDS             1094..2227
                     /locus_tag="VF589_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX8256163.1"
                     /translation="MRLSKTITQLNRARRHMAAASPAGPAADRLSDFTGFENPGSLRG
                     RCYVPANLPAGAALVVVLHGCTQTAGGYDHGAGWSHLADRHGFALLFPEQQRANNPNL
                     CFNWYQPADAKRGMGEAASIRAMVEAMRKAHGLDPARIYVTGLSAGGAMASVMLAAYP
                     EVFAGGAIIAGLPYGCASNVSEAFECMAGRAAGDSAALAASVRRASPHKGPWPRVSVW
                     QGSADRLVVPGNADAILAQWTGLHGLAATPDRIDTVGRHPRRVWLGAEGEVLIEHFDI
                     AGMGHGTPLMPGTEEGESGNAGAHMLDVGLSSTDHIAAFFGIAPPPPAATRRKPAAKA
                     TAAAAPAPAPRKRAAPRRNPKPEPASGVQAVIEDALRAAGLLR"
     gene            2577..2936
                     /locus_tag="VF589_00915"
     CDS             2577..2936
                     /locus_tag="VF589_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256164.1"
                     /translation="MTAYAAERWQHAEGVFTPLELQVIALAAGSPGDCRVSTTDGGLL
                     ARIGRALAKLFALHIPAGLANERLEALRRLACRSFATGGRLEQETIAKALAAGLNRLQ
                     VDTVAGIAAGWHANLTR"
     gene            3024..4928
                     /locus_tag="VF589_00920"
     CDS             3024..4928
                     /locus_tag="VF589_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX8256165.1"
                     /translation="MRIATVFAAAALLIAGCSGPSGDQAKQAEGERMVAPILTSEGAR
                     DVHSFARPEIARVTHVALDLVADFAAKRIGGTATLDIQAAKGAGEIVLDTKGIEIASV
                     TDADGRPLRWALGEGDAAKGRPLSVKMGAARQLSIAYKSGPDAEALQWLTPAQTVGKR
                     HPYLLSQGQPTLNRTWIPTQDSPGIRQTWEARVVAPEPLKVVMSGERLTPQGEPAGQG
                     RRAYRFRMDRPVAPYLIAIAAGDIAFRPLGPRTGVWAEPGMIEAAARELADTERMVEA
                     AEKLYGRYRWGRYDVIVLPPSFPYGGMENPTLTFLTPTFIAGDKSLVGLVAHELAHSW
                     SGNLVTNATWPDGWLNEGFTSYFENRIMEALYGPRRAAQEAALSWADMESAFTELGGA
                     GAPGTRLRGDDGPDDGASGIVYDKGAIFLRSLERIVGRERWDAYLRSYFDRHAFQPMT
                     SARFLADLCANLVKGDAELERKLMLDQWVYQPGLPANAARPDPQAFAQVDQATRAYVT
                     GGPVAQVPFAQWNTAERLRFLNALPRELPPQRLAELDRGFGLSRAGNNEVLFAWLKLA
                     IANRYDPAVPAAERFLAGMGRRKFVAPLFEALAAEGEWGKPIARRIYAAARPTYHSVT
                     TGTVDKLLAS"
     gene            5167..7452
                     /locus_tag="VF589_00925"
     CDS             5167..7452
                     /locus_tag="VF589_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8256166.1"
                     /translation="MPDAELPYDDGNHRLREAHLHNGQAQTTALFAPYFALAGISSAL
                     LVAWAMLGHGRIYLIVPWVATVAFGHWVSYRRAMEAGSAAGSRSARRQAHWRPVVEAA
                     ALAALWASLPTYAFAVHPPEVQVVIAGAMGALISAAIALAAIPAAALAWISVITLALC
                     IAYYFGAATIDLKLGLTIVGFAAIAIFGVGRFTRWTFAQLRDMAKVRTQAESVRLLLR
                     EYEHRGVGWLWQVDSDNRVVYISSRMTSLLGRSTSQLIGHSLPASLGGNSALGRTLLE
                     RQPFANLEMELKTRRGPRWISLAGDPIIDMAGHFQGFRGVGSDITEVRKTQERLTNLA
                     NMDVLSGLPNRGRVRQLLGEALSNAVATNVPCAIMFLDLDGFKPVNDTFGHPKGDAVL
                     KSVAQRLVKAVGDAGHVGRMGGDEFAIVIRDAQGRKTVENLANRLIESIAQPYHIDKV
                     EIRIGVSIGCAYGPIDGQTVDDLIQKADMALYQAKNAGRGTCCYFNDDMRSVAEHKLK
                     LEQDLSNAIANGQLRLLYQPLISAADQSLVGFEALIRWHHPTRGVVSPVEFIPLAEET
                     GLIMHLGDWVIEEACRAAATWPDNITVAVNLSARQLVLPALPATVNGALSKYRVKANR
                     LELEVTETVFLEDTAGSLDVLKRLRALGVGIALDDFGTGYSSLGYLNKAVFHKLKIDG
                     SFVREAANNKETVAIIQSIVQLAKSFRMTITAEGVETADDFTRMRDLGCHQIQGYLFG
                     RPMTFERASELVHGTQSRMTA"
     gene            complement(7484..8506)
                     /locus_tag="VF589_00930"
     CDS             complement(7484..8506)
                     /locus_tag="VF589_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8256167.1"
                     /translation="MPELTVAAGLAKGLIGVAVAAGASAAELAERSGIALAALDDQDA
                     RIPLDQYVALMRSAKTLTGDPALALRYGATVHMSAFSVVGLIFYTCETIAESIEQVNR
                     YGQLIVETGVGAAPRFQFERREGRVWMVDTRPDPNAFPELTEATFARFISMTRPFSDG
                     ALAQEMHVSHSAPAYWRDYERICGVPVTFEAGWNAVRVDEGRLTQRIATQPRYAFGIL
                     SEHAEALLVSLESSKTVRGRVEGLAMPILHTGKVSVEAIAREMGVSRQTLFRKLKAEG
                     TSFEQVLDELRHKLALHYLAGRKASVHETAYLVGFSDRAAFSRAFKRWTGRSPSALRG
                     GKDEDA"
     gene            8625..9338
                     /locus_tag="VF589_00935"
     CDS             8625..9338
                     /locus_tag="VF589_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256168.1"
                     /translation="MTLIVHILFGSAALAAGAAGLWFRKGSRAHAAAGTIFFVAMLVM
                     AGTGALVAALGSERGTAVIGLFTLYLVATSWATARRRDGTAGRFEVAGLLVASACAAA
                     FTACAVVAANSASGRLDSLPAAAHYPFAIVAALAAGLDLNFILRRRLLPRQRLARHLW
                     RMCAAFLIAAFSFFMGQQKVMPDFMRGSLALFLPEIAILGAMTYWLVRLRLGKKYARL
                     VPARAAASPGQPLAPAKPA"
     gene            9335..10060
                     /locus_tag="VF589_00940"
     CDS             9335..10060
                     /locus_tag="VF589_00940"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX8256169.1"
                     /translation="MSGATPDPRHGSARLTGLLYVLSNITAIFAFWVRSRLVAGNDPA
                     RTADNIMASEPLFRAGLASELVTIVLTTALVLGLYVVLKPVGRNLASLALLWRILENS
                     VLAALTFASFTALALLGGGAYLASVDTAQAEGLAYALIRVHIYGFQAGFLFLGLGSAL
                     FSYLWLKSRYIPPLIAAWGIFASLLMASVSLALIVMPGLISVITLAYMLPMGLYEIGL
                     GLWLVAKGIRLPEQARPSADEAE"
     gene            10408..12243
                     /locus_tag="VF589_00945"
     CDS             10408..12243
                     /locus_tag="VF589_00945"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX8256170.1"
                     /translation="MDESRAESDSKSVAPIRFKVKTDQSRDALLTEFGKDTLRDRYLL
                     PGETYQDLFARVASAYADDAAHAQRVYDYISRLWFMPATPVLSNGGTGRGLPISCYLN
                     SVDDSLNGIVNTWNENVWLAARGGGIGTYWGAVRGIGEPVGLNGKTSGIIPFVRVMDS
                     LTLAISQGSLRRGSAACYLDINHPEIEEFLEIRKPSGDFNRKALNLHHGVLVTDAFME
                     AVRDGDEWILRSPKDGSERARVDARALFQKLVETRLATGEPYIIFIDKVNRSMPRHQR
                     ELGLKVSTSNLCSEITLPTGRDHLGNDRTAVCCLSSLNLETWDEWNKDKVFIEDVMRF
                     LDNVLQDYIDRAPAEMARAKYSAMRERSVGLGVMGFHSFLQARNIAFEGAMAKSWNLK
                     IFRHISAQVNEASMMLANERGPCPDAADQGVMERFSCKMAIAPTASISIICGGTSACI
                     EPIPGNVYTHKTLSGSFSIRNPHLERLLIAKAKNSEAVWNSILEKNGSVQHLDFLTAE
                     EKDTFKTSFEIDQRWLLELAGDRTPYIDQATSLNLFIPADVDKWDLLMLHYRAWELGI
                     KSLYYLRSKSVQRAGFAGGVEADNTPDLKQIELQTTDYDECLACQ"
     gene            12329..>12813
                     /locus_tag="VF589_00950"
     CDS             12329..>12813
                     /locus_tag="VF589_00950"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8256171.1"
                     /translation="MSLTEARKQYKPFEYPWAFDFWKRQQQIHWMPEEVPLGEDCRDW
                     AQKLTEHERSLLTQIFRFFTQADVEVQDCYHDKYGRVFKPTEIKMMLTAFSNMETVHI
                     AAYSHLLDTIGMPESEYSAFLQYKEMKDKHDYLATFGVDTDEDIAKTLAMFGGFTEGL
                     QL"
BASE COUNT         2041 a         4808 c         3919 g         2045 t
ORIGIN      
        1 atagcctgaa cgcgcgaaca gcgaaatcgt aggcgcggac cggccgccgt ccccctgaac
       61 gtccgccgtc accctgaccg gcccgtaacc tgaaccgcct ccgtcaccgc cgtcgacgtc
      121 acctgaatga cagctctacg cctccgccgg ttgcgtcacc ctgaacagca tgacagccgc
      181 ttgtgccgcc gctctgtcga tggtacaata tctcataaca tgaaggagaa ggtcatgccc
      241 agcccgcgcc gcccgatccc ggctttcgca attcccgccg agcagccgat cggggagctc
      301 aacaccacgc ctttgatcga cgtcatgctg gtgctgctga tcatgttcat cgtcaccatc
      361 ccgatcacca cccacaaggt cgacatcgat attccccaga gcggcggggt ccctgccaag
      421 ccggtcgtcc accggctcga actcgatgcc gccgggcggg cgcattggga cgacgcgccg
      481 gtcgcccgct cccaactgcc cgccttggtc cgcgccatga acagcgagcc gaatgccgag
      541 ctgcacctgc gcgccgacgg cgagacgccc tatgccgcct tcgacgaggt gctcgcgatc
      601 gtgaagcggg tcggcgtcga gcggctcggc atggtcggca acgagcgctt cgtgcacgcc
      661 gcctcctgag cggccccgcg tttccctccc gcgccggttt gcgctaagca gggggctagg
      721 ctcagccggg aggcgatcat ggtcctgctc atcgtcggtg cgttgctcgt ggtcctgggc
      781 gtggtcgtcg ccgcccgctc gaacgtcaag cgcggcggcc gcatgagcca gtcggaagag
      841 accgtgtcgc acgagccgcg cgacaccctg gagccgaccg gccgcggcga tcgcctcagc
      901 ttcaagcccg atcttcccgg catggtcctg atcgggcttg gcctgatcct gatggtggtc
      961 ggcgccggct gaggcccgcc cgcatccttg atccacatta aaatgctgca ctgcagcaac
     1021 gttcggtgca acaaggcgtt ctatcgtcga cctcgtccgt tgtggacgcg gcgcctgtgc
     1081 gggaaggaac ccgatgcgcc tctcgaaaac catcactcaa ctcaatcgcg cgcgccgcca
     1141 tatggccgcc gcctcgccgg ccggcccggc cgccgaccgc ctgtccgatt tcaccggctt
     1201 cgaaaatccc ggcagcctgc gcggccgctg ttatgtcccc gcgaacctcc ccgcaggcgc
     1261 cgcgctcgtc gtcgtgctcc acggctgcac ccagacggcc ggcggctacg atcatggcgc
     1321 cggctggtcc cacctcgccg accgccacgg cttcgctttg ctctttcccg agcagcaacg
     1381 cgccaacaat cccaatctct gcttcaactg gtaccagccc gccgacgcca agcgcggcat
     1441 gggcgaggcc gcctcgatcc gcgccatggt ggaagcgatg cgcaaggccc acggcctcga
     1501 tcccgcgcgg atctacgtca ccggcctgtc ggcgggcggg gcgatggcct cggtgatgct
     1561 cgccgcttat cccgaagtct tcgccggcgg cgccatcatc gccggccttc cttatggctg
     1621 cgcgtccaac gtgtccgagg cgttcgaatg catggccggc cgcgccgccg gcgatagcgc
     1681 ggcgctcgcc gccagcgtcc gccgcgcctc gccgcacaag gggccgtggc cgcgcgtgtc
     1741 ggtgtggcag ggcagcgccg accgcctcgt cgtgcccggc aatgccgacg ccatcctcgc
     1801 ccaatggacc ggcctccacg gcctcgccgc cacgcccgac cgcatcgaca cggtcggccg
     1861 ccatccccgc cgcgtctggc tcggcgccga gggcgaagtg ctgatcgagc atttcgacat
     1921 tgccggcatg ggccacggca ccccgctcat gcccggcacg gaggagggcg agtccggcaa
     1981 tgccggcgcg cacatgctcg acgtcggctt gagctcgacc gaccatatcg ccgccttctt
     2041 cggcatcgcc ccgccgccgc ccgcggccac ccgccgcaag cccgccgcca aggcgaccgc
     2101 cgcagcggcc ccggctccgg cgccccgcaa acgcgccgct ccccgccgaa acccaaagcc
     2161 cgaacccgcc tcaggcgtcc aggccgtaat cgaagacgcc ctccgcgccg ccggcctgct
     2221 ccgctagccg gcagcgttgg tccggccccg ccggggagaa ggaacacccc ccccgccggg
     2281 gacaaggaac aaccgtcatg ccagcgaaag ctggcatctc acttcttttg ggagccaggg
     2341 aaaaagggag attccagctt tcgctggaat gacggcggcc cgcgtcttgc acagcctccg
     2401 ggcgtcgcga catgttcccc ggcgaaagcc ggggtccagc ttccttactt ccgtccgaac
     2461 ccgttgctaa ttccgcaaca ccaatgttcc aggcgcgcct cttccgcatc ctcctcggca
     2521 accctaaatc ccctcctgcc gccgcgcgcg gcgcagccaa aaggggagat gacgccatga
     2581 ccgcttatgc cgccgaacgc tggcagcacg ccgagggcgt cttcaccccg ctcgagctgc
     2641 aggtcatcgc gctggccgcg ggctcgcccg gcgattgccg cgtgtcgacc acggacggcg
     2701 gcctgctcgc ccggatcggc cgcgcgctcg ccaagctgtt cgcgctccac atccccgccg
     2761 gcctcgccaa cgagcggctc gaggcgctgc ggcggctcgc ctgccgcagc ttcgccaccg
     2821 gcggccggct cgagcaggag accatcgcca aggcgctcgc cgccggcctg aaccggctcc
     2881 aggtcgacac cgtcgccggc atcgccgccg gctggcacgc caacctcacc cgctagcggc
     2941 gatccggttg cagggccgcg gcccgccgcc taggtaaggc gctcccgcca accagcccag
     3001 gtcccgcctt gccccgtccc aacatgcgta tcgccacggt tttcgccgcc gccgccctcc
     3061 tgatcgccgg ctgctccggc ccctccggcg atcaggccaa gcaagccgag ggcgagcgca
     3121 tggtcgcgcc aatcctcacc agcgaggggg cgcgcgacgt ccacagcttc gcccggcccg
     3181 agatcgcccg cgtcacccat gtcgcgctcg acctcgtcgc cgacttcgcc gccaagcgga
     3241 tcggcggcac cgccacgctc gacatccagg ccgctaaggg cgccggcgag atcgtgctcg
     3301 acaccaaggg catcgagatc gcctccgtca ccgacgccga cggccgcccg ctgcgctggg
     3361 ccttgggcga gggcgacgcc gccaagggcc gcccgctcag cgtcaagatg ggcgccgccc
     3421 gccagctcag catcgcctat aagagcgggc ccgatgccga ggcgctgcaa tggctgaccc
     3481 ccgcccagac cgtcggcaag cgccacccct atctgctgag ccagggccag ccgacgctca
     3541 accgcacctg gatcccgacc caggacagcc ccggcatccg ccagacctgg gaagcccgcg
     3601 tggtcgcgcc cgagcccttg aaggtcgtca tgtccggcga gcggctgacg ccccagggcg
     3661 agcccgccgg ccagggccgc cgcgcctatc gcttccggat ggaccggccg gtcgcgcctt
     3721 acctgatcgc catcgccgcc ggcgacatcg ccttccgccc gctcggcccg cgcaccggcg
     3781 tctgggccga gcccggcatg atcgaggccg ccgcgcgcga gctcgccgac accgaacgca
     3841 tggtcgaggc ggcggagaag ctttacggcc gctaccgctg gggccgctac gacgtgatcg
     3901 tcctgccgcc gagcttcccc tacggcggca tggagaaccc gaccctcacc ttcctgacgc
     3961 cgaccttcat cgccggcgac aagagcctgg tcggcctcgt cgcgcacgag ctcgcccata
     4021 gctggtccgg caacctcgtc accaacgcga cctggcccga cggctggctc aatgagggct
     4081 tcacctccta tttcgagaac cgcatcatgg aggcgctcta cggcccccgc cgcgccgcgc
     4141 aggaggcggc cttgtcctgg gccgacatgg aaagcgcctt caccgagctt ggcggcgccg
     4201 gcgcgcccgg cacccggctg cgcggcgacg acggccccga cgacggcgcc agcggcatcg
     4261 tctacgacaa gggcgcgatt ttcctgcgct cgctcgagcg gatcgtcggc cgcgagcgct
     4321 gggacgcgta tctgcgctcc tatttcgatc gccacgcctt ccagccgatg acctcggcgc
     4381 gcttcctcgc cgatctttgc gccaatctcg tcaagggcga cgccgagctt gagcgcaagc
     4441 tgatgctcga ccaatgggtc taccagcccg gcctgccggc caacgccgcc cggcccgatc
     4501 cgcaggcctt cgcccaggtc gaccaggcca cccgcgccta tgtcaccggc gggccggtcg
     4561 cgcaggtgcc gttcgcgcaa tggaacaccg ccgagcggct ccgcttcctg aacgcgctcc
     4621 cccgcgagct gccgccccag cggctggccg agctcgaccg cggcttcggc ctcagccggg
     4681 ccggcaataa cgaagtcctg ttcgcctggc tgaagctcgc catcgccaac cgctacgacc
     4741 cggcggtgcc ggcggcggag cgcttcctgg ccggcatggg gcggcgcaag ttcgtcgcgc
     4801 ccttgttcga agcgctggcg gccgagggcg agtggggcaa gccgatcgcg cgccgcatct
     4861 atgcggccgc ccgcccgacc taccattcgg tcaccaccgg caccgtcgat aagctgctcg
     4921 cctcctagcc ggctcgcctt gcgccagggg agggggaggg cggttttggt tcgcccgggc
     4981 cgcgccgtcc gcccctcggc ggcggccccg cccggcaact tgatcggtca gaattaacgg
     5041 cgcctttaac actccatcaa gcctatctcg ataacaaccg ggcatccgac cagatgacat
     5101 tggattccca tgtatcaagc gcctgtctca gtagatgccg cgcccgatga gctcgctccg
     5161 ggagacttgc ccgacgcgga gctcccctat gacgatggca accatcgcct gcgcgaggcg
     5221 catctccaca acggccaggc ccagaccacg gccctgttcg ccccctattt cgcgcttgcc
     5281 gggatcagct cggcgctgct cgtcgcctgg gcgatgctcg gccacggccg catctatctg
     5341 atcgtaccct gggtcgcgac cgtcgccttt gggcattggg tatcctatcg ccgcgccatg
     5401 gaggccggct ccgccgccgg cagccgcagc gcccgccgcc aggcccattg gcgcccggtg
     5461 gtcgaggccg ccgctctggc cgcgctctgg gcctcgctcc cgacctacgc cttcgccgtc
     5521 cacccgcccg aagtgcaggt ggtgatcgcc ggcgcgatgg gcgccttgat cagcgccgcc
     5581 atcgcgctcg ccgccatccc ggccgccgcg ctcgcctgga tctcggtgat cacgctcgcg
     5641 ctctgcatcg cttattattt cggcgccgcc accatcgatc tgaagctcgg cctcaccatc
     5701 gtcggcttcg ccgccatcgc catcttcggc gtcggccgct tcacccgctg gaccttcgcc
     5761 cagctgcgcg acatggccaa ggtccgcacc caggccgaat cggtccggct gctgctcagg
     5821 gaatatgagc atcgcggcgt cggctggctc tggcaggtcg atagcgacaa ccgcgtcgtc
     5881 tacatctcgt cccgcatgac cagcctgctc ggccgctcga ccagccagct gatcggccac
     5941 tcgctgccgg cctccttggg cggcaacagc gcgctcggcc gcaccctgct cgagcgccag
     6001 cctttcgcca atctcgaaat ggagctcaag acccgccgcg gcccgcgctg gatcagcctc
     6061 gccggcgatc cgatcatcga catggccggc catttccagg gcttccgcgg cgtcggctcg
     6121 gacatcaccg aggtccgcaa gacgcaggag cggctcacca acctcgccaa tatggacgtg
     6181 ctgtcgggcc tgcccaatcg cggccgcgtc cgccagctgc tcggcgaggc gctttccaac
     6241 gcggtggcga ccaacgtccc ctgcgcgatc atgtttctcg atctcgacgg cttcaagccc
     6301 gtcaacgaca cgttcggcca ccccaagggc gacgccgtgc tgaagagcgt ggcccagcgg
     6361 ctggtcaagg cggtcggcga cgccggccat gtcggccgca tgggcggcga cgaattcgcg
     6421 atcgtgatcc gcgacgccca gggccgcaag accgttgaga atctcgccaa ccgcctgatc
     6481 gaatcgatcg cccagcctta ccatatcgac aaggtcgaga tccggatcgg cgtctcgatc
     6541 ggctgcgcct acggcccgat cgacggacag accgtcgacg acctcatcca gaaagccgac
     6601 atggccctct accaggccaa gaatgccggc cgcggcacct gctgctactt caacgacgac
     6661 atgcgcagcg tcgccgagca caagttgaag ctcgaacagg acctgtccaa cgccatcgcc
     6721 aacggccagc tgcgcctgct ctatcagccg ctgatcagcg ccgccgacca gagcctggtc
     6781 ggcttcgagg ccctgatccg ctggcaccac ccgacccgcg gcgtcgtctc gccggtcgag
     6841 ttcatcccgc tcgccgagga aaccggcctc atcatgcacc tcggcgattg ggtgatcgag
     6901 gaggcctgcc gcgccgccgc gacctggccc gacaacatca ccgtcgcggt caacctgtcc
     6961 gcccgccagc tcgtcctgcc ggctttgccc gccaccgtga acggcgcgct ctccaaatac
     7021 cgtgtcaagg ccaaccgcct cgagctcgaa gtcaccgaga ccgtcttcct cgaagacacg
     7081 gccggctcgc tcgacgtgct gaagcgcctg cgcgcgctcg gcgtcggcat agcgctcgac
     7141 gatttcggca ccggctattc gtcgctcgga tatctgaaca aggccgtgtt ccacaaattg
     7201 aagatcgacg gctcgtttgt ccgcgaggcg gcgaacaaca aggagacggt ggcgatcatc
     7261 cagtcgatcg tgcagctcgc caagagcttc cgcatgacca tcaccgccga gggcgtcgaa
     7321 accgccgacg atttcacccg gatgcgcgac ctcggctgcc accagatcca gggctatctg
     7381 ttcggccgcc cgatgacctt cgaacgcgcc agcgagctcg tccacggcac ccagagccgc
     7441 atgaccgcct gacccgctcg cggggccaat ccgaggggcg ccgctaggcg tcctcgtcct
     7501 tgccgccgcg cagcgcgctt gggctccggc cggtccaccg cttgaacgcg cgcgagaagg
     7561 cggcccggtc cgaaaagccg accagataag cggtctcgtg caccgacgct ttcctgcccg
     7621 ccagataatg cagcgccagc ttgtggcgca gttcgtccag cacttgctcg aatgaggtgc
     7681 cttccgcttt cagcttccgg aacaaggtct ggcggctgac gcccatctcg cgcgcgatcg
     7741 cttccacgct caccttgccg gtgtggagga tgggcatcgc cagcccctcc acgcgcccgc
     7801 gcaccgtctt ggagctttcg aggctcacca gcagcgcctc ggcatgctcg ctgaggatgc
     7861 cgaacgcgta gcgcggctgc gtcgcgatcc gttgcgtcag ccgcccctcg tcgacccgca
     7921 ccgcgttcca gccggcctcg aacgtcaccg gcacgccgca gatccgctca taatcccgcc
     7981 agtaagcggg ggcggagtgg ctgacgtgca tctcctgcgc cagcgccccg tccgagaagg
     8041 ggcgggtcat gctgatgaag cgggcgaacg tggcctcggt gagctcggga aaggcgttcg
     8101 ggtccggccg cgtgtcgacc atccagacgc ggccctcgcg ccgctcgaac tggaacctcg
     8161 gcgccgcccc gacgccggtc tcgacgatca actggccgta gcggttcacc tgctcgatcg
     8221 actcggcgat cgtctcgcac gtgtagaaga tgaggccaac caccgaaaaa gccgacatgt
     8281 gcacggtggc gccgtagcga agcgccagcg ccgggtcgcc ggtcagcgtc ttggcgctcc
     8341 gcatcagcgc gacatattgg tccagcggga tacgcgcgtc ctgatcgtcg agcgcggcca
     8401 gcgcgattcc ggaccgctcg gccagctccg cggcgctcgc gcccgccgcg accgcaaccc
     8461 cgatcagccc tttggcgagc ccggcggcga cggtcagctc aggcaatgcg cggctccttt
     8521 gcaacctggg atcacgcttt tgattccggg aaccatatcc cggcactgtg ttgtcgttct
     8581 aacactccag ggtctcgggc tccaatcggg gagactctta cgcgatgacg ctgatcgtgc
     8641 acatcctgtt cggcagcgcc gctctggccg ccggcgccgc cggattgtgg ttccgcaagg
     8701 gttcgcgcgc ccacgccgcc gccggcacca ttttcttcgt cgccatgctg gtgatggccg
     8761 gaacgggcgc gctggtggcc gcgctcggtt cggaacgggg caccgccgtg atcggcctgt
     8821 tcaccctcta tctggtggcg acctcctggg ccaccgcccg gcggagggac ggaaccgccg
     8881 gtcggttcga ggtggcgggg ctgctggtcg ccagcgcctg cgccgccgct ttcaccgcct
     8941 gtgccgtcgt cgcggcgaac agcgcgtccg ggcgcctcga ttcgctgccg gcggcggccc
     9001 actacccgtt cgccatcgtc gcagcgctgg cggcgggcct ggacctcaac ttcatcctcc
     9061 gccgccggct cctcccccgg cagcggctgg cgcggcacct ctggcggatg tgcgccgcct
     9121 tcctgatcgc ggctttctcc ttcttcatgg gccagcagaa ggtgatgccc gatttcatgc
     9181 gcggctcgct tgctctgttc ctccccgaaa tcgccatcct cggcgcgatg acctactggc
     9241 tggtccgcct ccggctcggc aagaaatacg ctcgactggt gccggcgcgg gccgccgcgt
     9301 ccccgggcca gccgctcgcg ccggcgaagc ccgcatgagc ggcgctaccc cggatccgcg
     9361 gcacggctcg gcgcggctca ccggcctcct ctacgtgctc agcaacatca ccgcgatctt
     9421 cgccttctgg gtgcgcagcc ggctggtggc cggcaacgac ccggcccgaa ccgccgacaa
     9481 tatcatggcg tcggagccgc tgttccgggc gggcctcgcg agcgagctcg tcaccatcgt
     9541 cttgaccacg gctttggtgc tcgggctcta cgtcgtgctg aagccggtcg gccgcaatct
     9601 ggcctcgctc gccctgctct ggcgcatcct cgaaaattcg gtgctggcgg cgctcacctt
     9661 cgccagcttc accgcgctcg ccttgctcgg cggcggcgcc tatctcgcct cagtcgacac
     9721 cgcccaggcc gagggcctcg cctacgcgct gatccgggtc cacatatacg gcttccaggc
     9781 cggcttcctg ttcctggggc tgggctcggc tctgttcagc tatctgtggc tgaagtcgcg
     9841 ctacataccg ccgctgatcg ccgcctgggg catcttcgcc tcgttgctga tggcgtcggt
     9901 gtcgctggcg ctgatcgtga tgccgggcct gatctcggtc atcaccttgg cctatatgct
     9961 gccgatgggc ctttacgaga tcggcctcgg cctctggctg gtcgccaagg gcatccgctt
    10021 gcccgaacag gcgcgcccat cagccgacga ggcggaatag cggccggccg ggcttcacat
    10081 ccgggccaaa aacctgtaga acgaaaatcg aacaccggcc aaatcggaac cgcgcccggc
    10141 aagccgggat tcctgtgctg cgatgcaacg gtgaaaaaaa attttagcat gttgacagca
    10201 acgaagacgg ctcttttccg gcgcgtcgcg ggccccgccc ggcccgcatt gcggtgcggc
    10261 cggcgagccc ccatcttgcc aagtagcggc accccatatt agccctgtcg ccacaacaag
    10321 tagcagccca ccggcagacc gaccgctagg gcttgtgtat aaaaggggga gaaagctgtg
    10381 gattttgcag tgagcgagag cagccagctg gacgaaagcc gggccgagag cgacagcaaa
    10441 tcggtagcgc cgatccgctt caaggtgaag accgatcaga gccgcgacgc gctcctgacc
    10501 gagttcggca aggatacgct gcgcgaccgc tatctgctgc cgggcgagac ctatcaggac
    10561 ctcttcgccc gcgtcgcctc ggcctatgcc gacgacgccg cgcacgccca gcgcgtctac
    10621 gattacatct cccgcctctg gttcatgccg gcgaccccgg tgctctccaa cggcggcacc
    10681 ggccgcggcc tcccgatcag ctgctatttg aacagcgtcg acgacagctt gaacggcatc
    10741 gtcaacacct ggaacgagaa tgtctggctg gccgcgcgcg gcggcggcat cggcacttat
    10801 tggggcgcgg tccgcggcat cggcgagccg gtcggcctca atggcaagac cagcggcatc
    10861 atcccgttcg tccgcgtcat ggattcgctc accctcgcga tcagccaggg ctcgctccgc
    10921 cgcggctcgg ccgcctgcta tctcgacatc aaccacccgg aaatcgagga attcctcgaa
    10981 atccgcaaac cctcgggcga tttcaaccgc aaggcgctga acctccatca cggcgtgctc
    11041 gtcaccgacg ctttcatgga ggcggtgcgc gacggcgacg aatggatcct gcgcagcccc
    11101 aaggacggtt ccgagcgcgc ccgcgtcgac gcccgcgccc tgttccagaa gctggtcgag
    11161 accaggctgg cgaccggcga gccctacatc atcttcatcg acaaggtgaa ccgctcgatg
    11221 ccgcgccacc agcgcgagct cggcctcaag gtctcgacct ccaacctgtg ctcggagatc
    11281 acgctcccca ccggccgcga ccatctcggc aacgaccgca ctgcggtctg ctgcctctcc
    11341 tcgctgaacc tcgaaacctg ggacgagtgg aacaaggaca aggtcttcat cgaggacgtg
    11401 atgcgtttcc tcgacaacgt cctccaggat tatatcgacc gcgcccccgc cgagatggcc
    11461 cgcgccaagt acagcgcgat gcgcgagcgc tcggtcggcc tcggcgtgat gggcttccac
    11521 tccttcctcc aggcgcgcaa tatcgccttc gaaggcgcga tggccaaatc gtggaacctc
    11581 aagatcttcc gccacatctc ggcgcaggtc aacgaagcct cgatgatgct cgccaacgag
    11641 cgcgggccct gccccgacgc cgccgaccag ggcgtgatgg agcggttcag ctgcaagatg
    11701 gcgatcgcgc cgaccgcgtc catctcgatc atctgcggcg gcacctcggc ctgcatcgag
    11761 ccgatcccgg gcaacgtcta cacccacaag acgctgtccg gctccttctc gatccgcaac
    11821 ccgcatctcg agcggctgct gatcgccaag gccaagaatt ccgaagcggt gtggaattcg
    11881 attttggaga agaacggctc ggtccagcat ctcgatttcc tcacggctga ggaaaaggac
    11941 acgttcaaga ccagcttcga gatcgaccag cgctggctcc tggagctggc cggcgaccgt
    12001 acgccctata tcgaccaggc gacctcgctc aatctgttca tccccgccga cgtcgacaaa
    12061 tgggacctgc tcatgctcca ctatcgcgcc tgggagctcg gcatcaaaag cctctattat
    12121 ctccgctcca aatcggtcca gcgcgctggc ttcgcgggcg gcgtcgaggc cgacaacacg
    12181 ccggacctca agcagatcga gctccagacg acggactatg acgagtgttt agcctgccag
    12241 tgaggtgcgg gcggcccggc ttgcctccgg caagacgggg actcgccacg gcgccggcga
    12301 tgaccaatta gaatttcagg agaccaagat gtccctcacc gaagcccgca agcagtacaa
    12361 gcccttcgaa tatccctggg cgttcgactt ctggaagcgc cagcagcaga ttcactggat
    12421 gccggaggag gtccccctcg gcgaggattg ccgcgactgg gcgcagaagc tcaccgagca
    12481 cgagcgctct ttgctcaccc agatcttccg cttcttcacc caggccgacg tcgaggtgca
    12541 ggattgctac cacgacaaat atggccgcgt gttcaagccg accgagatca agatgatgct
    12601 gaccgccttc tccaacatgg agacggtcca catcgccgcc tacagccacc tgctcgacac
    12661 gatcggcatg cccgagagcg aatattcggc cttcctccaa tataaggaga tgaaggacaa
    12721 gcacgactat ctcgccacct tcggcgtcga caccgacgag gacatcgcca agacgctcgc
    12781 catgttcggc ggcttcaccg aggggctgca gct
//
LOCUS       DASVRK010000025         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_128872, whole genome shotgun sequence.
ACCESSION   DASVRK010000025 DASVRK010000000
VERSION     DASVRK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128872"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..682
                     /locus_tag="VF589_00955"
     CDS             167..682
                     /locus_tag="VF589_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256172.1"
                     /translation="MQRPSPIANAALAFVLLSVPAALAASPRSVRKLDAEAIQKVSER
                     KLCDAYGVWLSQGKQYPVIDGELRRRGIDCADAVDRLVSNCSVLEVVEVENPFPGGMV
                     YTVRNLSAKPKSFRIYDGGMVSSRFSIGAGSTQGFGVRSDPRIAGLGGVAAKLEGQTG
                     VELTECVTSSW"
     gene            735..>2446
                     /locus_tag="VF589_00960"
     CDS             735..>2446
                     /locus_tag="VF589_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8256173.1"
                     /translation="MAAPVLAFENLGLVQGSGWLFRGLDLFVGERDRLALIGRNGAGK
                     TTLLKCLAGLIETDEGRRTIRPGTRVVLLEQDPKVDRFATLREFALAAPDAPPAHAVD
                     AIASQIGIDLDRPAATASGGERRRAAIARALAMEPDVLLLDEPTNHLDLAAIEWLEEW
                     LGRYSGAFIAISHDRTFLKRLTRSSLWLERGSLRRAEIGFGGFDAWTEQVYAEEARAA
                     EKLDARLKLEFHWLERGVTARRKRNQGRLAKLGEMRAARAAMLGPAGTAKLGLQNDDV
                     KTKMVIDADKVTKRFGDRAVVRDFTLRIQRGDRIGVVGANGTGKTTLLRLLTGELEPD
                     EGKIRRAKTLQGIMIDQQRRLLDPAKSVRDVLADGGDWIDVRGHRKHVQGYLKEFLFE
                     PGLADAKIGTLSGGERSRLLLAREFARRSNLLVLDEPTNDLDLETLDLLQEVIADYDG
                     TVLIVSHDRDFLDRTVTITLGLDGSGKVDIVAGGYEDWERKRVAQRSVPAKVGTQTAG
                     ATAPRPAPASRTKLSYKDQRDYDLLPKRIEEIEASIARGERALSDPDLYARDPKRFAE
                     ISAG"
BASE COUNT          414 a          846 c          821 g          365 t
ORIGIN      
        1 gccggagctg gcggatgcgg cgccgatctg ccgctatgtg acaacggagc ttagcggcga
       61 cgagggcact ggagccatcc ccaaccagct ttactgccag acagccgaca atgcctggga
      121 gccctatgca ctcggcaagg cttgacgcac cgggaaagga aacagaatgc aacgtccctc
      181 gcccatcgcc aatgcggcgc tcgcgttcgt cttgctgtcc gtgccggccg cgcttgccgc
      241 atcgcccagg tccgtgcgca agctggatgc ggaggccatc cagaaggtat cggagcggaa
      301 gctgtgcgat gcttatgggg tgtggctatc gcaagggaag cagtatccgg tcatcgacgg
      361 cgaactgcgg cggcgcggca tcgactgcgc tgacgcggtc gaccggctcg tcagcaattg
      421 ttcggtgctg gaggtggtgg aggtcgaaaa ccccttcccg ggaggcatgg tctacacggt
      481 ccgaaacctg tcggcgaaac cgaagtcgtt ccgcatctac gatgggggga tggtttcttc
      541 gcgcttctcg atcggcgccg gttccaccca gggattcggc gtccgatccg atccccgcat
      601 cgccggcctt ggcggcgttg cggcgaagct ggagggccaa acgggggtcg aacttaccga
      661 atgcgtgaca tcgagctggt gagatccgct tcgatgtggt tgagccgcga cagccgggcg
      721 cctatctgcc cgccatggct gcacccgttc tcgcttttga aaatcttggc ctcgtccagg
      781 gctccggctg gctgttccgc gggctcgacc tgttcgtggg cgagcgcgac cggctggcgc
      841 tgatcgggcg caacggcgcc ggcaagacca cgttgctgaa atgcctcgcc gggctgatcg
      901 agaccgacga gggccggcgc acgatccggc ccggcacccg cgtcgtcctg ctcgagcagg
      961 atccgaaggt cgaccggttc gccacgctgc gcgagttcgc gctggccgcc cccgatgctc
     1021 cgccggcgca cgcggtcgac gcgatcgcca gccagatcgg catcgatctc gatcgccccg
     1081 ccgccaccgc ctcgggcggc gagcggcgcc gcgccgcgat cgcccgcgcg ctggcgatgg
     1141 agcccgacgt gctgctgctc gacgagccca ccaaccatct cgatctcgct gccatcgagt
     1201 ggctcgaaga atggctcggc cgctatagcg gcgccttcat cgcgatcagc cacgaccgca
     1261 cctttttaaa gcggctgacc cggtcgagcc tgtggctgga gcgcggctcg ctgcgccggg
     1321 ccgagatcgg cttcggcggg ttcgacgcct ggaccgagca ggtctatgcc gaggaagcgc
     1381 gcgccgccga gaagctcgac gccaggctca agctcgaatt ccactggctg gagcgcggcg
     1441 tcaccgcgcg gcgcaagcgc aaccagggcc ggctcgccaa attgggcgag atgcgcgccg
     1501 cgcgggcggc gatgctcggc ccggccggca ccgccaagct cggcctccag aatgacgatg
     1561 tgaagaccaa gatggtgatc gatgccgaca aggtcaccaa acgcttcggc gaccgcgccg
     1621 tcgtccggga tttcaccctg cgcatccagc gcggcgaccg gatcggcgtc gtcggcgcca
     1681 acggcaccgg caagaccacc ctgctgaggc tgctcaccgg cgaactggag cccgacgagg
     1741 gcaagatccg ccgcgccaag acgctccagg gcatcatgat cgaccagcag cgccggctgc
     1801 tcgacccggc caagagcgtc cgcgacgtgc tcgccgacgg cggcgactgg atcgacgtgc
     1861 gcggccaccg caagcacgtc cagggctatc tcaaggaatt cctgttcgag ccggggctcg
     1921 ccgacgcgaa gatcggcacg ctttcgggcg gcgagcgctc gcggctgctg ctcgcccgcg
     1981 aattcgcgcg gcgctcgaac ctgctggtgc tcgacgagcc gaccaacgat ctcgatctcg
     2041 agactctgga cctgctccag gaagtgatcg ccgattacga tggcaccgtc ctcatcgtca
     2101 gccacgaccg cgacttcctc gaccgcaccg tcaccatcac cctcggcctc gacggatcgg
     2161 gcaaggtcga catcgtcgcc ggcggctatg aggattggga gcgtaaacgc gtcgcgcagc
     2221 ggagcgtccc ggcgaaggtc gggacccaga cggcgggtgc aaccgccccc cgcccggcgc
     2281 ccgccagcag gaccaagctc agctacaagg accagcgcga ctacgacctg ctgccgaaac
     2341 ggatcgagga gatcgaggcg tcgatcgccc gcggcgagcg cgcgctgtcg gaccccgacc
     2401 tctacgcgcg cgacccgaaa cgcttcgccg aaatcagcgc ggggat
//
LOCUS       DASVRK010000026         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_66741, whole genome shotgun sequence.
ACCESSION   DASVRK010000026 DASVRK010000000
VERSION     DASVRK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66741"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VF589_00965"
     CDS             <1..1150
                     /locus_tag="VF589_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX8256174.1"
                     /translation="WPLWSEGSYSWYCNTIPTPDGGTHEAGLRAAITKGIRAFGELIG
                     QKKAKDITADDIVTGSEAMLSVFIRDPQFQSQTKDRLTSPDAARLVETAIRDHFDHFL
                     ADNMDRGRALLGFVLDRMDERLKRRAEREVKRKTATSARKLRLPGKLTDCANDDPAGT
                     ELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASATADKIRANSEIADLIQAMGC
                     GTRDKCHIDSLRYERIVIMTDADVDGAHIATLLMTFFFQEMPELVRKGALYLAQPPLY
                     RLAAGGTIAYAKDDAHRAELERTMFKGKKVEVSRFKGLGEMNPQQLRETTMDPKTRSL
                     IRITLPQQYEERAAIKDLVDRLMGKNPEHRFAFIQSHAAELDEEEIDA"
     gene            1158..1454
                     /locus_tag="VF589_00970"
     CDS             1158..1454
                     /locus_tag="VF589_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256175.1"
                     /translation="MLGRWTLRRAAGAGVVSGLAAVALWPAYAMLQEPLRLPYAAALA
                     AAAFCGLSILWITAKDVARGRRRGGKAKALRAFDVAFALLLTIPSLTALHALLS"
     gene            complement(1784..>2452)
                     /locus_tag="VF589_00975"
     CDS             complement(1784..>2452)
                     /locus_tag="VF589_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256176.1"
                     /translation="SDAGVAVRLANGAVSGGHAAGDSFAGFEDISGSAFDDRLSGDGG
                     VNALRGLGGNDILDGAAGNDTLDGGEGNDTARGGGGVDSLLGGDGNDRLEGGDDGDLL
                     TGGAGIDTLAGGGGRDILYFDDGDASAVRTQADRVVGHSRADLDRINLSRIDADTTEG
                     ADGDQAFTWLGAGAFSGAAGELRYQAINGDAYLQGDTDGDGIANFFIRVDDFTTLMRG
                     DLVV"
BASE COUNT          385 a          884 c          832 g          351 t
ORIGIN      
        1 ctggccgctg tggagcgaag gctcgtacag ctggtattgc aacacaatcc cgacgccgga
       61 cggcggcacg catgaggctg gactgcgggc cgcgatcacc aaaggcattc gcgcgttcgg
      121 cgagttgatc ggacaaaaga aagccaagga tatcacggcg gacgatatcg tcaccggcag
      181 cgaagccatg ctttcggtct tcatccgcga cccccagttc cagagccaga ccaaggaccg
      241 gctgaccagc cccgacgccg cccggctggt ggagaccgcg atccgcgacc atttcgacca
      301 tttcctcgcc gacaacatgg accgggggcg cgccctgctc ggcttcgtgc tggaccgcat
      361 ggacgagcgg ctgaagcggc gggccgagcg cgaggtcaag cgcaagaccg ccaccagcgc
      421 gcgcaagctc cgcctgcccg gcaagctcac cgactgcgcc aacgacgatc ccgccggcac
      481 cgaattgttc atcgtcgagg gcgacagcgc cggcggctcg gccaagcagg cgcgcgaccg
      541 caagacccag gcgattctgc cgatccgcgg caagatcttg aacgtcgcct ccgccaccgc
      601 cgacaagatc cgcgccaatt cggagattgc cgacctcatc caggcgatgg gctgcggcac
      661 gcgcgacaaa tgccatatcg acagcctgcg ctatgagcgc atcgtgatca tgaccgacgc
      721 cgacgtcgac ggcgcccaca tcgccacctt gctgatgacc ttcttcttcc aggaaatgcc
      781 cgagctggtc cgcaagggcg cgctctacct cgcgcagccg ccgctctacc gcctcgccgc
      841 cggcggcacc atcgcttacg ccaaggacga cgcccaccgc gccgagctcg aacggactat
      901 gttcaagggc aagaaggtcg aggtcagccg cttcaagggg ctcggcgaga tgaacccgca
      961 gcagctgcgc gagacgacga tggacccgaa gacgcgctcg ctgatccgga tcaccttgcc
     1021 gcagcaatat gaggaacggg cggcgatcaa ggatctggtc gaccggctga tgggcaagaa
     1081 tccagagcac cgcttcgcct tcatccagag ccacgccgcc gagctcgacg aggaggagat
     1141 cgacgcctga gcggccgatg ctgggtcgct ggaccctccg ccgcgcggcc ggcgcgggcg
     1201 tcgtcagcgg gctcgccgcg gtcgcgctgt ggccggctta tgcgatgctg caggagccgc
     1261 tccgcctgcc ttatgcggcg gcgctggcgg cggcggcctt ttgcggcctc tctatcctgt
     1321 ggatcacggc caaggacgtg gcgcgcgggc gccgccgcgg cggcaaggcc aaggcgctcc
     1381 gcgccttcga cgtcgccttc gctctgctcc tcaccatccc cagcctcacc gcgctccacg
     1441 ccctcctgag ctgagccggg cccctgcctt cgcagggggc acgtggcgca tgcttggggt
     1501 ttgcagtgcc cctgcgaagg caggggtcca ggccgggcgc agcccgccaa ccaagctctc
     1561 ggaaacatcg cgaggggccg ggaccgtgtg cggtgccaag ccttcacggc cgctgcgatg
     1621 accggactgc cgctgaagcg gcagcccggg tctgggcccc tgccttcgca ggggcacgtg
     1681 ctggggcttt ccgttcttag tgcccctgcg aaggcagggg cccaggccgg gcgcagcccg
     1741 ccagccaggc tctcgtaaat atcgcggcag cccgggaccg ggctcagacc acgagatcac
     1801 cgcgcatcag ggtggtgaag tcgtccaccc ggatgaagaa gttggcgatt ccgtcgccgt
     1861 ccgtgtcgcc ctgaaggtac gcgtcgccgt tgatcgcctg gtagcgaagc tccccggccg
     1921 ccccgctgaa cgcgccggcg ccgagccagg tgaaggcctg gtcgccgtcc gcgccttcgg
     1981 tggtgtcggc gtcgatccgg ctgaggttga tgcgatcgag gtcggcgcgg ctgtgcccga
     2041 ccacgcggtc ggcctgggtg cgcacggcgg aagcgtcgcc atcgtcgaaa tagaggatgt
     2101 cgcgcccgcc gccgccggcc agggtgtcga tgccggcgcc gcccgtgagc aggtcgccgt
     2161 cgtcgccgcc ctcgagccgg tcgttgccgt cgccgccgag caggctgtcg acgccgccgc
     2221 cgccgcgcgc ggtgtcgttg ccctcgccgc cgtcgagcgt gtcgttgccg gccgcgccat
     2281 cgagtatgtc gttgccgccg aggccgcgca gcgcgttgac gccgccgtcg cccgacagcc
     2341 ggtcgtcgaa ggccgagccg gagatgtcct cgaagccggc gaagctgtcg ccggcggcgt
     2401 ggccgcccga gaccgcgccg ttggccagcc gcaccgccac gcccgcgtcc ga
//
LOCUS       DASVRK010000027         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234 k141_4238,
            whole genome shotgun sequence.
ACCESSION   DASVRK010000027 DASVRK010000000
VERSION     DASVRK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4238"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VF589_00980"
     CDS             complement(<1..778)
                     /locus_tag="VF589_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX8256177.1"
                     /translation="MTDTAAAAPQVAAEEPAGGHAPQGNVAKLAVGAIGIVFGDIGTS
                     PIYAFRETFAGAHHSLTLDRLHIFGVLSLIFWSMMVVVTFKYVTILMRADNKGEGGSL
                     ALLALINRRSEKKRRWSAGIVLLGVFATALFYGDSMITPAVSVLSAVEGLTVVDPRFE
                     YLVIPLAIGILIGLFAIQARGTARVGALFGPIMLVYFVTLMVLGLVNIVAQPGVLWAL
                     NPYYAFQFFYTDKWLAFLALGSVVLAVTGAEALYADMGHVG"
     gene            938..>1654
                     /locus_tag="VF589_00985"
     CDS             938..>1654
                     /locus_tag="VF589_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX8256178.1"
                     /translation="MRVGVPKEIKVHEYRVGLTPASVAELVAAGHEVIVETKAGTGID
                     LDDQAYIEAGARIAGSAAETFAAADMIVKVKEPQQQEIALLEARHLLFTYLHLAADKP
                     QAEGLMKSGATCIAYETVTANDRSLPLLKPMSEVAGRMSVQVGAHYLEKEQGGRGVLL
                     GGVPGVAPARVTILGGGVSGVNAAQMAVGMRADVTIYDINMERLAELDMFFSSQIKTA
                     YASRSAIAKAVEEAELVIGAV"
BASE COUNT          315 a          554 c          542 g          243 t
ORIGIN      
        1 ggccgacatg gcccatgtcc gcgtagagcg cttcggcccc ggtcaccgcc agcaccaccg
       61 agcccaaggc gaggaaggcg agccacttgt cggtgtagaa gaactggaag gcgtaatagg
      121 ggttgagcgc ccacagcacg cccggctgcg cgacgatgtt gacgaggccg agcaccatca
      181 aggtcacgaa atagacgagc atgatcggcc caaacagggc gccgacccgg gcggtgcccc
      241 gcgcctggat ggcgaacagg ccaatgagga tgccgatcgc gagcgggatc acgagatatt
      301 cgaagcgggg gtcgaccacc gtcagcccct ccaccgccga cagcaccgag acggccggcg
      361 tgatcatgct gtcgccgtag aacaaggcgg tggcgaagac gccgagcagc acgatgccgg
      421 cgctccaccg ccgcttcttc tccgatcggc ggttgatcag cgccagcaag gcgaggctgc
      481 cgccctcccc cttattgtcc gcccgcatca ggatggtgac atatttgaac gtcaccacca
      541 ccatcatcga ccagaagatc agcgacagca cgccgaagat gtgcagccgg tcgagcgtca
      601 gcgaatgatg cgcgccggcg aaggtctcgc ggaaggcgta gatcgggctg gtgccgatgt
      661 cgccgaagac gatgccgatc gcgccgaccg caagtttggc gacattgccc tgcggggcat
      721 ggccgcccgc aggctcctct gcagcgacct gcggtgcggc ggcggcggtg tcggtcattg
      781 gacaggcagg cggaacgccc tgaaaccttt cgctcaaagc tctggacccg ccaggcgctg
      841 ccgcgcttta gcatgtcgcc gccgagcttc gcaacggcga caaatgcttt cgcggccgct
      901 atagcgcgcg ccacaacccg ttctgatgga gacgaagatg cgcgtcggcg tgcccaaaga
      961 gatcaaagtt cacgaatatc gcgtcggcct cacccccgcc tcggtcgcgg aactggtggc
     1021 ggccggccat gaggtgatag tcgagaccaa ggccggcacc ggcatcgacc tcgacgacca
     1081 ggcctatatc gaggccggcg cgcggatcgc gggcagcgcc gccgagacct tcgccgccgc
     1141 cgacatgatc gtgaaggtca aggagccgca gcagcaggag atcgccctgc tcgaggcccg
     1201 ccacctgctg ttcacctatc tccacctcgc agccgacaag ccgcaggccg agggcctgat
     1261 gaagtccggc gcgacctgca tcgcctatga gacggtcacc gccaacgacc gctcgctgcc
     1321 gctcttgaag ccgatgtcgg aagtcgccgg ccgcatgtcg gtccaggtcg gcgcccatta
     1381 tctcgaaaag gagcaaggcg gccgcggcgt gctgctcggc ggcgtccccg gagtcgcgcc
     1441 ggcgcgcgtc accatcctcg gcggcggcgt ctccggcgtc aacgcggcgc agatggcggt
     1501 cggcatgcgt gccgacgtca ccatctatga catcaacatg gagcgtctgg cggagctcga
     1561 catgttcttt tccagccaga tcaagaccgc ttatgcgagc cgctcggcga tcgccaaggc
     1621 tgtcgaggaa gccgaacttg tgatcggcgc ggtt
//
LOCUS       DASVRK010000028         4221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_315882, whole genome shotgun sequence.
ACCESSION   DASVRK010000028 DASVRK010000000
VERSION     DASVRK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315882"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2208
                     /locus_tag="VF589_00990"
     CDS             <1..2208
                     /locus_tag="VF589_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HEX8256179.1"
                     /translation="QAYHSPGRTGGEKPGVYGKLLAAIAAESGIELRVLADILAGSSA
                     GGINAIFLAEAISSGQSLDPLTELWLDAADVDSLLAPGTSRMSSFAKKAAVPVAWALS
                     SRRGGAVEQTVEPGHREEVRAKLAHFVRARWFAPPFGGPEFTGLLLDAFEAMKQGPAG
                     PPLLPEHQPLDLFVTVTDFHGHPERLRLHSPPEVAETEHRITLAFRDPGGRSRHLADA
                     AELAFAARATASFPGAFPPFRVGELDAVLESRDQPWPERDAFLKRALPRQWAAGEAEN
                     AVLIDGSVLANAPFRPAIEALRSRPARREVDRRFVYIDPKPGIHSIRLRRAGEAETPG
                     FLATILGALSDLPREQPIRDNLEAIEGRSARIRRLRHILAGMRPQVESAIERTFGTTL
                     LLGRPTAARLAAWRLRAHKVASREAGFAYVGYGHLKIATIVEELAASAHRLGGGGGRA
                     RLDRVREAIWERVRAAGLIGAEALTERGARPDVAEFLRNFDLSFRTRRLRFVAWRLAE
                     LDQQPDPPRAAIEAAREAVYDLIGRYRAPLESESDTAVPVFEAIDRDPAAGLAALAAR
                     LDLKSRDDEADAVFADVLAAFPKLERRALLLSYLGFPFFDIATLAMLQGEGLDEFDPV
                     KVDRISPDDATAIRKGGTAATLKGIQFNSFGAFFSRAYRENDYLWGRLHGADRLIDIV
                     VSTLPEDSGLAAATVAALKREAFEAILAEERPRLGSIGPLFDALDAEIEQAFR"
     gene            2260..2613
                     /locus_tag="VF589_00995"
     CDS             2260..2613
                     /locus_tag="VF589_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX8256180.1"
                     /translation="MQFLKTLFWVALAVILVLFANANWKSVTITLWGGLEADVKLPIL
                     VLFSFLVGFLPMLILHRARLWSLKRRLEAQERQVGAVHVPAAAAVPPPAPPPPSPSAP
                     EDERIATDSKAWPAT"
     gene            2610..3284
                     /gene="pyrF"
                     /locus_tag="VF589_01000"
     CDS             2610..3284
                     /gene="pyrF"
                     /locus_tag="VF589_01000"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX8256181.1"
                     /translation="MTSPVYVAIDTPDLDRAKAIAARVRNHVGGLKLGLEFFAANGRH
                     GIREMAELGLPIFLDLKLHDIPNTVGKAIQALRPLEPAILTVHGAGGRAMLEDAKAAA
                     PLGTKVVAVTVLTSLDTDDLAAIGIGGDPHAQVERLTGLAREAGLDGVVCSGNEVAAA
                     KRLWPDGFFVVPGVRPSNGNVGDQKRVMTPRAALDAGASILVVGRPITQAEDPDEAAR
                     AIGATL"
     gene            3346..4197
                     /gene="accD"
                     /locus_tag="VF589_01005"
     CDS             3346..4197
                     /gene="accD"
                     /locus_tag="VF589_01005"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX8256182.1"
                     /translation="MSWLNRVRQKIPFLPKRETPDNLWHKCRGCDSMIFTKEWQENLF
                     VCPRCEHHDRIGPRQRFEQIFDPGFTILPPPPVREDPLRFRDSKRYSDRIKSARQQTG
                     EPDALVNARGTIEEQRAIVGVQDFAFMGGSMGLGVGAAFVAGARAAIEDRCPYVIFTA
                     AGGARMQEGILSLMQMPKTTVAIAELKEAGLPYIVVLTDPTTGGVTASYAMLGDVQIA
                     EPGALIGFAGQRVIESTIREKLPEGFQRAEYLHDHGMVDMVVHRKDLRQRLAQLIAYL
                     APQRRAA"
BASE COUNT          612 a         1512 c         1459 g          638 t
ORIGIN      
        1 caggcctatc attcgcccgg gagaacgggc ggcgaaaagc ccggagtcta tggcaaattg
       61 ctggcggcga ttgcggccga gagcggaatc gagcttcgcg tgctcgccga catactcgcc
      121 ggctccagcg cggggggaat caacgccatc ttcctcgccg aggcgatctc gtccggccaa
      181 tcgctcgacc cgctgaccga gctctggctc gatgccgccg acgtcgatag cctgctcgcc
      241 cccggcacca gccggatgtc gagcttcgcc aagaaggctg cggtgccggt ggcctgggcg
      301 ctgtcctcgc gccgcggcgg cgccgtcgag cagaccgtcg agcccgggca tcgcgaggag
      361 gtccgcgcca agctcgccca tttcgtgcgg gcgcgctggt tcgcgccgcc gttcggcgga
      421 cccgagttca ccgggctgct gctcgacgcg ttcgaggcga tgaagcaggg gccggccggc
      481 ccgcccctgc tgcccgagca ccagccgctc gacctgttcg tcactgtcac cgatttccac
      541 ggccatcccg agcggctgcg gctccattcg ccgcccgaag tggccgagac cgagcatcgc
      601 atcaccctcg cctttcgcga ccccggcggg cggagccgcc acctcgccga cgccgccgag
      661 ctcgcctttg ctgcgcgcgc gacggcgagc ttcccgggcg ccttcccgcc gttccgggtc
      721 ggcgagctcg acgcggtgct tgaaagtcgc gaccagccct ggccggagcg cgacgctttc
      781 ttaaagcgcg ctttgccgcg gcaatgggcg gcgggcgagg ccgagaatgc ggtgctgatc
      841 gacggttcgg tgctcgccaa cgcgccgttc cggccggcga tcgaggcgct gcgcagccgg
      901 cccgcgcgcc gcgaggtcga ccgccgcttc gtctatatcg accccaagcc cggcatccac
      961 agcatccgcc tccgccgcgc cggcgaggcc gagacgcccg gcttcctcgc caccatattg
     1021 ggcgcgcttt cggacctgcc gcgcgagcag ccgatccgcg acaatctcga agcgatcgag
     1081 ggccgctcgg cgcggatccg caggctgcgc cacatcctcg ccggcatgcg gccgcaagtg
     1141 gagagcgcga tcgagcgcac gttcggcacg acgttgctgc tcgggcggcc gacggcggcg
     1201 cggctggcgg cgtggcggct gcgcgcgcac aaggtcgcct cgcgcgaagc gggcttcgct
     1261 tatgtcggct acggccattt gaagatcgcc accattgtcg aggagctggc ggcgagcgcg
     1321 caccgcctcg gcggcggcgg cggacgggcg cggctggacc gggtgcgcga ggcgatctgg
     1381 gaacgggtgc gggcggcggg gctgatcggg gccgaagcgc tgaccgagcg cggcgcgcgg
     1441 cccgacgtcg ccgaattcct gcgcaatttc gacctctcct tccgcacccg gcggctgcgc
     1501 ttcgtcgcct ggcggctggc cgagctcgac cagcagcccg atccgccgcg ggcggcgatc
     1561 gaggcggcgc gcgaggcggt ctacgacttg atcggccgct accgcgcccc gctcgaaagc
     1621 gagagcgaca cggccgtgcc ggtgttcgag gcgatcgacc gcgatccggc cgccggcctc
     1681 gccgcgctcg ccgcacggct ggacctcaag agccgcgacg acgaggcgga cgccgtgttc
     1741 gcggacgtgc ttgccgcctt tcccaagctc gagcggcggg cgctgttgct ctcctatctc
     1801 ggcttcccct ttttcgacat cgccacgctc gcgatgctgc agggcgaggg gctcgacgag
     1861 ttcgatccgg tcaaggtcga ccgcatctcg cccgacgacg cgaccgcgat ccgtaagggc
     1921 ggcacggcgg cgacattgaa ggggatccag ttcaacagct tcggcgcctt tttcagccgc
     1981 gcctatcgcg agaacgacta tctgtggggc cgcctccacg gcgccgaccg cctgatcgac
     2041 atcgtcgtct cgacattgcc cgaagattcg gggcttgccg ccgcgacggt ggcggcgctg
     2101 aagcgcgagg cgttcgaggc gatcctggcc gaggagcggc cgcggctggg ctcgatcggg
     2161 ccgctgttcg atgcgctgga cgcagagatc gagcaggctt tccgctagac tggccggcga
     2221 ttttgttaga gcgtaagcgg cggcggtcgg gagagttgga tgcagttcct caagactttg
     2281 ttctgggtcg cgctggcggt gatcctggtg ctgttcgcca acgccaattg gaagtcggtg
     2341 acgatcacgc tgtggggtgg gctcgaggcc gacgtgaagc tgccgatcct ggtgctgttc
     2401 tccttcctgg tcggcttcct gccgatgctg atcctccacc gcgcgcggct ctggtcgctg
     2461 aagcgccggc tcgaggcgca ggagcggcag gtgggcgcgg tgcacgtccc ggccgccgcc
     2521 gcggtgccgc cgccggcgcc gccgccgccc tcgccctccg cgcccgagga cgaacgtatc
     2581 gccaccgaca gcaaggcctg gccggcgaca tgaccagccc ggtctacgtc gcgatcgaca
     2641 cgccggacct cgaccgcgcc aaggcgatcg cggcgcgcgt gcgcaaccat gtcggcggtc
     2701 tgaagctggg cctcgaattc ttcgcggcca acggccgcca cggcattcgc gagatggccg
     2761 agctcggcct gccgatcttc ctcgatctga agctccacga catccccaac acggtcggca
     2821 aggcgatcca ggcgctgcgc ccgctcgagc ccgcgatcct caccgtccac ggcgccggcg
     2881 gccgggcgat gctcgaagac gccaaggccg cggcgccgct gggcaccaag gtggtggcgg
     2941 taaccgtgct caccagcctc gatacggacg acctcgccgc gatcggcatt ggcggcgacc
     3001 cgcacgcgca ggtcgagcgg ctgaccggcc tcgcccgcga agccgggctc gacggggtgg
     3061 tctgctccgg caatgaggtg gcggcggcca agcggctctg gcccgacggc ttcttcgtcg
     3121 tccccggcgt gcggccgtcc aacggaaacg tcggcgacca gaaacgggtg atgaccccgc
     3181 gcgcggcgct cgacgccggc gcctcgatcc tggtcgtcgg ccgcccgatc acccaggccg
     3241 aggatcccga cgaagccgcc cgcgcgatcg gcgccacgct ctagagccat tggtctagac
     3301 aaaatcaaag gcttgatcca agcggcgggc gaaggagatg cccgcatgag ctggctcaat
     3361 cgcgttcgcc agaagatccc gttcctcccc aagcgcgaga cgccggacaa tctctggcac
     3421 aaatgccgcg gctgcgattc gatgatcttc accaaggaat ggcaggagaa tctgttcgtc
     3481 tgcccgcgct gcgagcatca cgaccggatc ggcccgcgcc agcggttcga gcagattttc
     3541 gaccccggct tcaccatcct gccgccgccg ccggttcgcg aggacccgct acgcttccgc
     3601 gattcgaaac gctattcgga ccgcatcaag agcgcccgcc agcagaccgg cgagccggac
     3661 gccctcgtca acgcgcgcgg caccatcgag gagcagcgcg cgatcgtcgg cgtgcaggat
     3721 ttcgccttca tgggcggatc gatggggctc ggcgtcggcg ccgccttcgt cgccggcgcg
     3781 cgcgcggcga tcgaggatcg ctgcccctat gtgatcttca ccgcggccgg cggggcgcgc
     3841 atgcaggaag gcatcttgtc gctgatgcag atgcccaaga cgacggtcgc catcgctgag
     3901 ctcaaggaag cggggctgcc ttatatcgtg gtgctgaccg atcccaccac cggcggcgtc
     3961 accgcctcct atgcgatgct gggcgacgtc cagatcgccg agcccggggc gctgatcgga
     4021 ttcgccggcc agcgggtgat cgaatcgacc atccgcgaga agctgcccga aggcttccag
     4081 cgggccgaat atctgcacga tcacggcatg gtcgacatgg tggtgcaccg gaaggacctg
     4141 cgccagcggc tcgcccagct catcgcctat ctcgccccgc agcggcgggc ggcgtgacgc
     4201 accccctctc cccttcaggg g
//
LOCUS       DASVRK010000029         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_191623, whole genome shotgun sequence.
ACCESSION   DASVRK010000029 DASVRK010000000
VERSION     DASVRK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191623"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VF589_01010"
     CDS             <1..673
                     /locus_tag="VF589_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HEX8256183.1"
                     /translation="SPQVLRDGFTWFEQGEAEVEAFEDVRFPIAIGRAASVEPAFSTA
                     VVTTAGGAEQRNSDWADARMRFDAGPGVRGEAELQLLIAFFRARRGAAVAFRFEDPYD
                     HSSNGMTDEPAAADQPIGVGDGVRTEFALVKHYEAQMRRITRPVVGSVLVAVDGVERV
                     SGWTLAGLGLVRFEVAPAAGAEVRAGYRFDVPVRFAEDRLSLSRATFEAGEAASVALI
                     EVREA"
     gene            670..1485
                     /locus_tag="VF589_01015"
     CDS             670..1485
                     /locus_tag="VF589_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEX8256184.1"
                     /translation="MSIVNGEVTSLAFCWRLERRDGVCLGFTTHDRDLTIGELVYRAA
                     PGMLPSAIALSDGFDPDTLDVSGALTSAAITAEDLAAGRWDGAALAILAVDWEAPGGE
                     RMLLARGELGEVSVTGSGFSAELRGPTALLERPVVEQTSPECRAQLGDKRCRVDMAAR
                     VRVTRVVAVADEDELVVASAAAGNAYGYGRLRWLSGPNSGLESAVLMSDGTRLTLREP
                     PHFPAAAGDLVEIHEGCDKQPATCSSRFGNLPNFRGEPHLPGMDLLTRYPGAS"
     gene            1510..1908
                     /locus_tag="VF589_01020"
     CDS             1510..1908
                     /locus_tag="VF589_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HEX8256185.1"
                     /translation="MTEGHAVAMRARRLVGVRFRPQGRSVEHGLDCLGVAALAAGLPL
                     ELVPRRYRMSDEVSQALLGLDFGGRAKPLPPAEARVGDVLLVETGPRRRHFVVLVDGG
                     FVHADARLGRVSEVPGPVAWPLVAAWRVAG"
     gene            1934..>2642
                     /locus_tag="VF589_01025"
     CDS             1934..>2642
                     /locus_tag="VF589_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256186.1"
                     /translation="MATLVLTAVGTSVGGPVGGMIGALIGQQIDAQIFKPKGRQGPRL
                     GDLSVQTSSYGTAIPKLFGIMRVAGTVIWATDLQEHEDSSGGGKGQPKTTLYSYSASF
                     AVAISARPVRDVKRIWADGKLLPGAAGDFKSATGYRLYRGGEDQPPDPLIAAAEGATP
                     AFRGIAYALFEDFQLEDYGNRIPSLTFEVEADAGPVAIGEVAEDLSGGEVRDAGTPEV
                     AGYAASGDSIRAAIEALG"
BASE COUNT          356 a          780 c         1101 g          405 t
ORIGIN      
        1 ttcgccgcaa gtgctgcgcg acggcttcac atggttcgag caaggggagg cggaagtgga
       61 agctttcgag gacgtgcgct ttccgatcgc gatcgggcgg gcggcgagcg tcgagccggc
      121 tttttcgacg gcggtggtga cgacggcggg cggcgccgag cagcgcaaca gcgactgggc
      181 cgacgcgcgg atgcggttcg acgccggacc cggagtgagg ggcgaggccg agctccagct
      241 gttgatcgcc tttttccggg cgcggcgggg ggccgcggtc gcgttccggt tcgaggatcc
      301 gtacgatcac agctccaacg ggatgaccga cgagccggcc gccgccgacc agccgatcgg
      361 cgtcggcgac ggggtcagga ccgagttcgc gctggtcaaa cattatgaag cgcaaatgcg
      421 gcggatcacg cggccggtgg tggggagcgt gctcgtggcg gtcgatgggg tcgagcgggt
      481 gagcggctgg acgctggccg ggctgggtct ggtgcgtttc gaggtggcgc cggccgcggg
      541 cgccgaggtg cgggcgggct accggttcga cgtgccggtg cgcttcgccg aggaccggct
      601 gagcctcagc cgggcgactt tcgaagcggg cgaggcggcg agcgtcgccc tcattgaggt
      661 gcgcgaggca tgagcatcgt caacggcgag gtcaccagcc tcgctttctg ctggcggctg
      721 gagcggcggg acggggtgtg cctcggcttc accacgcacg atcgggatct cacgatcggg
      781 gagctcgtct atcgcgcggc gcccggcatg ctgccgtcgg cgatcgcgct gtcggacggg
      841 ttcgaccccg atacgctcga cgtgtcggga gcgcttacca gcgcggcaat cacggcggag
      901 gatttggcgg cggggcgctg ggacggcgcc gcgctggcga tcttggcggt cgactgggaa
      961 gcgccgggcg gggagcggat gctgctggcg cgcggcgaat tgggcgaggt cagcgtgacg
     1021 gggagcgggt tttcggcgga gctaaggggg ccgaccgcgc tgctcgagcg gccggtggtg
     1081 gagcaaactt cgcccgaatg ccgggcgcag cttggcgaca agaggtgccg ggtcgacatg
     1141 gccgcgcggg tgcgggtgac gcgggtggtg gcggtggcgg acgaggatga gctggtggtc
     1201 gcgagcgcgg cggcgggcaa tgcctatggc tatggccggc tgcgctggct cagcggaccg
     1261 aatagcgggc tggagagcgc ggtgctgatg tcggacggga cgcggctgac gctgcgcgag
     1321 ccgccgcatt ttccggcggc ggccggcgac ctcgtcgaga tccacgaagg ctgcgacaag
     1381 cagccggcga cttgctccag ccggttcggc aatttgccga actttcgggg cgagccgcat
     1441 ctgccaggca tggacctgct gacccgttat ccgggcgctt cctgacggcc acaatcatcg
     1501 gagcgggcga tgacggaggg gcatgcggtc gcgatgcggg cgcggcggct ggtgggcgtg
     1561 cgctttcggc cgcaggggcg aagcgtggag catgggctcg actgcctcgg cgtcgcagcg
     1621 ctcgcggccg ggctgccgct ggagctggtg ccgcgccgct accggatgag cgacgaggtt
     1681 tcgcaggcgc tgctcgggct cgatttcggg ggccgcgcga agcccttgcc gccggcggag
     1741 gcgagggtgg gggacgtgct gctggtcgag acggggccgc gccggcgtca tttcgtggtg
     1801 ctggttgatg gcgggttcgt gcacgccgat gcgcggctcg gccgggtgag cgaggtgccg
     1861 gggccggtgg cgtggccgct agtcgcggcg tggcgggtcg ccggctgagc ggcgcaatcg
     1921 ggaggcatta tatatggcga cgttggtgct gacggcggtc gggacctcgg tcggcggtcc
     1981 ggtaggcggc atgatcggcg cgctcattgg ccagcagatc gacgcgcaga tcttcaagcc
     2041 caaggggcgc caggggccgc ggctgggcga cctcagcgtc cagacctcgt cctacggaac
     2101 cgcgatcccg aagctgttcg gaatcatgcg ggtggcgggc acggtgatct gggcgaccga
     2161 ccttcaggag catgaggatt cgagcggggg cggcaagggc cagccgaaga cgacgctcta
     2221 cagctattcg gcctcgttcg cggtggcgat ctcggcccgg ccggtgcggg acgtgaagcg
     2281 gatctgggcg gacggaaagc tgctgccggg cgcggcgggc gacttcaaga gcgcgaccgg
     2341 ctaccggctc taccgcggcg gcgaggacca gccgcccgat cctttgatcg ccgccgccga
     2401 aggcgcgacg ccggcgtttc gcggcatcgc ttatgcgctg ttcgaggatt tccagctcga
     2461 ggattatggc aaccgcattc cctcgctgac cttcgaagtg gaggccgatg cgggtccggt
     2521 ggcgatcggg gaggtcgcgg aggatctgag cgggggcgag gtgcgggacg cgggcacgcc
     2581 cgaagtggcg ggctatgccg cctccggcga cagcatccgg gccgcgatcg aggcgctcgg
     2641 cg
//
LOCUS       DASVRK010000030         5143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_254168, whole genome shotgun sequence.
ACCESSION   DASVRK010000030 DASVRK010000000
VERSION     DASVRK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254168"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VF589_01030"
     CDS             <1..578
                     /locus_tag="VF589_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8256187.1"
                     /translation="AGLSFAVFVAMAMLGGPGWAVDQRWLAQLSVYEDTTFARNAVIL
                     TTMGGWEVLTGLTIAFAIGLTFRRKMRAALLLIIVFGGRMVVELLKMFIERPRPGQSP
                     YLEAVSSNSYPSGHAANAMITFLAIAFLVPVRQRNRAIAMGLGFAAALQVGWSRVALG
                     VHWPSDVIGGWAFGLLWIAICVKLATDRPGD"
     gene            645..905
                     /locus_tag="VF589_01035"
     CDS             645..905
                     /locus_tag="VF589_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256188.1"
                     /translation="MSNENDRSNERAETAKRHDDSDLIDSMEGAPSHGNRAGHRIGHE
                     IGSRDELKQEVGEGSVTRVRDSDKPEGANLPRFNQGNSKLNP"
     gene            complement(1351..1965)
                     /locus_tag="VF589_01040"
     CDS             complement(1351..1965)
                     /locus_tag="VF589_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX8256189.1"
                     /translation="MSDDRRSAPHVARNAGPIAVVLRGILPDAGLVLEVASGSGEHAL
                     HFAAAFPHLMWQPTDADPVGLRSIDAWRTAERLPNLLAPVPLDAAAGEWPVNEAAAIL
                     CINMVHISPWAATVGLMRGAARLLAPGAPLYLYGAYRRQDVPTAPSNEAFDRSLRDRN
                     PEWGVRDLEAVAAEAERQGLRLDSVIEMPANNLSVVFRRVSRSS"
     gene            complement(1962..3569)
                     /gene="gcvPB"
                     /locus_tag="VF589_01045"
     CDS             complement(1962..3569)
                     /gene="gcvPB"
                     /locus_tag="VF589_01045"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX8256190.1"
                     /translation="MSINASGWKPEIGTAERAEAVTFTGNRALMLEEPLIFELGEEDG
                     CGVDFDFAGPLHPSASPSGPPPRSGEDKLAGLQRHRPIGLPGLSEPQTVRHYTRLSRQ
                     NYAIDLGLFPLGSCTMKHNPRLNEKVARMPGFADIHPLQPADTVQGALKLIADLGEWL
                     IKLTGMHSVAMSPKAGAHGELCGLLAIRSALEARGDARQVVLVPESAHGTNPATAAFA
                     GYRVENIPANDRGRVDLDALKSRLGPDVAAVMITNPNTCGLFEPDMTAISEAVHAAGG
                     LVYCDGANFNAIVGRVRPGDLGIDAMHINLHKTFSTPHGGGGPGSGPVVFSEALTPFA
                     PLPFVEKQGEAYALIEEESAGEHHGSTFGRMTAFHGQMGMFTRALAYILSHGTDGLRQ
                     VSGDAVLNANYVLRQLEDVLDAPFGASGPCMHEALFSDKGFAGGLSTLDLAKGLIDEG
                     YHPMTMYFPLVVHGAMLVEPTETESKAALDQFIGALRSVAERARAGDQSLKAAPVHAP
                     RRRLDETQAARKPVLVYKEPDRAQAAE"
     gene            complement(3566..4924)
                     /gene="gcvPA"
                     /locus_tag="VF589_01050"
     CDS             complement(3566..4924)
                     /gene="gcvPA"
                     /locus_tag="VF589_01050"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX8256191.1"
                     /translation="MRYLPLAEADRQEMLRAVGAASVEELFVDVPEAARLAGKIEGLP
                     DHASELAVERQLGALARRNLVAGEVPFFIGAGAYRHHIPASVDHIIQRGEFLTSYTPY
                     QPEIAQGTLQALFEFQTQVARLYGCDVANASMYDGSTACWEAITMAGRVTRRGKAILS
                     SGLHPHYVSVAETMARFTGDRLDVAPPELAAATDAERLLAAIDGETSCVVVQYPDILG
                     RIADLTPIAEKAHANGALLVAVVTEPVALGAIRSPGEMGADIVVGEGQAIGVGLNFGG
                     PYVGLFACKEKFVRQMPGRLCGETVDAEGKRGFVLTLSTREQHIRREKATSNICTNSG
                     LCALAFTVHMTLLGEAGLRRLAEINHVKAVAAAERLARVPGVELVNEAFFNEFTLRLP
                     VEARPVVRILAERGMLGGVSLGRLYPGVEALRNGLVLAVTETVTDEDVEALAGALEEI
                     LA"
BASE COUNT          806 a         1769 c         1783 g          785 t
ORIGIN      
        1 ccgccggcct gagcttcgcg gtattcgtcg cgatggcgat gctcggcggc cccggctggg
       61 cggtcgacca gcgctggctc gcgcagctca gcgtgtatga agacacgacg ttcgcccgca
      121 acgcggtcat tctgaccacg atgggcggct gggaggtgct gaccggcctc accatcgcct
      181 tcgccatcgg cctcaccttc cggcgcaaga tgcgcgcggc gctgcttttg atcatcgtgt
      241 tcggcggccg catggtcgtc gagctgttga agatgttcat cgagcgccct cgcccgggcc
      301 agtcgcccta tctcgaggcg gtgtcgtcga acagctatcc gagcggccat gcggccaacg
      361 cgatgattac cttcctcgcc atcgccttcc tggtgccggt gcggcagcgc aaccgggcga
      421 tcgcgatggg gctcggcttc gcggccgcgc tgcaggtcgg ctggagccgg gtcgccctcg
      481 gcgtccactg gccgagcgac gtgatcggcg gctgggcgtt cggcctgctg tggatcgcga
      541 tctgcgtgaa gctcgccacc gaccggcccg gcgactgacc ggcgcggcga tccgaggaac
      601 gccgggccgc cctccttcat tgatgcggtg aaggagactt tacgatgtcc aacgagaatg
      661 accgctcgaa cgagcgcgcc gaaaccgcca agcgccacga cgacagcgac ctcatcgatt
      721 cgatggaagg ggcccccagc cacggcaacc gcgccggcca ccgcatcggc cacgaaatcg
      781 gcagccgcga cgaattgaag caggaagtcg gcgaaggcag cgtcacgagg gtccgcgaca
      841 gcgacaagcc cgaaggcgcc aatttgccgc gcttcaacca gggcaattcg aagctgaacc
      901 cgtaaggcgc ggccggcgac ccggagcggc tgtcgacccc aggagcagag tcgttcccgg
      961 gagcagagtc gttcccctgc gaaagcaggg gcccagccgg cgagatctct ggaccccggc
     1021 tttcgccggg gaacgggcgc tgtgtcgccg cggagccgct gccgctcccc ggaagcaggc
     1081 tcgttcccgg agcgaagtcg ttcccctgcg aaagcagggg tccagtcggc gagatctctg
     1141 gaccccggct ttcgccgggg aacggggctg ccgcgaatgg cagctccaat cattgccaga
     1201 gcgctttggc tcacatcccc gctcatgctg aggagggact gagcctgggc gaaggcccgt
     1261 ctcgaagcac gcacggtcct tcgagacgcc gcttcgccag gctcagcggc tcctcaggat
     1321 gagcgggtta ccctgcggaa caccagccac tcaggatgag cggcttaccc tccggaacac
     1381 caccgacaaa ttattagccg gcatctcgat aaccgagtcc agcctcaatc cctgccgctc
     1441 cgcctccgcc gcaaccgctt ccagatcccg caccccccat tccggattgc gatcccgcag
     1501 cgagcggtcg aacgcttcgt tgctgggcgc ggtcggcacg tcctgccggc gataggcgcc
     1561 gtagagatag agcggcgcgc ccggcgccag cagccgcgcc gcgccccgca tcagccccac
     1621 cgtcgccgcc cacgggctga tatggaccat gttgatgcac aggatcgcgg ccgcctcatt
     1681 caccggccac tcgccggcgg cggcgtcgag cgggaccggg gcgagcagat tgggcagccg
     1741 ctcggcggtg cgccaggcgt cgatcgagcg cagcccgacc gggtcggcgt cggtcggctg
     1801 ccacatcagg tgcggaaagg cggccgcgaa gtgaagcgca tgctcaccgc ttccgctcgc
     1861 cacctccagc accagccccg cgtcggggag gatgccgcgc aggacgacgg cgatcgggcc
     1921 ggcgttgcgg gcgacgtggg gcgccgagcg gcggtcgtcg ctcactcggc cgcctgcgcc
     1981 cggtccggct ccttatagac caaaaccggc ttcctcgccg cctgggtctc gtcgagccgc
     2041 cgccgcgggg cgtggaccgg ggcggccttc agcgactggt cgccggcccg tgcgcgttcg
     2101 gcgaccgagc gcagcgcgcc gataaactgg tcgagcgccg ccttgctctc ggtctcggtc
     2161 ggctcgacca gcatggcgcc gtggacgacc aacgggaaat acatggtcat cggatggtag
     2221 ccctcgtcga tcagcccctt ggcgagatcg agcgtggaaa ggccgccggc aaagcctttg
     2281 tcgctgaaca gcgcctcgtg catgcacggg ccgctggcgc cgaacggcgc gtccagcacg
     2341 tcctccaatt ggcgcagcac gtaattggcg ttgagcaccg catcgcccga gacctgcctc
     2401 aatccgtcgg tgccgtggct gaggatgtag gcgagcgcgc gggtgaacat gcccatctgg
     2461 ccgtggaagg cggtcatgcg gccgaaggtc gagccgtgat gctcgcccgc gctctcctcc
     2521 tcgatcagcg cataagcctc gccctgcttc tcgacgaacg gcaagggcgc gaacggggtc
     2581 agcgcctccg agaagaccac cgggccggag ccggggccgc cgccgccatg cggggtcgag
     2641 aaggtcttgt ggaggttgat gtgcatggcg tcgatgccga ggtcgcccgg gcggacgcgg
     2701 ccgacgatgg cgttgaaatt ggcaccgtcg caatagacca ggccgccggc ggcgtggacc
     2761 gcttcggaaa tggcggtcat gtcgggctcg aacaggccgc acgtattggg gttggtgatc
     2821 atcaccgcgg cgacgtcggg gccgagccgg ctcttcagcg cgtccagatc gacccggccg
     2881 cgatcgttgg cggggatgtt ctcgacccgg tagccggcga aggcggcggt ggcggggttg
     2941 gtgccgtggg cgctttcggg gaccaggacc acttggcggg cgtcgccgcg cgcctcgagc
     3001 gcggagcgga tcgcgagcag gccgcacaat tcgccatgcg cgcccgcctt ggggctcatc
     3061 gccaccgaat gcatgccggt gagcttgatc agccattcgc cgagatcggc gatcagcttc
     3121 agcgcgcctt ggaccgtgtc ggccggctgg agcgggtgga tgtcggcgaa gcccggcatc
     3181 cgcgcgacct tctcgttcaa tcgcggattg tgcttcatgg tgcacgatcc gagcgggaac
     3241 aggccgaggt cgatggcgta attctggcgg ctgagccgcg tataatgccg caccgtctgc
     3301 ggctccgaaa gcccgggcag cccgatcggg cgatgacgct ggaggccggc caacttatcc
     3361 tccccggaac ggggaggggg accgctcggc gaagccgagg ggtggagggg gcccgcgaag
     3421 tcgaaatcca ccccgcagcc gtcttcctcg ccgagctcga agatgagcgg ctcttccagc
     3481 atcagcgcgc ggttgccggt gaaggtgacg gcttcggcgc gctcggccgt tccgatttcg
     3541 ggcttccagc cgcttgcatt gatgctcatg ccagtatctc ctcgagcgcg ccggccagcg
     3601 cctcgacatc ttcgtcggtg acggtttcgg tgacggcgag gacgaggccg ttccggagcg
     3661 cctcgacgcc cggatagagc cggccgagcg acacgccgcc gagcatcccg cgctccgcca
     3721 gaatgcgcac caccggccgc gcctcgaccg gcagccgcaa ggtgaattcg ttgaagaagg
     3781 cctcattgac caattcgacc ccgggcaccc gcgccagccg ctcggccgcc gccaccgcct
     3841 tgacgtggtt gatctcggcc agccgcctca gcccggcctc gccgagcagg gtcatgtgga
     3901 cggtgaaggc gagcgcgcac aagccgctgt tggtgcagat gttggacgtc gccttttcgc
     3961 gccggatatg ctgctcgcgg gtcgacaaag tgagcacgaa gccgcgcttg ccctcggcat
     4021 cgacggtctc gccgcacagc cgaccgggca tctggcggac gaacttctcc ttgcaggcga
     4081 acaggccgac atagggcccg ccgaaattga ggccgacgcc gatcgcctgg ccttcgccga
     4141 cgacgatgtc ggcgcccatc tcgcccgggc tgcggatcgc gccgagcgcg accggctcgg
     4201 tcaccaccgc caccagcaac gcgccgttgg catgcgcctt ctcggcgatc ggcgtcaggt
     4261 ccgcgatgcg gccgagaatg tcgggatatt ggacgacgac gcagctcgtc tcgccgtcga
     4321 tcgcggcgag caggcgctcg gcgtcggtcg cggcggcaag ctccggcggc gccacgtcga
     4381 gccggtcgcc ggtgaaccgc gccatggtct cggccaccga cacataatgc ggatgcaggc
     4441 cggaggagag gatcgccttg ccgcgccgcg tcacccgccc cgccatggtg atcgcctccc
     4501 agcaggcggt cgagccgtca tacatggagg cgttggcgac gtcgcagccg tagagccgcg
     4561 cgacctgggt ctggaattcg aacaaggcct ggagcgtgcc ttgcgcgatt tccggctggt
     4621 agggcgtgta gctggtcagg aactcgccgc gctggatgat atggtcgacg ctggccggga
     4681 tatggtgcct gtaggcgccg gcgccgatga agaacggcac ctcgcccgcg accaggttgc
     4741 gccgcgcgag cgcgcccaat tgccgctcga ccgcaagctc gctggcatgg tcgggcaggc
     4801 cctcgatctt acccgccagc cgcgccgcct ccggcacgtc gacgaacagc tcctcgaccg
     4861 aggcggcccc gaccgcgcgc agcatctcct ggcggtcggc ttcagctaaa ggaaggtaac
     4921 gcatattgct gctccgtcat ccggacatgg ccgggctagt tctggtttgt tcaaggcagg
     4981 cggcaggtct cagtcgggct tgcgcatcga gaagcgcagg cggacataat cggcgatcca
     5041 gctgccgtcg ggctgctgga ggagcggctg gagccgcgcc tcgacggcgg cggccaccgc
     5101 ggcgcggtcg tccggcggca cttgcgcaac ctccagccaa ccg
//
LOCUS       DASVRK010000031         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_129739, whole genome shotgun sequence.
ACCESSION   DASVRK010000031 DASVRK010000000
VERSION     DASVRK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129739"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VF589_01055"
     CDS             <1..1352
                     /locus_tag="VF589_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEX8256192.1"
                     /translation="WEKDGHIFDAGPTVITDPDCLRELWALSGADMARDVDLVPVSPF
                     YRLSWPDGTVFDYLDDDERLAVQIAALDLADVDGYRRFLDYSAGVYREGYVKLGAVPF
                     LDFKAMLRAGPSLARYQAWRSVYSVVSGFVRNEKLRQALSFHTLLVGGNPMTTSAIYA
                     LIHKLERDGGVWFARGGTNQLIAGMVRHFERIGGSLRLGDPVAAIETEGGAARAVRTR
                     SGWSERFDAVASNADIVRTYEMLGHARGPKAAAKLRRKRFSPSLFVVHFSTRGEWPDV
                     PHHNILFGPEYHGLLREIYRGDGLPHQPSIYLHHPTATDPGMAPPGKSTFYALAPVPH
                     LGKAPLDWDKEGPVYRDRILRLLEERVLPGLGERIDHVFHYGPRDFEADLNSHLGSAF
                     SLEPILTQSAWFRVHNRDDAVGNLYFVGAGTHPGAGIPGVVGSAKATAGLMIGDLAA"
     gene            1349..2227
                     /locus_tag="VF589_01060"
     CDS             1349..2227
                     /locus_tag="VF589_01060"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX8256193.1"
                     /translation="MNRDELVAAAGASIRRGSKSFHMASRLFDRPTRERAWLLYCWCR
                     HCDDACDGQALGRPDPAGAQGSVEDAAALTRRALAGETTGILPFDALATLLRECEIPH
                     RLVLDHLRGFALDAAGWRPREETDLLAYCYHVAGAVGCMMAVVMGVDPEDEETLATAS
                     DLGIAFQLANIARDVREDHENGRCYLPERWMEQHGVDPRDPFRPDRRARLVTLVGRIT
                     DLAGRFEASAQAGIPKLPFRARWAVLAAARIYGAIGRRVAALGDTAWDGRVVVPRRAK
                     LAFLIPTLIASARTAR"
     gene            2235..>2975
                     /locus_tag="VF589_01065"
     CDS             2235..>2975
                     /locus_tag="VF589_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256194.1"
                     /translation="MAHFAFIAPPLRGHYKPLSHLAAELIARGHQATFIHHRDAADLV
                     EAEGAAFEAIGGGNAPPVSGWTRPMTRIRGVVGLGGMMSGMIRFTDAFCAEAPAVLRR
                     IGADALIVDQLEAGGGLVAEHLGLPFASVADTLPINREPGVPPPYVGWRYDPTARGEK
                     RNLGGWRVSDWLMRRLGEAIEANARRLGLAPRRRLEDCLSPRLQIAQLVPSLDFPRRE
                     LPSNFHYTGPFRRGAPAAFELPPGDSRPL"
BASE COUNT          393 a         1104 c         1015 g          463 t
ORIGIN      
        1 attgggagaa ggacggccac atattcgacg ccggcccgac cgtcatcacc gaccccgatt
       61 gcctgcgcga gctgtgggcg ctgagcggcg ccgacatggc ccgcgacgtc gatctcgtcc
      121 cggtctcgcc cttctaccgc ctgtcctggc ccgacgggac cgtattcgac tatctcgacg
      181 acgacgagcg gctggcggtg cagatcgccg cgctcgacct cgccgatgtc gacggatatc
      241 gccgtttcct cgactattcg gcgggcgtct accgcgaggg ctatgtgaag ctcggcgcgg
      301 tgccgttcct cgatttcaag gcgatgctgc gggcgggccc gtcgctcgcc cgctaccagg
      361 cctggcgctc ggtctattcg gtggtgtccg gcttcgtccg caacgagaaa ttgcggcagg
      421 cgctgtcctt ccacaccctg ctggtgggcg gcaatccgat gacgaccagc gccatctatg
      481 cgctgatcca caagctcgag cgcgacggcg gcgtatggtt cgcccgcggc ggcaccaacc
      541 agctgatcgc cggtatggtg cgccatttcg agcggatcgg cggcagcctc cggctcggcg
      601 atccggtggc ggcgatcgag accgagggcg gcgccgcccg cgccgtccgc acccgcagcg
      661 gctggagcga gcgcttcgac gcggtcgcct ccaacgccga catcgtgcgc acctacgaga
      721 tgctcggcca tgcgcgcggc cccaaggcgg cggccaagct gcgccgcaag cgcttttcgc
      781 cgtctttgtt cgtggtccat ttctcgaccc gcggcgaatg gccggacgtg ccgcaccaca
      841 atatcctgtt cgggcccgaa tatcacggct tgctgcggga aatctatcgc ggcgacggct
      901 tgccgcacca gccttccatc tacctccacc accccaccgc gaccgatccg gggatggcgc
      961 cgccgggcaa atcgaccttc tacgcgctgg cgccggtgcc gcatctcggc aaggcgccgc
     1021 tcgactggga caaggaaggc ccggtctatc gcgaccgcat cctgcgcctg ctcgaggaac
     1081 gggtcctgcc cgggctaggc gaacgcatcg accacgtctt ccactacggc ccgcgcgact
     1141 tcgaggccga tctcaattcc catctcggct ccgccttcag cctggagccg atcctgaccc
     1201 agagcgcctg gttccgggtc cacaatcgcg acgatgctgt cggcaacctc tatttcgtcg
     1261 gcgccggcac ccatccgggc gccggcattc cgggggtggt cggcagcgcc aaggcgaccg
     1321 ccgggctgat gatcggagat ctggcggcgt gaaccgggac gagctcgtcg ccgccgccgg
     1381 cgcctcgatc cggcgcggat cgaaatcctt ccacatggcc agccgcctgt tcgaccggcc
     1441 gacgcgcgag cgggcctggc tgctttattg ctggtgccgg cattgcgacg atgcctgcga
     1501 cggccaggcg ctcggccgcc ccgacccggc cggagcgcag gggagcgtcg aggacgccgc
     1561 cgcgctgacc cggcgcgcgc tggccggcga gacgaccggc atcctcccgt tcgacgcgct
     1621 cgccaccctg ctcagggagt gcgagatccc gcaccggctg gtgctcgacc atctgcgcgg
     1681 cttcgcgctc gacgcggccg gctggcggcc gcgcgaggag accgacctgc tcgcttattg
     1741 ctatcatgtc gccggcgcgg tcggctgcat gatggcggtg gtgatgggcg tcgacccgga
     1801 ggacgaggag acgctcgcca ccgcctccga tctcggcatc gccttccagc tcgccaacat
     1861 cgcccgcgac gtgcgcgagg accatgagaa tggccgctgc tacctccccg agcgctggat
     1921 ggagcagcat ggcgtcgacc cgcgcgaccc gttccgcccg gaccgccggg cgcggctggt
     1981 gacgctggtc ggccgcatca ccgacctcgc cggccgcttc gaagcctccg cccaggccgg
     2041 cattcccaaa ttgccgttcc gggcgcgctg ggcggtgctg gcggcggcgc gcatctacgg
     2101 cgcgatcggg cggcgggtcg ccgcgctcgg cgacaccgcc tgggacgggc gggtggtcgt
     2161 gccgcgccgc gccaagctcg cctttctgat ccccaccctg atcgcttcgg cgcgcaccgc
     2221 ccggtaagcc ggccatggcc catttcgcct ttatcgcgcc gccgctgcgc ggccattaca
     2281 agccgctgtc gcacctcgcc gccgagctga tcgcgcgcgg ccaccaggcg accttcatcc
     2341 accatcgcga cgccgccgac ctggtcgagg ccgagggcgc cgccttcgaa gcgatcggcg
     2401 gcgggaacgc gccgccggtg agcggatgga cgcggccgat gacccggatc cgcggcgtgg
     2461 tcggcctcgg cggcatgatg agcggcatga tccgcttcac cgacgccttc tgcgccgaag
     2521 ccccggcggt gctgcgccgg atcggcgccg acgcattgat cgtcgaccag ctcgaggcgg
     2581 gcggcggcct cgtcgccgag catctcggcc tgccgttcgc gagcgtcgcc gacacgctgc
     2641 cgatcaaccg cgagccgggg gtgccgccgc cttatgtcgg ctggcgctac gatccgaccg
     2701 cgcgcggcga gaaacgcaac ctcggcggct ggcgggtctc cgactggctg atgcgccgcc
     2761 ttggcgaggc gatcgaggcc aatgcccggc ggctcgggct ggcgccgcgg cggcggctgg
     2821 aggattgcct gtcgccgcgc ctgcagatcg cgcagctggt gccgagcctc gactttccgc
     2881 ggcgggagct tccgtccaat ttccattata ccggcccgtt ccgccgcggc gcgccggcgg
     2941 cgttcgagct gccgccgggc gacagtcgcc cgctg
//
LOCUS       DASVRK010000032        20284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_564744, whole genome shotgun sequence.
ACCESSION   DASVRK010000032 DASVRK010000000
VERSION     DASVRK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564744"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..723)
                     /locus_tag="VF589_01070"
     CDS             complement(124..723)
                     /locus_tag="VF589_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX8256195.1"
                     /translation="MAQPVRPLEPVIATSRRPFADSRQRGPTVRPPQPDDLNSALERN
                     IEALQQRRKAEAEKASREAKIAEAITRFTGSMLFVWLHLAVFGFWILANLGWVPGVPS
                     WDPSFVVLAMIASVEAIFLSTFVLISQNRMAAAADKRADLDLQINLLAEHEVTKLVAM
                     VSEISRHLGLDHAVEEDVEELKRNVAPEAVLDAIDRRQE"
     gene            complement(754..2028)
                     /locus_tag="VF589_01075"
     CDS             complement(754..2028)
                     /locus_tag="VF589_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX8256196.1"
                     /translation="MSQPTRILLALVVGLLLGIASAATGGGWVEGAVSVAEPVGGMWL
                     NALRMTIVPLVVSLLVTGIAASAEAARAGKLATRALVLFVVLLWISAIAAALLTPLFL
                     GLFPLPAESTAALRAAMTASEPVGEVPGFADFLSSIVPTNPVAAAAEDAILPLILFTL
                     VFAFALTRLPAEPRERLVGFFQAIADTMLVVIGWVLWIAPIGVAALAYVVGARAGGAA
                     FGALLHYVLVVTAVGTVIWLAAWPLAMFGAKVPLGRFTRAIAPSQAVAFSTQSSLASL
                     PAMLRGAGAIGVPVAASGVVLPMAVAVFRATGPAMNLAVAIYIAYWFGIELTPAQMAI
                     GVAAAATTTMGAVGIPGQASFITSIAPICLAMGVPIEPLALLIAVEVLPDIMRTLGNV
                     SMDVAATATIAKRSGFEAGEATTEEDRLLGEA"
     gene            2114..3448
                     /gene="glmM"
                     /locus_tag="VF589_01080"
     CDS             2114..3448
                     /gene="glmM"
                     /locus_tag="VF589_01080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX8256197.1"
                     /translation="MARKFFGTDGIRGRTNEMPMTAAMAQRVGQAAGAHFLRGSHRHR
                     VVLGKDTRLSGYMMETALISGFTSVGMDVVMVGPVPTPAVAMLAKSMRADLGVMISAS
                     HNPFEDNGIKLFGPDGYKLSDENELAIEALMEREPKLVAPDKIGRARRIEDARGRYIH
                     AAKTTFPEHLRLDGLRVVVDCANGAAYQVAPSAFWELGADVTAIGVSPNGLNINDGCG
                     STKPELLQRTVIEAGAHIGIALDGDADRLIVVDEQGEVVDGDQLMALIASSWQRRGML
                     RGRGIVATIMSNLGLERFLAGKGLALERTQVGDRYVLEAMRAKGFNIGGEQSGHIILS
                     DYATTGDGLVAALQVLSELVVQGRPASELLRQFEPLPQLLKNVRFDGGTPLETDGVRS
                     AIASAELRLQGSGRLVIRKSGTEPLIRVMAEGEDRALVQEVVDSICAAVKAA"
     gene            complement(3641..5416)
                     /locus_tag="VF589_01085"
     CDS             complement(3641..5416)
                     /locus_tag="VF589_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8256198.1"
                     /translation="MADISAEAPPSRKLGNLAMIWRFAIRYPAQIGAAALALFVAAAA
                     TLAIPDGFRRVIDKGFAAAGGAIAEHFYYLLGIVIVLSMATAVRFYFVSWLGERVVAD
                     VRTAVHRNLLRLEPRFFEENRPSEIASRLTADTSVIEMVVGGTVSVALRNIVMGLGGI
                     IYLFTLAPRLTGTLLLGIPIVIVPVVLLGRKIRDVSRYSQDRIADVGAIVSETLSAMK
                     IVQAFGQEDRESERFDGAVQEAFGIAKRRIVLRALMTAVIIALMFGSITLVMWQAAID
                     VAEGRLTGGTVAAFVFTGALVAGAFGALTEVYGELLRGAGAAGRLAELLAQVPEIQSP
                     ADPVRLPQPARGALSFDDVTFHYPTRPEVSALKRFTLHVAPGEMLAVVGPSGAGKTTL
                     FQLAQRFYDPEGGAVRLDGVDLRAADLADIRARIAVVPQETVIFAASARDNLRYGRWD
                     ASDDELWAAAEAANAADFLRALPQRLDTFMGEGGARLSGGQRQRVAIARALLRDAPLL
                     LLDEATSALDAESERAVQASLERLMESRTTIVIAHRLATVRAADRIVVMDSGEIVEEG
                     THDSLNARGGLYARLARLQFEGLAA"
     gene            5562..6782
                     /gene="phaZ"
                     /locus_tag="VF589_01090"
     CDS             5562..6782
                     /gene="phaZ"
                     /locus_tag="VF589_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX8256199.1"
                     /translation="MLYDAYEMQRSLLAGASTWANIGAGWMQNPANPFAYSSMGPIVA
                     SALDVFAHASASRGKPDFGLDTTIVEGKSVAVTEEIVLRKPFGQLKRFRRAGVEGGPR
                     LLIVAPMSGHYATLLRGTVERMLPGHDVYITDWRDAKLVPVDSGRFDLDDYIEYLVEY
                     LAHIGPGAHMLAVCQPSVPCYAAAAVMSRDKHPARPRTLTMMGGPIDTREAPTAVNTL
                     ATQRPHSWFQRNVIATVPYPYPGAGRHVYPGFLQLAGFMSMNLGSHLTSHWEMFKHLV
                     QGDGESADASKEFYDEYRAVCDMTEEFYLQTVDVVFQRHLLPKSEMMHRGRRVDPAAI
                     DDVAILAIEGERDDISGIGQTKAALDISTGLAPALKHYHLAKGVGHYGIFNGSKWRDR
                     IAPVVENWISRHDG"
     gene            complement(6837..8390)
                     /locus_tag="VF589_01095"
     CDS             complement(6837..8390)
                     /locus_tag="VF589_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256200.1"
                     /translation="MAVIKGDEGDNVLIGTRESDSLSGFGGSDKLYGLRGNDELDGGE
                     GADEMFGRFGDDTYWVDDENDRVAEFRGEGFADMVISSIDYRLPVGVENLILIASAIA
                     GRGNSLDNRMVGNDGDNRLDGGEGADTMEGGRGDDTYVLRGSPEDRVIEFEGEGTDAV
                     ILYLASATQGYTLAANLENVRQTGDAGTIFGNDAANYIIGSSFADDLRGGVGDDVLVG
                     LDGFDSLVGGAGADLMRGGTGGDTYTVDNAGDRVLEWSDEGNDVVLSSVSYTLSGNIE
                     RLDLSAGGIRGVGNRLANRIVGNSERNILDGRGGDDVMEGGFGNDDYYVSSTKDVVID
                     TFNGGHDRIFSSSSFQIAGSVEDLFLIGTVSTNGYGNSNINRIVGNEAANSLYGGDGW
                     DQLAGGDGDDKLYGGDGRDTLEGGAGADGFYFDDQPDGDRILDFAPADDTIFLSRARF
                     NTLVVGTLDVDAFHEGTVAQDAEDRIIYNRATGEIYFDGDGNGRGTPLLLATVTPETD
                     LTHADFVVY"
     gene            complement(8460..9788)
                     /locus_tag="VF589_01100"
     CDS             complement(8460..9788)
                     /locus_tag="VF589_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256201.1"
                     /translation="MREFRNEGLADVVFSSVSFKLQKNIENLVLIGTDSLVGRGNDYE
                     NHIVGNSGDNRLDGGLGADILEGGLGNDVYYLTENGETRIERADEGEDTVISSLSHSL
                     AEHFEILRLTGAAVSGNGNAAANRLYGNEAANVLNGAAGADLMYGGGGDDTYHADHLG
                     DRVTERRGEGRDTVISSASIRLYGEIEVLSLTGNATHAWGSATANQIFAQTAVIDNVL
                     DGGGGADFMSGSYGADTYIVDHRNDTIDEKNTFDNKIDLVRASVSYTLAFDYSGFVED
                     MVLTGSAAINGTGNIAGNRIVGNDADNVLKGDLGSDTLIGGGGDDSLYGQRSSDDKLV
                     GGAGNDGFYFEDVQYGFYIADIIKDFSSVDDTIFLDRDAFKQIGEGTLDPNAFVVGTQ
                     AQDADDRIIYDPDTGQVKYDPDGFGGSFGMFLAILTPGTVIDHTDFVGFD"
     gene            complement(9737..9985)
                     /locus_tag="VF589_01105"
     CDS             complement(9737..9985)
                     /locus_tag="VF589_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256202.1"
                     /translation="MAVIKGDSGDNVLIGTRESDSLSGFGGDDRLYGLRGNDDLDGGE
                     GADLMVGRFGDDTDGLTISAMWCASSATRDWPTSYFLR"
     gene            10111..11202
                     /locus_tag="VF589_01110"
     CDS             10111..11202
                     /locus_tag="VF589_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8256203.1"
                     /translation="MRLLASWAALAVLSAAAPAAAQPDHAARLKEGEVVLRDFRFASG
                     ETLPELRIHYATLGSPRRDASGRIANAVMVLHGTGGSGRQFLQPQFAGELFGPGQPLD
                     LRRYYVILPDNIGHGKSSKPSDGLRMAFPKYDYDDMVEAQRRMLAEGLKVGSLRLLMG
                     TSMGCMHGFVWAARHPGFARAMMPMACLPVEIAGHNRMWRKAAVEGITSDPAWAGGNY
                     RTQPVQGLRTAVSLLQVAGFAPLYLQKAYPTRAAADAYIVERIDKDLPSRDANDLIYQ
                     LEASRTYNPWPTLERINVPMTWINSSDDFINPAEYGIAEDAARRMPSVRYILVRATSD
                     TRGHGTHTWAKFWRQELSDLLARTERDDP"
     gene            11237..12316
                     /locus_tag="VF589_01115"
     CDS             11237..12316
                     /locus_tag="VF589_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256204.1"
                     /translation="MKLLASLAAMPLLLLAPIGADAAAPKEGRFPPLGDCRDDRGVDR
                     CDPAQHRRVLALYGVQPIEAHLAAGDIVRRAFFVDGYGSDRVAISFVRAKGRDPALWV
                     HVPSGKEQGRAPMQAPVPGPVWDDLIRRSAHFDRTLAPPKPGPDGEEEVLCIHSWVYT
                     LEATELPDELAGQPGRVRRKTEDACDDGLAEAYALELARAAVPLLPACALIDPRQQRG
                     LPGVLSACFLLTGDRLAAAEVLNRLNLIQAAENESDAARAEQVFGPEAQLDWQGSRSA
                     DRAQAAKLWLAKLAEGGGAQLFYDKVEGLSPDRVRFVGFLSRYAGEANGESVNQFASV
                     EQIWTREGGDFFIERAIVGPWERER"
     gene            complement(12313..12792)
                     /locus_tag="VF589_01120"
     CDS             complement(12313..12792)
                     /locus_tag="VF589_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX8256205.1"
                     /translation="MKTTSRAALSLALLATLCACSAPSAEAAAATPETEKAAIRQVIA
                     DMEAAWNRGDFRGYMAGFKRPDVVFVSRGEFQKDWQGTLDHYVRDYGGSPERRGKLRF
                     SDIRIELLAPDAAQLISRYRLEGGGSPQDGINTRLMRKVDGRWVIALNHVSSKEVRR"
     gene            12988..13374
                     /locus_tag="VF589_01125"
     CDS             12988..13374
                     /locus_tag="VF589_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256206.1"
                     /translation="MNKLIGKSIIGAAAAVTLAATAAPAEARHWRHGGDGDALAAGII
                     GLGIGAAIASSHHGGYYGGGHYGGGYGGGYYGRPAYYGAPVYYGRPAYYGRPQYRPHH
                     GYYGRRCFTRRVWDPYWGRRVRVRVC"
     gene            complement(13405..15462)
                     /locus_tag="VF589_01130"
     CDS             complement(13405..15462)
                     /locus_tag="VF589_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8256207.1"
                     /translation="MSPITTRRHGDVLIVTSNNPPVNALSHSVREGLVKAIDQAEADE
                     AVQAVVILCEGNTFFAGADITEFGKPMQHPWLPQVVDRIEASAKPVVAAIHGTALGGG
                     LEVALACHYRVAVPNATMGVPEVKLGLLPGAGGTQRLPRVAGVTAALEMAATGNPVRA
                     QKAYEIGLLDKLIEGDLEQHAVAYAHEVKDVRPLPKSSERDDKLAEARSNPDIFREFR
                     KANARKFRGFEAPEANIRAIEAAVAKPYEQGVMYERQLFMELMSGNQARAQQYFFFAE
                     RQAAKIEGLPEGTRPRDIRRVGVIGAGTMGGGISMNFLSAGIPVTIVEMAQDALDRGA
                     GVMRKNYEATAAKGRMTSDQVEQAMGLLNPTLDFDALAECDLIIEAVFENMDVKKEVF
                     GRLDKIAKPGAILASNTSYLNIDEIAASTSRPQDVLGLHFFSPANIMKLLEVVRGAKT
                     APDVLMTAMQLARKIRKVAVVAGVCYGFIGNRMLIPRQTQAMRLLLEGATPEQVDRVH
                     TDFGMPMGPFQMADLAGVDIGWHRDPNRIENIRDALCAIDRWGQKKGAGFYDYDEKRR
                     PSPSPKVQEIIEGFRAREGVEAREIDDQEIVERTLYTMVNEGAKILEEGMAQRASDVD
                     VVWVYGYGWPVYRGGPMFWADSEGLAKIVEGITRQQQRLGADFTLSELLTRKAAAGEK
                     LTR"
     gene            complement(15475..16266)
                     /locus_tag="VF589_01135"
     CDS             complement(15475..16266)
                     /locus_tag="VF589_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8256208.1"
                     /translation="MGRLQGKVAIVTGAGSGIGRAAAALFAGEGAFVVVSDISEAVEE
                     TAALIGPAAVAVRGDAGSDEDVAHLVRSAAARFGGLDIVFANAGVSGGLASILEQGPE
                     DWAEILRVNLIGPALAIRHAAPLIRERGGGSIICTASVAGLRSGAGGPAYSASKAGVI
                     SLVKTSAQQLSGSNVRVNAICPGLIETGMTEFVYERARAKGQEDRLGHLNPLKRGGSP
                     GEIAAAALFLASEESSYVNGHALVVDGGLASSLPFNTQSYGRTAI"
     gene            complement(16379..17155)
                     /locus_tag="VF589_01140"
     CDS             complement(16379..17155)
                     /locus_tag="VF589_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8256209.1"
                     /translation="MNLFDLSGKVAVITGSSRGIGRAIAEAMAEHGAKVVISSRKAEA
                     CEEAAQAINECHGDGTAIVVPANISSKQDLQNLVDETRRRLGRIDVLVCNAASNPYYG
                     PMAGIADEQFRKILENNVIANHWLIAMAAPEMIERKEGSIIIVSSIGGMTGSTMIGAY
                     NISKAADFQLARNLAAEFGPAGVRVNCIAPGLVRTDFARALWENPDTLKAVTRGTPLG
                     RIGEPHEIAGAAVFLASPASTFMTGQTIVVDGGSTIGVGL"
     gene            complement(17157..18491)
                     /locus_tag="VF589_01145"
     CDS             complement(17157..18491)
                     /locus_tag="VF589_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8256210.1"
                     /translation="MDFDLTERQAFYRDRVRDFVETRIRPRTGDYKAQLAAGDRWQPV
                     EVIEELKPAAKAAGLWNLFMPPGHALAHVDESFEFEGTQLTNLEYALCAEEMGRILWA
                     SEVFNCSAPDTGNMEVLHRYGTREQKDEWLRPLMNGEIRSAFLMTEPAVASSDATNIE
                     TEIRRDGEDYVLNGRKWWSSGAGDPRCKVAIVMGKTDTGAHRHRQQSMVLVPLDAPGV
                     TVERALTVYGYDDAPHGHMEIELKDVRIPAANMLLGEGRGFEIAQGRLGPGRIHHCMR
                     TIGAAEEALAAMVRRLQTRVAFGKKLMEHSIWEQRVARARIDIDMSRLLCLKAADLMD
                     KAGNKAAQAEIAMIKVQAPNMALRIIDDAVQAHGGAGVSQDFGLAYSWAGIRTLRLAD
                     GPDEVHNRAIARLEFAKHASHGGGAGAEGAAARRPLHQPLAGPPPLEKEGRN"
     gene            18709..19881
                     /locus_tag="VF589_01150"
     CDS             18709..19881
                     /locus_tag="VF589_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8256211.1"
                     /translation="MNPSVDHDTFRFETRGWLEANCPPEMRQPMRDEGDACWGGRNPV
                     FQSEAQKNWLDRMAARGWTVPDWPSDYGGGGLSPAQTKILRQEMTAIGARNPLYSFGI
                     SMLGPALLKYGSEEQKRRFLPEIARGEIRWCQGYSEPNAGSDLASLQTRCEDKGDHWL
                     VNGQKVWTSYADKADWIFCLVRTDFEAPKHKGISFLLFDMASPGVSTRPILLISGSSP
                     FCETFFDDVRVPKDQVVGEVHKGWDVAKYLLGHEREMISGMGLGGQGGSLGAALGERL
                     ADDPILRADVARFDVDAMAFRAMSERFLDELKAGQAHPAQPSMMKYAGTELNKARHEL
                     IMAAGGSNALEWETERSNGGKAAREWLRTKANSIEGGTSEIQLGIIAKHILQLPGA"
     gene            19885..>20284
                     /locus_tag="VF589_01155"
     CDS             19885..>20284
                     /locus_tag="VF589_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8256212.1"
                     /translation="MPLYLTEDQVMLRDTARDFMASEGAIATQLRKFRGMNCKDGFGH
                     ALWRQFGEMGFTGILVDEADGGLGLGHVEAGIVLEEIGRNLTPSPFLTTAVAVVTALR
                     GAGEELRARWLPGILAGETIAALAVDEGPKH"
BASE COUNT         3288 a         6817 c         6799 g         3380 t
ORIGIN      
        1 atggcgacgg gcccggtgag gagggcgccc cggattatcg ggtcaactga gcgcgcttgc
       61 cgaggggagc acccagccgg attgacgccg ctccctcgga acccggacga tagccgacgc
      121 ccgtcactcc tgccgccggt cgatggcgtc gagcaccgct tcgggggcga cgttgcgctt
      181 cagctcctcg acatcctcct cgaccgcatg gtcgagcccg agatggcggg agatttcgga
      241 caccatggcg accagcttgg tcacttcatg ctcggcgagc aggttgatct ggaggtcgag
      301 atcggcgcgc ttgtcggccg ccgccgccat ccggttctgg ctgatgagga cgaaggtcga
      361 caggaagatc gcctcgaccg acgcgatcat ggcgaggacg acgaaggacg ggtcccagga
      421 cggcaccccc ggcacccagc cgagattggc gaggatccag aagccgaaca cagccagatg
      481 cagccagacg aagagcatcg atccggtgaa gcgggtgatc gcctcggcga tcttggcttc
      541 gcgcgaagcc ttttcggcct ccgccttccg ccgctgctgc agcgcctcga tattgcgctc
      601 gagcgcgctg ttgaggtcgt ccggctgggg cggccggacg gttggcccgc gctggcggct
      661 atcggcaaac gggcgtcgag aggtggcgat gacgggctcc agcggtcgaa caggctgcgc
      721 caacaaccgg ctaggcgcca agctgttcct gcttcaggct tcgccgagca gccgatcctc
      781 ttcggtggtc gcctcgcccg cctcgaagcc gctgcgcttg gcgatggtgg cggtggcggc
      841 gacgtccatg ctgacattgc cgagcgtgcg catgatgtcg ggcaggacct cgaccgcgat
      901 cagcagcgcc agcggctcga tcggcacgcc catggcaagg cagatcggcg cgatcgaggt
      961 gatgaagctc gcctggccgg ggatgccgac cgcgcccatg gtggtggtgg cggccgcggc
     1021 gacgccgatc gccatctgcg ccggcgtgag ctcgatgccg aaccagtaag cgatgtagat
     1081 cgccaccgcg aggttcatcg ccgggccggt ggcgcggaac accgccaccg ccatcggcag
     1141 caccacgccc gatgcggcga ccgggacccc gatcgcgccg gcgccgcgca gcatcgccgg
     1201 cagggaggcg agcgaggatt gggtgctgaa ggcgaccgcc tggctcggcg cgatcgcgcg
     1261 ggtgaagcgg ccgagcggca ccttggcgcc gaacatcgcc aatggccagg cggccagcca
     1321 gatcaccgtg ccgaccgcgg tcaccaccag cacataatgg aggagggcgc cgaaggccgc
     1381 gccgccggcc cgggcgccga ccacgtaggc gagcgcggcg accccgatcg gcgcgatcca
     1441 cagcacccag ccgatcacca ccagcatggt gtcggcgatc gcctggaaga agccgaccag
     1501 ccgctcccgc ggctcggccg gaagccgggt cagcgcgaag gcgaagacga gggtgaacag
     1561 gatgagcggc aggatcgcat cctcggccgc cgccgcgacg ggattggtgg ggacgatcga
     1621 gctcaggaag tcggcgaagc cgggcacttc gccgacaggc tcgctcgcgg tcatcgccgc
     1681 gcgcagcgcc gcggtcgatt ccgccggcag cgggaacagg ccgaggaaca aaggcgtgag
     1741 caaggcggcg gcgatggccg agatccacaa caggacgacg aatagcacca gcgcccttgt
     1801 ggcgagcttg ccggcccgcg cggcctcggc cgaggcggcg atcccggtca ccagcagcga
     1861 gacgaccaaa ggcacgatgg tcatgcgcag cgcgttcagc cacatgccgc cgaccggctc
     1921 ggcgacggag accgcgccct cgacccagcc gccgccggtg gcggcgctgg cgatgccgag
     1981 cagcagcccg acgacgaggg cgagaaggat acgcgtgggc tgcgacatca aaagcggttc
     2041 ccaactttcc ggccaatgct ctacagcctc ttcatggcgg gcccgccggg ggcaagcgaa
     2101 aacggacggg cagatggcgc gcaaattctt cggcaccgac ggcatcaggg gccggaccaa
     2161 cgaaatgccg atgaccgcgg cgatggccca gcgggtcggc caggcggcgg gcgcgcattt
     2221 cctgcgcggc agccaccgcc accgcgtcgt gctcggcaag gacacccggc tctccggcta
     2281 catgatggaa accgcgctca tttcgggctt caccagcgtc ggcatggacg tggtgatggt
     2341 cgggccggtg ccgacgccgg cggtggcgat gctggccaaa tcgatgcgcg ccgacctcgg
     2401 cgtgatgatc tcggccagtc acaatccgtt cgaggataac ggcatcaaat tgttcggccc
     2461 cgacggctat aaattgtcgg acgagaacga gctcgccatc gaagcgctga tggagcgcga
     2521 acccaagctg gtcgctcccg acaagatcgg ccgcgcccgc cgcatcgagg atgcgcgcgg
     2581 ccgctacatc catgccgcca agaccacctt ccccgagcat ctccggctcg acggcctgcg
     2641 cgtcgtcgtc gattgcgcca acggcgccgc ctaccaggtc gcgccctcgg ccttttggga
     2701 gctgggcgcc gacgtcaccg cgatcggcgt ttcgcccaac ggcctcaaca tcaatgacgg
     2761 ctgcggctcg accaagccgg aattgctcca acgcacggtg atcgaggccg gcgcgcatat
     2821 cggcatcgcg ctcgacggcg acgccgaccg gctgatcgtg gtcgacgagc agggcgaggt
     2881 ggtcgacggc gaccagctga tggcgctcat cgcctcctcc tggcagcggc gcggcatgct
     2941 ccgcggccgc ggcatcgtcg ccaccatcat gtccaatctc ggcctcgaac gcttccttgc
     3001 gggcaagggc ctggcgctcg agcggaccca ggtcggcgac cgctatgtgc tggaggcgat
     3061 gcgcgccaag ggcttcaata tcggcggcga gcaatccggc cacatcatcc tcagcgatta
     3121 tgcgaccacc ggcgacgggc tggtggccgc gttgcaggtc ctgtcggagc tggtcgtgca
     3181 ggggcggccg gcgagcgagc tgctccgcca gttcgagccg ctgccgcagc tcctcaagaa
     3241 cgtccgcttc gacggcggaa cgccgctcga gacggacggg gtcagatcgg cgatcgcctc
     3301 ggccgagctc cgcctccagg gcagcggccg gctggtgatc cgcaaatcgg gcaccgagcc
     3361 gctgatccgg gtgatggccg agggcgagga ccgggcgctg gtccaggaag tggtcgacag
     3421 catctgcgcg gcggtgaagg cggcctaggg aggcgggggc ggagagggcg gttgcggcgc
     3481 tgaaagtccc ctcgtcatgc ctggcgcttg ctcgtcatgc cggacttgat ccggcatccc
     3541 gcttcttctt tccagctcag gacgtgaaga agagagaccc cggatcaagt ccggggtgac
     3601 gtttcgatgg gatggtcgaa aattcacgcg aacctgacgc ctaggccgcc agcccttcga
     3661 actggagccg ggcgaggcgg gcgtagaggc cgccgcgggc gttgaggctg tcgtgggtgc
     3721 cttcctcgac gatctcgccg ctgtccatga cgacgatgcg gtcggcggcg cggacagtgg
     3781 cgaggcggtg ggcgatgacg atggtggtgc ggctttccat cagccgctcg agcgaagcct
     3841 ggacggcgcg ctcggattcg gcgtcgagcg cggacgtcgc ctcgtcgagc agcagcaggg
     3901 gcgcgtcgcg gagcagggcg cgggcgatgg cgacgcgctg gcgctggccg ccggacaggc
     3961 gcgcgccgcc ctcgcccatg aaggtgtcga ggcgctgcgg caaagcgcgc aggaagtcgg
     4021 ccgcattggc cgcctcggcc gccgcccaca attcgtcgtc gctcgcgtcc cagcgcccgt
     4081 agcgcagatt gtcgcgcgcc gaggcggcga agatcaccgt ctcctgcggg acgaccgcga
     4141 tccgcgcgcg tatgtcggcg aggtcggcgg cgcgcaggtc gacgccgtcg agcctcaccg
     4201 cgccgccctc gggatcgtag aagcgctggg cgagctggaa caaggtggtc ttgccggccc
     4261 ccgacggccc caccaccgcc agcatctcgc cgggcgcgac gtggagcgtg aagcgcttga
     4321 gcgcgctcac ctcgggccgg gtcgggtagt ggaaggtgac gtcgtcgaag ctcagcgcgc
     4381 cgcgcgccgg ctgcggcagc cggaccgggt cggcggggga ttggatctcg ggcacctgcg
     4441 ccagcaattc ggccagccgg ccggcggcgc cagcgccgcg cagcagctcg ccatagactt
     4501 cggtgagcgc gccgaacgcg cccgccacca gcgcgccggt gaagacgaag gcggccaccg
     4561 tgccgccggt cagccggccc tcggcgacgt cgatcgccgc ctgccacatg acgagcgtga
     4621 tcgatccgaa catcaaggcg atgatcaccg ccgtcatcag cgcgcgcagc acgatccgcc
     4681 gcttggcgat cccgaacgcc tcctgaaccg cgccgtcgaa gcgctcgctc tcgcgatcct
     4741 cctgcccgaa cgcctgcacg atcttcatcg cgctcagcgt ctcggacacg atcgcgccga
     4801 catcggcgat ccggtcctgg ctgtagcgcg agacgtcgcg gatcttccgc ccgagcagca
     4861 ccaccggcac gatcacgatc gggatgccga gcagcaaagt cccggtcagc cgcggcgcga
     4921 gcgtgaacag atagatgatg ccgccgagcc ccatcacgat gttgcgaagc gccaccgaaa
     4981 ccgttccgcc caccaccatc tcgatcacgg aggtgtcggc ggtcagccgc gaggcgattt
     5041 cggacgggcg attctcctcg aagaagcgcg gctcgagccg gagcaggttg cggtggacgg
     5101 cggtgcgcac gtcggcgacg acccgctcgc cgagccacga gacgaagtag aagcgcaccg
     5161 ccgtcgccat cgacagcacg atcacgatgc cgagcaaata gtagaaatgt tcggcgatcg
     5221 cgccgcccgc cgcggcgaag cccttgtcga tgacgcgccg gaaaccatcc gggatggcga
     5281 gggtcgcggc ggcggcgacg aacagcgcca gcgcggccgc cccgatctgc gccggatagc
     5341 ggatcgcgaa gcgccagatc atggcgaggt tgccgagctt cctgctgggc ggggcttcgg
     5401 cgctgatgtc tgccatggcg gcggactagc agcggcgggg gcgcggctca atggcaacac
     5461 tccggaaggg cgtcacggac ccttcgaccg accggtcggt ttatacggtg gattattcgc
     5521 cgtcgcgcgc gctatgatgc ggccgacggg tgggagagtg aatgttatac gacgcttatg
     5581 agatgcagcg ctcgctgctc gcgggagcca gcacctgggc caatatcggc gccggctgga
     5641 tgcagaaccc ggccaacccc ttcgcttata gcagcatggg accgatcgtc gcttcggcgc
     5701 tggacgtgtt cgcgcatgcc tcggcctcgc gcggcaagcc ggacttcggg ctcgacacga
     5761 caatcgtcga aggcaagtcg gtcgcggtca ccgaggagat cgtgctgcgc aaaccgttcg
     5821 gccagctgaa gcggttccgc cgcgccggcg tcgaaggcgg cccgcggctg ctgatcgtgg
     5881 cgccgatgtc gggccattat gcgacgctgc tgcgcggcac cgtcgagcgg atgctgcccg
     5941 gccacgacgt ctacatcacc gactggcgcg acgcgaagct ggtgccggtc gattccggcc
     6001 gcttcgacct cgacgattat atcgagtatc tggtcgagta tctggcccat atcggccccg
     6061 gcgcgcacat gctcgccgtc tgccagccgt cggtgccctg ctatgccgcc gccgccgtga
     6121 tgtcgcggga caagcatccg gcgcggccgc ggacgctgac catgatgggc ggcccgatcg
     6181 acacgcgcga agcgccgacc gcggtcaaca cgctggcgac gcagcggccg catagctggt
     6241 tccagcgcaa cgtcatcgcc accgtcccct atccctatcc gggcgcgggg cggcacgtct
     6301 atccgggctt cctgcagctt gccgggttca tgagcatgaa cctcggcagc cacctcacca
     6361 gccattggga gatgttcaag catctcgtcc agggcgacgg cgagagcgcg gacgccagca
     6421 aggaatttta cgacgaatat cgcgccgtct gcgacatgac cgaggaattc tacctgcaga
     6481 cggtcgacgt cgttttccag cgccatctgc tccccaaaag cgagatgatg caccgcggcc
     6541 gccgcgtcga tccggccgcg atcgacgatg tcgccatcct cgccatcgag ggcgagcgcg
     6601 acgatatctc cggcattggc cagaccaagg cggcgctcga catctcgacc ggcctcgcgc
     6661 ccgcgctcaa acattatcat ctcgccaaag gcgtcggcca ttacggcatc ttcaacggca
     6721 gcaaatggcg cgaccggatc gccccggtgg tggagaattg gatttcccgc cacgacggtt
     6781 gaaggccagg cgcgagcctc cgtccgacgg agcggcgcgc cgagcctgag cttgtcctaa
     6841 tagacgacga agtccgcgtg ggtgagatcg gtctcgggcg taacggtcgc aagcagtaac
     6901 ggcgtgcccc taccgttccc gtcaccgtcg aaatagattt cgcccgtggc gcggttgtag
     6961 ataatgcggt cttccgcgtc ctgtgccacc gttccctcat ggaaagcgtc cacatcgagg
     7021 gtgccgacca cgagtgtgtt gaaccgggca cgggacagga agatcgtgtc gtcggccgga
     7081 gcgaagtcga ggatgcgatc gccgtccggc tgatcgtcga aatagaagcc gtccgcgccc
     7141 gccccgccct cgagggtatc cctgccatcg ccgccgtaga gtttgtcgtc gccgtcgccc
     7201 cccgccaatt ggtcccagcc gtcgccgccg tagagagagt tcgccgcctc gttgccgaca
     7261 atccggttga tgttgctgtt gccgtagcca ttggtggaga cggtgccgat caggaagagg
     7321 tcctcgaccg agcccgcgat ttggaaggag ctcgagctga agatccggtc atggcctccg
     7381 ttaaaggtgt caatgactac atccttggta ctgctcacgt agtaatcgtc gttgccgaat
     7441 ccgccttcca tcacatcgtc gccgccgcgc ccatcgagga tgttcctctc ggaattcccg
     7501 acgatccgat tggcaagccg attcccgacc ccgcgtatcc cgcctgcact cagatcgagc
     7561 cgttcaatgt tgccgctgag cgtgtagctg acggaactga ggaccacgtc attgccttcg
     7621 tcgctccatt cgaggactcg atcgcctgca ttgtcgacgg tgtaggtatc cccccccgta
     7681 ccccctcgca tgaggtcggc gccggccccg cccactagcg agtcgaagcc gtccagtccg
     7741 acgagcacgt cgtcgccgac gccgcctcgc aaatcatccg cgaaagacga gccgataata
     7801 taattggcgg cgtcattgcc gaagatggtg ccggcgtctc cagtctgccg cacattttcg
     7861 aggttcgccg cgagggtgta gccctgtgta gcactggcca ggtaaaggat gacggcgtct
     7921 gtgccttctc cttcaaattc gatgacgcgg tcttcgggag atcctctcag gacgtaggta
     7981 tcgtctcccc tgccgccttc catcgtgtcg gcgccctcgc caccgtcgag ccgattgtcg
     8041 ccgtcattgc cgaccatccg gttgtcgagc gaattgcccc ggccggctat cgcgcttgcg
     8101 atcaggatga ggttctccac cccgacaggg agccggtaat cgatgctgga gatcaccatg
     8161 tcggcaaagc cctcgccgcg gaactcggcg acgcgatcat tctcgtcgtc gacccagtaa
     8221 gtatcgtcgc cgaagcggcc gaacatctca tcggcgccct cgcccccgtc gagctcgtca
     8281 ttgccgcgca ggccgtaaag cttgtcgctg ccgccgaagc cgctgaggct gtcggattcg
     8341 cgggtgccga tcagcacatt gtcgccctcg tcgcccttga tcaccgccat gacagaatcc
     8401 ccactatgaa gtgtttccga aaagactttt cgcgcgcggc ccgcgcgacg cgagacggct
     8461 tagtcgaagc cgacgaaatc ggtgtgatcg atcactgtcc caggggtcaa tatcgcgagg
     8521 aacatcccga aactgcctcc gaaaccgtcc gggtcgtact tcacctggcc ggtgtctgga
     8581 tcgtagatga tccggtcgtc cgcatcctgc gcctgcgtgc ccacgacgaa ggcgttcgga
     8641 tcgagcgtac cctccccgat ttgtttgaag gcgtcccgat cgaggaaaat cgtgtcgtcg
     8701 accgatgaga agtctttgat gatgtcagcg atgtagaagc cgtattggac atcttcgaaa
     8761 tagaagccgt cattgcccgc tccgcccacc agcttgtcgt cggatgagcg ctggccgtag
     8821 aggctgtcat caccgccgcc gccgatcagg gtatccgatc ccaaatcccc cttgagaaca
     8881 ttgtcggcat cattgccgac gatcctgttg ccggcaatgt tgccggtgcc gttgatcgca
     8941 gccgacccgg tgaggaccat atcctcgaca aaaccgctat aatcgaaagc gagggtgtag
     9001 ctgacgctgg cgcggaccag gtcgatcttg ttgtcgaagg tgttcttttc gtcgatcgtg
     9061 tcgttccgat gatcgacgat gtaggtgtcg gcgccgtagc tgccgctcat gaagtcggcg
     9121 ccgcccccgc catcgaggac attgtcgatc accgcggtct gcgcgaagat ctgattggcg
     9181 gttgcgctgc cccaggcgtg cgtggcgttg ccggtcagac tcagaacctc gatttcgccg
     9241 tacaggcgga tcgaggccga ggaaatgacc gtgtccctcc cttcgccgcg gcgctcggtg
     9301 acgcggtccc cgaggtgatc cgcatggtag gtgtcgtccc cgccgccgcc gtacatcagg
     9361 tcggcgccgg cggcgccgtt gaggacattg gcggcttcgt ttccgtagag ccggttggcg
     9421 gcagcgttgc cgttgcccga cacggccgcc ccggtcagcc gcagaatctc gaaatgctcg
     9481 gccaagctgt ggctgaggct gctgatgacg gtgtcttcgc cctcgtcggc ccgctcgatg
     9541 cgcgtctcgc cattctcggt cagataatag acatcgttgc cgagaccgcc ttcgagaata
     9601 tcggctccga ggccgccgtc gaggcgattg tcgcccgagt tgccgacgat gtgattctcg
     9661 taatcgttgc cgcggccgac cagggaatcg gtgccgatca gcaccagatt ctcgatattc
     9721 ttctgcagct tgaagctcac cgaagaaaat acgacgtcgg ccagtccctc gttgcggaac
     9781 tcgcgcacca catcgccgat atcgtcaacc catcagtatc gtcgccgaag cggccgacca
     9841 tcaggtcggc gccttcgccg ccgtcgagat cgtcgttgcc gcgcaggccg taaagccggt
     9901 cgtcgccgcc gaagccgctg aggctgtcgg attcgcgcgt gccgatcagc acgttgtcgc
     9961 ccgagtcgcc cttgatcact gccatggcct tgcactcctc tggtccggcc tccaatcaag
    10021 gaccgtttcg gtgcagagtc aatcaccttg acggcgaagc ttgcattccg gcgcggcccg
    10081 gctaggccgg ctttttggaa ggagagggac gtgagactgc tcgcttcctg ggccgcgctg
    10141 gcggtcttgt ccgcggccgc gcccgccgcc gcccagcccg atcatgccgc gcgcctgaag
    10201 gaaggcgagg tggtgcttcg cgactttcgc ttcgcttcgg gcgagacgct gcccgagctt
    10261 cggatccatt atgcgacgct gggatcgccg cggcgggacg cgagcgggcg gatcgccaat
    10321 gcggtgatgg tgctgcacgg caccggcggc tcgggccggc agttcctgca gccgcaattc
    10381 gccggcgagc tgttcgggcc gggccagccg ctcgacctgc gccgatatta cgtgatcctg
    10441 cccgacaata tcggccacgg gaaatcgtcc aagccgagcg acgggctgcg catggcgttc
    10501 cccaaatatg attatgacga catggtcgag gcgcagcggc ggatgctggc cgaggggctg
    10561 aaggtcggct cgctgcggct gctgatggga acgtcgatgg gctgcatgca cggcttcgtg
    10621 tgggcggccc ggcacccggg gttcgcgcgc gcgatgatgc cgatggcgtg cctgccggtc
    10681 gagattgccg ggcacaaccg gatgtggcgc aaggccgcgg tcgaaggcat cacgagcgac
    10741 ccggcctggg cgggcggaaa ttaccggacc cagcccgtgc agggcctgcg caccgcggtg
    10801 agcctgctcc aggtcgccgg cttcgcgccg ctctacctcc aaaaggccta tccgacccgc
    10861 gccgccgccg acgcctatat cgtcgagcgg atcgacaagg atttgcccag ccgcgacgcc
    10921 aacgacctca tctaccagct cgaggcctcg cgcacctaca atccgtggcc gaccctggag
    10981 cggatcaatg tgccgatgac gtggatcaat tcgagcgacg atttcatcaa cccggccgaa
    11041 tatggcatcg ccgaggacgc cgcccgccgg atgccgagcg tccgctacat cctggtccgc
    11101 gccaccagcg acacacgcgg ccacggcacg cacacatggg ccaaattctg gcgccaggag
    11161 ctgagcgacc tgctggcgcg gacggagcgt gacgatccgt gacaagcgct tggttccgtg
    11221 acactcacgg ggggtgatga agctcctcgc ctcgctcgcg gcgatgccgc tccttctgct
    11281 ggccccgatt ggcgccgacg ccgccgcccc gaaggaggga cgctttccgc cactcgggga
    11341 ctgccgcgac gaccgcgggg tcgaccgctg cgacccggcg cagcatcgac gcgtgcttgc
    11401 gctatacggc gttcagccga tcgaggccca tctggcagcc ggcgatatcg tgcgccgtgc
    11461 ctttttcgtg gatggctacg gcagcgatcg tgtcgcgatc agctttgtcc gcgccaaggg
    11521 ccgggatccc gcgctttggg tgcacgtccc ctcggggaag gagcagggcc gcgcccccat
    11581 gcaggcgccg gttccagggc cggtatggga cgatttgatc aggcgatcgg cccatttcga
    11641 caggacgctc gcgccgccga agccgggtcc tgatggcgag gaggaggtcc tctgtattca
    11701 cagctgggtc tacacgctcg aagccaccga gcttcccgac gaactggccg ggcaacccgg
    11761 ccgcgtccgc cgcaagaccg aggatgcctg cgacgacggg ctcgcggagg cctacgcgct
    11821 cgagcttgca agggccgccg tgccgctgct ccccgcctgt gcattgatcg atccgcggca
    11881 gcagcgcggc ctccctgggg tgctgagcgc gtgctttttg ctgaccggcg accggctggc
    11941 ggcggccgag gtcttgaacc ggctcaacct gatccaggcc gccgagaacg agtcggatgc
    12001 agcccgtgca gagcaggttt tcggcccgga ggcgcaactc gactggcaag ggtcgcgcag
    12061 cgccgatcgc gcgcaagccg caaagctctg gttggctaag ctggcggagg ggggcggcgc
    12121 gcaacttttc tacgataagg tcgaggggct ctcgcccgac cgcgtgaggt tcgtcggctt
    12181 cctcagccgg tatgccgggg aagcgaacgg cgagtccgtt aatcagttcg cttcggtcga
    12241 gcagatctgg acgcgggagg gcggtgactt cttcatcgag cgggccattg tcgggccctg
    12301 ggagcgggag cgctagcgcc gcacctcctt cgagctcaca tggttgagcg cgatcaccca
    12361 gcggccatcc actttgcgca tcagccgcgt gttgatgccg tcctgcggac tgccgccacc
    12421 ttcgagccgg tagcggctga tcagttgggc cgcgtcgggc gcgagcagct cgatcctgat
    12481 gtcggagaag cggagcttgc cgcgccgctc gggcgagccg ccatagtcgc ggacataatg
    12541 gtcgagcgtg ccctgccagt ccttctggaa ttcgccgcgc gagacgaaca ccacgtcggg
    12601 cctcttgaag cccgccatat agccgcggaa atcgccgcgg ttccaggccg cctccatgtc
    12661 ggcgatgacc tggcggatcg ccgctttctc ggtttccggc gtcgccgcag cggcctctgc
    12721 cgaaggcgcg gagcaggcgc agagcgtcgc cagaagggcg agtgaaagcg ctgcgcggga
    12781 ggtcgttttc attggcttgc tccggccgcg aaggagggag cggcgaggcg atactaagcg
    12841 attgcgctgc aacgtaaaac cttcctcggc gggtgaacgg cggatgaacg ggccgttctc
    12901 cctccattca tggagcaggg cgacaattca agcgttcggg gactcggtcc gcgaagcgcg
    12961 ggtcccctgt gtttgggagt tcggctcatg aacaaactga tcggcaaatc catcatcggc
    13021 gcggccgctg cggtcacgct tgcggccacg gcggcccccg ccgaggcgcg ccactggcgc
    13081 cacgggggag acggcgacgc gctcgccgcc gggatcatcg gtctcggcat cggcgccgcg
    13141 atcgcctcgt cccaccatgg cggctattat ggcggcggcc attacggcgg cggctatggc
    13201 ggcggctatt atggccggcc cgcttattac ggggcccccg tttattacgg ccgcccggct
    13261 tattacggcc ggccccagta ccgcccgcat catggctatt acggccgccg ctgcttcacc
    13321 cgccgcgtgt gggatcccta ttggggccgc cgcgtccgcg tccgggtctg ctgacgagcc
    13381 tgaggcccgc cagcagaatg gggtctagcg ggtgagcttc tcgcccgccg cggccttgcg
    13441 cgtcagcagc tcggacaagg tgaagtccgc gccgagccgc tgctgctggc gggttatgcc
    13501 ttcgacgatc ttggcgagcc cctcgctatc ggcccagaac atcgggccgc cgcgatagac
    13561 cggccagcca tagccgtaga cccagaccac atccacgtcg gaggcgcgct gggccatgcc
    13621 ttcctcgagg atcttggcgc cctcgttgac catcgtgtag agcgtgcgct cgacgatctc
    13681 ctgatcgtcg atctcgcgcg cctcgacgcc ttcgcgcgcc cgaaagcctt cgatgatctc
    13741 ctgcaccttg ggggaagggg aggggcggcg cttctcgtca taatcgtaga agccggcgcc
    13801 tttcttctgg ccccagcggt cgattgcgca gagcgcatcg cggatattct cgatccggtt
    13861 gggatcgcgg tgccagccga tgtcgacgcc ggcgaggtcg gccatctgga acggccccat
    13921 cggcattccg aaatcggtgt ggacgcggtc gacctgctcg ggcgtggcgc cttcgagcag
    13981 cagccgcatc gcctgggtct ggcgcgggat cagcatgcgg ttgccgatga agccgtagca
    14041 gacgcccgcc accaccgcga ccttgcggat cttcctggcg agctgcatcg ccgtcatcag
    14101 cacgtcgggc gcggtcttgg cgccgcgcac gacttccaag agcttcatga tgttggccgg
    14161 cgagaagaag tggaggccga gcacgtcctg cggccgcgag gtgctggccg cgatttcgtc
    14221 gatattgagg taggaggtgt tggaggcgag gatcgcgccc ggcttggcga tcttgtcgag
    14281 gcggccgaac acctccttct tgacgtccat attctcgaac accgcctcga tgatgaggtc
    14341 gcattcggcg agcgcgtcga agtcgagcgt gggattgagc agccccatcg cctgctcgac
    14401 ctggtcggag gtcatccggc ccttggccgc ggtcgcctcg taattcttgc gcatcacgcc
    14461 cgcaccgcgg tcgagcgcgt cctgcgccat ttcgacgatc gtcacgggaa tgccggccga
    14521 caggaagttc atcgaaatgc cgccgcccat cgtgccggcg ccgatcacgc cgacgcgtcg
    14581 gatgtcgcgc ggccgggtgc cttcgggcag gccctcgatc ttggccgcct ggcgctcggc
    14641 gaagaagaaa tattgctggg cccgcgcctg attgccgctc atcagctcca tgaacagctg
    14701 ccgctcgtac atcacgccct gctcgtacgg cttggcgacg gccgcctcga tcgcgcggat
    14761 gttcgcctcg ggcgcttcga agccgcggaa cttgcgggcg ttcgccttgc ggaattcgcg
    14821 gaagatgtcg gggttcgaac gtgcctcggc gagcttgtcg tcgcgctcgc tactcttggg
    14881 cagcggccgc acgtccttca cttcatgcgc ataggcgacc gcatgctgct cgaggtcgcc
    14941 ctcgatcaac ttgtcgagca ggccgatctc ataggccttt tgcgcgcgga cgggattgcc
    15001 ggtcgcggcc atttcgagtg cggccgtgac tccggcgacg cgcggcagcc gctgggtgcc
    15061 gcccgcgccc ggcagcaggc cgagcttcac ttcgggaacg cccatggtcg cgttcggcac
    15121 cgccacccga taatggcagg cgagcgccac ttcgaggccg ccgccgaggg cggtgccgtg
    15181 gatcgccgcg accaccggct tcgccgaggc ctcgatgcgg tcgaccacct gcggcagcca
    15241 cgggtgctgc atcggcttgc cgaattcggt aatgtcggcg ccggcgaaaa aggtgttgcc
    15301 ttcgcacagg atcaccaccg cctgcaccgc ctcgtccgct tcggcctggt cgatcgcctt
    15361 caccagcccc tcgcgcaccg agtggctgag cgcgttgacc ggcgggttgt tggacgtgac
    15421 gataaggacg tcgccgtggc ggcgggtggt gatcgggctc atgtaggtct ccagtcagat
    15481 ggccgtgcgg ccgtagcttt gggtgttgaa cggaagcgag gaagcgaggc cgccgtcgac
    15541 cacgagggca tggccgttga cgtagctcga ttcctcggaa gccaggaaga gggcggcggc
    15601 ggcgatctcg ccgggcgaac cgccgcgctt cagcggattc aaatggccga gccgatcctc
    15661 ctgccccttg gcgcgggcgc gctcgtagac gaattcggtc atgccggtct cgatcaggcc
    15721 ggggcagatg gcgttgaccc tgacgttgga gccgctgagc tgctgggcgg aggtcttgac
    15781 caggctgatc acgcccgcct tggacgccga ataagccggt ccgccggcgc ccgagcgcag
    15841 cccggcgacg ctcgcggtgc agatgatcga cccgccgccg cgttccctga tcagcggcgc
    15901 cgcgtgccgg atggcgagcg ccgggccgat caggttgacg cgcaatatct ccgcccaatc
    15961 ctccggcccc tgctccagga tcgaggcgag gccgcccgag acgccggcgt tggcaaagac
    16021 gatgtcgagg ccgccgaacc gcgccgccgc gctccgcacc aggtgggcga catcctcgtc
    16081 gctccccgca tcgccgcgga ccgcgaccgc ggccgggccg atcagcgccg ccgtctcctc
    16141 gaccgcttcc gatatgtcgg agacgaccac gaacgcgcct tccccggcga acagggcggc
    16201 ggcggcccgg ccgatgccgg agcccgcgcc ggtgacgatc gcgaccttgc cttggaggcg
    16261 acccattacg ccctcccgtc attcccgcgg ctgatgagcc tgcaggaaga aaggagaagc
    16321 gggattcccg ctttcgcggg aatgacgagg ggttggtcgc tgtcccgcgt tttgtcgctc
    16381 acagcccgac cccgatcgtc gagccgccgt cgacgacgat cgtctgaccg gtcatgaagg
    16441 tcgaagcggg cgaggcgagg aacaccgcgg cgccggcgat ttcgtgcggc tcgccgatcc
    16501 ggcccagcgg cgtcccccgc gtcaccgcct tcagcgtgtc cggattctcc cacaaggcgc
    16561 gcgcgaaatc ggtgcgcacc agccccggcg cgatgcaatt gacccggacg ccggccgggc
    16621 cgaattcggc ggcgaggttg cgggccagct ggaagtcggc cgccttcgag atattgtacg
    16681 ccccgatcat cgtcgatccg gtcatcccgc cgatcgagga gacgatgatg atcgaccctt
    16741 ccttgcgttc gatcatctcg ggcgccgcca tcgcgatcag ccaatggttg gcgatgacgt
    16801 tattctcgag gatcttgcgg aactgctcgt ccgcgattcc ggccatcggc ccgtaataag
    16861 ggttggacgc cgcgttgcag acgagcacgt cgatacgtcc cagccgccgc cgcgtctcgt
    16921 cgaccagatt ctgcaaatcc tgctttgagg aaatattagc tggaacaact attgcagtgc
    16981 cgtcgccgtg gcactcattg atcgcctggg cggcctcttc acaggcctcg gccttgcggc
    17041 tcgagatcac caccttggcg ccgtgctccg ccatcgcctc ggcgatcgcg cgcccgatcc
    17101 cgcgcgagga accggtgatg acggccactt tgccgctaag gtcgaacagg ttcatctcaa
    17161 ttcctcccct ccttttcgag gggaggggga ccagccaagg gctggtggag gggccgccgc
    17221 gcagcggcgc cttcggcgcc ggcccctcca ccatgcgaag catgcttggc gaactccagc
    17281 cttgcgatgg cgcggttgtg gacttcgtcg gggccgtcgg cgagccgcag ggtgcggatc
    17341 ccggcccagc tataagcgag gccgaaatcc tggctgacgc cggcgccgcc atgcgcctgg
    17401 acggcgtcgt cgatgatcct gagcgccatg ttcggcgcct gcaccttgat catcgcgatc
    17461 tcggcctgcg ccgccttgtt gccggccttg tccatgaggt cggcggcctt gaggcacaac
    17521 agccgcgaca tgtcgatgtc gatccgcgcc cgcgccaccc tttgctccca gatcgaatgc
    17581 tccatcagct tcttgccgaa cgcgaccctt gtctgcagcc gccgcaccat cgccgccagc
    17641 gcctcctcgg ccgcgccgat ggtgcgcatg caatgatgga tccgcccggg cccgagccgc
    17701 ccctgcgcga tctcgaagcc gcggccttcg ccgagcagca tgttggcggc cgggatgcgc
    17761 acgtccttga gctcgatctc catatggccg tgcggcgcgt cgtcatagcc gtagacggtc
    17821 agcgcgcgct cgacggtgac gccgggcgcg tccagcggca ccagcaccat cgactgctgc
    17881 cggtggcggt gggcgccggt gtcggtcttg cccatcacga tcgcgacctt gcagcgcgga
    17941 tcgcccgcgc ccgacgacca ccatttgcgg ccgttcagca cataatcttc gccgtcgcgc
    18001 cggatctcgg tttcgatatt ggtcgcgtcg gacgaggcga ccgcgggctc ggtcatcaga
    18061 aaggccgagc ggatctcgcc gttcatcagc ggccgcagcc attcgtcctt ctgctcgcgc
    18121 gtgccgtagc ggtggagcac ttccatattg ccggtgtcgg gcgccgagca gttgaacact
    18181 tcggacgccc acaggatgcg gcccatctcc tcggcgcaca atgcatattc gaggttggtg
    18241 agctgcgtcc cttcgaattc gaaactctcg tcgacatggg cgagcgcgtg gccgggcggc
    18301 atgaacaggt tccacaggcc cgccgccttc gccgccggct tcagctcctc gatcacctcg
    18361 accggctgcc agcggtcgcc ggcggcgagc tgcgccttat agtcgccggt gcgcgggcga
    18421 atccgagtct cgacgaagtc gcgcacgcgg tcgcggtaaa aagcctgccg ttccgtaagg
    18481 tcgaagtcca tgcggccgtt ttcctcccgt ttcgctcccg ctcctcctgt cagaaagcgc
    18541 ggtttcgcgc caccccgcgc ggcctcgtcc cgccggcggc ggctttccgg gagccgatgc
    18601 ctgtgcggct acatagacgc ggatatgttc atcggctcag gcgcgctccg ggaggggccg
    18661 gttgacgatt cctctattgc cgggcacccc gggggcgagg agagcgcgat gaacccgtct
    18721 gtcgatcacg acaccttccg cttcgaaacc cgcggctggc tcgaggccaa ttgcccgccg
    18781 gaaatgcgtc agccgatgcg ggacgagggc gacgcctgct ggggcggccg caatccggtc
    18841 ttccagtccg aggcgcagaa aaattggctc gatcgcatgg ccgcgcgcgg ctggaccgtc
    18901 cccgactggc cgagcgacta tggcggcggc ggcctctcgc ccgcccagac caagatcctc
    18961 cgccaggaaa tgaccgcgat cggcgcccgc aatccgctct acagcttcgg catttcgatg
    19021 ctcggcccgg cgttgctcaa atatggcagc gaggagcaga agcggcgctt cctgcccgag
    19081 atcgcccgcg gcgagatacg ctggtgccag ggttattcgg agcccaatgc cggctccgat
    19141 ctcgcctcgc ttcagaccag atgcgaggac aaaggcgacc attggctggt caacggccag
    19201 aaggtgtgga ccagctatgc cgacaaggcc gactggatct tctgcctcgt ccgcaccgat
    19261 ttcgaggcgc ccaagcataa aggcatcagc ttcctgctgt tcgacatggc gagcccgggc
    19321 gtgtcgacgc ggccgatcct gctcatctcc ggctcctcgc ctttctgcga gaccttcttc
    19381 gacgacgtga gggtgcccaa ggaccaggtg gtcggtgagg tccacaaggg ctgggacgtc
    19441 gccaaatatc tgcttggcca cgagcgcgag atgatcagcg gcatgggtct cggcgggcag
    19501 ggcggcagcc tcggcgccgc gctcggggag cggctcgccg acgatccgat cctgcgcgcc
    19561 gacgtcgccc gcttcgacgt cgacgcgatg gccttccgcg ccatgtccga acgcttcctc
    19621 gacgagctca aggcgggtca ggcgcatccc gctcagccgt cgatgatgaa atatgccggc
    19681 accgagctca acaaggcgcg ccacgagctg atcatggccg ccggcggctc gaacgcgctc
    19741 gaatgggaaa cggagcggtc gaacggcggc aaggcggcgc gcgaatggct ccgcaccaag
    19801 gcgaattcga tcgaaggcgg caccagcgaa atccagctcg gcatcatcgc caagcatatt
    19861 ctgcagctcc ccggggcctg atcgatgccg ctttacctta ccgaagatca ggtcatgctg
    19921 cgcgacacag cgcgcgactt catggcctcc gagggtgcga tcgcgacgca gctgcgcaaa
    19981 tttcgcggta tgaactgcaa ggacgggttc ggccatgcgc tgtggaggca gttcggcgag
    20041 atgggcttca ctggcatcct ggtggacgaa gcggacggcg ggctcgggct cggccatgtc
    20101 gaggccggga tcgtgctgga ggagatcggg cgcaatctga cgccatcgcc gttcctgacg
    20161 accgcggtgg cagtggtgac cgcattgcgt ggcgcgggcg aggagcttcg agcgcgctgg
    20221 ctgcccggaa ttcttgcagg cgaaacgatt gcggcgttgg cggtcgatga gggacccaaa
    20281 caca
//
LOCUS       DASVRK010000033         8578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_440625, whole genome shotgun sequence.
ACCESSION   DASVRK010000033 DASVRK010000000
VERSION     DASVRK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440625"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VF589_01160"
     CDS             complement(<1..149)
                     /locus_tag="VF589_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEX8256213.1"
                     /translation="MVRPRTETFDKKKVVAENRRARFDYAIESTLEAGIALTGTEVKS
                     LRFGEG"
     gene            complement(268..1143)
                     /gene="dapA"
                     /locus_tag="VF589_01165"
     CDS             complement(268..1143)
                     /gene="dapA"
                     /locus_tag="VF589_01165"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX8256214.1"
                     /translation="MFRGSIPALVTPFRDEAFDEPAFRDFVEWQINEGSHGLVPCGTT
                     GESATLDAAEHRRVVAACVDQAAARVPVIAGCGSNSTAVAKQLMASSAEAGADAALIV
                     LPYYNRPNQDGLHAHFAALAEASPLPIIVYNVPGRTVTDILPETLGRVAALPTVVGIK
                     DASGDLGRVTAHRRLCGPDFVQLSGNDDIALGFLAMGGAGTISVTANVAPRLFAQFHD
                     AWDAGDRETVLSIQDRLFPLHKALFSDASPGPAKYALSRLRPGFPTELRLPMTPPSEA
                     SRKAVDAALQHAGLL"
     gene            1236..3395
                     /locus_tag="VF589_01170"
     CDS             1236..3395
                     /locus_tag="VF589_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8256215.1"
                     /translation="MMSSMQNVRVTLDRVRSSLSRGWSTLDGLWDKAGALGVRRKAVT
                     LGLLAASAAAVGATLAPNAAIRVTPPVQTAQYQPQYQQPPRYQAPPRPAAPMMATPRI
                     SSALARWNSLRQSDNHPFSSYASFLASYRGWPGETGMRKTAERAIDPNGTRAGEVTSY
                     FRLMPPLTATGHARHAFALMAEGNVPAARDAARQAWYGGVLPITDESRLLSQFGSTFT
                     PQDHDRRVEALLDNGDVTSARRTLAYASPGRRAVYEARLALQGKSPDAQSRLAMIGSS
                     AQTDAGVIMDRANWLRDTHQSAAARSLLGQRRNLVTRPRDAEKWYETLLAMARGAAND
                     RNWMTAYQIASQVDDAYPPGTDVSRQSTGERDDYTSLTWLAGTTAFHRLNRPTEAAEM
                     FARYARGGRSSQVLSKGYYWAGRAAQASAQPAQAASYFEQAARHPELFYGQLALERLG
                     RPVPAPLNVAAYMPTANDRAAFQRRDLVEAVRLLGQMGSWEDQSVFVRALAEQAESER
                     DRVLTAELSRQINRPDLGVWLARNARNAGSPFYVRAGYPEISIPPAQSQYWSLAHGIT
                     RQESSFDRAAVSHAGARGMMQLMPGTARETTGKLGLAYDLGRLTRDPTYNIMLGSHYF
                     ARLMDQWGGYAPLAVASYNAGAGNVRKWIREYGDPRTPGVDIVRWIEDIPFSETRGYV
                     QRVLENAVVYDTINPQRARSPAQARLSYYLGKGNRPG"
     gene            3577..4068
                     /gene="greB"
                     /locus_tag="VF589_01175"
     CDS             3577..4068
                     /gene="greB"
                     /locus_tag="VF589_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX8256216.1"
                     /translation="MIDRSNHITPDGYRRLREEYEALFGGERPKLVETISWAAGNGDR
                     SENGDYIYGRKRLREVDRRINFLSKRMAKANVLDPAAQPDRSRVWFGATVTLADEEDN
                     ERVVTLVGEDEADAGAGRVSWNAPIARAVRGAAVGDLRRVQLPSGEKEFEVVAIAYPE
                     ASG"
     gene            complement(4352..4678)
                     /locus_tag="VF589_01180"
     CDS             complement(4352..4678)
                     /locus_tag="VF589_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256217.1"
                     /translation="MHYFRRALTTLAAAGAFAGLSACTYDDYSYGYDRGYGYAGERPY
                     QGEYRGDGGYGEGYREGPYRDDRYRDDRYGQDPYASDRGYGGEEYYPPERDYPREDYP
                     YPDGGR"
     gene            complement(4749..5528)
                     /locus_tag="VF589_01185"
     CDS             complement(4749..5528)
                     /locus_tag="VF589_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HEX8256218.1"
                     /translation="MSVRLSDYESGAKYRGDYAEDLEALQQRLARILVAHIVHRRRSV
                     VVVEGWDAAGKGGAIKRLTAEWDPRSFAVWPIGAPTAEEKGRHFLWRFWTRLPEAGSI
                     AVFDRSWYGRVLVERVEGFAAKAEWRRGYDEINDFEAQQAADGTAIVKLFFHVTQKVQ
                     DARLKARLDHPWKRWKVTEEDFRNRARRPEYLAAIAEMLARTDTAAAPWRVIDGNNKK
                     AARIAALTAVADALEPAVPAEPPEAPPEIAALARAAFGPKA"
     gene            complement(5698..7512)
                     /gene="aspS"
                     /locus_tag="VF589_01190"
     CDS             complement(5698..7512)
                     /gene="aspS"
                     /locus_tag="VF589_01190"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX8256219.1"
                     /translation="MHAYRTHHCAELRADHVGERVKLSGWVHRKRDHGGVLFVDLRDH
                     YGITQIVAKADSEPLRILDRIRAESVVTVVGQVVSRGPEASNPNLATGEIEVAADEVT
                     VQSAAEELPMPVAGEADYPEEIRLRYRYLDLRRERLHHNIVLRSQVISSIRRRMVDQG
                     FTEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQMFKQLIMVAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDLEMSFVTQDDVFAALEPVLGGVFEEFAGGKSVTPAPFP
                     RIPYKEAMLKYGSDKPDLRNPILIADVSDHFRGSGFGLFARIVEGGGVVRAIPAPGTA
                     DKSRKFFDDMNEWARSEGHAGLGYITHKGGELGGPIAKNHGEEATRKLIAELGLGPDD
                     GIFFAAGKEGQAAKLAGLARTRTAEQLGLINESRFDFCWIVDFPMFEYDEEAKKVDFS
                     HNPFSMPQGELEALETKDPLDILAYQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAGY
                     SKDDVDTNFAGMINAFKYGAPPHGGSAPGIDRIVMLLAGEPNIREVVLFPMNQRAEDL
                     MMGAPSEVSAKQLRELNIRLVEPAGAAKKDPAKAVAPE"
     gene            7616..>8578
                     /gene="rnd"
                     /locus_tag="VF589_01195"
     CDS             7616..>8578
                     /gene="rnd"
                     /locus_tag="VF589_01195"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8256220.1"
                     /translation="MLIHPLITDSESLLHLCERLAKSDFVAVDTEFMRENTYWPDLCL
                     IQIGDRQEAAAIDPKAEGIDLTPLLNLLVNNEDVLKVFHAGGQDLEIIYNMTGGTPHP
                     MFDTQVAAMALGLGEQVGYSNLVESLLGTSLDKGARFTDWARRPLDKRQIDYAIGDVT
                     YLARLFPKMLDRLRKTGRGAWLDQEMERLSDPLNYANDPAQAWKKVKIPSRKPEVLGR
                     LKALAAWRELEARGKNLPRGRIMKDETLADIAAHPPAGQESLARVRGLSAAWQGNDIG
                     ARLMDALASAHPLPATEMPARDDRQPGLGKEGALVADLLKLLLKI"
BASE COUNT         1351 a         2826 c         3041 g         1360 t
ORIGIN      
        1 ccctcgccga agcgcagcga cttcacctcg gtgccggtca gcgcgatgcc ggcctcgagc
       61 gtcgattcga tcgcataatc gaaccgcgcc cgccggttct cggcgaccac cttcttcttg
      121 tcaaacgtct cggtgcgggg gcggaccatc agttaagtcc tcccctgctt ttgcagagaa
      181 ggcccgctgc gctcgcgccc ctccaccgcg ctgcgcgcgg tccccctccc ccggaaagcc
      241 gggggaggat ctgtgggtgc atcgccttca cagcagcccc gcgtgctgaa gcgccgcgtc
      301 tacggccttg cgactggctt cggagggggg ggtcatgggg agacggagct cggtggggaa
      361 gcctgggcgg aggcggctca gggcgtattt ggcggggccg ggcgaggcat cgctgaacag
      421 cgccttgtgg agcgggaaca ggcggtcctg gatcgaaagg accgtctcgc ggtcgccggc
      481 gtcccaggcg tcgtggaact gggcgaacag gcgcggggcg acgttggcgg tgacggagat
      541 ggtgccggcg ccgcccatgg cgaggaagcc caaagcgatg tcgtcattgc cggaaagctg
      601 gacgaagtcg gggccgcaga ggcgacgatg ggcggtgacg cggccaagat cgccgctggc
      661 gtccttgatc ccgaccacgg tcgggagcgc ggcgacgcgg ccgagcgttt cggggaggat
      721 gtcggtgacg gtgcggccgg gcacgttgta gacgatgatc ggcagcgggc tcgcttcggc
      781 gagcgccgcg aaatgggcgt ggaggccgtc ctggttgggg cggttatagt aaggaagcac
      841 gatcagggcc gcgtccgctc cggcctcggc ggaggacgcc atgagctgct tggcgacggc
      901 ggtgctgttg gagccgcagc cggcgatcac cggcacccgg gccgccgcct ggtcgacgca
      961 ggcggcgacg acgcggcgat gttcggcggc atcgagcgtg gctgattcgc ccgtcgtccc
     1021 gcacgggaca aggccatgac ttccctcatt tatttgccat tccacgaaat cgcggaaggc
     1081 cggctcgtca aacgcttcgt cgcggaaagg cgtcactaga gccgggatcg acccgcgaaa
     1141 catggttatc tccttcacct tgttgggcaa tcccgacata gctgtcggcg gcgggcgccc
     1201 cggttcattc agcgcctgat aaggaggggg tcggcatgat gtccagcatg cagaatgtac
     1261 gcgtcacact ggacagggtc aggagctcgc tgagccgggg ctggtccacg ctcgatggac
     1321 tttgggacaa ggccggcgcg ctcggcgtgc gcaggaaagc cgtgaccctg ggcctactcg
     1381 cggcctcggc cgccgcggtc ggcgcgacgc tggcgccgaa cgccgcgatc cgcgtgacgc
     1441 cgccggtcca gaccgcgcaa tatcagccgc aatatcagca gccgccgcgc taccaggcgc
     1501 cgccgcggcc ggccgcgccg atgatggcga cgccgcggat cagctcggcg ctggcgcggt
     1561 ggaacagcct ccggcagtcg gacaaccacc ctttctcctc ctacgcctcg ttcctcgcca
     1621 gctatcgcgg ctggccgggc gagaccggca tgcgcaagac cgccgagcgc gcgatcgacc
     1681 ccaatggcac ccgcgcgggt gaggtcacct cctatttccg gctgatgccg ccgctgaccg
     1741 ctaccggcca tgcccgccac gccttcgcgc tgatggccga aggcaatgtg ccggcggcgc
     1801 gcgatgcggc gcggcaggcc tggtatggcg gcgtgctgcc gatcaccgac gagagccggc
     1861 ttttgagcca gttcggctcc accttcacgc cgcaggacca tgaccggcgg gtcgaggcgc
     1921 tgctcgacaa tggcgacgtg accagcgcgc ggcggacgct cgcttacgcc tccccggggc
     1981 ggcgcgccgt ctacgaggcg cggctcgcgc tccagggcaa gtcgcccgac gcgcagagcc
     2041 ggctggcgat gatcggatcg tccgcgcaga ccgacgcggg cgtgatcatg gaccgcgcca
     2101 actggctgcg cgacacccac cagagcgccg cggcgcgatc gctgctgggg caaaggcgca
     2161 atctcgtcac ccggccgcgc gacgccgaga aatggtatga gacgctgctc gccatggcgc
     2221 gcggggcggc caacgaccgc aactggatga cggcctacca gatcgccagc caggtcgacg
     2281 acgcctatcc gcccggcacc gacgtcagcc gccaatcgac cggcgagcgc gacgattata
     2341 ccagcctcac ctggctggcc ggcacgaccg cgttccaccg gctcaaccgc ccgaccgaag
     2401 ccgccgagat gttcgcccgc tacgcgcgcg gcgggcgctc ctcgcaggtg ctcagcaagg
     2461 gctattattg ggccgggcgg gcggcgcagg cgagcgcgca gccggcgcag gcggcgagct
     2521 atttcgagca ggccgcgcgc caccccgaat tgttctacgg ccagctcgcg ctcgagcggc
     2581 tcggccgccc cgtgcccgcg ccattgaacg tcgccgccta tatgcccacg gccaacgacc
     2641 gcgccgcctt ccagcgccgc gacctcgtcg aagcggtgcg gctgctcggc cagatgggca
     2701 gctgggagga ccagtcggtg ttcgtccgcg cgctcgccga gcaggccgag agcgagcgcg
     2761 accgcgtgct caccgccgag ctttcgcggc agatcaaccg gcccgacctc ggcgtctggc
     2821 tcgcccgcaa cgcccgcaac gccggctccc ccttctacgt gcgcgccggc tatcccgaga
     2881 tcagcatccc gcccgcgcag agccaatatt ggtcgctggc gcacggcatc acccgccagg
     2941 aaagctcgtt cgaccgggcc gcggtcagcc atgccggcgc gcgcggcatg atgcagctga
     3001 tgcccggcac cgcccgcgag actaccggca agctcggcct tgcctacgat ctcggccggc
     3061 tgacccgcga cccgacctat aacatcatgc tcggcagcca ttatttcgcg cggctgatgg
     3121 accagtgggg cggttatgcc ccgctcgccg tcgccagcta caatgccggg gccggcaacg
     3181 tgcgcaaatg gatccgcgaa tatggcgacc cgcgcacgcc gggcgtcgac atcgtccgct
     3241 ggatcgagga catccccttc tccgagacgc gcggctatgt gcagcgcgtg ctggagaatg
     3301 cggtggtcta cgacacgatc aacccgcagc gggcgcgcag cccggcccag gcgcggctgt
     3361 cttactatct cggcaagggc aaccggccgg gctgaggcgg cctctcgcca agtggcgatg
     3421 gggactggac cccggccttc gccggggaac agttgttcgc tgcggtggct gccgggcgcg
     3481 agccccggcc aggcgcgcgg gcgcgggcgg gttgtccccg cgtcgatctt cgcgcagcat
     3541 gggctgaacc ttgagaggag ccggacggcg cagacgatga tcgaccgctc caaccacatc
     3601 acgccggacg gctaccgccg gctgcgcgag gaatatgagg cgttgttcgg gggcgagcgg
     3661 ccgaagctgg tcgagacgat cagctgggcg gcgggcaatg gcgaccgctc cgagaatggc
     3721 gactatatct acggccgcaa gcggctgcgc gaggtcgacc gccggatcaa cttcctgtcg
     3781 aagcggatgg ccaaggccaa cgtgctcgac cccgccgccc agcccgaccg cagccgggtg
     3841 tggttcggcg cgacggtgac gctcgccgac gaggaggata atgagcgggt cgtcactctg
     3901 gtcggcgagg acgaggccga tgccggcgcc ggccgggtga gctggaacgc gccgatcgcc
     3961 cgcgcggtcc gcggcgctgc cgtcggggat ttgcggcgcg tgcagctgcc ttctggcgag
     4021 aaggaattcg aggtggtggc gatcgcctat ccggaggcga gcggttagac gcctacgggt
     4081 acgcgttcgt catgccggac ttggtccggc atcccgcttc ttccttggca gcgggtgaag
     4141 tgaagaagcg ggaccccaga tcaagtccgg ggtgacgatt cattgggtct gttcagaacc
     4201 gctcatcctg aggagccgat gagcgaagtc gaagcggcgt ctcgaaggac gggtgcacgg
     4261 tccttcgaga cgggccttcg cctacgctca gtccctcctc aggatgagcg agatttattg
     4321 agcctccgct gacctaacga gctgatcagc tctaccggcc cccgtctgga tagggataat
     4381 cctcgcgcgg ataatcccgc tccgggggat aatattcctc gccgccatag ccgcggtcgc
     4441 tggcataggg gtcctggccg tacctgtcgt cgcgatagcg gtcgtcgcga taagggccct
     4501 cgcgataccc ctcgccatag ccgccgtcgc cgcgatattc gccctgatag gggcgttcgc
     4561 cggcatagcc gtagccgcga tcatagccgt agctgtagtc gtcataggtg caggcggaga
     4621 ggcccgcaaa ggcgcccgcc gccgccaatg tcgtcaaggc gcgccgaaaa tagtgcatcg
     4681 ccattctccc gggaggatcg atgccggttc aacccgcggg cggctcgaaa ggttgccggg
     4741 cggcggggtc aggccttggg gccgaaggcg gcgcgggcga gggcggcgat ttcgggcggg
     4801 gcttcgggcg gttcggccgg caccgccggc tcgagcgcat cggcaacggc ggtgagcgcg
     4861 gcgatgcggg cggccttctt gttgttgccg tcgatgacgc gccacggcgc ggcggcggtg
     4921 tcggtgcggg cgagcatctc cgcgatggcg gcgagatatt cgggccggcg ggcgcggttg
     4981 cggaaatcct cctcggtgac cttccagcgc ttccaggggt ggtcgagccg cgccttcagg
     5041 cgggcgtcct gcaccttctg ggtgacgtgg aagaagagct tgacgatggc ggtgccgtcg
     5101 gcggcctgct gcgcctcgaa atcattgatc tcgtcatagc cgcggcgcca ttcggccttc
     5161 gccgcgaagc cctcgacgcg ctcgaccagc acgcggccgt accagctgcg gtcgaacaca
     5221 gcgatgctgc cggcctcggg cagccgggtc cagaagcgcc acaggaaatg gcggcccttc
     5281 tcctcggcgg tgggggcgcc gatcggccac accgcgaagc tgcgcggatc ccattcggcg
     5341 gtgaggcgct tgatcgcccc gcccttcccc gccgcgtccc agccctcgac gacgacgacg
     5401 ctgcggcggc ggtggacgat atgggcgaca aggatgcggg cgaggcgttg ctggagggct
     5461 tcgaggtcct ccgcatagtc cccgcgatat ttggcgccgg attcgtagtc ggagaggcgg
     5521 acggacattt tagtctcctg aattcctccc ctgcttttca ggggaggggg accatgcgaa
     5581 gcatggtgga ggggcggcgc gaagcgcccc tattccgagg ccgaaggctc gctgcgctcg
     5641 cgcccctcca ccgcacttcg tgcggtcccc ctcccccgga aagccggggg aggaatttca
     5701 ctccggcgcc actgccttgg ctgggtcctt cttcgccgcg cccgcgggct cgaccaaacg
     5761 aatgttgagt tcgcggagct gcttggcgct gacctcgctc ggcgcgccca tcatcaaatc
     5821 ctcggcgcgc tggttcatcg ggaagaggac gacctcgcgg atgttgggct cgccggcgag
     5881 cagcatgacg atccggtcga tgccgggagc cgagccgccg tgcggggggg cgccatattt
     5941 gaaggcgttg atcatgccgg cgaaattggt gtcgacatcg tccttcgaat agccggcgat
     6001 ctcgaacgcc ttgtacatga tgtccgggcg gtggttgcgg atcgcgccgg acgatagctc
     6061 cacgccgttg cagacgatgt catattggta ggcgaggatg tcgagcgggt ccttggtctc
     6121 cagcgcctcg agttcgccct gcggcatcga gaagggattg tggctgaagt cgaccttctt
     6181 cgcctcctcg tcatattcga acatcgggaa gtcgacgatc cagcagaaat cgaagcggct
     6241 ctcgttgatc aggccgagtt gctcggcggt gcgggtgcgg gcgaggccgg cgagcttggc
     6301 cgcctggcct tccttaccgg ccgcgaagaa gatgccgtcg tcggggccga ggccgagttc
     6361 ggctatgagt ttgcgggtgg cctcctcgcc atggttcttg gcgatcgggc cgcccagctc
     6421 gccgcccttg tgggtgatgt agccgaggcc ggcatggcct tccgagcgcg cccattcgtt
     6481 catgtcgtcg aagaatttgc ggctcttgtc ggcggtgccc ggcgccggga tggcgcggac
     6541 gacgccgccg ccttcgacga tgcgggcgaa caggccgaag ccggagccgc ggaaatgatc
     6601 ggagacgtcg gcgatcagga tcgggttgcg aaggtccggc ttgtcggagc cgtatttcag
     6661 catcgcctcc ttatagggga tgcgcgggaa cggcgccggg gtgacactct tgccgccggc
     6721 gaattcctcg aacacgccgc cgagcaccgg ctccagcgcc gcgaacacgt cgtcctgggt
     6781 gacgaagctc atttcgaggt cgagctggta gaattcgccc ggcgagcggt cggcgcgggc
     6841 gtcctcgtcg cggaagcagg gggcgatctg gaaatagcgg tcgaagccgg cgaccatgat
     6901 caactgcttg aacatctgcg gcgcctgcgg cagcgcgtag aatttgcccg gatggacgcg
     6961 gctcggcacc agatagtcgc gcgcgccctc ggggctggag gcggtcagga tcggggtttg
     7021 gaactcggtg aacccttggt cgaccatgcg gcggcggatc gagctgatga cctgcgagcg
     7081 cagcacgata ttgtggtgca gccgctcgcg cctgaggtcg agatagcggt aacggaggcg
     7141 gatctcctcg ggataatcgg cctcgccggc gaccggcatc ggcagctcct cggcggccga
     7201 ctggacagtg acttcgtcgg cggcgacctc gatctcaccg gtggcgaggt tggggttgga
     7261 cgcctcgggg ccgcgcgaga cgacctggcc gaccaccgtc accaccgatt ccgcgcggat
     7321 ccggtcgagg atgcggagcg gctcgctgtc cgccttggcg acgatctggg tgatgccata
     7381 atggtcgcgc aggtcgacga acagcacgcc gccatggtcg cgcttgcggt gcacccagcc
     7441 cgaaagcttg acgcgctcgc cgacatggtc cgcgcgaagc tcggcgcaat ggtgggtgcg
     7501 ataggcgtgc atatcggtgt gcttccgctt cgattggggg gaaagtgcgc gcgagcgctt
     7561 cacttggctg aacccgcttt gtcaagcggg gaggccgccg ctatggaggg cggcaatgct
     7621 gatccacccg ctcatcaccg acagcgaatc gctgctccat ctttgcgagc gcctcgccaa
     7681 atccgacttc gtcgcggtcg acaccgagtt catgcgcgag aacacctatt ggcccgacct
     7741 gtgcctgatc cagatcggcg accgccagga ggcggccgcg atcgacccca aggccgaggg
     7801 catcgacctg acgccgctcc tcaacctgct ggtcaataac gaggacgtgc tcaaggtctt
     7861 ccatgccggc gggcaggatc tcgagatcat ctacaatatg acgggcggca cgccgcaccc
     7921 gatgttcgac acccaggtgg cggcgatggc gctggggctg ggcgagcagg tcggctattc
     7981 gaacctggtc gagagcctgc tcggcaccag cctcgacaag ggcgcgcgct tcaccgactg
     8041 ggcgcgccgc ccgctcgaca agcgccagat cgactatgcg atcggcgacg tcacctatct
     8101 cgcgcggctg ttcccgaaga tgctcgaccg gctgcgcaag accgggcgcg gcgcctggct
     8161 cgaccaggag atggagcggc tgtccgaccc gttgaattac gccaacgacc cggcgcaggc
     8221 gtggaagaag gtcaagatcc cgagccgcaa gcccgaagtg ctggggcggc tgaaggcgct
     8281 ggcggcgtgg cgcgagctcg aggcgcgagg caagaatctg ccgcgcgggc ggatcatgaa
     8341 ggacgagacg ctcgccgaca tcgccgctca tccgccggcc ggccaggaga gccttgcccg
     8401 ggtgcgcgga ctgtcggcgg cttggcaggg gaacgatatc ggggcgcggc tgatggacgc
     8461 gctggcgagt gctcacccct tgcccgccac ggaaatgcct gcccgcgacg atcgccaacc
     8521 cggtctcggc aaagagggtg ctttggtcgc cgatcttctg aagctgctgc tcaagatc
//
LOCUS       DASVRK010000034         7088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_564987, whole genome shotgun sequence.
ACCESSION   DASVRK010000034 DASVRK010000000
VERSION     DASVRK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564987"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..492
                     /locus_tag="VF589_01200"
     CDS             34..492
                     /locus_tag="VF589_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8256221.1"
                     /translation="MILSAGIILYHEGAGALRVLLVHPGGPYWRNKDAGAWMIPKGLV
                     EPGEEPAAAARREFEEELGTRPEGELKPLGRVRQAGGKWVEAFALEGDLDAEAIVSNA
                     FTMEYPPRSGRLRSFPEVDRAAWFTLPEARAMILPSQLPILDWLEEAVAA"
     gene            complement(499..1128)
                     /locus_tag="VF589_01205"
     CDS             complement(499..1128)
                     /locus_tag="VF589_01205"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX8256222.1"
                     /translation="MDSTDAAELIAELQEDKAEHQAAETFRNRAALLIAFLAAMLAIG
                     GLGGGNATDDLLVNNIRANDAWAFYQAKTIRQTLFTIAADDIDASLAAAPPEARAAAA
                     KRVADYRATIARYDSEPDPAAPGDPLRGEGKKELRARALAFEEARDRASAQDNNFDLA
                     EVALQLALVLGSVAILAVNRWILGLSVGLGLIGSVLTANGFLLLFPLPI"
     gene            1265..2908
                     /locus_tag="VF589_01210"
     CDS             1265..2908
                     /locus_tag="VF589_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8256223.1"
                     /translation="MIRNSFARYGALALLLAAAAPSAAQQMPPPNPPRLIVAISVDQF
                     SADLFAQYRQHFTGGLRRLSEGVVFPSGYQAHAATETCPGHSTILTGSRPARTGIVAN
                     SWFDLGAAREDKAIYCAEDERAPGSSSEDYEVSAYHLRVPALGDHMKRANPASRVAVA
                     AGKDRAAVMMGGHRPDQRWWWSGKLFAGEAKTAPAAVAAVNANVAAALAAPRPPLALP
                     PVCAPRARAIPVEGGGKPVGAGRFERPEGDSRLFRASPELDGATLALAAGLRAEMRLG
                     EGAATDLLIVGLSATDYVGHTYGTQGSEMCLQLLELDRSLGDFFARLDATGVDYLVVL
                     TADHGGHDLPERHRQHAAGEAARVDPALAPGTMGKALAAKLSLSGPVLFGDGPFGDFY
                     IDRKLSAAQRARVLAEAVRTYRRHPQVAEIFTAAQIRAAPAPSGPPDRWSLIDRAKAS
                     YDTERSGDFVVLLKPRVTPVFDTSRFYVATHGSPWDYDRRVPILFWRKGLVPFEQSLA
                     VETVDILPTLAVQIGVPIPPGTIDGRCLDLAEGPETSCR"
     gene            complement(3315..4106)
                     /locus_tag="VF589_01215"
     CDS             complement(3315..4106)
                     /locus_tag="VF589_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX8256224.1"
                     /translation="MNLRFVQVDAFADRPFSGNPAAVIPLEREWLPDEMMQAIAAENN
                     LSETAFTIPAAGGEADYELRWFTPTTEVALCGHATLASGHVLIEGPEVRFRTRKAGIL
                     TVARDGDGLTLDLPVTRVKPAAEDRLLAALGTPDAGLFLSYAGAEATAIVLLSDAEAV
                     RACAPDMRALRNVDLMAIVTAPGDGGHDVVSRVFVPAWGVEEDPVTGSAHAALAPFWA
                     ERLGRDRFTAWQASRRGGRIAVRLAGERAILGGRCVTVIEGMFRI"
     gene            complement(4103..5041)
                     /locus_tag="VF589_01220"
     CDS             complement(4103..5041)
                     /locus_tag="VF589_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8256225.1"
                     /translation="MRDNNARLAASAAVASVATAIFLFSLKSFASIQTGSVAMLGSLA
                     DTGLDLLASLVTLYGVRLAATPADHDHRFGHGKAEALAALFQVALITASAALIGWRAT
                     LRLASDQPTAAAEFGIGVSIASIAATFALLAYQRRVIARTGSVAIQADNIHYQSDLFL
                     NLSVIAALVLDQYLGFTGADPVFGIAIALWLLWGAFRASSHAIDQLMDKEWPEEKRQR
                     FLAVAAEHPELRGIHDFRTRRSGSRDFAQFHMYVAPDMSVRDAHDVMEEVEAKIAGEF
                     PGIEVLIHLDPEGHVDQPGNPLVETDVTPIWFGKRP"
     gene            complement(5047..5628)
                     /gene="pdxH"
                     /locus_tag="VF589_01225"
     CDS             complement(5047..5628)
                     /gene="pdxH"
                     /locus_tag="VF589_01225"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX8256226.1"
                     /translation="MADDPHALFDAWFAEARAAEPNDPEAMALATAGADGRPSLRMVL
                     LKGHDARGFTFFTNEQSAKGAELAANPVAALLFHWKSLRRQVRAEGAVERVSGDEAAA
                     YFGTRSRESQIGAWASDQSRPLESRAAFEARYAAMVERFEGQEVPRPPHWGGYRLTPA
                     RIEFWQDRPHRLHERRLFRARPDGGWDEGLLYP"
     gene            5730..6332
                     /locus_tag="VF589_01230"
     CDS             5730..6332
                     /locus_tag="VF589_01230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX8256227.1"
                     /translation="MCNEQRRRVALGQIRDDFSQLRIPLRFPEGMPNLAPLDSIRITD
                     MNAIVRASGEGGAELVQRRWSWLGPGGKPVYNFRSEGRDFGSGRCLIVADGFYEFTAP
                     AAPADGSPKPKLKDKWLFTKTGEDWFCIAGLWRATAEVGEAYTMLTLAPGPDVAPYHS
                     RQVAVLERADWARWLDPAEPAADILRPLPAGSLQVEAVRR"
     gene            complement(6393..>7088)
                     /locus_tag="VF589_01235"
     CDS             complement(6393..>7088)
                     /locus_tag="VF589_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256228.1"
                     /translation="KGGWAVSEWTMLSLRLASLRQLAGDGAGALAAMSRDEAALGKGS
                     DYAVNFRVNRAAYLAEAGRHAEALALVEAVRAAYQAAEDQPIGAGGEKVPGSDRHFQW
                     IRACALKGLGREAEGAAAAAAIFAAEQPTDEYLVVDSNALIRLRLARCTGDAAMAARE
                     IAADARRLPIGGSPFLLLQPALRLGGFDPAFRRRILEHPELAAELRGRFRELPPELVP
                     ALNHWHPPETATP"
BASE COUNT         1064 a         2457 c         2556 g         1011 t
ORIGIN      
        1 tgccgtccgc cgggcggctg acgcgccgcc gggatgatcc tcagcgccgg catcatcctc
       61 taccatgaag gggccggcgc gctgcgcgtg ctgctggtcc atcccggcgg cccctattgg
      121 cgcaacaagg atgccggcgc ctggatgatc cccaagggcc tggtcgagcc cggcgaggag
      181 ccggccgccg ccgcgcgccg cgagttcgag gaggaattgg ggacgcggcc ggagggtgag
      241 ctgaagccgc tcggccgggt tcgccaggcc ggcggcaagt gggtcgaggc gttcgcgctc
      301 gagggcgatc tcgacgccga ggcgatcgtc agcaacgcct tcacgatgga atatccgccg
      361 cgcagcggcc ggctccgctc gtttccggaa gtcgaccgcg cggcctggtt cacgcttccc
      421 gaagcgcgcg ccatgatcct gcccagccag ttgccgatcc tcgactggct cgaggaggcg
      481 gttgccgctt gaggccgctc agatcggcag cgggaagagc agcaggaagc cgttggcggt
      541 gagcacagag ccgatcaggc cgagccccac ggaaagcccc aggatccagc ggttgaccgc
      601 caggatcgcc accgacccga gcaccagagc aagctgcagc gcgacttcgg cgaggtcgaa
      661 attattgtcc tgcgccgagg cgcggtcgcg cgcttcctcg aacgccaagg cgcgggcgcg
      721 aagctccttc ttgccctcgc cgcgcagcgg gtcgccgggc gcggccggat cgggctcgct
      781 gtcgtagcgg gcgatggtgg cgcgataatc cgcaacccgc ttcgcggccg ccgcccgcgc
      841 ttccggcggc gcggcggcca gcgacgcgtc gatatcgtcg gcggcgatcg tgaacagggt
      901 ctggcggatg gtcttggcct ggtagaaggc ccaggcgtcg ttggcgcgga tattgttgac
      961 cagcagatcg tcggtcgcgt tgccgccgcc gaggccgccg atcgccagca tggccgcgag
     1021 gaaggcgatc agcagggccg cgcggttgcg gaacgtctcg gccgcctgat gctcggcctt
     1081 gtcctcctga agctcggcga tgagctcggc cgcgtccgtc gaatccatgt ctgtcccccc
     1141 tcccccggta aagccaatgg ctggcaaatc ccgcgcgcgg tgtcgagagg ggccggctcc
     1201 aaaacaggcg cttgcgcggc ggctttgagc tgttgcacag tcggacgaaa attgggggat
     1261 ggggatgatc cggaacagct tcgcgcgcta cggcgccctt gcgctcttgc tggccgcggc
     1321 ggcgccgtca gccgcgcaac aaatgccgcc gccgaacccg cccaggctca tcgtcgcgat
     1381 ctcggtcgac caattctcgg ccgacctgtt cgcccaatat cgccagcatt tcaccggcgg
     1441 cctcaggcgg ctcagcgaag gcgtggtgtt tccgtcgggc tatcaggcgc atgccgcgac
     1501 cgagacctgc cccggccatt ccaccatcct caccggctcg cgcccagccc gcaccggcat
     1561 cgtcgccaat agctggttcg acctcggcgc cgcccgcgag gacaaggcga tctattgcgc
     1621 cgaggacgag cgcgcgcccg gcagcagctc ggaggattac gaggtctccg cctaccacct
     1681 tcgcgtaccc gcgctgggcg atcatatgaa gcgcgccaac ccggccagcc gggtcgcggt
     1741 ggcggccggc aaggaccggg cggcggtcat gatgggcggc caccgcccgg accagcgctg
     1801 gtggtggagc ggcaagttat tcgccggcga ggccaagacc gcgccggcgg cggtggcggc
     1861 ggtcaacgcc aatgtcgcgg ccgcgctggc ggcgccgcgc ccgccgctgg cgttgccgcc
     1921 ggtctgcgcg ccgcgggcgc gggcgatccc cgtcgagggc ggcggcaaac cggttggcgc
     1981 cggccggttt gagcggcccg agggcgatag ccgcctgttc cgcgcctcgc ccgagctcga
     2041 cggcgcgacg ctggctttgg cggcaggcct tcgcgccgag atgcggctcg gcgagggcgc
     2101 cgcgaccgac ctcctcatcg tcggcctgtc ggcgaccgat tatgtcggcc acacctacgg
     2161 cacccagggc agcgagatgt gcctgcaatt gctcgagctc gaccggtcgc tcggcgattt
     2221 cttcgcgcgt ctcgatgcca ccggcgtcga ttatctggtg gtgctgaccg ccgaccatgg
     2281 cggccacgac ctccccgagc gccaccgcca gcatgccgcc ggcgaggcgg cgcgggtcga
     2341 tccggcgctc gcccccggca cgatgggcaa ggcgctggcg gcgaagctca gcctttccgg
     2401 acctgtgctg ttcggcgacg gcccgttcgg cgacttctat atcgaccgca agctgagcgc
     2461 cgcccagcgg gcgcgggtgc tggcggaggc ggtgcgcacc tatcgccgcc acccgcaagt
     2521 ggccgaaata ttcaccgccg cccagatccg cgccgccccc gccccgtccg ggccgccgga
     2581 ccgctggagc ctgatcgaca gggccaaggc ctcctacgat accgagcggt cgggcgattt
     2641 cgtggtgctg ctgaagccgc gcgtgacgcc ggtgttcgac accagccgct tctacgtcgc
     2701 cacccatggc agcccctggg attacgaccg gcgggtgccg atcctgttct ggcgcaaggg
     2761 gctggtgccg ttcgaacagt cgctggcggt cgagacggtg gacatattgc ccaccctcgc
     2821 cgtccagatc ggggtgccga tcccgcccgg cacgatcgac ggccgctgcc tggatctggc
     2881 cgagggcccg gagaccagct gccggtgagg ggggccacgc gccaaggcaa gatccgcgta
     2941 tgtttgaacc gttcgtcccg agcgaagtcg agggaccctg ccgagcgaag ccgaggccct
     3001 atttcaggga cggttctcag cttcgctcga aagggtgtct cgacttcgct cgacacgaac
     3061 gggtggtggg tgagagcggt gcatcaagac gtgtccggtg acgggaaacg tcagcagttc
     3121 atcgggtcac ttaccacccg ctcatcctga ggagggactg agcgaaggcg aaggcccgtc
     3181 tcgaaggaca agtgtgcgtc cttcgagacg ccgcttcgtc aagctcagcg gctcctcagg
     3241 atgagcgggg aattgatgcg accgcgctac taaatcgggt caggatgagc ggggcgggtg
     3301 atcccacccc cacctcaaat acggaacata ccctcgatca ccgtcacaca ccgccccccc
     3361 aaaatcgcgc gttcccccgc gagccgcacc gcgatccgcc cgccgcgccg gcttgcctgc
     3421 caggctgtga agcggtcgcg gccgaggcgc tcggcccaga aaggcgcaag cgcggcgtgg
     3481 gcggagccgg tgaccgggtc ctcctcgacg ccccaggcgg gcacgaacac ccggctgacc
     3541 acgtcatggc cgccgtcgcc gggggcggtg acgatcgcca tcaggtccac gttgcggagc
     3601 gcgcgcatgt cgggcgcgca ggcccgaacc gcctccgcgt cagaaagcag gacgatcgcc
     3661 gtcgcctcgg cgcccgcata ggacaggaac agcccggcat cgggcgtgcc gagcgcggcc
     3721 agcagccgat cctcggcggc gggcttcacc cgcgtcaccg gcaaatcgag cgtcagcccg
     3781 tcgccgtcgc gggcgacggt gaggatgccg gccttgcggg tacggaagcg cacctccggc
     3841 ccttcgatca gcacgtggcc gctcgcgagc gtggcgtggc cgcacagcgc cacctccgtc
     3901 gttggcgtga accagcgcaa ttcgtaatcg gcctcgccgc ccgccgccgg gatcgtgaag
     3961 gcggtctcgc tgagattatt ctcagccgcg atcgcctgca tcatttcgtc cggcagccat
     4021 tcgcgttcga gcgggatcac cgcggcggga ttgccggaaa aggggcggtc ggcgaaggcg
     4081 tcgacctgga cgaagcggag gttcatgggc gctttccgaa ccagatcggg gtgacgtcgg
     4141 tctcgactag aggattgccg ggctggtcga catggccttc gggatcgagg tggatcagga
     4201 cctcgatgcc ggggaactcg cccgcgatct tggcctcgac ctcctccatc acgtcatggg
     4261 cgtcgcgcac gctcatgtcc ggcgcgacat acatgtggaa ctgggcgaag tcgcgcgagc
     4321 cgctgcggcg ggtgcgaaag tcgtggatgc cgcgcagctc gggatgctcg gcggcgaccg
     4381 cgaggaaacg ctggcgcttc tcctcgggcc attccttgtc catcagctgg tcgatggcgt
     4441 ggctcgacgc gcggaacgcg ccccacagca gccagagcgc gatggcgatt ccgaacaccg
     4501 ggtcggcgcc ggtgaagccg agatactggt cgagcaccag agccgcgatc accgacaggt
     4561 tcaggaacag gtcgctctga taatggatat tgtcggcctg gatggcgacc gatccggtgc
     4621 gcgcgatcac ccggcgctga taggcgagca gggcaaaagt ggcggcgatc gaggctatcg
     4681 aaacgccgat gccgaactcg gccgccgcgg tcggctggtc ggaggcgagg cggagcgtgg
     4741 cgcgccagcc aatcagcgcg gccgacgcgg tgatgagcgc gacctggaac agagcggcga
     4801 gcgcctcggc cttgccgtgg ccgaagcgat gatcgtgatc ggctggggtc gcggcgagcc
     4861 ggacgccgta aagggtgacg agcgaggcga gcaggtcgag cccggtatcg gcgagcgagc
     4921 ccagcatcgc caccgagccg gtctggatgc tggcaaagct cttgagactg aacaggaata
     4981 tcgccgtggc cacgctcgcg acggcggcgc tggcggcaag cctggcatta ttatcgcgca
     5041 gcgcggtcac gggtagagca gcccttcgtc ccagccgccg tcgggccggg cgcggaacag
     5101 ccggcgctcg tgcagccggt gcggccggtc ctgccagaat tcgatccggg cgggggtgag
     5161 ccgatagccg ccccaatgcg gcggccgcgg cacctcctgc ccctcgaacc gctccaccat
     5221 cgcggcgtag cgcgcttcga aggcggcgcg gctctcgagc ggccgcgact ggtccgacgc
     5281 ccaggcgccg atctgcgatt cgcggctccg cgtcccgaaa taggcggccg cctcgtcgcc
     5341 ggagacgcgc tcgaccgcgc cttcggcgcg cacctggcgg cgcagcgact tccagtggaa
     5401 cagcaaggcc gcgaccggat tggcggcgag ctcggcgccc ttcgcgcttt gctcgttggt
     5461 gaagaaggtg aagccgcgcg cgtcatggcc cttcaagagc accatgcgca gcgaaggccg
     5521 gccgtcggcg ccggcggtgg cgagcgccat cgcctcggga tcgttgggtt cggcggcgcg
     5581 cgcttcggcg aaccaggcgt cgaacagggc atggggatcg tcggccatcg cggacagctt
     5641 ttagtcggcg ggacgcgcgg ggtcgagtgc agggggccgg gcgcggcggg atttgcccaa
     5701 gcgcgccgcc tggggctatc gcgggtgcca tgtgcaacga acagcgccgc cgcgtcgcgc
     5761 tcggccagat acgcgacgat ttcagccagc tgcgcatccc gttgcgcttc ccggagggca
     5821 tgccgaacct cgccccgctc gacagcatcc gcatcaccga catgaacgcg atcgtccgcg
     5881 ccagcgggga gggcggcgcc gagctcgtcc agcggcgctg gagctggctc ggcccgggcg
     5941 gcaagccggt ctacaatttc cgctcggagg gccgcgactt cgggagcggc cgctgcctga
     6001 tcgtcgccga cggcttttac gaattcaccg cgcccgccgc tccggcggac gggtcgccca
     6061 agcccaagct caaggacaaa tggctgttca ccaagacggg cgaggactgg ttctgcatcg
     6121 ccggcctgtg gcgggccacg gccgaggtcg gcgaggccta tacgatgctc accctcgcgc
     6181 ccggcccgga cgtcgcgccc tatcacagcc gccaggtggc ggtgttggag cgcgccgact
     6241 gggcgcgctg gctcgacccg gccgagcccg ccgccgacat cctccgcccg ctccccgccg
     6301 gcagccttca ggtcgaggcg gtgaggcggt gagcggggtg ccggatcgag tccggcagga
     6361 cgaggaatga gcggagcgcg ccccgcgcca gctcagggcg tcgcggtttc gggcgggtgc
     6421 cagtggttga gggcggggac cagctccggc ggcagctcgc ggaagcggcc gcgcagctcg
     6481 gccgcgagct cgggatgttc gaggatgcgg cggcggaaag ccggatcgaa cccgccaagg
     6541 cgcagcgccg gctgcagcag caagaacggg gagccgccga tcggcagccg gcgcgcgtcg
     6601 gcggcgattt cgcgggcggc catggcagcg tcgccggtgc agcgggcgag gcggaggcgg
     6661 atgagggcgt tcgaatccac caccagatat tcgtcggtcg gctgttcggc ggcgaagatc
     6721 gccgccgccg ccgccgcgcc ttcggcctcg cggccgaggc ccttgagcgc gcaggcccgg
     6781 atccactgga aatggcggtc gctgcccggg accttctcgc cgccggcgcc gatcggctgg
     6841 tcctcggccg cctgataggc ggcgcggacc gcctcgacga gcgccagcgc ctcggcatga
     6901 cggccggctt cagcgagata ggcggcgcgg ttgacccgga agttcaccgc atagtcgctg
     6961 cccttgccga gcgccgcctc gtcccgtgac atggcggcga gcgcgcccgc gccatcgccc
     7021 gccagttgcc ggaggctggc cagccgcagc gagagcatcg tccactcgga gaccgcccag
     7081 ccgccctt
//
LOCUS       DASVRK010000035         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_565053, whole genome shotgun sequence.
ACCESSION   DASVRK010000035 DASVRK010000000
VERSION     DASVRK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565053"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF589_01240"
     CDS             <1..497
                     /locus_tag="VF589_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8256229.1"
                     /translation="AGESGLGVRPYRRTLAQLRIDPPAPADLPLVIDASGRFYFKPEA
                     GGRLWLSPHDETACAPCDTAPDDYDVALAIDRLERVVDWRVERVEHSWAGLRSFAPDR
                     LPVYGFDRHVPGFFWCAGQGGFGIQTAPAGAKLAAALLLGAAPDPMVAGIDPEPYLAG
                     RFTN"
     gene            complement(470..937)
                     /gene="rnhA"
                     /locus_tag="VF589_01245"
     CDS             complement(470..937)
                     /gene="rnhA"
                     /locus_tag="VF589_01245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX8256230.1"
                     /translation="MSAAAVLPKVEIFTDGACKGNPGPGGWGAVIRSGAREKELSGGE
                     PVTTNNRMELLAAISALEALKRPCEVVLTTDSNYVRDGITKWIHGWQRNGWRTSDKKP
                     VKNEELWRALVAAAAPHRIDWRWVKGHAGHSENERADKLACDAALQFVKRPAR"
     gene            complement(940..1890)
                     /gene="ispH"
                     /locus_tag="VF589_01250"
                     /gene_synonym="lytB"
     CDS             complement(940..1890)
                     /gene="ispH"
                     /locus_tag="VF589_01250"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX8256231.1"
                     /translation="MATSTRPLTLLIAAPRGFCAGVDRAIRIVELALERYGAPVYVRH
                     EIVHNKFVVDGLKAKGAIFVADLDQVPDGVPVVFSAHGVPKAVPAKAAERGLNYVDAT
                     CPLVSKVHRQAERLVEHGRHILFIGHAGHPEVIGTLGQVPDGQMTLIETAGDAASVAP
                     ADPHNLAFLTQTTLSVDDTAEIVAILQRRFPDIKGPRGEDICYATSNRQAAVKDIAKR
                     CDAMLVIGAPNSSNSVRLVEVAEREGVPARLIGRAADLDLGWLDGVRTLGITAGASAP
                     EILVRELVDRLALAFDVREEAIEGVQERMVFKLPPGLEAA"
     gene            2022..2660
                     /locus_tag="VF589_01255"
     CDS             2022..2660
                     /locus_tag="VF589_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256232.1"
                     /translation="MINPKAAFASLLSLALVACSGDGEITALGIQTTRSACPAVAIPA
                     ATGDITLFNPPASRDAAALDVVANITNLRSTCNETGQYIVSTASFDVYAQRRNPQGAR
                     EVVVPYFATVVQGGTNVVSKRIGRVALRFEDGQYRASTRGAATGQVLRSAATLPVDIR
                     RKITRDRKPGDVDAAIDPMADPAVRTAVQRASFEFLVGFQLTQEQLAYNATR"
     gene            complement(2727..>3160)
                     /locus_tag="VF589_01260"
     CDS             complement(2727..>3160)
                     /locus_tag="VF589_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256233.1"
                     /translation="MTAYARNAGCSDYLDVDPHDIDVSDLDPAAMRTWTADQWVRACR
                     SEFMAWLERARGGPAPLEGPGAPLCFVFNRDAARAAAEAGYDPTADPKTIRTDDLEPR
                     AIWSWTEDQLARAWQSGLLGRLPRRVWEDLAAVHAAREAAR"
BASE COUNT          451 a         1109 c         1104 g          496 t
ORIGIN      
        1 gggccgggga gtcgggcttg ggcgtccgac cttaccggcg cactctcgct cagctccgaa
       61 tcgatcctcc cgcgccggcc gacctgcctc tggtgatcga cgcaagcggc cgcttctact
      121 tcaagccgga ggcgggcggg cggctgtggc tgagcccgca tgacgagacc gcctgcgcgc
      181 cgtgcgacac ggcgcccgac gactatgacg tcgcgttggc catcgaccgg ctcgagcggg
      241 tcgtcgactg gcgggtcgag cgggtcgagc atagctgggc ggggctgcgc agcttcgcgc
      301 ccgaccggct gccggtctac ggcttcgacc gccacgtgcc gggcttcttc tggtgcgcgg
      361 ggcagggcgg cttcggcatc cagaccgcgc cggccggggc caagctcgcc gccgctttgc
      421 tgctcggcgc agcgcccgat ccaatggtcg cgggcatcga ccccgagcct tacctcgccg
      481 gccgcttcac gaactgaagc gcggcgtcgc aggcgagctt gtcggcgcgt tcattctcgg
      541 aatggccggc atggcccttg acccagcgcc agtcgatccg gtgcggcgcg gcggcggcga
      601 ccagcgcgcg ccatagttcc tcgtttttca cgggcttctt gtcggaggtg cgccagccgt
      661 tgcgctgcca gccgtggatc cacttggtga tgccgtcgcg gacataattg ctgtcggtgg
      721 tcagcacgac ctcgcacggc cgcttcagcg cttcgagcgc gcttatcgcg gcgagcagct
      781 ccatgcggtt gttggtggtg accggctcgc cccccgacag ttccttctcg cgggcgccgg
      841 aacggatcac cgcgccccag ccgcccgggc cgggattgcc cttgcaggcc ccgtcggtga
      901 agatctcgac tttggggagg acggcggcgg cggacaggat caggccgctt ccagccccgg
      961 cgggagcttg aacaccatcc gctcctggac gccctcgatc gcctcctcgc gaacgtcgaa
     1021 ggccaaagcc agtcggtcga ccagctcgcg caccaggatt tcgggagccg aggcgccggc
     1081 ggtaatgccg agcgtccgca ccccgtcgag ccagccgaga tcgagatcgg cggcacggcc
     1141 gatcagccgc gccggcacgc cctcgcgctc ggcgacttcg acgagccgga ccgaattgga
     1201 gctgttgggc gcgccgatca ccagcatcgc gtcgcagcgt ttggcgatgt ccttgacggc
     1261 cgcctggcgg ttcgaggtgg cgtagcaaat atcctcgccg cgcgggccct tgatgtcggg
     1321 gaagcggcgc tggaggatgg cgacgatctc ggcggtgtcg tccaccgaca aggtggtctg
     1381 ggtgaggaag gcgagattgt gcgggtcggc cggcgccacc gaagcggcgt ccccggcggt
     1441 ctcgatcagc gtcatctgcc cgtccggcac ctggccgagc gtgccgatca cttcgggatg
     1501 gccggcatgg ccgatgaaca atatgtggcg gccatgttcg accagccgct cggcctggcg
     1561 atggaccttc gagaccaggg gacaggtggc gtcgacataa ttgaggccgc gctcggccgc
     1621 cttggccggc accgccttgg gcacgccgtg cgcggaaaac accaccggca ccccgtccgg
     1681 cacctgatcg agatcggcga cgaagatcgc gcccttggcc ttgaggccgt cgacgacgaa
     1741 cttgttgtgg acgatctcgt gccggacata gaccggcgcg ccgtagcgct cgagcgcgag
     1801 ttcgacgatg cggatggcgc ggtcgacgcc ggcgcaaaag ccgcgcggcg ccgcgatcag
     1861 cagggtgagg ggcctcgtcg atgtcgccat gggcttcgct ctacggtctg aggggcgccg
     1921 cgtctaccga cgtggctgct tgcagggtgg aaagtcgctt cctatgatcc cgccccacgg
     1981 ctagggcggc tctcgctccc tatgtttgaa ggaccggctt cgtgatcaac cccaaagcgg
     2041 cattcgcctc cctcctgtcg ctcgccctgg tggcgtgcag cggcgacggc gagattaccg
     2101 cgctcggcat ccagacgacg cgctcggcct gcccggcggt cgccattccc gccgccaccg
     2161 gcgacatcac tttgttcaac ccgcccgcca gccgcgacgc cgcggcgctc gacgtggtcg
     2221 cgaacatcac caatttgcgc agcacctgca acgagaccgg ccaatatatc gtctcgaccg
     2281 cgagcttcga cgtctacgcc cagcgccgca atccgcaggg cgcgcgcgag gtggtggtcc
     2341 cctatttcgc cacggtggtc cagggcggca ccaacgtcgt ctcgaagcgg atcgggcggg
     2401 tcgccctgcg cttcgaagac ggccagtatc gggccagcac gcgcggcgcc gccaccggcc
     2461 aggtgctgcg ctcggcggcg acgctgccgg tcgacatccg ccggaagatc acccgcgacc
     2521 gcaagcccgg cgacgtcgat gcggcgatcg atccgatggc cgatcccgcc gtccgcaccg
     2581 cggtccagcg cgccagcttc gaattcctgg tcggcttcca gctcacccag gaacagctcg
     2641 cctacaacgc cacccgctga cccgggctgg ccggcccgcc ggtccggcga aataagttga
     2701 ggaagttgag gcttgccgag agccggtcat cgggccgcct cccgcgcggc gtggacggcg
     2761 gcgagatctt cccagacccg gcgcgggagc cgcccgagga ggccgctctg ccaggcgcgg
     2821 gcgagctggt cctcggtcca gctccagatg gcgcgaggct cgaggtcgtc cgtgcggatc
     2881 gttttcggat cggcggtcgg atcgtagccc gcttcggcgg cggcgcgcgc ggcgtcccga
     2941 ttgaacacga agcataaagg ggcgcccggg ccctcgagag gggccggtcc gccgcgcgcc
     3001 ctctcgagcc aggccatgaa ctcgctccgg caggcgcgca cccactggtc cgccgtccag
     3061 gtgcgcatgg cggcggggtc cagatcgctg acgtcgatgt cgtgcggatc gacgtccaga
     3121 tagtcggaac agccggcgtt acgggcgtag gccgtcattt
//
LOCUS       DASVRK010000036        18246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234 k141_5055,
            whole genome shotgun sequence.
ACCESSION   DASVRK010000036 DASVRK010000000
VERSION     DASVRK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 18246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18246
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5055"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VF589_01265"
     CDS             complement(<1..642)
                     /locus_tag="VF589_01265"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX8256234.1"
                     /translation="MAASSEYLVRFALALLLAFGPAGWSAARAAPEPAGNPALTARVN
                     GVNSAAATSRALRIERLELDVKLHGSIAQTIVTAQFANPGSETLEGNFMLALPAGSVV
                     TGYALDVRGAMADGVLVDQRQGRLAYEARVRAGIDPGLAEVSRDNVFSTRIFPIPPRG
                     GRTIRLRFATPLDPEDGYVLPLRSGDAVGQVALSIEASGIAAAPRLRLPAGLSA"
     gene            complement(712..1536)
                     /locus_tag="VF589_01270"
     CDS             complement(712..1536)
                     /locus_tag="VF589_01270"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX8256235.1"
                     /translation="MSEPRERRPGTLIIIGGHEDRKPDGKRTILREVARHIHGGKLVL
                     ATVASHQPEGYFDEYEKAFADLDTGELVELYVEDRTRAGDRDKLQVIDDAAGIFFSGG
                     DQLRITSQIGDTGIEAKVRALYERGGLIAGTSAGAAAMSDTMLVKGTSGETHRIGDLH
                     MAPGLGLIRDVIIDQHFAERGRFGRLLGAVAHNPRVLGIGIDEDTAAVVHDGHLKVIG
                     SGAVYIVDGEHVTHSNLAEARPDRVLSMHDVCVHVLGTGDSFDIKTRKPVPARSDG"
     gene            complement(1529..2425)
                     /locus_tag="VF589_01275"
     CDS             complement(1529..2425)
                     /locus_tag="VF589_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HEX8256236.1"
                     /translation="MTDSQGRWALIIHGGAKPWDPAEEGANRAGLAQALEAGRRVLAG
                     GGSALDAVEASIRMLEDIAVFNAGHGSVPNAEGEVEMCSGIMDGRDLSAGAVGAIRQV
                     KNPVSVARQLLAEKEVLLVGEGALMFAKERGAPLASDAELLAEEAKKEMEGAHDTVGA
                     VALDAGGNLAAGTSTGGLTGARVGRIGDSPLPGGGLYADNHIGAVSFSGDGETIARLA
                     LASRVMGSLKDGERVGQAIVKAVELLPGTGGAGADGGGIGIAKTGEIGWAHNSEMFAV
                     AVATSDGGEPGIYLKKKEETRV"
     gene            complement(2436..5225)
                     /gene="cphA"
                     /locus_tag="VF589_01280"
     CDS             complement(2436..5225)
                     /gene="cphA"
                     /locus_tag="VF589_01280"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX8256237.1"
                     /translation="MERGALFFGTRSDAATRLLTEPMKVLETGVYRGPHYYSHTPFVR
                     IMVDLGRLEDWPSDRIPGFADRLVEMLPGLDRHGCCFKRRGGFIRRLREGTWIGHVAE
                     HVAIELQTMAGNRVTRGKTRSVKGKPGVYNIMYAYRDERVGLMAGRIALELVNSLLPD
                     DLKGVERLERVYDFQGEFDFGRRLAELKRLVRRTSYGPTTQALIDEAARRRIPVMRLN
                     EQSLIQLGHGRHQQRIRASITGRTSLVAVEIAGDKDLTKKLLAESGIPVPRGVVVRNV
                     EDAVREAKRLRYPLVTKPLDGNHGRGVTIGIMDEEQLRFGFAEAQAQAKGRDIIVEQY
                     FHGHDHRILVVDGKLIAVAERIPAHVIGDGVHTITELIATVNQDPRRGDGHENVMTRI
                     RIDAHVEEYIGRSGLTVDSIPAAGDIVMLRATANLSSGGTAVDRTNEIHPDNAEIARR
                     AAMVVGLDIAGIDFIAPDITRSVRETGGGIVEVNAAPGFRMHLDPSEGAPRDVAKPVM
                     EMLFPRGRSSRVPIFAITGTNGKSTVGRMLKQVLRYTGCTVGLTSTSGVYVNGLLVSE
                     GDATGPRSARLVLRDPTVEVAVLETARGGLLREGLAFDEADIGVVLNVTADHLGLKGI
                     ETLDDLANVKSLIVETVRRGGASVLNADDPQCVRIARRAGGQIIWFSLHGDDEMPPLL
                     RRHLDGGGTAVVREPGEEGGTIVLHQDGRRDFVMKAGDIPATLHGMATFNIANALATV
                     AMCVAHKIPILTIRTALMGFQSSFEQNPGRLNVHDALGFRVIVDYAHNAAGLTALGEV
                     VRGLRHRYKRAIGCISIPGDRRDGDILELGRIAGGIFDELFFREDPSTRGRPRGQIMN
                     LLQEGALQGGASEASIHLIAGEEAATAAALTAANPGELVVITPTEVFAAWQQVVDFQK
                     VESASSPRGQLIAAE"
     gene            complement(5405..6022)
                     /locus_tag="VF589_01285"
     CDS             complement(5405..6022)
                     /locus_tag="VF589_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8256238.1"
                     /translation="MRVRFGHGCGGRGFDIPAAILAAGMGRGGWGRGWGQDEWGAEAG
                     GWGRDSRRRRRFDAGELRLVLLKLIADEPRHGYDLIRAIEELTHGSYAPSPGVIYPTL
                     TMLQDMGLIEEAASEGARKAFAATADGRAHLAERAEELARLMARLTGMGEDRRKAGGG
                     PAGRAVRNLLAALWNRVTEEGADEERLHEIAAILDDAAQRIERLK"
     gene            6506..8845
                     /locus_tag="VF589_01290"
     CDS             6506..8845
                     /locus_tag="VF589_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256239.1"
                     /translation="MADYVIPSHTVSGDRITMGGADTLLVEQGASLSVTANAQAVRFT
                     TPTDGAVITNFGTIEALGDAGDPRAIRFESGIGDTLNATITNASSGSIYARSDAIQIQ
                     AGTLAAGLLTIDNAGSIRSLQSQALDLANSTGTFTVELDNSGTISAEADDAIRFGSIG
                     NVTNEGRIDGGSVDTYSGADGLQYEVNATGTVTNNSGGTIIGDRHGINAGIGSVITVL
                     NSVGGTLEGRNGSGVGSDGTATITNYGSIIGSFSNSVGSDKNGTTVGELDGGGPDGIN
                     DGDGDGIDVDGKATILNGGLIHGVSAGGSGSDGLFNTAEGIAAGGGSIDNLAAGIIRG
                     GGLGILIDDSSQGNAFFLTTVDNKGLIEGLTGTGIRIVSLLADVIVNDGTIKGGNGFA
                     IQFGAGDNRLVIKDGSAMTGIADGQGGVNTLDYRVFGTGGTVVKLATGAATGTGGIDN
                     FVNLLGSAGVDNFAGSAAAELLDGNNGADVLSGGAGNDRLFGDEGNDLLNGGADLDAM
                     AGGLGNDGYSVDNAGDRVTEAFDGGIDRVSSSINHILAANVENLTLVGTAGSGSGNIL
                     ANTITGNAGDNKLDGKGGADRLVGGAGNDGYVVDSSGDRVIEAVAGGTDRVSSSVTHR
                     LAANVENLALTGNEAIDGAGNGDRNGITGNAANNRISGGGGKDVLTGGAGADQFRFDT
                     ALDPVNNWDDILDFSVTDDVIVLDRTIFTGIGTNGTLASSAFVAGTSALDASDRIVFD
                     TATGRIFYDVDGFGGAAQVLIARVDINTPLTHLDFFAVA"
     gene            complement(8946..9536)
                     /locus_tag="VF589_01295"
     CDS             complement(8946..9536)
                     /locus_tag="VF589_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256240.1"
                     /translation="MGHAAGGSGWRVAAWGGAAGMLLLLLLAMQVSKEVQWDAADFVL
                     LGTMLAAAGGGYELAARMSGNWAYRAGAGLALAAAFTLVWINLAVGVIGSEDNPANLV
                     YGGVLGVALVGALAARFRAEGMARAMAATALAQMVAAPIAIAAGLGPLPAGAALELAA
                     LNGCFAAIWLGSGWLFRKAARERGSAPAQGSSSPRT"
     gene            complement(9716..10834)
                     /locus_tag="VF589_01300"
     CDS             complement(9716..10834)
                     /locus_tag="VF589_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8256241.1"
                     /translation="MTTFRLRYVRTRLLVAAGIAIAASAGIWVQNVQAEPPLAEQSLH
                     KVVEEMARSLERYFLDPSAGQAYARELRQRLVSGRYRGLAPDKLAQIVTADLQKIRAD
                     GHLRLSPPSRQPMLGAPSASAAEQQSAETGIDKSGWLADGVAYIGFELFPASKASLAA
                     VREFLETHASARVLIIDVRAHGGGYTDEFDLLASYLYDRPAELIYEDTREAAFRARPD
                     TATLKRLAGPPGFVRQVHLAAPNAAPTGLRGAKLFVLTSGYTGSAAEHLTLALKRTGR
                     ATIIGETTAGMGHFGRVVELPEGFSAFIPIGRPFDPQTGVGWEGIGVKPHVEVPAQKA
                     LETALLQLGLPRQRAQALGAKWMPRGDMERVVPLRGGG"
     gene            10982..11440
                     /locus_tag="VF589_01305"
     CDS             10982..11440
                     /locus_tag="VF589_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8256242.1"
                     /translation="MDALDRALLTILQADSRLTSEQMSERLHLSPSAVNRRVQRLWRE
                     GIIAAEVVVLGEKLRSRLTSAIVNVQLDRHHPSQADELRRILRASPHVQLCLEITGTS
                     DVLLVVSVRDMAHFNAFTDALAQMPLVRRYETHFVKRSIKFTTAISLEED"
     gene            11584..12135
                     /locus_tag="VF589_01310"
     CDS             11584..12135
                     /locus_tag="VF589_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX8256243.1"
                     /translation="MPRLILFNKPYEVLSQFTDSGGGAARSTLSDFVRLPAVYPAGRL
                     DRDSEGLLLLTDDGRLQARIADPKFKLPKTYLVQVEGDAQPDSLERLKRGVQLKDGMT
                     RPAEAERISEPALWPRDPPVRFRRSVPDCWLKLTIREGRNRQVRRMTAAVGHPTLRLV
                     RWSVGGWTIEGLAPGEWREVPVH"
     gene            complement(12148..13299)
                     /locus_tag="VF589_01315"
     CDS             complement(12148..13299)
                     /locus_tag="VF589_01315"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8256244.1"
                     /translation="MQLKQFDARHFLSEHWQKKPLLIRNPWTAWANPLEPDELAGLAC
                     EEEVESRLILTIGNTWKLEHGPFPERRFEQLGDRPWTLLVQSVDRLVPEVAALLPPFR
                     FLPNWRIDDIMVSYATDGGGVGPHFDKYDVFLVQGLGRRRWRIGPDCDASTALRPHDD
                     LRLLPGFEASDDWVLGPGDILYVPPGVAHEGIAVGHDCMTYSIGFRAPSSSELLAGWS
                     EHLLEPLRDDPRYADPDLEEQRNPGEISAEAIARLHAMATARMLDAAAFGRWFGRYNS
                     SPKHPEMDRAREEPIEVDEVRRLIARSLPLRRSPGSRFSFLRQEDGSVLLFADGASLD
                     CDGETARVAEELCGSDCAHVDPALAGSDAVVTLVAELLNQGSVEFGHAD"
     gene            13446..14159
                     /locus_tag="VF589_01320"
     CDS             13446..14159
                     /locus_tag="VF589_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038679.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8256245.1"
                     /translation="MRQFVAFVSLAALAGCQSPPQPRGVEPDETLLSVAASGRVDTRP
                     DEARLQLGVVSVAATSGDAARANREKMDRVAATLARLGVKPDDLQTRNLSLQRIEYGK
                     DRGGYRAENVVEVKLHDMTKVGDAVTAVTEAGANVMSGPDLRVSDPEAASRSAYAAAY
                     KSARSRAEAYAGAAGLKVARVLAIYDGGEGAMPPAYGRGVAMDATAQSVAPPPVSAPF
                     SAGVNTSEVRVRVDFALSD"
     gene            complement(14156..15034)
                     /locus_tag="VF589_01325"
     CDS             complement(14156..15034)
                     /locus_tag="VF589_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8256246.1"
                     /translation="MRLWLFALALAAGSAAFAVPPVPPGAPPLSIGDTHRIVSAPLGE
                     ARVVNVVLPAGYAKAPDKHYPVLYLIDGGIEQDLLHVAGVLHLGAVWGRSGEAIVVGI
                     ETRDRRKELTGPTTDAELLKRYPTAGASAAFRAFIRDEVKPLIRASYRTSGRDAVIGE
                     SLAGLFILETYFDEPALFDAYAAIDPSLWWDGEKLSRAEAAKIDAPQQGRPVYLAMAR
                     EQAEKPAAMKRVTGAIRDAGSPWCLASRPDLLHSTIYQQLTPQALQFLLPPKAPPAPE
                     FGFTVSCSESSSSRAN"
     gene            15133..15480
                     /locus_tag="VF589_01330"
     CDS             15133..15480
                     /locus_tag="VF589_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256247.1"
                     /translation="MARLLSSLLIALGLLLAPLGMANGAAAAMPHHGAAMNMAAADGP
                     CAGMPAPADEKRKADLKLSCASACAAFLPAVAPLAAQAGLAPDPAAPLLPRRLAGLDP
                     ERETPPPRTAPEI"
     gene            15512..15826
                     /locus_tag="VF589_01335"
     CDS             15512..15826
                     /locus_tag="VF589_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256248.1"
                     /translation="MKMLLTVVAMMIASPALAQAAPADAHAAHGGHAAHAASAHAGHG
                     AQDHGQKDDPHAKHQGACCKPDAAGNMPGCCEEKDGKTADCCRESADKKDGQAGHGQG
                     RH"
     gene            15893..17626
                     /locus_tag="VF589_01340"
     CDS             15893..17626
                     /locus_tag="VF589_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HEX8256249.1"
                     /translation="MNQPIGRRDLLRAGALGAAGLGLARLYPAWAQTGTPGLVPQLPT
                     LSGEEIALRIGHTPFAVGGRTGHAITINGTLPGPLLRLREGQRLRLRVDNQLDEDTSI
                     HWHGLLVPFEMDGVPGISFPGIPARGSFTYEFPIVQSGTYWYHSHSGLQEQMGHYGPM
                     IIDPKGADPIASDREHVIVLSDWSFFHPHRIISKLKQEGGFFNRQKETLLGKRSGMSA
                     EDRGMFARMRMDPTDIADVTEAAYTFLVNGHGPGENWTGLFRPGERVRLRIVNAAAQT
                     IFNVRIPGLAMTVVAADGNPVRPVQVEEFQIGNAETYDVIVRPAEDRAYSFVAETIDR
                     SGMARATLAPRLGLTAPVPPLRRRPTLTMKDMGMGGMDHAAMGHGAGHGAAAPAMDHS
                     NMDMRDKTKVSFPVGVGVDMIAPNPVDRTGDPGIGLETVGHKVLTYRDLVSLHPNPDT
                     RPPTRAIEIHLTGNMERFMWSIDGERLSENPEPIRFERGERVRVTMVNDTMMTHPMHI
                     HGHFFEVVNGHAGNHPVKHTMMVLPGGKASFDMTADAPGDWAFHCHLLYHMHAGMMRV
                     VKVRPLAGAAA"
     gene            17623..>18246
                     /locus_tag="VF589_01345"
     CDS             17623..>18246
                     /locus_tag="VF589_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEX8256250.1"
                     /translation="MKHLFLITTFAATFAAPALAQHAGHAPPPAAPADPHAGHNMPAA
                     QPAPPADPHAGHNMPAAEPAAPADPHADHTMPVAEPAAPADPHAGHAMPAAPADPHAA
                     AAPAPDAGPPVAPSPPAALSGPEDAADLVYGRDAMAAARAGMREEHGGMKTYKVLFDQ
                     LEAKLRDGRDGYSWDAQAWYGGDIDKLWVKTEGEGAFGEAVERAEVQA"
BASE COUNT         2914 a         6576 c         5946 g         2810 t
ORIGIN      
        1 cgccgacagg ccggctggga ggcggagccg aggcgcggcc gcgatgccgc tagcctcgat
       61 gctgagcgcg acctgcccga ccgcatcgcc gctccggagc ggcaggacat agccgtcctc
      121 ggggtcgagc ggagtcgcga agcgcaggcg gatcgtccgg ccgccgcgcg gcgggatcgg
      181 gaatatccgc gtgctgaaga cattgtcgcg cgagacctcg gcgaggcccg ggtcgatccc
      241 cgcccggacc cgcgcctcat aggcaagccg gccctggcgc tggtcgacca gcacgccgtc
      301 cgccatcgcg ccgcgcacgt cgagcgcata gccagtcacc accgagcccg ccgggagcgc
      361 gagcatgaaa ttgccttcga gcgtctcgct gccggggttg gcgaactgcg cggtcacgat
      421 ggtctgggcg atgctgccgt ggagcttcac atcgagctcg aggcgctcga tcctgagcgc
      481 ccggctggtg gcggcggcgc tgttgacccc gttgacgcgc gccgtcagtg ccgggttccc
      541 ggcaggctcg ggcgcggctc gggcggcgga ccagccggcc gggccgaacg ccagcaagag
      601 cgcaagcgcg aacctgacca gatattccga cgacgctgcc attccctgac ccccggaccg
      661 ggagcctagg tgcttcagcg cctcaagcaa gccccaaacc aagcggcagc gtcagccgtc
      721 actccgcgcc ggaacgggct tgcgggtctt gatatcgaag ctgtcgccgg tgccgagcac
      781 gtggacgcag acgtcgtgca tcgacagcac ccggtcgggc cgggcctcgg caaggttcga
      841 gtgggtgaca tgctcgccgt cgacgatata gacggcgccg ctgccgatca ccttcaaatg
      901 gccgtcatgg accaccgccg cggtatcttc gtcgatgccg atgccgagca cgcgcggatt
      961 gtgcgcgacc gcgccgagca gccggccgaa gcggccgcgc tcggcgaaat gctggtcgat
     1021 gatgacgtcg cggatgaggc cgaggccggg cgccatgtgg aggtcgccga tgcggtgggt
     1081 ctcgccgctg gtgcccttga ccagcatcgt gtcgctcatc gccgccgccc ccgccgaagt
     1141 tccggcgatg aggccgccgc gctcgtaaag cgcgcgcacc ttggcctcga tgccggtgtc
     1201 gccgatctgg ctggtgatgc gcaattggtc accgcccgaa aagaagatgc cggcggcgtc
     1261 gtcgatcacc tgaagcttgt cgcggtcgcc ggcgcgggtg cggtcctcga cgtagagctc
     1321 gaccagctcg cccgtgtcga ggtcggcgaa cgccttctca tattcgtcga aatagccttc
     1381 cggctggtgg ctggcgacgg tggcgaggac gagcttgccg ccatgtatgt gcctggccac
     1441 ttcgcgcagg atcgtgcgct tgccgtcggg cttgcggtcc tcgtggccgc cgatgatgat
     1501 gagcgtgcct ggccggcgct cgcggggttc agacacgcgt ctcttctttc ttcttgaggt
     1561 agatgccggg ctccccgcca tcgctggtgg cgaccgcgac cgcgaacatc tcgctattgt
     1621 gcgcccagcc gatctcgccg gtcttggcga tgccgatccc gccgccgtcg gcgccggcgc
     1681 cgccggtgcc gggaagcagt tcgaccgcct tgacgatcgc ctgaccaacg cgctcgccgt
     1741 ccttcagcga tcccatcacc cggctcgcca gcgccagccg cgcgatcgtc tcgccgtcgc
     1801 ccgaaaagga gacggcgccg atatggttgt ccgcataaag gccgccgccg ggaagcgggc
     1861 tgtcgccgat ccgcccgacc cgggcgccgg tgagccctcc ggtcgaggtg cccgcggcga
     1921 ggttgccgcc ggcgtcgagc gcgaccgcgc cgaccgtgtc gtgcgcgccc tccatctcct
     1981 ttttcgcttc ctcggcgagc aattccgcgt cgctggcgag cggcgcgccg cgttccttgg
     2041 cgaacatcag cgccccttcc cccaccagca gaacctcctt ttcggcaagc aattgacggg
     2101 ccaccgagac cggatttttc acctgccgga tcgcgccgac cgcgccggcc gaaaggtcgc
     2161 gcccgtccat gatccccgag cacatctcga cttcgccctc ggcattgggg acggagccgt
     2221 gcccggcgtt gaataccgct atgtcctcga gcatgcggat cgacgcctcg accgcatcga
     2281 gcgcgctgcc gccgcccgcg agcacccggc gccctgcttc caacgcctgc gccaggccgg
     2341 cgcggttggc gccctcctcg gccgggtccc agggcttggc gccgccatgg atgatcagtg
     2401 cccagcggcc ctggctatcg gtcataaact gtcctctatt cggccgcgat cagctggccg
     2461 cgcggcgagc tggcggattc gaccttctgg aaatcgacca cctgctgcca ggcggcgaac
     2521 acttcggtgg gcgtgatgac gaccagctcg cccggattgg cggcggtcag cgccgccgcc
     2581 gtcgccgctt cctcgccggc gatgaggtgg atcgaggctt ccgatgcgcc gccctggagc
     2641 gcgccttcct gcaaaaggtt catgatctgg ccgcgcggcc ggccgcgggt cgaagggtcc
     2701 tcgcggaaga aaagctcgtc gaagatgccg ccggcgatgc ggccgagctc caatatgtcc
     2761 ccgtcgcgcc ggtcgccggg gatggagatg caaccgatcg cccttttgta gcggtggcgc
     2821 agcccgcgca cgacctcgcc cagggcggtg aggccggcgg cgttgtgcgc ataatcgacg
     2881 atcacccgaa agccgagcgc gtcatggacg ttgagccgcc cgggattctg ctcgaagctc
     2941 gactggaagc ccatcaaagc ggtgcggatc gtcaggatcg ggatcttatg cgcgacgcac
     3001 attgccaccg tcgccagcgc gttggcgatg ttgaacgtcg ccatgccgtg cagcgtcgcg
     3061 ggtatgtcgc ccgccttcat cacgaagtcg cggcggccgt cctggtgaag cacgatcgtg
     3121 ccgccctcct cgcccggctc gcgcaccacg gcggtgccgc ctccgtcgag atgccggcgc
     3181 agcaacggcg gcatctcgtc atcgccgtgc agcgagaacc agatgatctg cccgccggcg
     3241 cgccgcgcga tccgcacgca ttgcggatcg tcggcgttca gcaccgaagc gccgccgcgc
     3301 cggaccgtct cgacgatcag cgacttgacg ttggcgaggt cgtcgagcgt ctcgatcccc
     3361 ttgagcccga gatggtcggc ggtgacgttg agcacgaccc cgatatcggc ctcgtcgaac
     3421 gccagccctt cgcgcagcag cccgccgcgg gccgtctcca gcaccgcgac ctcgaccgtc
     3481 gggtcgcgca gcaccagccg cgcgctgcgc gggccggtgg cgtcgccttc ggagaccagg
     3541 aggccgttga cgtagacgcc gctggtcgaa gtgaggccga ccgtgcagcc ggtatagcgc
     3601 agcacctgct tgagcatccg cccgaccgtc gacttgccgt tggtgccggt gatggcgaag
     3661 atcggcaccc ggctgctccg gccgcgcggg aacagcatct ccatcaccgg cttagcgacg
     3721 tcgcgcggcg ccccttccga tgggtcgaga tgcatccgga aaccgggcgc cgcattaacc
     3781 tcgacgatgc cgccgccggt ctcgcgcacg gaccgggtga tatcgggcgc gatgaaatcg
     3841 ataccggcaa tgtcgagccc gaccaccatc gccgcgcggc gggcgatctc ggcattgtcg
     3901 ggatggattt cgttggtgcg gtcgaccgcg gtgccgccgc tcgacagatt ggcggtggcg
     3961 cgcagcatca cgatgtcgcc ggccgccggg atgctgtcga ccgtgaggcc cgagcggccg
     4021 atatattcct cgacatgggc atcgatccgg atccgggtca tgacattctc atgcccgtcg
     4081 ccgcgccgcg gatcctggtt cacggtcgcg atcagctcgg tgatcgtatg aaccccatcg
     4141 ccgatcacat gcgcgggaat ccgctcggcc accgcgatca gcttgccgtc caccaccagg
     4201 atccggtggt cgtggccgtg gaaatattgc tcgacgatga tgtcgcgacc cttggcctgc
     4261 gcctgcgcct cggcgaagcc gaaacggagc tgctcctcgt ccatgatgcc gatggtgacg
     4321 ccgcggccgt gattgccgtc gagcggcttg gtgaccaaag ggtagcgcag ccgcttcgcc
     4381 tcgcgcaccg catcctcgac atttcgcacc acgaccccgc gcggcaccgg gatgccgctc
     4441 tcggcaagca gcttcttggt gaggtccttg tcgccggcga tctcgaccgc gaccagcgac
     4501 gtccgcccgg tgatgctggc gcggatgcgt tgctggtggc ggccgtggcc gagctggatc
     4561 aggctctgct cgttgaggcg catcaccggt atgcggcgcc gcgccgcctc gtcgatcagc
     4621 gcctgggtgg tggggccgta gctggtgcgg cggacgagcc gcttgagctc cgccagccgc
     4681 cgcccgaagt cgaactcgcc ctggaagtcg tagacgcgct cgagccgctc gacgcccttg
     4741 aggtcgtcgg gcagcagcga gttgaccagt tcgagcgcga tccggcccgc catcagcccg
     4801 acccgctcgt cgcgataggc gtacatgatg ttgtagacgc cgggcttgcc cttcaccgac
     4861 cgcgtcttgc cgcgggtgac acggttccca gccatggtct gcagctcgat cgcgacatgc
     4921 tcggcgacat ggccgatcca ggttccctcg cgcagcctgc ggatgaagcc gccgcgccgc
     4981 ttgaagcagc agccgtggcg gtcgagcccc gggagcatct cgaccagccg gtcggcgaac
     5041 cccgggatgc ggtcggacgg ccaatcctcc agccgcccga gatcgaccat gatccggacg
     5101 aacggcgtgt ggctgtaata atgaggacca cgatagacac cggtctcgag caccttcatc
     5161 ggctcggtca gaagccgggt cgcggcatcc gaccgcgtgc cgaagaataa agcgcctcgt
     5221 tccattctcg cctctggtgg cgcagtcccc ggagcagcgc gaccgcgccg gaacaggcgc
     5281 cggggaagcg cgggttggaa tggatgttaa atgcccgaaa cgacagccga gttccggcgg
     5341 aacatgttga tccaagtcaa tccctgcacc cgattcgagc cgctccgggg gcgccgtccg
     5401 aaggctattt cagccgctcg atccgctgcg cggcatcgtc gagtatggcg gcgatttcgt
     5461 gcagcctttc ctcgtccgcc ccctcctcgg tcacccggtt ccagagcgct gcgagcaggt
     5521 tgcgcaccgc ccggccggcc gggccgccgc cggccttgcg acggtcctcg cccatgccgg
     5581 taagccgcgc catcagcctc gccagctcct cggcgcgctc cgcgagatgc gcgcgcccgt
     5641 cggcggtggc ggcgaacgcc ttgcgcgcgc cctccgaagc ggcttcctcg atcaggccca
     5701 tgtcctggag catggtcagg gtcggataga tcacgcccgg gcttggcgca tagctgccgt
     5761 gggtcagctc ctcgatcgcg cggatgaggt cgtagccgtg gcgcggctcg tcggcgatca
     5821 gcttcaggag caccagcctc agctcgccgg catcgaagcg gcggcggcgg cgcgagtcgc
     5881 ggccccagcc gccggcttcc gcgccccatt cgtcctggcc ccagccgcgc ccccagccgc
     5941 cgcggcccat gccggccgcg aggatggccg ccggaatgtc gaagccgcgc ccgccgcagc
     6001 catgcccgaa ccgaacccgc atgatcttcc tcctccttaa gttatatctt agatactctg
     6061 cagcagactt agtgtcaagg gtgtattgca tgaacaacac agccaacacg ctttttccag
     6121 tctgccccgg ctgccctaga tgccgcgtcg taaagcagcg ccctgcggca gcagccgcga
     6181 agcagaaggc gagccgtgcg caatggcggg gatagagggc ggaaataatt tcttgtcagc
     6241 gacttagggc tgctcctcgg gtcatctcgc gccagccttc accacggcct cggttcaggc
     6301 ctcttatatt gattggacgc cgtgcttttc cggccttaag caacgggatg ggacgcgatt
     6361 cgcttcgggg gaggcggcgt tccggggcgg accagccggc tttcgatttc tcctggatac
     6421 gccgctgcgg tgctcgcagc atggcggttt tgcgcgtcgc cggtccggag atcggcgcgg
     6481 ccggccgcga cacgaggagc ccgtcatggc cgattacgtc attccgtcgc acaccgtttc
     6541 gggcgaccgc atcaccatgg gcggggcgga tacgctcctc gtcgagcagg gcgcatcgct
     6601 ctccgtgacc gcgaacgcgc aggcggtgcg cttcaccact cccaccgacg gggcggtgat
     6661 caccaatttc ggcaccatcg aggcgcttgg ggacgccggc gatccgcgcg cgatccgatt
     6721 cgagagcggg atcggcgaca ctctcaacgc gaccatcacc aacgcctcgt ccggctccat
     6781 ctacgcgcgc agcgacgcca tccagatcca agccggcacg ctcgccgccg gactgctgac
     6841 gatcgacaat gccggttcta tccgctcgct ccaaagccag gcgctcgacc ttgccaactc
     6901 gaccggcacc ttcaccgtcg agctcgacaa ttcgggaacg atctcggcgg aggccgacga
     6961 cgcgatccgc ttcggctcga tcggcaacgt caccaacgaa ggcaggatcg acggcggcag
     7021 cgtggacacc tattcgggcg ccgacggcct ccaatatgag gtgaacgcga ccgggacggt
     7081 caccaacaat tcaggcggca ccatcatcgg cgaccgccac ggcatcaatg ccggcatcgg
     7141 ctcggtcatc accgtcctga actcggtcgg aggcacgctc gaggggcgca acgggtcggg
     7201 cgtcggctcg gacggaaccg cgaccatcac caattacggc tccatcatcg gctccttctc
     7261 caattcggtc gggtcggaca agaacggcac caccgtcggc gagctcgacg gcggcgggcc
     7321 cgacggcatc aatgacggcg acggcgacgg catcgacgtc gacggcaagg cgaccatcct
     7381 caacggcggc ctgatccacg gcgtctcggc cggcggttcg gggtcggacg gactgttcaa
     7441 caccgccgag ggcatcgccg ccggcggcgg atcgatcgac aatctcgcgg ccggcatcat
     7501 ccgcggcggc ggcctcggca tcctcatcga cgattcctcg cagggcaacg cgttcttcct
     7561 gacgacggtc gacaataagg gcctgatcga gggcctcacc ggaaccggca tccgcatcgt
     7621 cagcctgctc gccgacgtca tcgtcaacga cggcaccatc aagggcggca acggcttcgc
     7681 catccagttc ggcgccggcg acaaccggct ggtgatcaag gacggctcgg ccatgaccgg
     7741 catcgccgac ggccagggcg gcgtcaacac gctcgattac cgggtgttcg gaaccggcgg
     7801 aaccgtcgtc aagctcgcta ccggggccgc gaccggcacc ggcggcatcg acaatttcgt
     7861 caacctgctc ggctcggccg gggtggacaa tttcgccgga agcgcggccg ccgagctgct
     7921 cgacggcaac aacggcgccg acgtgctgag cggcggcgcc ggcaacgacc ggctgttcgg
     7981 ggacgaaggc aacgacctcc tcaacggcgg cgcggacctc gacgcgatgg cgggcggcct
     8041 cggcaatgac ggctattccg tcgacaatgc cggcgaccgg gtcaccgagg cattcgacgg
     8101 cggcatcgac cgcgtgtcga gctcgatcaa ccacattctc gccgccaacg tcgagaattt
     8161 gacgttggtc ggcactgccg ggtccggcag cggcaacatc ctcgccaaca ccattaccgg
     8221 caatgccggg gacaacaagc tcgatggcaa gggcggcgcg gaccggctcg tgggcggcgc
     8281 gggaaatgac ggctacgtgg tcgattccag cggcgaccgg gtcatcgagg ccgtcgccgg
     8341 tggcaccgac cgcgtgtcga gctcggtcac ccacaggctc gccgccaatg tcgagaatct
     8401 ggcgctgacc ggcaacgagg cgatcgatgg cgccggcaat ggcgaccgca acggcatcac
     8461 cggcaacgcc gccaacaatc ggatctcggg cggcggcggc aaggacgtgc tgaccggcgg
     8521 cgccggcgcc gaccagttcc ggttcgacac cgcgctcgat ccggtcaaca attgggacga
     8581 catcctcgat ttctcggtga cggacgacgt gatcgtgctc gaccggacga tcttcaccgg
     8641 catcggcacc aatggtaccc tcgcctcctc ggcttttgtc gccgggacgt ccgcgctcga
     8701 cgccagcgac cgcatcgtct tcgacaccgc caccggccgc atcttctacg acgtcgatgg
     8761 cttcggcggc gcggcccagg tgctgatcgc ccgcgtcgat atcaacacgc cgctcaccca
     8821 cctcgacttc ttcgccgtcg cctgagcggc cggggctaaa cgttctcttc gtcatgccgg
     8881 acttgatccg gcatctccct tcttcttctc cgagccgatc gtgagttgaa gaagggagag
     8941 ccggatcaag tccggggtga cgatgaacct tgagcgggcg ctgagccccg ctcccgcgcc
     9001 gctttccgga acagccaacc cgatccgagc cagatcgctg cgaagcaccc gttgagcgcg
     9061 gccagctcca acgccgcccc ggccggcagc ggcccgagtc cggcggcgat cgcgatcggt
     9121 gccgccacca tctgggcgag cgccgtcgcg gccatcgccc gcgccattcc ctcagccctg
     9181 aagcgcgcgg caagcgcgcc gaccagcgcc accccgagta ccccgccgta aacgaggttg
     9241 gcgggattat cctcgctccc gatcacaccg acggccaggt tgatccagac gagcgtgaac
     9301 gccgccgcca gcgcgaggcc ggcgcccgcc cgatacgccc agttgcccga catcctcgcc
     9361 gccagctcat agccgccgcc ggccgcggcc agcatcgtgc ctaacagcac gaaatcggcc
     9421 gcatcccact ggacctcctt gctgacctgc atcgccagca gcagcagcag cagcattccc
     9481 gccgcgccgc cccaggccgc caccctccag ccgctcccgc cggccgcgtg ccccatcttt
     9541 cccgccataa ccccgcctcc tgatcgactg agaaatcgaa catcccatat gccacggcgc
     9601 cttgcaccct tgatgccggc gtgaaggaga aatggtgcag aaacggcaag ccgtagcgga
     9661 ctgctcccag ccgccggccc ggcaggcttc ttgcggaacg gttcgcgcta gacgatcagc
     9721 cgccgccccg cagcggcacg acgcgctcca tgtccccgcg cggcatccac ttggcgccga
     9781 gcgcctgcgc tcgttgccgg ggcaggccga gttgaagcag cgccgtctcg agcgcctttt
     9841 gggccggcac ttcgacatgc ggtttgacgc cgatcccctc ccagccgacg ccggtctgcg
     9901 ggtcgaacgg ccggccgatc gggatgaacg cagaaaagcc ctccggcaat tcgaccaccc
     9961 ggccgaaatg tcccatcccg gcggtggtct cgccgatgat cgtggcccgt cccgtccgct
    10021 tcagcgcgag cgtcaaatgc tcggccgccg agccggtgta accggaggtg agaacgaaaa
    10081 gcttggcgcc gcgaaggccg gtcggcgccg cgttcggcgc ggcaaggtgg acttgcctga
    10141 cgaatcccgg cggcccggcc aggcgcttca gcgtcgcggt atccggcctg gcacggaatg
    10201 ccgcctcgcg cgtgtcctcg tagatgagct cggctggtcg gtcgtaaagg tatgaggcga
    10261 ggaggtcgaa ctcgtccgta tagccgcccc catgggcgcg cacgtcgatg ataagcacgc
    10321 gcgccgaagc gtgtgtctcc aggaactccc gaaccgcggc cagcgacgcc ttggaggccg
    10381 ggaacagttc gaagccgata taggcgacgc cgtcggccag ccaccccgat ttgtcgattc
    10441 cggtttcggc gctttgctgc tcggctgccg acgccgaagg cgcgccgagc atcggctggc
    10501 gcgacggcgg gctgagccgc agatgcccgt cggcccgtat cttctggaga tcggccgtga
    10561 caatttgagc caacttgtcc ggcgccagcc cgcgatagcg gccggagacc agccgctgcc
    10621 gcagctcgcg cgcataagcc tggccggccg agggatcgag gaaatatcgc tcgagcgagc
    10681 gggccatctc ctcgaccacc ttgtgcagcg actgttccgc gagcggcggt tccgcctgaa
    10741 cgttctgcac ccaaattccc gcggaagccg caatcgcaat ccccgccgcg accaggaggc
    10801 gcgtccgcac gtagcgcagt cggaaagtcg tcatcattcg cccgccatcc ttgccgccac
    10861 ggcgccctcg ccgcgccgcc tccctgaaat catatacagg gcgggcgcgc gcggaacgct
    10921 tcctcgcggg ccgctacgcg aaaatgttgc aagctttgcg ccttccatgc attctctgcc
    10981 catggacgca ttggacaggg cgctgctgac gatacttcag gcggatagcc gcctcacctc
    11041 cgagcagatg agcgagcggc ttcacctgtc gccttcggcg gtcaaccggc gggtgcagcg
    11101 cctgtggcgg gaggggatca tcgcggccga ggtcgtcgtg ctcggcgaga agctgcgctc
    11161 tcgcctcacc agcgcgatcg tcaacgttca gctcgaccgc catcatccct cgcaagccga
    11221 cgagttgcgc cgcatcctca gggcatctcc gcacgtccag ctatgtctgg aaatcaccgg
    11281 aacctccgac gtgctcctcg tcgtctcggt tcgcgacatg gctcacttca acgccttcac
    11341 cgacgcgctc gcccagatgc cgctggtgcg gcgctacgaa acgcatttcg tcaaacgctc
    11401 gatcaagttc acgaccgcca tctcgctgga ggaagactga agccggcgcg ggggcaacaa
    11461 ccgctggttg gacttggtgt cgctgaacct gaccaccgcc ttgactgcgg tgccgttcgg
    11521 gagcttggat cggccgccgc gcgaaggcaa tttggccgca agtgtgatcc ggggaagaag
    11581 cgcgtgccgc ggttgatcct gttcaacaag ccttacgaag tgctgtccca gttcaccgac
    11641 agcggtggcg gggcggcgcg gtcgaccttg tcggacttcg tccgcttgcc ggccgtctat
    11701 ccagccgggc ggctggaccg cgacagcgag gggctgctgc ttctgaccga cgacggcagg
    11761 ctgcaggcgc ggatcgccga tccgaagttc aagctgccca agacctacct cgtccaggtc
    11821 gagggcgacg cccagcccga cagcctggaa cggctgaagc gcggcgtcca actgaaggac
    11881 ggcatgaccc gcccggccga ggccgagcgg atttccgagc ccgctttgtg gccgcgcgac
    11941 ccgccggtgc gcttccgccg gagcgtgccc gattgctggc tgaagctgac gatccgcgag
    12001 ggccgcaacc gccaggtgcg ccggatgacc gccgccgtcg gccatccgac gctgaggctg
    12061 gtgcggtgga gcgtcggcgg ctggacgatc gaaggcctgg cacccggcga gtggcgcgag
    12121 gtgcccgtcc attaaggccc tgccccttca gtccgcgtgg ccaaactcga cgctgccctg
    12181 gttcaaaagc tccgccacca gcgtcaccac cgcgtccgat cccgcgaggg caggatcgac
    12241 gtgggcgcag tcgcttccgc aaagctcctc ggcgacccgg gccgtctccc cgtcgcagtc
    12301 caaactggcg ccgtccgcga acaggagcac ggatccatcc tcctgccgaa ggaacgagaa
    12361 gcggctgccg gggctgcggc gcagaggcag gcttcgcgcg atcaaccggc gcacctcatc
    12421 gacctcgatc ggctcttccc gcgcccggtc catctcggga tgcttgggcg aactattgta
    12481 gcggccgaac cagcggccga aggcggccgc gtcgagcatc ctggcggtcg ccatggcatg
    12541 cagacgcgcg atggcttccg cactgatctc gcccggattc cgctgctcct cgagatccgg
    12601 gtcggcgtag cgcggatcgt cccgcagcgg ctcgagcaga tgctcgctcc acccggcgag
    12661 aagctcggac gacgagggcg cgcgaaaccc gatcgaatag gtcatgcagt cgtggccgac
    12721 ggcgatgccc tcatgcgcca cccccggcgg cacatagaga atgtcgccgg gccccagcac
    12781 ccaatcgtca ctggcttcga agccggggag caggcggaga tcgtcgtgcg gccgaagcgc
    12841 ggtgctggcg tcgcaatccg ggccgatccg ccagcggcgc ctgccgaggc cctggaccag
    12901 gaacacgtca tatttgtcga aatgcgggcc gacgccgccg ccgtcggtgg cgtagctcac
    12961 catgatgtcg tcgatgcgcc agttcggcag gaagcggaac ggcggcagga gcgcggccac
    13021 ttccggcacg agccggtcga cagactggac cagcaacgtc catggccggt cgcccagttg
    13081 ctcgaagcgc cgttcgggaa acggtccatg ctcgagtttc cacgtattgc caatggtcag
    13141 gatcaggcgg gactcgactt cctcttcgca ggcgaggccg gccagctcgt ccggctcgag
    13201 cgggttggcc caagccgtcc acggattgcg gatcagcaac ggcttcttct gccaatgctc
    13261 ggagaggaaa tggcgcgcgt cgaactgttt cagctgcatc cgatcccgcc gtcgctatcg
    13321 atcttcccac gtcccatagg ggaccgccgc ctccacggat atcccccggc cgagccccga
    13381 tattgtagct gcttccgccg cttgtgcgtg gtacaatgta tcattcacct gtcgggagta
    13441 tccccatgcg tcagttcgtc gcgttcgtat cgcttgccgc tcttgccggc tgccaatcac
    13501 cgcctcaacc gcgcggcgtg gagccggacg agacgctgct cagcgtcgcc gcctccggca
    13561 gggtcgatac gcgaccggac gaggcgcggc tccagctcgg agttgtctcg gtggcggcga
    13621 cgtcgggcga tgcagcccgc gccaaccgcg agaagatgga ccgcgtcgcc gcgaccctcg
    13681 cccggctcgg cgtcaagccg gacgatctgc agacccgcaa cctcagcctg cagcgtatcg
    13741 aatatggcaa ggaccgcggc ggctatcgcg ccgaaaacgt ggtcgaggtg aagctgcacg
    13801 acatgacgaa ggtcggcgat gccgtcaccg ccgtcaccga agccggcgcc aacgtcatgt
    13861 cggggcccga cctcagggtc tcggatcccg aagcggcgtc gcgatcggcc tatgccgcgg
    13921 cttacaagtc ggcccggtcg cgcgccgagg cctatgcggg agcggccggg ctgaaggtcg
    13981 cccgggtcct ggcgatctac gatggcggcg aaggcgcgat gcctccggcc tatgggcgcg
    14041 gggttgcgat ggacgccacc gcgcaaagcg tcgcgccgcc accggtatcg gcgccgttca
    14101 gcgccggggt gaacacgtcc gaggttcgcg tccgggtcga tttcgcgctg agcgactaat
    14161 tggcgcggga cgaagacgat tccgagcatg acacggtgaa tccgaattcg ggagccggcg
    14221 gcgccttggg cgggagcagg aattgcagcg cctgcggagt gagctgctgg tagatggtcg
    14281 agtggaggag gtccggccgc gacgcgaggc accagggtga gcccgcgtcc cgaatcgcgc
    14341 cggtcacccg cttcatcgcc gccggctttt ccgcctgctc ccgggccatg gcgaggtaga
    14401 ccgggcggcc ttgctgcggg gcgtcgatct tcgccgcctc ggcgcgcgac agcttctccc
    14461 cgtcccacca caggctcgga tcgatcgcgg cataggcgtc gaacagcgcc ggttcgtcga
    14521 aataggtttc gaggatgaac aggcccgcca gcgattcccc gatcaccgcg tcgcggccgc
    14581 tggtccggta gctggcgcgg atcagcggct tcacttcgtc ccggatgaag gcccggaacg
    14641 cggccgaagc gccggccgtg ggatagcgtt tcagcaattc ggcatcggtc gtggggccgg
    14701 tcagctcctt gcgccggtcc ctggtctcga tcccgacgac gatggcttcg cccgagcggc
    14761 cccagaccgc gccgaggtgc agcacaccgg cgacgtgcag cagatcctgc tcgatcccgc
    14821 cgtcgatcag atagaggacc ggatagtgct tgtccggcgc cttcgcatag ccggccggca
    14881 ggaccacatt gacgacccgc gcctcgccca gcggcgccga cacgatgcgg tgggtgtcgc
    14941 caatgctgag cggcggcgcg ccgggaggca ccggcggcac cgcaaaggcc gcgctgcctg
    15001 ccgcaagcgc gagtgcgaag agccaaagtc tcatctgatc tccccggcag gccggcacag
    15061 gcgccgcacg gctgctaacc ccctgccccc gttcattgca agctcatggc cgcggcgcta
    15121 tctagaccgc cgatggcccg actcctttcc tcgctgctga tcgcgctcgg cttgctgctg
    15181 gccccgctcg gcatggcgaa cggcgccgcc gccgccatgc cgcaccacgg tgccgccatg
    15241 aatatggcag cagccgacgg cccctgcgcg ggcatgccgg ccccggccga cgagaagcgc
    15301 aaagccgatt tgaagctgag ctgcgccagc gcctgcgccg ccttcctccc ggccgtcgcg
    15361 ccgctcgctg ctcaagccgg cctcgcgccc gatcccgccg ccccgcttct gccccgccgc
    15421 ctcgccggcc tcgaccccga acgcgaaacg ccgcctcccc gaaccgctcc cgaaatttga
    15481 tccttcaaca gatttccagg agttacccaa catgaagatg ctgctcaccg tggtcgcgat
    15541 gatgatcgct tcgcccgctc tcgcccaggc cgccccggcc gacgcccacg ccgcccatgg
    15601 cggccacgcc gctcatgccg ccagcgccca tgccgggcac ggcgcccagg accatggcca
    15661 gaaggacgat ccgcacgcca agcatcaggg cgcgtgctgc aagcccgacg cggccggcaa
    15721 catgcccggc tgctgcgagg aaaaggacgg caagaccgcc gattgctgcc gcgagagcgc
    15781 cgacaagaag gacggccagg ccggtcacgg tcagggccgc cactaagcgg tacacggggg
    15841 acgcgcacct tcccgcggga aagtacgcgt cctcctccac aggattggcg acatgaacca
    15901 gcctatcggg cgccgcgatt tgctgcgcgc cggcgcgctc ggcgcggccg gcctcggcct
    15961 cgcgcgcctc taccccgcct gggcccagac cggcacgccg ggcctcgtcc cgcagcttcc
    16021 gaccctttcg ggcgaggaga tcgcgcttcg catcggccac acgccgttcg cggtcggcgg
    16081 ccgcactggc cacgccatca ccatcaacgg cacattgccc ggccctttgc tgcggctgcg
    16141 cgagggccag cggcttcggc tcagggtgga caaccagctc gacgaggata cgtcgatcca
    16201 ctggcacggc ctgctcgtcc ccttcgagat ggacggcgtc cccggcatca gcttcccagg
    16261 catcccggcg cgcggcagct tcacctacga attcccgatc gtccagtcgg gcacctattg
    16321 gtatcacagc cattccgggc tgcaggagca gatgggccat tacggcccga tgatcatcga
    16381 tccgaagggc gccgacccga tcgccagcga ccgcgagcat gtcatcgtgc tctcggactg
    16441 gagcttcttc cacccgcacc ggatcatctc gaagctcaag caggaaggcg gcttcttcaa
    16501 ccggcagaag gagactttgc tgggcaagcg cagcggcatg agcgcggagg accggggcat
    16561 gttcgcgcgg atgcggatgg acccgaccga catcgccgac gtcaccgagg ccgcctacac
    16621 cttcctcgtc aacggccacg gcccgggcga gaattggacc ggcctgttcc gtccgggcga
    16681 gcgcgtgcgg ctgcgcatcg tcaacgccgc cgcccagacc atcttcaacg tccgcatccc
    16741 cggcctcgcc atgaccgtcg tcgccgccga cggcaatccg gtgcgcccgg tccaggtcga
    16801 ggagttccag atcggcaatg ccgaaaccta cgacgtcatc gtccgcccgg cggaggatcg
    16861 agcctatagt ttcgtagccg aaacgatcga ccgctccggc atggcccgcg ccaccctcgc
    16921 cccccgcctc ggccttaccg cccccgtccc gccgctgcgc cggcgcccga ccctgaccat
    16981 gaaagacatg ggcatgggcg gcatggacca tgcggccatg ggccatggcg cgggtcacgg
    17041 ggcggcggcg cccgccatgg accattcgaa catggacatg cgcgacaaga ccaaggtctc
    17101 cttcccggtc ggcgtcggcg tcgacatgat cgcccccaat ccggtcgacc gcaccggcga
    17161 tcccggcatc ggcctcgaga ctgtcggcca taaggtcctc acctatcgcg atctcgtctc
    17221 gctccatccc aaccccgaca cgcggccgcc gacgcgggcg atcgagatcc acctcaccgg
    17281 caatatggag cgcttcatgt ggtcgatcga tggcgagcgg ctgagcgaga atcccgagcc
    17341 gatccgcttc gagcgcggcg agcgggtgcg ggtgacgatg gtcaacgaca ccatgatgac
    17401 ccacccgatg cacattcacg gccatttttt cgaagtggtg aacggccatg ccggcaacca
    17461 tccggtcaag cacacgatga tggttctgcc cggcggcaag gccagcttcg acatgaccgc
    17521 cgacgccccc ggggactggg ccttccactg ccacctcctc taccacatgc acgccggcat
    17581 gatgcgcgtc gtcaaggtcc gcccgctggc cggagccgcc gcatgaagca cctcttcttg
    17641 atcaccacct tcgccgccac ctttgccgcg cccgcgctcg cgcagcatgc cggccacgct
    17701 cctcctccgg ccgctcccgc cgacccccat gccgggcaca acatgccggc cgcacagccc
    17761 gctcccccgg ccgacccaca cgccggccac aacatgccgg ccgccgaacc cgcagccccg
    17821 gccgatcctc atgccgatca caccatgccg gttgccgagc cagccgcccc ggctgaccct
    17881 cacgccgggc acgccatgcc cgccgcccct gccgatcccc atgccgccgc cgcccccgcg
    17941 ccggacgccg gccctcccgt cgccccgtcc ccgccggccg cgctcagcgg gcccgaggat
    18001 gccgccgacc tcgtctacgg tcgcgacgcc atggccgccg cccgcgccgg catgcgcgag
    18061 gagcatggcg gcatgaagac ctacaaggtc ctcttcgacc agctcgaggc gaaactccgc
    18121 gacggccgcg acggctattc ctgggacgcc caggcctggt atggcggcga catcgacaag
    18181 ctttgggtca agaccgaagg cgaaggggcg ttcggcgaag cggtcgagcg ggccgaagtc
    18241 caggcg
//
LOCUS       DASVRK010000037         6535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_503621, whole genome shotgun sequence.
ACCESSION   DASVRK010000037 DASVRK010000000
VERSION     DASVRK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503621"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1640
                     /locus_tag="VF589_01350"
     CDS             117..1640
                     /locus_tag="VF589_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256251.1"
                     /translation="MSFLRTFLLASTVIPLAACGGADDVASPGEGVIVVPTPTPSPTP
                     TPTPTPTPTPGEGPAADCPAGTDNVGVINNQRNCQISGTITGARTIRNLPGVIYSLNG
                     QVQVGRDLGGNGQAPGGAQGILTVEAGVVMFGSSGADFLLVNRGSQIFAEGTETKPII
                     FTSRQNVEGTAEGSSIGLWGGVVILGRAPTANCLGEGVEPGSANCQGAVEGTSGDNFF
                     GGASPSDNSGRLRYVQIRYPGFEVSEGNELNGLTLAGVGRGTTIDHIQIHNSSDDGIE
                     WFGGRVNQKYVVVTGADDDSLDIDTGYKGALQFAIVRQRNSAGDHLVEGESSGDENRA
                     PRTNIQIANATMVSGGESSAILLRGGMDFSLLNSVVARAPTCIDIDGNGTAQAAGAGP
                     DEEGPPRFESVYLSCTAPFEDDTDVTAQQVAQIFNSGSNNVANGESTLQNGFINGPNE
                     SARPAFRASTVNPFFEDVNYIGAVRDAADTWWHNWTCGLGANSMSCTQAPANTATAA"
     gene            1714..4458
                     /locus_tag="VF589_01355"
     CDS             1714..4458
                     /locus_tag="VF589_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8256252.1"
                     /translation="MTNFATFGSLLLLSTALVAPAALAQTPTTSEAASPPAGTGAAAD
                     PAADPAEAEAPAEEQEIEISAPGADNDDQAIVVVGTKIPNSIRRTPEVISVLSSEDIA
                     RTGEGDIAGALQRVTGLSVVGGRFVYVRGLGERYSLALLNGSPLPSPEPLRRVVPLDI
                     FPTDVIASSVVQKSYSVNYPGEFGGGVINLTTRSMPNEGFLTIGASIGIDTETTNELG
                     YTYYGSDTDWTGFDNGTRDSPKGFKEALDSGNPIIIGPNFTEEQVKGFTSSLVNAPTT
                     LIQRNRHMPPNWAVNLSAGNTWNMGEFEFGVIAAAGYSNTWQTKSGLQQLSGGVAVGD
                     DGGEILRPDQDYRFLSTEDRIVVNALLGLGLEWGEHQLRWTNLYIRDTIKEARIQAGT
                     DEINVGDEPIQIGNTAWFERQLIDTQFVGEFDWDPISLDIRATYANSQRESPYERSIG
                     YRYNETAGDYVNNLTGPGQFARIAFSDLSDDVWSGAADLAYELPTTRPITVSAGAYYY
                     KNKRAAERRAFRYTALSPLPFAVAQERPDYLLSDFNVEAYDIVLSDVSSAAGVAAYEA
                     DLEVVAGYAQAEGELAESLRLQAGVRYEDGRQRVNPIDLFGIGGNSVVPTEINENYLL
                     PAATLTWILAEDMQLRFHASKTIARPQFRELAPQQYLDIETDRTFFGNQFLTDSKLIN
                     AEARYEWYFGRDQRLSLAGFYKKIDSPIESVAFEQGGTFFTTFANAPEATLVGLEIEA
                     QKYFPLDGLGGGFFAPRRLVLIGNYTYTDSKIKVAEGDTTIPVGSGGVAVPASNLFTD
                     GERLTGQSSHLANLQIGLENTDRLSQQTLLLSYAGRRVTNRGPSGQPDFIEEPGLRLD
                     FVMREGVEIMGKAVELKFEARNLLGENYEEYQTLNASRIDLNTYDLGRTFSVGLGVTF
                     "
     gene            complement(4606..5223)
                     /locus_tag="VF589_01360"
     CDS             complement(4606..5223)
                     /locus_tag="VF589_01360"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8256253.1"
                     /translation="METAALIDRSSGVQKVPSPKLDLFIKRGFLSPDECASIIRLIDS
                     RRRPSTLSDDLGDTLFRTSETCDLDRGLPEVAALDRRISDFAGIDPRFGEPIQGQRYA
                     EGQEFKAHTDYFEPNGPDFIRFCSVAGQRTWTVMIYLNEPAAGGATRFKTINKTVQPE
                     TGKLLAWNNIGPDGRPNVNTLHHGMKVRSGTKYIITKWFREKPWR"
     gene            5427..6299
                     /locus_tag="VF589_01365"
     CDS             5427..6299
                     /locus_tag="VF589_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX8256254.1"
                     /translation="MRLALDPRARRLLRRVPRTTVYTALELALLSLLAVQAARLVWTI
                     VTPVDPVGNWRAGSSLQAPPQTSTALLGEFDPFFRLSGEAAPVVVTSLNIKLFGIRED
                     RASGRGSAIISTPDGTQRSFAVGEEIIPGVTLTSVAFDNVTITRNGETEQLFLDQSPS
                     ADVVGPDSGAATPPPPTGLLLPASPPSSPVAPPPAPSPSPGGSLASEARFQPRMNGSQ
                     ITGITVQPQGSGNALRAAGLQAGDVILSVNGRRITSPDQARSMANQFRGEREVTVQVE
                     RDGRVMPVRVRPGQ"
     gene            6296..>6535
                     /locus_tag="VF589_01370"
     CDS             6296..>6535
                     /locus_tag="VF589_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HEX8256255.1"
                     /translation="MRRGLGIALALVLAAPAVTPAWSQYVLNLREADIRAFIEDAARV
                     TGRTFVIDPTVQGKVSVVTQRPLSRTEYFEVFLSTL"
BASE COUNT         1122 a         2280 c         2093 g         1040 t
ORIGIN      
        1 ttcaaacatc gtcctcatgt aatattacta tcacagaatc agcctagctc cccccatcag
       61 aagaacggac tcgcacaggg cggctccgag tctcgtacga acaagggggt tccttcatgt
      121 ctttcctgcg cacgttcctg ctcgccagca ccgtcattcc gctcgccgcc tgcgggggcg
      181 cggacgacgt ggcctcgccc ggcgagggag tgatcgtcgt cccgaccccg acgccgtcgc
      241 cgactcccac cccgaccccc actccgacgc cgacccccgg cgagggcccc gccgccgatt
      301 gcccggcagg caccgacaat gtcggcgtca tcaacaatca gcgtaactgc cagatttcgg
      361 gcaccatcac cggcgcccgc acgatccgga acctgccggg cgtgatctat tcgctcaacg
      421 gccaggtcca ggtcggccgc gacctcggcg gcaacggcca ggctcccggc ggcgcgcaag
      481 gcatcctgac ggtcgaggcc ggcgtggtca tgttcggctc gtccggcgcg gacttcctgc
      541 tcgttaaccg cggttcgcag atcttcgcgg agggcaccga gaccaagccg atcatcttca
      601 ccagccgcca gaatgtcgaa ggcacggcgg aaggcagctc gatcggcctg tggggcggcg
      661 tcgtcattct cggccgcgcg ccgaccgcca actgcctcgg cgagggcgtc gaacccggct
      721 ccgccaactg ccagggcgcc gtcgaaggca cttcgggcga caatttcttc ggcggcgcct
      781 cgccgtcgga caatagcggc cggctgcgct acgtgcagat ccgctacccc ggcttcgaag
      841 tctcggaagg taacgagctt aacggcctca ccctcgccgg cgtcggccgc ggcaccacca
      901 tcgaccatat ccagatccac aacagctcgg acgacggcat cgaatggttc ggcggccggg
      961 tgaaccagaa atacgtcgtc gtgaccggcg ccgacgacga cagcctcgac atcgacaccg
     1021 gctataaggg cgcgctgcaa ttcgcgatcg tccggcagcg caactcggcg ggcgaccacc
     1081 tcgtcgaggg cgaaagctcg ggcgacgaaa atcgcgctcc gcgcaccaac atccagatcg
     1141 cgaatgcgac gatggtgagc ggcggcgaat cgagcgccat cctgctgcgc ggcggcatgg
     1201 acttcagcct gttgaactcg gtggtggcgc gggcgccgac ctgcatcgac atcgacggca
     1261 acggcaccgc ccaggcggcc ggcgccggcc cggacgagga agggccgccg cggttcgaat
     1321 cggtctatct gtcgtgcacg gctccgttcg aggacgatac cgacgtcacc gcccagcagg
     1381 tcgcgcagat cttcaacagc gggtccaaca atgtcgcgaa cggcgagtcg acgctgcaga
     1441 acgggttcat caacggcccg aacgaaagcg cccgccccgc cttccgggct tcgacggtta
     1501 acccgttctt cgaagacgtg aactatatcg gggcggtccg cgacgccgcc gatacgtggt
     1561 ggcacaattg gacctgcggg ctcggcgcga attcgatgtc gtgcacccag gccccggcga
     1621 acaccgccac cgcagcctga tcgacccaat gacactatgg gcggcggctt cggccgccgc
     1681 cccttgatgc acgtgattac cgggggctcc gccatgacca atttcgccac tttcgggagc
     1741 ctgctcctgc tatcgaccgc gctcgtcgcg ccggccgccc tcgcgcagac gccgaccacg
     1801 tccgaggccg ccagcccgcc cgccggcacc ggcgccgccg ccgatccggc cgccgatccc
     1861 gccgaggccg aagcgccggc cgaggagcag gagatcgaga tctcggcgcc cggagcggac
     1921 aatgacgacc aagcgatcgt cgtcgtcggc accaagatcc cgaacagcat ccgccgcacg
     1981 ccggaagtga tctcggtgct gtcgagcgaa gacatcgccc gcaccggcga aggcgacatc
     2041 gccggcgcac tccagcgcgt caccggcctc agcgtcgtcg gcggccgctt cgtctacgtc
     2101 cgcggcctcg gcgaacgcta ttcgctggcg ctgctcaacg gttcgccgct gccgtcgccc
     2161 gagccgctgc gccgggtggt gccgctcgac atcttcccga ccgacgtgat cgcgagctcg
     2221 gtcgtgcaga agagctattc ggtcaattat ccaggcgagt tcggcggcgg cgtcatcaat
     2281 ctcaccaccc gctcgatgcc caatgagggc ttcctgacga tcggcgccag catcggcatc
     2341 gacaccgaga ccaccaacga gctcggctat acctattatg gcagcgacac cgactggacc
     2401 ggcttcgaca acggcacccg cgatagtccc aagggcttca aggaagcgct cgacagcggc
     2461 aacccgatca tcatcggccc caatttcacc gaggagcagg tcaagggctt caccagcagc
     2521 ctggtgaacg cgccgaccac gctcatccag cgcaaccggc acatgccgcc caattgggcg
     2581 gtcaacctca gcgccggcaa cacctggaac atgggcgagt tcgaattcgg agtcatcgcc
     2641 gcggccggct acagcaacac ctggcagacc aaaagcgggc tccagcaatt gtcgggcggc
     2701 gtcgcggtcg gcgacgacgg cggcgagatc ctccgtcccg accaggatta ccgcttcctg
     2761 tcgaccgagg accggatcgt ggtcaatgca ttgctcggcc tcggcctgga atggggcgag
     2821 caccagctgc gctggaccaa tttgtacatc cgcgacacca tcaaggaagc gcgcatccag
     2881 gccggcaccg acgagatcaa cgtcggcgac gagccgatcc agatcggcaa caccgcttgg
     2941 ttcgagcggc agctgatcga cacccagttc gtcggcgagt tcgactggga cccgatcagc
     3001 ctcgacatcc gtgcgaccta cgccaactcg cagcgcgaat cgccttatga gcgctcgatc
     3061 ggctatcgct acaacgagac cgccggcgat tatgtgaaca acctcaccgg ccccggccag
     3121 ttcgcgcgca tcgccttcag cgacctcagc gacgacgtct ggtcgggcgc ggccgacctc
     3181 gcttacgagc tgccgacgac gcgtccgatc acggtctcgg ccggcgctta ttattacaag
     3241 aacaagcgcg ccgccgaacg ccgggcgttc cgctacaccg cgctctcgcc cttgccgttc
     3301 gcggtcgcgc aggagcggcc ggattacctc ctctccgact tcaacgtcga ggcgtacgac
     3361 atcgtcctca gcgacgtatc gagcgcggcc ggcgtggccg cctacgaggc cgatctcgag
     3421 gtggtggccg gctatgctca ggccgaaggc gagctggccg agagcctgcg cctccaggcg
     3481 ggtgtgcgtt atgaagacgg ccgccagcgg gtcaacccga tcgacctgtt cgggatcggc
     3541 ggcaacagcg tggtcccgac cgagatcaac gaaaattacc tgctgccggc ggcgaccctg
     3601 acctggatcc tggccgagga catgcagctc cggttccatg cctccaagac gatcgcgcgg
     3661 ccccagttcc gcgagctggc gccgcagcaa tatctcgaca tcgagaccga ccgcaccttc
     3721 ttcggcaacc agttcctgac cgacagcaag ctgatcaacg ccgaggcccg ctacgaatgg
     3781 tatttcggac gcgaccagcg gctgagcctg gccggtttct acaagaagat cgacagcccg
     3841 atcgagtccg tggcgttcga gcagggcggc acattcttca ccaccttcgc caacgctccc
     3901 gaggcgaccc tggtcgggct cgagatcgag gcgcagaaat atttcccgct ggacgggctt
     3961 ggcgggggtt tcttcgcgcc gcgccggctc gtgctgatcg gcaactacac ctacaccgat
     4021 tccaagatca aagtcgccga gggcgacacg accatcccgg tcggcagcgg cggggtggcg
     4081 gtgccggcgt ccaacctgtt caccgacggc gaacgcctga ctggccagtc gagccacctc
     4141 gccaatctgc agatcgggct cgaaaatacc gaccggctgt cgcagcagac cttgctgctc
     4201 agctatgccg ggcggcgcgt caccaatcgc ggcccctccg gtcagcccga cttcatcgag
     4261 gagccgggcc tgcggctcga cttcgtgatg cgcgaaggcg tcgagatcat gggcaaggcg
     4321 gtcgagctca agttcgaggc gcgcaacctg ctcggcgaga attatgagga atatcagacg
     4381 ctgaacgcca gccggattga cctaaatact tacgatcttg gccggacgtt cagcgtgggt
     4441 ctaggtgtga ccttctagtc agcttccggg cggcgctatt ccctagaggc gccgcccaac
     4501 cttttcaaac ccgacagaaa gcgttccccg gcgaaggccg gggcccagcc cgcgccgcag
     4561 gcgcgacggc cgaggcgggg catctcaaca ccgccccctc cgcactcagc gccacggctt
     4621 ctcccgaaac cacttggtga tgatatattt ggtgccgctg cgcaccttca tgccatggtg
     4681 caaggtgttg acgttcggcc gcccgtcggg gccgatattg ttccaggcaa gcaatttgcc
     4741 ggtctccggc tggaccgtct tgttgatggt cttgaaccgc gtcgcgccgc cggcggcggg
     4801 ttcgttgagg tagatcatca ccgtccaggt ccgctgcccg gcgaccgagc agaagcggat
     4861 gaaatcgggt ccgttcggct cgaaataatc ggtgtgcgcc ttgaactcct gcccttccgc
     4921 gtagcgctgg ccctggatcg gctcgccgaa gcgggggtcg atgcccgcga aatcggatat
     4981 ccggcggtcg agcgccgcca cctccggcaa gccgcggtcg aggtcgcacg tctcgctggt
     5041 gcggaacagc gtgtcgccga ggtcgtcgga cagcgtcgac gggcgccgcc gggagtcgat
     5101 gagccggatg atcgacgcgc attcgtccgg cgacaggaag ccgcgcttga tgaacaggtc
     5161 gagcttgggg gaggggacct tctggacgcc cgaggagcgg tcgatgagag cggctgtttc
     5221 catggcgcgg gtttagcgcc ccgccttcgc cattgcagcg gatttttgct tgcaccgccc
     5281 ggcgggagcg ggcagggctc aggccaacac cacggaaaca ggcgattttt cgggtgaatg
     5341 ggcgacgcgc cgtcagattc ctgtcacaaa tcttcaattg gcgtgtcatg gtgggtccct
     5401 agggactcgg tggcggggag taatcgatgc gtttggctct ggacccgcgg gcgcgccgct
     5461 tgttgcggcg ggtgccgcga acgacggtat atacggcgct tgaactggcg ctgctgtcgc
     5521 tgctcgcggt gcaggcggcg cggctcgtct ggacgatcgt cacgcctgtc gacccggtcg
     5581 gcaactggcg cgccggcagc tcgctgcagg cgccgccgca gacgtcgact gccttgctcg
     5641 gcgagttcga ccccttcttc cgcctcagcg gcgaggcggc gcccgtggtc gtcacctcgc
     5701 tcaacatcaa gctgttcggc atccgcgagg accgcgcctc gggccgcggc tcggcgatca
     5761 tcagcacgcc cgacggcacc cagcgcagct tcgcggtcgg cgaggagatc atccccggcg
     5821 tcaccctgac ctcggtcgcc ttcgacaatg tcaccatcac ccgcaatggc gagaccgagc
     5881 agctgttcct cgatcagtcg ccgagcgccg acgtggtggg gcctgacagc ggcgcggcga
     5941 cgccgcctcc gccgacgggc ctgctgcttc cggcctcgcc gcccagctct ccggtagcgc
     6001 cgccgccggc gccgtcgccg agcccgggcg gatcgctcgc ttcggaggcg cgcttccagc
     6061 cgcggatgaa cgggtcgcag ataaccggca tcaccgtcca gccgcagggc agcggcaacg
     6121 cgctccgcgc cgccgggctc caggcgggcg acgtcatcct gtcggtcaac gggcggcgca
     6181 ttacgtcgcc cgatcaggcg cggtcgatgg cgaaccagtt ccgcggcgag cgcgaagtca
     6241 ccgttcaggt cgagcgcgac ggacgggtca tgccggtccg cgtgaggccg ggccagtgag
     6301 gcgcgggctc ggcatcgcgc tggcgctcgt gctggccgcc ccggcggtca cccccgcctg
     6361 gtcgcaatat gtgttgaacc tgcgcgaggc cgacatccgc gcgttcatcg aggatgcggc
     6421 gcgggtcacc gggcgcacct tcgtcatcga tccgacggtg cagggcaagg tctcggtggt
     6481 gacgcagcgg ccgctgtcgc gcaccgaata tttcgaagtg ttcctctcga ctttg
//
LOCUS       DASVRK010000038        27626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_503766, whole genome shotgun sequence.
ACCESSION   DASVRK010000038 DASVRK010000000
VERSION     DASVRK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 27626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27626
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503766"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VF589_01375"
     CDS             complement(<1..892)
                     /locus_tag="VF589_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8256256.1"
                     /translation="MATAAPEASGTVAEALAHGRLLLRREPRLAAMQAREILAARPGD
                     AAALRLLGAALRKAGDQAEAEQAELEAIAASIRNPDLMAAAEALVANDLPVAERILRP
                     HLKADPFDVAAIRMMAELAARVGRFADAEHLLRRALELAPGFTASRANLATILYRQNR
                     PGEAIRVLDDLLGGDPANAAHQNLKAAALGRVGGYEEAIGLYRDVLAQHPEQPKVWMS
                     YGHVLKTVGRRQESIEAYRRAIEIAPALGEVWWSLANLKTVRFGEADIARMEAALAGD
                     GLDDDDRLHLHFALGKAHEDA"
     gene            complement(978..2687)
                     /locus_tag="VF589_01380"
     CDS             complement(978..2687)
                     /locus_tag="VF589_01380"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase"
                     /protein_id="HEX8256257.1"
                     /translation="MPATIKPVLTGNPDIDGLLEVNKWAVQSLSFGFPTAGSAYSGDY
                     AAGGEPSKNFAALTAAQQDAARAVLASIASVANLSFTEMTGAAAGKADLRIGRTDGTL
                     AGHAYTPSAHPAGGDVWFARSGDFALAAAPVAGSYAFAAMLHEIGHAVGLKHGHQAYT
                     VAVNGAARPTMSAAHDSMEYSVMTYRSYVGGPANVYSNEAAGFAQTLMMYDIAALQHL
                     YGANYASNAGNSVYGWNPATGEMSLNGAGQGAPAGNRIFMTIWDGGGVDTYSFAKYTT
                     DVTVDLRPGEWSTASTAQLAKLGVGEVAHGNIANALLYKGDARSLIENAEGGSGNDRL
                     SGNDAANALDGGDGNDLLDGKRGGDVLTGGAGNDIFIVDHAGDRLGEAAGGGTDTVRS
                     SVSFSLVRSQIEHLQLTGRADADAGGNGLANRLTGNGGANLLSGDAGNDAIAAGAGAD
                     GLFGGAGSDRLTGGTGADSFHFDAVLGRTNVDQILDFGTDDTIRLDRDIFGAIAADGT
                     LARSLFRLGSVAADRDDRILYDEASGKLFYDADGNGAGAAKLFAQLAPGTDLTNADFV
                     AYI"
     gene            complement(2892..3716)
                     /locus_tag="VF589_01385"
     CDS             complement(2892..3716)
                     /locus_tag="VF589_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX8256258.1"
                     /translation="MPLRRRSSLLLLAAAAGAGGQAVAQPPAPPPARWVVNAGEKKCE
                     LVRASNSGKELVFQLGIEPATEAPTLMVPAPKGWRGPGSMPAEVALSPRGIRIPATAL
                     RLTLDSGTVLNIHGLQPAFFDQFGAARGMAVKAGGREMASANFGGARPAVRALRECHD
                     GLLRSWGVDPKLLASLQRKPQPLKSRAALFLSGDYPIAAINRGSSGISVVRFTVGTDG
                     KPSGCVTVSPSGDEALDRASCAIMLRRGRYAPAIGADGKPVAVPQVYSVVWVIPTG"
     gene            3942..5906
                     /gene="ftsH"
                     /locus_tag="VF589_01390"
     CDS             3942..5906
                     /gene="ftsH"
                     /locus_tag="VF589_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8256259.1"
                     /translation="MSDNSNRPEKDPAGPTNPWMKSLFIWAGILLALVLFVQIIDGGG
                     RQAAGEAMGYSEFLNRVDEGSVKQVVIQKDVISGQLTNGEAFQTYAPQDPQLVNRLKE
                     KGVAFSAQPEQQTSVWLILLYQSLPFLLILGIAFFVMRQMQKNAGSGAMGFGKSKAKM
                     LTEKHGKVTFADVAGIDEAREELEEIVDFLKDPTKFARLGGKIPKGALLVGSPGTGKT
                     LLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVG
                     RHRGAGLGNGNDEREQTLNQLLVEMDGFEANEGIIIIAATNRPDVLDPALLRPGRFDR
                     QVVVPRPDIEGREKILAVHMKKVPLAPDVDPRTIARGTPGFSGADLANLVNEAALLAA
                     RKGKRLVAMSEFEEAKDKVMMGAERKSMVMTEDEKKSTAYHEAGHALVSLHVPGCDPL
                     HKVTIIPRGRALGVTWNLPERDRYSMSMKQMKARLALCFGGRIAEQIIYGEDELNTGA
                     SNDIQQATDMARAMVMEYGMSEKLGWLRYRDNQDEVFLGHSVSRTQSVSAETAQLIDQ
                     EVRRLIEEAEGIARKVLTDHLDELHRLATALLEFETLNGEESKRAIRGEDIGREDPSN
                     KRPALPTAGSSIPSTRKPRGPFGDPAPQGA"
     gene            6003..6380
                     /locus_tag="VF589_01395"
     CDS             6003..6380
                     /locus_tag="VF589_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256260.1"
                     /translation="MVRLMVVLLAAAAPLGAASAQMMPVPQFLAKAEALEKKGAMALF
                     SSDVGLLKKEISESAGQLRAERLAARKAGRKPAYCPPEKGGALGSTELLAHFRAIPPA
                     ERARMRVRDGLRSLMIRKYPCPA"
     gene            complement(6384..7490)
                     /locus_tag="VF589_01400"
     CDS             complement(6384..7490)
                     /locus_tag="VF589_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX8256261.1"
                     /translation="MNGVEGLGEAVTGGMMARAVEPQHGEGGKHAGACLNCGTPLIGD
                     YCHGCGQQGHIHRSLAAFGHDIAHSVIHFEGKIWRTLPLLAWKPGELTRRYIDGERAR
                     FVSPMALFLFSVFMMFAVFSAIGGPFDFDEGPVDRGEAARELRTERTEIAEQLQTLER
                     ELARLRAAGQPTRAVEMSIETVRAQVQTMDATARVLASGTQEEVEQAVGGIPEFSREE
                     MIGIETGWPRLNKAIEKANENPSLLFYKIQTNAYKFSWALIPISVPFVWMLFLWRRGY
                     KLYDHTVFVTYSICFMTLGLIALSLLRPIGFSEAVAGMLMTFVPPIHMYRQLRGAYRL
                     SRFSAGWRTLALLIVAPIAATFFFLLLLALGAFA"
     gene            7638..8318
                     /locus_tag="VF589_01405"
     CDS             7638..8318
                     /locus_tag="VF589_01405"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX8256262.1"
                     /translation="MNRIGLLGGSFNPAHRGHRQLSLHAARALGLDEIWWLVSPGNPL
                     KPAAGMAPLERRFASARRAARGLRVRVTAVERELGTHYTVDTIRALRRRFPDREFVWL
                     MGADNLAQFHRWKDWRKIAAAVPIAVVARPGYDGAAHAARALGWLRRFVRPAGQARKW
                     TEWRPPALVLLKLPPDPTSATALRAAEPDWHQTAGKSFNPKALRDGVTRRPLYPRRHI
                     ARTSPRSP"
     gene            8290..8685
                     /gene="rsfS"
                     /locus_tag="VF589_01410"
     CDS             8290..8685
                     /gene="rsfS"
                     /locus_tag="VF589_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX8256263.1"
                     /translation="MPVPAPAPRDGGAHDDVEALHDLVLRSLDDDQAVEVVTIPLHGK
                     STIADHMVIASGRSTRQVASMAQKLTERIKQELGRSVRIEGLPVADWVLIDANDVIVH
                     LFRPEVRSFYNLERMWAFEEKAAAAPAGA"
     gene            8750..9172
                     /locus_tag="VF589_01415"
     CDS             8750..9172
                     /locus_tag="VF589_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX8256264.1"
                     /translation="MLLHIIARGRIGRGPEAELVERYLKRIAWPTRITELPESGGKPP
                     APADNIVTILLDEKGEQLGSAAFAAKLERWRDDGRREARFLIGGADGFDDAARASADL
                     LLAFGKATWPHLLARTMLAEQLWRATSILANHPYHREG"
     gene            9177..10367
                     /locus_tag="VF589_01420"
     CDS             9177..10367
                     /locus_tag="VF589_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8256265.1"
                     /translation="MARRPLLAALLLVALAGGFAAARGQSAATSAASEAASLALAKRQ
                     ADEAALRSRQLDRQADAAESEAARARAEAAALAARIEAAEADITAAETRIRIIENLRA
                     AQRARLAERQGPVVRLTAALQTMARRPPALALVQPGSLDEVIRVRSVLASTLPVIRAR
                     TAGLREEIERGDTLRRHAETAVAELMRSREALRGRRVELARFETRQRERSQSLGQSAL
                     FESDRALAFGEEARDLAALMNTRGYQARTRARLAELPGPALRPGTPEPARGGAATAAA
                     PPYRLPVQGRLVEGMGELSDAGVHARGLTFETRANSPVVAPRAGRVAYAGPFRGYGRI
                     VIIEHGNGWTTLITNLGALQAKRGDALAAGAPIGRTGRGGPRVTVELRRQGRPFPIAP
                     LISA"
     gene            10510..11847
                     /locus_tag="VF589_01425"
     CDS             10510..11847
                     /locus_tag="VF589_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8256266.1"
                     /translation="MVKNILRPLALVSAVALIPAAATSFAAVDVDTYEEVGQFMSVFH
                     RVRSEYVEQVDDKTLLRGAIDGMLASLDPHSSYLDEKGFKSLMTTTEGNYGGLGLNVT
                     LEDGAVKVIAATEDTPAFRAGLKAGDYLTHLNGKLVYGGTLDEAVDQMRGPAGTTVKL
                     TVVRPGRDKPFDVHITRAIIELPAVKWEIKDRVGVINVNSFNKTTTDATLAAMNAIEK
                     SLGGRPLGYILDLRSNPGGLLDQAVGLSDAFLERGEIVSQRGRSKGDIERYYARPGDA
                     AHGLPVIVLVDAGSASAAEIVAGALQEHHRAIVMGERTFGKGSVQTLLPLSEQTALRL
                     TTARYFTPSGKSVQEGGITPDIAVPQLSDPDYKDRPVFRESDLRRHLVNEIAVKDDVV
                     QDDGKPDPRFTATAEQLKKQGVEDFQLHYALQTLSRLGKVQQAAAGQPKAGSR"
     gene            11961..12473
                     /locus_tag="VF589_01430"
     CDS             11961..12473
                     /locus_tag="VF589_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX8256267.1"
                     /translation="MPQKASPLTALDKARWLALIVPAALLAGALGSQYLGGLHPCEMC
                     HWQRWPHYAAVALALVAFAIRGAADRGRSFVWLAALAILTSGAIGVYHAGVEAGIFEG
                     LTQCTASAAGGSSADILADIMANPLVRCDQVQFSFLGVSMAGWNAIVSIGSALVILWL
                     SLKRPRVAAA"
     gene            12470..13018
                     /locus_tag="VF589_01435"
     CDS             12470..13018
                     /locus_tag="VF589_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX8256268.1"
                     /translation="MTDRRSDWRPGDDRADVASMIRVDQAGEYGATRIYAGQLAVLGN
                     RHPAARVIARMASQEERHLNHFDQLLADRGIRPTILQPFWNVAGHALGAVTALMGPDA
                     AMACTAAVETEIDKHYADQLRALGDQDPDLSATITEFQAEELEHRDTALAAGAENTFG
                     YPVLSAFIRAGCRVAIELSKRI"
     gene            13022..13504
                     /locus_tag="VF589_01440"
     CDS             13022..13504
                     /locus_tag="VF589_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256269.1"
                     /translation="MRYTLLAALLLAGPAAAQEAAPAAGPTDAKVSQLIVYGDDPCPQ
                     SSADEIVVCARKPEGDRFRIPEELRANPNDPANQSWTNRASELQYVGRAGIGSCTPVG
                     PGGGTGCYAELVRQARAERAGRESVNWNRLVEQARQERLGRIDAEAEAEEAEAVARGE
                     "
     gene            13604..14011
                     /locus_tag="VF589_01445"
     CDS             13604..14011
                     /locus_tag="VF589_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256270.1"
                     /translation="MKFVLAAPLLAAVAASALSAQSPADQQRRQVRQVIVYGTDPCPR
                     STGDEIIVCSRRPETERYRIPEGVRDETDDDDPDNESWAERARSLDEPARAMTGPGAC
                     EPVGPGHHTGCTTEMLNAHRAAREAEEAEGPER"
     gene            complement(14208..15953)
                     /locus_tag="VF589_01450"
     CDS             complement(14208..15953)
                     /locus_tag="VF589_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8256271.1"
                     /translation="MRGILIALLLAGSAPAAAAPADDLKRLLDEHWASTMRENPVFAT
                     LVGVRDHEGRVPDVSLAAADRRTREAQALLARLEAIPAAGLSAADRTNRAILKRMLEE
                     QIEANRHGQRMILYSNSGGWHQSTADMASVLPFRTAADYDSYLKRLEQYPKINDEALS
                     ITARAVKEGYVLPCAAMAGHDKTISGTIAGAPDKSRFYEPFTRAKPTDVSAADWAAMQ
                     GRARTLIEATLYPAYRKHLAFYTSEVAPKCAKAVGVSAQPGGRDYYNFRIRQMTTTGL
                     KADEIHAIGLKEVARIRAEMEAVAKKAGFATREAYIADLRTNPKYYAKTPDELMRETA
                     YVLKQIDGKMPSLFGKLPRLPYGIRAIPAETAEGTTTAYYNFGSLENGIAGTYYVNTS
                     KLDQRPLWEIPALSVHEAVPGHHQQIALQQELDLPNMRKYLAFFTGFVEGWGLYSESL
                     GEEMGLYDTPAKEMGKLSYQMWRATRLVVDTGMHSKGWSKERAVGYMKDNTALSEANI
                     EAEVNRYISDPGQALAYKIGELKIRELRARAEKALGAKFDLREFHDVVLGQGAVPLDV
                     LEAQVDEWIAAEQRA"
     gene            complement(16097..17848)
                     /locus_tag="VF589_01455"
     CDS             complement(16097..17848)
                     /locus_tag="VF589_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8256272.1"
                     /translation="MRRSFVLAAALALAAPAAAAPASDFGQLLEDHYAWLLRESPVQA
                     TALGVRDHDARIDDYSPAARDRRGEEARGFLARLNAIPAAGLSAADRTNRAILKRMLE
                     EQIEANRYGQRTMLFTTYYGWHQGFAGMANGLPFRTKADFESYLTRLGQYPKLNDQAL
                     AVTAEAVRGGYVLPCSVLGNYEKGIAGVIAEDAAKSRFFEPFAGERPVDVSAAEWTQM
                     QARARRIIAEVLNPAYAKHAAFYRSQYAPRCAKADSISAQPGGREYYAFRVRQETTTD
                     LTPDQIHTIGLAEAKRIRAEMEAVAKQAGFASREAFIRELRTNPQYYARTPEELMEAS
                     ARVAKAIDGKMPAFFKTLPRLPYGIREIPAEIAEGTTTAYYGSGSPESGIAGTYYVNT
                     SKLDQRPLWEIPALSLHEAVPGHHHQISLAQELEIAPFRRNFTSFTAFTEGWGLYAES
                     LGAAMGLYDTPAKKMGQLSYQMWRAARLVVDTGIHAKGWDKARAVAFMRENSALSEAN
                     IDAEVNRYISWPGQALGYMIGNIRIRELRAKAEKALGARFDLRRFHDAVLLQGSVPLD
                     VLEAQVDEWLAAERGRS"
     gene            17968..18177
                     /locus_tag="VF589_01460"
     CDS             17968..18177
                     /locus_tag="VF589_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEX8256273.1"
                     /translation="MNDNVYKIIEVVGSSATGIEDAIEKAIARASASLHDIRWFEVKE
                     TRGHVENGRVAHYQVTLRIGFTLRD"
     gene            18453..19868
                     /locus_tag="VF589_01465"
     CDS             18453..19868
                     /locus_tag="VF589_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX8256274.1"
                     /translation="MTLAEATPELDIARNDRNDRNDRAFLGHPKGLGYLAFTETWERF
                     SYYGMQTLLVLYMVNELLLPGHVENIAGFEAFRSLYGDIDGQKLASAIFGTYTSLVYL
                     TPILGGLLADRVLGKRRTVMLGAITMAAGHFLMAFETSFLLALLCLILGNGMFKGNIA
                     SQVGALYGPDDLRRATAFQIFFLGINAAVIVSPLVVGTLGEQVGWHWGFGAAGVGMLI
                     GLGVYIAGQKHLPEEHFDRRAGAGARAERPRLTATDWSALIALFLLIPVMAIGFLPNQ
                     QIFNAYLVWGDRQFDLTFMGTRLPTSWLITLDAITSVSFLAIVALFYSWYGKRRREPD
                     EVTKMIIGSVFSIGGMLCLFMAASTTAEGEKIGLFWPVMFHLLNSIGFAHMLPVSLAL
                     FAKVAPKAVNATVIGIYYLTFVAANGLVGYVGGWLETMPATTFWLMHAGFAAAAGAVF
                     ILFKLFLSKRLVQSEPAPLPD"
     gene            20087..20581
                     /locus_tag="VF589_01470"
     CDS             20087..20581
                     /locus_tag="VF589_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX8256275.1"
                     /translation="MQNFQVRSPDSVPSPPKADSAADKAERPQMSMIGADIIITGNIE
                     ASVDLHIEGRVMGDVRCSTLILGESSIINGGIHADRVRVSGMVEGAIVTRDLAIEATA
                     RVTGEVSYERLRIANGAIVDGTLKRNAPPEASADARLKLVEAKAEAKLQPKLEAEPAV
                     IVIE"
     gene            20617..20976
                     /locus_tag="VF589_01475"
     CDS             20617..20976
                     /locus_tag="VF589_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256276.1"
                     /translation="MLSQQMTIVVVPPEPDLTPATAFEELEAILYTVAASPPQHRKFA
                     VRYMQCRDVLMGSELRATLPGFMLQTGTIDKFRDFITLYDRDVPARRAFLDHALGRCR
                     ALVGPQRVYDDFADLDF"
     gene            20978..21649
                     /locus_tag="VF589_01480"
     CDS             20978..21649
                     /locus_tag="VF589_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256277.1"
                     /translation="MRLWCALAGHAPDAGLATWNLGYCFSRCERCGRDIVRTGEGRWK
                     VPHGYRIVWQPLPAKAADPPEPAEAELPPAAESEPLPWGEVADPVHFDAAPEAEPLLT
                     EPEPLPPEKVAAEPEPIPFEAAPEIAPSEDEPPAPAIEPGPPAPVEDELPLPPAGIEE
                     EEAPLPQAAIEVEAPLPQPERARDFMDMDVEEEDWDDLAALPPAAMAAAAPAAAAPRW
                     LALPD"
     gene            complement(21654..22967)
                     /locus_tag="VF589_01485"
     CDS             complement(21654..22967)
                     /locus_tag="VF589_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8256278.1"
                     /translation="MNALSRIDAVTAADPRPRSAVSVGVGVAGLAGLGGWAIFAGSYG
                     LDGPGYAMVALVACGLPMVLWSLLVDKVHRSPSTGIDWDSPPRPLRESADISLAKIVG
                     LWGIWAAIAFLYCVGRWYWDGQYLFAMQALAVAALPMLILSVPYVVWLDRRLKEPRDG
                     AWHFGRLIIGRTDGLDRELLYDFFRAWAVKGFFLAFMISIVPGNWTDTVRPTLSDILA
                     SPVTLTHWLVGAMFLIDVAFATVGYMLTMKPLDAHIRSANPYAAGWTAALICYPPFIL
                     MNPGGPLNYQQGAGEWTHWFAGSPLALGVIGAVLVLLTAYYAWATVAFGIRFSNLTHR
                     GILTHGPYAWTKHPAYVSKNLFWWLATMPFLAASGNPVDAVRNTVVLGLVSGVYYWRA
                     RTEERHLMADPAYRDYAAWMERNAPLPRLLARIRRRPAPEPLAAE"
     gene            23172..23792
                     /locus_tag="VF589_01490"
     CDS             23172..23792
                     /locus_tag="VF589_01490"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8256279.1"
                     /translation="MNLAYEETTFSFSSEAPGPQERRRDTRHMTILRVGTLIVDGARE
                     LCLIRNISAGGLMAHVYSQLDLGKAVAVELKTNQQIAGRVSWIKGGNIGIAFDQIIDV
                     EELLASQNALDGGWRPRLPRVEVDRMGTLRAGARTHWVSTRDISQGGAKLDTDQPLDP
                     GTDIVLTLEGFRPLAGVVRWSHEGLCGVSFNQVIPFGELMGWLRQA"
     gene            complement(23798..24508)
                     /locus_tag="VF589_01495"
     CDS             complement(23798..24508)
                     /locus_tag="VF589_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX8256280.1"
                     /translation="MTSAGAAAAFHLLLGYALIGGLGVEVSRQAEERLKLFEVREAPP
                     PPEVKAAPAPKLEKATEAAAAPAAPAASPVLAPPPEVRVEAAPPLPAAPVVGSGVGVL
                     AGSSSGSGVGTGSGGAGSSTGSGGGGGVARSARLIGGALLDSDYPRAARRKRAEGVVF
                     LSFKVAADGGVSDCIVTRSSGHAELDATTCRLIERRFRYAPALDAEGKKVPDLVVGRH
                     AWWIGRRRPGGAGTEGRK"
     gene            complement(24607..24927)
                     /locus_tag="VF589_01500"
     CDS             complement(24607..24927)
                     /locus_tag="VF589_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256281.1"
                     /translation="MIKRLGPNRDAHSPDNPGSHGAAGDLARGDPEAMDKFNGRESGL
                     GGGDSGGGAYPNPHTGKSAKNGGFMGHGGQTEMPYHGGGQLGEQETGGNENAPAKKTG
                     KRQD"
     gene            complement(24972..26264)
                     /locus_tag="VF589_01505"
     CDS             complement(24972..26264)
                     /locus_tag="VF589_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8256282.1"
                     /translation="MVETARFDRAHRLRNIIGGSAGNLVEWYDWYVYSAFTLYFAPVF
                     FPAGDRTAELLSAAAVFAVGFLMRPIGAWAMGIYADRKGRKAGLTLSVALMCGGSLLI
                     AVTPTHAAIGAWAPAILVAARMLQGLSVGGEYGASATYLSEMAGRERRGFFSSFQYVT
                     LISGQLIALAVLILLQAVMSEAALEAWGWRIPFAIGAALAVVVFWIRRRLTETESFEN
                     ARDGGAPKASGWGLFRDHPREALLVMALTAGGTLAFYAYTTYMQKFLVNTSGFSRQTA
                     TQVTAAALFLFMLLQPAAGALSDRVGRRPLMIGFGLLGLFLTYPIFRTLETVRDPFLA
                     FLLVFGALLIVTGYTSINAVVKAELFPAHTRALGVALPYALANTLFGGTAEYAALWFK
                     DAGVERAFYWYVTGMIGLSLIVYWRMRDTREHSLILED"
     gene            complement(26374..26784)
                     /locus_tag="VF589_01510"
     CDS             complement(26374..26784)
                     /locus_tag="VF589_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256283.1"
                     /translation="MAEARPVLARLPARPAVILVWLCSAPATAAPPEEESGDYMAVAA
                     ETFRVERGCGPVGEGQDDEIVVCRRRDNEKYRLPIRENRFDPGGATDSVSRERHRLIE
                     EGDAGIGSCSTVGPGGYTGCFPQNIKRRQQQEGE"
     gene            complement(26950..27417)
                     /locus_tag="VF589_01515"
     CDS             complement(26950..27417)
                     /locus_tag="VF589_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256284.1"
                     /translation="MISRPDGSTSLAIAWILFAASLLFALVAYSMDVTTYSGIDPGKV
                     ALQFALWVLAGALFQLWLVLWAVGSIVRALWFLPGELQKQPEAPQADRFAASPTPQEM
                     EGQVCGWCDRRVREPGLPCSEATEEQRRAAFKAIQNPMCRARLQEHGYGEPAA"
     gene            complement(27410..>27626)
                     /locus_tag="VF589_01520"
     CDS             complement(27410..>27626)
                     /locus_tag="VF589_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HEX8256285.1"
                     /translation="PLAWPVGLVAILGCIYLFLSLPQTTQIYFLIWNVIGLVFYMLYG
                     SRRSAAGAAGPGIIVDEPRKKGVREND"
BASE COUNT         4438 a        10107 c         8845 g         4236 t
ORIGIN      
        1 cggcatcctc atgagccttg ccgagcgcga aatggagatg gaggcggtcg tcatcgtcga
       61 ggccgtcgcc cgcgagcgcc gcctccatcc gggcaatgtc cgcctcgccg aacctgaccg
      121 tcttcaaatt ggcgaggctc caccacactt cgcccagagc aggcgcgatt tcgatggcgc
      181 ggcgataggc ctcgatgctc tcctgccggc gcccgaccgt cttcagcaca tggccgtagc
      241 tcatccacac cttgggctgt tcgggatgct gggcgagcac gtcgcgatag aggccgatcg
      301 cctcctcata gccgccgacc cggccgagcg ccgccgcctt gagattctgg tgggcggcat
      361 tggcgggatc cccgccgagc aggtcgtcga gcaccctgat cgcctcgccc ggccggttct
      421 gccggtagag gatggtggcg aggttcgcac gcgacgccgt aaagccgggg gcgagctcga
      481 gcgcgcggcg cagcagatgc tcggcgtcgg cgaagcggcc gacgcgggcg gcgagctccg
      541 ccatcatccg gatcgcggcg acgtcgaacg ggtctgcctt gaggtgcggg cgcaggatcc
      601 gctccgcaac cggcagatcg ttcgccacca gcgcctcggc cgcggccatc aaatcggggt
      661 ttcggatcga ggcggcgatc gcctccagct ccgcctgctc ggcctcggct tggtcaccgg
      721 ccttgcgaag cgcggccccg agcaggcgca aagcggccgc atcgccgggg cgggcggcga
      781 gaatctcgcg ggcctgcatc gccgccagcc gcggctcgcg ccggagcagc agccgcccgt
      841 gcgcgagcgc ttcggcgacc gtgccggaag cctcgggtgc cgctgtcgcc atcatcgatc
      901 ccgtcgctct ccccgctcaa tgcccatgct aggcaggata gctgcggggc gaaacccccc
      961 ggagacgcga ggccgcctca gatgtaggcg acgaaatccg cgttggtcag gtcggtgccg
     1021 ggggcgagct gcgcgaacag cttggccgcg cccgcgccgt tgccgtcggc gtcgtaaaac
     1081 aatttgccgg acgcctcatc gtaaaggatg cggtcgtcgc ggtccgccgc cacgcttccg
     1141 agccggaaga ggctgcgcgc gagcgtgccg tcggccgcaa tggcgccgaa aatatcgcga
     1201 tcgaggcgga tcgtgtcgtc cgtcccgaaa tcgaggatct ggtcgacatt ggtgcgcccc
     1261 agcaccgcat cgaaatggaa ggaatcggcc ccggtcccgc cggtcaggcg gtcgctgccg
     1321 gcgccgccga acaggccgtc ggcaccggcg ccggctgcga tcgcgtcatt gcccgcatcg
     1381 ccgctcagca ggttcgcgcc gccattgccg gtgagccggt tggcaaggcc gttgccgccc
     1441 gcatccgcgt cggcgcggcc ggtgagctgc agatgctcga tctggctgcg gaccagcgag
     1501 aagctgaccg agctgcgcac cgtgtcggtg ccgccgcccg ccgcctcgcc gaggcggtcg
     1561 ccggcatgat cgacgatgaa gatgtcgttg ccggcgccgc cggtgaggac gtcgccgccg
     1621 cgcttgccgt cgagcagatc gttgccgtcg ccgccatcga gcgcgttggc ggcgtcgttg
     1681 ccgctcagcc ggtcgttgcc gctgccgccc tcggcattct cgatcagcga gcgcgcgtcg
     1741 cctttgtaaa ggagcgcgtt ggcgatattg ccgtgggcga cctcgccgac gccgagcttg
     1801 gcgagctgcg ccgtggaagc ggtgctccac tcgccgggcc tgagatcgac ggtgacgtcg
     1861 gtggtatatt tggcgaagct gtaggtatcg acgccgccgc cgtcccagat cgtcatgaag
     1921 atgcggttgc cggccggcgc gccctggccg gcgccgttga ggctcatctc gccggtggcg
     1981 ggattccagc cgtagacgct gttgccggca ttgctggcgt aattggcgcc gtaaagatgc
     2041 tggagcgcgg cgatgtcgta catcatcagg gtctgcgcga agcccgccgc ctcgttgctg
     2101 tagacgttgg ccggaccgcc cacataggag cggtaggtca tcaccgaata ttccatgctg
     2161 tcatgagcgg cgctcatggt cgggcgggcg gcgccgttga ccgcgaccgt ataggcctgg
     2221 tggccgtgct tgagcccgac ggcatggccg atctcgtgca gcatcgcggc aaaggcatag
     2281 ctgccggcga ccggggcggc ggcgagcgcg aagtcgccgc tgcgcgcgaa ccagacgtcg
     2341 ccgccggccg ggtgggcgct cggcgtgtag gcgtggccgg ccagcgtgcc gtcggtgcgg
     2401 ccgatgcgca ggtcggcctt gcccgccgcc gcgccggtca tttcggtgaa gctgagattg
     2461 gcgaccgacg cgatgctcgc cagcaccgcc cgcgcggcat cctgctgggc ggcggtgagg
     2521 gccgcgaaat tcttggacgg ctcgccgccg gcggcatagt cgccgctata ggcggagccg
     2581 gcggtcggga agccgaagct cagcgactgc accgcccatt tgttgacctc gagcagcccg
     2641 tctatatccg ggttgcccgt cagcaccggt ttgatcgtgg ccggcatatc attctccccc
     2701 cgcaggcccg tagcgccgcc gtcttaggcc tcccaagctt gcgcaaaggt taccggcggg
     2761 cgctctcggc gcccttcgta cgggcgcggc gcgaacggcc ataatgccac gccgggccta
     2821 ggggtggcaa tcccgccggc ggatatactc aaggcgcggg cggcggagag ggcgacgcga
     2881 cgtaaaccgc ctcaccccgt ggggatcacc cacaccacgc tatagacctg cggcacggcc
     2941 acgggcttgc cgtcggcgcc gatcgcgggc gcgtagcgcc cccgcctcag catgatggcg
     3001 cagctcgcgc ggtcgagcgc ttcatccccg ctcggcgaca cggtgacgca gccggaaggc
     3061 ttgccgtcgg tgccgaccgt gaaacgcacc acgcttatcc ccgagctgcc ccggttgatc
     3121 gccgcgatcg ggtaatcgcc ggacagaaac aacgccgccc ggcttttgag cggctgcggc
     3181 ttgcgctgga gcgaggccag cagctttgga tccacgcccc aggaacggag caggccgtcg
     3241 tggcactcgc gcagcgcccg gaccgctggc cgcgctccgc cgaaattcgc ggacgccatc
     3301 tcccggccgc ccgccttcac cgccatgccc ctcgcggcgc cgaactggtc gaagaaggcc
     3361 ggctgcaagc cgtggatgtt cagcaccgtt ccgctgtcca gcgtcagccg aagggcggtc
     3421 gccgggatcc gtatccctcg gggcgaaagc gccacttcgg ccggcatcga gcccggcccc
     3481 cgccacccct tcggggcggg caccatcagc gtcggcgcct cggtcgccgg ctcgatcccc
     3541 agctggaaca ccagctcctt gccgctgttc gaagcccgca ccagctcgca cttcttctcg
     3601 cccgcattca ccacccaccg cgccggcggc ggcgcgggag gctgggcgac ggcttgcccg
     3661 ccagctccgg ccgcggcggc aagcaggagc agcgacgatc ggcgccgaag aggcatcttc
     3721 acgaccatgg cacgcatttg gttgcagaga gcgacttcca gcgcaagcgc ccgcgctccc
     3781 aaggccgcag cgacggcggc ccgacccatt tatcggccgt tcacccgcaa tggctacatt
     3841 gtcgagccgc gcgccagcgc ctatcttggg gcggcaaagg gaagtgacgt tcgaagggcc
     3901 catgcgggta gccatcgaaa taccacttcg atggagagac gatgagcgac aattcgaacc
     3961 gccccgagaa ggacccggca gggcccacca acccctggat gaagagcctg ttcatctggg
     4021 ccggcatcct gctcgccctg gtcctgttcg tgcagatcat cgacggcggc ggccgccagg
     4081 cggcgggcga ggcgatgggc tattcggaat tcctgaaccg ggtcgatgaa ggctcggtca
     4141 agcaggtcgt catccagaag gacgtgatct ccggccagct caccaacggc gaggccttcc
     4201 agacctatgc gccgcaggac ccgcagttgg tcaaccgctt gaaggagaag ggcgtcgcct
     4261 tttcagccca gcccgagcag cagaccagcg tctggctgat cctcctctat cagtcgctgc
     4321 ccttcctgct gatcctcggc atcgccttct tcgtgatgcg ccagatgcag aagaatgccg
     4381 gctccggcgc gatgggcttc ggcaagtcca aggccaagat gctgaccgag aagcacggaa
     4441 aggtgacctt tgccgacgtc gccggcatcg acgaagcgcg cgaggagctg gaggagatcg
     4501 tcgatttcct caaggacccg accaaattcg cgcggctcgg cggcaagatc cccaagggcg
     4561 cgctgctggt cggctcgccc ggcaccggca agaccttgct cgcccgcgcc atcgcgggtg
     4621 aggcgaacgt gcccttcttc actatttcgg gctcggactt cgtcgagatg ttcgtcggcg
     4681 tcggcgcgag ccgcgtccgc gacatgttcg agcaggccaa gaagaacgcc ccctgcatcg
     4741 tcttcatcga cgagatcgac gcggtgggtc gtcatagggg cgccggcctc ggcaacggca
     4801 atgacgagcg cgagcagacg ctgaaccagc tgctggtcga gatggacggc ttcgaggcca
     4861 acgagggcat catcatcatc gccgccacca accgccccga cgtgctcgat ccggcgctgc
     4921 ttcggcccgg ccgcttcgac cgccaggtcg tggtcccgcg cccggacatc gagggccgcg
     4981 agaagatcct cgcggtgcac atgaagaagg tgccgctcgc ccccgacgtc gacccgcgca
     5041 ccatcgcccg cggcacgccc ggcttctccg gcgccgacct cgccaacctc gtcaacgagg
     5101 ccgctttgct cgccgcgcgc aagggcaagc gcctcgtcgc gatgagcgag ttcgaggagg
     5161 ccaaggacaa ggtcatgatg ggcgccgagc gcaagtccat ggtcatgacc gaggacgaga
     5221 agaagtcgac cgcctatcac gaggccggcc atgccttggt ctcgctccac gtccccggct
     5281 gcgacccctt gcacaaggtc accatcatcc cgcgcggccg cgcgctgggc gtgacctgga
     5341 acctgcccga gcgcgaccgc tattcgatgt cgatgaagca gatgaaggcg cggctcgcgc
     5401 tctgcttcgg cggccggatc gccgagcaga tcatctatgg cgaggacgaa ctcaacaccg
     5461 gcgcttccaa cgacatccag caggccaccg acatggcccg cgcaatggtg atggaatatg
     5521 gcatgtcgga aaagctcggc tggctcaggt accgcgacaa ccaggacgag gtgttcctgg
     5581 gccattccgt ctcgcgcacc cagtcggtct cggccgagac cgcccagctg atcgaccagg
     5641 aggttcgccg cctgatcgaa gaggccgagg ggatcgcgcg caaggtgctc accgaccatc
     5701 tcgacgagct ccaccgcctt gccaccgctt tgctcgaatt cgagacgctg aacggcgagg
     5761 aatcgaagcg ggccatccgg ggcgaggata tcggccgcga ggacccctcc aacaagcgcc
     5821 ccgccctgcc gaccgccggc tcgtcgatcc cgagcacgcg caagccgcgc gggccgttcg
     5881 gagaccccgc cccgcaaggc gcctgatccg gctaaccgct tgcaatgagc ggcgaacctg
     5941 cgatggatga acgggagccg gtccgctgtc cggctcccgt tttcatgctc gggggaggaa
     6001 caatggtccg tctgatggtg gttttgctcg cggctgcggc tccgctcggc gcggcgtccg
     6061 cgcagatgat gccggtgccg caattcctcg ccaaggccga ggcgctcgag aagaagggcg
     6121 cgatggcgct gttctcgagt gacgtcggcc tgctcaagaa ggaaatcagc gagtccgcgg
     6181 gccagcttcg cgccgaacgg ctcgccgcgc gcaaggccgg ccgcaagccc gcttattgcc
     6241 cgcctgaaaa gggcggcgcg ctcggcagca ccgaattgct ggcgcacttc cgcgcgatcc
     6301 cgccggcgga gcgcgcccgg atgcgcgtgc gcgatggcct gcgcagcctg atgatccgca
     6361 aatatccctg ccccgcctga ccgtcaggcg aacgctccca aagcgagcag cagcaggaag
     6421 aagaaggtcg ccgcgatcgg cgccacgatc agcagcgcca gcgtccgcca ccccgcactg
     6481 aaccgcgaca gccgataggc gccgcgcagc tgccggtaca tgtggatcgg cggcacgaac
     6541 gtcatcagca tgccggcgac cgcctcgctg aagccgatcg gccgcaacag gctcagcgcg
     6601 atcagcccca acgtcatgaa gcagatcgaa taagtgacga acacggtgtg gtcgtaaagc
     6661 ttgtagccgc gccgccagag gaacagcatc cagacgaacg gcaccgagat cgggatcagc
     6721 gcccacgaga atttataggc gttggtctgg atcttgtaga acagcagaga cggattctcg
     6781 ttggccttct cgatcgcctt gttgagccgc ggccagccgg tctcgatccc gatcatctcc
     6841 tcgcgggaaa attcgggaat gccgcccacc gcctgctcga cttcctcctg ggtgccggac
     6901 gcgagcacgc gggcggtggc gtccatcgtc tggacctgcg cgcgcaccgt ctcgatgctc
     6961 atctcgaccg cgcgggtcgg ctgcccggcc gcgcgcaacc gggcgagctc gcgctcgagc
     7021 gtctggagct gctcggcgat ctcggtccgc tccgtcctca gctcgcgcgc cgcttcgccg
     7081 cggtcgaccg gcccctcgtc gaaatcgaac gggccgccga tggcgctgaa cacggcgaac
     7141 atcatgaaca ccgagaacag gaacagcgcc atcggcgaga cgaaccgcgc ccgctcgccg
     7201 tcgatatagc gccgcgtcag ctcgcccggc ttccaggcga gcagcggcag ggtccgccag
     7261 atcttgcctt cgaagtggat cacgctgtgc gcgatgtcgt ggccgaaggc ggcgaggctg
     7321 cggtggatat ggccctgctg gccgcagccg tggcaataat cgccgatcag cggagtgccg
     7381 caattgaggc aggcgccggc atgcttgccg ccctcgccat gttgcggctc gaccgcccgg
     7441 gccatcatcc cgccggtcac cgcctcgcct agaccttcca ccccgttcac gccgccgccc
     7501 tgcgttgaag cgaagctcgt aatgccctac aatcgcgcga tgagcaaacg ggcggattgc
     7561 atcctcctcc acacgtccgt ccgtcacgag atcgtcattg cgagggtgcg gaacgccagg
     7621 gaagactggc ggcggctttg aaccgcatcg gccttctcgg cggctcgttc aatccggccc
     7681 atcgcgggca ccggcagctc agcctccacg ccgcccgcgc gctcgggctc gacgagatct
     7741 ggtggctggt ctcgcccggc aatccgctga agccggccgc cggcatggcc ccgctcgagc
     7801 ggcgcttcgc ctccgcccgc cgcgccgccc gcggcctcag ggtccgggtg acggctgtcg
     7861 agcgcgagct tggcacccat tatacggtcg acaccatccg cgcgctccgc cgccgcttcc
     7921 ccgaccgcga gttcgtctgg ctgatgggcg ccgacaatct cgcccagttc caccgctgga
     7981 aggactggcg caaaattgcc gccgccgtgc cgattgcggt cgtcgcccgg ccgggatatg
     8041 atggcgccgc ccacgcggca cgcgcgctgg gctggctgcg ccgcttcgtc cggcccgcag
     8101 gccaggctcg caagtggacg gagtggagac cgccggcgct cgtgctgctc aaattgccgc
     8161 ccgacccgac ttccgccacc gccctgcgcg ctgccgagcc cgactggcac caaacggccg
     8221 gtaaatcgtt caacccgaag gcgctccgcg acggcgtgac gcggcggcct ttgtacccaa
     8281 ggaggcacat tgcccgtacc agcccccgct ccccgtgacg gcggagccca tgacgatgtc
     8341 gaagccctgc acgatctcgt gcttcgctcg ttggatgacg accaggccgt agaagtcgtc
     8401 accatcccgc tccacggcaa gtccaccatc gccgaccaca tggtgatcgc ctccgggcga
     8461 tcgacccgcc aggtcgcgtc gatggcgcag aagcttaccg agcggatcaa gcaggagctc
     8521 ggccgcagcg tccgcatcga aggcctgccg gtcgccgact gggtgctgat cgacgccaat
     8581 gacgtcatcg tccacctctt ccggccggag gtgcgcagct tctacaatct ggagcggatg
     8641 tgggccttcg aggagaaggc cgccgcggcc cccgcgggcg cctgagccgg ccgccgtccc
     8701 cggcgccttc gccatgccgg tatctcagag atgaccagct aggcctcgga tgctgctcca
     8761 catcatcgcg cgcggccgga tcgggcgcgg ccccgaggcc gagctggtcg agcgctatct
     8821 gaagcgcatc gcctggccga cccggatcac cgagctgccc gagagcggcg gcaagccgcc
     8881 cgcgcccgcc gacaatatcg tcaccatcct gctcgacgag aaaggcgagc agctcggatc
     8941 ggcggccttc gccgccaagc tggagcgctg gcgcgacgac ggcaggcgcg aggcgcgctt
     9001 cctgatcggc ggcgccgacg gcttcgacga cgccgcccgc gcctccgccg acctgctgct
     9061 cgccttcggg aaagcgacct ggccgcacct gctcgcccgc acgatgctcg ccgagcaatt
     9121 gtggcgcgcg acatcgatcc ttgccaacca cccctatcat cgcgaaggct agcgccatgg
     9181 cccggcgccc gctccttgcc gctctgctcc tcgtcgccct tgccggcggc ttcgcggccg
     9241 cgcgtggcca gagcgccgcc accagcgccg cctccgaggc cgcttcgctc gcgctcgcca
     9301 agcgccaggc cgacgaggcg gcgctccgct cccgccagct cgatcgccag gccgacgcgg
     9361 ccgaaagcga agccgcccgc gcccgcgccg aggccgccgc gctcgcggcc aggatcgagg
     9421 ccgccgaagc cgacatcacc gccgccgaga ctcgcatccg catcatcgag aatctgcgcg
     9481 ccgcccagcg cgctcgcctg gccgagcggc agggcccggt ggtccggctc accgccgcgc
     9541 tccagaccat ggcccggcgc ccgcccgcgc tcgccctggt ccagcccggc tcgctcgacg
     9601 aggtgatccg cgtccgctcg gtgctggcct cgaccctgcc ggtgatccgc gcccgcaccg
     9661 cgggccttcg cgaggagatc gaacgcggcg acacgctccg ccgccacgcc gagacggcag
     9721 tcgccgagct gatgcgcagc cgcgaggcgc tgcgcggccg ccgcgtcgag ctcgcccgtt
     9781 tcgagacccg ccagcgcgag cgctcgcaaa gcctcggcca gtccgctctg ttcgaatccg
     9841 accgggcgct ggcgttcggc gaggaagcgc gcgacctcgc cgctttgatg aacactcgcg
     9901 gctaccaggc ccgcacccgc gcccgcctgg ccgagctgcc cggcccggcg ctccgcccgg
     9961 gaacgcccga gcccgcgcgc ggcggcgccg caacggccgc cgctccgccc taccgactgc
    10021 cggttcaggg ccggctggtc gaaggcatgg gcgagctttc cgacgccggc gtccacgccc
    10081 gcggcctcac cttcgagacc cgcgccaaca gccccgtggt cgcgccgcgc gccggccgcg
    10141 tcgcttatgc cgggccgttc cgcggctatg gccggatcgt catcatcgag catggcaatg
    10201 gctggaccac gctcatcacc aatctcggcg cgctccaggc caagcgcggc gacgcgctcg
    10261 cggccggcgc accgatcggc cgcaccgggc gcggcgggcc ccgcgtcacg gtcgagctgc
    10321 gccgccaggg ccggccgttc ccgatcgccc ccttgatctc cgcctgatgc gccgcgcgcg
    10381 ccccgacccg ccaagcgccc gacgatccgc tccgaacttg cgggcggatg aacggcggtt
    10441 aaccctgcgt tccttttcga gcttctaaaa agcctctaca tagtcgattc ccgtcagcag
    10501 gatttcccca tggtcaagaa catccttcgc ccgctcgctc tcgtcagcgc ggtcgccctc
    10561 atccccgccg ccgccacctc gttcgccgcc gtcgacgtcg acacctatga ggaagtcggg
    10621 cagttcatga gcgtcttcca ccgcgtccgc tccgaatatg tcgagcaggt cgacgacaag
    10681 actttgctgc gcggcgccat cgacggcatg ctcgccagcc tcgatccgca cagctcctat
    10741 ctcgacgaga agggcttcaa gtcgctgatg acgacgaccg agggcaatta tggcggcctc
    10801 ggcctcaacg tcaccttgga ggacggggcg gtcaaggtga tcgccgcgac cgaggacacg
    10861 cccgccttcc gcgccggcct caaggcgggc gactatctca cccatttgaa cggcaagctc
    10921 gtctatggcg gcacgctcga cgaggccgtc gaccagatgc gcggccccgc gggcaccacc
    10981 gtcaagctga ccgtggtccg gcccggccgc gacaagccgt tcgacgtcca catcacccgc
    11041 gccatcatcg agcttcccgc cgtcaagtgg gagatcaagg acagggtcgg cgtgatcaac
    11101 gtcaacagct tcaacaagac caccaccgac gccaccctgg ccgcgatgaa cgcgatcgag
    11161 aaatcgctcg gcggccgccc gctcggctac attctcgacc ttcgctccaa tccgggcggc
    11221 ctgctcgacc aggcggtcgg cctcagcgac gccttcctcg agcgcggcga gatcgtctcg
    11281 cagcgcggcc gctccaaggg cgatatcgaa cgttattatg cgcggcccgg cgatgccgcc
    11341 cacggcttgc cggtaatcgt actggtcgac gccggctcgg cctccgcggc cgagatcgtc
    11401 gccggcgcgc tccaggagca tcaccgcgcc atcgtgatgg gcgagcgcac cttcggcaag
    11461 ggctcggtgc agaccttgct gccgctgtcc gagcagaccg cgctccggct caccaccgcc
    11521 cgctatttca cgccttcggg caagtcggtg caggaaggcg ggatcacgcc cgacatcgcg
    11581 gtgccgcagc tttcggaccc cgattacaag gaccggccgg tgttccgcga atccgacctt
    11641 cgccgccacc tcgtcaacga gatcgcggtc aaggatgacg tcgtccagga cgacggcaag
    11701 cccgatccgc gcttcaccgc caccgccgag cagctcaaga agcagggtgt cgaggacttc
    11761 cagctccact acgcgctcca gacgctgtcg cggctcggca aggtccagca ggccgccgcc
    11821 ggccagccca aggccggctc acgctaaaca aaattcctcc cctgccttcg caggggaggg
    11881 gaccgcgcaa agcgcggtgg aggggcggcg cgaagcgccg gaacgatggt cagcaatatg
    11941 gcaagaggtc caaatcgcat gtgccgcaaa aggcttcgcc tctgaccgcc ctcgacaagg
    12001 cgcgctggct cgccctcatc gtgcccgcgg cgctgctggc gggcgcgctc ggctcgcaat
    12061 atctcggcgg gctccacccg tgcgagatgt gccactggca gcgctggccg cattatgccg
    12121 ccgtggcgct ggcgctggtc gccttcgcca tacgcggcgc cgccgaccgc ggccgcagct
    12181 tcgtgtggct ggcggcgctc gccatcctca ccagcggcgc gatcggcgtc taccatgccg
    12241 gggtcgaggc gggcatcttc gaaggcctca cccaatgcac cgccagcgcc gccggcggct
    12301 cgtcggcgga catattggcg gatattatgg ccaacccgct cgttcgttgc gatcaggtgc
    12361 aattttcgtt cctcggcgtc tcgatggccg gctggaacgc gatcgtgtcg atcggatcgg
    12421 cattggtgat cctatggctg agcctcaaac gaccccgagt ggccgccgca tgaccgaccg
    12481 gcgctccgac tggcgcccgg gcgacgaccg cgccgacgtg gcttcgatga tccgcgtcga
    12541 ccaggccggc gaatatggcg ccacccgcat ctatgcgggc cagctcgccg tgctcggcaa
    12601 ccgccacccc gccgcccgcg tcatcgcccg catggcctcg caggaggagc ggcacctcaa
    12661 ccatttcgac caattgctcg ccgaccgcgg catccgtccc accatcctgc agccgttctg
    12721 gaacgtcgcc ggccacgcgc tcggcgccgt caccgccttg atgggcccgg acgcggcgat
    12781 ggcctgcacc gccgcggtcg aaaccgagat cgacaagcat tatgccgatc agctccgcgc
    12841 gctcggcgac caggatccgg acctttccgc caccatcacc gagttccagg ccgaggagct
    12901 cgagcatcgc gacaccgcgc tcgccgccgg cgccgaaaac acgttcggct atcccgtgct
    12961 ttccgctttc atccgcgccg gctgccgcgt agcgatcgaa ctttccaaga ggatctgagc
    13021 catgagatac acgctcctcg ccgccttgct gctggccggc cccgccgccg cgcaggaggc
    13081 cgcccctgcc gccgggccga ccgacgccaa ggtcagccag ctgatcgtct atggcgacga
    13141 cccctgcccg caaagcagcg ccgacgagat cgtggtctgc gcgcgcaagc ccgaaggcga
    13201 tcgcttccgc atccccgagg agctgcgcgc caaccccaac gaccccgcca accagagctg
    13261 gaccaaccgc gcgagcgagc ttcaatatgt cggccgcgcc ggcatcggca gctgcacccc
    13321 ggtcggcccc ggcggcggca ccggctgcta tgccgagttg gtccgccagg cgcgggccga
    13381 gcgcgccggc cgcgaatcgg tcaattggaa ccggctggtc gagcaggccc gccaggaacg
    13441 gctcggccgg atcgacgccg aagccgaggc cgaggaagcg gaagcggtcg cgcggggcga
    13501 atagccgcag cgccgccttg gaccgcggcg gcggcgcccg taatatggaa caaatacaag
    13561 gcttcggtcc ttctctcctt catctcctga agggaggccg aaggtgaaat ttgtcctcgc
    13621 tgccccgttg ctcgccgccg tcgcggccag tgcgctttcc gcccaaagcc cggccgacca
    13681 gcaacggcgc caagtccgcc aggtgatcgt ctacggcacc gatccgtgcc cgcgcagcac
    13741 cggcgacgaa atcatcgtct gctcccgccg ccccgagacc gagcgctacc gcattcccga
    13801 gggcgttcgc gacgagacgg acgacgacga tcccgacaac gaaagctggg ccgagcgcgc
    13861 ccgcagcctc gacgagccag cccgcgcgat gaccggcccc ggcgcctgcg agccggtcgg
    13921 ccccggccat cataccggct gcaccaccga aatgctcaac gcccaccgcg ccgcccgcga
    13981 agccgaggaa gcggaagggc cggagcgctg aggagggggg gtgattgaag tcactcagga
    14041 cttgatcatt atcgctacgc cggagttgac catcatcgtc accccggact tgatcccccc
    14101 cgtcaccccg gacttgatcc ggggtcccgc ttcttcttct cgctgccttg gagcgaagaa
    14161 gcgggatgcc ggatcaagtc cggcatgacg agcctactga cgtagcctca cgcccgctgc
    14221 tccgccgcga tccactcgtc gacctgggct tcgagcacgt cgagcggaac cgccccctgc
    14281 cccagcacaa catcgtggaa ttcgcgcagg tcgaacttcg cgcccagcgc cttttccgcc
    14341 cgcgcccgca gctcacggat cttgagctcg ccgatcttgt aggccagcgc ctggccgggg
    14401 tcggaaatgt agcggttcac ctccgcctcg atattggcct ccgatagggc ggtattgtcc
    14461 ttcatgtagc cgaccgcgcg ctccttgctc cagcccttgg aatgcatgcc ggtatcgacc
    14521 accagccgcg tcgcccgcca catctggtag gacagcttgc ccatctcctt ggccggcgtg
    14581 tcgtaaaggc ccatctcctc gcccaggctc tccgaataga gcccccagcc ttcgacgaag
    14641 ccggtgaaga aggcgagata tttgcgcatg ttgggaaggt cgagctcctg ctgcagcgcg
    14701 atctgctgat ggtgcccggg caccgcctca tggacgctca gcgccggtat ctcccacagc
    14761 ggcctttggt cgagcttcga ggtgttgacg taataagtgc cggcgatgcc gttctcgagc
    14821 gatccgaaat tgtaataagc cgtcgtcgtg ccttcggcgg tctcggccgg gatggcgcgg
    14881 atgccgtaag ggagccgcgg cagctttccg aacaggctcg gcatcttgcc gtcgatctgc
    14941 ttcagcacat aggccgtctc gcgcatcagc tcgtcgggcg tcttggcata atatttgggg
    15001 ttggtgcgaa ggtcggcgat ataggcctcg cgggtcgcga agcccgcctt cttcgccacc
    15061 gcctccatct cggcccggat ccgcgccact tccttgaggc cgatcgcgtg gatctcgtcg
    15121 gccttaaggc cggtggtggt catctgccgg atgcggaaat tgtaataatc gcgcccaccc
    15181 ggctgcgccg acacgcccac cgccttggcg catttcggcg ccacctcgct ggtgtagaaa
    15241 gcgagatgct tgcgataggc ggggtagagc gtggcctcga tcagggtccg cgcccgcccc
    15301 tgcatcgccg cccagtcggc ggcgctgacg tccgtgggct tggcgcgcgt gaacggctcg
    15361 tagaagcgcg acttgtcggg cgcgccggcg atcgtccctg agatggtctt gtcgtgcccg
    15421 gccatggcgg cgcagggcag cacataaccc tccttcaccg cccgcgcggt gatgctgagc
    15481 gcctcgtcgt tgatcttcgg atattgttcg agccgcttca gatagctgtc gtaatcggcc
    15541 gcggtccgga acggcagcac gctcgccatg tccgccgtcg actgatgcca gccgccgctg
    15601 ttgctgtaga ggatcatgcg ctgcccgtgc cggttggcct cgatctgctc ttccagcatc
    15661 cgcttcaata tcgcccggtt ggtgcggtcg gccgcgctca gcccggctgc cgggatcgcc
    15721 tccagccgcg ccagcaaagc ctgcgcctcg cgcgtccgac ggtcggccgc tgcgaggctg
    15781 acgtcgggca cgcgcccctc gtggtcgcgc acgccgacga gggtcgcgaa caccggattt
    15841 tcccgcatcg tcgaggccca atgctcgtcc agcagtcgct tgagatcgtc cgccggcgcc
    15901 gccgcggccg gcgcgctccc ggccagcaag agagcgatca atatcccgcg cattccattt
    15961 cctcctgtcg agcgccggcg cggatcggca cctgagccca agccccaaac cgttcgggct
    16021 gagcctgtcg aagccctctc ctccccttcc gtaccggacg agaaggcgaa cggagtgcga
    16081 gtcccaccgg tcaccctcac gacctccccc gctccgcggc gagccattcg tcgacctgcg
    16141 cctcgagtac gtcgagcggc accgaaccct gcagcagcac cgcgtcgtgg aagcgccgga
    16201 ggtcgaaccg ggcgcccaat gccttttcag ctttggcgcg cagctcgcgg atgcggatgt
    16261 tgccgatcat gtagcccagc gcctggcccg gccagctgat atagcggttg acctcggcat
    16321 cgatattcgc ctccgacagc gcgctattct cgcgcatgaa ggcgaccgcg cgcgccttgt
    16381 cccagccctt ggcgtggatg ccggtatcga ccaccagccg cgccgcccgc cacatctggt
    16441 aagaaagctg gcccatcttc ttggccggcg tgtcgtaaag gcccatcgcc gcgcccaggc
    16501 tctcggcata aaggccccag ccttcggtga aggcggtgaa gctggtgaag ttgcggcgga
    16561 acggcgcgat ctccagctcc tgggcgagcg atatctggtg atgatgcccc ggcaccgcct
    16621 cgtgcaggct cagcgccggt atctcccaca gcgggcgctg gtcgagcttc gatgtgttca
    16681 cataataagt gccggcaatg ccgctctccg gcgatcccga gccgtaataa gccgtcgtcg
    16741 tgccctcggc gatctcggcc ggtatctcgc gaatgccgta ggggagccgc ggcagcgtct
    16801 tgaagaaggc cggcatcttg ccgtcgatcg ccttggcgac gcgcgcgctc gcctccatca
    16861 gctcttcggg cgtcctggca taatattgcg ggttggtgcg cagctcccgg atgaaggctt
    16921 cgcggctcgc gaaccccgcc tgcttcgcca ccgcctccat ctcggcccgg atgcgcttgg
    16981 cttcggcgag cccgatcgta tggatctggt cgggcgtgag gtcggtggtg gtctcctgcc
    17041 gcacccggaa ggcgtaatat tcgcgcccgc ccggctgcgc cgaaatgctg tcggccttgg
    17101 cgcagcgcgg cgcatattgg ctgcggtaga aagcggcatg tttggcataa gccgggttca
    17161 gcacctcagc gatgatccgc cgcgcccgcg cctgcatctg cgtccattcg gccgcgctca
    17221 cgtcgaccgg ccgctcgccc gcgaacggct cgaagaagcg cgacttggcg gcatcctcgg
    17281 cgataacccc ggcgatcccc ttctcgtaat tgccgagcac cgagcagggc agcacatagc
    17341 cgccgcgcac cgcctcggcg gtgaccgcca gcgcctggtc gttgagcttc ggatattgtc
    17401 cgagccgggt gaggtagctt tcgaaatccg ccttggtgcg gaacggcagc ccattagcca
    17461 tgccggcaaa gccctggtgc cagccataat aggtcgtgaa cagcatcgtg cgctggccgt
    17521 atcggttggc ctcgatctgc tcttccagca tccgcttcag gatcgcgcgg ttggtccggt
    17581 cggccgcgct cagcccggcc gccggaatcg cgttcagccg ggccaggaag ccccgcgcct
    17641 cctccccccg ccggtcgcgg gccgccgggc tgtaatcgtc gatccgcgca tcatggtcac
    17701 gcacgcccag cgccgtcgcc tgcaccgggc tctcgcgcag cagccaggca taatggtcct
    17761 cgagcagctg cccgaaatcg ctggccggcg cggccgcggc cggggcggcc agcgccaaag
    17821 cggcggcaag gacgaaggat cggcgcatcg gtcactcctg gaacaggcgc gctcacccta
    17881 tccgcccctc gccgccgcgc aagggcggct gaaacgatcc gcgcgcaggc tggttgtcat
    17941 gccgaggccc aagcgagcgg aggcgatatg aacgacaatg tctacaagat catcgaggtg
    18001 gtcggcagct ccgccaccgg catcgaggac gccatcgaaa aggccatcgc ccgcgcctcc
    18061 gcctcgctcc acgacatacg ctggttcgaa gtcaaggaaa cccgcggcca cgtcgaaaac
    18121 ggccgcgtcg cccactacca ggtgaccctc cgcatcggct tcaccttgcg ggactgagcg
    18181 cctgaccgtg cacgagtcct tcgggacctt cccgctcatc ctgaggagcc gctgagcttg
    18241 tcgaagcggc gtctcgaagg acgcacggtt ccggtccttc gagacgggcc ttcgccgggg
    18301 ctcagtccct cctcaggatg agcggtttac ggtctccccc tcacgctccg ccggcccgcg
    18361 ccccgcaaca gcaccccctc ccccgaaaac cgctggcatc ctccgcattt gccgataggc
    18421 tgcggccccg gaacgaacgg gaggatgcgc gcatgacctt ggccgaagcg acgccggagc
    18481 tcgacatcgc tcgcaacgac cgcaacgacc gcaacgaccg cgccttcctc ggccacccca
    18541 agggcctcgg ctacctcgcc ttcaccgaga cctgggagcg cttctcttat tacggcatgc
    18601 agaccctgct cgtcctctac atggtcaacg agctgctgct ccccggccat gtcgagaata
    18661 tcgccggctt cgaggccttc cgcagcctct acggcgatat cgacggccag aagctcgcct
    18721 cggccatctt cggcacctac acctccttgg tctatctgac gccgatcctc ggcggcctgc
    18781 tcgccgaccg cgtgctcggc aagcgccgca ccgtcatgct cggcgcgatc accatggccg
    18841 ccggccactt cctgatggcg ttcgagacca gcttcctgct cgccttgctc tgcctcatcc
    18901 tcggcaacgg catgttcaag ggcaatatcg ccagccaggt cggcgcgctc tacggccccg
    18961 acgatctgcg ccgcgccacc gccttccaga tcttcttcct cggcatcaac gccgccgtca
    19021 tcgtctcgcc gctggtggtg gggacgctcg gcgagcaggt cggctggcat tggggcttcg
    19081 gcgcggccgg cgtcggcatg ctgatcggcc tcggcgtcta cattgccggg cagaagcatc
    19141 tgcccgagga gcatttcgac cgccgcgccg gcgccggcgc ccgcgccgag cgcccccgcc
    19201 tcaccgcgac cgactggagc gccctcatcg cgctcttcct cctgatcccg gtgatggcga
    19261 tcggcttcct ccccaaccag cagatcttca acgcctatct ggtctggggc gaccgccagt
    19321 tcgacctcac cttcatgggc acccggctgc cgaccagctg gctgatcacc ctcgacgcga
    19381 tcaccagcgt cagcttcctc gccatcgtcg ctttgttcta cagctggtac ggcaagcgcc
    19441 gccgcgagcc cgacgaagtg accaagatga tcatcggctc ggtcttctcg atcggcggca
    19501 tgctgtgcct gttcatggcc gcctcgacga cggccgaggg cgagaagatc ggcctgttct
    19561 ggccggtcat gttccatctg ttgaacagca tcggcttcgc ccacatgctg cccgtcagcc
    19621 tcgctctgtt cgccaaggtc gcgcccaagg ccgtgaacgc caccgtgatc ggcatctatt
    19681 atctcacctt cgtcgccgcc aacgggctgg tcggctatgt cggcggctgg ctcgagacga
    19741 tgccggccac caccttctgg ctgatgcacg ccggcttcgc cgccgcggcg ggggcggtgt
    19801 tcatcctgtt caagctgttc ctgtcgaagc gcctcgtcca gtccgaaccc gccccgctgc
    19861 ccgactgagc cgccaagtcg aaccggccat aagacgaggc tcgaccagtc gcggccgccg
    19921 cacgcagccc cgtttaaccc gcttgccaag acgtgtccca gttcagggcg gtcgccttga
    19981 cgccccagaa agcgttttga gagacggcta aagtcaagat atcgttgttc ttctagcgct
    20041 gacccgatcg gccgcgtccg ggcagtgacg acaaggggaa tttgcgatgc agaacttcca
    20101 ggtgcgctca cccgactccg tcccgtctcc gcccaaggcc gacagcgccg ccgacaaggc
    20161 cgaacggccg caaatgtcga tgatcggcgc cgacatcatc atcaccggca atatcgaggc
    20221 ttcggtcgat ctccacatcg aaggccgggt gatgggcgac gtccgctgct ccaccctgat
    20281 cctcggcgaa agcagcatca tcaacggcgg catccatgcg gaccgcgtcc gcgtctcggg
    20341 catggtcgaa ggcgccatcg tcacccgcga cctcgccatc gaagccaccg cccgcgtcac
    20401 cggcgaagtc tcctacgaaa ggctccgcat cgccaacggc gcgatcgtcg acggcacgct
    20461 gaagcgcaac gccccgcccg aagccagcgc cgacgccagg ctcaagctgg tcgaggctaa
    20521 ggccgaagcg aagctccagc cgaagctcga ggccgagccg gcggtgatcg tcatcgaatg
    20581 acgatctggg gcgtcgcggc tacggaagag gcggacatgc tttcccagca gatgaccata
    20641 gtcgtggtcc cgcccgagcc ggacctcact ccggcgaccg ccttcgagga attggaggcg
    20701 atcctctaca ccgtcgccgc ctcgccgccc cagcaccgca agttcgcggt ccgctacatg
    20761 caatgccgcg acgtgctgat ggggagcgag ctccgcgcca ccctgcccgg cttcatgctg
    20821 cagaccggca cgatcgacaa atttcgcgac ttcatcacgc tctacgaccg cgacgtgccc
    20881 gcgcgccgcg cctttctcga tcatgcgctc ggccgctgcc gcgcgctggt cggcccccag
    20941 cgggtctatg acgatttcgc cgacctcgat ttttgagatg cgcctctggt gcgcgctcgc
    21001 cggccacgcg cccgacgccg ggctggcgac gtggaacctc ggctattgct tcagccgctg
    21061 cgaacgctgc ggccgcgaca tcgtccgcac cggcgagggc cgctggaagg tcccgcacgg
    21121 atatcgcatc gtctggcagc cgctgccggc caaggctgcc gatccgcccg agcccgccga
    21181 agccgagctt ccgcccgcgg ccgagtcgga gccgctcccc tggggagagg tcgccgaccc
    21241 ggtccatttc gacgccgctc ccgaggccga gccgctccta accgaaccgg agccactgcc
    21301 cccggaaaag gtcgcggcgg aacccgagcc gatccccttc gaggccgcgc cggaaatcgc
    21361 cccgtccgag gatgagccgc ccgcgccggc gatcgagccc ggtccgcctg ccccggtgga
    21421 ggacgagctg cccttgccac ctgctgggat cgaggaggaa gaggcgcctt tgccgcaagc
    21481 cgcgatcgag gttgaggcgc cgctgccgca acccgaacgc gcccgcgact tcatggacat
    21541 ggacgtggag gaggaggatt gggacgatct cgccgccctg ccgcctgccg caatggcggc
    21601 cgccgccccc gcggccgccg cgccgcgctg gctcgctttg cccgactgaa cctttattcg
    21661 gccgccagcg gctccggcgc cgggcgccgc ctgatccgag ccagcagccg cggcagcggc
    21721 gcgttgcgct ccatccaggc ggcatagtcg cgataggccg gatcggccat caaatgccgc
    21781 tcctcggtcc gcgcccgcca ataatagacc ccgctcacga ggccgagcac gaccgtgttg
    21841 cgcaccgcgt cgaccgggtt cccgctcgcc gccaggaacg gcatcgtcgc cagccaccag
    21901 aacagattct tggagacata ggccggatgc ttggtccagg cgtacgggcc gtgggtgagg
    21961 atgccgcggt gggtgaggtt ggaaaagcgg atgccgaacg ccaccgtcgc ccaggcgtaa
    22021 taagctgtga gcagcaccag cacggccccg atgacgccga gcgccagcgg cgaccctgcg
    22081 aaccagtgcg tccattcgcc cgcgccctgc tgatagttga gcggcccgcc cgggttcatc
    22141 aggatgaacg gcgggtagca gatcagcgcg gcggtccagc cggcggcata ggggttggcg
    22201 gagcggatat gggcgtcgag cggcttcatc gtcagcatgt agccgaccgt cgcaaaggcg
    22261 acgtcgatca ggaacatcgc gccgaccagc cagtgggtga gggtcaccgg gctcgcgagg
    22321 atgtccgaca gcgtgggccg gaccgtgtcg gtccagttgc ccggcacgat cgagatcatg
    22381 aaggcgagga agaagccctt caccgcccag gcgcggaaga aatcgtaaag cagctcgcgg
    22441 tccagtccgt cggtgcggcc gatgatcagc cgtccgaaat gccaggcgcc gtcgcgcggc
    22501 tctttcagcc gccggtcgag ccacaccaca tagggcaccg acaggatcag catcggcagc
    22561 gcggccaccg caagcgcctg catcgcgaac agatattggc cgtcccaata ccagcggccg
    22621 acgcaataga ggaaggcgat cgccgcccag atgccccaca ggccgacgat cttggccagg
    22681 ctgatgtcgg cgctctcgcg cagcggccgc ggcggcgagt cccagtcgat cccggtcgag
    22741 gggctgcggt ggaccttgtc gaccagcagc gaccacaaca ccatcggcag cccgcaggcg
    22801 acgagcgcga ccatcgcata gcccggcccg tcgaggccgt agctgccggc gaatatcgcc
    22861 cagccgccga ggccggcgag gccagcgacg ccgacgccga cgctcaccgc cgatcgcggc
    22921 cgcgggtcgg cggcggtcac ggcatcgatc ctggataaag cgttcatcgc ctcgcattaa
    22981 ccgacaagtg gtaagcaacc ggtgaaggcg gccgcgcgcc ccccgccccc gcccctcgcc
    23041 ccctcgtcgg gcgatgccgt agcttcgcgg gtgggcgcgt aaggcttctt aaacgcttgg
    23101 gcccctattt taggctttgc ggcgagggac atcgggggat gctgcgcggc cgccgcggga
    23161 gggatggtgc gatgaatctg gcctatgagg agaccacctt ctccttctcg agcgaggcgc
    23221 ccggaccgca ggagcgccgc cgcgataccc ggcacatgac catcttgcgc gtcggcaccc
    23281 tgatcgtcga cggcgcccgc gagctctgcc tcatccgcaa catctcggcg ggcgggctga
    23341 tggcccacgt ctattcgcag ctcgacctcg gcaaggcggt cgccgtcgag ctcaagacca
    23401 accagcagat cgccggccgc gtctcctgga tcaagggcgg caatatcggc atcgccttcg
    23461 atcagatcat cgacgtcgag gaattgctcg ccagccagaa cgcgctcgac ggcggctggc
    23521 ggccgcggct gccgcgggtc gaggtcgacc gcatgggcac gctccgcgcc ggcgcccgca
    23581 cccactgggt tagcacccgc gacatttcgc agggcggcgc caagctcgac accgatcagc
    23641 cgctcgatcc cggcaccgac atcgtcctca cgctcgaagg cttccgcccg ctcgccggcg
    23701 tcgtgcgctg gtcgcatgag gggctgtgcg gcgtctcgtt caaccaggtc attccgttcg
    23761 gcgaactgat gggctggctc cggcaggcct gaggccgcta cttcctgccc tcggttcccg
    23821 ctccaccggg acggcgccgc ccgatccacc aggcgtggcg cccgaccacc aggtccggca
    23881 cctttttgcc ctcggcgtcc agcgccggcg cgtagcggaa acgccgctcg atcagccggc
    23941 aagtggtggc atcgagctcg gcatggccgc tcgaccgcgt gacgatgcag tcgctgaccc
    24001 cgccgtccgc cgcgaccttg aagctcagga agacgacgcc ctcggcccgc ttccgccgcg
    24061 ccgcccgcgg ataatccgaa tcgagcaacg ccccgccgat cagccgcgcg ctccgcgcca
    24121 ccccgccgcc gccgccgctt ccggtgctgc tcccggcgcc gccgctgccc gtccccacgc
    24181 cgctcccgct cgaacttccg gccaagacgc ccacgcccga tcccaccacc ggcgcggcgg
    24241 ggagcggcgg cgcagcctcg acccgcacct ccggcggcgg cgccagcacc ggcgacgcgg
    24301 ccggggcggc cggggcggcc gcggcttctg tcgccttctc aagcttggga gcaggagcgg
    24361 ccttcacctc tggcggcggc ggcgcctcgc gcacctcgaa caatttcagc cgctcctcgg
    24421 cctgccgcga cacctcgacg ccgaggccgc cgatcagcgc gtaaccgagc aacaggtgaa
    24481 acgcggcggc ggcgcccgcc gacgtcatcc gctcccgctt ccgcccctcg cccatgtccg
    24541 cattatcgca ccgaagcgcg gcaccggcca gatcaaacct gctcgctaaa ccccgtagcg
    24601 cccccctcaa tcctgccgct tgcccgtctt cttcgccggc gcattctcgt tgccgccggt
    24661 ctcctgctcg ccgagctggc cgccgccatg atagggcatc tcggtctggc cgccatggcc
    24721 catgaagccg ccattcttgg ccgacttgcc ggtatgcggg ttgggatagg cgccgccgcc
    24781 cgaatcgccg ccgccgagcc cgctctcgcg cccgttgaac ttgtccatcg cctccgggtc
    24841 gccgcgggcg aggtcgccgg ccgcgccgtg cgagcctgga ttgtccggcg aatgggcgtc
    24901 gcgattgggt ccgagccgtt tgatcatcgt gctgcctccg tgccccccga acgcgccgcc
    24961 cgccgctccg ttcaatcctc gaggatcagg ctgtgctcgc gcgtgtcgcg catccgccaa
    25021 tagacgatca gcgacagccc gatcatcccc gtcacatacc agtagaaagc ccgctccacc
    25081 cccgcatcct tgaaccacaa agcggcatat tcggcggtgc cgccgaacaa tgtgttggcc
    25141 agcgcataag gcaaggcgac gccgagcgcg cgggtgtggg cgggaaacaa ttccgccttc
    25201 accaccgcgt tgatcgacgt gtagccggtg acgatcagca gcgcgccgaa caccaaaaga
    25261 aaggcgagaa acgggtcgcg caccgtctcc agcgtgcgga agatcggata ggtgaggaac
    25321 aggccgagca gcccgaagcc gatcatcagc ggccgccgcc cgacccggtc cgacagggcc
    25381 cccgccgccg gctgcagcag catgaacagg aacagggccg cggcggtgac ctgcgtcgcc
    25441 gtctggcggc tgaagcccga cgtgttgacc aggaatttct gcatgtaggt cgtgtaggcg
    25501 tagaaggcga gcgtgccgcc cgcggtcagc gccatcacca gcagcgcctc cctcggatgg
    25561 tcgcggaaca ggccccagcc gctcgccttg ggcgcgccgc cgtcgcgcgc attctcgaag
    25621 ctttcggtct cggtcagccg ccgccggatc cagaacacca ccaccgccag cgccgccccg
    25681 atcgcaaacg ggatccgcca gccccacgcc tccagcgccg cctcgctcat caccgcctgc
    25741 agcaggatca gcacggcgag cgcgatcagc tggcccgaga tcaaagtcac atattggaag
    25801 ctcgagaaga agccgcgccg ctcccgcccc gccatctcgc tcaaataggt ggcgctggcg
    25861 ccatattcgc cgccgacgct gagcccctgc agcatccgcg ccgccaccaa tatcgccggc
    25921 gcccaggcgc cgatcgcggc atgagtgggc gtcaccgcga tcagcagcga cccgccgcac
    25981 atcaacgcca ccgacagcgt cagccccgcc ttgcgccctt tgcggtcggc atagatgccc
    26041 atcgcccagg cgccgatcgg ccgcatcagg aagccgaccg cgaacaccgc cgcggcgctc
    26101 agcaattcgg cggtgcggtc gccagccggg aagaagaccg gcgcgaaata gagggtgaag
    26161 gccgaataga cgtaccagtc ataccattcg acgaggttgc cggccgatcc gccgataatg
    26221 ttgcgcagcc ggtgcgcgcg gtcgaagcgg gcggtttcca ccatcagcgg accaattcgc
    26281 cccccttcat caccgcccgc acgcactgcg tcgccgcctc cgccccgccg gcaaacagca
    26341 aagccgcggc gacggcagcg atcccccgcg cgatcactcc ccctcctgct gctggcggcg
    26401 cttgatattc tgggggaagc agccggtata gccccccggc ccgaccgtgg agcaggagcc
    26461 gatcccggca tcgccttcct cgatcagcct gtggcgctcc cgcgacacgc tgtccgtcgc
    26521 gccgccgggg tcgaaccggt tctcgcggat cggcagccgg tacttctcgt tgtcgcgccg
    26581 tctgcagaca acgatctcat cgtcttgccc ttcgccgacg gggccgcaac cgcgctccac
    26641 tcggaaggtc tcggccgcga ccgccatata gtcgccgctc tcctcctcgg gcggcgcggc
    26701 cgtggccggc gccgagcaca gccacaccaa aatcacggcg ggccgcgccg gcagccgcgc
    26761 cagcaccggc cgcgcctcag ccatgcgccg cagccgaacg ccggctgccg tagaacaggg
    26821 agatctgggt ctggtgcggc aaagctccct cgaggtagga cagtcgccga gcccgcacgc
    26881 gcctgggctt gtcgaaagcc tgtgcgtcag gagcagggga gagcgtcgcc cgcgctacaa
    26941 tcaccgaact cacgcagcgg gttccccata gccatgctcc tgcaatcgtg cgcggcacat
    27001 gggattctga atcgccttga aggccgcgcg acgttgttct tccgtggcct cactacacgg
    27061 caagccgggt tcccgaacgc ggcgatcgca ccagccgcaa acctgtcctt ccatttcctg
    27121 tggcgtagga gaggcagcaa agcgatccgc ttgcggcgcc tccggttgct tttggagttc
    27181 ccctggcaaa aaccacaacg ctcgaacgat cgatccaacc gcccataaca ccagccacag
    27241 ctggaagagc gcaccggcga ggacccaaag tgcgaattga agcgctactt tgccggggtc
    27301 gatgcccgaa taggtcgtca catccatcga gtacgcgacc agcgcgaaga gcagcgaggc
    27361 cgcaaataaa atccacgcaa tggcgaggga ggtgctgccg tcggggcggc taatcatttt
    27421 cccggactcc cttcttacgg ggttcatcga ctataatccc aggtccggca gcccccgcag
    27481 ccgagcgccg gctgccgtag agcatgtaga aaaccagccc gatgacgttc cagatcagga
    27541 aatagatctg ggtcgtctgc ggcaggctga ggaacagata gatgcagccg aggatcgcga
    27601 ccagcccgac cggccaggcc aaaggc
//
LOCUS       DASVRK010000039         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_441624, whole genome shotgun sequence.
ACCESSION   DASVRK010000039 DASVRK010000000
VERSION     DASVRK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441624"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VF589_01525"
     CDS             complement(<1..619)
                     /locus_tag="VF589_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256286.1"
                     /translation="MAKAKTGGAKSGGDKKTGGRKSGASRSGGSARKSSASSGSGGGS
                     SGSGGGGENRVSAALRDAGQRAAELAQNPVARNMLAAGLVAAAAALTSSSRIRESAKQ
                     AGRDAADNAEAAADSATRIGAAIVTAATDAVRRVLGGGSDDDGESGQTLASAPARKTR
                     AASGTKTGGRAKSAGTGAAKRAGTGRAASAKAGGAASGATASTGGR"
     gene            complement(713..1183)
                     /locus_tag="VF589_01530"
     CDS             complement(713..1183)
                     /locus_tag="VF589_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256287.1"
                     /translation="MMEQDRDGMSGSELQESQSGGESGEFGGNRAQQQSGQGGIGGQQ
                     IGGGAEAPDMGAGGGSSGTGGYGNAQNQANHQGQQGGAGQAAYGNSGRGAAFDEAQGG
                     GRGVDDISRSEDERYGGSSGEFDEEDGNPAADEFLRDQQAHQDRGQSEAEEEAG"
     gene            complement(1205..1417)
                     /locus_tag="VF589_01535"
     CDS             complement(1205..1417)
                     /locus_tag="VF589_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256288.1"
                     /translation="MGSSDISGGKGHPLPGSDEARHKEIPEDLEEAKGRGDDPAPVAT
                     PNPTGVNPDGEPYRYDDLGRGENISD"
     gene            complement(1419..1649)
                     /locus_tag="VF589_01540"
     CDS             complement(1419..1649)
                     /locus_tag="VF589_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256289.1"
                     /translation="MQEREREREETERGQPASFNPVSGEVHGSGSGAGGGGNPNEDYD
                     SDPMAGSGSEPAGGPRPRSDAVDRPIDPDEGV"
BASE COUNT          195 a          745 c          622 g          349 t
ORIGIN      
        1 cgcggccccc tgtgctggcc gtggcgccgc ttgccgcgcc gccggccttg gcggatgccg
       61 cccgaccggt gcccgcacgc ttggccgcgc ccgtgcctgc gctcttggcg cggccgccgg
      121 tcttcgtccc ggaggcggcg cgggtcttgc gtgccggagc cgaagcgagc gtctgaccgc
      181 tttcgccgtc gtcgtccgag ccgccgccca gcacgcgccg caccgcgtcg gtggcggcgg
      241 tgacgatagc ggcgccgatc cgggtcgcgc tgtcggccgc ggcctcggca ttgtcggcgg
      301 catcgcggcc ggcctgcttg gcgctctcgc gtatcctcga gctcgacgtc aaagcggccg
      361 ccgccgcgac caaaccggcc gcgagcatgt tgcgggcgac cgggttctgg gcgagctcgg
      421 cggcccgctg gccggcatca cgaagcgcgg ccgagacgcg gttctcaccg ccgccgccgc
      481 tgccgctgct gccgccgccc gacccgctcg aggcgctgct cttcctggcg ctgccgccgc
      541 ttctggacgc gccgctcttc cggccgccgg tcttcttgtc gccgccgctc ttggcaccac
      601 ccgtttttgc ctttgccatt gcagtctcct ggctttttgt aactgacgcg taacagacgt
      661 gcggacgggg caacggttcc ttgccccgcc gcccgctacc ggcgaatgat atttagcccg
      721 cttcttcctc ggcctcgctc tgcccgcgat cctggtgcgc ctgctggtcg cgcaggaact
      781 cgtcggcggc gggattgccg tcctcctcgt caaactcgcc ggagctgccg ccgtagcgct
      841 cgtcctccga gcgactgatg tcgtcgacgc cgcgcccgcc gccctgcgcc tcgtcgaacg
      901 cggcgccgcg gccgctattg ccgtaggccg cctgccccgc cccgccctgc tggccctggt
      961 ggttggcctg gttctgggca ttgccatagc cgccggtgcc gctgctgccg ccgccggcgc
     1021 ccatgtcggg cgcttcggcg ccgccgccga tctgctggcc gccaatgccg ccctgcccgc
     1081 tctgctgctg ggcgcggttg ccgccgaact cgccgctctc gccgcccgat tggctctcct
     1141 gcagctcgct gccgctcatc ccgtcgcgat cctgttccat catcatgggt ctccgttgtt
     1201 gcgcttaatc tgagatattc tcgccgcggc cgagatcgtc gtagcgatag ggctcgccgt
     1261 cgggattgac gccggtgggg ttgggagtgg cgaccggcgc gggatcgtcg ccgcggccct
     1321 tcgcctcctc cagatcttcc ggtatttcct tgtgccgcgc ctcgtcgctg ccgggcagcg
     1381 gatggccctt gcccccgctt atgtcagagc tccccatctc agacgccttc gtcgggatcg
     1441 atcgggcggt cgacggcgtc gctgcgcggc cgcggcccgc cggccggttc ggagcccgag
     1501 cccgccatcg gatcgctgtc ataatcctcg ttgggattgc cgccgccccc ggcgcccgat
     1561 ccgctgccat gaacctcgcc cgacaccggg ttgaacgagg ccggttggcc gcgttcggtc
     1621 tcctcgcgct cccgctcacg ctcctgcatc cgcgcctcct cgataggtgc ggggtgaacg
     1681 cgccgccgcg cgctgccgtt ccgccgccgc atcgaacggg ccagcggccg cacccgttta
     1741 tcgcagccca tggcacccat cgcgccggcg atgcgttacg tagccggacc aaccccaatg
     1801 aggagcaaag tatgtttgaa tcggataacc ggggctcggc cggcgccagc catgacatca
     1861 gtgtcctcaa cagcctgatc gagaccacca tcgacagcgt cgacggctat c
//
LOCUS       DASVRK010000040         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_379541, whole genome shotgun sequence.
ACCESSION   DASVRK010000040 DASVRK010000000
VERSION     DASVRK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379541"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VF589_01545"
     CDS             <1..934
                     /locus_tag="VF589_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8256290.1"
                     /translation="QRAGLILAPRIYSSGEIVYGARAPGAYAQIDNYNDALAHVRRLK
                     AQGAHSIKNYNQPRRDQRQQVVAAAKAENIAVVAEGASLFSQDITLIQDGNTTLEHNI
                     PQSMLYKDVLDFFGATRVGYTPTLGVTYGGPGGEPYWIQESQVWKHPLLTRHVPREQL
                     EEELTRVVSAPEEDYADQVSARTARLLSQRGVPVSIGGHGQQQGLAAHWDMWSFARGG
                     WTPIEALAAGTRTPARSLGFTDIGTLEPGKLADLVILDADPTQNIRNTERISKVMLNG
                     RIYDAMTLNEEVTGTRRRQPYHWETGGTAAAASQ"
     gene            1056..1376
                     /locus_tag="VF589_01550"
     CDS             1056..1376
                     /locus_tag="VF589_01550"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8256291.1"
                     /translation="MGMQDRNLALAARMITRTQERVVDQRSESRRDDVVGRATIVFRG
                     QEYLVPVVNISSRGTMVETEIVPRIGETIVVQFESCTRIHAFVRWVRDGRLGLNFGHE
                     IVLC"
     gene            1390..1716
                     /locus_tag="VF589_01555"
     CDS             1390..1716
                     /locus_tag="VF589_01555"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8256292.1"
                     /translation="MMLAQIALDHTEPRRREPRLDIAADIGLREFGTTGVEARLINLS
                     SRGFMAETRAIAKVGSRVWVTLPGGARANALVKWVRGGRLGGEFAAPIDPLPIFQAQG
                     KSKIGG"
     gene            complement(1864..2460)
                     /locus_tag="VF589_01560"
     CDS             complement(1864..2460)
                     /locus_tag="VF589_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX8256293.1"
                     /translation="MTLGMAAPAAAEDVRILAFGDSLTAGYGLPRGQGFAPQLQAALR
                     RNGVPAFVTDAGVSGDTAAAGRARLAWTLDSLDEKPHLAIVALGGNDMLRGLPPRQTR
                     AELEAIIAELKRREIPVLIAGMLAAPNLGANYGAEFNRIFPELAQKYQASLYPFFLAN
                     VAGIRGLNLPDGVHPNFQGIKRMVGGIVPEVLKALGTR"
     gene            2536..3225
                     /locus_tag="VF589_01565"
     CDS             2536..3225
                     /locus_tag="VF589_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8256294.1"
                     /translation="MNSPHIVIDASNVTLGLGSGAARVEILRGIGLQVASGESVALLG
                     PSGSGKSSLMAVLSGLERATSGTIHIAGADFTTLGEDDLARARRGRIGIVLQAFHLLP
                     TMTALENVAVPLELAGQEGAFDIAARELEAVGLAERLAHYPAQLSGGEQQRVAIARAT
                     APRPSLIFADEPTGNLDVKTGHAILDLLFARQQDTGATLLVITHDPALAARCGRVLEM
                     QDGRIARDSKA"
BASE COUNT          523 a         1198 c         1058 g          486 t
ORIGIN      
        1 gcagcgcgcc ggcctgatcc tcgcgccccg catctattcg tcgggcgaga tcgtctacgg
       61 cgcccgcgca cccggcgcct acgcccagat cgacaattac aacgacgcgc tcgcccacgt
      121 ccgccgcctg aaggcgcagg gcgcccactc gatcaagaat tacaaccagc cgcgccgcga
      181 ccagcgccag caggtcgtcg ccgccgccaa ggcggagaat atcgccgtgg tcgccgaagg
      241 cgcgtctttg ttcagccagg atatcaccct catccaggac ggcaacacca cgctcgagca
      301 caacatccct cagtcgatgc tctacaagga cgtgctcgat ttcttcggcg cgacccgggt
      361 cggctacacg cccacgctcg gcgtcactta tggcggcccg ggcggcgagc cttactggat
      421 ccaggagagc caggtctgga agcatcccct gctcacccgc cacgtgccgc gcgagcagct
      481 cgaggaggaa ctgacccgcg tggtcagcgc gccggaggag gattatgcag accaggtgag
      541 cgcccgcacc gcccgcttgc tgtcgcagcg cggcgtgccg gtctcgatcg gcggacacgg
      601 ccagcagcag ggcttggcgg cgcattggga catgtggtcg ttcgcccgcg gcggctggac
      661 cccgatcgag gcgctggcgg ccggcacccg cacccccgcc cgctccttgg gcttcaccga
      721 catcggcacg ctcgagccgg gcaagctcgc cgacctcgtc atcctcgatg ccgatcccac
      781 ccagaatatc cgcaacaccg aacgcatttc gaaggtgatg ctgaacggcc ggatctacga
      841 tgccatgacc ctcaacgagg aagtcaccgg cacccgccgc cgccagcctt atcattggga
      901 gacgggcggc acggcggcgg cggcgtcgca ataaggcggg gccgccgcgc cggctccccg
      961 gccgcggcgg ctccatcggt aaatgccggc cgatcgataa gaattcggta agcaaatccg
     1021 gtgcatgctc ctccttcgcg agcgggagag cggcgatggg gatgcaggac aggaatctgg
     1081 cgcttgccgc gaggatgatc acgcgcacgc aggaacgggt ggtcgatcag cgttcggaat
     1141 cgcgccgcga cgatgtcgtc ggccgcgcca cgatcgtctt ccgcggccag gaatatctgg
     1201 ttcccgtcgt caacatctcg tcgcgcggca ccatggtcga aaccgagatc gtcccgcgca
     1261 tcggcgaaac catcgtcgtc cagttcgaaa gctgcacccg catccacgct ttcgtccgct
     1321 gggtccgcga cggccggctc ggcctcaatt tcggccacga aatcgtgttg tgttgaaggc
     1381 gctccggata tgatgctcgc ccagatcgcg ctcgatcaca ccgaaccccg ccgccgcgag
     1441 ccgcgcctcg acatcgcggc cgacatcggc ctgcgcgaat tcggcacgac cggcgtcgaa
     1501 gcccggctca tcaacctgtc gagccgcggc ttcatggccg agacccgcgc gatcgcgaag
     1561 gtcgggtcgc gcgtctgggt cactttgccc ggcggcgccc gcgccaacgc gctcgtcaaa
     1621 tgggtccgcg gcggccgcct cggcggcgag ttcgccgccc cgatcgatcc gctccccatc
     1681 ttccaggctc agggcaagtc caagataggc ggctaattcc tcccccggct ttccggggga
     1741 gggggaccgc gcgcagcgcg gtggaggggc cacttcctcc cctgcctttg caggggagag
     1801 ggaccacgcg cagcgtggtg gaggggcaat cctcgcccaa acccgacccc ccaaacctcc
     1861 cgctcaccgc gtccccagcg ccttcagcac ctcgggcacg atcccgccca ccatccgctt
     1921 gatcccctga aaattggggt ggaccccgtc cggcaggttg agccccctga tgccggccac
     1981 gttggcgagg aagaagggat agaggctggc ctggtatttc tgcgcgagct cggggaagat
     2041 gcggttgaac tcggctccgt aattggcgcc gagattgggc gcggccagca tgccggcgat
     2101 cagcaccggg atctcgcgcc gcttcagctc ggcgataatc gcctccagct ccgcgcgcgt
     2161 ctggcgcggc ggcaggccgc gcagcatgtc gttgccgccg agcgcgacga tcgccaggtg
     2221 cggcttctcg tcgaggctgt cgagcgtcca ggcgagccgc gcccgcccgg ccgcggcggt
     2281 gtcgcccgac acgccggcgt cggtgacgaa ggcgggcacg ccgttccgcc gcagcgcggc
     2341 ctggagctgc ggcgcgaagc cctgcccgcg cggaagcccg tagccggcgg tcagactgtc
     2401 gccgaacgcc aggatccgca cgtcctcggc cgccgccggc gccgccattc cgagcgtcat
     2461 gagcagtgcc agatagacaa acaggcggcg cgccccatat gcgacccata acggcatcgg
     2521 aatatttctc ccttcatgaa ctcgcctcat atcgtcatcg acgcctccaa tgtcaccttg
     2581 gggctgggca gcggcgccgc ccgggtcgaa atcctgcgcg gcatcggcct tcaagtcgcg
     2641 agcggcgaaa gcgttgcctt gctcggtcct tcgggcagcg gaaaatcgtc tttgatggcg
     2701 gtgctgtcgg ggctggagcg cgccacctcc ggcacgatcc acatcgccgg cgccgatttc
     2761 accacgctgg gcgaggacga tctcgcccgc gcccgccgcg gccgcatcgg tatcgtgctc
     2821 caggccttcc acctgctccc gaccatgacc gcgctcgaga atgtcgccgt gccgctcgag
     2881 ctggcggggc aggagggcgc cttcgacatc gccgcgcgcg agctcgaagc ggtcggcctg
     2941 gctgagcggc tggcccatta tccggcccag ctctccggcg gcgagcagca gcgcgtcgcc
     3001 atcgcccgcg ccaccgcacc gcgcccgagc ctgatcttcg ccgacgagcc gaccggcaat
     3061 ctcgacgtca agaccggcca cgccatcctc gacctgctgt tcgcccgcca gcaggacacc
     3121 ggcgccaccc tcctcgtcat cactcacgac ccggccctgg cggcgcgctg cggacgcgtg
     3181 ctggagatgc aggacggccg catcgcgcgg gattcgaagg cgtgagggag gcatcacctt
     3241 gcctccctgc cgcgaagcgg cgggg
//
LOCUS       DASVRK010000041         7577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_131398, whole genome shotgun sequence.
ACCESSION   DASVRK010000041 DASVRK010000000
VERSION     DASVRK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131398"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..2694
                     /locus_tag="VF589_01570"
     CDS             16..2694
                     /locus_tag="VF589_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX8256295.1"
                     /translation="MSMRMLIDARHPEETRVAVAKGNRIEEFDFESAEHKQLKGNLYL
                     AKVTRVEPSLQAAFVDYGGNRHGFLAFSEIHPDYYQIPKEDREALLREEAEHAAEEER
                     LRAAEDARYDDDDDYAESSAERDSDDIEEVGDDAGGPSDDGHDDGHGDDEHGDDHGDD
                     GEGHEDEEGRAPPKPEAASGSEENAADELRRKRQNLRRRYKIQDVIRRRQVLLVQVVK
                     EERGNKGAALTTYLSLAGRYCVLMPNTSHGGGISRKISNGADRKRLKSIMTELNLPRT
                     MGCIVRTAGLSRTKTEIKRDFDYLARLWDEIRERTLGSSAPALIYGDSDLIKRAIRDI
                     YNRDIDEVLVEGEEGYRAARSFMKLLMPSHVRRVKSYADPVPLFQRFGVEDQLSGMYQ
                     PLVQLKSGGYLVINPTEALVSIDINSGRSTREHNIEQTAYATNIEAAHEIARQLRLRD
                     MAGLVVIDFIDMESNGHVRKVEKAMKEALKNDRARIQVGRISSFGLMEMSRQRLRTGV
                     LEASTKPCPHCEGSGLMRTASSAGLSALRMLEDEAARGRGSRIILRAGREAAVYVLNR
                     KRAEIGEIEARYGVIVEVLIEEALEGAKMSVESVGLPPLQRPRPVPIVEPLIDEEEDL
                     LEEEEEELDEEEEEAAEEPEAPREERRPPRPAAAAEDSESERGRKRRRRRGRRGGRRR
                     PGEEEGRREEGGDEPQVAGEDERIVAEEAAAEAPMAEAEPELEAPTDAGIAEAPAEEE
                     KPRKGRRRPRAREAEVSAPAAAPAEAPAAVEAAPIAEAAPAAEEAPAKPRRSRRKKAD
                     PEAEAPAEAPAPAVEEAAAPAPEETGAPAAANDTAEAAPAPKRRSRAKKVAEAPEAPE
                     APVLERVAADGPDADGGEAAESGPPRRGWWQRTFGA"
     gene            2926..4293
                     /locus_tag="VF589_01575"
     CDS             2926..4293
                     /locus_tag="VF589_01575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8256296.1"
                     /translation="MAQSLLRDAETEALFDEMARPLIEAAGMRPENVEVVLIGDKSIN
                     AFVAGGQIVYIHSGLITAAENANEVQGVIAHELGHITGGHIIRLGEGARVATGISILS
                     LLLGAAAMAAGAGNAGTAIMAAGQQAAMGKFLAFTRTQESSADQAGASYLRKAGITGK
                     GSLSFFKKLQNQEFRLAIPQQDSYARTHPLTGERLAALENVYTTDAAWQKPADTALEA
                     RFQRVKAKLAGYVDEPRQTLIKYPETDRTVPGRYARAYAYHKSAYPDRAAAEVDSLLR
                     DYPDDAYFLELKGQILLESGKPKEALESLRKSVRLAPNQPLIASTLGHALISTEDAKN
                     FAEAKQVLRAAVGRDNSNPFAWYQLGIVYDREGDPARAALATAERHSLEGNPLQAMAS
                     AQLAMAGVKPGTADCLRAQDIAMASRASILQNRRESTEESRVAKMIERNERRSGAPSM
                     ACGGA"
     gene            4290..5003
                     /locus_tag="VF589_01580"
     CDS             4290..5003
                     /locus_tag="VF589_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8256297.1"
                     /translation="MTGRNLAIAAGAAGLLAGAGAVLLARGGGDGLVGGERAQVEATV
                     RDYILANPEIIPEAMKRLQDRQLGKAVADNRQAFETPFGSAWAGAENADVVLVEFYDY
                     ACGYCRKTVSDLERLLAEDDGLKVVWRELPVLGPDSEAAAMVSLAAARQGKFKAFHNR
                     LFAAGRPSAEAVGAARAAAGVAPSEAPDFRTEIERNYELARLIGATGTPTFVVGDKVL
                     QGAVGYDALKQAIADARGG"
     gene            complement(5128..5799)
                     /locus_tag="VF589_01585"
     CDS             complement(5128..5799)
                     /locus_tag="VF589_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX8256298.1"
                     /translation="MSSDKGLRTRVKTAKGRKVGSTRWLERQLNDPYVKRAKAEGYRS
                     RAAYKLLELDERFGFLRGVKRVVDLGIAPGGWSQVVRKKSPQAAVVGIDLLPADPIEG
                     VAILELDFMDESAPGRLREALGGPADLVLSDMAANTVGHPQTDHLRTMGLVEAALLFA
                     TEILRPGGAFVAKVLAGGADAQLVAELKRNFTAVKHAKPPASRKGSSEWYVVAQGFKG
                     APAAG"
     gene            complement(5796..6851)
                     /locus_tag="VF589_01590"
     CDS             complement(5796..6851)
                     /locus_tag="VF589_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX8256299.1"
                     /translation="MAQIAATAPQAPAPFPAHAAPADPALRRPPTRHTYGALDLGTNN
                     CRLLVARPSEDGFVVVDAFSRVVRLGEGLAASGRISDAAIDRALSALSVCADKLRRRR
                     VSLARSVATEACRRAVNGRHFVERVHRETGIALDIIAPEEEARLAMLGCHRLLEPGEG
                     PALIFDIGGGSTELVLVDTDGQETPRIKCWWSAPWGVVSLTEAEGRDFADADERLAAY
                     VRMRERVRHAFRRFAELLPKEKDDIRLLGTSGTVTTLASVHLALPSYDRRAVDGLLVP
                     ADSMREISRMMSRMSIEERAQLPCIGPERADLVVAGCAILEAILDIWPAATLGVADRG
                     IREGILRSLMARDGHPI"
     gene            7032..7568
                     /locus_tag="VF589_01595"
     CDS             7032..7568
                     /locus_tag="VF589_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8256300.1"
                     /translation="MPLAACLAACLAPCLAAAAPAPPAAPPGPAPAAGSFEARILAAH
                     NQYRAAVGVPPLRWEPRLAADAQRYGAQMAASGQLVHSPRASRPGQRENLWMGRAGSH
                     SAEAMIANWAQERSVFRPGIFPNISTTGDPGAVSHYSQVIWPATTHVGCAVNRARGRD
                     YLVCRYSPPGNIDGQRVG"
BASE COUNT         1245 a         2650 c         2681 g         1001 t
ORIGIN      
        1 cccggagaac attcaatgtc aatgcgcatg ctgatcgacg cgcgccatcc cgaggaaact
       61 cgggtcgccg tcgccaaggg aaaccggatc gaggaatttg atttcgaatc ggccgagcac
      121 aagcagctca agggtaatct ctatctagcc aaggtcacgc gggtcgagcc gtcgctgcag
      181 gcggcgttcg tcgattatgg cggcaaccgc cacggcttcc tcgctttctc cgaaatccac
      241 cccgattatt accaaatccc caaggaggat cgcgaggcgc tgctgcgcga ggaggccgag
      301 catgccgccg aggaagagcg gctgcgggcg gcggaggacg cgcgctacga cgacgacgac
      361 gattatgccg aaagctcggc cgagcgcgac tcggacgata tcgaggaagt cggcgacgat
      421 gccggcggcc ccagcgacga cgggcatgac gacgggcatg gcgacgacga gcatggcgac
      481 gaccatggcg atgacggcga aggccatgag gacgaggagg ggcgggcgcc gcccaagccg
      541 gaggccgcca gcggctccga ggagaatgcc gccgacgaat tgaggcgcaa gcgccagaat
      601 ctgcgccgcc gctacaagat ccaggacgtg atccgccgcc gccaggtgct gctggtccag
      661 gtcgtcaagg aagagcgcgg caacaagggc gcggcgctca ccacctatct gagcctcgcc
      721 ggccgctatt gcgtgctgat gcccaacacc agccatggcg gcgggatcag ccgcaagatt
      781 tcgaacggag ccgatcgcaa gcgcctgaag tcgatcatga cggaattgaa cctgccgcgg
      841 acgatgggct gcatcgtgcg caccgccggc ctctcccgca ccaagaccga gatcaagcgc
      901 gacttcgact atctcgcccg gctgtgggac gagatccgcg agcgcaccct cgggagctcc
      961 gcgccggctt tgatctacgg cgacagcgac ctcatcaaac gcgccatccg cgacatctac
     1021 aatcgcgata tcgacgaagt gctggtcgaa ggcgaagagg gctatcgtgc cgcccgctcg
     1081 ttcatgaaat tgctgatgcc gagccacgtc cggcgggtga agagctatgc cgacccggtg
     1141 ccgctgttcc agcgcttcgg cgtggaggac cagctcagcg gcatgtacca gccgctcgtc
     1201 cagctcaaat cgggcggcta tctggtgatc aacccgaccg aggctttggt gtcgatcgac
     1261 atcaactcgg gccgctcgac ccgcgagcat aatatcgagc agaccgctta cgcgaccaac
     1321 atcgaggcgg cgcacgagat tgcgcgccag ctccggctca gggacatggc cggcctcgtc
     1381 gtgatcgact tcatcgacat ggaatcgaac ggccacgtcc gcaaggtcga gaaagcgatg
     1441 aaggaggcgc tgaagaacga tcgcgcccgc atccaggtcg gccgcatctc ctccttcgga
     1501 ctgatggaga tgagccgcca gcggctgcgc accggcgtgc tcgaagcctc gaccaagccc
     1561 tgcccgcatt gcgagggctc cggcctgatg cgcacggcct cgtcggccgg gctgtcggcg
     1621 ctgcgcatgc tcgaggacga ggcggcgcgc gggcgcggca gccgcatcat cctgcgtgcc
     1681 gggcgcgagg ccgcggtcta tgtgctgaac cgcaagcgcg ccgagatcgg cgagatcgag
     1741 gcgcgctacg gcgtgatcgt cgaagtgctg atcgaggaag ccctcgaagg cgcgaagatg
     1801 agcgtcgaat cggtcggcct gccgccgctg cagcggccgc ggccggtgcc gatcgtcgag
     1861 cctttgatag atgaggagga ggatcttctc gaggaggaag aggaggaact cgacgaggag
     1921 gaagaggaag cggccgaaga gcccgaggcg ccgcgcgagg agcgccggcc gccccgtccc
     1981 gccgccgccg ccgaggactc ggagagcgag cgcggccgca agcgccgccg ccggcgcggc
     2041 cgccgcggcg gacgccgccg gcccggtgag gaagagggac gccgcgagga aggcggcgac
     2101 gagccgcagg tcgcgggcga ggacgagcgg atcgtagccg aggaagccgc ggccgaagcg
     2161 ccgatggcgg aggccgagcc tgagctcgaa gcccccaccg acgccggcat cgccgaggcc
     2221 cccgccgagg aggagaagcc gcgcaagggc cgccgccggc cgcgcgcccg ggaggccgag
     2281 gtatcggctc cggccgccgc accggccgaa gctccggctg ctgtcgaggc cgctccgatc
     2341 gccgaggctg ccccggccgc cgaggaggcg ccggccaagc cgcgccgcag ccggcgcaag
     2401 aaggccgatc ccgaagccga agctccggcg gaagcacccg cgcctgccgt cgaggaagct
     2461 gctgcgccgg ctcccgagga gacgggtgcg cccgcggccg ccaacgacac ggccgaggcc
     2521 gcgccggcgc ccaagcgccg cagccgcgcc aagaaggtcg ccgaggcgcc cgaggccccg
     2581 gaggctccgg tcctggagcg ggtggccgcc gacgggccgg acgccgacgg cggcgaggcg
     2641 gccgagagcg gcccgccgcg ccgcggctgg tggcagcgga cgttcggcgc ctgagccggc
     2701 gcgcggccgg gaaggcgtgc ggccgcggcg gctttggccg ccgccgccgc agccggcccg
     2761 ccgccgcgat ttcatcttcg gttcatgggc cggacaggat atcttgcgct gatggctgac
     2821 gcttccctat cgcggctgcg cgaaacccgg tccggctcgg ccggctggct cgcccggctg
     2881 atcctgtgcc tgacgctggc cttcgcgctc accgcccagc cggcgatggc gcagtcgctg
     2941 ctgcgcgatg ccgagaccga ggccttgttc gacgaaatgg cgcggccgct gatcgaagca
     3001 gcggggatgc ggcccgaaaa cgtcgaggtc gtgctgatcg gcgacaaatc gatcaacgcc
     3061 ttcgtcgccg gcggccagat cgtctacatc cactcgggcc tgatcaccgc ggcggaaaac
     3121 gccaacgagg tgcagggcgt gatcgcgcac gagctcggcc acatcaccgg cggccatatc
     3181 atccgcctcg gcgagggcgc gcgggtcgcc accgggatca gcatcctgtc gctgctgctc
     3241 ggcgcggcgg cgatggcggc gggggcgggc aatgccggca ccgcgatcat ggcggccggc
     3301 cagcaggcgg cgatgggcaa gttcctcgcc ttcacccgca cccaggaatc gtccgccgac
     3361 caagccgggg cgagctacct ccggaaggcc ggcatcaccg gcaagggctc gctctccttc
     3421 ttcaagaagc tccagaacca ggaattccgg ctcgccatcc cccagcagga cagctacgcg
     3481 cgcacccatc cgctgaccgg cgagcggctc gccgcgctcg agaatgtcta cacgaccgac
     3541 gccgcctggc agaagccggc cgataccgcg cttgaagcac gcttccagcg ggtcaaggcc
     3601 aagcttgccg gctatgtcga cgaaccgcgc cagaccctga tcaaatatcc cgagaccgac
     3661 cgcaccgtcc ccggccgcta cgcgcgcgct tatgcgtacc acaagagcgc ctatccggac
     3721 cgcgccgccg ccgaggtcga cagcctgctc cgcgactatc cggacgatgc ctatttcctc
     3781 gaactgaagg gccagatcct gctcgaatcg ggcaagccga aggaagcgct cgaatcgctg
     3841 cgcaagtcgg tgcggctcgc gcccaaccag ccgctcatcg cctcgacgct cggccatgcc
     3901 ttgatctcga ccgaggacgc caagaatttc gccgaggcca agcaggtgct gcgcgccgcg
     3961 gtcggccgcg acaattcgaa cccgttcgcc tggtaccagc tcggcatcgt ctacgaccgc
     4021 gagggcgatc cggcgcgggc ggcgctcgcc accgccgagc gtcacagtct cgaaggcaat
     4081 ccgctgcagg cgatggccag cgcgcaactc gccatggcgg gggtgaagcc gggcacggcc
     4141 gactgcctgc gcgcgcagga tatcgccatg gcctcgcgcg cctcgatcct ccagaaccgc
     4201 agggaatcga cggaggaatc gcgcgtcgcc aagatgatcg agcgcaacga gcggcggagc
     4261 ggcgcgccct cgatggcgtg cggcggcgct tgaccggcag gaacctggcg atcgcggcgg
     4321 gcgcggccgg gctgctggcg ggagccggcg cggtcctcct cgcgcgcggc ggcggcgacg
     4381 ggctggtcgg cggcgagcgc gcacaggtcg aggcgacggt gcgcgattat atcctcgcca
     4441 atcccgagat catcccggaa gcgatgaagc ggctccagga ccgtcagctc ggcaaggcgg
     4501 tcgccgacaa tcgccaggcg ttcgagacgc cgttcggcag cgcctgggcg ggggccgaga
     4561 acgccgacgt cgtgctggtc gaattttacg attatgcctg cggctattgc cgcaagaccg
     4621 tgtcggacct cgagcgtctg ctcgccgagg atgacggcct caaggtggtg tggcgcgagc
     4681 tgccggtgct gggcccggac agcgaggcgg ccgccatggt cagcctcgcc gccgcgcgcc
     4741 agggcaagtt caaggcgttc cacaaccggc tgttcgcggc cggccggccg agcgccgaag
     4801 cggtcggcgc ggcccgggcg gcggccggcg tggcaccgtc cgaggcaccc gatttccgca
     4861 ccgagatcga gcgcaattac gagctcgccc gcctgatcgg cgcgaccggg acgcccacct
     4921 tcgtcgtcgg cgacaaggtg ctgcagggcg ccgtcggcta cgacgcgttg aagcaagcca
     4981 tagccgacgc gcggggcggt tagtccgaga cgcccaccgt catcccagcg aaagctggga
     5041 tctcacttct tcttcccaca cccgatcaag gaaagagagt ttccagcttt cgctggaatg
     5101 acgaggccct ggctccgcgc aagccgctca gcccgccgcc ggcgcgccct tgaacccctg
     5161 cgccaccaca taccattccg acgagccctt gcggctggcg ggcggcttgg cgtgcttgac
     5221 ggcggtgaag ttgcgcttga gctcggcgac caattgcgcg tcggcgccgc cggccagcac
     5281 cttggcgacg aaggcgccgc cggggcggag gatctcggtc gcgaacagca gcgccgcctc
     5341 aacgaggccc atggtgcgca gatggtcggt ctgcggatgg ccgaccgtgt tggcggccat
     5401 gtcggagagg acgaggtcgg ccggcccgcc gagcgcctcg cgcaggcggc cgggcgcgct
     5461 ttcgtccatg aagtccagtt cgaggatggc gacgccctcg atcggatcgg cgggcagcag
     5521 gtcgatgccg accaccgccg cctgcggcga ctttttccgc acgacctggc tccagccgcc
     5581 cggcgcgatg ccgaggtcga ccacccgctt cacgcccctc aaaaaaccga accgctcgtc
     5641 gagctcgagc agcttgtagg cggcgcggga gcggtagccc tccgccttgg cgcgcttgac
     5701 ataggggtcg ttcaactgcc gctcgagcca ccgcgtcgac ccgaccttgc gccccttggc
     5761 cgtcttgacg cgggtccgaa ggcctttgtc cgaactcata tggggtggcc gtcgcgggcc
     5821 atcagcgagc gcaatatgcc ctcgcgaatg ccgcgatcgg cgacgcccag ggtcgcggcc
     5881 ggccagatgt caaggatcgc ttcgaggatg gcgcagccgg cgaccaccag gtcggcgcgc
     5941 tccggcccga tgcagggaag ttgcgcgcgc tcctcgatcg acatgcgcga catcatccgg
     6001 ctgatctcgc gcatcgaatc ggccgggacg agcaggccgt cgaccgcgcg ccggtcgtag
     6061 ctcggcagcg cgagatggac gctggccaaa gtggtgacag tgccgctggt gccgagcagt
     6121 cggatgtcgt ccttctcctt gggcagcagc tcggcgaagc ggcggaaggc gtggcggacg
     6181 cgctcgcgca tccggacgta ggcggcgagc cgctcgtcgg cgtcggcgaa gtcgcgcccc
     6241 tcggcctcgg tcagcgagac cacgccccag ggcgcgctcc accagcattt gatgcgcggc
     6301 gtctcctggc cgtccgtgtc gaccagcacc agctcggtcg aaccgccgcc aatgtcgaag
     6361 atgagcgccg gcccctcgcc gggctcgagc agccggtggc agccgagcat ggccagccgc
     6421 gcttcctcct cgggcgcgat gatgtcgagc gcgatgccgg tctcgcggtg gacgcgctcg
     6481 acgaaatggc ggccgttgac ggcgcggcgg caggcctcgg tcgcgaccga gcgggccagc
     6541 gagacgcggc ggcggcgcaa tttgtcggcg cagaccgaca gagccgacaa ggcgcggtcg
     6601 atcgcggcgt cgctgatccg gccgctcgcg gcgagccctt cgccgagccg gacgacgcgc
     6661 gaaaaggcgt cgacgacgac gaagccgtcc tccgacgggc gcgcgaccag cagccggcaa
     6721 ttgttggtgc cgagatcgag cgcgccatag gtgtgacggg tgggaggccg ccgcaaagcg
     6781 gggtccgcgg gggccgcatg ggccgggaaa ggggccggcg cctgcggagc cgtggcggca
     6841 atctgcgcca tgctcttaca ctcttatgtt ctcgttcagg cggcgacacc gccgctttgg
     6901 cttggcgcct gcttagcgcg aaaccgcttt ttcggcaagc ggccgggccg atggcgcgcc
     6961 ggcggcggat agggtaaggc cgagtccgtc catcgaaagg agcggcgcgc atgatccgaa
     7021 ggcgtctcgg aatgcctctg gcggcctgcc tcgccgcctg cctcgccccc tgccttgccg
     7081 ccgccgcgcc ggcaccgccg gccgcgccgc ccggcccggc gccggcggcg ggcagcttcg
     7141 aagcgcggat cctcgccgcc cacaaccaat atcgcgccgc cgtcggcgtc ccgccgctgc
     7201 gctgggagcc gcggctcgcc gccgatgccc agcgttatgg cgcgcagatg gccgcctccg
     7261 ggcagctcgt ccattcgccg cgcgcctcgc ggccgggcca gcgcgagaat ctctggatgg
     7321 gacgggcggg ctcccacagc gccgaggcga tgatcgcgaa ttgggcgcag gagcgcagcg
     7381 tcttccgccc aggcatcttc cccaacatca gcacgaccgg cgatccgggc gcggtcagcc
     7441 attattccca ggtcatctgg ccggcgacca cccatgtcgg ctgcgccgtc aaccgcgcgc
     7501 gcgggcggga ctatctcgtg tgccgctatt ccccgcccgg caatatcgac ggccagcggg
     7561 tcggctgacg cctattg
//
LOCUS       DASVRK010000042         5984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_504574, whole genome shotgun sequence.
ACCESSION   DASVRK010000042 DASVRK010000000
VERSION     DASVRK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504574"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1191)
                     /locus_tag="VF589_01600"
     CDS             complement(118..1191)
                     /locus_tag="VF589_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEX8256301.1"
                     /translation="MAAAVRHWTWAIPVAAWLLLSVGLGASIPTLILAMVLIATVLAA
                     VHHAEVVAHKVGEPFGTFLLAIAVTIIELGLILTLMSAAGEGAAGLARDTVFAAVMII
                     LNGLVGVCIFVGAIRHKEQIFQQTGVSASLATICALTVLTLVLPNFTVSEAGPVYSPS
                     QLGFVAVVSFLLYATFVAVQTVRHRDYFLPDRALAADEQIHAAPPSSRQTLLSGVLLL
                     ACLGAVVLLAKSLAPPIEAAVATAGAPRAIVGVIIAALVLLPESVAALRAALANRLQT
                     SLNLGLGSALATIGLTIPAVAALSLMAGLPIALGLDAKSTVLLFLTLIVSTLTLGTGR
                     TTVLQGGVHLVIFAVYLFTTIVP"
     gene            complement(1256..1978)
                     /locus_tag="VF589_01605"
     CDS             complement(1256..1978)
                     /locus_tag="VF589_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX8256302.1"
                     /translation="MIETSVKSYCRAVALFLILSMVGGWFGEMHVPSLMMTGDAASTA
                     DQLRLNEGLFRLGFAAYLLEAFSDVVLAWLFYVLLKPVHRDLALLSAFFGLVSMSMFA
                     VPKLLYFAAPMFLKGSGYLAAFPQGQLEALASIFLSLYGVLGGISMLFYGTGWILRGY
                     LTFGSGYLPRFLGAIMVVAGLGFVAKTITQVLAPAYSWDLLLAPMFLNLIALVVWMLA
                     KGVDRDKWIGATECHEATGPAR"
     gene            complement(2077..2814)
                     /locus_tag="VF589_01610"
     CDS             complement(2077..2814)
                     /locus_tag="VF589_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256303.1"
                     /translation="MPVSIRKKIALALGCVVALVAAAILYPQPLFAYSKDYGAFTVYS
                     DRPIDPAMARVVEDAERRLRSSELYRPGEPFRVFVCNSPWRLLLLARSMSVGGSADPL
                     FTRNIYLREADAAANRVLIGDAVLADAEARPLSYFIAHEAAHVLQSRRFGRFMALRYP
                     RWLIEGHADLVAKAGDFDGTSNRALLNEGDGLLAEEYARTGLYRRYHLMVLSLMEQSG
                     SNETELFEAPPSQQDALKAAAGQHRVR"
     gene            2975..4972
                     /locus_tag="VF589_01615"
     CDS             2975..4972
                     /locus_tag="VF589_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8256304.1"
                     /translation="MADEDTDAGPEEESGSWRRGWATGGAIVATILLILLIVVVTLSN
                     RNRDDALRWERHTYDVMLLTRTVDATLARSEAALGRFVLDERRESGGLYYMEWRRAGR
                     QIGQLYRLARDPEQKARVEELRQLYQKRGQELAPAATRAAAQRGNDALAFFYQAGISA
                     TGPALRDKLDTIAAGERDNLRQRMADTRLFAEQADKFTEWLAWLGILIGVGAIGLGLY
                     AYRALADRVLARQQADSEAHRAMALERAVQDRTRELRDANERLRAEAAERAAAEAQLR
                     QVQKMEAVGQLTGGIAHDFNNMLAVIVGGLDLAKRKLRGPKRDVEFHLDNAMEGATRA
                     AALTRRLLAFARSEALLPKGVEPAALVEGMLDLIDRSIGERIAVQTRFPDDQWNVFAD
                     ANLLENAILNLCVNARDAMSGGGQLDIAVENVVLRPGEIGNLAAGDYVRISVADNGVG
                     IPPEHLDRVFEPFFTTKPVGKGTGLGLSQIFGFARQSGGDVAIDSTPGAGTIVSIYLP
                     RSTRSADAADVEGAGGQEPARAPAGTSILVVEDDPRVSRSTVGALEELGYAPVACASG
                     AEALAILEREDGIELVITDVMMPEMTGPELIRIVGERWPHIAILFVTGFVGEAGEAEG
                     LAGYDILRKPFTVAALSDAVARALAHRLSVSHPASASAAAE"
     gene            complement(4929..5858)
                     /locus_tag="VF589_01620"
     CDS             complement(4929..5858)
                     /locus_tag="VF589_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256305.1"
                     /translation="MSDVRPLSELVAAELAEPVDPRVAAMAAALAGRYPGAAWAVLFY
                     GSCLRTAALEGQMLDFYLIVSDYRAAYGRSWLARANRLVPPNVFPFEHAGLAAKYAVL
                     SEADFERECGPRARSVSVWARFAQPSRLAWAADEDARRRVTAAVAEAAPTLFQLTLPM
                     MDLRAPVAPLDIWKKGFSLTYGAELRAERTDRSAAIVDADPERYERFGLAALGAIAAS
                     RAVGPGGEVPLIASPDRMRRRWRGMQRKGKALSVVRLAKASATFAGGVDYIAWKINRH
                     AGTQIVLKPWQRRWPLLAAVTLLPRLLKRGAIR"
BASE COUNT         1058 a         2153 c         1958 g          815 t
ORIGIN      
        1 ctgcgctgct cgctctgcgc cgaccacacg gccgagcagg tgacccatat cctcgaccgc
       61 ttcgagaccg ccggccgcgc caccggctgc atcgtctgat cccgtcgcac cgcggcgcta
      121 cggaactatc gtcgtgaaca aatagaccgc gaagatcacc aggtgcacgc cgccttggag
      181 gacggtggtg cgtcccgtcc ccaaggtgag cgtcgacacg atcagcgtga ggaagagcag
      241 cacggtgctt ttcgcatcga gcccaagcgc gatcggcagg ccggccatca gcgacagcgc
      301 ggccaccgcc gggatggtga gcccgatcgt cgcgagcgcg gagccgaggc cgagattgag
      361 gctggtctgg agccggttgg cgagcgccgc ccgcaacgcc gcgacgcttt cgggaagcag
      421 gaccagcgcg gcgatgatca cgccgacgat cgcccgcggc gcaccggcgg tcgcgacggc
      481 ggcttcgatc ggcggcgcga gcgacttggc gagcagcacc acggcgccga ggcaggccag
      541 cagcaggacg ccggacagca aggtctgccg gctcgagggc ggcgcggcat ggatctgctc
      601 gtccgcggcc agcgccctgt ccggcaggaa atagtcgcgg tggcggacgg tctggaccgc
      661 cacgaaggtc gcatagagca ggaacgagac gacggcgacg aaacccagct gcgacggcga
      721 atagaccggt ccggcctcgc tcacggtgaa gttgggaagg accagggtca ggaccgtcag
      781 agcgcagatc gtcgccagcg aggcgctgac cccggtctgc tggaagatct gttccttgtg
      841 ccggatggcg ccgacgaaga tgcacacccc caccagcccg ttgaggatga tcatcaccgc
      901 ggcgaacacg gtgtcgcggg ccagcccggc cgcgccctcg cccgccgccg acatcaaggt
      961 gaggatgagc cccagctcga tgatggtgac ggcgatcgcc agcaggaacg tcccgaacgg
     1021 ctcgcccacc ttgtgcgcca ccacttcggc gtggtggacg gcggccagca cggtcgcgat
     1081 cagcaccatg gccaggatca gcgtggggat cgacgcgccc aggccgacgc tcagcagcag
     1141 ccacgccgcg acgggtatcg cccaggtcca gtgtctgaca gccgcagcca tgccgcgtct
     1201 aatagtgctt gggcggcggt cggcaaacgg cactttgccg ctcgtcatcc cggcctcagc
     1261 gggcgggtcc ggtcgcttca tggcattcgg tcgccccgat ccatttgtcc cgatccacac
     1321 ctttcgccag catccagacg accagggcga tcaggttgag gaacatcggc gccagcagga
     1381 ggtcccacga ataagccggg gcgagtacct gcgtgatggt cttggccacg aagccgaggc
     1441 cggcgactac catgatcgcg ccgaggaagc gcggcagata tcccgatccg aaggtcagat
     1501 agccgcgaag gatccacccg gtgccgtaga acagcatcga aatcccgccg agcacgccgt
     1561 agagcgacag gaagatgctc gccagcgcct cgagctgacc ctgcgggaag gccgccagat
     1621 agccgcttcc cttgaggaac atcggcgccg cgaaatataa cagcttcggc accgcgaaca
     1681 tcgacatcga gaccagcccg aagaaggccg acagcagcgc caggtcgcga tgcaccggct
     1741 tcaggagcac atagaatagc caggccagca ctacgtcgct gaaggcttcg aggaggtagg
     1801 ccgcgaagcc cagcctgaac aaaccctcgt tgagcctgag ctggtcggcg gtgctcgcgg
     1861 cgtcgccggt catcatcagc gacgggacgt gcatctcccc gaaccagccg ccgaccatcg
     1921 acaggatcag gaacaaggcg accgcgcggc agtagctctt gacggatgtc tcgatcatct
     1981 ccggcggccc ctttggcagc gtctcggggc cagtcgaacg tcacacggcc attgtattac
     2041 taacataaca cacgtcaggc gaaacgccaa accgggtcag cggacccgat gctgccctgc
     2101 agccgctttt aaagcgtcct gctgggacgg cggcgcctcg aatagctcgg tctcattgct
     2161 cccgctctgc tccatcagcg acagcaccat cagatggtag cgccgataca gacccgtgcg
     2221 ggcatattcc tcggcgagca gaccgtcgcc ctcgttcagc agggcccggt tgctcgtccc
     2281 gtcgaaatcg cccgccttcg caaccaggtc ggcatggcct tcgatcagcc atcgcggata
     2341 gcgcaaggcc atgaagcgcc cgaagcggcg cgactgcagc acatgcgccg cctcgtgcgc
     2401 gatgaaatag gacaagggac gggcctcggc atcggcgagc accgcatcgc cgatcagcac
     2461 ccggttcgcc gcagcgtccg cctcgcgcag gtagatgttg cgcgtgaaca gcgggtccgc
     2521 cgacccgccc accgacatgc tcctggcgag gaggagcagc cgccacggcg aattgcagac
     2581 gaagacgcgg aacggctcgc ccggacgata aagctccgac gaccgcagcc gccgctcggc
     2641 gtcctccacg acccgcgcca tcgccggatc gatcgggcgg tccgagtaga cggtgaaggc
     2701 gccatagtcc ttcgaatagg cgaacaaagg ctgcggatac agaatggcgg ccgccacaag
     2761 cgcgacgacg cagccgaggg ccagggcgat ctttttgcgg atagagaccg gcacagcacc
     2821 tcgaacggaa tgagcctcgc ttgcatccca tggccgcgcg gcgtccgcaa tggctcggtc
     2881 gcggacattt ggcgccagcg atcttcggac tgacccgctc cctaagaacc aaaagtcttt
     2941 caacgggctg cggcattcac tagggtggcc ggctatggcg gatgaagaca ctgacgccgg
     3001 tccggaagag gaatccggtt cgtggcgccg gggatgggcg accggcggcg cgatcgtcgc
     3061 gaccatcctg ctcatcctgc tcatcgtggt cgtcaccctc tccaaccgaa atcgcgacga
     3121 tgccttgcgg tgggagcggc acacttatga cgtaatgctg ctgacccgca ccgtcgacgc
     3181 caccctcgcc cgctccgagg ccgcgctcgg ccggttcgtg ctcgacgagc ggcgcgaaag
     3241 cggcggcctc tattacatgg agtggcgccg ggccggccgg cagatcggcc agctctaccg
     3301 cctcgcccgc gatcccgagc agaaggcgcg ggtcgaggag ctccgccaac tctaccagaa
     3361 gcgtggccag gagctggcgc cggccgccac ccgcgccgcc gcccagcgcg gcaacgacgc
     3421 gctcgccttt ttctaccagg ccggcatctc cgcgaccggc ccggcgctgc gcgacaagct
     3481 cgacaccatc gccgccggcg agcgcgacaa tctgcgccag cgcatggccg atacccgcct
     3541 gttcgcagag caggccgaca agttcaccga atggctggcc tggctcggca tcctgatcgg
     3601 cgtcggcgcg atcggcctcg gcctctacgc ctatcgggcg ctggccgacc gcgtgctcgc
     3661 ccgccaacaa gccgacagcg aggcgcaccg cgcgatggcg ctcgagcggg cggtgcagga
     3721 ccgcacgcgc gagcttcgcg acgccaacga gcgcctgcgc gccgaagcgg ccgaacgcgc
     3781 cgccgccgag gcccagctcc gccaggtcca gaaaatggag gcggtcggcc agctcaccgg
     3841 cggcatcgcc cacgacttca acaacatgct cgcggtgatc gtcggtggcc tcgatctagc
     3901 caagcgcaag cttcgcggcc ccaagcgcga cgtcgaattc catctcgaca acgccatgga
     3961 aggcgccacc cgcgccgccg cccttacccg ccgcctgctc gccttcgccc ggtcggaggc
     4021 gctgctgccc aaaggggtgg agccggccgc gctggtcgaa ggcatgctcg acctcatcga
     4081 ccgttcaatc ggcgagcgca tcgccgtcca gacccgcttc ccggacgatc agtggaacgt
     4141 cttcgccgat gccaacctgc tcgagaatgc gatcctcaat ctctgcgtca acgcccgcga
     4201 cgcgatgagc ggcggcggcc agctcgacat cgcggtcgag aatgtcgtgc tgcgcccggg
     4261 cgaaatcggc aacctcgccg ccggcgacta tgtccggatc agcgtcgcgg acaatggcgt
     4321 cggcatcccg cccgagcatc tcgaccgcgt gttcgagccc ttcttcacga ccaagccggt
     4381 cggcaagggc accggtctcg gcctcagcca gattttcggg ttcgcgcgcc agtcgggcgg
     4441 cgacgtggcg atcgattcca cccccggcgc cggcaccatc gtatcgatct acctgccgcg
     4501 ctccacccgg agcgccgacg ccgccgacgt ggagggcgcc ggcggccagg agccggcgcg
     4561 cgcgcccgcc ggcaccagca tcctggtggt cgaggacgac ccgcgcgtca gccgctccac
     4621 cgtcggcgcg ctggaggagc tcggttatgc ccccgtcgcc tgcgccagcg gcgccgaggc
     4681 gctcgccatt ctcgagcggg aggacgggat cgagctcgtc atcaccgatg tgatgatgcc
     4741 cgaaatgacc ggacccgagc tgatccggat tgtcggcgaa cgctggccgc acatcgccat
     4801 cctgttcgtc accggcttcg tcggcgaggc cggcgaggcc gaggggctgg ccggctacga
     4861 catcttgcgg aaaccgttca ccgtcgccgc tttgtccgac gccgtcgccc gcgcgctcgc
     4921 ccaccggctc agcgtatcgc accccgcttc agcaagcgcg gcagcagagt aacggcggcc
     4981 agcagcggcc agcgccgctg ccacggcttc agcacgatct gcgtgcccgc atgccggttg
     5041 atcttccagg cgatgtaatc gaccccgccg gcaaaggtgg cgctcgcctt ggcgaggcgc
     5101 acgaccgaca gcgccttgcc cttgcgctgc attccccgcc agcgccgccg catccggtcg
     5161 ggcgacgcga tcaacggcac ctcgccgccc ggcccgacag ccctggaagc cgcgatcgcg
     5221 ccgagcgccg ccagcccgaa gcgctcgtaa cgctccggat cggcgtccac gatcgccgcc
     5281 gagcggtcgg tccgctcggc cctcaattcg gcgccgtagg tgagcgagaa acccttcttc
     5341 cagatgtcga gcggcgccac cggcgcgcgc aggtccatca tcggcaaggt gagctggaac
     5401 agggtcggcg ccgcctccgc caccgccgcc gtcaccctgc ggcgcgcatc ctcgtccgcc
     5461 gcccaggcca gccgcgacgg ctgcgcgaaa cgcgcccaga cagagacgct gcgcgcgcgc
     5521 ggcccgcact cgcgctcgaa atcggcctcg ctcagcaccg catatttggc ggcgaggccg
     5581 gcatgttcga acggaaagac gttgggcggg accagccggt tggcccgcgc cagccaggag
     5641 cgcccgtaag ccgcgcgata atccgacacg atcaggtaga aatcgagcat ctgcccttcg
     5701 agcgcggccg tccgcaggca cgacccgtag aataataccg cccacgccgc gcccggatag
     5761 cgcccggcca gcgccgccgc catcgcggcg acgcgggggt cgaccggctc ggcaagctcg
     5821 gcggcgacca gctcggacag gggacggaca tcgctcacgc ccccactctt agcgaggcgg
     5881 cggcggttgc gggagccccc cgccgtcgtc ggcaggctcg ctgattcgga ctaacctgta
     5941 tcagtctccg ctcgtcccgc ccggccgcgg cggcgggaaa atac
//
LOCUS       DASVRK010000043         5211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_504592, whole genome shotgun sequence.
ACCESSION   DASVRK010000043 DASVRK010000000
VERSION     DASVRK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504592"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VF589_01625"
     CDS             <1..441
                     /locus_tag="VF589_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEX8256306.1"
                     /translation="TIDSVDGYRRSAQEATSSRFSSDFLERANEREQVVSKLRDEVRR
                     LGGNPEDDGSVLAAAHRAFLGLRDKLTGSDDAAVIAEVDHGESYLNGKWETALRDDQL
                     APETRALIQQCYDSVREGRDTFRRLHEDMSASTTSTTTGGGSGF"
     gene            complement(497..1030)
                     /locus_tag="VF589_01630"
     CDS             complement(497..1030)
                     /locus_tag="VF589_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256307.1"
                     /translation="MRAPILALLALAACDGGVPAAPAAPEPASIPQPSGPPAPQNLPD
                     PVPPAAPVQPGGWESAASGEGIALRLTAPGGKLVMSIACLGDPVRLSVAVPGFTPIGS
                     EDRFALALGEEPATLVAGPTRQSEGVTAEGPVPETFADAFGEAAQIGALYGTQRVGPA
                     PPPPDKLARLLIEACSA"
     gene            complement(1069..2151)
                     /locus_tag="VF589_01635"
     CDS             complement(1069..2151)
                     /locus_tag="VF589_01635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8256308.1"
                     /translation="MILRELAMDKKRAEPAVEVTLAPGLPDEIDAVAGRADPRHAFLR
                     RAWFAAARPEAPATLLAGRADGRLIAALPLGRTGPRLLGWKAVPGCYWPYRSFPVAAD
                     AGDAELGALLAHPVARQALGPVWRLGPVYADDPTAGRLARLATSSGWTPLPRRIATAF
                     HFDMAAQRAAGAWPRTSTLKKNRFFEKHLGQSGELGFRFVSGAEWTPEIFDSLASVEA
                     RSWIPKETGGGDAKFLAPHHRRFWEEAARDPAIAAMLHAAILTIGGEPVAFAFDLDAG
                     TLKYAIANSYDEAFAKRSPGRVLAYRSLPAAAERGIEQVDWGAGDSGYKRTLGAEAGP
                     EILDYLFVRSRALSALLRPWWTRSGR"
     gene            2252..2722
                     /locus_tag="VF589_01640"
     CDS             2252..2722
                     /locus_tag="VF589_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX8256309.1"
                     /translation="MTGASLSNVAEIIQLAVAPVFLLAGIGGILNVIAQRLARVVNRV
                     RDLEKDVPTADPELQAAELGELAILDRRMTLCHWSIGLSTAAALLICLVVMVLFIADL
                     AALEFAVPVSLLFIGAMASLTLGLLLFLYEVTVATRSVRVSDKFVTRRVRRRAR"
     gene            2712..3125
                     /locus_tag="VF589_01645"
     CDS             2712..3125
                     /locus_tag="VF589_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HEX8256310.1"
                     /translation="MRADAERRLLQGAVVLASLVPLSMGLMSVIEGASVIRGVGAPTT
                     DLDSHFRYLSGLLLGIGLAYLAALPRIERSTTLFRLLGGIILVGGLARLLSLAEDGAP
                     SEGHVFGLAMELGVVPAITLWQSRIARRFSGPGKK"
     gene            complement(3404..3931)
                     /locus_tag="VF589_01650"
     CDS             complement(3404..3931)
                     /locus_tag="VF589_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256311.1"
                     /translation="MRRVRDWVVLGLLAGAAACAPQRRPPPPAPPQQQPRPPQPQPAR
                     PLPPPPPIADWRDVPLTPGGWVYRAEGAGPSAAFGQAAGGPAFTVRCDRAGRQVLLAR
                     GGGGPGNAITIRTSTLSRSLALSGGSAALPARDPLLDAIAFSRGRFTVEQAGLPMLVV
                     PSWPEPARVIEDCRS"
     gene            complement(4058..4543)
                     /locus_tag="VF589_01655"
     CDS             complement(4058..4543)
                     /locus_tag="VF589_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256312.1"
                     /translation="MRSFALFPALLLPLALALAGCGESVKDDHFAKDLRDDREGEATA
                     RTPAAADAAPVRIGELGPSFAACNGAGTPRNLAAGETLPVRSAPFDHAAQTGSIAAAS
                     RFFVCTRSHDQRWLGVVYDEAGALDPRCGVAAPVASRRAYEGPCRSGWAPAASVKLVA
                     G"
     gene            4661..5056
                     /locus_tag="VF589_01660"
     CDS             4661..5056
                     /locus_tag="VF589_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX8256313.1"
                     /translation="MADEAEPGSYGWADLVRDGGAEWDGVRNPAARLHLKAMAAGDEA
                     FFYHSGADKAVVGVMRIARAAAPDPKDPAWVSVRVEPLRPLPAPVTLKAIKAEPALAA
                     MELIRQSRLSVSPVRGEEWEAILGLSARA"
BASE COUNT          754 a         1840 c         1915 g          702 t
ORIGIN      
        1 accatcgaca gcgtcgacgg ctatcgccgc tcggcccagg aagcgaccag cagccggttc
       61 agctccgact tcctcgagcg tgccaacgag cgcgagcagg tcgtcagcaa gctgcgcgac
      121 gaggtccgtc ggctcggcgg caatcccgag gatgacggca gcgtgctcgc cgccgcccac
      181 cgcgcctttc tcggcctgcg cgacaagctc accggaagcg acgacgccgc ggtgatcgcc
      241 gaagtcgacc atggcgagag ctatctcaac ggcaaatggg aaaccgcgct tcgcgacgac
      301 cagctggcgc ccgagacgcg cgcgctgatc cagcaatgct acgattccgt ccgcgaaggc
      361 cgcgacacct tccgccgcct ccatgaggac atgtcggcat cgacgacgtc cacgacgacc
      421 ggcggcggca gcggcttcta agcctcgcac ccgacgcaag acaggggcgc gccttcgggc
      481 gcgccctttt ttgctgtcag gccgagcagg cctcgatgag caggcgcgcc agcttgtccg
      541 gcggcggcgg cgccggcccg acgcgctgcg tgccgtaaag ggcgccgatc tgggccgcct
      601 cgccgaacgc gtccgcgaac gtctccggca ccggcccttc ggcggtgacg ccctcgctct
      661 gccgggtggg gccggcgacc aaagtggccg gttcctcgcc gagcgccagc gcaaagcggt
      721 cctcgcttcc aatcggcgtg aagcctggca cggcgacgct cagccgcacc ggatcgccga
      781 ggcaggcgat gctcatcacc agcttgcccc ccggcgccgt cagccggagc gcaatcccct
      841 ccccgctcgc cgcgctctcc cagccgcccg gctgcacagg cgcggccggc gggaccggat
      901 cgggaaggtt ctgcggcgcc ggcggcccgg aaggctgcgg aatgctcgcc ggctcgggcg
      961 cggccggcgc ggcgggcacg ccgccatcgc aggcggcgag cgccaacagg gccaggatcg
     1021 gtgcgcgcat catggccgca caacggcgcg gcatgagccg cgttccggtc agcgccccga
     1081 gcgcgtccac caggggcgga gcagggcgga gagcgcgcgg ctgcgaacga acagatagtc
     1141 gaggatctcc gggccggcct cggcgccgag ggtgcgcttg tagccgctgt cgccggcgcc
     1201 ccaatcgacc tgctcgatgc cgcgttccgc cgccgcgggc aggctgcgat aggcgagcac
     1261 ccggcccggg ctgcgcttgg cgaacgcctc gtcatagctg ttggcgatgg catatttgag
     1321 cgtgccggcg tcgagatcga acgcgaacgc gacaggctcg ccgccgatgg tgaggatcgc
     1381 ggcatggagc atcgcggcga tcgccgggtc cctggcggcc tcctcccaga agcggcggtg
     1441 gtgcggggcg aggaacttgg cgtcgccgcc cccggtctct ttcgggatcc agctccgcgc
     1501 ctcgaccgag gcgaggctgt cgaagatttc gggcgtccat tccgccccgg agacgaaccg
     1561 aaagccgagc tcgcccgact ggccgagatg cttttcgaag aagcggttct tcttgagcgt
     1621 cgacgtgcgc ggccaggcgc cggcggcgcg ctgcgcggcc atgtcgaaat gaaaggcggt
     1681 ggcgatgcgc cggggaagcg gtgtccagcc gctcgaggtg gcgagccggg ccaggcggcc
     1741 ggcggtggga tcgtcggcat agacggggcc gagccgccac accggcccga gcgcctgccg
     1801 cgccaccggg tgcgcgagca aggcgccgag ctcggcatcg ccggcatcgg ccgcgaccgg
     1861 gaagctgcga tagggccaat agcagcccgg aaccgctttc cagccgagca gccgcggccc
     1921 ggtccggccg agcggcagag cggcgatcag gcggccgtcg gcgcgcccgg cgagcagggt
     1981 ggcgggagcc tcggggcgcg cggccgcgaa ccaggcccgg cgcaggaacg catggcgcgg
     2041 gtcggcgcgc ccggcgacgg cgtcgatctc gtccggaagc ccgggggcga gcgtcacctc
     2101 gaccgcgggt tcggcccgct tcttgtccat cgccagttcg cgcaaaatca caatttcccg
     2161 gcagccattc gatggcagcg ttggtagcag ccaaaagcta ccatcgcgta agccgcttga
     2221 cgcgccgcgc ccgccgccgc ttaaccgccg catgaccggc gccagcctct ccaacgttgc
     2281 cgagatcatc cagctcgcgg tcgcgccggt gttcctgctc gccggcatcg gcggcatcct
     2341 caacgtcatc gcccagcggc tggcgcgggt ggtcaaccgc gtccgcgacc tcgagaagga
     2401 cgtgccgacc gccgatcccg agctgcaggc ggccgagctc ggcgagcttg ccatcctcga
     2461 ccggcggatg acgctctgcc actggtcgat cggcctttcg accgcggccg ccttgctgat
     2521 ctgcctcgtc gtgatggtgc tgttcatcgc cgacctcgcg gcgctcgaat tcgcggtgcc
     2581 ggtgtcgctg ctgttcatcg gcgcgatggc gagcctgacg cttgggctgc tgctgttcct
     2641 ctacgaggtc accgtggcga cgcggtcggt gcgggtcagc gacaagttcg tgactcggcg
     2701 cgtgcggcgc cgtgcgcgct gacgccgagc gccggctgct gcaaggcgcg gtggtgctgg
     2761 catctttggt gccgctgtcg atgggcctga tgagcgtgat cgagggcgca agcgtgatcc
     2821 gcggcgtcgg cgcgccgacg acggatctcg atagccactt ccgctacctc tccgggctgc
     2881 tgctcggcat cgggctcgct tatctcgccg cgctgccgcg gatcgagcgc agcacgacct
     2941 tgttccggct gctcggcggc atcatcctgg tcggcgggct cgcccgcttg ctgtcgctgg
     3001 ccgaggacgg cgcgccgagc gaggggcatg ttttcgggct ggcgatggag ctgggcgtcg
     3061 tgccggcgat cacattgtgg caatcgcgca tcgcccgccg cttttccgga ccgggcaaga
     3121 aatgacgatg tgaaaagacc gccggagcca tggccccggc ggtcccttac atggtcagcg
     3181 gccggaagct ccagcccggg agaccattga aaagcttacg ctttcatccc ccgaacgagc
     3241 agaaccgacc cccttgctga accatgagac atcggatcac ctcctttcgc ttgttgaaca
     3301 cggaccgata atgagtcggg ccaagtccgc gatcaagtct tgtattgaac tattttggcg
     3361 ctaatcttcg acccttcgcg ccgcccgtcc ggcgccctcc ccctcagctc cggcaatcct
     3421 cgatcacccg cgccggctcc ggccaggacg ggacgaccag catcggcagc cccgcctgct
     3481 cgaccgtgaa gcggccgcgg ctgaaggcga tcgcatcgag cagcggatcg cgcgcgggga
     3541 gcgccgccga cccgccgctt agcgcgaggc tgcgcgacaa ggtcgaggtg cggatggtga
     3601 tcgcattgcc cggcccgccg ccgccgcgcg cgagcagcac ctggcggccg gcgcggtcgc
     3661 agcggacggt gaaggcgggt ccgccggccg cctggccgaa cgccgccgac gggccggcgc
     3721 cctcggcgcg atagacccag ccgcccggcg tcaagggcac gtcgcgccaa tccgcgatcg
     3781 gcggcggcgg cggcagcgga cgcgccggct gcggctgcgg cggccgcggc tgctgctggg
     3841 gcggagccgg cggcggcggc cggcgctgcg gcgcgcaggc ggcggccccg gccaaaagtc
     3901 caagcaccac ccaatcacgc actcgcctca tcgacatcgg ctccctcatc gcggctgatc
     3961 aaccgcatga gcggcttttc gatcccgcat caagcggcat atcgggagat gggggcggat
     4021 gcggggccgg ggcggcgggg gccggggcgg gggccggtca gccggcgacc agcttgacgg
     4081 aggcggccgg cgcccagccg gagcggcacg gcccttcata ggcgcggcgc gacgcgaccg
     4141 gggcggcgac gccgcagcgc ggatcgagcg cgccggcctc gtcatagacc acccccagcc
     4201 agcgctggtc gtgcgagcgg gtgcagacga agaagcggct ggcggcggcg atgctgccgg
     4261 tctgggcggc atggtcgaac ggcgccgagc gcaccggcag cgtctccccg gcggcgaggt
     4321 tgcgcggcgt tccggccccg ttgcaggcgg cgaagctcgg cccgagctcc ccgatccgga
     4381 ccggcgccgc atcggccgcg gccggcgtgc gggcggtggc ctcgccctcc cggtcgtcgc
     4441 gcagatcctt ggcgaaatga tcgtccttca ccgattcccc gcagccggcg agggcgagag
     4501 cgagcggcag caacagggcg gggaagaggg caaaggagcg catcgccggc atagtggcgc
     4561 gaacggcggg aaaaatctac gcttggcggc gcggccgcgg ccttgcgatg gcgcggcggc
     4621 cgggaagcag gcgaaggagg cggcggcgcg gcatggcgta ttggctgatg aagcggagcc
     4681 cggcagctat ggctgggcgg acctggtgcg cgacggcggc gccgaatggg acggcgtgcg
     4741 caacccggcg gcgcggctcc acctcaaggc gatggcggcg ggcgacgagg ccttcttcta
     4801 ccatagcggc gccgacaagg cggtggtcgg cgtgatgcgc atcgcccgcg ccgccgcgcc
     4861 cgaccccaag gacccggcct gggtctcggt ccgcgtcgag ccgctccgcc cgctcccggc
     4921 cccggtgacg ctgaaggcga tcaaggccga gccggcgctg gcggcgatgg agctgatccg
     4981 ccagtcgcgg ctgtcggtga gcccggtgcg cggcgaggag tgggaggcga ttttgggttt
     5041 gagcgcccga gcatgaagaa gctcccagga gagcgccaaa gccggcgagc ggccgtccgc
     5101 cgccgctcca aagacgacaa gatccgcatg tccgcaagcg catcgcgtca tctgtgtcat
     5161 ctttggccgg ggcggtcggg aattttcccg agccggtcgg cgaggccgtc g
//
LOCUS       DASVRK010000044        10607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_69625, whole genome shotgun sequence.
ACCESSION   DASVRK010000044 DASVRK010000000
VERSION     DASVRK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69625"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VF589_01665"
     CDS             <1..1593
                     /locus_tag="VF589_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEX8256314.1"
                     /translation="GRRTEAMPVVFKSSPGRGGGPPPQAVVEGGLRLAQPPLHQPSAG
                     PPPRSGEDLLPLVTTHRAGQRVEIAAACPAARALGLHPGMAVTQARALVPALDIRDAE
                     PAADAALLHRLGLFAARRWTPKAAVSDLAGLWLDLSGVAHLFGGERAMCARILRFCAR
                     LGLGARIAVAGTPGTAHALARFAPGPITLCPAGAEAEAIAPLPLAALRIGDEVLSAAR
                     RLGLDRVGDLIAMPRGPLNRRFGATLLTRLDQALGRSAEPFDPIMPVEPPAATLRFAE
                     PIATAEAIETALGLLMARLIEALARRSLGARTLALLCARIDGDEQRVAIGTARATRDG
                     GHLTRLLAARIDRIDPGFGIEAMHLVALRTERLEARPIEGELAGDKPAPDLAPLVDRL
                     AGRLGPRRLFRSSAVESDVPERSVRRVGPLAPAARWPDWPRPVRLLAPPERVENVVAL
                     LPDQPPRRFTWRGRSHSVARADGPERIYGEWWRHSGEADAVRDYFQVEDEEGRRFWLY
                     RRGDGVDARTGDLSWHLQGLFG"
     gene            complement(1599..2009)
                     /locus_tag="VF589_01670"
     CDS             complement(1599..2009)
                     /locus_tag="VF589_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8256315.1"
                     /translation="MILIDTHPLIWFSQGDKKLGSKTCRLIEESFAAGACVVSPMSFW
                     EAAMLTDKRRIALGRAVIEWMNEVLRQGVRLAPLSPEIAIDAGQLPGDIHGDPSDRII
                     VATARALSCSLITADHLILAYGEAGHVATIDAKR"
     gene            complement(2006..2296)
                     /locus_tag="VF589_01675"
     CDS             complement(2006..2296)
                     /locus_tag="VF589_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX8256316.1"
                     /translation="MGKMIGATEFKAHCLRILDEVERTGESVTITKRGRIVGELHPPR
                     ENKEPPREKKETKFGFLKHPEYRFEDPFSPVYEGPWNAELDRDEDDPGTGMA"
     gene            2439..5843
                     /locus_tag="VF589_01680"
     CDS             2439..5843
                     /locus_tag="VF589_01680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX8256317.1"
                     /translation="MYVELQATTHFSFLRGVSSAEELFSAAALLGYPALGFTDRNSVA
                     GLVRAMLAAETTGVRLVAGCRLDLIDPCPEQGRRGASLLVWPEDRAGWSRLTRLLTIG
                     KARAEPKKGEKGKCFLHWEDVAAWSDGLVAALIPGEADAATAAALGQLSDIFGSRAHL
                     VLCRRRRPGDALRLHELDALARHYGVRGLAAGDILYDSPDKRLLQDVVTAIRHKCTID
                     DLGFRRERHADRHLKGPGEMERRFAEHPDAVRATADIAERCRFSLRELSYQYPDEIVM
                     SGRSPQQALERLTWDALHAKFPGGIPEAYAKLLTHELGLVAKLGYAPYFLTVNSIVGY
                     ARSQQILCQGRGSAANSMICYILDITSIDPVQHQLLFERFISGERKEPPDIDVDFEHE
                     RREEVIQWIYETYGHRHAALTAVVSRYRTRGAVREVGKALGLPEDLTGALAGQVWGWS
                     NEGVEDRHLAELNLDGRDPRLALTIDLARQLIGTPRHLSQHPGGFVLTRDRLDDLVPI
                     EPAAMADRRVIEWEKDDIEALGFMKVDVLGLGMFGCLRRAFDLLAEHKRIEIGLADLQ
                     EDDPVVYEMIRRADTLGVFQIESRAQMSMLPRMKPKEFYDLVIEVAIVRPGPIQGDMV
                     HPYLRRREGKEKVEYPSKALEAVLWKTLGVPLFQEQAMKVAIVGAGFTAAEADALRRS
                     MATFKFTGGVGAFQSKMIDGMLANGYTRDFATRTFKQIEGFGSYGFPESHAASFAKIA
                     YASSWMKCHHPDVFCAALLNAQPMGFYAPAQILRDAREHGVELRPVCINASRWDCTLE
                     AKTPPRNGEGDHAQHGGGAAQAKPEPYSSLRSAPPPPYGWSPSPANAGEDYLPLRLGL
                     RMVRGLSNDHAARIVAARAEAPFASVEEVWRRSGVPVAALEKVADADGFRSLGLDRRQ
                     ALWRVRGLGETPLPLFAAADARGAEPEVALTPMTGGREVVEDYRAVQLSLRAHPLSFL
                     RAELDRRRIVRCADLARIKDGRKVEVAGIILVRQRPGSTNVTFVTIEDETGIANAIIW
                     QRRFEAQRRTIMSSAMVAIKGTVQREGEVIHVIADHLEDYTPLLHSVGEMHFPHRTGP
                     GDGASHGGAPDPRGDWEKRVRDDYHAPFRTRPDADAVIRQKTRDFH"
     gene            5869..6309
                     /locus_tag="VF589_01685"
     CDS             5869..6309
                     /locus_tag="VF589_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256318.1"
                     /translation="MRIRSILPLSAILLAAPAAAKPPREQSPAPRDVLAEVGLGNSDQ
                     ELARAAAAADAHPLGTPQNPVRVGGPAGERAYLARLRCADGSPPAIGARRSAGVAAFG
                     SVAGLYSLDCGAAAPGKSELVMDMYHDEHVEVRAPAGFRIEPVR"
     gene            complement(6306..7106)
                     /locus_tag="VF589_01690"
     CDS             complement(6306..7106)
                     /locus_tag="VF589_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX8256319.1"
                     /translation="MIWSDGHLRSYFERIGYDGVPAPDLATLAGLQRAHRLAIPFENL
                     DIPIGRGIELAPERLFDKLVTRRRGGYCFEQNSLFLGLLRALGYEARPLLARVWLMAE
                     ETPPLTHTLNLVRMGGADFVADVGFGGGYAPPLRLAEDEPVTTPDGVRHRLVRDGEHG
                     WMLERDAGPGWTRQYSFAEAFVWPVDLEVANHFTATRPNTRFTSLRIVSMTLPDGFAS
                     LLDRALTVSRGEAREVSEVGDAESYRRILAERFGLELAADEVRRLGLF"
     gene            7187..8266
                     /locus_tag="VF589_01695"
     CDS             7187..8266
                     /locus_tag="VF589_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8256320.1"
                     /translation="MRKLLKGIGTALLFLAIAICLAPTLVPPFLDRIYYQGPRSDHFD
                     GERFFNPGFGAYRGGNPSTFLNRWANGERATWPERVSIRQSAPPARIAGPEMRVTWVG
                     HATVLVQTQGLNILTDPVWSDRVSPLPFIGPVRVREPGVAFDRLPKIDLVLISHNHYD
                     HMDLPTLMRLWQRDRPLIVTSLGNDTILSGQGIAATALDWGGRVPVRPGIEAVVERNH
                     HWGSRWGTDRNRALWSAFTIVLPGGNSFYAGDTGWGDGGWVREAAASGPFRLAIIPIG
                     AYEPRDFMKTNHINPEEAVRIFETLNPAQALGIHWGTFQLTFEPIDEPRHKLEALKQA
                     RGIAPARFIATEAGASFSVPALPAR"
     gene            complement(8238..8558)
                     /locus_tag="VF589_01700"
     CDS             complement(8238..8558)
                     /locus_tag="VF589_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX8256321.1"
                     /translation="MASGPYAIIWRYEVEPAHREAFEAGYAPAGEWARLFAVAPGFIR
                     TELMAGGDSCYVTIDYWRCEADFRAFQAKSGAEYRALDRTCEPWTRKEERIGAFTVLE
                     APER"
     gene            complement(8577..9107)
                     /locus_tag="VF589_01705"
     CDS             complement(8577..9107)
                     /locus_tag="VF589_01705"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HEX8256322.1"
                     /translation="MNYPLLAAAAALLSLAACNRSPQVDEVDMTADEVAEQLAEMKIK
                     PGQWEATNEIISASAPGLPPETLKQMVGQKTTVKNCITPEQAAKPNHNFLAVQQNSEC
                     TYQDWAMEGGRMTGTMTCSGGEMPGNVVMTMDGKYGETAYDLNMNMETSGLPGGMTMN
                     IQARTTGRHVGECTGS"
     gene            complement(9118..10458)
                     /locus_tag="VF589_01710"
     CDS             complement(9118..10458)
                     /locus_tag="VF589_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8256323.1"
                     /translation="MRLVLIALAAALAAAPAAAQDLSSAVKADYDKRLGALFVDFHKN
                     PELSYVEKRTARIMAKELRAAGATVTEGVGGTGVVGVMKNGAGPTVLVRADMDGLPLK
                     EASGLAYASAARQVGVDGVEAPVMHACGHDVHITSLVGTARRLAAMKDRWKGTVLFVV
                     QPAEERIGGARKMLEDGLYTRFPKPDYALAFHVDADSPTGRIIADPGITASSSDSVDI
                     IVHGVGTHGAYPHQGKDPIVMGAEIIMALQTLVSREVSPLKPAVVTVGAFHAGLKHNI
                     ISDRAELQLTVRSDHADVRKTLLDGIRRIAVNVGRMNGLPETKLPEVKLSTESTPATY
                     NDAALTERMRRVFTGAFGPNVLISPERSGMGAEDFAYFVQPELGVPGFYFSVGGTPQA
                     AFDAAEAGGPPVPAHHSPLFKIEPEPSVRLGTEAMTVAVLDLLLPNTAARAAGH"
BASE COUNT         1550 a         3957 c         3414 g         1686 t
ORIGIN      
        1 ggccgccgca ccgaagccat gccggtcgtg ttcaaatcct ccccgggacg gggaggggga
       61 ccgccgccgc aggcggtggt ggaggggggc ctccgcctag cacagccccc cctccaccag
      121 ccttcggctg gtccccctcc ccgttccggg gaggatttgc tccccttggt caccactcac
      181 cgcgccggcc agcgggtcga gatcgccgcc gcctgcccgg ccgcgcgggc gctcggcctc
      241 caccccggca tggccgtcac ccaggcgcgc gcgctcgtcc ccgccctcga cattcgcgat
      301 gccgagcccg ccgccgacgc cgccttgctc caccggctcg gcctgttcgc ggcgcgccgc
      361 tggacgccca aggccgccgt ctcggacctg gccggcctct ggctcgacct cagcggggtc
      421 gcccatctgt tcggcggcga gcgggcgatg tgcgcccgta tcctccgctt ctgcgcccgg
      481 ctcgggcttg gcgcccgcat cgccgtcgcc ggaacgcccg gcaccgctca cgctctggcg
      541 cgcttcgccc cgggcccgat caccctctgc ccggcgggcg cggaggccga ggccatcgcc
      601 cctctgccgc tggccgcgct ccgcatcggg gacgaggtgc tgagcgccgc ccgccgcctc
      661 ggcctcgacc gcgtcggcga tctcatcgcc atgccgcgcg gcccgctcaa ccgccgtttc
      721 ggcgccactt tgctcacccg gctcgatcag gcgctcggcc gctccgccga gccgttcgat
      781 ccgatcatgc cggtcgaacc gccggccgcc acgcttcgct tcgccgagcc gatcgccacc
      841 gccgaagcga tcgaaaccgc gctcggcctg ctgatggcgc ggctgatcga ggcgctggcc
      901 cggcgatcgc tcggcgcgcg caccctcgcc ctcctttgcg cccgcatcga tggcgacgag
      961 caaagggtcg cgatcggcac cgcccgcgcc acccgcgacg gcggccatct cacccggctc
     1021 ctggccgcga ggatcgaccg gatcgacccc gggttcggca tcgaggcgat gcacctcgtc
     1081 gcgctccgca ccgaacggct cgaagcccgg ccgatcgaag gcgaactggc cggggacaag
     1141 cccgcgcccg acctcgcgcc tttggtcgac cgcctcgccg gccgcctcgg cccccgccgc
     1201 ctgtttcggt cgagcgcggt cgaaagcgac gtgcccgagc gcagcgtccg ccgcgtgggc
     1261 ccgctcgccc ccgccgcgcg ctggcccgat tggccgcgcc ccgtccgcct gctggccccg
     1321 cccgaacggg tcgagaatgt cgtcgccctg ctgcccgatc agccgccccg ccgcttcacc
     1381 tggcgcggcc ggtcgcactc ggtcgcccgc gccgacggcc ccgagcgcat ctatggcgaa
     1441 tggtggcgcc acagcggcga ggccgacgcg gtgcgcgatt atttccaggt cgaggatgag
     1501 gagggccgcc gcttctggct ctaccgccgc ggcgacggcg tcgacgcccg caccggcgac
     1561 ctcagctggc acctgcaggg gctgttcgga tgagggggct aacgcttggc gtctatcgtg
     1621 gcgacatgcc cggcctcgcc ataggccaga atcaggtgat cggccgtgat cagcgaacag
     1681 ctcaatgctc tggccgtcgc cacgatgatc cggtcggaag gatcgccgtg gatgtcaccc
     1741 ggcagctgcc cggcatctat cgcaatctcg ggcgaaaggg gagcaagcct gaccccttgg
     1801 cgaagcactt cattcatcca ctcgatgacg gctcgtccca atgcaatgcg ccttttgtcc
     1861 gtcagcatcg ccgcttccca aaagctcatc ggcgaaacca cgcatgcacc tgcggcaaag
     1921 ctttcctcaa taagccggca ggtcttgctc cccagcttct tgtcgccctg gctgaaccag
     1981 atcagcggat gtgtatcgat caggatcacg ccattccggt tcccggatcg tcctcatccc
     2041 tgtcgagttc cgcattccat ggcccttcat atacagggct gaacgggtcc tcgaaccgat
     2101 attccgggtg cttgaggaaa ccgaatttgg tttccttctt ctcgcgcggt ggttccttat
     2161 tttcgcgcgg cggatggagt tcgcccacga tccggccgcg cttggtaatg gtgacagact
     2221 ccccggtgcg ctcaacctcg tcgaggatgc gcaggcagtg cgccttgaac tcggtcgcgc
     2281 cgatcatctt gcccatgtcg gcctctcctc aactggtcac ttcatatagt cactttccca
     2341 gcgctccgca aatcgggagg agaaaacgga cgacattggc aagcctcaac ttcctcaact
     2401 tattcgcccg cgcggcgccg gccggggccg cctgagccat gtatgtcgag cttcaggcca
     2461 ccacccattt cagcttcctg cgcggcgtct ccagcgcgga ggaattgttc tcggccgcgg
     2521 ctttgctcgg ctatccggcg ctcggcttca ccgaccgtaa ttcggtcgcc ggcctcgtcc
     2581 gcgccatgct cgcggccgag acgaccggcg tgcgcctcgt cgccggctgc cggctcgacc
     2641 tcatcgaccc ctgccctgag caaggtcgaa ggggcgcctc tttgctggtc tggccggagg
     2701 accgcgccgg ctggtcgcgg ctcacgcggc tgctcaccat cggcaaggcc cgcgcggagc
     2761 cgaaaaaggg cgagaagggc aaatgcttcc tccactggga ggatgtcgcc gcctggagcg
     2821 acgggctcgt cgccgcgctc attccgggcg aggccgacgc cgccaccgcg gccgcgcttg
     2881 ggcagctcag cgacattttc ggctcgcgcg cccatctcgt gctctgccgc cgccgccgcc
     2941 ctggcgacgc gctccgcctc cacgaactgg acgcgctcgc ccggcattat ggcgttcgcg
     3001 gcctcgccgc cggcgacata ctctacgatt cgcccgataa gcgcctgctc caggacgtgg
     3061 tcaccgccat ccgccacaaa tgcaccatcg acgatctcgg cttccgccgc gaacgtcatg
     3121 ccgaccgcca cctcaagggg cccggcgaga tggagcggcg cttcgccgaa catcccgatg
     3181 cggtccgcgc caccgccgac atcgccgagc gctgccgctt ctccctgcgc gagctcagct
     3241 accaatatcc cgacgagatc gtgatgtcgg gccgcagccc gcagcaggcg ctggagcggc
     3301 tcacctggga cgcgcttcac gccaaattcc ccggcggcat ccccgaagct tatgcgaagc
     3361 tcctgaccca cgagctgggg ctggtcgcga agctcggcta cgcgccttat ttcctcaccg
     3421 tcaattcgat cgtcggctat gcgcgcagcc agcagatcct ctgccagggc cgcggatcgg
     3481 ccgccaattc gatgatctgc tacatcctcg acatcacctc gatcgatccg gtgcagcacc
     3541 aattgctgtt cgagcgcttc atctcgggcg agcgcaagga gccgcccgac atcgacgtcg
     3601 atttcgagca tgagcggcgc gaggaagtga tccagtggat ctacgagacc tacggccacc
     3661 gccacgccgc tttgaccgcc gtcgtcagcc gctaccgcac ccgcggcgcg gtgcgcgagg
     3721 tcggcaaggc gctcggcttg cccgaggatc tgaccggggc gctggccggc caggtctggg
     3781 gctggtcgaa cgagggggtg gaggaccgcc acctcgccga actgaacctc gacggccgcg
     3841 acccgcgcct cgccctcacc atcgacctcg cccgccagct gatcggcacg cctcggcatc
     3901 tcagccagca tcccggcggc ttcgttctca cccgcgaccg cctcgacgat ctcgtcccga
     3961 tcgagccggc ggcgatggcg gaccgccgcg tcatcgaatg ggagaaggac gatatcgaag
     4021 cgctcggctt catgaaggtc gacgtgcttg ggctcggcat gttcggctgc ctgcgccgcg
     4081 ccttcgatct cctggccgaa cataagcgca tcgaaatcgg cctggccgac ctccaggagg
     4141 acgatcctgt cgtctacgag atgatccggc gcgccgacac tttgggcgtg ttccagatcg
     4201 aaagccgcgc ccagatgtcg atgctgccgc ggatgaagcc gaaggaattt tacgacctcg
     4261 tcatcgaggt ggcgatcgtc cgccccggcc cgatccaggg cgacatggtc cacccctatc
     4321 tgcgccggcg cgagggcaag gagaaggtcg aatatccttc gaaggcgctc gaggcggtgc
     4381 tgtggaagac attgggcgtg ccgctgttcc aggagcaggc gatgaaggtg gcgatcgtcg
     4441 gcgccggctt caccgcggcc gaggccgacg cgctgcgccg ctcgatggcc accttcaagt
     4501 tcaccggcgg cgtcggcgcc ttccagagca agatgatcga tggcatgctc gccaacggct
     4561 atacgcgcga cttcgcgaca cgcaccttca agcagatcga gggcttcggc agctacggct
     4621 tccccgaaag ccacgcggcc agcttcgcca agatcgccta cgcctcgtcc tggatgaaat
     4681 gccaccatcc cgacgtcttc tgcgcggctt tattgaacgc ccagccgatg ggcttctacg
     4741 cccccgccca gatcctccgc gacgcccgcg agcacggcgt cgagctccgc ccggtctgca
     4801 tcaacgccag ccgctgggat tgcacactgg aagctaaaac tcctccccgg aacggggagg
     4861 gggaccatgc gcagcatggt ggaggggcgg cgcaggcgaa gccggagccc tattcctcgc
     4921 ttcgctcggc ccctccccca ccctacgggt ggtccccctc ccctgcaaat gcaggggagg
     4981 attacctccc cctccgcctc gggctccgca tggtgcgggg cctctcgaac gaccatgccg
     5041 cccgcatcgt cgccgcccgc gccgaggcgc ccttcgcttc ggtcgaggag gtctggcgcc
     5101 gctcgggcgt gccggtcgcc gcgctcgaga aggttgccga tgccgacggc ttccgctcgc
     5161 tcgggctcga ccggcgccag gcgctgtggc gggtgcgcgg gctcggcgag acgccgctgc
     5221 ccctgttcgc cgccgccgac gcgcgcggcg ccgagccgga agtggcgctg accccgatga
     5281 ccggcggccg cgaagtggtc gaggattatc gggcggtcca gctgtcgctg cgcgcccatc
     5341 cgctgagctt cctgcgcgcc gagctcgacc gccgccgcat cgtccgctgc gccgacctcg
     5401 cccgcatcaa ggacggccgc aaggtcgaag tcgccggcat catcctcgtc cgccagcggc
     5461 cgggctcgac caacgtcacc ttcgtcacga tcgaggacga gaccggcatc gccaacgcga
     5521 tcatctggca gcgccgcttc gaggcgcagc gccgcaccat catgtcctcc gcgatggtcg
     5581 ccatcaaagg cacggtgcag cgcgaaggcg aagtgatcca cgtcatcgcc gaccacctcg
     5641 aagattatac gcctctgctc cactcggtcg gcgaaatgca tttcccgcac cgcaccggcc
     5701 cgggcgatgg cgcttcccat ggcggcgcgc ccgatccgcg cggcgattgg gagaaaaggg
     5761 tccgcgacga ttatcacgcc cccttccgga cccggcccga cgccgacgcc gtcatccgcc
     5821 agaagacgcg ggattttcat tagccgccgg gaccgctagg agcgccgcat gcgtatccgt
     5881 tcgatcctcc cgctctccgc gatcctcctc gccgcgcccg ccgccgccaa gccgccgcgc
     5941 gaacaatccc ccgccccgcg cgacgtgctg gccgaggtcg gcctcggcaa tagcgatcag
     6001 gagctggccc gcgccgccgc cgccgccgac gcccatccgc tcggcacccc ccagaatccc
     6061 gtccgggtcg gcggcccagc cggcgagcgc gcctatctcg cccggctgcg ctgcgccgac
     6121 ggctcgcccc ccgccatcgg cgcccgccgc tccgccggcg tcgccgcttt cggctcggtc
     6181 gccggcctct attcgctcga ttgcggcgcg gccgcccccg gcaagagcga gctcgttatg
     6241 gacatgtatc atgacgagca tgtcgaggtt cgcgccccgg ccggcttccg gatcgagccc
     6301 gtccgctaga acaagccgag ccgccgcacc tcgtccgccg ccagctcgag cccgaaccgt
     6361 tcggccagga tccgccggta gctctccgca tccccgactt cgctgacttc ccgcgcctcg
     6421 ccgcgactga cggtgagcgc gcggtccagc agcgaggcaa agccgtccgg cagcgtcatc
     6481 gagacgatcc gcaagctggt gaagcgcgta ttcgggcggg tggcggtgaa gtggttcgcg
     6541 acttccaggt cgaccggcca gacgaaagcc tcggcgaagc tgtattgccg cgtccagccg
     6601 gggccggcgt cccgctccag catccagcca tgctccccgt cccgcaccag ccggtgcctg
     6661 accccgtccg gagtggtgac gggttcgtcc tcggccagcc gcaagggcgg cgcgtagccg
     6721 ccgccgaagc ccacgtcggc gacaaagtcg gcgccgccca tccggaccag gttgagggtg
     6781 tgggtgagcg gaggcgtctc ctcggccatc agccagacgc gggccagcag cggccgcgcc
     6841 tcgtagccga gcgcgcgcag cagccccagg aacaggctgt tctgttcgaa gcaatagccg
     6901 ccgcgccggc gcgtcacgag cttgtcgaac agccgctccg gcgccaactc gatgccgcgg
     6961 ccgatcggta tgtccagatt ctcgaacggg atggccagcc ggtgggcgcg ctgcaggccg
     7021 gccagcgtgg caaggtccgg cgccggaacg ccgtcatatc cgatccgctc gaaataggat
     7081 cgaagatgac cgtcgctcca gatcatccgt cactgctcgc cgaaaccggg gccgagatca
     7141 acgcaagatc ggttgcccgc cctccgcgcg ccgctatgag gcgcggatgc gcaagctgct
     7201 gaaggggatc gggacggccc tgctgttcct cgcgatcgca atctgcctgg cgccgaccct
     7261 ggtgccgccg ttcctcgacc gcatctacta tcagggcccg cgctccgacc atttcgacgg
     7321 cgagcgcttc ttcaatcccg gcttcggcgc ctatcgcggc ggcaatccca gcaccttcct
     7381 caatcgctgg gccaatggcg agcgcgccac atggccggag cgggtgtcga tccggcagag
     7441 cgctccgccc gcccgcatcg ctgggccgga gatgcgcgtc acctgggtcg gccacgccac
     7501 cgtgctcgtc cagacccagg ggctcaacat cctcaccgat ccggtctggt ccgaccgcgt
     7561 ctcgccgctg ccgttcatcg gcccggtgcg ggtgcgcgaa ccgggcgtcg ccttcgatcg
     7621 cctgccgaag atcgacctcg tcctcatcag ccacaaccac tacgaccata tggacctgcc
     7681 gaccctgatg cggctgtggc agcgggaccg gccgctgatc gtcaccagcc tcggcaacga
     7741 caccatcctc tcgggccagg gcatcgcggc gacggcactc gactggggcg gccgcgtgcc
     7801 ggtccggccc ggcatcgaag ccgtggtcga gcgcaaccat cattggggct cgcgctgggg
     7861 caccgatcgc aaccgcgcgc tctggtcggc cttcaccatc gtgctgccgg gcggcaacag
     7921 cttctacgcc ggcgacaccg gctggggcga cggcggctgg gtgcgagagg ccgccgccag
     7981 cggcccgttc cgcctcgcga tcatcccgat cggcgcctac gagccgcgcg acttcatgaa
     8041 gaccaaccac atcaacccgg aggaggcggt gcggatcttc gagacgctga acccggccca
     8101 ggcgctcggc atccactggg gcaccttcca gctcaccttc gagccgatcg acgagccgcg
     8161 ccacaagctc gaagcgctga agcaggcgcg cggcattgcc ccggcccgct tcatcgccac
     8221 cgaagcgggc gccagcttca gcgttccggc gcttccagca cggtgaaggc gccgatccgc
     8281 tcctccttgc gcgtccaagg ctcgcatgtc cggtcgagcg cccgatattc cgcgcccgat
     8341 ttcgcctgga acgcgcggaa gtccgcctcg caccgccaat aatcgatcgt cacgtagcag
     8401 ctgtcgccgc ccgccatcag ctcggtgcgg ataaagcccg gggcgacggc gaacagccgc
     8461 gcccattcgc ccgccggggc atagccggcc tcgaacgcct cgcgatgcgc cggctcgacc
     8521 tcgtagcgcc agatgatggc atagggcccg gacgccatcg cttcagaggc tccggctcag
     8581 gagccggtgc attcgccgac atggcggccg gtggtgcgcg cctggatgtt catggtcatg
     8641 ccgccgggca ggccgctcgt ttccatgttc atgttgaggt cgtaggcggt ctcgccatat
     8701 ttgccgtcca tggtcatcac cacgttgccc ggcatctcgc cgcccgagca ggtcatggtg
     8761 ccggtcatgc ggccgccctc catcgcccaa tcctgatagg tgcactcgct attctgctgc
     8821 accgccagga aattgtggtt gggcttggcc gcctgctcgg gcgtgatgca gttcttcacg
     8881 gtcgtcttct gcccgaccat ctgcttcagc gtctcgggcg gcagccccgg cgcgctcgcc
     8941 gagatgatct cgttggtcgc ctcccattgg ccgggcttga tcttcatctc ggccagttgc
     9001 tccgcgacct cgtcggcggt catgtcgacc tcatcgacct ggggcgagcg gttgcacgcg
     9061 gccagcgaca gcagggcggc ggcggcggcg agaagggggt agttcatcgg gggctcctta
     9121 gtggccggcg gcgcgcgcgg cggtattggg cagcaacaaa tcgaggacgg cgacggtcat
     9181 cgcttcggtg ccgagccgca ccgacggctc cggctcgatc ttgaacaagg gcgaatggtg
     9241 cgcgggcacc ggcggcccgc ccgcttcggc ggcgtcgaac gccgcctgcg gggtgccgcc
     9301 gacgctgaag tagaaccccg gcacgccgag ctcgggctgg acgaaataag cgaaatcctc
     9361 cgcccccatg ccgctccgct cgggcgagat cagcacgttc ggcccgaatg cgccggtgaa
     9421 cacccgccgc atccgctcgg tcagcgctgc atcgttatag gtggcgggcg tcgattcggt
     9481 ggagagcttc acctccggca gcttggtctc gggcaggccg ttcatccgcc ccacattgac
     9541 cgcgatgcgc cggatcccgt cgagcagggt cttgcgcacg tcggcatggt cggagcggac
     9601 ggtgagctgc agctcggccc ggtccgagat gatgttgtgc ttgagcccgg catggaaggc
     9661 gccgaccgtc accaccgccg gcttcagcgg cgatacctcc cggctgacca gggtctgcag
     9721 cgccatgatg atctcggcgc ccatcacgat cgggtccttg ccctggtgcg gataggcgcc
     9781 gtgcgtgccg acgccgtgga cgatgatgtc gacgctgtcg gaggaggaag cggtgatgcc
     9841 gggatcggcg atgatccggc cggtcggcga atccgcgtcg acatggaagg cgagcgcgta
     9901 atcgggcttg ggaaagcgcg tgtagagccc gtcctccagc atcttgcggg cgccgccgat
     9961 gcgctcctcg gccggctgca ccacgaacag caccgtgccc ttccagcggt ccttcatcgc
    10021 cgccagccgg cgggcggtgc cgaccagcga ggtgatgtgc acgtcgtgcc cgcaggcgtg
    10081 catcaccggc gcctcgacgc cgtccacgcc cacctgccgc gcggccgagg cataagccag
    10141 gccgctcgcc tccttgagcg gcaggccgtc catgtcggcg cggaccagca cggtcggccc
    10201 ggcgccgttc ttcatcacgc cgacgacgcc ggtcccgccg acgccctcgg tcaccgtcgc
    10261 tccggcggcg cgcagctcct tggccatgat ccgcgcggtg cgcttctcga cgtagctcag
    10321 ctccggattc ttgtggaagt cgacgaacaa agcgccgagc ctcttgtcgt aatcggcctt
    10381 gaccgcgctc gacagatcct gcgcggccgc gggcgcggcc gccagcgccg cggccaaagc
    10441 gatcagtacc agacgcatag gtccccctct gttgctcatc cgcccgtgtc tagcgcggcg
    10501 gccgcatatt gccagtggag cttcaggccg tgcaggcgcc gagccggcgg gcggtgagct
    10561 tcgaggatat cttcacgtcg ccgtcgctgg cgagataggt gcgggcg
//
LOCUS       DASVRK010000045         6146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_566881, whole genome shotgun sequence.
ACCESSION   DASVRK010000045 DASVRK010000000
VERSION     DASVRK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566881"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VF589_01715"
     CDS             <1..701
                     /locus_tag="VF589_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8256324.1"
                     /translation="TINLNVLDRAHKRNIHRCFYSSSACMYPAYNQEDPDNPNCEESS
                     AYPAAPDSEYGWEKLFSERLYLAYNRNYGMTNRVARYHNIFGPEGTWQGGKEKAPAAV
                     CRKVATAMNNGEIEIWGDGTQTRSFLYIDECVEGSIRLTRSEFEGPVNIGSEEMVTIN
                     QLVDIVCDIAGKTLHKNHIEGPTGVRGRNSDNRLIEEKLGWKPSQSLTTGLERTYEWI
                     ELQVRRNAPTARAA"
     gene            864..2072
                     /locus_tag="VF589_01720"
     CDS             864..2072
                     /locus_tag="VF589_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256325.1"
                     /translation="MMHSRLALPAAMALTLTACGADANAAAVPAKPGQDNRPALQAAI
                     DRLSAAGGGTLQLENGTYDVDGPIYVRSGVTIAGRGRGTIITNQKFRQGTGWGGITVF
                     AGNLNPGSFSNALNEGYPPRPTRRVSDRELTIDNCNAREVAGLTGRVIWLSTELSEPG
                     RRGRPNPRYGEMSLVERIDGCRVRLADPISAPANVPVRIHWSDGSRALPLDEPNVPIR
                     DAAMRDLQLASPASQALLVSGCYRCTFTNIHIGESRRLIALQGTRRNRFTGITGTFTE
                     RGIEAVQYATDNIVENVDAEYRPGASERVRPAIRFGEYARDNVVRNVQLRLGRDYIGR
                     DKIRFDESAGNRLVNIKLILPTSDNRRSVVYRAPDSQRDSERQLPPGTSLQGVQLCFG
                     QNGGTCEPIQ"
     gene            2056..3237
                     /locus_tag="VF589_01725"
     CDS             2056..3237
                     /locus_tag="VF589_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX8256326.1"
                     /translation="MNRSSDASGTGAGEARAARPLTILGAYGGVNSGDEMILRAVVRQ
                     ARRAGYRGPINVVAGHVPAGASLRADYLDEQLVVHSWRDRAKALRAAAGRDLFIGGGQ
                     LIDGAFGINHPLVQTALAMVARATGGKVAIGGVSTARLESPKVRMLYRLLFGLAGRIA
                     ARDEASLADILAIAPSAATKSERLADMVFALADELGGGRPVEERRALVFAVHHAPHLP
                     LTDMDEAAGFLGRAAALAGEQVELKVVAHDHRPEWDLDFARRLAARAGLPGDAVRTLA
                     TTAECADFYRAARSVVSVRMHPIILGACAGAYCVPVKGSRKQADLAGRLGLPSWSLAE
                     MMALDDMAFGAAVGIGAGGPRPDRARLERLSEEARAVLAPAAGAGARQSAPAQAAAGR
                     A"
     gene            3283..4269
                     /locus_tag="VF589_01730"
     CDS             3283..4269
                     /locus_tag="VF589_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8256327.1"
                     /translation="MRRVLVTGGSGFIGTHLVAGLLSRGDQVSNIDLKPPILAEQTGC
                     WTKLDLMDHEGLARHVAEFRPHVVYNLAAVADIALGGEALKVNTEGLRHLLEAAQALP
                     EPPRLIHASTQLVAGPGYQISGPRDYSPYTEYGESKAASEEILWNWTGDIVWTIVRPT
                     VVWGPWHPSFGGSTWKYLNRRWYMITGGKDPVRTYSYVDNLVAQLIAAAEAAPERVGA
                     KMFYGGDAPIPTSWWLDGFAKALTGKPARRVPYALLSGLAWAGEAMGRIGGPSPINRG
                     RLYRMTSEYVVPLDDTFEILGEGPVTLDEGIARTVDWLRADQPEEFGRTRRA"
     gene            complement(4266..5402)
                     /locus_tag="VF589_01735"
     CDS             complement(4266..5402)
                     /locus_tag="VF589_01735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8256328.1"
                     /translation="MKILYVVQNFRQGWGGGPESVRLMANLLAPRGISADVFDLGRFR
                     RNIETLELLPEPGRAAEPFPLDTAPDYDAILIVGPWQNAAQLGPLLKRRPDGQQMLYL
                     PRGGLGRIEFSRLRDLKKIPYFHLIERRLLDRSGGIVFSSEVERDHSIAAARRRAPEW
                     VIPDFFDSPDLTAPAAAQAAERAEGAGEGDRPLTLSFLAEISPRKGLLPLVEGFAAWA
                     AARPSGSRPVRLVVGGAPRAGSERYLAAVHAVQRRHAGRADIEFVGPVAHGDRQRFYA
                     DTDVMVVSSLFESYGLTVLEALSAGCATLAAPDLGVLEYLPAYSRLTIARGAAAADFE
                     AALGPAAASAEAGGREATRSYAAAAIAAINARALAAWTELLGGT"
     gene            complement(5745..>6146)
                     /locus_tag="VF589_01740"
     CDS             complement(5745..>6146)
                     /locus_tag="VF589_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8256329.1"
                     /translation="ESLEHSLTAMLLVAVGAALPLLWPYFEPSHAAIGLALVFLIRPL
                     VGWMSLAGTALRGRERLVVSFYGVRGIGSVYYLAYAGHHVELVNEYQLWATVAFTILL
                     STLVHGLTAGLAVERATGEPLTGEEPARETD"
BASE COUNT         1019 a         2052 c         2214 g          861 t
ORIGIN      
        1 cgacgatcaa tttgaacgtc ctcgaccgcg cccataagcg caacatccac cgctgcttct
       61 attcctcgtc ggcctgcatg tacccggcct acaatcagga agaccctgac aatcccaatt
      121 gcgaggagtc gtcggcctat ccggcggcgc cggacagcga atatggctgg gagaaactgt
      181 tctccgagcg gctctacctc gcctataacc gcaattacgg catgaccaac cgggtcgcgc
      241 gctaccacaa tatcttcggc cccgaaggca cctggcaggg cggcaaggaa aaggcgccgg
      301 ccgccgtctg ccgcaaggtg gcgaccgcga tgaacaatgg cgagatcgag atctggggcg
      361 acggcaccca gacccgctcc ttcctctata tcgacgaatg cgtcgaaggt tcgatccggc
      421 tgacccggtc ggagttcgaa gggccggtca atatcggctc cgaggagatg gtgacgatca
      481 accagctggt cgacatcgtc tgcgacattg ccggcaagac gctccacaag aaccatatcg
      541 agggcccgac cggcgtgcgc ggccgcaatt cggacaaccg gctgatcgag gagaagctcg
      601 gctggaagcc gtcccagtcg ctgaccaccg ggctcgagcg cacctatgag tggatcgagc
      661 tccaggtccg gcgcaacgct ccgacggcgc gggcggcctg agcgggctcg catcgagcct
      721 tggtgagtcg cggcggatgt ggcacggggg accggcgccg cagccccgaa tgggcgaagc
      781 ggcagttgtg cagggttcac cgtcggggga ctatgccagt cacgcccggc acgcgggccc
      841 ctcagttcgg aagtgagacg cgcatgatgc attcgagact cgccctgccc gccgcgatgg
      901 cgctgacgct gaccgcgtgc ggcgccgacg ccaacgccgc cgctgtgccg gccaagccgg
      961 gccaggacaa tcgcccggcg ctgcaggcgg cgatcgaccg gttgtcggcg gcgggcggcg
     1021 gcacgctgca gctcgagaac ggcacctacg acgtcgacgg gccgatctat gtccgctccg
     1081 gcgtcaccat cgccggccgc gggcgcggga cgatcatcac caaccagaaa ttccgccagg
     1141 gcaccggctg gggcggcatc acagtgttcg ccggcaatct caacccgggc agcttctcca
     1201 acgcgctcaa cgaaggctat ccgccgcggc cgactcgccg cgtttcggac cgcgagctga
     1261 ccatcgacaa ttgcaatgcg cgcgaagtcg ccggcctcac cggccgcgtg atctggctat
     1321 cgaccgaatt gtcggagccc ggccggcgcg gccgccccaa cccgcgctac ggcgaaatgt
     1381 cgctggtcga gcggatcgac ggatgccgcg tgcggctggc ggacccgatc agcgcgcccg
     1441 ccaacgtgcc cgtccggatc cactggagcg acggcagccg ggcgctgccg ctcgacgagc
     1501 ccaacgtgcc gatccgggac gcggccatgc gcgacctgca gctcgcctcg ccggccagcc
     1561 aggcgctgct cgtcagtggc tgttatcgct gcaccttcac caacatccac atcggcgaat
     1621 cgcggcggct gatcgcgctc caggggacgc ggcgcaaccg cttcaccggg atcaccggaa
     1681 ccttcaccga gcgcggcatc gaagccgtcc aatatgccac cgacaatatc gtcgagaatg
     1741 tcgatgcgga atatcggccc ggggcgagcg agcgggtgag gcccgccatc cggttcggcg
     1801 aatatgcccg cgacaatgtg gtcagaaacg tccagctgcg gctcggccgg gattatatcg
     1861 gccgggacaa gatccggttc gacgaatcgg ccggcaatcg gctggtcaat atcaagctga
     1921 tcctgccgac cagcgacaac cggcgttcgg tggtctatcg cgcgcccgat tcccagcgag
     1981 acagcgaaag gcagctgccg ccggggacat cgctgcaggg cgtccagctc tgcttcggcc
     2041 agaatggcgg gacgtgtgaa ccgatccagt gatgcttccg gaacgggggc tggggaggcg
     2101 cgggcggcac ggccgctgac cattctcggc gcctatggcg gcgtcaacag cggcgacgag
     2161 atgatcctgc gcgcggtggt gcgccaggcg cgccgggccg gctatcgcgg gccaatcaac
     2221 gtcgtcgccg gccatgttcc ggccggagcg tcgctccgcg ccgactatct ggatgagcaa
     2281 ctcgtcgtcc attcgtggcg tgatcgggcg aaggcgctgc gggcggcggc ggggcgggac
     2341 ctgttcatcg gcggcggcca gctgatcgac ggcgcgttcg ggatcaacca tccgctggtg
     2401 cagaccgcgc tcgccatggt cgcccgcgcc accggcggaa aagtcgcgat cgggggcgtg
     2461 agcacggccc ggctcgaaag ccccaaggtg cggatgctgt accgcctgct gttcggcctg
     2521 gcggggcgga tcgcggcccg ggacgaggcg tcgctggccg acatcctcgc tatcgccccg
     2581 tcggcggcga cgaagagcga gcggctcgcc gacatggtgt tcgcgctggc cgacgagctt
     2641 ggcggcggac ggccggtgga ggaaaggcgg gctttggtgt tcgcggtcca ccatgcgccg
     2701 cacctgccgc tgacggacat ggacgaggcg gcgggcttcc tcgggcgggc cgcggcgctg
     2761 gccggggagc aggttgagct caaggtggtc gcgcacgacc acaggccgga atgggacctc
     2821 gatttcgcgc ggcggctggc ggcgcgggcc gggctgccgg gcgatgcggt gcggacgctg
     2881 gcgacgaccg ccgaatgcgc cgacttctac cgggcggcgc ggagcgtggt cagcgtgcgc
     2941 atgcacccga tcatcctcgg cgcctgcgcc ggcgcttatt gcgtaccggt gaagggcagc
     3001 cgcaagcagg ccgatctggc cgggcggctc gggctgccga gctggagcct ggccgagatg
     3061 atggcgctgg acgatatggc tttcggagcg gcggtcggga tcggagcggg cgggccgcgg
     3121 ccggaccggg cgaggctgga gcggctgagc gaggaggcgc gggcggtgct ggcgccggcg
     3181 gcgggcgcgg gggcgaggca gtccgcgccg gcgcaagcgg cggcgggacg ggcctgattt
     3241 ttcgaggttg agcgcccgga ccgggcgagt tgggggaagc gaatgcggcg tgttctagtc
     3301 accgggggat cggggttcat cggcacccat ctcgtcgccg gactgctgag ccgcggcgat
     3361 caggtcagca atatcgacct gaagccgccg atcctggccg agcagacggg atgctggacg
     3421 aagctcgacc tgatggacca tgaggggctg gcgcggcacg tcgccgagtt caggccgcac
     3481 gtcgtctaca atctcgcggc ggtggccgac atcgcgctcg gcggcgaggc gctcaaggtc
     3541 aataccgagg ggctgcgcca tttgctggag gcggcgcagg cgctgccgga gccgccgcgt
     3601 ctgatccacg cctcgaccca gttggtggcg gggcccggct accagatatc cgggccgcgc
     3661 gattattcgc cttacaccga atatggcgag tcgaaggccg cttcggagga gatactctgg
     3721 aactggacgg gcgacatcgt ctggacgatc gtgcggccga ccgtggtgtg ggggccgtgg
     3781 catccgagct tcggcggctc gacctggaaa tatctcaacc gccgctggta catgatcacc
     3841 ggcggcaagg acccggtgcg gacctattcc tatgtcgata acctcgtcgc gcagctgatc
     3901 gcggcggccg aggcggcgcc ggagcgggtc ggcgccaaga tgttctacgg cggcgacgcg
     3961 ccgatcccga cctcctggtg gctggacggc ttcgctaagg cgctgaccgg caagccggcg
     4021 cggcgggtgc cctatgccct gctgagcgga cttgcctggg ccggcgaggc gatggggcgg
     4081 atcggcggcc cctcgccgat caaccgcggc cggctctacc ggatgaccag cgaatatgtg
     4141 gtgccgctcg acgacacgtt cgaaatactc ggcgaggggc cggtgacgct ggacgagggg
     4201 atcgcccgta cggtcgactg gctgcgcgcc gaccagcccg aggaattcgg gcgcacgaga
     4261 cgggcttagg tcccgcccag gagctcggtc caggcggcga gcgcgcgggc gttgatcgcg
     4321 gcgatggcgg cggctgcata gctacgggtg gcctcgcgcc cgccggcctc ggccgatgcg
     4381 gcggcggggc cgagcgccgc ctcgaaatcg gcggcggctg ccccccgggc gatcgtcagc
     4441 ctcgaataag cgggcagata ttcgagcacg ccgaggtcgg gcgcggcgag cgttgcgcag
     4501 ccggcgctga gcgcttcgag cacggtgagg ccgtaggatt cgaacaggct cgagacgacc
     4561 atcacatcgg tgtcggcgta gaaacgctgg cggtcgccat gcgcgaccgg tccgacgaac
     4621 tcgatgtcgg cgcggcccgc gtggcggcgc tgaacggcgt ggaccgcggc cagatagcgc
     4681 tcgctaccgg cgcgcggggc accgccgacc accagccgca ccggccggga gccggaaggc
     4741 cgggccgcgg cccaggcggc gaagccctcg accagcggca gcaggccctt gcgcgggctg
     4801 atctcggcga ggaagctcaa ggtcagcggg cgatcgccct cccccgctcc ttccgctcgc
     4861 tccgcggcct gagcggccgc cggagcggtc aggtccgggc tgtcgaagaa atcggggatc
     4921 acccattcgg gcgcgcggcg ccgggcggcc gcgatgctat ggtcgcgctc gacctcggag
     4981 gagaagacga tgccgccgct gcggtcgagc agccgccgct cgatgaggtg gaaatagggg
     5041 attttcttga ggtcgcgcag ccggctgaac tcgatccggc cgagcccgcc gcgcggcaga
     5101 tagagcattt gctgcccgtc cggccgccgc ttcagcaacg ggccaagctg cgccgcattc
     5161 tgccacggcc cgacgatcag gatcgcgtcg taatccgggg cggtgtcgag cggaaacggc
     5221 tcggcagcgc gcccaggctc gggcagcagc tcgagcgtct cgatgttgcg gcggaagcgg
     5281 ccgagatcga agacgtcggc ggagatgccg cgcggcgcca gcagattggc catcaaccgc
     5341 accgattccg gcccgccgcc ccagccctgc cggaaattct ggacgacgta caagatcttc
     5401 atgtcaggct ttcccggaag gcgcaggccg ctcgcggcgc cgcccggttc cccccgtcgc
     5461 ggcgccgcgg ccgcagccgg ccggcggcgc ctccgctaga caagcgggcg aaaagacgca
     5521 agcagaccga cgtcagagag tggcgaggaa gctccccagg ccgaggccga gcgcgaacag
     5581 cagcagccaa tcgggcgcca cccgtttgat caaagaatgc acttgcccct ccccgagcaa
     5641 ctcgatgggg gaaagcttcc ttcgcaacag cctgcggagc aacggctttg ttcggccccg
     5701 atgcaatttg gcaacgactg tgactggagc gacacatccg gtagtcagtc cgtctcgcgc
     5761 gcgggttcct ccccggtcag cggctcgccc gtggcccttt ccacggcaag ccccgcggtg
     5821 agcccgtgga ccagagtgga cagcaatatc gtgaacgcca cggtcgccca gagctgatat
     5881 tcgttgacca gctccacatg gtggccggca taagcgagat aatagacgga gccgatgccg
     5941 cgcactccat aaaaggagac gaccagccgc tcgcgccccc gaagcgcggt gccggcgagg
     6001 ctcatccacc cgaccagcgg ccggatcagg aataccagcg ccagcccgat cgccgcatgg
     6061 ctgggctcga aatagggcca gagcagcggg agcgccgccc cgaccgcgac cagcagcatg
     6121 gcggtgagcg aatgctccag cgattc
//
LOCUS       DASVRK010000046         3643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_70072, whole genome shotgun sequence.
ACCESSION   DASVRK010000046 DASVRK010000000
VERSION     DASVRK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70072"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VF589_01745"
     CDS             complement(<1..489)
                     /locus_tag="VF589_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEX8256330.1"
                     /translation="MKLRFNERASARTAAYCLAALAALAAATPAAAGALQVDPIRVDL
                     STDRKTGTVTVSNDAAGPVTIHASALAWSQTGGEDRYQDTNAVIVSPPIFTIPAGGTQ
                     TVRVGLRSAAGAGHAYRLIIEEVPQANPQGGIQVALRLNLPLFAMVQPGSTADLSWAA
                     WQQ"
     gene            complement(515..1021)
                     /locus_tag="VF589_01750"
     CDS             complement(515..1021)
                     /locus_tag="VF589_01750"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEX8256331.1"
                     /translation="MTHIRFAALAAVSLAALTAFPGQAVAQTASSSLGVSATVSPSCT
                     VTTSPVAFGSINAVGGSAVNATGGISVTCTNGTDWTASAGTGAGTSASFASRKMSAGS
                     EVINYSLYTDSGHSSIWGDGSGSTQTIADEGTGAAQSVTIYGRILPGQTTAKAGSYSD
                     TVSVTVSY"
     gene            1309..2010
                     /gene="epsC"
                     /locus_tag="VF589_01755"
     CDS             1309..2010
                     /gene="epsC"
                     /locus_tag="VF589_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX8256332.1"
                     /translation="MLAGLVDYLDSIKARDPAARSRWEVLLYPGVLALGLHRIAHWLF
                     RGELFFLARFVNHFARFLTAIDIHPGAVIGRNFFIDHGFVVIGESAEIGDDVTLYQHV
                     TLGGTNPTNGEPGKRHPTIADGVIIGSGAQVLGPIKVGARARIGANAVVTREVPEGAT
                     MIGIPARPTLVDAAEYSKGFMPYGTPCSEIADPGAHRLEILKCEVEQLRKRVAELIEE
                     RDAGQPLETRERDSA"
     gene            2010..2354
                     /locus_tag="VF589_01760"
     CDS             2010..2354
                     /locus_tag="VF589_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX8256333.1"
                     /translation="MGTVHPFPSSSARPTQSGLERAELSRIIDLYGRMVAAGLWKDYA
                     IEFRGDAAVFWAFRRAAERPEYKIEKRPALRNRQGMWALIGENGAVLRRGHELGPVLA
                     PVERRLMKLVGG"
     gene            complement(2332..3432)
                     /locus_tag="VF589_01765"
     CDS             complement(2332..3432)
                     /locus_tag="VF589_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256334.1"
                     /translation="MYSETDLEAAVAAGALSPDAAQALRNHVAQRRETPAADEEHFRL
                     LTGFNDIFVAIAAVILLVAMSWIGHSLPPNLDYQGPSPFSGVLVAATAWGLAEFFTRK
                     RRMALPSILLLLAFVGGVFFASAVALVLAVGPAEFNDSRATGPLVVAAAAALAAAAAW
                     LHWRRFRVPITIAAGAAAVVGLALALIASAIGTDDTATFERTLLGFGLLLGIGVFLFA
                     MWWDSSDPRRETRRSDVAFWLHLLSAPLIVHPIFTLLGLNDGDATIAEGFAVLLVYFI
                     LGLTALAIDRRALLVSALAYVLWALSDLFERFGAVELNFALTALVIGSALLLLSAFWH
                     NARRAVVRPLPASLQAKLPVIDRVAVSPQPAS"
BASE COUNT          612 a         1178 c         1313 g          540 t
ORIGIN      
        1 ctgctgccag gcggcccagc tgagatcggc ggtcgagccc ggctggacca tcgcgaacaa
       61 cgggaggttg aggcggagcg cgacctggat gccgccttgc gggttggcct gcggcacttc
      121 ctcgatgatc aggcggtagg cgtggccggc gccggcggcg ctgcgcaatc cgacccggac
      181 ggtctgggtg ccgccggccg ggatcgtgaa gatcggcggc gagacgatga ccgcgttggt
      241 gtcctggtag cggtcctcgc cgcccgtctg gctccaggcc aaagcggagg cgtggatggt
      301 gactgggccg gcggcgtcgt tgctgacggt gacggtgccg gtcttgcggt cggtgctgag
      361 atcgacccgg atcggatcga cctgaagcgc gcccgccgcc gccggcgtgg ccgcggcgag
      421 ggcggccaaa gcggccaggc aataagccgc cgtgcgggcc gaagcccgtt cattgaaacg
      481 caatttcatg gccgccctcc cggagcgttg ctgatcagta gctgacggtc accgagacgg
      541 tgtcggaata gctgcccgcc ttggcggtgg tctggcccgg caggatgcgg ccgtagatgg
      601 tcacgctctg cgcggcgccg gtgccctcgt cggcgatggt ctgggtgctg ccgctgccgt
      661 cgccccagat cgagctgtgg ccgctgtcgg tgtagagcga ataattgatc acctcggagc
      721 cggccgacat cttgcggctg gcgaagctgg cgctcgtccc ggcgccggtg ccggcgctgg
      781 cggtccagtc ggtgccgttg gtgcaggtga cgctgatgcc gccggtcgcg ttgaccgcgc
      841 tgccgccgac cgcgttgatc gatccgaacg ccaccgggct ggtggtgacg gtgcagctgg
      901 ggctgacggt ggcgctgacg ccgagcgacg agctcgcggt ctgcgcgacg gcctggcctg
      961 gaaacgccgt cagggcggca agcgaaacag ccgcgagggc tgcgaagcga atgtgggtca
     1021 tatcaaaaat ccctcccatg gaagcgagcc gatcgatccc gatccggacg ctggtcctac
     1081 aatccgtagc tccgcccaac accacgggcg gattaccgct gttctacgag gccgggggtt
     1141 aaaattgtgt taggcttatt tgatcgcctg ttttcaaaaa cttgggcgga tggaatgggg
     1201 cggaaagtgg ccgctgcgat cgcgccccta cgggggatgc cgcaatcgct ggaaatccgg
     1261 gcgcggctgc cttatcttta agggaggcgt tacgaagagc gggtgtcgat gttggcgggc
     1321 ctggtggatt atctggattc gatcaaggca cgcgatcccg ccgcccgttc ccgctgggaa
     1381 gtgctgcttt atcccggggt gctggcgctg ggcctgcacc ggatcgcgca ctggctgttc
     1441 cggggcgaat tgttcttcct cgcccgcttc gtcaaccatt tcgcccgctt cctgaccgcg
     1501 atcgacatcc atcccggcgc cgtgatcggc cgcaacttct tcatcgacca tggcttcgtg
     1561 gtgatcggcg agagcgccga gatcggcgac gacgtgacgc tctaccagca tgtcaccctg
     1621 ggcggcacca acccgactaa cggcgagccg ggcaagcgcc acccgaccat cgccgacggc
     1681 gtcatcatcg gctcgggcgc gcaggtgctg gggccgatca aggtcggcgc gcgcgcgcgg
     1741 atcggcgcca atgccgtcgt gaccagggaa gtgcccgaag gcgcgacgat gatcggcatc
     1801 ccggcccggc cgaccctggt cgacgccgcc gaatattcga aaggcttcat gccctacggg
     1861 acgccgtgca gcgagatcgc ggatcccggc gcgcatcggc tcgaaatatt gaagtgcgag
     1921 gtcgaacaat tgcgcaagcg cgtggccgaa ttgatcgagg agcgggacgc cggccagccg
     1981 ctcgaaaccc gggagcgcga cagcgcctga tgggcacggt ccaccctttt ccgtcatcgt
     2041 ccgcgcggcc gacccagagc ggattagagc gggccgagct cagccgcatc atcgaccttt
     2101 acgggcggat ggtcgcggcc ggcctgtgga aggattatgc gatcgagttt cgcggcgacg
     2161 cggccgtctt ctgggcgttt cgccgcgccg ccgagcggcc cgaatataag atcgaaaagc
     2221 gccccgcctt gcgcaaccgg cagggcatgt gggcgctgat cggggagaat ggcgcggtgc
     2281 tgaggcgggg ccacgagctc ggccccgtcc tcgcacccgt ggagcgccgg cttatgaagc
     2341 tggttggggg ctgaccgcca cccgatcgat caccggaagc ttcgcctgaa gcgacgccgg
     2401 cagcggccgc accaccgccc ggcgcgcatt gtgccagaag gcggagagca gcagcaaggc
     2461 cgatccgatc accaaagcgg tcagcgcgaa gttgagctca accgcgccga accgctcgaa
     2521 cagatcgctg agcgcccaca aaacgtaagc gagcgccgag accagcaagg cccgccggtc
     2581 gatcgccagc gcggtgaggc cgaggatgaa atagaccagc agcaccgcga acccctcggc
     2641 gatggtggcg tcgccgtcgt tgaggccgag cagggtgaag atcggatgga cgatcagcgg
     2701 cgccgacagg aggtgcagcc agaaggcgac gtcggagcgc cgcgtctcgc gccgcgggtc
     2761 ggacgaatcc caccacatgg cgaacaggaa gacgccgatg ccgagcagca ggccgaagcc
     2821 gagcagggtc cgctcgaacg tcgcggtgtc gtcggtgccg atcgccgagg cgatcagcgc
     2881 cagcgcgagt cccaccacgg cggccgcccc ggcggcgatc gtgatcggca cgcggaaccg
     2941 ccgccagtga agccacgccg ccgcggccgc gagcgccgcg gcggcggcga cgacgagcgg
     3001 gcccgtggcg cggctgtcat tgaactcggc cggaccgacg gcgagcacca gcgcgacggc
     3061 cgaagcgaag aacacgccgc cgacgaaggc cagcagcagc aggatgctgg gcagcgccat
     3121 gcggcgcttg cgggtgaaga attcggcgag gccccaggcg gtcgcggcga ccagcacgcc
     3181 cgagaagggc gaggggcctt gataatcgag attggggggc agcgaatgcc cgatccagct
     3241 catcgccacc agcaggatca ccgcggcgat cgccacgaaa atgtcgttga agccggtcag
     3301 cagccggaaa tgctcctcgt ccgcggccgg cgtctcgcgc cgctgggcga cgtgattcct
     3361 cagcgcctgg gccgcgtcgg ggctgagcgc gcccgccgcc acagccgcct cgagatcggt
     3421 ttcgctatac atgtggcatg tcctcctcag gcggggagca tagcaggagt gtattgccca
     3481 tgcaatacac tcctgtcggc cgcgagccgg cgccgcgcgg cgccggccgg cggtcagccg
     3541 accaccgtct cgaagctggc gtggctcaat tggtggccga cgccgatatg cgcgaagatc
     3601 tgctgggtga aggcggcgcc ggagccgtcc gcgtcataga aga
//
LOCUS       DASVRK010000047         9046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_442966, whole genome shotgun sequence.
ACCESSION   DASVRK010000047 DASVRK010000000
VERSION     DASVRK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442966"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="VF589_01770"
     CDS             <1..1688
                     /locus_tag="VF589_01770"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX8256335.1"
                     /translation="VDLMPTVLELMGLEIPAGLSGRSLLPFVSNPGMIDDDAAAVTTM
                     YGSAAIRTNDYRYILYEDGSTELYDIAEDPNQWTNLAGDPAWQQVEEQLDGRLRADLE
                     PQGWLWADAGESVVGSAGNDVLVLAPGTVSARGGGGDDVYFINDLGVKLVEGVGRGID
                     TVHVGDSYTLPDNIENVQLRYRVRGETLIGNALDNDMVGAGRLEGLEGDDRLELRGAG
                     SADGGAGDDLLEGVGGADRLAGGDGNDVIVARGGNDLVGGGAGDDRIGGGDGIDTASY
                     VAAASGVIVRLAIAGAQDTRGAGIDTLTGFENVTGSAFGDRLAGDGGANLLIGGAGSD
                     MLDGGRAIDIMNGGAGNDVFIARDAGDAVIEAGNGGSDEVRSFVNYRLADNVETLRLY
                     GAGRNGTGNDLANSITGTSGHNGLAGLGGNDLLRGGAGTDQLRGGDGNDRLDGGAGTD
                     SMLGGAGADRFVFEGSHVTGLRDEADSIGDFSRAQQDRIDLRAIDADAGAGGDQNFQF
                     LGTGAFSGAAGELRVGTSETGRYVEGDVDGDKVADFFIQVSGTQPLVGTDFIL"
     gene            complement(1696..2358)
                     /locus_tag="VF589_01775"
     CDS             complement(1696..2358)
                     /locus_tag="VF589_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX8256336.1"
                     /translation="MFREIEVLTPPQVAELRQIAAAANFVDGRISNPHSTVKQNLQLH
                     DEASYKRSSQLLTQALLSHEDFRNFAFPVGMLPPLMTRYTPAMRYGAHADAAYLQLGN
                     TSLRSDLSCTIFLSDPKTYEGGALRIQLGTRDMRFRGEAGTAIVYPSNMLHEVERVTQ
                     GERLVAITFIQSRIADNFRREMLYELNEVAALEGLSMAPENFARMQLIQANLLRHWGE
                     RP"
     gene            complement(2367..3392)
                     /locus_tag="VF589_01780"
     CDS             complement(2367..3392)
                     /locus_tag="VF589_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256337.1"
                     /translation="MSEAAASAGLPEAVAASPRWFPFALYPETDQVLLVAKTEAEYRA
                     DSFLDQRSLRPDAERRTVGWAELAGALPAETRRDVQYIFHIGHVGSTLVSRLLGEMGG
                     VFALREPMLLRTFAEMPADWAAPRLDPLTALLSRTFRPDQRALVKATSFTSEIADRLV
                     PPGSRALFLFATPGHYIENILAGDNSRQELHILSPGRLERLRARCPGLTWELDRLSEA
                     RRAALGWACEMTSLERSAAAIARGSVMWMDFDTFLAEPPRHLAAIAGFFGHSLDDEAA
                     AAICSGPLMGRYSKAPEFEYTPALRRDVLAEARFMHGPAIRDALLWLEEAGRRYPAIG
                     SAIRRAQ"
     gene            complement(3469..4089)
                     /gene="rplI"
                     /locus_tag="VF589_01785"
     CDS             complement(3469..4089)
                     /gene="rplI"
                     /locus_tag="VF589_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX8256338.1"
                     /translation="MDVILLERVEKLGAIGDVVKVKNGYARNFLLPNGKALRANEANR
                     KVFEANREKIEATNAERRSAAEKDSKTIDGAKVQLIRQASNTGQLYGSVSARDIVDAL
                     EAAGHKVGKNQVVLDRPIKAIGVQDVRIALHPEVSVTISVNVARSPEEAELQAQGVDV
                     MADMFERDEAGFTEDYDPNAEPGTIDAPIDAGAESTGGDGEAASEA"
     gene            complement(4102..4326)
                     /gene="rpsR"
                     /locus_tag="VF589_01790"
     CDS             complement(4102..4326)
                     /gene="rpsR"
                     /locus_tag="VF589_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX8256339.1"
                     /translation="MARPFFRRRKSCPFSGNDAPKIDYKDVRLLQGFVSERGKIVPSR
                     ITAVSAKKQRELAKAIKRARHLGLLPYVVK"
     gene            complement(4344..4781)
                     /gene="rpsF"
                     /locus_tag="VF589_01795"
     CDS             complement(4344..4781)
                     /gene="rpsF"
                     /locus_tag="VF589_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX8256340.1"
                     /translation="MPLYEHVFLARQDLAQAQVDALAETATNIIESNGGRVVKTETWG
                     LRSLAYRIQKNRKAHYVMLEVEAPAPVVAELERQTQINEDVVRYMTIKVEAHEAGPSA
                     MMRRNERSERGDRGDRDRGPRGDRPDRGDRGDRGPRPDRGFDA"
     gene            4989..5912
                     /gene="fabD"
                     /locus_tag="VF589_01800"
     CDS             4989..5912
                     /gene="fabD"
                     /locus_tag="VF589_01800"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX8256341.1"
                     /translation="MRAFIFPGQGSQAVGMGKTLAEASNLARALFEEVDDALGQGLFR
                     LMAEGPQDALTLTENAQPAIMANALATLRVSGIELADKADFVAGHSLGEYSALCAADA
                     FDLATTARLLKLRGRAMQAAVPVGEGAMAALLGLGLEAAQAVAAEAAQGEVCTVANDN
                     DPNQVVVSGHKAAVDRALEIAKAHGAKRALLLPVSAPFHSPLMKPAARAMEQALGEAD
                     VRPPLVPLFANVTAAQAEDPSELRRLLVEQVTGMVRWRESVAAMAEAGVTHFVEFGGK
                     VLGPMVKRIVPDARTSSVVTMADIEALVKEI"
     gene            6226..6963
                     /gene="fabG"
                     /locus_tag="VF589_01805"
     CDS             6226..6963
                     /gene="fabG"
                     /locus_tag="VF589_01805"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX8256342.1"
                     /translation="MFDLSGMTALVTGASGGIGSAIAAALAGQGARVALSGTREEALK
                     AVQADIAGDAVILPTDLSDSAAVDALVPRAVEAMGKLDILVNNAGVTRDNLAMRMKDE
                     EWSDVIRINLEAAFRLARAALKPMMRARHGRIISITSVVGATGNPGQANYAASKAGLV
                     GMSKALAQEVASRGITVNCVAPGFIASAMTEALPEAQKQALLGRIPAGALGQGSDVAA
                     AVAYLASREAAYVTGQTLHVNGGMAML"
     gene            7060..7296
                     /locus_tag="VF589_01810"
     CDS             7060..7296
                     /locus_tag="VF589_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8256343.1"
                     /translation="MSETADRVKKIVVEHLGVEAEKVTEEASFIDDLGADSLDIVELV
                     MAFEEEFGVEIPDDAAEKITTVKDAIDYIETNKG"
     gene            7445..8701
                     /gene="fabF"
                     /locus_tag="VF589_01815"
     CDS             7445..8701
                     /gene="fabF"
                     /locus_tag="VF589_01815"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX8256344.1"
                     /translation="MRRVVVTGLGLVTPLGGDVETSWRNILASKSGAGRITRFDPSDQ
                     ACTIACEVKPPEHEYGFDPGKRVDHKVQRQVDPFIVYGLDAAGQALEDAGLTEMSEEE
                     RYRVGVSIGSGIGGLPGIEKESLVLHEKGPRRVSPHFVHGRLINLISGQVSIKYGLMG
                     PNHAVVTACSTGAHSIGDAARMIMLDDADVMLAGGAESALCPLGIAGFAQARALSTRN
                     DEPEKASRPYDKDRDGFVMGEGAGVVVLEEYERAKARGARIYAEIIGYGMSGDAYHVT
                     APHPEGAGGYRAMEVAMKRSGLDLSDIDYINAHGTSTPLGDELELGAVRKLFGNAVGK
                     LSMSSTKSAIGHLLGGAGAVEAIFCILAIRDQIVPPTLNLDNPSEGTAGVDLVPHVAK
                     EREVKAVLNNSFGFGGTNASLVMKAV"
     gene            8709..>9046
                     /locus_tag="VF589_01820"
     CDS             8709..>9046
                     /locus_tag="VF589_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX8256345.1"
                     /translation="MRKLAALVLLVVAGLAVGLWFIQGWSGAGPARQPTSVVIAPGTS
                     MVAAANQLEKAGVIGSAERFLLQANLFGGGESIKAGEYEIPARASHSQVLSLLQSAKT
                     LQRMVTVPEGL"
BASE COUNT         1455 a         2901 c         3221 g         1469 t
ORIGIN      
        1 tggtcgacct catgccgacg gtgctggaac tgatgggcct cgagataccg gccggtctgt
       61 cgggacgctc gctgctgccg ttcgtttcga acccgggaat gatcgacgac gatgccgccg
      121 cggtcaccac catgtacggc agcgccgcaa tccgcaccaa cgactatcgc tacatccttt
      181 acgaggatgg ctcgaccgag ctttacgaca tcgccgagga tcccaaccag tggaccaacc
      241 tggcgggcga tccggcctgg cagcaagtcg aggagcagct cgacgggcgc ctgcgggcgg
      301 acctcgagcc gcagggctgg ctctgggccg acgccggaga gagcgtcgtg ggatcggccg
      361 gcaacgacgt gctggtgctg gcgcccggga ccgtctcggc gagaggcggc ggcggcgacg
      421 acgtctactt catcaacgat ctgggggtga agctcgtcga aggcgtcggc cgcggcatcg
      481 atacggtgca tgtcggcgac agctatacct tgcccgacaa tatcgagaat gtgcagctga
      541 gataccgggt gcggggcgag acgctgatcg gcaacgcgct cgacaacgac atggtcggcg
      601 ccggtcgcct cgaaggcctg gaaggcgacg accggctcga gctgcgcggc gcgggttcgg
      661 ccgacggcgg cgccggcgac gacctgctcg aaggcgtcgg cggggcggac cggctggccg
      721 gcggcgacgg caatgacgtg atcgtcgcca gggggggcaa cgacctggtc ggcggcggcg
      781 ccggcgacga ccggatcggc ggcggcgacg ggatcgacac cgcttcctac gtcgccgcgg
      841 cctcgggcgt gatcgtcagg ctcgcgatcg ccggcgcgca ggatacgcgg ggcgcgggaa
      901 tagacacgct gaccggcttc gagaacgtca cggggtccgc attcggcgat cggttggccg
      961 gcgacggcgg cgccaatctc ctcatcggtg gcgccggttc ggacatgttg gacggcggac
     1021 gcgcgatcga catcatgaac ggcggcgccg gcaacgacgt cttcatcgct cgcgacgccg
     1081 gcgacgcggt gatcgaagcc ggcaacggcg ggagcgacga ggtgcgcagc ttcgtcaact
     1141 accgcctcgc cgacaatgtc gagacgctgc ggctctacgg agccggccgc aacggcaccg
     1201 gcaacgatct tgccaacagc atcaccggca cgagcggcca caatgggctt gccgggctcg
     1261 gtggaaacga tcttctacgc ggcggcgccg gcaccgacca gttgcgcggc ggcgacggca
     1321 acgaccgtct cgacggcggc gccggcaccg attcgatgct gggcggcgcc ggggccgacc
     1381 ggttcgtctt cgagggcagc catgtcaccg gacttcgcga cgaggccgat tcgatcggcg
     1441 acttctcgcg ggcgcagcag gaccggatcg accttcgcgc gatcgacgcc gacgcggggg
     1501 cgggcggcga ccagaacttc cagttcctcg gaaccggcgc cttttccggc gccgccgggg
     1561 agcttcgcgt cggaacgtcg gagaccgggc gctatgtcga gggcgacgtc gacggggaca
     1621 aggtcgccga tttcttcatc caggtctccg gaacgcagcc gctcgtcggg acggatttca
     1681 tcctctagcg ccgcctcagg gacgctcgcc ccagtgacgg aggaggttgg cctggatgag
     1741 ctgcatgcgc gcgaaattct ccggcgccat gctgagccct tcgagcgccg ccacctcgtt
     1801 gagctcgtag agcatctcgc ggcgaaaatt gtcggcgatt cggctctgga tgaaggtgat
     1861 cgccaccagc cgctcgccct gcgtcacccg ctccacctcg tggagcatgt tggacgggta
     1921 gacgatcgcg gtccccgcct cgccgcggaa ccgcatgtcg cgcgtgccga gctggatgcg
     1981 aagcgcgccg ccttcatagg ttttcgggtc gctcaggaag atggtgcagc tgaggtcgga
     2041 gcgcagcgag gtgttgccga gctggagata ggcggcgtcg gcatgggcgc cgtagcgcat
     2101 cgccggcgtg tagcgcgtca tcagcggcgg cagcatgccg accgggaagg cgaagttgcg
     2161 gaaatcctcg tgcgagagca gcgcctgggt gagcagctgc gaggaccgtt tgtaggaggc
     2221 ctcgtcgtgc agctggaggt tctgcttgac cgtggaatgg gggttgctga tgcggccgtc
     2281 gacgaaattg gcggcggcgg cgatctgccg cagctcggcc acctggggcg gcgtcaaaac
     2341 ttcgatttcc ctgaacattg ccgctcctat tgcgcgcgcc gaatcgccga accgatggca
     2401 ggatagcgtc tgccggcctc ttccagccag agcagcgcgt cgcggatcgc cggtccgtgc
     2461 atgaagcggg cctcggccag cacgtcccgc cgaagcgcgg gcgtatattc aaattcgggc
     2521 gccttcgaat agcgccccat cagcgggccc gagcaaatag cagccgccgc ttcgtcatcc
     2581 agggagtggc cgaagaagcc ggcgatggcg gcgaggtggc gcgggggttc ggcgaggaac
     2641 gtatcgaagt ccatccacat gacgctgccc cgggctatgg cggcggcgct gcgttcgagc
     2701 gaggtcatct cgcacgccca gccgagcgcg gcgcggcggg cttcggagag ccgatctagc
     2761 tcccacgtca ggcccgggca gcgggcgcgc aggcgctcga gccggcccgg gctgaggatg
     2821 tgcagctcct ggcgcgaatt atcgccggcg aggatgttct cgatgtaatg gccgggcgtc
     2881 gcgaacagga acaaggcgcg cgagccggga ggcacgagcc ggtcggcaat ctcgctggtg
     2941 aagctggtcg ccttgacgag ggcgcgctga tccgggcgga aagtccgcga cagcaaagcg
     3001 gtcagcggat cgagccgcgg cgccgcccag tccgccggca tctcggcgaa ggtcctgagc
     3061 agcatcggct cgcgcagcgc gaagacgccg cccatctcac cgagcaggcg cgagacgagg
     3121 gtcgagccga catggccgat gtggaagata tactggacgt cgcggcgggt ctcggcgggg
     3181 agggcgccgg ccagttccgc ccagccgacc gtgcggcgct cggcgtcggg gcgtagcgag
     3241 cgctggtcga ggaagctgtc ggcgcgatat tccgcctcgg tcttggcgac gagcagaacc
     3301 tggtccgtct caggatagag cgcgaagggg aaccagcgtg ggctcgccgc caccgcttcg
     3361 gggaggcccg cacttgccgc cgcctcgctc atcctacttg ctcctcaaac ggaaaagccg
     3421 cccgctccag ggagcaagcg gcttttcatc acgcagcctt ggctcggctc aggcctcgct
     3481 ggcggcctcg ccgtcgccgc cggtgctctc ggcgccggcg tcgatggggg cgtcgatcgt
     3541 gccgggttcg gcattgggat cgtaatcctc ggtgaagccg gcttcgtcgc gttcgaacat
     3601 gtcggccatg acgtcgacgc cctgtgcctg gagctcggcc tcttcgggcg agcgggcgac
     3661 gttgacgctg atcgtcaccg acacttccgg atggagggcg atcctcacgt cctgaacgcc
     3721 gatcgccttg atcgggcggt cgaggacgac ctggttcttg cccaccttgt gaccggcggc
     3781 ttccagcgcg tcgacgatgt cgcgtgcgga caccgaaccg tagagctggc cggtattcga
     3841 agcctggcgg atcagctgca ccttggcgcc gtcgatggtc ttcgaatcct tctcggccgc
     3901 gctgcggcgc tcggcgttgg tggcctcgat cttctcgcga ttggcctcga acaccttgcg
     3961 gttggcttcg ttggcacgca gcgccttgcc gttgggaagg aggaagttgc gggcatagcc
     4021 gttcttcacc ttcaccacgt cgccgatggc gccgagcttc tcgacgcgtt caagcagaat
     4081 gacatccatc ttgtcggctc cttatttcac gacgtagggg aggaggccga gatggcgcgc
     4141 gcgcttgatc gccttggcga gttcgcgctg cttcttggcc gagacggcgg tgatgcggct
     4201 gggcacgatc ttgccgcgct cggagacgaa gccctggagc aggcggacgt ccttatagtc
     4261 gatcttgggc gcatcgttgc cggagaaagg gcagctcttg cggcggcgga aaaatgggcg
     4321 ggccatgttg ctgatccttt cgcttaagcg tcgaagccac ggtcgggacg ggggccccgg
     4381 tcgccgcggt cgccgcggtc gggacggtcg ccgcgcgggc cgcgatcgcg atcgccgcgg
     4441 tcgccgcgtt cgctgcgctc gttgcggcgc atcatcgccg acgggccggc ttcgtgagcc
     4501 tcgaccttga tggtcatgta gcggaccacg tcctcgttga tctgggtctg gcgctcgagc
     4561 tcggcgacga cgggcgcggg ggcctcgact tcgagcatca cataatgcgc cttgcggttc
     4621 ttctggatcc ggtaggcgag cgagcgcagg ccccaggtct cggtcttgac gacccggccg
     4681 ccgttcgatt cgatgatgtt ggtggcggtt tccgccagcg catcgacctg ggcctgagcc
     4741 agatcctgac gcgcgagaaa gacatgctcg taaagcggca tgttccacgt ccttttcttt
     4801 tggccgatcg ctgacgccgc accatgcgaa cgcccctccg gctgtcgtct ctcaatgcaa
     4861 accggggctg gaagcatcgc ctccgccccg gatgaggggt gcccatggcg gaaaggctcg
     4921 gaaaaggcaa gcctcgcttg cgcttgccgc gccgcctgtc ctaccagccg cgccgacagg
     4981 agggctagat gcgcgcattc attttcccgg gccaggggag ccaggcggtc ggcatgggca
     5041 agacgctggc ggaggcgagc aatctcgccc gcgctttgtt cgaggaagtc gacgatgcgc
     5101 tgggccaggg gttgttccgg ctgatggcgg aggggcccca ggacgcgctc acgctgaccg
     5161 agaatgccca gccagcgatc atggccaatg cgctggcgac gctgcgcgtc tccgggatcg
     5221 agcttgccga caaggccgat ttcgtcgccg gccacagcct cggcgaatat tcggctttgt
     5281 gcgccgcgga cgcgttcgac ctcgccacca cggcgcggct cttgaagctg cgcggccgcg
     5341 ccatgcaggc ggcggtgccg gtcggcgagg gggcgatggc ggctttgctg gggctcggcc
     5401 tcgaagccgc gcaggcggtg gcggcggagg cggcgcaggg cgaggtctgc acggtcgcca
     5461 acgataacga cccgaaccag gtggtggtgt cggggcacaa ggcagcggtg gatcgggcgc
     5521 tggagatcgc caaggcgcat ggggccaagc gcgcgctgct gctgccggta tcggcgcctt
     5581 tccactcgcc gctgatgaag cctgccgcgc gggcgatgga gcaggcgctg ggcgaggccg
     5641 atgtccggcc gccgttggtg ccgctgttcg ccaacgtgac ggcggcccag gcggaggacc
     5701 cgtccgagct tcgccggctg ctggtggagc aggtgaccgg gatggtgcgc tggcgcgaga
     5761 gcgtcgccgc catggccgag gcgggcgtga cccacttcgt cgaattcggc ggcaaggtgc
     5821 tcggccccat ggtcaagcgc atcgtgcccg acgcgcggac gagcagcgtc gtcacgatgg
     5881 ccgacatcga ggcgctggtg aaggagatct agattcgcct caaagggtcg atctccgcca
     5941 cctctgcccc gagcgtaccc tcagcggcct aactgactgt ctgtgtgcga gtaaccgtcg
     6001 tcataggcct cattctcttc ttggtgctga tccatcttgt cggaactcca cgtcagcacc
     6061 gagtcaagta gcccgtgagg cggctcgtac cgatcatttg agcgatcctc ttggcctttg
     6121 tcgtgataat actccctacc catgttcctc tcctctgcag tccccgcata aagcataacg
     6181 tttcaagggc atttgttttc cattcattcc accaggattc ttgctatgtt cgatctttca
     6241 ggaatgaccg cgctggtgac cggcgcatcg ggtggcatcg gatcggcgat cgcggcggcg
     6301 ctggcggggc agggggctcg ggtggcgctt tcggggacgc gcgaggaggc gctgaaggcg
     6361 gtgcaggccg acatagcggg tgatgcggtg atcctgccga ccgacctgtc ggattcggcg
     6421 gcggtcgacg cgctggtgcc gcgggcggtg gaggcgatgg gcaagctcga catcctcgtc
     6481 aacaatgccg gcgtgacccg cgacaatctc gccatgcgga tgaaggacga ggaatggtcc
     6541 gacgtcatcc gcatcaacct cgaagccgcc ttccggctgg cccgggccgc gctgaagccg
     6601 atgatgcggg cgcggcacgg gcggatcatc tcgatcacgt cggtggtcgg cgccacgggc
     6661 aatccggggc aggccaatta cgccgcgtcc aaggcgggac tggtcggcat gtccaaggcg
     6721 ctggcgcagg aagtcgccag ccgcggcatc accgtcaatt gcgtcgcgcc cggcttcatc
     6781 gcgtcggcga tgaccgaggc cctgccggag gcgcagaagc aggccctgct cggccgcatc
     6841 ccggcgggag cgctggggca ggggagcgac gtcgccgcgg ccgtggccta tctggcgagc
     6901 cgggaagccg cctatgtcac cggccagacg ttgcacgtca acggcggcat ggccatgttg
     6961 tagcttgcgg cgggccgcag ccgcgggccg gccacttgcc agcgccgggc gggaacggct
     7021 agggcgactg caaacaacgc ctctagggaa gggtacacta tgagcgagac cgccgaccgg
     7081 gttaagaaga tcgtcgtcga gcatcttggc gtcgaagccg agaaggttac cgaggaagcc
     7141 agcttcatcg acgacctcgg cgccgacagc ctcgacatcg tcgagctggt gatggcgttc
     7201 gaggaggaat tcggggtcga gatcccggac gatgccgccg agaagatcac gaccgtcaag
     7261 gacgcgatcg actatatcga gacgaacaaa ggctgacccc tcacgaggcg aatagcaccg
     7321 gcccgacgat gatcgagccg cttgtcggaa acggctcccc gtcagggatc acccaccgaa
     7381 gcgacgtttc ggcgggatcc catggccggg gagccgtttt gccagttcac acgggagttt
     7441 tcgcatgcgc cgcgtagtcg ttaccggtct cgggctcgtc actccgctcg gcggcgatgt
     7501 cgagacgagc tggaggaaca ttctggcctc gaagagcggc gccgggcgga tcacccggtt
     7561 cgatccgtcc gatcaggcct gcaccatcgc ctgcgaggtg aagccgcccg agcatgaata
     7621 tggcttcgac cctggcaagc gcgtcgacca caaggtccag cgccaggtcg acccgttcat
     7681 cgtctacggc ctcgacgccg ccggccaggc gctcgaggat gccggcctca ccgagatgag
     7741 cgaggaagag cgctaccggg tcggcgtgtc gatcggttcc ggcatcggcg gccttcccgg
     7801 catcgagaag gaatcgctgg tcctgcacga gaaggggccg cgccgcgtca gcccgcattt
     7861 cgtccacggc cggctgatca acctgatctc tggccaggtc tccatcaaat acggcctgat
     7921 gggccccaat cacgcggtcg tcaccgcctg ctcgaccggc gcccattcga tcggcgacgc
     7981 cgcgcggatg atcatgctcg atgacgccga cgtgatgctc gccggcgggg cggaaagcgc
     8041 gctctgcccg ctcggcatcg ccggtttcgc gcaggcacgg gcgctatcca cccgcaacga
     8101 cgagccggaa aaggcctcgc gtccctacga caaggatcgc gacggcttcg tcatggggga
     8161 gggcgccgga gtggtggtgc tcgaggaata tgagcgggcc aaggcgcgcg gggcgaggat
     8221 ctacgcggaa atcatcggct acggcatgtc gggcgatgcc tatcatgtca ccgcgccgca
     8281 tccggagggc gccggcgggt accgcgcgat ggaagtggcg atgaagcgct ccggcctcga
     8341 cctcagcgac atcgactata tcaacgccca cggtacctcg accccgctcg gcgacgagct
     8401 cgagctgggc gcggtccgca agctgttcgg caacgcggtc ggcaagctgt cgatgagctc
     8461 gaccaagtcg gccatcggcc atttgctcgg cggcgcggga gcggtggagg cgatcttctg
     8521 cattctcgcg atacgcgacc agatcgtgcc gccgacgctg aacctcgaca atcccagcga
     8581 gggcacggcg ggcgtcgatc tcgtcccgca cgtcgccaag gagcgcgagg tgaaggcggt
     8641 gctgaacaac agcttcggct tcggcggcac caacgccagc ctggtgatga aggcggtctg
     8701 acgcaggcat gaggaagctc gccgcgctcg tcctgctggt cgtggcgggc ctcgccgtcg
     8761 gcctgtggtt catccagggc tggagcgggg ccgggcccgc ccgccagccg acgtcggtgg
     8821 tgatcgcccc cggcaccagc atggtggcgg cggccaacca gctggagaag gccggggtga
     8881 tcggctcggc cgaacgtttc ctgctccagg ccaacttgtt cggcggcggc gaatcgatca
     8941 aggccggcga atatgagatc cccgcccggg ccagccactc gcaggtgctt tccctcctgc
     9001 agagcgccaa gaccctccag cgaatggtga cggtccccga aggtct
//
LOCUS       DASVRK010000048         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_443396, whole genome shotgun sequence.
ACCESSION   DASVRK010000048 DASVRK010000000
VERSION     DASVRK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443396"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..712)
                     /locus_tag="VF589_01825"
     CDS             complement(23..712)
                     /locus_tag="VF589_01825"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8256346.1"
                     /translation="MDDIIDCLSPNFDERTTPVSILVLHYTGMTDGAAAVERLCDPAS
                     KVSCHYLVAEDGKVLRMVPEDKRAWHAGQSYWRGMMNLNSDSIGIEIVNPGHEFGYRP
                     FTEQQMDALIPLVAGIVKRHNIPRANVVGHSDIAPTRRQDPGELFEWNRLAKVGLALP
                     RPTRNLMDPQWTHAGFLLALERFGYDVSDPMAAIVAFQRRFRPELIDGEVDAECRMIL
                     LALLLPRPQGD"
     gene            complement(759..1619)
                     /locus_tag="VF589_01830"
     CDS             complement(759..1619)
                     /locus_tag="VF589_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX8256347.1"
                     /translation="MEVTDAASRILAHLLETRTGQQLTLGRRWRIETALAPLMRERGI
                     AGIEQLVSTLVAALDPRLADEVVEALLNNETSFYRDRRSFELLLGGILPRLHAARAAQ
                     KRLRIWCAGCATGQEAYSLALEFAEDIAHWAGWRIELLGTDVSRAAVEQARSGLYSQF
                     EAQRGLPVRQMIRWFGEEPGQRWRIAPQLREAVRFQVHNLLDPPPAPGAFDIILCRNV
                     LLYFPEATRRAAFGNLARAAAPDACLMLGAGETIIGQTDDFIPDPDHRGLYLRAPAAA
                     ANQTARRARA"
     gene            complement(1610..>1882)
                     /locus_tag="VF589_01835"
     CDS             complement(1610..>1882)
                     /locus_tag="VF589_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX8256348.1"
                     /translation="PSVDTMLWAVSETFGPGAVGVVFSGMGRDGLVGSARIVDRGGSI
                     MAQDQRSSAVWGMPRAVAEAGLACAMLAPADLARRLAASAAGAAWR"
BASE COUNT          302 a          612 c          687 g          281 t
ORIGIN      
        1 ccgactttct ggcctgccgg gcctagtcgc cttgtgggcg cggaagcaag agcgccaaca
       61 ggatcatccg gcactccgca tcgacctcgc cgtcgatcag ctcggggcga aagcggcgct
      121 ggaaggcgac gatcgcggcc atgggatcgc tgacgtcata gccgaaccgt tccagcgcca
      181 gcaggaagcc cgcgtgagtc cactgcgggt ccatcaaatt gcgggtcggc cgcggcagcg
      241 cgaggccgac cttggcgagc cggttccact cgaacaattc gcccggatcc tgccggcggg
      301 tcggggcgat gtcggaatgg ccgaccacat tggcgcgcgg gatattgtgg cgtttgacga
      361 tgccggcgac cagcgggatc agcgcatcca tctgctgctc ggtgaacggg cggtagccga
      421 attcgtggcc cgggttgacg atctcgatgc cgatgctgtc ggaattgagg ttcatcatcc
      481 cgcgccaata ggattggccg gcatgccagg cgcgcttgtc ctcgggcacc atgcgcagga
      541 ccttgccgtc ctcggcgacg agatagtggc aggacacctt cgaggcggga tcgcacaggc
      601 gctcgaccgc cgcggcgccg tccgtcatcc cggtatagtg gagcaccagg atgctgaccg
      661 gcgtcgtccg ctcgtcgaaa ttgggcgata ggcagtcgat gatgtcgtcc atgccttact
      721 tgcgctccaa aaagccggtt gcgtcaaggg gggcgggatc aggcgcgggc gcggcgcgcc
      781 gtctggttgg cggcggccgc gggcgcgcga aggtagaggc cgcgatgatc tggatcgggg
      841 atgaagtcgt cggtctggcc gatgatcgtc tcgccggcgc cgagcatcag gcaggcatcg
      901 ggcgccgcgg cccgggctag gttgccgaac gcggcgcggc gggtggcctc gggaaaatag
      961 agcaggacgt tgcggcacag gattatgtcg aaagcgccgg gcgcgggcgg cggatcgagc
     1021 aaattgtgga cttggaaccg caccgcctcg cgcagctgcg gggcgatccg ccagcgctgg
     1081 cccggctcct cgccgaacca gcggatcatc tggcggaccg ggagcccgcg ctgcgcctcg
     1141 aactgggaat agaggccgga gcgcgcctgc tcgaccgcgg cccgcgagac gtcggtgccg
     1201 agcagctcga tccgccagcc ggcccagtgg gcgatatcct cggcgaactc cagagcgagc
     1261 gaataagctt cctggccggt ggcgcagccg gcgcaccaga tcctgagccg tttctgcgcg
     1321 gcgcgggcgg cgtgcaggcg tggcaggatg cctccgagca atagttcgaa ggatcggcgg
     1381 tcgcggtaga aggaggtctc gttgttgagc agcgcctcga ccacctcgtc ggcaagccgt
     1441 ggatcgagcg cggcgaccaa agtcgagacc agttgctcaa tgccggcaat gccgcgctcg
     1501 cgcatcagcg gcgccagcgc ggtctcgatc cgccagcgcc ggccgagggt cagctgctgt
     1561 ccggtccggg tttcgaggag atgggccagg atgcggcttg ccgcgtccgt tacctccatg
     1621 cggcacccgc ggcgctggcg gcgagccgcc gggcaaggtc ggcgggcgcg agcatggcgc
     1681 aggcaagccc cgcctccgcg actgcacggg gcattcccca caccgccgag cttcgctgat
     1741 cctgggccat gatcgagccg ccgcgatcga cgatgcgggc gctgccgacg aggccgtcgc
     1801 ggcccatgcc gctgaacacg acgccgacgg cgccgggacc gaaggtctcg ctcaccgccc
     1861 acagcatcgt gtcgaccgat gg
//
LOCUS       DASVRK010000049         4777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_70699, whole genome shotgun sequence.
ACCESSION   DASVRK010000049 DASVRK010000000
VERSION     DASVRK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70699"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VF589_01840"
     CDS             complement(<1..868)
                     /locus_tag="VF589_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8256349.1"
                     /translation="MQAAIYGFSGTGLTADERDFFRDADPAGYILFQRNCESPEQLRA
                     LTAELRALHGRGDVPILIDQEGGRVARMRPPAWPEFPEAGRFAELYAKAPISAIEAAR
                     VNAEAIAVMLSEVGINVNCLPLLDVRQAGASDIIGDRALGTEPMQVAALGRAVIDGLR
                     SGGVVGVVKHMPGHGRARSDSHVELPVVDAPEEALELDLEPFRTLRDAPMGMTCHIVY
                     TAWDAGRAASLSPVVIGDRASGDDVLQVASLGRAVLDGLAAGGVVGVVKHMPGHGRAR
                     ADSHHQLPLIDAD"
     gene            complement(939..2864)
                     /locus_tag="VF589_01845"
     CDS             complement(939..2864)
                     /locus_tag="VF589_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256350.1"
                     /translation="MASLLETEAPAAAGGVTPRQTLPEALPELTPARFAALRVVWLLA
                     LAIAIVVPLAGTWFLYQERQRIDAPFLELDLPYYGTSEGEIRLLAPWGAAAKASGVAQ
                     DDLILSVEGRPVPRDYDERIAIADLIRTAPGPLVTIETQSSDGRVRTHRLPRSSAQAE
                     EAYARNGISRTAEQMAGTITYHFAVGFMIVAGVLLFRRRGRDPVAALLSLSFLLLTVY
                     GSQGYAFFAAMGAPYLNVILIAIGWTCQFLALMTFPQGRFEPRWTLALAAILIAWAIV
                     LPLGLIPDIAENLGGLAIGCACLAAMMVRYRRLAPGVQRQQIRWTLFGVTAGAVLAVL
                     WVGAGAVIPLIDDSRLRVWLSLVAIVLTPLSFSLPPLGLLVSLLRYRLYDADAVISRS
                     AGYAVLTLLLGAVFAGSAEALELFFEANLGREAGSMPGAIGAGLTVLLINPLHSRIEA
                     WAERRFQRALLHLRNDLPQHLLDIRDTATSKQLLAMVLARIASGVRASRAAVLLVEGG
                     APYLAAERDVTAEAAAEWSAGWIPPLIEQGLDCDCADPLFPLRLALTVDGGGSAATIG
                     WILLGPRPDGSFYGKEEREALEEVAAPIARALQIVRRHERREAEAQSRAEAQEGRIAD
                     MERKLLAALALGGAGRS"
     gene            complement(3014..3748)
                     /locus_tag="VF589_01850"
     CDS             complement(3014..3748)
                     /locus_tag="VF589_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8256351.1"
                     /translation="MTDIRADEPTGPDTAPEDAERLPWLEAVEDDDDGDGPSAAKLIA
                     FVVIGLIAIGLIVGGLFWMGNRGGGGTAGGEGEMIAAPEGDYKVKPGDPGGMKVEGEG
                     DTAFAASEGADPKGAINPGAMPEEPLLKAPPPTNAPTNAPAAAEPKQPAPSPTPSPAA
                     PAAPAAPAGAGGAVQLGAFSSQAGANAAWKALSGRFGYLAPLTHSVTPATVGGKTLYR
                     LRASGPDAKGLCGRLRVAGESCVPID"
     gene            complement(3748..>4777)
                     /locus_tag="VF589_01855"
     CDS             complement(3748..>4777)
                     /locus_tag="VF589_01855"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX8256352.1"
                     /translation="RTVELMILLIKEDLAKLGVHHDLFSSEQEVQESGAVDRAFALLG
                     EKGLVYRGTLPPPKGKTVEDWEPVELLLFRSTEYGDDQDRPMKKSNGSWTYFGADAAY
                     HLQKAEKADALINIWGADHSGTVKRIQAAVSALTGGRVELDVKIVNMVRLFRAGEPIK
                     MSKRAGNFVTLADVVREVGRDVVRFMMLTRRADAPLDFDFAKVVEASKDNPVFYVQYA
                     HARISSLMRKAKAEGIERSPEAAVDLALLDDDELALVRLGAQFPRIVEAAAEAHEPHR
                     IAFYLGDLAAAFHAQWNKGNDDPSKRFLLGHSPAMTRARLELAAAIGQIIRNGLSIMG
                     VAAAEEMQ"
BASE COUNT          727 a         1691 c         1670 g          689 t
ORIGIN      
        1 cgtccgcgtc gatcagcggc agctggtggt ggctgtcggc gcgggcgcgg ccgtggccgg
       61 gcatgtgctt gaccacgccc accaccccgc ccgccgcgag gccgtcgagc accgcccggc
      121 ccagcgaggc gacctgcagc acatcgtcgc ccgaggcccg gtcgccgatc accaccggcg
      181 acaggctggc cgctcgcccg gcgtcccagg cggtgtagac gatgtggcag gtcatcccca
      241 tcggcgcgtc gcgcaaggtc cggaacggct cgaggtcgag ctccaaagct tcctccggcg
      301 catcgaccac cggcagctcg acgtggctgt cgctgcgcgc gcggccgtgg ccgggcatgt
      361 gcttgaccac gccgacgacg ccgccgctgc gcagcccgtc gatcacggcc cgcccgagcg
      421 ccgccacctg catcggctcc gtgccgagcg cgcggtcgcc aataatgtcg ctcgcccccg
      481 cctgccgcac gtcgagcagg ggcaggcaat tgacgttgat gccgacctcg ctgagcatga
      541 ccgcgatcgc ctcggcattg acgcgcgccg cctcgatggc gctgatcggc gccttggcgt
      601 aaagctcggc gaaacggccg gcctcgggga attcgggcca ggcgggcggg cgcatccgcg
      661 cgacgcggcc gccttcctgg tcgatcagga tcggcacgtc gccgcggccg tggagcgcgc
      721 gaagctcggc ggtgagcgcg cgcagctgct ccgggctttc gcaattgcgc tggaaaagga
      781 tgtagccggc cgggtccgcg tcgcggaaga agtcgcgctc gtcggccgtc aggccggtac
      841 ccgaaaagcc gtaaatcgcc gcctgcatgt cagtccctaa tcgcccattc gccagttggg
      901 cgacctagcc gaaagcggac aagcggcgcc acccggaatc acgacctgcc ggcgccgccg
      961 agcgccagcg cggcgagcag cttgcgctcc atgtcggcga tccgcccttc ctgggcttcg
     1021 gcgcgcgact gagcctcggc ctcgcgccgc tcgtgccgcc gcacgatctg cagcgcgcgg
     1081 gcgatcgggg ccgccacctc ctccagcgct tcgcgctctt ccttgccgta gaagctgccg
     1141 tccggccgcg gcccgagcag gatccagccg atcgtcgccg cgctgccgcc gccgtcgacg
     1201 gtcagcgcca gccgcaacgg gaagagcggg tcggcgcaat cgcaatcgag gccctgctcg
     1261 atcagcggcg ggatccagcc ggcgctccat tcggccgccg cctcggcggt gacgtcgcgt
     1321 tcggcggcaa ggtaaggcgc cccgccctcg accagcagca ccgccgcccg cgaggccctg
     1381 acgccgctcg cgatccgcgc cagcaccatg gccagcaatt gcttcgaggt tgcggtgtcg
     1441 cggatgtcga gcagatgctg cggcaggtca ttgcgcaggt ggagcagcgc gcgctggaag
     1501 cggcgctccg cccaagcctc gatccgcgag tggagcgggt tgatcaggag gacggtcagc
     1561 ccggcgccga tcgcccccgg catcgaaccc gcctcgcggc cgagattggc ctcgaagaac
     1621 aattccagcg cctcggcgct gcccgcgaac accgcgccca gcagcagggt gagcaccgca
     1681 tagccggccg accggctgat caccgcatcg gcgtcgtaga gccggtagcg cagcagcgag
     1741 acgagcaggc cgagcggcgg cagcgagaag gagagcggcg tcagcacgat cgcgaccagc
     1801 gacagccaca cccttaggcg gctgtcgtcg atgagcggga tcaccgcgcc ggcgccgacc
     1861 cagaggacgg cgaggacagc cccggcggtg acgccgaaca gggtccagcg tatctgctgg
     1921 cgctgcaccc ccggcgccag ccgccggtag cgcaccatca tcgccgcgag gcaggcgcag
     1981 ccgatcgcga gcccgcccag attctcggct atgtcgggga tgaggccgag cggcagcacg
     2041 atcgcccagg cgatcaggat cgccgccagc gccaatgtcc agcgcggctc gaaccggccc
     2101 tgcgggaagg tcatcagcgc caggaattgg caggtccagc cgatcgcgat caggatgacg
     2161 ttgagatagg gcgctcccat cgccgcgaaa aacgcatagc cttgcgatcc gtagacggtg
     2221 agcaggagga aggagagcga cagtaaagcc gccaccggat cgcgcccgcg ccggcggaac
     2281 agcagcacgc cggcgacgat catgaagccg acggcgaaat gataggtgat ggtgccggcc
     2341 atctgctcgg cggtccggct gatgccgttg cgggcatagg cttcctcggc ctgcgcgctg
     2401 ctgcgcggca ggcggtgggt gcgtacgcgg ccgtcgctgg actgggtctc gatcgtgacc
     2461 agcggccccg gcgcggtccg gatcaggtcg gcgatcgcga tccgctcgtc ataatcgcgc
     2521 ggcaccggcc ggccttcgac gctgaggatc aggtcgtcct gcgcgacgcc cgacgccttc
     2581 gccgccgctc cccatggcgc cagcagccgg atctcgccct cggaggtgcc gtaataagga
     2641 aggtcgagct cgaggaaggg cgcgtcgatg cgctgccgct cctgatagag gaaccaggtg
     2701 ccggccagcg gcaccacgat ggcgatggcg agcgcgagca gccagaccac ccgaagcgcc
     2761 gcgaaccgtg ccggggtcag ctccggaagc gcttccggca gcgtctggcg gggcgtcacc
     2821 ccgccggcag cggccggtgc ttccgtctcc agcaggctag ccacgtcatt ccccccgcag
     2881 ccttatccca taaggcgaga gcgtaacgcc gcggctggct tatacaagcc ccgcgctggg
     2941 tgctccccgg cgaacgccgg ggtccagaag tcggcgggac tgaaccccgg ctttcgccgg
     3001 ggtacgttcg gcctcaatcg atcgggacgc agctttcgcc ggcgacgcgg aggcggccgc
     3061 acaggccctt ggcgtccggg ccgctcgccc gcagccggta aagcgtcttg ccgccgaccg
     3121 tggccggggt gacgctgtgg gtgagcgggg cgagatagcc gaaccggccg gacagcgcct
     3181 tccaggcggc gttggcgccg gcctggctgg agaaggcgcc cagctggacg gcgccgccgg
     3241 cgccggccgg agcggccggg gccgccggcg cagcgggcga aggtgtcggc gaaggtgcgg
     3301 gctgcttggg ctcggccgcg gccggcgcat tggtcggcgc gttggtcggg gggggcgcct
     3361 tgagcagcgg ctcctccggc atcgcgccgg ggttgatggc gcctttcggg tcggcgccct
     3421 cgctggcggc gaaggcggtg tcgccctcgc cttcgacctt catgccgccc gggtcgccgg
     3481 gcttgacctt gtaatcgcct tcgggcgccg cgatcatctc gccctcgccg ccggcggtgc
     3541 cgccgccgcc gcgattgccc atccagaaca gtccgccgac gatcaggccg atcgcgatca
     3601 ggccaatcac cacgaacgcg atcagcttgg cggccgaggg gccgtcgcca tcgtcatcgt
     3661 cctcgaccgc ttcgagccac ggcaggcgct cggcatcctc gggcgcggtg tcgggcccgg
     3721 tcggctcgtc ggcgcgaatg tcggtcatca ctgcatctcc tcggcggcgg ccacacccat
     3781 gatgctgagg ccattacgga taatctgccc gattgccgcc gcgagttcca gtctcgcccg
     3841 tgtcattgcg ggactatgtc ccaataaaaa acgcttcgag ggatcgtcat tacctttgtt
     3901 ccactgggcg tggaacgcgg ccgccagatc gccgaggtaa aaggcgatcc ggtgcggctc
     3961 gtgcgcttcc gccgcggctt cgacgattcg ggggaattgc gcgcccagcc tgaccaaggc
     4021 gagctcatcg tcgtcgagca aagcgaggtc gaccgccgcc tccggcgacc gctcgatccc
     4081 ctccgccttg gccttgcgca tcagcgagga gatgcgggca tgggcatatt ggacgtagaa
     4141 gacgggattg tccttcgacg cctcgaccac cttggcgaaa tcgaaatcga gcggcgcgtc
     4201 ggcgcggcgg gtcagcatca tgaaccggac cacgtccctg ccgacctcgc ggaccacgtc
     4261 ggccagggtg acgaaattgc cggcccgctt ggacatcttg atgggctcgc cggcacggaa
     4321 caggcggacc atgttgacga tcttgacgtc gagctccacg cgcccgccgg tcagcgccga
     4381 gaccgcggcc tggatgcgct tcaccgtccc cgaatggtcg gcgccccaga tgttgatcag
     4441 cgcgtcggcc ttttctgcct tctggaggtg ataggcggcg tctgcgccga aataggtcca
     4501 gctgccgttc gacttcttca tcggccggtc ctggtcgtcg ccatattcgg tcgagcggaa
     4561 gaggagcagc tcgaccggtt cccaatcctc caccgtcttg cccttgggcg gcggcagagt
     4621 gccccggtag acgaggccct tctcgccgag caaagcgaag gcgcggtcga cggcgccgga
     4681 ttcctggacc tcctgctccg aggagaagag atcgtgatgg actccgagct tcgccaagtc
     4741 ctccttgatc aggaggatca tgagttcgac ggtccgc
//
LOCUS       DASVRK010000050         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_381324, whole genome shotgun sequence.
ACCESSION   DASVRK010000050 DASVRK010000000
VERSION     DASVRK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381324"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VF589_01860"
     CDS             <1..285
                     /locus_tag="VF589_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8256353.1"
                     /translation="HDYKVVFVGDAAMSPYEITHAGGSVEHYNDEAGAVWVQRMATTY
                     PSAAWLNPTPEQYWDYSHSTQLVRQLMQGRMYPLTLDGLDDAMRALSRKS"
     gene            complement(313..1857)
                     /locus_tag="VF589_01865"
     CDS             complement(313..1857)
                     /locus_tag="VF589_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256354.1"
                     /translation="MRKSGIAALGAASLLLTLPLASAAHAKAASVYVQCDGSPNNVSA
                     AETALRIIALTAVIGLLAPPPEQADTSKRVAGAAGVSACTAALEGGETNVMRRMELTL
                     ARAIHRIEAGDHERAIADARSIGGIAGALAQDRGFQRSIALTALEIEAAALLRQGKRS
                     EAELAALRMAALAPYDIANAERASYFLWYSDANLPAKLAFYDNYVKLRPEALISRSQL
                     RAWAGDFRGSARDMESLVGLVRSASAKAADSPTFGFDAQRAVAYTAAGDLAQGDAIAA
                     QTRAAIDRLVAAGKTVGNVESISRADELLQFQKVMRLLAAGDVAGARTAFAARTRWLA
                     PPTPVLESVLVKLRQNLEPALMTGALADDPALLRKRSFDGRVQAVIDEPKNVARLFRS
                     VRPLLKPADFKPAAKSVWRVEGSKYLLPRTTAESASSPFQVIYARDSSGHAAGYALLL
                     HAALLARDRGKQGFVMFPVRKRLEMAIVRFANAGEAGVAPDLIIDAAATINALSPDMP
                     HPGRTK"
     gene            complement(1920..3392)
                     /locus_tag="VF589_01870"
     CDS             complement(1920..3392)
                     /locus_tag="VF589_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8256355.1"
                     /translation="MESPLTSGEQSLEALLRRSAELMAGGRVEEAIAAYERLLARHPD
                     LADSWYNLAWLQRRARRFEAALASYGEALARGVGGAEEVHVNRAVILADHLGRPDAAE
                     AELEAALALNPDYVPALLNLGNLQEDRGQRDAARAAYARALEAEPENALALVRFAGVS
                     QPESPDDPVVARLRQAIGRSADPLGRADLGFALGRALDAAAAYDEAFAAYAAANAASR
                     AASGARYDPKAHERLVDRLIRAFPRAAEPGSAPPAEAPLFICGMFRSGSTLTEQILAA
                     HPAITAGGELDLLPALIASELRPYPEAAAEASPETLDRVRRFYLEGLRAIHPGAGLVT
                     DKRPDNFLYIGLIKTLFPAAKIVHTIRDPLDNILSIYFLHLSPDMAYALDLADAAHWY
                     GQYRRLMAHWHALYPGDIYDADYDALVADPRPAIEALLAFCGLPWDEACLSFHAAANV
                     VKTASVWQVREPLYRRSSGRWRHYAAHLGPLGDLDSRIAR"
     gene            3530..4744
                     /gene="tyrS"
                     /locus_tag="VF589_01875"
     CDS             3530..4744
                     /gene="tyrS"
                     /locus_tag="VF589_01875"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX8256356.1"
                     /translation="MTFQSDLLRLLDERGYIHQLTDAEGLDALAAREIVPGYIGFDAT
                     APSLHVGSLVQIMMLRRLQQAGHKPIVLMGGGTSKIGDPSFKDEARKLIDDDTINANI
                     ASIRRVFGAFLTFGEGPTDAVLVDNADWLDRLEYIPFLREIGQHFSVNRMLSFDSVKL
                     RMEREQSLSFLEFNYMILQGYDFLELSRRAACRLQLGGSDQWGNIVNGIELCRRIDGT
                     QVYGLTTPLMTTADGAKMGKTEKGAVWLNADQLSPYDYWQFWRNAQDADVGKFLRRFT
                     DLPIAEIERLEALEGSEINEAKKVLADAATAMCHGSEAAAEAAETARRTFEEGAAGEA
                     LPTLKVEGEIGLVDALVGLGLSASKNEARRLIAQGGARIDGEAAGEDIVIAVTGEVRI
                     SAGRKKHGLLTR"
BASE COUNT          788 a         1553 c         1720 g          704 t
ORIGIN      
        1 cacgactata aggtcgtgtt cgtcggcgac gcggcaatga gcccctatga gatcacccat
       61 gccggcggat cggtcgagca ttataacgac gaggcggggg cggtctgggt gcagcggatg
      121 gcgaccacct acccctccgc cgcctggctc aacccgacgc ccgagcaata ttgggattat
      181 tcgcattcga cccagctggt gcgccagctg atgcagggcc gcatgtaccc gctgacgctc
      241 gacggcctcg acgacgcgat gcgggcgctg agccgcaaat cctgaccccc tccgcgaacg
      301 gagggggcta agtcatttgg tacggcccgg gtggggcatg tcgggcgaca gcgcattgat
      361 ggtcgcggcc gcatcgatga tcaggtccgg cgcgaccccc gcctcgccgg cattggcgaa
      421 cctcactatc gccatctcga ggcgcttgcg gaccggaaac atgacgaagc cctgcttgcc
      481 gcggtcgcgc gccagcagcg ctgcgtggag cagcagcgca taacccgcgg catggccgga
      541 gctgtcgcgg gcatagatca cttggaacgg ggagctcgcg ctttcggcgg tggtcctcgg
      601 aaggagatat ttggagccct ccacccgcca gaccgacttc gccgccggct tgaaatcggc
      661 cggcttcagc aaaggccgga ccgagcggaa caggcgcgcc acattcttcg gctcatcgat
      721 cacggcctgg acgcggccgt cgaacgagcg cttgcgaagc aaggcgggat cgtcggcgag
      781 cgcaccggtc atcagcgccg gctccagatt ctgccggagc ttgaccagaa ccgattcgag
      841 caccggcgtc ggcggcgcaa gccagcgggt gcgggccgcg aacgccgtcc gtgccccggc
      901 cacgtcgccg gcggcgagga ggcgcatcac cttctggaac tgaagcagct cgtcggcgcg
      961 gctgatcgat tcgacattgc cgaccgtctt gcccgccgcc accagccggt cgatcgcggc
     1021 gcgggtctgg gccgcgatgg cgtcgccctg cgcaaggtcg ccggccgcgg tataggcgac
     1081 tgcccgctgg gcgtcgaaac cgaaggtcgg ggaatcggcg gccttggcgg aggccgaccg
     1141 caccaggccg accagcgatt ccatgtcgcg cgccgacccg cgaaagtccc cggcccaagc
     1201 gcgcaactgt gagcggctga tcagggcctc cgggcgaagc ttcacataat tgtcgtagaa
     1261 agcgagcttc gccggcaggt tggcgtccga ataccagaga aagtagctgg cgcgttcggc
     1321 attggcgatg tcgtagggcg cgagcgcggc catgcgcagc gcggcaagct cggcctcgct
     1381 ccgcttgccc tgccgcagca atgccgcagc ctcgatctcg agcgcggtga gcgcgatgct
     1441 gcgctgaaag ccgcggtcct gcgccagcgc gccggcgatc ccgccgatcg agcgcgcatc
     1501 ggcgatggcg cgctcatgat cgcccgcctc gatgcggtgg atcgccctgg ccaaggtcaa
     1561 ttccatccgg cgcatcacgt tggtttcccc gccttcgagc gcggccgtgc acgcgctgac
     1621 gccggccgcc ccggcgacgc gcttcgaagt gtcggcctgc tcgggcggcg gcgccagcag
     1681 cccgatcacc gccgtcaggg cgatgatccg aagcgcggtt tcggccgccg agacattgtt
     1741 agggctgccg tcgcactgga cgtagaccga tgccgctttg gcatgagccg ccgacgcgag
     1801 cggcaaggtc aacagcagcg aggccgcgcc aagcgcggcg attccagatt ttcgcatttt
     1861 tccccccgga tcgaatccca aggatggatt cgatgctgcc gatcctaata tccgaatctc
     1921 tagcgggcaa tccggctgtc gagatcgccc agcgggccta gatgagcggc gtaatggcgc
     1981 cagcggccgg atgagcggcg gtagagcggc tcgcggacct gccacacgct ggcggtcttc
     2041 acgacgttgg cggcggcgtg gaaggagagg caggcctcgt cccagggcag gccgcaaaag
     2101 gcgagcagcg cctcgatcgc cggccgcgga tcggcaacca gcgcgtcgta atcggcatcg
     2161 taaatgtcgc ccggatagag cgcgtgccag tgcgccatca gccggcggta ctggccgtac
     2221 caatgggcgg cgtcggccag gtcgagggcg taggccatgt ccgggctcag gtgcaggaag
     2281 tagatggaca ggatgttgtc gagcgggtcg cgaatggtgt ggacgatttt cgccgcgggg
     2341 aagagcgtct tgatgaggcc gatgtagagg aaattgtcgg gccgtttgtc ggtaacgagg
     2401 ccggcgccgg gatggatcgc ccgcaggccc tcgagataga aacggcggac ccgatcaagg
     2461 gtctccggcg aggcttcggc ggcggcttcg ggataggggc gcagctcgct ggcgatcagc
     2521 gccggcagca ggtcgagctc gccgccggca gtgatggcgg ggtgggcggc gaggatctgc
     2581 tcggtcagcg tcgagcccga gcggaacatg ccgcaaatga acaagggcgc ttcggcgggc
     2641 ggggccgagc ccggctcggc ggcgcgcggg aaggcgcgga tcagccggtc gacgaggcgc
     2701 tcgtgcgcct tgggatcgta gcgcgcgccg ctggcggcgc ggctggcggc gttggcggcg
     2761 gcataagcgg cgaaggcctc gtcataggcg gcggcggcgt cgagcgcccg gccgagcgcg
     2821 aagccgagat cggcgcggcc gagcgggtcg gcggagcggc cgatcgcctg ccgcaggcgg
     2881 gccacgaccg gatcgtcggg cgattcgggt tggctgacgc cggcgaaccg caccagcgcg
     2941 agcgcgtttt cgggctcggc ctccagcgcg cgcgcataag cggcgcgggc cgcgtcgcgc
     3001 tggcctcggt cctcctggag gttgccgagg ttgaggagcg ccgggacata gtccgggttg
     3061 agcgcgagcg cggcctcgag ctcggcctcg gccgcgtcgg ggcggccgag atggtcggcc
     3121 aggatcaccg cgcggttgac atggacctcc tcggcgccgc cgacgccgcg ggcgagcgcc
     3181 tcgccatagg aagcgagcgc cgcctcgaag cgccgcgcgc ggcgctggag ccaagcgaga
     3241 ttataccagc tgtcggccaa atcggggtgg cgggcgagca gccgctcata ggcggcgatc
     3301 gcctcttcca cccggccgcc ggccatcagc tccgccgagc gccggagcag cgcttcgaga
     3361 ctttgctcgc cacttgtgag cggtgactcc atcatgaact cccttcggcc cgcgccctcg
     3421 gcggccatcg tcgaccggct gtggcgaaag gcgtgggctt cgacaatccc ggatcgggta
     3481 cgggaccgaa cgggcttgtg tcgggcgacg cccatgctaa ccgcgcccga tgaccttcca
     3541 gtccgatctc ctccgcctgc tcgacgaacg cggctatatc caccagctta ccgatgcgga
     3601 ggggctggac gcgctcgccg cgcgcgagat cgtgcccggc tatatcggct tcgacgccac
     3661 cgcgccctcg ctccacgtcg gctcgctggt ccagatcatg atgctgcgcc ggctccagca
     3721 ggccgggcac aagccgatcg tgctgatggg cggcggcacc tccaagatcg gcgacccgag
     3781 cttcaaggac gaagcgcgca aattgatcga cgacgacacg atcaacgcca acatcgcctc
     3841 tatccggcgg gtgttcggcg cgtttctgac cttcggcgag gggccgaccg acgcggtgct
     3901 ggtcgacaat gccgactggc tcgaccggct cgaatatatc ccgttcctgc gcgagatcgg
     3961 ccagcatttc tcggtcaacc ggatgctgag cttcgacagc gtcaaattga ggatggagcg
     4021 cgagcagtcg ctgagcttcc tcgaattcaa ctacatgatc ctccagggat atgatttcct
     4081 ggagctgtcg cgccgcgcgg cctgccggct gcagctcggc ggatcggacc agtggggcaa
     4141 tatcgtcaac ggcatcgagc tgtgccgccg gatcgacggg acgcaggtct atggcctgac
     4201 gacgccgctg atgaccacgg cggacggcgc caagatgggc aagaccgaga agggcgcggt
     4261 gtggctgaac gccgaccagc tctcgcctta cgattattgg caattctggc ggaacgcgca
     4321 ggacgccgac gtcggcaagt tcctgcgccg cttcaccgac ctcccgatcg ccgagatcga
     4381 gcggctggag gcgctggaag gcagcgagat caacgaggcc aagaaggtgc tggccgacgc
     4441 ggcgaccgcg atgtgccacg gctcagaggc cgcggcggaa gcggcggaga cggcgcgccg
     4501 gacgttcgag gagggcgccg ccggcgaagc gctgccgacg ctgaaggtcg agggtgagat
     4561 cgggctggtc gatgcgctgg tcggcctcgg tctcagcgca tcgaagaacg aggcgcggcg
     4621 gctgatcgcc cagggcggcg cgcggatcga cggcgaagcg gctggcgagg acatagtgat
     4681 agccgtgacc ggcgaggttc gtatctccgc cggcaggaag aaacacgggc tgctgacccg
     4741 ataagctcct ccccggaacg gggag
//
LOCUS       DASVRK010000051        10657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_443718, whole genome shotgun sequence.
ACCESSION   DASVRK010000051 DASVRK010000000
VERSION     DASVRK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443718"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VF589_01880"
     CDS             complement(<1..1169)
                     /locus_tag="VF589_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8256357.1"
                     /translation="MSRKSFPYALLATALAAPLQAQPANPATDPEQDEGAPQGDEEEV
                     EEIVVEGRRQRGAVLGDIAPEVQLDQREIRAFGAGNLADLLDALAPQTRSARGRDGGG
                     PVVLLNGRRISGFREIRNMPPEAIERVDILPEEVALKYGYRADQRVVNFVTRRRFRAI
                     TGEVETQFATAGGRSGYEADFNLLRINRNGRWSVDAEFRRDTALYESERDLIQDVSGA
                     PYDLLGNIGAASAESGAEIDPALSALAGEPVTVAGVPASAAAGAPVLGDFVPGANAAN
                     VTDVGRYRTLLPRGDTWSVSGTLNRNILGDVSATVNAGLDGSATTARLGLPEAALLLP
                     AGNPYSPFAGDVTLYRYFEGAGPLTRDSDTLAGHLGVSLTGERERWNWSFTGNFDR"
     gene            1315..1500
                     /locus_tag="VF589_01885"
     CDS             1315..1500
                     /locus_tag="VF589_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256358.1"
                     /translation="MRMYSVQAPAANQPAQCSPAQPWGIHPAMVENACPRCGWVARAM
                     AAPVVPGVPAAARLLAA"
     gene            complement(1525..2331)
                     /locus_tag="VF589_01890"
     CDS             complement(1525..2331)
                     /locus_tag="VF589_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8256359.1"
                     /translation="MRTWVLATMIAAGLAAPAAAQRRQQPYVQPEIRPQYQPPLPSGV
                     PATSAVADLTARIDALEAQLARLTGQAEEDANRVRQLEEEVRRYRGEAEERFGRLERP
                     APASAGPERESAANDVIAPAEGPPAREDGPAGAALTGDPGEDDYLAGFRLWDQGRFAD
                     AAGVLEAMAKKHPKHRRASYARNLAGRAYLDDNKPAAAAKVLLANYQNNPKGERAADS
                     LYFLGQALVRLERPAEACKVYDELEDVYGAGMRDFVRERLPKARTDARCG"
     gene            complement(2417..3256)
                     /locus_tag="VF589_01895"
     CDS             complement(2417..3256)
                     /locus_tag="VF589_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX8256360.1"
                     /translation="MSEADNGLMSSSPIGERLKVAREAQGMSLDDVASRTRIPTRHLQ
                     HIENGEWDALPATTYSVGFARAYGNAVGLNGSAIGVELREQLGGGGPHRKAMAPAHYE
                     PADPARVPPRSLAIVAGLIALLLIVGYAVWRSGAVDDSGVEASDAAGVETILPEAEPL
                     APAAPQAGAAAATGPVVLTATEDVWLRVYEGNGGPRISERTLRAGERFEVPATAKAPQ
                     LITGRPNAIRVTVGAAEIPPLGPPERTVSDVSLLPADLMARAQGAATAPRRDSSCCKR
                     AAP"
     gene            complement(3369..5645)
                     /gene="ptsP"
                     /locus_tag="VF589_01900"
     CDS             complement(3369..5645)
                     /gene="ptsP"
                     /locus_tag="VF589_01900"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX8256361.1"
                     /translation="MPTSSVSSAREILTGLHDVMASRSGAQAKLNKVVKIIAEAMNSE
                     VCSIYLLRDGVLELFATVGLNQSAVHVTKLAMGEGLVGTIAEQVRILNLDEATRHPEF
                     AYRPETGEELFHSFAGVPIVRRERAIGTLCVQHADPRPYDEVEIEALQTVAMVLSELI
                     AGAGLVDKASEAAAAARESGSVRVNGLKLVTGMARGNAVYHQPRILIEHTVAEDIEAE
                     RHRVYSAFRKMREQIDNMTNLAEFGTAGEHHEILQTYKMFAYDEGWSRRINQAIDSGL
                     TAEAAIERVQQRTRMRMREIDDPLLKERMHDLDDLANRLLRIVSGRLGTAAQMGLQTD
                     AILIARNLGPAELLEYDRRRLKGVILEEGSLTAHVTIVARAMGVPVLGRVRDVRQTIV
                     EGDHVLLDVGESVAFVRPTPAMEEAFEGKLLVTQKRRAEFAALRDLPPVTKDGQRISV
                     MVNAGLRDDVAALDVTGADGIGLFRTEFQFLVSAALPRRESQQRLYRSVLDAAGDKPV
                     IFRTIDIGGDKALPYLKIDDGREEDNPAMGWRALRLSLDRDVLMKAQARALLEAAPGR
                     ELNIMFPMVSEPWEFDEAKALIESQRHWLARKKKLLPSQIRYGAMLEVPALAETLDIL
                     LPKIDFLSIGTNDLTQFLFAADRANPRLAERYDWLSPAILRFLRRIVLAAREHEVPVA
                     VCGEMGGRTLEAMALIGIGLRRLSITPVAVGPVKAMIRSLDLGRLAPAIEALLHRPPS
                     DMRSALNAWAEDNDVAIG"
     gene            5850..7892
                     /locus_tag="VF589_01905"
     CDS             5850..7892
                     /locus_tag="VF589_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256362.1"
                     /translation="MATIDGSDDPAFAETIYGSHLADIIRGMLGDDTINGGYGNDVIR
                     GDRGDDAATGGGADIIRGGYNNDQLYGESGDDRLFGDAGNDSLTGGAGADAMYGGSGY
                     DVFFLGSAATMAGDRLDGGSGSDRLFADFSDRSERLVLRAVDPNSSYMAGGVIHITGI
                     ESYELTGTALGDRLSGWRLADSLSGGEGDDLIEGGDGRDFLSGDGGADRLFGGAGSDQ
                     MNGDRLDGPYGDDRLDGGAGGDYMYGHGGEDVLLGGDGNDSMQGGNGEDALNGGLGGD
                     YLHGGAGADVLAGSSGDDILYSHEYGMGPDDNGLEVDQLLGGTGDDLVHIGVGDSADG
                     GTGIDRLALTFGASATGITLVLGQGPVTLANGTSFQGFERLIFQGGAGADDVTAGGLE
                     DSLYGGGGADILRGGASRDIMNGQDGDDLVEGGGGNDMIEDGAGDDSSFGGAGDDSLY
                     GRGGADQLSGDDGNDSLWLHGDGGAADQVAGGDGHDRVYLYGPGSMILDLAKQSKNWG
                     VVANDRFGADIEEFIAGEANDILLGNAAANHFSGGGSDDVLDGRGGDDRLDGGDNADT
                     LTGGLGADTFVLQGPGNDQWPGDIVTDFTRGSDTLEVSLDRFQLGNPSFAAAEIGLEL
                     VIDPEATGSGPMFLFDTDSSELWFDLDGAGGENSPVLIATLEGVTTLALSDFTFVG"
     gene            complement(7905..8948)
                     /locus_tag="VF589_01910"
     CDS             complement(7905..8948)
                     /locus_tag="VF589_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8256363.1"
                     /translation="MSTTPPTTGHDRLRRLMKRGADLLGCEVAIMGGAMSWISERHLV
                     SAISNAGGFGVIACGAMTPDLLDREIAETKLRTERPFGVNLITMHPQLSELIDVCGRH
                     GVGHIVLAGGLPPPGSLDRIKATGAKLICFAPAFALAKKLIRSGVDALVIEGMEAGGH
                     IGPVSTSVLAQEILPPLAAEIPIFVAGGIGHGGLIAGYLEMGAAGVQLGTRFVCAHEC
                     IAHPNFKKAFIRASARDAIPSVQIDPRLPVIPVRALKNREMEAFSAKQREVAALLDVG
                     SVEMAEAQLQIEHYWAGALRRAVIDGDVESGSVMAGQSVGMVKAEQSVADIVAGLIDE
                     AAAALQRRALEPA"
     gene            complement(8945..10183)
                     /locus_tag="VF589_01915"
     CDS             complement(8945..10183)
                     /locus_tag="VF589_01915"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX8256364.1"
                     /translation="MARIVMKFGGTSMAGIERIRHVASLVRREAEAGHEVAVVVSAMA
                     GETDRLIQFCREASSLYDPREYDVVVASGEQVTSGLLAITLQGMGLNARSYMGWQLPI
                     RASGHASALIDDIDAAVLERVFAEGGIAVIPGFQGVAADGSLATLGRGGSDTSAVALA
                     AAVGAGRCDIYTDVDGVYTTDPRIVPRARKLPMVTYEEMLELASVGAKVLQTRSVGLA
                     MRERIPIRVLSSFEDRPGTMVADEDTIRDLSMERQVVSGVAYDKNEARITLGGMPDRP
                     GAAAAIFGALAEANVNVDMIVQSVPRAGERTDLTFTVPQAALAQSVDVLGGLKEEVGF
                     EEMLTDTAITKVSIVGVGMRSNPGVAATMFRTLADRRIDLLAITTSEIKVSVLIPEDY
                     TELAVRVLHTAFGLDQEAEG"
     gene            10319..>10657
                     /gene="ubiG"
                     /locus_tag="VF589_01920"
     CDS             10319..>10657
                     /gene="ubiG"
                     /locus_tag="VF589_01920"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX8256365.1"
                     /translation="MESNSPSTIDPREAAHFGRMAAEWWDPKGSSAMLHQLNPVRLGY
                     IRDQIDQHWGLDECDLKPLRGRRAADVGCGAGLLAEPLARLGADVVAVDPAPENIAAA
                     REHALGQGLEI"
BASE COUNT         1577 a         3561 c         3904 g         1615 t
ORIGIN      
        1 cggtcgaaat tgccggtgaa cgaccagttc cagcgctcgc gctcgccggt cagcgagacg
       61 ccgaggtgac cggccagggt gtcgctgtcg cgggtgagcg ggcctgcgcc ctcgaaatag
      121 cggtagagcg tcacgtcgcc ggcgaacggc gaataggggt tgccggcggg aagcagcagt
      181 gcggcctcgg gcaggccaag ccgcgcggtg gtcgcgctgc cgtctaggcc cgcattcacc
      241 gtcgccgaca catcgccgag gatgttgcgg ttcaaggtgc cgctgaccga ccaggtgtcg
      301 ccgcgcggga ggagggtgcg gtagcgcccg acatcggtga cgttggccgc gttggcgcca
      361 ggaacgaagt cgccgagcac gggcgctccg gcggcggccg aggccgggac gccggcgacg
      421 gtgaccggct ctcccgccaa tgcgctcagc gccgggtcga tctcggcgcc cgactcggcc
      481 gaagccgcgc cgatgttgcc gaggagatcg taaggcgcgc ccgacacgtc ctggatgagg
      541 tcgcgctcgc tctcatagag cgcggtgtcg cggcggaatt cggcgtccac gctccagcgg
      601 ccgttgcggt tgatgcggag caggttgaaa tcggcttcgt agccgctgcg gccgccggcg
      661 gtggcgaact gggtctcgac ctcgccggtg atggcgcgga agcggcggcg ggtgacgaag
      721 ttcaccaccc tctggtcggc gcgatagcca tatttgagcg cgacctcctc gggcaggatg
      781 tcgacgcgct cgatcgcctc gggcggcatg ttgcggatct cgcgaaagcc ggagatgcgg
      841 cggccgttca acaggaccac cgggccgccg ccgtcgcggc cgcgggcgct gcgggtctgc
      901 ggtgcgagcg cgtcgagaag gtcggcgagg ttgccggctc cgaaggcgcg gatctcgcgc
      961 tggtcgagct gcacctcggg tgcgatgtcg ccaagcaccg cgccgcgctg ccggcgcccc
     1021 tcgacgacga tttcctcgac ctcttcctcg tccccttgag gcgcgccctc gtcttgctcg
     1081 ggatcggtgg cggggttcgc cggctgcgcc tggagcggcg cggccagggc ggtggcgagg
     1141 agggcgtagg ggaaggactt tcgggacaag gctcggctca tattgggtga gcgcgcctct
     1201 ggcgtcgcgc ggctgaaccc cgtctgtatc cgcgcgggcg tgacggagcc ggaattaacg
     1261 caaccgaata gcggcaaagc cgttcacgcc ttagcaaaaa aactgatata ggttatgcgt
     1321 atgtacagcg tccaagctcc cgcggcgaac cagcccgccc aatgctcccc ggcccagccc
     1381 tggggcatcc accccgccat ggtcgagaat gcctgcccgc gctgcggctg ggtggcccgt
     1441 gcgatggccg cgcctgtcgt gccgggcgtg ccggcggccg cgcgccttct cgccgcctga
     1501 cggagcgcta agcctagctt cggctcagcc gcaacgggcg tcggtgcgag ccttgggaag
     1561 ccgctcgcgg acgaagtcgc gcatcccggc gccgtagacg tcttccagct cgtcatagac
     1621 cttgcaggcc tcggccgggc gttcgagccg gaccagcgcc tggccgagga aatagaggct
     1681 gtcggcggcg cgctcgccct tggggttgtt ctggtaattg gcgagcagga ccttggccgc
     1741 ggccgccggc ttgttgtcgt cgagataggc gcggccggca aggttgcggg cgtagctggc
     1801 gcggcggtgc ttgggatgct tcttcgccat cgcctccaat acgccggcgg cgtcggcgaa
     1861 gcggccctgg tcccacagcc ggaagccggc cagataatcg tcctcgccgg gatcgccggt
     1921 gagggcggcg ccggccggcc catcctcgcg cgctggcggc ccctcggccg gagcgatgac
     1981 gtcgttggcc gcgctctccc gttccgggcc cgccgaggcc ggggcgggac gttcgagccg
     2041 gccgaaacgc tcttcggcct cgccccggta gcggcgcacc tcctcctcga gctggcggac
     2101 gcggttcgca tcctcctcgg cctggccggt aagccgcgcc agttgcgctt cgagcgcgtc
     2161 gatccgggcg gtgaggtcgg cgaccgcgct ggtggcgggc acacccgaag ggagcggcgg
     2221 ctgatattgg gggcggatct cgggctgcac ataaggctgc tggcggcgct gcgccgcggc
     2281 cggcgcggcc agcccggccg cgatcatggt ggcgagaacc caggtacgca tcgacaatcc
     2341 cccgacagaa caagccgcag catattaggc ggccgcgtct gtcgccagca ttaagcgcgc
     2401 gtaccgtgtc gctcgttcac ggcgcggcgc gtttgcagca cgacgagtct ctgcgcggcg
     2461 cggtcgcggc tccctgcgcc cgcgccatca ggtcggcggg caggaggctg acgtcggaga
     2521 cggtgcgctc cggcggcccg agcggtggga tttcggcggc gccgacggtg acccggatcg
     2581 cgttgggacg gccggtgatg agctgcggcg ccttggcggt cgccggcact tcgaaccgct
     2641 cgccggcgcg cagggtgcgc tcggagatgc gcgggccgcc attgccttcg tagacgcgca
     2701 gccagacatc ctcggtcgcg gtcagcacca ccgggccggt ggccgccgcc gcgccggcct
     2761 ggggcgcggc cggagcgagg ggctcggcct cgggaaggat cgtctcgacg ccggccgcat
     2821 cgctggcctc gacgcctgaa tcgtcgaccg cgccgctgcg ccaaacggca tagccgacga
     2881 tcagcagcaa ggcgatcagc ccggcgacga tggcgagcga tcgcggcggc acgcgggccg
     2941 ggtcggccgg ctcgtaatga gcgggcgcca tggccttgcg gtgggggccg ccgccaccga
     3001 gctgctcgcg cagctcgacg ccgatcgcgc tgccgttgag gccgacggca ttgccataag
     3061 cgcgggcgaa gccgacgctg taggtggtcg ccggcagcgc atcccactcg ccattctcga
     3121 tatgctgcag gtggcgggtc gggatccggg tgcggctggc gacgtcgtcg aggctcatgc
     3181 cctgggcctc gcgcgcgacc ttgagccttt ccccgatcgg actggaagac atcaggccgt
     3241 tatccgcttc actcattcaa ctacccccgc gccggccaat cccggctatc ggcccgggca
     3301 tgacatagca cgggcccctc cttgtcaacc acgcccgggg ccgtccgccg ccggcgcggg
     3361 ccggcccgtc agccgatcgc cacgtcattg tcttcggccc aggcattgag cgccgagcgc
     3421 atgtccgacg gcggccgatg gagcaacgcc tcgatcgccg gcgccagccg cccgaggtcg
     3481 agcgagcgga tcatcgcctt gaccgggccg accgcgaccg gggtgatcga cagccgcctc
     3541 aggccgatgc cgatcagcgc catcgcctcg agcgtgcgcc cgcccatctc gccgcacacc
     3601 gcgaccggca cctcatgttc gcgggcggcg agcacgatcc gccggaggaa gcgcaggatg
     3661 gcggggctga gccagtcata gcgctcggcc agccgcggat tggcgcggtc ggcggcgaac
     3721 aggaactggg tgaggtcgtt ggtgccgatc gacaggaagt cgatcttcgg caggaggata
     3781 tcgagcgtct cggcgagcgc cggcacttcg agcatcgcgc catagcgtat ctgcgaggga
     3841 agcagcttct tcttcctggc gagccagtgg cgctggcttt cgatcaaggc tttcgcttcg
     3901 tcgaactccc agggctcgga caccatcggg aacatgatgt tgagctcgcg gcccggcgcg
     3961 gcctcgagca gggcccgggc ctgcgccttc atcagcacgt cgcggtcgag cgacagccgt
     4021 agcgcccgcc accccatcgc cggattgtcc tcttcgcggc cgtcgtcgat cttgaggtag
     4081 gggagcgcct tgtcgccgcc gatatcgatg gtgcggaaga tgaccggctt gtcgccggcc
     4141 gcgtcgagca ccgagcgata caggcgctgc tggctttcgc ggcgcgggag ggcggccgag
     4201 accaggaact ggaattcggt gcggaacagg ccgatgccgt cggcgccggt gacgtcgagg
     4261 gcggcgacgt cgtcgcgcag gccggcattg accatcaccg agatgcgctg gccgtccttg
     4321 gtcaccggcg gcaggtcgcg gagggcggcg aattccgccc gccgcttctg ggtgacgagg
     4381 agcttgccct cgaacgcctc ctccatcgcc ggggtcgggc ggacgaaggc gaccgactcg
     4441 ccgacgtcga gcaggacatg atcgccctcg acgatcgtct ggcgaacgtc gcggacgcgg
     4501 cccagcaccg gcacgcccat ggcgcgggcg acgatggtga cgtgggcggt cagcgagcct
     4561 tcttcgagga tgacgccctt gaggcggcgg cggtcatatt cgagcaattc ggccgggccg
     4621 aggttgcggg cgatcaagat ggcgtcggtc tggaggccca tctgggcggc ggtgccgagc
     4681 cgtccggaca cgatgcggag cagccggttg gcgagatcgt cgaggtcgtg catccgctcc
     4741 ttgagcaggg ggtcgtcgat ctcgcgcatc cgcatccggg tgcgctgctg gacgcgctcg
     4801 atcgccgcct cggcggtgag gccgctgtcg atcgcctggt tgatccgcct cgaccagccc
     4861 tcgtcatagg cgaacatctt gtaggtctgg aggatctcgt gatgctcgcc ggcggtgccg
     4921 aactcggcga ggttggtcat gttgtcgatc tgctcgcgca tcttgcggaa cgcggaatag
     4981 acgcggtggc gctcggcttc gatatcctcg gcgacggtat gctcgatcag gatgcgcggc
     5041 tggtggtaga ccgcgttgcc gcgcgccatg ccggtgacca gcttgaggcc attgacccgc
     5101 accgagccgg attcccgcgc cgcggcggcg gcctcgctgg ccttgtcgac caggccggcg
     5161 ccggcgatca gctcggacag caccatcgcc accgtctgca gcgcctcgat ctcgacctcg
     5221 tcataggggc gcgggtcggc atgctggacg cagagcgtgc cgatcgcgcg ctcgcgccgc
     5281 acgatgggaa cgccggcgaa gctgtggaac agctcctcgc cggtctcggg gcgataggcg
     5341 aattcggggt ggcgggtggc ctcgtcgagg ttgaggatcc gcacctgctc ggcgatggtg
     5401 ccgaccagcc cctcgcccat ggcgagcttg gtgacgtgaa ccgcgctctg gttgaggccg
     5461 accgtggcga acagctcgag cacgccgtcg cgcagcagat agatcgagca gacttcgctg
     5521 ttcatcgcct cggcgatgat cttcaccacc ttgttcaatt tggcctgggc accggaacgc
     5581 gaggccatca cgtcgtggag gccggtcaag atttcgcgcg ccgagcttac cgaactggtg
     5641 ggcatggacc ggcgctatca gaaaggcccg acggttccaa tggcgcccat gccgggcccg
     5701 cgccggacgg ccaaagcgac tgatcgcgcg accaatttcc acggccgccc gcttcgtcgc
     5761 cgcgccgtcc gggctggtcg aaaaatcctc ctgccggcgg cgcgtcgacg tcaggaagcc
     5821 gcccgcaccg acctggagga aatgcggaca tggcgactat cgacggcagc gacgacccgg
     5881 cttttgccga gaccatctac ggcagccacc tcgccgacat catccgcggc atgctcggcg
     5941 acgacaccat caacggcggc tatggcaacg acgtgatccg cggcgaccgc ggcgacgacg
     6001 ccgcgaccgg cggcggcgcc gacatcatcc gcggcggcta taataacgac cagctttacg
     6061 gcgaatccgg cgacgaccgc ctgttcggcg atgccggcaa cgacagcctg accggcggcg
     6121 ccggcgccga cgccatgtac ggcggcagcg gctatgacgt cttcttcctc ggctcggccg
     6181 ccaccatggc gggagaccgg ctggacggcg gatcgggaag cgaccggctg ttcgccgact
     6241 tttcggaccg gtcggagcgg ctggtgttga gggcggtcga tccgaacagc tcctacatgg
     6301 ccggcggcgt cattcacatc accggcatcg aatcctacga gcttaccggc acggcactgg
     6361 gcgaccggct cagcggctgg cggctggcgg actcgctctc gggcggcgag ggcgacgacc
     6421 tgatcgaagg cggcgacggc cgtgacttcc tgtccggcga cggcggcgcg gaccggctgt
     6481 tcggcggcgc cggcagcgac cagatgaacg gcgaccgatt ggatgggccc tatggcgacg
     6541 accggctcga tggcggcgcc ggcggcgatt atatgtacgg ccatggcggc gaggacgtcc
     6601 tgcttggcgg cgacggcaac gacagcatgc agggcggcaa tggcgaggac gcgctcaacg
     6661 gcggcctcgg cggcgactat ctccacggcg gcgccggcgc cgacgtcctt gccggcagct
     6721 cgggcgacga cattctctat tcgcacgaat atggaatggg tcccgacgac aacggcctgg
     6781 aggtcgacca gctcctcggc ggaacgggcg acgacctcgt ccatatcggc gtcggcgaca
     6841 gtgccgatgg cgggaccggc atcgaccggc tggcgctgac cttcggcgct tcggcgacgg
     6901 gcatcaccct ggtcctcggc cagggtccgg tgacgctggc caacggcacc agcttccaag
     6961 gcttcgagcg gctcatcttc cagggcggcg ccggcgccga cgacgtcacc gccggcgggt
     7021 tggaggacag cctgtacggc ggcggcggcg cggacatcct ccgcggcggc gcctcgcgcg
     7081 acatcatgaa cggccaggac ggcgacgatc tggtcgaggg cggcggcggc aacgacatga
     7141 tcgaggacgg cgccggcgac gacagtagct tcggcggcgc cggcgacgac agcctctacg
     7201 gccgcggcgg cgccgaccag ttgtccggcg atgacggcaa cgacagcttg tggctgcacg
     7261 gcgacggcgg cgcggcggac caggtggccg gcggcgacgg ccatgaccgc gtctacctct
     7321 acgggcccgg ttcgatgatc ctcgaccttg ccaagcaatc gaagaattgg ggcgtggtgg
     7381 ccaacgaccg gttcggcgcc gacatcgagg agttcatcgc gggcgaggcc aacgacatcc
     7441 tgctcggcaa cgccgccgcc aaccatttct ccggcggcgg ttccgacgac gtgctcgacg
     7501 ggcgcggcgg cgacgaccgg ctcgacggcg gcgacaatgc cgacacgctg accggcggcc
     7561 tgggcgccga cacgttcgtg ctccagggcc ccggcaacga ccaatggccg ggcgatatcg
     7621 tgaccgattt cacccgcggg tccgacacgc tcgaagtgtc gctggaccgg ttccagctgg
     7681 gcaacccctc cttcgcggcc gcggagatag gcctggagct cgttatcgat ccggaggcga
     7741 cgggcagcgg gccgatgttc ctgttcgaca ccgattcctc ggaattgtgg ttcgacctcg
     7801 acggcgcggg cggggagaat tcgccggtgc tgatcgcgac gctggagggg gtgacgacgc
     7861 tggcgctgag cgacttcacg ttcgtcggct gagccggccc ggcgtcaggc cggctccagc
     7921 gcccgccgtt ggagcgcggc cgcggcttcg tcgatcaggc cggcgacgat gtcggcgacc
     7981 gattgctcgg ccttgaccat gccgaccgac tggcccgcca tcaccgagcc gctctcgacg
     8041 tcgccgtcga tgaccgcgcg gcgcagcgcg cccgcccaat aatgctcgat ctggagctgg
     8101 gcctcggcca tttcgaccga gccgacgtcg agcaaagcgg cgacttcgcg ctgcttggcg
     8161 gagaaagctt ccatctcgcg gttcttcaag gcccggaccg ggatgaccgg gaggcgcggg
     8221 tcgatctgga ccgacgggat ggcatcgcga gccgaggcgc ggatgaacgc cttcttgaaa
     8281 ttgggatggg cgatgcattc gtgggcgcag acgaagcggg tgccgagctg gaccccggcc
     8341 gcgcccattt cgaggtagcc ggcgatgagg ccgccatggc cgataccgcc ggcgacgaag
     8401 atcgggattt cggcggcgag cggcggcaat atctcctggg cgagcaccga cgtcgagacc
     8461 gggccgatat gcccgcccgc ctccatcccc tcgatgacga gcgcgtcgac gccggagcgg
     8521 atcagcttct tcgccagcgc gaacgcgggg gcgaagcaga tcagcttggc gccggtcgcc
     8581 ttgatgcggt cgagcgagcc gggcgggggc agcccgccgg cgagcacgat gtggccgacg
     8641 ccgtggcggc cgcacacgtc gatgagctcg ctcaattgcg gatgcatggt gatgaggttg
     8701 acgccgaacg ggcgctcggt gcggagcttc gtttcggcga tttcgcggtc gagcaggtcg
     8761 ggcgtcatcg ccccgcaggc gatcacgccg aagccgccgg cgttggagat ggcggagacc
     8821 aggtggcgct ccgagatcca gctcatcgcg ccgcccatga tggcgacctc gcagccgagc
     8881 aaatcggcgc cgcgcttcat cagccggcgg aggcggtcgt ggccggtggt ggggggggtg
     8941 gtgctcatcc ctccgcctcc tggtcgaggc cgaaggcggt gtggaggacg cggacggcga
     9001 gttcggtata gtcttcgggg atcagcaccg agaccttgat ctcgctggtg gtgatggcga
     9061 ggaggtcgat gcggcggtcg gccagcgtcc ggaacatggt cgcggcgacg cccgggttgc
     9121 tgcgcatgcc gacgccgacg atagagacct tggtgatggc ggtgtcggtc agcatctcct
     9181 cgaagccgac ctcctccttg agcccgccga gcacgtcgac cgactgggcg agcgcggcct
     9241 gggggacggt gaaggtgagg tcggtgcgct cgccggcgcg gggaacgctc tggacgatca
     9301 tgtcgacgtt gacgttggcc tcggcgagcg cgccgaatat cgcggccgcg gcgcccgggc
     9361 ggtcgggcat gccgccgagc gtgatccgcg cctcgttctt gtcgtaggcg acgccgctga
     9421 cgacctgccg ttccatgctc aaatccctga ttgtgtcttc gtcggccacc atcgtcccgg
     9481 gccggtcctc gaacgaggag agcaccctta tgggtatccg ctcccgcatc gccagaccta
     9541 cagaacgggt ctgcaggact ttggctccga cgctggcgag ctcgagcatc tcctcatagg
     9601 tgaccatcgg cagcttgcgc gcccgcggca cgatgcgcgg atcggtggtg tagaccccgt
     9661 cgacgtcggt gtagatgtcg cagcggccgg cgcctaccgc ggccgccagc gcgacggccg
     9721 aagtgtcgga gccgccgcgg ccgagggtgg cgaggctgcc gtcggcggcg acgccctgga
     9781 agcccgggat gacggcgatg ccgccctccg cgaacacccg ctccagcacg gcggcgtcga
     9841 tgtcgtcgat cagcgccgag gcatggccgg aagcgcggat cggaagctgc cagcccatat
     9901 aggagcgggc gttgaggccc atgccctgga gggtgatggc gagcaggccg ctggtgacct
     9961 gctcgcccga ggcgacgacc acgtcatatt cgcgcggatc gtagagcgag gaggcttcgc
    10021 ggcagaattg aatgaggcgg tcggtttcgc cggccatggc cgagacgacc accgcgacct
    10081 cgtgcccggc ctcggcctcg cgccggacca gcgacgcgac gtggcggatg cgctcgatcc
    10141 cggccatcga ggtgccgccg aatttcatca cgatgcgggc catgcgaggg ctgatagggg
    10201 gggctcggag cgagggcaac gctccgttcg tcctgagctt gtcgaaggac tgcttttcct
    10261 gccgtccaga gaagggcagg gcttcgacaa gctcagcccg aacggttaga gtcgagggat
    10321 ggagtccaac tcgcccagca cgatcgatcc cagggaagcc gcgcatttcg ggcggatggc
    10381 ggcggaatgg tgggacccga agggctcctc ggccatgctc caccagctca acccggtgcg
    10441 gctcggctat attcgcgacc agatcgacca gcattggggg ctggacgaat gcgatctgaa
    10501 gccgctcagg ggccgccgcg ccgccgatgt cggctgcggc gccgggctgc tcgccgagcc
    10561 gctcgcgcgg ctcggcgccg atgtggtggc ggtcgacccg gcgcccgaga atatcgcggc
    10621 ggcgcgcgag catgcgctcg gccaggggct cgagatc
//
LOCUS       DASVRK010000052         7064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_506568, whole genome shotgun sequence.
ACCESSION   DASVRK010000052 DASVRK010000000
VERSION     DASVRK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506568"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VF589_01925"
     CDS             complement(<1..1220)
                     /locus_tag="VF589_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8256366.1"
                     /translation="MTKARFDTRTLLLATAAAAAFASAAPAAAQVQEEQQAQPDPVAA
                     ATAEDDALTDQEIVVTAQKRAEDQQDVPISISVVSGQELVETGATQLTEIAGYVPGLH
                     VSNLGSPGQTQVSLRGIAPLAAGASVGTYVDDAPVGSSTVYTESAGFTVDLLPYDLAR
                     IEVLRGPQGTLYGASTIGGLLKYVTITPDLNDFSARAGVEGFNIRNAGEIGYAFQAFV
                     NVPIVTDRLAVTASFAHRKTPGWIDNVQTGDSDQNGFDQTGFRASLLWRPVDELSVRL
                     SAMHQEIDSESSTITAQNLNGVDIGNGRSNFNFVAEPFTSKFRNYAASIDYDFGFASL
                     SSTTTYTDAERREVVDASRIFGVLFPLLTGGAIGPGITPFDNSITMEKVTQEIRLTSA
                     SGGRFEWLLGFIYT"
     gene            complement(1335..2669)
                     /locus_tag="VF589_01930"
     CDS             complement(1335..2669)
                     /locus_tag="VF589_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX8256367.1"
                     /translation="MIEERAPTWFGQPRGLTILFLTNMWEQFSYFGMRALLVYYMTTT
                     LLFDQEKASSIYGFYTAFAYFTPIIGGTIADRWLGKRKAVIIGASVMAAGHFMMAFEP
                     AFYAALAVIALGNGLFLPTLPSQINDLYRADDPRRPWAYNVYYVGVNVGAFLAPLVCG
                     FLGETYGWHYGFGAAGVGMLAGLLIYLLGQRHQPAKQALAQPVLPAGSDSRRGRDTLL
                     LLLGIGLAVTVFRAAYEQIGNTFALWMRDDVDRVIAGAEVAAAMFFSLNPLLVMVMTP
                     LLLARWKRQAERGRELSVMHKMACGALLVAASYLLVAAAETVSGDAPAHWLWLLSFFL
                     VFTLGELYILPNGLGIFARLAPPKLGASTVAAWYFAIFTGSLAAGQIGRLWSRVDHSS
                     FFILLAAIATGAAVLLYLLDRPTRRVLAGAAEQPGVDDLTDVPDGLARPRHV"
     gene            complement(2666..3673)
                     /locus_tag="VF589_01935"
     CDS             complement(2666..3673)
                     /locus_tag="VF589_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX8256368.1"
                     /translation="MSIELRYEVETRKLAAPFRISGYVFESTDLVVVTLSDGEHRGRG
                     EAAGVYYLDDEAANIAATLDAKRDAIEGCESREALRHILPAGGARNAVDCAMWELEAS
                     RAGVPAWKLAGLDSVRPLVTTFTIGADEPATMADKARAFVGAKSIKIKLTGDLELDIA
                     RVAAIRAVRPDVWIGVDANQGFSIAELGPLAAAMVEQRVSLIEQPLARGKEADLTGYD
                     GPIPLAADESALGLSDVAGLVGRFQVLNIKLDKCGGLTEALLMAEAARRLGLGVMVGN
                     MTGTSLAMAPAFIVGQYCEVVDLDGPIFMAEDRTPSVDYADGTIWCPDEVWGSGHPVP
                     R"
     gene            complement(3673..5673)
                     /locus_tag="VF589_01940"
     CDS             complement(3673..5673)
                     /locus_tag="VF589_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8256369.1"
                     /translation="MRKALSFLAIVGAALALPLAAQSLRPLSPTAAQPMSPAEAVQGN
                     APTAPGQVLGAPLTAADVNAWLDGFMPYALARGDIPGAVVVVVRDGQVLAQRGYGYAD
                     LATRKRIDPRTTLFRPGSISKLFTWTAVMQQVEQGKLNLDADVNQYLDFKIPPYRGKP
                     VTLRNIMTHTAGFEEQVKDIITTGDNEALPYEQLLKRWVPSRVYAPGITPAYSNYATS
                     LAGYIVQRVSGEPFNQYIERHIFAPLGMTRSSMRQPLPAHLKPLMANGYSSGADEPVG
                     FEFVGPAPAGSLSASGEDMGRFMIAHLQNGRGILQPQTARLMHSRASHPIPGLNGMTL
                     GFYEHNINGRRVIAHGGDTNAFHSDLHLFPAEGTGLYLSFNSGGKEGTSTSLRQAIFE
                     QFADRYFPAGPAAQPTVDAETARRNAELLVGTWSSSRRAFSSFVSIADLLGQTRISVG
                     EDGQLIAPVAEVLGLRPFKWVPAGPMLWRDANGHEMLGAKVENGKAVQLSINAIAPIM
                     VMLPVPWYLNSAWLLPLLYASMAILALTLILWPTRALVRRRFGAALPLEPRDLRAYRW
                     SRVAAAAILGVLVAWFTAVTMMFADVSFGSAMDAIILTLGVLSLVAFVGGFLVLLWYA
                     YTVWRGKWRWTAKAWSVLLVIASATVLHVAINYNLIGFSTNY"
     gene            5796..6506
                     /locus_tag="VF589_01945"
     CDS             5796..6506
                     /locus_tag="VF589_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX8256370.1"
                     /translation="MKSAGSTRAGDQPTLGAVIRNLRAAKGWTLREMSERTDIPMSTL
                     SKVEHDRLTLTYDKLLQLSEKLDIRIADLFTGADAPSDPGVTGRRSIGRIDEAVRVET
                     PNYDYYYLCTDLRRKRMVPILTRIRAKSLSEFGDLISHPGEEYIHVLEGAIVVHTEFY
                     DPVTLKTGEAIYIDSNMGHAYVAAEECEEALVLGVCSSDDHALMSSLMQMHEPLHAGR
                     STPDALGTKVRSLRRPAA"
     gene            complement(6503..>7064)
                     /locus_tag="VF589_01950"
     CDS             complement(6503..>7064)
                     /locus_tag="VF589_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8256371.1"
                     /translation="ELERMILLEGHRQLAEVGFSRFSAREVAKRIGYSIGTLYNVFGT
                     HDRLILAINTRTFSLWADHLRAGLEGREGDRIRALVEGYFSFARANPNLWMAIYDHRL
                     PPGAAMPEEYDAERGELTRIVTEEIAAALPEAARAGAPRLARSLIATVHGHCTFALNG
                     TFALMGEAEPVELALARVAESLAAAG"
BASE COUNT         1215 a         2261 c         2457 g         1131 t
ORIGIN      
        1 tcggtgtaga tgaagccgag cagccattcg aaccggccgc ccgacgccga cgtcagccgg
       61 atttcctgcg tcactttctc catcgtgatc gaattgtcga acggcgtgat gccggggccg
      121 atggcgccac cggtcaggag cgggaacagc acgccgaaga tgcggctggc gtccaccacc
      181 tcgcggcgct cggcatcggt ataggtcgtc gtcgaggaga gggacgcaaa gccgaagtcg
      241 taatcgatcg aggccgcgta attccggaat ttcgaagtga acggctcggc cacgaaattg
      301 aagttggagc ggccgttgcc gatgtcgacg ccgttcagat tctgggcggt gatcgtgctg
      361 ctctcggagt cgatctcctg gtgcatcgcg gacaggcgga cgctcaattc gtccaccggc
      421 cgccacagca ggctggcccg gaagccggtc tggtcgaaac cgttctgatc gctgtcgccg
      481 gtctggacat tgtcgatcca gcccggcgtc ttgcgatgcg cgaagctcgc ggtgacggcc
      541 agccggtcgg tgacgatcgg cacgtttacg aacgcctgga aggcatagcc gatctcgccc
      601 gcattgcgga tgttgaagcc ttcgacgccg gcgcgcgcgc tgaaatcgtt gaggtcgggc
      661 gtgatggtga catatttgag cagcccgccg atggtgctgg cgccgtaaag cgtgccttgc
      721 ggcccgcgca gcacttcgat ccgcgccaga tcgtagggca ggagatcgac ggtgaagccg
      781 gcgctttccg tatagaccgt gctcgacccg accggagcat cgtcgacata ggtgcccacg
      841 ctggcgccgg ccgcgagcgg ggcgatgccg cgcagcgaca cctgggtctg gcccggcgag
      901 ccgagattgc tgacatggag gcccggaacg taaccggcga tctcggtaag ctgggtggcg
      961 ccggtctcga ccagctcctg gccgctgacc acgctgatcg agatcggcac gtcctgctgg
     1021 tcttcggcgc gcttttgggc ggtgacgacg atctcctggt cggtcagggc atcgtcctcg
     1081 gccgtcgcgg ccgcgaccgg gtccggctgc gcttgctgct cctcctgcac ctgggcagcg
     1141 gccggtgcgg ctgaagcgaa ggcggccgcc gctgccgttg cgagcaagag ggtgcgggta
     1201 tcgaatcttg ccttggtcat gatgcttccc tttgtcggac cgcctgcgcg ccacccgtca
     1261 ggcccctgtt gcgcttggtc ggcgccggaa agtcgtctcc gggcgccgct tcgaggttcg
     1321 tgtcgtttcg cgtttcagac atgccggggc ctcgcgaggc cgtcgggcac atccgtcaga
     1381 tcgtcgacgc cgggctgctc cgccgcgccg gcgagcacgc gcctggtcgg ccggtcgagc
     1441 aggtagagca ggaccgccgc accggtcgcg atcgccgcaa gcaggatgaa gaaagagctg
     1501 tggtcgacgc ggctccacag ccgcccgatc tgccccgccg cgaggctccc ggtgaagatc
     1561 gcgaaatacc aggcggcgac cgtgctggcg ccgagcttcg gcggcgcgag gcgggcaaag
     1621 atgccgagcc cgttcggcag gatgtagagc tcgccgagcg tgaagacgag gaagaaggac
     1681 agcagccaca gccagtgcgc cggcgcgtcg ccgctcacgg tttcggcggc ggcgacgagc
     1741 agataggatg cagcgacgag caaggcgccg cacgccatct tgtgcatcac cgaaagctcg
     1801 cgcccgcgct ccgcctgccg cttccagcgg gcgagcagca aaggcgtcat caccatcacc
     1861 agcaaagggt tcagcgagaa gaacatcgcc gccgcgactt cggcgccggc gatgacccga
     1921 tcgacgtcgt cgcgcatcca caaggcgaac gtgttgccga tctgctcgta ggcggcgcgg
     1981 aaaactgtga cggcaaggcc gatcccgagc agcagcagca aggtgtcgcg gccgcggcgc
     2041 gaatccgaac cggccggcag gaccggctgg gcgagcgcct gtttggccgg ctggtggcgc
     2101 tgtccgagca ggtagatcag caggccggcg agcatgccga cgccggccgc gccgaagccg
     2161 taatgccagc cataggtctc gccgaggaag ccgcagacca gcggcgccag gaaggcgccg
     2221 acattcacgc cgacataata gacgttatag gcccagggcc gccgcggatc gtcggcgcgg
     2281 taaaggtcgt tgatctggct cggcagggtc gggaggaaca ggccgttgcc gagcgcgatc
     2341 accgccagcg cggcgtagaa ggcaggctcg aacgccatca tgaaatggcc cgccgccatc
     2401 acgctggcgc cgatgatcac cgccttacgc ttgcccagcc agcggtcggc gatggtgccg
     2461 ccgatgatcg gcgtgaaata ggcgaaggcg gtgtagaagc cgtagatgct cgacgccttc
     2521 tcctggtcga acagcagcgt cgtcgtcatg taatagacga gcaaagcgcg catgccgaaa
     2581 taggagaatt gctcccacat gttggtgagg aacaggatgg tcagcccgcg cggctgaccg
     2641 aaccaggtcg gcgcgcgctc ctcgatcatc gcggcaccgg atggcccgag ccccagacct
     2701 cgtccgggca ccagatcgtg ccgtcggcat agtcgacgct gggggtgcgg tcctcggcca
     2761 tgaagatcgg gccgtcgagg tcgaccacct cgcaatattg gccgacgatg aaggcgggcg
     2821 ccatggcgag gctggtgccg gtcatgttgc cgaccatcac gccgagcccg agccggcgcg
     2881 ccgcttcggc catgagcagg gcctcggtaa ggccgccgca cttgtcgagc ttgatgttga
     2941 gcacctggaa ccgccccacc agcccggcaa cgtcgctcag accaagcgcg ctttcgtcgg
     3001 cggcgagcgg gatcgggccg tcatatccgg tgagatcggc ttccttgccg cgggcgagcg
     3061 gctgctcgat cagcgagacc cgctgctcga ccatcgccgc ggccagcgga cccagctcgg
     3121 cgatcgagaa gccctggttg gcgtcgacgc cgatccacac gtcgggacgg acggcgcgga
     3181 ttgccgccac ccgcgcaatg tccagctcga ggtcgccggt cagcttgatc ttgatcgatt
     3241 tggccccgac gaaagcgcgc gctttgtcag ccatggtcgc cggctcgtcg gcgccgatgg
     3301 tgaatgtggt caccagcggt cggaccgagt ccagcccggc gagcttccaa gccgggacgc
     3361 cggcgcggga cgcctccagc tcccacatcg cacagtcgac cgcattgcgg gcgccgcccg
     3421 ccggcaggat gtggcgcagc gcctcgcggc tttcgcaacc ctcgatcgcg tcgcgcttgg
     3481 cgtctaaagt ggcggcgatg ttggcggctt cgtcgtctag ataatagacg ccggccgctt
     3541 cgccgcggcc gcgatgctcc ccgtcgctta gcgtgacgac gaccaggtcg gtgctttcga
     3601 acacatagcc ggagatgcgg aacggcgccg ccagcttgcg tgtctccacc tcatatcgca
     3661 gctcgatcga catcagtagt tggtcgaaaa gccgatgagg ttgtagttga tcgcgacgtg
     3721 gaggacggtc gccgaggcga tcaccagcag gacgctccac gccttcgcgg tccagcgcca
     3781 ctttccgcgc cagacggtgt aagcatacca gagcaggacc aggaacccac cgacgaaggc
     3841 gaccaggctc agcacgccga gcgtcaggat gatggcgtcc atggcgctgc cgaagctgac
     3901 gtccgcgaac atcatcgtca ccgcggtaaa ccaggcgacg agcactccca ggattgcggc
     3961 agcggcgacc cggctccagc gataggcgcg aaggtcgcgc ggttcgagcg gcagggcggc
     4021 cccgaaccgg cgccggacaa gcgcgcgcgt cggccacagg atcaaggtca gagcgagaat
     4081 ggccatgctg gcgtagagca atggcagcag ccaggccgag ttgaggtacc acggcaccgg
     4141 cagcatcacc atgatcgggg cgatggcgtt gatgctgagc tgcaccgcct tgccgttctc
     4201 gaccttggcg ccgagcatct cgtgcccgtt ggcgtcgcgc cacagcatcg ggccggcggg
     4261 cacccattta aacggacgca ggccgagcac ttcggcgacg ggcgcgatca gctgcccgtc
     4321 ctcgccgacg ctgatccggg tctggccgag caggtcggcg atcgacacga agctggaaaa
     4381 ggcgcggcgc gaactgctcc acgtgccgac cagcagctcg gcgttgcgcc gggcggtctc
     4441 ggcgtcgacg gtcggctgag cggccggccc tgccgggaag tagcggtcgg cgaattgttc
     4501 gaaaatggcc tggcggagcg acgtcgaggt gccctccttg ccgccactgt tgaacgacag
     4561 gtagagcccg gtgccctcgg ccgggaacag gtgaaggtcg ctgtgaaagg cgttggtgtc
     4621 cccgccatgc gcgatcaccc tccggccgtt gatgttatgc tcgtaaaagc cgagcgtcat
     4681 cccgttcagc cccgggatcg gatggctggc gcggctgtgc atcagccggg cggtctgcgg
     4741 ctggaggatg ccgcggccgt tctgcagatg ggcgatcatg aagcgcccca tgtcctcgcc
     4801 gctggcggac aggctgccgg ccggcgccgg cccgacgaac tcgaagccga ccggctcgtc
     4861 cgcgcccgag gaatagccgt tggccatcag cggcttcaga tgcgccggca gcggctggcg
     4921 catgctcgag cgggtcatgc cgagcggcgc gaagatgtgg cgctcgatat attggttgaa
     4981 cggctcgccc gagacgcgct gcacgatgta gcccgccagc gaggtcgcgt aattggaata
     5041 agccggagtg atgcccggag catagacgcg gctcggcacc cagcgcttca gcagctgctc
     5101 gtaaggcaga gcctcattat cgccggtggt gatgatgtcc ttgacctgct cctcgaagcc
     5161 ggccgtgtgg gtcatgatgt tgcgcaaagt gaccggcttg ccccggtagg gcgggatctt
     5221 gaaatcgaga tactggttga cgtcggcgtc gaggttgagc ttgccctgct cgacctgctg
     5281 catgacggcg gtccaggtga acaatttgga gatcgagcca ggccggaaca gggtggtgcg
     5341 cgggtcgatg cgcttgcggg tggcgaggtc ggcatagcca tagccgcgct gcgcgagcac
     5401 ttggccgtcg cggacgacga cgacgacggc gcccggtatg tcgccgcggg cgagggcgta
     5461 aggcatgaag ccgtcgagcc aggcgttgac gtcggcggcc gtgagcggcg cgcccagcac
     5521 ctgtccgggc gcggtcggtg cgttgccctg gaccgcctcc gccggcgaca tcggctgggc
     5581 ggcggtcggg ctgagcggcc tcagcgattg ggcggccagc gggagggcca gcgcagcccc
     5641 gacgatcgcc aggaatgaca gtgccttgcg cattctcgcc gagctcccct tctcgtctgc
     5701 atatttgctg ctaatgtcgt tttacgttat cttttagcag cagcaggacg gatgatccta
     5761 atcaggacat aatgctccta attggaaaat gtcaaatgaa gagtgccggt tcgacacgcg
     5821 caggcgatca gcccaccctg ggagcggtga tccgcaacct gcgggcggcg aagggctgga
     5881 cgctgcggga gatgagcgag cgcaccgaca ttccgatgtc gacgctctcg aaggtggagc
     5941 atgaccggct caccctcacc tacgacaagc ttctgcagct gagcgagaag ctcgacatcc
     6001 gcatcgccga cctgttcacg ggagccgacg cgccttcgga ccccggggtg accgggcgcc
     6061 gcagcatcgg acggatcgac gaggcggtgc gggtcgaaac gcccaattac gattattatt
     6121 atctctgcac cgatctcagg cggaagcgga tggtgccgat cctcacccgc atccgcgcca
     6181 agtcgctgag cgagttcggc gacctcatct cccatcccgg cgaggaatat atccacgtgc
     6241 tggagggtgc gatcgtcgtc cacaccgaat tctacgatcc ggtgacgctg aagacgggcg
     6301 aggcgatcta catcgattcg aacatgggcc acgcttatgt cgcggccgag gaatgcgagg
     6361 aggctttggt gctcggcgtc tgctcgagcg acgaccatgc gctgatgagc tcgctcatgc
     6421 agatgcacga gccgttgcat gcaggccgct ccacacccga cgcgctcggc acgaaggtgc
     6481 ggtcgctgcg caggcctgcc gcctagccgg ccgccgcgag cgactcggcg acacgcgcga
     6541 gggcgagctc gaccggctcg gcctcgccca tcaaagcgaa ggtgccgttg agcgcgaacg
     6601 tgcaatggcc gtggacggtc gcgatcagcg agcgggcgag gcggggcgcg ccggcgcggg
     6661 cggcctccgg cagcgcggcg gcgatttcct ctgtgacgat gcgggtgagc tcgccgcgct
     6721 cggcgtcata ttcttccggc atggcggcgc cgggtggcag gcggtggtcg taaatcgcca
     6781 tccacaggtt cggattggcg cgggcgaagc tgaaatagcc ctcgaccagc gcccgtatcc
     6841 ggtcgccttc cctcccctcc agcccggcgc ggaggtgatc ggcccataag gagaaggtgc
     6901 gcgtgttgat cgccaagatc agccggtcgt gggtgccgaa cacattgtag agggtgccga
     6961 tcgaatagcc gatccgcttg gcgacctcgc gcgcggagaa gcgcgagaag cccacctcgg
     7021 ctagctgcct gtggccttcg aggaggatca tccgctcgag ctcg
//
LOCUS       DASVRK010000053         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_257997, whole genome shotgun sequence.
ACCESSION   DASVRK010000053 DASVRK010000000
VERSION     DASVRK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257997"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF589_01955"
     CDS             <1..225
                     /locus_tag="VF589_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8256372.1"
                     /translation="NILASRPPSVGRDGLAAAMAAFADAADPDGKTAERFEILYLIGW
                     APSPDQPQPARRGSATASMAAALAPPSGED"
     gene            complement(222..1388)
                     /locus_tag="VF589_01960"
     CDS             complement(222..1388)
                     /locus_tag="VF589_01960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX8256373.1"
                     /translation="MSEVQYGWLLKAAKPIALLFLFAAVTAAFVNARHPIEMDYVSYW
                     AAAVLALGGDAAAAYDVAAHKAVELTEVPLNGSMPFPYPPAFLLLLLPFGLLPFSLSA
                     AVWTGTTCAAYLAAARRLLPGSGWIAAAYPAVLVNAVIGQNGFLTAAIFIAGLLCLPR
                     RPFAAGLVLGCLILKPQLGLLLPLAFIAGRDWKAFAGAAVSTLGLSLLGLIAFGPASY
                     AAWIGQMPLYTAIVSDGLVGWYKMASFYASLRLAGAPAEAAWAVHMLAAALAAAGVWT
                     TWRSDCSLAAKGALLAAATTLVSPYLYLYDTLLLIVPIFHLLRAGADRRLIALLWALP
                     MFAVLQNWVWNDTVNLTPFASLAILLLVWRQARLEVAVPESAGAAAPAPAGLPA"
BASE COUNT          279 a          529 c          529 g          170 t
ORIGIN      
        1 aacatcctcg cctcgcgccc gccttcggtc ggccgcgacg gattggccgc ggcgatggcc
       61 gccttcgccg acgcggcgga cccggacggc aagaccgccg aacgattcga gatcctctat
      121 ctgatcggct gggccccctc ccccgaccag ccccagccgg cgcggcgcgg cagcgcaacc
      181 gcctcgatgg cggcggcgtt ggcgccgccc tccggcgaag actaagcggg aagaccggcc
      241 ggagcgggcg ccgcggctcc agcgctttcg ggcacggcca cctcgagcct ggcctggcgc
      301 cagaccagca gcaggatcgc gagcgaggcg aacggcgtca ggttgacggt atcgttccac
      361 acccaattct gaagaaccgc gaacatcggc agcgcccaaa gcagcgcgat cagccgccgg
      421 tcggcgccgg cccgcaggag atggaagatc ggcacgatca gcagcaaagt atcgtagaga
      481 tagagatagg ggctgacgag cgtggtcgcc gcggcgagca atgcgccctt ggccgcgagg
      541 ctgcaatcgc tgcgccaggt cgtccacacc cccgccgccg ccagcgcggc cgccagcatg
      601 tgcaccgccc aggccgcctc cgccggcgcg ccggcgagcc gcagcgaggc gtagaagctc
      661 gccatcttgt accagccgac cagcccgtcg gagacgatcg ccgtgtaaag cggcatctgt
      721 ccgatccagg cggcatagga ggcgggcccg aaggcgatga ggccgagcag cgacaatccg
      781 agcgtcgaca ccgcggctcc cgcaaacgcc ttccagtcgc ggccggcgat gaaggcgagc
      841 ggcagcagca gcccgagctg cggcttcagg atcaggcagc cgagcaccaa cccggcggcg
      901 aacgggcggc gcggcaggca caataagccg gcgatgaaga tcgcggcggt gaggaagccg
      961 ttctggccga tgaccgcgtt gaccagcacc gccggatagg ccgccgcgat ccagcctgag
     1021 cccggtagca gccggcgcgc cgcggcgaga taggcggcgc aggtggtccc ggtccacacc
     1081 gccgccgaca ggctgaacgg caacagcccg aagggaagca ggagcagcag gaaggccggc
     1141 ggatacggga acggcatcga tccgttgagc ggcacctcgg tcagctcgac cgccttgtgc
     1201 gcggcgacgt cataggccgc ggcggcatcg ccgcccagcg ccagcaccgc agccgcccaa
     1261 tagctgacat agtccatctc gatcggatga cgcgcgttga cgaacgcggc ggtgacggcg
     1321 gcgaacagga acaggagggc gatcggcttc gcggccttta ggagccatcc atattgaacc
     1381 tctgacatga tcgcggcgcg gcctccgctg gcgacgataa ggacggttgg ttaacgaagt
     1441 ctcgcggccg gctcaggctc ggctgccgcg ccggccccga gcaatgcgtc gagcagcccg
     1501 atcagcg
//
LOCUS       DASVRK010000054         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_568845, whole genome shotgun sequence.
ACCESSION   DASVRK010000054 DASVRK010000000
VERSION     DASVRK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568845"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VF589_01965"
     CDS             complement(<1..886)
                     /locus_tag="VF589_01965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX8256374.1"
                     /translation="MKPGKEILFLALGGSGEIGMNVNLYGCDGKWVMVDLGMTFGDPN
                     YPGIELVLPDLAFIEERRKDLLGIVLTHGHEDHIGAIPYLAADLGVPLYATPFTAGLI
                     AGKLDEEGLTREVKLNRVDIGGTFQLGPFGFRFVPLAHSIPEGNALLIDTPYGKIFHT
                     GDWKLDEFPQIGDPADAAELIKVGDEGILALVCDSTNVFQEEASGSEREVRAGLDEVV
                     GAAAGRVLVTTFASNAARLDTLGKVAQDTGRQVCVAGRSLDRILRVARSVGYLLDFPD
                     TVDFDTAMDLPRRELMIIA"
     gene            complement(1016..>1767)
                     /locus_tag="VF589_01970"
     CDS             complement(1016..>1767)
                     /locus_tag="VF589_01970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX8256375.1"
                     /translation="VFALVEDGVIRARWRIATDPRRTADQYAVWLHQLIQLEGLPRDI
                     VSAVIIGTVVPRALHNLEVLAQKYFGVEPLVAGRAPADWGIELDVAEPQTVGADRALN
                     AIAAHAIHPGDLIVIDFGTATTFDMVDYSGAYKGGIIAPGINLSLDALVTAAARLPRI
                     AIEAPSGNLSVIGRTTEDQMQIGIYWGYVAMIEGLVERMKREIGHPVEVVATGGLATL
                     FDRHTAIFDAIEPDLTIQGLSLLYKSSLSAA"
BASE COUNT          289 a          517 c          667 g          294 t
ORIGIN      
        1 tggcgatgat catcaactcg cggcggggca ggtccatggc ggtgtcgaaa tcgaccgtgt
       61 cggggaagtc caggagatag ccgacgctgc gggcgacgcg caggatgcgg tcgagcgagc
      121 ggccggcgac gcagacctgg cggccggtgt cttgggcgac cttgccgagc gtgtcgaggc
      181 gggcggcgtt ggaggcgaag gtggtgacga gcaccctgcc cgcggccgcg ccgaccacct
      241 cgtcgaggcc ggcgcggacc tcgcgctcgg agcccgacgc ttcctcctgg aagacgttgg
      301 tcgaatcgca gaccaaagcg aggatgccct cgtcgccgac cttgatcagc tcggcggcgt
      361 cggcggggtc gccgatctgc gggaattcgt cgagcttcca gtcgccggtg tggaagattt
      421 tgccgtaggg cgtgtcgatg aggagcgcgt tgccttccgg gatcgaatgg gcgagcggca
      481 cgaagcggaa gccgaacggg ccgagctgga aggtgccgcc gatgtcgacg cggttgagct
      541 tcacctcgcg ggtcagcccc tcctcgtcga gcttgccggc aatgaggccg gcggtgaagg
      601 gcgtggcgta gagcggcacg ccgagatcgg cggcgagata ggggatggcg ccgatatggt
      661 cctcatggcc gtgggtgagg acgatgccga gcaggtcctt gcgccgctcc tcgatgaagg
      721 cgaggtcggg aaggacgagc tcgatgccgg gataattggg atcgccgaag gtcatgccga
      781 gatcgaccat cacccatttg ccgtcgcagc cgtagaggtt gacgttcatg ccgatctcgc
      841 cggagccgcc gagcgcgagg aacaggattt ctttgccggg tttcatttat cgggggatct
      901 ttctggttcg tcatccccgc gaacgcggga agcgcgcttc ctttccatcg ccgtcgggcg
      961 cgggagaagg aagggagaag cgggattccc gctttcgcgg gaatgtcggg gaggttcaag
     1021 cggcgcttag gctgcttttg tagagcagcg acagcccctg gatggtgaga tcgggctcga
     1081 tcgcgtcgaa aatggccgtg tgccggtcga acaaagtggc gaggccgccg gtggcgacga
     1141 cttcgaccgg gtggccgatc tcgcgcttca tgcgctcgac cagcccctcg atcatcgcga
     1201 catagcccca gtaaatcccg atctgcatct ggtcctcggt ggtgcggcca atgacggaga
     1261 ggttgccgga cggcgcctcg atggcgatgc gcggcagccg cgcggcggcg gtgacgagcg
     1321 cgtccagcga caggttgatg ccgggcgcga ttatgccgcc tttataggcg cccgaataat
     1381 cgaccatgtc gaaggtggtg gcggtgccga aatcgatgac gatgaggtcg cccggatgga
     1441 tggcgtgggc ggcgatggcg ttgagcgcgc ggtcggcgcc gaccgtctgc ggctcggcga
     1501 cgtcgagctc gatcccccag tcggcgggcg cgcggccggc gacgagcggc tcgaccccga
     1561 aatatttctg cgcgagcact tcgagattgt gcagcgcgcg cggcacgacg gtgccgatga
     1621 tgacggccga cacgatgtcg cgcggcagcc cttcgagctg gatgagctgg tggagccaga
     1681 ccgcatattg atcggcggtg cggcgcgggt cggtggcgat ccgccagcgc gcgcggatca
     1741 ccccgtcttc gaccagcgcg aagacga
//
LOCUS       DASVRK010000055         8348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_258472, whole genome shotgun sequence.
ACCESSION   DASVRK010000055 DASVRK010000000
VERSION     DASVRK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258472"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="VF589_01975"
     CDS             <1..179
                     /locus_tag="VF589_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX8256376.1"
                     /translation="LIAKIDSALRRIDTGEYGFCEVTGEPISLARLEARPIATMTVEA
                     QERHERQERVSRDD"
     gene            286..660
                     /locus_tag="VF589_01980"
     CDS             286..660
                     /locus_tag="VF589_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8256377.1"
                     /translation="MDEVIETKIEKSGVAAARDSRRTSARDSMLLSATIRRAQDPDAE
                     LKPVRVRNLSAIGLMADYSGVAIPGDAVVVTVRGIGKVSGKVAWLKRGRIGITFDIEV
                     DPLMARKPVVPPSRQGVKQRPL"
     gene            complement(734..892)
                     /locus_tag="VF589_01985"
     CDS             complement(734..892)
                     /locus_tag="VF589_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX8256378.1"
                     /translation="MQQAHMSALEQKHAGLDARIAEENQRPHPDDVLLSRLKKEKLRI
                     KEAIAGIA"
     gene            1051..1260
                     /locus_tag="VF589_01990"
     CDS             1051..1260
                     /locus_tag="VF589_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX8256379.1"
                     /translation="MPIDASNAGLAQRLARLRMEHRDLDAAIDALRSLAAPDQLQIAR
                     LKRQKLRMRDEIAAIEDQMVPDIIA"
     gene            1379..1813
                     /locus_tag="VF589_01995"
     CDS             1379..1813
                     /locus_tag="VF589_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX8256380.1"
                     /translation="MTPKLIDSLYVEAMLLADEARAYFDRHGRADRMALDPLVRVGFS
                     CESLKVTTRLMHVIAWLLTQRAVEAGELPRAAVRRPVRRLGHAAESDTELLGQLPEAA
                     LQLVQASRDLYARVQRLDEGSGTLEPQASPARSLLSRLERSF"
     gene            1852..2997
                     /locus_tag="VF589_02000"
     CDS             1852..2997
                     /locus_tag="VF589_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8256381.1"
                     /translation="MHAYEFLYGPKLIAGAGAAARLGELLPAGRCLFVTDAGLRALGL
                     ADAPLAAFTGRGVEPVIFDSVEADPSRATLIEAVTLGLEQGCASVVGFGGGSPMDVAK
                     LAAYLIGSGDDLDTLWGVGKAAGAGLPLALVPTTAGTGSEATPVSVITVGETEKRGVS
                     SAALIADWAILDAELTVGLPRPVTAATGIDAMVHAIEAYTSAHRKNPLSDMLALEALR
                     LLAGNLLDACERPRDIEAREAMLLGAHLAGVAFANAPVAGVHALAYPLGGYFHVPHGL
                     SNALMLPHVLGHNMPAAMALYAELGVVVEPSLAAQGRQAQAQGLVNRLAELARAAGLP
                     ERLSAVGVAAGDLDLLAAEAMKQERLLVNNPCPITEADARRLYEAAL"
     gene            2994..3428
                     /locus_tag="VF589_02005"
     CDS             2994..3428
                     /locus_tag="VF589_02005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8256382.1"
                     /translation="MSREPMRGRAAYRVWREIGTRWADNDAYGHVNNAVYYGWFDTAV
                     NAWLIEAGLLDVAAGDPIGLVVETGCRYAHPLTYPEPVDIGLAVDEVGRSSVRYRLGA
                     FAKGRDAPAAEAIFVHVYVGRESRRPVELPEAWRNAFDEIRG"
     gene            complement(3553..4515)
                     /locus_tag="VF589_02010"
     CDS             complement(3553..4515)
                     /locus_tag="VF589_02010"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX8256383.1"
                     /translation="MPLPAPLDRLRLPVIGAPLFIVSNPDLVIAQCKAGIVGSFPALN
                     ARPQPLLDEWLDRITEELAAWDRDHPDRPAAPFAVNQIVHRSNDRLEADMATCAKWKV
                     PIVITSLGALVEVNQAVHGWGGITLHDIINDRFARKAVEKGADGLIAVATGAGGHAGT
                     LSPFALVQEIRDWFEGPLILSGAIANGHAVLAALAMGADLAYIGSPFIATEEANAAAD
                     YKHGIVAGRAADIVYSSLFTGVHGNYLRQSIAASGLDPDKLPEGDLKTMDFGGGQSAK
                     AWRDIWGSGQGIGAVTKVQPAADYVATLAAEYEAALRELTTKTG"
     gene            complement(4729..6111)
                     /locus_tag="VF589_02015"
     CDS             complement(4729..6111)
                     /locus_tag="VF589_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX8256384.1"
                     /translation="MSHRFDPTSLREYDVRGIVGETLREADAHALGRSFATLVRRGGG
                     TRAAVGRDGRESSPVLEAALIRGLNESGVDVVRVGLGPTPMLYFAEAELEVDGGIMIT
                     GSHNPANYNGFKMVMFGSAFFGQDIQKLGRMAEAGDWDEGSGTATEADVMDLYIDRLL
                     KNFDGAPFRIGWDAGNGAAGPALERLTARLPGEHHLLYTDIDGSFPNHHPDPTEEKNL
                     EDLKRLVSDKGLDFGIAFDGDGDRIGAVDGSGRVVWGDQLLSILAEPVLNDVPGATII
                     ADVKASQALFDRVAELGGKPLMWKTGHSLIKSKMKETGAPLAGEMSGHIFFAHDYYGF
                     DDALYAAVRLIRAVSQLGGSLTALRSAMPAMVNTPEMRFQVDESRKFPVIDEVLARLE
                     TDGAKVDRTDGARVNTDDGWWLLRASNTQDVLVARAEAKDESALARLMGQIDEQLALS
                     GLERGPQAGH"
     gene            complement(6141..6557)
                     /locus_tag="VF589_02020"
     CDS             complement(6141..6557)
                     /locus_tag="VF589_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8256385.1"
                     /translation="MPGVVILALAAGLGWAWWSGRLKGFTYEDGVAAALFLLGLRLLT
                     TGRLLPGAALMAGAILWAAYRRGKSAPPTMPVEDARKLLGVGDGASLAEIRDAHRRLI
                     ARVHPDAGGSAELANRVNVARDTLVAEMNRRTPRAS"
     gene            complement(6562..7371)
                     /locus_tag="VF589_02025"
     CDS             complement(6562..7371)
                     /locus_tag="VF589_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEX8256386.1"
                     /translation="MAGAAPHFIVFANEKGGTGKSTTAVHSAVALAAAGRRVAAIDLD
                     TRQRTLGRYLDNRAASIRRLATELPMPLYETFDPDKNDDLDAQLDRLAADVEVVVVDT
                     PGRDDPYARQAMLRADTLVTPINDSFVDLDLIGEVDAETYKVRRPSFYAELVWKSRTQ
                     RAKTTGASVDWVLLRNRMQHIEARNMRRVGAALDELSRRVGFRVIPGLGERVIYRELF
                     PKGLTLLDLGHLGEVGLSHIAARQELREMIAGFGIPEEGKKAPQPRAALAG"
     gene            7468..8319
                     /gene="panC"
                     /locus_tag="VF589_02030"
     CDS             7468..8319
                     /gene="panC"
                     /locus_tag="VF589_02030"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX8256387.1"
                     /translation="MQIVREITALRRAVAELRADGGTIALVPTMGALHAGHMALVAEA
                     RRRARHVVASIFVNPKQFGANEDLASYPRREATDGAMLDAEGVALLWAPEAADMYGDG
                     YATTVSVAGVSDGLDGAARPGHFDGVATVVAKLFNQVRPDLALFGEKDYQQLAVVRRM
                     SADLDLDVEIVAVPTQRDADGLALSSRNIYLSDEERRAARALPRALGEAARTIGAGGA
                     AADALATARERLAAAGFDPVDYVELRDAATLEPLAALDRAARILAAGRIGRTRLVDNV
                     AVEPQPE"
BASE COUNT         1239 a         2699 c         3056 g         1354 t
ORIGIN      
        1 agctgatcgc caagatcgat tcggccctgc ggcgcatcga caccggcgaa tatggctttt
       61 gcgaagtcac cggcgagccg atctcgctgg cccggctcga ggcccggccg atcgccacca
      121 tgacggtcga ggcgcaggag cgccacgaac gccaggagcg ggtttcccgc gacgactgag
      181 gcgccgcccc cgaccgcttt gcgacggggg aggaaagccg gcgttaactc gatcgcgacc
      241 tttctgcggt agctctgcct ccgggccggc gggccgatcc ggagggtgga tgaagtgatt
      301 gagaccaaga tcgagaagag cggagtcgct gcggcgcggg acagccggcg gacgtcggcc
      361 cgcgacagca tgttgctgtc ggcgacgatc cgccgggccc aggaccccga cgccgaactc
      421 aagccggtgc gggtgcgcaa cctgtccgcg atcgggctga tggcggatta tagcggggtt
      481 gccattcccg gcgacgcggt ggtcgtgacg gtgcgcggca tcggcaaggt ttcgggcaag
      541 gtcgcgtggc tgaagcgggg ccgcatcggc ataaccttcg acatcgaggt cgatccgctg
      601 atggcgcgca aaccggtcgt gccgccgtcg cggcaagggg tgaagcagcg cccgctctaa
      661 tgtagcgtcg ctgtaaggcg gctcccgctt aagggaagcg ggagcgcgta aggaagcggg
      721 agccttgggc cgctcaggcg atgccggcga tcgcttcctt gatcctcaat ttttctttct
      781 tgaggcggga aaggagcacg tcgtccggat gggggcgctg attctcttcg gcgattcggg
      841 catcgagacc agcatgcttt tgttcgagtg cggacatatg cgcctgttgc attcccttct
      901 cctctgtgcg gttgatgctg acggttagaa cagcctattc ggctcctgtc tccctcggtc
      961 ggacgggtgc ccatctatgt acgacgaccc gttgatcgat tatctatatc ccagaatttc
     1021 gtcgttgagt agcgggggcc gcagggcatg atgccgatcg acgcaagcaa tgccggcctg
     1081 gcgcaaaggc tggcgcggct gaggatggag catcgcgacc tcgacgctgc gatcgatgcg
     1141 ctccgcagcc tggccgcgcc cgaccagctg cagatcgcca ggctgaagcg ccagaagctc
     1201 aggatgcgcg acgagatcgc cgccatcgag gaccagatgg tgcccgacat catcgcctga
     1261 ggccgcccga tcgctcccgc agtgggacgg ggctccggat tcaccttctt tcgcaattcc
     1321 cgcttgctcc cggctatggc aatgtagctt tatggccggt gaggatgcgg gggcgcggat
     1381 gacgccgaaa ctgatcgatt cgctctacgt ggaggcgatg cttctggcgg acgaggcgcg
     1441 cgcctatttc gaccgccacg gccgtgccga ccggatggcg ctcgatcctt tggtccgcgt
     1501 cggcttttcc tgcgaatcgc tcaaggtgac gacgcggctg atgcacgtca tcgcctggct
     1561 gctcacccag cgcgccgtcg aggcgggcga attgccgcgc gcggccgtcc ggaggccggt
     1621 gcggcggctc ggccacgccg ccgagagcga cacggagctg ctcggccagc tccccgaggc
     1681 ggcgctgcaa ttggtccagg cgagccgcga tctctatgcg cgggtgcagc ggctcgacga
     1741 aggctccggc acgttggagc cgcaggccag tccggcccgc agcctgctga gccggctcga
     1801 gcgctccttc tgaatttgcg ctgcgggcgg cggcgcggct agaccgcggc catgcacgct
     1861 tacgaatttc tctacggccc gaagctgatc gcgggcgccg gcgcggcggc gaggctcggc
     1921 gagctgctgc cggccgggcg ctgcctgttc gtcacggatg cggggctgcg cgcgctcggc
     1981 cttgccgatg cgccgctcgc ggcttttacc ggccgcggcg tcgagccggt catcttcgac
     2041 tcggtcgagg cggatccctc gcgggcaacc ctgatcgagg cggtcacctt aggtctggag
     2101 cagggctgcg cctccgtcgt cggcttcggc ggcggcagcc cgatggatgt ggccaagctc
     2161 gccgcctatc tgatcggcag cggcgacgat ctcgacacgc tctggggggt cggcaaggcg
     2221 gccggtgcgg ggctgccgct ggcgctggtg ccgaccacgg cggggaccgg ctcggaagcc
     2281 accccggtca gcgtgatcac ggtcggcgag accgaaaaga ggggcgtcag cagcgccgct
     2341 ttgatcgccg actgggcgat cctggacgca gagctgacgg ttggcctgcc gcggccggtc
     2401 acggccgcga ccggaatcga cgcgatggtg cacgcgatcg aggcctatac gtcggcgcac
     2461 aggaagaacc cgctgtcgga catgctggcg ctcgaagcgc ttcggctgct ggccgggaac
     2521 ctgctcgacg cctgcgagcg gccccgggac atcgaagcgc gggaggcgat gctgctcggc
     2581 gcgcaccttg ccggggtggc gttcgccaac gcgccggtgg cgggcgtgca tgcgctggct
     2641 tatccgctcg gcggctattt ccatgtcccg cacgggcttt ccaacgcctt gatgctgccg
     2701 cacgtgctgg gccacaatat gccggccgcg atggccctgt acgcggagct cggagtcgtg
     2761 gtggagccaa gcctggcggc ccaggggcgg caggcgcagg cgcaggggct ggtcaatcgg
     2821 ttggcggagc tcgcccgcgc ggccgggctg ccggagcgat tgtcggcggt cggcgtggcg
     2881 gccggcgacc tcgatttgct ggcggccgag gcgatgaagc aggagcggct gctggtgaac
     2941 aatccatgcc cgatcacgga ggccgatgcc cggcggcttt acgaggcggc gctgtgagcc
     3001 gggagccgat gcgcgggcga gcggcctacc gcgtgtggcg cgagatcggc acgcgctggg
     3061 ccgacaacga cgcctacggc cacgtcaaca acgccgttta ttatggctgg ttcgacacgg
     3121 cggtgaatgc ctggctgatc gaggcggggc tgctcgacgt ggcggccggc gacccgatcg
     3181 gcctggtggt ggagaccggc tgccgctacg cgcatccgct tacctatccg gagccggtcg
     3241 atatcggcct cgccgtggac gaggtcggcc gatcgagcgt ccgctaccgc ctaggcgctt
     3301 tcgccaaggg gcgggacgca ccggcggcgg aggcgatctt cgtccatgtc tatgtcgggc
     3361 gggagagccg gcggccggtc gagctgccgg aagcatggcg gaacgcgttc gacgaaatca
     3421 gaggttgagc tcctccccgg aacggggagg gggaccgccg ccgcaggcgg tggtggaggg
     3481 gctgcgcgaa gcaccgaccc ccctccacca tgctgcgcat ggtccccctc cccgagcaag
     3541 ctcggggagg aactagcccg tcttcgtcgt tagctcccgc agggccgcct catattccgc
     3601 ggcaagcgtg gcgacgtaat ccgctgccgg ctggaccttg gtcaccgcgc cgatgccttg
     3661 gcccgagccc caaatgtccc gccaggcctt ggcgctttgg ccgccgccga agtccatcgt
     3721 cttgaggtcg ccttcgggca gcttgtcggg gtcaaggccc gaagccgcga tcgactggcg
     3781 caggtaattg ccgtggacgc cggtgaacag gctcgaatag acgatgtcgg cggcgcggcc
     3841 ggcgacgatg ccgtgcttgt aatcggcggc ggcattggcc tcttcggtgg cgatgaacgg
     3901 cgagccgata taggcaaggt ccgcgcccat cgccaaagcg gcgaggacgg cgtgcccgtt
     3961 ggcgatcgcc cccgacagga tcagcggccc ttcgaaccag tcgcgtatct cctggacgag
     4021 cgcgaagggc gacaaggtgc cggcatggcc gcccgctccc gtggccaccg cgatgaggcc
     4081 gtcggcgccc ttctcgaccg ccttgcgcgc gaagcggtcg ttgatgatgt cgtggagggt
     4141 gatgccgccc cagccgtgga ccgcctggtt gacctcgacc agcgcgccca gcgaggtgat
     4201 gacgatcggc accttccatt tggcgcaggt cgccatgtcg gcctcgagcc ggtcgttgga
     4261 gcggtggacg atctggttga ccgcgaacgg ggcggcgggg cgatcgggat ggtcgcggtc
     4321 ccaggcggcg agctcttcgg tgatccggtc cagccattcg tcgagcagcg gctgcgggcg
     4381 ggcgttcagg gcagggaagg agccgacgat gccggccttg cactgggcga tcaccaggtc
     4441 cgggttggag acgatgaaca gcggcgcgcc gatgacgggc aggcgcaggc ggtcgagcgg
     4501 ggcggggagc ggcatcggct ttcccttaac gtaaaggagg ggggcgtcaa tgcgggagcg
     4561 taacggtgtg tcggatcacc taggcgaact ggcctgaggt gcagccgtgg cgacacccgt
     4621 cattccagcg aaagctggaa tctctctacc ttggtgagcc gaagaaaagg gagatcccag
     4681 ctttcgctgg gatgacgggg tggctgctaa cgggctccct gaccgggctc agtgtcccgc
     4741 ctgcggccct cgctccagcc ccgaaagcgc gagctgttcg tcgatctgcc ccatcaggcg
     4801 cgcgagcgcc gactcgtcct tggcctcggc gcgggcgacg aggacgtcct gggtgttgga
     4861 ggcgcgcagc agccaccagc cgtcgtccgt gttgacccgc gcgccgtcgg tgcggtcgac
     4921 cttggcgccg tccgtttcga gccgggcgag aacttcgtcg atgaccggga atttgcggct
     4981 ctcgtcgacc tggaagcgca tttcgggagt gttgaccatc gccggcatcg ccgaacggag
     5041 ggccgtgagc gagccgccga gctgcgagac ggcgcggatc aggcggacgg cggcgtagag
     5101 cgcgtcgtcg aagccgtaat aatcgtgggc gaagaagata tggccgctca tctcgccggc
     5161 gagcggggcg ccggtctcct tcatcttcga cttgatcagg ctgtggccgg tcttccacat
     5221 cagcggcttg ccgccgagct cggcgacgcg gtcgaacaag gcctggctcg ccttgacgtc
     5281 ggcgatgatg gtggcgccgg ggacgtcgtt cagcaccggc tcggccagga tcgagagcaa
     5341 ttgatcgccc caaacgaccc ggccggagcc gtcgaccgcg ccgatccggt cgccgtcgcc
     5401 gtcaaaggcg atgccgaaat cgaggccttt gtcgctgacc agccgcttca gatcctcgag
     5461 gttcttctcc tcggtcggat cgggatggtg gttggggaag ctgccgtcga tgtcggtata
     5521 gagcaggtga tgctcgccgg gcaggcgcgc ggtcagccgc tccagcgccg ggccggcggc
     5581 gccgttgccg gcgtcccagc cgatgcggaa cggggcgccg tcgaaattct tgagcaggcg
     5641 gtcgatgtaa aggtccatca cgtcggcttc ggtggcggtg ccggagccct cgtcccaatc
     5701 gcccgcttcg gccatgcggc cgagtttctg gatgtcctgg ccgaagaaag cgctgccgaa
     5761 catcaccatc ttgaagccgt tgtaattggc cggattgtgg ctgccggtga tcatgatgcc
     5821 gccgtcgacc tcgagctcgg cttcggcgaa atagagcatc ggcgtcgggc cgagcccgac
     5881 ccgcaccacg tcgacgccac tctcgttgag gccccggatc aaagccgctt ccagcaccgg
     5941 ggagctctcg cgcccgtcgc ggccgacggc ggcgcgggtg ccgccgccgc ggcggaccaa
     6001 ggtggcgaag ctgcggccga gcgcatgcgc atccgcctcg cgcaaggttt cgccgacgat
     6061 cccgcggacg tcatattcgc gcagcgaggt cgggtcgaaa cgatgactca tgggatgctc
     6121 cattgatgcg gggattagga tcaggacgcg cggggcgtcc tgcggttcat ttccgcgacc
     6181 aaagtgtcgc gcgcgacgtt gacgcggttg gcgagctcgg ccgagccgcc ggcatcgggg
     6241 tggacgcggg cgatcagccg ccggtgggcg tcgcggattt ccgccaggct ggcgccgtcg
     6301 ccgacgccga gcagcttgcg cgcatcctcg accggcattg tcggcggcgc cgacttgccg
     6361 cgccgatagg cggcccacag gatcgcgccg gccatcaagg cggcgccggg cagcaggcgg
     6421 ccggtggtca gcagccgaag gccgagcagg aacaaagccg ccgcgactcc atcttcataa
     6481 gtgaagccct tgaggcggcc gctccaccag gcccagccga gcccggcggc gagcgcaagg
     6541 atgacgacgc ccggcaccgg ctcagccggc gagggcggcg cgcggctgcg gcgccttctt
     6601 gccttcttcg ggaatgccga agccggcgat catctcgcgc agctcctggc gcgcggcgat
     6661 gtggctgagg ccgacttcgc cgagatggcc gaggtcgagc agggtcaggc ccttggggaa
     6721 cagctcgcgg tagatgacgc gctcgccgag cccggggatg acgcggaagc cgacgcggcg
     6781 ggaaagctcg tccagcgcgg cgccgacgcg gcgcatgttg cgcgcttcga tatgctgcat
     6841 gcggttcctg agcagcaccc agtcgacgct ggcgccggtg gtcttggcgc gctgggtgcg
     6901 gctcttccag accagctccg cgtaaaagct cggccggcgg accttgtagg tctcggcgtc
     6961 gacctcgccg atcaggtcga gatcgacgaa gctgtcgttg atcggcgtca ccagcgtgtc
     7021 ggcgcggagc atggcctggc gggcataagg atcgtcgcgg ccgggcgtgt cgaccaccac
     7081 cacctcgaca tcggcggcaa gccggtcgag ctgggcatcg agatcgtcat tcttgtccgg
     7141 gtcgaaggtc tcgtagagcg gcatcggcag ctcggttgcc agccgccgga tcgaggcggc
     7201 acgattgtcg agatagcggc cgagggtgcg ctggcgcgtg tcgaggtcga tcgccgccac
     7261 ccgccgcccc gccgccgcca gcgcgaccgc gctatggacg gcggtcgtgg atttgccggt
     7321 gccgcccttt tcgttggcga atacgatgaa atgcggcgct gccccagcca tcctgccccg
     7381 tctgcccctc ttgatcttgg ttcggcgcgc ccgcaaagag cgcgctcctt ccttcaagtt
     7441 ccagcctgta tcggagccca tgcggcggtg caaatagtcc gtgaaatcac tgcgcttagg
     7501 cgtgccgtgg cggagttgag agcggacggc ggcacgatcg cgctggtgcc gaccatgggc
     7561 gcgctccacg ccggccacat ggcgctggtg gcggaggcgc ggcggcgcgc ccggcatgtg
     7621 gtcgcctcga tcttcgtcaa tcccaagcaa ttcggcgcca atgaggatct cgccagctat
     7681 ccgcgccgcg aggcgactga tggggcgatg ctcgatgccg agggcgtggc cctgctgtgg
     7741 gcgcctgagg cggcggacat gtatggcgat ggctatgcga cgaccgtgag cgtggccggg
     7801 gtcagcgacg gcctcgacgg ggcggcgcgg cccggccatt tcgacggagt cgcgacggtg
     7861 gtggcgaagc tgttcaacca ggtgcggccc gacctcgcgc tattcgggga aaaggattat
     7921 cagcagcttg ccgtcgtccg aaggatgagc gcggacctcg acctcgacgt cgagatcgtc
     7981 gccgtgccga cgcagcgcga cgccgacggc ctcgctctgt cgtcgcgcaa catctatttg
     8041 tcggacgagg aacggcgggc ggcgcgggcg ctgccgcgcg cgctcggcga ggcggcgcgg
     8101 acgatcggcg ccggcggcgc ggcggccgac gcgctggcga cggcgcgcga gcggctggcg
     8161 gcggcgggct tcgatccggt cgactatgtc gagctgcgcg acgccgcgac gctggagccg
     8221 ctggcggcgc tcgaccgcgc ggcgcgtatc ctcgccgccg ggcggatcgg ccggacccgc
     8281 ctcgtcgaca atgtggccgt cgagccgcag cccgaatgag gtcactttcg cgttaaccat
     8341 ttctttgc
//
LOCUS       DASVRK010000056         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_196242, whole genome shotgun sequence.
ACCESSION   DASVRK010000056 DASVRK010000000
VERSION     DASVRK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196242"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VF589_02035"
     CDS             <1..509
                     /locus_tag="VF589_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX8256388.1"
                     /translation="TYSRPFWEYRLGSVGMIIGAVIIALTAFALSFILSSVERFVVSF
                     VPGADTLFLNLFRLAPAAMLFGSLYLLFYTLTPKRYRTKCRKWPGPAFVTIWWLATTA
                     LLPVVLSSLGSYDLTYGSLAGVIIALFFFFIIGLGVVIGAELNAALAETPDEALKEPE
                     PNHSEATI"
     gene            506..1309
                     /gene="fabI"
                     /locus_tag="VF589_02040"
     CDS             506..1309
                     /gene="fabI"
                     /locus_tag="VF589_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX8256389.1"
                     /translation="MSGLMAGKRGLIMGLANDRSLAWGIARALGEHGAELAFSYQGDA
                     LRKRVAPLAGQVGSDFLIDCDVSDMAAIDRAFAEIEARWGRLDFLVHAIGFSDKNELR
                     GKFVDTSLDNFLMSMNISVYSFVAVTRAARRLMPDGGSILTLSYYGAEKVIPHYNVMG
                     VAKAGLEASVKYLAMDLGPEKIRVNAVSAGPIKTLAASGIGDFRYIMKWNEYNSPLRR
                     NVTIEDVGGAALYLLSDLASGVTGEIHHVDAGYNVVGMKAEDAPDIATV"
     gene            1320..1682
                     /locus_tag="VF589_02045"
     CDS             1320..1682
                     /locus_tag="VF589_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256390.1"
                     /translation="MPQISIEYSANIAATFDARRFTGEAHKLLVAMVETELASCKTRL
                     IELRDVVIGDGSDANAMVHVDLRILSGRTPAQKRQLGEAVMAALAAAVARPEGLDLQL
                     TVEVRDLDRDHYHKQRLR"
     gene            1679..2599
                     /locus_tag="VF589_02050"
     CDS             1679..2599
                     /locus_tag="VF589_02050"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX8256391.1"
                     /translation="MSVNGFGRLFRFTTWGESHGPQIGAVVDGCPPGLALSEGDIQPW
                     LDRRRPGLSRHTSPRAEPDRVRILSGVYEGQTTGTPISLVIDNVDARPKDYAGQPHRA
                     GHADSTYEAKYGLRDPRGGGRASARETAARVAAGAVARLVIPEVSIEARPVEIGGSGD
                     ESRWEALLDEARAAGSSLGAVIEGVARGVPAGWGAPVYAKLDAELAGAIMSINAVKGV
                     EIGAGFGVARRRGEDNAEPDFAGGIVGGIASGGPILLRAAFKPTSSVPALGVTGRHDP
                     CVGLRGAPVVEAMMALVLADHKLLHRAQCG"
     gene            2596..2874
                     /locus_tag="VF589_02055"
     CDS             2596..2874
                     /locus_tag="VF589_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX8256392.1"
                     /translation="MSGARRLLVSGLVQGVFYRNWAVAQARELGLAGWVRNLRSGNVE
                     LLAVGDEEALQSLVERCRRGPPAAVVEDVQVSAAEAGPLEGFEKRPTA"
     gene            complement(2969..3715)
                     /locus_tag="VF589_02060"
     CDS             complement(2969..3715)
                     /locus_tag="VF589_02060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8256393.1"
                     /translation="MTSREVTGLLPRADQAQASAWRRTFADWATLLAFGPINAPWLLR
                     SLHGGSKAGKRALLERLGLPQDALPNLGSWKADVGLLTLVADHILSQKPKLVVEFGAG
                     ASTLIIARALELAGGGTHIAFDQHADFVEATRDWLAEHGLAADLRAVPLVPAADGWPG
                     LWYDHGPLPDAIDLMLIDGPPWTIHPFTRGSAATLFGRIAPGGTILLDDGARPGERVI
                     ARRWRKDWPEFDFELVKSGTKGTLVGRRRG"
BASE COUNT          573 a         1251 c         1374 g          585 t
ORIGIN      
        1 tcacttattc gcggccgttc tgggagtacc ggctgggctc ggtggggatg atcatcggcg
       61 cggtcatcat cgcgctgacc gccttcgccc tctccttcat cctgtcgagc gtcgagcggt
      121 tcgtggtcag cttcgtgccc ggagccgaca cattgttcct gaacctgttc aggctggccc
      181 ccgccgcgat gctgttcgga tcgctctacc tgctcttcta cacgctgacg cccaagcgct
      241 atcgcaccaa gtgccgcaag tggcccggcc ccgccttcgt caccatctgg tggctggcga
      301 ccaccgcctt gctgccggtg gtgctgtcct cgctcggcag ctacgacctc acctacggca
      361 gcctggccgg ggtgatcatc gccttgttct tcttcttcat catcggcctc ggcgtcgtaa
      421 tcggggccga gctcaacgcc gcactagccg agacgccgga tgaagcccta aaggagccgg
      481 agccaaatca cagcgaggcg acaatatgag cggattgatg gcgggcaagc gcgggctgat
      541 catgggcctc gccaacgacc gctcgctcgc ctgggggatc gcccgcgcgc tcggcgagca
      601 tggcgccgag cttgccttca gctaccaggg cgatgcgctg aggaagcggg tggcgccgct
      661 ggccgggcag gtcggatcgg atttcctgat cgattgcgac gtttccgaca tggcggcgat
      721 cgaccgcgcc ttcgccgaga tcgaggcgcg ctggggccgg ctcgacttcc tcgtccacgc
      781 gatcggcttt tcggacaaga acgagcttcg cggcaagttc gtcgacacca gcctcgacaa
      841 tttcctcatg tcgatgaaca tctcggtcta cagcttcgtc gccgtcaccc gcgccgcgcg
      901 ccggctgatg cccgacggcg gcagcatcct cacgctcagc tattacggcg ccgagaaggt
      961 gatcccgcat tacaatgtga tgggcgtcgc caaggccggg ctcgaggcga gcgtcaaata
     1021 tctcgcgatg gacctcggcc ccgagaagat ccgcgtcaac gcggtctcgg ccggcccgat
     1081 caagacgctg gcggcgagcg gcatcggcga cttccgctac atcatgaagt ggaacgaata
     1141 taattcgccg ctgcgccgca acgtcacgat cgaggatgtc ggcggcgccg cgctctatct
     1201 gctgtcggat ctcgccagcg gggtcaccgg cgagatccac cacgtcgatg ccggctataa
     1261 cgtggtcggg atgaaggcgg aggacgcgcc ggacatcgcc accgtctgag gaggcgcgca
     1321 tgccgcagat cagcatcgaa tatagcgcca acatcgccgc cacctttgac gcccggcggt
     1381 tcacgggcga ggcccacaaa ctgctggtcg ccatggtcga gaccgagctg gcgagctgca
     1441 agacgcgcct gatcgagctt cgcgacgtgg tgatcggcga cggatcggac gccaatgcga
     1501 tggtccatgt cgacctgcgc atcctttcgg gacgaacacc ggcgcagaag cggcagctcg
     1561 gcgaggcggt gatggcggcg ctcgccgcgg cggtggcgag acctgagggg ctcgacctcc
     1621 agcttacggt cgaggtgcgc gacctcgacc gcgaccatta ccacaagcag cggctccgat
     1681 gagcgtcaac ggcttcggcc ggctcttccg cttcaccacc tggggcgaaa gccatgggcc
     1741 gcagatcggg gcggtggtgg acggctgccc gcccggactc gcgctgagcg agggcgacat
     1801 ccagccttgg ctcgaccggc ggcggccggg cctgtcgcgg cacaccagcc cgcgcgccga
     1861 gccggaccgg gtccgcatcc tctccggcgt ctatgaaggc cagaccacgg ggacgccgat
     1921 cagcctcgtc atcgacaatg tcgatgcgcg gcccaaggat tatgccgggc agccgcaccg
     1981 ggccggccat gccgactcca cctatgaggc gaaatacggg ctgcgcgacc cgcgcggcgg
     2041 cgggcgggcc tcggcgcgcg agacggcggc gcgagtcgcg gccggagcgg tggcgcggct
     2101 cgtcatcccc gaagtctcga tcgaagccag gccggtggag attggcggct ccggcgacga
     2161 gagccggtgg gaggccctgc tcgacgaggc gcgagcggcg gggagctcgc tcggcgccgt
     2221 gatcgagggc gtggcgcgcg gcgtgccggc cggctggggc gcgccggtct acgccaagct
     2281 cgacgccgag cttgccgggg cgatcatgtc gatcaacgcc gtcaaggggg tcgagatcgg
     2341 cgccggcttc ggcgtcgccc gtcggcgcgg ggaggataat gcggagccgg atttcgccgg
     2401 cggcattgtg ggcggcatcg ccagcggcgg gccgatattg ctgcgtgccg cgttcaagcc
     2461 gacctcgtcg gtgcccgcgc tcggcgtcac cggccgccac gacccttgcg tcggcctgcg
     2521 cggcgcgccg gtggtcgagg cgatgatggc gctggtgctc gccgaccaca agctgctcca
     2581 ccgggcgcaa tgcggatgag cggcgcgcga cggctcctgg tcagcggctt ggtgcagggc
     2641 gtattctacc gcaactgggc ggtggcgcag gcgcgcgagc tcgggctggc gggctgggtg
     2701 cggaacctcc ggagcgggaa tgtcgagctg cttgccgtgg gtgatgagga agctttgcag
     2761 agcctggtcg agcgttgccg gcggggaccg ccggccgcgg tggtggagga tgtgcaggtg
     2821 agcgccgcgg aagcggggcc gctggagggg ttcgagaagc ggccgacggc ttaggatgcc
     2881 ttgcttatgt tccccggcga aagccggggt ccaggaagga agggtctgga cctcggcctt
     2941 cgccggggaa ccattggcgt gcgcggcgct agcctctccg ccgccccacc aaagtcccct
     3001 tggtgccgct cttcaccagc tcgaaatcga attccggcca gtccttgcgc cagcggcggg
     3061 cgatgacgcg ttcgccgggg cgggcgccgt cgtcgaggag gatcgtgccg ccgggcgcga
     3121 tccggccgaa caaggtggcg gcgctgccgc gggtgaaggg gtggatcgtc cacggcggtc
     3181 cgtcgatcag catcaggtcg atggcgtcgg gcagcggccc gtgatcgtac cacaggccgg
     3241 gccagccgtc cgcggccggc accagcggaa ccgcgcgcag gtccgccgcc agcccgtgct
     3301 cagcgagcca gtcgcgcgtc gcctcgacga aatcggcatg ctggtcgaag gcgatgtgag
     3361 tgccgccgcc ggccagctcg agcgcgcggg cgatgatcag ggtggaggcg ccggcgccga
     3421 attcgacgac cagcttcggc ttctggctga ggatatggtc ggcgacgagg gtgagcagcc
     3481 cgacatcggc cttccagctg cccagattgg ggagcgcgtc ctggggcaag ccgagccgct
     3541 cgagcagagc gcgcttgccg gccttgctgc cgccgtggag gctgcgcagc aaccagggcg
     3601 cgttgatcgg gccgaacgcg agcaaggtcg cccagtcggc gaacgtgcgc cgccacgccg
     3661 aggcctgggc ctggtcggcg cggggcagca gcccggtgac ctcgcgcgag gtcatcaatc
     3721 ggcgaacgga tcgcgcacga ggatggtgtc ctcgcgctcg gggctggtcg agaccagggc
     3781 gac
//
LOCUS       DASVRK010000057        11382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_72228, whole genome shotgun sequence.
ACCESSION   DASVRK010000057 DASVRK010000000
VERSION     DASVRK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72228"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..425
                     /locus_tag="VF589_02065"
     CDS             123..425
                     /locus_tag="VF589_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8256394.1"
                     /translation="MAYDPGALNASLAAAVGSDPGLVGELRAAFVESAERQVDLMARA
                     RCDANWQLAASRLKSLGASFGAIGLIALADEALDGAPGDPVVLRKLRAAVDDFASM"
     gene            498..1688
                     /locus_tag="VF589_02070"
     CDS             498..1688
                     /locus_tag="VF589_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256395.1"
                     /translation="MAALICAYQESDEPGGALRATLPLAGRSVVERQARLAAAAGAER
                     IIILVERLPPDLAAAIDRLRSEGVSALVARGVAEAAASVQPADRLLFVADGLIAGQGH
                     LSRLVEAQGTAVLTVPDQSVDDRFERIDAHSRWAGLALLDGEMLRRTADMLEDWDLQS
                     TLLRRAVQGGARQYALRTDPGDEPPVIAERAEDLDAAESRIVERAGAARGDWASLYLL
                     GPIERSLTGLLMPTSVTPGWLDITAAALTGLAALLFAQGWLWAGLVLVLVSTPLDGAA
                     ERLAALRLQPRRGLGWWSYILPALAAAALVALAWALGRTAGWGCLALAATTLAFLLAL
                     RGETGAGMPASARFFLAERKGMAWLMLPFAAAGLWVTGLGALAGYAAGSFFWAQRQVH
                     RPRD"
     gene            1824..4454
                     /gene="topA"
                     /locus_tag="VF589_02075"
     CDS             1824..4454
                     /gene="topA"
                     /locus_tag="VF589_02075"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX8256396.1"
                     /translation="MKLVVVESPAKAKTIEKYLGGDYRVLASYGHVRDLPPKDGSVDP
                     DNGFDMLWETYADKARQLKAITDEAKKADSLILATDPDREGEAISWHVQEVLQKRRAL
                     PADVSRVTFNAITKATVLEAMKHPRALDEDLIDAYRARRALDYLVGFTLSPVLWRKLP
                     GAKSAGRVQSVSLRLIVDREREIELFRAQEYWSVSATMEADGTEFLARLVRFKGEKID
                     RLTIGEQGTAMAAKAAVEAGRFTVETVETRPLSRNPPPPFTTSTLQQEAARKLGFSAS
                     HTMRIAQQLYEDGSITYMRTDGVDMAPEAISAARKAVAARYDAGYVPDKPRVYTTKAK
                     NAQEAHEAIRPTDFSRDRAGSGDHGRLYDLVFKRALASQMASARLERTTVEMADGTGQ
                     TGLRATGQVVLFPGFLALYEEGKDDEADEESRRLPRLREGDAPAKKSVHAEQHFTQPP
                     PRYSEASLVKKMEELGIGRPSTYAAILQTLKDRDYVRIDKNRFFPEESGRLLTAFLER
                     FFERYVSYDFTAGLEDSLDEISGGRADWQAVLDAFWRDFKPKTAEVMEQKPSEITLAL
                     DEFLAPFLFPDKGDGSDARLCPACGEGRLALRGGRFGAFVACSNYPDCKFTRRFAQGG
                     EEASGEESGPELLGQDPVGGQDVLRKVGRFGPYVEMGEGKEAKRASIPKDAGELDLEL
                     ALRLLSLPRTVGLHPESGNPITASIGRYGPYLAHDGKYAKLGGTAEVFETGMNAAVVK
                     LAEAAAGAGRGRGSREPLKVLGAHPRTELELRLMEGRFGPYVTDGTTNATLPKSLAPD
                     ALTLEEAAQLIDDRAAKGPPPKKGRGRKAAPAKKAGEKKAPAAKKAPAAKKAPAAKKA
                     PAAKKAADRQPRLRSAEPRP"
     gene            complement(4842..7370)
                     /locus_tag="VF589_02080"
     CDS             complement(4842..7370)
                     /locus_tag="VF589_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8256397.1"
                     /translation="MSVSMWQRLGLRPGSSRDEIKRAYARKLKSCNPEDDPDGFKALR
                     QAFELATRYADAVVYQPPEAPGSDEAEDGEDESGGEAGDGGPRHAPAARDMAEPADAR
                     PPADAAETAARAIDLEQRRETEALEAQLGRLNALLTGPDPQPEDVLREAFDKILASPA
                     LQQLATRDQVERWIAWRVASTVPRSDALVEPAILAFRWHDSSERHDRNPAVARVLART
                     EEWRFMNAAATPGDPLHLGWRALTGVSAPEWKLRLEGLSQARAAQVQSLLEIADHRLP
                     GLATAFHPERVQWWQRYPEKPRITFDTLLLAPLVYYLATLVPTTVPIAVHRAVAAALA
                     AAAPYLYFRLVKQRHHAWQTWQRPWPGPWVARGWMAAAALLLPAGMLLPASLAGSLAL
                     AGLTAAIVAWMVAAEPPQWDTNGLGERVRLLVGRLWWPCLFGWMTLLALRGHRADQFL
                     IAVHGGAIIWWRGRDPMLAELERLVPRWRQAAAVALVAAAAGVTMLAAGRLGEGERLY
                     PVATLVLFCGAVLALVWRAEGEANKFPLVLGWLLLATCYMAAFPEPAPDGPQSERAQP
                     VATAQRLVITPNRGAWQRPQCPARPPSRPPGDAPIACSPQLWVFDEDETARALHAGAA
                     GRIVSTLGLTISSSGAVTGCVPAGLQQASVLTAAACDALLTNALFLPGTDLAGLSVAT
                     TMVVEVDWLAGAPAAGSKAGSKAAPQPAPGAKPDAAPPPERVEAAIAPPPPAECPAEP
                     DPPTGPLPLRPCGSMGEWTNAGDYPAEARRRGETGTSRVLVAVTEEGRPSRCTVDQAS
                     GSKALDQATCDLMMKRGRFLPPRDEAGKALPTELRFTMKWVLED"
     gene            complement(7367..9070)
                     /locus_tag="VF589_02085"
     CDS             complement(7367..9070)
                     /locus_tag="VF589_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HEX8256398.1"
                     /translation="MIIGIDLGTTNSAAAIWRGGEAVLIPNRLGQVLTPSAVHLDEGG
                     EVVVGMAARERQASHPRRTATAFKRYVGTNKQTRLGDRNFSAEELSALVLRSLKEDAE
                     AFLGEPVTEAVITVPAYFNDRQRKATRRAGELAGLRVERLVNEPTAAALAYGIHRLEA
                     ESRFLVFDLGGGTFDVSILEIFEGIIEVRASTGDNRLGGEDFNEVLIAMMRERHAKAW
                     GAWGRQDDALYQKLREQAERARRELSRSPEVTMRAVWRDQEFALTVTAEEFEAKVEPL
                     IARMREPVLRSLRDSGLSPDALSEVILIGGSTRMPVVRKAVARMFGRFPNATVNPDEA
                     VALGAAVQAGLKARDAALREVVLTDVCPFSLGVETARDHGSGRYEDGIFTPIIERNTT
                     VPASRSQMFSTMQANQLMVKVRVYQGESRSVVENVLIGELDVPVPRGPAGQGIECRFS
                     YDINGLLEVDVSVPASNERRQLVIFDSETEMDEAELQRRREALAVLKVHPRDEEANRA
                     VLARAERCWQDSLGDTRGYVDQIIGEFKAAVETQDPRTAERAREALSTALDGLEGASF
                     L"
     gene            complement(9222..9773)
                     /locus_tag="VF589_02090"
     CDS             complement(9222..9773)
                     /locus_tag="VF589_02090"
                     /inference="COORDINATES: protein motif:HMM:NF022607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HEX8256399.1"
                     /translation="MTRNKLILIAAAVVALAAGWYFGSPRWTLYQMAEAAEDRDGERL
                     AGYIDFPVLRETSKAQIKAQLAVRVAEAGAKGGAEDGFAALGAMLGMAMIDPMIDGLL
                     TPEAMQAVFARAPEPGAAAQRPFGADASDAEIIQEGLGSFRLRRKGAPADAGDLIFRR
                     HGLGWKLSEIRIPADLMKDAPTP"
     gene            10106..10243
                     /locus_tag="VF589_02095"
     CDS             10106..10243
                     /locus_tag="VF589_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256400.1"
                     /translation="MVKYAEKVASKRAPLLARPQQGVDRATLRTEISQRFSKTLAYLA
                     K"
     gene            10240..10638
                     /locus_tag="VF589_02100"
     CDS             10240..10638
                     /locus_tag="VF589_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX8256401.1"
                     /translation="MTAGPVWLSIEDILDIHAQQLALFGGAGGIRDFGLIESALLRPE
                     NLYHYEGERDVLTLAVRLGIGIAENHGFVDGNKRTGAAAMIELLAVNGYVLTMPNDTT
                     LGRWIEAVIENEMLEQEFIEALSRFTEPWQ"
     gene            10702..>11382
                     /locus_tag="VF589_02105"
     CDS             10702..>11382
                     /locus_tag="VF589_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8256402.1"
                     /translation="MRSIVETARLLPALVAIALAPGAFAPTPSAYAAAPTTAPPAEPA
                     PAISAAARAAWGFDRSDLPPHPGVRFGVLANGMRYAVMRSAAPAGGLSLRLRVDVGAA
                     AERGDQRGFAHLIEHLIFHGTASIPEGALPLMLPRDGLRRWEDFNAYTTFDETVYRLD
                     LAKSDRRARETALTVLREISSNLVFGRKAVAAAKNAVIAEIAERDAAEDAITAAQNAF
                     FFPGSGIAN"
BASE COUNT         1677 a         4210 c         3790 g         1705 t
ORIGIN      
        1 tccggctgcg cttcggcgcg ctgtgccccc atgctcataa cgggaccata tcagttttgg
       61 accggggcga aagggcgctg acgggaacct gccgttaacg cgagtccttt atcggatgaa
      121 ccatggccta tgaccccgga gccctcaacg cctcgctcgc agccgccgtc ggcagcgatc
      181 ccggcctggt gggcgagctt cgcgccgcct tcgtcgaaag cgccgagcgc caggtcgatc
      241 tgatggcgcg cgcccgctgc gacgccaatt ggcagctcgc cgcctcgcgc ctcaagagcc
      301 tcggcgcgag cttcggcgcg atcggcctca tcgcgctggc cgatgaggcg ctcgacggcg
      361 cccccggcga tccggtcgtg ctgcgcaagc tgcgcgccgc cgtcgacgac ttcgcctcga
      421 tgtgagccga accgctttgc aaccttgcta accgccgcct atcctcgccc gttcgtcggg
      481 aggggaatga tttggccttg gccgcactga tctgcgctta tcaggaaagc gacgagccgg
      541 gcggggcgct gcgcgcgacg ctgcccctcg ccgggcgcag cgtggtcgag cgccaggcgc
      601 ggctcgccgc cgcggccgga gccgagcgca tcatcatcct ggtcgaacgg ctgccgcccg
      661 atctcgccgc cgcgatcgac cggctgcgct ccgagggcgt gagcgcgctc gtcgcccgcg
      721 gcgtcgccga agccgccgca tcggtccagc cggccgaccg gctgctgttc gtcgccgacg
      781 ggctgatcgc cgggcagggg catctgagcc ggctcgtcga agcgcaggga acggccgtgc
      841 tcacggtccc cgatcagagc gtcgacgatc gtttcgagcg gatcgacgcc cattcgcgct
      901 gggcaggcct cgccctgctc gacggcgaga tgctgcggcg caccgccgac atgctcgagg
      961 attgggacct gcaatcgact ttgctgcgcc gcgcggtcca gggcggcgcc cgccaatatg
     1021 ccctgcgcac cgatcccggc gacgagccgc cggtcatcgc cgagcgcgcc gaagatctcg
     1081 acgccgccga aagccggatc gtcgagcgcg ccggcgccgc ccgcggcgac tgggcctcgc
     1141 tctatctgct cgggccgatc gagcgcagcc tcaccgggct gttgatgccg acctcggtca
     1201 cgccgggctg gctcgacatc accgccgcgg cgctcaccgg ccttgccgcc ttgctgttcg
     1261 cgcaaggctg gctgtgggcc ggactggtgc tggtgttggt gtcgaccccg ctcgacggcg
     1321 ccgccgagcg gcttgccgcg ctccggctcc agccgcgccg cggcctcggc tggtggagct
     1381 atatcctgcc ggcgctcgcc gccgccgccc tggtcgcgct ggcctgggcg ctcggccgca
     1441 ccgccggctg gggctgcctc gcactcgccg cgaccacgct cgctttcttg ctcgcgctgc
     1501 gcggcgagac cggcgccggg atgccggcga gcgcccgctt cttcctcgcc gagcgcaagg
     1561 gcatggcctg gctgatgctg cccttcgccg ccgccggcct gtgggtgacc gggctcggcg
     1621 cgctcgccgg ctatgccgcc ggctccttct tctgggcgca gcgccaggtc catcgccctc
     1681 gcgattgact tggccccctc cgctcgggga gagggcggag ggttcaacat catcactttt
     1741 gggccatggc cgatcgcgcg cttgacgggc cgccgtcatg gtccccaccc tgtacgcgta
     1801 cgcgtgaaga aagtttgccg atcatgaagc ttgtcgtcgt cgaatcgccc gccaaggcga
     1861 aaaccatcga aaaatatctg ggcggcgatt atcgcgtgct cgcctcctac ggccatgtcc
     1921 gcgatctgcc gcccaaggac ggctcggtcg atcccgacaa cggcttcgac atgttgtggg
     1981 agacctatgc cgacaaggcc cgccagctga aggcgatcac cgacgaggcg aagaaggccg
     2041 attcgctgat cctcgcgacc gaccccgacc gcgaaggcga ggcgatcagt tggcacgtgc
     2101 aggaggtgct gcaaaagcgc cgcgcgctgc cggccgacgt gtcgcgggtg acgttcaacg
     2161 ccatcaccaa ggcgaccgtg ctcgaggcga tgaagcatcc gcgcgcgctc gacgaggatc
     2221 tgatcgacgc ctatcgcgcc cgccgcgcgc tcgattacct ggtcggcttc actttgtcgc
     2281 cggtgctgtg gcgcaagctg cccggcgcca agtcggccgg ccgggtccag tcggtgtcgc
     2341 tgcggctgat cgtcgaccgc gagcgcgaga tcgagctgtt ccgcgcccag gaatattgga
     2401 gcgtctccgc gaccatggag gccgacggca ccgagttcct cgcccgcctg gtccgcttca
     2461 agggcgagaa gatcgaccgg ctgacgatcg gcgagcaggg caccgccatg gccgccaagg
     2521 cggccgtcga ggcggggcgg ttcaccgtcg agacggtcga gacgcggccg ttgagccgca
     2581 acccgccgcc gcccttcacc acctcgaccc tgcagcagga ggcggcgcgc aagctcggct
     2641 tttccgccag ccacacgatg cgcatcgccc agcagctcta cgaggacggg tcgatcacct
     2701 atatgcgcac cgacggcgtc gacatggcgc ccgaggcgat ctcggccgcg cgcaaggcgg
     2761 tcgcggcgcg ctacgatgcc ggctacgtcc ccgacaagcc gcgcgtctac accaccaagg
     2821 ccaagaatgc gcaggaagcg catgaggcga tccggccgac cgacttcagc cgcgaccgcg
     2881 ccggatcggg cgaccatggc cggctctacg acctcgtctt caagcgcgcg ctggccagcc
     2941 agatggcctc ggcgcggctc gagcgcacga ccgtggaaat ggccgacggc accggccaga
     3001 ccggcctccg ggcgaccggc caggtcgtgc tctttccggg cttcctggcg ctctatgaag
     3061 agggcaagga cgatgaggcc gacgaggaga gccggcgcct gccgcgcctg cgcgaaggcg
     3121 acgccccggc caagaagtcg gtccatgccg agcagcattt cacccagccc ccgccgcgtt
     3181 actccgaggc cagcctggtc aagaagatgg aggagctcgg catcgggcgg ccgtccactt
     3241 acgccgcgat cctgcagacg ctgaaggacc gcgactatgt ccggatcgac aagaaccgct
     3301 tctttcccga ggagagcggg cggctgctga ccgccttcct cgagcgcttc ttcgagcgct
     3361 acgtctccta cgatttcacc gccggactcg aggacagcct tgacgagatt tccggcgggc
     3421 gcgccgattg gcaggcggtg ctcgacgcct tctggcgcga cttcaagccc aagaccgccg
     3481 aggtgatgga gcagaagccg tccgagatca cgctggcgct cgacgaattc ctcgcgccct
     3541 tcctgttccc cgacaagggc gacggcagcg atgcgcggct gtgcccggcc tgcggggagg
     3601 ggcggctggc gcttcgcggc ggccggttcg gcgcgttcgt cgcttgctcc aattatcccg
     3661 attgcaagtt cacccgccgc ttcgcccagg gcggcgagga agccagcggc gaggagagcg
     3721 ggccggagct gctcggccag gacccggtcg gcggccagga cgtgctgcgc aaggtcggcc
     3781 ggttcggccc ctatgtcgaa atgggcgaag gcaaggaggc caagcgcgcc tcgatcccca
     3841 aggatgcggg cgagctcgac ctcgagctgg cgctgcgcct gctgtcgctg ccgcgcacgg
     3901 tcggcctcca ccccgagagt ggcaacccga tcaccgcctc gatcggccgc tacggcccct
     3961 atctcgcgca tgacggcaaa tatgcgaagc ttgggggcac cgccgaggtg ttcgagaccg
     4021 gcatgaacgc ggccgtcgtc aagctggccg aggctgccgc cggcgccggc cgcgggcgcg
     4081 gctcgcgcga gccgttgaag gtgctgggcg cgcacccgcg caccgagctg gagctccgcc
     4141 tgatggaggg ccggttcggc ccctatgtca ccgacggcac gaccaacgcg accttgccca
     4201 agtcgctcgc gcccgacgcc ctcacccttg aggaagccgc ccagctgatc gacgaccgcg
     4261 ccgccaaggg cccgccgccc aagaagggca ggggcaggaa agccgcgccc gccaagaagg
     4321 cgggtgagaa gaaggcgcct gcggcgaaga aggcacccgc agcgaagaag gcgccggccg
     4381 cgaagaaggc gccggccgcg aagaaggcgg ccgatcgtca gcctcgcctg aggtccgcgg
     4441 agccgcggcc ctaagaggct ctttgcaccg tccattccgc tccgcgccca agccggctgc
     4501 gccgcgcgcc aatctcgcga gatcgtgagc ttcgcgccgt cagcaagacc tttcatcacc
     4561 ccaggatgcg aactcgcgag aggccggcgt ccgtctcccg ccgaagttcg ttttagcgtg
     4621 accttagtgt gaccttccgc cgcgccgccc ggcgatcgaa cgctggcggg ctcggcccgt
     4681 ctccccggcg ccgcaccacg atcgggttgg cagccgtgaa cttcgactcg tgcccagttc
     4741 cgccccggag caggggaggg cgcgcctctc ggcaagcctc acttcctcaa cttatttccg
     4801 ccacctcggc tggttcgacg ccgccgccgg cctggcccgc ttcaatcctc caacacccat
     4861 ttcatcgtga agcgcagctc ggtggggagc gccttgccgg cctcgtcgcg cggtggaagg
     4921 aagcggccgc gcttcatcat caaatcgcag gtcgcctggt cgagcgcctt cgatccgctg
     4981 gcctggtcca ccgtgcaccg gctgggccgg ccttcctccg tcacggccac cagcacccgg
     5041 ctcgtgccgg tctcgccgcg acggcgcgct tcggccggat agtcgccggc attggtccac
     5101 tcgcccatcg atccgcacgg ccgcagcggg agcgggccgg tcggcgggtc gggctcggcc
     5161 gggcattccg ccggcggcgg cggcgcgatc gcggcctcga cccgttcggg cggcggcgcc
     5221 gcgtcgggct tggcccccgg cgccggctgc ggagccgcct tcgaacccgc cttcgacccc
     5281 gccgccggcg cccccgccag ccagtcgacc tccaccacca tcgtagtcgc caccgacaag
     5341 cccgcaaggt cggtgcccgg caggaacagc gcgttggtga ggagggcgtc gcaggccgcc
     5401 gccgtcagca ccgacgcctg ctggagaccg gccggtacgc atccggtgac ggctccggag
     5461 ctcgatatcg tgagcccgag cgtcgagacg attcgtcccg ccgcgccggc gtgcagggcg
     5521 cgcgcggtct cgtcctcgtc aaacacccac agctgcggcg agcaggcgat cggcgcatcg
     5581 cccggcggcc ggcttggcgg gcgcgccgga cattgcggcc gctgccaggc tccccggttc
     5641 ggggtaatga cgaggcgctg ggcggtggcc accggctgcg cgcgctcgct ttgcggccca
     5701 tcgggcgccg gctcggggaa ggccgccatg tagcaggtgg cgagcagcag ccagcccagc
     5761 accagcggga atttgttggc ctcgccctcg gcgcgccaga ccagcgccag caccgccccg
     5821 cagaacagga ccagcgtcgc gaccgggtag aggcgctcgc cctcgccgag gcggcccgcg
     5881 gccagcatcg tcactcccgc cgccgccgcg accaaggcga ccgccgccgc ctggcgccat
     5941 ctcggcacca ggcgctcgag ttcggcgagc atcgggtcgc ggccccgcca ccagatgatg
     6001 gcgccgccgt ggaccgcgat caggaactgg tccgcgcgat gcccgcgcag cgcgagcagg
     6061 gtcatccagc cgaacaggca cggccaccac aagcggccga ccaacagccg cacccgctcc
     6121 cccaagccgt tcgtgtccca ttgcggcggc tcggccgcca ccatccacgc cactatggcg
     6181 gcggtgagcc cggccagcgc caggctgccg gcgaggctgg ccgggagcag catcccggcc
     6241 ggcagcagca aggccgccgc ggccatccat ccgcgcgcca cccagggccc gggccacggc
     6301 cgttgccacg tctgccaggc atggtggcgc tgcttgacga ggcggaagta gagatagggc
     6361 gccgccgccg cgagcgccgc cgccaccgcc ctgtggacgg cgatcggaac cgtcgtcgga
     6421 accagggtgg ccagatagta aacgagcgga gccagcagca gcgtatcgaa ggtgatccgc
     6481 ggcttctccg ggtaacgctg ccaccattgg acgcgctccg gatggaacgc ggtggccagc
     6541 cccggcagcc gatggtccgc gatttcgagc aggctttgca cctgcgccgc ccgcgcctgc
     6601 gaaagtccct ccagcctcag cttccactcc ggcgcggaca ctccggtcag cgctcgccag
     6661 ccgaggtgca gcgggtcgcc cggcgtcgcc gcggcattca tgaagcgcca ttcctcggtc
     6721 cgggcgagca ccctcgccac cgccgggttg cggtcgtgcc gctcggagga atcatgccac
     6781 ctgaaggcga ggatggcggg ttccaccagc gcgtccgacc gcggcaccgt cgaggcgact
     6841 cgccaggcga tccagcgttc gacctggtcg cgcgtcgcga gttgctggag cgcgggcgaa
     6901 gccaggatct tgtcgaacgc ctcgcgcagg acatcctccg gctgcgggtc ggggccggtg
     6961 agaagcgcgt tgagccgccc aagctgcgcc tcgagcgcct cggtttcccg cctttgctcc
     7021 agatcgatcg cgcgcgccgc cgtttccgcc gcgtcggcgg gcggccgcgc gtcggccggc
     7081 tcggccatat cgcgcgccgc cggggcgtgc cgcggccctc cgtcgcccgc ctctccgccg
     7141 ctctcgtcct ccccgtcctc cgcctcgtcg ctgcccggcg cttccggcgg ctggtagacg
     7201 acggcgtcgg cgtaacgcgt ggccagctcg aacgcctggc ggagcgcctt gaagccgtcc
     7261 ggatcgtcct ccggattgca gctcttcagc ttgcgggcat aagcgcgctt gatctcgtcg
     7321 cggctgctcc ccggccgcag gccgagccgc tgccacatgc tcacgctcac aggaagctgg
     7381 ccccttcgag cccatccagg gcggtcgaca gcgcctcgcg cgcgcgctcc gcggtccgcg
     7441 gatcctgcgt ctcgaccgcc gctttgaact caccgataat ctggtcgaca tagccgcgcg
     7501 tgtcgccgag cgaatcctgc cagcagcgtt cggcgcgcgc cagcaccgcc cggttcgcct
     7561 cctcgtcgcg cgggtgcacc ttcagcacgg cgagcgcctc gcggcgccgc tggagctcgg
     7621 cctcgtccat ttcggtttcc gaatcgaaga tgacgagctg gcgccgttcg ttggacgccg
     7681 gcacgctgac atcgacctcg agcaggccat tgatgtcgta ggagaagcgg cattcgatgc
     7741 cctggccggc cggcccgcgc ggcaccggca cgtccagttc gccgatcagc acattctcca
     7801 cgaccgaccg ggattcgccc tgatagaccc gcaccttcac catcagctgg ttggcctgca
     7861 tcgtgctgaa catctgcgac cggcttgccg gcaccgtggt gttgcgttcg atgatcggcg
     7921 tgaagatgcc gtcctcatag cggccgctgc catggtcgcg ggcggtctcg actccgagcg
     7981 agaaggggca gacgtcggtc aggaccactt cgcggagcgc cgcgtcccgc gccttgaggc
     8041 cggcctgcac ggcggcgccg agcgctaccg cctcgtcggg gttgacggtg gcgttgggga
     8101 agcggccgaa catccgcgcg accgccttgc gcaccaccgg catgcgcgtc gagccgccga
     8161 tcaggatcac ctcgctgagc gcgtccgggg agaggccgct gtccctcagc gagcgcagca
     8221 ccggctcccg catccgggcg atcagcggct cgaccttcgc ttcgaattcc tcggcggtga
     8281 cggtgagggc gaattcctgg tcgcgccaca ccgcccgcat cgtcacctcc gggctccggc
     8341 tgagctcgcg ccgcgcccgc tccgcctgct cgcgcagctt ctggtagagc gcgtcgtcct
     8401 gccgccccca ggcgccccag gccttggcgt ggcgctcccg catcatcgcg atcaggacct
     8461 cgttgaaatc ctcgccgccg agccggttgt cgccggtgga ggcgcgcact tcgataatgc
     8521 cttcgaagat ttccaaaatc gagacgtcaa acgtgccgcc gcccaggtcg aagaccagga
     8581 agcgggattc cgcctccagc cggtggatgc cgtaggccag cgccgcggcc gtcggctcgt
     8641 tcaccagccg ctccacgcgc agcccggcga gctcccccgc ccgccgcgtg gccttgcgct
     8701 ggcggtcgtt gaaataggcc ggcaccgtga tcaccgcctc ggtcaccggc tcgccgagga
     8761 aggcctcggc atcctccttc agcgatcgca gcaccagcgc cgaaagctcc tccgcgctga
     8821 aattgcggtc gccgagccgg gtctgcttgt tggttccgac atagcgcttg aaggcggtgg
     8881 cggtgcgccg cgggtggctc gcctgccgct cgcgcgcggc catgccgacg accacctcgc
     8941 cgccctcgtc gagatggacc gccgacggcg tcagcacctg gcccagccgg ttgggaatga
     9001 gcacggcctc accgccgcgc cagatcgcgg cggcactgtt ggtcgtgccg agatcgattc
     9061 cgataatcat gaagccccac ttgcctacgc gctccgcagg tctttcagaa tgcggcgaaa
     9121 ctggcaacat gccgcggccg cgcgcgcgcc ttttcccgcc ggccgctccg gtcgcgcccg
     9181 gtcttcgcca tgcccggccc gcgttcagcc cggaaacccg gctaaggcgt cggcgcatcc
     9241 ttcatcagat cggccggaat gcggatctcg gacagcttcc agccgagccc gtggcgccgg
     9301 aagatcaggt cccccgcgtc cgcgggcgcg cccttgcggc gcagccggaa gctgcccagg
     9361 ccctcctgga tgatctcggc gtcggacgcg tcggcgccga acgggcgctg cgccgcggcg
     9421 cccggctccg gcgcgcgcgc gaacaccgct tgcatcgcct cgggcgtcag caggccatcg
     9481 atcatcgggt cgatcatcgc catgcccagc atcgcgccga gcgcggcaaa gccgtcctcc
     9541 gcgccgccct tcgcgccggc ttcggccacg cgcaccgcca attgcgcctt gatctgcgcc
     9601 ttcgacgtct cgcgcaggac tggaaagtcg atatagcccg ccagcctttc gccgtcgcga
     9661 tcctcggcgg cctcggccat ctgatagagc gtccatcgcg gcgagccgaa ataccagccc
     9721 gccgccagcg cgacgacggc ggccgcgatc aggatcagct tgttgcgcgt catcgtcttc
     9781 gcccccgccg ctcgcccgtt cgcccacaga cctagccggg cttcatcccg cgcgccagcc
     9841 agaggctcca ccccgccatg cgcggcgcct tcgtcgcgcc catcaataca ccggattcgc
     9901 taattggcag aatgcttgtg ccgctgacct gagggactac tttcaaactc acaacgaaat
     9961 gtacatcacg cggtcaacgg gaagcgtcta caagcgagcc tcaggcgcag cggtgaaccc
    10021 aaacgagaaa ccaacggcca ccgctaacgt tgacgatacc gtcataattg caatatacac
    10081 gccgatgcca ggaaggacgc accccatggt caagtacgct gagaaggtcg ctagcaagcg
    10141 cgctccgctg ctggctcgcc cgcagcaggg tgtcgatcgc gcgaccctac ggaccgagat
    10201 aagccagcgg ttctccaaga cgctcgccta cctcgcgaag tgaccgccgg gcctgtttgg
    10261 ctctccatcg aggatatcct agatattcac gcgcagcagc tggccctgtt tggaggcgcc
    10321 ggcggcatcc gcgactttgg attgatcgag agcgccctcc tcaggcctga gaatctctat
    10381 cactacgagg gcgagcgtga cgttctcacg ctcgccgttc gtttgggcat cggcatagcc
    10441 gaaaaccacg gattcgtaga cggcaacaag cgaaccggcg ccgcggccat gatcgaattg
    10501 ttggcggtga acggctatgt gttgaccatg ccgaatgaca cgacgctcgg ccgctggatc
    10561 gaagctgtca tcgagaacga gatgctggaa caggagttca tcgaggctct gtcccgtttc
    10621 acggagcctt ggcagtagcc ggtcgcaatg cgcacccaca tatcgttcta gatcatgcgc
    10681 tttgccgacc cgggagcgaa ggtgcgatcg atcgttgaaa cggccaggct gttgccggcc
    10741 ctggtcgcca tcgcgctggc ccccggcgcc ttcgccccca cgccctcggc ctacgcggcc
    10801 gcgccgacca ccgcgccgcc cgccgagccc gcgcccgcaa tctccgccgc cgcccgcgcc
    10861 gcctggggct tcgaccgcag cgatcttccg ccccatcccg gcgtccgctt cggcgtgctg
    10921 gccaacggca tgcgctatgc ggtgatgcgc agcgccgccc cggccggcgg gctctcgctg
    10981 cggctccgcg tcgatgtcgg cgccgccgcc gagcgcggcg atcagcgcgg cttcgcccac
    11041 ctcatcgagc atctgatctt ccacggcacc gccagcatcc ccgaaggcgc gctgccgctg
    11101 atgctcccgc gcgacgggct ccggcggtgg gaggatttca acgcctatac aacgttcgac
    11161 gagaccgttt accggctcga tctggcgaaa tccgaccggc gggcaaggga gacggccctg
    11221 acggtgctgc gggagatttc cagcaacctc gtcttcggcc gtaaggccgt cgcggccgcg
    11281 aagaatgcgg tgatcgccga gatcgcggag cgggacgctg ccgaggacgc gattacggcg
    11341 gcgcaaaacg cgttcttctt tcccggttcg ggaatcgcca ac
//
LOCUS       DASVRK010000058         9942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_259101, whole genome shotgun sequence.
ACCESSION   DASVRK010000058 DASVRK010000000
VERSION     DASVRK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259101"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VF589_02110"
     CDS             <1..870
                     /locus_tag="VF589_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX8256403.1"
                     /translation="LDSVDRAWAWLADADDHEVLKAMRSHAVQPRRRSWTSWRPAAAA
                     AAAILLVLSAGMLLFLTPDWRGPATNGDAAGDPGIRYATAHGQVRRIELPDGSVMTLD
                     AQSVAVARSGAAGRSVELARGRALFEVAADASRPFSVTAADRRVVAVGTRFDVGLAPG
                     TLTVNLLEGAVTVGPLAEDVTPVRLKPGQQFVERGGRARIRNLGAEVENAVGWKSGLL
                     HFDDDTLGEAVAEVNRYANVQIAIRDADVAAMRVSGMFRAGDSEAFVATVAELRPVRG
                     VRSGDRIELVPAE"
     gene            969..4079
                     /locus_tag="VF589_02115"
     CDS             969..4079
                     /locus_tag="VF589_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8256404.1"
                     /translation="MRGRAGFALFAASAAAIAAPAQAAVRSADFAIPAGPLSATLNEL
                     GRQAGVNLLFRPEAVRGLSSPAVRGRMSVEAALRRLLGGTNLTIRRAASGAFIVEGRA
                     GPAAAAPAAARSKPTPSPPAEEPPVPEILIIGRRTQNVDIRRQVNDVQPYRVATGRQI
                     VQAHRDTLDQFFQSRVTSNTQVVSPSLFERGETLSEIDLRGLGSDGTLVLVDGRRLPS
                     IPRWTTFAFEPDPEGFRFRQPDVNAIPLHAIERVETLTGTAGGIYGFGALGGVVNVVL
                     KRDYRGVELHGTAGISSRGDAGRLSLEGRAGFTPNDGRTDVMLYFSHAQSDPLRVGDR
                     HYAVRDLEQTLRFVPERFLAVPPTSNSVLVTPLTGNSLTFREEYGGGPLGAGYTFLPA
                     GFAGSPAEMVRALRQRAGQFDFTLSEVRAASGLGSAPELTAGIVNVRHRFGAGLEGYF
                     DALLMRNSGRHTSYPGAGTMFVPPDSTINPFNESILLRFPLPEASDHHRRRFTTSRYT
                     AGMVGDLPLRGWRATAEATFGSMRADVLQKTEGSTTVFLGPPLGPDLNPLGNWEEFQR
                     NAVIDRFSTETEAHTRSRYREQSLRLAGPLLRAPGGLVNLSLLAERRSERVPAFTTVT
                     RFESAFGGGEIEVPTGARGIVTTSFYGELRAPLLGHEGAVPLLDGLELQLAARHDRLR
                     TSFDPHPEDPETDEIAKFAFRGTSFTAGAKAFPLPWLMLRGSYATGEAQPPPMELLIR
                     EEGIYDGLGATDPQRGDSPVGSEAPFLRRFGGSPDLGPARARTLSIGAVLNPSGERGP
                     RVSLDYSRITKSREGALLHGQYVLDHEGRWPERVERGPLSDQDRALGYTGGVIEVLDE
                     SAINLGGVRVESLDGQFDWAVPFVGGRLRLYGAATLQISNKQRQLLDAPLEQVGFRQG
                     PLKWRANVGADWSAGRTTLGANVQYFSRYRIQDAFLDSITDDVSVQGSPWVKSQAYLD
                     LHASRSFALRNAGPLRRFSIDFAIVNVLDTAPPREAGYFYNGPGYSRYGDPRRRRFEV
                     VLSSGF"
     gene            complement(4063..4842)
                     /locus_tag="VF589_02120"
     CDS             complement(4063..4842)
                     /locus_tag="VF589_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8256405.1"
                     /translation="MITIVADRRMEARRVPVAGAAFSHREALASPFVTPSAAPHALVH
                     ILDFDSELREVLAGLFRSAGLEARTYGDLDAFSNTRQSDVANCLVIDARLAAMGGSGK
                     EPGLQPLGTDMPIIVTASRADVRTAVQAMKAGAVDFLEKPFREHDMLEAVGAAIRVDR
                     ARRSTHSRCAELRARFQTLTRREREVMALVTAGKMNKQAAGDLGLSEITVKVHRGSVM
                     RKMRAATLADLVRMADTLAAAASSANELAFAGACSGLRTRN"
     gene            complement(5338..6939)
                     /locus_tag="VF589_02125"
     CDS             complement(5338..6939)
                     /locus_tag="VF589_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8256406.1"
                     /translation="MPADAAPRPAGPQAALEPLPRDSVLLLATALSGSLLVNFSGQFV
                     TSNLADIQGAIGASADEASWLTTGYTMALLAGIVVSSPLIASFGLRRCLAAAALVFAF
                     AALGCAAAPPLPVMIVLRLLQGSAAGGFGPIAFAATFMTAAGPRLPFGLSLLALFLLL
                     PTTLGPAASGLIEDRVGWEALFLVQAAIGSGLAAAALLFIPRAPIAWDALRRDWAALL
                     LLCLALAATMLVLGQGTRRYWLDSDLIRWSLAIGAGAWAGFLVTAWRSPRPVLSLALL
                     ARLSFIAPITLNLLFRAGFASTAFLAPQLLAMVQGYRPLELANLFLWAAVPQLLAFPI
                     AWWLLHRVDARLIIGAGLILFGLAALLASGSTSLAAAEQFRISYALAGAGQVLFLVPN
                     LVAGGSPLKPADGPTASLMFNVTTAGGTNLGIALATELVTERQKFHVAALAESAAAYG
                     SNLDRLDALAGSLAARAGDDALAAARTIATLAGALHREAWVLAFNDGFLLVGAMLIAS
                     AAILLLFPRQPPLAGSPRTATGSAR"
     gene            complement(6971..7387)
                     /locus_tag="VF589_02130"
     CDS             complement(6971..7387)
                     /locus_tag="VF589_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ohr family peroxiredoxin"
                     /protein_id="HEX8256407.1"
                     /translation="MDQSDKVIYTARTHTIGGRQGQSRSDDGNLEVRLATPGTHAVGT
                     NPEQLFAAGWSACFEAAVAEVARARRVKLPDGVTIDAEVDLRKGDSSYSIAARLNATI
                     PGVDRALAEELLEEADQTCPYSKATRGNIEAVIRLV"
     gene            7683..7919
                     /locus_tag="VF589_02135"
     CDS             7683..7919
                     /locus_tag="VF589_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX8256408.1"
                     /translation="MPHINVQVTREGTAPGVDRVTPEQKAAIYKGMSQVLLDVLGKPL
                     DRTVVIFQEVELEDWGRGGMPIAEFRKLGAASGG"
     gene            8060..9154
                     /locus_tag="VF589_02140"
     CDS             8060..9154
                     /locus_tag="VF589_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8256409.1"
                     /translation="MGSEAVDEDGPFLELADSVPVMLWRINSTFDCDWANKAWFEFTG
                     GTLPQQTGFAWLDLVHPDDSDRVVEAFGRAFEAREKVSVEFRIRRVDGRYRWVLDSGS
                     PYFRDGLFAGFIGSCVDITERKEAEQRTEALAAEMVRLSRAEALSSLATAIVHDLAQP
                     LQAIGAYAEGVAEILANRGPSSEEAIAGVNEIREAAARAAGIVRNCRSLAAGRKSERV
                     RADVAAVLRGAEPWIRSHPDAAGVSIDWNLASGLEAELSPLEIEQLLINLASNALEAM
                     RGMPGAELRIAAARWGGLAIVSVADTGRGVPPDMRKIIFRSLVTTKPKGMGIGLYVCH
                     AIATAHGGRIWVEAGAEGGAVFKFTLPLTP"
     gene            9548..>9942
                     /locus_tag="VF589_02145"
     CDS             9548..>9942
                     /locus_tag="VF589_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8256410.1"
                     /translation="MLLRRWAALLSLLIAWSLAVHIPSAYAAEAERIVAIGDLHGDHA
                     AWRQIAAAAGLIDQAGRWAGGRAILVQTGDVTDRGPDSLKIIRDLRRLQSEAQRAGGR
                     VIAVVGNHEAMNVTGDLRYVHPGEYAAFRG"
BASE COUNT         1568 a         3352 c         3521 g         1501 t
ORIGIN      
        1 ctcgacagcg tcgaccgcgc ctgggcgtgg ctggcggacg ccgacgacca tgaggttctg
       61 aaggcgatgc ggagccatgc ggtgcagccg cggcggcgca gctggacgag ctggcgccct
      121 gccgccgccg ccgccgccgc gatcctgctc gtgctcagcg caggcatgct gctgttcctc
      181 acgccggact ggcgcgggcc ggcgacgaac ggcgacgccg ccggcgaccc gggcatccga
      241 tatgccacgg ctcatgggca ggtaaggcgg atcgagcttc ccgacgggag cgtcatgacg
      301 ctcgacgcgc aaagcgtcgc ggtcgcgcga tcgggcgctg cggggagatc ggtggagctt
      361 gcccgggggc gcgcgctgtt cgaggtcgcg gccgatgctt cacggccctt ctccgtcacc
      421 gccgcggatc gccgggtggt ggcggtggga acccgcttcg acgtgggcct ggccccgggg
      481 acgctgaccg tgaacctgct ggagggcgcc gtcactgtcg gtccgctggc cgaggatgtc
      541 accccggtga ggctgaagcc cggccagcaa ttcgtcgagc ggggcggcag ggcgaggatc
      601 cgcaacctgg gcgcggaggt cgagaatgcg gttggctgga aaagcggcct gctccatttc
      661 gacgacgaca cgcttggcga ggcggttgcc gaggtgaacc gctatgcgaa cgtccagatc
      721 gcgatccgcg acgcggatgt cgccgcgatg cgtgtgtccg gcatgttcag ggcgggcgac
      781 tcggaagcgt tcgtcgccac cgtcgcggag ctgcgcccgg tgcggggagt acgcagcggc
      841 gacaggatcg aactcgttcc cgccgaataa taaagccgga gcagccacct gcggctttgc
      901 cgtcgccccc gtgaccgcgg cccccgtttc tggtaggtca cgcggtcgct gtgggggtgg
      961 ctatgctgat gagggggcga gccggatttg cgcttttcgc ggcctcggcg gcggcgattg
     1021 ccgctccggc gcaggccgcc gtccggtcgg ctgatttcgc gatcccggcc ggaccgctgt
     1081 ccgcgacgtt gaacgagctc gggcggcagg ccggcgtcaa cctcctgttc cggccggagg
     1141 cggtgagggg gctttccagc ccagcggtcc gcgggagaat gtcggtcgag gccgcgctcc
     1201 ggcgcctgct cggcggcacc aacctcacca taaggcgcgc ggcctcgggc gcgttcatcg
     1261 tcgaggggcg ggccggtccc gccgcggccg ctccagccgc ggctcgcagc aagccgactc
     1321 cttccccgcc cgcggaggaa ccgcctgttc ccgagatcct gatcatcggc aggcgtacgc
     1381 agaacgtcga cattcgtcgc caggtgaacg acgttcagcc ctaccgagtc gcgaccggcc
     1441 ggcagatcgt gcaggcgcat cgcgacacgc tcgaccagtt tttccagagt cgcgtcacct
     1501 ccaacaccca ggtcgtctcc ccctcgctgt tcgaacgcgg cgagacgctg tccgagatcg
     1561 atctgcgcgg gctcggttcg gacggcacgc tggtgctggt ggacggacgg cggctgccga
     1621 gtataccgcg ctggacgacc ttcgcgttcg agcccgaccc cgaaggtttt cgcttccgtc
     1681 agccggacgt caacgccatc ccgctccacg cgatcgagcg agtcgaaacc ctcaccggca
     1741 ccgccggcgg gatttacggc ttcggcgcgc tcggcggcgt ggtgaacgtc gtgctcaagc
     1801 gcgactatcg cggcgtcgag ctccacggca ccgccggcat cagctcgagg ggcgatgcgg
     1861 ggcggctgag cctcgagggc cgcgccggct tcacgccgaa tgacgggcgt accgacgtga
     1921 tgctctattt cagccacgcc caatccgatc ccctgcgggt cggcgaccgc cattatgcgg
     1981 tgcgggacct ggagcagacg cttcgcttcg ttcccgagcg atttctggcg gtgccgccga
     2041 cgtcgaactc cgtcctggtg acgccgctca ccggaaattc gctcacgttc cgggaggaat
     2101 atggcggcgg gccgctcggg gccggctaca ccttcctccc cgccgggttc gcggggtccc
     2161 cggcggagat ggtccgcgcg ctaaggcagc gggcgggcca gttcgacttc accttgtcgg
     2221 aggtccgggc ggcgagcggt ctcggctcgg cgccggaact gaccgccggg atcgtcaatg
     2281 tgcggcaccg cttcggcgcc ggcttggaag gctatttcga cgcactgctg atgcgcaaca
     2341 gcgggcgcca caccagctat cccggtgcgg gcacgatgtt cgtaccgccc gactcgacga
     2401 tcaacccgtt caacgaaagc attctgcttc gctttccgct tccggaagcg agcgaccacc
     2461 atcgccgccg gttcaccacc tcccgctaca cggccggcat ggtaggcgac cttcccctga
     2521 ggggatggcg ggctacggcc gaagcgacgt ttgggtcgat gcgcgcggac gtgctgcaaa
     2581 agaccgaagg gtcgaccacg gtgttcctcg gtccgccgct ggggccggac ctcaacccgc
     2641 tcggcaattg ggaggagttt cagcgcaacg ccgtgatcga ccgcttctcg acggagaccg
     2701 aggcccatac gcgcagccgc taccgtgagc agtcgctgcg gctggcgggc ccgctgctga
     2761 gagcgccggg cggcctcgtc aatctgtcgc tgctcgccga acggcgcagc gaacgggtgc
     2821 cggcgttcac gacggtcacc cgcttcgagt cggcgttcgg cgggggggaa atcgaagtac
     2881 cgaccggcgc cagaggcatc gtcaccacat ccttttacgg ggagctgcgc gctccactcc
     2941 tcggccatga gggtgcggtc ccgctattgg acggcctcga actgcagctc gccgcccgcc
     3001 acgatcgcct gcggacaagc ttcgatcccc atccggaaga ccccgaaacc gatgagatcg
     3061 cgaagttcgc gtttcgcggc acgtccttca cggcgggggc gaaggccttt ccgctcccct
     3121 ggctgatgct gcgcgggagc tacgccaccg gcgaggcgca gccgccgccg atggagctgc
     3181 tcatccgcga agaggggatt tacgacggcc tgggcgcgac cgacccgcag cggggcgaca
     3241 gcccggttgg atcggaagcg cctttcctgc gcaggttcgg cggctcccca gacctcggac
     3301 ccgcccgggc caggaccttg tcgatcggcg cggtgctcaa tccctccggt gagcgcggac
     3361 ccagggtatc gctcgattat tcccggatca cgaaatcgcg cgagggggct ctgctgcacg
     3421 gccaatatgt gctggaccat gagggccggt ggccggagcg cgtggagcgg gggcctttgt
     3481 ccgatcagga tcgggctctg ggctatacgg gcggggtgat cgaagtgctc gacgaaagcg
     3541 cgatcaatct gggcggtgtg cgagtcgaga gcctcgacgg ccagtttgac tgggcggtcc
     3601 cgttcgtggg cggaagattg cgcctgtacg gcgccgccac gctgcagatc agcaacaagc
     3661 agcgacagct gctcgatgcg ccgctggagc aggtcgggtt ccgccagggg ccgctcaaat
     3721 ggcgcgccaa tgtcggggcg gactggtcgg ccggccgcac cacgctcggc gcgaacgtcc
     3781 aatatttcag ccgctaccgc attcaggacg ccttcctgga ttcgatcacc gacgacgtct
     3841 cggtccaggg ctcgccatgg gtgaaatcac aggcttatct cgacctgcat gcgagccgta
     3901 gtttcgcgct ccgcaatgcc gggccgctgc ggcgcttcag catcgacttc gcgatcgtca
     3961 acgtgctcga cacggcgccg ccccgggagg ccggctattt ctacaacggg ccgggatata
     4021 gccgctacgg cgacccgcgc cggcgccggt tcgaggtcgt gctcagttcc gggttctgag
     4081 gcctgagcag gctccggcga aggcaagctc gttggcggag cttgcggccg ccgcgagcgt
     4141 gtcggccatg cgaaccaggt cggcgagcgt ggccgcccgc atcttgcgca tcaccgatcc
     4201 gcgatggacc ttgacggtga tctcgctgag cccgagatct cccgccgcct gcttgttcat
     4261 cttgcccgcg gtgacgagcg ccatgacctc gcgctcgcgc cgggtgaggg tctggaagcg
     4321 ggcgcgcagc tcggcgcagc gggagtgggt ggagcgccgg gcgcggtcga cccggatggc
     4381 ggcgccgacg gcctcgagca tgtcgtgctc gcgaaacggc ttttcgagaa aatcgacggc
     4441 gccggctttc atcgcctgca cggccgtgcg gacatcggcg cggctcgcgg tgacgatgat
     4501 cggcatgtcg gtcccgagcg gttgcagccc gggttccttc ccgctgccgc ccatcgcggc
     4561 cagccgggca tcgatgacga ggcagttcgc cacgtccgat tggcgcgtgt tcgagaatgc
     4621 gtcgaggtcg ccataggttc gagcctccag ccccgcggac cggaagagac cggccaagac
     4681 ctccctcagc tcgctgtcga aatcgaggat gtggacgagc gcgtgcggcg ccgcgctcgg
     4741 cgtcacgaac ggcgatgcca acgcctcgcg gtgcgaaaat gcggcgccgg ccaccgggac
     4801 ccggcgcgct tccatccgtc tgtcggccac gatcgtgatc atgttcctcc tcctgttccg
     4861 ctcccgccaa aagacgcatc gaaaccggaa atccccaccg gctattttca tgaatcggcg
     4921 ggggagggcg gctggcctgg ccgcgcctcg caggaccgag ctctcaaggt ctatctcagc
     4981 caggcgcaaa gccgcctccg ccgcccggac gtcggcgaga agctgcgggc gctcgctttc
     5041 gacgaggccg atccggtcgt tcgaacccgc cacggccgcc tccgccccgg ccacatcggc
     5101 cagcgctttc ctgctcgcgg cgaccgcctc gtcgccgcgt gctctcgaca cccatccgtc
     5161 ggcggacgag cacttcggcc acgaagcccg cgaccttggc gctgagcggc gtcacgtcgc
     5221 cccgcacata ggcattgtcg gtcccgttcg cccgcccgcc gccggccact cccaaagctg
     5281 cccccgcgaa cacgatgagc gcggccagca gcgccgcgac ggccgatctg gaaagggtca
     5341 tcgggcgctt ccggtcgcgg tacgggggga gccggccaag ggcggctggc gaggaaacag
     5401 cagcaggatc gccgcgctgg cgatgagcat cgcgccgacg agcaggaagc cgtcgttgaa
     5461 cgcgagcacc cacgcttcgc ggtgtagcgc gccggcgagg gtcgcgatcg tgcgggcggc
     5521 cgcgagcgcg tcgtcgccgg cccgcgccgc aaggctgccg gcgagggcat cgagccggtc
     5581 gaggttggat ccgtaggcgg cagcgctttc ggcgagcgcg gcgacgtgga atttctgccg
     5641 ctcggtcacc agctcggtcg ccagcgcaat gccgagattg gtccctcccg cggtggtgac
     5701 gttgaacatc aacgatgccg tcggcccgtc ggcaggcttc agcggcgagc cgccggcgac
     5761 gaggttcgga accaggaaga gcacctggcc ggctccggcc aaggcatagg aaatccggaa
     5821 ttgctcggcg gccgcaagcg aggtgctgcc cgaagccagc agcgccgcga gcccgaacag
     5881 gatcagtccc gccccgatga tcagccgtgc gtcgacccgg tgcagcagcc accatgcgat
     5941 cggaaaggcg agcagctgcg ggacggcggc ccagaggaac aggttcgcga gctccagcgg
     6001 ccggtacccc tgcaccatcg ccagcaactg gggcgccagg aaggcggtac tcgcgaagcc
     6061 cgcccggaac aggaggttga gcgtgatcgg ggcgatgaag ctcagcctgg cgagcagcgc
     6121 caggctcagc accggccgcg gcgagcgcca agccgtgacg aggaatcccg cccaggcgcc
     6181 ggcgccgatc gccagcgacc agcggatcaa gtcgctgtcg agccagtaac ggcgcgtgcc
     6241 ctggccgagc acgagcatgg tggcggcgag cgccaggcag agcaggagca aggccgccca
     6301 atcgcggcgg agcgcgtccc aggcgatcgg cgcgcgcgga atgaagagca aagcggccgc
     6361 cgccaggccc gacccgatcg ccgcctggac gaggaaaagc gcttcccacc cgacccggtc
     6421 ctcgatcagt ccggaggcgg ccgggccaag cgtcgtcggc agcaggagaa acagcgcgag
     6481 cagcgaaagg ccgaacggca ggcgcggccc ggcggcggtc atgaaggtcg cggcaaaggc
     6541 gatcggccca aagccgccgg ccgcggagcc ctgcagcagc cgaagcacga tcataacggg
     6601 cagcggaggc gcggccgcgc atcccagcgc cgcaaaggcg aagaccaacg cggccgccgc
     6661 caggcatcgg cgcagcccga agcttgcgat cagcggcgag gagacgacga tcccggcgag
     6721 aagcgccatg gtgtagcccg tcgtcagcca ggacgcttcg tccgcggagg ccccgatcgc
     6781 gccctggata tcggccaggt tcgaggtcac gaactggccg gagaagttta ccagcaacga
     6841 tcccgacaaa gcggtggcga gcagcagcac gctgtcccga ggcagcggct cgagcgctgc
     6901 ctggggcccg gcggggcgag gggcggcatc agccggcacg atcgatccgc tccccgccgc
     6961 gcgccgccgg ctagaccaat cggatcacgg cttcgatgtt gccgcgcgtc gccttcgaat
     7021 aagggcaggt ctgatcggcc tcttcgagca gttcctcggc cagcgcccgg tcgaccccag
     7081 ggatcgtggc gttgaggcgg gccgcgatcg aatagctgct gtcgcccttg cgcaggtcga
     7141 cttcggcatc gatggtgacg ccgtcgggga gcttgacgcg ccgcgcgcgg gcgacttcgg
     7201 cgaccgccgc ctcgaagcag gccgaccagc cggccgcgaa cagctgctcg ggattggtac
     7261 ccaccgcgtg cgtgccgggc gtggcgagcc ggacttcgag attgccatcg tcgctcctcg
     7321 actggccctg gcggccgccg atcgtgtgcg tccgcgccgt gtagattacc ttgtcgctct
     7381 gatccatgag atcctccttc agccgtggtg cggcgccagg tgcgcgccgg ggagcgaaat
     7441 ctccgccgac gcccatcttc cgtgcacggc aatgtcgccg aacaagcgtg gatgtgcgca
     7501 agcggcgggc gggccgccat tgtgccttgg tatgggctat acttgagtgg gtgccgggcc
     7561 gctgggctcc gcccagcaga ggctcgagcg ggtggattcc gaaacctagg ttcaatggtc
     7621 gccggcctgt cgccgagcta tgacgcggcc gtgcatccgg gttcctgatc gggagaaaat
     7681 ccatgcctca catcaatgtg caagtgacgc gcgaggggac cgcgcccggc gtcgatcgcg
     7741 tgaccccgga gcagaaggcc gccatttaca agggcatgag ccaggtgctg ctcgacgtgc
     7801 tcggcaagcc gctcgaccgg accgtcgtca tcttccagga agtcgagctc gaggattggg
     7861 gccgcggcgg catgccgatc gcggaatttc gcaagctggg cgcggcatcc ggcggctgac
     7921 gccggaccgg agcggtccgc cggcgccgac cctccggaat gatcgccggc gtgggaatgg
     7981 tctgggcgcc ccgaccttcg ggctcgcgaa ggacgccgac cgtcgctagg gacgcccccc
     8041 tcatgacagg gaggcgaata tggggagcga ggcagtagac gaggacggcc cgtttctgga
     8101 actcgccgac agcgtgccgg tcatgctgtg gcgcataaac tcgacctttg attgcgactg
     8161 ggcgaacaag gcatggttcg agtttaccgg cgggacgctg ccgcagcaga ccggctttgc
     8221 atggctggat cttgtccatc ccgacgacag cgaccgcgtt gtcgaggcgt tcgggcgcgc
     8281 cttcgaagcg cgggagaagg tctcggtcga gttccgcatc cgccgggtcg acggccgcta
     8341 ccgctgggtg ctggacagcg gctcgcctta tttccgcgat ggcctgttcg ccggcttcat
     8401 cggaagctgc gtcgacatca ccgaacgcaa ggaggcggag cagcgaacgg aggcgctcgc
     8461 cgcggaaatg gtccggctgt cccgggccga agccttgagc tcgcttgcaa ccgcgatcgt
     8521 tcacgatctc gcccagccgc tccaggcgat cggagcctat gcggaggggg tagcggaaat
     8581 tctcgccaat cgcggcccaa gctccgagga agcgatcgcc ggcgtcaacg aaatccgcga
     8641 agcggcggcg cgggccgcgg ggatcgtgcg gaactgccgc agccttgccg cagggcggaa
     8701 gagcgaacgg gtgcgcgccg acgtggcggc ggttctgcgc ggggccgagc cctggatccg
     8761 gtcccatccg gacgccgcgg gcgtgagcat cgactggaat ctggcgagcg gcctcgaggc
     8821 cgagttgagc ccgctggaga tcgagcagct gctgatcaac ctcgcctcca acgcgctcga
     8881 ggcgatgcgc ggcatgccgg gcgcggagct tcgcatagcg gccgcgcgct ggggcgggct
     8941 cgccatcgtc tcggtcgccg acaccgggcg cggggtgccg cccgacatgc gcaagatcat
     9001 cttccgatcg ctagtgacca ccaaaccgaa aggcatgggc atcggcctct acgtctgcca
     9061 cgccatcgcg accgcgcatg gcgggcgcat ctgggtcgag gctggagccg aaggcggcgc
     9121 cgtcttcaag ttcacgctgc cgctgacgcc ctgaccgcgc tggagccggc tgtgcgcgaa
     9181 cgaataatca gcatgggcgg aggtggagac gcgggcgacc gacgagaccg taatccgtgc
     9241 gcgagcgatg ctctacagtc ggtcacgccg ctgaggatag tgcgacttac ggtcgtttca
     9301 tcgtgtgaga ctgccacagt ggaggtggca tagacgtaaa cccgggcagc gcaacgctac
     9361 cagcagtgcc ggtgagacat tgcaatccct gcttggtccg gacaccctcc gtagcacctg
     9421 tcaatggggt cgtggaaaat ttgtcaacgg tggcattgac gcgtgacttg cgcgcgccaa
     9481 gcaactgctc cgcccgggtt gacaaacggt cgcgccgctg gccacttcgc caggggaggg
     9541 tcgaccgatg ctgcttcgcc ggtgggcagc cttgctatca ttgctgatcg catggtcgct
     9601 cgccgtccac atcccttctg catacgctgc cgaggcggag cgcatcgtgg cgatcggcga
     9661 ccttcatggc gaccacgccg cgtggcggca gatcgcggcg gcggcggggc tgattgatca
     9721 agcggggcgg tgggccggcg ggcgcgccat cctcgtgcag acgggcgatg tcaccgatcg
     9781 cggtcccgat tccctcaaga tcattcggga cttgcgccga ctgcagtccg aggcgcagcg
     9841 ggcgggcggg cgcgtgatcg cagtcgtcgg caaccacgaa gccatgaacg tcaccggcga
     9901 tctgcgctac gttcatcccg gcgaatatgc cgctttccgg gg
//
LOCUS       DASVRK010000059         4807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234 k141_9619,
            whole genome shotgun sequence.
ACCESSION   DASVRK010000059 DASVRK010000000
VERSION     DASVRK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9619"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..680
                     /locus_tag="VF589_02150"
     CDS             396..680
                     /locus_tag="VF589_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256411.1"
                     /translation="MRREFGRKIDWVTWTHKAAAEQRRFGFSEEVAQAEAVKARERLK
                     LRLAWVRERFALSSEMEDRAGKAYDAMFDAIRRSTGATPTRPSCPSRAKG"
     gene            818..1240
                     /locus_tag="VF589_02155"
     CDS             818..1240
                     /locus_tag="VF589_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256412.1"
                     /translation="MAAVRAAPVKRVSLEKDGVVVPAAPVEAVGREVAAEEESPEEAL
                     ATFLAKLEEIEARPEPCPEGWWVGPGGRVIPDGWGPVTEAAQAAWAEEQAAEEQAAAD
                     AAAGEEPERGPLWPGFQEEMSARWAARRAAEEGGARKF"
     gene            1421..1684
                     /locus_tag="VF589_02160"
     CDS             1421..1684
                     /locus_tag="VF589_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256413.1"
                     /translation="MATNVLEARGGSLIPKARGYHIVYREHQVNHCPGCGHTHWYIGR
                     VMAECAFCTTALPLETAPLVAGGQIVRTVRRSPMPEPEHAFAA"
     gene            complement(1828..2784)
                     /locus_tag="VF589_02165"
     CDS             complement(1828..2784)
                     /locus_tag="VF589_02165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8256414.1"
                     /translation="MRLTALAAAAGLAAAACTAPGAGAPDPEPSPRYTFWRPAPVEAA
                     PEPSETVAAAPAEAWRAVEPDNLLIMDLKDGSRVAIELAADFAPVHVANLRALARGGW
                     WDGAAIYRIQENYVAQWGQNESQRPLPAGVVKAPPAEYERALAGLPVRPLGFPDSYAP
                     IVGHVDSWPVGYDPERGFAWLAHCYGAVGVGRDLAPDTGTGGELYAVIGHAPRHLDRN
                     IAVVGRVIDNMPALTARPRGTEALGFIKDPKQHVPIARVRLAADMPAAERPYYEVLRS
                     GHEAFSAYVTGRANRGGDFFDIPAGGVDLCNVNVPVRRRPGS"
     gene            2888..3748
                     /locus_tag="VF589_02170"
     CDS             2888..3748
                     /locus_tag="VF589_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8256415.1"
                     /translation="MGILAGERAEWAGGGGGGRRATLLLIAGFWSAAFGLLSIRGALV
                     EPLPFATLGPRRMLLAIVGGCLCYGMARVMERLGQRPFGERMAWSLAGAIAMSALLTL
                     IHQVAYRVIDPLPGAPPICLRDGIVWGIVWLGYFAAWTGLHLALVAHFEAIGAGVPAP
                     AIAPVERPAPAPPLESFWAHRHREYVRVRIEDLVWAEAEGDYVRLHAAGGGGLVRETL
                     RAIEQALDPRLFIRVHRSALVRRSAIAALRRKESGAIVLALAGGGEVPVGRRYGKGLR
                     ALIGEMRREG"
     gene            3872..4336
                     /locus_tag="VF589_02175"
     CDS             3872..4336
                     /locus_tag="VF589_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8256416.1"
                     /translation="MPRERAPLLRFLLLPGLARHGDLGLLLLRIVTGGFLIHQSHDNI
                     VSAARMDEFVKFMTQFGFVYPELMAPLSVWWQTIAGIGFILGLGTRWLGFITMFQFII
                     ACWMVHWTLDVAGWWPALILVFLGLYFGLRGSGRYGIDAVLERRLGERGSRG"
     gene            complement(4399..4602)
                     /locus_tag="VF589_02180"
     CDS             complement(4399..4602)
                     /locus_tag="VF589_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256417.1"
                     /translation="MPLLFPKKRLSERELTVRAGEQLREHGVNAPARCAVTAERCLQQ
                     GRIEEANRWRAVSDRLREMLAAA"
BASE COUNT          689 a         1443 c         1914 g          761 t
ORIGIN      
        1 gctttgagcg gttctttatc aaacagcatc cgcgggttgt gcggattggg ccttagcgac
       61 tttgttggga cgcgatccgg ggttgattcc ggcgcaggtg agatggaatt gttcggtgca
      121 tcactcattt ggcattcccc agccgtgtgt acagacgtga accctgcaaa cgcaagcttg
      181 gtcttctcga ccgcctaggc tccccgctcc cgtggaggaa gaacggttgg cgacgttggc
      241 tgatcaggcg cccaacgctc atgttccgcc gaactccggc agaccgcgcg ggaacagaaa
      301 tagaactttc ctacactccc ccacctgtga tctaagctga tcggttagcc gcgactcggg
      361 aagggagcgg ggcggcggtg aggatggggt tgagcatgag gcgggagttt gggcggaaga
      421 tcgattgggt gacgtggacc cacaaggcgg cggcggagca gcgccggttc gggttcagcg
      481 aggaggtggc gcaggccgag gcggtgaagg cgcgggagcg gttaaagctg cggctggcct
      541 gggtgcgcga gcggtttgca ctgtcgagcg agatggagga ccgggccggc aaggcctatg
      601 acgcgatgtt cgacgccatc cggagatcga ctggggcgac cccgacgcgc ccgagctgcc
      661 cgagccgcgc gaaggggtga tcgccgacgc gatatccgag gaggtgatgg cggcggtgga
      721 ggaggatcgc tggccgcggc acctctattt ccatcatgtc taagccatcg tcgcgaagtg
      781 gtacttcgcg ccgggaaggc ggaggaaggg gcgcggcgtg gcggcggtcc gcgctgctcc
      841 ggtgaagcgg gtcagcctgg agaaggacgg ggtcgtggtg cccgccgctc cggtggaggc
      901 ggtgggacgg gaggtggcgg ccgaggagga aagcccggaa gaggcgctgg cgaccttcct
      961 cgccaagctg gaggagatcg aggcgcggcc ggagccgtgc cccgagggct ggtgggtcgg
     1021 ccccggcggg cgcgtcattc ccgacggctg ggggccggtg accgaggcgg cgcaggcggc
     1081 atgggcggag gagcaggcgg ccgaggagca ggcggcggcg gacgcggcgg cgggggagga
     1141 gcctgagcgg ggtcctttat ggccgggctt ccaggaggag atgagcgcgc gctgggcggc
     1201 gcggcgggcg gccgaggaag gcggcgcaag aaaattttaa gactgttgca tttccggctc
     1261 agcgctttgg gagaacgggc tgccgagggg tatttacatg gtttatggag cggcgggcgg
     1321 catccgaacg cgggcgcggc tgagcggttc ggggcaagcc tatgaaactt ctcgaactca
     1381 tcgctcgtta tgcccccaag tcgagtagaa ggagccatgt atggcgacga atgttctgga
     1441 agcgcggggc ggcagcctta tcccgaaagc ccgcggatat catatcgttt accgcgagca
     1501 tcaggtgaac cattgcccgg gttgcgggca cacccattgg tatatcgggc gggtgatggc
     1561 ggaatgcgcc ttctgcacca ccgcattgcc gctcgagacc gcgcctttgg tggccggtgg
     1621 gcagatcgtg cggaccgtgc gccgctcgcc gatgcccgag ccggagcatg ccttcgcggc
     1681 ctgagccggt gccgccgatg cgcggcccga atataggaga cagggcgggt gcgccgtagc
     1741 ggcaccgccc gaggaacgcc ggccccgata cgcgagggcc ggcgttgccg tgtgtggggt
     1801 gtgcgatagg ctcttcgtta aggcgtgtca gctgcccggg cggcggcgga cggggacgtt
     1861 gacgttgcac aggtcgaccc cgccggcggg gatgtcgaag aagtcgccgc cgcgattggc
     1921 gcggccggtc acgtaagccg agaaagcctc atggccggag cggagcacct catagtaagg
     1981 acgctcggcg gcgggcatgt cggcggcgag gcggacgcgg gcgatcggca cgtgctgctt
     2041 cggatccttg atgaagccga gcgcctcggt gccgcgcggg cgggcggtga gagccggcat
     2101 gttgtcgatg acgcggccga cgacggcgat gttgcggtcg aggtgacgcg gggcgtggcc
     2161 gatgacggcg taaagctcgc cgccggtgcc ggtgtcgggc gcgaggtcgc gtccgacgcc
     2221 gaccgcgccg tagcaatggg caagccaggc gaagccgcgc tcgggatcgt agccgaccgg
     2281 ccagctgtcg acatggccaa cgatcggcgc gtagctgtcg ggaaagccga gcgggcgcac
     2341 gggcagcccg gcaagcgcgc gctcatattc cgcgggcggc gccttgacga cgccggcggg
     2401 gagcgggcgc tggctctcat tctggcccca ttgggcgacg taattttcct ggattcggta
     2461 gatggcggcg ccgtcccacc agccgccgcg ggcgagcgcg cgcagattgg cgacgtggac
     2521 gggcgcgaaa tcggcggcga gctcgatcgc gacgcggctg ccgtccttca aatccatgat
     2581 caggagattg tcgggttcga ccgcgcgcca cgcctcggcg ggggcggcgg cgacggtctc
     2641 ggacggctct ggcgcggcct cgaccggggc cgggcgccag aaggtgtagc gcggcgacgg
     2701 ctcaggatcg ggcgcgcccg cgccgggcgc ggtgcaggcg gcggcggcga ggccggcggc
     2761 ggcggcaagt gcggtaaggc gcatcgtctc ccctttattg ttcggcggcg aaactggcgg
     2821 gaaagcggcg ccgccgcaag ggcgggcggc gcttgcgcgg ggcggagcgg ccgctaagct
     2881 tgcggcgatg gggatattgg cgggcgagcg ggcggaatgg gccgggggcg gtggcggcgg
     2941 caggcgcgcg acgctgctgc tgatcgccgg cttctggagc gcggcgttcg ggctgctgtc
     3001 gatccggggc gcgctggtcg agccgctgcc gttcgcgacg ctggggccgc ggcggatgct
     3061 gctcgccatc gtgggcggct gcctgtgcta cggcatggcg cgggtcatgg agcggctcgg
     3121 gcagcggccg ttcggggagc ggatggcgtg gtcgctggcc ggggcgatag ccatgtcggc
     3181 gctgctgacg ctgatccatc aggtcgccta tcgcgtgatc gacccgctcc cgggcgcgcc
     3241 gccgatttgc ctgcgcgacg gcatcgtctg ggggatcgtc tggctcggct atttcgccgc
     3301 ctggaccggg cttcacctgg cgctggtcgc gcatttcgaa gccatagggg cgggcgtgcc
     3361 ggcgccggcc attgcgcccg tcgagcggcc ggcgccggcg ccgccgctcg aaagcttctg
     3421 ggcgcaccgg catcgcgaat atgtgcgggt gcggatcgag gacctggtct gggccgaggc
     3481 cgagggcgat tatgtccggc tgcacgcggc cggcggcggc gggctagtgc gcgagacatt
     3541 gcgcgcgatc gagcaggcgc tcgacccgcg gctgttcatc cgcgtccacc gctcggcttt
     3601 ggtgcggcgg agcgcgatcg cggcgctcag gcgcaaggaa agcggcgcga tcgtgctggc
     3661 gctggccggc ggcggcgaag tgccggtcgg gcggcgctac ggcaaggggc tgcgggcgct
     3721 gatcggcgaa atgcgccgag agggctgagc gctcgcccac ggcgcgcgcg cgggacgttc
     3781 gccgcagcca ggcgacattc gacgcggccg gctatgcgcg cgggcggaag cccttagtgt
     3841 atcggcgaga caatacagcc gggagttttc catgccacgc gagcgcgccc ccctcctccg
     3901 cttcctgctg ctcccgggcc tcgcccgcca cggcgatctc ggcctgttgc tgctgcgcat
     3961 cgtcaccggc ggcttcctca tccaccagag ccacgacaat atcgtcagcg cggcgcggat
     4021 ggacgagttc gtcaagttca tgacccagtt cggcttcgtc tatccggagc tgatggcccc
     4081 gctgtcggta tggtggcaga ccatcgccgg gatcggcttc atcctcgggc tgggcacgcg
     4141 ctggcttggc ttcatcacca tgttccagtt catcatcgcc tgctggatgg tgcattggac
     4201 gcttgacgtg gcgggctggt ggccggcgct gatcctggtt ttcctcgggc tgtatttcgg
     4261 gctgcgcggg tccgggcgct acgggatcga tgcggtgctc gagcggaggc tgggggagcg
     4321 ggggtctcgc ggctagtccc gcatgcgcgg gagtctgggc ccccgccttc gcgggggaac
     4381 gcatcgagcg tgcgccgctc aggcggcggc gagcatttcg cggaggcggt cggagacggc
     4441 gcgccagcga ttggcttcct cgatccggcc ctgctggaga cagcgttcgg cggtgacggc
     4501 gcagcgggcg ggggcgttga cgccatgctc gcgcaattgc tcgccggcgc ggaccgtcag
     4561 ctcgcgttcg gacaggcgct tctttggaaa caacaaaggc aaagcctact ccatcctcgt
     4621 tcacggcatc aacgcaggcg ggcgcggttg gtgccttggc gcggcccggg cgcggttggg
     4681 gcgggccgga gaaaccgaaa agccccggct atactcatgc cggggctcca gggagagttt
     4741 acgctggcgg gagaacgact tgatccaggt taggttccgg gctgccgcac ccggatagcg
     4801 ggggccg
//
LOCUS       DASVRK010000060         4917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_570103, whole genome shotgun sequence.
ACCESSION   DASVRK010000060 DASVRK010000000
VERSION     DASVRK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570103"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3389)
                     /locus_tag="VF589_02185"
     CDS             complement(<1..3389)
                     /locus_tag="VF589_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256418.1"
                     /translation="MADFPAAVSVASLTAATGVRIIGVAMHDRSGFSVASAGDVNGDG
                     VNDVVIGAPVASFGVGDAYVIFGAAGGLGASIDLAALDGTNGFRIGGRLSNAQTGTSV
                     ASAGDVNGDGYDDLIIGAPVADPGGLTDAGESYVLFGSGAGFAASVELSALNGSNGFR
                     IPGLSANDYSGRSVASAGDVNGDGLADFIIGAHKGDNTAGESYVVFGSTAGFPASFNI
                     ATLNGSNGFCLVGEDSYDLAGTSVASAGDINGDGYDDIIVGAPLAELAGQPANHQSGT
                     SYVVFGSGAGFAAKLDLAALDGTAGFRIDGLDQGDFFAESVASAGDINGDGFDDFIFG
                     APRAGTGGVSYVVFGSGAAFGASIDLAALDGSNGFRIDGIDAGDASGYSVASAGDVNG
                     DGFGDIIVGANRAYSTPGVFTTGESYVIFGSGAGFGASFDLATLDGSNGFRIDGAEAN
                     DESGTSVAAAGDVNGDGFDDLIVGAPRAEPDGARYNAGETYIIYGRAPTAGVTRTGSN
                     ADQTIRGGDFDDVLAGGGGNDLIIGGGGADSMSGGTGDDTYEVDDIGDTVAESAGEGN
                     DRVESSVSFTIGAGIETLALVGSGQLDGTGNSEANLIIGNNAANILTGGGGNDVLRGL
                     AGGDDMRGEAGDDVYEVDEATDTVTENAAEGIDRVESSLGFTLAANFENLTLTGTAAI
                     NGTGNSGANVIIGNNAANTLNGAGGADVMEGGDGDDVYLVLGGGATVTETSSTGGTDR
                     VESYVSFTLGDNLENLTLASTGSAATATGNALDNLIVGNFQNNTLDGAAGADTMNGGG
                     GNDIYLVDEIGDIVGEAAGAGTDEVRSLVTYTLTANIEQLLLQGSAAIDGTGNALANI
                     LNGNSAANRLDGGAGDDDMQGFAGDDTYVVDSAEDSVLEDTGTGTDTVESSVSFILDA
                     DLENLTLTGTAAINGGGNAGANVIIGNDAANILNGAGGADVMDGKGGNDVYMVLSAGA
                     TVSETSDTGGTDRVESYVSFTLGDNIENLTLASTGTAVAATGNALDNVLIGNVQNNTL
                     DGAAGADTMDGGGGNDVYVVDDAGDVAAETTAGGGTDLVESSVNHILGNNIENLTLTG
                     TAANGRGNAGANQIEGNHAANVIDGAAGADTMTGAGGNDEY"
     gene            complement(3760..4827)
                     /locus_tag="VF589_02190"
     CDS             complement(3760..4827)
                     /locus_tag="VF589_02190"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX8256419.1"
                     /translation="MPSQANSYAAYTADTPFAPFSFERRDVGPRDVAIDILYCGVCHS
                     DLHTARAEWGGTLYPCVPGHEIVGRVVAVGGEVSKFEEGDLAGVGCMVGSCGHCPSCA
                     EGEEQYCETTGMVGTYNGRDDVLGGHTFGGYSDHIVVDQDFVLAVRHPEADLAAVAPL
                     LCAGITTYSPLRHWKVGPGQRVGVVGLGGLGHMGVKIAAAMGAEVIVFTTSPGKREDA
                     LRLGADDVIVSRDAGQMKAQRGTFDFILNTVAAPHDLDAFLVLLKRDGAMVLVGVPAS
                     PHPAPSVGNLVMRRRSLAGSLIGGVAETQEMLDFCNEHGLTADIETIPIQAIDTAYER
                     MLKSDVKYRFVIDMRSLDEAG"
BASE COUNT          753 a         1663 c         1642 g          859 t
ORIGIN      
        1 aaatattcgt cgttaccgcc ggcgccggtc atcgtgtcgg cgccggcggc gccgtcgatg
       61 acgttcgccg cgtgattgcc ttcgatctgg ttcgcgcctg cgttgcccct gccgttcgcg
      121 gcggtgccgg tcagcgtcag attctcgata ttgttgccga ggatatgatt gaccgagctt
      181 tcgacgaggt cggtgccgcc gccggccgtc gtctcggcgg cgacgtcgcc ggcatcgtcg
      241 acgacataga catcgttgcc gccgcccccg tccatcgtgt cggcgccggc ggcgccgtcg
      301 agcgtattgt tctgaacgtt gccgatcagg acattgtcga gcgcgttgcc ggtggcggcg
      361 acggcggtgc cggtgctggc cagcgtcaaa ttctcgatat tgtcgccgag cgtgaagctc
      421 acatagcttt cgacccggtc ggtgccgccg gtgtcgctcg tctccgagac ggtcgcgccg
      481 gcgctcagca ccatatagac gtcgttgccg cctttgccgt ccatcacgtc ggcgccgccg
      541 gccccgttca aaatattggc cgcatcgttg ccgatgatga cgttagcgcc ggcattgccg
      601 ccgccgttga tcgcggcggt cccggtcagc gtcaaattct cgaggtccgc gtccaggatg
      661 aagctgaccg agctttcgac cgtgtcggtg ccggttccgg tatcctccag gaccgaatcc
      721 tcggcgctgt cgacgacata ggtgtcgtcc ccggcgaagc cctgcatgtc atcgtcgccg
      781 gcgccgccgt cgagccggtt ggccgcgctg ttgccgttga ggatgttggc gagggcgttt
      841 ccggtgccgt cgatcgcggc cgacccctgg agcagcagct gctcgatatt ggcagtgagc
      901 gtgtaggtga cgagcgatcg cacctcgtcg gtgccggcgc cggccgcctc cccgacgata
      961 tcgccgatct cgtcgacgag atagatgtcg ttgccgccgc ctccgttcat cgtgtccgcg
     1021 ccggcggcgc cgtcgagcgt gttgttctgg aaattgccga cgatgaggtt gtcgagcgcg
     1081 ttgccggtgg ccgtggcggc gctgccggtg ctcgcgagcg tcaaattctc gagattgtcg
     1141 ccgagggtga agctcacata gctttcgacg cggtcggtgc cgccggtgct gctggtttcg
     1201 gtcacggtgg cgccgccgcc gagcaccaga tagacgtcgt cgccgtcgcc gccctccatc
     1261 acgtcggcgc cgccggcgcc gttgagggtg ttggccgcat tgttgccgat gatgacgttc
     1321 gcgcccgaat tgccggtgcc gttgatcgca gcggtcccgg tgagggtgag gttctcgaag
     1381 ttcgcggcga gggtgaagcc gagcgagctt tccacccggt cgatcccttc ggcggcattc
     1441 tcggtgacgg tgtccgtggc ttcgtccact tcgtagacgt cgtcgccggc ctcgccgcgc
     1501 atgtcgtcgc cgccggcaag gcctctgagc acatcgttgc cgccgccgcc ggtgaggatg
     1561 ttggccgcgt tgttcccgat gatgagattg gcttcggaat tgcccgtgcc gtccagttgg
     1621 ccgctgccga ccagcgccag cgtctcgatg ccggcgccga tcgtgaacga cactgagctt
     1681 tcgacccggt cgttcccttc gcccgcgctt tcggcgacgg tgtcgccgat gtcgtccact
     1741 tcgtaggtat cgtcgccggt acctccgctc atggaatcgg cgccgccgcc gccgatgatg
     1801 aggtcgttgc cgccgccgcc cgccagcacg tcgtcgaaat cgccgccgcg gatcgtctga
     1861 tcggcgttcg atccggtccg cgtgactccc gcggtgggcg cccggccgta gatgatataa
     1921 gtctctccgg cattgtacct ggcgccgtcc ggttcggcgc gcggcgcgcc gacgatcagg
     1981 tcgtcgaagc cgtcgccgtt gacgtcgccc gccgccgcca ccgaagtgcc gctttcatca
     2041 ttcgcttcgg cgccgtcgat gcggaagccg ttcgatccgt cgagagtcgc aaggtcgaag
     2101 ctcgctccga aaccggcgcc cgaaccgaag atgacgtagc tctcgcccgt cgtgaaaacc
     2161 cccggggtgg aataggcccg gttggcgccg acgatgatgt cgccgaagcc gtcgccattg
     2221 acgtcacccg cggacgcgac cgaatagccg ctggcgtcgc cggcatcgat cccgtcgatg
     2281 cggaacccgt tggagccatc gagagcggcg agatcgatgc tggccccgaa agccgccccg
     2341 gagccgaaca cgacgtagct cacgccgccg gtaccggccc gcggagcacc gaagatgaag
     2401 tcgtcgaaac cgtcgccgtt gatatcgcct gccgaagcga ccgactcggc gaagaagtcg
     2461 ccttgatcga gcccgtctat gcggaagccg gccgtcccat ccagcgccgc gagatccagc
     2521 ttcgcggcga agcccgcgcc ggagccgaac acaacgtagc tcgtaccgct ctggtgattg
     2581 gccggctggc cggcgagttc cgcgagcggc gcgccaacga tgatatcgtc atagccgtcg
     2641 ccattgatat cgcctgccga ggcgacggat gtgccggcca aatcgtagct gtcctcgccg
     2701 accaggcaaa agccgttgga gccgttcagg gtggcgatgt tgaagctggc ggggaagccc
     2761 gcggtcgagc cgaacacgac atagctttcg ccggcggtat tatcgccttt gtgcgcgccg
     2821 atgatgaagt cggccagccc atcgccgttg acgtcgccgg ccgacgcgac cgaacggccg
     2881 ctataatcgt tcgccgacaa gcccgggatg cggaagccgt tcgatccgtt gagagcgctc
     2941 agttcgacgc tcgcggcgaa tcccgcgccg gagccgaaca ggacgtagct ttcgccggca
     3001 tcggtgaggc cgcccggatc ggcaaccggc gcgccgatga tgagatcgtc atagccgtcg
     3061 ccattgacgt cgccggccga ggcaaccgac gtgccggtct gcgcattgct gaggcggccg
     3121 ccgatccgaa acccgttggt gccgtcgaga gcggcaaggt cgatgcttgc gcccaaaccg
     3181 ccggccgcgc cgaaaatcac ataagcatcg ccgaccccga aggaagcgac gggcgcgccg
     3241 atgacgacgt cgttgacgcc gtcgccgttg acatcgcccg ccgaggcgac ggagaagccg
     3301 ctccggtcat gcatggcgac gccgatgatc cggacaccgg tcgcggccgt gaggctcgcg
     3361 accgacacgg cagcggggaa atcagccatt cagcccctcc tgactggcat tgaggggccg
     3421 ccggcgcacg ctcgccgcgc tcagcgcgcg cgcagcggat tgcgctttga gtcgcggcat
     3481 ggcccctgcc ccccaatttt tcgcgggtcc gtctgctacc ccggtgcgac tcgacggaaa
     3541 acgctacgtc tctaaggcgt tagacctgcg cttcgcggcc cgtcaatgtc aagtcgagcc
     3601 aaagtctttg ccaagacgag cggcagtcgc gctccgcgcc cggcgaacag aagcccgcca
     3661 gctggaaaat cactctccgc tgcccgtctt tggccggccc ggcgcgactt gcccgtcata
     3721 tcggcacttc ggcggcctcg attccggcca agaccccgct cagccggcct catcgagcga
     3781 gcgcatgtcg atcacgaaac gatatttgac gtcgctcttg agcatccgct cataagcggt
     3841 gtcgatcgcc tggatcggga tcgtctcgat atcggcggta agaccgtgct cgttgcagaa
     3901 atcgagcatc tcctgggtct cggcgacgcc gccgatcagc gagccggcca gcgaacgccg
     3961 ccgcatcacc agatttccga ctgagggcgc gggatgcggc gaggccggga cgccgaccag
     4021 caccatcgct ccgtcgcgct tcaggaggac caggaaggcg tcgaggtcgt ggggggcggc
     4081 gacggtgttg aggatgaagt cgaacgtccc gcgctgcgcc ttcatctgcc cggcgtcgcg
     4141 ggaaacgatc acgtcgtcgg cgccgagccg gagcgcgtcc tcgcgcttgc ccggggaggt
     4201 cgtgaagacg atcacctcgg cgcccatcgc ggcggcgatc ttgaccccca tatggccgag
     4261 cccgccgagc ccgaccacgc cgaccctctg gcccggcccg accttccagt gccgcagcgg
     4321 cgaataggtc gtgatgcccg cgcacagcag aggcgccacc gcggcgagat ccgcttccgg
     4381 gtggcgcacg gccagcacga aatcctggtc gacgacgata tggtcggaat agccgccgaa
     4441 cgtatggccg ccgagcacgt cgtcgcggcc gttataggtg ccgaccatgc cggtggtctc
     4501 gcaatattgc tcctcgccct cggcgcagga cgggcagtgg ccgcagcttc caaccatgca
     4561 gccgacgccg gcgaggtcgc cttcctcgaa cttgctgacc tcgccgccga ccgccacgac
     4621 gcgcccgacg atctcgtggc ccggcacgca agggtagagc gtgccgcccc attcggcgcg
     4681 ggcggtatgg aggtcggaat ggcagacgcc gcaataaagg atgtcgatcg cgacgtcgcg
     4741 cgggccgacg tcgcgacgct cgaagctgaa cggcgcgaac ggcgtgtcgg cggtataagc
     4801 ggcgtaggaa ttggcttggc tgggcatcgg ttacctctgg gaagtgatcg gcgggacttg
     4861 aatggacagg gggcccgggc gtcgcccgcg gcctcgcctg ccctaacgcg ccgacgc
//
LOCUS       DASVRK010000061         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_383468, whole genome shotgun sequence.
ACCESSION   DASVRK010000061 DASVRK010000000
VERSION     DASVRK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383468"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1045)
                     /locus_tag="VF589_02195"
     CDS             complement(98..1045)
                     /locus_tag="VF589_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEX8256420.1"
                     /translation="MITIQYVFYLVGALFGAWALLSAFDRTNPKRFGNAVFWGLMALS
                     LIAGHRLGDVTNGFLVLGLAALATLKLLGRGAPPAREAEKAAAGERYGNALFLPILIV
                     LATAVVGTLLYLYTPLNRSGLFEANRETFILLCLGVLLALAVIFVWLKPPPLAPLDEG
                     RRLIDAVGWAAVLPLMLAALGLVLADTGVGKTIGALMLTVIPEGTVLPAVIVFGLGMA
                     LFTILTGNAFAAFPVMATAIGVPLLIQTYGGDPAVVGAIGMLAGFCGTLMTPMAANFN
                     LVPAALLELKDQNGVIKAQIGTAIPLLIVNILLIYFLAF"
     gene            complement(1042..1734)
                     /locus_tag="VF589_02200"
     CDS             complement(1042..1734)
                     /locus_tag="VF589_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEX8256421.1"
                     /translation="MNYLPLLGILVVILGFALRFNPMLVVPAAALTTGLLAGFDLVEV
                     IGKLGKAFNDARIIIIPYVVLPVIGLLERYGLQQRARAVISSFRAATAGRLLILYLLY
                     RQFISAIGLHSTAGHPQTVRPLVAPMAVAAAEQQGALDEATTDEVKAYAAATDNVGLF
                     FGEDIFFAIGSIVLIQQTLEAYGIILSPLELAVWAIPSAIAAFLIHGSRLLMLDRRLG
                     KPSPRNRGEDLP"
     gene            complement(1884..>3642)
                     /locus_tag="VF589_02205"
     CDS             complement(1884..>3642)
                     /locus_tag="VF589_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX8256422.1"
                     /translation="PHAGHGEALDRASLCTGASVQGPALIIDPSATTVVEPGWQAEVD
                     RLGNLILTRSVPREAARAVGTAADPIMLEIFNNLFMAIAEEMGVALQNTASSVNIKER
                     LDFSCAIFDQGGALIANAPHIPVHLGSMGDSIRTIIDARGAGSDGHGRDGRGMKPGDA
                     YVLNAPYRGGTHLPDITVIMPVFIEGEASPAWYVAARGHHADIGGTTPGSMPPDSKTV
                     AEEGVLIDNQLLVDAGAFREAEIRALLASGAWPARNPNVNIADLKAQVASCTRGANEL
                     RRVAGEYGREAIDAYMGHVMANAEEAVRRLISGLRDGSFTYKMDNGAHVSVAVTVDPA
                     SRSAVIDFAGTSGQRPDNFNAPYSICRAATLYVFRTLVDDAIPMNDGCLRPIELRVPE
                     GSMLNPLYPAAVVAGNVETSQVVTDALFAACGALAPSQGTMNNFTFGSDRYQYYETIA
                     GGAGAGPDFDGASAVQTHMTNSRLTDPEILETRYPVLLERFAIRRGSGGAGRHKGGDG
                     VVRHIRFRERMRANILSNRREVAPAGLAGGEDAEAGVNQVIRADGTVETLTATASAEM
                     APGDMFVIETPGGGGYGAQ"
BASE COUNT          607 a         1144 c         1348 g          543 t
ORIGIN      
        1 catggtggag ggggccccga gacccgccgc gcggatggaa agccccctcc accacgccgc
       61 ttcgcgtcgc ggtccccctc cccgttccgg ggaggagcta gaacgccagg aagtagatga
      121 gcaggatgtt gacgatcagc agcgggatgg cggtgccgat ctgcgccttg atgacgccgt
      181 tctgatcctt gagctccagc aaggccgccg gcacgagatt gaagttggcc gccatcggcg
      241 tcatcagggt cccgcagaag ccggcgagca tgccgatcgc gccgaccacg gccgggtcgc
      301 cgccataggt ctggatcagg agcgggacgc cgatcgccgt cgccatcacc gggaacgccg
      361 cgaaggcgtt gccggtgaga atggtgaaca gggccatgcc gaggccgaac acgatcacgg
      421 ccggcagcac cgtgccttcc ggtatcacgg tcaacatcaa cgccccgatc gtcttgccga
      481 cgccggtgtc ggcgagcacc aggccgagcg ccgccagcat cagcggcagc accgccgccc
      541 agccgactgc atcgatcagc cgccgcccct cgtcgagcgg cgccagcggc ggcggcttca
      601 gccacacgaa gatcacggcc agcgcgagca gcacgccgag gcagagcagg atgaaggtct
      661 cgcgattggc ttcgaacagg cccgaccgat tgagcggcgt gtagaggtag agcagggtgc
      721 cgaccaccgc ggtggcaagc acgatcagga tcggcaggaa cagggcgttg ccatagcgct
      781 cgcccgccgc cgccttttcc gcctccctcg ccgggggggc gccgcggccg agcagcttga
      841 gggtcgccag ggccgcgagg ccgagcacga ggaagccgtt ggtgacgtcg ccgagccggt
      901 gcccggcaat caggctgagc gccatcaggc cccagaagac ggcattgccg aagcgcttgg
      961 ggttggttcg gtcgaaggcg ctgagcaatg cccaggcgcc gaacagggcg ccgaccaggt
     1021 agaagacgta ctgaatggtg atcatggaag gtcctcccct cgatttcgag gggagggttt
     1081 tccaagcctg cgatccagca tcagcaggcg ggagccgtgg atgaggaagg cggcaatcgc
     1141 gcttgggatc gcccagacgg cgagctcgag cggggagagg atgatgccgt aagcttccag
     1201 cgtctgctgg atgagcacga tcgagccgat cgcgaagaag atgtcctcgc cgaagaacag
     1261 gccgacattg tcggtggcgg cggcataggc cttgacctcg tcggtggtgg cctcgtcgag
     1321 cgcgccctgc tgctcggcgg cggcgaccgc catcggcgcg accagcgggc ggacggtctg
     1381 cggatggccg gcggtggaat gaaggccgat ggcggaaatg aactggcggt agagcagata
     1441 gaggatgagc agccgccccg ccgtcgccgc cctgaagctg gagatcaccg cccgggcgcg
     1501 ctgctggagg ccgtagcgtt cgagcaggcc gatgaccggc aggacgacat aagggatgat
     1561 gatgatgcgc gcgtcgttga acgccttgcc gagcttgccg atcacttcga ccaggtcgaa
     1621 gccggcgagc aggccggtgg tgagggcggc ggccggcacg accagcatcg gattgaaccg
     1681 tagcgcgaag ccgaggatga cgaccaggat gccgagcaac gggagatagt tcacttcaaa
     1741 tcctcccctc gatttcgagg ggagggggac cgcgacgcga agcggcgtgg tggaggggcc
     1801 gccgcgaaga cggcggactg gcgtccgccg cccctccacc atgcgtcgca tggtccccct
     1861 cccctgcaaa tgcaggggag gatctattgc gcgccatatc cgcctcctcc cggggtctcg
     1921 atgacgaaca tgtcgccggg ggccatttcg gccgacgcgg tggcggtgag ggtttcgacg
     1981 gtgccgtcgg cgcggatcac ctggttgacg cccgcctcgg catcttcgcc gccggcgagg
     2041 cccgcgggcg caacctcgcg gcggttggac aggatgttgg cgcgcatgcg ctcgcggaag
     2101 cggatgtggc ggacgacgcc gtcgccgccc ttgtgccgcc cggcgccgcc cgagccgcga
     2161 cggatcgcga accgttcgag cagcaccgga tagcgggtct cgaggatttc ggggtcggtg
     2221 aggcggctgt tggtcatatg ggtctggacc gcgctggcgc cgtcgaaatc ggggcccgcc
     2281 cccgcgccgc cggcaatcgt ctcataatat tggtagcggt ccgagccgaa ggtgaaattg
     2341 ttcatcgtgc cttggctcgg cgcgagcgcg ccgcaggcgg cgaacagggc gtcggtgacg
     2401 acctggctgg tctcgacatt gccggcgacg acggcggccg ggtaaagcgg gttgagcatc
     2461 gagccttcgg ggacgcgcag ctcgatcggg cggaggcagc cgtcgttcat cgggatggcg
     2521 tcgtcgacca gggtgcggaa gacgtagagg gtggcggcgc ggcagatcga ataaggggcg
     2581 ttgaaattgt cggggcgctg gccgctggtg ccggcgaagt cgatcaccgc cgagcggctg
     2641 gccggatcga cggtgacggc gaccgagaca tgggcgccat tgtccatctt gtaggtgaag
     2701 ctgccgtccc tgagccccga aatcaggcgg cgcaccgcct cctcggcatt ggccatgacg
     2761 tggcccatat aggcgtcgat cgcctcgcgg ccatattcgc cggcgacgcg gcgaagctcg
     2821 ttggcgccgc gggtgcagga ggcgacctgc gccttcaagt ccgcgatgtt gacgttggga
     2881 ttgcgcgccg gccacgcgcc cgaggcgagc agggcgcgga tctcggcctc gcgaaaggcg
     2941 ccggcgtcga ccagcagctg gttgtcgatc agcacgcctt cctcggcgac cgtcttgctg
     3001 tccggcggca tcgagccggg ggtggtgccg ccgatatcgg cgtgatggcc gcgggcggcg
     3061 acataccagg ccggcgatgc ctcgccttcg ataaacaccg gcatgatcac ggtgatgtcc
     3121 ggcaggtggg tgccgccccg atagggggcg ttcaggacat aggcgtcgcc cggcttcatg
     3181 ccgcgcccgt ccctgccatg gccgtcgctg ccggcgccgc gggcgtcgat gatggtgcgg
     3241 atgctgtcgc ccatcgagcc gaggtggacc gggatatgcg gggcgttagc gatgagggcg
     3301 ccgccctggt cgaagatcgc gcaggagaaa tcgagccgct ccttgatgtt gaccgacgag
     3361 gcggtgttct ggagcgcgac gcccatctcc tcggcgatcg ccatgaagag gttgttgaag
     3421 atctcgagca tgatcggatc ggcggccgtg ccgaccgcac gcgctgcttc gcgcgggacg
     3481 gagcgggtga ggatgaggtt gccgagcctg tccacctcgg cctgccagcc gggttcgacg
     3541 actgtggtgg cggagggatc gatgatcagg gcgggtccct gaaccgacgc tccggtgcac
     3601 aaggacgcgc ggtccagtgc ctccccatgc cccgcatggg ga
//
LOCUS       DASVRK010000062         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_197118, whole genome shotgun sequence.
ACCESSION   DASVRK010000062 DASVRK010000000
VERSION     DASVRK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197118"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2296
                     /locus_tag="VF589_02210"
     CDS             <1..2296
                     /locus_tag="VF589_02210"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEX8256423.1"
                     /translation="ASVGDYRTARAGLAGRYAVAPKAGTATLVGEVSANQVAGGGALK
                     PIAAALAGAGGTPFEPIGDALAAAVRRVGESFDARGALRFVTGPGFSAARLQRLRAQS
                     RSGALFQLEGETGLTYYWPSGTTRIQGDVALSGGGFPDMRFALDQARPGGAISGAGRI
                     APMAAGGARLALGEVRFNAAPGGATSLNTIAVMDGPFAGGRVTGLVVPVSGRLDGDGG
                     LVFGERCTPVSFGQLQTGGVDLGPTRLPLCPSGPGLVWRRPGGALMAAAEVRAPRLAG
                     RIGGTPLAVTGDRLRFELAGRTFAGTSVAIGLGQGQANRFDFASLTGRFVRGGAAGDF
                     AGGDAKLANVPLLISEGGGKWSLLGGDLALSGAVRVADEAQPERFYPLVSKDFSLTLK
                     DGRIAANGLLTHPETGTRVTEVAIDHRLATGTGRAVLDVPGITFTEDFQPEALTRLTT
                     GVVALVAGTVTGRGEIAWGPDGTTSTGSFSTRGMNLAATFGPVEGLTTTVNFTDLLGL
                     ATAPGQTAQIGVVRTGIDVFDGLIRYQLLPGLRVRVEAGRWPFAGGELLLEETILDFS
                     RPSPKRLTFRVAGLDGAIFVQQMEFSNIAATGIFDGVIPMVFDEGGGRIVAGRMEARP
                     QGGELSYIGELTDRQLGYYGKVAFDALKSMRYQKLNIDLDGSLDGEFLAAIELDGIAR
                     NTPRPGGIAGAVFTQLAKIPFEFNIRVRGPFRALLATARSLEDPTLLIQSVLPEILQD
                     RPTTTTDVQPTTTNVQPQESETVQ"
     gene            2392..2556
                     /locus_tag="VF589_02215"
     CDS             2392..2556
                     /locus_tag="VF589_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEX8256424.1"
                     /translation="MIAAAGLATLAGCVQVSAPEKPIEINLNINIRQEVVVRLQEDVR
                     ELIEKNPGVF"
     gene            2559..2945
                     /locus_tag="VF589_02220"
     CDS             2559..2945
                     /locus_tag="VF589_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEX8256425.1"
                     /translation="MMRRSQLIFAAAAALSIMGGAGHAAAQADASAALRAGGKAGEQA
                     DGYLGVVGSADAATRAQVDAVNIKRRAFYTDLAAKRGAKIEEVAATTACQILASRVQP
                     GQYYRVGGEWRKRDAGAAVQRPDYCT"
     gene            complement(3075..3464)
                     /locus_tag="VF589_02225"
     CDS             complement(3075..3464)
                     /locus_tag="VF589_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8256426.1"
                     /translation="MSFSQRKAGPEGRLRAGVGHAGERTRQGAVPLGRGARRVNSARA
                     LRRAVTRPARRDRQGTGKRTTARQPARDEARDEWLMAQGYRTLRIPAREILNNLDGAL
                     GYILAQCQPLHPPPAAGGPPPRSGEEF"
     gene            3463..3783
                     /locus_tag="VF589_02230"
     CDS             3463..3783
                     /locus_tag="VF589_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX8256427.1"
                     /translation="MAEDEPGQDPKLPEDARLASLDERLRQAQHAEAARTGQARKSAD
                     RSEQLGNRVLSYLIGGLAGGALIGWVLDRWLGTSPLLLISLLILGTVGGFWNIIKIST
                     QRPD"
     gene            3822..>4192
                     /locus_tag="VF589_02235"
     CDS             3822..>4192
                     /locus_tag="VF589_02235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX8256428.1"
                     /translation="MAAEEGKIDPMHQFEVQSIFEGFSLGGHQIAFTNSALWMLITLG
                     ALWLFMLGGMKRQLVPTRWQVAVEGVTGFITSMMNTNIGPEGRKFTPYVFSLFMFILV
                     ANLIGMLPTGIVGIHPFTVTS"
BASE COUNT          616 a         1495 c         1435 g          646 t
ORIGIN      
        1 cgcttcggtc ggcgactatc gcaccgcgcg cgcggggctg gccggccgct atgcggttgc
       61 gcccaaggcg gggactgcaa ctctggtcgg cgaggtatcg gctaaccagg tcgccggggg
      121 cggcgcgctg aagccgatcg cggcggcgct ggcgggggcg gggggcactc cgttcgaacc
      181 gatcggcgac gcacttgccg ccgccgtgcg acgggtaggc gagagcttcg acgcgcgcgg
      241 cgcgctgagg ttcgtcaccg gccccggttt ttccgccgcc cggctccaac ggctgcgggc
      301 gcaaagccgg agcggcgccc tcttccagct cgagggcgag accggcctca cttattattg
      361 gccgagcggg accacccgca tccagggcga cgtcgcgctg agcggcggcg gcttccccga
      421 catgcgcttc gcgctcgacc aggcacggcc cggcggcgcg atcagcggcg ccggccggat
      481 cgcgccgatg gcggcgggcg gcgcgcggct ggcgctcggc gaggtccgct tcaacgccgc
      541 gccgggcggg gcgaccagtc tcaacacgat tgccgtcatg gacgggccgt tcgcgggcgg
      601 ccgcgtcacc ggcctggtgg tgccggtcag cgggcgcctc gacggcgatg gcggcctggt
      661 gttcggcgaa cgctgcaccc cggtgagttt cggccagctc cagacgggcg gcgtcgacct
      721 cgggccgacg cggctgcctt tatgcccatc tgggccgggc ctggtgtggc gcaggcccgg
      781 cggcgcgctg atggctgcgg ccgaagtgcg cgcgccccgc ctcgccggtc gaatcggcgg
      841 cactccgctc gccgtcaccg gcgaccggct gcgcttcgag cttgccgggc ggaccttcgc
      901 cggcaccagc gtcgccatcg gtctcggcca aggccaggcc aatcgcttcg atttcgccag
      961 cctcaccggc cgcttcgtcc ggggcggcgc ggccggcgac ttcgccggcg gcgacgcgaa
     1021 gctcgccaac gtgccgctgc tgatcagcga aggcggcggc aaatggagcc tgctcggcgg
     1081 cgacctcgcc ttgtcggggg cggtgcgggt cgccgacgag gcgcagccgg aacgcttcta
     1141 tccgctggtc agcaaggatt tcagcctgac gctcaaggac ggccggatcg ccgccaacgg
     1201 cctgctcacc catcccgaga cggggacgcg ggtcaccgag gtcgccatcg accacaggct
     1261 ggcgaccggc accggccggg cggtgctcga cgtccccggc atcaccttca ccgaggattt
     1321 ccagcccgag gcgctgaccc ggctcaccac cggcgtcgtc gcgctggttg ccggcaccgt
     1381 caccggccgc ggcgagatcg cctggggacc ggatgggacg accagcaccg gcagcttctc
     1441 gacccggggc atgaacctcg ccgccacttt cggcccggtc gaggggctca ccaccacggt
     1501 caattttacc gacctcctcg gcctcgccac cgcgcccggc cagaccgccc agattggcgt
     1561 ggtccgcacc ggcatcgacg tgttcgacgg gctcatccgc taccagctgc tgccgggcct
     1621 gcgcgtccgg gtcgaggccg ggcgctggcc gttcgccggc ggcgagctgc tgctcgagga
     1681 gacgatcctc gatttcagcc gcccgagccc caagcggctc accttccgcg tcgccggcct
     1741 cgacggcgcg atcttcgtcc agcagatgga gttcagcaac atcgccgcca ccggcatctt
     1801 cgacggggtg atcccgatgg tgttcgacga gggcggcggc cgcatcgtcg ctggccggat
     1861 ggaagcgcgg ccgcaaggcg gcgagctctc ctatatcggc gagctcaccg accgccagct
     1921 cggctattat ggcaaggtcg ccttcgacgc gctgaaatcg atgcgctacc agaagctcaa
     1981 catcgacctc gacggctcgc tcgacggcga attcctcgcc gccatcgagc tcgacggcat
     2041 cgcccgcaac acgccgcggc cgggcggcat cgctggggcg gtgttcaccc agctggccaa
     2101 gatcccgttc gagttcaaca tcagggtgag ggggccgttc cgggcgctgc tcgccaccgc
     2161 ccgttcgctc gaggacccga ccctgctcat ccagtcggtg ctgcccgaga tattgcagga
     2221 ccggcccacc accaccaccg atgttcagcc caccaccacc aacgttcagc ctcaggaaag
     2281 cgagaccgtg caatgaccgc atcgatagtg acgagagtgc gtctcgaacg ggcgcgggcg
     2341 ggaagccggc gatggggcgg cggccggggc ggcggcggcc gggcgaagga cctgatcgcc
     2401 gcggccgggc tggcgacgct cgccgggtgc gtgcaggtga gcgcgcccga aaagccgatc
     2461 gagatcaacc tcaacatcaa tatcaggcaa gaggttgtcg tgcgccttca ggaggatgta
     2521 cgcgagctca tcgagaagaa tccgggagtg ttctaagcat gatgcgccgt tcgcaactga
     2581 tcttcgccgc cgccgccgcg ctctccatta tgggcggcgc tggccatgcg gccgcgcagg
     2641 ccgacgcttc ggcggcgctc cgcgcgggcg gcaaggccgg cgagcaggcc gacggctatc
     2701 tcggcgtggt cggctcggcc gacgccgcga cccgcgcgca ggtcgacgcc gtcaacatca
     2761 agcgccgcgc cttttacacc gatcttgccg ccaagcgcgg cgccaagatc gaggaagtcg
     2821 ccgccaccac cgcctgccag atcctcgcca gccgggtgca gccgggccaa tattaccgcg
     2881 tcggcggcga gtggcggaag cgcgacgccg gcgccgcggt ccagcggccg gattattgca
     2941 cctgatctct gtcgatctcg atcgcgagcc tcgcggaccg gcagacgagt tcctctcagc
     3001 atcgtgccag cccctccacc gtgctgcgca cggtccccct ccccgtgccg gggaggattt
     3061 caggaggttg cggctcagaa ttcctccccg gaacggggag ggggaccgcc cgccgcaggc
     3121 ggggggtgga ggggctggca ctgcgccaga atgtagccca gcgccccgtc tagattattg
     3181 aggatttcgc gcgcgggtat ccgcagcgtg cgatagccct gcgccatcag ccattcgtcg
     3241 cgagcctcgt cacgggcggg ttgccgcgcg gtcgtgcgct tccccgttcc ttgccggtct
     3301 cgccgggccg gcctcgtcac cgcccggcgc aaagccctcg cgctgttgac tcgcctcgcc
     3361 ccccttccta aaggaaccgc gccttggcgg gttcgttcgc cggcatgtcc cacgcccgct
     3421 cgaagccttc cctccggacc ggcttttctt tgggagaaag acatggcgga agacgagccc
     3481 gggcaggacc ctaagcttcc cgaagatgcg cggctggcat cgctcgacga gcggctccgc
     3541 caggcgcaac atgcggaagc ggcgaggacc ggccaggcgc ggaaatcggc cgaccgcagc
     3601 gagcagctcg gcaaccgcgt cctgtcctat ctgatcgggg gccttgccgg cggagcgctc
     3661 atcggctggg tcctggaccg ctggctgggc acctcgcccc tgctcctgat ctcgctgctg
     3721 atcctcggaa cggtcggcgg gttctggaac atcatcaaga tctccaccca gcgcccggac
     3781 tgatcttcgg gcgtttggaa catttgaacg ggaacagacg cgtggcggcc gaagagggca
     3841 agatcgaccc gatgcaccag ttcgaggtgc agagcatctt cgaagggttc agccttggcg
     3901 gtcaccagat cgccttcacc aactccgctt tgtggatgct gatcaccttg ggagctttgt
     3961 ggctgttcat gctcggcggc atgaagcgcc agctggtgcc gacccgctgg caggtcgcgg
     4021 tcgaaggcgt gaccggcttc atcacctcga tgatgaacac caatatcggc cccgaaggcc
     4081 gcaagttcac gccctacgtc ttctcattgt tcatgttcat cctggtcgcg aacctgatcg
     4141 gcatgctgcc gaccggcatc gtcggcatcc acccgttcac cgtcaccagc ca
//
LOCUS       DASVRK010000063         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_135554, whole genome shotgun sequence.
ACCESSION   DASVRK010000063 DASVRK010000000
VERSION     DASVRK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135554"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1593)
                     /locus_tag="VF589_02240"
     CDS             complement(214..1593)
                     /locus_tag="VF589_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8256429.1"
                     /translation="MAWLIAAALGSAAALPAAAAPAAAPAAEAAKAKEILKRSVAFKT
                     VEGQGQVPAYAAFLASVLKEAGFPDSDIEIAKMGETATLVAHYRGTSDAKPIVLLGHM
                     DVVEAKPADWERDPFTAVEENGYIFGRGSEDNKFDVSMMVATLARLKAGGYKPRRTLI
                     LALSGDEETQMATTRALAKKFAGAELALNGDGGGGQLTEANAPIFYGLQAGEKTYADY
                     QLEVTDPGGHSSAPTGSNAIYRLARALGRLGDHAFPPMANALTTASLKTASRQVGGEL
                     GAAMARYAANPEDAAAAALISSRPEYVGQIRTTCVATMANAGHALNALPQRATANVNC
                     RIFPGISIDAVKQELARVVADPGVKISVIDDPAFSDASPLRPDVMQAVTKAVKARYPK
                     LEITPSMSAGATDSLHFRAAGVPSYGVSGLFMRAEDGFAHGLNERIPVAEIAPALAHW
                     RSILTDLSR"
     gene            1713..2546
                     /locus_tag="VF589_02245"
     CDS             1713..2546
                     /locus_tag="VF589_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8256430.1"
                     /translation="MAIDFTASTVAGTIAGAARAVLLAAEPYDKVRTARAAARAWRAG
                     RLERAFDVAMPDRPARPARPELMPASHMPKRGKGGSERGRIAMLHALAHIEFGAIDLA
                     FDIAGRFGADFPAAFIDDWFGVGADEAMHFVLLDRRLRALGSGYGALPAHDGLWEAAE
                     ATAADPLARLAVVPMVLEARGLDVTPATVARFEAAGDRRSAAVLERIYRDEIRHVRAG
                     TRWFEAACESRKFEPVAEWKRLIDRHFRGVLKPPFNASARDEAGLSRDDSLGVAAQED
                     V"
     gene            2803..>3367
                     /locus_tag="VF589_02250"
     CDS             2803..>3367
                     /locus_tag="VF589_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8256431.1"
                     /translation="MQVSTAAAALLLVLWSVFAAARLMTATPADAAEVARMEAQVEAM
                     QADVQAIKAAADQRFQLTAQEVRKLGLDPKRLQSNNPQYQGTGGPFEAVQPLKADPQF
                     KQLFTSWKKLDQIEQGTIAIPSTQPVRGTSLTSGYGVRSDPFRGRAAMHAGIDLAAPI
                     GTPIYATADAIVGRSQWANGYGNLIELS"
BASE COUNT          508 a         1174 c         1120 g          565 t
ORIGIN      
        1 ccgctcatcc tgaggaggga ctgagccttg gcgaaggccc gtctcgaagg accgctcaga
       61 gcactgtggt ccttcgagac gccgcttcga caagctcagc ggctcctcag gatgagcggt
      121 tctgagccca tcaggatgag cggtttgaat ccatcagatg agcggtttca gcctgtgcgg
      181 ataagcgttt cagcccctca ggctacgccc cccctaccgg ctcaaatccg tcaagatcga
      241 ccgccaatgc gccaaggccg gtgcgatctc cgccactggt atcctctcgt tcaacccgtg
      301 cgcgaagccg tcctcggcgc gcatgaacag gccggagacg ccgtagctcg gcactccggc
      361 ggcgcggaaa tggaggctgt cggtggcgcc cgcggacatg gacggcgtga tctccagctt
      421 cggatagcgc gccttcaccg ctttggtcac cgcctgcatc acgtccggcc gcagcggcga
      481 ggcgtcgctg aacgcgggat cgtcgatcac gctgatcttg accccggggt cggcgaccac
      541 ccgcgccagc tcctgcttca ccgcgtcgat cgaaatgccg gggaagatcc ggcaattgac
      601 gttcgcggtc gcgcgctggg ggagggcgtt caaggcatgc ccggcattgg ccatggtcgc
      661 gacgcaggtg gtgcggatct ggccgacata ttccggccgc gacgagatca aggccgccgc
      721 cgccgcgtcc tcggggttgg cggcatagcg cgccatcgcc gcgccgagct cgccgccgac
      781 ctggcggctc gccgtcttca gcgatgcggt ggtgagcgcg ttggccattg gcgggaaggc
      841 atggtcgcca agccggccga gcgcccgcgc cagccggtag atggcgttgc tgccggtcgg
      901 tgcgctcgaa tggccgccgg ggtcggtgac ttcgagctga taatcggcat aggtcttctc
      961 gcccgcctgg aggccgtaga agatcggcgc gttggcttcg gtgagctggc cgccgccgcc
     1021 gtcgccgttg agcgccagct cggcccccgc gaacttcttc gcgagcgccc gcgtcgtcgc
     1081 catctgggtt tcctcgtcgc ccgacagcgc gaggatcagg gtccgccgcg gcttgtagcc
     1141 cccggccttg aggcgcgcga gcgtcgccac catcatcgac acgtcgaact tgttgtcctc
     1201 cgagccgcgc ccgaaaatat agccattctc ctcgaccgcg gtgaacgggt cgcgctccca
     1261 gtcggcgggc ttggcctcga ccacgtccat atggcccaaa aggacgatcg gcttggcgtc
     1321 gctggtgccg cgataatgag cgaccagggt ggcggtctcg cccatcttcg cgatctcgat
     1381 gtcgctatcg gggaacccgg cctccttcag caccgaggcg agaaaggcgg cataagccgg
     1441 cacctgacct tggccctcga ccgttttgaa ggcgaccgag cgcttcaata tctccttggc
     1501 cttggccgct tcggcggcgg gagcggcggc gggggccgcg gccgccggta aggccgccgc
     1561 cgaaccgagc gccgccgcga tcagccatgc catgccagga cgatttacgc gcattcactt
     1621 gtctcccgaa gatttccggc gcctgtctgc gatggatcgg gcggcgaggc aacagggtgg
     1681 cgccgccgcc gcggctcggg caaggggcgg cgatggcgat cgacttcacg gcctccaccg
     1741 tcgctggcac catcgccggc gcggcgcgcg cggtgctgct cgcggccgag ccttatgaca
     1801 aggtgcgcac cgcccgcgcc gccgcccgcg cgtggcgcgc aggccggctc gagcgggcgt
     1861 tcgacgtcgc catgccggac cggccagcgc gcccggcgcg gcccgagctg atgcccgcct
     1921 cgcacatgcc caagcgcggc aagggcggct cggagcgcgg ccgtatcgcc atgctgcacg
     1981 cgctcgccca tatcgaattc ggcgccatcg acctcgcttt cgacatcgcc ggccggttcg
     2041 gcgctgactt cccggccgcc ttcatcgacg attggttcgg cgtcggcgcg gacgaggcca
     2101 tgcatttcgt tttgctcgac cggcggctga gggcgctcgg ctccggctat ggagcgctgc
     2161 cggcgcatga cgggctgtgg gaagccgccg aggcgaccgc ggctgatccg ctggcgcggc
     2221 tggcggtggt gccgatggtg ctggaagcgc gtgggctcga cgtcactccg gccaccgtcg
     2281 cccggttcga ggcggccggc gaccggcgtt cggcggccgt gctggagcgc atctatcgcg
     2341 acgagatccg gcacgtccgg gcgggcacga gatggttcga agcggcctgc gaatcccgga
     2401 aattcgagcc cgttgcggag tggaaacggt taatagatcg ccacttccgg ggcgtcctga
     2461 agcctccgtt caacgcttca gcgcgcgatg aagccggttt atcccgcgat gactctttag
     2521 gggttgccgc gcaagaggat gtttaacagt ctcagctcac acgtcacggg gtgggcaact
     2581 tcctgacgct tacagccggg caggaatgcc tttgagagac gactcatggt cgccatgtgc
     2641 gcccatgccg accccttcgg gggccgaccg gggatgatta tgacgaatgt ggttaaagcg
     2701 aataatgttc cgggggctct taagttccgt aacgttttca ggccgcgcga ttttttcttt
     2761 catgacggcc gttcgctccg ccgcgtcagc atcggggtcc cggtgcaggt ttccaccgcc
     2821 gccgccgctc tgctcctcgt cctctggtct gtattcgccg ccgcccgcct gatgaccgcc
     2881 acccccgccg acgccgccga agtggcccgc atggaggccc aggtcgaagc gatgcaggcc
     2941 gacgtccagg cgatcaaggc cgccgccgac cagcgtttcc agctcaccgc ccaggaagtc
     3001 cgcaagctcg gcctcgaccc caagcgcctc cagtccaaca acccgcaata tcagggcacc
     3061 ggcggcccgt tcgaagcggt ccagccgctc aaggccgacc cccagttcaa gcagctcttc
     3121 accagctgga agaagctcga ccagatcgag cagggcacga tcgccatccc ctcgacgcag
     3181 ccggtgcgcg gcacctcgct gacctcgggc tacggcgtcc gctccgatcc gttccgcggc
     3241 cgcgctgcga tgcacgccgg catcgatctc gcggcgccga tcggcacgcc gatctacgcg
     3301 accgccgatg ccatcgtcgg ccgctcgcaa tgggccaacg gctacggcaa cctcatcgaa
     3361 ttgagcc
//
LOCUS       DASVRK010000064         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_197226, whole genome shotgun sequence.
ACCESSION   DASVRK010000064 DASVRK010000000
VERSION     DASVRK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197226"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF589_02255"
     CDS             complement(<1..186)
                     /locus_tag="VF589_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256432.1"
                     /translation="MTNKHPAPDEQPVELQDDLDRDPGIGQSKAFFARDGEGGEDLIE
                     GDNTVEGDIENDGGDAGG"
     gene            475..786
                     /locus_tag="VF589_02260"
     CDS             475..786
                     /locus_tag="VF589_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX8256433.1"
                     /translation="MRKFSLAIACAAMAAPVAPVIPTTAAFANHNGYYHGKTWRDSRG
                     RVRCRRANGTVGIIVGGAAGALVGRAVDGGRNRTVGTVVGAAAGALAGREIARSRRRC
                     R"
     gene            994..>1773
                     /locus_tag="VF589_02265"
     CDS             994..>1773
                     /locus_tag="VF589_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX8256434.1"
                     /translation="MEIGILAPGPLNAITDVAGVMVGHRTLVEGDDIRTGVTAILPHG
                     GNLYQDKVPAGFAVANGYGKFMGSTQIEELGEIEAPILLTNTLSVPEAAAAGIEWTLR
                     RPGNAQVRSVNAVVGETNDGHLNYIRARRVTKEDGLAALAAARAGPVAEGSVGAGTGT
                     VAFGWKGGIGTSSRRLPAKLGGWTVGILVQSNYGGVLSVGGVAVGQRLGRYFLKDELS
                     SGSGDGSVIVVIATDAPISDRNLKRLARRAFLGIGRTGSPIT"
BASE COUNT          260 a          631 c          611 g          271 t
ORIGIN      
        1 cccgccggca tcgccgccgt cattctcgat atcgccttcg accgtgttgt cgccctcgat
       61 cagatcttcg ccgccctcgc cgtcgcgggc gaagaaggcc ttggactggc cgatgccggg
      121 atcgcggtcg agatcgtcct ggagctcgac cggctgctcg tcgggggcgg gatgcttgtt
      181 ggtcattcgc tgtctcctct cccgcccggc aacgcggcag gtccgcgcac ggttctgagc
      241 gagcggccag acgagcggaa atggcggtcg tcgacgtacg aacccggacc gggcgaatgc
      301 gtgccttggg tcggagcctt ctccgccaac gcggcggagc gccgaacggt tctgcgggca
      361 cggtcagccg agcggaaacg gcggttatcg gggatcgttg tctcaggtta atgaaactgg
      421 cggcaacctc gcccgttctt aggccgagtt ttcacgggga aaggaaccca agtcatgcgt
      481 aaattcagcc tcgcaatcgc ctgtgccgcc atggcggccc cggtcgcccc ggtcattccg
      541 accaccgcgg ccttcgccaa tcacaacggc tattatcacg gcaagacctg gcgcgattcg
      601 cgtggccgcg ttcgctgccg ccgggccaac ggcacggtcg ggatcatcgt cggcggcgcc
      661 gccggcgccc tcgtcggccg ggcggtcgac ggcggccgca accgcaccgt cggtaccgtc
      721 gtcggcgccg ccgcgggcgc tttggcaggg cgcgagatcg cccgcagccg ccgccgctgc
      781 cgctaagcca tcggccggtg aaacctggaa gcggggcgcc tcccatcgga ggcgccccgt
      841 tttcatgtct ggcgaagagc gcgggaatgc ggcagcctgc cggcgatgga gaaaagctat
      901 cgcgcctcta gctgctttcg cgccgccgcc gcggccctgc tcctggtgac cgcggccgcc
      961 gaagccgggc gcccgcgtgc tcgggaggcg ggcgtggaga tcggaatcct cgcgcccggc
     1021 ccgctcaacg ccatcaccga tgtcgccgga gtgatggtgg gccatcgcac cctggtcgag
     1081 ggggacgata tccgcaccgg ggtgaccgcg atcctcccgc acggcggcaa tctctaccag
     1141 gacaaggtac ccgccggctt cgccgtcgcc aacggctatg gcaagttcat gggctcgacc
     1201 cagatcgagg agctcggcga gatcgaggcg ccgatcctcc tcaccaacac cctctccgtc
     1261 cccgaagccg cagccgccgg gatcgaatgg acccttcgcc ggcccggcaa cgcgcaggtg
     1321 cgctccgtca acgccgtggt gggcgagacc aacgacggcc atctcaatta catccgcgcc
     1381 cggcgggtga cgaaggagga cggcctcgcc gccctcgccg ccgcccgcgc cgggccggtg
     1441 gcggaaggct cggtcggggc tgggaccggc accgtcgcct tcggctggaa gggcgggatc
     1501 ggcaccagct cgaggcggct accggcgaag ctcggcggct ggaccgtggg aatcctcgtc
     1561 cagagcaatt acggcggcgt gctgagcgtc ggcggagtcg ccgtcgggca gaggctggga
     1621 cgctatttcc ttaaggacga gctgtcctcg ggctccggcg acggctccgt catcgtggtg
     1681 atcgccaccg acgcgcccat ctccgaccgc aatctcaagc ggctagcccg ccgggccttc
     1741 ctcgggatcg gccggaccgg ctcgccgatc acc
//
LOCUS       DASVRK010000065         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_260326, whole genome shotgun sequence.
ACCESSION   DASVRK010000065 DASVRK010000000
VERSION     DASVRK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260326"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1730
                     /locus_tag="VF589_02270"
     CDS             <1..>1730
                     /locus_tag="VF589_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8256435.1"
                     /translation="PPLPLHAQEAPPLPLNAQQAAPLPLHAQEAPPLPLHAQEAPPLP
                     LHAREAPPLPLHAQEAPPPADEAAAPSASDEDLLDEEEAGEEIVVTGQRLRGAVVGDI
                     PPEVQLDARDIRAYGAGSLAELMTALEPQTRSGAGRGGGRPVILLNGRRISGFSEIRD
                     LPPEAVERVDILPEEVALKYGYRADQRVVNFVLRPRFRAVTAEAAAGMATEGGRGSYE
                     ADLNVLRIMNNGRWNLDAEVRRDSALFESERDLIQAQATAGGGADGTDLGRFRTLLPQ
                     TDRLSLAGTVNRTILNDVSATLNARFDQTSSESRFGLPAAAFALPTSAGDGIRPLTRE
                     SDSRATHLGLSMNGDISPWRWSLTANYDRSNNRTLSDTNADPAEAQAGMLYGARERSS
                     AISQSADAELVAHGSLITLPAGDISTSVKVGLDTRSLHSESNRGGTDQVRDLSRNRAN
                     GQVNIDVPLASRRNEVLSAIGNLSLNANAAVEHLSDFGTLRVLGGGINWSPIEGVRVL
                     ASLSDEDGAPTIQQLGDPVVLVPNVRVFDFTRGQTVDISRIEGGNPDLSADNRRVFKI
                     SANVKPWEER"
BASE COUNT          316 a          621 c          552 g          241 t
ORIGIN      
        1 ccgccgctgc cgctccacgc ccaggaagct ccaccgctgc cgctcaacgc ccagcaagca
       61 gcgccgctgc cgctccacgc ccaggaagct ccgccgctgc cgctccacgc ccaggaagct
      121 ccgccgctgc cgctccacgc ccgggaagct ccgccgctgc cgctccacgc ccaggaagcc
      181 ccgccgcccg cggatgaagc ggccgccccg tcggcaagcg acgaagatct cctcgacgag
      241 gaggaagccg gcgaggagat cgtcgtcacc ggccagcgcc tgcgcggcgc ggtcgtcggc
      301 gacatccctc ccgaggtcca actcgacgcg cgagatatcc gcgcctacgg cgccggcagc
      361 ctcgcggaac tgatgaccgc gctggagccg cagacccgca gcggcgccgg ccggggagga
      421 ggccgtccgg tcatactcct gaacggccgt cggatctcag gcttctccga aatacgcgat
      481 ttgccgcccg aagccgtcga gcgggtcgac atccttccag aggaagtggc gctcaaatac
      541 ggctacaggg ccgatcagag ggtcgtgaac ttcgtgcttc ggccgcgctt ccgggcagtc
      601 actgccgaag cggcggccgg catggccacc gaaggcgggc gcggaagcta cgaagccgat
      661 ctgaacgtgc ttcggatcat gaacaacggc cgctggaacc tggatgcgga ggtccgccgc
      721 gactccgcct tgttcgaaag cgagcgagat cttattcagg cacaagccac ggccggcggc
      781 ggagccgacg gcaccgacct cggccggttc cgcaccctcc tgccgcagac ggaccggctg
      841 tcgctggcgg ggaccgtcaa ccgcacgatc ctgaacgacg tttcggcgac gctcaacgcg
      901 cgcttcgacc agacctcgag cgaaagccgc ttcggcctgc ccgccgcggc cttcgcgctt
      961 cccacttcgg ccggtgacgg cattcggccg ctgacgcgcg aatccgacag ccgcgctacc
     1021 catctgggct tgtcgatgaa tggcgacata tcgccatggc gatggtcgct caccgccaat
     1081 tacgacaggt ccaacaaccg taccctcagc gacaccaatg ccgatccggc ggaagcccag
     1141 gcaggaatgc tctacggtgc acgggagaga tcgagcgcga tcagccagtc cgcggacgcc
     1201 gaactcgtcg cccacggctc gctcatcacc cttccggccg gggacatttc cacgagcgtc
     1261 aaggtcggcc tggacacgcg cagcctgcac agcgagagca atcgcggcgg cacggaccag
     1321 gtgcgcgacc tttcgcgcaa ccgagccaac ggccaggtca atatcgacgt cccgctcgca
     1381 agccggcgca acgaggtgct gagcgcgatc ggtaatctgt cgctcaacgc caatgccgcg
     1441 gtcgagcatc tctcggattt cgggactctg cgagtgctcg gcgggggaat caactggtcg
     1501 ccgatcgagg gagttcgagt gctcgcgtca ttgtccgatg aagacggagc gccgaccatc
     1561 cagcagctcg gcgacccggt ggtcctggtg cccaatgtgc gggttttcga cttcacccgc
     1621 gggcaaaccg tcgacatcag ccggatcgag ggcggcaacc ccgacctttc cgcggacaac
     1681 cggcgggtct tcaaaatcag cgcgaacgtg aagccttggg aggaaaggga
//
LOCUS       DASVRK010000066         6762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_446589, whole genome shotgun sequence.
ACCESSION   DASVRK010000066 DASVRK010000000
VERSION     DASVRK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446589"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1929)
                     /locus_tag="VF589_02275"
     CDS             complement(<1..1929)
                     /locus_tag="VF589_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256436.1"
                     /translation="MTHAIARTAARTIAAALLATTALASPAFGQAAGPLERQAPDENG
                     VDVVTGKMNMAPVTLSAGPPGEGLTFVSGFAGGRRYDSFNVELEADPDKVCVVIGGHR
                     RCFTRTTPQGLEIFTSREGDGATLTHQFAGTATERYTYTDRNGTVFLFLNEYGDAVGT
                     ITARLRTITAPSGELVTYTYRKEGTTARAQSVVNNRGYQLKPTYASDVWETTGTNAAF
                     KRLTKVTAINNAVEYCAPGANSCTLTQDWPSLDVASTSKTLNFTDKTGKNHFYMLAGG
                     ELVSATKPGDIVAFAVEYDEANRVKLLSRGSGNWTYSYVDHEVQGKRTTRVTPPLLGS
                     AAPRTYVSDLSSRRVESVTDELGRKASFGYDGSGRMTSMTLPEGNGIELTYDARGNVE
                     EQRVKSKPDEAGARLPDIITSADYPDSCTNPNTCNKPISTTDARGFTTNYSYSSVHDG
                     LLSITSPAPSTGAARPQVRYSYSLLRARYKDNSLALVDGPQIYRLTGVSTCHTGAVTD
                     TETTCIGKASETRTTIGYGDPDPLQGHNLLATAVTQAAGDGTLTATTRYGYDHAGRLT
                     ALDGPLFGAADMIRYRYDAAGRRVGTIYPDPDGAGPMKRRAERIVHDFDSLSFTTQSG
                     TVEGPTVTDWNAFTPLEQV"
     gene            complement(2009..2857)
                     /locus_tag="VF589_02280"
     CDS             complement(2009..2857)
                     /locus_tag="VF589_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX8256437.1"
                     /translation="MNRWTILAAAAALPAAAVPAVTLASETVTFGYDVRGRLITVTRA
                     GGTNDGIVSGYSHDQADNRELLAVTDATAPTFSVREVYAVEGDDIVFTISKSGTTSGT
                     VGVSFQTGDKTAIAGSDYTARPLTALSFAPAETSKTVRVATSADDQPDDDERMYLELS
                     APTGGAVIAVARAIGTIWEKAPNPGPTTAPDTGSLSRCSAAGTFNVTANDQDPQGPVT
                     LKAVSRGALGGASVASTTSVGYLPGNVAGKERLTYTVEDALGARSQGTLTLTLGPGSG
                     CPADNE"
     gene            2996..4387
                     /locus_tag="VF589_02285"
     CDS             2996..4387
                     /locus_tag="VF589_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX8256438.1"
                     /translation="MTDLSALLRPDQGQPANALILLDRKGLEPWLKAQPERVRRAAET
                     AGFEGKADQFALVPGDKAADWAALLGAADAADPGVWGLAKAADLLPAGSYRLAGRAPG
                     PAALGWLLAQYRFDRYKAEPAAKGPRILLSEEPARIAEALLMAEATNLVRDLVNIPAQ
                     DMGPDALESAVSELAKSCSAALSPTSGAELEREYPLIHAVGRAAAPGRGPRLLELEWG
                     DPRHPRLALIGKGVAFDTGGLDIKNSAGMRLMKKDMGGAAHAIALALLVMKSRLPVRL
                     HLLVPAVENAIAGNAFRPGDILKSRKGVTVEVHNTDAEGRLILADALTRAAESRPDLI
                     LDFATLTGAARVALGPDLPAMFANRDDLAAALLAAGEAEADPVWRMPLWKPYREMLAS
                     DVADLANASDAPMAGAVTAALFLQKFVPDDLAWAHFDTFAWRAAAKPGRPKGGDALGL
                     RAAWAMLRSRYRA"
     gene            4498..4866
                     /locus_tag="VF589_02290"
     CDS             4498..4866
                     /locus_tag="VF589_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8256439.1"
                     /translation="MSERSLKSWMQTLISYVRSGQPDLTNRQMALMLLVYLTPGPHTV
                     RGLANILGVSKPVITRALNTLGSLGYLRRVRDEADRRNVFVARTNTGQEFLEGFERNI
                     EQDGAPRRTAKERGLILQHG"
     gene            4781..5701
                     /locus_tag="VF589_02295"
     CDS             4781..5701
                     /locus_tag="VF589_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX8256440.1"
                     /translation="MKASNGISSRTAPHAGPPRNGASSSSTAERFTLAGPSLPLDERV
                     HAYRRDLADIALAGQLFAPHYARPLTKACGTRPAFVYPEGSDGAKASSELLPGEEFAL
                     LEVSGHWGWGYCRHDHYVGYVEAIALVEPLPPTHIVAAAAAAILAEPDVHAATLASLP
                     MGARVTGHESRGYLATDLGFIAFTQVRAVDAFEHDPVAVAERLLGAPYLLGGRTSTGI
                     DCSGLIQLSLGLCGIPAPRDSDQQRVLGTALAEGAPLERGDLLFFEGHVGLMVDGESL
                     IHATGHVGSVVVESLAHVAARTPVLDRRRL"
     gene            complement(5713..6642)
                     /gene="mltG"
                     /locus_tag="VF589_02300"
     CDS             complement(5713..6642)
                     /gene="mltG"
                     /locus_tag="VF589_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX8256441.1"
                     /translation="MAGAGAAYRAFWAGPGPAAQPKTIIVEQGSSLGKVAEQLEKQGV
                     IRGGARSFRTLARLFGSKDPIQAGEFEIPAGLSAGSVLDHLQHGRPVQRLVTIPEGMP
                     SVLVFERLMRVPYLTGNVAVPEEGTVLPNSYSYQRGESRAAVLKRMQAAMTKELDALW
                     GKRQPHSAVTSKRDAITLASVVEKETGKPSERRMVAGVYSNRLKQGMKLDADPTVIYP
                     ITKGKPLGRRILRSELNADNGYNTYARAGLPAGPIANPGRESIAAVLNPAPTQALFFV
                     ADGTGGHVFANTLAEHNANVRKWYALRRQRGEM"
BASE COUNT          948 a         2388 c         2267 g         1159 t
ORIGIN      
        1 gacctgctcg agcggcgtga aggcgttcca atcggtgacc gtggggccct cgaccgtgcc
       61 gctctgggtg gtgaaggaca ggctgtcgaa atcgtggacg atccgctccg cccgccgctt
      121 catcgggccc gcgccgtccg ggtccggata gatggtgccc acccgccgcc cggccgcgtc
      181 gtagcggtag cggatcatgt ccgcggcccc gaacaacggc ccgtccagcg ccgtcagccg
      241 gccggcatgg tcatagccgt agcgggtggt ggcggtgagg gtgccgtcgc cggccgcttg
      301 ggtgaccgcg gtggccagca gattgtggcc ttggagcggg tccgggtcgc catagccgat
      361 cgtcgtcctg gtctcgctcg ccttgccgat gcaggtcgtc tccgtatcgg tcaccgctcc
      421 ggtgtggcag gtcgagacac cggtcagccg atagatttgc ggcccgtcga cgagggccag
      481 cgaattgtct ttgtagcgcg cccgcagcag cgaatagctg tagcgaacct gcggccgcgc
      541 cgcccccgtc gacggcgccg gcgaagtgat gctgagcaac ccgtcatgga cgctgctata
      601 gctataattg gtcgtgaagc cgcgcgcgtc ggtggtcgag atcggcttgt tgcaggtatt
      661 ggggttggtg cagctgtccg gatagtcggc cgaggtgatg atgtcgggca ggcgcgcgcc
      721 ggcctcgtcc ggcttcgatt tcacccgctg ctcctcgacg ttgccgcggg cgtcataggt
      781 gagctcgatc ccgttcccct cgggcagcgt catgctcgtc atccggcccg atccgtcata
      841 gccgaagctt gccttgcggc cgagctcgtc ggtgaccgat tccacccggc gcgacgacag
      901 gtcggagacg taggtcctgg gggcggcgct cccgagcagc ggcggcgtca cgcgggtcgt
      961 gcgcttgccc tgcacctcgt ggtcgacgta ggaataggtc cagttgccac tgccgcggct
     1021 gagcagcttc acccggttgg cctcgtcata ttccaccgcg aaggcgacaa tgtcgcccgg
     1081 cttggttgcc gacaccagtt cgccgcccgc cagcatgtag aaatggttct tgccggtctt
     1141 gtcggtgaag ttcagggttt tgcttgtgga ggccacgtcg agcgacggcc aatcctgcgt
     1201 cagcgtgcag ctattggcgc cgggcgcgca atattcgacc gcattgttga tcgcggtgac
     1261 cttggtcagc cgcttgaacg cggcattggt gccggtcgtc tcccagacgt ccgacgcata
     1321 ggtcggcttc agctggtagc cgcgattgtt gaccaccgac tgcgcccgcg cggtagtgcc
     1381 ctccttgcga taggtgtagg tgacgagctc gccgctcggc gcggtgatgg tccggagccg
     1441 cgccgtgatc gtgccgaccg catcgccata ttcgttgagg aacaggaaga cggtgccgtt
     1501 gcggtcggta taggtatagc gctcggtcgc ggtgccggcg aactggtggg tcagcgtggc
     1561 gccgtcgccc tcgcgcgagg tgaatatttc gaggccctgc ggggtcgttc gggtgaagca
     1621 gcgccggtgc ccgccgatca cgacgcagac cttgtcgggg tccgcttcca gctcgacgtt
     1681 gaagctgtcg tagcggcggc cgccggcgaa gccgctgacg aaggtcagcc cctcgcccgg
     1741 cgggccggcg ctgagcgtca ccggcgccat gttcatcttg ccggtgacga cgtcgacgcc
     1801 attctcgtcg ggcgcctggc gctcgagcgg gcccgccgcc tgcccgaagg cggggctggc
     1861 gagagcggtg gtggcgagca gggccgcggc gatcgttcgg gcggcggtgc gggcgatggc
     1921 atgggtcatg ataggtctcc gggaaagcag gatggggatg gcggccgagg ctgggcttcg
     1981 gcgcatggct ggatcgggct ggggcgcttc attcattgtc tgccgggcag cccgaaccgg
     2041 ggccgagcgt cagcgtgagc gtgccctgcg atcgcgcgcc gagggcgtcc tccaccgtgt
     2101 atgtgaggcg ctctttgccg gcgacgttgc cgggcaagta gccgaccgac gtcgtgctcg
     2161 cgacgctggc gcccccaagc gctcctctgc tgacggcttt cagcgtgacc ggcccctggg
     2221 ggtcctggtc gttggcggtg acgttgaagg tgccggcggc ggaacagcgc gagagactgc
     2281 ccgtgtccgg ggcggtcgtc ggccccgggt tcggcgcctt ctcccagatc gtgccgatcg
     2341 cccgcgccac cgcgatcacg gcgccgccgg tcggagcgga aagctccaag tacatgcgtt
     2401 cgtcgtcgtc cggctggtcg tcggcgctcg ttgccacccg taccgtcttg ctggtctcgg
     2461 ccggcgcgaa gctcagcgcg gtcagcgggc gggccgtgta atccgatccg gcgatcgcgg
     2521 tcttgtcgcc ggtctggaag ctcaccccga ccgtgccgga ggtggtgccg ctcttgctga
     2581 tcgtgaagac gatgtcgtcc ccctcgaccg catagacttc cctgacgctg aaggttggcg
     2641 cggtcgcgtc ggtgacggcc agcagctcgc gattgtcggc ctggtcatgg ctgtagccgg
     2701 agacgatccc gtcattggtg ccgccggccc gcgtcaccgt gatgagccgc ccgcgcacgt
     2761 cgtagccgaa ggtcaccgtc tccgacgcga gcgtgaccgc cgggaccgcg gccgccggga
     2821 gcgccgctgc cgcggccagg atcgtccatc tgttcatcgt tccgcctttt cgtcatcgcc
     2881 cgattcgcgg gccagaacga aagaagaaca tttgaaatcc tacattacaa gcgcggcgcc
     2941 tcgccgccgt ccgcggcggg tttcgcggcg ccggcaaatt gcctaaggcg ccgccatgac
     3001 cgacctttcc gcgctcctcc ggcccgacca gggccagccc gccaatgcac tcatcctgct
     3061 cgacaggaag gggctggagc cgtggctgaa ggcgcagccc gagcgggtgc ggcgcgccgc
     3121 cgagacggcc gggttcgagg gcaaggccga tcagttcgca ttggtgccgg gcgacaaggc
     3181 cgccgactgg gcggcgctgc tcggcgccgc cgacgccgcc gatccgggcg tgtggggtct
     3241 cgccaaagcg gccgatttgc tgcccgcggg cagctaccgg ctcgccggcc gcgcaccggg
     3301 gccggccgcg ctcggctggc tgctcgccca atatcgcttc gaccgctaca aggccgagcc
     3361 cgccgcgaag gggccgcgga tcctgctcag cgaggagcct gcgcgcatcg ccgaagcctt
     3421 gcttatggcc gaagccacca acctggtgcg cgatctcgtc aacataccgg cacaagatat
     3481 gggccccgac gcgctggaat ccgccgtttc cgagcttgca aagtcatgct ccgcggcact
     3541 ttcgccgaca tccggcgccg aactcgagcg cgaatatccg ctcatccacg ccgtcggccg
     3601 cgccgccgcc ccgggccgcg gcccccgcct cctcgagctc gaatggggcg acccccgcca
     3661 cccccgcctc gccctcattg gcaagggcgt cgccttcgac acgggcggcc tcgacatcaa
     3721 gaatagcgcc ggaatgcgcc tcatgaagaa ggatatgggc ggcgccgccc atgccatcgc
     3781 gctggccctt ctggtcatga aaagccgcct ccccgtccgc ctccatttgc tcgtccccgc
     3841 ggtcgagaac gcgatcgccg gcaacgcctt ccgccccggc gatatcttga agagccgcaa
     3901 gggtgtgacc gtcgaggttc acaatacgga tgcggaaggc cggctgatcc tcgccgacgc
     3961 cctcacccgc gccgccgaat cccgtcccga cctcatcctc gatttcgcga ccctgaccgg
     4021 cgccgcccgc gtcgcgctcg gccccgacct cccggccatg ttcgccaatc gcgacgatct
     4081 cgccgccgcc ctcctcgccg ccggcgaggc ggaggcggac ccggtctggc ggatgccgct
     4141 ctggaaaccg tatcgcgaaa tgctcgcctc cgacgtcgcc gacctcgcca atgccagcga
     4201 cgcgccgatg gccggcgccg tgacggccgc ccttttcctc cagaaattcg tgcccgacga
     4261 cctcgcctgg gcccatttcg acaccttcgc ctggcgcgcc gccgccaagc ccggccggcc
     4321 taaggggggc gacgcgctcg gccttcgcgc cgcctgggcg atgctccgct cgcgctaccg
     4381 cgcctgagac cgacgcccct gcttttgcgt tgaaaatgcc ggtggttaac gaaacgaaac
     4441 ctgttctgct tggttgtagt gccgaaacgt acctcgcgcg gaaatcggag aaggatcgtg
     4501 tcggaacgct cgctgaagag ctggatgcag acgctaatca gctatgtgcg gtcgggccag
     4561 ccggacctta ccaaccggca gatggccttg atgctgctgg tctatttgac gcccgggccg
     4621 catacggtcc ggggcctcgc caacatcctc ggcgtgtcca agccggtgat cacgcgcgcc
     4681 ttgaacacgc tcggctcgct cggctatctg cggcgggtcc gcgacgaggc ggatcggcgg
     4741 aacgtcttcg tcgccaggac caatacgggg caggaattcc ttgaaggctt cgaacggaat
     4801 atcgagcagg acggcgcccc acgccggacc gccaaggaac ggggcctcat cctccagcac
     4861 ggctgaacgc ttcaccctcg ccggcccttc gctgccgctc gacgagcgcg tccacgccta
     4921 tcgccgcgac ctcgccgaca tcgccctggc ggggcaattg ttcgcccccc attatgcgcg
     4981 cccgctcacc aaggcatgcg gcacccgccc ggccttcgtc tatccggaag gctctgacgg
     5041 cgcgaaggcg agttcggagc tgctcccggg cgaggagttc gcgctcctcg aagtgtccgg
     5101 ccactggggc tggggctatt gccgccatga ccattatgtc ggctatgtcg aggcgatcgc
     5161 cctcgtcgaa ccgctgccgc cgacccatat cgtcgccgcc gccgccgccg ctatcctggc
     5221 cgagccggac gtccacgccg ccaccctcgc cagcctgccg atgggcgcgc gcgtcaccgg
     5281 ccacgaaagc cgcggctatc tcgccaccga tctcggcttc atcgccttca cccaggttcg
     5341 cgccgtcgac gccttcgagc atgatccggt cgcggtcgcc gagcggctcc tcggagcgcc
     5401 ctatctgctc ggcgggcgca ccagcaccgg catcgactgc tcgggcctca ttcagctctc
     5461 gctcggcctg tgcggcatcc ccgccccccg cgacagcgat cagcagcggg tgctcggcac
     5521 ggcgctggcc gaaggtgccc cgctggagcg cggcgacctc ctcttcttcg aaggccatgt
     5581 cggcctgatg gtcgatggcg agtcgctgat ccacgcgacc ggccatgtcg gcagcgtggt
     5641 cgtggagtcg ctcgcccatg tcgcagcccg cacgcctgta ctcgatcgtc gtcggctcta
     5701 gccctcctgg ccctacatct ctccgcgctg gcggcggagg gcataccatt tgcggacatt
     5761 ggcattatgc tcggccagcg tgttggcaaa gacgtggcct ccggtcccgt cggcgacgaa
     5821 gaagagcgcc tgggtcggcg ctgggttgag caccgccgcg atcgactccc gccccggatt
     5881 ggcgatcggc cccgccggaa ggccggcccg ggcataggtg ttgtagccgt tgtcggcatt
     5941 gagctcggac cgcaggatcc ggcgcccgag cggcttgccc ttggtgatcg ggtagatgac
     6001 ggtggggtcc gcatccagct tcatgccctg cttcagccgg ttcgaataga cgcccgccac
     6061 catccgccgt tcggacggct tgccggtttc cttctcgacc accgaggcga gggtgatggc
     6121 gtcccgcttc gaggtgacgg cgctatgcgg ctgccgcttc ccccagagcg cgtcgagctc
     6181 cttggtcatc gccgcctgca ttcgcttcag caccgccgcc cggctctcgc cgcgctgata
     6241 gctgtagctg ttgggaagca ccgtcccttc ctcgggaacg gcgacgttgc cggtgaggta
     6301 gggcacccgc atcagccgct cgaacaccag gaccgacggc atcccctcgg ggatggtgac
     6361 cagccgctgc accggccgcc catgctggag atggtcgagc acgctcccgg cgctgagccc
     6421 ggccgggatc tcgaactcgc cggcctggat cggatccttc gacccgaaca gccgcgccag
     6481 cgtgcggaag cttcgcgcgc ccccgcggat gacgccctgt ttctcgagct gctccgccac
     6541 cttgccgagg ctcgatccct gctcgacaat gatcgtcttg ggctgcgcgg ccggacccgg
     6601 cccggcccag aacgccctat aagccgcgcc cgcgccggcc accaccgcga tcagcagcgc
     6661 ggccagcagc cacttgaccg ggaagcttct agacagcgcg catcaccagg ctggcgttgg
     6721 tgccgccgaa gccgaagctg ttgttgagca ccgccttgac ct
//
LOCUS       DASVRK010000067         9354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_446626, whole genome shotgun sequence.
ACCESSION   DASVRK010000067 DASVRK010000000
VERSION     DASVRK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446626"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VF589_02305"
     CDS             complement(<1..618)
                     /locus_tag="VF589_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256442.1"
                     /translation="MPIDAYQPTDVDDIPPWPRPWPWPPRPPHQPRPPVPPIPPIGAL
                     DARDAAETVPLNDANFAVVMADAAKFASLNDENNLTGHKSWHGLGWAILNYILSNFDV
                     SENQQKSLEATDGALIEIVESELAQVALLHSAGRDVTFTALGLNPDNCPGLPLETVMV
                     RSDVSSAGGKVKENRKIVFLRGHLPIKPDKPDKPPKEPRPSPTPGP"
     gene            1134..1520
                     /locus_tag="VF589_02310"
     CDS             1134..1520
                     /locus_tag="VF589_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256443.1"
                     /translation="MYQTGGTAKEIATRAGTCISQHLAPGTVNAQLIISSDLDGGVIV
                     ARSALEYGGLVQWKIRSNFTFEAREGRFRIVQTNLERFNDQFDVGWKPIGAWTSSQWK
                     GAEKAFSTSATVVAQCVINGLKRETW"
     gene            complement(1635..1711)
                     /locus_tag="VF589_02315"
     tRNA            complement(1635..1711)
                     /locus_tag="VF589_02315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1675..1677),aa:Arg,seq:acg)
     gene            1810..2322
                     /locus_tag="VF589_02320"
     CDS             1810..2322
                     /locus_tag="VF589_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256444.1"
                     /translation="MDARRPFPPLAAAAALLAGCAAEGPFPSLAPRAVERQLANEQPP
                     APPSIIPSDPATAGRIRDMVAAAREGDGAFRSALAAARPAVAGAGAAGSESWVAAQQA
                     VSRAQAARAPTVNALADLDSLAVRLAAARPAASQADIAAAVAAVETVQAIADAQEDAL
                     AGLRARLSGR"
     gene            complement(2309..2860)
                     /locus_tag="VF589_02325"
     CDS             complement(2309..2860)
                     /locus_tag="VF589_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX8256445.1"
                     /translation="MGGPHLLSFGGRAPRVDPAAFVAPGAQLIGDVEIGPEASIWYNC
                     VLRGDVNRIRVGARTNIQDGSVIHVDSPKAGDAAGHPTLIGEDVLIGHLAMVHGCILH
                     DRAFVGLGAIVMDGCVVESGAMLAAGAMLTPGKRIPAGQLWAGRPAKYVRDLTPEELA
                     GQQAGVAHYVELAKAHKAALSGR"
     gene            complement(2839..3387)
                     /locus_tag="VF589_02330"
     CDS             complement(2839..3387)
                     /locus_tag="VF589_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8256446.1"
                     /translation="MAMIETERLTLRSWRDGDATSFERHTNTPAVMRWLGGAKTPDFI
                     AGVVARLAAYEQERGFTFWAVERKADGELLGFCGLKIANDAGSPVEGEYEAGWRFRED
                     VWGQGYAKEAAIASLEAAFGTFGAERVVSLTVDGNAPSWGLMERLGMTRRPELDYEGP
                     DWAESTVIVYAIGREEWAARTS"
     gene            complement(3406..5640)
                     /locus_tag="VF589_02335"
     CDS             complement(3406..5640)
                     /locus_tag="VF589_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX8256447.1"
                     /translation="MTAEAGAAPSAREAYARPLFFATIVTGSFLLFLTQPMIARMALP
                     RLGGAPAVWNSAMMVYQALLLGGYAYAHQLARLRPRTQAGLHLGLFAIAALWLPIGLG
                     AALPPGGVSPALWTPWLLLSSIGPLFFIVSSQAPLMQRWYALESARGEPYALYAASNL
                     GSFAGLISYPLLVEPLLTLDQQSALWTGGYALLVLLVVGCALTIPGQAVEAVPEPASP
                     APSRRRVGLWIALAAVPSGLMLSTTTHLTTDIVAMPMLWALPLGLYLLSFVIAFARRR
                     GAAEFIGQVAPLVLLIAGGLAFADGSRNPLAAAALGLSLLFFVAVALHAEMYRLRPEP
                     DRLTGFYLAMSVGGVLGGAFCALAAPLLFDWSYEHPLLILAAALLLPQRNYLKRIERI
                     WSEPKRGFVLAMAMPVAVFLLSLVGDRRFLFEAPVWVAVAASGAIGAAALFSLGRPVV
                     FAACLAALMMSYGGWSNVHRSYAGVRTRSYFGIYSVGTDADRNALSLTHGTTLHGLQN
                     ITRGRELEPTTYYARKSGVGLALGAAERLFGQRARIGVVGLGTGTLACYARPGQRWRF
                     FEIDPAMVRLARESGYFTFLNSCAPQAQMVIGDARLTLARQPAAGLDMLAIDAFSSDS
                     VPMHLLTREAFQVYARALQPRGILLVHISNRYLDLEPVVAAAAAQGGWAASKLHYFPD
                     EDEGNATASVWIAMARSPEALDDMFLGIGVFAPWEELEPRPGFEGWSDDFASILPLIE
                     GWTD"
     gene            complement(5630..6628)
                     /gene="hemB"
                     /locus_tag="VF589_02340"
     CDS             complement(5630..6628)
                     /gene="hemB"
                     /locus_tag="VF589_02340"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX8256448.1"
                     /translation="MAAASFPAVRLRRTRSAAWSRAMVAETVLTPSDLIWPLFVTGGH
                     AVEEPIATLPGVSRWSIDFIPERAREAQRLGIPCIALFPNTPAELRTEGGQEALNPDN
                     LMCRAIKAAKDAAPDVGILTDVALDPYTADGHDGIADEAGYVLNDETSKMLVEQALVQ
                     AAAGADIVAPSDMMDGRVGAIRQALEREGHVNVQIMAYAAKYASAFYGPFRDAVGSAG
                     RLKGDKKSYQMDPANAIEALREVALDIAEGADHVMVKPGLAYLDIVRRVKERFEVPVF
                     AYQVSGEYAMIEAAAAAGAGDRDALLMETLTAFKRAGCAGVLSYHAPVAARLLRDR"
     gene            6830..7297
                     /locus_tag="VF589_02345"
     CDS             6830..7297
                     /locus_tag="VF589_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256449.1"
                     /translation="MKLLKPLATLSAGAMLAAIAASAAAQPVAPPPAAPPPLPQLYLL
                     GQEPYSVSGRNFVRYRYDVHNKDKYPAELFAAAPNLPPCGLNANSSRTWVDIVEQGGR
                     RIYGFCALGDPQYLGQIWFAMEEGTIPPSWIYIEMTDRQTNTKYRSNLADTTL"
     gene            7393..8286
                     /locus_tag="VF589_02350"
     CDS             7393..8286
                     /locus_tag="VF589_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8256450.1"
                     /translation="MSLIKPVPKEAVLVVNAHSRKGEELFREATAKLEMSGVRLIESY
                     PVRDPDELIPTVRKAVAAGAPMVIVGGGDGSLSSTVDDVVGRDCVFALLPLGTANSFA
                     RTLGIPLDLDGAIATIATGKRRRIDLGVINGDYFANAAAIGLSPMIGDTVPHELKKYL
                     GRFGYLLWAVWCFARFGPFRLFVDDGTKVHRLWTTEVRIFNGRFHGGVELTETTDVDS
                     GDIVIQAVTGRGLARLVWDWYAKFFKLSSRDANTVEFRGKSLHVDTRPHHRISIDGEI
                     VARTPATVAIASRAIEVVVPA"
     gene            complement(8283..>9354)
                     /locus_tag="VF589_02355"
     CDS             complement(8283..>9354)
                     /locus_tag="VF589_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8256451.1"
                     /translation="QGAKMSKSKGNTVDPLGLVDRYGADALRFTLAAMESQGRDIKLD
                     EKRVEGYRNFATKLWNAARFCQANGIGASAGLEPPAAELPVNKWIVGETVKTVQALDL
                     AMAELRFDESANLIYQFTWATFCDWYLELIKPVSSGDERGRVDEETRAVAGWVLDQIL
                     VMLHPFMPFITEELWNAMGERPYDLIVAKWPMPDARRLDPEAGPEVDWLIRLIGGIRA
                     ARTELGVPPGAKLALHVKDAGVQTLERLRRNDGAIRRLARIEAVAEGEAPAGGAAQLV
                     VDEATFVLPLEGVIDIAAERARLAKALQAAEKERDALAARLGNPSFVERAKPEAVEKA
                     RSDHAEKASDAEKYRAALARLG"
BASE COUNT         1573 a         3323 c         3025 g         1433 t
ORIGIN      
        1 aggtccgggg gtgggggagg gccgaggttc tttcggtggt ttgtctggct tgtcgggctt
       61 gattggcagg tgccctctca ggaatacaat tttccgattc tctttcactt ttccgcctgc
      121 cgacgagaca tcgctccgca ccatcaccgt ttcgagcggc agccctgggc aattatccgg
      181 attaagacct aatgctgtga aagtgacgtc acggccggcg gaatgcagca gagccacttg
      241 agctagctcg ctctcgacaa tttctatgag cgcaccgtct gtggcttcga gtgacttctg
      301 ctgattctca ctaacatcga agttactgag gatgtaattc aagatagccc accccaagcc
      361 atgccaggat ttatgaccag tcagattgtt ctcatcattc agtgaagcga acttagcagc
      421 gtccgccatg acaactgcaa agttagcatc gtttagggga acggtctctg cagcgtcacg
      481 cgcatccagt gcgccgatcg gaggaatggg agggaccggc gggcgcggct gatggggagg
      541 gcgtggtggc cacggccaag gccttggcca aggcgggatg tcatcgacgt cagtcggttg
      601 atacgcatca atcggcatgt aaaccctcca tctgtctgtg gttgtcgagt ttctccttcg
      661 cccgcttacc aggcctctcc gttgtgcagc agatgaccgg tggcttgcat aatgggactc
      721 atagacttgt ggaagtaggc gctcgcttgc gaagtcctca agaccaagca cactacacct
      781 tcggtattcg atcgggtgca gcagcggggg ccctaactcc ttcgcccctg acgctgccag
      841 cgcatgcatc atgggatgtc tcaccgcatc gacgtcgagg atgagcaagg ccttgtgctg
      901 gcgacgctgc gattcggcga ggtagtgcgg gtcgaaaatt aggtgagccc atcgcctagt
      961 gccctcgggt gccgaacgct gtcgcgaggt ttagggaggc gggatcgacg agtcgattca
     1021 agaggggtaa gaaggctcac agggcaaaca caccttcgat gttcgcgctt gccgctttat
     1081 gctgctctag ccaagcactt gcggcggagt acctcacctc cgtgaggagt gaggtgtatc
     1141 agactggcgg aaccgcgaag gagatcgcca cgcgcgctgg cacgtgcatc tcgcaacacc
     1201 tggcgccagg cacagtcaat gcacaactca ttatcagcag tgatttagat gggggcgtaa
     1261 tcgtcgctcg cagcgcactc gagtacggcg gcttggtgca gtggaaaatc cggagcaatt
     1321 tcacctttga ggcacgggaa ggtcgcttcc gcatcgtgca gacgaacctt gagcgattca
     1381 acgatcagtt cgatgtcggc tggaagccta tcggcgcatg gaccagctca cagtggaaag
     1441 gagcggagaa ggctttctcg acctcggcca cagtcgtcgc ccaatgcgtc atcaacggac
     1501 tcaagcgcga aacgtggtga gtgcaagccg gtgactaacc ggttactgct tgtaaggggc
     1561 tagcaccgca gcgacggtga cgaatcgatg ccctgctgac cggccaataa gctattggcc
     1621 aaaatataaa agaatggcgc gcccggaagg attcgaacct ccgaccccca gattcgtagt
     1681 ctggtgctct atccagctga gctacgggcg cgcgagaggc tgcgacataa ggggaagctt
     1741 tcgggcgcgc aaccccgccg gcccaaaagc ggcgttgccc cggccgcggg cgccgcatag
     1801 ggaaaggcga tggacgcccg ccgccctttt ccgccgctgg ccgcggccgc cgccctcctc
     1861 gccggctgcg cggccgaagg gccgtttccg tcgttggcgc cgcgcgccgt cgagcgccag
     1921 ctggcgaacg aacagccccc tgccccgccc tcgatcatcc ccagcgatcc cgccaccgcg
     1981 ggccgcatcc gcgatatggt cgccgccgcg cgcgagggcg acggcgcctt ccgctcagcc
     2041 ttggccgccg cgcgcccggc cgtcgccggc gccggcgcgg ccggatcgga aagctgggtg
     2101 gcggcgcagc aggcggtctc gcgcgcccag gcggcgcgcg cgccgaccgt caatgcgctc
     2161 gccgacctcg attcgctggc ggtgcggctg gcggcggcgc ggccggcggc gagccaagcc
     2221 gatatcgccg ccgcggttgc cgcggtcgag accgtgcagg cgatcgccga cgcccaggaa
     2281 gacgcgctcg ccggccttcg cgcccgcctc agcggccgct gagcgccgcc ttgtgggcct
     2341 tggcgagctc cacataatgg gccaccccgg cctgctgccc ggccagctcc tcgggcgtca
     2401 ggtcgcggac atatttggcc ggcctgcccg cccataattg cccggcgggg atgcgcttgc
     2461 ctggggtcag catcgcgccg gcggccagca tggcgccgct ctccaccacg cagccgtcca
     2521 tcacgatcgc gccgaggccg acaaaggcgc ggtcgtggag gatgcagcca tgcaccatcg
     2581 cgagatggcc gatcagcacg tcctcgccga tcaacgtcgg atggccggcc gcatcgcccg
     2641 ccttgggcga atcgacgtgg atcacgctgc cgtcctggat gttggtgcgc gcgcccaccc
     2701 ggatgcggtt gacgtcgccg cgcagcacgc aattgtacca gatgctggcc tccggcccga
     2761 tctcgacatc gccgatcagc tgcgcgcccg gcgcgacgaa ggcggcgggg tcgacccgcg
     2821 gggcccggcc gccgaagctc aggaggtgcg ggccgcccat tcctccctcc cgatcgcata
     2881 aacgatgacg gtgctttcgg cccagtccgg cccctcataa tcgagttcgg ggcggcgggt
     2941 catgccgagc cgctccatca gcccccagct cggcgcgttg ccatcgacgg tgagcgacac
     3001 cacccgctcc gcgccgaacg tcccgaacgc ggcctcgagc gaagcgatcg ccgcctcctt
     3061 ggcatagccc tgcccccaca cgtcctcgcg gaagcgccag ccggcctcat attcgccctc
     3121 gaccgggctg ccggcgtcgt tggcgatctt gagcccgcaa aagcccagca attcgccgtc
     3181 ggccttgcgc tcgaccgccc agaaggtgaa gccgcgttcc tgctcatagg cggcgagccg
     3241 cgccaccacc ccggcaatga agtcgggcgt cttggccccg cccagccagc gcatgacggc
     3301 gggcgtgttg gtgtgacgct cgaagcttgt cgcgtcgccg tcgcgccagc tgcgaagagt
     3361 gagccgctcg gtttcgatca tcgccatcgc ctatccccca cgccctcagt ccgtccagcc
     3421 ttcgatcagc ggcaggatgc tcgcgaaatc gtcactccag ccttcgaagc ccggccgcgg
     3481 ctcgagctcc tcccacggcg cgaacacgcc gatcccgagg aacatgtcgt cgagcgcctc
     3541 cgggctgcgc gccatcgcga tccagaccga ggcggtggcg ttaccctcgt cctcgtcggg
     3601 aaaataatgg agcttcgagg ccgcccagcc gccctgcgcc gccgccgccg cgacgaccgg
     3661 ctccaggtcg agatagcggt tggagatgtg gacgagcagg atgccgcgcg gctgcagcgc
     3721 ccgggcatag acctggaacg cctcgcgggt gaggaggtgc atcggcaccg aatccgagga
     3781 gaaggcgtcg atcgccagca tgtcgagccc cgcggccggc tggcgggcga gcgtcagccg
     3841 ggcatcgccg atcaccatct gcgcctgcgg ggcgcagcta ttgaggaagg tgaaatagcc
     3901 gctttcgcgc gccagccgca ccatcgccgg gtcgatctcg aaaaaccgcc agcgctgccc
     3961 cggccgggca tagcaggcca gcgtgccggt gccgagaccg accacgccga tccgcgcgcg
     4021 ctggccgaac agccgctcgg ccgccccgag cgcaagcccg acgccggact tgcgcgcata
     4081 gtaagtggtc ggctcgagtt cgcggccgcg cgtgatgttc tgaaggccgt ggagggtggt
     4141 gccgtgggtc agcgacaagg cgttgcgatc ggcgtcggtg ccgaccgagt aaatgccgaa
     4201 atagctgcgc gtccgtacgc ccgcatagct tcggtggaca ttgctccagc cgccatagct
     4261 catcatcaag gcggcgaggc aggcggcgaa cacgaccggg cgccccaggc tgaacaaggc
     4321 ggccgcgccg atcgcgccgc tcgccgccac cgccacccag accggcgcct cgaacaggaa
     4381 gcgccggtcg ccgacgagcg acagcaggaa caccgccacg ggcatcgcca tcgccagcac
     4441 gaacccgcgc ttcggctcgc tccagatccg ctcgatccgc ttcagataat tgcgctgcgg
     4501 cagcagcagc gccgcggcga ggatcagcag cggatgctcg tagctccagt cgaacagcaa
     4561 cggcgcggcg agcgcgcaga aggccccgcc gaggacgccg cccaccgaca tggcgaggta
     4621 gaagccggtc agccggtcgg gctccggccg cagccggtac atttccgcat ggagcgcgac
     4681 cgcgacgaag aacagcaggc tcagccccag cgccgccgcg gccagcgggt tgcgcgatcc
     4741 gtcggcgaag gcgagcccgc ccgcgatcag cagcaccaaa ggcgcgacct ggccgatgaa
     4801 ctcggcggcg ccgcgccgcc gcgcgaaggc gatgacgaag ctcaacagat aaaggccgag
     4861 cggcagcgcc cacagcatcg gcatggcgac gatgtcggtg gtgaggtggg tcgtggtcga
     4921 cagcatgagc cccgacggca ccgccgccag cgcaatccac agcccgaccc gccgccggct
     4981 cggcgccggc gaggccggct ccggcaccgc ctcgaccgcc tgccccggta tggtgagcgc
     5041 gcagccgacc accagcagca ccagcaaagc atagccgccg gtccagagcg cgctttgctg
     5101 gtcgagcgtc agcagcggct cgaccagcaa agggtaggaa atgagccccg cgaagctgcc
     5161 gaggttcgag gcggcgtaga gcgcataagg ctcgccgcgc gcgctttcga gcgcatacca
     5221 gcgctgcatc agcggcgcct gcgaggagac gatgaagaat agcggcccga tcgagctcaa
     5281 tagcagccac ggcgtccaca aagccggcga cacgcccccc ggcggcaaag cggcgccgag
     5341 cccgatcgga agccacaaag cggcgatggc gaacaggccg agatgcagcc ccgcctgcgt
     5401 ccgcggccgc agccgcgcca actggtgggc ataggcatag ccgccgagca gcagcgcctg
     5461 gtagaccatc atcgcgctgt tccacaccgc cggcgcgccc ccgagccgcg gcagcgccat
     5521 ccgcgcgatc atcggctggg tcaggaacag caggaacgat ccggtgacga tggtcgcgaa
     5581 gaagagcggc cgtgcatagg cctcgcgtgc ggaaggcgcg gcccccgcct cagcggtcac
     5641 ggagcagccg cgccgccacc ggcgcgtgat agctgagcac gcccgcgcag ccggcccgct
     5701 tgaacgccgt cagcgtctcc atcagcagcg cgtcgcggtc gcccgccccg gccgccgccg
     5761 cggcctcgat catcgcatat tcgcccgaca cctgataggc gaagaccggc acttcgaagc
     5821 gctccttcac ccgccggacg atgtcgagat aggcgaggcc cggcttcacc atgacatgat
     5881 cggcgccctc ggcgatatcg agcgcgacct cgcgcagcgc ctcgatcgcg ttggcgggat
     5941 ccatctggta gctcttcttg tcgcccttga gccgcccggc cgacccgacc gcgtcacgga
     6001 acggcccgta aaaggcgctc gcatatttgg cggcataggc catgatctgg acgttgacgt
     6061 ggccctcgcg ctcgagcgcc tggcggattg cgccgacccg cccgtccatc atgtccgacg
     6121 gggcgacgat gtccgccccc gccgccgcct gcaccagcgc ctgctcgacc agcatcttgc
     6181 tggtctcgtc gttcagcaca tagcccgcct cgtcggcgat gccgtcatgg ccgtcggccg
     6241 tataagggtc gagcgcgacg tcggtgagga tgcccacgtc cggcgccgcg tccttggccg
     6301 ccttgatcgc gcggcacatc agattgtcgg ggttgagcgc ctcctgcccg ccctcggtcc
     6361 ggagctcggc gggcgtgttc ggaaacaaag cgatgcacgg aatgccgagc cgctgcgcct
     6421 cgcgcgcgcg ctcggggatg aagtcgatcg accagcgcga cacgcccggc agggtcgcga
     6481 tcggctcctc cacggcgtgg ccgccggtca cgaacaacgg ccagatcagg tccgacggcg
     6541 tcagcaccgt ctcggccacc atcgcccgcg accaggcggc ggagcgggtg cggcgaagcc
     6601 ggacggcggg aaaggaggcg gcggccatgg ccggctagta gcgggcgccg ccccgcctgc
     6661 aaagccgcca catggccgcg tcccacgccc cgaatcccgg ctcagcgcgg gcgccacccc
     6721 cctccggttg tgcgccgacc gctttgatgt cattctccgc cggtaagctt ccccgcaaga
     6781 cgggaccgaa cacgcctgca cccttcactc ccacatggaa ggacggccga tgaagctcct
     6841 gaaacccctc gcaaccctgt ccgccggcgc gatgctggcg gcgatcgcgg ccagcgccgc
     6901 cgcccagccc gtggccccgc ccccggccgc cccgccgccg cttccccagc tctatttgct
     6961 gggccaggaa ccctattcgg tcagcggccg caatttcgtg cgctaccgct acgacgtgca
     7021 caataaggac aaatacccgg ccgagctgtt cgcagccgcg ccgaacctgc cgccgtgcgg
     7081 cctcaacgcc aattcctcgc gcacctgggt ggacatcgtc gagcagggcg ggcggcgcat
     7141 ctacggcttt tgcgcgctgg gcgatccgca atatctcggc cagatctggt tcgcgatgga
     7201 ggaaggcacg atcccgccga gctggatcta tatcgagatg accgatcgcc agaccaacac
     7261 taaataccgg tccaatctcg ccgacaccac gctttagcgc ccggcgagga cgagccgcgc
     7321 ggccggccgc tggcgccgat ccgcggcggg cgggacgatg gacaccctgg cccaccggtg
     7381 agttagtccg cggtgagcct gatcaaaccc gtccccaagg aagccgtcct cgtcgtcaac
     7441 gcgcattcgc gcaagggcga ggaactgttt cgcgaggcca ccgccaagct cgaaatgtcc
     7501 ggcgtccggc tgatcgaatc ctatcccgtc cgcgatcccg acgagctcat cccgacggtg
     7561 cgaaaggcgg tggcggcggg ggcgccgatg gtgatcgtcg gcggcggcga cggctcgctc
     7621 tcgtccacgg tcgacgatgt ggtcggccgc gactgcgtgt tcgctttgct gccgctcggc
     7681 accgccaaca gcttcgcccg caccctcggc atcccgctcg accttgacgg agcgatcgcc
     7741 accatcgcca ccggcaagcg caggcgcatc gacctcggcg tcatcaacgg cgactatttc
     7801 gccaatgcgg ccgccatcgg cctgtcgccg atgatcggcg atacggtgcc gcacgagctc
     7861 aagaaatatc tcggccgctt cggctatctg ctgtgggcgg tctggtgctt cgcgaggttc
     7921 ggcccgttcc ggctgttcgt cgacgacggc accaaggtgc atcggctgtg gacgaccgag
     7981 gtccgcatct tcaacggccg tttccatggc ggggtcgagc tgaccgagac caccgacgtc
     8041 gacagcggcg acatcgtcat ccaggcggtc accgggcgcg gcctcgcgcg gctggtgtgg
     8101 gattggtacg ccaaattctt caagctgagc tcgcgcgacg ccaacacggt cgagttccgc
     8161 ggcaagagcc ttcacgtcga cacccggccg caccaccgca tctcaatcga cggcgagatc
     8221 gtcgcccgca cccccgccac ggtcgccatc gcgagccgcg cgatcgaagt ggtagtcccc
     8281 gcctaaccga gccgcgccag cgccgcccgg tacttttcgg cgtcggaggc tttttcggca
     8341 tggtcggacc gcgccttttc gacagcctcg ggcttggccc gctcgacgaa gctcggattg
     8401 ccgagccgcg cggccagcgc atcgcgctcc ttctccgccg cctgcagcgc cttggcgagc
     8461 cgcgcccgct cggccgcgat gtcgatcacg ccctccagcg gcagcacgaa cgtcgcctcg
     8521 tcgacgacca gctgggcggc ccctccggcc ggcgcctcgc cttccgccac cgcctcgatc
     8581 cgcgccagcc gccggattgc cccgtcgttg cgccgcagcc gctccagcgt ctgcaccccg
     8641 gcgtccttga catggagcgc aagcttcgcc cccggcggca cgccgagctc ggtgcgggcg
     8701 gcgcggatgc cgccgatcag ccggatcagc caatccacct ccggccccgc ctccggatcg
     8761 agcctgcgcg cgtccggcat cggccatttg gcgacgatca ggtcgtaagg ccgctcgccc
     8821 atcgcattcc aaagctcttc ggtgatgaac ggcatgaacg ggtgcagcat caccaatatc
     8881 tggtcgagca cccagccggc gaccgcccgc gtctcctcgt ccacgcggcc tcgctcgtcg
     8941 ccgctcgata ccggcttgat cagctccaga taccagtcgc agaaagtcgc ccaggtgaac
     9001 tgatagataa ggttcgcgct ttcgtcgaag cgcagctcgg ccatggcgag gtcgagcgcc
     9061 tggaccgtct tcacggtctc gccgacgatc cacttgttga cgggaagctc cgccgcgggc
     9121 ggctccagtc cggccgacgc cccgatcccg ttggcctggc agaaacgcgc cgcattccat
     9181 agcttggtcg cgaagttgcg atagccctcg acgcgcttct catccaattt gatgtcgcgc
     9241 ccctggctct ccatcgccgc cagcgtgaac cgcaaggcgt cggcgccgta gcggtcgacg
     9301 agccccagcg gatcgaccgt attgcccttc gacttggaca ttttcgcgcc ctgc
//
LOCUS       DASVRK010000068         4717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_446901, whole genome shotgun sequence.
ACCESSION   DASVRK010000068 DASVRK010000000
VERSION     DASVRK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446901"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VF589_02360"
     CDS             <1..571
                     /locus_tag="VF589_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX8256452.1"
                     /translation="GDSAAAGGGEAIDRPWKRGSAEDAAFLMKQAEQVIIVPGYGMAV
                     AQAQHALREMGDLLKAEGVSVKYAIHPVAGRMPGHMNVLLAEANVSYDEVFELEDINS
                     EFARTDVAFVIGANDVTNPAAKTDKSSPIYGMPVLDVEKAKTVLFIKRSMGGAGYAGV
                     ENELFFRDNTMMLLADAKKMVEEIVKNLG"
     gene            644..1387
                     /locus_tag="VF589_02365"
     CDS             644..1387
                     /locus_tag="VF589_02365"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX8256453.1"
                     /translation="MRKLGLIGGMSWVSTAMYYEQINKSVNKRLGGLASAPLLIESLD
                     FAPVAAMQAAGEWDALAELMIATARRLQDGGAEGLLLSSNTMHKLYDRIAAEVSVPLI
                     HIGEVTAAKLVKDGVKRAGLIGTRFTMGETFYRERLEASGIAVTTPGAAMAREIDRII
                     FDELTRGHVSRISQRTLKTCLTEMGQARQQAVILGCTELVMLVDPVANVLPVYDTTAL
                     HAAAAVKWILEEPAAAAPAEPALSEAGAA"
     gene            1543..2487
                     /locus_tag="VF589_02370"
     CDS             1543..2487
                     /locus_tag="VF589_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX8256454.1"
                     /translation="MAMPQGKSHDVPAMTEYGDHPSVMILADGEPAAERARASAERAG
                     RRVSAIAPVVGGLERLARQVAVGAVLVEVDGAGEGENLAELERLLGGLDAAAEAGRYG
                     SVVAAPPELIDFIAARAPHRDVFHLCGASEVERVLAIALASARRPLRLHDGRRPPGTD
                     KLQQLGEEVGRIASLLASLSEEVAGGGCGAGAEEGAPRSELTAGRVRAIIRGRRLRDQ
                     FFRPDFFADPAWDMLLDLMAARLEKQRVAVSSLCIAAAVPPTTALRWIKTLTDQGLFV
                     RQADPQDGRRVYIELSDEAAAALEAYLSAVQRISPLSI"
     gene            complement(2536..2611)
                     /locus_tag="VF589_02375"
     tRNA            complement(2536..2611)
                     /locus_tag="VF589_02375"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2576..2578),aa:Val,seq:tac)
     gene            complement(3005..3568)
                     /locus_tag="VF589_02380"
     CDS             complement(3005..3568)
                     /locus_tag="VF589_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8256455.1"
                     /translation="MITRIRDVRRAKRMTLQDVADRCEPPTTPQTIGRLETGTRTVSV
                     GWLNRIAKALGVEASDLVTLPDREDVPVAATLAHDGAHAPRRPGVAIPPQAEPGLVAI
                     RVEASVGEYRAGDEIWASRVGPEAFAQAMNRDVLLPRPAGRFVFGRLIGREEGKLHIL
                     PLGAGQRQTVVTDPAWAAVATRLIRNL"
     gene            3692..4180
                     /locus_tag="VF589_02385"
     CDS             3692..4180
                     /locus_tag="VF589_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEX8256456.1"
                     /translation="MAERPLPRDGQERPRPGGPKPGARGANARSVTVNLAESPLGWLR
                     SRGLVSERQLQAGERLREDWERAQLSPRVTMQWDAPPLAKTRRGPPVNIASDAQIAAK
                     RRFEVAMAEVGPGMSDVMWRIVCAGEGMRDAERALGWPARAGKLVLGLALDRLADHYR
                     LP"
     gene            complement(4219..4512)
                     /locus_tag="VF589_02390"
     CDS             complement(4219..4512)
                     /locus_tag="VF589_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256457.1"
                     /translation="MLHTKTQDRNSANPHGEESCSHDLKARGYSQVYRENQVNNCPGC
                     GHTQWYVGRISAECGYCATALPLQDASARGGAAIHTRNNRPVFFHHAGRSLTG"
BASE COUNT          740 a         1516 c         1747 g          714 t
ORIGIN      
        1 cggcgacagc gcggcagccg gtggcgggga ggcgatcgac cgcccctgga agcgcggctc
       61 ggccgaggac gcagccttcc tcatgaagca ggccgagcag gtcatcatcg tccccggtta
      121 cggcatggcg gtcgcgcagg cccagcatgc gctcagggaa atgggcgacc tcctcaaggc
      181 cgagggcgtc agcgtcaaat atgcgatcca cccggtcgcc ggccgcatgc ccggccacat
      241 gaacgtgctc ctcgccgagg ccaacgtctc ctatgacgag gtgttcgagc tcgaggacat
      301 caactccgaa ttcgcccgca ccgatgtcgc cttcgtgatc ggcgccaacg acgtcaccaa
      361 cccagccgcg aaaaccgaca agtcgtcgcc tatctatggc atgccggtgc tcgatgtgga
      421 aaaagccaag acggtgctgt tcatcaagcg ctcgatgggc ggcgccggct atgccggtgt
      481 ggagaacgag ctcttcttcc gcgacaacac gatgatgctg ctcgcggacg cgaagaagat
      541 ggtcgaggag atcgtgaaga atctgggcta gccagatcgc gcggatcccc ggccctcagg
      601 ctcgaacggg ggccgccggc gattgggcga tgggacaggc gacgtgcgta aattgggttt
      661 gatcggcggg atgagctggg tctcgaccgc catgtattac gagcagatca acaaaagcgt
      721 gaacaagcgg ctcggcggcc tcgcctcggc gccgctgctg atcgagagcc tcgacttcgc
      781 gccggtggcg gcgatgcagg cggcgggcga gtgggacgct ttggccgagc tgatgattgc
      841 gaccgcgcgc cggctccagg atggcggcgc cgaggggctg ctgctatcga gcaacaccat
      901 gcacaagctc tacgaccgga tcgcggccga agtgtcggtc ccgctcatcc atatcggcga
      961 ggtgacggcg gcgaagctcg tcaaggacgg cgtcaagcgg gccgggctga tcggcacccg
     1021 cttcacgatg ggcgagactt tctatcgcga acggctcgag gcgagcggta ttgcggtgac
     1081 gacgcccggc gccgccatgg cgcgcgagat cgaccgcatc atcttcgacg aactcacccg
     1141 cggccatgtc tcgcgcatct cgcagcggac gctcaagacc tgcctcaccg agatgggcca
     1201 ggcgcgccag caggcggtga tcctcggctg caccgagctg gtgatgctgg tggatccggt
     1261 cgccaacgtg cttccggtct acgacacgac cgcgctccat gccgcggccg cggtcaagtg
     1321 gatcctcgaa gagccggcgg ccgctgcgcc ggcggagccg gccttgtcgg aagcgggcgc
     1381 ggcctaggca ccgcccaagc gcgggaagtt agctccggtt cggatgaaac gggcgctgcg
     1441 gggccgctcc cgtttcggtc cgaaatggag gagtcggccg ctcctttttc atccgcaatg
     1501 gcgtcaaagt ggaagaacct ttattcctca gggggggaag ccatggccat gccgcagggg
     1561 aagtcgcatg atgtgccggc gatgacggaa tatggcgatc acccatcggt gatgatcctg
     1621 gccgatggcg agccggcggc cgagcgcgcg cgggcttcgg ccgagcgggc cgggcgccgg
     1681 gtgagcgcga tcgctccggt cgtgggcggg cttgaacggc tcgcccgcca ggtcgctgtc
     1741 ggtgcggtgc tggtggaggt cgatggcgcg ggtgagggcg agaatctggc cgagctcgaa
     1801 cgcttgctgg gcgggctcga cgccgccgcc gaagcggggc gctatggcag cgtggttgcg
     1861 gctccgcccg agctgatcga cttcatcgcg gcgcgggcgc cgcatcgcga tgtcttccat
     1921 ttgtgcggcg cctccgaggt cgagcgagtg ctcgccatcg cgctcgcttc ggcgcgccgg
     1981 ccgctgcggc tccatgatgg gcgccgcccg ccgggcaccg acaagctgca gcagctgggc
     2041 gaggaggtgg gccggatcgc cagcctgctc gccagcctct ccgaggaggt ggcgggcggc
     2101 ggctgcggcg ccggggccga ggagggcgcg cccaggtccg agctcaccgc cgggagagtc
     2161 cgcgccatca tccgcggccg ccgcctgcgc gaccagtttt tcaggcccga cttctttgcc
     2221 gatcccgcct gggacatgct gctcgacctg atggcggccc ggctcgagaa gcagcgggtg
     2281 gcggtatcga gcctgtgcat cgccgccgcc gtgccgccga ccaccgcgct gcgctggatc
     2341 aagacgctga ccgaccaggg cctgttcgtg cgccaggccg acccgcagga cgggcgccgc
     2401 gtctatatcg aattgtccga cgaggcggcg gcggcgttgg aagcctatct gagcgccgtc
     2461 cagcgcatct cgccgttgtc gatctgaacg gcgctgtcga tctgaagggt gggccggccg
     2521 ctgcttcggc cgggctggtg ggcgctgacg ggctcgaacc gccgaccctc tcggtgtaaa
     2581 cgagatgctc taccaactga gctaagcgcc cgcgccaggc ggttcagcgc ccctgccatg
     2641 ggcgggcggc gcgggcaagc ctcagagcca gccgagcacc gccagcagga ccagagtggc
     2701 cagcaccgag atcaggaccg agccgaggca acccaggcga ttggagaaaa agaagaacaa
     2761 ggcgatgctc ccgagctgat ccggacacaa cgcagcagcc ggggttcgtt cccgcgcggc
     2821 gatgaggggg gagggtgcac gtgaaaatcc ccgctcatcc tgaggagccg ctgagcgaag
     2881 tcgaagcggc gtctcgaagg acgcacggtg gtccttcgag acgggccctc gccggggctc
     2941 attccctcct caggatgagc gggaatgctt tacccagctg atcacgctcg ccggtccgct
     3001 ccccctacag gttccggatc agccgtgtag cgacggcggc ccaggctggg tcggtcacca
     3061 cggtctggcg ctggccggcg ccgagcggga ggatgtggag tttgccctcc tcgcggccga
     3121 tcagccggcc gaacacgaag cggccggcgg ggcgcggcag caggacgtcg cggttcatcg
     3181 cctgcgcgaa ggcctcgggc ccgactcggg acgcccagat ttcgtcgccg gcgcgatatt
     3241 cgccgacgct ggcctcgacc cggatggcga ccagcccggg ctcggcctgc ggcgggatcg
     3301 cgacgcctgg ccggcgcggc gcgtgggcgc cgtcatgggc gagcgtcgcg gcgacgggga
     3361 cgtcctcgcg gtcgggcagg gtgacgagat cggacgcttc cacgccgagc gccttggcga
     3421 tgcggttcag ccagccgacg gagacggtgc gggtgccggt ctcgagccgg ccgatcgtct
     3481 gcggggtggt gggcggctcg cagcggtcgg ccacatcctg cagggtcatc cgcttggcgc
     3541 ggcggacgtc gcggatacga gtgatcatgc cgaaagaatc ctgtaccacg tgggttattt
     3601 cactttccta caggcaatcc ccaatggcaa gacatttctc cagagggcga ctcggggaag
     3661 ggcgatgggt gcgttgagga ggaaccggct gttggcggag cggccgctgc cgagggacgg
     3721 gcaggaacgc ccccgacccg gcggtcccaa gccaggcgcg cgcggcgcca atgcccgctc
     3781 ggtcaccgtc aacctcgcgg aatcgccgct cggctggctg aggtcgcggg ggctggtcag
     3841 cgagcggcag ctgcaggccg gcgagcggct gcgcgaggat tgggagcggg cgcagctcag
     3901 cccgcgcgtg accatgcaat gggacgcgcc gccgcttgcc aagacgcggc gcgggccccc
     3961 ggtgaacatc gcgagcgacg cccagatcgc tgccaaacgg cgcttcgagg tcgcgatggc
     4021 cgaagtgggg ccggggatga gcgacgtgat gtggcggatc gtgtgcgcgg gcgaaggcat
     4081 gcgcgacgcc gagcgcgcgc ttggctggcc ggcgcgggcg ggcaaattgg tgctcggcct
     4141 ggcgctcgac cggctggccg atcattatcg cctgccgtga acgggcggcg gcggagtcgc
     4201 ccagccgcaa gcgaaggctc agccggtcag cgaacggccg gcgtggtgaa agaagaccgg
     4261 gcggttgttg cgggtgtgga tcgccgcgcc gccgcgcgcg ctcgcgtcct ggagcggcaa
     4321 ggcggtggcg caatagccgc attccgccga aatccgtccg acataccatt gggtgtgccc
     4381 gcagccgggg cagttgttga cctggttctc gcgatagacc tggctgtagc cgcgcgcctt
     4441 gaggtcgtgc gagcagctct cctccccatg cgggttcgcg gagttccggt cttgggtctt
     4501 ggtatgcagc atggtcgttc cccctgccga gtcgagatag aacgcgcgag cagcagttcg
     4561 tatccggtgc ccgcgcgatg ctccgcgggg cgatctaaca ggcatgggga cggccggcgc
     4621 cgggcgcatg ttcctgctca tgggcggtcc gtgtgggcca gcctcgggag gaaattacct
     4681 ggtccgagcg gcctgggaga atgtttatgc gggatcg
//
LOCUS       DASVRK010000069         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_322604, whole genome shotgun sequence.
ACCESSION   DASVRK010000069 DASVRK010000000
VERSION     DASVRK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322604"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF589_02395"
     CDS             complement(<1..201)
                     /locus_tag="VF589_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HEX8256458.1"
                     /translation="MRVSAKSKIAFAIGSGAVALAGCASGGAPTGNRLPTASFVSGTE
                     QVSEEYIIGAMDELTVFVWRNPE"
     gene            complement(366..1604)
                     /locus_tag="VF589_02400"
     CDS             complement(366..1604)
                     /locus_tag="VF589_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HEX8256459.1"
                     /translation="MSKSMGAIPADFAAEGAALMIGGRSAEALAAQAGDTPLFVYDFG
                     IVRGRIARFRSAFPRVKLHYAVKANPYQPLLRSIAKQVDGLDVASAGELRLAVEAGAD
                     AASISFAGPGKRDRELEAAIAAGATLNCESEGEAERALRIGERLSLRPRLAVRVNPDF
                     EIKGSAMRMGGGAKPFGVDAERVPALVRTILGAGADWRGFHIFAGSQALSAEALIEAQ
                     RATLALAGALADAVGAPPPTVNLGGGFGIPYFTGEQPLDIERVGAALAEALDSRASSL
                     ANSAFAIELGRWLVGEAGVYLTRIVDRKVSHGKTFLIVDGGLHHQLAASGNFGQLVRR
                     NYPVAIATLYGEPPVEEASIVGCLCTPLDRLADDVMVPRAGVGDLVAVFQAGAYGLSA
                     SPQAFLGQPAALEMLVGGDD"
     gene            complement(1601..3118)
                     /locus_tag="VF589_02405"
     CDS             complement(1601..3118)
                     /locus_tag="VF589_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HEX8256460.1"
                     /translation="MQAPDPNPRPLDHLTLRGAAAAPALITREGVLDYGGLERHVAAL
                     AAALRAKGLGQGDRVASWLPKTRMTSLLPLACARAGLVHVPLNPLLKRAQVAHILADS
                     GAALLISGKARLGTLGEGDLNCPAVEEEEGAAMLAGADGLPPSSADPDDLAALLYTSG
                     STGRPKGVMLSHANLWLGAVSVAYYLDLTPADTVLGVLPLSFDYGQNQLLSSWAAGAC
                     AVPIEYLTARDVIRAMERHDVTTLAGVPPLWVQLIEAAWPPSALLRLRRLTNSGGKLP
                     SSIVRRMREMFPGAELYLMYGLTEAFRSTFLNPALVDEFPDSIGTAIPFAEVMVARPD
                     GSEAGPGEPGELVHAGPLVARGYWNDPARTAERFRPAPSWSHYGGTAVWSGDTVRRDE
                     HGLLTFVARDDAMIKTSGNRVSPTEVEEAAVASGLVAEAVALGFPDHRLGQAIALVVR
                     PQPPRGKEAEADLRNFLKRELPNFMQPSAIVWRAELPKGPNGKIDREGLKSELMG"
BASE COUNT          511 a         1074 c         1123 g          452 t
ORIGIN      
        1 ctccgggttg cgccacacga acacggtcag ctcgtccatc gcgccgatga tatattcctc
       61 gctgacctgc tccgtgccgc tgacgaagga agcggtcggg agccggttgc cggtgggagc
      121 tccgcccgag gcgcagccgg ccagcgcaac cgcgcccgaa ccgatggcaa aagcgatctt
      181 ggacttggca gaaacacgca tggatcgtcc ctcttggtca gctttcccat ccgccccgcc
      241 cgtccagaac agggacggga cagacgcaga tttggctctg ctatgatctg aagaggtaaa
      301 attacggtta ggaacaagcc gctttccggg gccgtcccgc tttgggaccg ccgcctgggg
      361 cagggttaat cgtcgccgcc gaccagcatt tccagcgccg cgggctggcc gagaaaggcc
      421 tgcgggctgg cgctgaggcc gtaggcgccg gcctggaaga cggcgacgag gtcgccgacg
      481 ccggcgcgtg gcaccatgac gtcatcggcg agccggtcga gcggggtgca caggcagccg
      541 acgatgctgg cctcctcgac gggcggctcg ccgtagaggg tggcgatcgc gaccggataa
      601 ttgcggcgga cgagctggcc gaaattgccg ctggcggcga gctggtggtg gaggccgccg
      661 tcgacgatca ggaaggtctt gccgtggctc accttgcggt cgacgatgcg ggtgagatag
      721 acccccgcct ccccgaccag ccagcggccg agctcgatcg cgaaggcgct gttcgcgagc
      781 gaagacgcgc ggctgtccag ggcctcggcg agggctgcgc cgacccgctc gatgtcgagc
      841 ggttgctcgc cggtgaaata agggatgccg aagccgccgc ccaaattgac cgtaggcggc
      901 ggcgcgccga ccgcatcggc gagcgcgccg gccaaggcga gcgtcgcccg ctgcgcctcg
      961 atcagcgcct cggcggaaag cgcctgcgag ccggcgaaga tgtggaagcc gcgccagtcg
     1021 gcgccggcgc cgaggatggt gcgcaccaaa gccggcacgc gctcggcatc gacgccgaac
     1081 ggcttggctc cgccgcccat ccgcattgcc gagcccttga tctcgaaatc gggattgact
     1141 cgcacggcga ggcgcgggcg cagcgacagc cgctcgccga tgcgcagcgc ccgttcggcc
     1201 tcgccctccg attcgcagtt gagcgtcgcg ccggccgcga tcgccgcctc gagctcgcgg
     1261 tcgcgcttgc cggggccggc gaaggaaatg gatgcggcat ccgcaccggc ctcgaccgcg
     1321 aggcgaagct cgcccgccga ggccacgtcg aggccgtcaa cctgcttcgc aatcgacctt
     1381 agaagtggtt gataagggtt tgccttcacg gcataatgca gcttaaccct cggaaaggcc
     1441 gagcggaagc gggcgatccg cccgcggacg atgccgaaat cgtagacgaa caagggcgtg
     1501 tcgcccgctt gcgccgcgag cgcctcggcc gagcggcccc cgatcatgag cgcggcgccc
     1561 tcggcggcga aatccgccgg tatcgcgccc atgcttttgc tcatcccatc agctcgcttt
     1621 tcagtccttc gcggtcgatc ttgccgttgg ggcccttggg cagctcggcg cgccagacga
     1681 tagccgaggg ctgcataaaa ttgggtaact cgcgcttcag aaagtttctg aggtcggctt
     1741 cggcctcctt gccgcgcggc ggctgcgggc ggacgaccag cgcgatcgcc tggcccagcc
     1801 ggtgatcggg aaagccgagc gccaccgctt cggcgacgag cccgctcgcc accgccgcct
     1861 cctcgacctc ggtcgggctc acccggttgc ccgacgtctt gatcatggca tcgtcgcggg
     1921 cgacgaaggt gagcaggccg tgctcgtccc gccgcaccgt gtcgccggac cagacggcgg
     1981 tgccgccgta atgagaccag gacggcgccg gccggaagcg ctcggcggtg cgcgcggggt
     2041 cgttccaata gccgcgggcg acgagcggcc cggcatggac gagttcgccc ggctcccccg
     2101 gaccggcctc ggatccgtcg ggccgcgcca ccatcacttc ggcgaagggg atggcggtgc
     2161 cgatcgaatc gggaaattcg tcgaccaaag cgggattgag gaaggtcgag cggaacgctt
     2221 cggtgaggcc gtacatcaga tagagctcgg cccccgggaa catctcgcgc atccgccgca
     2281 cgatcgagct cggcagcttg ccgcccgaat tggtgagccg gcggaggcgg agcagcgccg
     2341 agggcggcca agcggcctcg atcagctgca cccacagcgg cggcacgccg gccagcgtgg
     2401 tgacgtcatg acgctccatc gcgcgaatca cgtcgcgtgc ggtgagatat tcgatcggca
     2461 ccgcgcaggc gcccgcagcc cagctcgaaa gcagctggtt ctggccataa tcgaagctga
     2521 gcggaagcac gccgagaacg gtgtccgcgg gcgtgagatc gagataataa gcgacgctga
     2581 ccgcgccgag ccacagattg gcgtggctca gcatcacgcc cttcggccgt ccggtcgagc
     2641 ccgacgtata gagcaaggcg gcaagatcgt ccggatccgc gctggaaggc ggcaacccgt
     2701 cggcaccggc cagcatcgcc gccccctctt cctcctcgac ggccgggcag ttgagatcgc
     2761 cctccccgag cgtccccagc cgcgccttgc cgctgatcaa gagggcggcg ccgctgtcgg
     2821 cgaggatgtg ggcgacctgg gcgcgcttga gcaacgggtt gagcggcacg tggacgaggc
     2881 cggcgcgggc gcaggcgagc ggcaacaggc tggtcatgcg tgtcttggga agccagctcg
     2941 ccaccctatc cccctgcccc agccctttgg cgcggagcgc tgcggcgagg gccgcgacat
     3001 gacgctccag cccgccataa tcgagcacgc cctcgcgggt gatcagcgcc ggcgcggccg
     3061 cggcgccgcg caaggtcagg tggtcgagcg ggcgggggtt tggatcgggg gcttgcacct
     3121 tggctcctgg ctgcgccact acggcgggag ggggatagcg
//
LOCUS       DASVRK010000070        12000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_447236, whole genome shotgun sequence.
ACCESSION   DASVRK010000070 DASVRK010000000
VERSION     DASVRK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447236"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VF589_02410"
     CDS             <1..994
                     /locus_tag="VF589_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8256461.1"
                     /translation="DPELIAAGSYWTARADMMCGRPERVQARLRTAARLKETFYGLLA
                     AKALGIKTGNFAGLHDFRDAEWRSIAGKPNVKAAIALTEIGEAGLAEEHIKHQARIGS
                     RRDHDALLHLASDLNLPGTQYWLAHNAPPGSYVNLAARYPAPDWRPSRGWRVDKSLAF
                     AHALQESSFRTSVVSPAGARGLMQVRPGTAGDIAKKRGESFDPSHLNDPAANLEYGQS
                     YLEYLRSHHGTGGLLPKVIAAYNAGPNPIAEWNYRRMDQGDPLLYIESIPYWETRGYV
                     PIVLRNYWIYEQQEGRDSASRHALAEGKWPRFPGLPGATAVRIGPAGGASGGTN"
     gene            1037..2113
                     /locus_tag="VF589_02415"
     CDS             1037..2113
                     /locus_tag="VF589_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX8256462.1"
                     /translation="MHRPDARAGRGAPENRTPTRFNLKEREADGDWLDAREALGEGAP
                     PLRTSVTIETPKTIITRNLSPDVGFDRSINAYRGCEHGCIYCFARPTHAFHDLSPGLD
                     FESKLFAKPDAAKLLREELGKRGYVVRPIAMGTNTDPYQPIEAEYRITRACIEVLLET
                     RHPLTITTKSDRVLRDLDLLAPMAELGLVSVALSITSLTPEISRTLEPRAPAARKRLA
                     AVKRLGEAGVPAWVAIAPVVPGITDHELEAIVEAAAEAGAKGAFYLPVRLPHEVAPLF
                     RAWLDEHYPERAAKVMHIIQAMRGGKDNDPNWFTRMQGFGPWAELLRTRFEKAARKHG
                     LSGVKNSLRTDLFRPPQGPQLRLF"
     gene            complement(2303..3904)
                     /locus_tag="VF589_02420"
     CDS             complement(2303..3904)
                     /locus_tag="VF589_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8256463.1"
                     /translation="MIGGMQDWPLRIMRLLDHAGREHGPREIVSLYASGERIRTNYAG
                     IARDARRLANALERLGVRRGDRVATLAMNHGRHLVAWYGAIGMGAILHTINPRLFDDQ
                     LVYIANHAEDRVLFYDRAFAPIVERLKPQLGTIEHYVCFDPAEGESGFEDLIASESDD
                     YRWVEGDEREPAMLCYTSGTTGNPKGVLYEHRSTVLHAMAEVSPDIFDLSARAVALPI
                     VPMFHAAAWGLPFAAPLAGVKLVFSADYTPSVMCDLMREEGVTHTAGVPTVWLAVINH
                     VEATGADLGKLEVVTIGGSAAPRSMIRYFVERGIRVGHAWGMTETSPIGTTGAAPAHW
                     DELGQDERIDYMVKQGRVPFGVELRTVADDGTVLPRDGKTSGRLQARGPWVVQRYFKD
                     ASGPAVDEDNWFDTGDVAILHPDGVMQITDRSKDVIKSGGEWISSIELENAAMGCEGV
                     AEAAAVGVHHPKWDERPLLLVVRKPGSDVSPAEITRHLTQHVARWWLPDEILFVETLP
                     HTATGKLLKTALREQYKHHKLPTAA"
     gene            complement(4046..7627)
                     /gene="dnaE"
                     /locus_tag="VF589_02425"
     CDS             complement(4046..7627)
                     /gene="dnaE"
                     /locus_tag="VF589_02425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX8256464.1"
                     /translation="MAAPYVPLRIFSCYTMLEGAIEPKAIAKQAKKLEFPAVALTDRN
                     GLYAAMPFTDACKAEGVQPIIGTMLGLARPGAAGARGAQIDWLALYAQDEAGYQNLCA
                     LVSTAHLGRPLEEEAHVGFDALEGRTDGLIALTGGGEGALARLLAEEQNAAALAVLDR
                     LQALFPGRLYVELCRRGDEVEQRAEPALIDLAYERDLPLVATNPACFTDPAFHRAHDA
                     MLCIAQSSQIDRDDRAKSSPEAWIKPAADMRKLFEDLPEAIANTSVVARRCAFGAPKR
                     SPILPRIAGDLEAEAEQLRRDARAGLEARLADYQLPGEGRGPGMPDPDAALTGGALET
                     RYKAYFDRLDYEVEIIVGMGFAAYFLIVADFIKWAKEHDIPVGPGRGSGAGSVVAWSL
                     TITDLDPIRLGLLFERFLNPERVSMPDFDIDFCETRRGDVIRYVQQRYGADKVAQIIT
                     FGKLKARAVLKDTGRVLQMPYGQVDRLAKLIPNHPTEPWDLERALNGVSELATEYKND
                     AQVRRLFDLARKLEGLPRHSSTHAAGVVIGDRPLDKLVPLYRDPRSDMPVTQFDMKYV
                     EAAGLVKFDFLGLKTLSVLQKAVQMLAKRGITVDLDRIPWDDAEVFKLLQRGETVGVF
                     QLESEGMRRTLAAVKPTGFEDIIALVSLYRPGPMDNIPMFGARKNGREPIEYPHPMLE
                     NVLKETYGIFVYQEQVMEAAKVLAGYSLGEADLLRRAMGKKIKAEMDAQRERFVSGCG
                     ANQIGRPKANELFDLIDKFAGYGFNKSHAAAYALVAYQTAWLKAHHPVEFYAASMCFD
                     MAQTDKLGIFVEDMRRLEVACLPPSINASEAEFSVELLPSVPAKAGTQTGLLPSQEHR
                     LAVRYALGALKGVGEKAMEQLVEERRTNGPFKSLDDFAERVDPRLLNRRQIESLAGGG
                     AFDTIAANRAAVFAAAETILAHAASAADARTSGQGGLFGGESNVVPIRMPAAESWSLA
                     QRMTAEKESFGFYFSAHPVDRYRHLAIAHGARSFAEIGSLPAPAEGGRSAAVMTGLIE
                     EARWRTSARGRRYLMATVSDASGQFVATVFDDAIAAQVEDAAKSGGCALLNVELDRRP
                     GEEAPRVTIRSLQSFESLSKRARLQLEVELDGPEALARLAAAVADHRGGTGQLRLRAS
                     LDAGEAEIVLGRDFLLDAELAARIERLDGVTSVRLAVAEPPRLALVS"
     gene            complement(7688..8710)
                     /locus_tag="VF589_02430"
     CDS             complement(7688..8710)
                     /locus_tag="VF589_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256465.1"
                     /translation="MDFRKIDNRHKALLGIAALLLVLAAALGLSLTQQDDPPGAETKV
                     DLAAKQDEQTSDACASSATYDRLKEIAFEEAIRVRNADPANLDTLATHSVVRMENPVV
                     KSSDKDLNVTVCSGRFVLELPPGAERGFGGERRLSADIEYSAQSAADGSGLVYQIEGA
                     EPIIYKLAAFDLKGQSFQAPAPAAGADLAQADQPDASAAPPASQPERPQLAEAAPPPM
                     VPKAEPRSTPAPAPRSEASPEAEPSERPATASRGSARPSFNCRQAGSRSEQMVCGNRR
                     LAGLDRTMSSQFYSALARADPETRRELRRSRDRFLAYRERCRSEACVADAYQGRMAEI
                     SDILGE"
     gene            complement(8761..9432)
                     /locus_tag="VF589_02435"
     CDS             complement(8761..9432)
                     /locus_tag="VF589_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8256466.1"
                     /translation="MSDVLRVTGLKRSFKQGEAVIHVLRGIDLTVGDGEIVALVGPSG
                     SGKSTLLQAVGLLEGGFEGSIQINGEEAARLDNDGRTRLRRDALGFVYQFHHLLPDFN
                     AAENVILPQLIHGAETEPARQRAELLLGSLGLEHRLTHPPSKLSGGEQQRVAVARALA
                     NRPSLVLADEPTGNLDEATADVVLAEFLRLVRGEGSAALVATHNERLAAKMDRVVRLH
                     EGVLE"
     gene            complement(9425..10675)
                     /locus_tag="VF589_02440"
     CDS             complement(9425..10675)
                     /locus_tag="VF589_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX8256467.1"
                     /translation="MILSRYERMIAKRYLLPGKGEGFIFLVAGISLFAVTLGVAALVI
                     VMSVMNGFRAELFDKVVGLNGHAVVQGYGGRLDNWQQIVKQARATPEVTDAVPLIEQP
                     LMASFEGRVEGVLVRGMRREDILRNPTLQGKVLAGSLGALQPGSDNVAIGVRLAEALG
                     ANVGGQISLISPEGRTTPFGTVPRIVSYTVAAIFEVGVYDYDKAFVIMPLEDAQTFLM
                     LGDSVGMVEIETVDADRVGEILAPLAAAVTGDGHVADWRSMNQALFEALSVERVAMFV
                     ILSLIILVAVFNILSSLIMLVRAKTRDIAILRTMGASRRSLLKIFMTVGVTIGTLGTV
                     AGLVLAAIALFFRQSVVNFVQLLTGQNLWDPSIRFLTELPSKTDPFEATAVTVMALGF
                     SFLATLYPAYKAASTDPVQVLRYE"
     gene            complement(10794..>12000)
                     /locus_tag="VF589_02445"
     CDS             complement(10794..>12000)
                     /locus_tag="VF589_02445"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX8256468.1"
                     /translation="QTAAGIYAWLPLGLRVLDKIAAIVRQEQDRAGAVEMLMPTVQSA
                     DLWRQSGRYDAYGPEMLRINDRHDREMLYGPTNEEMITAIFRDAVKSYRDLPRTLYHI
                     QWKFRDEVRPRFGVMRGREFLMKDAYSFDLDEEGLKASYEAMFVAYLRTFARLGLQAV
                     PVRAPTGPIGGDLSHEFHILAETGESALFYDAAIEEVTREELLTADASTIARLTGLYA
                     MEEEEHAKVADCPVPPERLRTRRGIEVGHIFAFGTKYSAAMGFSVQTGDGGQVHPQMG
                     SYGIGVSRLVGAIIEASHDDAGIVWPEGVAPFRVGLINMRADDGSVSSAADEIYGRLQ
                     QAGVDTLYDDRDERGGAKFATMDLIGLPWQLIVGPKGLANGVVELKRRATGEREELSI
                     ESALSRLTR"
BASE COUNT         1885 a         3887 c         4304 g         1924 t
ORIGIN      
        1 cgatcccgag ctgatcgccg ccggcagcta ttggacggcg cgcgccgaca tgatgtgcgg
       61 ccggcccgag cgcgtccagg cgcggctgcg gaccgccgcg cggctcaagg aaacctttta
      121 cggcctgctt gccgccaagg cgctgggcat caagaccggc aatttcgccg ggctccacga
      181 tttccgcgac gccgaatggc ggagcatcgc cggcaagccc aacgtcaagg cggcaatcgc
      241 gctgaccgag atcggcgaag cggggctggc ggaggagcat atcaagcacc aggcgcggat
      301 cggcagccgc cgcgaccatg acgcgctgct ccacctcgcc tccgacctca acctgcccgg
      361 cacccaatat tggctcgccc acaacgcgcc gccgggaagc tatgtgaacc tcgccgcgcg
      421 ctatcccgcg cccgactggc ggccgagccg cggctggcgg gtcgacaagt cgctggcctt
      481 cgcccacgcg ctccaggaat cgagcttccg caccagcgtc gtcagcccgg cgggcgcgcg
      541 cgggctgatg caggtccggc ccggaacggc cggcgacatc gccaagaagc gcggggaaag
      601 cttcgacccg agccacctca acgaccccgc cgcgaacctc gaatatggcc aatcctatct
      661 cgaatatctg cgcagccacc acggcaccgg cgggctgctg cctaaggtga tcgcggccta
      721 taatgccggc ccgaacccga ttgcggagtg gaattatcgc cgcatggatc agggcgatcc
      781 gctgctctac atcgaatcga ttccttattg ggaaacgcgc ggctatgtgc cgatcgtgct
      841 tcgcaattac tggatctacg agcagcagga aggacgcgac tcggccagcc gccatgctct
      901 ggccgaaggc aaatggccgc gcttcccggg ccttcccggg gctaccgcgg tgcggatcgg
      961 ccccgccggg ggagcgagcg gcgggaccaa ctaatccgtt cttgatttgt tccaaaggta
     1021 ggtctatggc ggcgggatgc atcgtcccga cgcccgtgcc ggccgcggcg cccccgagaa
     1081 ccggaccccg acccgattca acctcaagga gcgcgaggcg gacggcgact ggctcgacgc
     1141 gcgcgaggcg ctgggcgaag gcgcgccgcc gctccggacg agcgtgacga tcgagacgcc
     1201 gaagacgatc atcacccgca acctctcgcc cgatgtcggc ttcgaccgct cgatcaacgc
     1261 ctatcgcggc tgcgagcatg gctgcatcta ttgcttcgcg cggcccaccc acgccttcca
     1321 cgatctctcg ccggggctgg atttcgaatc gaagctgttc gccaagcccg acgccgccaa
     1381 gctgctgcgc gaggaattgg gcaagcgcgg ctatgtcgtg cggccgatcg cgatggggac
     1441 caacaccgat ccctatcagc cgatcgaggc cgaataccgg atcacgcgcg cctgcatcga
     1501 ggtgctgctc gagactcggc atccgctgac catcaccacc aagtcggacc gcgtgctgcg
     1561 cgacctcgac ctgttggcac cgatggccga gctcggcctg gtcagcgttg cgctctcgat
     1621 cacttcgctg acgccggaaa tctcgcgaac gctggagccg cgggcgccgg ccgcgcgcaa
     1681 gcggctggcg gcggtcaagc ggctgggtga agccggcgtg ccggcctggg tggcgatcgc
     1741 gccggtggtg ccgggcatca ccgaccatga gctggaggcg atcgtcgagg ccgcggccga
     1801 ggcgggcgcg aagggcgctt tctatttgcc ggtgcggctg ccgcatgagg tggcgccgct
     1861 gttccgcgcc tggctcgacg aacattatcc cgagcgcgcc gccaaggtga tgcacatcat
     1921 ccaggcgatg cgcggcggca aggataacga ccccaattgg ttcacccgca tgcagggctt
     1981 cgggccgtgg gcggagctgc tgcgcacgcg gttcgagaaa gcggcgagga agcacggact
     2041 aagcggcgtc aagaacagcc tgcgcaccga cctgttcagg ccgccgcagg gcccgcagct
     2101 gcggctgttc tagatcctcc ctggcctctt gccggggaga gggaccgccg ccgcaggcgg
     2161 tggtggaggg gcggcgcgaa gcgcccctcg actgagcgat ctacctcaac cgccttcgga
     2221 aaactgagga ggatttaggc gcttcgcgcc gcccctccac cgcctgcggc ggtcccctcc
     2281 cctgcaaatg caggggagga ttctaggcgg cggtggggag tttgtggtgc ttgtactgct
     2341 ccctgagcgc ggtcttgagg agcttgccgg tggcggtgtg ggggagggtt tcgacgaaga
     2401 ggatctcgtc ggggagccac catcgggcga cgtgctgggt gaggtggcgg gtgatttcgg
     2461 cggggctgac gtcggagcct ggcttgcgca ccactagcag cagagggcgt tcgtcccatt
     2521 tggggtggtg gacgcccacc gcggcggctt cggcgacgcc ctcgcagccc atggcggcgt
     2581 tttcgagctc gatcgagctg atccactccc cgcccgactt gatcacgtcc tttgagcggt
     2641 cggtgatctg catcaccccg tccgggtgga ggatggcgac gtcgccggtg tcgaaccaat
     2701 tgtcctcgtc gacggcaggg ccgctcgcgt ccttgaagta gcgctggacc acccacggcc
     2761 cgcgcgcctg gaggcggccg gacgtcttgc cgtcgcgggg gaggacggtg ccgtcgtcgg
     2821 cgacggtgcg cagctcgact ccgaacggca ccctcccctg cttcaccata tagtcgatcc
     2881 gctcgtcctg gccgagctcg tcccaatggg cgggggcggc accggtggtg ccgatcggcg
     2941 aggtttcggt catgccccag gcgtggccga cgcggatgcc gcgctcgacg aagtagcgga
     3001 tcatcgaacg cggggcggcc gagccgccga tggtgacgac ctcgagcttg ccaaggtcgg
     3061 cgccggtcgc ctcgacatga ttgatgacgg cgagccagac ggtggggacg ccggcggtgt
     3121 gggtgacgcc ttcctcgcgc atcaggtcgc acatcacgct cggcgtgtaa tcggccgaga
     3181 agacgagctt taccccggcg agcggcgccg cgaagggcaa cccccaggcg gcggcgtgga
     3241 acatcggcac gatcggcagc gccacggccc gcgccgacag gtcgaatatg tcggggctga
     3301 cctcggccat ggcgtggagg acggtcgagc gatgctcgta gagcacgccc ttcgggttgc
     3361 cggtggtgcc gctggtatag cagagcatcg ccggctcgcg ctcgtcgccc tcgacccagc
     3421 gataatcgtc gctttcggag gcgatcagat cttcgaagcc gctctccccc tcggccgggt
     3481 cgaagcagac ataatgttcg atcgtgccga gctgcggctt cagccgctcg acgatcggcg
     3541 cgaaggcgcg gtcgtagaac agcacccggt cctcggcatg gttggcgatg tagacgagct
     3601 ggtcgtcgaa caggcgcgga ttgatggtgt ggaggatggc gcccatgccg atcgcgccat
     3661 accaggcgac gaggtggcgg ccgtgattca tggcgagggt ggcgacacgg tcgccgcgcc
     3721 tgacgccgag ccgctccagc gcgttggcaa ggcggcgggc gtcgcgggcg atgccggcgt
     3781 agttcgtgcg tatccgctcg ccggaagcgt agagcgagac gatctcgcgc ggtccgtgct
     3841 cgcgcccggc atggtcgagc agccgcatga tgcgcagcgg ccaatcctgc atcccaccga
     3901 tcatccgttc ctcccacatt atcggggcgg aggctggggt gtgtttgcgt tcgtgtcgag
     3961 tcggaaagga gaagggagaa gggagaaggg agaagggaga tcccagcttt cgctgggatg
     4021 acgggccagc gccttcgggt agccgtcagg agaccagggc gaggcggggc ggttcggcga
     4081 cggcgaggcg gaccgaggtg acgccgtcga gccgctcgat gcgggcggcg agctcggcat
     4141 cgagcaggaa gtcgcggccg aggacgatct cggcctcgcc ggcatcgagg ctagcgcgca
     4201 ggcggagctg gccggtgccg ccgcgatgat cggcgacggc ggcggcgagg cgggcgagcg
     4261 cctccggacc atcgagctcg acctcgagct ggagccgggc gcgcttggac aggctttcga
     4321 acgattggag gctgcggatc gtcacgcgcg gcgcttcctc gcccgggcgg cggtcgagct
     4381 cgacgttgag cagggcgcag ccgccgctct tggccgcatc ctcgacctgg gcggcgatgg
     4441 cgtcgtcgaa cacggtggcg acgaattggc cggaggcgtc cgagacggtc gccatcaaat
     4501 agcgccggcc ccgcgccgaa gtgcgccagc gggcctcctc gatcaggccg gtcataaccg
     4561 ccgccgaacg gccgccttcg gcgggagcgg gcagcgagcc gatctcggcg aagctccgcg
     4621 cgccatgcgc gatggcgagg tggcgatagc gatcgaccgg atgggccgag aaatagaagc
     4681 cgaagctttc cttttccgcc gtcatgcgct gggcgagcga ccagctttcg gccgcgggca
     4741 tgcggatcgg gacgacgttc gattcgccgc cgaacaggcc gccctgcccg ctggtgcgcg
     4801 catcggcggc gctggcggcg tgggcgagga tggtctcggc ggcggcgaac acggcggcgc
     4861 ggttggccgc gatcgtgtcg aacgcgccgc cgccggccaa gctttcgatc tggcgccggt
     4921 tcaggaggcg cggatcgacg cgctcggcga aatcgtcgag cgacttgaac gggccgttcg
     4981 tccgccgctc ctcgaccaat tgctccatcg ccttttcacc gacgcccttc agcgcgccga
     5041 gggcatagcg aacggccaat ctgtgctcct gcgaaggcag gagcccagtc tgggttcctg
     5101 ccttcgcagg aacactgggg agcaattcca cggagaattc ggcctcgctg gcgttgatgc
     5161 tcggcggcag gcaggcgact tcgagccggc gcatgtcttc gacgaagatc ccgagcttgt
     5221 cggtctgggc catgtcgaag cacatcgagg cggcgtagaa ttcgaccgga tgatgcgcct
     5281 tcagccacgc cgtctggtag gcgaccaaag cgtaggccgc ggcgtggctc ttgttgaagc
     5341 cgtagccggc gaacttgtcg atcaagtcga acagctcgtt ggccttgggg cggccgatct
     5401 ggttggcgcc gcagccgctg acgaaacgct cgcgctgggc gtccatctcg gccttgatct
     5461 tcttgcccat cgcgcggcgg agcaggtcgg cctcgccgag gctgtagccg gcgagcacct
     5521 tggcggcctc catcacctgc tcctgataga cgaagatgcc gtaagtctcc ttcagcacgt
     5581 tctcgagcat gggatgggga tattcgatcg gctcgcggcc gttcttgcgg gcgccgaaca
     5641 tcgggatatt gtccatcggg cccggccggt agagcgacac cagcgcgatg atgtcctcga
     5701 agccggtcgg tttcacggcg gcgagggtgc ggcgcatgcc ttcggattcg agctggaaca
     5761 cgccgaccgt ttcgccgcgc tggagcagct tgaagacttc ggcatcgtcc caggggatgc
     5821 ggtcgagatc gacggtgatg ccgcgcttgg cgagcatctg gaccgccttc tggagcacgg
     5881 acagcgtctt gaggccgaga aagtcgaact tcacgaggcc cgccgcttcg acatatttca
     5941 tgtcgaactg ggtgaccggc atatccgagc gcggatcgcg gtacagcggg acgagcttgt
     6001 cgagcggccg atcgccgatc acaacgccgg cggcgtgggt cgagctgtgc cggggcaggc
     6061 cttcgagctt gcgcgccaga tcgaacaggc ggcggacttg ggcgtcgttc ttatattcgg
     6121 tggcgagctc ggagacgccg ttcagcgccc gctcgaggtc ccaaggctcg gtcgggtggt
     6181 tggggatcag cttggccaac cggtccacct ggccataggg catttgcagg acgcgcccgg
     6241 tatccttgag cacggcgcgc gccttcagct taccgaaggt gatgatctgc gcgaccttgt
     6301 cggcgccgta gcgctgctgg acgtagcgaa tgacgtcgcc gcggcgggtt tcgcaaaagt
     6361 cgatgtcgaa atccggcatc gacacgcgct cgggattgag gaagcgctcg aacagcaggc
     6421 cgagccggat cggatcgaga tcggtaatgg tgagcgacca ggcgaccacc gagccggcgc
     6481 cggagccgcg gcccggcccg accgggatgt cgtgctcctt ggcccatttg atgaagtcgg
     6541 cgacgatcag gaaataagcg gcgaagccca tgccgacgat gatctcgact tcatagtcga
     6601 gccggtcgaa ataggctttg tagcgggttt cgagggctcc cccggtaagg gcggcgtccg
     6661 ggtcgggcat ccctgggccc cggccttcgc cggggagctg gtagtccgcg aggcgcgcct
     6721 ccagcccggc gcgggcgtcg cggcggagct gctccgcctc cgcttcgagg tcgccggcga
     6781 tgcgggggag gatcgggctg cgcttggggg ctccgaacgc gcagcggcgg gcgacgaccg
     6841 acgtgttggc gatcgcctcc ggcagatcct cgaacagctt gcgcatgtcg gcggcgggct
     6901 tgatccaggc ttcgggcgag gatttcgcgc ggtcgtcgcg gtcgatctgg ctcgactggg
     6961 cgatgcacag catcgcgtca tgggcccggt ggaaggctgg gtcggtgaag caggcggggt
     7021 tggtggcgac cagaggcaga tcgcgctcgt aagcgagatc gatcaaagcc ggctcggcgc
     7081 gctgctctac ctcgtccccg cgccggcaaa gctcgacgta gagccggccg gggaacagcg
     7141 cctgaagccg gtcgagcacg gcgagcgcgg cggcattctg ctcctccgcc agcagccgcg
     7201 cgagcgcgcc ttcgccgccg ccggtgagcg cgatcaggcc gtcggtgcga ccttcgagcg
     7261 cgtcgaagcc gacatgcgcc tcttcctcca gcggccggcc gagatgggcc gtggaaacca
     7321 gcgcgcacag attctggtag ccggcctcgt cctgcgcgta gagcgcgagc cagtcgatct
     7381 gcgcgcccct ggcgccggcc gcgccgggcc gggcgaggcc gagcatggtg ccgatgatcg
     7441 gctggacccc ctcggccttg caggcgtcgg tgaagggcat cgccgcataa agcccgttgc
     7501 ggtcggtcaa ggcgacggcc gggaattcga gcttcttcgc ctgcttggcg atcgccttgg
     7561 gctcgatcgc cccttccagc atggtgtagc aggagaagat gcgcaggggg acgtaaggcg
     7621 cggccatcgg acgtttatga gcggcgcgcc ggccaaaggg aactggggcg ggtcattttt
     7681 ccacagctta ttcgccgagg atgtcgctta tctcggccat ccggccctga taggcgtcgg
     7741 cgacgcaggc ttcgctgcgg cagcgctcgc gataggcgag gaaacggtcg cgcgagcggc
     7801 gcagctcgcg ccgggtctcc gggtcggcgc gcgccaaggc cgaatagaat tgcgacgaca
     7861 tggtccggtc gagcccggcg aggcggcggt tgccgcacac catctgctcg ctgcgggagc
     7921 cggcctgtcg gcagttgaag ctcggccggg cgctgccgcg gctggcggtg gcgggccgct
     7981 cgctcggctc ggcttcgggc gacgcttccg agcgcggcgc gggcgcgggc gtggagcgcg
     8041 gctccgcctt gggtaccatc gggggcggcg cggcttcggc cagctgcggc cgctccggct
     8101 gcgaggcggg gggcgcggct gaagcgtcgg gctggtccgc ttgggcgagg tcggcgcccg
     8161 cggccggggc cggcgcctgg aagctctggc ctttgaggtc gaaggcggcg agcttgtaga
     8221 tgatcggctc ggcgccttcg atctggtaga cgaggccgct gccgtcggcg gccgactgcg
     8281 ccgaatattc gatgtcggcg ctgagacggc gctcgccgcc gaagccgcgc tcggcccccg
     8341 gcggaagctc gagcacgaac cggcccgagc agacggtgac gttgaggtcc ttgtcgctgc
     8401 tcttcacgac cgggttttcc atgcggacga cggaatgggt ggcgagcgtg tcgagattgg
     8461 cgggatcggc gttgcggacc cggatcgcct cttcgaacgc gatctccttc agccggtcgt
     8521 aggttgcgct ggaggcgcag gcgtcgctgg tctgctcgtc ctgcttggcg gcaagatcga
     8581 ccttggtctc cgcgcccggc ggatcgtcct gctgagtaag gctgagcccg agggccgccg
     8641 ccagcacgag cagcaatgcg gcaatgccca gcaacgcctt gtgcctgttg tcgattttac
     8701 gaaaatccat cgctctacgc cctcacgaaa ccggtgcccg gcggtggagc gacgacgggg
     8761 tcactccagc acgccctcgt gcaaacgcac aactcggtcc atctttgcag ccagccgttc
     8821 attatgagtc gcgaccaggg cggcgctgcc ttcgccgcgg acgaggcgga ggaattccgc
     8881 cagcacgacg tcggccgttg cttcgtcgag attgccggtc ggctcatccg ccagcaccag
     8941 cgacggccgg ttggcgagcg cgcgcgcgac cgccacgcgc tgctgctcgc cgccggagag
     9001 cttgctgggc ggatgggtga gccggtgctc cagccccagg gagccgagca gcaattcggc
     9061 ccgctggcgc gccggctcgg tctcggcgcc gtggatgagc tgcggcagga tcacgttctc
     9121 ggcggcgtta aaatccggca gcaggtggtg gaattggtag acgaagccga gcgcgtcgcg
     9181 gcgcaggcgc gtgcggccgt cattgtcgag ccgggcggct tcctcgccgt tgatctggat
     9241 cgaaccttcg aagccgcctt cgagcaatcc caccgcctgg agcagggtcg acttgcccga
     9301 gcctgagggc ccgaccagcg cgacgatctc gccgtcgccg accgtgagat caatgccgcg
     9361 caggacgtgg atcacggcct cgccctgctt gaagctgcgc ttcagcccgg tgacgcgcag
     9421 cacgtcactc atagcgcagc acctggaccg gatcggtgct cgccgccttg taggccgggt
     9481 agagcgtggc gaggaagctg aagccgagcg ccatgacggt gaccgcggtc gcttcgaacg
     9541 gatcggtctt ggacggcagt tcggtgagga agcggatcga gggatcccac agattctgcc
     9601 cggtgaggag ctggacgaaa ttcaccaccg actggcggaa gaacagcgcg atcgccgcca
     9661 ggaccaggcc ggcaacggtg ccgagcgtgc cgatcgttac gccaaccgtc atgaagatct
     9721 tgagcaggct gcggcgggac gcgcccatcg tgcgcaggat ggcgatgtcg cgggtcttgg
     9781 cccgcaccag catgatgagc gaggacagga tgttgaacac ggcgaccagg atgatcagcg
     9841 acaggatgac gaacatcgcg acgcgctcga ccgaaagcgc ctcgaacagc gcctggttca
     9901 tcgaccgcca gtcggcgacg tgcccatctc ccgtcacggc ggcggcgagc ggcgccagga
     9961 tctcgccgac gcggtcggca tcgacggtct cgatctcgac catgccaacc gagtcgccga
    10021 gcatcaggaa ggtctgcgcg tcctcgagcg gcatgatcac gaacgccttg tcgtaatcgt
    10081 agacgccgac ctcgaagatg gcggcgacgg tataggagac gatccgcggc accgtgccga
    10141 acggcgtggt gcggccttcc gggctgatca ggctgatctg gccgccgaca ttggcgccga
    10201 gcgcctcggc caggcggacg ccgatcgcga cattgtcgga cccgggctgg agcgcgccga
    10261 gcgagccggc gagcaccttg ccctggaggg tggggttgcg caggatatcc tcgcgccgca
    10321 tgccgcggac gagcacgccc tcgacgcggc cctcgaagct cgccatcagc ggctgctcga
    10381 tcagcggcac cgcgtcggtg acctcgggcg tggcacgggc ctgcttcacg atctgctgcc
    10441 aattgtcgag ccggccgcca tagccctgga cgacggcatg gccgttgagg ccgaccacct
    10501 tgtcgaacag ctcggcgcga aagccgttca tgacgctcat gacgatgacg agggcggcga
    10561 cgcccagcgt gaccgcgaac aggctgatgc cggcgaccag gaagatgaag ccctcgccct
    10621 tgccgggcaa cagatagcgc ttggcgatca tgcgctcgta gcgggagagg atcatgccct
    10681 caccctccca ttgctgacgc aatgggcccc tccctctccc ttgaagggag aggggtgttt
    10741 cgtgggtgac agaagaagtg agattccagc tttcgctgga atgacgaggg tgctcatctc
    10801 gtgagcctcg acagcgcgct ttcgatcgag agctcctcgc gctcgccggt ggcgcggcgc
    10861 ttgagttcga cgacgccatt cgcgaggccc ttcgggccga cgatgagctg ccagggcagg
    10921 ccgatcaggt ccatggtcgc gaacttggcg ccgccgcgtt cgtcgcggtc gtcgtagagc
    10981 gtgtcgacac cggcctgctg cagccgcccg taaatctcgt cggcggcgct ggagacgctg
    11041 ccgtcgtcgg cgcgcatgtt gatgaggccg acccggaacg gcgcgacgcc ttcgggccag
    11101 acgatgccgg catcgtcatg gctcgcctcg atgatcgcgc cgaccaagcg ggagacgccg
    11161 atgccgtagc tgcccatttg cggatggacc tggccgccgt cgccggtctg gaccgagaaa
    11221 cccatcgccg cgctgtattt ggtgccgaag gcgaagatgt ggccgacctc gatgccgcgg
    11281 cgggtgcgca gccgttccgg cggcaccggg cagtcggcga ccttcgcatg ctcttcctcc
    11341 tccatcgcat aaaggccggt gaggcgggcg atggtcgagg cgtcggcggt gagcaattcc
    11401 tcgcgggtca cctcctcgat ggcggcgtcg tagaagagcg cgctctcgcc ggtttcggcg
    11461 aggatgtgga attcgtggct gaggtcgccg ccgatcggcc cggtcggcgc gcggaccggt
    11521 accgcctgga ggccgaggcg ggcgaaggtg cgcaggtagg cgacgaacat cgcctcgtag
    11581 ctcgccttca ggccttcttc gtcgagatcg aagctgtagg cgtccttcat caggaactcg
    11641 cgcccgcgca tcacgccgaa gcgggggcgg acctcgtccc ggaacttcca ctggatgtgg
    11701 tagagcgtcc gcggcaagtc gcgatagctc tttaccgcat cgcggaaaat cgccgtgatc
    11761 atctcttcgt tcgtcggacc gtagagcatt tcccggtcgt gccggtcgtt gatccgcagc
    11821 atttcggggc cgtaggcgtc gtagcggccg gactggcgcc acaggtcggc cgactggacg
    11881 gtgggcatca gcatctcgac cgcgccggcc cggtcctgct cctggcggac gatggcggcg
    11941 atcttgtcga ggacgcgcag cccgagcggc agccaagcgt agatgccggc ggcggtctgg
//
LOCUS       DASVRK010000071        11151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_572126, whole genome shotgun sequence.
ACCESSION   DASVRK010000071 DASVRK010000000
VERSION     DASVRK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572126"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VF589_02450"
     CDS             complement(<1..302)
                     /locus_tag="VF589_02450"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX8256469.1"
                     /translation="MALLLARIQFAFTISFHFIFPAFSIGLASYLAVLEGLWLRTGKA
                     VYLNLFRYWLKIFAIAFAMGVVSGIVMSYQFGTNWSVFSDRAGPIIGPLMAYEVLTA"
     gene            503..2095
                     /locus_tag="VF589_02455"
     CDS             503..2095
                     /locus_tag="VF589_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8256470.1"
                     /translation="MRIKGWALGAAMLALTGAAAAAPVGGLKGRAVAGVEQRAKLAQE
                     INDSIFSFAELGFQEVETSRYLTQLLEKQGFTVERGVSGLPTGWVARWTNGAGGPVIA
                     LGSDIDGIPKASQKPGVPYRAPMVEGAPGHGEGHNSGQAVNVVAALAVKELMERENIP
                     GTLMLWPGVAEELVAGKAYMVRDGLFRDVDAVLFTHVGDNLETAWGRPMGTGLVSVEY
                     RFEGESAHSAGAPWRGKSALDAVELMNMGWNMRREHLRPEQRSHYVITDGGDQPNVVP
                     STASVWYYFREQDFKGIARNFETGNKIADGAAMMTGTKVSRRILGTAAPRHFNRPIAE
                     AAYANIRAVGLPRWTAEEQAFAKLVQKNVAADKQEGLAVKLAELKPPLDEPKSGGSDD
                     IGDVSWVVPTITIRYPANIPELPGHNWSNAISMATPIAHKGVVAGAKVVAMTTLDLLT
                     KPQLRKSARDYFTGVQTKDEKYAPVLGAGDKPAIEINSDVMARYKPELRKYYYDPARY
                     PTYLEQLGIQWPMLGSAPAAGQ"
     gene            2092..3342
                     /locus_tag="VF589_02460"
     CDS             2092..3342
                     /locus_tag="VF589_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8256471.1"
                     /translation="MMRRLIACALALLAVPAGAQEQAILLRPARVYDGVDPRPHVDWA
                     VLVRGDRIAAAGPNLAAPAGARVIDLPGTTLMPGMIEGHSHLFLHPYDETSWDDQVLK
                     EPESLRTARAVNHARATLMAGFTTVRDLGTEGAGYADVGLKQAIDEGIVPGPRMLIAT
                     RALVATGSYGPKSADPEAPLRGAEEADGPGLVTAVRRQIGGGADVVKFYGDYGWRPGE
                     DSRPTYTLGELSAAVEAAHAAGREVAVHASTPAAMRSAILAGADTIEHGNGGTTEIFR
                     LMRERGVALCPTLAASDAIARYRGWNGAAPEPARVREKRASFAAALAAKAPICMGGDV
                     GVYAHGDNAREMELMVAYGMPAPDVLAAATSGNARLFGLADRGAVRPGLLADLVAVEG
                     DPTRDISAVRAVRLVMKGGKVVRE"
     gene            complement(3437..4393)
                     /locus_tag="VF589_02465"
     CDS             complement(3437..4393)
                     /locus_tag="VF589_02465"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEX8256472.1"
                     /translation="MNADRPVGSYDFDIEPILDDVFSYSETYRGHGTLASYIPVLAKI
                     DPMKLGIAVVLGDGSVHCRGDADELFSIQSISKVFTLSLALQHAGPALWNRVGREPSG
                     SPFNSIVQLEAEKGKPRNPLINAGAFVVCDHLIHEAGADGALAELLMHLRERAGSETI
                     AADETVAMSESQAGYLNRSLANFVKAFDNLDNPVEEVLSLYFRQCSIAMSCRQLARAA
                     LHLAFDGIDPVSGDQIMIASRSRRINALMLTCGHYDNSGDFAFRIGLPGKSGVGGGIL
                     AIVPGIGAICVWSPGLNAAGTSLAGAVALEKLVERTGWSIFV"
     gene            4533..6311
                     /locus_tag="VF589_02470"
     CDS             4533..6311
                     /locus_tag="VF589_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HEX8256473.1"
                     /translation="MCGIAGEVRFRSGIADADAVAKMMRSQARRGPDACGQLMRGRVG
                     LGHQRLKIIDLSPRSEQPMVDPELGLSLVFNGCIYNYQPLRDELKALGYHFFSGGDTE
                     VIIKAWHAWGRACVERFKGMFAFAIHERETGRVALARDRFGIKPLYYSQTPDRLRFAS
                     TLPGIVAAGDVDTSIDPQALQFYMSWHAVVPPPWTILKGVRKLPPATVRIYEEDGSFA
                     DSVYWDPPFGQTAEDAGRSAGEWEDMLLASLRTAVERRMVSDVPVGVLLSGGVDSSLI
                     VGLLAEMGQHGLATFSIGFEEAHGEKGDEFQYSDLIANHYGTDHHQLFIPSADLLNEL
                     PGAIHAMAEPMVSYDNVGFYLLSREVAKHIKVVQSGQGADEVFGGYHWYPPMAGANDP
                     VATYAKAFFDRDGAALGAQLGPAWRNGGTLPLDYVREHFARPGASDPVDKALRIDSTV
                     MLVDDPVKRVDNMTMAWGLEARVPFLDHDLAELAARIPSGLKLAGGGKGILKDVARKV
                     IPAEVIDRPKGYFPVPALKYISGPYLDLVRDALTGQAARERGLFAGDYLDALFADPTG
                     HITPLRGSELWQVGLLELWLQQHKVG"
     gene            6311..8029
                     /gene="ngg"
                     /locus_tag="VF589_02475"
     CDS             6311..8029
                     /gene="ngg"
                     /locus_tag="VF589_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HEX8256474.1"
                     /translation="MRTRFRPIRPHPPGGKPLKPNALADCGWGRVLFAHTFEDVAELV
                     AEIRAEGPAERDIALYVTDPHVLIASAPAELFLDPSHTYRLNLATYRPWQRKPPGFMV
                     RRMVADTDADAVNRLYALHRMVQVRPDFFDQESQGRLITYFVAEDEETGAVIGTVTGI
                     DHAAAFDDPEHGSSLWTLAVDPQTAHAGVGEALIRWLAEHYIARGAGYMDLSVLHDNH
                     GAIALYEKLGFHRLPVFAVKRRNVINERLFAGHDPAKDMNPYARIIINEARRRNIGVD
                     VIDAAGGLFQLHSGGRSVRCRESLSELTSAVALSICDDKAMTRRIVEAAGVTVPEEVE
                     ASDGQAVRDMLAKHGSLVVKPARGEQGRGVAVDLDTIESVDRAIEAARAHSEQVLVES
                     FHKGEDLRLIVIGFRLVAAAVRRPAEVVGNGRDSVRQLIERQSRRRAAATGGESRIPL
                     DAETERCVAKAGFAMDEVPPEGATFPVRKAANLHTGGTIHDVTASLDPQLAEAAIRVA
                     RAIDIPVVGVDFIVTSPDQPDYVFIEANERPGLANHEPQPTAERFIDLLFPLSMPAAR
                     HHHEGG"
     gene            8150..9301
                     /locus_tag="VF589_02480"
     CDS             8150..9301
                     /locus_tag="VF589_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HEX8256475.1"
                     /translation="MTGPQIDTDYLKTQLDRLLSIPSPTGYTDTVVRHVSQELERLGV
                     NYSVTRRGAILALYQGAEVEGARAIVSHLDTLGATVKMIKDNGRLELVPIGTWSARSA
                     ESSRCTIFTEEGSYRGTILPLKASGHTFNEEVDTQPVGWRHVEVRIDADCTDRKSAEA
                     LGIHIGDFVAIDTGTEFLDNGYIVSRHLDDKAGCAVALAALKALVDGNVATPVDIHFI
                     FTITEEVGSGASAVLLDNVVAMVSIDNGTTAPGQNSSEFGVTIGMADQVGPFDYHLTR
                     KLIRLCQENEIRHQRDVFRYYRSDSASALEGGADVRTALITFGIDASHGYERIHLHSL
                     RSLAELVVLYAGSPVEIARDVEELAGIAGFTEQPTEPARQAEDDPSMHE"
     gene            complement(9441..9950)
                     /locus_tag="VF589_02485"
     CDS             complement(9441..9950)
                     /locus_tag="VF589_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8256476.1"
                     /translation="MATQFAPASPPPSVAPLPFLFARPQVADRGWSFSSALAASVAAE
                     RADMTRRNTRGRIAYLLCELGYQLARRGEDRDTLPLTRGELATALGVSLCRVKRTLAL
                     LELSAVTASDGVQLRVLDWRRLCGVAHFQLGRIGLTADDDAEPLQIVEQDEAPANQLT
                     AAGDPACFV"
     gene            complement(10073..>11151)
                     /locus_tag="VF589_02490"
     CDS             complement(10073..>11151)
                     /locus_tag="VF589_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256477.1"
                     /translation="TTSNASLGKFLTLRAQAFRSNDYYPSELAWMDVAGTPIEVVIGP
                     YEVYTDSLYGTKTAFESFVTVQDPKESGALQKYKGLLRDMEANLPVEEKYKNFNRPFA
                     SPILVADQVHAGGDSVPGVQTVAFNLPNDERVREAKGAKKVILANVLGAKYDRILGPM
                     AQHVLVPDQARLVSRAYMTNETLFHELSHSLGPGTIAVGGRKTTVNAELKDVYSPSEE
                     AKADVMGMHNILYMMKRGELPAAEREQALATYFAGIFRAVRFGIDEAHGRGAAMQYSW
                     LKERGAFAWDPERRLFRVDYNALERGLGELTALLVRLQGNGDYAGTKAFFDRYAKLDD
                     QARAVLAATARIPTDIQPDYPDRI"
BASE COUNT         1866 a         3597 c         3939 g         1749 t
ORIGIN      
        1 gcggtcagca cctcataggc catcaaaggg ccgatgatcg gcccggcccg atcggaaaag
       61 accgaccagt tggttccgaa ctggtaggac atgacgatcc ccgacacgac gcccatcgcg
      121 aaggcgatcg cgaagatctt cagccagtat cggaacaggt tgaggtagac cgccttgccg
      181 gtgcgaagcc acaggccttc gagcacggcg agataggaag cgagcccgat cgaaaaagcc
      241 gggaagatga aatggaacga gatggtgaag gcgaactgga ttcgcgcgag caacaatgcc
      301 atttcgccgt cggtcacctg accccgcctc ccgttgccgc gcatgtagcg atttcgagcc
      361 cgcggttcac cccccgaagc ggttgatcaa aatcgtgcca caagtgcagc ttgtgcaatc
      421 acttgcgcgg cgggcggcgg cgttccaatg atcggcccaa cgaccggcgg agaccggacg
      481 cactgacacg gcaggggaaa agatgagaat caagggctgg gcgctcggcg ccgcgatgct
      541 ggctttgacg ggcgcggccg cggccgcccc ggtcggcggg ctcaagggac gggcggtggc
      601 gggggtcgag cagcgcgcca agctcgcgca ggagatcaac gattcgatct tcagcttcgc
      661 cgagctcggt ttccaggagg tggaaacttc gcgctatctg acccagctgc tcgaaaagca
      721 gggcttcacg gtcgagcgcg gcgtgtcggg gctgccgacc ggctgggtgg cgcgctggac
      781 caacggcgcc ggcgggccgg tgatcgcgct cggcagcgac atagacggca tccccaaggc
      841 ctcgcaaaag ccgggcgtcc cctatcgcgc gccgatggtc gaaggcgcgc ccgggcatgg
      901 cgagggccat aatagcggcc aggcggtgaa cgtggtcgcc gcgctcgcgg tcaaggagct
      961 gatggagcgc gagaatatcc ccggcacatt gatgctgtgg ccgggcgtcg ccgaggagct
     1021 ggtcgcgggc aaggcctata tggtccgcga cggactgttc cgcgatgtcg acgcggtgct
     1081 gttcactcat gtcggcgaca atctggagac cgcgtggggg cggccgatgg gcaccggcct
     1141 ggtctcggtc gaataccggt ttgagggcga gagcgcgcat tcggcggggg cgccgtggcg
     1201 gggcaaatcg gcgctcgacg cggtcgagct gatgaacatg ggctggaaca tgcgccgcga
     1261 gcatctgcgg cccgagcagc gctcgcatta cgtcatcacc gacggcggcg accagcccaa
     1321 cgtggtgccc tcgaccgctt cggtctggta ttatttccgc gagcaggatt tcaaagggat
     1381 cgcccgcaat ttcgagaccg gcaacaagat cgccgacggg gcggcgatga tgaccggcac
     1441 caaggtgagc cgccgcattc tcggcacggc ggcgccgcgc catttcaacc gcccgattgc
     1501 ggaggcggcc tatgccaata tccgcgcggt cgggctgccg cggtggacgg ccgaggagca
     1561 ggcgttcgcc aagctcgtgc agaagaatgt cgccgcggac aagcaggagg ggctggcggt
     1621 gaagctcgcc gagctgaagc cgccgctcga cgagcccaag agcggcggat cggacgatat
     1681 cggcgacgtg tcgtgggtcg tgccgaccat caccatccgc tacccggcca acatacccga
     1741 gctgcccggc cacaattggt cgaacgcgat ctcgatggcg acgccgatcg cgcataaggg
     1801 cgtggtcgcg ggcgccaagg tggtggcgat gacgacgctc gatttgctga ccaagccgca
     1861 gctgaggaag tcggcccggg attatttcac gggcgtccag accaaggacg agaaatatgc
     1921 gccggtgctg ggggcgggcg acaagccggc gatcgaaatc aacagcgacg tgatggcccg
     1981 gtacaagccc gagcttcgca aatattatta cgatccggcg cgttacccga cctatctgga
     2041 gcagctcggc atccaatggc cgatgctggg gagcgcgccg gcggcggggc aatgatgcgg
     2101 cggctgatcg cctgcgcgct ggcgctgctg gcggttccag cgggggcgca ggagcaggca
     2161 atattgctgc gcccggcgcg ggtgtatgac ggcgtcgacc cgcggccgca tgtcgactgg
     2221 gcggtgctgg tgcggggcga ccggatcgcg gcggcggggc cgaacctcgc cgcgccggcc
     2281 ggggcgcgcg tcatcgacct tccgggcacg accctgatgc cgggcatgat cgagggccat
     2341 tcgcacctct tcctccaccc ctatgacgag accagctggg acgaccaggt gctgaaggag
     2401 ccggagtcgc ttcggacggc gcgggcggtg aaccatgcgc gggcgacctt gatggccggc
     2461 ttcaccaccg tccgcgacct cggcaccgag ggcgcgggct atgccgatgt cgggctcaaa
     2521 caggcgatcg acgagggcat cgtgccgggg ccgcggatgc tgatcgcgac ccgcgctctg
     2581 gtcgcgacgg gcagctacgg acccaaaagc gcggaccctg aagcgccgct gcggggggcg
     2641 gaggaggcgg acgggccggg gctggtcacg gcggtgcggc gccagatcgg cggcggcgcg
     2701 gacgtggtca aattctacgg cgattatggc tggcggcccg gcgaggacag ccggccgacc
     2761 tacacgctcg gcgagctcag cgccgcggtc gaggccgccc atgccgcggg ccgcgaggtc
     2821 gcggtccatg cctcgacgcc tgcggccatg cggagcgcga tcctcgccgg cgccgacacg
     2881 atcgagcatg gcaatggcgg cacgacggaa atcttccggc tgatgcgcga gcgcggggtg
     2941 gcgctgtgcc cgacgctcgc cgcgagcgac gccatcgcgc gttatcgcgg ctggaacggg
     3001 gcggctccgg agccggcgcg ggtgcgggag aaacgggcga gcttcgccgc cgcgctggcg
     3061 gccaaggcgc cgatctgcat gggcggcgat gtcggcgtct acgcgcatgg cgacaatgcc
     3121 cgcgagatgg agctgatggt ggcttacggg atgccggcgc cggacgtgct ggccgccgcc
     3181 acctcgggca atgctcggct gttcggcctc gccgaccgcg gcgcggtgcg gccggggctg
     3241 ctcgccgacc tggtcgcggt ggagggggat ccgacgcggg atatttccgc cgtgcgggcg
     3301 gtgcggctgg tgatgaaggg cgggaaggtg gtgcgggagt aggtgggcaa cgtctagaag
     3361 agctcgcttc gctcgctgcc cctccaccgt gctgcgcacg gtccccctcc cccgggaagc
     3421 cgggggagga attgcactac acaaaaatcg accagccggt gcgttcgact agcttttcga
     3481 gggcgacggc gccggccagc gaggtgccgg cggcgttgag gccgggggac cagacgcaga
     3541 tcgcgccgat gccagggacg atcgcgagga tgccgccgcc gacgccgctc ttgccgggaa
     3601 ggccgatgcg gaaggcgaag tcgccgctat tgtcgtaatg gccgcaggtc agcatcagcg
     3661 cgttgatccg ccgcgagcgt gacgcgatca tgatctggtc gccgctgaca ggatcaatgc
     3721 cgtcgaaagc caagtgcagc gccgcccgcg ccagctgccg gcacgacatg gcgatcgagc
     3781 attgccggaa atagagcgac agcacctcct cgacaggatt gtcgagattg tcgaacgcct
     3841 tgacgaaatt ggcgaggctg cggttgaggt agccggcctg cgattccgac atggcgacgg
     3901 tctcgtcggc ggcgatggtc tcggagcccg cgcgttcgcg aagatgcatg agcagttcgg
     3961 cgagcgctcc gtccgcgcct gcctcgtgga tcagatgatc gcagacgacg aaggcgccgg
     4021 cgttgatcag cgggttcctc ggcttgccct tctccgcctc gagctggacg atcgagttga
     4081 acggcgatcc cgacggctcg cgcccgaccc ggttccaaag cgccgggccg gcatgctgga
     4141 gcgccagcga gagcgtgaag accttggaga tgctctggat cgagaacagc tcgtcggcgt
     4201 cgccgcggca atggacgctg ccgtcgccga gcacgacggc gatgccgagc ttcatcgggt
     4261 cgatcttggc cagcaccggt atgtagctgg cgagggtgcc gtggccgcga taggtttccg
     4321 aatagctgaa cacgtcgtcg aggatcggct cgatgtcgaa atcgtagctc ccgacgggcc
     4381 tgtctgcatt catgtggcgc ctttctcgaa ccgaaacgat ttgggggaag ccgggagcca
     4441 atcgagccgc ccacggttgt agggtcaatc cccttatccc aactttgatc cgaaatgaga
     4501 attgaacaag ctttgaaaag ggcggcgcgc gcatgtgcgg catagccggt gaagtaagat
     4561 tccgctccgg catcgccgat gccgacgcgg tcgcgaagat gatgcgcagc caggcccggc
     4621 gcgggccgga cgcgtgcggg cagctgatgc gggggcgcgt ggggctcggc caccagcgcc
     4681 tgaagatcat cgacctcagc ccgcgctccg agcagccgat ggtcgacccc gagctcgggc
     4741 tcagcctcgt cttcaacggc tgcatctaca attaccagcc gctgcgcgac gagctcaaag
     4801 cgctcggcta ccatttcttc tcgggcggcg acaccgaggt gatcatcaag gcctggcacg
     4861 cctggggccg cgcctgcgtc gagcgcttca agggcatgtt cgccttcgcg atccacgagc
     4921 gcgagaccgg gcgggtcgcg ctcgcccgcg accggttcgg catcaagcct ttatattatt
     4981 cgcagacacc cgaccggctg cgcttcgcct ccaccctccc tggcatcgtc gcggccggcg
     5041 acgtcgacac ctcgatcgat ccgcaggccc tgcaatttta catgagctgg catgcggtgg
     5101 tgccgccgcc ctggacgatc ctcaaaggcg tcaggaagct gccgccggcg acggtccgca
     5161 tctatgagga ggatggcagc ttcgccgaca gcgtctattg ggacccgccg ttcggccaga
     5221 ccgccgagga tgccggccgc agcgccggcg aatgggagga catgctgctg gcctcgctgc
     5281 gcaccgccgt cgagcggcgc atggtgtcgg acgtgccggt gggcgtgctg ctgtcgggcg
     5341 gggtcgattc cagcctgatc gtcggcctgc tcgcggagat gggccagcac gggctcgcca
     5401 ccttctcgat cggcttcgag gaggcgcatg gcgagaaagg cgacgaattc caatattcgg
     5461 acctgatcgc caaccattat ggcaccgacc accaccagct gttcatcccc tcggcggacc
     5521 tgctgaacga gctgcccggc gcgatccacg ccatggccga gccgatggtg agctacgaca
     5581 atgtcggatt ctacctgctc agccgcgaag tcgccaagca catcaaggtc gtgcaatcgg
     5641 gccagggcgc ggacgaggtg ttcggcggct atcattggta cccgcccatg gccggcgcca
     5701 acgacccggt cgcgacttat gccaaggcct ttttcgaccg cgacggggcg gcgcttggcg
     5761 cccagctcgg cccggcctgg cgcaacggcg gcacgctgcc gctcgactat gtacgggagc
     5821 atttcgcccg ccccggcgcg tccgacccgg tcgacaaggc gctccgcatc gattccacgg
     5881 tgatgctggt cgacgacccc gttaagcggg tcgacaatat gacaatggcc tgggggctgg
     5941 aggcgcgggt cccgttcctc gaccacgatc tggccgaact cgccgcgcgc atcccgtccg
     6001 ggctgaagct cgccggcggc ggcaagggca tattgaagga cgtggcgcgc aaggtgatcc
     6061 ctgccgaggt gatcgaccgg cccaagggct atttcccggt gcccgcgctc aaatatattt
     6121 ccgggcccta tctcgacctg gtgcgcgacg cgctcaccgg ccaggcggcg cgcgagcgcg
     6181 gcctgttcgc gggcgactat ctcgacgcct tgttcgccga tccgactggc cacatcacgc
     6241 cgctgcgcgg gtccgagctg tggcaggtgg ggctgctcga attgtggctc caacagcaca
     6301 aggtcggatg atgcgcaccc gcttcaggcc gatccggccg catccgccgg ggggcaagcc
     6361 gctcaagccc aacgcgctcg ccgattgcgg ctggggacgg gtgctgttcg cgcacacgtt
     6421 cgaggatgtc gccgagcttg tcgccgagat ccgcgccgag gggccggccg agcgcgacat
     6481 cgccctgtac gtgaccgacc cgcacgtgct gatcgcctcg gcgccggccg agctgttcct
     6541 cgacccgtcg catacgtacc ggctcaacct cgccacttac cggccgtggc agcgcaagcc
     6601 gcccggcttc atggtgcggc gcatggtggc cgacaccgac gccgatgcgg tcaaccggct
     6661 ctacgcgctc caccgcatgg tccaggtgcg gcccgatttc ttcgaccagg agagccaggg
     6721 ccggctgatc acctatttcg tcgccgagga cgaggagacc ggggcggtga tcggcaccgt
     6781 caccggcatc gaccatgccg ccgcgttcga cgacccggag catggatcgt cgctgtggac
     6841 gctcgcggtc gacccgcaga ccgcccatgc gggcgtcggc gaggctttga tccgctggct
     6901 cgccgaacat tatatcgcgc gcggcgccgg ctatatggac ctgtccgtac tgcacgacaa
     6961 tcacggtgcg atcgcgcttt acgagaagct cggctttcac cgcctgccgg tgttcgcggt
     7021 caagcgccgc aacgtgatca acgagcggct gttcgccggt cacgaccccg ccaaggacat
     7081 gaacccctat gcgcggatca tcatcaacga ggcgcggcgg cgcaacatcg gggtagacgt
     7141 gatcgacgcc gccggcgggc tgttccagct ccattcgggc gggcgtagcg tgcgctgccg
     7201 cgagagcctg tccgagctga ccagcgcggt ggccttgtcg atctgcgacg acaaagcgat
     7261 gactcggcgc atcgtcgagg cggccggcgt gacggtgccg gaggaggtcg aagcctccga
     7321 tggccaggcg gtgcgggaca tgctcgccaa gcacgggtcg ctggtggtga agcccgcgcg
     7381 cggcgagcag ggccgcggcg tcgccgtcga tctcgacacg atcgagagcg tggaccgggc
     7441 gatcgaggcc gcccgcgccc attccgagca ggtgctggtc gaaagcttcc acaagggcga
     7501 ggatctgcgc ctgatcgtga tcggcttccg gctggtcgcc gcggcggtgc gaaggccggc
     7561 cgaagtggtc ggcaacggcc gcgacagcgt gcgtcagctg atcgagcggc agagccggcg
     7621 gcgcgcggcg gccacgggcg gcgaatcgcg catcccgctc gatgccgaga ccgagcgctg
     7681 cgtcgccaag gccggctttg cgatggacga ggtgccgccc gagggcgcga ccttcccggt
     7741 gcgcaaggcc gcgaacctcc acaccggcgg caccatccac gacgtcaccg cttccctcga
     7801 tccgcagctc gccgaggcgg cgatccgagt ggcgcgggcg atcgacatac cggtggtcgg
     7861 ggtcgacttc atcgtgacga gccccgacca gcccgattat gtgttcatcg aagccaacga
     7921 gcggccgggg ctcgccaatc acgagcctca acccaccgcc gagcggttca tcgacctgct
     7981 ctttcccctg agcatgccgg cggcgcgcca tcaccatgag gggggttaag ggcttggagt
     8041 taggtctgtg tctcggctgc gctcgacggg gtgtctcgac ttcgctcgac acgaacgggt
     8101 ggagggcgtg atggatagga tgagccaggg aagcgaaggt gcgagtcgag tgactgggcc
     8161 tcagatcgat accgattatc tgaagacgca gctcgaccgg ctgctgtcga tcccgagccc
     8221 gaccggctat accgacacgg tggtgcgcca cgtgtcgcag gagctggagc ggctcggcgt
     8281 caactattcg gtgacccgcc gcggcgcgat cctcgccctc taccagggcg ccgaggtcga
     8341 gggtgcgcgc gcgatcgtgt cgcacctcga tacgctcggc gcgaccgtca agatgatcaa
     8401 ggacaatggg cggctcgagc tggtcccgat cggcacctgg tcggcgcggt cggccgagag
     8461 cagccgctgc accatcttca ccgaggaagg gtcgtatcgc ggcaccatcc tgccgctcaa
     8521 ggcctcgggc catacgttca acgaggaggt cgacacccag ccggtcggct ggcgccatgt
     8581 cgaggtgcgg atcgacgccg actgcaccga ccgcaagagc gccgaggcgc tcggcatcca
     8641 tatcggcgat ttcgtggcga tcgataccgg caccgaattc ctcgacaacg gctatatcgt
     8701 gtcgcgtcac ctcgacgaca aagccggctg cgcagtggcg ctggcggcgc tgaaagccct
     8761 ggtcgacggc aatgtcgcga cgccggtcga catccatttc atcttcacca tcaccgagga
     8821 ggtcggatcg ggcgcctcgg cggtgctgct cgacaatgtc gtggcgatgg tgtcgatcga
     8881 caacggcacc accgcgcccg gccagaacag ctccgaattc ggagtgacga tcggcatggc
     8941 cgaccaggtc gggccgttcg attatcatct gacgcgcaag ctcatccggc tgtgccagga
     9001 aaacgagata cggcaccagc gcgacgtgtt ccgctattac cggtcggaca gcgcctcggc
     9061 gctggagggg ggcgcggacg tgcgcaccgc gctcatcacc ttcggcatcg acgccagcca
     9121 cggctatgag cggatccacc tgcattcgct gcgctcgctg gcggagctgg tcgtgctcta
     9181 tgcggggagc ccggtcgaga tcgcccgcga cgtcgaggaa ctggccggga tcgccggctt
     9241 caccgagcag ccgaccgagc ccgcgcggca agccgaggac gatccgtcga tgcacgagtg
     9301 agggcgcggt tgtgtggccc gtgggccgtc accccggact tgatccgggg tctgccttct
     9361 tcatcccgcg cccgaggaaa aaggcgggtg ccggatcaag tccggcatga cgagatgggc
     9421 gagacgggct cgttagcgcc ttagacgaag caggcgggat cgccggcggc ggtgagttgg
     9481 ttggcgggcg cctcgtcctg ctcgacgatc tgaagcggct cggcatcgtc gtcggcggtg
     9541 aggccgatcc ggccgagctg gaaatgggcg acgccgcaga gccggcgcca gtcgagcacg
     9601 cgcagctgaa cgccgtcgct ggcggtcacc gccgagagtt cgagcaaggc gagcgtgcgc
     9661 ttgacccggc acaggctgac gccgagcgcg gtcgcaagtt cgccgcgggt cagcggaagc
     9721 gtgtcgcggt cctcgccgcg gcgggcgagc tgatagccga gctcgcagag cagataggcg
     9781 atccgcccgc gggtgttgcg gcgggtcatg tcggcccgct cggcggccac cgaagcggcg
     9841 agagccgagc tgaaactcca gccgcggtca gccacttgcg gccgcgcgaa caggaacggc
     9901 agcggcgcca ccgagggcgg gggagaggca ggtgcgaact gggtcgccat cggttttcga
     9961 actccggatg cgggccgcga agcgcggctt tcgtatccag ttcaaccgtt tagggcctgc
    10021 cgccgttcct taaaatctga ttaacaaggg ttagcggccg ttccgccccc gatcagatcc
    10081 ggtcgggata atcgggctgg atatcggtcg gaatccgggc ggttgcggcg agcaccgccc
    10141 gtgcctggtc gtcgagtttg gcgtagcggt cgaagaaggc cttggtgccg gcatagtcgc
    10201 cattgccctg caggcggacc agcaaagcgg tgagctcccc gaggccgcgc tcgagcgcat
    10261 tgtaatcgac ccggaacagc cgccgctccg gatcccaggc gaaggcgccg cgctccttca
    10321 gccagctata ttgcatcgcc gcgccgcgcc cgtgcgcctc atcgatgccg aagcggacgg
    10381 cgcggaagat gccggcgaaa taggtcgcca gcgcctgctc gcgctcggcc gcgggaagct
    10441 cgccgcgctt catcatatag aggatgttgt gcatccccat cacgtccgcc ttggcttcct
    10501 cgctcggcga atagacgtcc ttcagctcgg cattgaccgt cgtcttgcgg ccgccgaccg
    10561 cgatcgtgcc gggcccgagg ctgtgcgaca gctcgtggaa cagggtctcg ttggtcatat
    10621 aggcacggct gaccagccgc gcctgatccg gcaccagcac atgctgcgcc atcggcccga
    10681 ggatgcggtc atatttggcg ccgagcacgt tggcgaggat caccttcttg gcgcccttgg
    10741 cctcgcgcac gcgctcgtca ttgggaaggt tgaaggcgac cgtctgcacg cccggcacgg
    10801 agtcgccgcc ggcatggacc tggtcggcga ccaggatcgg cgaggcgaag gggcggttga
    10861 aattcttgta tttctcctcg accgggaggt tcgcctccat gtcgcgcagc aggcccttat
    10921 atttctggag cgcgccgctc tccttcgggt cctggacggt gacgaaggat tcgaaggcgg
    10981 tcttggtgcc gtagagcgag tcggtgtaga cctcataggg gccgatcacg acttcgatcg
    11041 gggtgccggc gacgtccatc caggcgagct cggacggata ataatcgttg ctgcgaaagg
    11101 cctgggcgcg gagcgtcagg aacttgccta agctggcatt gctggtcgtc t
//
LOCUS       DASVRK010000072         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_137560, whole genome shotgun sequence.
ACCESSION   DASVRK010000072 DASVRK010000000
VERSION     DASVRK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137560"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VF589_02495"
     CDS             <1..841
                     /locus_tag="VF589_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256478.1"
                     /translation="EGEDDIRIDATASGLTAALGWGVVRIDHVDEGEQLIIAAHGGDD
                     RLDASAVDRQVLELALDGGAGDDLLVSGFGDETLLGGEGSDTIDYRASDGNVAVDLGA
                     ERAAGGYAQGDVISSIENVNGSGFSDVLTGSSETNVIKGGGGDDQIFGITGNDAGNLI
                     AGGGGNDVLRGLGGSDAFLFDTALIGAGNVDRILDFSSDNDIIRLDNDVFEGLAEGAL
                     AAGAVRAGRTALQADDRILYDQASGALFYDADGSGDGAAAVRFATLQPGAELSAAEFL
                     VVA"
     gene            complement(838..>1653)
                     /locus_tag="VF589_02500"
     CDS             complement(838..>1653)
                     /locus_tag="VF589_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256479.1"
                     /translation="PLNTFDALVPKDSKAAVVASDQPFMSGKRGKLDVYAPRGTNASS
                     NLPVIVFFYGGSWNSGVKEGYGFAGRALAAQGFLVAIPDYRLVPEVRFPGFLEDSAAA
                     VRWVRANAARFGGDPDRIVLAGHSAGAYNAAMLSLDPRWLGADRAAVKGFAGLAGPYS
                     FLPLDVDSTRAAFGAAPDLQATQPIRFASAGDPPALLLAGSADTLVRPAQAAALAAAL
                     RRVGASAETRLYPGIGHIGIVTALARPWRGKAPVLADVGEFARRVTAPAPAAP"
BASE COUNT          253 a          535 c          631 g          234 t
ORIGIN      
        1 cgagggtgaa gacgacatcc gcatcgatgc caccgcgtcg ggactgacgg cggcgctcgg
       61 ctggggcgtg gtccggatcg accatgtcga tgagggcgag cagcttatca tcgccgccca
      121 cgggggcgac gaccggctcg atgcctcagc cgtcgatcgc caggtgctgg agctggctct
      181 ggacggcggc gctggcgatg acctcctggt ctcgggcttc ggcgacgaga ctttgctggg
      241 cggcgagggt agcgacacga tcgactatcg cgcttcggac ggcaatgttg cggtggacct
      301 cggcgccgag cgagcggcgg gaggctacgc tcagggagac gtcatttcca gcattgagaa
      361 tgtgaacgga tcgggcttca gcgacgtgct gacggggtct agcgagacca atgtcatcaa
      421 aggaggcggc ggcgacgatc agatcttcgg catcaccggc aacgatgccg gcaacttgat
      481 cgccggcggc ggcggcaacg acgtgctgcg cgggctcggc ggcagtgatg cctttctgtt
      541 cgacaccgcg ctgatcggcg cgggcaatgt cgaccggatc ctcgacttct cgtccgacaa
      601 cgacatcatc cggctcgata acgacgtgtt cgaggggctg gccgaaggcg cgctcgcggc
      661 gggggccgtc cgtgccggcc ggaccgcgct ccaggcggac gaccgcattc tctacgacca
      721 agcgagcggc gccttgttct acgatgccga cggctcgggc gacggagcgg cggcggtgcg
      781 gttcgcgacg cttcagccgg gcgccgagct cagcgccgcc gaattcctcg tcgtggccta
      841 aggcgcggcc ggcgccggcg cggtgacccg gcgggcgaat tcgccgacgt cggcgagcac
      901 cggcgccttg ccgcgccagg ggcgggcgag ggcggtgacg atgccgatgt ggccgatgcc
      961 gggatagagg cgggtctccg cgctcgcccc aactcgccgg agcgcggcgg cgagggcggc
     1021 ggcctgcgcc ggccggacca atgtgtccgc gctcccggcc agcagcaagg cgggcggatc
     1081 tccggcgctg gcgaagcgga tcggctgggt cgcctggaga tcgggcgccg cgccgaaggc
     1141 ggcgcgggtc gagtccacgt cgagcggcag gaagctgtag gggccggcga gcccggcaaa
     1201 gcccttcacc gccgcccggt cggcgccgag ccagcgcggg tcgaggctca gcatggcggc
     1261 attgtaggcg ccggcgctgt ggccggcgag gacgatgcgg tcgggatcgc cgccgaagcg
     1321 ggcggcattg gcgcgcaccc agcgcaccgc cgccgcgctg tcctcgagga agccggggaa
     1381 gcggacctcg ggcaccagcc ggtaatcggg gatggcgacg aggaagccct gggcggcgag
     1441 cgcgcggccg gcaaagccat agccttcctt gaccccgctg ttccacgagc ctccgtagaa
     1501 gaacacgatg accggcaggt ttgacgaagc attggtcccg cgcggagcgt agacgtcgag
     1561 cttcccacgc tttcccgaca tgaagggctg atcgctcgcc accactgcgg ctttgctgtc
     1621 cttgggcaca agcgcgtcga acgtgttgag cgg
//
LOCUS       DASVRK010000073         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_262152, whole genome shotgun sequence.
ACCESSION   DASVRK010000073 DASVRK010000000
VERSION     DASVRK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262152"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="VF589_02505"
     CDS             complement(<1..1684)
                     /locus_tag="VF589_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8256480.1"
                     /translation="MADQALLAPDARTNAPDADFDWRRVVYLTLVSRALDKLEETRLV
                     PEKKVLYQFSARGHDMAQVMLGLKLDRPKDAACGYYRSRPLLLALGVPLEDALGSNMG
                     RAGGYSDGRDIGVVFNYPNPNGAPALPMSGGVGAQYTPTAGWAQAIEYHRTVLDDPVY
                     EGAMAVVLGGDASVAANGFWAALTIATTQKLPMLFYIEDNGYGISVPSSYQTPGGNIA
                     DNLASFKNLAIFSGDGTEPAEAARLMKLALAHVRGRKGPAMLRLTVPRLQGHSFQDTQ
                     TYKSEDFVASEWSRDPLPKLRSYLVGSLLSEADWAGIEAEAEAAVETARAAAEARGVA
                     DPETLLDNVFYQGAIQAIGGQWSHGYEPPPGHDDPRPEGQRINMVTAIRRTLDRELEI
                     NPRVLLFGEDIGPKGGVHAVTLGLQDKYGAARVFDTSLSEEGIVGRAVGMALAGLMPV
                     PEIQFRKYAEPATEQINDCGTMRWRTNNRFAAPMVLRVPGGFFKCGDPWHSQTNEVQF
                     VHKPGWLVAVPSNAEDAVGLLRTALRGNDPVIFFEHRSMLDDPWARRPYPGDD"
     gene            complement(1677..>1958)
                     /locus_tag="VF589_02510"
     CDS             complement(1677..>1958)
                     /locus_tag="VF589_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8256481.1"
                     /translation="ADWHAELQEMCGRIRSLRARLAGYDERLAYIGEQNGMFSMLPLS
                     GEAVLELRRSHGIYMAGSGRFNIVGLSDENVDRFAAAVVASMNGVNADG"
BASE COUNT          312 a          678 c          646 g          322 t
ORIGIN      
        1 aatcgtcgcc aggatagggc cgccgagccc aggggtcgtc gagcatcgag cgatgctcaa
       61 agaagatgac gggatcattg ccgcgcagcg cggtgcggag caggcctacc gcgtcctcgg
      121 cgttggaggg caccgccacc agccagcccg gtttgtggac gaactgcact tcgttggtct
      181 ggctgtgcca cgggtcgccg catttgaaaa agccgccggg cacgcgcagc accatcggcg
      241 cggcgaagcg gttgttggtg cgccagcgca tcgtcccgca atcgttgatc tgctcggtcg
      301 ccggctcggc atatttgcgg aactggattt ccggcaccgg catcaggccg gccagcgcca
      361 tccccaccgc gcggcccaca atgccttcct ccgacagact ggtgtcgaac acgcgggcgg
      421 cgccatattt gtcctggagg ccgagcgtca cggcatggac gccccccttg gggccgatat
      481 cctcgccgaa cagcagcacg cgcgggttga tctcgagctc gcggtcaagc gtgcggcgga
      541 tcgccgtcac catgttgatc ctctgcccct cgggccgggg atcgtcatgg ccgggcggcg
      601 gctcatagcc gtggctccat tgcccgccga tcgcctggat cgcgccctgg tagaagacgt
      661 tgtcgagcaa tgtttcggga tcggccacgc ccctcgcctc ggccgcggcg cgcgccgtct
      721 ccaccgccgc ttcggcttcg gcctcgatcc cggcccaatc ggcttcggac agcagcgagc
      781 cgaccagata agatctaagc ttcggcagcg ggtcgcgcga ccattcggag gcgacgaaat
      841 cctcggactt ataggtctgg gtgtcctgga agctgtgccc ctggagccgc ggcacggtca
      901 gccgcagcat cgccgggccc ttgcggccgc ggacatgggc gagcgcgagc ttcatcagcc
      961 gcgccgcctc ggcgggctcg gtgccgtcgc ccgagaagat ggcgagattc ttgaagctgg
     1021 cgaggttgtc ggcgatgttg ccgccgggcg tctggtagct cgacgggacc gaaatgccgt
     1081 agccattgtc ttcgatgtag aagagcatcg gcagcttctg cgtggtggcg atggtcagcg
     1141 ccgcccagaa gccgttcgcc gccaccgaag catcgccgcc gagcacgacc gccatcgcgc
     1201 cttcatagac gggatcatcg agcaccgtcc gatgatattc gatcgcctgc gcccagcccg
     1261 ccgtcggcgt atattgcgcc cccacgcctc ccgacatcgg cagcgccggc gccccgttag
     1321 gattgggata gttgaacacc accccgatat cgcggccgtc cgaatagccc ccggcccggc
     1381 ccatgttgga accgagcgcg tcctcgagcg gcacgccgag cgccagcagc agcggccgcg
     1441 agcgataata gccgcaggcg gcgtccttcg gtcggtcgag cttcaggccg agcatgacct
     1501 gcgccatgtc gtgcccgcgc gcggagaatt ggtagagcac cttcttctcg gggacgagcc
     1561 gcgtctcctc gagcttgtcg agcgcgcggc tgaccagggt cagatagacg acccggcgcc
     1621 agtcgaagtc cgcatcgggc gcgttggtcc gcgcgtccgg cgccagcaag gcctggtcag
     1681 ccatcggcat tcactccgtt catgctcgcc acgaccgcgg cggcgaagcg gtcgacattc
     1741 tcgtccgaca gcccgacgat gttgaagcgc cccgatcccg ccatgtagat gccgtggctg
     1801 cggcgaagct cgagcaccgc ctccccgctc agcggaagca tcgagaacat gccgttctgt
     1861 tcgccgatat aggccagccg ctcgtcatag ccggcgagcc gcgcgcgcag cgagcggatg
     1921 cggccgcaca tctcctggag ttcggcatgc cagtcggc
//
LOCUS       DASVRK010000074         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_323789, whole genome shotgun sequence.
ACCESSION   DASVRK010000074 DASVRK010000000
VERSION     DASVRK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323789"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VF589_02515"
     CDS             complement(<1..445)
                     /locus_tag="VF589_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256482.1"
                     /translation="MLRFTPEDHALVTAAVTAAERASDGEIVTIVTPESDSYHDVGLH
                     YAVLAMLLVPAALAAVPQSLIDWATAPLLGWNEALSRGMLMAFLFMKLAAVFLIVRYG
                     LAWIPLRMALTPGSTKSRRVRRRAVELFKASAEHRTHGRTGVLLYL"
     gene            complement(445..1248)
                     /locus_tag="VF589_02520"
     CDS             complement(445..1248)
                     /locus_tag="VF589_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX8256483.1"
                     /translation="MILRRLLLLLLMMIAAAPASAQQFPKLSGRVVDAANLLSPAEEA
                     AITGKLEGLERASSRQLVVATIPDLQGYPIEDYGYRLGRAWGIGQTEANNGTILIVAP
                     RDRKVRIEVGYGLEPILTDALSSLIVQNQILPRFRANDFPGGINAGVDSIVQQLQAPP
                     ELAEQRALAAAAEQGEQASGGEGGSIFPLLFWGAVLFFIIIPMFRGRGRGRRHRRGGF
                     PVVIWGPGFGGGGGGGFGGFGGGGGGGGGFGGFSGGGGSFGGGGASGGW"
     gene            complement(1245..>1538)
                     /locus_tag="VF589_02525"
     CDS             complement(1245..>1538)
                     /locus_tag="VF589_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX8256484.1"
                     /translation="HWGIGSKAQSDGLLLIVAPSERKVRIEVGYGLEKRISDPFAAQV
                     IREQALPRFREGRFPEGIEAASEALMKRLLPPRPDKDAAPAGAEPAAAEKVPA"
BASE COUNT          245 a          572 c          517 g          204 t
ORIGIN      
        1 acagatagag cagcacaccg gtgcggccgt gggtgcggtg ctcggcgctc gccttgaaca
       61 gctcgacggc gcggcgccgg acgcggcgcg acttggtcga accgggggtg agcgccatcc
      121 ggagcggaat ccaggcgagc ccgtagcgga cgatcaggaa caccgccgcc agcttcatga
      181 acaggaacgc catcagcatg ccgcggctga gcgcctcgtt ccagccgagc aacggcgccg
      241 tcgcccagtc gatcagcgac tgcggaaccg ccgccagcgc ggccggcacc agcagcatcg
      301 ccagcaccgc ataatggagc ccgacatcgt gatagctgtc cgattcgggc gtgacgatgg
      361 tgacgatctc gccgtcgctg gcccgctccg ccgcggtgac ggcggcggtg accagcgcgt
      421 ggtcctcggg cgtgaagcga agcatcacca gccccccgac gcgccgccgc cgccgaacga
      481 gccgccgccg cccgaaaagc cgccaaagcc gccgccgcct cccccgccgc ccccgaagcc
      541 gccgaaaccg ccgcctccgc cgccgccgaa gccgggaccc cagatcacga ccgggaagcc
      601 gccgcggcga tggcgccggc cgcggccgcg gccgcggaac atcgggatga tgatgaagaa
      661 caataccgcg ccccagaaca gcagcgggaa gatcgatccg ccctcgcccc cgctcgcctg
      721 ctccccctgc tcggccgcgg cggcgagcgc gcgctgctcg gcaagctccg gcggcgcctg
      781 cagctgctga acgatgctgt cgacgccggc attgatcccg ccgggaaagt cgttggcgcg
      841 gaagcgcggc aggatctggt tctggacgat caggctggaa agcgcgtcgg tcaggatcgg
      901 ctcgagcccg tagccgacct cgatcctcac cttgcggtcg cggggcgcga cgatcaggat
      961 ggtgccgtta ttggcctcgg tctggccgat cccccaggcg cggccgagcc ggtagccata
     1021 atcctcgatc ggatagccct gaaggtcggg gatcgtcgcc accaccagct gccggctgct
     1081 cgcccgctcc agcccctcga gcttgccggt gatcgccgcc tcttcggccg gcgacagcag
     1141 gttcgccgcg tcgaccacgc gtccgctgag cttcgggaat tgctgcgcgg aagcgggcgc
     1201 cgccgcgatc atcatcagca gcagtaggag caggcgccgc aagatcatgc cggcaccttc
     1261 tcggcagcgg cgggctcagc gccggcgggc gctgcatcct tgtcaggccg gggcgggagc
     1321 agtcgcttca tcagcgcctc gctagccgct tctatgcctt cggggaagcg gccctcccgg
     1381 aaacgcggca gcgcttgctc acggattacc tgcgcggcga agggatccga gatgcgcttc
     1441 tcaagcccgt agcccacctc gatccggacc ttccgctcgc tgggcgcgac gatcagcagc
     1501 agcccgtcgc tctgggcctt gctgccgatc ccccaatg
//
LOCUS       DASVRK010000075         5553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_138120, whole genome shotgun sequence.
ACCESSION   DASVRK010000075 DASVRK010000000
VERSION     DASVRK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138120"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF589_02530"
     CDS             <1..187
                     /locus_tag="VF589_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX8256485.1"
                     /translation="VATRRSGSNELLLQEMLQLCVTACDICAEECRRHDHEHCRLCAE
                     MCEETARDCRAALETFH"
     gene            complement(666..1229)
                     /gene="def"
                     /locus_tag="VF589_02535"
     CDS             complement(666..1229)
                     /gene="def"
                     /locus_tag="VF589_02535"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX8256486.1"
                     /translation="MSLLPIIEIPDPILRQASLPVETVGEDISRLVRDMLETMYAAPG
                     IGLAAVQVAVPRRLLVIDLQDPDPDAEVPEGEEPPPVRRPHVFINPEIVHRSDARKTY
                     NEGCLSIPDQYAEVERPDIVRARWIDETGAAREGEFGGLMSVCLQHEVDHLNGVLFID
                     YLSRLKRDMVVKKVLKARKDRDKAAIL"
     gene            complement(1339..1935)
                     /gene="recR"
                     /locus_tag="VF589_02540"
     CDS             complement(1339..1935)
                     /gene="recR"
                     /locus_tag="VF589_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX8256487.1"
                     /translation="MASPEIDALTQALARLPGLGPRSARRAVLHLIKKRETAMAPLLV
                     ALERVNQRLAVCGACGNVDTIDPCTICADPRRDQRLLCVVEEVSDLWALDRSRLFPGR
                     FHVLGGRLSALEGIRPEDLAIDTLVGRVAAGGIDEVVLAMNATLEGQTTAHYLAERLE
                     TYPIRLTQLAHGLPVGGELDYLDEGTLAQALRARRPVS"
     gene            2068..3006
                     /gene="fmt"
                     /locus_tag="VF589_02545"
     CDS             2068..3006
                     /gene="fmt"
                     /locus_tag="VF589_02545"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX8256488.1"
                     /translation="MRLAFMGTPDFAVPTLDALLAAGHEVAAVYSQPPRPAGRGKALR
                     PSPVQHRAEAAGIEVRTPLTLRDPAAQAAFAALDLDAAVVAAYGLILPAPVLEAPRLG
                     CLNVHGSILPRWRGAAPVQRAILAGDAMTGVTIMQMERGLDTGPMYRVRETAVDGKTA
                     GELTAELAAIGAALMVEVLKELPGLEPVPQPEEGVTYAAKVEKHEARLDFARAAVEVE
                     RQVRAFNPVPGGYFEFGGERIKVLEAAVLAGVRRVPAALESGPRPSPGSVDGDLAIAC
                     GSGALVPTLVQRAGRGAMTPGELLRGFPIPVGTRLG"
     gene            3003..3767
                     /gene="truA"
                     /locus_tag="VF589_02550"
     CDS             3003..3767
                     /gene="truA"
                     /locus_tag="VF589_02550"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX8256489.1"
                     /translation="MTRFRLTVEFDGRPFMGWQRQPHGPSVQQAIEEAVTAITGEEAA
                     VHGAGRTDAGVHALAMAAHVDIVKPITPFRLADALNAKLRPRPVAILKAEMVADDWHA
                     RFSCTGRRYLYRIANRRAPLALEAGRAWRVPVVLHADAMHKAAQYLVGRHDFTTFRSA
                     HCQAESPVRTLDLLTVRRFGETIEIEAAARSFLHHQVRSMVGCLELVGRGKWTAKDLK
                     AALAARDRAALGFNAPPDGLYFAAALYDETPRPKIR"
     gene            complement(3716..>5553)
                     /locus_tag="VF589_02555"
     CDS             complement(3716..>5553)
                     /locus_tag="VF589_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HEX8256490.1"
                     /translation="RGPDGSGVWTAPGVGFGHRRLAIIDLTPGGAQPMLTPDERVAIT
                     FNGEIYNFHEVRAELEGRGHVFRTASDTEVILAAWRQWGPECLSRLNGMFALALYDAD
                     ADALFLARDRLGVKPLHYAELPDGGLVFASELKGLLAHPLMRRGISPQAVDDYFAFGY
                     VPDDASIVSGVKKLAAGHYLLVRRGMPMPQPVRWWDVDFSSPTRASAADLEAELIDHM
                     RQAVRSRMVADVPLGAFLSGGVDSSAVVALMAEASRSAVETCSIGFDSPDHDESLYAA
                     MVAERFATRHRSRIVAADDFGLIDTLAAAFDEPFADASALATYRVSELAREKVTVALS
                     GDGADEAFAGYRRYRLFAGEERVRRLLPGAAGRAIGALGDVYPKLDRAPQFLRAKTTL
                     QALGKSSGEAYAEAVGVTPPALRARLYGENLRTALQGHRPEDRYVKAMAEAPAADALS
                     RAQYADLKIWLPGDILTKVDRTSMAVSLEAREPLLDYRLIEFAARVPARMRLRGGSGK
                     WLMKKALGRYLPDEILHRRKMGFVTPISAWFRGPLAGEAAAIARGSALAELGWFDPAA
                     IGRIAADHRAGKADHGRLLWQLLMLDKSLQRIFGLGVSSYSAAAK"
BASE COUNT          789 a         1756 c         2179 g          829 t
ORIGIN      
        1 ggtcgcgacg cggcggagcg ggagcaacga attgctgctc caggagatgc tgcagctctg
       61 cgtcaccgcc tgcgacatct gcgccgagga gtgccggcgc cacgaccatg agcattgccg
      121 gctgtgcgcc gagatgtgcg aggagaccgc gcgcgactgc cgggccgcgc tggagacctt
      181 ccactaggtc agcggccatt aagagcattg acgggtccag acccgcgcga tgcctgagag
      241 cgcttggctg gcggcccctt ccttcgcagg ggccgggctg ggcccctgcc ttcgcagggg
      301 cacgggaacg gcaaacccga gcctgcgcca cgtgcccctg cgaaggcagg ggcccagacc
      361 cgggttgccg cttcagcggc agcccggtca tcgcagcgtc gctggaagct ttggcgccgt
      421 agcccacacg gtcccggccc cgcgcgaatg tctacgagag tttgcctggc gggctttcgc
      481 ccggtctggg cccctgcctt cgcaggggca cgggtgcggc gcctgtcgca gcggctgtgg
      541 aagcctggcg ccggagcccg cacggccccc cttcgacgcc gcctgcggcg tcgctcagga
      601 caggcttcgg caggctcagg gggaacggat gatggtggtt ccgttcgagc cttcgacgct
      661 ccagctcaga ggatggcggc tttatcgcgg tctttccggg ccttcaggac cttctttacg
      721 accatgtcgc gcttcaggcg cgagagatag tcgatgaata ggacgccgtt caaatggtcg
      781 acctcatgct ggaggcagac cgacatcagg ccgccaaact cgccctcgcg ggcggcgccg
      841 gtctcgtcga tccagcgggc gcggacgatg tcgggccgct cgacctcggc atattgatcg
      901 gggatggaga ggcagccttc attgtaggtc ttgcgggcgt cggagcggtg gacgatctcc
      961 ggattgatga agacgtgggg gcggcggacc ggcggcggct cctccccctc cggcacttcg
     1021 gcgtcgggat cgggatcctg gaggtcgatg acgagcaggc ggcgcggcac ggcgacctgg
     1081 accgcggcga ggccgatgcc gggggcggcg tacatggtct cgagcatgtc gcggacgagg
     1141 cggcttatgt cctcgccgac ggtttcgacc ggcagcgagg cctggcggag gattgggtcg
     1201 gggatttcga tgatcgggag cagggacatg gcggcccgct atggaagcgg tgggcgggcg
     1261 tcaagtcggg cgcttcgcgc cgcccctcca ccatgctgcg catggtcccc ctcccccgca
     1321 aaggcggggg aggaattttt aggagacggg gcgtcgggcc cttagggcct gggcgagggt
     1381 gccttcgtcg agatagtcga gctcgccgcc gaccggcagg ccgtgggcga gttgggtgag
     1441 gcggatgggg taggtttcga gccgctcggc gagataatgg gcggtggtct ggccttcgag
     1501 ggtggcgttc atcgccagca cgacctcgtc gatgccgccg gcggcgacgc ggccgaccaa
     1561 ggtgtcgatg gcgagatcct ccggccttat tccctccagc gctgagagcc ggccgccaag
     1621 cacatggaaa cggccgggaa acaggcgcga gcggtcgagt gcccagaggt ccgacacctc
     1681 ctcgactacg cagagcagcc gctggtcgcg gcggggatcg gcgcagatgg tgcaggggtc
     1741 gatggtgtcg acattgccgc aggcgccgca gacggcgagc cgctggttga cccgctcgag
     1801 cgcgaccagc agcggcgcca tggcggtctc gcgcttcttg atgaggtgca gcaccgcgcg
     1861 gcgcgccgag cgcgggccga ggccgggcag ccgcgccagc gcctgggtga gcgcgtcgat
     1921 ttcgggtgag gccatggcgg ggatgtagca aatccctccg ggcacgggga gggcccttgc
     1981 agcaccccgc gggtcgcggg gccccctaca ccgcacttcg tgcggtcccc ctccccgttc
     2041 cggggaggat aagctagggg acgtctcatg cgcctcgcct tcatgggaac gccggatttc
     2101 gcggtgccga cgctcgatgc cttgctggcg gcggggcatg aggtggcggc cgtctattcg
     2161 cagccgcccc ggccggccgg gcgcggcaag gcgttgcgtc catcgcccgt gcagcaccgg
     2221 gcggaagccg ccgggatcga ggtgcgcacg ccgctcacgc tgcgggatcc tgcggcacag
     2281 gccgcgtttg ccgcgctcga cctcgatgcc gcggtggtgg cggcttacgg gctgatcctg
     2341 ccggcgccgg tcctggaggc gccgcggctg ggctgcctca acgtccatgg ctcgatcctg
     2401 ccgcgctggc gcggggcggc gccggtgcag cgggcgatcc tggccggcga tgcgatgacg
     2461 ggcgtcacca tcatgcagat ggagcgcggg ctcgacacgg ggccgatgta ccgggtgcgc
     2521 gagacggcgg tggacggcaa gacggccggc gagctgacgg cggagctggc ggcgatcggg
     2581 gcggccttga tggtcgaggt gctgaaggaa ttgccggggc ttgagccggt gccgcagccg
     2641 gaagagggcg tcacttatgc ggcgaaggtc gagaagcatg aggcgcggct ggatttcgcg
     2701 cgggcggcgg tggaggtcga gcggcaggtg cgggcgttca atccggtgcc gggggggtat
     2761 ttcgaatttg ggggtgagag gatcaaggtg ctggaagcgg cggtgctcgc cggggtacgc
     2821 cgggttccag ctgcgctgga gtctgggccc cggccttcgc cggggagcgt ggacggggat
     2881 ctcgccatcg cctgcggctc gggtgctctc gtccccaccc tcgtccagcg cgcggggcgc
     2941 ggcgccatga cgccgggcga gttgctgcgg gggttcccca tccccgtcgg tacacggctc
     3001 ggatgacgcg gtttcggctg acggtggaat ttgacgggcg gccgttcatg ggttggcagc
     3061 ggcagccgca tgggccgtcg gtgcagcagg cgatcgagga ggcggtcacc gcgatcaccg
     3121 gcgaggaagc ggcggtgcac ggcgccggcc gcaccgatgc cggcgtccac gcgctggcga
     3181 tggcggcgca tgtcgatatc gtgaagccga tcacgccgtt ccggctggcg gacgcgctga
     3241 acgccaagct gcggccgcgg ccggtggcga tcctcaaggc ggaaatggtg gccgacgact
     3301 ggcacgcccg cttcagctgc accgggcggc gctatctcta ccggatcgcc aaccggcggg
     3361 cgccgctcgc gctcgaggcg gggcgggcgt ggcgggtgcc ggtggtgctc cacgccgatg
     3421 cgatgcacaa ggcggcgcaa tatctggttg ggcggcacga tttcaccacc ttccgctcgg
     3481 cccattgcca ggccgagagc ccggtcagga cgctcgacct gctgacggtg cggcggttcg
     3541 gcgagacgat cgagatcgag gcggcggcgc gctccttcct ccaccatcag gtgcgatcga
     3601 tggtcggctg cctggagctg gtcgggcggg gcaaatggac ggcgaaggat ttgaaggcgg
     3661 cgctggcggc gcgggaccgg gcggcgctcg ggttcaacgc cccgcccgac ggcctctatt
     3721 tcgcggcggc gctatatgac gagacgccga ggccgaagat ccgctgaagc gacttgtcga
     3781 gcatcagcaa ttgccagagc aggcggccat ggtcggcctt gccggcgcgg tggtcggcgg
     3841 cgatgcggcc gatcgcggcc ggatcgaacc agccgagctc ggccagcgcc gagccgcgcg
     3901 cgatcgccgc cgcctcgccg gccagcggtc cgcgaaacca ggctgagatc ggggtgacga
     3961 agcccatctt gcggcggtgg aggatctcgt cgggcagata gcggccgagc gctttcttca
     4021 tcagccattt gccgctgccg ccgcgcaggc gcatccgggc ggggacgcgg gcggcaaatt
     4081 cgatcaggcg atagtctagc agcggctcgc gcgcttcgag gctgaccgcc atgctggtgc
     4141 ggtcgacctt ggtcaggatg tcgccgggga gccagatttt gaggtcggca tattgggcgc
     4201 gggacaaagc gtcggcggcg ggcgcttcgg ccatcgcctt gacgtagcgg tcctcgggcc
     4261 ggtggccctg gagggcggtg cgcagattct cgccgtagag ccgtgcccgc agcgccgggg
     4321 gggttactcc gacggcttcg gcataggcct cgccgctcga tttgccgagc gcctggagcg
     4381 tggtcttggc gcgcaggaac tggggtgcgc ggtcgagctt gggatagacg tcgccgagcg
     4441 cgccgatcgc ccggccggcc gcgcccggga gcaggcggcg gacgcgctcc tcgccggcga
     4501 acaggcggta gcggcggtag ccggcgaagg cctcgtcggc gccgtcgccg gacagcgcca
     4561 cggtgacctt ctcgcgggcg agctcgctga cccggtaggt ggcgagcgcg gaagcatcgg
     4621 cgaagggctc gtcgaaggcg gcggcgaggg tgtcgatcag cccgaaatcg tcggcggcga
     4681 cgatgcggct gcggtggcgg gtggcgaagc gctcggcgac catggcggca taaaggctct
     4741 cgtcatggtc gggactgtcg aagccgatcg agcaggtctc gaccgcgctg cggctcgcct
     4801 cggccatcag ggcgaccacc gccgagctgt cgacgccgcc ggagagaaag gcgccgagcg
     4861 ggacgtcggc gaccatccgc gagcggaccg cctggcgcat atggtcgatc agctcggcct
     4921 cgaggtcggc ggcggaggcg cgcgtggggc tggagaagtc gacgtcccac cagcggaccg
     4981 gctggggcat cggcatgccg cggcggacga ggagataatg gccggcggcg agcttcttga
     5041 cgccgctgac gatcgaggcg tcgtccggca cgtagccgaa ggcgaaataa tcgtcgaccg
     5101 cctgcgggct gatgccgcgg cgcatcagcg ggtgggcgag caggcccttg agttcggagg
     5161 cgaagacgag gccgccgtcg ggcagctcgg cataatgaag cggcttcacg ccgagccggt
     5221 cgcgggcgag gaacaaggcg tcggcatcgg catcgtagag cgccagcgcg aacatgccgt
     5281 tgaggcggga gaggcattcg ggcccccatt gccgccacgc cgccaggatc acttcggtgt
     5341 cgctggcggt gcgaaagacg tggcccctgc cctcgagctc ggcgcggacc tcgtggaaat
     5401 tgtagatctc gccgttgaag gtgatggcga cgcgctcgtc gggcgtgagc atcggctggg
     5461 cgccgccggg ggtgagatcg atgatcgcca ggcggcgatg gccgaagccg acgcctggcg
     5521 cggtccagac gccgctgccg tcggggccgc gat
//
LOCUS       DASVRK010000076         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_138215, whole genome shotgun sequence.
ACCESSION   DASVRK010000076 DASVRK010000000
VERSION     DASVRK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138215"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2634
                     /locus_tag="VF589_02560"
     CDS             <1..2634
                     /locus_tag="VF589_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX8256491.1"
                     /translation="WVRRFDNRFLASLAGGLPQIDTGLSRGPDKILRFHDLRLTAPSI
                     SLAGNGFRRRDGSFWFEGSGRQAESGPFAMSLDGRIERPKMAIRLQRPNEALGLADVL
                     LNLDPTAAGFAYRAAGGSVLGPFSSRGAILLPSGRPTMIEVAALDVSGTGASGLLRAD
                     PGGFTGHLNVAGGGLDGRLLFNPVQGVQRIEAHLGAANARFAGPPPIAIRSGRLDGVI
                     LLNPGATSVEGTLSARGLSRGAMSLASVTAQASMRGGVGRIRADIAGRRGRDFMFRTL
                     AELAPGKIRLSGEGSVDRRPIRLRAPAMLTKIGEDWRLDPAALAFGGGSATVSGLFGP
                     RRTDIDARVEGMPLTVLDIGWPRLGLGGIAAGTLSYRAAPEGGQPSGAANLRVRGLTR
                     AGLVLSSRPVDIGLVAKLAGGNAAMRAVAVSEGRTIGRAQARIAPLGGGGDLVDRLAR
                     APLFAQLRYNGPADTLWRLTGLELLDLSGPIAVGADARGTLNDPQIRGSVRTERARLE
                     SAVTGTVIENIRAAGRFGGSRLVVDSFTGTTKGNGRVAGRGAFDLSASRGFGMDLALR
                     TQNAQIIDRDDIKAQVSGPLAVKSDGSGGLISGRLDLVSGSFRLGSATAAAQVPRLRV
                     RELNLPDDTPEPRRADPWRLDLDVSADNRLRVSGLGMDSEWGADLKLAGTVTQPRIAG
                     RAELVRGNYEFAGRRFRLERGTIRFLGETPINPVLDIVAEGGIQGLNATIRVTGRSQK
                     PEIAFASTPALPQDELLSRLLFGTSITNLSAPEALQLAAAVASLNNPGAGLDPINSVR
                     SAIGLDRLRILPADITTGQGTSIAAGKYLGRRIYVEVITDGRGYSATQLEYQITRWLS
                     LLSSISTVGRESVNVRVSKDY"
     gene            complement(2895..3569)
                     /locus_tag="VF589_02565"
     CDS             complement(2895..3569)
                     /locus_tag="VF589_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8256492.1"
                     /translation="MWQLYQFPLCPFSRKVRLLLAEKGVPHELVRESPWLRRDEFVDM
                     NPAAQTPVLTEAAKGITLIDSNAICEYFEETVEKSPMIPGTAVNRAEVRRLVAWFDDR
                     LYGEVVGPLIEERMRKRLVSRDPPDTKVLREAMKVANTHLDYIDYLLDHRRWLAGPVL
                     SLADLACAAHLSVADYLGGLDWRGHKQTTDWYAVMKSRPSFRPLLGERMEVIAPPAHY
                     DKVDFF"
     gene            complement(3667..>4022)
                     /locus_tag="VF589_02570"
     CDS             complement(3667..>4022)
                     /locus_tag="VF589_02570"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEX8256493.1"
                     /translation="RKADPHYDERMRIFMDVMFAELEKIMDREEPGIRNAMARAYARR
                     FTVEEMRQLDVFFATPIGRKFALQSVMLMEDPELLAQTMSLHPRMQAQMPRVVEKVMA
                     ATAHLPPPPDEEPTS"
BASE COUNT          589 a         1432 c         1397 g          604 t
ORIGIN      
        1 tgggtgcggc gcttcgacaa tcgcttcctc gccagcctcg ccggcgggct gccgcagatc
       61 gacaccggcc tcagccgcgg gccggacaag atcctgcgct tccacgatct gcggctgacg
      121 gcgccgtcga tcagcctcgc cggcaacggc ttccgccgcc gcgacggcag cttctggttc
      181 gaaggctcgg gccggcaggc ggaatctggg ccgttcgcga tgagcctcga cgggcggatc
      241 gagcggccga aaatggcgat ccggctgcag cggccgaacg aggcgctcgg gcttgccgac
      301 gtgctgctca acctcgatcc taccgccgcg ggcttcgcct accgcgccgc cggcggctcg
      361 gtgctggggc cgttcagctc gcgcggggcg atcctgctgc ccagcgggcg gccgaccatg
      421 attgaggtcg cggcgctcga cgtctccggg accggcgcat cggggctgct ccgcgccgat
      481 ccggggggct ttaccgggca tctcaacgtg gccggcggcg ggctcgacgg ccgcctgctg
      541 ttcaaccccg tccagggcgt gcagcggatc gaggcgcatc tgggggccgc caacgcgcgc
      601 ttcgccgggc cgccgccgat cgccatccgc agcggccggc tggacggcgt gatcctgctc
      661 aaccccggcg cgacttcggt cgaaggcacg ctcagcgcgc gcgggctcag ccgcggggcg
      721 atgtcgctcg ccagcgtgac cgcccaggcg agtatgcgcg gcggcgtcgg ccggatccgc
      781 gccgacattg ccggccgccg cggccgcgac ttcatgttcc ggacgctggc cgagctcgcc
      841 cccggcaaaa tccggctgag cggcgaaggc agcgtcgacc ggcgcccgat ccggctgcgc
      901 gcccccgcta tgctcaccaa aatcggcgag gattggcggc tcgaccccgc cgcgctcgcc
      961 ttcggcggcg gcagcgccac cgtctccggc ctgttcggcc ccaggcgcac cgacatcgat
     1021 gcgcgcgtgg agggcatgcc gctgaccgtg ctcgacatcg gctggccgcg gctcgggctc
     1081 ggcggcatcg ccgccggcac gctcagctat cgcgccgcgc ccgaaggcgg gcagccgagc
     1141 ggcgccgcca atctgcgcgt gcgcggcctc acccgggccg gcctcgtcct gtcctcgcgg
     1201 ccggtcgaca tcgggctggt cgccaagctc gccggcggca atgccgcgat gcgggcggtg
     1261 gcggtgagcg aaggccgcac catcggccgc gcgcaggcgc ggatcgcgcc cttgggcggc
     1321 ggcggcgacc tggtcgaccg gctggcgcgc gcgcccttgt tcgcgcagct tcgctataac
     1381 ggacccgccg acacgctctg gcgcctgacc gggctcgaac tgctcgacct cagcgggccg
     1441 atcgcggtcg gcgccgacgc ccgcggcacc ttgaacgacc cgcagatacg gggctcggtc
     1501 cgtaccgagc gcgcccggct cgaaagcgcg gtcaccggca cggtgatcga gaatatccgc
     1561 gccgccgggc gcttcggcgg gtcgcggctg gtggtcgaca gcttcaccgg caccaccaag
     1621 ggcaacggcc gcgtcgccgg ccgcggcgcc ttcgacctgt cggcctcgcg cggcttcggc
     1681 atggatctcg cgctgcgcac ccagaatgcc cagatcatcg accgcgacga catcaaggcg
     1741 caggtgagcg ggccgctggc ggtgaagtcg gacgggtcgg gcgggctcat ctccggccgg
     1801 ctcgacctgg tcagcggcag cttccggctc ggcagcgcga ccgcggcggc gcaggtgccg
     1861 cggctcaggg tgcgcgagct caaccttccc gacgacacgc ccgagccgcg ccgcgccgac
     1921 ccgtggcggc tcgacctcga cgtgtccgcc gacaaccgcc tgcgcgtctc cggcctcggc
     1981 atggacagcg aatggggcgc ggacctgaag ctcgccggca ccgtcaccca gccgcgcatc
     2041 gccggccgcg ccgagctcgt tcgcggcaat tacgaatttg ccggccgtcg cttccggctc
     2101 gagcgcggca ccatccgctt cctcggcgag actccgatca acccggtgct ggacatcgtc
     2161 gccgaaggcg ggatccaggg cctcaacgcc accatccgcg tcaccggccg cagccagaag
     2221 cccgaaatcg ccttcgccag cacgcccgct ttgccgcagg acgagctgct gagccgcctc
     2281 ttgttcggca cctcgatcac caacctttcg gcgccggagg cgctgcagct cgcggccgcg
     2341 gtcgcgtcct tgaacaaccc cggcgccggg ctcgatccaa tcaattcggt gcgcagcgcg
     2401 atcgggctcg accggctgag gatcctgccg gccgacatca ccaccggcca gggcacgtcg
     2461 atcgcggccg gcaagtatct cggccgccgc atctatgtcg aagtgatcac cgacggccgc
     2521 ggctattcgg cgacccagct tgaatatcag atcacgcgct ggctatcctt gctgtcgagc
     2581 atctccaccg tcgggcgcga gagcgtcaac gtgcgggtgt cgaaggatta ttgatccccc
     2641 cgacgaacag ccctcggccg ggtcaggcgg ccgagccgct ccggtcgtag cgcagcagga
     2701 acaggctgcc gccgatgatg aaagcgaata attgcgaaac gatcaaagtt gccatgtcgg
     2761 cccccattcc ggcccatgcc ggatgtcagc caatatgacg caacaaggtt attatccggt
     2821 aaccatatcg ccgcatgttt cggccggacc ggcgcaggtt gcggggctcc ggcgacgttg
     2881 gtgagcgggc gccgctagaa gaagtcgacc ttgtcgtaat gggccggcgg agcgatcact
     2941 tccatccgct cgccgagcaa cgggcggaag ctcggccgcg acttcatcac cgcataccag
     3001 tcggtggtct gcttgtggcc gcgccagtcg agcccgccga gatagtcggc caccgacagg
     3061 tgcgcggcgc aggcgaggtc ggccaggctc agcaccgggc cggcgagcca gcggcggtga
     3121 tcgagcagat agtcgatata gtcgagatgg gtgttggcca ccttcatcgc ctcgcgcagc
     3181 accttggtgt cgggcgggtc gcggctgacg aggcgcttgc gcatccgctc ctcgatcagc
     3241 ggcccgacga cctcgccgta gagccggtcg tcgaaccagg cgaccaggcg gcggacctcg
     3301 gcccggttga cggccgtgcc ggggatcatc ggcgactttt cgaccgtctc ctcgaaatat
     3361 tcgcagatcg cgttcgaatc gatgagggtg atgcccttgg ccgcctcggt cagcaccggc
     3421 gtctgcgcgg ccgggttcat gtcgacgaat tcgtcgcggc gcagccacgg cgattcgcgc
     3481 accagctcgt gcggcacgcc cttctcggcc agcagtagcc ggaccttgcg cgagaaggga
     3541 cagagcggga attggtagag ttgccacatg gatggcgagt catagcgacg gcgcgcgccg
     3601 cgtccacaat ctaagccggc atcgcgggat taatgtgcgg ttcgggccag caccggcggc
     3661 caggcgtcag ctggtcggct cctcgtcggg cgggggagga aggtgcgcgg tggccgccat
     3721 cactttctcc acaacacggg gcatttgcgc ctgcatcctc ggatggagcg acatggtctg
     3781 ggcgagcagc tcgggatcct ccatcagcat gaccgactgg agggcgaact tgcggccgat
     3841 cggggtcgcg aagaagacat cgagctggcg catttcctcg accgtgaagc ggcgggcgta
     3901 agcgcgggcc atggcgttgc ggatcccggg ctcttcccgg tccatgattt tctccagctc
     3961 ggcgaacatc acgtccatga agattcgcat gcgctcgtca taatggggat cggctttccg
     4021 gc
//
LOCUS       DASVRK010000077        11240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_262826, whole genome shotgun sequence.
ACCESSION   DASVRK010000077 DASVRK010000000
VERSION     DASVRK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262826"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VF589_02575"
     CDS             complement(<1..366)
                     /locus_tag="VF589_02575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX8256494.1"
                     /translation="MSSSAPRISPREGIIEEVRAAIGDAFIDAKDAVGEVSITVARES
                     ILEVCRTLRDRFEYQQLMEIAGVDYPQRAERFEVVYHLLSVTKNHRIRLRVHTDDEKP
                     VPSVTGLWPVAGWLEREIFD"
     gene            complement(541..1095)
                     /locus_tag="VF589_02580"
     CDS             complement(541..1095)
                     /locus_tag="VF589_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX8256495.1"
                     /translation="MGVILDPRHENVRPADLQQPDPQYFNELQAEVADKGFLVTSTED
                     LFQWARTGSLWWMTFGLACCAVEMIHVNMPRYDLERFGVAPRASPRQSDVMIVAGTLC
                     NKMAPALRRVYDQMSEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPP
                     TAEALLYGIMQLQRKIRRQGTIER"
     gene            complement(1086..1463)
                     /gene="ndhC"
                     /locus_tag="VF589_02585"
     CDS             complement(1086..1463)
                     /gene="ndhC"
                     /locus_tag="VF589_02585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX8256496.1"
                     /translation="MASTAAEYLPILLFLGVALSLSASFVFLPMIVSRFTGAQNPYPD
                     KLAEYECGFPAFEDSRSQFDVRFYLVAILFIIFDLEAAFLFPWAVSLGAIGIAGWAAM
                     MIFLAELTLGFVYAWKKGALEWE"
     gene            1594..2064
                     /locus_tag="VF589_02590"
     CDS             1594..2064
                     /locus_tag="VF589_02590"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8256497.1"
                     /translation="MAINRVILLASAALLGTPAGAQMVAAKDPQTLVAALQAKGYQAE
                     LGESGGEPRIKSGAGGVAFSIYFDNCTAGAACKTISFVTGFTDVDATLAAVNEWNAKN
                     RFAHAYIDAEQDPMLRMDVDLDHQGIPRANFGEYLDIWASLVPKFLNYLRQDGK"
     gene            complement(2159..2977)
                     /locus_tag="VF589_02595"
     CDS             complement(2159..2977)
                     /locus_tag="VF589_02595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8256498.1"
                     /translation="MVSHSGLITVMQRAARKAAPRIRRDFGEVELLQVSRKGPADFVS
                     LADKRAEQTLVEELRHARPDWGFHMEEGGTVAGDPSKPRWIIDPIDGTSNFLHGIPHF
                     AISIAVEDPRGPGGRPEITQGLVYQPLTDESFWAEKGRGAWLNERRLRVSSRRDLADA
                     LVATGIPYKGHGDHARFEKILAAVAPEVAGIRRFGSAALDLAWVAAGRYDGFWEEDLE
                     YWDIAAGVLLVREAGGFVTDFRGADKALETGQVLASNDQIQTRLHKLVAGALRG"
     gene            complement(3105..3668)
                     /gene="efp"
                     /locus_tag="VF589_02600"
     CDS             complement(3105..3668)
                     /gene="efp"
                     /locus_tag="VF589_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX8256499.1"
                     /translation="MKISGVDIRPGNIIEYEGGIWRAVKIQHTQPGKGGAYMQVEMKN
                     LIDGRKNNVRFRSAETVERVRLDTKDFQFLFADGDSLVFMDKDNYEQVSLPRDLIGDP
                     AAFLQDGMDVVMELWEERPISVQLPDTIEATIVEADAVVKGQTASSSYKPAILENGVR
                     VMVPPHIGSGTRIVVDVYEQTYVRRAD"
     gene            complement(3704..4336)
                     /locus_tag="VF589_02605"
     CDS             complement(3704..4336)
                     /locus_tag="VF589_02605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8256500.1"
                     /translation="MGKLKLILSNLVTAIVVAVVTSAFWIAAYGDEDGRRKTEPAAET
                     AEKRAAAGEAAGDVDIGPSGLAVPVAGIRASDLLDTYTQARAGGSRSHDAIDIMADHG
                     TPVVAAAPGTVEKLFFSKGGGGITVYVRSPDQRHVFYYAHLQDYAPGLKEGQAIRQGQ
                     PIGRVGVTGNSNPDGPHLHFAIHRMAPGEDWHEGRAVNPYPLLAGKRPAR"
     gene            complement(4355..5383)
                     /locus_tag="VF589_02610"
     CDS             complement(4355..5383)
                     /locus_tag="VF589_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8256501.1"
                     /translation="MSKLLPAFLFGSAAVALAGAAVAQQQDDPASQQKPAAAQEKFDG
                     SVFHAQVLLDRAGFSPGVIDGMAGISFEAALKGFQQARGLQATGQLDNSTRATLLRDP
                     APSVRRLRIDESDARGPFTAIPSEPEQQAKMERLAYRNLLEKISEKFHTTPATIIALN
                     NPRMALRAGTVLRLPNVLPTSRNYQDVKPDVAQLLSDLNVSADQPKAAKVVVDKSDKV
                     VHVYAENGRPIAQFPATMGSEKDPLPLGNWKVNSVAFNPPFKYQPQLFWDVKDDEPEQ
                     LLPPGPNGPVGVVWIDLNKEHIGIHGTNSPETIKRAESHGCVRLTNWDAARLTQMIED
                     GIEVVFQA"
     gene            5469..6206
                     /locus_tag="VF589_02615"
     CDS             5469..6206
                     /locus_tag="VF589_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8256502.1"
                     /translation="MTGAIRIGCSGWNYKHWRELFYPKGLPQRLWFEYYSGIFDTVEI
                     NNSFYMLPKAETFAKWRDQAPPGFCYAVKANRFLTQAKKLKDCAEPLERMLTPMRNLG
                     DRLGPILYQLPPRFRVNLERLAEFIDLLPKDLTHVFEFREKSWLTEEVLALLDARGVA
                     FCVHDMPGSATPRWAAGPIAYVRFHGGEGKYWGRYSDEGLLSWIDWIVEQSRAGRPVW
                     CYFNNDIDAHAIHDALTLRAMVGQAAR"
     gene            6343..8580
                     /locus_tag="VF589_02620"
     CDS             6343..8580
                     /locus_tag="VF589_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8256503.1"
                     /translation="MGYSGVFVFGDSLVDAGNALKLAEFAEDISLAELPAGVPTSEKG
                     YYEGRFTDGLTFADLVSNKFISMPTTTVFPFGFTDPWIGVSFRFVSDPEGNNLNFAYG
                     GAQIRKGEEVVPDLDDQTDAFIDAVDGDADPNALHMVTMGGNDVRRLVPDDAPVADAA
                     TARTILADAAQEMHEEVGDLIRAGVRHVVVTGIPDVGMIPEYNGLADEAARRAAATEY
                     SAILDGMIQDYLGQLRVQYPAATIHYVSLTDATTKIVDNLDRIFAPEDLAGLHNPSDV
                     LFFDFVHPTAQAHTLLASAVLDSMSGAPAGERYALTAPDYRVAGNIGQVGEVDTITLS
                     LAANTRYTFELLGLSSGSGSLADPRLQLLGPGNVVFGGDDDGGLGLDASLQFTTGQAG
                     DYVVQMGGVGSLSGGYSFQASGPFQIVHNGTQADDRLVGTNVADRIRGLGGNDLLDGG
                     AGADVMEGGAGNDTYLIDNAGDQVVETSRGGTDHVLASASHRMGAWVETLTLADGALS
                     GTGNSIDNVIVGNAAANSLNGGRGADRLEGGAGNDRYAVDQAGDVVVEAADGGRDVVS
                     SAVSYSLGDNVEDLVLTGAGHTAGTGNSLGNRMSGNGGGNVLKGEAGGDTLRGMDGAD
                     DLYGGDANDVLEGGAGADRFHFDAALNARSNVDRILDFSSADDTIMLDTAIFGNVPES
                     GALSEAAFRQGNAARDADDRIVYNEATGNLYYDADGAGGAAMVLFARVAAGTTLDHLD
                     FAAGL"
     gene            complement(8597..8719)
                     /locus_tag="VF589_02625"
     CDS             complement(8597..8719)
                     /locus_tag="VF589_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256504.1"
                     /translation="MTNAFNTIRARIRSSAVELGLVAALATVAAVTALPALVPA"
     gene            complement(8861..10510)
                     /locus_tag="VF589_02630"
     CDS             complement(8861..10510)
                     /locus_tag="VF589_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256505.1"
                     /translation="MRTLILGATALALAAAMPTQASASAPAQAGRVANLTAEQWRADL
                     AFMAAEMERRHKNLYHEVSRERFAAAVADLHARIPTLRRNQIIVGLMRIAAMVGDGHT
                     RIEPLKDAKFGFPSLPLKLYLFEDGLYVRAAAPAQAGLVGAKVEAIGGVPVAEAIRRA
                     AEISAAENAIGPKLYVPIFLAMPDILHALGLSDRPDAATLSLSRGKRRWTVTVPAGQV
                     DPSWPPDTDISLVTPDGWVDARAAPRPPLWLQAPLDYHRLVELPEHKALYAQLNMVTN
                     VEGQTLGDYGRKIRERVEATNPRAVILDLRLNRGGNGNLRNAFVPELIKAEDEDTRLF
                     VLTWRGTFSASQFILDDLDRLSGAIVIGEPASSRPTSYGDGYRIMLPNSGITARASIL
                     YWQAGQNDDPWTWVDIAAPLSFAAYAAARDPALEAALAYVPRPTLRDQVTAAAGAGGI
                     AAVLRAAEAWRADPAHRYSDTELQMILAAQGLGKAHPAEAIALAELGARHHPRSADAF
                     NVLAHLAEDAGRTELAREAGRRALALEPNDRAVRALLERLN"
     gene            complement(10552..>11240)
                     /locus_tag="VF589_02635"
     CDS             complement(10552..>11240)
                     /locus_tag="VF589_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8256506.1"
                     /translation="IAHVLDPARPRGAARLGWAAGSLAAAIALNGALAAAEPIVEAPF
                     GRQAGAGEAAAAKLARLDSPQARTIARAMRLRDWNARRVQGSTLFDEPAAARPLLAAL
                     RDDQPAVRRIALWGLSEMRPAPAGAAAPVARLLADPAAEVRGEAARALGDLGSVAHAE
                     DVAALLRDPHAAVRRQAAHALGDLQDPAIAPALEAALNDPDPSVRDKAAWALRQVREA
                     ETILRRYRSG"
BASE COUNT         1775 a         3677 c         3980 g         1808 t
ORIGIN      
        1 gtcgaatatc tcacgctcca gccagccggc gaccggccac agcccggtga ccgagggcac
       61 cggcttctcg tcgtcggtgt ggacgcgcag gcgaatgcgg tggttcttcg tcaccgagag
      121 caggtgatag accacttcga accgctcggc ccgctgcgga tagtcgacgc cggctatctc
      181 catcagctgc tgatattcga agcggtcgcg cagcgtgcgg cagacttcga ggatgctctc
      241 gcgcgcgacc gtgatcgaga cttcgccgac cgcgtccttg gcgtcgatga aggcatcgcc
      301 gatggcggcg cggacttctt cgatgatgcc ttcgcgcgga gagatgcggg gtgcggaaga
      361 gctcatgaca ccattccatc ccgttcgtgt cgagcccttc gacgccgccc gcggcggcgc
      421 tctggatgaa cttcgtcgag acaccccttc gagcgaagcc gagaaacaga ggcctcggct
      481 ccgctcggcg gggcccctcg acttcgctcg gggcgaacga attgaacagc gaacgccact
      541 ctatcgctcg atcgtgcctt ggcggcggat tttccgctgg agctgcatga tgccgtagag
      601 cagggcttcg gcggtcggcg ggcagccggg gacgtagatg tcgaccggca cgatccggtc
      661 gcagccgcgc accaccgaat agctgtaatg ataatagccg ccgccattgg cgcagctgcc
      721 catcgagatc acgtagcgcg gctccgacat ctggtcgtag acgcggcgca gcgccggggc
      781 catcttgttg caaagcgttc cggcgacgat catcacgtcg gactggcgcg gggacgcccg
      841 cggggcgacg ccgaaccgct cgaggtcgta gcgcggcatg ttgacgtgga tcatctcgac
      901 cgcgcagcag gcgaggccga aggtcatcca ccacaaggag ccggtgcggg cccattggaa
      961 caggtcctcg gtcgaggtga cgaggaaacc cttgtcggcg acctcggcct ggagctcgtt
     1021 gaaatattgg gggtccggct gctggagatc ggcggggcgg acattctcgt ggcggggatc
     1081 gaggatcact cccattcgag cgctcccttc ttccaggcat agacgaagcc caaggtgagc
     1141 tcggcgagga agatcatcat cgcggcccag ccggcgatgc cgatcgcgcc cagcgagacc
     1201 gcccagggga acaggaacgc ggcttcgagg tcgaagatga tgaacaggat cgcgaccaga
     1261 tagaagcgga cgtcgaattg cgagcggctg tcctcgaagg cggggaagcc gcattcatat
     1321 tccgcgagct tgtccggata gggattctgc gcgccggtga agcgggagac gatcatcggc
     1381 aggaacacga acgaagccga aagcgacagc gccacgccga ggaacagcag gatgggcaga
     1441 tattcggcgg cggtggacgc catcgagact ctccggggaa caacaggttg cggcggcttc
     1501 taggccagcg gacccgcctc cgcaaccgcc tcggcggctt gacagcggca ggcccggaat
     1561 ggaggatgtt ggcggggata tagggggagt gaaatggcaa tcaaccgagt gatcctgctc
     1621 gcctcggccg ccttgctggg cacgcctgcc ggggcccaga tggtcgcggc gaaggacccg
     1681 cagaccctgg tcgcggcgct gcaagcgaag ggttaccagg cggagctcgg cgagtcgggc
     1741 ggcgagccgc gcatcaagag cggcgccggc ggcgtcgcat tctcgatcta tttcgacaat
     1801 tgcacggcgg gcgcggcctg caagacgatc agcttcgtga ccggtttcac cgacgtcgac
     1861 gcgacccttg ccgcggtcaa cgagtggaac gcgaagaacc ggttcgcgca cgcctatatc
     1921 gatgccgagc aggacccgat gctgcggatg gacgtggatc tcgaccacca gggcatcccg
     1981 cgcgcgaatt tcggcgaata tctcgacatc tgggcatctt tggtgcccaa attcctgaac
     2041 tatcttcggc aggacgggaa ataaccgccg ggcgatacgg gtcccggtca ccctgaacgc
     2101 gttcagcctc gccagctccg ccgcccgcgg gcagcggcct cgccgtcgga agctccgcct
     2161 agccgcgcag tgcgcccgcg accagtttgt ggagccgggt ctggatctgg tcgttcgagg
     2221 cgagcacctg gccggtctcg agcgccttgt cggcgccgcg gaagtcggtg acgaagccgc
     2281 ccgcctcgcg caccagcagc acgccggcgg cgatgtccca atattcgaga tcctcttccc
     2341 agaagccgtc atagcggccg gcggcgaccc aggcgaggtc gagcgcggcc gagccgaagc
     2401 ggcggatgcc ggcgacttcg ggggcgacgg cggcgaggat cttctcgaac cgggcatggt
     2461 cgccatggcc cttatagggg atgccggtgg cgacgagcgc gtcggcgagg tcgcggcgcg
     2521 aggaaacgcg caagcggcgc tcattcagcc aggcgcccct gcccttttcg gcccagaagc
     2581 tttcgtcggt gagcggctga tagaccagcc cctgcgtgat ttcgggccgg ccgccggggc
     2641 cgcgcggatc ctcgaccgcg atcgagatcg cgaaatgcgg gatgccgtgg aggaaattgg
     2701 aggtgccgtc gatcgggtcg atgatccagc gcggcttgga cggatcgccg gcgacggtgc
     2761 cgccctcctc catgtggaag ccccagtccg ggcgcgcgtg gcggagctcc tcgaccaagg
     2821 tctgctcggc gcgcttgtcg gcgagcgaca cgaagtcggc cgggcccttg cggctgacct
     2881 gcagcagctc gacctcgccg aagtctcggc ggatgcgcgg cgcggccttg cgggcggcgc
     2941 gctgcatgac ggtaatgagg ccgctgtgac ttaccactcg agagctccgt tcgtgtcgag
     3001 cgaagtcgag acatcccttc gggcgaaggc gagaaatagg gcctcgactt cgctcggcaa
     3061 cgcccctcga cttcgctcgg ggcgaacggc tctattccgc tctctcaatc cgccctgcgg
     3121 acgtaggtct gttcgtaaac atcgactacg atccgggttc cgctgccgat atgcggcggg
     3181 accatgacgc ggacgccgtt ttcgaggatg gcgggcttgt agctcgacga ggcggtctgg
     3241 cccttcacga ccgcgtcggc ctcgacgatg gtggcttcga tcgtgtcggg gagctgcacc
     3301 gagatcgggc gctcctccca cagctccatc accacgtcca tgccgtcctg gaggaaggcg
     3361 gcggggtcgc cgatcaggtc gcgcggcagc gacacctgct cgtaattatc cttgtccatg
     3421 aagacgaggc tgtcgccgtc ggcgaacagg aactggaagt ccttggtgtc gagccggacg
     3481 cgctcgaccg tctcggccga gcggaagcgg acgttgttct tgcggccgtc gatgaggttc
     3541 ttcatctcga cctgcatata ggcgccgccc ttgcccggct gggtgtgctg gatcttgacg
     3601 gcgcgccaga tgccgccttc atattcgatg atgttgccgg gacggatgtc cacgccgctg
     3661 atcttcatgc aaatctcgct atcgggaaag agccgccggg gctttagcgg gcgggccgct
     3721 ttccggcaag cagcggatag gggttgaccg ccctgccctc gtgccagtcc tcgcccggcg
     3781 ccatgcggtg gatggcgaag tggaggtggg ggccgtcggg gttggaattg ccggtgacgc
     3841 cgacgcggcc gatcggctgg ccctggcgga tggcctgccc ttccttgagg ccgggcgcgt
     3901 aatcctggag gtgggcgtaa tagaaaacgt ggcgctggtc gggtgagcgt acgtagacgg
     3961 tgatgccgcc gccgcctttg ctgaagaaaa gcttctcgac ggttccgggc gccgcggcca
     4021 ccaccggcgt gccgtgatcg gccatgatgt cgatggcgtc gtgcgagcgg ctgccgccgg
     4081 cgcgggcctg ggtgtaggtg tcgaggaggt cggaggccct gatgccggcg acggggacgg
     4141 cgaggccgga cgggccaata tcgacgtcgc ccgcggcctc gccggcggcg gccctctttt
     4201 cggcggtctc ggcggccggc tccgtcttcc gccgcccgtc ctcatcgcca taagcggcga
     4261 tccagaaggc gctcgtcacc accgccacga cgatggcggt gacgaggttg ctgaggatga
     4321 gcttcagctt tcccatcccg acagcctaac cggatcaggc ctggaacact acctcgatgc
     4381 cgtcctcgat catctgcgtc aggcgagcgg cgtcccaatt ggtgaggcgc acgcagccat
     4441 ggctttcggc gcgcttgatg gtctcggggc tgttggtgcc gtggatgccg atatgctcct
     4501 tgttgagatc gatccagacc acgccgactg ggccgttcgg ccccggcggg aggagctgct
     4561 cgggctcgtc gtccttgacg tcccagaaga gctgcggctg atatttgaag ggcgggttga
     4621 aggcgacgct gttcaccttc caattgccca gcggcagcgg gtccttctcg ctgcccatcg
     4681 tcgccgggaa ttgcgcgatc gggcggccat tctcggcata gacgtggacg accttgtcgg
     4741 acttgtcgac gaccaccttg gccgccttgg gctggtcggc gctgacgtta aggtcgctga
     4801 ggagctgcgc cacgtccggc ttcacatcct gatagttgcg cgaggtcggc agcacgttgg
     4861 gcagccgcag cacggtgccg gcgcggagcg ccatgcgcgg gttgttgagg gcgatgatcg
     4921 tcgccggcgt ggtgtggaat ttctccgaga tcttctcgag caggttgcga taggcgagcc
     4981 gctccatctt cgcctgctgc tcgggctcgc tcgggatcgc ggtgaacggc ccccgcgcat
     5041 cgctctcgtc gatccgcagc cggcggaccg aaggcgcggg gtcgcgcagc aaggtggcgc
     5101 gggtcgaatt gtcgagctgg ccggtggcct ggaggccgcg cgcctgctga aagcccttca
     5161 gcgccgcttc gaaggaaatg ccggccatgc cgtcgatgac gccgggcgaa aagccggcgc
     5221 ggtcgagcag cacctgggcg tgaaacacgc tgccgtcgaa cttctcctgg gcggctgcgg
     5281 gcttttgctg gctggcggga tcgtcctgct gctgggcgac tgcggctccg gcgagggcga
     5341 cggcggcgct cccgaagagg aaggcgggaa gcaatttcga cactggggta cgctccactg
     5401 aattacggga ttcgcccctg acaacgccgc agccgaaact tatttccgtg ggccgccgtt
     5461 cgggcgggat gacaggcgcg atccggatcg gctgctcggg ctggaactac aagcattggc
     5521 gcgagctctt ctacccgaag gggctgccgc agcggctgtg gttcgaatat tattcgggca
     5581 ttttcgacac ggtcgagatc aacaacagct tctatatgct ccccaaggcc gagacgttcg
     5641 ccaagtggcg cgaccaggcg ccgccgggct tctgctatgc ggtcaaggcc aaccgcttcc
     5701 tcacccaggc caagaaactg aaggattgcg ccgagccgct cgagcggatg ctgacgccca
     5761 tgcgcaacct cggcgaccgg ctcgggccga tcctctacca gctgccgccc cgcttccggg
     5821 tgaacctgga gcggctggcc gaattcatcg acctgctgcc gaaggacctc acccacgtct
     5881 tcgagttccg cgagaagagc tggctgaccg aggaggtgct ggcgctgctc gacgcgcgcg
     5941 gcgtcgcctt ttgcgtccac gatatgcccg ggtccgcgac gccgcgctgg gcggcggggc
     6001 cgatcgccta tgtccgcttc cacggcggcg agggcaaata ttggggccgc tattcggacg
     6061 aggggctgct aagctggatc gactggatcg tcgagcagtc gcgcgccggc cgcccggtct
     6121 ggtgctattt caacaacgat atcgacgccc acgccatcca cgacgcgctc accttgcggg
     6181 cgatggtcgg gcaggcggcg cggtgaagcg gccgatccag gcctaagttg ctctggataa
     6241 atgggaatat tcgagagacg ggattgactc tgtgccaccc gtcactaagc gcttgtttcc
     6301 gaacgaatat tgcgttgcgc cacggcgctg aggggagttt gcatggggta tagcggcgtt
     6361 ttcgtgttcg gcgacagcct ggtcgatgca ggtaatgcgc tgaagctggc ggaattcgcc
     6421 gaggatatct ccctggccga actccccgcc ggcgtgccga ccagcgagaa gggctattat
     6481 gaggggcggt tcaccgacgg cctcaccttc gccgacctgg tcagcaacaa gttcatctcg
     6541 atgccgacca cgacggtgtt cccgttcggc ttcaccgatc cctggatcgg cgtctccttc
     6601 cggttcgtgt cggaccccga ggggaacaac ctcaacttcg cttatggcgg cgcgcagatc
     6661 cgcaagggcg aggaagtcgt gcccgacctc gacgaccaga ccgacgcctt catcgacgcc
     6721 gtcgacggcg atgccgatcc caatgcgctg catatggtca cgatgggcgg caacgacgtg
     6781 cgccggctgg tcccagacga cgcccccgtc gccgatgccg cgaccgcgcg cacgatcctc
     6841 gccgacgcgg cgcaggagat gcacgaagag gtcggcgacc tgattcgggc cggcgttcgc
     6901 cacgtcgtgg tgaccggcat tcccgacgtc ggcatgatcc ccgaatataa cggtctcgcc
     6961 gacgaagccg cccgccgcgc cgccgcgacc gaatattcgg ccattctcga cggcatgatc
     7021 caggattatc tcggccagct ccgcgtccaa tatcccgcgg ccaccatcca ttatgtgagc
     7081 ctgaccgatg cgacgacgaa gatcgtcgac aatctcgacc ggatcttcgc tccggaagat
     7141 ctcgcggggc tgcacaatcc cagcgacgtc ctgtttttcg atttcgtcca cccgaccgcc
     7201 caggcgcaca ccctgctcgc ctcggccgtg ctcgattcga tgagcggcgc gccggccggc
     7261 gaacgctacg ccctcaccgc gcccgactac cgcgtggccg ggaacatcgg ccaggtggga
     7321 gaggtcgaca cgatcaccct ctcgctcgcc gccaacaccc gctacacgtt cgaattgctc
     7381 ggcctcagct ccggctcggg ctcgctcgcc gatccccggc tccagctgct cgggccgggc
     7441 aatgtcgtgt tcggcggcga cgatgacggc ggcctcggcc tcgacgcctc gctgcagttc
     7501 accaccggcc aggcgggcga ctatgtcgtc cagatgggcg gcgtcggcag cctttccggc
     7561 ggctacagct tccaggcctc cgggccgttc cagatcgtcc acaacggcac ccaggccgac
     7621 gacaggctgg tcggcacgaa cgtcgccgac cggattcgcg gcctcggcgg caacgacctg
     7681 ctcgacggcg gcgccggcgc ggatgtcatg gaaggcggcg ccggcaacga cacctatctc
     7741 atcgacaatg ccggcgacca ggtggtggaa acctcccgcg gcgggaccga ccatgtgctc
     7801 gcctcggcca gccaccggat gggggcatgg gtcgagacgc tcaccctcgc ggacggcgcg
     7861 ctcagcggca ccggcaattc gatcgacaat gtcatcgtcg gcaacgcggc ggccaacagc
     7921 ctcaatggcg gccggggcgc cgaccgcctc gaaggcggag cgggcaacga ccgatatgcg
     7981 gtggaccagg ccggcgatgt cgtggtcgag gccgccgatg ggggccgcga cgtggtcagc
     8041 agcgcggtga gctacagcct cggcgacaat gtcgaagatt tggtgctgac cggtgccggc
     8101 cacacggccg gcaccggcaa tagcctcggc aaccggatga gcggcaatgg cggcggcaat
     8161 gtcctgaagg gcgaagccgg aggcgacacg ctccgcggga tggacggcgc cgacgacctg
     8221 tatggcggcg atgccaatga cgtgctcgag ggcggagcgg gtgcggaccg gttccatttc
     8281 gatgcggcgc tgaacgcgcg ctcgaacgtc gaccgcatcc tcgatttctc ctccgccgac
     8341 gacacgatca tgctcgatac cgcgatcttc ggcaacgtcc cggagagtgg cgcgctctcg
     8401 gaagccgcgt tccggcaggg caatgccgct cgcgatgccg acgaccggat cgtctacaac
     8461 gaggccaccg gcaatctcta ttacgatgcc gacggggcgg gcggcgccgc catggtcctg
     8521 ttcgcgcgcg tcgcggcggg cacgacgctc gaccatctcg atttcgccgc ggggctatag
     8581 ccgcggcgcg ggacgcttag gcgggaacca gggccggcag cgcggtgacg gcggcaaccg
     8641 tcgcgagcgc ggcaacgagc ccgagctcga ccgcggaaga tcgaatccgc gcgcggatgg
     8701 tgttgaaagc gttggtcata atatcctcgt ccaatccgga ggcgcctgcg gcgaattgcg
     8761 gcggcggcct cccacctgac ccatcgacgc gccacgcttt cgcccgttcc ggcaatccgc
     8821 ccaagcggtt gcactggcgg gacgaaccgc cggaatcggc ctaattcagc cgctccagca
     8881 gcgcccggac cgcgcggtcg ttgggctcca gcgcgagcgc tcgccgcccc gcctcccgcg
     8941 ccagctcggt ccgtccggca tcttcggcga ggtgggcgag cacgttgaag gcgtcggcgc
     9001 tccgaggatg atggcgggcg ccaagctcgg ccagcgcgat cgcttcggcg gggtgggctt
     9061 tgccgaggcc ctgcgccgcg aggatcatct gcagctcagt atcggaataa cggtgagcgg
     9121 ggtcggcgcg ccaggcttcg gcggcccgca gcacggcggc aatccctccg gcgccggcgg
     9181 cggcggtgac ctggtcgcga agcgtggggc gcggcacata agcgagcgcg gcctccagcg
     9241 ctgggtcgcg ggcggcggca taagcggcga agctcaatgg ggcggcgatg tcgacccagg
     9301 tccagggatc gtcattctgc cccgcctgcc agtagaggat ggaggcgcgc gcggtgatgc
     9361 cgctgttcgg gagcattatc cggtagccgt cgccatagga ggtcggccgc gacgaagcgg
     9421 gctcgccgat gacgatcgcg ccgctgagcc ggtcgagatc gtcgaggatg aactggctgg
     9481 ccgagaaggt gccgcgccag gtgagcacga acaggcgggt gtcctcgtcc tccgccttga
     9541 tcagctcggg cacgaaggcg ttgcgaagat tgccgttgcc gccgcggttg aggcggaggt
     9601 cgaggatcac cgcgcggggg ttggtcgcct cgacccgctc ccggattttg cggccatagt
     9661 cgccgagcgt ctggccctcg acgttggtga ccatgttgag ctgcgcgtag agcgccttat
     9721 gctcgggcag ctcgaccagg cggtgatagt cgagcggcgc ctgcagccac aaaggcggcc
     9781 gcggcgcggc gcgggcgtcg acccagccgt cgggcgtcac cagcgaaatg tcggtgtcgg
     9841 gcggccagct cggatcgacc tggccggcgg ggacggtgac ggtccagcgg cgcttgcccc
     9901 tgctcaggga gagcgtggcg gcgtcgggac ggtccgacag tccgagcgcg tggaggatgt
     9961 cgggcatggc gaggaagatc ggcacgtaga gcttggggcc gatggcgttc tcggcggcgc
    10021 tgatctcggc cgcgcggcgg atggcctccg ccaccggtac gccgccgatc gcctccacct
    10081 tggcgccgac cagcccggcc tgcgcgggcg cggcggcgcg gacgtagagg ccgtcctcga
    10141 acaggtaaag cttcagcggg agcgagggga aaccgaattt ggcatccttg agcggctcga
    10201 tccgggtgtg gccgtcgccg accatcgccg cgatccgcat caggcccacg atgatctggt
    10261 tgcggcggag cgtggggatg cgggcgtgga gatcggcgac cgcggcggcg aagcgctcgc
    10321 gactgacctc gtggtagagg ttcttgtgcc ggcgctccat ttcggcggcc atgaaggcga
    10381 ggtcggcgcg ccattgctcg gcggtgagat tggcaacccg ccccgcctgg gcgggcgccg
    10441 aagccgaagc ttgcgtcggc atggcggcgg ccagggcgag cgccgtcgcc ccgaggatca
    10501 gggttctcat cttggcagtt ccttgtggcg ggggttgagg ggcggcggcg ctcagccgct
    10561 gcgatatcgt cgcaggatcg tctcggcttc gcgcacctgg cgcagcgccc aggccgcctt
    10621 gtcgcgaacc gacgggtcgg gatcgttcaa cgccgcttcg agcgcgggtg cgatcgccgg
    10681 gtcctggagg tcgccgagcg cgtgcgccgc ctgccgcctc accgcggcgt gcgggtcgcg
    10741 gagcaaggcg gcgacgtcct ccgcatgggc gacggagccc aggtctccga gcgcacgcgc
    10801 cgcctcccca cggacctcgg cggcggggtc ggccagcagc cgcgccaccg gggcggcggc
    10861 gcccgccgga gccggccgca tctcggacag gccccacagc gcgatccgcc gcaccgccgg
    10921 ctgatcgtcg cgcagcgccg ccagcagcgg gcgcgccgcg gccggctcgt cgaacaaggt
    10981 gctgccctgg acccggcgcg cgttccagtc gcggaggcgc atcgcgcggg cgatggtccg
    11041 cgcctgcggg ctgtcgaggc gggcgagctt ggccgcggcg gcttcgcccg cgcccgcctg
    11101 ccggccgaac ggcgcctcga cgatcggctc cgccgcggcg agcgcgccat tgagagcgat
    11161 agcggccgcg agcgagcctg cggcccagcc gagccgcgcg gcgccgcgcg ggcgagcggg
    11221 atcgagcaca tgggcgatgc
//
LOCUS       DASVRK010000078        13499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_449142, whole genome shotgun sequence.
ACCESSION   DASVRK010000078 DASVRK010000000
VERSION     DASVRK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13499
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449142"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF589_02640"
     CDS             <1..243
                     /locus_tag="VF589_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256507.1"
                     /translation="GAGELAGYPVPAPMLEALRLAEPETAAALRTVRLASDAAEADHK
                     LPGPALWRRSEPGNSLELADAIALDIEIWVRQCDAS"
     gene            228..1022
                     /locus_tag="VF589_02645"
     CDS             228..1022
                     /locus_tag="VF589_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HEX8256508.1"
                     /translation="MRRLLSFRCDGAALAASLDAAEGPTGVLIVTGGSQTRIGSHRLF
                     ERLAQGLAAAGYPAFRFDRRGVGDSEGEDPGFRGSGPDLAAAAAAFRSQTPGVPRVVG
                     LGLCDGATAGALFGAAAGLDGLILVNPWLVEAEADAPPPAAIRRHYRERLTSLEGWKK
                     IATGAISWRKLLKGFSKIAKAAPSPLAADVAAALEAGKRPVALILARGDATAIAAEAE
                     WRSARFAAVREVSPPILHVETDSHTFARAGDEAALLTACLAALSRF"
     gene            complement(1008..1973)
                     /gene="trxB"
                     /locus_tag="VF589_02650"
     CDS             complement(1008..1973)
                     /gene="trxB"
                     /locus_tag="VF589_02650"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX8256509.1"
                     /translation="MAATHSTRMLILGSGPAGLSAAIYGARAGLAPIVVQGIQPGGQL
                     TTTTDVENYPGFRDVIQGPWLMEQMQAQAEHVGAQMMWDHIVEVDLSRRPFRLIGDGG
                     TIYSGDTLVIATGAQAKWLNLPSEEHLKGRGVSACATCDGFFYRGKKVAVIGGGNTAV
                     EEALYMTNHSHDVTLIHRRDSLRAEKILQERLFKHPNIKVLWNREVARFVAGGEPEGL
                     VALELRDTRTGEIERLETEGGFVAIGHTPATELFKDHLRLDADGYIEVETGTTLTSVP
                     GVFACGDVMDKVYRQAVTAAGTGCMAALDAEKFLAEMEFEASEAA"
     gene            2120..3193
                     /gene="hppD"
                     /locus_tag="VF589_02655"
     CDS             2120..3193
                     /gene="hppD"
                     /locus_tag="VF589_02655"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX8256510.1"
                     /translation="MTAHDNPLGLDGFEFVEFTGPDPEALAGLFTAMGFTHLGNHRSK
                     NVRHYGQGDINFILNMEPSGQAGSFRSAHGPSANAMAFRVKDAARAFEMAVERGATPV
                     EVARGPGELDIPAIEGIGGSYLYLVDRFGAEQIYDVDFEPVPGASRDGNAAGLHTLDH
                     LTHNVNRGRMDHWAQFYETIFNFRQIRYFDIEGQQTALVSRAMTAPDDKIRIPLNESQ
                     DEHSQIEEFLREYKGEGIQHIALSTDNIFGTVDRLRANGIRFQDSPETYFDLIDQRLP
                     GHGHDVAEMRKRRILIDGSPETGEGLLLQIFTENMIGPIFFEIIQRKGNQGFGEGNFK
                     ALFESIELDQIRRGVLPAAKEHA"
     gene            3219..3992
                     /locus_tag="VF589_02660"
     CDS             3219..3992
                     /locus_tag="VF589_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX8256511.1"
                     /translation="MRHISDSEAGGDLLQVREFVAAAQQAETLPDLHALLEAVCADLD
                     IDHYLFASHVDFGNPPPGAVALGNYPREWVARARQKGAWRSDPILAACEKTSAGFLWS
                     ELARLIPMSGPQYAFVREGQRFGLGDGFSVPNHVPGELLGSCHFAVRPGVAIADRNVS
                     MLQTVASFGFEAARVLSRDVERPVVLNAPLTDRQRDCVLLSAKGKSDSTIAQLLGLRP
                     RTVNEHMEGAKRRFCVATRQQLIVRALYASQITFSEVLQ"
     gene            complement(3967..5415)
                     /locus_tag="VF589_02665"
     CDS             complement(3967..5415)
                     /locus_tag="VF589_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8256512.1"
                     /translation="MATLAARLLPAAPISAAPVAKAAPAAARWTFERADVDALPKIRR
                     ADVAHIPGKLGLPFVGIVPEALVDPLKFAERMYERYGPVYRFYGRGNWNVQLIGAEAN
                     ELVLFDKAGSFSAYGGWAPLVEPFFPGALLVKDGQDHRTSRRIIGEAFRQDRLGGYQR
                     IFAADIDSAVSSWEQGPVDVYEEVKALTLRIAASTFLGLPLDQGGGAAVRHFSYMMGA
                     LLAVVRSPWLSPVAARGFWSKARLEAQMGGLIQQKRVSLDDDIFSRVCRLKDEEGRLL
                     SDREIVDSLIFLLAAAHDTLASGLTSVVVFLASEPGWADWLREELEGVRLDDPADAAT
                     AALPRQDMFFKEAIRLNAPAPIVWRRAVRDVEIYGHRIPAGTITGVNPLLTHRIAGLW
                     DRPTEFDPLRFAPEAEAARHKFAFVPFGGGIHKCLGLHFSMQQARIFMTRLLRCESVE
                     IDRGKGVSWYNWPNCRPRGRVMGRFTAAPPKR"
     gene            5543..6172
                     /locus_tag="VF589_02670"
     CDS             5543..6172
                     /locus_tag="VF589_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="HEX8256513.1"
                     /translation="MIDVVDPASRWRFSQPLMEMYHHRKRVFVDQLGWALPSAGSWLE
                     LDEFDHDHAVYLLARDRGEGTHLGSVRLLPTTRPHMLTTTFAELCPDGPPQGEDCWEI
                     SRLVTNPATVRGSTCIRVHRMLAGALVDFARLNGVRRYTLVAEISRVPALLAVGWLVT
                     PLSLARRVGAETIQALQIDVDEATGERLRRRSGSDRPVLAGALSGRCAA"
     gene            6229..6762
                     /locus_tag="VF589_02675"
     CDS             6229..6762
                     /locus_tag="VF589_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256514.1"
                     /translation="MTSPPERPSDADEPALPLPLSTERIESFARRVGMLADLLPRART
                     ASPPGEIAAFARQLYEERRIRDRIFAGDLFGEPAWDIMLDLYAAEEEGRETSLAGACA
                     AAAVPPSTGLRYLNAMLELGLIVSEGGSEAGEDGRVRLSGPSRAQMRQLLGRMIAARH
                     SHGGADEAQDRDEPPPG"
     gene            6725..6934
                     /locus_tag="VF589_02680"
     CDS             6725..6934
                     /locus_tag="VF589_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256515.1"
                     /translation="MKRKIEMSRRLAEPQSIERLEAGAQAPLDVEQRIIAMLSGGGGV
                     PSIFTGHHGISSPTARAGDQAAYNA"
     gene            complement(7032..7400)
                     /locus_tag="VF589_02685"
     CDS             complement(7032..7400)
                     /locus_tag="VF589_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8256516.1"
                     /translation="MSFGNRLAEERKRLGLKQVEFAGRVNTDVAKQSLYENDRRDLRA
                     DYLARLAEAGVDVLYVLTGRRSEGHWLGSGASDLLSAYLGLPPDMQQALLTLAQTLSG
                     QFAREGSRTVHSKNLDYRAD"
     gene            7512..7685
                     /locus_tag="VF589_02690"
     CDS             7512..7685
                     /locus_tag="VF589_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256517.1"
                     /translation="MRLDEANPDERVLAAAASAVLAALRGPLDQEQLPGPAVAALRLL
                     ETALLPYDYRREQ"
     gene            7738..8991
                     /locus_tag="VF589_02695"
     CDS             7738..8991
                     /locus_tag="VF589_02695"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8256518.1"
                     /translation="MPNPTLDWRHKALPEAASGLDGGAIGALGLRLLDGDLMMPAAIL
                     RDAALRHNILAMQAFADAHGARLCPHGKTSMSPELFAMQAEAGAWGLTAATAHHVRIY
                     RRLGISRILLANQLVGRADIAFVLDEIARDPEFDFYCLVDSPEAVELLAAAARAAACP
                     RPVQLLVEAGIGGGRTGLRDPAEALALARKVAAAAPLLALRGIETYEGIHQARPGAAA
                     EAADMLRRAAELTARALAERLFAPGPVILSAGGSAFLDLCTAALPARIAGTPIERVLR
                     PGCYVTHDSGLYVRLTDGSEARGLPELRHALEVWGLVQSVPEPGLAVVGLGKRDASFD
                     VEPPIPLWLHRPGRAGPPAPADGLRVLAMWDQHLSLASPAGALAVGDMVGFGISHPCA
                     TFDRWRALLRVDEEYRVTGAVTTLF"
     gene            complement(9083..10357)
                     /locus_tag="VF589_02700"
     CDS             complement(9083..10357)
                     /locus_tag="VF589_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX8256519.1"
                     /translation="MDPRVPRGMSGGFTVRRLAWSDPELGATPSAGKPIPIVAGFGSG
                     LSRRRGDPPGIVWAVGDRGPNFKVETAVERFGLSHFQILDVGAGAKIMPRLDLGPALA
                     ELRVLRDRVELVRSMPIADPRGRPISGLPVPRGAHAECEPALDLDGRPIPPDPGGADS
                     EGIAALADGGFWVGDEYGPSLLRLGPDGRILWRWVPAGQEPLFEGAGYPVEPALPAIA
                     AARRLNRGFEAIALSADEQWLYLAFQSPLAHPDEQAHAEAAHVRLWRLDAGSGAVTAQ
                     YLYPLDSPDSFRRDSAKGPLEKSDLKVSEIILDGADALLVLERGSETTKIYRIALDPA
                     AVIPDEHLDIDTRPTLEELSAGGDPGLPVLAKTLIFSSDDAPEVAADLEGMAILSRTE
                     LLLVNDNDFGVEGAETSFWVVSFDRPMFEAPA"
     gene            complement(10330..11013)
                     /locus_tag="VF589_02705"
     CDS             complement(10330..11013)
                     /locus_tag="VF589_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX8256520.1"
                     /translation="MKIGILETGKPPLDLAGRFGGYPEMFAKLLGEDYRYAVYDAQAG
                     ELPARPDEQAAYLITGSPAGVYDKLPWIAGLAAFLREAKGRAKLVGICFGHQIMAETF
                     GGRVVRSELGWAAGLQDYHVVRPAAWMDGAGTVAIPVSHQDQVIEQPPGSRVIGHSDF
                     SCFGMLEYEDQPAISFQCHPEFEPAYAKALIEVRRDRLPDPDGAIASLDAANDRARMG
                     GWIRGFLAA"
     gene            11406..11548
                     /locus_tag="VF589_02710"
                     /pseudo
     CDS             11406..11548
                     /locus_tag="VF589_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            12029..>13499
                     /locus_tag="VF589_02715"
     CDS             12029..>13499
                     /locus_tag="VF589_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8256521.1"
                     /translation="MAINHWADMMGIDASQMAALSAGEEPTAAMGDEPLATGPTDTIP
                     DEITNNNPELTVGGSPIISTIDAPGDQDVFKVELTAGQNYEFGQYATTDGPTHVPLTD
                     AYLEIYDAQGNLLATADGGGPDTPSGLDALLTFRPTESGTYYINARAFDNAPEDGTNG
                     DIVGDYEIFARESNYNAYYDVDSPLHSLDWGTQVDGTVRNPDGEEGPRPTGNEANGTP
                     ESPYQIEGKNVITYYFAETGDVYVTEDPTDPGLTDTIVASGMEDWEKQAFVDGFNSFS
                     AVADVVYVEVFNRAEADFNLITYNGTPQAGVLGRMSPPDTHNEGQAEFNRNGPGWNEE
                     QLRPGGFMFGTVVHELGHGHGMAHPHDNGGRSSVMRGVESTGGAFSYTLGAYNLNQGV
                     FTMMSYQDGWQSSPYGNSPDDGYGYLAGPMALDIAVLQDKYGVNEETATGDDIYRLKD
                     VNAAGTSYEAIWDAGGHDLVAYDGARDSKIDLRDATLQYE"
BASE COUNT         2180 a         4749 c         4507 g         2063 t
ORIGIN      
        1 ggcgccggcg agctcgccgg ctatccggtg ccggcgccaa tgctcgaggc gctgaggctg
       61 gcggagccgg agacggcggc ggcgctgcgc acggtgcggc tggcgagcga cgccgccgag
      121 gccgatcaca agcttcccgg tcccgccctc tggcgccgct ccgaacctgg caattcgtta
      181 gaacttgccg atgcaatcgc tttggatatt gagatttggg tgcggcaatg cgacgcctcc
      241 tgagcttccg atgcgacggc gccgcgctcg ccgcctcgct cgacgcggcg gagggaccga
      301 ccggcgtcct gatcgtcacc ggtggctcgc agacccggat cgggtcgcac cgcctgttcg
      361 agcggctggc ccaaggcctg gccgccgccg gctaccccgc cttccggttt gatcggcgcg
      421 gcgtcggcga cagcgagggg gaggatccgg gctttcgggg cagcggcccc gacctcgccg
      481 ccgcggcggc cgcgttccgg tcccagacgc cgggcgtccc gcgagtcgtc ggcctcgggc
      541 tgtgcgacgg cgccaccgcc ggcgctctct tcggagcggc ggcgggcctc gacggcctga
      601 tcctggtcaa cccctggttg gtcgaggccg aagccgatgc gccgccgccc gccgccatcc
      661 ggcgccatta tcgcgagcgc ctcaccagtc tcgaaggctg gaagaaaata gccacgggcg
      721 caatatcctg gcggaagctg ctcaaagggt tttcgaagat cgccaaggcc gcgccctccc
      781 cgcttgccgc cgacgtcgcc gccgcgctcg aagccgggaa gcggccggtc gccctcatcc
      841 tggcacgcgg cgacgccacc gccatcgccg ccgaagccga gtggcgctcc gcccgcttcg
      901 ccgccgtcag ggaagtctcg ccgccgatcc ttcatgtcga aaccgactcg cacaccttcg
      961 cccgagccgg cgacgaggcg gcgctgctga cggcgtgcct cgcggcgtta agccgcttct
     1021 gaggcttcga actccatttc cgcgaggaat ttctcggcgt cgagcgcggc catgcagccg
     1081 gtgccggcgg cggtcaccgc ctgccggtac actttgtcca tcacgtcgcc gcaggcgaac
     1141 acgcccggga cgctggtgag ggtggtgccg gtctcgacct cgatatatcc gtccgcgtcg
     1201 agccggaggt ggtccttgaa caattcggtc gccggcgtgt ggccgatcgc gacgaagccg
     1261 ccctcggtct cgagccgctc gatctcgccg gtgcgggtgt cgcgaagctc gagcgcgacc
     1321 aggccctccg gctcgccgcc ggcgacgaat cgcgccactt cgcggttcca cagcaccttg
     1381 atgttgggat gcttgaacag ccgctcctgg aggatcttct cggcgcgcag cgaatcacgg
     1441 cggtggatca aagtgacgtc atgactgtgg ttggtcatgt acagcgcctc ctcgaccgcg
     1501 gtgttgccgc cgccgatcac ggccaccttc ttgccgcggt agaaaaagcc gtcgcaggtc
     1561 gcgcaggccg agacccccct gcccttcaga tgttcttcgg acggtaggtt cagccatttg
     1621 gcctgggcgc cggtggcgat gaccaaagtg tcgcccgaat agatggtgcc gccgtcgccg
     1681 atcaggcgga acggccgccg gctgaggtcg acctcgacga tatggtccca catcatctgc
     1741 gccccgacat gttcggcctg ggcctgcatc tgctccatca gccaagggcc ctggatgacg
     1801 tcgcggaagc cgggataatt ttcgacgtcg gtggtggtgg tgagctggcc gccgggctgg
     1861 atgccctgga cgacgatcgg cgcgaggccg gcgcgggcgc cgtagatcgc ggccgacagg
     1921 cccgccgggc ccgacccgag gatcagcatg cgggtggaat gggtagcggc catgcgtgtc
     1981 tcgcttgagc ttaaactggg ttatgccggc gcgagatagg ggttcgcggg ctccgcgcca
     2041 acctttcccg catttgccgg gcggcggccg cgctgataat gctggaagcc aggaacggat
     2101 acgcgaaaag ggcctcgaca tgaccgcgca cgacaatccc ctcggcctcg acggtttcga
     2161 gttcgtcgaa ttcaccggac ccgatccgga ggcgctggcc ggattgttca ccgccatggg
     2221 gttcacgcat ctcggcaacc accgctccaa gaacgtgcgc cattacgggc agggcgacat
     2281 caacttcatc ctcaacatgg agccttcggg ccaggccggc agcttccgca gtgcccatgg
     2341 cccgtccgcc aacgcgatgg cattccgtgt gaaagacgca gcgagggcgt ttgaaatggc
     2401 ggtcgaaaga ggcgcgaccc cggtcgaggt ggcgcgcggg ccgggcgagc tcgacatccc
     2461 ggccatcgaa ggcattggcg gctcctatct ctatctggtc gaccgcttcg gcgccgagca
     2521 gatttacgac gtcgacttcg aaccggtgcc cggcgcgagc cgcgacggca atgcggccgg
     2581 cctccacacg ctcgaccacc tcacccacaa tgtgaaccgc ggccgcatgg atcactgggc
     2641 ccagttctac gagacgatct tcaacttccg gcagattcgc tatttcgaca tcgaggggca
     2701 gcagacggcg ctggtttcac gcgccatgac agcacccgac gacaagatcc ggattccgtt
     2761 gaacgaaagc caggacgagc acagccagat tgaggagttc ctgcgcgaat ataaaggcga
     2821 aggcatccag catatcgcgc tctccaccga caacattttc ggcacagtgg accggttgcg
     2881 cgccaacggt atccgcttcc aggacagccc cgaaacctat ttcgacctca tcgaccagcg
     2941 cctgcccggg cacggccacg acgtcgccga gatgcgcaag cgccggatcc tgatcgacgg
     3001 ctcgcccgag accggcgaag gcctgctgct tcagatcttc accgagaaca tgatcggacc
     3061 gatcttcttc gagatcatcc agcgcaaggg aaatcaaggc tttggcgagg gcaatttcaa
     3121 ggctctcttc gaatcgatcg aactcgacca gatccgccgc ggcgtgttgc cggcggccaa
     3181 ggaacatgcc tgagggcagc cgattgaagg gaccagcaat gcggcatatc agtgacagcg
     3241 aggcgggtgg ggacctgctc caggtcaggg agttcgtcgc cgccgcgcag caggcggaga
     3301 cgctgccgga tctgcacgct ctgctcgagg cggtctgcgc cgacctcgac atcgaccatt
     3361 atctgttcgc cagccatgtc gatttcggca acccgccgcc cggcgcggtg gcgctcggta
     3421 attacccgcg ggaatgggtc gcccgcgccc ggcagaaggg ggcgtggcgg agcgacccga
     3481 tcctcgccgc ctgcgagaag accagcgccg gcttcctctg gagcgagctc gcccggctga
     3541 tcccgatgtc agggccgcaa tatgccttcg ttcgggaggg ccagcggttc ggcctcggcg
     3601 acggcttcag cgtgcccaac catgtgccgg gcgagttgct cggatcgtgc catttcgcgg
     3661 tccgccccgg cgttgccatc gccgatcgca acgtctcgat gctacagacg gtcgccagct
     3721 tcggcttcga agcggcgcgc gtgctctccc gcgacgtcga acggccggtg gtgctgaacg
     3781 cgccgctcac cgaccgccag cgcgactgcg tcttgctctc ggccaagggc aagagcgact
     3841 cgaccatcgc ccaattgctg ggcctgcgcc cgcgcaccgt caacgagcat atggagggag
     3901 ccaagcgccg cttctgcgtc gccacccggc agcagctgat cgtgcgcgcg ctttacgcct
     3961 cgcagatcac cttttcggag gtgctgcagt aaagcggccc atcacccggc cgcgcgggcg
     4021 gcaattcggc caattgtacc acgacacccc cttcccgcgg tcgatctcga ccgattcgca
     4081 gcgcagcagc cgggtcatga agatgcgcgc ctgctgcatc gagaagtgga ggccgaggca
     4141 tttgtggatg cccccgccga acggcacgaa cgcgaatttg tggcgggcgg cctccgcctc
     4201 gggcgcgaag cggagcgggt cgaactcggt cggccggtcc cacaggccgg cgatgcggtg
     4261 ggtgagcagc gggttgacgc cggtgatcgt gccggccgga atgcggtggc cgtaaatctc
     4321 gacgtcccgg acggcgcggc gccagacgat cggcgccggc gcgttgagcc ggatcgcttc
     4381 cttgaagaac atgtcctggc gcggcagcgc ggcggtcgcg gcgtcggccg ggtcgtcgag
     4441 ccggaccccc tccagctcct cgcgcagcca gtccgcccag ccgggctcgg aggcgaggaa
     4501 cacgaccacc gaggtcaggc ccgaggccag agtgtcgtgc gcggccgcga gcaggaaaat
     4561 cagcgaatcg acgatctcgc ggtcggagag cagccggccc tcctcgtcct tcagccggca
     4621 gacgcgcgag aagatgtcgt cgtcgagcga gacgcgcttc tgctggatga ggccgcccat
     4681 ctgcgcctcg agcctggcct tgctccagaa gccgcgcgcc gccaccgggc tcagccaggg
     4741 cgagcgcacc accgccagga gggcgcccat catgtaggag aagtgccgga ccgcggcgcc
     4801 gccgccctgg tcgagcggca ggccgaggaa ggtcgaggcg gcgatccgca gcgtcagcgc
     4861 cttcacctcc tcatagacgt cgaccggacc ctgctcccat gacgagacgg cgctgtcgat
     4921 atcggccgcg aagatgcgct gatagccgcc cagccgatcc tgccggaacg cctcgccgat
     4981 gatccgccgg ctggtgcggt ggtcctgccc gtccttgacc agcaaggcgc cggggaaaaa
     5041 cggttcgacc aggggggccc agccgccata ggccgaaaac gagccggcct tgtcgaacag
     5101 gacgagctcg ttcgcttccg ccccgatcag ctgcacgttc caattgccgc ggccgtagaa
     5161 gcgatagacc ggcccgtagc gctcatacat gcgctcggcg aatttgagcg ggtcgaccag
     5221 cgcctcgggg acgatgccga cgaagggaag gccgagcttg cccggtatgt gcgccacgtc
     5281 cgcccgccgg atctttggca aggcgtcgac gtcggcacgc tcgaacgtcc accgcgccgc
     5341 cgccggcgcg gccttggcta cgggagctgc ggatattggg gcggccggca agagtcgggc
     5401 agcgagcgtg gccaaatgcg cctccttcgt tgggcaagga ggataggcac ggcgcgagcg
     5461 gcccggcgac cccggtgatt accggggtag cccgccgccg ccgctgcggc caccttgcct
     5521 cccgtcacag gaggaatgct gcatgatcga tgtcgtcgat cccgcctcgc gctggcggtt
     5581 ctcgcagccg ctgatggaga tgtaccatca ccgcaagcgc gtgttcgtcg atcagctcgg
     5641 ctgggcgctg ccgtcggccg gcagctggct ggagctggac gagttcgacc acgaccatgc
     5701 cgtctacctg ctcgcccgcg accgcggcga aggcacccat ctcgggtcgg tgcggctgct
     5761 gccgaccacc cgcccccaca tgctcaccac caccttcgcc gagctttgcc cggacgggcc
     5821 gccgcagggc gaggattgct gggagatatc gcggctcgtc accaatccgg cgacggtgcg
     5881 cggctcgacc tgcatcaggg tgcatcggat gctggccggg gcgctggtcg actttgcccg
     5941 cctgaacggg gtccgccgct acactttggt ggcggaaata tcgcgcgtcc cggctttgct
     6001 cgccgtgggc tggctggtca ccccgctgtc gctggccagg cgggtcggcg cggaaaccat
     6061 ccaggcgctg cagatcgacg tcgacgaggc caccggcgag cggctgaggc ggcggtccgg
     6121 ctcggaccgg ccggtgctcg ccggcgcctt gagcgggcgc tgcgccgcat gaaggagggc
     6181 cgtccgataa agcgcccccg cgcaatgcag ggagacggag accgacttat gacgtcaccg
     6241 cccgagcggc cgtccgatgc ggacgagccg gcgctgccgc tgccgctgtc gaccgagcgc
     6301 atcgaatcgt tcgcccgccg cgtcggcatg ctcgccgacc tgctgccgcg ggcgcgcacg
     6361 gccagcccgc ccggcgagat cgccgccttc gcgcgccagc tctacgagga gcgccggatc
     6421 cgcgaccgca tcttcgcggg cgacctgttc ggcgagcccg cctgggacat catgctagac
     6481 ctctatgcgg ccgaggagga aggccgcgag accagcctcg ccggcgcctg cgccgccgcg
     6541 gcggtgccgc cgagcacggg gcttcgctat ctcaacgcga tgctggagct cgggctgatc
     6601 gtctccgagg gcgggagcga ggccggcgaa gacgggcgcg tccggctcag cggcccgagc
     6661 cgcgcccaga tgcggcagct tctcggtcgg atgatcgcag ctcgtcacag tcacggcgga
     6721 gctgatgaag cgcaagatcg agatgagccg ccgcctggct gagccccaat ccatcgagcg
     6781 tctcgaggca ggcgcgcagg ctccgctcga cgtcgagcag cgcatcatag ccatgctgtc
     6841 cggcggcggc ggggtgccca gcatcttcac cggccatcac ggtatctcct caccaaccgc
     6901 tcgcgccggg gatcaagctg cctacaatgc ataaaagcgg atcgacagaa agcgcaaatc
     6961 cgtccgcttc ggcgcgggcg gggcggcgcc gagcggccgc ggcacgatcg cgcggtcgca
     7021 gccggcggaa ctcaatctgc ccggtagtcg aggttcttgc tatggacggt gcggctgccc
     7081 tcgcgggcga actggccgct gagcgtctgc gccagcgtca gcagcgcctg ctgcatgtcg
     7141 ggtggaagcc ccagataagc ggacaagagg tcgctcgccc cgctgcccag ccaatggccc
     7201 tcgctacggc ggccggtgag cacataaagc acgtcgactc ccgcctccgc caggcgcgcg
     7261 agataatcgg cacgcaagtc acgccggtcg ttctcgtaca ggctctgctt tgcgacgtcc
     7321 gtattgaccc ggcccgcaaa ttccacctgc ttcagtccca gccgtttgcg ctcttcagcc
     7381 agtctattgc cgaaactcat cgcttttgat tgttttccct tgactggtca acgggaatca
     7441 ttgactcaag cacccacctt tcgatgcatt gcacatgcac tcccgagatg tcgcgtggaa
     7501 atcaaaaacc tatgagactt gacgaggcga atccggacga aagagtgctg gccgccgccg
     7561 ccagcgccgt gctcgcggcg ctgcgtgggc cgctcgatca ggagcagctt cccggcccgg
     7621 ccgtcgcggc gctccggctg ctggagacgg cgcttcttcc ctacgactat cgccgcgagc
     7681 aataacggcc gccgccccgg cttgtgcacc ggggcccggc tcgattaggt ttgggcggtg
     7741 ccgaacccga cgctcgactg gcgacacaag gccctccccg aggcggcttc gggcctggac
     7801 ggcggcgcga tcggcgcgct cgggctgagg ctgctcgacg gcgacctgat gatgccggcg
     7861 gcgatcctcc gcgatgccgc gctgcgccac aatatcctcg ccatgcaggc cttcgccgat
     7921 gcccatggag ccaggctctg cccgcacggc aaaaccagca tgagccccga gctgttcgcg
     7981 atgcaggccg aggccggcgc ctggggcctg accgcggcga cggcgcatca tgtccgcatc
     8041 taccggcggc tgggcatcag ccgcatcctg ctcgcaaacc agctggtcgg ccgcgccgac
     8101 atcgcgttcg tgctcgatga aatcgcgcgc gaccccgaat tcgatttcta ctgccttgtc
     8161 gattcgcccg aagcggtgga gctgctcgca gcggcggcgc gggcggccgc atgcccgcgg
     8221 ccggtgcaac tgctggtcga ggccggcatc ggcggcggcc gcaccggcct tcgcgacccg
     8281 gccgaagcgc tcgccctcgc ccgcaaggtg gcggccgccg cgccgctgct cgcgctgcgc
     8341 gggatcgaaa cttatgaagg catccatcag gcccggcccg gtgccgccgc cgaagcggcc
     8401 gacatgctcc gccgcgcggc cgagctcacc gcgcgcgcgc tggcggagcg gctgttcgcg
     8461 ccgggaccgg tgatcctgtc ggccggcggc tccgcctttc tggacctgtg caccgccgct
     8521 ctccccgccc gcatcgccgg cacgccgatc gagcgggtgc tgcggccggg ctgctacgtc
     8581 acccacgaca gcggcctcta tgtgcgcctg accgacggca gcgaagcccg cgggctgccg
     8641 gagctgcgcc atgcgctcga agtctggggg ctcgtccagt cggtgcccga gcccgggctt
     8701 gccgtcgtcg ggctcggcaa gcgcgacgcc tcgttcgacg tcgaaccgcc cattcccttg
     8761 tggctgcacc gccccgggcg ggccggtccg ccggccccgg ccgatggcct gcgcgtgctc
     8821 gctatgtggg accagcatct cagcctcgcc tcgccggccg gagcgctcgc cgtcggcgac
     8881 atggtcggct tcggcatctc gcacccttgc gcgaccttcg accgctggcg ggcgctgctc
     8941 cgcgtcgacg aggaatatcg cgtcaccggc gccgtcacca ccttgttctg aggcgggtgc
     9001 tggaacgttc cccggcgaaa gccggggtcc agcggcgccg gcgcacacac taggctccgg
     9061 cctcccccgg ggtgcaatgg gcctacgcgg gcgcctcgaa catgggccga tcgaagctca
     9121 ccacccaaaa gctcgtctcc gccccctcga cgccgaaatc gttgtcgttg accagcagaa
     9181 gctcggtccg tgagaggatc gccatgcctt cgaggtcggc ggccacctcg ggcgcgtcgt
     9241 cgctgctgaa gatcagcgtc ttggcaagca ccggcaggcc gggatcgccg cccgcgctga
     9301 gctcctccag cgtggggcgg gtgtcgatat cgagatgctc gtcggggatc accgccgccg
     9361 gatcgagcgc gatccggtag atcttggtcg tctccgatcc gcgctccagc acgagcaaag
     9421 cgtcggcgcc gtcgaggatg atctcgctga ccttgaggtc gctcttctcg agcggcccct
     9481 tggcgctgtc gcggcggaag ctgtccggcg aatcgagcgg gtagagatat tgcgccgtaa
     9541 ccgcgcccga acccgcgtcg agccgccaca gccggacatg cgccgcctcg gcatgcgcct
     9601 gctcgtcggg gtgcgccagc gggctctgga aggcgaggta cagccattgc tcgtccgccg
     9661 acagggcgat cgcctcgaag ccgcggttga gccggcgcgc cgcggcgatc gccgggagcg
     9721 cgggttcgac cggatagccg gcgccttcga acagcggctc ctggccggcg ggaacccagc
     9781 gccaaaggat gcgcccgtcc ggccccagcc gcagcagcga cggcccatat tcgtcgccga
     9841 cccagaagcc gccatcggcg agcgcggcga tcccctcgct gtcggcgccg ccggggtcgg
     9901 gcgggatcgg ccgcccgtcg aggtcgagcg ccggctcgca ttcggcatgg gcgccgcgcg
     9961 gcaccggcag ccccgatatc ggccgccccc gcgggtcggc gatcggcatc gagcggacca
    10021 gctcgacccg atcgcggagc acgcgaagct cggccagcgc cgggccgagg tcgagccggg
    10081 gcatgatctt ggcgccggct ccgacgtcca ggatttgaaa gtggctgagc ccgaaccgct
    10141 ccaccgcggt ctcgaccttg aaattggggc cgcggtcgcc gaccgcccag actatccccg
    10201 gcggatcgcc gcggcggcgc gacaggcccg agccgaagcc ggcgacgatc gggatcggct
    10261 tgccggcgct cggggtcgcg cccagctccg ggtcggacca ggcgagcctg cggacggtga
    10321 agccgccgct catgccgcga ggaacccgcg gatccacccg cccatccgcg cccggtcgtt
    10381 ggcggcgtcg agcgaggcga tcgccccgtc gggatcgggc agccggtcgc gccgcacctc
    10441 gatcagcgcc ttggcgtaag cgggctcgaa ctcgggatgg cattggaagg agatggccgg
    10501 ctggtcctca tattcgagca tgccgaagca gctaaagtcg ctatggccga tgacgcggct
    10561 gcccggcggc tgctcgatta cctggtcctg gtgggagacc gggatcgcga ccgtgccggc
    10621 gccgtccatc caggcggcgg gccggacgac gtgataatcc tggagccccg ccgcccagcc
    10681 gagctccgac cgcaccaccc ggccgccgaa cgtctccgcc atgatctggt ggccgaagca
    10741 gatgccgacc agcttggccc tgcccttggc ttcgcgcaga aaggcggcca gcccggcgat
    10801 ccaggggagc ttgtcgtaga ccccggccgg cgagccggtg atcagatagg cggcctgctc
    10861 gtcgggccgc gctggcaact cgccggcttg cgcatcgtag acggcgtagc gataatcctc
    10921 gcccagcagc ttggcgaaca tctccggata gccaccgaac cgcccggcga ggtccagcgg
    10981 cggcttgccg gtctcgagga tgccgatctt catatgctta cgcccgcgta ccttggtcgc
    11041 gtcctaagac ccgtttccac gccgggattc aagcagcggc gcaacggcgc catctcccga
    11101 cccgcccgac ccgcgccggc ggaaggcggt ctccaggaag cgcgcccccg cgagctgcag
    11161 atgagcggct ctgaaagctc agcaccatcg cgcagatgaa cccggactgg cgcgacctct
    11221 cgtctgacag gctgcgcctg gtctgggccc ctgccttcgc aggggcacaa aatcgccaca
    11281 gaagccgcga ccattcgccg gcagccgcac ctgttcccct gcgaaggcag gggtccagtc
    11341 tcgggctggc gctttagcgg cagtccggtc atccctcccg gaggaaggcc ggatatgttt
    11401 acatcatggc gagcggccgc aacggcactc tatacgcgag gtgaccagcg atctcgcgca
    11461 gcgggtgtgg cagcatcgca gccatgtggt gcccggcttc acacgcgcat atggctgcaa
    11521 gctgcttgtc tggtatgaag ttcacgacga cacggcattc tgtttcccgc gcgttccaat
    11581 cggggaattc cgtaaggtga atcggggtcc gcggacgacg gaccgtgcag cccgagcagg
    11641 aacttagttg aagcttggag ggttttcact gggcatgccg gcgcttcggt tcggcatctc
    11701 aaggggcaat cggttggtat cgcacaaccg cggagacact gccggaaaca aggagcgttg
    11761 gccgcgggcc ggccgctccc ggccagtcga ccgcgcggca gcgacgccgc cccatcgctc
    11821 ccactcctcg cgtaatcgca gcatgccgga cgctcggtcc ggccggtggc taagcccccg
    11881 gccgatctcc cgcgcaccat gaacgggcgc gcacctcgct ggttcagcgg cacccgttcc
    11941 ccggcagaac gccagtctta ttcgataatg tcaaagacac aggagagata agatgtgctg
    12001 gacttgcaac ccgacgttga agagcgcctt ggccatcaat cattgggccg acatgatggg
    12061 gatcgacgct tcgcagatgg cggcgctgtc cgccggcgag gagcccaccg ccgcgatggg
    12121 cgatgagccg ctcgccaccg gcccgaccga cacgatcccg gacgaaatca ccaacaacaa
    12181 tcccgagctc acagtgggcg gaagcccgat catctccacc atcgacgcgc cgggcgacca
    12241 ggacgtgttc aaggtcgagc tgaccgccgg ccagaattat gaattcggcc aatatgcgac
    12301 caccgacggc ccgacccacg tgccgctgac cgacgcctat ctcgaaatct atgacgccca
    12361 gggcaacctg ctcgccaccg ccgacggcgg cggccccgac accccgtcgg ggctggatgc
    12421 gctgctcacc ttccgcccga ccgagagcgg cacttactat atcaacgcgc gcgccttcga
    12481 caacgcgccg gaagacggca ccaacggcga catcgtcggc gattatgaaa tattcgcccg
    12541 cgaatccaat tataacgctt attacgacgt cgacagcccg ctccactcgc tcgactgggg
    12601 cacccaggtc gacggcacgg tgcgcaatcc cgatggcgag gaaggtcccc ggccgaccgg
    12661 caacgaggcc aacggcacgc ccgaaagccc gtaccagatc gagggcaaga acgtcatcac
    12721 ttactatttc gccgagacgg gcgacgtcta tgtgaccgag gatccgaccg atcccggcct
    12781 caccgacacc atcgtcgcct cgggcatgga ggattgggag aagcaggcgt tcgtggacgg
    12841 cttcaactcg ttctcggccg tcgccgacgt ggtctatgtc gaggtgttca accgcgcgga
    12901 agcggacttc aacctcatca cctataacgg cacgccccag gccggcgtcc tcggccggat
    12961 gagcccgccc gacacccaca atgagggcca ggccgagttc aaccgcaacg gccccggctg
    13021 gaatgaggaa cagctgcgcc cgggcggatt catgttcggc accgtggtcc acgagctcgg
    13081 ccacggccac ggcatggccc acccgcacga caatggcggc cgctcgagcg tgatgcgcgg
    13141 cgtcgaatcc accggcggcg ccttcagcta cacgctcggc gcctacaatc tgaaccaggg
    13201 cgtcttcacc atgatgtcct atcaggacgg ctggcagagc tcgccctacg gcaactcgcc
    13261 ggatgacggc tatggctatc tcgccgggcc gatggcgctc gacattgcgg tgctccagga
    13321 caaatatggc gtcaacgaag agaccgccac gggcgacgac atctaccgcc tgaaggacgt
    13381 caacgccgcg ggcacctcct acgaggcgat ctgggatgcc ggcggccacg acctcgtcgc
    13441 ctatgacggc gcccgcgatt ccaagatcga ccttcgcgac gccaccttgc aatatgagc
//
LOCUS       DASVRK010000079         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_200318, whole genome shotgun sequence.
ACCESSION   DASVRK010000079 DASVRK010000000
VERSION     DASVRK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200318"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VF589_02720"
     CDS             <1..1311
                     /locus_tag="VF589_02720"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX8256522.1"
                     /translation="GFEEFFRRFGAPVPDGGGEAPSTQRGGSLGSGFIISPDGYIVTN
                     NHVISPARNGATVESITVTLTNRQEYEAELIGRDTASDLALLKIRASNLPYVRFGDST
                     RTRVGDWVVAIGNPFGLGGTVTAGIVSALHRSIQGAGAYDRYIQTDASINTGNSGGPM
                     FDINGNVIGINTALISPTGGNVGIGFAIPAEQARPVIDALRRGQRPQRGYIGVSLQPL
                     DEGIAESLGIERNRGELIRGVQAGGSAARAGIQQGDVVITVAGRPVTPDESLAYLVSQ
                     QPIGSRVPLELIRNGRRQVINVVIQERPTEEELARINNAEEAEVAETPEAQQSTGQRS
                     ARQSLGVTVQTLTPEIARTLRMTDTTARGVVIASVDPSSDAAAKGLRAGDVILSVNQR
                     AITTPEEAAAAVEAARSARRSSVLLLIRRGNTQPIYVGVELARR"
     gene            1466..>2366
                     /locus_tag="VF589_02725"
     CDS             1466..>2366
                     /locus_tag="VF589_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX8256523.1"
                     /translation="MSETDIFIGLGGGGAQSLPLSRANRHGLIAGATGTGKTVTLQGL
                     AESFSAAGVPVVAADVKGDLAGIAMAGSPTAKAHPALAARAAEIGLSDFAYSDNPAIF
                     WDVFGEAGHPVRATVSEMGPLLLARLMNLSEAQEGVLNIAFRLADEEGLLLLNLEDLQ
                     ALLLHCAERAGELSARYGNVTKPSVGAIQRQLLQLEAQGGGGFFGEPALDIADLMKVD
                     ELGRGFVSILAADRLMASPRLYATFLLWLLSELFEELPEVGDLDKPKLVFFFDEAHLL
                     FDDAPRALQDKIEQVVRLIRSKGV"
BASE COUNT          364 a          848 c          807 g          347 t
ORIGIN      
        1 ggcttcgagg agttcttccg gcggttcggc gccccggtgc cggacggcgg cggcgaggca
       61 cccagcacgc agcgcggcgg ctcgctcggc tcgggcttca tcatctcgcc ggacggctat
      121 atcgtcacca acaaccatgt gatctcgccg gcgcgcaacg gcgccaccgt cgaatcgatc
      181 accgtcaccc tcaccaaccg ccaggaatat gaggcggagc tgatcggccg cgacaccgcc
      241 tccgacctcg ccttgctcaa gatccgcgcg agcaacctgc cctatgtccg cttcggcgat
      301 tcgacccgca cccgggtcgg cgattgggtc gtggcgatcg gcaatccgtt cggcctcggc
      361 ggcaccgtca ccgccggcat cgtctcggcg ctgcaccgct cgatccaggg cgccggcgcc
      421 tatgaccgct acatccagac cgacgcgtcg atcaacaccg gcaattcggg cggaccgatg
      481 ttcgacatca acggcaacgt gatcggcatc aacaccgcgc tcatctcgcc gaccggcggc
      541 aatgtcggca tcggcttcgc catccccgcc gagcaggcgc ggccggtgat cgacgcgctg
      601 cgccgcggcc agcgcccgca gcgcggctat atcggcgtca gcctgcagcc gctcgacgaa
      661 ggcatcgccg aatcgctcgg catcgagcgc aatcgcggcg agctgatccg cggcgtccag
      721 gctggcgggt cggcggcgcg cgccggcatc cagcagggcg acgttgtgat cacggtggcc
      781 gggcggccgg tcacgccgga cgaatcgctc gcctatctgg tctcacagca gccgatcgga
      841 tcgcgcgtcc cgctcgaact gatccgcaac gggcggcgcc aggtcatcaa cgtggtcatc
      901 caggagcggc cgaccgagga agagctggcg cggatcaaca atgccgagga agcggaggtg
      961 gccgaaacgc cggaggcgca gcagtcgacc ggccagcgct cggcgcggca gagcctgggc
     1021 gtcaccgtcc agacgctgac gccggagatc gcccgtacgc tgcggatgac cgacacgacc
     1081 gcccgcggcg ttgtgatcgc ctcggtcgat ccgtcgagcg atgccgccgc caagggcctt
     1141 cgcgccggcg acgtcatcct gtcggtcaac cagcgcgcca tcacgacgcc ggaggaggca
     1201 gccgccgcgg tcgaggcggc gcggagtgcg cgccgcagct cggtgctgct gctcattcgc
     1261 cgcggcaaca cccagcccat ctatgtcggc gtggagctcg cgcgccgcta atcggctgct
     1321 cccgcgaggg agggcaggaa caggtgaagc ctccgggacg gtcagccgtg ccggaggctt
     1381 tccttttgcg cgagcggcgc aagctcggct atgcctcggc gccaacccgc cgctcctcga
     1441 gcgggagccg aggcaaggac ccagtttgag cgagacggac atcttcatcg gcctcggcgg
     1501 cggcggggcc caaagcctgc cgctgagccg cgccaaccgc cacggcctga tcgccggcgc
     1561 caccggaacc ggcaagacgg tgaccctgca agggctcgcc gaaagcttct ccgcggccgg
     1621 agtcccggtc gtcgcggccg acgtgaaggg cgaccttgcc ggcattgcga tggccggatc
     1681 gccgaccgcc aaggcccacc ccgcgctcgc cgcccgcgcc gccgagatcg ggctttcgga
     1741 ctttgcctat tccgacaatc cggcgatctt ctgggacgtg ttcggcgaag ccgggcaccc
     1801 ggtccgcgct accgtgtccg agatggggcc gttgctgctc gcgcgcctca tgaacctttc
     1861 ggaggcgcag gagggcgtgc tcaacatcgc cttccggctg gccgacgagg aagggctgct
     1921 actgctcaac ctcgaggatt tgcaggcgct gctgctccat tgcgccgagc gcgcgggtga
     1981 gctttccgcg cgctacggca acgtcaccaa gccgagcgtc ggcgcgatcc aacgccagct
     2041 gctccagctc gaggcgcagg gcggcggcgg cttcttcggc gagccggcgc tcgacattgc
     2101 cgatctcatg aaggtggacg agctcgggcg gggcttcgtc agcatcctcg ccgccgaccg
     2161 gctgatggcg agcccgcggc tctacgcgac cttcctgctg tggctgctgt ccgagctgtt
     2221 cgaggagctg cccgaggtcg gcgacctcga caagccgaag ctggtcttct tcttcgacga
     2281 ggcccatctg ctgttcgacg acgcgccgcg cgcgctccag gacaagatcg agcaggtggt
     2341 gcggctgatc cggtcgaagg gcgtcg
//
LOCUS       DASVRK010000080         4978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_573710, whole genome shotgun sequence.
ACCESSION   DASVRK010000080 DASVRK010000000
VERSION     DASVRK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573710"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            880..1260
                     /locus_tag="VF589_02730"
     CDS             880..1260
                     /locus_tag="VF589_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256524.1"
                     /translation="MPGRTRRDGWSPERQQAFILRLALCGCVNASARAVEMQARGAHK
                     LRERPGGESFAAAWDKAFEWGRESRIDHSIERALAGEIRSHHYRGRVCGEDIRFGDGL
                     SMAVLNALKPVYRDTDDKRFLRRR"
     gene            complement(1323..3206)
                     /locus_tag="VF589_02735"
     CDS             complement(1323..3206)
                     /locus_tag="VF589_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX8256525.1"
                     /translation="MLNLSAITVRLGGRTILDGATAALPPGSRVGLIGRNGAGKSTLM
                     RVVAGQLEADSGGIDMPRAARLGYIAQETPAGTATPFETVLAADLERAALLDEAETVT
                     DPHRIAEVHERLNAIAAHAAPARAARILVGLGFDEEMQQQPLDSFSGGWRMRVALAAL
                     LFSGPDLLLLDEPSNHLDLEATLWLENFLKGYKATMLIVSHERDLLNNVVDHILHLKQ
                     GKVTLYPGGYDAFEKQYAERLAQLESARANQAVQRARLQDYIARNSARASTAKQAQSR
                     AKALARMQPIAEVAADPSLSFDFPSPAELKPPLVNLDMAAVGYAPGQPVLSRVSLRLD
                     PTDRIALLGRNGNGKTTLARLLAAQLAPMEGEMRASGKLKVGYFTQYQVEELDREDTP
                     LQHMTRLMPKATPAAVRAQLGRFGFSGENATMQVARMSGGERARLALALITRDAPHML
                     ILDEPTNHLDVDAREALVQALNDYSGAVVVVSHDRHMLELTADRLVLVDGGTAREFAG
                     SLDDYTDLILGRTGEGGGAGKEAPKRSRKDERRAAAEARGASQSLRKAAEAAEAEIAR
                     LEARKREIEQAMFDPSAATAADARLTMTELMKLHAETGAKLAEAEARWLEASEAMERA
                     RAA"
     gene            complement(3366..4100)
                     /locus_tag="VF589_02740"
     CDS             complement(3366..4100)
                     /locus_tag="VF589_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX8256526.1"
                     /translation="MDGGEIRPVEGAALRGERLRYFDLVMAAFVTILLLSNVLGAGKV
                     AVVSLPGFGPVPFGAGILFFPVSYVIGDILTEVYGYARARRCIWAGFGALLFMALMAA
                     VVVALPPAADWGGQGAYEQVFGQVPRIVLASIAAFWAGEFANSYVLARMKVWTRGRHL
                     WSRTIGSTIVGQGIDSLIFYPLAFYGVWANDTLLAVLAVQFALKVGWEALLTPVTYVV
                     VGWLKRREGVDVYDRATDFTPFRAAL"
     gene            4161..4237
                     /locus_tag="VF589_02745"
     tRNA            4161..4237
                     /locus_tag="VF589_02745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4195..4197,aa:Arg,seq:tct)
     gene            complement(4273..>4978)
                     /locus_tag="VF589_02750"
     CDS             complement(4273..>4978)
                     /locus_tag="VF589_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8256527.1"
                     /translation="AFTDRTRLVIFNNPHNPTSRLFGRDELALLAAACVAHGAVAISD
                     EVWEHVLFDAAEFVPLSALPGMAERTVKVGSAGKIFALTGWKVGWAIAPAALSAPIMR
                     AHQYLTFATPPNLQSAIAYGLAKEDGYFTAMRSELAAARGFLDQGLRAAGFATLPAEG
                     TYFVSIDLAASGIALDDVSFCERAVEEAGVAAIPVSALYAEDPARHIVRLCFAKSRDT
                     LEAGIERLAAARAMFG"
BASE COUNT          771 a         1747 c         1699 g          761 t
ORIGIN      
        1 caagttcgcg atccacgata gcgtttagaa gctcgatcgc cggacgtggc aggccgaagt
       61 gcagcacggc atccttgtag tcagccggaa ggcgcgtttg gagccgcctt tcaacctctt
      121 ccagttcctt ttcgctgacg gcatcaggcg tgctgtcggg atgcgtctaa agcgcgatga
      181 agcggctcat gctcatcgct cggtcttagc ctttcacgac gggattgagg aagagcgcca
      241 atgtctcctt tccacccatt cggacattgg cgcgctcgct tctcgtaagg accccacctc
      301 gcagaagccg cctggggttc ggcgttgcat ctctcccgtt ccccggcgaa ggccggggtc
      361 cagcttccct ccgccttccc cggcggccga ggcacgggtg aaagctttcg ctggaatgtc
      421 gaggagctta cgctggaacg aaggggtgag cggccccgag cggttcgcag cggcgccgag
      481 ttgcccgaca tgcgatggat cgtcatgcca gcgaaagctg gcatctctct ttccttgagc
      541 gggggagaag aagggggatt ccagctttcg ctggaatgac ggatcaccgc gcccgttcac
      601 cgcctccgcc aattgcccgg cggaaatcgt tccccggcga aggccggggc ccagctccct
      661 tccgttcccc ggcgaaggcc ggggcccagc ttccctctcc cgtccgcccg aaccgaaaag
      721 ccctgaacga ccgcccgcac cggaacagaa aaagaacttt cctacacgcc ccgattcgag
      781 ccacgctccc cttatgcccg agtcggaccc tatccgctgc cgccgcggct ggcggcgctc
      841 accgcttcgc tgccgctcca cgagctcgcc ttcacgcccg tgccggggcg gacgcggcgc
      901 gacggctggt cgccggagcg gcagcaggcg ttcatcctcc ggctggcctt gtgcggctgc
      961 gtcaacgcct cggccagggc cgtcgaaatg caggcccgcg gcgcccacaa attgcgcgag
     1021 cggccgggcg gtgagagctt cgcagccgcc tgggacaagg cgttcgaatg gggccgcgag
     1081 agccggatcg accattcgat cgagcgcgcc ttggccggcg agatccgctc tcatcattat
     1141 cgcggccgcg tatgcggcga ggacatccgc ttcggcgacg gcctctccat ggccgtcctg
     1201 aacgcgctga agccggtgta ccgcgacacc gacgacaaac ggtttttacg gcgcagatga
     1261 gggtatttag tgtactttcg tgccctttac cggcgcccgc cagccgcccc ggcagccgcc
     1321 gttcaggcgg cccgggcccg ctccatcgcc tccgacgcct ccagccagcg cgcctcggcc
     1381 tcggccagct tggcgccggt ttcggcgtgc agcttcatca gctcggtcat ggtcagccgg
     1441 gcgtcggccg ccgttgccgc ggacgggtcg aacatcgcct gctcgatctc gcgcttgcgg
     1501 gcttccagcc gcgcgatctc ggcctcggcc gcctcggccg ccttgcgcag cgactggctc
     1561 gccccgcgcg cttcggccgc cgcgcggcgc tcgtccttgc ggctgcgctt cggcgcctcc
     1621 ttcccggccc cgcccccttc cccggtgcgg ccgaggatga ggtcggtata atcgtcgagg
     1681 ctgccggcga actcgcgcgc ggtgccgccg tcgaccagca ccagccggtc ggcggtgagc
     1741 tcgagcatgt ggcggtcgtg gctgaccacc accaccgcgc ccgaataatc gttgagcgcc
     1801 tggaccagag cctcgcgcgc gtcgacgtcg agatggttgg tgggctcgtc gaggatcagc
     1861 atgtgcggcg cgtcccgcgt gatcagcgcc agcgccagcc gggcgcgctc gccgcccgac
     1921 atcctggcga cctgcatggt cgcgttctcg ccggaaaagc cgaaccggcc gagctgcgcg
     1981 cgcaccgccg ccggcgtcgc cttcggcatc agccgcgtca tatgctggag cggcgtgtcc
     2041 tcccgatcca attcctcgac ttgatattgg gtgaaatagc cgaccttgag cttgcccgag
     2101 gcgcgcatct cgccttccat cggcgccagc tgcgcggcga gcagccgcgc cagcgtggtc
     2161 ttgccgttgc cgttgcggcc gagcaaggcg atgcggtcgg tcgggtcgag ccggaggctg
     2221 acccgggaca gcaccggctg gccgggcgcg tagccgaccg cggccatgtc gagattgacc
     2281 agcggcggct tcagttcggc cgggctcgga aagtcaaagc tgaggctcgg atcggcggcg
     2341 acttcggcga tcggctgcat ccgcgccagc gccttggcgc gcgactgggc ctgcttggcg
     2401 gtgctggcgc gggccgagtt gcgggcgata taatcctgca gccgcgcgcg ctgcaccgcc
     2461 tggttggcgc gcgccgattc gagctgcgcc agccgctcgg catattgctt ctcgaacgcg
     2521 tcatagccgc ccggatagag cgtgaccttg ccctgcttga ggtgaaggat gtggtcgacg
     2581 acgttgttca acaggtcgcg ctcgtggctg acgatcagca tcgtcgcctt gtagcccttg
     2641 aggaaattct cgagccagag cgtcgcctcg aggtcgagat ggttggatgg ctcgtcgagc
     2701 agcagcaggt ccggcccgga gaacagcaag gcggcgagcg cgacccgcat ccgccagccg
     2761 cccgaaaagc tgtcgagcgg ttgctgctgc atctcctcgt cgaagccgag cccgaccaga
     2821 atgcgcgcgg cgcgcgccgg ggcggcgtgg gcggcgatcg cgttcagccg ctcgtggacc
     2881 tcggcgatgc ggtgcggatc ggtgacggtc tcggcctcgt cgagcagggc cgcgcgctcg
     2941 aggtccgccg ccagcaccgt ctcgaacggc gtcgccgttc ccgccggcgt ctcctgcgcg
     3001 atatagccga gccgggccgc gcgcggcatg tcgatgccgc cggaatcggc ctcgagctgc
     3061 ccggccacga cccgcatcaa ggtcgacttg ccggcgccgt tgcggccgat cagcccgacg
     3121 cggctccccg gcggcagcgc ggcggtggcg ccgtcgagga tggtacggcc gccgagccgc
     3181 acggtgatcg cgctgagatt gagcatggcg cgcccttagc ccggacgggc gcttctgtca
     3241 ggggggatat tgctagagca cgtcggcgaa catgaaccac agcatggcga gccaggccgc
     3301 gaccgcggcc aggcccaaca gccaccaata gcgggccggc cgctcctcct cgccgccggg
     3361 ctcgctcaca aagccgcccg gaacggcgtg aagtcggtcg cgcggtcgta gacgtcgacg
     3421 ccctcgcgcc gtttgagcca gccgaccacc acataggtga ccggggtcag cagcgcctcc
     3481 cagccgacct tgagcgcgaa ctgcacagcc agcaccgcca gcaaggtgtc gttcgcccag
     3541 acgccgtaga aagcgagcgg gtagaagatc aggctgtcga tcccctggcc cacgatcgtc
     3601 gagccgatgg tgcggctcca cagatgcctg cccctggtcc acaccttcat ccgcgcaagc
     3661 acgtagctat tggcgaactc gcccgcccag aaggcggcga tcgaggccag cacgatccgc
     3721 ggcacctggc cgaacacctg ctcgtaagcc ccctgccccc cccagtccgc agccggcggc
     3781 agcgccacca ccaccgccgc catcaacgcc atgaacagca aagctccgaa cccggcccag
     3841 atgcagcgcc gcgcgcgggc atagccgtag acctcggtca ggatgtcgcc gatcacatag
     3901 gagaccggga agaacaggat gccggccccg aacggcaccg ggccgaaacc gggcaggctc
     3961 accaccgcga ccttgccggc gccgagcacg ttggagagca agaggatggt gacgaacgcc
     4021 gccatgacga ggtcgaaata gcgcagccgc tcgccccgca aggcggcgcc ttcgaccggc
     4081 ctgatctctc ccccgtccac ggcggccacg actagcgctg ttccgctcgc cttgaaagcg
     4141 cgctagagcg aagccgtcgc gcgcccgtag ctcagccgga tagagcacga gccttctaag
     4201 cttggggtcg caggttcgag tcctgccggg cgcgccagtg ttccggcagc tcggcgaggc
     4261 gctctgaccg cgctagccga acattgcccg ggccgcggcg aggcgttcga tgccggcttc
     4321 gagcgtgtcg cggctcttgg cgaagcagag gcggacgatg tggcgggcgg gatcctcggc
     4381 gtagagcgcc gagaccggga tggcggccac gccggcttcc tcgaccgcgc gctcgcagaa
     4441 ggagacgtcg tcgagcgcga tgccggatgc ggcgagatcg atcgacacga agtaggtgcc
     4501 ctcggccggg agcgtcgcga agccggcggc gcggaggccc tgatcgagga agccgcgcgc
     4561 ggcggcaagc tcgctccgca tggcggtgaa atagccgtcc tccttggcca aaccgtaggc
     4621 gatcgcggac tggaggttgg gcggggtggc gaaggtcaga tattggtgcg cgcgcatgat
     4681 cggcgccgac agcgccgccg gcgcgatcgc ccagccgacc ttccagccgg tgagcgcgaa
     4741 gatcttgccc gccgagccga ccttgacggt acgctcggcc atgcccggca gcgccgagag
     4801 cggcacgaat tcggccgcgt cgaacagcac atgctcccag acctcgtcgc tgatcgcgac
     4861 cgcgccgtgc gcgacacagg cggcggcgag cagcgccagc tcgtcgcggc cgaacaggcg
     4921 cgaggtgggg ttatgagggt tgttgaagat gacgaggcgg gtgcggtcgg tgaaggcg
//
LOCUS       DASVRK010000081        12384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_387088, whole genome shotgun sequence.
ACCESSION   DASVRK010000081 DASVRK010000000
VERSION     DASVRK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12384
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387088"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF589_02755"
     CDS             complement(<1..623)
                     /locus_tag="VF589_02755"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX8256528.1"
                     /translation="MTRHLRLGVNIDHVATIRNARGGDHPDPVRAAFAAAAAGADGIT
                     AHLREDRRHITDDDISRLMERLTIPLNLEMAATEEMLEIALRHRPHAACIVPEKREER
                     TTEGGLDAAGQDNHLMPYIDRLSGAGIRVSLFIEPTEEQVDAAIRLGAPVVEFHTGRY
                     AHLEGEARAEELRRIADAAALAWKNGIEPHAGHGLTFDNVQPVAAIP"
     gene            complement(620..1228)
                     /gene="pyrE"
                     /locus_tag="VF589_02760"
     CDS             complement(620..1228)
                     /gene="pyrE"
                     /locus_tag="VF589_02760"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX8256529.1"
                     /translation="MTEDEILAEFRAVEALLEGHFILSSGLHSPRYLQCARVLMDPAR
                     ASRLASALARTLPLEIKSKLELVISPAMGGVIIGHEMGRSLGLPAMFLERPAGTFELR
                     RGFAVEPGQKVLLVEDVVTTGLSSKEAMKAVRQAGAEVIAAAALIDRSNGLADLGVPF
                     FPLIALDVPVYEPQNLPAALAALPPEKPGSRKPVPSEAEGAA"
     gene            1408..2622
                     /gene="coxB"
                     /locus_tag="VF589_02765"
     CDS             1408..2622
                     /gene="coxB"
                     /locus_tag="VF589_02765"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8256530.1"
                     /translation="MKTLLKVIALAGAIGLSPAAAPAQDANLAQPKQALTAQPQAMTG
                     AAARPAPQSTAGATTQPEQVSDPALATPAEEEPSNAQIAQTTQTAQALGPRSVPQIGV
                     GQPDGRMNLQDQFTPIGEEAAWFHNVILLPVITAISVFVLILMLYVIVRYRRSANPVP
                     SRTTHNTLIEVLWTLVPVIVLVVIAVPSIRLLANQYSPPTPDLTVKVIGNQWYWTYQY
                     PDNGDFEIVSNGLTDAQAKERGEPRQLAVDERMVVPAGATVKLIITSNDVIHAFGVPA
                     FWVKMDAVPGRLNESWFKVDKPGLYYGQCYELCGARHAYMPIAVEVVPPAQYAAWVAS
                     KGGTMPNKVRPTSSDATQNSPISNPGAGPATPTAGGSATETSPEPTNMIDATIAPPVS
                     SQGAAESRRGSE"
     gene            2627..4294
                     /gene="ctaD"
                     /locus_tag="VF589_02770"
     CDS             2627..4294
                     /gene="ctaD"
                     /locus_tag="VF589_02770"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX8256531.1"
                     /translation="MTDTTANAVHFEAGHAHAGHHDADHKPGFFARWFMSTNHKDIGT
                     LYLIFAIVAGIIGGAISGLMRAELAEPGVQYLQAWAGAGATFDEGLHLWNVLITAHGL
                     IMVFFMVMPAMIGGFGNWFVPIMIGAPDMAFPRMNNISFWLLVPAFILLLGSSFVPGG
                     TGLGAGTGWTVYAPLSTSGSSGPAVDMAILSLHLAGASSILGAINFITTIFNMRAPGM
                     TLHKMPLFVWSVLVTAFLLLLALPVLAGAITMLLTDRNFGTSFFDASGGGDPVLYQHL
                     FWFFGHPEVYIMILPGFGIVSQIVATFSRKPVFGYLGMAYAMVAIGVVGFVVWAHHMF
                     TVGMDVNTKMYFTAATMVIAVPTGVKIFSWIATMWGGSLSFQTPMMWALGFIFMFTVG
                     GVTGVVLANGGVDNEMHDTYYVVAHFHYVLSLGAVFGLFAGWYYWFGKMWGRDYNELL
                     GQLHFWVFFVGVNILFFPMHFLGLQGMSRRVPDYPDAYAGWNEWATVGYMIMAVGMIF
                     FFVNILWSLLFGKRTPDNPWGEGATTLEWTLSSPPPFHQFETLPKVD"
     gene            4442..5353
                     /locus_tag="VF589_02775"
     CDS             4442..5353
                     /locus_tag="VF589_02775"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX8256532.1"
                     /translation="MSGIVLQQTQAVSADWRDFVALTKPRVMTLVVFTGLCGLAAAPT
                     QIHPVLAFTAILCIALGAGAAAALNQWYEADIDALMKRTRNRPLPAGRMDRDTALHFG
                     VGLGAFSVLLMGVATNWFAAAVLAVSILFYVLVYTVWLKRRTPQNIVIGGAAGAFPPV
                     IGWAAVTGDVGLLPFLLFLLIFLWTPPHFWALSLFVRSDYANAGVPMLPVVSGPKVTR
                     QQIALYTLPMAVCAVAPWPLGLTGPVYGLAAAALSLAFVVLAAQVLANRATEPAAMKP
                     EKRLFAFSILYLFAVFGALVIDRWLFA"
     gene            5350..5475
                     /locus_tag="VF589_02780"
     CDS             5350..5475
                     /locus_tag="VF589_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256533.1"
                     /translation="MTPEDPLRARQRSRARLMALLLGAFVVLMFAITIAKMSGGE"
     gene            5472..6020
                     /locus_tag="VF589_02785"
     CDS             5472..6020
                     /locus_tag="VF589_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX8256534.1"
                     /translation="MTTLARKNGRTAALAALLVAAMVGLAFASVPLYRLFCQVTGFGG
                     TPLRADQVTVQATGKLINVRFDANTNSALPWEFSPERNVERVAVGARDMAFYTARNLS
                     DKPVTGTATFNVTPAQAGQYFTKIQCFCFTEQTLKPGEQVRMPVVFQVDPKILEDPDA
                     RRISEITLSYTFYPVDQAKTDG"
     gene            6106..6921
                     /locus_tag="VF589_02790"
     CDS             6106..6921
                     /locus_tag="VF589_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX8256535.1"
                     /translation="MAGAKNHQFHILPPSIWPFIGSFSALAMAAGAIMWMHDMAGGGY
                     VFFAGLVAVLFVMFSWWVDVVREARSGDHTPVVQLHLRYGMILFIASEVMFFVAWFWA
                     YFNAALFPSPVESIGGVWPPEGMHVLDPFGWPLLNTLILLCSGTTVTWAHHGVIHGDR
                     DALKKGLWATIGLGIVFTGVQAYEYAHAPFGFADNIYTSAFFMATGFHGFHVIVGTIF
                     LIVCLARAYKGDFTPRQHFGFEAAAWYWHFVDVVWLFLFLAIYVWGGWGAATH"
     gene            6938..7462
                     /locus_tag="VF589_02795"
     CDS             6938..7462
                     /locus_tag="VF589_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEX8256536.1"
                     /translation="MRIPFLPTVIVGAAVAVMIGLGLWQLQRAEWKERLIAEAAAPGP
                     RSATLTCTVDARPAARAGRSRGGEIGYRFLVPCRGAEGTLTLDLGWSKRPNALPHLRA
                     TRTFTGTFAGGQAAANRLLVLATPVPPLEPSAVPSAADLPNNHLLYALQWFFFAAAAA
                     VIYALALRRRRAGG"
     gene            7570..8796
                     /locus_tag="VF589_02800"
     CDS             7570..8796
                     /locus_tag="VF589_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8256537.1"
                     /translation="MMQLTILPNGLRVASREMPGLETAAVGLYADTGSRYEPARLGGL
                     AHLFEHMVFKGAGGRSARAISEAIEDVGGDLNAATDRDSTNFMASVLAEHVGLGVELI
                     ADLILQPHFYKTDLEREKDVVLQELGEARDTPNDIIFDDLWSAAFADQPLGRSVLGEE
                     ESIAAITVDDLHDWRRGQYRAESLALVAAGKVEHAALVELAMRYLSELPSGEIAPPLP
                     AHFTGGDRVGRASSDQAHLALAFPGPAQLDGEHYAARLFADAVGGGASSRLFQQLREE
                     RGLAYSVYASLSGYRDTGLFTVYAATARRQSAASARLIEQILTEAAAALTEREVDRAR
                     TQAKAGLLMAMESPWGQANYVARQLALHGRLVEPREIIARLDAVTADEVRAAGAKMLA
                     GPRARATIGFPAARAA"
     gene            8793..9659
                     /locus_tag="VF589_02805"
     CDS             8793..9659
                     /locus_tag="VF589_02805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8256538.1"
                     /translation="MTPATLIAEPWSHYGLVDSGHGRKLERYGRYSFVRPEPQAMWAP
                     ALDAWDPDGEFIPGSDEEGGGRWQFARPVPREGWEMAWEEVRFRAQCTPFRHLAFFPD
                     MAPQWAWMRERLGPGSEALNLFGYTGVGTLAMSAAGASLVHVDASKKSVEAGKENAAL
                     AGLAANPIRWIVEDASKFAAREVRRGRRYDGIILDPPKFGRGPAGEVWRLEENLPALV
                     ADCARLLDADSRFLVLTVYAVRMSALAIGELLSQALAGLGGRVECGDMAVREETRGLL
                     LPTAIFARWSKD"
     gene            complement(9656..10297)
                     /locus_tag="VF589_02810"
     CDS             complement(9656..10297)
                     /locus_tag="VF589_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX8256539.1"
                     /translation="MIALVLRELRIAASGGNAMLPLAFFLLVAALFPFAVGPDRPLLV
                     KAGGGALWMAALLAALLPVERLVEPDRAAGVLDQLALRGFSEEAVALAKLAGHWLSFG
                     PPLMLAAVPAAALMKLSAETLARVELGLLVGTPGLAALTLTVAALTAGLKGTGALAGL
                     LLLPLAVPILIFGAGLVGGNGGTGALQLLAAASLLLVAAAPFAAGAAIRAARD"
     gene            complement(10294..10863)
                     /gene="ccmA"
                     /locus_tag="VF589_02815"
     CDS             complement(10294..10863)
                     /gene="ccmA"
                     /locus_tag="VF589_02815"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX8256540.1"
                     /translation="MSGPPLLVLEDVACRRGGRLLFEGLNLRLQAGGAAVVTGPNGAG
                     KSSLLRLVAGLLDAAAGRIERATAALADNRAALDERLTLERALAFWAGLDRVEVGPAM
                     AAMGLAELARVPVRMLSTGQRKRAMLARVLAGPARLWLLDEPLNGLDADGQARLARAI
                     AEHRAAGGAVLAATHQPLGLDQADEVALG"
     gene            complement(10860..11273)
                     /locus_tag="VF589_02820"
     CDS             complement(10860..11273)
                     /locus_tag="VF589_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX8256541.1"
                     /translation="MSDRQTFAPDAAEIERLAEAAIARLPEPFRRHLDGVVLRIDDFA
                     EEAVLAELGIEDPFDLTGLYTGRPIGERSSMASGELPAMIHLYRRPLLDEWAEDGVSL
                     EDLITHVIVHEVGHHFGFSDEDMHAIEEAAEGDGE"
     gene            11328..11642
                     /locus_tag="VF589_02825"
     CDS             11328..11642
                     /locus_tag="VF589_02825"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX8256542.1"
                     /translation="MRTLSEEEAAEALAGLPGWSFDAGRGAILKSFRFAGFAEAFGFM
                     TAVAIAAEKADHHPDWSNSWNRVDILLTTHSAGGLTARDLDLAGRIEALAGSLALEAP
                     GD"
     gene            11666..11756
                     /locus_tag="VF589_02830"
     tRNA            11666..11756
                     /locus_tag="VF589_02830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11700..11702,aa:Ser,seq:gga)
BASE COUNT         1867 a         4342 c         4157 g         2018 t
ORIGIN      
        1 tgcgggatgg cggccaccgg ttgcacgttg tcgaaggtga ggccgtggcc ggcatggggc
       61 tcgatgccgt tcttccacgc caaagcggcg gcgtcggcga tgcggcgcag ctcctcggcg
      121 cgcgcctcgc cttcaaggtg cgcgtagcgg ccggtgtgga actcgaccac cggggcgccg
      181 aggcgaatgg cggcgtccac ctgttcttcg gtgggctcga tgaacaggct gacgcggatg
      241 ccggcgccgg acagccggtc gatatagggc atcagatgat tgtcctggcc cgccgcgtcg
      301 aggccgccct cggtggtgcg ctcctcgcgc ttttcgggaa cgatgcaggc ggcgtgcggg
      361 cggtggcgga gcgcgatctc cagcatctcc tcggtcgccg ccatttcgag gttgagcggg
      421 atggtgagcc gctccatcag ccgcgagata tcgtcgtcgg tgatgtgccg ccggtcctcg
      481 cgcaggtgcg cggtgatgcc gtcggcgccc gccgcggcgg cggcgaaggc ggcgcgcacg
      541 ggatcgggat ggtcgccgcc ccgggcgttc cggatcgtcg cgacatggtc gatgttgacg
      601 cctaggcgga ggtggcgcgt cacgccgccc cttcggcttc gctcgggacg ggcttgcggc
      661 tgcccggctt ttcgggcggc agcgccgcca acgcggccgg caggttctgc ggctcgtaga
      721 ccggcacgtc gagcgcgatc agcgggaaga aaggcacgcc gaggtcggcg agtccgttcg
      781 accggtcgat gagggcggcg gcggcgatca cctccgcgcc ggcctgccgc accgccttca
      841 tcgcctcctt ggaggacagg ccggtggtga cgacgtcctc gaccagcagc accttctggc
      901 ccggctcgac ggcgaagccg cggcgcagct cgaacgtgcc ggccggccgc tcgaggaaca
      961 tggcgggcag gccgagcgat cggcccatct catggccgat gattaccccg cccatcgccg
     1021 gcgagatcac cagctccagc ttcgacttga tctcgagcgg cagcgttcgg gccagcgccg
     1081 aagcgagccg ggaggcgcgg gcgggatcca tcagcacccg cgcgcattgc aggtagcgcg
     1141 ggctgtggag cccggaggaa aggatgaaat gcccttccag cagcgcctcc accgcccgga
     1201 attcggccag gatttcgtct tcggtcatgg ctcctcccgg gcgcggcgcg gctgcccgcc
     1261 agataggccg gagcggcctt cttctcaagc tttccgaaaa acctacctac ggcttgaggc
     1321 cggcccatcc gctgctataa ggcgccgcga cttagcctgt cccgaccgaa tccatcccgc
     1381 tcgccctggg cggcccaagg ggtttagatg aagactctgc tcaaagtgat cgccctggcc
     1441 ggagccatcg gcttgtcccc ggcggccgcg cccgcgcagg atgcgaacct cgcccagccc
     1501 aagcaggcgc tgaccgccca gccgcaggcg atgaccggcg cggcggcgcg cccggccccg
     1561 caatcgacgg cgggcgccac gacccagccc gagcaggtgt ccgaccccgc gctcgccacg
     1621 ccggccgagg aagagcccag caacgcccag atcgcgcaga ccacccagac cgcgcaggcg
     1681 ctcgggcccc gctcggtgcc gcagatcggc gtcggccagc ccgacggccg catgaacctc
     1741 caggaccagt tcaccccgat cggcgaggag gcggcctggt tccacaacgt catcctgctg
     1801 ccggtgatca cggcgatctc ggtgttcgtg ctgatcctga tgctctacgt gatcgtccgc
     1861 taccggcgca gcgccaatcc ggtgccgtcc cgcaccaccc acaacacgct cattgaagtg
     1921 ctgtggacgt tggtgccggt gatcgtgctg gtggtgatcg cggtgccctc gatccggctg
     1981 ctcgccaacc aatattcgcc gcccacgccc gacctcaccg tcaaggtgat cggcaaccaa
     2041 tggtattgga cctaccaata tcccgacaat ggcgatttcg agatcgtctc gaacggcctc
     2101 accgacgccc aggccaagga gcgcggcgaa ccgcgccaac tggcggtcga cgagcgcatg
     2161 gtggtgccgg ccggcgcgac cgtgaagctg atcatcacgt ccaacgacgt catccacgcc
     2221 ttcggcgtcc ccgccttctg ggtgaagatg gatgccgtcc ccggacgcct caacgagagc
     2281 tggttcaaag tcgacaagcc gggcctatat tacggacaat gctacgaatt atgcggagcc
     2341 cgccacgcct atatgccgat cgccgtggaa gtggtgccgc cggcgcaata tgccgcctgg
     2401 gtggcctcca agggcggcac catgcctaac aaggtgcggc cgacctcgtc cgacgcgacc
     2461 cagaactcgc cgatctccaa ccccggggcc gggccagcga ctccgaccgc gggcggcagc
     2521 gcgaccgaga ccagccccga accgaccaac atgatcgacg ccaccatcgc gcccccggtc
     2581 tcgagccagg gcgccgccga atccagacgc ggaagcgaat aagagcatga ccgacaccac
     2641 tgccaacgcc gtccatttcg aggcgggcca cgcccatgcc ggccatcacg acgccgatca
     2701 caagccgggc ttcttcgcgc gctggttcat gtctaccaac cacaaggaca tcggcacgct
     2761 ctatctgatc ttcgcgatcg tcgcggggat catcggcggc gccatctccg gcctgatgcg
     2821 ggccgaattg gccgagcccg gggtccagta tcttcaggcc tgggccggcg ccggcgcgac
     2881 cttcgacgag ggcctccatc tctggaacgt gctgatcacc gcccatggcc tgatcatggt
     2941 gttcttcatg gtgatgccgg cgatgatcgg cgggttcggc aactggttcg tcccgatcat
     3001 gatcggcgcg cccgacatgg ccttcccgcg gatgaacaat atcagcttct ggctgctcgt
     3061 ccccgccttc atcctgctct tgggctcctc cttcgtcccc ggcggcaccg gcctcggcgc
     3121 cggcaccggc tggaccgtct acgcgccact ctcgacctcc gggtcctcgg ggcccgcggt
     3181 cgacatggcg atcctgtcgc tccacctcgc gggcgcttcc tcgatcctgg gcgcgatcaa
     3241 cttcatcacg accatcttca acatgcgcgc gccgggcatg accctgcaca aaatgccgct
     3301 gttcgtgtgg tcggtgctgg tcaccgcgtt cctgctgctg ctggcgctgc cggtgctggc
     3361 gggcgcgatc accatgctgc tcaccgaccg caatttcggc acgtcattct tcgacgcgtc
     3421 cggcggcggc gacccggtcc tctaccagca tctgttctgg ttcttcggcc accccgaagt
     3481 ctatatcatg atcctgccgg gcttcggcat cgtcagccag atcgtcgcga ccttcagccg
     3541 caagccggtg ttcggctatc tcggcatggc ctatgcgatg gtggcgatcg gcgtggtcgg
     3601 cttcgtcgtg tgggcgcacc acatgttcac cgtcggcatg gacgtgaaca ccaagatgta
     3661 cttcaccgcc gccacgatgg tgatcgcggt gccgaccggc gtgaagatct tctcctggat
     3721 cgccaccatg tggggcggct cgctgtcgtt ccagaccccg atgatgtggg ctttgggctt
     3781 catcttcatg ttcacggtcg gcggcgtcac cggcgtcgtg ctcgccaacg gcggcgtcga
     3841 taacgagatg cacgacactt attatgtggt ggcccatttc cactacgtgc tctcgctcgg
     3901 ggcggtgttc gggctgttcg ccggctggta ttactggttc ggcaagatgt ggggccgaga
     3961 ttataacgag ctgctcggac agctccattt ctgggtcttc ttcgtcggcg tgaacatcct
     4021 gttctttccg atgcacttcc tcggcctgca aggcatgtca cggcgcgtcc ccgattatcc
     4081 ggacgcgtac gccgggtgga acgagtgggc caccgtcggc tatatgatca tggcggtcgg
     4141 catgatcttc ttcttcgtca acattctctg gtcgctcctg ttcggcaagc gcacgccgga
     4201 caatccctgg ggcgaaggcg cgacgacact cgaatggacc ttgtcgagcc cgccgccctt
     4261 ccaccagttc gagacgctgc cgaaggtcga ttgacgcgcc ctcccctccc gcccgcggga
     4321 ggggaaccag gagtgagcgg cgccccgctg gccgcgctcc gatccattga gaattccgcc
     4381 cccggcacat cccgcaagca ggagggacta tcgccggggt gacgtggcag gtgaaactga
     4441 tatgtctggc atcgtgctgc aacagactca ggccgtttcc gccgattggc gggacttcgt
     4501 ggcgcttacc aagccgcggg tgatgacgct cgtcgtcttc accggcctgt gcggcctcgc
     4561 cgccgcgccg acccagatcc acccggtgct cgcctttacc gccatcctct gcatcgcctt
     4621 gggcgcgggg gcggcggcgg cgctcaacca atggtatgag gccgacatcg acgctttgat
     4681 gaagcgcacc cgcaaccggc cgctgcccgc cggccggatg gaccgcgaca ccgcgctcca
     4741 cttcggggtc ggcctcggcg ctttctcggt gctgctgatg ggggtggcga ccaactggtt
     4801 cgcggccgcg gtgctggccg tctcgatcct gttctacgtt ttggtctaca ccgtctggct
     4861 gaagcggcgg accccgcaga acatcgtcat tggcggcgcg gcgggcgcgt tcccgccggt
     4921 gatcggttgg gcggcggtga ccggcgacgt cggcctgctc ccctttctgc tgttcctgct
     4981 gatcttcctg tggacgccgc cgcatttctg ggcgctgtcg ctgttcgtcc gctccgatta
     5041 cgccaatgcc ggggtgccga tgcttcccgt cgtgtccggc ccaaaggtca cccggcagca
     5101 gatcgcgctc tacacgctgc cgatggcggt ctgcgcggtc gccccctggc cgctcggcct
     5161 caccgggccg gtctacggcc tcgccgcggc ggcgctcagc ctggccttcg tcgtgctggc
     5221 cgcccaggtg ctcgccaacc gcgcgaccga gccggccgcg atgaagcccg aaaagcgcct
     5281 gttcgccttt tcgatcctct atctgttcgc cgtgttcggc gctttggtca tcgaccggtg
     5341 gctgttcgcg tgacgcccga ggacccgctc cgcgcccgcc agcgcagccg cgcccggctg
     5401 atggctttgc tgctcggcgc cttcgtggtg ctgatgttcg cgattaccat cgccaagatg
     5461 agcggcggcg aatgacgacg ctcgcaagga agaacgggcg gacggcggcg ctcgcggccc
     5521 tgctggtggc ggcgatggtc ggcctcgcct tcgccagcgt accgctctac cgcctgttct
     5581 gccaggtcac cggcttcggc gggaccccgc ttcgcgccga ccaggtcacc gtccaggcca
     5641 ccggcaagct gatcaacgtc cgcttcgacg cgaacaccaa ttcggcgctg ccgtgggagt
     5701 tcagcccgga gcgcaacgtc gagcgggtcg cggtcggcgc gcgcgacatg gccttctaca
     5761 cggcccggaa cctgtccgat aagccggtca ccggcaccgc caccttcaac gtcacgccgg
     5821 cccaggccgg gcaatatttc accaagatcc aatgcttctg cttcaccgag cagacgctga
     5881 agcccgggga gcaagtccgc atgccggtcg ttttccaggt cgacccgaag atattggagg
     5941 atcccgacgc gcgcaggatc agcgaaatca ccctgagcta caccttctac ccggtggacc
     6001 aggcaaaaac ggacggttag aggaccccca gttccccgcc atgccggggc cacgagagag
     6061 actggatacc ggcgctggcc ggtacgcaag gacggaaacg agaatatggc cggagcgaag
     6121 aaccaccaat tccacatcct gccgccgagc atctggccgt tcatcggcag cttctcggcg
     6181 ctcgccatgg cggccggggc gatcatgtgg atgcacgaca tggccggcgg cggctatgtc
     6241 ttcttcgccg ggctggtggc ggtgctgttc gtgatgttca gctggtgggt ggacgtggtc
     6301 cgcgaggcgc ggtccggcga ccatacgccg gtcgtccagc ttcacctgcg ctacggcatg
     6361 atcctgttca tcgcgtcgga agtgatgttc ttcgttgcct ggttctgggc ctatttcaac
     6421 gccgctttgt tcccctcgcc ggtcgaatcg atcggcggcg tgtggccgcc cgagggcatg
     6481 cacgtgctcg acccgttcgg ctggccgctg ctcaacacgc tcattctgct ctgctcaggc
     6541 accaccgtca cctgggcgca ccatggcgtg atccacggcg atcgcgacgc gctcaagaag
     6601 gggctgtggg cgacgatcgg cctcggcatc gtcttcaccg gggtgcaggc ctatgaatat
     6661 gcccacgcgc cgttcggctt tgccgacaat atctacacct cggctttctt catggcgacc
     6721 ggcttccacg gcttccacgt catcgtcggc acgatcttcc tgatcgtctg cctggcccgc
     6781 gcctataaag gcgacttcac cccgcgccag catttcggct tcgaggccgc cgcctggtat
     6841 tggcatttcg tcgacgtcgt ctggctgttc ctgttcctcg ccatctacgt ctggggcggc
     6901 tggggcgcgg cgacgcactg atccggcccg ccgcctgatg cggattccgt ttctccccac
     6961 cgtgatcgtc ggcgcggcgg tggcggtgat gatcgggctc ggcctctggc agctccagcg
     7021 cgctgagtgg aaggagcggc tgatcgcgga ggcggccgcg ccggggccgc gtagcgccac
     7081 cctcacctgc acggtcgacg cgcggcccgc cgcgcgcgcc ggccgaagcc gcggcggcga
     7141 gatcggctat cgcttcctgg tcccatgccg cggcgcggag gggacgctga ccctggacct
     7201 cggctggtcg aagcgcccga acgccctccc ccacctccgc gcgacccgca ccttcaccgg
     7261 cacctttgcg ggcggccagg ccgccgcgaa ccgcctgctc gtgctcgcaa cgccggtccc
     7321 gccgctggag ccgagcgcgg tcccgagcgc cgccgatctc cccaacaacc acctcctcta
     7381 cgcgctgcaa tggttcttct tcgccgccgc cgccgccgtc atctacgccc tggcgctgcg
     7441 gcggcggcgg gccgggggat agccggctcc gcggccgcca gggggccgtt acgtgcaagg
     7501 ttcgacaagt tcaccctcct gccggtgcgg ttgaagccgg agcggccagg gactaagtgc
     7561 gcccgaccca tgatgcagct caccatcctt cccaacggcc tccgcgtcgc cagccgcgag
     7621 atgccgggcc tcgagacggc ggcggtgggg ctctatgccg ataccggctc gcgctacgag
     7681 ccggcgcggc tcggcggcct cgcccacctg ttcgagcata tggtgttcaa gggcgccggc
     7741 ggccgctcgg cgcgcgcgat cagcgaggcg atcgaggatg tcggcggcga cctcaacgcc
     7801 gccaccgacc gcgattcgac gaacttcatg gcctcggttc tggccgaaca tgtcgggctc
     7861 ggagtcgagc tcatcgccga cctcatcctc cagccccatt tctacaagac cgatctcgag
     7921 cgcgagaagg acgtcgtcct gcaggaactg ggcgaggcgc gggatacgcc gaacgacatc
     7981 atcttcgacg atctgtggtc ggccgccttc gccgaccagc cgctcggccg gtccgtgctg
     8041 ggcgaggagg agagcatcgc ggcgattacc gtcgacgatc tccacgactg gcggcgcggc
     8101 cagtatcggg cggaaagcct cgctctggtc gccgccggga aggtcgagca tgccgcgttg
     8161 gtcgagctcg ccatgcgcta tctcagcgag cttccgtcgg gcgagatcgc tccgcctttg
     8221 cccgcccact tcaccggcgg cgaccgggtc ggacgcgcct cctccgacca ggcccatctc
     8281 gcgctcgcct ttccggggcc cgcgcagctc gacggcgaac attatgcggc gcggctgttc
     8341 gccgatgcgg tcggcggcgg cgcttcgtca cggctgttcc agcaattgcg cgaggagcgc
     8401 gggctggctt attcggtcta tgcgagcctt tccggctatc gcgacaccgg cctgttcacc
     8461 gtctatgccg ccaccgcccg gcgccagtcg gccgcctcgg cccgcctgat cgaacagatc
     8521 ctcaccgagg ccgccgcggc gctgaccgag cgcgaggtcg accgcgcccg cacccaggcc
     8581 aaggccgggc tgctgatggc gatggagagc ccgtggggcc aggccaatta cgtcgcccgc
     8641 cagctcgcgc tccacggccg gctggtcgag ccgcgcgaga tcatcgcccg gctcgacgcg
     8701 gtcacggccg acgaggtccg cgcggccgga gcgaagatgc tggccggccc gcgcgcccgc
     8761 gccacgatcg gcttcccggc cgcccgcgcc gcgtgacgcc cgcgaccctg atcgcggagc
     8821 cttggagcca ttacgggctc gtcgattcgg gtcatgggcg caagctcgag cgctacggcc
     8881 gctacagctt cgtccggccg gaaccgcagg cgatgtgggc gccggcgctc gacgcctggg
     8941 acccggacgg cgagttcatc ccgggctccg acgaagaagg cggcggccgc tggcaattcg
     9001 cccggccggt gccgcgcgag ggctgggaga tggcgtggga ggaggtccgc ttccgcgccc
     9061 aatgcacgcc cttccgccac ctcgctttct tccccgatat ggccccgcaa tgggcgtgga
     9121 tgcgcgagcg gctcggcccc ggcagcgaag cgctcaacct gttcggctat accggggtcg
     9181 gcacgctcgc catgtcggcc gcgggcgcga gcctcgtcca tgtcgacgct tcgaaaaaat
     9241 ccgtcgaggc gggcaaggag aatgccgcgc tggccgggct cgccgccaac ccgatccgct
     9301 ggatcgtcga ggatgcgagc aaattcgcgg cgcgcgaggt ccggcgcggg cggcgctatg
     9361 acggcatcat cctcgatccg cccaaattcg gccgcggccc ggccggcgag gtgtggcggc
     9421 tcgaggagaa tctgcccgcc ctggtcgccg attgcgcgcg gttgctcgac gcggacagcc
     9481 gcttcctggt gctcaccgtc tatgcggtgc gcatgtccgc gctcgcgatc ggcgagctgc
     9541 tgagccaggc gctggcgggg ctcggcggca gggtcgagtg cggcgacatg gcggtccgag
     9601 aggaaacgcg cggactgctg ctgccgacgg cgatcttcgc ccgctggtcg aaggactagt
     9661 cccgcgcggc gcggatcgcg gcgccggcgg cgaagggagc ggccgcgacc agcagcaaag
     9721 atgccgccgc gagcagctgg agggcgccgg tgccgccgtt cccgccgacg aggccggcgc
     9781 cgaagatcag gatgggtacg gcgagcggca gcagcagaag cccagccagc gcgccggtcc
     9841 ccttgaggcc ggcggtgaga gccgcgaccg tgagggtgag ggcggcgaga ccgggcgtac
     9901 cgaccagcaa gccgagctcc acccgcgcca gcgtctcggc ggacagcttc atcagcgcgg
     9961 ccgcgggcac agcggcgagc atcaacggcg gcccgaagct cagccaatga ccggcaagct
    10021 tggcgagcgc gaccgcttcc tcgctgaagc cgcgcagcgc gagctggtcg agcacgccgg
    10081 cggcccggtc cggctcgacc agccgctcaa cggggagcag agcggcgagc agggccgcca
    10141 tccacagcgc cccgccgccg gccttgacga gcaaaggccg atccgggccg acggcgaacg
    10201 ggaaaagagc ggccacgagc aggaagaagg ccagcggcag catcgcattg ccgccgctgg
    10261 ccgcgatccg aagctcgcgc aggaccagcg caatcatccg agcgcgacct cgtcggcttg
    10321 atccagcccg agcggctggt gcgtggcggc gagcaccgcc ccgcccgccg cccggtgctc
    10381 ggcgatcgcc cgcgccagcc gggcctgccc gtccgcgtcg aggccgttca acggttcgtc
    10441 gagcagccac agccgggcgg ggccggcgag cacgcgcgcc agcatcgccc gcttgcgctg
    10501 tccggtggag agcatccgca ccggcacccg cgccagctcg gcgaggccca ttgcggccat
    10561 cgccggcccg acctccacgc gatcgaggcc cgcccagaaa gcgagcgccc gctccagcgt
    10621 cagccgctcg tcgagcgcgg cgcggttgtc ggccagcgcc gcggtagcgc gctcgatacg
    10681 gccggcggcg gcatcgagca gtccggcgac gaggcggagc aggctcgact tgccggcgcc
    10741 gttcggcccg gtgacgacgg ccgcgccgcc cgcctgcagc ctgaggttca ggccctcgaa
    10801 cagcagccgg ccgccgcgcc ggcaggcgac gtcttccagc accagcagcg gcggaccgct
    10861 cactcgccgt cgccctcggc cgcttcctcg atggcgtgca tgtcctcgtc ggagaagccg
    10921 aaatggtggc cgacctcgtg gacgatgaca tgggtgatga gatcttcgag gctgacgccg
    10981 tcttcggccc attcgtcgag cagcggccgg cggtagaggt ggatcatggc gggcagctcg
    11041 cccgacgcca tcgacgagcg ctcgccgatc ggccggccgg tatagaggcc ggtaagatcg
    11101 aacggatcct ctatgccgag ctcggccagc accgcctcct cggcaaaatc gtcgatccgc
    11161 aggacgacgc cgtcgaggtg gcggcggaag ggctcgggca ggcgcgcgat cgccgcttcg
    11221 gcgaggcgct cgatctcggc ggcgtcgggc gcgaaggttt gacggtcgct caccggcagc
    11281 gacataaggc cgagacgaag attgcgccag cgcgggagga tcggacggtg aggacattga
    11341 gcgaggaaga ggcggccgag gccctggccg ggctgcccgg ctggagcttc gatgcggggc
    11401 ggggcgcgat cctcaagagc ttccgcttcg ccggtttcgc ggaggcgttc gggttcatga
    11461 cggcggtggc gatcgccgcc gagaaggccg atcaccaccc ggactggagc aatagctgga
    11521 accgggtcga catcctgctc accacccata gcgccggcgg cctcaccgcc cgcgacctcg
    11581 atctcgccgg ccgcatcgaa gcgctcgccg ggtcgcttgc cctcgaggcc cccggcgatt
    11641 agagggcgct tcttccagag catgcggagc ggtggccgag tggtcgaagg cgctcgcctg
    11701 gaaagtgagt atacgccaaa agcgtatcga gggttcgaat ccctcccgct ccgccacctt
    11761 atcgaccaaa gaacttcaaa gtagcgtcag aagcctagct ttttcaggct ttccggggtg
    11821 ccgagcgtcc gacgctgtcc gggggtacgc ggtacaatcc cgccgcttgt tgggggcatc
    11881 gtagagacac tctcgagtcg aacgggggca tcatgttgac cgacatgaag gcgcgtcaag
    11941 ctaggccgaa ggagaaggat tacaagctag gggacgccgg cgggctctac ctcttcgtga
    12001 cgaaaaaggg gttcaagtcc tgcgcatgaa gtatcggtac gccggcaagg agaagcgact
    12061 tgtgttcgag ccatactcgg atgtctcgct agccgaggcg cgcgagcgtc gtgatgatgc
    12121 gaagcgccag cttcgggatc tgcgcgatcc ggctgtcgaa gttatgaaac gccgcctcac
    12181 ggccgcggcg gatcagctag ccactttcga gaacgtagcc cgccgatggc acgctatgca
    12241 tgaacctcgg tgggcgaccg tacactcgca cgatgttcta cgaagcctag agcgggaagt
    12301 gtttccctcg ctgggcccga tcccagtccg agagatagac gcaccgctcg tgctggctgc
    12361 cctgcgaagg attgaagctc gagg
//
LOCUS       DASVRK010000082         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_387145, whole genome shotgun sequence.
ACCESSION   DASVRK010000082 DASVRK010000000
VERSION     DASVRK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387145"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..761)
                     /locus_tag="VF589_02835"
     CDS             complement(42..761)
                     /locus_tag="VF589_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8256543.1"
                     /translation="MFGALIRRKIGSGRAQRDELAFESATFSLTTDVPRPTDRRADDR
                     IAPILPLARLIAEDWQDLCRIRNISAGGLMAETAGTPPASGARLSIELDSSHRLPGQV
                     VWVRDAMFGIKFDETVDVRELLAGHRPRGGFAPRPPRLEVTCGATVKIGALYHKVEVR
                     DISQGGMRVALSDWQSVGKPVVATVESLRPVKGVIRWYREGHAGIVLDKPLSFEELAE
                     WLGKRVDVASLKTGAWDKGRR"
     gene            1030..>2207
                     /locus_tag="VF589_02840"
     CDS             1030..>2207
                     /locus_tag="VF589_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8256544.1"
                     /translation="MSDLFSSAFPSADYDASSIEVLEGLEPVRRRPGMYIGGTDERAL
                     HHLAAEVLDNAMDEAVAGFANRIEVTLEPGNRLTITDNGRGIPIDEHPKYPGKHALEV
                     ILTMLHSGGKFSHKAYATSGGLHGVGVSVVNALSVETVVEVARAKQLYRQTFSRGLPT
                     SPLEALGPTQNRRGTALTFTPDLEIFGEHAKFSPARLYRLARSKAYLFAGVEIRWRCD
                     PALIDDDTPAEAVFQFPGGLSDHLKEQVGGRECATAEFFSGRQDFSNGQGSVEWAVAW
                     PLWSEGGSSYYCNTIPTPDGGTHEQGMRAALTRGLRAFGELVGQRKAKDIQSEDAVSG
                     AEIMLSVFIRDPQFQSQTKDRLTSPDAARLVEAAVRDHFDHFLADHMDRGRALLGFVL
                     "
BASE COUNT          347 a          793 c          718 g          349 t
ORIGIN      
        1 acccgcaaga ttccagcttg cgctggaatg acgattcctc ctcagcgccg gcccttgtcc
       61 cacgccccgg tcttgaggct ggcgacgtcg acccgcttgc cgagccattc ggcgagctcc
      121 tcgaagctca gcggcttgtc gaggacgatg ccggcatggc cctcgcggta ccagcggatg
      181 acgcccttca ccgggcgcag gctctcgacc gtggcgacga ccggcttccc cactgactgc
      241 cagtcgctca gcgccacccg catgccgccc tgcgaaatgt cgcgcacctc gaccttgtgg
      301 tagagcgccc cgatcttgac ggtggcgccg caggtgactt cgagccgcgg cgggcgcggg
      361 gcgaagccgc cgcgcgggcg gtggccggcg agcagctcgc gcacgtcgac cgtctcgtcg
      421 aacttgatac cgaacatggc gtcgcgcacc cacaccacct ggccgggcag gcggtggctg
      481 gaatcgagct cgatggagag gcgcgcgccg ctcgccggcg gggtgccggc ggtttccgcc
      541 atcagcccgc cggccgagat gttgcggatc cggcaaaggt cctgccagtc ctcggcgatc
      601 agcctggcga gcggcaggat cggcgcgatg cggtcgtcgg cgcggcggtc ggtcgggcgc
      661 ggcacgtcgg tggtgagcga gaaggttgcg ctttcgaagg cgagctcgtc gcgctgggcg
      721 cggccgctgc cgatcttgcg gcggatcaat gctccgaaca tggcgtgcct ctctccaaca
      781 gcggaaatta cggccgcaga ggtttaccaa aggtaaaggg gtcgcggcag cggaggccgt
      841 cattccagcg caagcgcctc gtcattccag cgaaagctgg aatctccctt cttcgtcccc
      901 gcccgcaaga ttccagcttg cgctggaatg acgggagggg ccccggaatg acggatctcc
      961 cccacgcacc ccgccgcccc cacccccgca cccgcttggc atcggggggc ggtgcgctat
     1021 agctgatcca tgtcagattt gttctcgtcc gctttcccct ccgccgatta cgacgcctcc
     1081 tcgatcgaag tgctcgaagg gctggagccg gtccggcggc ggccgggcat gtatatcggc
     1141 ggcaccgacg agcgggcgct ccaccacctc gccgccgaag tgctcgacaa tgcgatggac
     1201 gaggccgtcg ccggcttcgc caaccgcatc gaagtgacgc tcgagcccgg caaccggctc
     1261 accatcaccg acaatggccg cggcatcccg atcgacgagc accccaaata tccgggcaag
     1321 cacgcgctcg aagtcattct gacgatgctc cattcgggcg gaaaattctc gcacaaggcc
     1381 tatgccacgt cgggcgggct gcacggcgtc ggcgtcagcg tcgtcaacgc gctatcggtc
     1441 gagacggtgg tggaggtggc gcgcgccaag cagctctacc gccagacctt ctcgcgcggc
     1501 ctccccacct cgccgctgga ggcgctcggc cccacccaga accggcgcgg caccgccctc
     1561 accttcaccc ccgacctcga aatcttcggc gagcatgcca aattcagccc ggcgcggctc
     1621 taccggctcg cccgctccaa ggcctatctg ttcgccggcg tcgagatacg ctggcgctgc
     1681 gaccccgcgc tgatcgacga cgacacgccg gccgaggcgg tgttccagtt tcccggcggc
     1741 ctttccgacc atctcaagga gcaggtcggc ggccgcgaat gcgccaccgc cgagttcttc
     1801 agtggccgcc aggatttctc gaacggccag ggctccgtcg aatgggcggt tgcctggccg
     1861 ctgtggagcg aaggcggctc ctcttattat tgcaacacca ttccgacccc cgacggcgga
     1921 acccacgagc agggaatgcg cgccgccctc acccgcgggc tgcgcgcgtt cggcgagctg
     1981 gtcggccagc gcaaggccaa ggatatccag agcgaggatg ccgtgtcggg cgccgagatc
     2041 atgctgtcgg tgttcatccg cgacccccag ttccagagcc agaccaagga ccggctgacc
     2101 agccccgacg ccgcccgttt ggtcgaggcg gcggtgcgcg accatttcga ccatttcctc
     2161 gccgaccata tggaccgcgg gcgggcgctg ctcggcttcg tgctcga
//
LOCUS       DASVRK010000083        13049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_512010, whole genome shotgun sequence.
ACCESSION   DASVRK010000083 DASVRK010000000
VERSION     DASVRK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512010"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VF589_02845"
     CDS             complement(<1..855)
                     /locus_tag="VF589_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8256545.1"
                     /translation="MHIRPISALALASALATPAFAADDAAAVAGQDDGTIIVIGVRKG
                     YAIEATSTATRTPTALNDIPQAITVVTEAQIDDQAMRSIGDVLRYVPGANFGQGEGHR
                     DQIVLRGNNSTADFFSDGLRDDVQYYRGLYNVERVEVLKGPNAMIFGRGGGGGIVNRV
                     TKTPLANGFVRGSAGVDSEGGFLLEADLNQPLAASAAVRLNAAYERFDTHREVYEGER
                     FAINPTAAVTLGEATRIDLSYEYNKDDRVVDRGVPSFEGRPLTGFRDTFFGDPEVNDT
                     DFDAHILKA"
     gene            complement(1067..4507)
                     /gene="smc"
                     /locus_tag="VF589_02850"
     CDS             complement(1067..4507)
                     /gene="smc"
                     /locus_tag="VF589_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX8256546.1"
                     /translation="MQIKRIKLSGFKSFVEPAELRIEPGLTGVVGPNGCGKSNVLEAI
                     RWVMGESSPKSMRGAGMEDVIFAGTAARPARDFAEVSLLVERKAEDVPGDTQFARDGE
                     VEVTRRIERGAGSAYRVNGRDVRARDVALLFADAATGAHSPALVSQGKIAQVIAAKPT
                     ERRMMLEEAAGIAGLHVRRKDAEQKLRAAEANLSRLDELLAEMEQRAAALRRQAKAAE
                     RYRKLTEQIRLAEGRLIFARWREAAAAADVAKAEAQTAVAAVDRAAEAQRAAAASQSE
                     AAQALAGRRTASQAAREQASTLGHRLAALRAERDTVERRIGELTARRATLAADQAREA
                     ALGEDARAALARLEAEAADIAARLAAAEAARGTIDIRSVEHEDAARAVESALGAARAE
                     QAGEQAEARVARTALDTARARLARAEAEAARLAGQMAEIGDEAPLVETKRAARGKRER
                     AEAALIEATAAVGAAEARREAAAAARDRAESEGASARAALAALESEAKALLKAVASSG
                     GDRALNHVRAEPGFERALAAALGDDLDAVVEGDGARRWVGAEAAADDPPLSAAAVRLA
                     DHVQAPPALARRLAQIGVVENDDGGIRLAVGQRLVTRDGRLRRWDGFVATGIGAAAAE
                     RLIRVNRLAEIEQALPTAAAAVEGAQGAIVAAYAEMQGAREAAERGRRAEAEANGAIR
                     DAGREEDAANVAIERLDVRRTGLAERMAQAEADLAEAKAGAAEAEAMVAALPDAAATE
                     ARVSELRKTAEAAQAALAEVRAEAATYARAVSADKQRAEAAKKEHEDWRKRAAEAGRR
                     QADMARRIAEAEEEAERLADAPAALAAQLDTIETEATAAAEAAAAAAAYERAAEETLR
                     AVEKLYADAGEALSIAREARAGALARAENQESRRIEMGRIAGERFECPPPLLPERLGF
                     DAATIAPAQAESAGLDRATQDRERIGPVNLVAERELSELEQGRMHSQAEREELGLAIN
                     RLRGSIGSLNREGRARLLAAFESVDRHFRSLFTTLFSGGQAHLELVESDDPLEAGLEI
                     MAQPPGKRLSALTLLSGGEQALTAIALIFALFLTNPAPICVLDEVDAPLDDANIERFC
                     DLLDRMTGETDTRYLIVTHNAVTMSRMHRLFGVTMIERGISRLVSVDLGGAERLLAAE
                     "
     gene            complement(4538..5239)
                     /locus_tag="VF589_02855"
     CDS             complement(4538..5239)
                     /locus_tag="VF589_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8256547.1"
                     /translation="MKRFLLPAVAAAALVPLGMVPAPGLGAQAQRNWTNVVAETPEGG
                     FRLGNPAAPIKLVEYGSFTCNHCARFAGEGVPQLIGKYVKSGRVSFEYRTFVRDPADF
                     SAALLARCAGPAAFFPLSHDYFASQSQWIGRYQAMTDEQKKAIDALPMERKLGRFAAV
                     GGLNAIAAKAGVPAAKAAICLADADAMRKLIEMRRVASETHKVEGTPSFLINGKLVEA
                     HDWGALEPLLAGPGG"
     gene            complement(5241..5990)
                     /locus_tag="VF589_02860"
     CDS             complement(5241..5990)
                     /locus_tag="VF589_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8256548.1"
                     /translation="MKAIFSTAAAALALALAGCGGESGAGNAGAAGGKSMAAIPAPNG
                     GDWTQTFAQTEAGGFLMGNPNAPVKIIEFASMTCPHCAEFAETGLPPLTEKYVRTGLA
                     SLELRNFVRDSPDMAASLLARCNGPAAFFKLSDQMFGAQSEWFGKLQAMSPADQQRLQ
                     TLPPQQAVGVVAEQAGLVDFVRVRGIPETKARQCLSNQAELNRLVQMAQQATTDFPEL
                     PGTPSFVINGQLAKNAGTWATLEPQIQAAMQ"
     gene            complement(5993..6661)
                     /locus_tag="VF589_02865"
     CDS             complement(5993..6661)
                     /locus_tag="VF589_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX8256549.1"
                     /translation="MPRYAMLRAMGKSGGTDKERPEPQRAKRAVAAAAPERTRRARSV
                     GDVVPERARRARSVGDMVPDVGRAAFRRFGFVQSSVVSRWREIVGERYAGVSAPESIR
                     FPPGKRSRGVLNLIVEGAHAPMMQHVAPVIIERVNRFFGYPAVERVAFRQGLVKVAAA
                     KARAAPPSLRPLSAELGDSLRAIADPELRAVLESLAQGVAASQGRPVPAEMMTIPVVG
                     KLKG"
     gene            6660..7694
                     /locus_tag="VF589_02870"
     CDS             6660..7694
                     /locus_tag="VF589_02870"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX8256550.1"
                     /translation="MAVDASHALLAWYAVDKRALPWRAEAGETPDPYRVWLSEVMLQQ
                     TTVAHARPYFEAFSRRWPDVAALAATDEGELMAAWAGLGYYARARNLIACARMVAKRH
                     GGRFPDSEAELGKLPGIGRYTAAAVAAIAFGRRAVVVDANVERVVARLFAVGTPLPAA
                     RAAIYALADGITPDDRAGDFAQAIMDLGATVCTPRRPACGRCPLAPSCRARREGNPEL
                     YPVKAAKASKPQREGNAYWLEHDGHVLLVRRPPRGLLGGMLALPTGAWGEAAAPGDGA
                     PAAARWSDAGTVDHVFTHFALRLRLFCARAAGREADQLWWPVDRLAEAGLPTLFAKAA
                     ARGAEWRRAA"
     gene            7691..8545
                     /gene="nudC"
                     /locus_tag="VF589_02875"
     CDS             7691..8545
                     /gene="nudC"
                     /locus_tag="VF589_02875"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX8256551.1"
                     /translation="MRPGFTGSTVDRADHLRLDLQRLAELRAHASARLLRLAVHDPCL
                     EDGRLAWGTLADSSAEEDLIFLGLDGDIPLFAPLPRVEPGQRAWAVFGLLQDMASEDA
                     ALWGTARSLIEWHNRNGFCSNCGTPTRLFRAGWGRKCDTCQAEHFPRVDPVVIMLAQH
                     DGRVLVGRQPQYPARRYSALAGFVEPGESIEEAVARELKEEAGIAVANVRYVASQPWP
                     FPGSLMIACIADAASDALTLDRNELEDAFWVDRAGVAAALAGEAGAPFLAPPHFAIAH
                     TLFARWLA"
     gene            8650..9363
                     /locus_tag="VF589_02880"
     CDS             8650..9363
                     /locus_tag="VF589_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX8256552.1"
                     /translation="MDDRFNTIAGWVLGAGIVALGAALVTGEVFKSERPEKMGYPIEG
                     VEEEGEGGSDAEQPIAFFMASADPRLGEQVFKKCAACHTATPGGPNGLGPNVHGIMGQ
                     PVAARPGFAYSDALKGKGGTWDWEAMSQWLKSPKSFAPGTKMTFAGLSKPEDRANLMA
                     WLNTQGSNLPLPPPPAAAGNPAQAKAEAADMPALGDKGEDEPVLTEGQAAKQPGGRVM
                     GEGAPSTAGRDEQSKAKGE"
     gene            complement(9377..12346)
                     /locus_tag="VF589_02885"
     CDS             complement(9377..12346)
                     /locus_tag="VF589_02885"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8256553.1"
                     /translation="MLADRFAAPNAEGMSEDYAARCGCAACRGSDSALEAPVISAAAA
                     ADEPDGTIAVGGSASGEIDTAGDVDSYSFNVVAGQTYTVSLRGTGATPLPDTFLTLFN
                     NVGVEVDSNDDGGTGTNSLLTFTATTTGSWEIQAEGYPGDGHTGGYTVDLFQHGADSV
                     AGDTTTTDAIPIGGTIFGAIESDGDVDVYSVSLTAGSLYVFEVAGGADYESNAPAVPP
                     GQLDTIISILDAEGNVLATNDDILFPSDPSSAASFVAEEGGVYFVQVEAYDGQTGGYA
                     LKAEEHSIAGSPLETIDWGTKVGTNAITVYFAETGEIFDGVASLGWTAYEKGRAVAAF
                     QEYADVANLTFSVVDDPALATFKLVTDASADYLGYFNPPGETNEGVGVFSTAGKGWDR
                     TGATGGLEKGGYGFITLIHEIGHGLGLAHPHDDGGTSTTMPGVSGPFGSYGPHDLNQG
                     VYTTMSYNDGWVRHPNGVPESDAYGWQSGPMALDIAVIHEKYGAAARNAGNTTYILPS
                     ANAAGTYWSSIWDTGGNDTIVHDGPAAATIDLRPATLDYSPTGGGAISYVDGVFGGFT
                     IANKVLIENASGGAGADRLIGNVAANRLDGRGGADRMEGGLGNDRYTVNHALDAVIEA
                     ANAGGADRILAKVSYGLALGVHVEVLSAANAGGTTAIRLTGNSYDNNLIGNAGRNVLN
                     GRVGADTLTGLGGNDIFRVDNVGDRVVEADNGGRDRVIAYVSFALGEDSEVELLGAAN
                     PAGTTAINLSGSDARNVITGNAGANLLDGGDGVDTLKGLGGNDRFRVDHAGDRIVEAA
                     AGGTDRVFATVSYALDAGAHVETLSTASDAGTGAINLTGNALSNQIIGNAGANLLRGL
                     GAGDTLRGLGGADRLLGGGGRDDLIGGAGADRFYFDAPLSAATNVDDILDFATEDSIF
                     LDRSVFTGIPKNGFLAASAFNTGTAAQDASDRILYDQASGRIFYDADGVGGTAAILFA
                     KLDPGTALTSADFVAVA"
     gene            complement(12453..>13049)
                     /locus_tag="VF589_02890"
     CDS             complement(12453..>13049)
                     /locus_tag="VF589_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256554.1"
                     /translation="DIVRETAGEGRDALTSSVSYVLARGSRVELLSTNNEAATDAIAF
                     TGNEIANTIVGNAGINLLRGEGRGDSLSGLGGGDRLFGGAGRDALTGGGGADRFYFDS
                     GLNASTNVDRILDFTAADTIVLDRTIFSGIAADGALAPGAFRNGVAAHDSGDRIVYDK
                     ATGQIFYDADGAGGAAAVLFAKVDAGTLLTSEDFIAVA"
BASE COUNT         1750 a         4629 c         4595 g         2075 t
ORIGIN      
        1 cgccttcagg atgtgggcgt cgaaatcggt gtcgttgacc tcggggtcgc cgaaaaaggt
       61 gtcgcggaag ccggtgagcg gccgtccctc gaaggacgga acgccgcggt ccaccacccg
      121 atcgtccttg ttatattcgt aggaaaggtc gatccgcgtc gcctcgccca gcgtgacggc
      181 ggcggtcggg ttgatcgcga agcgctcgcc ttcatagacc tcgcggtggg tgtcgaaccg
      241 ctcataggcc gcgttgagcc gcaccgcggc actggcggcg agcggctggt tgaggtcggc
      301 ctccaggagg aagccgcctt cgctgtcgac gccggcgctg ccgcgcacga agccgttggc
      361 gagcggcgtc ttggtgacgc ggttgacgat gccgccgccg ccgccgcgtc cgaagatcat
      421 ggcgttcggc cccttcagca cctcgacccg ttcgacattg tagaggccgc gataatattg
      481 gacgtcgtcg cgcagcccgt cgctgaagaa gtcggccgtg ctgttgttgc cgcgcagcac
      541 gatctggtca cggtggccct cgccctggcc gaaattggcg ccggggacgt agcgcagcac
      601 gtcgccgatc gagcgcatcg cctggtcgtc gatctgcgct tcggtgacca ccgtgatcgc
      661 ctgcgggatg tcgttgagcg ccgtcggcgt gcgggtggcg gtgctggtcg cctcgatcgc
      721 atagcccttg cgcacgccga tgacgatgat cgtgccgtcg tcctgcccgg caaccgcggc
      781 ggcgtcgtcg gcggcgaaag cgggggtggc gagcgccgag gcgagcgcca gggcggagat
      841 cggacggatg tgcatgtggg ccccttttcg gacgagcgca tgtttgcgtc tcgcggcagc
      901 cgtctaatgc tgttgcgatt tattcgcaat agcattcgga gtctggacaa atctccggcg
      961 cgcgctcggc cggatctacg cggcgacccg cttgatctcg ccttgcgtgg cgacccactg
     1021 gttcgtcatc ttcgactgaa gattgtgcgc ggcggcccgt tctgattcat tcggccgcga
     1081 gcagccgttc cgcgccgccg agatcgaccg acaccagccg gctgatgccg cgctcgatca
     1141 tcgtgacacc gaacaggcgg tgcatgcggc tcatcgtcac tgcattgtgg gtgacgatca
     1201 gatagcgtgt gtcggtctcg ccggtcatcc ggtccaggag gtcgcagaag cgctcgatat
     1261 tggcgtcgtc cagcggcgcg tcgacctcgt cgaggacgca gatcggcgcg gggttggtga
     1321 ggaacaatgc gaagatgagc gcgatcgcgg tcagcgcctg ctcgccgccc gacagcaggg
     1381 tcagcgcgga cagtcttttg cccggcggtt gagccatgat ctccagcccc gcctcgagcg
     1441 ggtcgtccga ttcgaccagt tcgagatgcg cctggccgcc gctgaacagg gtcgtgaaca
     1501 ggctgcggaa atggcggtcg accgattcga acgccgccag cagccgcgcg cggccctcgc
     1561 gattgaggct tccgatcgat ccgcgcagcc ggttgatcgc cagccccagt tcctcgcgct
     1621 cggcctgcga atgcatgcgg ccttgctcga gctcggaaag ctcgcgctcg gcgaccaggt
     1681 tgacggggcc gatccgctcg cggtcctggg tggcgcggtc gagccccgcc gactcggcct
     1741 gggcaggggc gatggtggcg gcgtcgaagc cgagccgttc gggcagcaaa ggcggcgggc
     1801 attcgaagcg ctcgccggcg atgcggccca tctcgatgcg gcggctttcc tgattctcgg
     1861 cccgggcgag ggcgccggcg cgcgcttcgc gggcgatcga cagcgcctcg ccggcatcgg
     1921 cataaagctt ttcgacggcg cgcagggtct cctcggcggc gcgctcataa gcggcggcgg
     1981 cggcggcggc ttcggcggcg gcggttgctt cggtttcgat cgtgtcgagc tgggcggcga
     2041 gcgccgccgg cgcatcggcc agccgctccg cctcttcctc ggcctccgcg atgcggcgcg
     2101 ccatgtcggc ctgacgccgg ccggcttcgg cggcgcgctt gcgccaatcc tcatgttcct
     2161 ttttggccgc ctcggcgcgc tgcttgtcgg cgctcacggc gcgggcgtac gtcgcggctt
     2221 cggcgcgcac ttcggcgagc gcggcctgcg cggcttccgc cgtcttgcgc aactcggaca
     2281 cccgagcttc ggtcgcggcg gcgtcgggga gggccgcgac catcgcctcg gcctcggcgg
     2341 cgccggcctt cgcttcggcc aggtcggcct ccgcctgcgc catacgctcg gcgaggccgg
     2401 tgcggcggac gtcgagccgc tcgatcgcga cattggcggc atcctcttcg cggccggcgt
     2461 cgcggatcgc gccattggcc tcggcttccg cccgccgccc gcgctcggcc gcctcgcgcg
     2521 cgccctgcat ctcggcataa gcggcgacga tcgcgccttg ggcgccttcg accgcggcgg
     2581 cggcggtggg gagcgcctgc tcgatctcgg cgagccggtt gacccggatc agccgctcgg
     2641 ccgcggcggc gccgatgccg gtcgcgacga agccgtccca gcggcgcagc ctcccgtcgc
     2701 gggtgacgag gcgctgcccg accgccagcc ggatgccgcc gtcgtcgttc tcgacgaccc
     2761 cgatctgcgc cagccgccgc gccagcgccg gcggcgcctg gacatggtcg gcgagcctga
     2821 ccgccgccgc gctcagcggc gggtcgtccg ccgcggcttc ggcgccgacc cagcggcgcg
     2881 ccccgtcgcc ttcgacgacg gcgtcgagat cgtcgccgag cgcggcggca agcgcgcgct
     2941 cgaagccggg ctcggccctg acatgattga gcgcgcggtc gccgccgctg ctcgccaccg
     3001 ccttcagcaa cgccttggct tcggattcga gcgcggccag cgcggcgcgg gcggaggcgc
     3061 cctcgctttc ggcacgatcg cgcgcggccg cggccgcctc gcgccgggct tcggccgctc
     3121 cgaccgcggc ggtggcttcg atgagcgcgg cttcggcgcg ttcgcgcttg ccgcgggcgg
     3181 cgcgcttagt ctcgaccagc ggcgcctcgt cgccaatctc cgccatctgg ccggccagcc
     3241 gcgccgcttc ggcctcggcc cgggcgagcc gcgcgcgcgc cgtgtccagc gctgtcctgg
     3301 cgacccgcgc ctcggcctgc tcgccggcct gctcggcccg cgccgccccg agcgcgcttt
     3361 cgaccgcccg ggcggcatcc tcatgctcga cgctgcgaat gtcgatcgtg ccgcgcgccg
     3421 cttccgccgc cgccagccgc gcggcgatgt cggccgcctc ggcctcgagc cgggccagcg
     3481 ccgcgcgcgc atcctcgccc agcgccgcct cgcgcgcctg gtcggcggcc agcgtcgccc
     3541 gccgcgccgt caattcgccg atgcgccgtt cgaccgtgtc gcgctcggcc cggagcgccg
     3601 ccagccgatg cccgagcgtt gacgcctgct cccgcgccgc ctgcgaggcc gtgcgccggc
     3661 cggccagcgc ctgcgcggct tcgctttggc tggcggcggc ggcacgctgc gcctcggccg
     3721 cccggtcgac cgcggcaacg gcggtctggg cttcggcctt ggcgacgtcg gcggcggcgg
     3781 cggcctcgcg ccagcgcgcg aagatcagcc ggccctcggc aagccggatc tgctcggtca
     3841 gcttgcggta acgttcggcg gccttggcct ggcggcgcag cgccgcggcc cgctgctcca
     3901 tctcggcgag caattcgtcg agccggctga gattggcctc ggcggcgcgc agcttctgct
     3961 cggcatcctt gcggcggacg tggagcccgg cgatgccggc cgcttcctcc agcatcatcc
     4021 gccgctcggt cggcttggcc gcgatcacct gcgcgatctt gccctggctg accaaagccg
     4081 gcgaatgcgc gccggtcgcc gcgtccgcaa acagcaaagc cacgtccctc gcccggacgt
     4141 cgcgcccgtt cacccgatag gcggagccgg cgccgcgctc gatccggcgg gtgacctcga
     4201 cctcgccgtc gcgggcgaat tgggtgtcgc ccggcacatc ctccgccttg cgctcgacca
     4261 gcagcgagac ttcggcgaaa tcgcgggcgg ggcgggcggc cgtgccggcg aagatcacat
     4321 cctccatgcc ggcgccgcgc atcgacttgg ggctcgattc gcccatcacc caacggatcg
     4381 cttcgaggac gttggatttg ccgcagccat tggggccgac gacgccggta aggccgggct
     4441 cgatgcgcag ttcggcaggc tcgacaaagc ttttgaagcc cgaaagcttg atgcgcttta
     4501 tctgcacgtc agccccccgg cccccatctt tgcgtcctta tcctcccggg ccggcgagca
     4561 aaggctcgag cgcgccccag tcatgggcct cgacgagctt gccgttaatg aggaagctgg
     4621 gcgtcccctc caccttgtgg gtctcggacg ccacccggcg catctcgatc agcttgcgca
     4681 tggcgtcggc atcggccagg catatcgcgg ccttggcggc gggaacgccg gccttggcgg
     4741 cgatcgcgtt taggccgccc acggccgcga atcgtcccag cttgcgctcc atcggaagcg
     4801 cgtcgatcgc cttcttctgc tcgtcggtca tcgcctggta gcggccgatc cactggctct
     4861 ggctcgcgaa ataatcgtgg ctcagcggga agaaggcggc cggcccggcg cagcgggcga
     4921 gcaaagccgc cgaaaagtcg gccggatcgc gcacgaaggt gcgatattcg aagctgaccc
     4981 ggccgctctt cacatatttg ccgatcagtt gcggcacgcc ctcgccggcg aaccgcgcgc
     5041 aatggttgca ggtgaaggag ccatattcga cgagcttgat gggcgccgcc gggttgccga
     5101 ggcggaagcc gccttcgggc gtttcggcga cgacattggt ccaattgcgc tgcgcctggg
     5161 cgccgaggcc gggggccggg accatcccca gcgggaccaa agcggcggcg gcgacggcgg
     5221 gaaggaggaa gcgcttcatc ttactgcatc gccgcctgga tctggggctc gagcgtcgcc
     5281 caggtgccgg cattcttcgc cagctggccg ttgatgacga agctcggcgt acccggcagc
     5341 tccgggaagt cggtggtcgc ctgctgggcc atctggacca gccggttgag ctcggcctgg
     5401 ttggagaggc actggcgcgc cttggtttcg gggatgccgc ggacgcggac gaaatcgacg
     5461 aggccggcct gctccgccac cacgcccacc gcctgttgcg gcggcagcgt ctgcagccgc
     5521 tgctggtcgg ccgggctcat cgcctggagc ttgccgaacc actcggactg cgcgccgaac
     5581 atctggtcgc taagcttgaa gaaggcggca ggcccgttgc agcgggcgag cagcgaggcg
     5641 gccatgtccg gggaatcgcg cacgaagttg cgaagctcca ggctcgccag cccggtgcgg
     5701 acatatttct cagtgagcgg cggcagcccg gtctccgcga actcggcgca atgcgggcag
     5761 gtcatcgacg cgaattcgat gatcttcacc ggcgcgttcg ggttgcccat caggaagccg
     5821 ccggcttcgg tctgcgcgaa cgtctgggtc cagtcgccgc cattgggcgc ggggatcgcc
     5881 gccatcgact tgccgccggc cgctcccgca ttgcctgcgc cgctttcgcc gccgcagccg
     5941 gcgagggcca gcgcgagcgc cgccgcggcc gtggagaata tcgccttcat ccttaccctt
     6001 tcaatttgcc gaccacgggg atcgtcatca tctcggccgg aaccgggcgc ccctgactcg
     6061 cggcgacccc ttgcgcaagc gattccagca ccgcccgcaa ttccgggtcg gcgatcgcgc
     6121 gcaggctgtc gccaagctcg gccgagagcg gccggagcga gggcggcgcg gcacgcgcct
     6181 tggcggcggc gaccttaacc aggccttgcc tgaaggcgac gcgctcgacc gccggatagc
     6241 cgaagaagcg gttgacgcgc tcgatgatga ccggcgcgac atgctgcatc atcggcgcat
     6301 gggcgccttc gacgatcagg ttcagcacgc cccggctgcg cttgccgggc gggaagcgga
     6361 tcgattccgg ggccgacaca ccggcataac gctcgccgac aatctcccgc cagcggctca
     6421 ccaccgagga ttggacgaag ccgaagcggc ggaaggcggc gcgcccgacg tcgggcacca
     6481 tgtcgccgac ggagcgggca cggcgtgccc gttccggaac gacatcgccg acggagcggg
     6541 cacggcgcgt ccgttccggc gcggcagcgg ccacggcgcg cttcgcccgc tgcggctccg
     6601 gccgctcttt gtcggtaccg cccgatttgc ccatcgcgcg cagcatggca tagcggggca
     6661 tggccgtcga tgcttctcat gccctgctcg cctggtatgc tgtggataaa agggcgctgc
     6721 cgtggcgcgc ggaagcgggc gagacgcccg atccctatcg cgtctggctg tccgaagtga
     6781 tgctccagca aaccacggtc gcccatgcgc ggccctattt cgaagccttc agccgccgct
     6841 ggcctgacgt ggcggcgctg gccgcgaccg acgagggcga gctgatggcg gcctgggcgg
     6901 ggctcggtta ctatgcccgc gcccgcaacc tgatcgcctg cgccaggatg gtggccaagc
     6961 gccatggcgg ccgctttccc gacagcgagg cggaactcgg caagctcccc ggcatcggcc
     7021 gctataccgc ggcggcggtg gcggcgatcg ccttcggccg gcgcgcggtc gtcgtcgacg
     7081 ccaatgtcga gcgggtggtg gcgcggctgt tcgcggtcgg gacgccgttg ccggccgcgc
     7141 gcgcggcgat ctacgcgctc gccgatggca tcacgccgga cgatcgggcg ggcgacttcg
     7201 cacaggcgat tatggatctc ggcgccaccg tctgcacgcc gcgccggccc gcctgcggcc
     7261 gctgcccgct cgccccctcc tgccgggccc ggcgcgaggg gaatccggag ctttatccgg
     7321 ttaaggcggc caaggcctcg aagccccagc gcgaaggcaa tgcgtattgg ctggagcatg
     7381 acggccacgt cctgctggtc cggcgccccc cgcgcggcct gctcggcggc atgctcgcgc
     7441 ttccgaccgg cgcatggggc gaggcggctg cgcccggcga cggcgcaccg gccgcggccc
     7501 ggtggagcga cgccggcacg gtcgaccatg tcttcaccca tttcgcgctc cgcctccgcc
     7561 tgttctgcgc ccgcgccgcc ggccgcgaag ccgaccagct ctggtggccg gtggaccgcc
     7621 tcgccgaggc cggcctgccg accctgttcg ccaaggcggc ggcccgcggc gccgaatggc
     7681 ggcgggcggc atgaggcccg gcttcaccgg ctccacggtc gaccgcgccg accaccttcg
     7741 cctcgacctt cagcgtctcg ccgagctccg cgcccacgcc tccgcccggc tgctgcggct
     7801 ggcggtgcac gatccctgcc tcgaggacgg ccggctcgcc tggggcaccc tcgccgatag
     7861 ctcggccgag gaggacctga tcttcctcgg tctcgacggc gatattcccc tgttcgcgcc
     7921 gctgccgcgg gtcgaacccg gccagcgcgc ctgggcggtg ttcggtttgc tccaggacat
     7981 ggcgagcgag gatgcggctt tgtggggcac cgcccgcagc ctcatcgaat ggcacaaccg
     8041 caatggcttc tgctcgaact gcggcacgcc gacgcggctc ttccgggccg gctggggacg
     8101 caagtgcgac acctgccagg ccgagcattt cccgcgcgtc gacccggtgg tgatcatgct
     8161 cgcccagcat gacgggcggg tgctggtcgg ccgccagccg caatatccag cgcggcgcta
     8221 ttcggcgctg gccgggttcg tcgagccggg cgagtcgatc gaggaagcgg tggcgcgcga
     8281 gctcaaggag gaagccggga tcgccgtcgc caacgtccgc tacgtcgcca gccagccctg
     8341 gccctttccc ggctcgctga tgatcgcgtg catcgccgac gccgcctcgg acgcgctgac
     8401 gctcgaccgc aacgagctcg aggacgcctt ctgggtcgac cgcgcgggcg tcgccgcggc
     8461 gcttgccggg gaggccggcg cgcccttcct cgcgccgccc catttcgcca tcgctcatac
     8521 tctgttcgcg cgctggctgg cttagctcag ccgcttgccc ccgccacccg ccgcgcctaa
     8581 aggggcgcca attcaatcgc gcgcccgcaa ttcctcgcgt cggcgcggct tggcggaggt
     8641 tcgggcggca tggacgatcg gttcaacacg attgcgggct gggtgctcgg cgcgggcatc
     8701 gttgcgctcg gcgccgcgct cgtgaccggc gaagtgttca agtccgagcg gcccgaaaag
     8761 atgggctatc cgatcgaagg cgtcgaggag gaaggcgaag gcggcagcga cgccgagcag
     8821 ccgatcgcct tcttcatggc ctcggccgat ccgcggctcg gcgagcaggt gttcaagaaa
     8881 tgcgccgcct gccacaccgc tactccgggc ggccccaacg ggctcggtcc gaacgtccac
     8941 ggcatcatgg gccagccggt cgcggcccgg cccggcttcg cttattcgga cgccttgaag
     9001 ggcaagggcg gcacctggga ctgggaggcg atgagccagt ggctgaagtc gcccaagagc
     9061 ttcgcgcccg gcaccaagat gaccttcgcc ggcctctcca agcccgagga tcgcgccaat
     9121 ctgatggcct ggctcaatac ccagggcagc aacttgccgc tgccgccgcc acccgccgcc
     9181 gccggcaacc cggcccaggc caaggcggaa gccgccgaca tgccggcgct cggcgacaag
     9241 ggcgaggacg agccggtgct gaccgaaggc caggccgcga agcagccggg cggccgcgtg
     9301 atgggcgaag gcgccccctc caccgccggc cgggacgagc agtccaaggc caagggcgag
     9361 taagcgggag cgcccgctag gccaccgcca cgaaatcggc gctggtgagc gcggtccccg
     9421 ggtccagctt cgcgaacaaa attgcggccg tgccgcccac gccgtcggca tcgtagaaga
     9481 tgcgccccga agcctggtcg tagagtatcc ggtcgctcgc gtcctgcgcg gcggtgccgg
     9541 tgttgaaggc cgaggcggcg aggaagccgt tcttggggat gccggtgaag accgaccgat
     9601 cgaggaagat cgaatcttcg gtcgcgaagt cgaggatgtc gtcgacattg gtcgcggcgc
     9661 tgagcggggc gtcgaagtag aagcggtccg ctcccgcgcc cccgatcaga tcgtcacggc
     9721 cgccgccgcc gagcagccgg tcggcgccgc caagcccgcg cagcgtatcg ccggcgccga
     9781 ggccgcgcag caaattggcg ccggcattgc cgatgatctg gttgcttagc gcgttgccgg
     9841 tgaggttgat cgcgccggtg ccggcatcgc tggcggtgct cagcgtctcg acatgggcgc
     9901 ccgcgtccag ggcatagctc accgtcgcga acacccggtc ggtgccgccg gcggcggctt
     9961 cgacgatgcg gtcgcctgca tggtcgacgc ggaaccggtc gttgccgcca agtcccttga
    10021 gcgtgtcgac gccgtcgccg ccgtcgagca ggttggcgcc cgcattgccg gtgatgacgt
    10081 tcctcgcatc gctgccgcta aggttgatcg cggtggtgcc ggccgggttg gcggcgccca
    10141 gcagctccac ttcggaatcc tcgccgagcg cgaagctcac ataagcgatc acccggtcgc
    10201 ggccgccatt gtcggcttcg acgaccctgt ccccaacatt gtcgacgcgg aagatatcgt
    10261 tgccgccaag cccggtaagc gtgtccgcgc cgaccctgcc gttgaggacg ttgcgccccg
    10321 cattcccgat cagattgttg tcgtaggaat tgccggtgag cctgatggcc gtggtgcccc
    10381 ccgcgttggc ggcgctcagc acctcgacat ggacgccgag cgccagcccg tagctgacct
    10441 tggccaggat ccggtccgcg ccaccggcat tggccgcttc gatcaccgcg tccaacgcgt
    10501 ggttgaccgt atagcgatcg ttgccgaggc cgccttccat ccgatccgcg ccgccgcgcc
    10561 cgtcgagccg gttggccgcg acattgccga tcagccggtc cgcgccggcg ccgcccgagg
    10621 cgttctcgat cagcaccttg ttggcgatgg tgaacccgcc gaacacgccg tcgacatagg
    10681 agatggcccc gccgccggtc ggcgaataat cgagcgtggc gggccggagg tcgatcgtcg
    10741 ccgcggccgg gccgtcatgg acgatggtgt cgttgccccc cgtgtcccag atcgagctcc
    10801 agtaagtgcc ggcggcattg gcgctcggga ggatgtaggt ggtgttgccc gcattgcggg
    10861 cggcggcccc gtatttttca tggatgaccg cgatgtcgag cgccatcggg ccagactgcc
    10921 agccataggc gtcgctctcg gggacgccgt tcgggtgccg cacccagccg tcattgtacg
    10981 acatggtcgt atagacgccc tggttgagat catgggggcc gtagcttccg aacgggccgc
    11041 tgacccccgg catcgtggtc gaggtgccgc catcgtcatg cggatgggcg aggccgagcc
    11101 cgtggccgat ctcgtggatg agggtgatga agccgtagcc gcccttttcg agtcccccgg
    11161 tggcgccggt gcggtcccag cccttgccgg cggtgctgaa cacgccgacg ccttcattgg
    11221 tctcgcccgg cgggttgaaa tagccgagat agtcggcgct ggcatcggtc accagcttga
    11281 aggtggcgag cgcgggatcg tccaccaccg agaaggtgag gttcgcgacg tcggcatatt
    11341 cctggaacgc cgccaccgcg cggccctttt cataggcggt ccagccgagc gaggcgacgc
    11401 cgtcgaagat ctcccccgtc tccgcgaaat agacggtgat ggcgttggtg ccgaccttgg
    11461 tcccccagtc gatcgtctcg agcggcgatc cggcgatcga atgctcctcg gccttgagcg
    11521 catagccgcc ggtctgcccg tcataggcct cgacctggac gaagtagacg cctccttcct
    11581 cggcgacgaa gctggccgcg gagctgggat cgctcgggaa caggatatcg tcgttggtgg
    11641 cgaggacgtt gccctcggcg tcgaggatgc tgatgatcgt gtcgagctgc cccggcggga
    11701 cggcgggcgc gttcgattcg taatcggcgc cgccggcgac ttcgaatacg tagaggctgc
    11761 cggcggtcag cgacaccgaa tagacgtcga cgtcgccgtc gctctcgatc gcgccgaaaa
    11821 tggtgccgcc gatcgggatg gcatcggtgg tggtggtgtc gccggcgacg ctgtcggcgc
    11881 cgtgctggaa caggtcgacc gtgtagccgc cggtatggcc gtcgccggga tagccttcgg
    11941 cctggatttc ccagctgccg gtggtggtcg cggtgaaggt cagcagcgaa ttggtgccgg
    12001 tcccgccatc gtcattgctg tcgacctcga cgccgacatt gttgaacagg gtgaggaacg
    12061 tgtccggcag cggcgtcgcg ccggtgccgc gcagcgagac ggtgtaggtc tggccggcca
    12121 caacgttgaa gctgtagctg tcgacatccc ccgctgtgtc gatctcgccg ctggccgagc
    12181 cgccgaccgc gatcgtgccg tcgggctcgt cggccgctgc ggcggccgaa atcaccgggg
    12241 cctcgagcgc gctgtcgctg cccctgcagg ccgcgcatcc gcatcgcgcc gcataatcct
    12301 cggacattcc ctcggcgttc ggcgcagcga atctatcggc gagcatggca atcccccaat
    12361 tgtccgagtc ggcaaggacg cgaagcccgg aaataaatca ccctttcccc acgacagcaa
    12421 ccgccctgcc gccccgccga tgccgggccc gatcacgcca ccgcgatgaa gtcctcgctg
    12481 gtcagcaagg tgccggcatc gaccttcgcg aacagcacgg ccgccgcgcc gcccgcgccg
    12541 tccgcgtcgt agaagatctg tccggtggcc ttgtcgtaaa cgatgcggtc gccggaatcg
    12601 tgcgccgcga cgccgttgcg gaatgcgccc ggcgcgagcg ctccgtcggc ggctatcccg
    12661 gaaaagatcg tccggtcgag cacgatcgtg tcggcggcgg tgaagtcgag aatccgatcg
    12721 acgttggtcg aggcgttgag gccggagtcg aagtagaagc ggtcggcgcc gccgccgccg
    12781 gtcagcgcgt cgcggccggc gcccccgaac agccggtcac cgccgccaag gccgctgagc
    12841 gagtcgccgc ggccttcccc gcgcagcaga ttgatccccg cattgccgac gatcgtgttg
    12901 gcgatctcgt tgccggtgaa ggcgatggcg tcggtggccg cctcgttgtt ggtgctcaac
    12961 agctcgaccc ggctgccgcg ggccagcacg tagctgacgc tcgacgtcag cgcatcgcgc
    13021 ccctcgccgg ctgtctccct gacaatatc
//
LOCUS       DASVRK010000084         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_512020, whole genome shotgun sequence.
ACCESSION   DASVRK010000084 DASVRK010000000
VERSION     DASVRK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512020"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VF589_02895"
     CDS             <1..376
                     /locus_tag="VF589_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8256555.1"
                     /translation="AGLAGAELRQGDMYALPLASGSADTIIIHQVLHYAQHPAAAVAE
                     AARLLAPGGRLLIVDFAPHEREELRVRDAHVRLGFADDAVVKWMAETGLDGRVVGHLE
                     GGALTVTLWTGEQPEARLKVVA"
     gene            373..1308
                     /gene="metF"
                     /locus_tag="VF589_02900"
     CDS             373..1308
                     /gene="metF"
                     /locus_tag="VF589_02900"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX8256556.1"
                     /translation="MSPVAPAFAASGQPLFENVAGDIDVSFEFFPPKTEKMEETLWES
                     VKTLEPLAPRFVSVTYGAGGSTRARTHATVERIARETSLAAAAHLTCVEASRAEIDEV
                     ARDYWAAGIRHIVALRGDPPEQGRSFQPHPDGYANAVELIRGLKAVAPFEISVAAYPE
                     CHPDSPNKAADLDNLWRKIDAGADRAITQFFFSADCFFRFRDTAAAMGIEAEIVPGIL
                     PVSNVAQTRKFAAMCGATIPSWLDRLFEGLDDLPAARQLVAATVAAELCGQLYAGGVR
                     HFHFYTLNRAELAYAICHLLGLRPKEKSNERRTAA"
     gene            1286..2320
                     /locus_tag="VF589_02905"
     CDS             1286..2320
                     /locus_tag="VF589_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX8256557.1"
                     /translation="MNVAQQLKAEAAKRILVKDGAYGTQVQARRLAEADYRGGLDLGR
                     DQKGNNDLLNLTRPALVGAIADSFACAGADILATNSFNANRISQADYGAETLVRDINA
                     AAARIICEVADRHAQKDGRKRWVAGALGPTNKTLSLSPNVNDPGFREIDFDFLSDVYV
                     EQIESLVEGGAEIVLIETVFDTLNAKAAIHAAGRVDRELPIMLSMTITDLSGRNLSGH
                     SIEAFWASVRHARPLSIGLNCSFGAAQLRAHVASLSGIADALIMAYPNAGLPNDLGDY
                     DECPETTAAQVREWCEQGIVNIVGGCCGTTPEHIAAIAEIVSKYPPRPIAAPVRRTML
                     AGIEPMNLAA"
     gene            2389..>3077
                     /locus_tag="VF589_02910"
     CDS             2389..>3077
                     /locus_tag="VF589_02910"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX8256558.1"
                     /translation="MTATAYLQDSPSPQPSPRERGEGAVSSSFVNIGERTNVTGSAQF
                     KKLIMAGDYAAAVEVARQQVENGAQIIDINMDEGLLDAEYAMTTFLKLIAAEPDIARV
                     PFMIDSSKWSVIEAGLKCVSGKPIVNSISMKEGEAEFLAHARKVRDYGAAMVVMAFDE
                     VGQADTQARKVEICERAYKLLVGDGFPPEDIIFDPNIFAVATGIDEHRNYAVDFIEAC
                     REIRARCPHAH"
BASE COUNT          533 a         1090 c          973 g          481 t
ORIGIN      
        1 ggccggactt gccggcgccg agctgcgcca gggcgatatg tacgcgctgc cgctcgcctc
       61 cggatcggcc gacacgatca tcatccacca ggtgctgcat tacgcccagc atcccgccgc
      121 cgccgtcgcc gaggcggcgc gcctgctcgc ccccggcggc cggctgctga tcgtcgattt
      181 cgcgccgcac gagcgcgagg agctgagggt gcgggacgcc catgtccggc tcggcttcgc
      241 cgacgatgcc gtagtgaagt ggatggccga aaccggcctc gacggccgcg tcgtcggcca
      301 tctcgagggc ggcgccctca ccgtcaccct gtggacgggc gaacagcccg aagcccgctt
      361 gaaagtcgtc gcatgagccc cgtcgcaccc gctttcgccg ccagcggcca gcctttgttc
      421 gagaatgtcg ccggcgacat cgacgtgtcg ttcgaattct tcccgcccaa gacggagaag
      481 atggaagaga cgctgtggga atcggtcaag acgctggagc cgctggcgcc gcgcttcgtc
      541 tcggtcacct acggcgccgg cggatcgacc cgcgcgcgca cccacgccac ggtcgagcgg
      601 atcgcgcgcg agaccagcct cgcggcggcc gcccacctga cctgcgtcga ggccagccgc
      661 gcggagattg acgaggtcgc gcgcgattat tgggcggccg gcatccgcca catcgtcgcc
      721 ttgcgcggcg acccgcccga acagggccgc agcttccagc cgcatcccga cggctatgcc
      781 aatgcggtcg agctgatccg cggcctcaag gcggtggcgc cgttcgagat ttcggtcgcc
      841 gcctatccgg aatgccaccc cgattcgccg aacaaggccg ccgacctgga caatctgtgg
      901 cgcaagatcg acgccggcgc ggaccgggcg atcacccaat tcttcttctc ggccgactgc
      961 ttcttccgct tccgcgacac ggcggcggcg atggggatcg aggccgagat cgtgcccggc
     1021 atcctgcccg tctcgaacgt cgcccagacg cgcaaattcg cggcgatgtg cggcgccacc
     1081 atcccgagct ggctcgaccg gctgttcgaa gggctggacg atctgccggc cgcgcgccag
     1141 ctggtcgccg ccaccgtcgc ggccgaattg tgcggccagc tttacgccgg cggcgtccgc
     1201 catttccatt tctacacctt gaaccgggcg gagcttgctt acgcgatctg ccacctgctc
     1261 gggctgcggc cgaaggaaaa atccaatgaa cgtcgcacag cagcttaagg ccgaagccgc
     1321 caagcggatc ctcgtcaagg acggcgccta tgggacgcag gtccaggcgc gcaggctggc
     1381 cgaggccgat tatcgcggcg ggctcgacct cggccgcgac cagaagggca ataacgacct
     1441 cctgaacctc acgcggcccg ccctggttgg cgccatcgcc gacagcttcg cctgcgccgg
     1501 ggccgacatc ctcgccacca acagcttcaa cgccaaccgg atcagccagg ccgattacgg
     1561 ggccgagacg ctggtgcgcg acatcaacgc cgccgccgcc cggatcatct gcgaggtcgc
     1621 cgaccggcac gcgcagaagg acgggcgcaa gcgctgggtg gcgggcgcgc tcgggccgac
     1681 caacaagacg ctgtcgctgt cgcccaacgt caacgatccg ggctttcgcg agatcgactt
     1741 cgacttcctg tccgacgtct atgttgagca gatcgaaagc ctggtcgagg gcggcgccga
     1801 gatcgtgctg atcgagaccg tgttcgacac attgaacgcc aaggcggcga tccacgccgc
     1861 cggccgcgtc gatcgcgagc tgccgatcat gctgtcgatg acgatcaccg atttgtccgg
     1921 ccgcaacctt tccggccatt cgatcgaggc cttctgggcg tcggtgcgcc acgcgcgccc
     1981 gctgtcgatc gggctcaatt gctcgttcgg ggcggcgcag ctgcgggcgc acgtcgccag
     2041 cctatcgggc atcgccgacg cgctgatcat ggcttatccc aatgccggcc tccccaacga
     2101 tctcggcgat tatgacgaat gcccggagac cacggcggcg caggtccgcg aatggtgcga
     2161 gcagggcatc gtcaacatcg tcggcggctg ctgcggcacg acacccgagc atatcgccgc
     2221 catcgcggaa atagtgtcca aatatccgcc gcggccgatc gccgcccccg tccgccgcac
     2281 catgctcgcc ggcatcgagc cgatgaactt ggcagcctaa acccctctcc ccttcagcgg
     2341 agaggccggg agaggggctg tcccctcgca cgcattagaa gattaaaaat gaccgccaca
     2401 gcctatctcc aggactcccc ctctccccaa ccctctcccc gcgagcgggg agagggagct
     2461 gtcagttcct ccttcgtcaa tatcggcgaa cggaccaacg tcaccggctc ggcccagttc
     2521 aagaagctga tcatggccgg cgactatgcc gccgcggtcg aggtggcgcg ccagcaggtg
     2581 gagaatggcg cccagatcat cgacatcaac atggacgagg gcctgctcga cgccgaatat
     2641 gccatgacca ccttcctgaa gctgatcgcg gccgagccgg acatcgcccg cgtcccgttc
     2701 atgatcgaca gctccaaatg gagcgtgatc gaggccggcc tcaaatgcgt gtcgggcaag
     2761 ccgatcgtca attcgatcag catgaaggaa ggcgaagccg agttcctcgc ccatgcccgc
     2821 aaggtccgcg actatggcgc cgcaatggtg gtgatggcgt tcgacgaggt tggccaggcc
     2881 gacacccagg cgcgcaaggt cgagatttgc gagcgcgcct ataagctgct ggtcggcgac
     2941 ggcttcccgc ccgaggacat catcttcgat cccaacatct tcgcggtggc gaccgggatc
     3001 gacgaacatc gcaattacgc cgtcgacttc atcgaggcct gccgggaaat ccgcgcccgc
     3061 tgtccgcacg cccatat
//
LOCUS       DASVRK010000085         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_200740, whole genome shotgun sequence.
ACCESSION   DASVRK010000085 DASVRK010000000
VERSION     DASVRK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200740"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VF589_02915"
     CDS             complement(<1..480)
                     /locus_tag="VF589_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256559.1"
                     /translation="MPICLRMLPFALLTFALPVFANSAVPVVEAERAFARDVSEQGFK
                     KGFLAHAAPDAIMFSPRPADPRPGLTALPDGPPPGAPLQWWPQWAGMATSGDFGFTTG
                     AASVPVRYFTVWRKQPDGSWKWIYDGGPPLKEKMKGGPDDKVTYLAPAGAAAGSERAA
                     "
     gene            689..>3070
                     /locus_tag="VF589_02920"
     CDS             689..>3070
                     /locus_tag="VF589_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8256560.1"
                     /translation="MIKGIAALSLALLSSTALAQDKDDKKKWNVAAPPMKTRAVPVNT
                     TEGTWMNLDVSPDGRTIAFDMLGDIYTVPIGGGRATRLAAGLPYETQPRFSPDGRQIA
                     FTSDRGGGDNIWLMNVDGSNKRQMTKETFNLLNNPSWSPDGRYIAARKHFTTYRSLGT
                     GEIWLYHVAGGKGVALVERPSKQHQKELGEPAFTPAGDAIYFSFDATPGPIFEYAQNS
                     NAEIYRIDRYNVKTGERSTVTGGPGGAVRPTPSPDGRYLAYVHRERAKSKLYVKDLRS
                     GEERKVYDDLDQDVQETWAVTGVYPNMDWTPDSRAIVFWAGGKIRRIGADGSGLAEIP
                     FQVNDTRDVIDPPRPNVQVWSETFTTRMPRFAQLSPDGRRVVFESLGRLYIKDAAGGA
                     PRPLTSADSDFQLFPAWSRDGRQIAFVSWNDQRLGEIRVVGPDGSGMRTVTRTPGHYR
                     RPRFSPDGRTLVYEAGEGGNLTSEAWSTNPGIFRVPAAGGEPVKLTSEGGNPHFGADN
                     DRIYMDLAEEQKVKLVSVDWNGKDRRVHAQGEMVIGYEVSPTGDQVAFRENYNVFLMP
                     FFAGAKTVDVTAKEGALPVTRASGVGGQYIHWTSGGRGLAWSLGPTLYQADAASLIQP
                     GAAYTAPRSGLSLAMPVRSDAPSGTVALVGAKIVTMADEAGGIVEDGIVIIEGNRIRA
                     VGRRGQVAIPAGATQVDVAGKTIIPGLIDAHAHGAQGEDDLIPQQNWEAMAHLAFGVT
                     TVHDPSSTSSEIFGSAEMQKAGVILAPRTLSTGEIVYGAKSPGRFAAIDSYEDA"
BASE COUNT          508 a         1165 c          946 g          451 t
ORIGIN      
        1 cgccgcccgc tccgatcccg ccgccgcgcc cgctggggcg agataggtga ccttgtcgtc
       61 cgggccgccc ttcatcttct ccttgagcgg cggcccgccg tcgtaaatcc atttccagct
      121 gccgtcgggc tgcttccgcc acaccgtgaa atagcgcacc gggaccgagg cggcgccggt
      181 ggtgaagccg aaatcgcccg acgtcgccat gcccgcccat tgcggccacc attgcagcgg
      241 cgcccccggc ggcggcccat ccggaagcgc cgtcagcccc gggcgcgggt ctgccggccg
      301 cggcgagaac atgatcgcgt cgggcgcggc atgggcgagg aagcccttct tgaacccctg
      361 ctcggagacg tcgcgggcaa acgcccgctc cgcctcgacc accggcacgg ccgaattggc
      421 gaacaccggc agggcgaatg tgagcagggc gaacggaagc attctgaggc agatgggcat
      481 ggcgtcgatc cttggctggc gacccgcgtc tagcttcggt ccgcgcgacc cgaacgcggc
      541 tttctgcgaa cggccgtgcc gctccgacga gcggctttgg tcgccgtgga atcaacggct
      601 tgcgcgccgc cgtgacttat tgcaggcgtt gcgccttccg ctttcaccag aatcacggcg
      661 ccgtcctggc gctttccgga gctcattcat gatcaaaggc atcgccgctc tgtcgctcgc
      721 cctcctgtcc tcgaccgcgc tcgcgcaaga caaggacgac aagaagaagt ggaacgtcgc
      781 cgcgccgccg atgaagacac gtgccgtccc ggtcaacacg accgaaggca cctggatgaa
      841 ccttgacgtc agccccgacg gccgcaccat cgctttcgac atgctcggcg acatctacac
      901 ggttccgatt ggcggcggcc gcgccacccg cctcgccgcg ggccttccgt acgagaccca
      961 gccgcgcttc tcgcccgacg gccgccagat cgccttcacc tccgaccgcg gcggcggcga
     1021 caatatctgg ctgatgaacg tggacggctc caacaagcgc cagatgacca aggagacctt
     1081 caacctcctc aacaatccga gctggagccc cgacggccgc tacatcgccg cccgcaagca
     1141 tttcaccacc taccgctcgc tcggtaccgg cgagatctgg ctctaccatg tcgccggcgg
     1201 caagggcgtc gctttggtcg agcgccccag caagcagcac cagaaggagc tcggcgagcc
     1261 cgccttcact ccggccggcg acgccatcta cttcagcttc gacgccaccc ccggcccgat
     1321 cttcgaatat gcccagaatt ccaacgccga gatttaccgc atcgaccgct acaatgtgaa
     1381 gaccggcgag cgttcgaccg tcaccggcgg ccccggcggc gccgtgcgcc cgaccccgtc
     1441 gcccgacggc cgctacctcg cctacgtcca ccgcgagcgc gccaagtcga agctctacgt
     1501 caaggatctg cgctcgggcg aggagcgcaa ggtctatgac gatctcgacc aggacgtgca
     1561 ggagacctgg gcggtgaccg gcgtctatcc gaacatggac tggacgcccg attcgcgcgc
     1621 catcgtcttc tgggccggcg gcaagatccg ccgcatcggc gccgacggca gcggccttgc
     1681 cgaaatcccg ttccaggtga acgacacccg cgacgtcatc gatccgccgc gccccaatgt
     1741 ccaggtgtgg agcgagacct tcaccacccg catgccgcgc ttcgcccagc tgtcgcccga
     1801 cggccgccgc gtcgtcttcg aaagcctcgg ccggctctac atcaaggacg ccgccggcgg
     1861 cgccccgcgc ccgctcacct ccgccgacag cgacttccag ctgttcccgg cctggtcgcg
     1921 cgacggccgc cagatcgcct tcgtctcgtg gaacgaccaa aggctcggcg aaattcgcgt
     1981 cgtcggccct gacggcagcg gtatgcgcac cgtcacccgc acgccgggcc attatcgccg
     2041 gccgcgtttc tcgcccgacg gtcgcaccct cgtctacgaa gcgggcgagg gcggcaacct
     2101 cacctccgaa gcctggtcga ccaaccccgg catcttccgc gttccggccg cgggcggcga
     2161 gccggtcaag ctcaccagcg agggcggcaa tccgcatttc ggcgccgaca acgaccgcat
     2221 ctacatggac ctcgccgaag agcagaaggt caagctggtc agcgtcgact ggaacggtaa
     2281 ggaccgccgc gtccacgccc agggcgagat ggtgatcggc tacgaagtct cgccgaccgg
     2341 cgaccaggtc gccttccgcg agaattacaa cgtcttcctg atgcccttct tcgccggcgc
     2401 caagacggtc gacgtcaccg ccaaggaagg cgcgctcccc gtcacccgcg ccagcggcgt
     2461 cggcggccaa tatatccact ggacgtccgg cggtcgcggc ctcgcctggt cgctcggccc
     2521 gaccctctac caagccgacg ccgccagcct gatccagccc ggcgccgcct acaccgcgcc
     2581 gcggtccggc ctgtcgctgg cgatgccggt ccgctccgac gcgcccagcg gcaccgtcgc
     2641 tttggtcggc gccaagatcg tcaccatggc ggacgaggcc ggcggcatcg tcgaggatgg
     2701 aatagtcatc atcgagggca accgcatccg cgcggtcggc cgccgcggcc aggtcgcgat
     2761 cccggccggt gcgacccagg tcgacgtcgc cggcaagacc atcatccccg gcctgatcga
     2821 cgcccacgct catggcgcgc agggcgagga cgacctcatc ccgcagcaga attgggaggc
     2881 gatggcgcat ctcgccttcg gcgtcaccac cgtccacgac ccgtcgagca cgtcgagcga
     2941 gattttcggc tcggccgaga tgcagaaagc gggcgtcatc ctcgcccccc gcacgctctc
     3001 gaccggcgag atcgtctatg gcgccaagtc gcccggccgc ttcgccgcga tcgacagcta
     3061 tgaggatgcg
//
LOCUS       DASVRK010000086         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_14061, whole genome shotgun sequence.
ACCESSION   DASVRK010000086 DASVRK010000000
VERSION     DASVRK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14061"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1873)
                     /locus_tag="VF589_02925"
     CDS             complement(<1..1873)
                     /locus_tag="VF589_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256561.1"
                     /translation="MATFTGTDASETINPSFVSPTVTSDGGSLPSAAADIINGGGGSD
                     TVEGGGGADQVTLGGGDDLFTWRFNDGDDILEGGAGSDRLDFYSVSNAAATGLELIQL
                     VNNGGRLRLSSSFAPDGALLDVNGFEEIVISAMEGPDTIIVSDLAPTGLTNLRINLSA
                     EVDGVAGDGDTDRLSLAGGSGDEFVQVSATAGGLAVSGLGAAIAIEAMEAGDFISLNG
                     AGGNDFLSADSAGRSVYLSGGAGNDKLVGSSAADTLRGDEGDDEVQGGTGDDDVQLGA
                     GDDSLLWFKGHGFDSVDGGADFDRIDLRGSGGTDTFAISAGPTDAAIEVDYAIALDIR
                     AVERVDFVDFGGSDLFSIGDLTGTSLRQIFLDVGFDTDLDLVVLHGGGGSDTVLLADI
                     AGRVRISGLAATVTVERLGRIDEIHVFAGNGADTIDASAAGEGPALLVLRGEDGDDRL
                     VGGARRESLSGGFGDDIVDYRGSDAGVTLLLDEGRGFGGDAAGDVLIEFEHAFGSAFA
                     DILEGSHVGNSLTGGAGGDQLYGHDGDDRLVGGAGADRSHGGIGDDLHFIDNVADVAR
                     ERQGEGIADRVLASVSYTLAGGSEIELLTTTNAAGLGGIDLTGNEYGNRVAGNAGANR
                     "
     gene            complement(2095..3123)
                     /locus_tag="VF589_02930"
     CDS             complement(2095..3123)
                     /locus_tag="VF589_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8256562.1"
                     /translation="MGRTLAWLGIAVLLAVAGPQTRSAASPAPQAQAAPLDRAERAAT
                     VAQLGEALHAMYLKPEVGQRYAFALQSNLAAGAYEDLTEPRAFGERLTKDLQSVAPDV
                     HLRLLPNEVFEHPPEEAGGGTSRFPEGIADMRMMGRVAYLRFTAFPHDPRTAPAARAF
                     LIAHAGDAKAVVIDARELPGGGLEVMDAMLPLFFSRPTLLMRLETRAAGDKESPFPDG
                     PTLVRREGRPGYVTRDHVVAPDPSETRLRRTPLYYLVSGKTASAGEHLALALKRTKRA
                     TLIGETTRGAGHYVALAPVGTSLTALIPVGRSFDPDTGWDWDGKGVAPDVAVPADDAL
                     QAALSRIG"
     gene            3377..3667
                     /locus_tag="VF589_02935"
     CDS             3377..3667
                     /locus_tag="VF589_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256563.1"
                     /translation="MTELSRVGGDAGRMSHPFSARNWSYRGVRGGDAVAAYNLAMEGF
                     NRGDLQDYRRWLRRAARMGDEDAALQLKRFETRLPHETAYESGRGRPWRRRD"
BASE COUNT          573 a         1342 c         1314 g          591 t
ORIGIN      
        1 gccggttggc gccggcattg cccgccacgc ggttgccata ttcgttaccg gtgagatcga
       61 tgccgcccag ccccgccgcg ttggtggtgg tcagcaattc gatctccgac cctcccgcca
      121 gcgtgtagga gacgctggcg agcacccggt cggcaatgcc ttcgccctgc cgttcccggg
      181 cgacgtcggc gacattgtcg atgaagtgaa ggtcgtcgcc aatgccgcca tggctgcggt
      241 cggcgccggc gccgccgacc agccggtcgt cgccgtcatg gccgtaaagc tggtcgccgc
      301 cggcgccgcc ggtgaggctg ttgccgacat ggcttccctc gaggatgtcg gcgaaggccg
      361 agccgaaggc gtgctcgaat tcgatcagca catcgccggc ggcatcgccg ccgaagccgc
      421 ggccctcgtc gagcaacagg gtgacgccgg cgtcggagcc gcgataatcg acgatatcgt
      481 cgccgaaccc gcccgacagg ctctcgcgcc tcgcaccgcc caccagcctg tcgtccccgt
      541 cctcgcccct cagcaccagc aaagccggcc cttcgccggc cgcggacgcg tcgatcgtgt
      601 cggcgccgtt gccggcgaag acatggattt cgtcgatcct gcccaggcgc tcgacggtga
      661 ccgtcgccgc gaggccgctg atcctgaccc tgccggcaat gtcggcgagc aggaccgtgt
      721 ccgatccccc gccgccgtgg agcacgacca ggtcgaggtc ggtgtcgaac ccgacgtcga
      781 ggaagatctg gcgcagcgag gtgccggtca ggtcgccgat cgagaaaagg tcgctgccgc
      841 cgaagtcgac gaaatcgacc cgctcgaccg cacgaatgtc gagcgcgatc gcatagtcga
      901 cctcgatcgc cgcatcggtc ggcccggcgc tgatggcgaa cgtgtcggtg ccgccgctgc
      961 cgcgcaggtc gatgcggtcg aaatcggcgc cgccgtcgac cgagtcgaac ccgtggccct
     1021 tgaaccaaag caagctgtcg tcgccggcgc cgagctggac gtcgtcgtcg ccagtgccgc
     1081 cttggacttc gtcgtcgcct tcgtcgcccc tgagggtgtc ggcggccgag cttcccacca
     1141 atttatcgtt gccggcgccc ccgctcaggt aaaccgagcg gccggcgctg tcggcggaca
     1201 ggaagtcgtt gccgccggcg ccgttgaggc tgatgaaatc gcccgcctcc atcgcttcga
     1261 tcgcgatcgc cgcgccgagg ccgctgacgg cgaggccgcc ggcggtggcg cttacctgca
     1321 cgaactcgtc gccgctgccg ccggcgaggc tgagcctgtc ggtgtcgccg tcgccggcta
     1381 ccccgtccac ctcggcggaa aggttgatgc gcagattggt gaggccggtg ggcgcgaggt
     1441 ccgagacgat gatcgtgtcc gggccctcca tcgccgagat cacgatctcc tcgaagccgt
     1501 tgacgtcgag caaagcgccg tcgggcgcga acgaggagga aagccgcagc cggcccccat
     1561 tgttgaccag ctgtatgagc tcaagcccgg tcgcggccgc gttgctgacc gaatagaaat
     1621 cgagccggtc ggagccggcg ccgccttcca gtatgtcgtc gccgtcgttg aaccgccagg
     1681 tgaacagatc gtcgcccccg ccgagggtga cctggtcggc gccgccgccg ccttcgaccg
     1741 tgtcactgcc gccgccgcca ttgatgatgt ctgccgccgc cgacggcagc gatccgccgt
     1801 cggacgtgac cgtcggggaa acgaagctgg ggttgatggt ctcgctggcg tcggtgccgg
     1861 taaatgtcgc catgttcgtc tccgttcgga gatcggaggt gcgcacatct cacgccgggg
     1921 tggcatcatc ggcgctcgcg gcccctgtcg accttcatca tgcggtcagc gtcgcagcgg
     1981 gagggcaggg ggtgccactt ttccccctca gccgcgcacc ttagctcagc atcgacccga
     2041 tgacgagtcc cggagccgat ccattcgcgc ccgcggccgg cgcggggcgg gccttcaccc
     2101 gatccggctc aacgccgctt gcaatgcgtc gtcggcaggc acggcaacgt ccggcgcgac
     2161 gccttttccg tcccagtccc agccggtatc ggggtcgaag gaacgcccga ccgggatgag
     2221 cgcggtgagg ctggtgccga ccggggcaag cgcgacgtaa tggcccgcgc cgcgcgtggt
     2281 ttcgccgatc aaggtcgcgc gcttggtgcg cttcagcgcc agcgccaaat gctcaccggc
     2341 cgatgccgtc ttccccgaaa ccagataata aagcggcgta cgccgcaatc gcgtctcgga
     2401 cgggtcgggc gccacgacat ggtcgcgcgt cacatagcca gggcgcccct cccggcgaac
     2461 caacgtcggc ccgtccggga acgggctttc cttgtcgccc gccgcccgcg tctcgagccg
     2521 catcagcaag gtcgggcgcg agaagaataa ggggagcatc gcgtccatca cctccaggcc
     2581 tccgcccggc agctcgcgcg catcgatcac gaccgccttc gcgtctcccg catgggcgat
     2641 gaggaaggcg cgcgccgcgg gggcggttcg cgggtcgtgc ggaaaggcgg tgaagcgcag
     2701 ataggcgacc cgccccatca tccgcatgtc ggctatgccc tcggggaagc gggacgtgcc
     2761 gccgccggct tcctcgggcg ggtgctcgaa cacttcgttc ggcagcagtc gcaggtggac
     2821 gtccggcgcg accgactgaa gatccttggt cagccgctcg ccgaacgccc tcggctcggt
     2881 caaatcctca taagcgccgg ctgccaggtt cgactgcagc gcgaaggcat atctctgccc
     2941 gacctccggc ttcaagtaca tggcgtgaag cgcctcgccc agttgcgcaa ccgtcgcggc
     3001 gcgctcggcc cggtcgagcg gcgcggcctg cgcctgcggg gcgggcgaag cagcggagcg
     3061 cgtttgcggg cccgccacag caagcagcac cgcgatgcca agccacgcca atgttcgtcc
     3121 catccggtgg atcctcgaaa tcgttcgcca agcggtgcac gaacttgggt ccgctttcca
     3181 cccgtcccgg accttccacg gctttggctg gcggcgcggg attgagccgc tcgcccgtca
     3241 tcgctaatcg gaagcggtga gccactcccg caaatcccgt ctcgaggcca agacccaggc
     3301 ctggatcgaa gactgctcgc cgagcagacg ctcgcgcctg cttcggcacc tcgccttgcg
     3361 cggggagcca atggcgatga ccgagctcag ccgcgtcggc ggggacgcag ggcggatgtc
     3421 gcatcctttc tccgcccgca actggagcta tcggggggtt cggggcggcg atgcggtcgc
     3481 cgcctacaat ctggcgatgg aaggcttcaa ccggggcgac ttgcaggact accggcgctg
     3541 gctgcggcgg gcggcccgga tgggcgacga agacgcggcg ctgcaattga agcggttcga
     3601 gacccgcctc ccccacgaga cggcttatga atccggccgc ggccggccct ggcgcaggcg
     3661 agattgagcg acggcacgcc gggcggaagg ccgatcacgg cggccgttcc gctcgttgcg
     3721 gaccgcgccg gcctcgggca tgctgctccg atggcgatag ggctgtgcgg cggctgcggc
     3781 tgcgagatcg agggcggcgg gagcggctgc aggagccggt
//
LOCUS       DASVRK010000087         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_512385, whole genome shotgun sequence.
ACCESSION   DASVRK010000087 DASVRK010000000
VERSION     DASVRK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512385"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VF589_02940"
     CDS             <1..839
                     /locus_tag="VF589_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8256564.1"
                     /translation="REAKAVFHMAAQVAVTTSIVDPREDFEINLRGTINLLDALRLKG
                     DGTPVIFASTNKVYGDLADIDFRLEGQRYVPQDDAVRERGISEKRPLDFHTPYGCSKG
                     AADQYVLDYSRSFGIPSAVVRMSCIYGRRQMGTEDQGWVAHFLIRALRGEPITLYGDG
                     YQVRDILDVSNAVEAYLKAWANIDRISGRAFNLGGGPANAVSLRELIAHMSDLLGREI
                     DVRFEDWRAGDQRYFVADTGLIRSELGLAPEVPWKQGVAALAEWLRESRGLSAASEAV
                     PA"
     gene            836..2095
                     /locus_tag="VF589_02945"
     CDS             836..2095
                     /locus_tag="VF589_02945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8256565.1"
                     /translation="MKEELPRSPAKAGAQGGRDWTPAFAGEQKGAGEQEGAGEQKGAG
                     EQKAGAAAPLRLLLTTDAVGGVWQYSVDLARGLAGHGVETVLLVIGPSPSKAQLKQAA
                     GIDGATLVDTGLPLDWVARTRGSVRNAGAAIAKLAASHAVNLVQLHAPALAAEAKFPV
                     PVVAFAHSCLATWWQAVKQTEVDGAWKWRSDLVAKGYRAADLVVAPSAAFAAATHAAH
                     GLEAPPLTVHNGRSPLALPRVASHDFAFTAGRLWDKAKNVRTLDAAAGRLAVPFYAAG
                     PERGPSGDAIALEHARGLGSLGEKEVARWLAARPVFASAAVYEPFGLAVLEAAAAGCP
                     LVLSDIPTFRELWEGAATFVDAMDDKGFAAAIGRIIGDDLLRAQLGAAAKERAARYTP
                     DAMAARMAELYRRLAAKPADPAEQVAA"
     gene            2092..>2707
                     /locus_tag="VF589_02950"
     CDS             2092..>2707
                     /locus_tag="VF589_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8256566.1"
                     /translation="MRIVYFTHSLASCWNHGNAHFLRGVLRELTLRGHDVRAYEPEHS
                     WSLKNLLDDHGGKGLDAYKAAYPELSSRLYGPAGKLERMLDGADLVIVHEWNEPSLVA
                     EIGRLRARGGRFSLLFHDTHHRAVSDPEAIRAFDLFGYDGVLAFGETLSEVYRRWGWA
                     GRVWTWHEAADIRLFHPPAEEGPREGLVWVGTWGDGERTAELEEF"
BASE COUNT          386 a          943 c          958 g          420 t
ORIGIN      
        1 cgcgcgaggc caaggccgtg ttccacatgg ccgcccaggt ggcagtcacc acctccatcg
       61 tcgatccgcg cgaggatttc gagatcaatc ttcgcgggac gatcaacctg ctcgacgcgc
      121 tgcggctgaa gggcgacggt acgccggtga tcttcgcctc caccaacaag gtctatggcg
      181 acctcgccga tatcgacttc cggctcgagg gccagcgcta cgtgccgcag gacgatgcgg
      241 tgcgggagcg cggcatttcg gaaaaacgcc cgctcgattt ccacacgcct tatggctgct
      301 ccaaaggcgc ggccgaccaa tatgtgctcg attattcgcg cagcttcggc attccctcgg
      361 cggtcgtccg gatgagctgc atctacggcc ggcggcagat ggggaccgag gaccagggct
      421 gggtcgcgca tttcctgatc cgcgcgctcc gcggcgagcc gatcaccctt tacggcgacg
      481 gctatcaggt gcgcgacata ctcgacgtgt ccaacgcggt ggaggcctat ttgaaggcgt
      541 gggcgaatat cgaccggatc tcggggcgcg ctttcaattt gggcggcggg ccggccaatg
      601 cggtgtcgct gcgcgagctc atcgcccaca tgtccgacct gctcgggcgc gagatcgacg
      661 ttcgcttcga ggattggcgc gcgggcgacc agcgttattt cgtcgccgat accggcctca
      721 tccgctccga actgggcctc gcccccgagg tgccgtggaa gcagggcgtc gcggctctgg
      781 ccgagtggct ccgcgagtcg cgcggcctca gcgccgcgag cgaggcggtg ccggcgtgaa
      841 ggaagagctt cctcgttccc cggcgaaggc cggggcccag ggtgggcgag actggacccc
      901 ggccttcgcc ggggagcaga agggcgccgg ggaacaggag ggcgccgggg agcagaaggg
      961 cgccggggag cagaaggcag gcgccgcagc cccgctccgc cttctcctca ccaccgacgc
     1021 ggtcgggggg gtgtggcaat attcggtcga tctggcccgc ggcctcgccg gccatggcgt
     1081 ggaaaccgtc ctgctcgtca tcggcccctc gccgagcaag gcgcagctga agcaggcggc
     1141 ggggatcgac ggcgcgactt tggtcgatac cgggctgccg ctcgactggg tcgcccgcac
     1201 ccgcggctcg gtccgcaatg ccggcgccgc catcgccaag ctggcggcga gccacgcggt
     1261 caacctcgtc cagctccacg cgcccgcgct ggcggccgag gcgaagtttc cggtcccggt
     1321 cgtcgccttc gcgcatagct gcctcgccac ctggtggcag gcggtcaagc agacggaggt
     1381 ggacggcgcc tggaaatggc gctccgacct ggtcgccaaa ggctatcgcg ccgccgacct
     1441 ggtggtcgcg cccagcgccg ccttcgccgc ggcgacccat gccgcccatg gcctcgaagc
     1501 gccgccgctc accgtccata acgggcgctc gccgcttgcc ttgccgcggg tggcgtcgca
     1561 cgatttcgcg ttcacggcgg ggcggctgtg ggacaaggcc aagaatgtcc gcaccctcga
     1621 cgccgccgcc gggcggctgg cggtgccctt ctacgcggcc ggccccgagc gcggcccgag
     1681 cggcgatgcg atcgcgctcg aacatgcgcg cgggctcggg tcgctgggcg agaaggaggt
     1741 cgcgcgctgg ctggcggcgc ggccggtgtt cgcctcggcc gcggtctacg agccgttcgg
     1801 gctcgccgtg ctcgaagccg ccgcggccgg atgcccgctc gtcttgtcgg acatccccac
     1861 cttccgcgaa ttgtgggaag gcgccgccac cttcgtcgat gcgatggacg ataaaggctt
     1921 cgccgccgcg atcggccgca tcatcggcga cgatttgctg cgcgcccagc tcggcgccgc
     1981 ggccaaggag cgggccgcac gctatacgcc cgatgccatg gctgcgcgga tggcggagct
     2041 ctaccggcgg ctcgcggcca agccggccga cccggccgag caggtggcgg catgaggatc
     2101 gtctatttca cccattcgct ggcgagctgc tggaaccacg gcaacgcgca tttcctgcgc
     2161 ggcgtgctgc gcgagctgac gctgcgcggc cacgacgtcc gggcttatga gcccgagcat
     2221 agctggagcc tcaagaacct gctcgacgat catggcggca aggggctcga tgcctataag
     2281 gccgcttatc ccgagctttc ctcgcggctc tatggcccgg ccggcaagct cgaacggatg
     2341 ctggacggcg ccgacctcgt catcgtccac gaatggaacg agccgtcgct ggtcgccgag
     2401 atcggccggc tgcgggcccg cggcgggcgg ttcagcctgc tgttccacga cacccatcac
     2461 cgcgcggtca gcgatccgga ggcgatccgc gccttcgacc tgttcggcta tgacggcgtg
     2521 ctcgccttcg gggaaaccct gtccgaagtc tatcgccgct ggggctgggc cggccgcgtc
     2581 tggacctggc acgaagccgc cgacatccgc ctgttccacc cccccgccga ggaaggtccg
     2641 cgcgagggtc tggtctgggt cggcacttgg ggcgatggcg agcgcaccgc ggagctcgag
     2701 gagttcc
//
LOCUS       DASVRK010000088         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_14508, whole genome shotgun sequence.
ACCESSION   DASVRK010000088 DASVRK010000000
VERSION     DASVRK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14508"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VF589_02955"
     CDS             <1..717
                     /locus_tag="VF589_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX8256567.1"
                     /translation="VVNLVGILKGNFQAIHIEGARTIAQAAAAAGAGSLVHTSAIGAD
                     PDHPSAYGRSKALGERAVRGAFPSATIMRPSVVFGREDNFINRFAGMARLLAVLPVIR
                     GEAKLQPVFAADVGHAVAAAALDPAAHAGRTYELGGPQIMTMRELNVWIGEATARNPA
                     LIDVPDSVGRMLVQLFGWLPGAPITGDQWLMLQRDNIAAEGAEGLRSFGITPTPLAAV
                     ADSWLTSYRRNGRFGAKQPY"
     gene            729..1538
                     /locus_tag="VF589_02960"
     CDS             729..1538
                     /locus_tag="VF589_02960"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX8256568.1"
                     /translation="MPLILTAILLGIVEGLTEFLPVSSTGHLILAAELLGYRAEQWAT
                     FNVIIQLGAILAVIVLYWRTFWTVATGLFARDAGAWRFALNVAVAFLPSAVLGLLLID
                     NIEALLGNAIVVAVALILGGIAILLIERWVKPASLEPGVAAIPLKRALGVGLVQCLSM
                     VPGVSRSGATIMGALALGVERRTAAEFSFFLAVPTMVGATGLSVFKHHEEIAAAGIDW
                     GLIAIGFVVSFVVAIAVIKWFVGIVSRHGFAPFAWYRIVAGAAALAWLLAR"
     gene            1605..>1868
                     /locus_tag="VF589_02965"
     CDS             1605..>1868
                     /locus_tag="VF589_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256569.1"
                     /translation="MSAAVLLALGALAAAAPPAAPPGTARPAPPPIVKVVPAPPGAAP
                     VDPARLAAPQRMMLQILPPGTYAKLTGGSVRTIVEAAAAGAFHD"
BASE COUNT          231 a          702 c          625 g          310 t
ORIGIN      
        1 gtcgtcaacc tggtcggcat cctcaagggc aacttccagg cgatccacat cgaaggggcg
       61 cgcaccatcg cccaagccgc ggcggcggcc ggcgccgggt cgctggtcca cacgtcggcg
      121 atcggcgccg atcccgacca cccttccgcc tacggccgca gcaaggcgct cggcgagcgg
      181 gcggtccgcg gcgccttccc ttcggcgacg atcatgcgcc cgtcggtggt gttcgggcgc
      241 gaggacaatt tcatcaaccg cttcgccggc atggcgcggc tgctggcggt gctgccggtg
      301 atccggggcg aggcgaaact gcagccggtc ttcgccgccg acgtcggcca cgcggtcgcc
      361 gccgccgcgc tcgacccggc cgcccatgcc ggccgcacct atgagctggg cgggccacag
      421 atcatgacga tgcgcgaact gaacgtgtgg atcggcgagg cgaccgcccg caaccccgcc
      481 ctcatcgacg tccccgacag cgtcggccgg atgctcgtcc agctgttcgg ctggctgccc
      541 ggcgcgccga tcaccggcga ccagtggctg atgctgcagc gcgacaatat cgccgccgaa
      601 ggcgccgagg ggctgcgcag cttcggcatc acgccgacgc cgcttgcggc cgtggccgac
      661 agctggctca cctcctaccg ccgcaacggc cgcttcggcg ccaagcagcc ttactaagga
      721 cgccccactt gcccctgatt ctgacggcga tcctgctcgg catcgtcgag ggactgaccg
      781 agttcctgcc ggtctcctcg accgggcatc tgatcctcgc cgccgaattg ctgggctacc
      841 gcgccgagca atgggcgacg ttcaacgtca tcatccagct cggcgcgatc ctggccgtga
      901 tcgtgcttta ctggcgcacc ttctggaccg tcgccaccgg cctgttcgcg cgcgacgccg
      961 gcgcctggcg gttcgcgctc aacgtggcgg tcgccttcct gccgtcggcg gtgctgggcc
     1021 tcctattgat cgacaatatc gaagccttgc tcggcaacgc catcgtcgtc gccgtcgccc
     1081 tgatcctcgg cggcatcgcc atcctgctga tcgagcgctg ggtgaagccc gccagcctcg
     1141 agccgggcgt ggcggcgatc ccgctgaagc gggcgctcgg cgtcgggctg gtccagtgcc
     1201 tgtcgatggt gccgggggtc agccgttcgg gcgcgaccat catgggcgcg ctcgcgctcg
     1261 gcgtcgagcg ccggaccgcg gccgagttca gcttcttcct cgccgtcccg accatggtcg
     1321 gcgcgaccgg cctgtcggtg ttcaagcatc atgaggagat cgccgccgcc gggatcgact
     1381 ggggcctgat cgcgatcggc ttcgtggttt cgttcgtcgt cgcgatcgcg gtcatcaaat
     1441 ggttcgtcgg catcgtctcg cgccacggct tcgccccctt cgcctggtac cggatcgtcg
     1501 ccggcgccgc cgcgctcgcc tggctgctgg cgcgctgaac ctgccggctt gatctcctcc
     1561 gggagaggtg cgaaccttct aagtcgctca agcgggagga ttagatgtca gccgccgtgc
     1621 ttctggctct cggggcgctc gccgcggccg cgccgcctgc cgcgccgccc ggcacggctc
     1681 gcccggctcc tcctccgatc gtcaaggtcg tgcccgcacc gcccggggcg gcgccggtcg
     1741 atccggcccg gctcgccgcc ccccagcgca tgatgctgca gatcctgccg cccggaacct
     1801 atgccaagct gaccggcgga tcggtgcgga cgatcgtcga ggccgccgcc gcgggcgcct
     1861 tccacgac
//
LOCUS       DASVRK010000089         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_77485, whole genome shotgun sequence.
ACCESSION   DASVRK010000089 DASVRK010000000
VERSION     DASVRK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77485"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VF589_02970"
     CDS             complement(<1..606)
                     /locus_tag="VF589_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8256570.1"
                     /translation="MVKHLILAGVAALSVAVATSPGWAAEAGAAGSAAAASKPQLGSW
                     GVDTQGMDKNVRPGESFFEYVNGNWARTTEIPADKSSWGGFGMLRDLSDQRTRAIIEE
                     AAASRGAPGSNARKVGDFYASFMDEAAIEAKGAEPLKPYFAKIEAIRSSADLGRAFGE
                     AARMGIDVPIGPQVEQDLKDNSRYTVYVGQGGLGLPDRDYYL"
     gene            complement(783..1286)
                     /locus_tag="VF589_02975"
     CDS             complement(783..1286)
                     /locus_tag="VF589_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX8256571.1"
                     /translation="MIAFKLGRCGHAFAVLAAAGLVSSAPVLADTPTGSAAIAGATAQ
                     PFKPIVTTPRMRAEAATKLQAVEALYEQVGEGEASYYGHELAGNRTASGERFNPYALT
                     AAHRTLPLGSKLRVTNKSNGKSVVVRINDRGPFTHRRVLDLSLGAAKQIDMFRAGKAR
                     VRLELLR"
BASE COUNT          255 a          466 c          548 g          272 t
ORIGIN      
        1 gagataataa tcgcggtcgg gaaggccgag gccgccctgc ccgacataga ccgtgtagcg
       61 gctattgtcc ttgaggtcct gctcgacctg cggcccgatc ggcacgtcga tgcccatccg
      121 cgccgcttcg ccgaacgcgc ggccgaggtc cgcggaggag cggatcgcct cgatcttggc
      181 gaaatagggc ttgagcggct cggcgccctt cgcctcgatc gcggcttcgt ccatgaagct
      241 ggcgtagaaa tcgccgacct tgcgggcgtt gctgccgggc gcgcccctgg acgcggccgc
      301 ttcctcgatg atcgcgcggg tgcgctggtc ggaaaggtcg cggagcatcc cgaagccgcc
      361 ccagctcgac ttgtcggcgg ggatttcggt ggtccgcgcc caattgccgt tgacgtactc
      421 gaagaagctc tcgcccgggc ggacattctt gtccatgccc tgggtgtcga cgccccagct
      481 gccgagctgc ggcttcgaag cggcggcggc gctgccggcc gcgccggctt cggcggccca
      541 gcctggcgac gtggcgacgg cgacgctgag cgcggcgacg ccggccaaaa tcaaatgttt
      601 gaccatatta ccctcttccc ggtgtgtgat gatgctcaca cactaaggct tgggaacgat
      661 cttgggaagg gggagcgggt gtgcgcccga atggaatcgt tccccggcga aggccggggt
      721 ccagacccaa ccatcctctg gaccccggct ttcgccgggg agcagccgcg gccgcggaga
      781 acttagcgga gcagctcgag ccggacgcgg gccttgccgg cgcggaacat gtcgatctgc
      841 ttggcggcgc ccagcgagag gtcgagcacg cggcggtggg tgaaggggcc gcgatcgttg
      901 atgcggacga cgacgctctt gccgttgctc ttgttggtga cgcgaagctt gctgccgagc
      961 ggcagggtgc ggtgggcggc ggtgagcgca taaggattga agcgctcgcc gctggcggtg
     1021 cggttgccgg ccagctcatg gccgtaatag ctggcctcgc cctcgccgac ctgttcgtac
     1081 agcgcttcga cggcttgcag cttggtggcg gcttcggccc gcattcgcgg cgtggtcacg
     1141 atcggcttga acggctgcgc ggtcgcgccg gcgattgcgg cggagccggt cggcgtgtcg
     1201 gcgagcaccg gcgccgagga gaccaaaccg gcggcggcca gcacggcaaa ggcatggccg
     1261 cagcggccaa gcttgaaagc gatcatggaa gtgccccttt agctcgttgg gaagaggcat
     1321 aattgaggcg gcttaacgag tcccacccga gtcgatgttc cgagccgtca gcgggacgaa
     1381 cgacataatc aaggttaata agagggaact tactcttatc gacatgaaaa ggtgttgctg
     1441 tcactttggc ggcgatgcgt gagaaggaac tgaggctggc ggcgatttgc tacggtggca
     1501 tcagccttgc cgtctacatg catggaatta ccaaggaaat c
//
LOCUS       DASVRK010000090         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_450617, whole genome shotgun sequence.
ACCESSION   DASVRK010000090 DASVRK010000000
VERSION     DASVRK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450617"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /gene="smpB"
                     /locus_tag="VF589_02980"
     CDS             <1..347
                     /gene="smpB"
                     /locus_tag="VF589_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX8256572.1"
                     /translation="FGEGSIAESYAEVKDGEVWLINSNVPEFSHGNRFNHEPKRPRKL
                     LLHQREISKLHGAVARQGMTLVPLSIYFNGRGRAKVELALARGRKAPDKREYQKEKDW
                     KREQGRLMRERG"
     gene            340..900
                     /locus_tag="VF589_02985"
     CDS             340..900
                     /locus_tag="VF589_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX8256573.1"
                     /translation="MVERLSRWFAKNTPKREDLAESRWIKPFGGRVLRSELWRFTRRS
                     VPRGVGVGLLVGIFLMIPGLQIIGAALLSVPLRANIPIAAAMTFLSNPATTPFILLAS
                     IFVGNKLGFHADTGAFGALYARRAGPAEWFAWLFSDAAPALVCGLFIIAFASAAIGYF
                     VSGFVWRWWQGRKWRRRGERAREVLP"
     gene            978..>2534
                     /locus_tag="VF589_02990"
     CDS             978..>2534
                     /locus_tag="VF589_02990"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8256574.1"
                     /translation="MASFAQSASAPARRFDPFAAAIVALAAASAGLVLWTVGDLLFAG
                     AFFAALAGVAAILLLRPRAADSARSDEGTASIDLALLRAALDSAAAALAVTDESGRMI
                     CANELYSDWFGGAAAPADLPLRGDDSDRLAEAGAAARRDGKAEIDRLQVGGLTVSAEV
                     AQAGFGSAYLLYRFTRSEDLDFVREAQRLLTGEPGQRIGQAGVMAALTDGQGGLITAN
                     RAFAARALGRADAPLAGGQLVELLAATEHGQFLFAAEGKSATPLRIVQIPLAGGHDPL
                     TLFLMLDETAGFGRAAAEGEAASVNALLDFLPLGLALANVDGRFIYLNKAFRKAAGLP
                     RDARPVYPGDLVVEEDKGAISDAVRRFARGPTTSSDMAVRLRTNPDEPVALTIAGARG
                     LGEAAVILSLKDNSEEDRLKRQVAQATKMQAVGQLAGGVAHDFNNILTAILGYCDLML
                     MRHTPGDSDYDDIQQIRSNSNRAAGLTRQLLAFSRQQTLRPQVLQLPDVISEVSNLLK
                     RLLGETVKLQV"
BASE COUNT          351 a          953 c          828 g          402 t
ORIGIN      
        1 ggttcggaga aggctcgatc gctgaaagct atgccgaggt gaaggatggc gaagtgtggc
       61 tcatcaattc caacgtcccg gaattcagcc acggcaatcg cttcaaccat gagcccaagc
      121 ggccgcgcaa gctgctgctc caccagcgcg agatttccaa gctgcacggc gcggtggcgc
      181 gccagggcat gacgctcgtc ccgctctcca tctatttcaa cggccgcggc cgcgccaagg
      241 tcgagctcgc gctcgcccgc ggccgcaagg cgcccgacaa gcgcgaatat cagaaggaaa
      301 aggactggaa gagggagcag ggccggctga tgcgcgaacg tggttgaacg gctctcccgc
      361 tggttcgcga agaacacgcc caagcgcgag gatctggccg aaagccgctg gatcaagccg
      421 ttcggcggcc gcgtgctgcg cagcgaattg tggcgcttca cccggcgttc ggtgccgcgc
      481 ggcgtcggcg tcggcctgct ggtcggcatc ttcctgatga tcccgggcct tcagatcatc
      541 ggcgcggccc tgctgtcggt gccgctgcgc gccaacatcc cgatcgccgc ggcgatgacc
      601 ttcctgtcca acccggcgac cacaccgttc atcctgctcg cctcgatctt cgtcggcaac
      661 aagctcggct tccacgccga caccggcgcc ttcggtgcgc tctacgcccg ccgcgccggg
      721 ccggccgaat ggttcgcctg gctgttctcg gacgccgcgc cggctttggt ctgcggcctg
      781 ttcatcatcg ccttcgcttc ggctgcgatc ggctatttcg tctccggctt cgtctggcgc
      841 tggtggcagg gccgcaagtg gcggcggcgg ggggagcggg cccgggaagt gcttccctaa
      901 ccgcgcggcg caccgccgct ctcgggcagg cgcaggccgc ttgacggcgg cgggggcggc
      961 aggggatgac gaagcccatg gcaagtttcg ctcagtccgc ttccgccccc gctcggcgct
     1021 tcgatccgtt cgccgccgcg atagtggcgc tcgccgccgc ctcggccggg ctggtgctct
     1081 ggacggtcgg cgacctgctg ttcgccggcg cctttttcgc cgcgctcgcc ggcgtcgccg
     1141 ccatcctcct gctccgcccc cgcgccgcgg attccgcccg gtccgatgag ggcaccgctt
     1201 cgatcgatct cgcattgctg agggccgcgc tcgacagcgc tgccgccgcg cttgcagtca
     1261 ccgacgaatc gggccggatg atctgcgcca acgagctcta ttcggactgg ttcggcggcg
     1321 ccgccgctcc cgccgacctg ccgctgcgcg gcgacgattc cgatcggctc gccgaggccg
     1381 gcgccgccgc ccgccgcgat ggcaaagccg agatcgaccg gctccaggtc ggcggcctca
     1441 ccgtctcggc ggaggtcgcg caggccggct tcggcagcgc ctatctcctg taccgcttca
     1501 cccgctcgga ggatctcgac ttcgtgcgcg aggcgcagcg gctgctcacc ggcgagcccg
     1561 gccagcgcat cggccaggcc ggcgtgatgg cggcgctgac cgacggccag ggcggcctca
     1621 tcaccgccaa ccgcgccttc gccgcgcgcg cgctcggccg cgccgacgcc cccctcgccg
     1681 gcggccagct cgtcgaactg ctcgccgcca ccgagcacgg ccagttcctg ttcgctgccg
     1741 agggcaaaag cgcgaccccc ttgcgcatcg tccagatccc gctcgccggc ggccacgacc
     1801 cgctcacttt gttcctgatg ctcgacgaga ccgccggctt cggccgcgcc gccgccgagg
     1861 gcgaggccgc cagcgtcaac gccttgctcg atttcctgcc gctcggcctc gcgctcgcca
     1921 atgtcgacgg ccgcttcatc tatctgaaca aggcgttccg gaaggcggcc ggcctgccgc
     1981 gcgacgcccg cccggtctat cccggcgacc tcgtggtcga ggaggacaag ggcgccatct
     2041 ccgatgcggt gcgccgcttc gcccgcggcc cgaccacctc cagcgacatg gcggtgcgcc
     2101 tgcgcaccaa tcccgacgag ccggtcgcgc tcactatcgc cggcgcccgc ggcctcggcg
     2161 aggcggcggt catcctctcg ctcaaggaca atagcgagga ggaccggctg aagcgccagg
     2221 tcgcgcaggc gaccaagatg caggcggtcg gccagctcgc cggcggcgtc gcccacgatt
     2281 tcaacaatat cctgaccgcc atcctcggct attgcgacct catgctgatg cgccacaccc
     2341 cgggtgacag cgattatgac gacatccagc agatccggag caattcgaac cgggccgccg
     2401 gcctgacgcg ccagttgctc gccttctcgc gccagcagac gctgcgcccg caagtcctgc
     2461 agctcccgga cgtcatttcc gaagtctcga acctcctgaa gcgcctgctc ggcgagacgg
     2521 tgaagcttca ggtg
//
LOCUS       DASVRK010000091         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_388209, whole genome shotgun sequence.
ACCESSION   DASVRK010000091 DASVRK010000000
VERSION     DASVRK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388209"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VF589_02995"
     CDS             complement(<1..639)
                     /locus_tag="VF589_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ImuA"
                     /protein_id="HEX8256575.1"
                     /translation="MPLSPARAERLAALRAEVRAIESSTAKAGRECLPFGVDAIDRRL
                     EGGGLALAALHEITGARPDLSDDAAATLFVAGIAARRPGTVLWALSRRDLFAPGLALA
                     GLPPDRILYAECGRDEDVLAVMEEGLRHGGLAAVVGEAARVPMAAARRLQLAAEEGGA
                     MALMLKRWRRNGEDPLAAPSSAVTRWRIACAPSSPLPVPGVGRARWRLVLARQ"
     gene            825..1133
                     /locus_tag="VF589_03000"
     CDS             825..1133
                     /locus_tag="VF589_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX8256576.1"
                     /translation="MPNPLLKGLRPVHPGELLREDVLPALGRTKAEIARLLGISRQTL
                     YDILNERQPVTPAMAHRLAKLLGGSREAWLNMQRNCDLAAAERELADALAAIPTLKAA
                     "
     gene            1222..>2530
                     /locus_tag="VF589_03005"
     CDS             1222..>2530
                     /locus_tag="VF589_03005"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8256577.1"
                     /translation="MHLLRRCLLALFLLVSVPASAAGDADWLYRGSDIERDPNWIFGT
                     LPNGLRYAVRQNKLPVGQVSIRLRIDAGSLHEADQERGWAHFVEHMAFRGTRDFADRE
                     ARHVWQKLGASFGSDTNAFTSPTQTVYQLDLPNADASEIDLSLDVIAGMADNALFDPA
                     TVEAEKKVILAEKGRRPELTNRYIETSWPLFYAGLKIAERDTIGTEPTLRAATAAGLR
                     GFYERWYRPDRATLIIVGDADPKALEAIVAKHFSRWQASGPKPAEPDFGTISRTLQRT
                     AALAYPGTPHSASLLWLRPYQDLPDTKAHEQADMARALAARIINRRLEAKARGDAAFV
                     SAGLGESRQAGIADMTQLSLTARGGRWREAMTESFAILADALRAPPSETEISREIQNL
                     RTAYTSAVEGVGTVKSPQWAQQLVGAIDADSVVASPQTSLALLE"
BASE COUNT          415 a          907 c          846 g          362 t
ORIGIN      
        1 ctggcgggcg aggacgaggc gccagcgcgc gcggccgacg ccggggacgg ggagcggcga
       61 cgagggcgcg caggcgattc gccagcgtgt gaccgcggac gaaggggcag cgagggggtc
      121 ctcgccgttc ctgcgccagc gcttcagcat cagggccatc gccccgccct cctcggcggc
      181 gagctggagg cggcgggcgg cggccatggg cacgcgcgcc gcctcgccca ccaccgccgc
      241 gaggccgccg tggcgaagcc cttcctccat caccgcaagc acatcctcgt cgcggccgca
      301 ttcggcgtag aggatgcggt ccggcggcag ccccgccaaa gcaaggccgg gcgcgaaaag
      361 gtcgcggcgg gagagcgccc agagcacggt gcccggccgg cgggcggcga tgccggcgac
      421 gaacaaggtc gcggcggcat cgtcgctgag atcaggccgc gcgccggtta tttcgtggag
      481 tgcggccaaa gcgaggccgc cgccctccag ccggcgatcg attgcatcga cgccgaaggg
      541 caggcattca cgcccggctt tcgctgtcga cgattcgatc gcccgcacct cggcccgcaa
      601 ggcggcaagg cgctcggcac gggcggggga caagggcatg acgactccag agactcactc
      661 gttcccttta tgttcctacc gcttctacag agtcaatgga gagtctgtgt gtcagcccgt
      721 ggttgacatc tcggctatgc cgttgatgct tcgggtaatt ggcggatcac gttcagctgg
      781 gacggcgagg atgccgtcga tgtcgatctg gaggattgtc actgatgccg aacccactgc
      841 tgaagggtct gaggccggtc cacccaggtg agttgctgcg cgaggacgtc ctgccggcgc
      901 tcggccgcac caaggcggag atcgcccgcc tgctcggcat ttcgcggcag acgctctacg
      961 acatcctcaa cgaacgccag cccgtcaccc ccgctatggc gcaccgcctc gccaagctgc
     1021 tcggcggctc ccgcgaggcg tggctcaaca tgcagcgcaa ttgcgacctc gccgccgccg
     1081 aacgggaatt ggcggatgcg cttgccgcca ttcctacgct aaaagcggca tgaagcagcc
     1141 gctccgccga ccgccgatgt tcaggcgcga gacaggcggc ttgtgaagaa aagccccctc
     1201 ccccacttaa tcacgagatc catgcacctc ctccgccgct gcctcctcgc cctgttcctc
     1261 ctggtcagcg tccccgcttc ggcggcgggc gatgccgatt ggctgtatcg cgggagcgat
     1321 atcgagcgcg acccgaactg gattttcggg acgctgccca acggcctgcg ctacgcggtg
     1381 cgacagaata aattgccggt cgggcaggtc tcgatccggc tgcggatcga cgccggatcg
     1441 ctgcacgagg ccgaccagga gcgcggctgg gcgcatttcg tcgagcatat ggcgttccgc
     1501 ggcacgcgcg acttcgccga ccgcgaggcg cggcacgtct ggcagaagct cggcgccagc
     1561 ttcggcagcg acaccaacgc cttcaccagc ccgacccaga cggtctacca gctcgacctt
     1621 cccaatgccg atgcgagcga gatcgacctc agcctcgacg tcatcgccgg catggccgac
     1681 aatgccttgt tcgacccggc cacggtcgaa gccgagaaga aggtgatcct cgccgaaaag
     1741 ggccgccggc cggagctcac caaccgctat atcgagacca gctggccgct cttctatgcc
     1801 ggcctcaaga tcgccgagcg cgacacgatc ggcaccgagc cgaccttgcg ggcggccacc
     1861 gccgcgggcc tgcgcggctt ttacgagcgc tggtaccggc ccgaccgggc gacattgatc
     1921 atcgtcggcg atgccgaccc caaggcgctg gaagcgatag tggccaagca tttcagccgc
     1981 tggcaggcga gcggccccaa gcccgccgaa cccgatttcg gcaccatctc ccggaccctg
     2041 cagcgcaccg ccgcgctcgc ttatccgggg acgccgcatt cggcctccct gctgtggctg
     2101 cgcccctatc aggacctgcc cgacaccaag gcgcacgagc aggccgacat ggcgcgcgcg
     2161 ctcgccgcgc ggatcatcaa ccgccggctc gaggccaagg cgcgcggcga cgccgccttc
     2221 gtcagcgccg gcctcggcga atcgcgccag gccggcatcg ccgacatgac ccagctttcg
     2281 ctgaccgcgc gcggcggcag gtggcgcgag gcgatgaccg agagcttcgc gatcctcgcc
     2341 gacgccctgc gcgcgccgcc gagcgagacc gagatttccc gcgagatcca gaatctgcgc
     2401 accgcctaca cctcggcggt ggagggcgtg ggcacggtga agtcgccgca atgggcgcag
     2461 caactggtcg gcgcgatcga tgcggacagt gtcgtcgcgt ctccccagac ctcgctcgcc
     2521 ttgctcgagg
//
LOCUS       DASVRK010000092         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_451290, whole genome shotgun sequence.
ACCESSION   DASVRK010000092 DASVRK010000000
VERSION     DASVRK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451290"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..419
                     /locus_tag="VF589_03010"
     CDS             258..419
                     /locus_tag="VF589_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256578.1"
                     /translation="MTRGQTERFISKQEETQELLRESIEQSKELIAQSERLIDAQREH
                     PVSRPFIAR"
     gene            499..636
                     /locus_tag="VF589_03015"
                     /pseudo
     CDS             499..636
                     /locus_tag="VF589_03015"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            686..1684
                     /locus_tag="VF589_03020"
     CDS             686..1684
                     /locus_tag="VF589_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8256579.1"
                     /translation="MPDYVRDLRQPAGDPGTFFVDPELAPPRLTGRQALEAAAAAPSL
                     AAHLSGVRRMNPLYEELRRGLAAWRAGGGSDPAQERLIRASLERARAIPADPGRRFIL
                     VDAAGARLWLYEDGRVVDTMRAIVGKPAQQTPAMAGLIRFAVLNPYWNLPQDLVPGRI
                     ARHVLSQGPDYLRRERMEILSDYTAGARRLDPSEVDWPAVAAGTERLRVRQLPGAGNM
                     MGKVKFMMPNRLGIYLHDTPDKSSFGRADRRLSSGCVRVEDAERLSRWLFGGRAVQAA
                     GAAAEQRVDLPAPVPVYITYLTAAPAEGGGIAFQADVYGRDPEWLARLDAKAAPAA"
BASE COUNT          312 a          751 c          670 g          312 t
ORIGIN      
        1 aatctcatta tgccggcccc accgcggctt atgagctgac cctcagccgc aaggtcgagg
       61 ccacgctcgg cctcgcttat ctccgccgca tcgacagcga ccccggcctc agcgacgtga
      121 tccagctctc cggctcaatc cgattctgaa caggggctgg gcgccgcccc cggcgggcca
      181 agaaagagtc aatttgtgga aaccattgcc attaatctac gttaataggc ttcatccaac
      241 cccctgaaat ggttttcatg acgcgcggcc aaaccgaaag attcatctcc aaacaggaag
      301 agacccagga gctgctccgg gagagtatcg agcagagcaa ggaactcatc gcccagtcgg
      361 agcgcctgat cgacgcccag cgcgagcacc cggtcagccg acccttcatc gcccgctgac
      421 ctgatcgcga gggcgccccg ctcgccggcg cgatggcccc acgcggctgc gctctgattc
      481 tgtcaatcgt tccggccact ctgggtcgag ggcgctgccc tgcggcctga agcgcgaacc
      541 ctcatggccc tgatcgcgcg cgcgcccgag gatgggctcg atccacaacg ctataatctc
      601 cccgcgctcg tggccgcgct agcggcggcg gagtaaggcg ggtcggccgc gcttgcccga
      661 gccgaggcgc tgctttccag ggtttatgcc cgattatgtg cgcgatctcc gccagccggc
      721 gggcgacccg ggcaccttct tcgtcgatcc ggaactggcg ccgccgcggc ttacgggccg
      781 ccaagcgctc gaagcggcgg ccgctgcgcc ctcgctcgcg gcccaccttt cgggcgtaag
      841 gcggatgaac ccgctttatg aggagctgcg ccgcggcctc gcggcctggc gcgcgggcgg
      901 cggctccgat ccggcgcagg agcggctgat ccgcgccagt ctcgaacgcg cccgcgcgat
      961 cccggccgat cccggccggc gcttcatcct ggtcgacgcc gccggcgcgc gactgtggct
     1021 ctacgaggat ggccgcgtcg tcgacacgat gcgggcgatc gtcggcaagc cggcacagca
     1081 gacccccgcc atggccgggc tgatccgctt tgccgtgttg aacccttatt ggaacctgcc
     1141 gcaggatctg gtgccggggc ggatcgcccg ccatgtcctg agccagggcc ccgattatct
     1201 ccggcgggag cggatggaga tactctcgga ctatacggcc ggcgcgcgcc ggctggaccc
     1261 gtcggaggtc gactggcccg cggtcgcggc cggcaccgag cggctgcgcg tccgccagct
     1321 gcccggcgcc ggcaacatga tggggaaggt caagttcatg atgccgaacc ggctcggcat
     1381 ctacctccac gacacgcccg acaaatcgag cttcggccgc gccgaccgcc ggctcagttc
     1441 gggctgcgtc agggtcgagg atgccgagcg gctgtcgcgc tggctgttcg gcggccgcgc
     1501 cgttcaggcg gccggcgcgg cggcggagca gcgcgtcgac ctccccgccc cggtgccggt
     1561 ctatatcacc tatctcaccg ccgctccggc cgaaggcggc ggcatcgcct tccaggccga
     1621 cgtttatggc cgcgaccccg aatggctcgc ccggctcgac gccaaggccg cacccgccgc
     1681 ctgattcaac gcgccggcgc cgaactacca cgcttgctct cacgctcgct cacactggcc
     1741 ccgctttacc cggctgcctg ccgccgaccc gtccgcattg accccggcac cggcagcgac
     1801 atcgtcgtcc agggcttgtc gccgtcgatc ccagacatgg ccatcgaaca tcatttcgga
     1861 tgatccgagg ggcggggccg cgccgcgcgc gccgcccgcc gtgcagggcg aagcgcggaa
     1921 gatcacacta aggtcacacc aacaaaggct cggcgccggg cctggtttcc ccgtgttcgc
     1981 gccgcggcat tggcggcgag gggaatggga gaaaccgggc ggtagcggcc gacggccggt
     2041 gcccg
//
LOCUS       DASVRK010000093         5475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_388652, whole genome shotgun sequence.
ACCESSION   DASVRK010000093 DASVRK010000000
VERSION     DASVRK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388652"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VF589_03025"
     CDS             <1..623
                     /locus_tag="VF589_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8256580.1"
                     /translation="VAQAQLGQTRARMGRLDVRAPAAGLVLDRMVEPGQIVSSGSGAL
                     FRIAMGGQMELLARMSQEDLAAVRPGIPATVTPVGSSRSFTGQVWQVAPVIDPQSRQG
                     EARIALTYDPLLRPGGFASAQIISGQVDAPLLPEAAVQSDEKGSYVYVIDNKDTVTRR
                     DVQVGQVTDAGVTIVQGLGGNERVVQSAGAFLNPGDKVRPVRASAR"
     gene            677..3763
                     /locus_tag="VF589_03030"
     CDS             677..3763
                     /locus_tag="VF589_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8256581.1"
                     /translation="MSFRNISAWAIRNPVVPLVMFFALTLAGLVSFMRMDINNNPDIS
                     FPAVTVTVVQPGAAPAELETQVTQRVEAAVRAISGVDEITSVAREGESFTIVQFAIGT
                     PVDRAVNDVRNAVDQVRSQLPEGILEPQVTRMDIDGGPIAYFSAEATDMTLEELSWYV
                     DNTVAKRLLGIEGMAAVQRGGGVSREIRILLDPAKLQGQGLTAAQVNQQLRQVNMNAA
                     GGRAEIAGSEQSVRVLGNAADAFALGQSQISVPGGRTVKLADIADVRDTYAEQRNLAM
                     MNDRQVTSFSMAKAKGSSDVTVYDAAMKELEALNKENPKVTFKQLYTSVDYTKDQYNS
                     AMIAMVEGAVLAVIVVFIFLRDWRATIISALAIPLSAIPTFWFMDLMGFTLNSISLLA
                     LSLVAGVLVDDAIVEIENIVRHMRMGKSAYHASIDAADEIGLAVLATTMAIVAVFLPV
                     ALMPGISGQFFIQFGMTVVIAVLLSLAVARLITPMIAAYFLKSHGQATHGEGWLMDVY
                     MKILKWSVLHRWKTVILFGGGAFVATIYAFYTLPMTFQPTTNTDYSQVKVEMVPGTTV
                     QQTREATQRVAQMLSADTAVVKDAFADIRPTEATIYLTLKEERPITSVEWERKTAPIF
                     EQVADARVNFQSQSGGGFGRDIIIMFGSDNPELLEKTANQLTTEMAGLKELRAPRVVG
                     DLQRPEIIVKPHMDLAADMGVTTAALSQTIRIATMGDIDQNSAKFSLADRQIPIRVSL
                     TEESRRNLSTIQNLPVPTADGGSVPLKVVADVEFGAGPTQIRRYNQIRRIVVGADLAP
                     ELVTSEAMAKINELPTMKNLPQGVRKLDIGDSKWQAELLMNFVIAVISGVLLVFAVLV
                     LLYKRIMPPFVNMGSLLLAPLGGALALHLTGQPISMPVFIGLLMLLGIVAKNSILLID
                     FALEEMGKGVPKLEAIIDAGHKRAQPIVMTTVAMVAGMVPTALSLSGDGAWRAPMGVT
                     VIGGLILSTLLTLLIVPAGFSLADSFEKWLGPKFGRLLTYRGQQDHGPEPAIQPAE"
     gene            3796..5061
                     /locus_tag="VF589_03035"
     CDS             3796..5061
                     /locus_tag="VF589_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX8256582.1"
                     /translation="MSLLERTGLSRFNPAQGGVRGMRIVATGMLVVMAAIFLVANELD
                     ERWVAWGFVKAFAEAAMVGGLADWFAVTALFRHPLGLPIPHTAIIPRNKDRIGDTLAQ
                     FLRDNFLTPSVVARRMVGIDVAGAAGRFLVNPPGGGRLREGASRLIADILEALDQERL
                     GGMVKSAVAGRLRALEISPMLGQTLDAAITEDRHIPLLDSLVTWIGRTLDANEDMIRA
                     MVHQRAGWVVRLAGLDAKLADAIVDGLRKLTVDMAVDPNHPLRAKAEEGLANLAWDLQ
                     HDPDMRAKVEALKAEIVGNKAVSAWIDGLWEKARAGLLRAARDPEATMAGKFGEAIRQ
                     LGSTLQEDPHLRTTINQFARRAVVGGVASYGTGIVALVSDTIRGWDAQTVTNRLEGAV
                     GRDLQYIRINGTLVGGLVGLIIHVVEVVT"
     gene            complement(5092..>5475)
                     /locus_tag="VF589_03040"
     CDS             complement(5092..>5475)
                     /locus_tag="VF589_03040"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951706.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8256583.1"
                     /translation="SISTAPFPAFPTDMQAQFMSMLALAKGTSLLTETIFENRYMHVP
                     ELARMGADIDVRGRSAVVRGVEKLHGAPVMATDLRASMSLILAGLAAEGETQVGRVYH
                     LDRGYERLEEKLQAVGAEIERVSAG"
BASE COUNT          914 a         1926 c         1780 g          855 t
ORIGIN      
        1 gggtggccca ggcgcagctc ggccagaccc gcgcccggat gggccggctc gacgtgcgcg
       61 cgcccgccgc cggcctggtg ctcgatcgca tggtcgagcc ggggcagatc gtgagctcgg
      121 gctcgggcgc cttgttccgc atcgccatgg gcggccagat ggagctgctc gcgcgcatgt
      181 cgcaggaaga cctcgcggcg gtccggcccg gcattccggc gacggtgacg ccggtcggat
      241 cgagccgcag cttcaccggt caagtgtggc aggttgcgcc ggtcatcgat ccgcaaagcc
      301 gccagggcga ggcgcggatc gccttgacct acgatccgct gctgcggccg ggcggcttcg
      361 ccagcgcgca gatcatctcg ggccaggtcg acgcgccttt gctccccgaa gcggcggtgc
      421 agagcgacga aaagggcagc tacgtctacg tgatcgacaa taaggacacc gtgacgcggc
      481 gcgacgtgca ggtcgggcaa gtgaccgacg ccggcgtcac catcgtccag ggcctcggcg
      541 gcaacgagcg ggtggtccag tcggcgggcg ccttcctcaa cccgggcgac aaggtaaggc
      601 cggtgcgggc gagcgcccgc tgagcccggc gccggcgcgc gggcgggatg agcatggaca
      661 ggattgaggc agtatcatga gttttcgcaa catctcggcc tgggcgatcc gcaatccggt
      721 cgtgccgctc gtcatgttct tcgcgctgac gctggccggc ctggtcagct tcatgcgcat
      781 ggacatcaac aacaatcccg acatcagctt ccccgccgtc accgtaacgg tggtgcagcc
      841 gggcgccgcg ccggccgagc tcgagaccca ggtcacccag cgcgtcgagg ccgcggtccg
      901 cgcgatcagc ggcgtcgacg agatcacctc ggtggcgcgc gagggcgaaa gcttcaccat
      961 cgtccagttc gcgatcggca cgcccgtcga ccgcgccgtc aacgacgtcc gcaacgccgt
     1021 cgaccaggtc cgcagccagc tacccgaggg catcctcgag ccgcaagtca cgcgcatgga
     1081 cattgacggc ggcccgatcg cctatttcag cgccgaagcc accgacatga cgctcgagga
     1141 attgagctgg tacgtcgaca atacggtcgc caagcggctg ctcggcatcg agggcatggc
     1201 cgcagtgcag cgcggcggcg gcgtcagccg cgaaatccgt atcctgctcg atccggccaa
     1261 gctgcaggga cagggcctca ctgccgcgca ggtcaatcag cagcttcgcc aggtgaacat
     1321 gaacgccgcc ggcggccgcg ccgaaatcgc cggatccgag cagtcggtgc gggtgctggg
     1381 caacgccgcc gacgccttcg cgctcggcca gtcgcagata tcggttcccg gcggccgcac
     1441 cgtgaagctc gccgacattg ccgacgtgcg cgacacctat gccgagcagc gcaacctggc
     1501 gatgatgaac gaccggcagg tgaccagctt ctcgatggcc aaggccaagg gttcgtccga
     1561 cgtcaccgtc tacgacgccg cgatgaagga gctcgaagcg ctcaacaagg aaaaccccaa
     1621 ggtcaccttc aagcagctct atacgagcgt cgactacacc aaggaccaat ataattcggc
     1681 gatgatcgcc atggtcgagg gcgccgtgct ggcggtcatc gtcgtcttca tcttccttcg
     1741 cgactggcgc gcgacgatca tctcggcgct ggcgatcccg ctctcggcga tcccgacctt
     1801 ctggttcatg gacctgatgg gcttcacgct gaacagcatc tcgctgctcg cattgagcct
     1861 ggtcgcgggc gtgctcgtcg acgacgccat cgtcgagatc gagaatatcg tgcgccacat
     1921 gcggatgggc aaatccgcct atcatgcctc gatcgacgcc gccgacgaga tcggcctcgc
     1981 cgtgctcgcc accacgatgg cgatcgtcgc cgtgttcctg ccggtggcgc tgatgcccgg
     2041 catttccggc cagttcttca tccagttcgg catgaccgtg gtgatcgcgg tgctgctgag
     2101 cctcgcggtc gcgcgcctga tcacgccgat gatcgccgct tatttcctga agtcgcacgg
     2161 ccaggccacg cacggcgaag gctggctgat ggacgtctac atgaagatcc tgaaatggtc
     2221 ggtgctgcac cgctggaaga cggtgatcct gttcggcggc ggcgccttcg tggcgaccat
     2281 ctacgccttc tacacgctgc cgatgacgtt ccagccgacc accaacaccg actatagcca
     2341 ggtcaaggtc gagatggtgc ccggcaccac cgtccaacag acccgcgagg cgacccagcg
     2401 cgtcgcccag atgctctccg ccgacaccgc ggtggtgaag gacgcgttcg cggacatccg
     2461 cccgaccgaa gcgaccatct acctgacgct gaaggaggag cggccgatca ccagcgtcga
     2521 gtgggagcgc aagaccgcgc cgatcttcga gcaggtcgcc gatgcccgcg tgaacttcca
     2581 gtcgcagtcc ggcggcggct tcgggcgcga catcatcatc atgttcggca gcgacaatcc
     2641 ggagctgctg gagaagaccg ccaaccagct gaccaccgaa atggccggcc tcaaggagct
     2701 gcgcgccccg cgcgtggtcg gcgacctcca gcgccccgag atcatcgtca agccgcacat
     2761 ggacctcgcc gccgacatgg gcgtcaccac cgcggccttg agccagacca tccgcatcgc
     2821 gacgatgggc gacatcgacc agaacagcgc caaattctcg ctcgccgacc gccagatccc
     2881 gatccgcgtg tcgctgaccg aggaatcgcg ccgcaacctc tcgaccatcc agaaccttcc
     2941 ggtccccacc gccgacggcg gctcggtacc gctgaaggtg gtcgccgacg tcgaattcgg
     3001 cgccggcccg acccagatcc gccgctacaa ccagatccgg cgcatcgtcg tcggcgccga
     3061 tctcgcgccc gagctcgtca ccagcgaggc aatggccaag atcaacgagc tgccgacgat
     3121 gaagaacctg ccgcagggcg tccgcaagct cgatatcggc gattccaaat ggcaggccga
     3181 attgctgatg aacttcgtga tcgcggtgat ctcgggcgtg ctgctggtgt tcgcggtgct
     3241 ggtgctgctc tacaagcgga tcatgccgcc gttcgtgaac atgggctcgc tgctgctggc
     3301 gccgctcggc ggcgcgctcg cgctgcacct gaccggccag ccgatctcga tgccggtgtt
     3361 catcggcctg ctgatgctgc tcggcatcgt cgccaagaac tcgatcctgc tgattgactt
     3421 cgcgctcgag gagatgggca agggcgtgcc caagctcgag gcgatcatcg atgccggcca
     3481 caagcgcgcc cagccgatcg tgatgaccac cgtcgcaatg gtcgccggca tggtgccgac
     3541 cgcattgtcg ctgtccggcg acggcgcctg gcgggcgccg atgggcgtca ccgtgatcgg
     3601 cggcctcatc ctgtcgaccc tgctgaccct gctgatcgtg ccggccggct tcagcctcgc
     3661 cgacagcttc gagaagtggc tcggccccaa attcgggcgg ctgctcacct atcgcggaca
     3721 gcaggatcac gggccggagc cggcgatcca gccggccgag tgaaccaaaa agccgatccg
     3781 tctattgagc aggccatgag cctgctcgaa cggactggtc taagccgatt caaccccgcg
     3841 cagggcggcg tccgcggcat gcgcatcgtg gcgactggga tgctggtggt gatggccgcc
     3901 atcttcctgg tcgccaacga gctcgacgaa cgctgggtgg cttggggctt cgtcaaggcc
     3961 ttcgccgaag ccgccatggt tggcggcctc gccgactggt tcgccgtcac cgccttgttc
     4021 cggcacccgc tcggcctgcc gatcccgcac accgccatca tcccgcgcaa caaggaccgg
     4081 atcggcgaca cgctcgccca gttcctgcgc gacaatttcc tcaccccctc ggtggtggcg
     4141 cggcggatgg tggggatcga cgtcgccggc gcggccggcc gcttcctcgt caacccgccc
     4201 ggcggcggcc ggctgcgcga aggcgcctcg cggctgatcg cggacatcct cgaagcgctc
     4261 gaccaggagc ggctcggcgg catggtcaag tccgccgtcg ccggccggct gcgcgcgctc
     4321 gaaatctcgc ccatgctcgg ccagacgctc gacgcggcga tcaccgagga ccgccacatc
     4381 cccttgctcg acagcctcgt cacctggatc ggccgcaccc tcgacgccaa cgaggacatg
     4441 atccgcgcca tggtccacca gcgcgccggc tgggtcgtcc gcctcgccgg cctggacgcc
     4501 aagctcgccg acgcgatcgt cgacggcctg cgcaagctca ccgtcgacat ggcggtcgat
     4561 cccaatcatc cgctgcgcgc caaggcggag gaggggctcg ccaacctcgc ctgggacctc
     4621 cagcacgatc ccgatatgcg cgccaaggtc gaggcgctga aggccgagat cgtcggcaac
     4681 aaggcggtca gcgcctggat cgacggcctg tgggaaaaag cccgcgccgg cctcctccgc
     4741 gcggcgcgcg atcccgaggc cacgatggcc ggcaagttcg gcgaggcgat ccgccaattg
     4801 ggctcgactt tgcaggagga tccgcacctg cgcaccacca ttaatcagtt cgcccgccgc
     4861 gccgtcgtcg gcggcgtcgc cagctacggc accggcatcg tcgcgctcgt gtcggacacg
     4921 atccgcggct gggatgctca gaccgtcact aaccgcctcg aaggcgccgt cggccgcgac
     4981 ctccaatata tccgcatcaa cggcacgctg gtgggcgggt tggtcggcct gatcatccac
     5041 gtggtggaag tggtgactta aggggcccgc gccgtcccgt cactccagcg gtcaccccgc
     5101 gctgacccgc tcgatctcgg cgcccaccgc ctgcaacttc tcctcgagcc gctcataccc
     5161 ccggtcgaga tgatagaccc gccccacctg ggtctcgcct tccgcggcga ggccggccag
     5221 gatcaggctc atcgacgcgc gcaggtcggt cgccatcacc ggggcgccgt gcagcttctc
     5281 gacgccgcgc accacggccg agcggccgcg cacgtcgatg tcggcgccca tgcgggcgag
     5341 ttcgggaacg tgcatgtagc ggttctcgaa aatggtctcg gtgagcaggc tggtgccctt
     5401 ggcgagcgcg agcatcgaca tgaattgcgc ctgcatgtcg gtcgggaaag ccgggaaggg
     5461 cgcggtcgag atcga
//
LOCUS       DASVRK010000094         9243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_326891, whole genome shotgun sequence.
ACCESSION   DASVRK010000094 DASVRK010000000
VERSION     DASVRK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326891"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VF589_03045"
     CDS             <1..897
                     /locus_tag="VF589_03045"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8256584.1"
                     /translation="MMAHDALAAGTADIIVAGGMESMTNAPFLMQKHRAGARIGHDTV
                     YDHMMLDGLEDAYDKGRAMGTFAEDAAREYQFTREAQDEYAIESLRRATEAIRSGAID
                     RETVSVTVKTRKGEEAVACDEQPGKSNPEKIPGLKPAFAKDGTITAANASSISDGAAA
                     LVITRQSIADRLGHKPVARIVAHAAHAQAPNLFTSAPVPAVRKALEKAGWSVGDVDLW
                     EVNEAFACVAMIAMRDLGIPREALNVHGGATALGHPIGASGARLLATLLSALETHGQR
                     RGLATLCIGGGEATAMAVELMD"
     gene            complement(985..1260)
                     /locus_tag="VF589_03050"
     CDS             complement(985..1260)
                     /locus_tag="VF589_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8256585.1"
                     /translation="MREIVWTDQAVGHLEAIVTYISAVNPAAAERLAQRLIAVADSLV
                     EFPDRGRDAGQGRREMTTVWPYILRYRVEGETVLILRIRHGAREEDQ"
     gene            complement(1479..1622)
                     /locus_tag="VF589_03055"
                     /pseudo
     CDS             complement(1479..1622)
                     /locus_tag="VF589_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
     gene            1677..2336
                     /locus_tag="VF589_03060"
     CDS             1677..2336
                     /locus_tag="VF589_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256586.1"
                     /translation="MSAIPLLLLAAAQPAAPPPGAQLPPKAVSCIIKAGGRRVVDLLV
                     TEPATPAEAAAFQRLGTALKRCGALSAAGYAAEGTAIRAALSRTLVTRVAQRVVTGPN
                     SNPNFSPNLFTVQRGYDHAGVMQGPYRLAKCMTSVNLMGVAKVVATAPGTPAEREAMQ
                     AVAPTLASCVDRNQQVALSTRTLRMAIDIVYAQRTFGLLRTMPLPGRRVVPPPPVGSE
                     K"
     gene            complement(2749..3666)
                     /locus_tag="VF589_03065"
     CDS             complement(2749..3666)
                     /locus_tag="VF589_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX8256587.1"
                     /translation="MIAIVHHPDYVAPARAGSTYAWNKNGLVRDLLLESGRGIDWIEP
                     EAMPRAWLEAVHDPSYVAEVIEARVPAAKERRIGFAVTEAVARRAVRVPGGTWRAAIE
                     AIGRGHAANIAGGSHHALHDSGAGYCVFNDLAVAAVRLVEEGRVERVLIVDCDVHQGD
                     GTAALTAGRADIATYSIHADKNFPVRKARSTVDVALADGVGDDEYLDRLEATLTPLVE
                     RFRPALILYQAGVDPFSGDRLGRLALTQGGLDARDALVARLARERGVPLASTLGGGYG
                     ADVLEVARRHVRSIFALADAFGEKALPAR"
     gene            3725..4678
                     /locus_tag="VF589_03070"
     CDS             3725..4678
                     /locus_tag="VF589_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256588.1"
                     /translation="MSDPVVRPDVRTFLDYLNALPGPRSHQVGPIEARAMMHASRHIA
                     DAPVGELAVIRDLAAPGPAGEILLRLYDPREERDPGPVMLFLHGGGFVLGDLDTHAPF
                     CAEMARLMDLPLVAVDYRLAPEHPWPAAPEDAIAAARWVAESPAALGRAVTGLVAIGD
                     SAGGNLAIVTSLALRDEPAAVPVLAQWPIYPAADPAKGYPSFTDFGEGYLLTREGMHW
                     FDRCYAADPNDWRYAPLVKSQRGMPPTLVVTAGLDPIRDQGRAYAAACVAAGVPTIFR
                     EAEGNIHGFINLRKALPSSEADIRGCAVALHLMLTEAIDGR"
     gene            4668..5144
                     /locus_tag="VF589_03075"
     CDS             4668..5144
                     /locus_tag="VF589_03075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX8256589.1"
                     /translation="MVDKETLPYRPAAAVMLLNRDGKVFVAQRLDSTLEAWQMPQGGL
                     DEGEEPLAGALRELEEETGIRPEHVELIAAAPAEFHYDLPDDLIGKMWKGKWRGQRQS
                     WFLMRFTGTDADVNIATADPEFRAWKWADPESLPAMIVPFKKRLYEDVLAAFRRYL"
     gene            complement(5158..5242)
                     /locus_tag="VF589_03080"
     tRNA            complement(5158..5242)
                     /locus_tag="VF589_03080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5206..5208),aa:Leu,seq:caa)
     gene            5622..6044
                     /locus_tag="VF589_03085"
     CDS             5622..6044
                     /locus_tag="VF589_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256590.1"
                     /translation="MSDSAPDDPLHFDPVPSASTRHDGWTPARQRRFIAVLRDSGKVA
                     AAARAAGKSRKSAYDLLKRAGPDSGFARAWDTAQGEQRRLRAADAAAAIERALHGEEV
                     GYYYGGLERGVRRVYDDRLLIAALRAAGRAPGLARRRK"
     gene            6177..6449
                     /locus_tag="VF589_03090"
     CDS             6177..6449
                     /locus_tag="VF589_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HEX8256591.1"
                     /translation="MAGGWSRDGAVQDQIDDTVKDAVLAARARLAAAAGAESETHCIE
                     CGEEIPEGRRRALPGARTCVACQSARDRRSPAAGINRRGSKDSQLR"
     gene            complement(6452..6886)
                     /locus_tag="VF589_03095"
     CDS             complement(6452..6886)
                     /locus_tag="VF589_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256592.1"
                     /translation="MNGSVKTPVHLWIVGALAAVWNGFGALDYTMSQTRNPGWMAQMT
                     EGQRAWLEAAPAWADAAWAFGVWGGLAGSLLLLARSRHAVTAFILSIAGLAVNTLFQF
                     ASPMPSGHMDGGAAIALHVAIWAAATALLLYALRMRARGALR"
     gene            7191..7484
                     /locus_tag="VF589_03100"
     CDS             7191..7484
                     /locus_tag="VF589_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX8256593.1"
                     /translation="MQSYQPYLFNRSDTFFGVCEGLGEDFGFNANYLRVALALAIFWN
                     PVMVAGGYAGAGVIVALSRWLVPNPRPAPAVEAAETPMPANTTEPAEALPLAA"
     gene            complement(7523..8014)
                     /locus_tag="VF589_03105"
     CDS             complement(7523..8014)
                     /locus_tag="VF589_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX8256594.1"
                     /translation="MLAAEPAAQQRVVGDSVTVRVLQAARAAMLHALRSQATAAPILS
                     DSAALLDYLMLDQGHAPVERLRVLYLNGQNALLGETVTDGTVNEAAFYPREILKRALE
                     LGATALILVHNHPSGDLSPSQSDLQITRAIAGVGRTLRVVVHDHLILSRHGWTSFKAL
                     GLL"
     gene            complement(8041..8550)
                     /locus_tag="VF589_03110"
     CDS             complement(8041..8550)
                     /locus_tag="VF589_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256595.1"
                     /translation="MLFYFFCLFAIVAYALWRGGKWEKLGALTLLAGSILTLIAVSAA
                     SRRFADVETGILLVDAAVLAAFLAIALRSDRYWPLWTAALQLIGLLGHFAKLADLEMP
                     RNGYGFLQAFWSYPMMLTILIGTWNQHRAQHRGQQRAKHWGSRAAGPTSPRSWRGWAR
                     RGRRPPPER"
     gene            complement(8616..8957)
                     /locus_tag="VF589_03115"
     CDS             complement(8616..8957)
                     /locus_tag="VF589_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256596.1"
                     /translation="MRHHLIAKARKIAADVRNSEDQYDLALADTARLVASLLDARRTS
                     GVPARTGREALDRALQAISHGAKARESLLDMHAELARLDLRELAVGDVVECPEDWAAN
                     GLQIVASRDAA"
     gene            complement(9019..>9243)
                     /locus_tag="VF589_03120"
     CDS             complement(9019..>9243)
                     /locus_tag="VF589_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256597.1"
                     /translation="VREEQAVFAAAFPYPAARMLPFPRHQGERNELPAATRLLWIGGI
                     AIGVVLALAALIATSWGVVRMVGQAARTFS"
BASE COUNT         1457 a         3395 c         3092 g         1299 t
ORIGIN      
        1 atgatggcgc acgacgccct ggccgccggc accgcggaca ttatcgtggc ggggggcatg
       61 gagagcatga ccaacgcgcc gttcctgatg cagaagcacc gcgccggcgc ccgcatcggc
      121 cacgacacgg tctacgacca catgatgctc gacggcctcg aagacgccta cgacaagggc
      181 cgcgcgatgg gcaccttcgc cgaggacgcc gcccgcgaat accagttcac ccgcgaggcg
      241 caggacgaat acgccatcga gtccctgcgc cgcgccaccg aggcaatccg ctccggcgcc
      301 atcgaccgcg aaaccgtctc cgtcaccgtc aagacccgca agggcgagga agccgtcgcc
      361 tgcgacgagc agcccggcaa gtccaacccg gagaagatcc cgggcctgaa gcccgccttc
      421 gccaaggacg gcaccatcac cgccgccaac gcgtcgagca tctcggacgg cgccgccgcc
      481 ctcgtcatca cccgccagtc gatcgccgac cgcctcggcc acaagcccgt ggcccgcatc
      541 gtcgcccacg ccgcccacgc ccaagcgccc aacctcttca ccagcgcccc cgtcccggcg
      601 gtgcgcaagg cgctcgagaa agccggctgg agcgtcggcg acgtcgacct gtgggaagtc
      661 aacgaggcct tcgcctgcgt cgccatgatc gccatgcgcg acctcggcat cccacgcgag
      721 gccctcaacg tccacggcgg cgccaccgcc ctcggccacc cgatcggagc cagcggcgcc
      781 cgcctcctcg ccaccctcct cagcgcgctg gaaacccacg gccaacgccg cggcttggcg
      841 acgctgtgca tcggcggcgg cgaggcgacc gcgatggcgg tggagctgat ggactgaacg
      901 cgacggagct ggctgatcaa caactgcttg cagcttgcgt cggcgcaggc gcggccgccg
      961 tgcaggccat tggggcgatt ggagctattg gtcctcctcc cgcgccccat ggcggatgcg
     1021 caggatcagc acggtttcgc cttctacgcg atagcgaagg atgtacggcc acacggtcgt
     1081 catctcccgc cgcccctggc ctgcgtcgcg cccgcggtcc ggaaactcta ccaggctgtc
     1141 cgcaacggcg atcaagcgct gcgccagacg ctccgccgct gcgggattca cagcggaaat
     1201 gtaggtgacg atcgcctcca gatggccgac cgcctggtcg gtccagacga tctcacgcaa
     1261 atcagtcccc gacgcgcggg cgcggcagcg gcttgtgggt cccccaggac gcaagccacc
     1321 gtcgcaccgc tccgtggcta atcagccgac cggcggcgac gtccgcctcc gcgcgagctt
     1381 cagacttcgc ctccgcctcc gcgtcgggcg agtcgaacag gcctttctcg gtacccatgc
     1441 cgatagcata gcagcttcgg caggccggga aaatgcgctc aataacgata ccggcactgc
     1501 gccacctcca gcgcctgggc ctcggccctc cccgtcaggc ggtagaccag gcggtgctcg
     1561 cgatcgatgc ggcgggacca ccagccgctg aggttgccgc ccagcggctc ggtcttgccg
     1621 gtgccgcgga aggggcaccc ttcgcgatcg ccggcttatc ccctaagctc aggcgcatgt
     1681 ccgcgattcc gcttctcctg ctcgcagcgg cccagcccgc ggccccgccg cccggcgcgc
     1741 agctgccgcc caaggcggtg agctgcatca tcaaggcggg cggccggcgc gtcgtcgatc
     1801 tgctggtgac cgagcccgcc acgccggccg aggcggccgc attccagcgg ctcggcacgg
     1861 ccttgaagcg ctgcggggcc ttgtcggccg ccggctacgc ggcggaggga acggcaatcc
     1921 gcgccgccct gtcgcgcacg ctcgtgacga gagtcgctca gcgcgtggta accggcccga
     1981 attcgaaccc gaatttctcg cccaatctgt tcacggtcca gcgcggctac gaccatgccg
     2041 gcgtcatgca gggcccctat cgcctggcca aatgcatgac gagcgtgaac ctcatgggcg
     2101 tggcaaaggt cgtggcgacc gcgcccggaa cccccgccga acgcgaagcc atgcaggcgg
     2161 tggcccccac cctcgcctcc tgcgtcgacc gcaaccagca ggtcgccctc tccacccgca
     2221 cgctgcgcat ggcgatcgac atcgtctatg cgcagcgaac cttcggcttg ctccggacga
     2281 tgccgcttcc cggccgccgg gtcgtccctc cgccccctgt cggcagcgaa aagtagcgca
     2341 gcccttgaac ccacccgcgc ggcccccatt tgccgcgcca ggaaggatcc tgatcggcga
     2401 gtctcaagcc aaaggaagag cccgcgtgat cgacatcagc cacatattgg tgctgagcca
     2461 acccgagctg ctccgcagcc gggtccgctt cgcgctgttc gaccgcttcg gccggcgcgg
     2521 gggcgaccgc gcgccgcgct gagcccacgc cgcgaccgcc agtcctggct cggcgcgccg
     2581 ccagatgcgg tagcgctagc ggattacctc agacccgttc gggctgagct tgtcgaagcc
     2641 ctgctttgcc ccttcctcgg cggagaagga gggcagcccc cttcggctgg ctgcctgccg
     2701 cagccgctca ggacaggctt cgacaagctc agggcgagcg gtgcgaggtc accgcgccgg
     2761 caaagccttc tcgccgaacg catcggcgag cgcgaagatc gagcggacat ggcgccgggc
     2821 cacttcgagc acgtcggcgc cgtagccgcc gccaagcgtg ctggcgagcg gcaccccccg
     2881 ctcgcgcgcc agccgcgcca ccagcgcgtc gcgcgcgtcg agcccgcctt gggtgagcgc
     2941 gagccggccg agccggtcgc cgctgaacgg atcgaccccg gcctggtaga ggatcaaggc
     3001 gggccggaac cgctcgacga gcggcgtcag cgtcgcctcc agccgatcca gatattcgtc
     3061 gtcccccacc ccgtcggcca gcgccacgtc caccgtggag cgcgccttgc gcaccgggaa
     3121 attcttgtcg gcatggatcg aataagtggc gatatcggcg cggccggcgg tcagcgccgc
     3181 ggtgccgtcg ccctggtgga cgtcgcaatc gacgatcagc acccgctcca ccctcccctc
     3241 ctcgaccagc cgcacggcgg cgacggcgag gtcgttgaac acgcaatagc cggcgccgct
     3301 atcgtggagc gcatggtggc tcccgcccgc gatgttggcg gcatggccgc gcccaatcgc
     3361 ttcgatcgcc gcgcgccagg tgccgccagg cacgcgcacc gcccggcgcg ccaccgcctc
     3421 ggtgaccgca aagccgatcc gccgttcctt ggccgccggc acgcgcgcct cgatcacttc
     3481 ggcgacatag gagggatcgt gcaccgcctc cagccaggcg cgcggcatcg cctcgggctc
     3541 gatccagtcg atcccgcgcc cgctttccag cagcaggtcg cgcaccagcc cgttcttgtt
     3601 ccacgcatag gtgctgccgg cgcgggctgg cgcgacatag tcgggatgat ggacgatcgc
     3661 gatcaccgcc cctaggtagg cggcccgaag gccgggcgcc atcacggccg cggcggagaa
     3721 tgacgtgagc gaccctgtcg tgcgccccga cgtgcgcact ttcctcgatt atctgaacgc
     3781 gctgccgggg ccgcgcagcc accaggtcgg cccgatcgag gcgcgggcga tgatgcacgc
     3841 ctcgcgccat atcgccgacg ccccggtcgg cgagctggcc gtgatccgcg acctcgcggc
     3901 gccgggcccg gcgggcgaga tactgctccg gctctacgat ccgcgggagg agcgggatcc
     3961 cggccccgtg atgctgttcc tccatggcgg cggcttcgtg ctcggcgacc tcgacaccca
     4021 cgcccccttc tgcgccgaaa tggcgcggct gatggacctg ccgctggtgg cggtcgacta
     4081 tcggctggcg cccgaacatc cctggccggc ggcgcccgag gatgcgatcg cggcggcgcg
     4141 ctgggtggcg gagagcccgg cggcgctcgg ccgcgccgtc accggcctgg tcgcgatcgg
     4201 cgacagcgcc ggcggcaatc tcgccatcgt cacctcgctc gcgctgcgcg acgagccggc
     4261 ggcggtgccg gtgctcgcgc aatggccgat ctatccggcc gccgatccgg ccaagggcta
     4321 tccgagcttc accgatttcg gcgaaggcta tctcctgacc cgcgagggca tgcactggtt
     4381 cgaccgatgc tacgccgccg accccaatga ctggcgctat gcgccgctgg tgaagagcca
     4441 gcgcggcatg ccgccgactt tggtggtcac tgccggcctt gatccgatcc gcgaccaggg
     4501 ccgcgcttat gccgccgcct gcgtcgccgc cggcgtcccc accatcttcc gcgaggccga
     4561 gggcaatatc cacgggttca tcaacctgcg gaaggcgctt ccctcttcgg aagcggacat
     4621 acgcggctgc gcggtggcgc tgcacctgat gctgacggag gcgatcgatg gtcgataagg
     4681 aaaccctgcc ctatcggccg gcggcggcgg tgatgctgct caaccgcgac ggcaaggtct
     4741 tcgtcgccca gcgcttggac agcacgctcg aggcctggca gatgccgcaa ggcggcctcg
     4801 acgaaggcga ggagccgctg gccggcgcgc tccgcgaact cgaggaggag accgggatca
     4861 ggcccgagca tgtcgagctg atcgcggccg cgccggccga gttccattac gacctgcccg
     4921 acgacctcat tggcaagatg tggaagggca aatggcgcgg ccagcgccag agctggttcc
     4981 tgatgcgctt caccggcacc gacgcggacg tcaacatcgc caccgccgac ccggagttcc
     5041 gcgcctggaa atgggccgac ccggagtcgc tgccggcgat gatcgtgccg ttcaagaagc
     5101 gcctctacga ggacgtgctg gccgccttcc gccgatattt gtgagccggc ttcgctctgg
     5161 tgcccctggc ccgagtcgaa cgggcactcc ttgcggaact cgattttgag tcgagcgcgt
     5221 ctaccaattc cgccacaggg gcaaccgcgc cggctctagc cgaccgacaa gcgacccgca
     5281 accggggggg agccggtggg gcagcccgga cttcgccgac cccgtcattc cagcgcaagc
     5341 tggaatctca ctgccttccc cccggagacc ccaagaaggg agattccagc gctggaatga
     5401 cggcggcagc ggcggatcca ttgcccgctc agccctgggc ggccgcgcga acgtaacgtt
     5461 ccccggcgaa agccggggtc cagccccaac cctccactgg accccggcct ccgccgggga
     5521 gcaaccgtca tcgaacgcgg atcaaacctg cccagtcacc gctaaaaaag aacaaagaaa
     5581 gaactttcct acagcccctc ccccgcggca tagactcgtc gatgagcgat tcggcccccg
     5641 acgatcccct ccacttcgac cccgtgccct ccgccagcac gcgccatgac ggctggacgc
     5701 cggcgcggca gcggcgcttc atcgccgtgc ttcgggacag cggcaaggtc gccgcggcgg
     5761 cgagggcggc gggcaagagc cgcaagtcgg catacgactt gctgaagcgc gccggaccgg
     5821 acagcggctt cgcccgcgcc tgggacaccg cgcaggggga gcagaggcgc ctgcgcgccg
     5881 ccgacgccgc cgccgccatc gaacgcgccc tccacggcga ggaggtcggc tattattatg
     5941 gcggcctcga gcgcggcgtg cgccgggtct atgacgaccg gctgctgatc gcggccctgc
     6001 gcgccgccgg ccgcgccccg ggcctggcac ggcggcgcaa atgagccgga agcggctcgg
     6061 ttcaccgcaa tttccgcgct acgttgcgcc acctttgtca ccttcgcggc ggccccgccg
     6121 attgaaccga tccgctcccg caacgttttt ggacggcagt tcaaaggatg agagacatgg
     6181 ccggcggttg gtcgcgcgac ggcgcggttc aggatcagat cgacgacacc gtcaaggatg
     6241 cggtgctggc ggcgcgggcg cggctggcgg ccgcggccgg cgccgagagc gagacccatt
     6301 gcatcgagtg cggcgaggag atccccgaag gccgccgccg cgccctcccc ggcgcccgca
     6361 cctgcgtggc ctgccagtcg gcccgcgacc ggcgcagccc cgccgccggc atcaaccgcc
     6421 gcggcagcaa ggacagccag ctgcgctgac gctagcgaag cgcgccccgc gcccgcatcc
     6481 tcagcgcgta aaggagcaaa gccgtcgccg ccgcccagat tgcgacgtgg agcgcgatcg
     6541 ccgcgccgcc gtccatatgg ccggacggca tcggcgaggc gaactggaag agcgtgttca
     6601 ccgcgaggcc ggcgatcgac aggatgaacg cggtcacggc atggcggctg cgcgccagca
     6661 gcaacagcga gccggcgagc ccgccccaga cgccgaacgc ccaggcagcg tccgcccagg
     6721 ccggcgccgc ctcgagccag gcgcgctgcc cttcggtcat ctgcgccatc cagcccgggt
     6781 tgcgggtctg cgacatggtg tagtcgagcg cgccgaaccc gttccagacc gcggcgagcg
     6841 cgcccacgat ccacagatgc acgggcgtct tcaccgatcc gttcatggct tgcctccccc
     6901 gagtcgcgcg gcccggaggg tgtcactccc gccccccgct ggcaagccga tgggcgcccg
     6961 ctgcggtagc ttcgtcgaaa gaggatcagt ttcgtcgtgc cacctttatc gttcgtcgat
     7021 ggcggctttt cccaattgtt ggcgcaggat tatcggggcc cggcgtcggg tgacggcact
     7081 cgcgcgggtc aaagcactgg aatcgggcgg cttccgattt ctggcacgat ccgtgctgag
     7141 gaggccggca tccacaacgg gcaccgcccg cttcgtcaga ggaacagtcg atgcaaagct
     7201 accagcctta ccttttcaac cgcagcgaca ccttcttcgg ggtgtgcgag ggtctcggcg
     7261 aggatttcgg cttcaacgcc aactatctcc gggtggcgct cgctctggcg atcttctgga
     7321 acccggtgat ggtggcgggc ggctatgcgg gtgcgggcgt gatcgtcgcg ctgtcgcgct
     7381 ggctggtccc caatccgcgc cccgcccctg ccgtcgaagc ggccgagacg cccatgccgg
     7441 ccaacaccac cgaaccagcc gaggcgctgc cgctggcagc gtgagcggag cgcgggcgag
     7501 tcactcgccc gcggccccgc cgtcagagca ggccgagcgc cttgaagctg gtccagccgt
     7561 ggcgagacag gatcagatgg tcgtggacca cgacccggag cgtccgtccc acccccgcga
     7621 tcgcgcgggt gatctggaga tcgctctggc tggggctgag gtcgcccgaa gggtggttgt
     7681 ggaccaggat caaggcggtc gcgccgagct cgagcgcccg cttcaatatc tcgcgcggat
     7741 agaaggcggc ctcgttgacg gtgccgtcgg tgacggtctc gccgagcaag gcattctggc
     7801 cgttgaggta gagcacccgc agccgctcga ccggcgcatg gccctggtcg agcatcaaat
     7861 agtcgagcag cgccgccgaa tccgacagga ttggcgccgc cgtcgcctgg ctgcgcaagg
     7921 cgtgcagcat ggcggcgcgg gcggcctgga ggacgcggac cgtgacgctg tcgccgacca
     7981 cccgctgctg cgccgccggc tccgccgcca gcaccgccgc cagcgagccg aattcggcga
     8041 tcagcgctcc ggcggcggcc tccgcccgcg ccgcgcccag ccccgccagg agcgcggcga
     8101 ggtgggtccg gcagctcgac tcccccagtg cttggcccgc tgctggcccc gatgctgggc
     8161 ccggtgctgg ttccaggtcc cgatgaggat ggtgagcatc atcggatagc tccagaaggc
     8221 ctgcaggaag ccgtagccgt tccttggcat ttcgaggtcc gccagcttgg cgaaatggcc
     8281 gagaaggccg atcaattgaa gcgctgctgt ccataatggc caataccgat cggatctgag
     8341 cgcgatcgcg aggaacgccg ccagcaccgc cgcatccacc agcaggatcc ccgtctccac
     8401 gtccgcgaag cggcgtgacg ccgccgatac cgcgatcagg gtcaggatcg atcccgctaa
     8461 taatgtcagt gctcccagct tttcccactt gccgccgcgc cagagcgcgt aggcgacgat
     8521 cgcgaagagg cagaaaaagt agaacagcac tcgcatccgc gtcctacggc cacgcaagcg
     8581 cccggccgtc aacaaggatg cgcgcgcggc cccggttaag cggcgtccct ggaggcgacg
     8641 atctggagcc cgttcgcggc ccaatcctcc gggcattcga ccacgtcgcc gacggcgagt
     8701 tcgcgcaaat cgaggcgggc gagctcggca tgcatgtcga gcagcgactc gcgcgccttc
     8761 gccccgtgcg agatcgcctg gagcgcgcgg tcgagcgctt cgcgtccggt ccgggccggc
     8821 acgcccgagg tccggcgggc gtcgagcagg ctcgccacca gccgcgcggt gtcggccaag
     8881 gcgaggtcat attgatcctc ggagttgcgc acgtcggccg cgatcttgcg ggccttcgcg
     8941 ataaggtgat gacgcaaggc tgttcctttc cgcgcggcgg gggccgcgca tgcgattgag
     9001 acgatgatgt gagacggctt agctgaaggt gcgggcggcc tgtccgacca tccggaccac
     9061 accccaggag gtggcgatca gggcggcgag cgccagcacg acgccgatcg cgatcccgcc
     9121 gatccagagc aggcgggttg cggcaggcag ctcattgcgt tccccttggt ggcgagggaa
     9181 tgggagcatc cgcgctgccg ggtaggggaa agcggccgca aagaccgcct gctcctcccg
     9241 gac
//
LOCUS       DASVRK010000095         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_327869, whole genome shotgun sequence.
ACCESSION   DASVRK010000095 DASVRK010000000
VERSION     DASVRK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327869"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VF589_03125"
     CDS             <1..479
                     /locus_tag="VF589_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256598.1"
                     /translation="LTAGYDRRAAEWSGVVGGLDEGGDADLAWALLALASPDPSVDVG
                     TGRIGAYAGRDDSDGKLRSKMLAAGLAGLGRLDPDSAADVGVNTAAANAWTRQLDRAV
                     QDRQPGSVALLAAIGMQTGGWAGVPPEHLYHIVSALRRVGLEYEARMIAAEAVARL"
     gene            476..1405
                     /locus_tag="VF589_03130"
     CDS             476..1405
                     /locus_tag="VF589_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8256599.1"
                     /translation="MSAAAASAPPASEDRQLIAAFLEMMAAEAGAAHNTLLAYERDLR
                     GASELLGRGLASADAERLEQLGQAWLPLKRATVARKAAALRRFFAFLADEGLREDDPS
                     AALPRPGGERPLPKILDHVAVDALFAEIDRRKAETGGGSASLRLCALVELLYGSGLRA
                     TELVSLPRNAVAPDRPFLILKGKGGRERLVPISDRARAAVREWSALVPRDALWLFPSG
                     KKHLSRVRLYQLLQELGAAAGIPPDRISPHVLRHAFATHLLEGGADLRALQMLLGHAD
                     IATTQIYTHVDSRRLIELVNSRHPLVDVPTRSP"
     gene            1418..2359
                     /locus_tag="VF589_03135"
     CDS             1418..2359
                     /locus_tag="VF589_03135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX8256600.1"
                     /translation="MPIFLDFEKPIAELDARVRELRETAEAGELNIGAEIDRLQAKSD
                     RMLRETYARLTPWQKTQVARHPERPHFKDYVAALVDEFVPLSGDRAFGEDQAIVGGLG
                     KMAGRKVMLIGHEKGDDTASRLKHNFGMAKPEGYRKAVRLMQIADRFGLPVVTLVDTP
                     GAFPGVQAEERGQAEAIARSTEQCLQLSVPLVAAVVGEGGSGGAIALAAANRVLMFEH
                     SVYAVISPEGCASILWRTADKAAEAAEAMRVTAGDLRQLGVIDRVVPEPLGGAHRRPE
                     DAIAALGRAVAEELDALSAMSGEQLRAERRAKFLAIG"
     gene            complement(2443..2625)
                     /locus_tag="VF589_03140"
     CDS             complement(2443..2625)
                     /locus_tag="VF589_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8256601.1"
                     /translation="MKFFKKLIKNEKGATAIEYGLIAALIAVAAITAMSGLGNQLSLT
                     FGGVSSKMGEGMTQID"
     gene            complement(2739..2930)
                     /locus_tag="VF589_03145"
     CDS             complement(2739..2930)
                     /locus_tag="VF589_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8256602.1"
                     /translation="MKRRISSLLQDQRGATAIEYGLIISLVVIAMMASLMQVANVTVG
                     MWNGVSEKVVESNRPAGGT"
     gene            3022..>3403
                     /locus_tag="VF589_03150"
     CDS             3022..>3403
                     /locus_tag="VF589_03150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX8256603.1"
                     /translation="MEKNPTLTVVAAALIDPAGRILLQQRPPGGAMAGLWEFPGGKVE
                     PGELPEAALVRELREELGLAVDPASLAPATFASAELGERHLLLLLYICRQWQGEPEPL
                     AAAALQWLRPEEMHALPMPPADRPL"
BASE COUNT          495 a         1195 c         1162 g          551 t
ORIGIN      
        1 tgctcaccgc cggctacgat cgccgcgcgg ccgagtggag cggcgtggtc ggcgggctcg
       61 acgaaggcgg cgacgccgat ctggcctggg cgctgctcgc gctcgcctcg ccggatccgt
      121 cggtcgacgt cggtaccggc cggatcggcg cctatgccgg ccgcgacgac agcgacggca
      181 agctgcgctc gaagatgctg gcggcgggcc tcgccggtct cgggcggctc gatccggaca
      241 gcgccgccga cgtcggcgtg aacaccgccg ccgccaacgc ctggacccgg cagctcgacc
      301 gcgccgtgca ggaccgccag cccgggtcgg tcgcgttgct ggccgcgatc ggcatgcaga
      361 cgggcggctg ggccggcgtg ccgcccgagc atctctatca tatcgtcagc gcgctgcgcc
      421 gcgtcgggct cgaatatgaa gcgcgcatga tcgcggcgga ggcggtggcg cggctgtgag
      481 cgcggcggct gcgagcgcgc cgccggctag cgaggatcgg cagctcatcg ccgcctttct
      541 cgaaatgatg gcggccgaag ccggcgcggc ccacaacact ttgctcgcct acgagcgcga
      601 cctgcgcggc gcctccgagc tgctcgggcg cggcctcgcc tcagccgatg cggagcggct
      661 cgagcaactg ggccaggcct ggctgccgct gaagcgcgcc accgtcgccc gcaaggcggc
      721 ggcgctgcgc cgtttcttcg ccttcctcgc cgacgagggg ctgcgcgagg acgatccttc
      781 cgccgctttg ccgcggcctg gcggcgagcg tccgctgccc aagatcctcg atcatgtagc
      841 ggtggacgcg ctgttcgccg agatcgaccg ccgcaaggcc gagaccggcg gcgggtccgc
      901 ctcgctgcgg ctctgtgccc tcgtcgagct tctctacggc tccggcctgc gcgcgaccga
      961 actggtgtcc ctgccgcgca acgccgtcgc gcccgaccgg ccgttcctca tcctcaaggg
     1021 caagggcggc cgggagcggc tggtgccgat ctccgaccgc gcccgcgccg ccgtgcgcga
     1081 atggtcggcg ctggtgccgc gcgacgcgct ctggctgttc ccgtccggca agaagcatct
     1141 cagccgcgtc cggctctacc agctgttgca ggagctcggc gccgccgccg gcattccgcc
     1201 cgatcggatc agcccgcacg tcctccgcca cgccttcgcc acccatctgc tcgaaggggg
     1261 cgccgacctg cgcgcgctgc aaatgctgct cggccatgcc gacatcgcca ccacccagat
     1321 ctacacccat gtcgacagcc gccggttgat cgagctggtc aacagccgcc atccgctcgt
     1381 tgacgtaccg acccgctcgc cgtaaccgcc gcgctccatg ccgatcttcc tcgatttcga
     1441 aaagcctatc gccgagctcg acgcccgcgt ccgagagctg cgcgagaccg cggaagccgg
     1501 cgagctcaac atcggcgccg agatcgaccg gctgcaggcc aaatcggacc gcatgctgcg
     1561 cgagacttat gccaggctga cgccgtggca gaagacccag gtcgcgcggc accccgagcg
     1621 accgcacttc aaggattatg tcgccgctct ggtcgacgag ttcgtgcctt tgtccggcga
     1681 ccgcgcgttc ggcgaggacc aggcgatcgt cggcgggctg ggcaagatgg ccggccggaa
     1741 ggtgatgctg atcggccacg aaaaaggcga cgacaccgcc tcgcgcctca agcacaattt
     1801 cgggatggcc aagcccgaag gctatcgcaa ggcggtgcgg ctgatgcaga tcgccgaccg
     1861 cttcgggctg ccggtggtga ctttggtcga tacgccggga gcctttccgg gcgtgcaggc
     1921 ggaggagcgc ggccaggccg aggcgatcgc ccgctcgacc gagcaatgcc tgcaattgtc
     1981 ggtgccgctg gtggcggcgg tcgtggggga gggcggctcc ggcggcgcga tcgcgcttgc
     2041 cgccgccaac cgcgtgctga tgttcgaaca ttcggtctac gccgtcatct cgcccgaagg
     2101 ctgcgcctcg atcctgtggc gcacggccga caaggcggcc gaggcggccg aggcgatgcg
     2161 ggtgacggcc ggcgacctcc gccagctggg cgtgatcgac cgcgtggttc cggagccgct
     2221 cggcggcgcc catcgccggc ccgaagacgc gatcgccgcg ctcggccggg ccgtggccga
     2281 ggagctggac gcgctgtcgg cgatgtccgg cgaacagctg cgcgccgagc gccgggccaa
     2341 gttcctggcg atcggctgag tccccggcgt tctgccccag acaaaagaaa ggggcggcgg
     2401 aaccaggttc cgccgcccct cctttgatcc agcgactaga tgttagtcga tctgggtcat
     2461 gccttcgccc atcttgctcg acacgccgcc gaaggtcagc gacagctggt tgcccaggcc
     2521 cgacatggcg gtgatggcgg caacggcgat gagggcggca atgaggccgt actcgatggc
     2581 ggtggcgccc ttttcgttct tgatcagctt cttgaagaat ttcatgttcc ggtctcctgt
     2641 tccaaaccca ctggaagtcc acacttccat gccgcctttg tattccagtt acgaaggagg
     2701 cattcttcct ttgctagtgc cgagaggttg cgaaatactt aagttccgcc cgccggcctg
     2761 ttgctttcta cgactttttc ggaaacgccg ttccacatgc cgacggtgac gttggctacc
     2821 tgcatcaggc tcgccatcat cgcgatgacg acgagcgaga tgatgaggcc gtactcgatg
     2881 gcggtggcac cccgttggtc ctgcagcagt gacgatatcc ggcgtttcat ttccaggacc
     2941 tccgggcgag cgagcctgtt gcccgctcag ccttggagat aaggcaacgc cgttaatgga
     3001 ttcctatggc acgaggttaa ggtggaaaaa aatccgacgc tcaccgtcgt cgcggccgct
     3061 ttgatcgatc ccgccggccg tattctgctc cagcagcgcc ctcccggcgg agcgatggcg
     3121 gggctgtggg agttccccgg cggcaaggtc gagccgggcg aattgcccga ggccgccttg
     3181 gtgcgcgagc ttcgcgaaga gctcggcctt gccgtcgatc cggcctcgct cgcaccggcc
     3241 actttcgcga gcgccgagct gggcgagcgc cacctgctgc tgctgctcta tatctgccgg
     3301 caatggcagg gcgagcccga gccgctcgcc gccgcggcgc tgcaatggct ccggccggag
     3361 gagatgcacg cgctgccgat gccgcccgcc gaccgcccgc tga
//
LOCUS       DASVRK010000096         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_514708, whole genome shotgun sequence.
ACCESSION   DASVRK010000096 DASVRK010000000
VERSION     DASVRK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514708"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VF589_03155"
     CDS             <1..372
                     /locus_tag="VF589_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX8256604.1"
                     /translation="DPIEKVFALLGCYRRLIVDSGCDYGCPIGSLALELHEPDPAVRR
                     RLADNFAGWTAAVRGCLDEARDRFPADADMGAVAEFVLTVMEGAVMQARTYRDVGHFD
                     RAVEQLRSYFRHMAPAESAGA"
     gene            369..965
                     /locus_tag="VF589_03160"
     CDS             369..965
                     /locus_tag="VF589_03160"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8256605.1"
                     /translation="MKRAAIAALLALAATPAAAAPAPTDVSASRKVEADGSHTLVHEV
                     VVEAEPASVWAAIATAEGWKSWAVPVAWAPQPDIIETSYDPAAKPGDKSTIRQRVLAR
                     IPGRLLLFSTVKAPAAFPDFDTYAKVTSFIELEPAGAGRTRVRLTGVGYADSPAGRKL
                     LGFFEQGNRVSLDQLRTRFRDGPIDWPKRLREERAKGE"
     gene            969..1367
                     /locus_tag="VF589_03165"
     CDS             969..1367
                     /locus_tag="VF589_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX8256606.1"
                     /translation="MPDVNMLAVLLCGVSSMMLGAIWYSPVLFAGAWQRGAGLGDEAL
                     AGGNMALIYGLAFALSLAAAYVFAMFLGTEMGLWPSVAAGGAAGLFWVAAAFGINYLF
                     ERKSLRLWLINGGYHAVQFALFGLILGLFS"
     gene            1446..2087
                     /gene="msrA"
                     /locus_tag="VF589_03170"
     CDS             1446..2087
                     /gene="msrA"
                     /locus_tag="VF589_03170"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8256607.1"
                     /translation="MTRPLSLAALLLLAACGSDYQPELAPSEAQAPAAAAAGTTATAI
                     FAGGCFWCTEADFEKVPGVTGAVSGYTGGRSADPTYEQVSAGGTGHYEAVRVSYDPSR
                     VTYQQLADYYFRTVDPTDGEGQFCDKGESYRTAIFYADNDQRKLADREKAEASRTLGQ
                     AVATAILPAKAFYEAEGYHQDYYKTNSVKYRFYRSRCGRDERLAELWGAKAKG"
     gene            complement(2084..2278)
                     /locus_tag="VF589_03175"
     CDS             complement(2084..2278)
                     /locus_tag="VF589_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256608.1"
                     /translation="MSHIPNSAMPRAVSTAEAGAERASMHEKAGKFARGNRTAIAAVA
                     IGALAAAAIPFARRRAARAD"
     gene            2557..2850
                     /locus_tag="VF589_03180"
     CDS             2557..2850
                     /locus_tag="VF589_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256609.1"
                     /translation="MRKMMIALIAASSAFATAAPAFEGDTDTEQTFSNRGQCQRAMKQ
                     ARNEERRTARTEQGTNSGEFNRTSKGTSRCEAFTDENGQRMFRLVSEDGGGSA"
BASE COUNT          486 a         1035 c         1088 g          492 t
ORIGIN      
        1 gacccaatcg agaaggtttt cgctttgctc ggctgctatc gccggctcat cgtggactcg
       61 ggctgcgatt atggttgccc gatcggcagc ctcgcgctgg aactgcacga gccggacccc
      121 gcggtgcgcc ggcggctcgc cgacaatttc gccggctgga cggcggcggt gcgggggtgc
      181 ctcgacgagg cgcgggaccg gttcccggcc gatgccgaca tgggcgcggt cgccgagttc
      241 gtgctgaccg tcatggaggg cgcggtgatg caggcgcgga cctatcgcga cgtcggccat
      301 ttcgaccgcg cggtcgagca gctgcgaagc tatttccggc acatggctcc cgccgaaagc
      361 gcaggggcat gaaacgcgcg gcgatcgcgg cgctgctcgc cctcgccgcc acgcccgccg
      421 cggccgcgcc cgcgcccacc gacgtttcgg ccagccgcaa ggtcgaggcc gatggttcgc
      481 acacgctcgt ccacgaggtg gtcgtcgagg ccgaaccggc gtcggtgtgg gcggccatcg
      541 ccacggcgga aggctggaag agctgggcgg tgccggtggc atgggcgccg cagccggaca
      601 tcatcgagac cagctacgat cccgccgcca agccgggcga caagagcacg atccgccagc
      661 gggtgctggc gcgcataccg ggacggctgc tcctgttcag cacggtcaag gctcccgccg
      721 cctttccgga tttcgacact tacgcgaagg tcaccagctt catcgagctc gagccggcgg
      781 gggcggggcg aacccgggtg cgcctcaccg gcgtcggcta tgccgacagc ccggccgggc
      841 gcaagctgct gggcttcttc gagcagggta accgcgtctc gctcgatcag ttgcggacgc
      901 ggttccggga cggaccgatc gactggccga agcggctccg cgaggagcgc gcgaaagggg
      961 agtaggacat gccggacgtg aacatgctcg cagtgctgct gtgcggcgtc agctcaatga
     1021 tgttaggcgc gatctggtat tcgcccgtgc ttttcgccgg agcttggcag cgcggcgccg
     1081 gccttggcga cgaggcgctg gcggggggca atatggcgct gatctacggc ctcgccttcg
     1141 cgctgagcct cgccgccgcc tacgttttcg caatgttcct cgggaccgaa atggggctgt
     1201 ggccatcggt ggcggcgggc ggcgcggcgg ggctgttctg ggtcgcggcg gcgttcggga
     1261 taaactatct gttcgagcgc aaaagcctcc ggctgtggct gatcaacggc ggctatcatg
     1321 ccgttcaatt cgccttgttc gggctgatcc tcggcctctt ttcctgaggc cgcgcccgac
     1381 caggcgggag gcgagccgaa gtcctcatcc ttgatttcgc gggccgccgc gccagatgca
     1441 gcggcatgac ccgccccctc agccttgccg ctttgctcct gctcgctgcc tgcgggtccg
     1501 attatcagcc cgagctcgcc ccctcagaag cgcaagcgcc ggccgcggcc gcggcgggga
     1561 ccaccgccac cgcgatcttc gccggcggct gcttctggtg caccgaggcc gatttcgaga
     1621 aggtgccggg cgtgaccggg gctgtgtccg gctataccgg cggccgctcg gccgacccga
     1681 cttacgagca ggtttcggcc ggcggcaccg gccattatga ggcggtgcgg gtgagctacg
     1741 acccgtcgcg cgttacctat cagcagctcg ccgactatta tttccgcacc gtcgacccga
     1801 ccgacggcga aggccagttc tgcgacaagg gcgaaagcta ccggaccgcg atcttttacg
     1861 ccgacaatga ccagcgcaag ctcgctgatc gcgaaaaggc ggaggcgagc cgcaccctgg
     1921 gccaggcggt ggcgaccgcc atcctccccg ccaaggcctt ttacgaggcc gagggctacc
     1981 accaggatta ttacaagacg aacagcgtca aataccgctt ctaccgcagc cgctgcggcc
     2041 gcgacgaacg gctggccgaa ctgtgggggg cgaaggcgaa gggctagtcc gcccgcgccg
     2101 cccgcctgcg ggcgaatgga atcgcggccg ctgcgagggc accgatggct acggcggcga
     2161 tcgcggtgcg gttgccgcgc gcgaacttgc ctgctttttc gtgcatcgaa gcgcgttcgg
     2221 cgccggcttc ggcggtcgaa accgcgcgcg gcatggcgga attggggatg tgggacattt
     2281 cgggtctctc cagggcttta gctgcgcaac gacaatggcg gcggccggtt cctgcctgga
     2341 gccggcggga gaggaccgat gatcggagcg atagcaggcg ataaggtctc acggcgatgc
     2401 tcgcccgttc ggaagccgcc cgcaccgata ggggcacggc accattcaag accaaaagtt
     2461 ctttaaacgc ggcgacttac gcccatcggc gaacgatcgc gaggctgctt tgttgactcc
     2521 gccgccgcag gtaagcggca agccaggaga tgatttatgc gcaagatgat gattgcattg
     2581 atcgcggcca gctccgcgtt cgccacggcg gccccggcct tcgagggcga caccgatacc
     2641 gagcagacgt tcagcaaccg cggccagtgc cagcgcgcga tgaaacaggc ccgcaacgag
     2701 gagcgccgca cggccaggac cgagcagggc accaattcgg gcgagttcaa ccgcacgtcc
     2761 aagggcacgt cgcggtgcga agccttcacc gacgagaacg gccagcgcat gttccggctc
     2821 gtcagcgagg atggcggcgg aagcgcctaa gcacgttcgt tgcggggccc tgcgcgatgc
     2881 gccgggcccc gctccggtct aaagcaaccc tgctccgcct ccgggcgggg ctattttttt
     2941 gccagcgcgc cgagcgccgc cgtcaccatg gcctcggtgg cggtcgagat ggtcggctcc
     3001 gggtccggcg cccagaacgg gctgtgcagc gacggcagct tggccgggtc gccgccggcc
     3061 ttctgccatt gcgcctcggg cacgccgccg acccagaaga t
//
LOCUS       DASVRK010000097        12927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_389769, whole genome shotgun sequence.
ACCESSION   DASVRK010000097 DASVRK010000000
VERSION     DASVRK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389769"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..475
                     /locus_tag="VF589_03185"
     CDS             41..475
                     /locus_tag="VF589_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX8256610.1"
                     /translation="MFKKITLAAAAAVGALTAMPAAAEAQYYRGGGYYNPHQGGGYAY
                     GQGYRRYQPGDHYQGQRRHYGQRYYGQRHYGQRHYQGNRGYYGQGYGRYCRDSGSGGT
                     ILGAIAGGLLGSEIAGRGDRTEGAIIGGAAGALAGRAIDRNC"
     gene            complement(548..2377)
                     /gene="cobT"
                     /locus_tag="VF589_03190"
     CDS             complement(548..2377)
                     /gene="cobT"
                     /locus_tag="VF589_03190"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX8256611.1"
                     /translation="MADRNNPLETFRQVLTGASRAIAREPELELGFTAEAPSSSGKNV
                     KVPMPGRTLPEREVAEARGYADAAALKLRHHDAALHARNAPADEAARAVFGAAEQARV
                     EALGARAMAGVRANLNRATEMRMRTDPITRARSRAEVPLATAIALMVRERLTGEAPPE
                     AAREGLKLISDWIEDKAGADLDALGLALDDQAAFATLATKVLKDLELVEGEYEADRDP
                     DEGEEGEGEDQDEADSADDENEEEGGGDGEAEMRVEQGDSSEEQGDSDYSDAEMSEGD
                     EGAGEEGEEGMLPIRPNRPLSDLPPQFDYKMFTTRFDEVIEAGELCDPEELGRLRAYL
                     DQQLIHLQGAVTKLANRLQRRLMAQQSRSWDFDQEEGMLDAARLARVIVNPAHSLSYK
                     VEKDTDFRDTVVSLLIDNSGSMRGRPISIAAISADILARTLERCGVKTEILGFTTRAW
                     KGGQSRERWLAEGRPPHPGRLNDLRHIVYKKADEPWRRARKNLGLMMREGLLKENIDG
                     EALLWAHNRLIARPEERRILMVISDGAPVDDSTLSVNSGTYLERHLRQVIGWIEARSP
                     VELAAIGIGHDVTRYYSRAVTIMDAEQLGGTMVEQLAALFDAG"
     gene            complement(2455..3459)
                     /gene="cobS"
                     /locus_tag="VF589_03195"
     CDS             complement(2455..3459)
                     /gene="cobS"
                     /locus_tag="VF589_03195"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEX8256612.1"
                     /translation="MADLANANPESRSRPLLDAPDKIIKVRDLFGVDSDMEVPAFSEA
                     DERVPDLDPAYVFDPDTTLAICAGFAKNRRVMVQGYHGTGKSTHIEQVAARLKWPTIR
                     INLDAHISRIDLVGRDAIVLREGQQVTEFREGLLPWALQTPTALVFDEYDAGRPDVMF
                     VIQRVLETEGKLTLLDQNRVIRPNPWFRLFATTNTVGLGDTTGLYHGTQQINQGQMDR
                     WNIVVTLNYLPAATEALIVLAKSGEYDHEGGRQEVERMIKVADLTRQGFISGDISTVM
                     SPRTVISWAQNALIFNDVGFAFRLTFLNKCDEAERPLIAEYYQRVFGKDLPESVVGKA
                     "
     gene            complement(3479..4072)
                     /locus_tag="VF589_03200"
     CDS             complement(3479..4072)
                     /locus_tag="VF589_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8256613.1"
                     /translation="MASSGKRQTRFHGRVEGQGHCSHPGCAGSGEFRAPPAGGRRSGF
                     DGPGDWRWLCLDHVREFNNGYNYFSGMSSEEIEAAQTPYGGWDRETRAFAAAGSPPPR
                     WADFTDPLDAIGARFRRPAAEAARRDGRELSEADRKSLRVLGLGTDTDRRALRQRYAD
                     LVRRYHPDRNGGDRSHEKALQAVIEAYTQLKSRPAFA"
     gene            4111..4395
                     /locus_tag="VF589_03205"
     CDS             4111..4395
                     /locus_tag="VF589_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX8256614.1"
                     /translation="MRDPLSGPVAAEITRRLTESLAPAHLEVRNNSEKHRGHAGYDGS
                     GESHFTVEIVAARFAGMSRLERQRAVNAALGDLLEHRVHALAIKAKAPGE"
     gene            4392..5294
                     /locus_tag="VF589_03210"
     CDS             4392..5294
                     /locus_tag="VF589_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256615.1"
                     /translation="MTSELQTTRIGLPTGVTLKVRFGGPEDAEPLIFLHGFPESHRTW
                     RHQLANLAADYRVVAPDQRGFGGSDKPDGVEAYKTDRIVEDLIALADALGIGGFTLVG
                     HDWGGAAAWLAALRHPDRVKRLVIVNAPHPLIFQKSMFDDEAQRAASQYIRAFRNPGI
                     EAAIKAMGLDAFLEKSFGGHVDLRLISEAERQAYLDDWSQPGALTAMLNWYRASNIDV
                     PEMDEKGHKPLWTHAPFPKLKMPVLVVWGMKDKALLPIQLDGLDDLVEDLRIVRELQA
                     GHFIPWEKPEAVTSAVREFLAETP"
     gene            5354..5809
                     /gene="nusB"
                     /locus_tag="VF589_03215"
     CDS             5354..5809
                     /gene="nusB"
                     /locus_tag="VF589_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX8256616.1"
                     /translation="MSQASARSRSRSAARLAAVQALYQQEMEKTPLPTLLHEFHHHRL
                     GATIEDVEYEDAEVDFFDDLVKGVDARREELDGLIAARLAKDWSLARLDKPMRQILRA
                     GAYELVARIDVPTGSVISEYVDVAKAFYDKREAAFVNGLLDAVAKDARR"
     gene            5843..6799
                     /gene="thiL"
                     /locus_tag="VF589_03220"
     CDS             5843..6799
                     /gene="thiL"
                     /locus_tag="VF589_03220"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX8256617.1"
                     /translation="MTSESAFIDALRAIAAAPGARGLLDDAAVLEVGGVTLVLTHDML
                     VEGVHFLPGDPPGDVAWKLVAVNLSDLAAKGARPVAALLGYSLGEEEWDRAFADGLGL
                     ALDAFGMALLGGDTVSVPAGSARAFGLTAIGEAVGPVPSRAGAAAGDGLWVTGAIGDA
                     GAGLRIAKGELAGPAALVERYRQPRPRLEAGQALAPLVAAMMDLSDGLLIDAERIAAA
                     SGVAAEIDIDTVPLSAERIALLGDDRAARLDAATGGDDYELLFAAPPVRSTEILALGE
                     ALGLPFSRIGRLVEGAGLRLLDRGEPVPLPGRLGWEHVAGLR"
     gene            7000..9177
                     /locus_tag="VF589_03225"
     CDS             7000..9177
                     /locus_tag="VF589_03225"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX8256618.1"
                     /translation="MTVVLIAIACGLAAVLYGVVTSRQVLASSAGNEKMQDIAAAIQE
                     GAKAYLGRQYTTIAVVGVVVAVLVLLFLGLTEAIAFAIGAILSGAAGYIGMNISVRAN
                     VRTAEAARTSLQRGLTVAFRAGAITGMLVAGLALLAIAGFFYFLVEIGGADLGEAADR
                     REVVNALVALAFGASLISIFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIA
                     DNVGDNVGDCAGMAADLFETYVVTVGATMVLIALLVAGTAAELTSLMALPLIIGGVCI
                     LTSIVGTYFVRLGRKQSIMGALYKGFWVTAALSVPAIYYVMTQTLGDMNAVIGGAREA
                     AGFEVEGQVARTAVTDGFTGMDLFWCSLVGLAVTGLLVWITEYYTGTNYRPVRSIAKA
                     SETGHGTNVIQGLAISLESTAMPTIVICAGIIVSYQFAGLIGIAFAATAMLALAGMVV
                     ALDAYGPVTDNAGGIAEMAHMEDEVRTRTDALDAVGNTTKAVTKGYAIGSAALAALVL
                     FGAYTTDLQEFSTELGIGPAGIDFSLSNPYVVVGLLLGALLPYLFGAMGMTAVGRAAG
                     DVVIDVRNQFRENPGIMNGTSRPNYARTVDLVTRAAIKEMIVPSLLPVLAPILVYFVI
                     AAIAGREQGFAALGALLLGVIVSGLFVAISMTSGGGAWDNAKKYIEDGNYGGKGSEAH
                     KAAVTGDTVGDPYKDTAGPAVNPMIKITNIVALLLLAALAGGG"
     gene            9486..11876
                     /locus_tag="VF589_03230"
     CDS             9486..11876
                     /locus_tag="VF589_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256619.1"
                     /translation="MTLISELARARTEEDVKDAYIKALGLKSYFKGLVDIQTEEVWFE
                     AKDAPTPPIVMFGQLLVYVRAARKRGEAIPAFLAVIDREKAAIMPTEKALPLLDDKSI
                     VWPKSGSGAGRELAAQIAPYVQTHIVEYEIAHDEAAFVKAVKDAISERRIIRTPITPD
                     NLRQVFDRWLDMVGSELGAVNPADYAVLFFADIMHDGKNEAMNNLPARLLFSGEKPVF
                     LLDGQTYELASERGYRNFWAIYHRPPDQKHRHYLLERRDSLLPVDEQKFKGAYYTPLH
                     IVDKAYDQLVATLGPNWQDRFIVWDMCAGVGNLEAKHSNLRNVFMSTLDQADVTIMRS
                     NPAFAGATIFQYDYLNDDVTDFGEIDYSLSDKLPRVLRQAIADAGEGKKGAKPILVLI
                     NPPYAEAGSRTDKAIKKSVSSTRIGAWMNGAGYAYAAKELFVQFMTRAAKELPNAWLA
                     MFSTLKYVNSDNFEPFRARWKATYKGGFITHSKAFDGLKGNFPIGFLIWDTGQKTEID
                     AVPVRVLDKDGLEIGSKVFHNVPRDQFLSKFIARKKDFTHEVVPLVNAIVPTQRIETV
                     RNTHWSRGAVAHFLCHGNDLQQAGTLTAILSSTHDIGFTGAYFVTEQNLWQAAVVFAV
                     RRLIKPTWLNDRDQFLQPSQPLTDQFKSDCLVWMLFNGSNLSAGADGLHWNGRDWSLV
                     NHFIPYTEAEVGATGRFESDFMVRYMADMNFSPEAQAVLDEGRKLWTRFHETQFPRRI
                     REDYKLGLNAGWYQIRRALEAYGDAEWTNFDPFKTAYANLSAKLRPMVYELGFLPR"
     gene            12274..12492
                     /gene="infA"
                     /locus_tag="VF589_03235"
     CDS             12274..12492
                     /gene="infA"
                     /locus_tag="VF589_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX8256620.1"
                     /translation="MAKEELLEMRGQVVELLPNAMFRVKLENDHEILGHTAGKMRKNR
                     IRVLVGDEVLVELTPYDLTKGRITYRFK"
     gene            12496..>12927
                     /locus_tag="VF589_03240"
     CDS             12496..>12927
                     /locus_tag="VF589_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX8256621.1"
                     /translation="MAARAPRLVLASASPRRLELLARIGIVPDEVAPAAIDETPRRGE
                     LPANYAARMAAEKAAAVERPGSLVLAADTVVAAGRRILPKAEDEATARAALALLSGRR
                     HRVLSAVTLIDGEGKARHRLSTTIVTFKRLADEDLDAYLASG"
BASE COUNT         2109 a         4379 c         4168 g         2271 t
ORIGIN      
        1 tgccggttcg ccggcccgat aggacaagag gagatattcc atgttcaaga agatcacgct
       61 cgccgcagcc gccgcagtcg gtgcattgac cgccatgccg gctgccgccg aggcgcaata
      121 ttatcgcggt ggcggctatt acaatccgca tcagggcggt ggctatgcct atggccaggg
      181 ctatcgccgc tatcagccgg gcgaccatta tcagggtcag cgtcgtcatt acggccagcg
      241 ctattacggt cagcgtcatt acggccagcg ccattatcag ggcaaccgcg gctattatgg
      301 ccagggctat ggtcgctact gccgtgacag cggctcgggc ggcaccatcc tcggcgccat
      361 cgccggcggt ctgctcggca gcgagattgc cggccgcggc gaccgcaccg aaggcgccat
      421 catcggcggc gccgcgggcg ctctcgccgg ccgcgccatc gaccgcaact gctaaagctt
      481 tccctcgagt agcgtaacga gggcggggcc cggtgcgagc aatcgcaccg ggccctgtgt
      541 ttatgggcta gccggcgtcg aacaaggcgg cgagctgttc aaccatggtg ccgccgagct
      601 gctcggcatc catgatcgtc accgcccggc tataatagcg ggtgacgtcg tggccgatgc
      661 cgatcgcggc cagctcgacc ggcgagcgcg cttcgatcca gccgatcacc tgccgcaaat
      721 gccgctccag ataggtgccg ctgttgaccg acagcgtcga atcgtcgacc ggcgcgccgt
      781 ccgagatcac catcaggatg cgccgctcct cgggccgggc gatcaggcga ttatgcgccc
      841 acagcaaagc ttcgccgtcg atattctcct tgagcagccc ctcccgcatc atcagcccaa
      901 gattcttgcg ggcgcggcgc cacggctcat cggccttctt gtagacgatg tgacggaggt
      961 cgttgagccg gccaggatgc ggcggccgcc cctcggccag ccagcgctcg cgcgactggc
     1021 cgcccttcca ggcgcgggtg gtgaagccta atatctcggt cttgacgccg cagcgctcga
     1081 gcgtgcgggc gaggatgtcg gccgagatcg ccgcgatcga gatcggccgg ccgcgcatcg
     1141 atcctgaatt gtcgatcagc aggctgacca cggtgtcccg gaagtcggtg tccttctcga
     1201 ccttgtagct gaggctgtgg gccgggttga cgatcacgcg ggcgagccgc gcggcatcga
     1261 gcatgccctc ttcctggtcg aaatcccacg agcgcgattg ctgcgccatc agccgccgct
     1321 ggaggcggtt ggcgagcttg gtgaccgcac cctgcaaatg gatgagctgc tggtcgagat
     1381 aggcgcgcag ccgcccgagc tcttcgggat cgcacagctc gccggcctcg atcacctcgt
     1441 cgaagcgggt ggtgaacatc ttgtagtcga attgcggcgg caggtcggac agcggccggt
     1501 tcgggcggat cgggagcatc ccttcctcgc cctcctcgcc ggcgccctcg tcgccttcgg
     1561 acatctccgc gtccgaatag tcgctgtcgc cctgctcctc gctcgaatcg ccttgttcga
     1621 cgcgcatctc ggcctcgccg tcgccgccgc cttcctcctc attctcgtcg tcggcggagt
     1681 cggcttcgtc ctggtcctcg ccctcgccct cctcgccttc gtcgggatcc cggtccgcct
     1741 catattcgcc ctcgaccagc tcgaggtcct tcagcacctt ggtggcgagc gtggcgaagg
     1801 cggcctggtc atcgagcgcg aggccgagcg cgtcgaggtc ggcgccggct ttgtcctcga
     1861 tccagtcgga gatcagcttc agcccttcgc gcgccgcctc gggcggggcc tcgccggtca
     1921 gccgctcgcg gaccatcaag gcgatcgcgg tcgccagcgg gacttcggcg cggctgcgcg
     1981 cgcgggtgat cggatcggtc cgcatccgca tctcggtggc gcggttgagg ttcgcccgca
     2041 cgcccgccat cgcccgcgcg ccgagcgctt cgacccgggc ctgctcggcg gcgccgaaca
     2101 cggcgcgggc ggcctcgtcg gccggcgcgt tgcgggcgtg gagcgcggcg tcgtggtggc
     2161 ggagcttgag cgcggccgca tcggcatagc cgcgcgcctc cgccacttcg cgctcgggca
     2221 gggttcgccc gggcatcggc accttcacat tcttgcccga gctcgacggc gcctcggcgg
     2281 tgaagccgag ctccagctcc ggctcccgcg cgatcgcccg cgacgcgccg gtcagcacct
     2341 ggcggaaggt ttcgaggggg ttgttgcggt cggccatcag ttccccggcg aaggccgggg
     2401 tccagaccgg ccgctgaact gggccccggc cttcgccggg gaacaaacgc tcatttacgc
     2461 cttcccgacc acgctctcgg gcagatcctt gccgaacacg cgctggtaat attcggcgat
     2521 gagcggccgc tcggcctcgt cgcatttgtt caggaaggtc aggcggaagg cgaagccgac
     2581 atcgttgaag atgagcgcgt tctgcgccca gctgatcacc gtgcgcgggc tcatcaccgt
     2641 cgagatgtcg ccgctgatga aaccctgccg ggtgaggtcg gcgaccttga tcatccgctc
     2701 gacctcctgg cggccgcctt catggtcata ctcgcccgat ttggcgagca cgatcagagc
     2761 ctcggtcgcg gccggcaggt aattgagcgt gaccacgatg ttccagcggt ccatctgacc
     2821 ctggttgatc tgctgggtgc cgtgatagag gccggtcgtg tcgccgaggc cgaccgtgtt
     2881 ggtggtcgcg aacagccgga accagggatt ggggcggatc acccgattct ggtcgagcag
     2941 ggtcaatttg ccctcggtct cgagcacgcg ctggatcacg aacatcacgt ccgggcggcc
     3001 ggcatcatat tcgtcgaaca ccaaagcggt cggcgtctgc agcgcccagg ggagcaggcc
     3061 ctcgcggaat tcggtcacct gctggccctc gcgaagcacg atcgcatcgc ggccgaccag
     3121 gtcgatgcgg ctgatatggg catcgaggtt gatgcggatc gtcggccatt tcagccgcgc
     3181 cgccacctgc tcgatatggg tcgacttgcc ggtgccgtga tagccctgca ccatgacgcg
     3241 gcggttcttg gcgaagccgg cgcagatggc gagcgtggtg tccggatcga acacataagc
     3301 cgggtcgagg tccgggacgc gctcgtcggc ctcgctgaac gccggcactt ccatgtcgct
     3361 gtcgacgccg aacaggtcgc gcaccttgat gatcttgtcg ggcgcgtcga gcagcggccg
     3421 cgagcggctt tcggggttgg cattggcgag gtcggccatt ctcttctctt tcgggcgttc
     3481 aggcgaaggc ggggcgggat ttgagctgcg tataagcttc gatcaccgcc tgcaaagcct
     3541 tctcgtggct cctgtcgccg ccgttacgat cgggatggta gcggcgaacg aggtcggcat
     3601 aacgctgcct cagcgcgcgc cggtcggtgt cggttccaag ccccagcacc cgcagcgact
     3661 tgcggtcggc ttcgctcaat tcgcggccgt cccggcgcgc cgcttcggcg gccgggcggc
     3721 ggaagcgggc gccgatcgca tccagcggat cggtgaagtc ggcccagcgc ggcggcggcg
     3781 atccggccgc ggcgaaggcg cgcgtctccc ggtcccagcc gccatagggg gtctgcgccg
     3841 cctcgatctc ctcgctgctc atgccgctga aataattata gccgttgttg aactcgcgga
     3901 catgatcgag gcacagccag cgccaatcgc ccgggccgtc gaagcccgag cggcggccgc
     3961 ccgccggcgg cgcgcggaac tcgcccgatc cggcgcagcc ggggtgcgag cagtgacctt
     4021 gcccctccac ccggccatga aaccgggtct gccttttgcc cgaactcgcc accaactgcc
     4081 tttcccgtcc aaacgcccta tatagtccgg atgcgcgatc ctttaagcgg gccggtagcg
     4141 gccgaaatca cgagacggct gaccgaatcg ctcgctccgg cgcatctgga agtgcgcaac
     4201 aacagcgaga agcatcgcgg ccatgccggc tatgacggca gcggcgaaag ccatttcacg
     4261 gtcgagatcg tcgccgcccg attcgcgggc atgagccggc tcgagcggca gcgcgccgtc
     4321 aacgccgcct tgggcgacct gctcgaacac cgcgtccacg cgctcgcgat caaggcgaag
     4381 gccccggggg aatgacgagc gagcttcaga ccacccgcat cggccttccg accggcgtca
     4441 ccctgaaggt gcggttcggc gggcctgaag atgccgagcc gctcatcttc ctccacggct
     4501 ttcccgaatc gcaccgcacc tggcgccacc agctcgccaa cctcgccgcc gactatcggg
     4561 tcgttgctcc ggaccagcgc ggcttcggcg gctccgacaa gcccgacggg gtcgaggcct
     4621 acaagaccga ccggatcgtc gaggatctga tcgcgctcgc cgacgcgctc ggcatcggcg
     4681 gcttcacgtt ggttggccac gattggggcg gcgccgccgc ctggctggcg gctttgcgcc
     4741 atcccgaccg ggtgaagcgc ctcgtcatcg tcaacgcgcc gcatcctttg atcttccaga
     4801 agagcatgtt cgacgacgag gcccagcgcg ccgcctctca atatatccgc gccttccgca
     4861 atccgggcat cgaggccgcg atcaaggcga tggggctcga cgccttcctc gagaagagct
     4921 tcggaggcca tgtcgacctg cggctcatct cggaggcgga gcggcaggcc tatctcgacg
     4981 actggtcgca gccgggtgcg ctgacggcga tgctcaactg gtaccgcgcg tcgaacatcg
     5041 acgtgccgga gatggacgag aagggccaca agccgctctg gacccacgcg ccgttcccga
     5101 agctcaagat gcccgtgctc gtcgtctggg gaatgaagga caaggcgctg ctcccgatcc
     5161 agctcgacgg cctcgacgac ctggtcgagg atctgcggat cgtccgcgaa ctgcaggccg
     5221 gccacttcat cccatgggag aagcctgagg cggtgaccag cgcggtccgg gagttcctcg
     5281 ccgaaacgcc ttaactctaa cgaggggaat aaactcttgg gctcgcgcac ccgcccggct
     5341 atggccgccg cccatgtcgc aagcctccgc ccgctcccgc tcccgctccg ccgcccggct
     5401 cgccgccgtg caggcgctct accagcagga gatggagaag acgccgctgc cgactttgct
     5461 ccacgaattc catcatcacc ggctcggcgc gaccatcgag gatgtcgaat atgaggatgc
     5521 cgaggtcgat ttcttcgacg atctggtgaa gggcgtggac gcgcgccgcg aggagctgga
     5581 cggcctgatc gccgcgcggc tcgccaagga ttggtcgctg gcgcggctcg acaagccgat
     5641 gcggcagata ttgcgcgccg gcgcctatga gctggtcgcc cgcatcgacg ttcccaccgg
     5701 cagcgtgatc tccgaatatg tcgacgtcgc caaagccttt tacgacaagc gcgaagcggc
     5761 cttcgtcaac ggcctgctcg acgccgtcgc caaggacgcg cggcggtgaa gccggccctc
     5821 ccccgcgccg gggaggattg aggtgacgtc cgaatccgct ttcatcgatg cgctgcgcgc
     5881 catcgccgcc gcgcccggcg cgcgcgggct gctcgacgac gcggcggtgc tggaggtcgg
     5941 cggcgttacc ctcgtcctca cccacgacat gctggtcgag ggcgtccatt tcctccccgg
     6001 cgatccgccc ggcgacgtcg cctggaagct ggtggcggtg aacctctccg atctcgccgc
     6061 caagggcgca cggccggtcg ccgccttgct aggctacagc ctcggggagg aggaatggga
     6121 ccgcgccttc gccgacggcc tcgggctcgc gctggacgcg ttcggcatgg ccctgctcgg
     6181 cggcgacacg gtgtcggtgc cggccgggtc ggcgcgcgcc ttcggcctca ccgcgatcgg
     6241 cgaggcggtc gggccggtgc cctcgcgcgc cggtgccgcc gcgggcgacg ggctgtgggt
     6301 aacgggcgcg atcggcgatg ccggcgccgg gctccgcatc gcgaaggggg aactcgccgg
     6361 gccggccgcg ctggtcgagc gctaccgcca gccgcggccg cggctggagg cggggcaggc
     6421 actggcgccg ctggtcgcgg ccatgatgga cctgtccgac ggcctgctga tcgacgcgga
     6481 gcggattgcg gccgccagcg gcgtggccgc cgagatcgat atcgacaccg tcccactctc
     6541 ggcggagcgg atcgcattgc tcggtgatga tcgtgcggcg cgtctcgacg ccgccaccgg
     6601 gggcgacgac tacgaattgc tgttcgcggc cccgcctgtc cgttccaccg aaatcctggc
     6661 gctcggcgag gcgctcggcc tgccgttcag ccggatcggc cgcctcgtgg aaggcgcggg
     6721 cctccggctg ctcgaccggg gcgagccggt gccgctgccg ggccggctcg gctgggagca
     6781 tgtcgccggg ctccgctaaa ggattcggag caccgtcccg gattgcagca cctcgccgac
     6841 aagtgtccgg caacggttga aacatcgccg cttcccacct aagacgcgcg ccacatccgg
     6901 gcgcgttcgc ttccaggggc gtttcgccct gcccggcttc ggacttgcgt cgaagcatcg
     6961 cttcgcggag gtccacattt tcaggggaag gacgttttca tgaccgtcgt gctcatcgcc
     7021 attgcctgcg gattggccgc agtgctttac ggcgtcgtca ccagccgcca ggtgctcgcc
     7081 tcctcggccg gcaatgagaa gatgcaggat atcgccgccg ccatccagga gggcgccaag
     7141 gcctatctcg gccggcaata taccaccatc gccgtcgttg gcgtcgtggt cgcggtgctg
     7201 gttctgctct tcctcggcct caccgaagcg atcgccttcg cgatcggcgc gatcctgtcg
     7261 ggggcggcag gctatatcgg catgaacatc tcggtccgcg ccaacgtccg caccgccgag
     7321 gcggcgcgca ccagcctgca gcgcggcctg accgtcgcgt tccgcgcggg cgcgatcacc
     7381 ggcatgctcg tcgccggcct cgccctgctc gccatcgcgg gcttcttcta cttcctcgtc
     7441 gagatcggcg gcgccgacct cggcgaggcc gcggaccgcc gcgaagtcgt caacgcgctg
     7501 gtcgccttgg cgttcggcgc ctcgctcatc tcgatcttcg cccgcctcgg cggcggcatc
     7561 ttcaccaagg ccgccgacgt cggcgccgac ctggtcggca aggtcgaggc cggaatcccg
     7621 gaagacgatc cccgcaaccc ggcggtgatc gcggataacg tgggcgacaa tgtcggcgac
     7681 tgcgccggca tggccgccga cctgttcgaa acctatgtcg tcaccgtcgg cgccaccatg
     7741 gtgctgatcg cgctcttggt ggccggcacg gccgccgagc tcaccagcct catggcgctg
     7801 cctttgatca tcggcggcgt gtgcatcctc acctcgatcg tcggcaccta tttcgtgcgc
     7861 cttggccgca agcaatcgat catgggcgcc ctctacaagg gcttctgggt caccgcggcg
     7921 ctttccgttc cggccatcta ctacgtcatg acgcagactt tgggcgacat gaacgccgtc
     7981 atcggcggcg ctcgcgaagc cgccggcttc gaggtcgagg gccaggttgc ccgcaccgcg
     8041 gtgaccgacg ggttcaccgg catggatctg ttctggtgct cgctcgtcgg cctcgccgtc
     8101 accggcctgc tggtgtggat caccgaatat tataccggca ccaactaccg cccggtccgc
     8161 tcgatcgcca aggcttcgga gacgggccac ggcaccaacg tcatccaggg cctggcgatc
     8221 agcctcgaat cgaccgccat gccgaccatc gtcatttgcg cgggcatcat cgtctcctac
     8281 cagtttgccg gcctgatcgg catcgccttc gcggccaccg cgatgctggc gctggccggc
     8341 atggtggtcg cgctcgacgc ttacggtccg gtcaccgaca atgccggcgg catcgccgag
     8401 atggcgcata tggaggacga ggtccgcacc cgcaccgacg cgctcgacgc ggtcggcaac
     8461 accaccaagg cggtcaccaa gggctatgcg atcggctcgg ccgcgctcgc cgcgctcgtg
     8521 ctgttcggcg cctacaccac cgacttgcag gaattttcga ccgagctcgg catcggcccg
     8581 gccggcatcg atttctcgct gtccaacccc tacgtggtcg tcggcctgct gctcggagcg
     8641 ttgctgccct atctgttcgg cgcgatgggc atgaccgcgg tcggccgcgc cgcgggcgac
     8701 gtggtgatcg acgtgcgcaa ccagttccgc gagaatccgg gcatcatgaa cggcaccagc
     8761 cggccgaact acgcgcgcac cgtcgacctc gtcacccgcg cggcgatcaa ggagatgatc
     8821 gtgccttcgc tgctgccggt gctggcgccg atcctcgtct atttcgtgat cgccgcgatc
     8881 gccggccgcg agcagggctt cgccgctttg ggcgcgctgc tgctcggcgt gatcgtctcc
     8941 ggcctgttcg tcgccatctc gatgacctcg ggcggcggcg cctgggacaa tgccaagaaa
     9001 tatattgagg acggcaacta cggcggcaag ggctcggagg ctcacaaggc cgccgtcacc
     9061 ggcgacacgg tcggcgatcc ctacaaggac acggccggcc cggcggtgaa cccgatgatc
     9121 aagatcacca acatcgtcgc cctgctgctg ctggcggcgc tggcgggtgg gggctgaggc
     9181 tgaaccggag actagcagcg gatcgtatgc gatctgctgc ttaggcgagg aaaggtcgaa
     9241 gcccggccgg cgggcgaccg agctcggccc tcggccgacc ccaccgatgg cgcgaggccg
     9301 ccctcgcgcc ttccttccaa gccccgctac ggcgggggtt ttcttttgct ccctcgactt
     9361 gcagtgttgc gccgtggagg gcgcgtgagc gcctagcatc tcacgcgcac cgtcatctcg
     9421 cgccaggatg accgcgaagc gaggagatgt ctgctcaagg cgagagaaga aagcgaccaa
     9481 aaccgatgac tctgatttcc gaacttgctc gagccagaac agaggaggac gtcaaggacg
     9541 cgtacatcaa ggcgctcggc ctgaaatcgt atttcaaggg gctggtcgac atccagaccg
     9601 aggaggtctg gtttgaggcg aaggacgcgc caacgccgcc gatcgtgatg ttcgggcagt
     9661 tgctcgttta tgtgcgtgcg gcgaggaagc gcggcgaggc gatcccggcg ttccttgccg
     9721 tcatcgaccg cgaaaaggcg gcgattatgc ctaccgaaaa ggcgctcccg ctgctcgacg
     9781 acaagagcat cgtctggccc aagtcgggct caggcgcggg cagagaactc gccgcacaga
     9841 tcgcccccta tgttcagacg catattgtcg agtacgagat cgcgcacgac gaggcggcgt
     9901 tcgtgaaggc ggtgaaggat gcgatcagcg agcgacggat aatccgcacg ccgatcacgc
     9961 ccgataatct gcgccaggtc ttcgaccgtt ggctcgacat ggtcggcagc gagcttgggg
    10021 cggtcaaccc cgccgattat gcagtgctgt ttttcgctga cataatgcac gacggcaaga
    10081 atgaggctat gaacaatctg cccgcacggc tgctgttcag cggcgaaaag ccggtattcc
    10141 tgctcgacgg ccagacctat gaactggcaa gcgagcgcgg ctaccggaat ttctgggcca
    10201 tctatcaccg ccctcccgat cagaagcacc ggcattacct gctcgaacgg cgcgacagcc
    10261 tgctcccagt tgacgaacag aaattcaagg gcgcctatta caccccgctg cacatcgtcg
    10321 ataaagccta tgaccagctc gttgcgacgc tggggccaaa ttggcaggat cggtttatcg
    10381 tttgggacat gtgcgctggc gtcggcaatc tcgaggccaa acatagcaac ttgcgcaacg
    10441 tgtttatgtc gacgctcgac caagccgatg tcacgatcat gcgatccaat ccggcgttcg
    10501 caggcgcgac gatctttcag tacgattatc ttaacgacga cgtgactgac ttcggcgaga
    10561 tcgactacag cctatccgac aagctgccgc gggtgctgcg acaggccatc gccgacgcgg
    10621 gcgagggcaa gaagggcgcg aagccgatcc ttgtgctgat caatccacct tatgctgaag
    10681 ctgggtcgag aaccgacaaa gccataaaaa aatcggtttc gagcacaaga attggcgcat
    10741 ggatgaacgg tgcagggtac gcctacgccg cgaaagagct gtttgttcag ttcatgacca
    10801 gggctgcgaa agagctacct aatgcgtggc tcgcaatgtt cagcacactg aagtacgtta
    10861 actcagacaa ctttgagcca tttcgagcgc gttggaaggc aacctacaag ggcggcttca
    10921 taactcacag caaagcgttt gacggactga agggcaactt cccgatcggc ttcttgattt
    10981 gggacacggg tcaaaagact gaaatcgatg ccgttcccgt tcgcgtcctt gacaaggacg
    11041 ggttggagat agggtcgaaa gtgttccaca acgtgccgag ggatcaattc ctcagcaaat
    11101 ttatcgcgcg caagaaggat ttcactcatg aagtcgtacc tctcgttaat gcgatcgtac
    11161 ccactcagcg gatcgaaacg gtgcgcaata cgcattggtc gcgaggcgct gtggcgcact
    11221 tcctttgcca cggaaacgac ctccagcagg cggggacgct gacggcgatt ctttcctcaa
    11281 cgcatgacat tgggtttacc ggggcgtatt tcgtaacgga gcaaaatctc tggcaggccg
    11341 ccgtcgtctt tgccgtccgt cgtctcatca agcccacttg gctcaacgat cgtgaccagt
    11401 ttctgcaacc ctctcagcca ctcaccgacc agttcaagtc ggactgcctc gtctggatgc
    11461 tgttcaacgg cagcaacctg agcgccggag cggacgggtt gcactggaac gggcgcgact
    11521 ggtcgctggt caatcacttc ataccttaca ccgaagcgga ggtcggcgcg accggccggt
    11581 tcgagagcga cttcatggtt cgctacatgg cggatatgaa cttctcgccc gaggcgcaag
    11641 ccgtactgga cgaggggcgc aagctatgga cgcgcttcca cgagacgcaa tttccgcgaa
    11701 gaatccgcga ggactacaag ctgggcctca acgctggttg gtaccagatc cgccgtgcgc
    11761 tcgaagctta tggcgacgcg gaatggacca acttcgatcc gttcaagaca gcctacgcaa
    11821 atctaagcgc gaagttgcga cccatggttt acgaactcgg attcctgcca aggtagtaca
    11881 acctccgaga tgcgcgcgtt ccgattcccg ccgacccgtt ctgaggtcgg cggtgccgac
    11941 gccggcttgg tcgaacgggt gagctatcgt ggggctgtac ctggtctgtt ctttcgcttt
    12001 cctacagccc atcgttcgtg catgctccgc atcggctcta accggccatg ctctttgaac
    12061 cgtccgctgc ctttccgacg cgccggccgc cccaggcctt atgggcgtga ccttcgtgtg
    12121 agctttcgct gtcgcccgca acctgcggct ccacgatatt tcgcgcgaca tcgtgaactt
    12181 tatgaacttc accgccttcc tgcgccgcgc cctgccttcc cttttgcgca ccctgccgct
    12241 aaggcgtgcc aaactcacac gatcaggatt tgaatggcca aggaagaact tctggagatg
    12301 cgcgggcaag tggtcgaact gctgcccaac gccatgtttc gagtgaagct cgagaatgat
    12361 cacgagatcc tcggccatac cgccggcaag atgcgcaaga accggatccg cgtcctggtc
    12421 ggcgacgagg tgctggtcga gctgacgccc tacgacctca ccaagggtcg gatcacctat
    12481 cgcttcaaat aggccatggc agcccgcgcc ccgcggctgg tcctcgcctc cgccagtccc
    12541 aggcgcctcg aactcctcgc ccggatcggg atcgtccccg acgaggttgc cccggccgcg
    12601 atcgacgaga ccccgcgccg cggcgagctg cccgcaaact atgccgcccg gatggccgcc
    12661 gaaaaggccg ccgccgtcga acgccccggc tcgctggtgc tcgccgccga caccgtcgtc
    12721 gcggccggcc gccgcatcct gcccaaggcg gaagacgagg cgaccgcgcg cgcggcgctc
    12781 gccttgctct cgggccgccg ccaccgcgtc ctctccgccg tcactctgat cgacggcgag
    12841 ggcaaggcgc gacaccgcct ctccaccacg atcgtcacct tcaagcgcct cgcggacgaa
    12901 gatctcgacg cctatcttgc ctcgggc
//
LOCUS       DASVRK010000098         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_203566, whole genome shotgun sequence.
ACCESSION   DASVRK010000098 DASVRK010000000
VERSION     DASVRK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203566"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF589_03245"
     CDS             complement(<1..197)
                     /locus_tag="VF589_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8256622.1"
                     /translation="MAGAAKDTRGRIVEAAMELFALKGYGSTSIADILSRSQVNSGSL
                     YHYFPGKQDVLLAVLDGYRDN"
     gene            complement(278..1213)
                     /locus_tag="VF589_03250"
     CDS             complement(278..1213)
                     /locus_tag="VF589_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8256623.1"
                     /translation="MPEMRKLGGSGLETPRLILGGNVFGWTADRDRSFAILDAFVAGG
                     GNMIDTADVYSSWAPGNKGGESETIIGEWLRRRGRRDDVLIATKVGFKEGIGAAAIAA
                     GAERSLERLGIETIDLYYTHKDDPDAPLEATLGALDRLVKAGKVRAIGASQIGPERLA
                     EALDISAANGLAGYSVLQTWYNLVERPLFEGPLAEVAQERGVATVAFYSLASGFLTGK
                     YRSTADLGKSVRGGGLAKYLTPQGLAVLAALDAVAAETGATPAQVALAWIAAQPGLAA
                     PIASATSVEQVEELLGAMELKLSADQLARLGQASQ"
     gene            complement(1284..>4075)
                     /locus_tag="VF589_03255"
     CDS             complement(1284..>4075)
                     /locus_tag="VF589_03255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8256624.1"
                     /translation="APAWAAPAAPAAGLIPVESFTLPNGLKVVFHIDRSDPVVAVALN
                     AQVGSARELPGRTGFAHLFEHLFFLESENLGKGGLDKMSARIGGSGANGSTNRDMTDY
                     FQTVPNDALEKLIWAEADKLGFFINTVTDPVLAKEKQVVKNEKRQAVDNQPYGHTSAV
                     VAEALYPAGHPYSWEVIGSLKDLDAASLADVKQFYRRWYVPGNVTLVIAGDFDPKQAR
                     AWVEKYFAEIPAGAKSKPVAPRPAKLAASRSLLHEDAFAQLPELNMVWPAVPAFDEDT
                     FALQVLAKLLTDGKEAPLNAVLIDEKKLTGEVAATADLGKLSGEWWLRVRAFDGVDLD
                     AVKAAIGEGFARFERAGVDAAALERAKTALEVELYGQIGSVLGKANWIARYDIYAGDP
                     AFADRHVAAIRAVSAADVDRAYRRHIKGRPHVATSFVPKGKAQLALAGAAPAKVVEEA
                     IVQGAEAEVDAAAAAAYARTPSSFDRRVEPAAGARPEVRLPNVWQERLGNGLKLAGIA
                     DAELPVASFELAVEGGRKRDDAKRPGVAAMLARMFTRGTARRTPAELENALKSLGAEV
                     EARVEDEKFVVRGDTLARNFAATIDLVEEMLLEPRWDPAELELARAATLAEIQDERSQ
                     PDALAERAFELATYGPDSRFARSRLGTEASVKAVTMGDLKSWHAANLAPNLARFAVAG
                     AVEPPAVRQALADLGSRWPTREVAAMPFPAPPAPAKAQLFFYDVAGAKQSVFAFGYPG
                     PRRAEPDYYPAVVMNYILGGGGFASRLTQQLREGKGYTYGIRSGFSGGRELGEFRIDS
                     GVRSNATLEAAALTRDIVKDYGATFTAADLEVTKSFLTKSRARAFETQEAKLGYLGNI
                     LDHGLPADYPRREQAIVEAMTVEGVRALAGKYLRPDAMRYVIVGDAATQAGRLETLGY
                     GAPVMVKDQVAAADR"
BASE COUNT          581 a         1404 c         1420 g          670 t
ORIGIN      
        1 atattgtcgc gatagccgtc gagcaccgcc agcagcacgt cctgcttgcc cgggaaataa
       61 tggtagaggc tgccgctatt gacttggctg cggctgagaa tgtcggcgat cgaggtcgag
      121 ccgtagccct tcaatgcgaa cagctccatc gccgcctcga cgatcctgcc cctcgtgtcc
      181 ttcgccgctc ctgccatggc ggcagtgttg attgaacgtt caactaagtc aagacccacc
      241 agctcgcggc gatcggtact tcgcggcaat ggcggttcta ttgcgaagct tggccgagcc
      301 gggcgagctg gtcggcgctg agcttcagct ccatcgcccc cagcagctcc tcgacctgtt
      361 cgacgctggt ggcgctggcg atcggggcgg cgaggccggg ctgggcggcg atccaggcga
      421 gcgcgacctg ggccggcgtc gcccccgtct ccgccgccac cgcgtcgagc gcggcgagca
      481 ccgcgagccc ctgcggggtc agatatttgg cgaggccgcc gccgcggacg cttttgccga
      541 gatcggcggt ggagcggtat ttgccggtga ggaagccgct ggcgaggctg tagaatgcga
      601 cggtggcaac gccccgctcc tgcgccacct cggcgagggg cccttcgaaa agcggccgct
      661 cgaccagatt gtaccaggtc tggagcacgc tgtagccggc gaggccgttg gcggcgctga
      721 tgtcgagcgc ttcggcgagc cgttcggggc cgatctgcga ggcgccgatc gcgcgcacct
      781 tgccggcctt gaccagccgg tcgagcgcgc cgagcgtcgc ttcgagcggc gcgtcgggat
      841 catccttgtg cgtgtaataa aggtcgatcg tctcgatccc gagccgctcc agcgaccgct
      901 cggcgccggc ggcgatggcg gcggcgccga tcccctcctt gaagccgacc ttggtggcga
      961 tcagcacgtc gtcgcgccgg ccgcgccgtc gcagccactc gccgatgatg gtttccgatt
     1021 cgccgccttt gttgcctggc gcccaggacg aataaacgtc ggcggtgtcg atcatattgc
     1081 cgccgccggc gacgaaggcg tcgaggatgg cgaagctgcg gtccctgtcg gcggtccagc
     1141 cgaacacgtt gccgccgagg atcagccgcg gcgtctccag gccgctgccg ccgagtttcc
     1201 gcatttcggg catcgccgca tctcctcctg cttcgcgcgg cggcctagcc tatgcctccg
     1261 cgcgcggaaa gggattggac gcctcaccga tccgccgcgg ccacctgatc cttgaccatc
     1321 accggcgctc cgtagccgag cgtctcgagc cggccggcct gggtcgcggc gtcgccgacg
     1381 atgacgtagc gcatcgcgtc cggacgcaga tatttgcctg cgagcgcgcg caccccttcg
     1441 acggtcattg cttcgacgat cgcctgctcg cggcgcggat aatcggcggg caggccgtga
     1501 tcgaggatgt tgccgagata gccgagcttg gcctcctgcg tctcgaacgc acgggcgcgg
     1561 ctcttggtga ggaagctctt ggtcacttcg aggtcggcgg cggtgaaggt cgcgccataa
     1621 tccttgacga tgtcgcgggt cagcgccgcc gcctccagcg tcgcgttgga gcgcacgccg
     1681 ctgtcgatgc gaaattcgcc gagctcccgg ccgccactga agccggagcg gatgccgtag
     1741 gtatagccct tgccttcgcg caattgctgg gtgagccgcg aggcgaagcc gccgccgccc
     1801 aaaatgtagt tcatcaccac cgccgggtag taatccggct cggcgcggcg cggcccggga
     1861 tagccgaagg cgaagaccga ctgcttggcg ccggccacgt cgtagaagaa gagctgcgcc
     1921 ttggccggcg ccggcggcgc tgggaagggc atggcggcga cctcgcgggt cggccagcgg
     1981 ctgccgagat cggccagcgc ctgccggacc gccggcggct cgaccgcgcc cgcgaccgcg
     2041 aagcgggcga ggttgggcgc gagattggca gcgtgccagc tcttgaggtc gcccatggtc
     2101 accgccttga ccgaggcttc ggtgccgagc cggctgcgcg cgaagcggct gtcggggccg
     2161 taggtcgcca gttcgaacgc gcgttcggca agcgcgtcgg gctgcgagcg ctcgtcctga
     2221 atctcggcca gggtcgcggc ccgcgcaagc tcgagctcgg ccggatccca gcgcggctcg
     2281 agcagcatct cttcgacgag gtcgatagtg gcggcgaagt tgcgggcgag cgtgtcgccg
     2341 cgaaccacga atttctcgtc ctcgacccgc gcctccactt cggcgcccaa tgacttcagc
     2401 gcattctcga gctcggccgg cgttcgccgc gcggtgccgc gggtgaacat ccgcgcgagc
     2461 atcgccgcca cgcccggacg cttggcatcg tcgcgcttgc gccccccctc caccgccagc
     2521 tcgaagctcg ccaccggcag ctcggcatcg gcgatgccgg cgagcttgag gccgttgccg
     2581 agccgctcct gccagacgtt cggaagccgc acctcgggcc gagcgcccgc cgccggctcg
     2641 acgcggcggt cgaagctgga gggggtgcgc gcataagcgg ccgccgcggc ggcatcgacc
     2701 tcggcttcgg caccctggac gatcgcttcc tcgaccacct tcgctggcgc cgcgcccgcg
     2761 agcgccagtt gcgccttgcc cttgggcacg aagctggtgg cgacgtgcgg gcggcccttg
     2821 atgtggcggc gataggcgcg atcgacatcg gccgccgaca ccgcccggat cgcggcgacg
     2881 tgacggtcgg cgaaagcggg atcgccggca tagatatcgt agcgggcgat ccaattggcc
     2941 ttgccgagca cgctaccgat ctggccgtaa agctccactt cgagcgcggt cttggcgcgc
     3001 tcgagcgcgg cggcgtcaac gcccgcgcgc tcgaagcggg cgaagccctc cccgatcgcc
     3061 gccttgaccg catcgagatc gacgccgtcg aaggcgcgca cgcgcagcca ccattcgccg
     3121 gacagcttgc cgaggtccgc ggtcgccgcg acctcgccgg tcagcttctt ctcgtcgatc
     3181 agcaccgcgt tcagcggcgc ttccttgccg tcggtcaaga gcttcgccag cacctgcagc
     3241 gcgaacgtgt cctcgtcgaa ggcgggcacc gccggccaga ccatgttgag ctcgggcagt
     3301 tgcgcgaacg catcttcgtg gagcagcgag cggctcgccg ccagcttcgc cgggcgcggc
     3361 gccaccggct tggacttggc gccggcgggg atctcggcga aatatttctc gacccaggcc
     3421 cgcgcctgct tcgggtcgaa atcgcccgcg atgacgaggg tgacgttgcc cggcacgtac
     3481 cagcgccggt agaattgctt gacgtcggcc agcgaggcgg cgtcgaggtc cttcagcgat
     3541 ccgatcactt cccagctata ggggtggccg gcggggtaga gcgcttccgc caccacggca
     3601 ctggtgtggc cgtagggctg gttgtcgacg gcctggcgct tctcgttctt gacgacctgc
     3661 ttttccttgg cgagcaccgg atcggtgacc gtgttgatga agaagccgag cttgtcggcc
     3721 tcggcccaga tcagcttctc gagcgcgtcg ttggggacgg tctggaaata atcggtcatg
     3781 tcgcggttgg tcgagccgtt ggcgcccgag ccgccgatcc gcgcgctcat cttgtcgagc
     3841 ccgccctttc cgagattttc ggattcgagg aagaacagat gctcgaacaa atgcgcgaag
     3901 ccggtgcgcc cgggcagctc gcgcgccgac ccgacttggg cgttgagcgc caccgccacc
     3961 accgggtccg accggtcgat gtggaacacc accttgaggc cgtttggcaa ggtgaagctc
     4021 tcgaccggga tcagccccgc ggccggagcg gcgggcgcgg cccaggcggg cgcgg
//
LOCUS       DASVRK010000099         6022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_266772, whole genome shotgun sequence.
ACCESSION   DASVRK010000099 DASVRK010000000
VERSION     DASVRK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266772"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..498)
                     /gene="paaD"
                     /locus_tag="VF589_03260"
     CDS             complement(31..498)
                     /gene="paaD"
                     /locus_tag="VF589_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX8256625.1"
                     /translation="MVAAAHQTDETARIWSVLESVPDPEIPVLSVVDLGIVRELGPDR
                     VVITPTYTGCPATQVIERDIRDALDAAGYRGVRIETTLAPPWTTDWIGAEGRRKLREY
                     GIAPPRAAGRSEVECPQCGSSGTEEISRFGSTPCKAQWRCRDCLEPFDLFKCH"
     gene            complement(492..1334)
                     /gene="paaC"
                     /locus_tag="VF589_03265"
     CDS             complement(492..1334)
                     /gene="paaC"
                     /locus_tag="VF589_03265"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX8256626.1"
                     /translation="MPSLPPIRPEDEAAARRAPGAGAFDAPSELPRDVLFDMLLRLGD
                     DSLILGQRLSEWTGHAPSVEVDLSLANLALDLIGQATFFLNYAGEVEGKGRDGDRLAF
                     HRDVLDFRNCMLVEQPNGDFAQTMARQFLFSTWQKRLFEHLLGSRDDYIASVAAKALK
                     EVTYHQELASEWVIRLGDGTDESRRRMAAGFDWNWRFVPELFEMDEVADEAAARGIGA
                     DVTAFRGDYEREVRAVLAEATLEIPKEQRPILGGRRGHHSEHLGHLLCTMQYLPRAYP
                     EATW"
     gene            complement(1511..1792)
                     /gene="paaB"
                     /locus_tag="VF589_03270"
     CDS             complement(1511..1792)
                     /gene="paaB"
                     /locus_tag="VF589_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX8256627.1"
                     /translation="MNDWPLWEVFVRAKGGLSHRHVGSVHAPDAAIAVRHARDTYTRR
                     MEGVSLWVVPSAAIAASDPGDDAAMFDPAADKIYRHPTFYDIPEGITHI"
     gene            complement(1923..2924)
                     /gene="paaA"
                     /locus_tag="VF589_03275"
     CDS             complement(1923..2924)
                     /gene="paaA"
                     /locus_tag="VF589_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX8256628.1"
                     /translation="MYTTELHKSGEPRKPGKVEAIEDPGKLAAFEARVAADEFIEPKD
                     WMPEAYRRTLTRQISQHAHSEIVGMLPEGNWIGRAPSLRRKAILIAKVQDEAGHGLYL
                     YSAAETLGTSRREMIEALHSGKAKYSTIFNYPTVTWADMGAIGWLVDGAAIMNQVPLQ
                     RTSYGPYARAMVRVCKEESFHQRQGYEIMMHLAAGTPEQKHMAQDALNRWWWPSLMMF
                     GPPDADSPNTAQSMRWRIKRDTNDELRQKFVDITVPQAEAIGLTVPDPDLVWNEAKGG
                     YDFGAIDWEEFYAVVRGEGPVAKERMKARREAWDDNAWVREAADAYAAKHARKAEAA"
     gene            complement(3048..4244)
                     /locus_tag="VF589_03280"
     CDS             complement(3048..4244)
                     /locus_tag="VF589_03280"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8256629.1"
                     /translation="MPRLLLPICMLILSACVSAPPPSPSPPGVSYAWVAFDASRVRGS
                     GASGLADRARARPLTVEDPVRIASISKLVVALGAMRLVEQGKLDLDADVSGALGWRLR
                     NPAFPDTPITLRLLLSHRSSIRDDVDYAIPLGGTVRATLADPKAFDPDHAPGRFFRYS
                     NLNFPVAATVMEKVTGERFDRLMERLVLDPLGLDACFNWTTCSDAALARAVVLYAPDG
                     SVIRDDLGGEWPACPVVQAADGSCDLARYQPGENGALFSPQGGLRISARDLATVGQLL
                     LNRGRHRSAPFLSQASLAALTGEAWRFDGANGAPEDGFYCSYGLAVQRLPVRAPGCRD
                     DLTGGGRGLIGHAGEAYGLRSGLWVDPKRGVGVAYFAVNNGDAPPPGRSAYRAIEERL
                     AARLPR"
     gene            complement(4726..5274)
                     /gene="grpE"
                     /locus_tag="VF589_03285"
     CDS             complement(4726..5274)
                     /gene="grpE"
                     /locus_tag="VF589_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX8256630.1"
                     /translation="MNDDQNLKDEAEEIREDTAAEAPELKEHDRLGALEKELADARQQ
                     VLYAQAETQNVRRRLEAEKLAASAYAATGFARDMLSVKDNLDRALAAFPEELRQDDRI
                     RALLTGIEATSRELDNVFGRNGITRIEAMGQPLDPNRHQAMLEVPDEAAEPGTIVQEM
                     QAGYMIKDRLLRPALVGVARKA"
     gene            complement(5271..>6022)
                     /gene="hrcA"
                     /locus_tag="VF589_03290"
     CDS             complement(5271..>6022)
                     /gene="hrcA"
                     /locus_tag="VF589_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126640.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX8256631.1"
                     /translation="IEGQLGRNGPIEEALAAATAALSGLSACAGIVLVPKREPVLRQL
                     GFVQLSATQALAVMVGGDGSVENRVIDLPPGVTPAALAEVGNYVSARLSGLTLHEAQA
                     RLAEEIGAGRAALDRASQELVARGLAVWSEDGASRPVLIVRGQANLIDQAAAADLERV
                     RQLLDEIEQRQEIARLLDSARAGQGMKIFIGSENKLFALSGSSVIAAPYRGGDGKVVG
                     VVGVIGPTRLNYARVVPMVDFTAQALSRLMA"
BASE COUNT          954 a         2025 c         2081 g          962 t
ORIGIN      
        1 attcggccgc tccacgggga tgttgccgga tcaatggcat ttgaacaggt cgaacggctc
       61 gaggcagtcg cggcagcgcc attgcgcctt gcagggggtc gagccgaacc ggctaatctc
      121 ttcggtcccg gacgagccgc attgcgggca ttcgacctcg ctccgcccgg ccgcccgcgg
      181 cggcgcgatg ccatattcgc gcagcttgcg cctgccctcc gcgccgatcc agtcggtcgt
      241 ccagggcggc gcgagcgtgg tttcgatgcg gacgccgcgg tagccggcgg cgtcgagcgc
      301 gtcgcgaatg tcgcgctcga tcacctgggt ggcggggcag ccggtgtaag tgggcgtgat
      361 caccacccgg tcggggccga gttcacggac gatgccaaga tcgaccaccg aaagcactgg
      421 aatctctgga tcgggaaccg attccagcac cgaccagatg cgggcggtct catcggtttg
      481 gtgtgcggcc gctaccatgt cgcctcggga taggcgcggg ggagatattg catggtgcag
      541 aggagatggc cgagatgctc gctatggtgg cctcggcggc cgccgaggat cgggcgctgc
      601 tccttaggta tttcgagcgt ggcttcggcc agcacggcgc ggacctcgcg ctcataatcg
      661 ccgcggaagg cggtgacgtc ggcgccgatg ccgcgggcgg cggcttcgtc ggcgacctca
      721 tccatttcaa acagttccgg cacgaagcgc caattccagt cgaacccggc ggccatgcgg
      781 cggcggcttt cgtcggtgcc gtcgccgagc cggatcaccc attcgctggc gagctcctga
      841 tgataagtga cttccttcag cgccttggcg gcgacgctgg cgatataatc gtcgcgcgag
      901 ccgagcagat gctcgaacag ccgtttctgc caggtcgaga acaggaattg ccgcgccatg
      961 gtctgggcga agtcgccatt gggctgctcg accagcatgc agttgcgaaa atcgagcacg
     1021 tcgcggtgaa aggcgagccg gtccccgtcc cggcccttgc cctcgacctc gccggcatag
     1081 ttcaggaaga aggtcgcctg gcctatgagg tcaagcgcca gattggcgag gctgaggtcg
     1141 acttcgaccg agggggcatg gccggtccat tccgaaagcc gctggccgag gatcaggctg
     1201 tcgtcgccga gccggagcag catgtcgaac agcacgtcgc gcggcagctc cgacggagcg
     1261 tcgaacgcgc cggcgccggg ggcacgacgg gcggcggctt cgtcttccgg gcgaatgggt
     1321 gggagcgagg gcatctagag ggaaaatccg agcgcgtggc tggaagcgag ggcccttgcc
     1381 aaacaccgtt cgccctgagc ctgtcgaagg gtcttcctcg ccttccttcc gaaaaaggag
     1441 agggcttcga caggctcagc ccgaacgggt gaggaggccg gtgcgtttcc cccgatgcgc
     1501 gaatcgcccc tcatatatgc gtgatccctt cggggatgtc gtagaaggtc gggtggcggt
     1561 agatcttgtc ggcggcggga tcgaacatgg cggcgtcgtc gcccggatcg gaagcggcga
     1621 tcgcggccga gggcaccacc cacaggctca ccccctccat ccggcgcgta taggtgtcgc
     1681 gggcatggcg gacggcgatc gcggcgtcgg gcgcgtggac cgacccgaca tggcggtgcg
     1741 acagtccgcc cttggcgcgg acgaacactt cccagagcgg ccagtcgttc attgtatcct
     1801 ccccgggacg aggaggggga ccgcggcgcg aagcggcgtg gtggaggggg cccggagcgg
     1861 ggcacggggc cccctccacc agcctgcggc tggtccccct ccccgttccg gggaggaact
     1921 catcatgccg cctccgcctt gcgggcatgc ttggcggcat aagcgtcggc cgcctcgcgg
     1981 acccaggcat tgtcgtccca ggcctcgcgg cgggccttca tccgctcctt ggcgaccggg
     2041 ccctcgccgc ggacgacggc gtagaattcc tcccaatcga tcgcgccgaa atcatagccg
     2101 cccttggcct cgttccagac gaggtccgga tcgggcacgg tgaggccgat cgcttccgcc
     2161 tgcggcacgg tgatgtcgac gaacttctgc ctgagctcgt cattggtgtc gcgcttgatc
     2221 cgccagcgca tcgactgggc cgtgttgggg ctgtcggcgt cgggcgggcc gaacatcatc
     2281 agcgacggcc accaccagcg gttcaaggcg tcctgcgcca tatgcttctg ctcgggcgtg
     2341 ccggcggcga gatgcatcat gatctcatag ccctggcgct ggtggaagct ctcctccttg
     2401 cagacccgca ccatcgcgcg ggcataaggg ccgtaggagg tgcgctggag cggcacctgg
     2461 ttcatgatcg ccgcgccgtc gaccagccag ccgatcgcgc ccatgtcggc ccaggtgacg
     2521 gtggggtagt tgaagatggt gctgtacttg gccttgcccg agtggagcgc ctcgatcatc
     2581 tcgcggcgcg aggtgccgag cgtctccgcg gccgaataga gatagaggcc gtggccggcc
     2641 tcgtcctgga ccttggcgat caggatcgcc ttgcgccgga gcgagggggc gcggccgatc
     2701 caattgcctt cgggaagcat gccgacgatc tcgctatgcg cgtgctgcga gatctgacgg
     2761 gtgagcgtgc ggcgataggc ctcgggcatc cagtccttgg gctcgatgaa ctcgtcggcc
     2821 gcgacccgcg cctcgaaggc ggcgagcttg ccgggatcct cgatcgcctc gaccttgccg
     2881 ggcttccgcg gttcgccgct cttgtgcaat tcggtcgtgt acatggcttg gctcctcggc
     2941 gccgccgata tcattaaccg accaagcggt caacgaattc gggcgggcgc tttcactgcc
     3001 accaacgcgc gagccggctt gtggtggcgg atcgctccac ctcgcggtca gcgcggcagg
     3061 cgcgcggcca gcctctcctc gatggcgcga taggcggagc ggccgggggg cggagcgtcg
     3121 ccattgttga ccgcgaaata agcgacgccg acgccgcgct tcgggtccac ccagaggccg
     3181 gagcgcaggc catacgcctc accggcatgg ccgatgaggc cgcggccgcc gccggtcagg
     3241 tcgtcgcggc agccgggcgc ccgcaccggc aggcgctgca cggcaaggcc gtagctgcag
     3301 taaaagccgt cctcgggcgc gccattggcg ccgtcgaagc gccacgcttc gccggtgagg
     3361 gcggcgaggc tcgcttggct gaggaacggc gcgctgcggt ggcggccgcg gttcagcagc
     3421 agctgcccga cggtggcgag atcgcgggcc gagatgcgca gtcctccctg cggcgagaac
     3481 aaagcgccat tctcgccggg ctggtagcgg gcgaggtcgc agctcccgtc ggcggcctgc
     3541 acgaccgggc aggccggcca ctccccgccg agatcgtcgc ggatcacgct gccgtcgggg
     3601 gcgtagagca ccaccgcgcg ggcgagcgcg gcatcgctgc aggtggtcca gttgaagcag
     3661 gcgtcgaggc cgagcggatc cagcaccagc cgctccatca gccggtcgaa ccgctcgccg
     3721 gtgaccttct ccatcaccgt cgcggcgact gggaagttga ggttcgaata gcggaagaag
     3781 cggccggggg cgtgatcggg atcgaaggct ttgggatcgg cgagggtggc gcggacagtg
     3841 ccgccgagcg ggatcgcgta atcgacgtcg tcacggatgc tcgatcgatg cgagagcagc
     3901 agccgcagcg tgatcggcgt gtcggggaac gccgggttgc gcagccgcca gccgagcgcg
     3961 cccgacacgt ccgcatcgag atcgagcttc ccctgctcca ccagccgcat cgcgcccagc
     4021 gccaccacca gcttggagat ggacgcaatc cgcaccggat cctcgaccgt cagcggcctc
     4081 gcgcgggctc ggtctgcgag gcctgaggcc ccgctgccgc ggactctcga ggcgtcgaag
     4141 gccacccagg cgtagcttac gcctggaggc gatggcgatg gtggaggtgc gctgacgcag
     4201 gcgctcaaga tgagcatgca gatcggaagg agcaatctcg gcatcgcccc cccattcacc
     4261 ggttttcaac gcccgggcaa tagaagccaa tcatgctgca cgagatcgtc gcccaccatc
     4321 cgattggcga aacgccttac aacaagctga tctacgtggg tgcaaactgc cacggcaccc
     4381 cactttttca ggtgatgggg acgagccgaa caccctgctc agcccccact gcgctgagag
     4441 cagcgttcat acggtacggc gggcactgct actattgcgg cacgcggctg aagcctcagg
     4501 caaggccgag ccccatgagc atcgaccacg tcataccgaa ggctctcggc ggcacaaacc
     4561 tgcttcacaa cttcgtgatc gcgtgcaagc cttgcaataa gagcaagggc tctcaggcga
     4621 tacttgagta caagcttgag gcgggtaatg cttacctcac cgccctggaa cgtcacattg
     4681 ccgacgcagt gcggagcgcc gctgaacccg gcagcgcccc ctcccctacg ccttccgcgc
     4741 caccccaacc aaagcggggc gcagcagccg gtccttgatc atgtagccgg cctgcatctc
     4801 ctggacgatg gtgccgggct cggccgcctc gtcggggact tcgagcatcg cctggtggcg
     4861 gttggggtcg agcggctggc ccatcgcctc gatccgggtg atgccgttgc ggccgaacac
     4921 attgtcgagc tcgcgcgaag tcgcctcgat gccggtgagc agcgcccgga tgcggtcgtc
     4981 ctggcgcagc tcctccggaa aggcggcgag cgcgcggtcg agattgtcct tcacgctcag
     5041 catgtcgcgg gcaaagccgg tggcggcgta agcggaagcg gcgagcttct ccgcctcgag
     5101 ccggcggcgg acgttctgag tctcggcctg cgcatagagc acctgctggc gcgcgtcggc
     5161 gagctccttt tcgagcgcgc ccaaacggtc atgctccttg agctcgggcg cctcggcggc
     5221 ggtatcctcg cggatctcct ccgcctcatc cttcagattc tggtcgtcgt tcatgccatc
     5281 aatctcgata gtgcctgtgc cgtgaaatcc accatgggca cgacccgcgc atagttcaac
     5341 cgggtcgggc cgatcacgcc gaccacgccg accaccttgc cgtcgccgcc gcgatagggc
     5401 gcggcgatca ccgacgagcc ggacaaagcg aacaatttat tctccgatcc gatgaagatc
     5461 ttcatcccct gcccggcccg cgcgctgtcg agcaggcggg cgatctcctg gcgctgctcg
     5521 atctcgtcga gcaactgccg gacccgctcg agatcggcgg cggcggcctg gtcgatgaga
     5581 ttggcctggc cacgcacgat cagcaccggc cgcgaggcgc cgtcctccga ccagaccgcg
     5641 agcccgcgcg cgaccagttc ctgcgaggca cggtcgagcg ccgcccgccc ggcgccgatc
     5701 tcctcggcga gccgcgcctg ggcctcgtgc agggtcagcc cggacagccg cgcgctgaca
     5761 taattgccga cctcggcgag cgcggccgga gtcaccccgg gcggcaggtc gataacgcga
     5821 ttctcgacgc tgccgtcgcc gccgaccatc accgccagcg cttgggtggc ggagagctgg
     5881 acgaagccga gctggcgcag caccggctcg cgcttgggca ccagcacgat gccggcgcag
     5941 gccgacaggc ccgacagagc cgcggtcgcc gctgccagcg cctcctcgat cggcccgtta
     6001 cggccgagct gcccctcgat cg
//
LOCUS       DASVRK010000100        10005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_453297, whole genome shotgun sequence.
ACCESSION   DASVRK010000100 DASVRK010000000
VERSION     DASVRK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453297"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VF589_03295"
     CDS             <1..518
                     /locus_tag="VF589_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEX8256632.1"
                     /translation="ASDKYIIGPLDELTVFVWRNPELGAKVQVRPDGRITTPLIADMQ
                     AVGKTPKQLSEDIKRLLVKYIEDPLVSVMVDRFQGTFSQQVRIVGATEKPASLPYRAN
                     MTLLDAMIEVGGLSEFAAGDKARLVRQDRATGRQKEYDLKISRLIKKGDTTANVKLEP
                     GDVIIIPESMF"
     gene            557..2020
                     /locus_tag="VF589_03300"
     CDS             557..2020
                     /locus_tag="VF589_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX8256633.1"
                     /translation="MDGLYEQIRIALHQIRRRRWLALAVAWGLCMAGWLGIALIPNSY
                     ESKARVFAQMQSILPDKIGITAAERQNDLLQIKQMLASTENLQKVVRRTDLNQQVRSE
                     RDLAGQVEKLRENIKITAQIDNANVIEISATSSLSGFSNAQNAKTAAAIVQNLLDLFI
                     EENLSGDRAETGQTLGFLDEELRRREAQLHAAEQRRVDFETRYMGLLPGEGSIEQRMG
                     AARSELANVEQQLLTAQSALSAIRSQMGSTPASVATPGFSGGGGYAAAQIARLRPQAE
                     TEANAAPAPGSTSPNPAYASMRAMAAEREAQAAAASARKNQIQSAMLQLSQKQSAEPE
                     AAAEQARLNRDYEVLKRQYDKLLEDREQVRLRSDVQSKTDAINFRIIDPPSKPTIPVA
                     PNRPLLLSLILVVAIAGGVAAAFTKGQLQMTFPTQGRLEQATGLPVLGSVSEVLTAAA
                     KAERKQRMKWFAGAGGALAASYGILMLVEFWQRSTVA"
     gene            2032..3141
                     /locus_tag="VF589_03305"
     CDS             2032..3141
                     /locus_tag="VF589_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEX8256634.1"
                     /translation="MSKHQAFSRRPSLLERAAEIYDFSSAAPLAPAPAPQPEARPEPK
                     PQPRIVRVEQQAEVAPEPVRRPAPAPAAPAWDALEEAAPEPFAEVPAEAEIAELVLTE
                     PAAAAPSAPPAPRPARAKRQVALPARYGTVDRAALREGEFILPDAPTTALAEEFRIIK
                     RQLLLRAAESASTEPRRRQTILVCSAQPDEGKTFCALNLALSLATEKDLEVLLVDGDF
                     NKPEILSLLGLENGPGFIDAIADPAAEVNDFVVRTDIDGLSVLPAGRQTNNITELLAS
                     ERTREVLDALTGPHPRRIVVFDSPPALMASPASVLATHVGQVMVVVRADQTHEADLKE
                     AVGLLSGCDTLSLLLNGAGFAATGRRFGSYYGYGQ"
     gene            3138..4787
                     /locus_tag="VF589_03310"
     CDS             3138..4787
                     /locus_tag="VF589_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256635.1"
                     /translation="MIELASLKRAGIGALGLAGLAAGIALAATASSARTDITPYLEVQ
                     QVLTADLNDGGDVLTYTSAAAGVNGSISTQRLEAQISYRYERRIAYNEDLFDDEVHSG
                     IAQAHYQIVPGKVTLDGGAMAARSRADGRGPIFGFSSFDNQNVSEVYSVYAGPTLSTK
                     VGEIDVGAQYQVGYVKVDDHGLAGLPSVPGQEILDRYDSSISQNVSASVGMGPGRFPV
                     GWTVGVGYVREDVNRLDQAFEGKYIRGDVVFPVTPTVALTAGVGYEKMQSEQDDLKRD
                     AAGVPILTPGGNLIADPSKPRLLTYDQSGVIWDAGVIWRPSRRTELQARFGRRYGGTT
                     ITGSFRHEFNSALAIQANVVDSVSSFGRLLVDDLNGLPVDFNPNRNPLSPGIGGVGGC
                     VFGREPGTGSCFDNAFQSINTNNFRMRGGNVLVSGGRGPWSLGAGAGYSRRKYLAPAI
                     VAGEFDMDNVVDESFTLSGFASRELSRTSAVNLDVYAAWFDSSAPFSETAFSSGISGS
                     YNQSIFNERLQGYAAVGLYTTDSGDFDSTGVTGLLGLRYTF"
     gene            4823..5869
                     /locus_tag="VF589_03315"
     CDS             4823..5869
                     /locus_tag="VF589_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HEX8256636.1"
                     /translation="MYTAFYGLSDRPFQLTPDARFYFDSRTHKKAMAYLGYGLAQGEG
                     FIVITGEVGAGKSTLVAHLMATIDRARLNAVSIVSTQVEGDDMLRLAAQKLGIETEGV
                     AKARLLDLVEERLEEEARSGKRSLLIVDEAQNLPHSALEELRMLSNFTSGGRALLQIF
                     LLGQPEFRDAVASPALEQLRQRVIATHHLEPMEANEVESYMLHRLALVGWEDRPRFDE
                     AAFEAIYRHTDGIPRRVNQLASRILLFAAMEQLDAIDAEAVEAVAADMASDSPQERVL
                     PLRVSPQPAAAAPSFERRSPPEPVADFVLERRIASLEARIEEQEHAVRRVLGLLIDWV
                     ESDQAPLSYRHNAA"
     gene            5879..6724
                     /locus_tag="VF589_03320"
     CDS             5879..6724
                     /locus_tag="VF589_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HEX8256637.1"
                     /translation="MINALSVDVEDWFQVGAFENVIARDSWDGLAHRVERNGDAVLAL
                     FDQARVKGTFFTLGWVAQRYPALVRRIAEAGHEVASHGWDHRRVFTLSAAEFRADLER
                     ARIAIEDACGHSPAGYRAPSFSIDARTPWAHRVLAEEGYAYSSSVAPVRHDHYGWPEA
                     PRFAFRPVEGERLIELPVTTVEVAGRRLAAGGGGFFRLLPYRFSSWAVGRVNRVEQRP
                     AIFYFHPWEIDPGQPRVEAAPLRSRLRHYTNLSAMQPKLLKLLKAHTWGRTDHVVAAE
                     RERLA"
     gene            6721..7785
                     /locus_tag="VF589_03325"
     CDS             6721..7785
                     /locus_tag="VF589_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEX8256638.1"
                     /translation="MNAFSPVAGVSIRTVELAPNRDRIDAYVVAQAGAQPFHRPQWSE
                     AVERGCGQRAYYLVAERPSRALAGVLPLTEIRSSLFGTALVSAGFAVGGGPLAEDDTV
                     AEALAKAAWNLTRQLGCPSIEMRGGRVPLGWKRCEGVYAGFVRALPAGDEAILTAIPR
                     KQRAEVRRAQGFGLDVSHGSSASDRDAHYSVYAESVRNLGTPVFPRELFGAMLDAFGE
                     DADIVTVWKDGVPVSSVLSFYLNGTVYPYWGGGTRAARTLRANELAYYELMRRAAARG
                     CDRFDFGRSKLGTGAFAFKKNWGFEPQPLVYSMRTAPGMTPREINPLSPKYRLKVAAW
                     QRLPLWIANRLGPRIARGLG"
     gene            7785..9011
                     /locus_tag="VF589_03330"
     CDS             7785..9011
                     /locus_tag="VF589_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HEX8256639.1"
                     /translation="MTDILFLAHRIPYPPDRGDKIRSWNMLKFLAGLGRVHLACFADD
                     PKDAAHLAGLKAELGSAVGRTHVELRSTGRIAAALQALRTGGPVSLPLFGSQRMQGFV
                     DEILASEPVGTIFAFSGQMAQFVPAGAPQRFVMDFVDMDSAKFADYAREGGGPSAWIH
                     AREAGKLFAFERATAARADLSLFVSEAEAGLFRAEARLPGADIRAIQNGVDLGYYDPA
                     ADFPRLAPFAGPLIVFTGQMDYRPNVEAVSAFTAESFPAIRRAHPDAVFAIVGRSPAD
                     SVRRLQQRPGIVVTGAVPDVRSWLAAADVVVAPLRIARGIQNKVLEAMAMARPVVASP
                     PAFEGIEAESGRDLVVADTPAAEAEAVLALLADRDRAAGLGRAARLRMEEAYRWQARL
                     APLAAILAQPRRKAAA"
     gene            9008..>10005
                     /gene="xrtA"
                     /locus_tag="VF589_03335"
     CDS             9008..>10005
                     /gene="xrtA"
                     /locus_tag="VF589_03335"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEX8256640.1"
                     /translation="MTAHTPIAATATAPAAAISGWRLHLTGLAAAAVAMLALFHRDVV
                     DIVDLWWNSSTYNHCLLILPIVAWLVTQRLPELRGLTPAAWAPGLLLVAAGAGGWLLG
                     EAGGVGLARHGGIVLMLQGAVIACLGKAVSRGLAFPIFYALFLIPAGEELVPPMQTLT
                     ADMCMALLALVGVPAHIEGIFITTPSGYFEVAEACSGVKFLVAMIAYGALVANVCFKS
                     AWRRAAFMLAAIVIPILANGVRAWGTIYIAEYTGTDFAAGFDHVVYGWVFFAIVIALL
                     MAAGWRFFDHKPNDPWFDPAALQAEGEPAGPANRLVIVALAAAFTAALPLGWSAAVA"
BASE COUNT         1520 a         3644 c         3258 g         1583 t
ORIGIN      
        1 atgccagcga caaatacatc atcggccccc tcgacgagct caccgtgttc gtgtggcgca
       61 atccggagct gggcgcgaag gttcaggtcc gccccgacgg ccgcatcacc acgccgctga
      121 tcgccgacat gcaggcggtc ggcaagacgc ccaagcagct ttccgaggac atcaagcggc
      181 tcctggtcaa atatatcgaa gaccctttgg tctcggtgat ggtcgatcgc ttccagggca
      241 ccttctcgca gcaggtccgc atcgtcggcg ccaccgagaa gccggcctcg cttccctacc
      301 gcgccaacat gaccttgctc gacgcgatga tcgaggtcgg cggcctttcc gaattcgccg
      361 ccggcgacaa ggcgcggctg gtccgccagg accgggcgac cggccgccag aaggaatatg
      421 acctcaagat ctcgcggctg atcaagaagg gcgacaccac cgccaacgtc aagctcgagc
      481 ccggcgacgt catcatcatc cccgagagca tgttctgagc cgcccccggc gcactcccta
      541 aggatcgcga aagccgatgg acggacttta cgaacaaatt cggatcgccc tgcaccagat
      601 ccggcgccgc cgctggctcg cgctggcggt tgcctggggc ctgtgcatgg ccggttggct
      661 aggcatcgcg ctgatcccga acagctacga atcgaaggcg cgcgtgttcg cgcagatgca
      721 gtcgatcctg cccgacaaga tcggcatcac tgccgccgag cggcagaacg atctgttgca
      781 gatcaagcag atgctggcct cgaccgagaa cctccagaag gtggtgcggc ggaccgacct
      841 caaccagcag gtgaggagcg agcgcgatct cgccggccag gtcgagaagc tgcgcgagaa
      901 tatcaagatc accgcccaga tagacaatgc caacgtcatc gagatcagcg cgacctccag
      961 cctcagcggc ttttccaacg cgcagaacgc caagaccgcg gcggcgatcg tccagaatct
     1021 gctcgacctg ttcatcgagg agaatctctc cggcgaccgc gccgagaccg gccagacttt
     1081 gggcttcctc gacgaggagc ttcgccggcg cgaggcccag cttcacgccg ccgagcagcg
     1141 ccgcgtcgat ttcgagaccc gctatatggg cctgctgccg ggcgaaggct cgatcgagca
     1201 gcgcatgggc gcggcccggt ccgagctcgc caatgtcgag cagcaattgc tgaccgcgca
     1261 gagcgcgctg tcggcgatcc gcagccagat gggcagcacg ccggcgagcg tcgccacgcc
     1321 cggcttcagc ggcggcggcg gctatgcggc cgcgcagatc gcgcggctcc gcccgcaggc
     1381 cgagaccgag gcgaacgccg ccccggcgcc cggctccacc tcgcccaacc cggcttatgc
     1441 ctcgatgcgc gccatggccg ccgagcgcga agcccaggcc gccgccgcca gcgcccgcaa
     1501 gaaccagatc cagtcggcga tgctgcagct ctcgcagaag cagtcggccg agcccgaagc
     1561 ggcggccgag caggcgcggc tcaaccgcga ttatgaagtg ctgaagcgcc aatatgacaa
     1621 attgcttgag gatcgcgagc aggtccgcct gcgcagcgac gtccagagca agaccgatgc
     1681 gattaacttc cggatcatcg atccgccgtc caagccgacc atcccggtcg cgccgaaccg
     1741 gccgcttctg ctgagcctga tcctggtggt cgcgatcgcc ggcggcgtcg ccgcggcctt
     1801 caccaagggc cagctgcaga tgaccttccc gacccagggt cggctcgagc aggcgaccgg
     1861 cctgccggtg ctcggctcgg tgagcgaagt gctgaccgcg gccgccaagg ccgagcgcaa
     1921 gcagcggatg aaatggtttg ccggcgccgg cggcgcgctg gcggcgagct acggcatctt
     1981 gatgctggtc gaattctggc agcgcagcac ggtggcgtga gggacatgga catgagcaag
     2041 catcaggctt tcagccgccg tccctcgctc ctcgagcggg ccgcggagat ttacgacttc
     2101 agctccgccg ccccgctcgc gccggcaccg gcgccgcaac ccgaggcccg gccggagccg
     2161 aagccgcagc cgcgcatcgt gcgcgtcgag cagcaggccg aggtcgcgcc cgagccggtt
     2221 cgccggccgg cgccggcgcc cgccgctccc gcctgggacg cgctggagga agccgcgccc
     2281 gagccctttg ccgaagtccc ggccgaagcg gaaattgccg agctggtcct gaccgagccg
     2341 gcggcggcag cgccgtccgc gccgcccgcg cccaggccgg cccgcgcgaa gcgccaggtg
     2401 gcgctccccg cccgctatgg caccgtcgat cgcgccgcac tccgggaagg cgagttcatc
     2461 cttcccgatg cgccgacgac ggcgctcgcc gaggaattcc gcatcatcaa gcgccagctg
     2521 ctgctgcgcg cggccgaatc cgcgtccacc gagccgcgcc gccgccagac catcctggtc
     2581 tgctcggccc agccggacga gggcaagacc ttctgcgcct tgaacctcgc tctgtcgctg
     2641 gcgaccgaga aggacctcga agtgctgctg gtcgacggcg acttcaacaa gcccgagatc
     2701 ctctcgctgc tcggcctcga aaacggcccc ggcttcatcg acgcgatcgc ggatccggcc
     2761 gccgaggtga acgacttcgt ggtccgcacc gatatcgacg ggctgtcggt gcttccggcc
     2821 ggccgccaga ccaacaacat caccgaattg cttgcgtccg agcgcacccg cgaggtgctc
     2881 gacgcgctca ccgggccgca cccgcggcgg atcgtcgtgt tcgattcgcc gcccgcgctg
     2941 atggcctcgc ccgcctccgt cctcgccacc catgtcgggc aggtgatggt ggtggtgcgg
     3001 gccgaccaga cccacgaggc cgacctcaag gaagcggtcg gcctgctttc cggctgcgac
     3061 actctcagcc tcctgctcaa cggagccggc tttgcagcca ccggccgccg gttcggcagc
     3121 tattatggat atggccaatg atcgagcttg cctccctgaa gcgcgccggc atcggcgcct
     3181 tgggcctcgc cggcctcgcc gccggcatcg cccttgccgc gaccgcctcc tcggcgcgga
     3241 ccgacatcac gccgtacctc gaggtccagc aggttctcac cgccgacctc aacgatggcg
     3301 gcgacgtgct cacctacacc tccgccgcgg cgggcgtgaa cgggagcatc agcacccagc
     3361 ggctcgaggc gcagatcagc taccgctacg agcggcggat cgcctataac gaggatctgt
     3421 tcgacgacga agtccatagc ggcatcgccc aggcccatta ccagatcgtg cccggcaagg
     3481 tgacgctcga cggcggcgcc atggcggcac gctcgcgcgc cgacggccgc ggcccgatct
     3541 tcgggttcag cagcttcgac aaccagaatg tgtcggaagt ctacagcgtc tatgccggcc
     3601 cgaccctctc gaccaaggtc ggcgagatcg acgtcggcgc gcaatatcag gtcggctacg
     3661 tcaaggtcga cgatcacggc ctcgccggcc tgccgagcgt tccggggcag gagatactcg
     3721 accgctacga cagctcgatc agccagaatg tcagcgccag cgtcggcatg ggcccgggcc
     3781 gcttcccggt cggctggacg gtcggcgtcg gttatgtccg cgaggacgtc aaccggctcg
     3841 atcaggcctt cgagggcaaa tatatccgcg gcgacgtcgt ctttccggtg actccgaccg
     3901 tggccctcac cgccggtgtc ggctatgaga aaatgcaatc ggaacaggat gatctgaagc
     3961 gtgatgcagc cggcgtgcca atcttgacgc cgggcgggaa cctgatcgcc gatccctcca
     4021 agccgcggct cctcacctac gaccagagcg gcgtcatctg ggatgccggc gtgatctggc
     4081 ggccgagccg ccgcaccgag ctccaggccc ggttcgggcg ccgctatggc ggcaccacca
     4141 tcaccggctc gttccgccat gaattcaact cggcgctcgc catccaggcc aatgtggtcg
     4201 acagcgtgtc gagcttcggc cggctgctgg tcgacgacct caacgggctg ccggtcgact
     4261 tcaatcccaa ccgcaacccg ctgagccccg gcatcggcgg cgtcggcggc tgcgtgttcg
     4321 gccgcgagcc gggcaccggc agctgcttcg acaacgcctt ccagtcgatc aacaccaaca
     4381 atttccggat gcgcggcggc aacgtcctcg tctccggcgg ccgcggcccc tggtcgctcg
     4441 gcgccggcgc cggctattcg cggcgcaaat atctggcgcc cgccatcgtc gccggcgagt
     4501 tcgacatgga caatgtcgtc gacgagagct tcacgctgag cggcttcgct agccgcgagc
     4561 tcagccggac ctcggcggtc aacctcgacg tctatgccgc atggttcgac agcagcgcgc
     4621 cgttcagcga gaccgccttc agcagcggca tcagcggcag ctacaaccag tcgatcttca
     4681 acgagcggct gcagggctat gcggcggtcg ggctctacac caccgactcc ggcgatttcg
     4741 acagcaccgg cgtcaccggc ctgctcgggc tcagatacac tttctaatcg caagggcggg
     4801 ggcgccccac ggagaatagc tgatgtacac cgccttttac ggactgagcg accggccgtt
     4861 ccagctcacg cccgacgcgc gcttctattt cgacagccgc acccacaaga aggccatggc
     4921 ctatctcggc tacggccttg cccaagggga aggcttcatc gtcatcaccg gcgaggtcgg
     4981 cgccggcaag tccacgctcg tcgcccattt gatggcgacg atcgaccgcg cgcgcttgaa
     5041 cgcggtcagc atcgtctcga cccaggtcga aggcgacgac atgctccgcc tcgccgccca
     5101 gaagctcggc atcgagacgg aaggcgtcgc caaggcgcgg ctgctcgacc tcgtcgagga
     5161 gcggctcgag gaggaggcgc gcagcggcaa gcgcagcctg ctgatcgtcg acgaggcgca
     5221 gaacctgccc cattcggcgc tcgaagagct gcgcatgctc tccaacttca cctcgggcgg
     5281 ccgcgctttg ctccagatct tcctgctcgg ccagcccgaa ttccgcgacg cggtcgcctc
     5341 gccggcgctc gagcagcttc gccagcgggt catcgccacc caccatctcg agccgatgga
     5401 ggcaaacgag gtcgaatcct acatgctcca ccggctggcg ctggtcggct gggaggaccg
     5461 tccgcgcttc gacgaggccg cgttcgaggc gatctaccgc cacaccgacg gcattccccg
     5521 ccgcgtcaac cagctcgcca gccgcatctt gctgttcgcg gcgatggagc agctcgacgc
     5581 catcgatgcc gaagcggtcg aggccgtggc cgccgacatg gcgtcggatt cgccgcagga
     5641 gcgcgtgctt cctttgcgcg tgtcgccgca gcccgccgcc gccgctccct ccttcgagcg
     5701 gcgctcgccg ccggagccgg tcgccgattt cgtgctggag cgccgcatcg cctcgctgga
     5761 agcgcggatc gaggagcagg aacatgcggt ccgccgcgtg ctcggcctgc tcatcgactg
     5821 ggtcgaaagc gatcaggcgc cgctgagcta ccgccataat gcggcttgag gccgctttat
     5881 gatcaacgcc ttatcggtcg atgtagagga ctggttccag gtcggcgcgt tcgagaacgt
     5941 gatcgcccgc gatagctggg acggcctcgc gcaccgcgtc gagcgcaacg gcgacgccgt
     6001 gctggcgctg ttcgaccagg cccgggtcaa gggcaccttc ttcacgcttg gctgggtggc
     6061 gcagcgctat ccggccctgg tccggcgcat cgccgaggcc gggcatgaag tcgccagcca
     6121 cggctgggac catcgccggg tgttcacgtt gagcgcggcc gagtttcgcg ccgacctcga
     6181 acgcgctcgc atcgccatcg aggacgcctg cggccactcg cccgccggct accgggcgcc
     6241 gagcttctcg atcgacgccc gcacgccctg ggcgcatcgc gtcctcgccg aggaaggcta
     6301 tgcttattcc tccagcgtcg cgcctgtccg ccacgaccat tatggctggc cggaagcgcc
     6361 gcgcttcgcc ttccgcccgg tcgaaggcga gcggctgatc gagcttcccg tcaccaccgt
     6421 ggaagtcgcc ggccgccgcc tcgccgccgg aggagggggc ttcttccgct tgcttcccta
     6481 tcgtttttcg agctgggccg tcggccgcgt caatcgcgtc gagcagcggc ccgccatctt
     6541 ctatttccac ccgtgggaga tcgatcccgg gcagccgcgg gtcgaggcgg cgccgctgcg
     6601 ctcgcgcctg cgccattaca ccaacctgtc agcgatgcag cccaagctgt tgaagctgct
     6661 gaaggcgcac acatggggcc ggaccgatca tgtggtcgcg gccgagcggg agcggctcgc
     6721 atgaacgcct tttcacccgt cgccggcgtg agcatccgga cggtcgagct ggcgcccaac
     6781 cgggaccgca tcgacgccta tgtcgtcgcc caggccggcg cgcagccctt ccaccgcccg
     6841 caatggagcg aggcggtcga gcgcggctgc ggccagcgcg cttattatct ggtggccgaa
     6901 cggccctccc gggcgctcgc cggggtgctg ccgctgaccg agatccgctc gtccctgttc
     6961 ggcaccgctc tggtctcggc cggcttcgcg gttggcggcg gcccgctggc cgaggacgac
     7021 accgtggccg aggcgctcgc caaggccgcc tggaacctga cgcgccagct cggctgcccg
     7081 tcgatcgaaa tgcgcggcgg ccgcgtgccg ctgggctgga agcgctgcga aggcgtctat
     7141 gcgggcttcg tgcgcgcgct ccccgccggc gacgaggcga tcctcaccgc gatcccgcgc
     7201 aagcagcggg cggaagttcg ccgcgcgcaa ggctttggcc tcgatgtctc gcacggctcc
     7261 tccgcttcgg accgcgacgc tcactattcg gtctatgccg agagcgtccg caatctcggc
     7321 acgccggtgt tcccccgcga actgttcggc gcgatgctcg acgcgttcgg cgaggatgcc
     7381 gacatcgtca ccgtctggaa ggacggagtg ccggtctcga gcgtcttgag tttctatctc
     7441 aacggcaccg tctatcccta ttggggcggc ggcacccgcg ccgcccggac gctgcgcgcc
     7501 aacgagctcg cttattacga gctgatgcgc cgcgccgccg cgcgcggctg cgaccgcttc
     7561 gacttcggcc gctccaagct cggcaccggc gcttttgcgt tcaagaagaa ttggggcttc
     7621 gagccgcagc ctttggtcta ttccatgcgt accgcccccg gcatgacgcc gcgcgagatc
     7681 aacccgctca gccccaaata ccggctcaag gtcgcggcct ggcagaggct gcccttgtgg
     7741 atcgccaacc ggctcggccc ccggatcgcg cgcgggctcg ggtgatgacc gacatcctgt
     7801 tcctcgccca ccgcatcccg tatccgcccg accggggcga caagatccgg tcgtggaaca
     7861 tgctgaagtt cctggccggc ctgggccggg tccacctcgc ctgcttcgcg gacgatccaa
     7921 aggatgcggc gcatctcgcg ggtctgaaag cggaactcgg gagcgcggtc ggccgcaccc
     7981 atgtcgagct gcgctcgacc ggccgcatcg ccgcggcctt gcaggcgctt cgcaccggcg
     8041 gcccggtgtc gctgccgctt ttcggcagtc agcgcatgca ggggttcgtc gacgaaattc
     8101 tcgccagcga gccggtcggc acgatcttcg ctttctccgg ccagatggcg cagttcgttc
     8161 cggcgggcgc gccgcagcgt ttcgtgatgg acttcgtcga catggattcg gccaagttcg
     8221 ccgattacgc ccgcgaaggc ggcggtcctt cggcttggat ccacgcccgc gaggccggca
     8281 aattgttcgc cttcgagcgc gccaccgccg cccgcgccga tctcagcctg ttcgtcagcg
     8341 aagccgaggc cgggctgttc cgcgccgagg cccggctgcc cggggccgac atccgggcga
     8401 tccagaacgg cgtcgacctc ggttattacg atcccgccgc tgacttcccg cggctcgccc
     8461 ccttcgcggg gccactgatc gtcttcaccg gccagatgga ctatcggccc aatgtcgagg
     8521 cggtgagcgc ctttacggcc gagagctttc cggcgatccg ccgcgcccac ccggacgccg
     8581 tattcgcgat cgtcggccgc agtcccgccg actcggtgcg ccggctccag cagcggccgg
     8641 gcatcgtcgt caccggcgcg gttcccgacg tgcgcagctg gctcgccgcc gccgatgtcg
     8701 tggtggcgcc gctccggatc gcgcgcggca tccagaacaa ggtgctcgaa gcgatggcga
     8761 tggcgcggcc ggtagtcgcc tcgccgcccg ccttcgaagg catcgaggcc gagtccgggc
     8821 gcgatctcgt cgttgccgac acgccggccg ccgaggccga ggcggtgctc gccttgctcg
     8881 ccgaccgcga ccgcgccgcc gggctcggcc gggcggcccg gcttcgcatg gaggaagcct
     8941 atcgctggca ggcgaggctg gcgccgctgg ccgcgatcct ggcccagccg cggcggaagg
     9001 cggcggcatg acggcacaca ccccgatcgc agccaccgct acggcccccg ccgccgccat
     9061 ttccgggtgg cggctccacc tcaccggcct cgcggccgcc gccgtcgcca tgctggcgct
     9121 cttccaccgc gacgtcgtcg acatcgtcga tctgtggtgg aacagctcga cctataatca
     9181 ttgcctgctg atcctgccga tcgtcgcctg gctggtgacc cagcgcctgc ccgagcttcg
     9241 cgggttgacg cccgccgcct gggcgcccgg gctgctgctg gtcgcggccg gggcaggggg
     9301 ctggctgctg ggcgaagccg gcggcgtcgg gctcgcccgc catggcggca tcgtgctgat
     9361 gctgcagggc gcggtcatcg cgtgcctcgg caaggcggta tcgcgcggcc tcgccttccc
     9421 gatcttctac gccttgttcc tgatcccggc cggcgaggaa ttggtgccgc cgatgcagac
     9481 gctgaccgcc gacatgtgca tggcgctgct cgccctggtc ggcgtcccgg cccatatcga
     9541 gggcatcttc atcaccaccc cgagcggcta tttcgaagtc gccgaagcct gttcgggcgt
     9601 caagttcctg gtcgcgatga tcgcttacgg cgcgctggtc gcgaacgtct gcttcaagtc
     9661 ggcctggcgc cgcgccgcct tcatgctggc cgccatcgtc atcccgatcc tcgccaacgg
     9721 ggtgcgcgcc tggggcacca tctacatcgc cgaatatacc ggcaccgatt tcgccgccgg
     9781 cttcgatcat gtcgtctacg gctgggtgtt cttcgcgatc gtgatcgctt tgctgatggc
     9841 ggccggctgg cgcttcttcg accacaagcc caacgacccc tggttcgatc ccgccgcgct
     9901 acaggccgaa ggcgagccag ccggacccgc caacaggctg gtcatcgtcg ccctcgccgc
     9961 cgctttcacg gccgccttgc cgctcggctg gtccgcggcc gtcgc
//
LOCUS       DASVRK010000101        15208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_390904, whole genome shotgun sequence.
ACCESSION   DASVRK010000101 DASVRK010000000
VERSION     DASVRK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 15208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15208
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390904"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VF589_03340"
     CDS             <1..1363
                     /locus_tag="VF589_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8256641.1"
                     /translation="MGAPDVEAASRILQGEDGRDVDAILLDHWLSGPAGTQLIEQIRA
                     LRPDLPLLILTAQTSVAVAVEAMRAGASDFLVKPIAPDRLLAALNTATDRRKKSGELR
                     PLSEKISKPLAFDEIVGSAPGFRAALAIAAKAARARVSVLIEGESGSGKEVIAQAIHT
                     ASPRGKKPMVTVNCGAIPANLVESVLFGHEKGAFTGAFDRHIGRFEDADGSTLFLDEV
                     GELPLETQVKLLRALETGEIQRVGGRTVQTVDVRIIAATNRRLIEEVAKGNFREDLYY
                     RLNVVHVDIPPLRARQSDIPPLARHLLARIAEQPGMRYLSITDDALAVLMAYGWPGNV
                     RQLQNALFRAAVLCEGNALTAADFPHIAQESTLSKRSDDYHAKALNGASNSAALSNGS
                     GITLFSSDGHLRPLEEIEADVIRLAIGHYRGRMTEVARRLGIGRSTLYRKLGELGIGD
                     AAA"
     gene            1438..2094
                     /locus_tag="VF589_03345"
     CDS             1438..2094
                     /locus_tag="VF589_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256642.1"
                     /translation="MNRPILAAVLLLAACGQQPQAAPAAEPSNIAASDSATGGSGPQE
                     AAPPTPAAAPAAAPDRRTAGSGSIGPLRFRYDPARLVALDAPVSVPPDWTRQVPGIKL
                     VAADRAAVIGRAECLYGQSGEASRCNATQEAGLALAIVAEPFADLSAKLSPELRRGIS
                     LAGRDGVSWQIGAEGEGAEHILLPAGNRTLLIVRHYRESGNADEAAVREVLANLKIDG
                     "
     gene            2190..2942
                     /locus_tag="VF589_03350"
     CDS             2190..2942
                     /locus_tag="VF589_03350"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8256643.1"
                     /translation="MDFSDGNILITGAASGIGAAAATLLSERGAKKLILADRDEEKLA
                     DFAFSLPSERQLMIGDVADEHLWSQADLTGLTHAVVNAGIGAGQPIDETSFAEWRRVM
                     SVNLDGAFLTLQAAMRAMKRGGGSIVITASAAGLKAEPGIAAYGASKAAVIHLARIAA
                     KEGAAHGIRVNAIAPGGVETPIWGSVPFFADLVERTGDEAAAFRAMAAGATPLGRFAK
                     PSEIAEQIAFLLSDAAAFTTGACLVADGGYSL"
     gene            2923..3786
                     /locus_tag="VF589_03355"
     CDS             2923..3786
                     /locus_tag="VF589_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX8256644.1"
                     /translation="MAAIPYNSSPAFAGEGDHAQHGGGASGASRSYLCLLLLALTACD
                     AADPQPSANSFEPAAFSAKPACESRNFEGTPFTACRFDAARDELRLVLAGRDGAPLRS
                     LAALEAALGRDADRVRYAMNAGMYDEAGRPIGLYVEAGEERKSLNTRDGEGNFHLMPN
                     GVFAVDGKGRPLVTPSRQFAKDIRSPRWATQSGPMLVIDGKLHPKFDANGESQLIRNG
                     VGVCGPGVAWFALSEEGVSFGRFARFFRDGLGCPNALYLDGTVSSLWDPGAGRQDAYA
                     ELGPMLVVLRR"
     gene            3861..5843
                     /locus_tag="VF589_03360"
     CDS             3861..5843
                     /locus_tag="VF589_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8256645.1"
                     /translation="MNMGYRRALGTLMLALSLALPAGTPAAAAQTEIPKSFSPVTDAF
                     DHERREVMIPMRDGVKLHTVIVIPKAGPKPAPIILSRTPYGADKPTSQSPSPFAAMAL
                     PLSDEPLLREGYIRVYQDVRGRYGSEGDYVMTLPVRGPLNRRKVDQTTDTHDTIAWLV
                     KNVPGNNGRVGITGVSYGGWLTLMGMLDPHPALKAAVPMYPMVDGWIGDDFYHNGALR
                     QTMFEWIYQMGADKSSEHSPPFGYRDMYEAYLAGVSADGVARRYKADRLPTWRKIVDN
                     PEYNAFWRGQAVQDLLAAAGPKVPTLVVHGLFDQEDNFGGIAAYEALEAKDTNNDMVH
                     LAVGPWNHGQSQGEGSSLGAVKWDSDTSLWFREKILLPFWNRHLKGDAAAPATPPVIA
                     FETGANEWRTYRSWPADGQVARTRLHLQPGGRLGFPAPAAAAPGYSEYVSDPAKPVPY
                     RVRPVLAMYNTESSWRNWLADDQRPFADRTDVLTFVSEPLTEPLTISGEVAAHLFAST
                     TGTDADWVVKLIDVYPPEVKANPQLGGYQLMVSGDIMRGRYREDPSRPKAIAPGQVAR
                     YRVRLPHANHTFKPGHRLMVQVKSSWFPLYDRNPQTYVANIAKARAADYRPATHRIHF
                     APHAASFIELPVVRAAGGAAAARSGSGGSPAPGARK"
     gene            5840..6265
                     /locus_tag="VF589_03365"
     CDS             5840..6265
                     /locus_tag="VF589_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256646.1"
                     /translation="MTGIALAASLAAAVQMAPLTLEQQSIDRVKPRLEAAVRLIDAAG
                     RADQAGFAALVLDGATIFSEAESRLAPLTMAALAPLAGRCKPAPSFRLGFDDEVTVHW
                     TCPGEPVRKLQTVFKFRAGRVAWASTEPSITYVLPPPPR"
     gene            complement(6394..7476)
                     /gene="folP"
                     /locus_tag="VF589_03370"
     CDS             complement(6394..7476)
                     /gene="folP"
                     /locus_tag="VF589_03370"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX8256647.1"
                     /translation="MSHRIYLRPTAFIDAPFGHDGKVARLAGGMQFFAAVELIGPAGA
                     ELVPVAEMEARLAGLGDRVGAAWRNLTSARAPLAVGGRTIRLDQPQVVGIVNVTPDSF
                     SDGGRHAEPAAAAEAGFAMAAAGAAIIDVGGESTRPGAKPVWEGDEIARVLPVVRTLA
                     GSGTAVSIDTRKAAVMEAALGAGAGLVNDVSALTWDERAAGVVARAGCPVVLMHHQGP
                     PETMQQAPRYERPVLLEVYDWLEARIAAAEAAGIARARILVDPGIGFGKTVTHNLELL
                     NGLAMLHGLGCAVMLGASRKRMIGALAGEAPAERRLAGSLALALKAAEQGAQLLRVHD
                     VEETVQALKVWRGMRDAALSPLPEGR"
     gene            complement(7741..8373)
                     /locus_tag="VF589_03375"
     CDS             complement(7741..8373)
                     /locus_tag="VF589_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256648.1"
                     /translation="MIMTDDHKHEEQTISFDFKTVSARKVAHREGQDEHLSPEERRCR
                     RIEQAVNEELWRPWENLMPKCEGEYDWRFCFPLRMPEIAPLIGVVWGDSACDCIEGDD
                     AEIVQIIVCNRYSNLVMAHFTINRVEVVRADGSPVPLLPDGSPSIQVVPLGPYCYGDI
                     PPCSCVVRQFMLRLRGAPGGKYRILLQGVCFEACFHQLREDCFTFEVCKD"
     gene            8757..9029
                     /locus_tag="VF589_03380"
     CDS             8757..9029
                     /locus_tag="VF589_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256649.1"
                     /translation="MFDRTYRSAMRNGSRLLFAIALLLFVSELLRGLQLFLPLGPDNA
                     VEPLGMTFVGHVLNALSLCAWPLLGSLLIDRLDRRTTPHASDQDPA"
     gene            complement(9146..10276)
                     /locus_tag="VF589_03385"
     CDS             complement(9146..10276)
                     /locus_tag="VF589_03385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX8256650.1"
                     /translation="MSVMGRVGTRARRPKAGPAADLPLERIIESDCIRAMAALPDDSI
                     DMVFADPPYNLQLGGDLFRPEGGRVDAVDDDWDKFDTFEAYDSFTRAWLAEARRILKP
                     DGTLWVIGSYHNIFRVGAALQDAGYWILNDIIWRKSNPMPNFRGTRFTNAHETLIWAS
                     KSEDSRYTFNYRAMKALNDELQMRSDWVLPICSGGERVKTDGGIKAHPTQKPESLLYR
                     VLLACTNPGDVVLDPFFGTGTTGAVARRLGRRWIGIEREAKYVKVARQRIASTLALDE
                     SAMRTMATKRAGPKVPFGALVETGMIEPGAILTDAKRRWQARVGADASVELAGRQGSI
                     HQIGAAAQGAPSCNGWTFWHVAEGEALVPLDALRQKYILTLG"
     gene            complement(10476..11108)
                     /locus_tag="VF589_03390"
     CDS             complement(10476..11108)
                     /locus_tag="VF589_03390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX8256651.1"
                     /translation="MAPCFKIEKAFPEPLAGVDEAGCAPLAGPVVAAAVVLDRVRFPR
                     GIDDSKALPAKTREAICARLHQSARVGIGIASVEEIDTLNIYWARMLAMSRAVEALGF
                     DPAWVLVDGNACPRWQRPSKAIVAGDAKCRSIAAASIVAKVTRDRIMADHARDHPGYG
                     WETNKGYPTPEHYRGLAALGVTPLHRRSFAPVRQMVLDLAPAPVPAQPVA"
     gene            complement(11196..12005)
                     /locus_tag="VF589_03395"
     CDS             complement(11196..12005)
                     /locus_tag="VF589_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEX8256652.1"
                     /translation="MHLPGPLWLVGCGNMAGAMLQGWLAAGLDPAAVTVIRPSGRQPA
                     PGIRTVTELPPGETPALVLLGVKPQKLTEVAPILAPALGPATILVSILAGVEQATLRA
                     LFQAPRTVVKAMPNLPVSLGKGVIELHSDSADEEARASVDRLMSALGHVEWFDDEGLF
                     NVASALTAAAPAFLFRFLDALAAGGTELGLAPEQAARLAAAMAEGASALAVAADASPA
                     ELARRVASPGGTTQAGLEVLDGQDGLRQLMLRALEASRRRGAEMAAAARGG"
     gene            complement(12102..12611)
                     /locus_tag="VF589_03400"
     CDS             complement(12102..12611)
                     /locus_tag="VF589_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8256653.1"
                     /translation="MNLDEYEIERETSAPIDMLEHYFSAHGWAYERSGDEEIVANFQG
                     SWAQYELRAIWREDDNVLQFLALPDIRVASDKRAATYETIGLINEQLWLGHFELWTTS
                     GLVLFRHAALLEGEEGGTMTLQQAETLVEAAIEECERFYPVFQFVLWADKTPQEAIAA
                     ALIETQGEA"
     gene            complement(12709..13038)
                     /locus_tag="VF589_03405"
     CDS             complement(12709..13038)
                     /locus_tag="VF589_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX8256654.1"
                     /translation="MQSESRFFDDIAKVLNGAAGTFAGMTREAEAGFRERMREWVGGL
                     DMVSRDEFEAVKAIAVAAREENMALKSRIDALEAAGAATGAAAAAPASASARGRKKPQ
                     DEAGRGD"
     gene            complement(13061..13615)
                     /locus_tag="VF589_03410"
     CDS             complement(13061..13615)
                     /locus_tag="VF589_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX8256655.1"
                     /translation="MTGIASKSQLRMSFLRYALVTVPLILLLGIVSGRVSDSGYGNAW
                     FDALVKPDIMPPGWLFAVVWPILYIMLGLSLAMILHARGARGRKLALGLFLAQLLLNF
                     AWSPVFFASHRVDDAFVIILVMLALSVVTAFLFARIRKGAALLMVPYLAWLAFAATLN
                     YRIAELNPNAETLVPQAGSADILL"
     gene            complement(13894..14580)
                     /locus_tag="VF589_03415"
     CDS             complement(13894..14580)
                     /locus_tag="VF589_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX8256656.1"
                     /translation="MTSIVKSIDRGSGNPVWDLPLRIFHWSIVALVGLSWWSAEQSFD
                     RVHFWSGYTLLFLLIFRLLWGLFGSSTARFTSFVRGPSAVFAYLRDGRHDRAGHTPLG
                     ALSVVGMLLALLVQVSSGLIQIDDDDFVEGPLSGLVDYDTALLAHDVHEVSFNILRGL
                     IALHLLAIAYYQFLRRKPLVRPMITGQARLPDGMSPMTPASKGRLVACVSAALAVTAG
                     ILLGAPAIGS"
     gene            complement(14555..15103)
                     /locus_tag="VF589_03420"
     CDS             complement(14555..15103)
                     /locus_tag="VF589_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8256657.1"
                     /translation="MRKLIAGMAVMLLVGCGPAADESEVLAEANSSGNAAGAAVEKAV
                     QQSAATPLQKEQALALMEERHENYEKIGDAMKGISRELKGDKPNLGEVRSGAATIAQL
                     APQVPSWFPAGTGPDVGKTEARAEIWQKPEDFAAKAQAFREAALAFNTAAQGSDLGSI
                     RSAHANLGKSCKGCHDLYREEH"
BASE COUNT         2409 a         5580 c         4901 g         2318 t
ORIGIN      
        1 gatgggcgcg cccgacgtcg aggcggcctc gcgcatcctc cagggcgagg acggccgcga
       61 cgtcgatgcg atcctgctcg atcattggct gtccggcccg gccggcaccc agctgattga
      121 gcagatccgc gccctccggc ccgatctccc cttgctcatc ctcaccgccc agacctcggt
      181 cgcggtcgcg gtcgaggcga tgcgcgccgg cgccagcgat ttcttggtga agccgatcgc
      241 gcccgaccgg ctgctcgcgg ccttgaacac cgccaccgac cgccgcaaga aaagcggcga
      301 gctgcgcccg ctctccgaaa agatctccaa gccgctggcg ttcgacgaga tcgtcggctc
      361 cgcgcccggc ttccgcgccg ccctcgccat cgccgccaag gccgcccgcg cccgcgtctc
      421 ggtcctcatc gaaggcgaaa gcggctcggg caaggaagtc atcgcccagg cgatccacac
      481 cgcctcgccg cgcggcaaga agccgatggt gacggtcaat tgcggcgcca ttcccgccaa
      541 cctcgtcgaa tcggtcctgt tcggccatga gaagggcgcc ttcaccggcg ccttcgaccg
      601 ccatatcggc cggttcgagg atgccgacgg ctcgaccctg ttcctcgacg aagtcgggga
      661 attgccgctc gagacgcagg tcaaattgct gcgcgcgctc gagaccggcg agatccagcg
      721 cgtcggcggc cgcaccgtcc agaccgtcga cgtgcgcatc atcgccgcca ccaaccgccg
      781 cctgatcgag gaagtcgcca agggcaattt ccgcgaggac ctctattacc ggctgaacgt
      841 cgtccacgtc gacatcccgc cgctccgcgc ccggcagagc gacatcccgc cgctcgcccg
      901 ccacctgctc gcccgcatcg ccgagcagcc cggcatgcgc tatctctcga tcaccgacga
      961 cgcgctcgcc gtgctgatgg cctatggttg gcccggcaac gtccgccagc tccagaacgc
     1021 tttgttccgc gccgccgtgc tgtgcgaggg caatgcgctg accgccgccg atttcccgca
     1081 catcgcgcag gaatcgacgc tcagcaaacg ctccgacgat tatcatgcga aggccttgaa
     1141 cggcgcctcc aacagcgccg ctctgtccaa cggctcgggc atcaccctat tctcgtccga
     1201 cggccatctg cgcccgctcg aggagatcga ggccgacgtc atccggctcg cgatcggcca
     1261 ttatcgcggg cgcatgaccg aagtcgcccg ccgcctcggc atcggtcgct cgaccctcta
     1321 tcgaaagctc ggcgaactcg gcatcggcga cgccgccgcc tgaagcgggc ggggcccgcg
     1381 cccgtccaaa caagttcagg gtgacgactg cggccgcacc agcgtagact tgggcgcatg
     1441 aaccgcccga tcctcgccgc cgtcctcctc ctcgccgcct gcggacagca gccgcaagcc
     1501 gcccccgccg ccgagccgtc gaacatcgcc gccagcgatt cggccacggg cggcagcggt
     1561 ccccaggaag ccgccccgcc cacacccgcc gccgccccgg cggccgcgcc cgaccgccgc
     1621 accgccggca gcggctcgat cggcccgctc cgcttccgct acgatccagc ccggctggtc
     1681 gcgctcgacg ctccggtctc ggtgccgccc gactggaccc gacaggttcc cggcatcaag
     1741 ctggtcgccg ccgaccgcgc cgctgtgatc ggccgggccg aatgcctgta cggccagtcg
     1801 ggtgaagcca gccgctgcaa cgccacccag gaagccggcc tcgcccttgc catcgtcgcc
     1861 gagcccttcg ccgatctcag cgcgaaattg tcgcccgagc ttcgccgcgg catcagcctc
     1921 gccggccgcg acggcgtctc ctggcagatc ggcgccgaag gcgaaggcgc cgagcacatc
     1981 ctgctccccg ccggcaaccg caccctgctg atcgtccgcc actatcgcga aagcggcaat
     2041 gccgatgagg ccgcggtccg cgaggtgctg gcgaacctga agatcgacgg ctgaggcctg
     2101 cggcagaacg cggggaccat cccgcaagcc ccccccccca ggccgccggc acatggcgca
     2161 acccattcgc ccctgctaga ctcgcgccca tggacttcag cgacggcaac atcctcatca
     2221 ccggcgccgc ctccggcatt ggcgccgccg ccgccaccct gctttccgaa cgcggcgcca
     2281 agaagctgat cctcgccgac cgcgacgagg aaaagctcgc cgacttcgct ttctcgctgc
     2341 cgagcgagcg gcaattgatg atcggcgacg tcgccgacga gcatctgtgg agccaggccg
     2401 acctcaccgg cctcacccat gccgtcgtca atgccggcat cggcgccggc cagccgatcg
     2461 acgagaccag cttcgccgaa tggcggcggg tgatgtcggt caatctcgac ggcgccttcc
     2521 tcaccttgca ggcggcgatg cgggcgatga agcgcggcgg cggatcgatc gtcatcaccg
     2581 cctcggccgc gggcctcaag gccgagcccg gcatcgccgc ttacggcgcg tccaaggcgg
     2641 cggtgatcca cctcgcccgg atcgcggcca aggagggcgc cgcccacggc atccgcgtca
     2701 acgccatcgc gcccggcggc gtcgagactc cgatctgggg cagcgtcccc ttcttcgccg
     2761 acctggtcga gcgcaccggc gacgaagccg ccgccttccg cgccatggcc gccggcgcga
     2821 ctcccctggg ccgcttcgcc aaaccctcgg agatagcgga gcaaatcgcc ttcctcctct
     2881 ccgacgccgc cgccttcacc accggcgcct gcctcgtcgc cgatggcggc tattccctat
     2941 aattcctccc ctgcttttgc aggggagggg gaccatgcgc agcatggtgg aggggcaagc
     3001 ggcgccagcc gctcctacct atgccttctc ctcctcgccc taacggcctg cgacgccgcc
     3061 gacccgcagc catcggccaa cagcttcgaa cccgcggcct tcagtgccaa acccgcctgc
     3121 gaaagccgca acttcgaagg cacccccttc accgcctgcc gcttcgacgc cgcccgcgac
     3181 gaactgcgtc tcgtcctcgc cggccgcgac ggagcaccgc tccgaagcct cgccgcgctc
     3241 gaagccgcgc tcggccgcga cgccgaccgc gtccgttacg ccatgaacgc cggcatgtat
     3301 gacgaggccg gccgcccgat cggcctctat gtcgaagccg gcgaggagcg caaaagcctc
     3361 aacacgcgcg acggcgaagg caatttccac ctgatgccga acggcgtctt cgcggtggac
     3421 ggcaagggcc gcccgttggt caccccaagc cgccagtttg ccaaggacat ccgctccccg
     3481 cgctgggcca cccagtccgg cccgatgctg gtcatcgacg gcaagctcca cccgaagttc
     3541 gacgcgaatg gcgaatcgca gctgatccgc aacggcgtcg gcgtctgcgg ccctggcgtc
     3601 gcctggttcg cgctcagcga ggaaggcgtc tcgttcggcc gcttcgcccg cttcttccgc
     3661 gacggcctcg gctgccccaa cgcgctctac ctcgacggca ccgtctccag cctgtgggat
     3721 cccggcgccg gccgccagga tgcctatgcc gagctcggcc cgatgctcgt cgtgctccgc
     3781 cgctgagatc ccccctcccc ccgtcgcgcg ccatattgcc agccccgccg cgccgcctta
     3841 gcctcggcgc aaaaggggac atgaacatgg gatatcgccg cgcgctcgga accctgatgc
     3901 tggcgctgtc gctggcgctt cccgccggca ccccggcggc ggccgctcag accgagatcc
     3961 ccaagagctt ctcgccggtc accgacgcct tcgaccatga gcggcgcgag gtgatgatcc
     4021 cgatgcgcga cggcgtgaag ctccacaccg tcatcgtcat ccccaaggcg gggcccaagc
     4081 ccgcgccgat catcctgtcg cgcaccccct acggcgccga caagccgacc agccagtcgc
     4141 cgagcccctt cgccgccatg gcgctgccgc tttccgacga gccgctgctg cgcgaaggct
     4201 atatccgcgt ctaccaggac gtgcgcggcc gctacggcag cgaaggcgat tatgtgatga
     4261 ccctgccggt gcgcgggccc ctcaaccgcc gcaaggtcga ccagaccacc gacacccacg
     4321 acaccatcgc ctggctggtg aagaacgtgc ccggcaataa cggccgcgtc ggcatcaccg
     4381 gcgtctctta tggcggctgg ctgaccctga tgggcatgct cgacccgcat cccgcgctca
     4441 aggcggcggt gccgatgtac ccgatggtag atggctggat cggcgacgat ttctaccaca
     4501 atggcgcgct tcgccagacc atgttcgagt ggatctacca gatgggcgcg gacaagagca
     4561 gcgagcattc gccgcccttc ggctatcgcg acatgtacga ggcctatctc gccggcgtct
     4621 cggccgacgg cgtcgcccgc cgctacaagg ccgatcgcct gccgacctgg cgcaagatcg
     4681 tcgacaatcc cgaatataac gctttctggc gcggccaggc ggtccaggac ctgctcgccg
     4741 ccgccgggcc gaaggtcccg accctcgtcg tccacggcct gttcgaccag gaggacaatt
     4801 tcggcggcat cgccgcctac gaggcgctcg aggccaagga tacgaacaat gacatggtcc
     4861 acctcgccgt cggcccgtgg aaccacggcc agtcgcaggg cgagggctcg tcgctcggcg
     4921 cggtcaaatg ggattccgac accagcttgt ggttccgcga gaagatcctg ctcccattct
     4981 ggaaccgcca tctgaagggc gacgccgccg ctcccgccac gccgccggtg atcgcgttcg
     5041 agacgggtgc caacgaatgg cgcacctatc gcagctggcc ggccgacggc caggtcgccc
     5101 gcacccggct ccacctccag cccggcggcc gcttgggctt ccccgccccc gccgccgccg
     5161 cgcccggcta ttccgaatat gtctccgatc cggccaagcc ggttccctat cgggtgcggc
     5221 cggtcctcgc catgtacaac accgaatcga gctggcggaa ctggctcgcc gacgaccagc
     5281 gcccgttcgc cgaccgtacc gacgtgctca ccttcgtctc cgagccgttg accgagccgc
     5341 tcaccatctc cggcgaggtc gccgcccatc tcttcgcctc caccaccggc accgacgccg
     5401 actgggtggt gaagctgatc gacgtctacc cgcccgaagt gaaggccaat ccgcagcttg
     5461 gcggctacca gctgatggtt tccggcgaca tcatgcgcgg ccgctaccgc gaggacccgt
     5521 ccaggcccaa ggcgatcgcc ccgggccagg tcgcccgcta ccgcgtccgc ctcccgcacg
     5581 ccaaccacac cttcaagccc ggccaccggc tgatggtgca ggtcaagtcg agctggttcc
     5641 cgctctacga ccgcaacccg cagacctacg tcgccaacat cgccaaggcc cgcgccgccg
     5701 actaccgccc cgccacccac cgcatccact tcgccccgca tgccgccagc ttcatcgaac
     5761 tgccggtggt gcgggccgct ggtggcgcgg cggcagcgcg aagtgggagc ggcggctcgc
     5821 ccgcgccggg agcccggaaa tgaccggcat cgcgctcgcc gcctcgctcg ccgcggccgt
     5881 tcaaatggcg ccgctcacgc tcgagcaaca atcgatcgat cgggtgaagc cgcggcttga
     5941 agcggcggtg cgcctgatcg acgccgccgg gagggccgac caggccggct tcgccgcttt
     6001 ggtgctggac ggcgcgacca tcttttcgga ggccgaaagc cggctcgcgc cgctgacgat
     6061 ggcggcgctg gcgccgctcg ccgggcgctg caagccggcg ccgagcttcc gactgggctt
     6121 cgatgacgag gtcaccgtcc attggacctg cccgggcgag ccggtccgga agctgcagac
     6181 ggttttcaag ttccgcgccg ggcgcgtcgc ctgggcctcg accgagcctt cgatcaccta
     6241 cgtcctccct cccccgcctc gctgaccggc gtggcggcgg aggcccgccg ttggtagagc
     6301 ggggcgcacc gattccgggc gcctcctccg ggggctaact caccccgacc gatgaggccg
     6361 ctgcatcatg cccgcctgat ccttggtgcg gcctcatctg ccctcaggga ggggagacaa
     6421 agccgcgtcc cgcatccccc gccacacctt cagcgcctgc accgtctcct cgacgtcgtg
     6481 cacgcgcagc aattgcgccc cctgctcggc cgccttcagc gccagcgcca gcgagcccgc
     6541 cagccgccgc tcggccggcg cctcgcccgc cagcgcgccg atcatgcgct tgcggctggc
     6601 acccagcatg accgcgcagc cgagcccgtg cagcatcgcc agcccgttca ggagctcgag
     6661 attgtgcgtc acagtcttgc cgaagccgat cccgggatcg accaaaatcc gcgcccgcgc
     6721 gatccccgcc gcctcggccg ccgcgatccg cgcctcgagc cagtcataga cctcgagcag
     6781 caccggccgc tcgtaacgcg gcgcctgctg catcgtctcg ggcggacctt gatggtgcat
     6841 gaggacgacc gggcagcccg ccctggccac cacccccgcc gcgcgctcgt cccaggtcag
     6901 cgctgacacg tcgttgacca gccccgcccc cgcgccgagc gccgcctcca tcaccgcggc
     6961 cttgcgcgta tcgatcgaca ccgccgtccc cgatccggcg agcgtgcgca ccaccggcag
     7021 cacccgcgcg atctcgtcgc cttcccagac cggcttcgcc cccggccgcg tcgattcgcc
     7081 gccgacgtcg atgatcgccg cccccgccgc cgccatggcg aagcccgcct cggccgccgc
     7141 cgccggctcc gcatgccgcc cgccgtccga gaagctgtcc ggcgtcacgt tgacgatgcc
     7201 caccacctgc ggctggtcga gccggatcgt ccgcccgccg accgcaagcg gcgcccgcgc
     7261 gctggtcagg ttgcgccacg ccgcccccac ccggtcgccg agccccgcca gccgcgcctc
     7321 catttccgcc accggcacca gctccgcccc cgccggcccg atcaactcga cggcggcgaa
     7381 gaactgcatc cccccggcca gccgcgcgac cttcccgtca tgcccgaacg gcgcgtcgat
     7441 gaaagcggtg gggcggaggt agatgcggtg gctcatccgc ccgccttatc gcgggggcgg
     7501 tcgcgtagga accgggttat cggggggcgg cactctgccc atggcccgca tacccctggg
     7561 gagttgcgtc attccagcga aagctggaat ctccttcttc tcccgctggt gtcgatggcc
     7621 ggcgaggacc tccggtaccg cgacgctgtg gcctcggtgc cctcggcaaa ccccgccggc
     7681 gcgactgagc cgcacaggtt cgacaaatcc gtccgccgct gcccacgccg gcgcccggcc
     7741 tcagtccttg cagacctcga acgtgaagca gtcttcccgc aattggtgga agcatgcctc
     7801 gaagcagacg ccctggagga gaatgcggta tttcccgccc ggcgcgccgc gcagccgcag
     7861 catgaactgg cgcacgacgc agctgcacgg cgggatgtcg ccgtagcaat aggggccgag
     7921 cgggacaacc tggatcgacg ggctgccgtc cgggaggagc ggcaccggcg atccgtccgc
     7981 ccgcacgacc tcgacccggt tgatcgtgaa gtgcgccatg acgaggttgg aatagcggtt
     8041 gcaaacgatg atctggacga tctcggcatc gtctccctcg atgcagtcgc acgcgctgtc
     8101 gccccagacc acgccgatca gcggcgcgat ttccggcatg cggagcggaa agcagaacct
     8161 ccagtcatac tcgccctcgc acttgggcat caaattctcc cacgggcgcc acaattcctc
     8221 gttgaccgcc tgctcgatcc gtctgcagcg cctttcctcc ggcgacaggt gctcgtcctg
     8281 cccttcacga tgtgcgactt tcctggcgct caccgtcttg aaatcaaagc tgatcgtctg
     8341 ctcttcatgc ttgtggtcgt cggtcatgat catgcccttt ccagcagatt gcgtgcgaac
     8401 gcacgcgcaa gacttcctcc ggcgagcgcc ggcttgaaca tcatgctacg cataatcgtg
     8461 actataggaa ggggttgatg atgctctctt cgaagtacaa gtcaagcaat gcatctattc
     8521 tcaagctagt cttgacataa aagctgatcc attttggttc caacggcgtg aatggccgga
     8581 aataagggcc ggagcgtcgg cttcccccgc cgcgagcttc cagcttgcgc tggaatgacg
     8641 aagggtgcgc tcaccatcct cggcgggggc cgtcattcca ccgcaagccg aatcccccct
     8701 cccccgcctt ccaatcaccg ccccgcctgc tatgcctgcc gccaggaggc acgcggatgt
     8761 tcgaccgaac ctaccgatcg gcaatgcgca acggatcgcg cctgttgttc gcgatcgcgc
     8821 tcctcctctt cgtctcggaa ctgcttcgag gcttgcagct cttcctgccg ctcggccccg
     8881 ataatgcggt cgagccgctc ggcatgacct tcgtcgggca cgttctgaac gccctttcgc
     8941 tgtgcgcctg gccgctcctc ggctcgctgc tcatcgaccg cctcgaccgg cggactacgc
     9001 cgcacgcatc ggatcaagat ccggcctgaa ctcgcgccaa caaaccgcgc cgcgccggcc
     9061 ccacatggct gccatttcca tcatcgaagg ccggcgcgcc cgtttgcgag gcactttcgc
     9121 caacatcggg ccgatcggcc gcggatcagc cgagggtgag gatgtatttc tgccgcagcg
     9181 cgtcgagcgg gaccagcgcc tcgccctcgg cgacgtgcca gaaggtccag ccgttgcagg
     9241 agggcgcgcc ctgggcggcg gcgccgatct ggtggatcga gccctgccgg ccggccagct
     9301 cgaccgaggc gtcggccccg actcgcgcct gccagcggcg cttggcgtcg gtgaggatcg
     9361 cgcccggctc gatcatgccg gtctcgacca gcgccccgaa cggcactttc ggcccggccc
     9421 gcttggtcgc catggtccgc atcgcgctct cgtcgagcgc gagcgtggaa gcgatacgct
     9481 ggcgcgcgac cttcacatat ttggcctcgc gctcgatgcc gatccagcgc cgcccgagcc
     9541 ggcgggcgac cgcgccggtg gtgccggtgc cgaagaacgg gtcgagcacg acgtcgcccg
     9601 gattggtgca ggcgagcagc acccggtaga gcaggctttc cggcttctgg gtcggatgcg
     9661 ccttgatgcc gccgtcggtc ttgacccgct cgccgcccga gcagatcggc agcacccagt
     9721 cggagcgcat ctgaagttcg tcgttcaatg ccttcatcgc gcggtagttg aaggtgtagc
     9781 ggctgtcctc gctcttcgac gcccagatga gcgtctcatg ggcgttggtg aagcgggtgc
     9841 cgcgaaagtt cggcatcggg ttcgacttgc gccagatgat gtcgttgagg atccagtagc
     9901 cggcgtcctg cagcgcggcg ccgacccgaa agatattgtg gtagctgccg atcacccaca
     9961 aggtgccgtc gggcttcagg atgcggcgcg cctcggccag ccaggcgcgg gtgaagctgt
    10021 cataggcttc gaacgtatcg aacttgtccc aatcgtcgtc gaccgcgtcg acgcggccgc
    10081 cctcgggccg gaacaggtct cctccgagct ggaggttata gggcgggtcg gcgaacacca
    10141 tgtcgatgct gtcgtcgggc aaggcggcca tcgcgcggat gcagtccgat tcgatgatcc
    10201 gttccagcgg cagatcggcc gcgggccccg cctttgggcg ccgcgcgcgc gttcccaccc
    10261 gtcccatgac actcattcag ccttgccccc ggcagcccct gtcgccgcca tgctgagtca
    10321 gccggactcc gcggtcaagc ggaacagttc aaacgcgctg ttaaccatgg ttaaaatgtt
    10381 acaggaacga aaggagtccc cgctagcgct tgtggcggcg ggcgaagctg agactcaata
    10441 tgaagtggtg tggccgccag gactcacagg atgtgctatg cgacgggctg agcgggcacg
    10501 ggagcgggcg cgagatcgag caccatctgg cgcaccggcg cgaagctgcg gcggtggagc
    10561 ggggtgacgc cgagcgcggc caggccgcga taatgttcgg gcgtgggata gcccttgttg
    10621 gtttcccagc catagccggg atgatcgcgc gcatggtcgg ccatgatccg gtcgcgggtg
    10681 accttggcga cgatggacgc ggcggcgatc gagcggcatt tggcgtcgcc ggcgacgatc
    10741 gccttggacg gccgctgcca gcgcgggcag gcattgccgt cgaccagcac ccaggccggg
    10801 tcgaagccca gcgcctcgac cgcgcggctc atcgccagca tccgcgccca atagatgttg
    10861 agcgtgtcga tctcctcgac cgacgcgatg cctatgccga ccctggccga ctggtggagc
    10921 ctggcgcaga tcgcctcccg cgtcttggcg gggagcgcct tcgaatcgtc gatgccgcgc
    10981 gggaatcgca cccggtcgag cacgaccgcg gcggcgacca ccggccccgc cagcggcgcg
    11041 cagccggcct cgtcgacgcc ggctagcggc tcggggaatg ctttctcgat cttgaagcag
    11101 ggcgccatcg cccgagcgta ggccaagccg gcgccggctt gccagcgaaa gctgttggcg
    11161 gggcgcgctt ttcctgtgga tagcgcggcc gaccttcatc caccccgtgc cgcggcggcc
    11221 atttcggcgc cgcggcggcg cgaggcttcg agcgcgcgca gcatgagctg gcgcaggccg
    11281 tcctggccgt cgagcacttc gaggcccgcc tgggtggtgc cgcccgggct cgcgacccgc
    11341 cgcgccagct cggcggggct cgcatccgcg gcgacggcta gcgcgctcgc cccttcggcc
    11401 atggcggccg cgaggcgggc ggcctgctcc ggagcgaggc cgagctcggt cccgccggcg
    11461 gcgagcgcgt cgaggaagcg gaacaggaag gcgggcgcgg cggcagtgag cgcgctggcg
    11521 acgttgaaca ggccctcgtc gtcgaaccat tcgacatggc cgagcgccga catcagccga
    11581 tcgaccgagg cgcgcgcctc ttcgtccgcc gagtcgctgt gaagctcgat cacgcccttg
    11641 ccgaggctga ccggcaggtt cggcatcgcc ttcaccactg tgcgcggcgc ttggaacaag
    11701 gcgcgcaggg tcgcctgttc gacgccggcg aggatcgaaa ccaagatcgt cgccggaccg
    11761 agcgcgggcg cgaggatggg ggcgacttcg gtgagcttct gcggcttgac gccgagcagc
    11821 accagggccg gggtctcgcc cggcggcaat tcggtcacgg tgcggatgcc gggcgcgggc
    11881 tgccggccgc tggggcggat gacggtgacc gcggccggat ccagcccggc ggcgagccag
    11941 ccctggagca tggcgcccgc catgttgccg cagccgacca gccagagcgg gcctgggagg
    12001 tgcattgcgt ccactatctc tcccgcaaag aaggacagcc cttcgacagg ctcagggcga
    12061 acggagctgc tcccgccgcg cggatgtacc ccttccgcgc gctacgcctc gccctgcgtt
    12121 tcgatgagcg cggcggcgat ggcttcctgg ggggtcttgt cggcccacag gacgaactgg
    12181 aagaccgggt agaaacgctc gcattcctcg atcgcggctt cgaccagcgt ctcggcctgc
    12241 tgcagcgtca tcgtgccgcc ctcctcgcct tcgagcaagg cggcgtggcg gaacaggacc
    12301 aggccggagg tcgtccagag ctcgaaatgg ccgagccaga gctgctcgtt gatgaggccg
    12361 atcgtctcgt aagtggcggc gcgcttgtcg ctggcgacgc gaatgtcggg cagcgccagg
    12421 aactggagga cgttgtcgtc ctcgcgccag atcgcgcgaa gctcatattg cgcccagctg
    12481 ccctggaaat tggcgacgat ctcctcgtcg cccgaacgct catacgccca gccatgggcg
    12541 ctgaaataat gttcgagcat gtcgatgggg gcgctggtct cccgctcgat ctcatattcg
    12601 tccaagttca tcgcccaatc cccggcccca attcccaagt cgaatcctgc ccttgccccc
    12661 ggggagaggc aagtgcgggc ggacggtcgg aggcaacaaa gctgtggatt agtcgcccct
    12721 tccggcctcg tcctgcggct tcttgcggcc gcgggcggaa gccgacgccg gagcggccgc
    12781 ggcagcgccg gtcgcggccc cggccgcctc gagcgcgtcg atgcggctct tgagcgccat
    12841 attctcctcg cgcgcggcga ccgcgatcgc cttcaccgcc tcgaactcgt cgcggctcac
    12901 catgtcgagc ccgcccaccc actcgcgcat ccgctcgcga aaacccgcct cggcctcgcg
    12961 ggtcatgccg gcgaacgtgc cggcggcgcc gttcagcacc ttggcgatgt cgtcgaagaa
    13021 gcggctttcg gactgcatgg tttatgctcc tgagatcgga tcagagcagg atatcggcgc
    13081 tgcccgcctg cgggacaagg gtctcggcat tggggttcag ctcggcgatc cggtagttga
    13141 gcgtcgccgc gaaggcgagc caggcgagat aggggaccat cagcagggcc gcgcccttgc
    13201 ggatgcgggc gaacagaaag gcggtgacga ccgacagcgc cagcatgacg aggatgatga
    13261 cgaaggcgtc gtcgacgcgg tgggaggcga agaagaccgg cgaccaggcg aagttcagca
    13321 gcaattgcgc gaggaagaga ccgagcgcga gcttgcggcc gcgggcgccg cgggcgtgca
    13381 ggatcatggc gagcgatagg ccgagcatga tgtagaggat cggccagacg accgcgaaca
    13441 gccagccggg cggcatgatg tcgggcttca cgagagcgtc gaaccaggca ttgccatagc
    13501 cggaatcgga aactcggccg gagacgatgc cgagcagcag gatcagcggc acggtgacga
    13561 gcgcgtagcg caggaacgac atgcgcagct gcgacttgga tgcaatcccg gtcatcctgc
    13621 ctccgaacca gagccgggcg gcgcgcgcaa gccagaaata agttgaggaa gttgaggctt
    13681 gccgagagcc ggtccgccgg agtgcgccga aagtggacgg agtggacgga gtggacggct
    13741 ggatagggtt agcgcggggg ctacgcctgc aagctgtaac ggggtgcggt gtgggagagc
    13801 ggaaaggagc taggccgggc atgccgccga gcgtgacaga cgaaatccta ctttgggact
    13861 cccattcgtg cgcgaagcgg aggggccgtg cgcttaggag ccgatcgccg gggcacctag
    13921 caggatgccc gcagtgacgg ccagggccgc cgatacacag gcgacgagcc gacccttcga
    13981 cgccggtgtc atcggcgaca tcccgtccgg cagccgcgcc tgtccggtga tcatcgggcg
    14041 caccagcggt tttcgccgca ggaactggta gtaggcgatg gcgagcaggt gcagcgcgat
    14101 cagcccccgc aggatgttga agctgacctc atgcacgtcg tgcgcgagca gcgccgtgtc
    14161 gtagtcgacg agccccgaca gcggcccctc gacgaaatcg tcatcgtcga tctggatgag
    14221 cccgctcgag acctggacga gcagggccag cagcatgccg acgacgctga gcgcgccgag
    14281 gggcgtgtga cccgcccggt cgtgccggcc gtcccgcaga taggcaaaaa cggccgaggg
    14341 cccgcggacg aagcttgtaa agcgcgccgt cgaactgccg aacaagcccc acagcagccg
    14401 gaagatgagc aggaacagga gcgtgtaccc agaccagaaa tggacccggt cgaagctttg
    14461 ttcggccgac caccagctca atccgaccag agcgacgatc gaccagtgga atattcggag
    14521 ggggaggtcc cagacggggt tgcccgaacc tctatcaatg ctcttcacga tagaggtcat
    14581 ggcagccctt gcagctcttg ccgagattgg cgtgagccga gcggatcgag cccagatcgg
    14641 agccttgggc ggccgtattg aacgccaaag cggcttcgcg gaacgcctga gccttggccg
    14701 cgaaatcctc cggtttctgc cagatctcgg cgcgcgcctc ggtcttgccg acgtccggcc
    14761 cggtgccggc gggaaaccag ctcgggacct gcggggcgag ttgggcgata gtcgccgccc
    14821 cggaccgcac ctcgccgagg ttcggcttgt cgcccttgag ctcgcggctg atccccttca
    14881 tggcatcgcc gatcttctcg taattttcgt gacgctcttc catcagcgcc agcgcctgct
    14941 ccttctgcag cggcgtggcg gcgctttgct ggaccgcctt ctccacggcc gcgccggcgg
    15001 catttccgct ggaattcgcc tccgccagaa cctcgctctc gtccgcggcc gggccgcatc
    15061 cgacgagcag catcacggcc atgccggcga tcaactttct caacgcacct ctcctaattt
    15121 gacgcgtagg cccaagtgcc agatttcggc gccgcaggaa acatgtgggc tgacgccccc
    15181 tctacgtcac ccaaagcgaa aggaggcg
//
LOCUS       DASVRK010000102        43316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_267735, whole genome shotgun sequence.
ACCESSION   DASVRK010000102 DASVRK010000000
VERSION     DASVRK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 43316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43316
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267735"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..817
                     /locus_tag="VF589_03425"
     CDS             2..817
                     /locus_tag="VF589_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8256658.1"
                     /translation="MPGHESQLEKKPEWEPRYPGSGRLEGKVALITGADSGIGRAVAA
                     LFAREGADVAILYLCEHDDAAKTREIVEAEGRRAIAIAGDVGDKDFCERAVAETVAKL
                     GRLDILVNNAAEQHPDTEITDITEEQLKRTFQTNIFGYFFMVQAARPHLQEGAAIINC
                     TSVTMYKGSKELLDYSATKGAITAFTRSLSENLIDKGIRVNAVAPGPIWTPLNPSGGS
                     TPEKMEHFGEDVPMGRPGQPNEVAPSFLFLACEDSSYMSGQVLHPNGGEIVGS"
     gene            874..1287
                     /locus_tag="VF589_03430"
     CDS             874..1287
                     /locus_tag="VF589_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256659.1"
                     /translation="MLRIVSGAVAGLLAAFAAIMAIEYVGHAIYPLPADAALRDPEAP
                     VDALSMPLPVLLIVVFAWFAGALIGGAVAKRINGRWWSPWLVAGMVALGGILAVTMMP
                     HPTWMQIAAVAAPLLGGLFASHAVWGDRRATDGAL"
     gene            1274..1612
                     /locus_tag="VF589_03435"
     CDS             1274..1612
                     /locus_tag="VF589_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEX8256660.1"
                     /translation="MERYKSFREFWPFYLREHSKPRTRALHYAGTTLVVLIALGALVT
                     GTWWLLAAMPVAGYGFAWASHAAVERNRPATFTYPGWSLKADFLMWWLWLTGRLGPEL
                     EAAGVRPKAR"
     gene            complement(1609..2043)
                     /locus_tag="VF589_03440"
     CDS             complement(1609..2043)
                     /locus_tag="VF589_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX8256661.1"
                     /translation="MTPLFTIGYEGKTLPEFLGELSAAGIELLIDVRAVAASRRPGFS
                     KAALAGALKERGIDYLHLRPLGTPAAGREAARKGRTGEMRAIYAGQLETPEAELAFVK
                     AMSEARERRAALLCFEKDAPRCHRAVLAERMAAGEGFEISDL"
     gene            complement(2015..2260)
                     /locus_tag="VF589_03445"
     CDS             complement(2015..2260)
                     /locus_tag="VF589_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256662.1"
                     /translation="MASLSHRLFKAVSEGRRLRWRPESREQVLARLLVKRAEAQQAGL
                     DDLEASLRKQISWSLPMRGGGEDASDADHDPAVHDRL"
     gene            complement(2376..2696)
                     /locus_tag="VF589_03450"
     CDS             complement(2376..2696)
                     /locus_tag="VF589_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX8256663.1"
                     /translation="MNMAEPMLCPIDRTPLVMSERQNIEIDYCPTCRGVWLDRGELDK
                     IIERSAADAPAAAAPPPQPRSAPPPPPQMGHAPHPGYGGGYDTGHGQPYRKRKKSFLE
                     ELFD"
     gene            3291..3548
                     /locus_tag="VF589_03455"
     CDS             3291..3548
                     /locus_tag="VF589_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256664.1"
                     /translation="MRNLLTIAAGGAMLMLTACGGQGDDALGDNVADAAENRADGLED
                     AADNASGAQEDSLEAQAEITRDTGQAQEEAIDDADVVVNNQ"
     gene            complement(3550..4587)
                     /gene="dinB"
                     /locus_tag="VF589_03460"
     CDS             complement(3550..4587)
                     /gene="dinB"
                     /locus_tag="VF589_03460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX8256665.1"
                     /translation="MDAFYASVEQRDHPELRGKPLAVGGGVRGVVAAASYESRKWGVR
                     SAMPAVTARRLCPELIFVRPRFDAYRAVSQQIRAIFLDYTPHVEPLSLDEAYLDVTED
                     QKGIGIATDIAREIRARIKAETGLTASAGVSYNKFIAKLASDQNKPDGLCVVTPQQGP
                     AFVARLPVKRFHGIGPKTAEKMARLGIETGADLRAVSLEFLVHNFGSAGKYYHNLARG
                     ICHREVRADRERKSIGAEDTFSADLRDEADLLAELDRIGFHVWERIAERGIAGRTVTL
                     KVKYEDFRIVTRAQSLPAPVADGRSFVETGKALLRGLLPAEKGIRLLGLTLSGLGGGE
                     QAEPPQLRLPI"
     gene            complement(4659..5063)
                     /locus_tag="VF589_03465"
     CDS             complement(4659..5063)
                     /locus_tag="VF589_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256666.1"
                     /translation="MRERVALAALATTLVLAAPAEAQQPVDSRPLAAQRLGPPDQGPA
                     YLAPGAGLFPSQGSGFSVTTQNYSEQDGEPAIRKGLVGSVEVAPNATIGIGLFSVNRY
                     SRREPEFRRIQPMRDVRGQEKKIAAVGLSFSF"
     gene            5314..6468
                     /gene="crtY"
                     /locus_tag="VF589_03470"
     CDS             5314..6468
                     /gene="crtY"
                     /locus_tag="VF589_03470"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HEX8256667.1"
                     /translation="MVRGKRDGLLIAGGGLAGSLAAIAMAKARPEVPLLLVQEGESFG
                     GNRIWSFFDADITDGDRWLAEPLLSGSWPGYYVAFPGHSRKLKAGMSCVRSADVDRLV
                     RETLRPDQYRLGTRIVAVRENELVLLGGERIKADGALDARGAANLSMLDLAWQKFVGR
                     EYRLAAPHGLDRPVLMDATVEQSHGCRFVRCVPLGPDRMLIEDIYYSNSPELDTAALG
                     GRIEAYATKRGWPTAELEQEVSDVLPVALGGDFGAFWRVGGARVAKLGLRGGFFHPTT
                     GSGLADAVHNAVLLSQQRDLGGAALHDLFESQAAQLWKRREFYRTFNTMLFRGAAPHE
                     RAAMFEQLYRLDPALIARFHAGRPTVLDRMRVTGLKPPAPAKASTAAPRK"
     gene            complement(6476..6901)
                     /locus_tag="VF589_03475"
     CDS             complement(6476..6901)
                     /locus_tag="VF589_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256668.1"
                     /translation="MKLFDWISRVIMLVLASLVSLSIIGAIASIPSGSIPTTMIETAP
                     PAPQPDHGAEPAAGPSRPEAPPGPAAQPASPGAAVGTIAPPPPQEADGAEPWLESIFY
                     ALLALVGIAALATLLLWRSLQERRRLADAVETLAARLRA"
     gene            7044..8243
                     /gene="sucC"
                     /locus_tag="VF589_03480"
     CDS             7044..8243
                     /gene="sucC"
                     /locus_tag="VF589_03480"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX8256669.1"
                     /translation="MNIHEYQAKELLARFGVPVPDGHAAMSVAEAVAAAEKLPGPLYV
                     VKSQIHAGGRGKGKFKELGPDAKGGVRLARTLDEVRAAASEMLGNTLVTIQTGAEGKQ
                     VQRLYVTDGVDIEKEFYLALLVDRSTGRIAVVASTEGGMDIETVAHDTPDKIETITID
                     PATGLMPHHGRAVAAALELDGDLAKQAQQVLARLYEAFVGTDASQIEINPLAVTGDGK
                     VMVLDAKVSFDSNAMFRHKDLAGLRDESEEDPMEIEASRYDLAYIKLDGDIGCMVNGA
                     GLAMATMDIIKLNGAEPANFLDVGGGASKEKVTAAFKIILSDPSVKGILVNIFGGIMR
                     CDIIADGIIAAAKEVNLSVPLVVRLEGTNVEEGKAILAQSGLAIVPANDLGDAARKIV
                     AAVREAA"
     gene            8407..9156
                     /locus_tag="VF589_03485"
     CDS             8407..9156
                     /locus_tag="VF589_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX8256670.1"
                     /translation="MKLLVPVKRVIDYNVKPRVKADGTGVDLANVKMSMNPFDEIAVE
                     EAIRLKEKGVATEIVAVSIGPAQAQETLRTALAMGADRAILVTTDQDVEPLAVAKILA
                     KIAAEEQPGMVILGKQAIDDDSNQTGQMLAALLGWPQGTFASKVEVEGESVNVTREVD
                     GGLETVRLKLPAVVTTDLRLNEPRYASLPNIMKAKSKPLAHKTAADYGVDIAPRLTTL
                     GVVEPAKRSAGVKVGSVDELIDKLRETGVVG"
     gene            9153..10082
                     /locus_tag="VF589_03490"
     CDS             9153..10082
                     /locus_tag="VF589_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX8256671.1"
                     /translation="MKTLVWAEHEGGQLKDATLAVVTAAAKLGEVHLIVAGHGVGAVA
                     EAAARIAGVGKVHVADDAAFAHGLAENVAPLIVDQMGHHDAFLAPATTTGKNIAPRVA
                     ALLDVMQISDILSVEGPDTFTRPIYAGNAIATVRSSDAKKVITVRGTAFEKAATTGGS
                     AEIVPIGSAGDAGLSSFVGQEVAHSERPELTSANIIVSGGRALGSSEQFHALIDPLAD
                     KLGAAVGASRAAGDAGYAPNDYQVGQTGKIVAPEVYIAVGISGAIQHLAGMKDSKTIV
                     AINKDEDAPIFQVADLGLVGDLFKLVPELTEKL"
     gene            complement(10105..11616)
                     /locus_tag="VF589_03495"
     CDS             complement(10105..11616)
                     /locus_tag="VF589_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8256672.1"
                     /translation="MIGLRIAAAAAALLAAAAPAEAQVAKWVERNRVPILREYVDLLA
                     IPNVASDTPNIRRNADRLVQMMVKRGLSPRLLEEGDPAVPPAVYGEWRVPGAKRTLVI
                     YAHYDGQPVTPADWKSTQPFTPVLRTARLDRDGKVIPLPDAGAAIDPEWRVYGRSSSD
                     DKLGVMAILAAVDALRAEGKMPAFNLKILLEGEEEAGSPHLEKILRKHAALLKSDGWV
                     IVDGPTHPTGARQVVLGVRGDVNVDVTVYGPARPLHSGHYGNWAPNPAMMLSRLLASM
                     KDETGRVTIAGFYDDVTPLTAAERAAIAAVPSADAQLRDELGLGWTEGGGRRLLELTH
                     EPSLNINGIRSADVGEAARNVIPTVASATLDLRLVKGNDHRRQVERLKEHIRRQGYTL
                     FDREPTLAERRRFPRVATVIAQTGYNAERTPLDHPLARSVAAAVAETGPSVVLPSLGG
                     SLPLYLLRETLGAPSVTLSLANHDNNQHAEDENLRLANLWGAIETIAAVMRME"
     gene            11736..13370
                     /locus_tag="VF589_03500"
     CDS             11736..13370
                     /locus_tag="VF589_03500"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8256673.1"
                     /translation="MRLKAFRTGLLLPLLFATPAAAADIQASRIKEHVRILSSDEFAG
                     RGPTQAGEAKTIDYLAKQFAAMGLQPGGDNGSWFQDVPLVRGERVGPAVISVEVGGAT
                     VPLGTGEQVTASSGVVGRTALAGAPMVFLGYGMVAPELGWNSFEGADLEGKVAVFLAG
                     DPDFEAPQPAGFGGRTLVYGGRFGAKAAAAAKAGAAAVLVIHETAPASYPWSQVAGNE
                     GAPTFGLRPAPGATPFGLTGWLHRDAAVDLFRRAGLDFEALKLRARDKSFRPLPIGDA
                     RLSAELTTRSTEVISRNVVARLPGSRYPNETVIYGAHWDANGIGAPDAEGDTIRNGAV
                     DNATGTATLLEVARAFARGKRPDRSVLFIGYTAEEKGLLGAEYYAARPLYPLETTVAV
                     LNLDPHVVIGKARSLDLIGGGRTELEQDLVRVAGKHGLRVDEERSPEAGWYFRSDHFA
                     FAKKGVPALAYRAGRDLVKGGMAAGNAAIEDYNANRYHATTDEYDPAWDMAGAEQEGQ
                     VAYKLGLELANSRRWPGWNEGVEYKPIREASAAARK"
     gene            complement(13532..14536)
                     /locus_tag="VF589_03505"
     CDS             complement(13532..14536)
                     /locus_tag="VF589_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX8256674.1"
                     /translation="MRTFFDPRQLAHAPSQELHNGGYTAYAEHPGRVEAMLAAVGATE
                     APADHGEAPILRVHPQDYVDFLKRAHGEWLQAGRPGDAHGYVWPVVGRRPLALARIDA
                     LLGQYSYDASTPIAAGTWESAYWNAQTALSALDAVLGGERAAFALCRPPGHHAGADYM
                     GGYCYLNNAAIAAEAGLAAGRKRVAILDVDYHHGNGTQDIFYERGDVLFVSIHADPKF
                     DYPFFWGHADEAGLGDGEGATLNLPLPRGTALAGFDRALDTALERIAGYAPGLLICSY
                     GADTFAGDPISFFDLQTADYAAIARRIAGLGLPTLVVMEGGYAVDALGDNVASFLSGF
                     "
     gene            complement(14544..15836)
                     /locus_tag="VF589_03510"
     CDS             complement(14544..15836)
                     /locus_tag="VF589_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256675.1"
                     /translation="MKLLSLAAAWLLLLAGCVSDRTADDSLDEIAADYVQMTLEIGER
                     EPGYVDAYYGPAEWQDAAKAAPRSLQDLATEAVTLTSRVNAVPAAGLGPLEQRRRAFL
                     LAQLKAAHTRLRMLRGEKLSFQDEAEGLFGVRPELKPLASYDPVLARIERLAPGSGPL
                     WQRVDAFQEHFTIPKERLKPVFDTAIAECKRRTAANIALPEGETFTMEFVTGKSWSGY
                     NWYKGKLSSLIQINTDLPIRISRAVDLGCHEGYPGHHALNMLLERRLMRERGWVEYSV
                     YPLYSPQSLIAEGSANYGIDLAFPGAERLLYETQALYPLAGLPTRDAGRYLGLLEAVK
                     DLAGARFTIAADYLEGRIDRAEAVELTQKYQLVSKKRAEQSMDFVDQYRSYVINYGLG
                     QDMVRAHVEAAGPTPKARWAAMERVISEPTLPSDLRGQ"
     gene            complement(15833..17116)
                     /gene="nhaA"
                     /locus_tag="VF589_03515"
     CDS             complement(15833..17116)
                     /gene="nhaA"
                     /locus_tag="VF589_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX8256676.1"
                     /translation="MAEARKPTGGRSALRQFLKSEAAGGILLMGAAAAALIVANGPLA
                     DLYHHALHDPVSPALTPKLGPMTIHLWINDGLMAIFFLLVGLEIKREFVDGRLSTWSQ
                     RRLPFLAAAAGMVVPAGFYLLIVAPDPALTSGWAIPAATDIAFAIGVLALLGKRAPTS
                     LKLFLTTVAIVDDMGAVAIIALAYTASIATLPLGIAAAILLVMYFLNKSGVTALWLYM
                     LLALGLWYAVLLSGVHATIAGVLAAVMIPVIKTPGAPDAPESPLHRLEHAITPVVAFL
                     IVPLFGFANAGVPLDDVGLDQLFAPLPLGIAAGLFVGKQVGIFGSVWLAVRLGFATRL
                     RGATWLQIYGVSLLCGIGFTMSLFIGALAFPGREELVEEAKLGILLGSLLSALVGYAV
                     LRLAPQHCRFEEAESQQGGEIDVDGDVVSLEEKRA"
     gene            17213..18703
                     /locus_tag="VF589_03520"
     CDS             17213..18703
                     /locus_tag="VF589_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEX8256677.1"
                     /translation="MNELKPATITASRHPFLQWWSDHVMASVDHREVVEKVQEDAGWS
                     SHYVFMTLMSAGIAVLGLLLSSPAVVIGAMLISPLMGPIIGLGFALATFDSAEMKRSA
                     AALALGVALAVFFCAFVVLFSPLQTVTDEIASRTRPNLFDLLVALFSGLAGTYAMIRG
                     RHGTIVGVAIATALMPPLAVVGFGLATANWPVFWGASLLFFTNLMTIAVSAAILARIF
                     GFAANLSPRQTGLQATLVVAIFVALGVPLGLSLKQIAWETLASRQAREVVAGHFGEEG
                     RLSEISVDYDARPIAVEATVLTPRHRPGAERETAASLEKMLKVPVKVTIDQFRVGSEG
                     EAEAAALAAARGSAAERARSRLAERLALVAGVSPDEVLVDRANSRAQVRAAALPGADL
                     ASYRALEARVAASERGWTIALVPPAAPLPAIAFEDGQPDGAALATAIWAARRLNLPVG
                     VSGGSDTQAEAVITALREAGVDARRSEGGGGGVELRWLAPRVAAEE"
     gene            18787..20169
                     /locus_tag="VF589_03525"
     CDS             18787..20169
                     /locus_tag="VF589_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEX8256678.1"
                     /translation="MVGLEPLFAGLDFVMREAALFAATSFLVLGAGDVLVDLLWLALV
                     VRRGVAGDRLIRASDLAGAGQPGTIAIFVPAWDEAEVIEPMLRHALLRLRHEDFVIYV
                     GCYPNDPATIAAVARVGDPRVRLVVGPLPGPTTKADCLNRIWAAMVLDERLKRRRVRA
                     IVLHDAEDVVDPDELFVFDVLIGRFDMVQLPVFPLVDENSRWIAGHYADEFAESHGKE
                     MVVRQAIGAALPSAGVGCAFAREALEDIAAARGGGPFDAGSITEDYELGLRLRALGGR
                     AAFVRLPGRDGRSLVVTKEYFPATLEAAVAQKARWMTGIALAGWDRLGWSGSLAERWM
                     RIRDRQSLLAALALGAGYVAGLIWTMLTLRQIFLGIDADPVAPLLALFLQINLAMLGW
                     RLMVRFAFVTHAYGWREGLRSLARIFVGNIIAMLAARRAILGYIAMRRTGSARWDKTA
                     HAFPVEMPAE"
     gene            20415..21221
                     /locus_tag="VF589_03530"
     CDS             20415..21221
                     /locus_tag="VF589_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256679.1"
                     /translation="MVRSSSATGDAPDPLDPVTTFAATFGAADTLPAPAPASLPVQAA
                     APLAVPPFAPDRPPGRWSASGWAFLRRGGEPSLASGGTLGGSQIGARILYRLNRDAAR
                     PLSLAARFYSPLESRGAEASLGVEWQPLAGVPLRLLAERRQALDDRGRSAFSLLAHGG
                     VSDRRVVGPLLLDAYAQAGIVGLRSADLFADGSAQIGIPIGREGRLKVGAGLWGAVQP
                     GLSRLDAGPQASYRMRLGGAAVRVAAEWRFRMAGDAAPASGPALTVATEF"
     gene            21267..22184
                     /locus_tag="VF589_03535"
     CDS             21267..22184
                     /locus_tag="VF589_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8256680.1"
                     /translation="MDIYLPIAGLSVNALVIIGLGGVVGLLTGMLGVGGGFLTTPILI
                     FYGIPPAVAVASATTQITGTSVSGLLAHRRRRGVDYRMGGVLVAGGILGALAGGGLFR
                     LLQDSGQIDTVISLLYVILLGSIGVLMAKESATALDIIKPTARAKKKAARRHNPLIAA
                     LPMRWRFYQSGLYISPLAPLALGFVSGMLTVLLGVGGGFIVVPAMIYLLGMSTMAVVG
                     TSLLQILFVTAATTLVHATTTKSVDIVLAVLLLVGSVIGAQIGVRLAQKAKPELLRMF
                     LAIVVLAVALRMALQLGWRPAEIYTVQSL"
     gene            22181..22942
                     /locus_tag="VF589_03540"
     CDS             22181..22942
                     /locus_tag="VF589_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEX8256681.1"
                     /translation="MRRLRALLLALALPLTLGATEPLLVPDVSQRKIEIRYSFTGAEL
                     LLFGAIIYPGGRTPKEPADIAVVVKGPAEPILVREKQKVAGIWMNVESERFRSAPSFY
                     AVASSRPLTELVDERMAAIYELGLENLQLSPGAGASPEERRRFESGLIELMRSHQLYV
                     EAPRGVEITGGVLYRARLNIPARVPVGNYTAETFLIQDGRVLAAAVREIRIEKLGFER
                     FVAEAAERWSFGYGLVAVLVSLFLGWGGSAVFRRV"
     gene            23034..24731
                     /locus_tag="VF589_03545"
     CDS             23034..24731
                     /locus_tag="VF589_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX8256682.1"
                     /translation="MTDMMNYQSFQDDEQGETRRPTIKQVELPPPTTRHEQIGKIVQV
                     SGGGARIQMRSARLRELALETDPSVAMSGQVGSQVKLESGRRWLLANVRNLCVTDSEG
                     DVIVAEIDFLGEGDEDEQTGRLVNFKRGVTGYPIPGNRVFPVTSADLKQMFAADERPH
                     IEIGTVYPTKDVRGALYVDAMLGKHFALLGSTGTGKSTSAALIMHRICDMAPEGHIVM
                     IDPHGEYSAAFKGYGELFNVDNLAMPYWLMNFEEHCEVIVTSSGADRQRDADILAKCL
                     LHARSKNRVAEGLTKLTVDSPIPYTLTDLTTAITNEMGLLNKATDTAPYMRLKTKLDE
                     LKADPRYSFMFSGMLVADSMTSFISKIFRLPAKGKPISIVDVSGVPSDITSVVVAVLS
                     RLVFDYAIWSRNELQRPVLLICEEAHRYVPSDKTSSGQAVRKILERIAKEGRKYGVSL
                     GLITQRPSDLAEGVLSQCGTIIAMRLNNERDQAFVKSAMPEGARGFLDVIPALRNREC
                     IVCGEGVSIPIRVSFDDLERDRRPASSDPAFSELWKQTGDEEAMVARVVKRWRAQGR"
     gene            complement(24914..26455)
                     /locus_tag="VF589_03550"
     CDS             complement(24914..26455)
                     /locus_tag="VF589_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8256683.1"
                     /translation="MYQFNDFSAGAGGGTAALSLDAALLRARPAPTSHNRLHGLRARI
                     GELDLVVDLGVRIGSREWLRGAATCLALCYGAWSFAPSASALVGASPAPLPEAQWEEA
                     RALAISPLALGADTGRRMSPTDAVAPLLDTPERPSVDLFATLGRGDGFTRVLERAGVA
                     RAEADAIGAMVAGAVPLDEIRPGTMLDITLGRRPNKSVARPLEALAFRARVDLKLEIA
                     RVDGRLAMTPIPIAVDNRPLRIQGLVGSSLYRSARAAGAPAKAVEAYIRAIAGQINIG
                     SVEADDRYDIILEHRRAATGETETGKLLFAGLRRSGGKDLQLMQWEQGGKTQWFEATG
                     VGKASGMLQRPVPGAVSSNFGMRRHPILGYSRMHKGMDFRAGYGTPILAATEGRISAA
                     GWAGGYGRQVRIRHGGGLLTSYSHMSRITAQPGAFVKQGQVIGYVGSTGLSTGPHLHY
                     ELYRNGVPINPASVKFTMRAQLSGEELAGFRSRLHGLLSVPVGAVRGEVQQAKAEPGK
                     AAGRT"
     gene            26660..29176
                     /locus_tag="VF589_03555"
     CDS             26660..29176
                     /locus_tag="VF589_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX8256684.1"
                     /translation="MSDFARAPLAAILGPTNTGKTHLAVERMAAHSSGMIGFPLRLLA
                     REVYDRVVKLKGPKQVALITGEEKILPKDARWFLCTAESMPMERDFAFVALDEAQLGA
                     DPERGHVFTDRMLHARGREETMILGSEALAPMVRALLPKAEIIGRPRFSTLSYAGATK
                     LSRLPPRSAIVAFSAEQVYAVAEMLRRMRGGAAVVMGALSPRTRNAQVAMYQAGEVDY
                     LVATDAIGMGLNMDVAHVAFAGLSKFDGRRRRRLTPAEMAQIAGRAGRHQRDGTFGSL
                     TLEGESAAEFRPEEIDAIEGHRFAPLDHLFWREGDPDFRSVDALIRSLERRPDRACLR
                     AAPEAVDLAVLKRLAEDPEVRARATGYDRVRTLWSACGLPDFRKTGPEHHARLVARLY
                     THLSEGNGRIPQGWYAEQVARLDNVQGDIETLADRIAGVRTWAYIAHRADWLVSPEEM
                     AERTRAVEEKLSDALHERLTQRFVDRRTSVLLRDIGRKGAGEFPVLVDEQGEVTVGSF
                     PIGRMEGFAFEVDPTARHADRKMLLATAERRLGGEYEKRAAALVADTDDHFTLRTEPG
                     QPVAILWRGHEVARLGPGKNLLSPRVLLDRRIERLSERGREAVIERLKNWVRHQVERA
                     LGPLRAAGAAAQDAAAPSAVRAIVAMLVDDGGIVARDAVAQPLAQLDREQRRHIARLK
                     VRIGTLDLFMPDVLKPEARRWRTALRAAASGAAMPELPPHASVVLPTPEDERRLLLAR
                     LGFRALGPQMLRVDLVERLARHAHEARAGKHGQVVDETLATSLGLQPQAIARLMKEIG
                     FRPAQSEAGWIWTGRERRRPATAAGRGHAFAALAGLRRGG"
     gene            29136..29468
                     /locus_tag="VF589_03560"
     CDS             29136..29468
                     /locus_tag="VF589_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX8256685.1"
                     /translation="MPSPRLPGSAGVADSLRLDKFLWFARIVKTRALAQQLAEQGRLR
                     LGGRVIDRAHAPVRVGDVLSFAVRGNVRVLRVEALPTRRGPPAEARALYSEVVPADGT
                     LTSRGAGD"
     gene            29495..29833
                     /gene="fdxA"
                     /locus_tag="VF589_03565"
     CDS             29495..29833
                     /gene="fdxA"
                     /locus_tag="VF589_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX8256686.1"
                     /translation="MTYVVTEACIRCKYMDCVEVCPVDCFYEGDNMLVINPSECIDCG
                     VCEPECPAEAILPDTEVGLEKWLEVNATYSAEWPNITRKRESPADADEHKGEAGKFDK
                     YFKPDPGQGD"
     gene            30094..30630
                     /locus_tag="VF589_03570"
     CDS             30094..30630
                     /locus_tag="VF589_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX8256687.1"
                     /translation="MAAKALNFDVGDYVVYPKHGVGRVIELQSTDIAGMQLELYVLRF
                     EKERMTLRVPTNKAESVGMRKLSSDKTMQEALATLKGKPKVKRTMWSRRAQEYEAKIN
                     SGDLVSIAEVTRDLFRADDQPEQSYSERQIFEAASSRLARELAAMEEVDEPKALEKIL
                     DILNKAAAIYNKEKAVEK"
     gene            30795..31526
                     /locus_tag="VF589_03575"
     CDS             30795..31526
                     /locus_tag="VF589_03575"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX8256688.1"
                     /translation="MRSALILVSTLALAACNMAGAEEGEDRSAAPAARQSYNVGSFDS
                     VSLGGPYDVVVQVGPAASVRAEGDAEEIAQMVVEVKNGSLEIRSAKRSGNFTIGFSRD
                     RNVTVYVTAPSLRAAAIGGSGDMRIDKVEGGEFAASIGGSGDMTIAAMRVGEASFSVA
                     GSGGIRAAGTAERSSVSIAGSGDVIVDGLKSRSASVSVVGSGDVQANAAETADVSIMG
                     SGNVKIAGGARCSVNKVGSGDVSCG"
     gene            31575..32285
                     /locus_tag="VF589_03580"
     CDS             31575..32285
                     /locus_tag="VF589_03580"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX8256689.1"
                     /translation="MNRIMLSAAALALASLAVAVPAAAAERRFTVTQFDRIQVDGPFA
                     VTVTTGRATGALATGDARAIDRVSIEVQGRVLKVRPNRSAWGGYPGEGAGAVAIAVST
                     HELRGVAVNGSGTVAIDKARAMRFDLSVSGSGVVELPNLEADRLVLGLLGSGRITLGG
                     KAKTVRATIQGTGDLAAAELIAEDAELNADSSGKIALGVKRAVKVTATGAGDVEIAGS
                     PACTVKALGSGRVSCGAD"
     gene            complement(32310..32714)
                     /locus_tag="VF589_03585"
     CDS             complement(32310..32714)
                     /locus_tag="VF589_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEX8256690.1"
                     /translation="MSSLHISKVAVGCASFDALRKRQQSRLSAGIVPITTRFRPKRAE
                     ELIGGSIFWIVKHRITARNRIVGFAEAEERRTLIQLDPELVPVRAWPKRAHQGWRYLA
                     ADDAPLDFDGSDLGIAAMPPGLAIKLSALALI"
     gene            complement(32756..34171)
                     /gene="mgtE"
                     /locus_tag="VF589_03590"
     CDS             complement(32756..34171)
                     /gene="mgtE"
                     /locus_tag="VF589_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX8256691.1"
                     /translation="MSDSDIEIADGAPPSAADRLDEDDRLKPQFVSQVMDRVEAGDTD
                     GARALVEPLHPADIADLFEIVDRDERRALAAALADLLDGDVFAEMNDWVREELIDALE
                     PHQVAEIAGELETDDAVAIIEDMEEDDQRAVLRALDPDDRAAIEEALTFPEESAGRLM
                     QRDLIAVPEHWNVGQVIDYLRAGEDLTTDFWEIYIVDPQHHPVGTVMLSWVLRTPRHV
                     AMADLMQREQTLIPVDMDQEEVALRFQKYALISAAVVDQSGRLVGMITVDDIVHIIQE
                     EASEDVLLLSGAGDEGDINQPIRDSYRARVRWLVANLFTALVASSIIAAFGGAIEQMV
                     ALAVLMPIVASIGGNAGTQTMAVAVRALAMNQLTRSNSLRTIWREVRLALLNGLTIAV
                     LVGIGAGVVFSNPALGGVIAVAMLINIAIAGLAGVLVPLALERLDQDPAVASSVFVTM
                     ITDSMGFFAFLGLAVASGLVG"
     gene            34310..34783
                     /locus_tag="VF589_03595"
     CDS             34310..34783
                     /locus_tag="VF589_03595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8256692.1"
                     /translation="MANELNTLVLSLDTGNDGGGDVVIRLRPDLAPGHVERIKSLVQD
                     GFYDGVVFHRVIPGFMAQGGDPTGTGTGGSKQPNLKAEFSAEPHVRGTCSMARASSPD
                     SANSQFFICFDDAGFLNGQYTVWGQVTEGMEHVDALPVGEPPRKPGRIVKATLRD"
     gene            34783..35538
                     /locus_tag="VF589_03600"
     CDS             34783..35538
                     /locus_tag="VF589_03600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8256693.1"
                     /translation="MRPVTLVTGASSGLGAEFARQCSRRGDELVLVARRKDRLEALAA
                     EIGGNAHVIAADLAAPEAPERLLADIEALGLTVDTLINNAGFGLAGPFAKLALDRQAE
                     MIELNVASLTRLCHLVLPKMLGRRQGAILNVASTGAFQAGPGIAVYFATKAYVLSFTE
                     ALHQELKGRGIRVSALCPGPTRTEFGEVAGFTSASFDRFAADAASVVTAGLHGLDRNK
                     AVVIPGLTNKLSAQSNRLIPRAAMRRIVGALKL"
     gene            complement(35535..36452)
                     /locus_tag="VF589_03605"
     CDS             complement(35535..36452)
                     /locus_tag="VF589_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8256694.1"
                     /translation="MRRLPPLTALEAFIQVARLGSVKAAAEALALSSPALSRRLQAFE
                     RFVGHPLFERRHQALHLNPDGERLLAEIASSLDALALAMERATGEADFMRLRLAVLPL
                     FASQRLMPHLSELRTLHPDLHIDIDTGAHGLTRLDEGLDAAIAIATEIDPNLYARRID
                     SNCVIAIGSHELRDGPQALTHPAQLAGATIFLHRDLPDTFDYWREAVGLPELQPAAID
                     HFDSGQLILDAAAQGLGVAFMLESHLKEAHDDRLVQLFDVKVESPYSYWFACRRSALA
                     RRPVRIFHDWLFEWVEGEEARPPAGAAAP"
     gene            36792..39098
                     /locus_tag="VF589_03610"
     CDS             36792..39098
                     /locus_tag="VF589_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX8256695.1"
                     /translation="MADEEKEPQRLQVANMRPDDSGRGLARLPRTVMTSLGLSEGDVV
                     ELTGKRATPARAVLSYPEDDGLSIIRLDGLQRANAEIGSGDFVTIRKTESKPAQRVVF
                     APAQKNLRLQGSANALKRSFGMKPVTAGDIVATTGQQAINRGDIPPELRQMLNAPAYA
                     LQEIRLNVVSTTPKGIVHIDQNTEVELRPEYTEAKDQRRADVTYDDLGGIGATIDQLR
                     EMVELPLRYPEIFERLGVDPPRGVLLHGPPGTGKTRLARAVANESDANFFHIAGPEIM
                     GSAYGESEKRLREIFQEAAKAAPSIIFIDEIDSIAPKRGQVSGEAEKRLVAQLLTLLD
                     GLEPRQNLVVIAATNRPEAIDEALRRPGRFDREIVVGVPDERGRREILAIHTRGMPLA
                     DGIGLAELARRTYGFVGADLAALTREAAIEAVRRIMPKINLEEGTIPAEVLDNLSVTR
                     DDFEEALKRVQPSAMREVMVQMPTVRWEDIGGVDEARERLREGVELPLKDPDAFRRLG
                     IRPAKGFLLYGPPGTGKTLLAKACAREAEANFIATKSSDLLSKWYGESEQQIARLFAR
                     ARQVAPTVIFIDELDSLVPARGGGMGEPQVTERVVNTILAEMDGLETLQSVVVIGATN
                     RPNLIDPALLRPGRFDELIYVSVPDRAGRRRILGIHTAKMPVAADVDLDDLAARTERF
                     TGADLEDLVRRAGLTALRETLSATIVNADHFEAALKETRASVTPEMERDYAEIKAKLK
                     QEAMGSGNIGFISPGMLTPRGPKGGNEL"
     gene            complement(39061..40338)
                     /locus_tag="VF589_03615"
     CDS             complement(39061..40338)
                     /locus_tag="VF589_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8256696.1"
                     /translation="MSGYSLSRGRFALLFAIMLVAAAGNTAMQSLMPSIGRELGIPDL
                     WVAVAFSLSAVIWVVTAPHWARLSDRRGRRALMRLGLYGFIISMLICGSVLAAGLKGW
                     LPPTIVFWIFVLGRAIYGGWGSASPPAVQAYVAARTEGEQRTAALAAIASSFGLGTIV
                     GPALAPLFIFPPLGLSGPLIVFAAIGALVLVAVVTRLPDDSPSGPARGRVADYPSIGG
                     AGGGPGSVQGTEPAEPLRWRDPRVLAWHMIGIVGGHGHAALLGVIGFLVIDRLKLPLD
                     EAQQWIALVLMVGALATLLAQWWLIPQFALAPRALVMWGSLLSAVGVVLTGVSQGIYG
                     ITLGFALASLGFGFFRPGFTSGASLSVTPAEQNAVAGMVTSVNGVAFIAAPAAGVALY
                     GIWMPLPFLVTGALMLGLALWVKVRFRPSAPAV"
     gene            40536..41153
                     /locus_tag="VF589_03620"
     CDS             40536..41153
                     /locus_tag="VF589_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8256697.1"
                     /translation="MTFSAEQAQRDRAFTRRAGGGPLWQFLVGFIKHPVMVGSVIPSS
                     KVLIDKMLAPVDWDNCKLFVEYGPGVGTFTQQILDRLAPDATLLTIDTNPDFTDYLNG
                     KFTDSRLEAVTGSAASVRKIMADRGFESADYVLSGLPFSTLPPGIGPKIASETAAALR
                     PGGAFLVYQFSPKVRDFIAPHFERIDKGFEWVNIPPATLFWAWKD"
     gene            complement(41166..42341)
                     /locus_tag="VF589_03625"
     CDS             complement(41166..42341)
                     /locus_tag="VF589_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX8256698.1"
                     /translation="MAEHDPVMQAPDGALMVEDNWLSLLPDGPERLEALIALIEGAQR
                     SLRILYYIFLDDNSGRRVRDALLAAAERGVKVTLLVDGFGSDSAKASFFQPLTDGKVD
                     FCRFAPKLGRRYLLRNHQKLALADDTCVLIGGFNVSDDYFGDIADGAWRDLGLRVEGP
                     GVVCLAHYFDALARWTKRPQGRIRELRHMLKQHSEDVGPLRWLYGGPTRRLSPWAQSV
                     KADMLRACRLDMIAAYFAPSRTMLRRIYGIGERGCARVVTAAKSDNGATVGAARHTYS
                     RLLRRGVEVYEYQATKLHTKLIVIDDVVHIGSANFDMRSLYLNLEMMLRIEDAAFAAA
                     MRRFIDGEVANSLQITREYHRRQRTWWNRVKWGIAYFIIATMDYNVSRRLNFGGTVR"
     gene            42461..42802
                     /gene="rpoZ"
                     /locus_tag="VF589_03630"
     CDS             42461..42802
                     /gene="rpoZ"
                     /locus_tag="VF589_03630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX8256699.1"
                     /translation="MARVTVEDCVDKIPNRFDLVLLAAQRARQISGGADLTIDRDRDK
                     NPVVALREIAEQTVKSKHLEEAMVQNLQRVQIDDDDAPDEVGSLSASAEALRLTAAAP
                     PRNTNMGADYE"
     gene            42799..>43316
                     /locus_tag="VF589_03635"
     CDS             42799..>43316
                     /locus_tag="VF589_03635"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8256700.1"
                     /translation="MIGEGAPPAGPAFHIFDTAIGRCALVWRGGALIGSALPGPRLDE
                     SLARRFPGAAPADPPPFVRSAIDRIAALLGGDRIDFAGIPLDLGEAAPFERAVYAAAC
                     AIPAGEVRTYGELAAAIGTPGAARAVGRALGRNPLPILIPCHRILAAAGKSGGFSAPG
                     GAATKFRLLALER"
BASE COUNT         6827 a        15059 c        14639 g         6791 t
ORIGIN      
        1 tctgccggga cacgaaagcc agctcgagaa gaagcccgag tgggaaccgc gctacccggg
       61 ctccggccgc ctcgaaggca aggtcgctct gatcaccggc gcggacagcg gcatcggccg
      121 cgccgtcgcc gccttgttcg cgcgcgaggg cgccgatgtc gcgatcctct atctctgcga
      181 gcatgacgac gccgccaaga cgcgcgagat cgtcgaagcc gagggccggc gggcgatcgc
      241 cattgccggc gacgtcggcg acaaggactt ctgcgagcgc gccgtcgccg agacggtggc
      301 gaagctcggc cggctcgaca tcctcgtcaa caacgccgcc gagcagcacc ccgacaccga
      361 gatcactgac atcaccgaag agcagctgaa gcgcaccttc cagaccaaca tcttcggcta
      421 tttcttcatg gtccaggcgg cgcgcccgca ccttcaggag ggcgcggcga tcatcaactg
      481 cacgtcggtg acgatgtaca agggatccaa ggagctgctc gactattcgg cgaccaaggg
      541 cgcgatcacc gccttcaccc gctccttgtc ggaaaatctg atcgacaagg gcatccgcgt
      601 caacgcggtc gcgccggggc cgatctggac cccgctcaat ccgtcgggcg gatcgacgcc
      661 ggagaagatg gagcatttcg gcgaggacgt gccgatgggc cggcccggcc agccgaacga
      721 ggtcgcgccg agcttcctgt tcctcgcctg cgaggattcg agctacatgt cgggccaggt
      781 gctgcatccc aatggcggcg agatcgtcgg cagctgaccg cttctggcgg cggcaattca
      841 tgctatactc gtgccgaggc aggagggtcg gctatgctac ggatcgtcag cggtgcggtg
      901 gcggggctgt tggcggcgtt cgcggccatc atggcgatcg aatatgtcgg ccatgcgatc
      961 tatccgctgc ccgccgacgc cgcccttcgc gatcccgaag cgcccgtgga cgcgctgtcg
     1021 atgccgctgc cggtcttgct gatcgtggtc ttcgcctggt tcgccggggc cctgatcggc
     1081 ggtgccgtcg ccaagcgcat caacggccgg tggtggtcgc cctggctggt cgcgggcatg
     1141 gtggcgctcg gcgggatcct cgccgtgacc atgatgccgc acccgacctg gatgcagatc
     1201 gcggcggtgg cggcgccgtt gctcggcggc ctctttgcca gccacgccgt gtggggcgac
     1261 aggagggcga ccgatggagc gctataagag cttccgcgaa ttctggcctt tctacctgcg
     1321 cgagcacagc aagccgcgca cccgggcgct tcattatgcc ggcacgaccc tggtggtgct
     1381 aatcgcgctg ggcgcgctcg tcaccggcac ttggtggctg ctcgccgcga tgccggtggc
     1441 cggctacggc ttcgcctggg cgagccatgc cgcggtcgag cgcaaccggc cggctacctt
     1501 cacctatccc ggctggtcgc tgaaggcgga tttcctgatg tggtggctct ggctcaccgg
     1561 ccggctgggg cccgagctcg aagcggcggg ggttcggccc aaggcgcgct agaggtcgct
     1621 gatctcgaag ccctcgccgg cggccatacg ctcggccagc accgcgcggt ggcagcgcgg
     1681 cgcgtccttc tcgaagcaca ataaggccgc gcgccgctcg cgcgcttccg acatggcctt
     1741 gacgaaggcg agctcggcct cgggcgtttc gagctgcccc gcatagatgg cgcgcatctc
     1801 cccggtgcgg cccttgcgcg ccgcttcgcg cccggcggcg ggggtgccga gcgggcgcag
     1861 gtggaggtaa tcgatgccgc gctccttgag cgcccccgcc agcgcggcct tggagaagcc
     1921 agggcggcgc gaggcggcca cggcgcgcac gtcgatcagc agttcgatgc cggccgcgct
     1981 cagctcgccg aggaattcgg gaagggtctt gccttcatag ccgatcgtga acagcggggt
     2041 catggtcggc gtcgctagca tcttcgccgc cgccgcgcat cggcagcgac caggaaatct
     2101 gcttgcgcag cgacgcttcg agatcgtcga ggccggcctg ctgggcttcg gcgcgcttga
     2161 ccagcagcct ggccagcacc tgctcccggc tttccgggcg ccagcgcagc cggcggccct
     2221 cgcttacggc tttgaacaga cggtgactca agctcgccat ggggacttcc tgcctaacgc
     2281 ttcgcctgaa gcgccgcggc cccatccccc tgggcgcttc atgggtgaag gggtaggccc
     2341 acccttgtta ccaaaggcct aagcccgcgc ggcgatcagt cgaaaagctc ttcgaggaag
     2401 cttttcttgc gcttgcggta gggctggccg tggcccgtat cgtagccgcc gccatagccg
     2461 ggatgcgggg cgtgccccat ttgcggaggc ggcgggggag cggagcgcgg ttgcggcggc
     2521 ggggccgcgg cggcgggagc gtcggccgcg ctgcgctcga tgatcttgtc gagctcgccg
     2581 cggtcgagcc agaccccgcg gcaggtcggg caataatcga tctcgatatt ctggcgctcg
     2641 ctcatcacca gcggcgtacg gtcgatcggg cacagcatcg gctcggccat gttcattctc
     2701 ctatgtcgct cagcaactag gggcgcgcgg tgccgggttc aagccgaccg gcttctgtcc
     2761 ggaatcgtac gcgccgcaca gtccgtgcac cgcggcgtgc aaagatcgcc aacatttggc
     2821 gggaattgct ggattacggg ggcgctaaac ggccattatg gtgtcctggt caccaggaag
     2881 gccgcttttc cgcctagaac gcaatttggc acggctcctg caaagaggtt ggcatgccgc
     2941 cggatcgccc ggcggcggat aaggggagac cttcaatgac catcaatctt tcgcagttcg
     3001 ccgcttccat cgtcggcgcc gtcttcgccg ccaccttgtt cgtcagcgcg gcggtgggcc
     3061 cggtcggtca gttcatctga ccggccgccc cccggcgggt ccgggaggct gacatgcgca
     3121 gaaccttgct ccactcactc ttcttcaagc tgcagatgct gctggcgcgc ccgcacaggc
     3181 cggccccggc gatcgcccgg ggcggcgccg ccgccggcgg aacttaatct gcatcagcga
     3241 attccgtttc gaacgcggct cggccggcgt taatcgaaaa ggggaattcc atgcgaaatt
     3301 tactcaccat cgccgctggc ggcgccatgc tcatgctgac cgcctgcggc ggccagggcg
     3361 acgacgcgct cggcgataac gtcgcagacg ctgccgagaa ccgcgccgac gggctcgagg
     3421 atgccgccga caatgcgagc ggtgcccagg aggattcgct cgaggcgcag gcggagatta
     3481 cccgcgatac cggccaggcg caggaggaag cgatcgacga cgccgacgtg gtcgtcaaca
     3541 accagtaatt cagatcggca ggcggagctg cggcggctcc gcctgttcgc cgccgccgag
     3601 gccggagagg gtgaggccga ggaggcggat gcccttttcg gccgggagca ggccgcgcag
     3661 cagggccttg cccgtttcga cgaagctgcg cccgtccgcc accggcgcgg gcagcgactg
     3721 ggcgcgggtg acgatcctga aatcctcata tttgactttg agcgtcaccg ttcgcccggc
     3781 gatgccgcgc tcggcgatcc gttcccagac gtggaagccg atccggtcga gctcagccag
     3841 caggtcggcc tcgtcgcgta ggtcggcgga gaaggtgtcc tcggccccga tcgacttgcg
     3901 ctcgcggtcc gccctcacct cgcggtggca gatgccgcgc gcgaggttgt gataatattt
     3961 gccggcgctg ccgaaattgt ggacgaggaa ttcgaggctg accgcgcgca ggtcggcgcc
     4021 ggtctcgatg ccgagccgcg ccatcttctc ggcggtcttg gggccgatgc cgtggaagcg
     4081 cttgaccggc agccgcgcaa cgaaggccgg cccctgctgc ggcgtcacaa cgcacagacc
     4141 atccggcttg ttctggtcgg aggcgagctt cgcgatgaac ttgttgtagc tcacccccgc
     4201 ggaggcggtg aggccagtct ccgccttgat cctcgcgcgt atctcgcggg cgatgtcggt
     4261 ggcgatcccg atccccttct gatcctcggt gacgtcgaga taggcttcgt cgagcgagag
     4321 cggctcgacg tgcggcgtgt aatccaggaa gatcgcccgg atctgctgcg acacggcgcg
     4381 gtaggcgtcg aagcgcggcc tgacgaagat cagctccggg caaaggcggc gcgcggtgac
     4441 cgccggcatg gccgaacgga cgccccattt gcgagattca tagcttgccg ccgcgacgac
     4501 gccccgcact ccgccgccga cggcgagcgg cttgcccctg agctccggat ggtcgcgctg
     4561 ctcgaccgac gcgtaaaagg cgtccatatc gacgtggatg atcttgcggg ccggcgcggc
     4621 gtcgttcacc gcttgttcct agcgcaagcc gccgcggttt agaagctgaa gctgagcccc
     4681 acggcggcga ttttcttctc ttggccgcgc acgtccctca tcggctggat gcgcctgaat
     4741 tccggctccc gccgcgaata acgattcacc gagaacaggc cgatgccgat cgtggcgttc
     4801 ggcgccacct cgacgctccc gaccagcccc ttgcggattg cgggctcgcc gtcctgctcg
     4861 ctataatttt gggtggtgac actgaacccg gagccctggg aagggaacag cccggcgccc
     4921 ggcgcgagat aggcggggcc ctgatcgggc gggccgagcc gctgggcggc gagcggacgg
     4981 ctgtcgaccg gctgctgggc ctcggcaggc gcggcgagga cgagcgtcgt cgccagcgct
     5041 gctagtgcaa cgcgttcacg catctccttc attacccctg tttttgtttt gcagctaaag
     5101 cggtggagag cgagtcaaag ttccattcgg tcgcaattcg caaccggttg gtcccgttgc
     5161 ggaacggtct ggagcgaggc gttaaaacgg ggcggcgacg cccgtccggc cgctcggggc
     5221 ccgatccggg aatagtacca aaatgaagct gcctaaggca ggcgcggcaa caagccatgg
     5281 cttcgcgcgg gcggcttctg tagacatctc gccatggtca ggggaaagcg cgacggattg
     5341 ctgatcgccg gcggcggcct cgccggcagc ctggccgcga tcgccatggc caaggcgcgt
     5401 cccgaagtgc cgctgctgct cgtccaggaa ggcgagagct tcggcggcaa ccgcatctgg
     5461 tccttcttcg acgccgacat caccgatggc gaccgctggc tggccgagcc gctcctgtcg
     5521 ggcagctggc cgggctatta cgttgctttc ccggggcata gccgcaagct caaggccggg
     5581 atgagctgcg tccgctcggc cgacgtcgac cggctggtgc gcgagacgct gcgcccggac
     5641 caatatcggc tcggcacccg catcgtcgcc gtccgcgaga acgaactcgt cctgctcggc
     5701 ggcgagcgga tcaaggccga cggagcgctc gacgcgcgcg gggccgccaa cctgtcgatg
     5761 ctcgatctcg cctggcagaa gttcgtcggc cgcgaatatc gccttgccgc gccgcatggc
     5821 ctcgaccggc cggtgttgat ggatgcgacg gtggagcagt cgcacggctg ccgcttcgtg
     5881 cgctgcgtgc ctttggggcc cgaccgcatg ctgatcgagg acatatatta ttcgaatagc
     5941 ccggagctcg ataccgccgc gctcggcggg cggatcgagg cctatgccac caagcgcggc
     6001 tggccgacgg ccgagctcga acaggaagta tcggacgtgc ttccggtcgc tttgggcggc
     6061 gacttcggcg ctttctggcg ggtcggcggc gcgcgggtcg ccaagctcgg cctgcgcggc
     6121 ggcttcttcc acccgaccac cggctcgggg ctcgccgatg cggtccacaa tgcggtgttg
     6181 ctgagccagc agcgcgacct cggcggcgcc gccttgcacg atctgttcga atcgcaggcc
     6241 gcgcagctct ggaagaggcg cgaattctac cgcaccttca acaccatgct gttccgcggc
     6301 gcggctccgc acgagcgcgc cgcgatgttc gagcaactct accggctcga tccggcgctg
     6361 atcgcccggt tccacgccgg ccggcccacg gtgctcgacc gcatgcgcgt cacggggctc
     6421 aagccgccgg cgcccgccaa ggcatcgacg gccgcgccgc gcaagtgagc aaggctcagg
     6481 cgcgaagccg ggccgccagc gtttcgaccg cgtcggccag ccggcgccgc tcctgaaggc
     6541 tccgccagag cagcaaggtg gcgagcgcgg cgatgccgac cagcgccagc aaggcataaa
     6601 agatcgactc gagccagggc tctgcgccgt ccgcctcctg gggcggcggc ggagcgatgg
     6661 tcccgacagc cgctccgggt gacgcaggct gcgctgcggg acccggcgga gcctcgggcc
     6721 ggctcggccc cgccgccggc tccgccccgt ggtccggctg aggcgccggc ggcgcggtct
     6781 cgatcatcgt cgtcgggatg ctgccggagg ggatcgaggc gatggcgccg atgatcgaca
     6841 ggctgaccag gctggcgagc accagcatga tcacgcggct gatccagtcg aacagcttca
     6901 tggcgcttca acccattgcg gccccgcccg ttgcagggcc atgggtccgc gcctgacggc
     6961 aggagcggca gccgagccgc cttgcccatg gcgcgccgac tgtgctttag ggccggcacg
     7021 tttcgcggag gaagagccgt cccatgaata tccacgaata tcaggccaag gagctgctcg
     7081 cccgcttcgg tgtgccggtc cccgacggcc atgccgcgat gagcgtcgca gaggccgtgg
     7141 ccgccgcgga gaagctcccc ggcccgctct atgtcgtgaa gtcgcagatt cacgccggcg
     7201 gccgcggcaa gggcaagttc aaggaattgg gacccgatgc gaagggcggc gttcgcctcg
     7261 cccggaccct ggacgaggtg cgcgcggccg cctcggagat gctcggtaac accctcgtca
     7321 ccatccagac cggcgccgag gggaagcagg tccagcggct ctacgtcacc gacggggtcg
     7381 acatcgaaaa ggaattctac ctcgctttgc tggtcgaccg gagcaccggg cggatcgccg
     7441 tggtcgcgtc gactgaaggc ggcatggaca tcgagacggt ggcgcatgac acccccgaca
     7501 agatcgagac catcaccatc gatcccgcga ccgggctgat gccgcaccac ggccgcgcgg
     7561 tcgccgccgc gctcgagctg gacggcgacc tcgccaagca ggcccagcag gtgctggcgc
     7621 gactctatga agccttcgtc ggcaccgacg cctcgcagat cgagatcaac ccgctggcgg
     7681 tgaccggcga cggcaaggtg atggtgctcg acgccaaggt cagcttcgat tcgaacgcca
     7741 tgttccgcca caaggacctc gccggcctcc gcgacgagag cgaggaggac ccgatggaga
     7801 tcgaggcgtc ccgctacgat ctcgcctaca tcaagctcga cggcgatatc ggctgcatgg
     7861 tcaacggcgc cgggctcgcc atggcgacga tggacatcat caaattgaac ggcgccgagc
     7921 ccgccaactt cctcgacgtc ggcggcggcg cctccaagga aaaggtgacg gccgcgttca
     7981 agatcatcct gtccgacccg tcggtgaagg gcatcctggt caacatcttc ggcggcatca
     8041 tgcgctgcga catcatcgcc gacgggatca tcgccgcggc caaggaggtg aacctgtcgg
     8101 tgcctctggt ggtccggctg gagggcacca atgtcgagga aggcaaggcg atcctcgccc
     8161 agagcgggct cgccatcgtg cccgccaacg acctcggcga tgccgcgagg aagatcgtcg
     8221 cggcggtccg cgaagccgca tgagacccgc accgttcgcc ctgagcctgt cgaagcctgt
     8281 cctgagcgac gccgcaggcg gcgtcgaagg gggctttcct tcttgcggag gaagtgaagg
     8341 ggagggcttc gacaagctca gcccgaacgg ttccgttatc gttccttccg gaggagtcct
     8401 ggaaccatga aacttctcgt gcccgtgaag cgcgtgatcg actacaacgt caagccgcgg
     8461 gtcaaggccg acggcaccgg cgtcgatctc gccaacgtca agatgagcat gaacccgttc
     8521 gatgagatcg cggtcgagga agcgatccgc cttaaggaga agggcgtcgc gaccgagatc
     8581 gtggcggtct cgatcggccc ggcccaggcg caggagacgc tgcgcaccgc gctcgccatg
     8641 ggcgccgacc gcgccatcct cgtcaccacc gaccaggacg tcgagccgct cgccgtcgcc
     8701 aagatcctcg cgaaaatcgc ggccgaggag cagccgggta tggtgatcct cggcaagcag
     8761 gcgatcgacg acgatagcaa tcagaccggc cagatgctcg cggccctgct cggctggccg
     8821 caaggcacgt tcgcctcgaa ggtcgaggtt gagggcgaga gcgtcaacgt cacccgcgag
     8881 gtcgacggcg ggcttgagac ggtgcgcttg aagcttccgg cggtggtcac caccgacctg
     8941 cggctcaacg agccccgcta cgcctcgctg cccaacatca tgaaggccaa gtcgaagccg
     9001 ctcgcccaca agacggcggc cgattacggc gtcgatatcg cgccgcggct gaccacgctc
     9061 ggcgtcgtcg agccggccaa gcggtcggcc ggagtcaagg tcggctcggt cgacgagctg
     9121 atcgacaaat tgcgcgaaac gggagtcgtg ggatgaaaac gctcgtctgg gccgaacatg
     9181 aaggcggcca gttgaaggac gcgacgctcg ccgtcgtcac cgccgccgcg aagctcggcg
     9241 aggtccatct gatcgtcgcc ggccacggtg tcggcgcggt cgccgaggcg gccgcccgga
     9301 tcgccggcgt cggcaaggtc catgtcgccg acgatgccgc cttcgcgcac gggctcgccg
     9361 agaatgtggc gccgctcatc gtcgaccaga tgggccatca cgacgccttc ctcgcccccg
     9421 ccaccaccac cggcaagaac atcgccccgc gcgtcgctgc cttgctcgac gtcatgcaga
     9481 tttcggacat attgtcggtc gaggggccgg acaccttcac ccgcccgatc tatgccggca
     9541 atgcgatcgc gaccgtccgg tcctccgacg ccaagaaggt catcaccgtc cgcggcaccg
     9601 cgttcgagaa agcggccacg accggcggct cggccgagat cgttccgatc ggcagcgcgg
     9661 gcgacgccgg cctgtcgagc ttcgtcggcc aggaagtggc gcatagcgag cggcccgagc
     9721 tcaccagcgc caacatcatc gtttccggcg gccgcgcgct cggctcttcg gagcagttcc
     9781 acgcgctgat cgatccgctc gccgacaagc tcggcgccgc cgtcggcgcc agccgcgccg
     9841 ccggcgacgc cggttatgcg cccaacgatt atcaggtcgg ccagaccggc aagatcgtcg
     9901 cccccgaagt ctatatcgcg gtcggcattt cgggtgccat ccagcacctc gctggcatga
     9961 aggattccaa gaccatcgtc gccatcaaca aggatgagga cgcgccgatt ttccaggtgg
    10021 cggacttggg cctggtcggc gacctgttca agctggtccc ggaactgacc gagaagcttt
    10081 gatccttctc ccttagggga ggggtcattc catccgcatc accgcggcga tggtttcgat
    10141 cgcgccccac aaattggcga gacgcagatt ctcgtcctcg gcatgctggt tgttgtcgtg
    10201 gttggccagc gacagggtga cgctcggcgc gcccagcgtc tcgcgtagca ggtaaagcgg
    10261 caggctgcct ccaagcgacg gaagcaccac cgaggggccg gtttccgcca ccgccgccgc
    10321 gacgctgcgg gcaagtggat ggtcgagcgg ggtgcgctcc gcattgtagc cggtctgtgc
    10381 gatcaccgtc gcgacccggg ggaatcgccg ccgctccgcc agcgtcggct cccggtcgaa
    10441 gagggtatag ccctgccgcc tgatatgctc cttcagccgc tcgacctgac gccggtggtc
    10501 gttgcccttg acgaggcgca ggtcgagggt ggcgctggcg acggtcggga tcacgttgcg
    10561 cgccgcttcg ccgacatcgg ccgagcggat gccgttgatg ttgagcgaag gctcgtgggt
    10621 aagctcgagc aggcgccgcc cgccgccctc ggtccagccc agcccgagct cgtcgcgaag
    10681 ctgcgcgtcg gccgagggca cggcggctat cgccgctcgc tcggcggcgg tcagtggcgt
    10741 gacgtcgtca tagaagccgg cgatcgtcac ccgcccggtt tcgtccttca tcgaggcgag
    10801 cagccggctc agcatcatcg ccggattggg cgcccagttg ccataatggc cgctgtgcag
    10861 cggacgcgcc gggccgtaga cggtcacgtc gacattgaca tcgccgcgca cgcccagcac
    10921 cacctgccgc gcgccggtcg ggtgggtcgg gccgtcgacg atcacccagc cgtccgattt
    10981 caacagcgcc gcatgcttcc ggagaatctt ctcgaggtgc ggcgaaccgg cttcctcctc
    11041 gccctcgagt aagatcttga ggttgaaggc cggcatcttg ccttccgccc tgagcgcgtc
    11101 gacggcggca agaatcgcca tcacccccag cttgtcgtcg gaggaggagc ggccgtagac
    11161 ccgccattcc ggatcgatcg cggcgcccgc gtcgggcagc gggatcactt tcccgtcccg
    11221 gtcgagccgc gccgtccgca acaccggcgt gaacggctgg gtcgatttcc agtcggcggg
    11281 cgtcaccggc tggccgtcat aatgggcgta gatcaccaag gtccgcttcg cgcccggaac
    11341 ccgccattcg ccatagacgg ccggcggcac cgccggatcg ccctcctcca gcagccgcgg
    11401 cgacagcccg cgcttgacca tcatctgcac cagccggtcg gcgttgcgcc ggatgttggg
    11461 cgtgtcgctg gcgacgttcg ggatagcgag cagatcgaca tattccctga ggatcgggac
    11521 ccggttgcgc tcgacccatt tcgccacctg cgcttcggcg ggcgccgccg ccgccagcaa
    11581 agccgccgcc gcggctgcta ttcgcaatcc gatcattgtc attcccttcg gcaccaagcc
    11641 tagcgccgac cccgcggcgt gcatagcgtt cgcccaggct ggacaccggc ggcccggcgg
    11701 cgcagaggcg cggttcgatc gtcagaggta aggacatgag actgaaagct tttcgcacgg
    11761 gcctgctgct tcccttgctg ttcgccaccc ccgccgccgc ggccgatatc caggcctccc
    11821 gcatcaagga gcatgtccgg atcctgtcga gcgacgagtt cgccgggcgc gggccgacgc
    11881 aggcgggcga ggcgaagacg atcgattatc tcgccaaaca gttcgccgcg atggggctgc
    11941 agcccggcgg cgataacggc agctggttcc aggacgtgcc gttggttcgg ggcgagcggg
    12001 tcgggccggc ggtaatctcg gtcgaagtcg gcggcgctac ggtgccgctc gggaccggcg
    12061 agcaggtcac cgcctcctcg ggcgtcgtcg ggcggaccgc gctcgccggc gcgccgatgg
    12121 tgttcctcgg ctacggcatg gtcgcgccgg agctcggctg gaacagcttc gaaggcgcgg
    12181 acctcgaggg caaggtcgcg gtgttcctcg ccggcgaccc ggatttcgaa gcgccgcagc
    12241 cggccggttt cggcggccgc actctggtct atggcggccg cttcggcgcc aaggcggcgg
    12301 cggcggccaa ggcgggcgcg gcggcggtgc tggtgatcca cgagaccgcg ccggccagct
    12361 atccgtggag ccaggtcgcc ggcaatgaag gcgcgccgac cttcgggctg cgcccggccc
    12421 cgggcgcgac cccgttcggc ctcaccggct ggctgcaccg cgacgcggcc gtcgacctgt
    12481 tccgccgcgc cgggctcgat ttcgaggcgc tcaagctgcg cgcccgcgac aaaagctttc
    12541 ggccgctgcc gatcggcgat gcgcggctct cggccgaact caccacccgc tccaccgagg
    12601 tcatcagccg caacgtcgtc gcccggctcc ccggcagccg ctaccccaac gagaccgtga
    12661 tctacggcgc ccattgggac gccaacggca tcggcgcgcc ggacgcggag ggcgacacga
    12721 tccgcaacgg cgcggtcgac aatgccaccg ggaccgcgac cctgcttgag gttgcgcgcg
    12781 ccttcgcccg cggcaagcgc cccgaccgct cggtcctgtt catcggctac accgccgagg
    12841 agaaggggct gctcggcgcc gaatattatg ccgcccggcc gctctatccg ctcgagacca
    12901 cggtggcggt gctcaacctc gatccccacg tcgtcatcgg caaggcgcgg agcctcgacc
    12961 tgatcggcgg cggccgcacc gagctcgagc aggatctggt gcgggtggcg ggaaagcatg
    13021 gcctgcgcgt cgacgaggag cgctcgcccg aagccggctg gtatttccgc tccgaccatt
    13081 tcgccttcgc caaaaagggc gtgcccgcgc tcgcctatcg cgccgggcgc gacctggtga
    13141 agggcggcat ggctgcgggc aatgccgcga tcgaggatta taacgccaat cgctaccacg
    13201 cgaccaccga cgagtatgat cccgcctggg acatggccgg cgccgagcag gaggggcagg
    13261 tcgcctacaa gcttggcctc gaactcgcca acagccgccg ctggccgggc tggaacgagg
    13321 gggtggaata taagccgatc cgcgaagcca gcgcggccgc gcggaagtag ggacccgtca
    13381 tcccagcgaa agctgggatc tcacttcttc tcttcggcgc ccgtggaagg aaggaaggaa
    13441 ggaaggaagg aaggaaggaa ggaaggaagg gagggaggga gattccagct tgcgctggaa
    13501 tgacgggggg cttgcgctgg aatggcggcc ctcagaatcc cgagaggaag ctcgcgacat
    13561 tgtcccccag cgcgtcgacc gcatagccgc cctccatcac gaccagcgtc ggcaggccga
    13621 gcccggcgat gcggcgcgcg atagccgcat aatcggccgt ctggagatcg aagaagctga
    13681 tcgggtcgcc ggcaaaagtg tcggctccgt aggagcagat caggaggccg ggcgcatagc
    13741 ccgcgatccg ctccagcgcc gtatccagcg cgcggtcgaa gcccgccagc gccgtgccgc
    13801 gcggcagcgg caggttgagc gtcgcgccct cgccgtcgcc gagcccggcc tcgtcggcat
    13861 ggccccagaa gaagggataa tcgaacttcg gatcggcatg gatcgagacg aacagcacgt
    13921 cgccgcgctc gtagaagatg tcctgggtgc cgttgccgtg atgatagtcg acgtcgagga
    13981 tggccacccg tttgcggccg gccgcgagcc cggcttcggc ggcgatggcg gcgttgttga
    14041 gatagcaata gccgcccata taatcggcgc cggcatggtg gccgggcggc cggcacaagg
    14101 cgaaggccgc gcgctcgccg ccgagcaccg cgtcaagcgc cgacaaggcg gtctgcgcat
    14161 tccagtaagc cgattcccat gtccccgcgg cgatcggggt ggaggcgtcg tagctatatt
    14221 gcccgagcag cgcgtcgatc cgcgccagcg ccagcggccg ccgaccgacc accggccaga
    14281 catagccgtg cgcgtcgccc ggccgccccg cctgcagcca ttcgccatga gcccgcttca
    14341 ggaagtcgac ataatcctgc gggtggacgc ggaggatcgg ggcctcgcca tgatcggccg
    14401 gcgcttcggt cgctccgacg gcggcgagca tcgcctccac ccggcccgga tgctcggcat
    14461 aagcggtata gccgccattg tgcagctctt ggctgggggc gtgcgccagc tggcgcggat
    14521 cgaaaaacgt gcgcaccggc ggcctactgc ccgcgcagat ccgacggcag ggtaggctcg
    14581 gagatcaccc gctccatcgc cgcccagcgc gccttcggcg tcggcccggc cgcctcgaca
    14641 tgcgcgcgga ccatgtcctg gccgagcccg taattgatca catagctccg atattggtcg
    14701 acgaagtcca tcgactgctc ggcgcgcttt ttcgagacga gctggtattt ctgggtgagc
    14761 tcgaccgcct cggcgcggtc gatccttccc tccagataat cggcggcgat ggtgaagcgg
    14821 gcgccggcga ggtccttcac cgcttcgagc agccccagat aacgcccggc atcgcgggtc
    14881 ggcaggcccg ccagcgggta gagcgcctgc gtctcgtaga gcagcctctc cgcgcccgga
    14941 aaggcgaggt cgatgccgta attggccgaa ccttcggcga tcagcgactg cggcgaataa
    15001 agcgggtaca cgctatattc gacccagccg cgctcccgca tcagcctccg ctcgagcagc
    15061 atgttgagcg cgtgatgccc gggatagcct tcgtggcagc cgagatcgac cgcgcggctg
    15121 atccggatcg gcaggtcggt attgatctgg atgaggctgc tgagcttgcc cttgtaccaa
    15181 ttatagcccg accagctttt gccggtgacg aactccatcg tgaacgtctc gccctcgggg
    15241 agcgcgatat tggcggcggt gcggcgcttg cattcggcga tggcggtgtc gaacaccggc
    15301 ttcagccgct ccttggggat ggtgaagtgc tcctggaagg cgtccacccg ctgccacagc
    15361 gggccgctac ccggcgccag ccgctcgatc cgcgccaaca ccggatcgta gctcgccagc
    15421 ggcttcagct cggggcggac gccgaacagc ccctcggctt cgtcctggaa gctcagtttc
    15481 tcgccacgca gcatcctcag ccgggtgtgg gcggccttga gctgcgcgag caggaaagcg
    15541 cggcggcgct gctccagcgg gccgagcccg gcggccggca ccgcgttgac ccggctggtg
    15601 agcgtgacgg cctcggtcgc caggtcctgg agcgagcgcg gcgccgcctt ggcggcatcc
    15661 tgccattcgg ctgggccgta ataagcgtcg acatagccgg gctcgcgctc gcctatctcc
    15721 agcgtcatct ggacataatc ggcggcgatc tcgtcgaggc tgtcgtccgc ggtgcggtcg
    15781 gagacgcagc ctgccaggag cagcagccaa gccgccgcga gggagagcaa tttcatgccc
    15841 gcttttcttc aagcgaaacc acgtcgccgt caacgtcgat ctcgccgcct tgctggctct
    15901 ccgcttcctc gaagcggcaa tgctgcggcg ccagccgcag gaccgcatag ccgaccagtg
    15961 ccgacagcag cgagccgagc aggatgccca gcttcgcttc ctcgaccagc tcttctcgcc
    16021 ccggaaaagc gagcgcgccg atgaacaggc tcatcgtgaa gccgatgccg cagagcaggg
    16081 acacgccgta aatctgaagc caggtggcgc cgcgcagccg cgtggcgaaa ccgagccgca
    16141 ccgccagcca gacggagccg aaaattccta cctgcttgcc gacgaacagg ccggcggcga
    16201 tgccgagcgg cagcggcgcg aacagttggt cgaggccgac atcgtccagc ggcacgccgg
    16261 cattggcgaa gccgaacagc ggcacgatca ggaacgcgac cacgggcgtg atcgcatgtt
    16321 cgagccggtg gagcggcgat tccggggcgt ccggagcgcc cggagtcttg atcaccggga
    16381 tcatcaccgc cgccagcacg cccgcaatgg tggcatggac cccggacagc aaaaccgcat
    16441 accagagccc cagcgccagc agcatgtaga gccaaagcgc ggtgacgccg cttttgttga
    16501 ggaagtacat gacgagcagg atcgcggccg cgatgccgag cggcagcgtg gcgatcgagg
    16561 cggtgtaggc gagcgcgatg atcgccaccg cgcccatgtc gtcgacgatc gcgacggtgg
    16621 tgaggaacag cttcagcgag gtcggcgcgc gcttgccgag cagggccagg actccgatcg
    16681 cgaaggcgat gtcggtggcg gccgggatcg cccagccgct ggtgagcgcc gggtccggcg
    16741 cgacgatcaa caggtagaag ccggcgggaa ccaccatccc cgcggccgcg gcgaggaacg
    16801 gcagccgccg ctggctccag gtcgacagcc gcccgtcgac gaactcgcgc ttgatctcca
    16861 gcccgacgag caggaagaag atcgccatca ggccgtcgtt gatccacagg tggatcgtca
    16921 tcggcccgag cttgggcgtg agcgccgggc tgaccggatc gtgcagcgca tgatggtaga
    16981 ggtccgccag cggcccgttg gcgacgatca gggccgccgc cgccgctccc atcagcagga
    17041 ttccgcccgc cgcctcgctc ttgagaaatt ggcgtaacgc tgatcgcccg ccggtcggct
    17101 ttctcgcctc ggccattccg tctccttccg ggttggcagt cccctaaccg ctggatggcg
    17161 gggagggaat ggcttaagcc cgccatctat tgggggctgc agccgcacgc cgatgaacga
    17221 attgaagcca gcgacgatca cggcctcccg ccaccccttc ctgcaatggt ggtcggacca
    17281 tgtcatggcc tcggtcgacc accgcgaggt ggtcgagaag gtccaggagg atgccggctg
    17341 gtcctcgcac tatgtgttca tgacgttgat gtcggccggc atcgccgtgc tcggcctgct
    17401 gctctcctcg cccgccgtgg tgatcggggc gatgctgatc tcgccgctga tgggcccgat
    17461 catcggcctg ggtttcgccc tcgccacctt cgattcggcg gagatgaagc gctccgccgc
    17521 ggcgctcgcg ctcggcgtgg cgctggcggt gttcttctgc gccttcgtgg tgctgttctc
    17581 gccgctccag acggttaccg acgagatcgc gtcccgcacg cgccccaatc tgttcgacct
    17641 cctcgtcgcg ctcttctccg ggctggcggg cacctatgcg atgattcgcg gccgccacgg
    17701 caccatcgtc ggcgtcgcca tcgccaccgc cttgatgccg ccgctggcgg tggtcgggtt
    17761 cggcctcgcc acggccaatt ggccggtctt ctggggcgcc tccttgctct tcttcaccaa
    17821 cctcatgacc atcgccgtgt cggcggcgat actggcgcgg atcttcggct tcgccgccaa
    17881 tctttcgccc cggcagaccg ggctgcaggc gactttggtc gtcgccatct tcgtcgcgct
    17941 cggcgttccg ctcggcctct ccctgaagca gatcgcctgg gagacgctcg cctcgcgcca
    18001 ggcccgcgaa gtggtggccg gccatttcgg cgaggagggc cggctcagcg agatcagcgt
    18061 cgattacgac gcccggccga tcgcggtcga agcgaccgtc ctcacgccgc gccatcgccc
    18121 cggcgccgag cgcgagaccg cggcatcgct ggagaagatg ctcaaggtcc cggtcaaggt
    18181 caccatcgac cagttccggg ttgggtccga aggcgaagcc gaggccgccg cgctcgccgc
    18241 cgcccgcggc agcgccgccg agcgcgcgcg gtcgcgcctc gccgagcggc tggcgctggt
    18301 ggcgggcgtc tcccccgacg aggtgctggt cgaccgcgcc aacagccgcg cgcaggtccg
    18361 cgccgccgcc ttgccgggcg cggacctcgc cagctatcga gcgctggagg cgcgcgtggc
    18421 ggcgtccgaa aggggctgga ccatcgcgct ggtcccgccg gccgcgccgc tgcccgcgat
    18481 tgccttcgag gatggacagc cggacggcgc cgcgctcgcg accgccattt gggccgcgcg
    18541 gcggctcaac ctgccggtcg gggtgagcgg cggcagcgac acgcaggccg aagcagtgat
    18601 cacggctctc cgggaagccg gcgtggacgc gcgccgcagc gagggcggcg gcgggggggt
    18661 ggagttgcgc tggctcgcgc cgcgtgtcgc ggccgaggaa taaaaggcca agcacaccgt
    18721 atttgccgca gattgcgcgc gatttctcca aaacggatta tgacgtctcc gaatcggaga
    18781 gattctgtgg tggggctcga gccgcttttt gccggcctcg acttcgtcat gcgcgaggcc
    18841 gcgctgttcg cggccaccag ctttcttgtt ctcggcgccg gcgacgtcct ggtcgacctc
    18901 ctctggctgg cgctggtggt gcggcggggc gtggccggcg accgcctgat ccgcgccagc
    18961 gatctcgccg gcgcagggca gcccggcacc atcgcgatct tcgttcccgc ctgggatgag
    19021 gccgaagtga tcgagccgat gctgcggcac gccttgctgc ggctccggca cgaggatttc
    19081 gtgatctatg tcggctgcta ccccaacgat cccgccacca tcgcagcggt cgcgagggta
    19141 ggcgatccgc gcgtgcggct ggtggtcggg ccgctgccgg ggccgaccac caaggccgat
    19201 tgcctcaacc gcatctgggc ggcgatggtc ctcgacgaga ggctgaagcg gcggcgggtg
    19261 cgggcaatcg tcctgcacga cgccgaggat gtcgtcgacc cggacgaatt gttcgtcttc
    19321 gacgtgctga tcggccgctt cgacatggtc cagctcccgg tctttccgct ggtcgacgag
    19381 aattcgcgct ggatcgccgg ccattatgcc gacgagttcg ccgagtcgca cggcaaggag
    19441 atggtggtgc gccaggcgat cggcgccgcc ttgccgtcgg ccggggtcgg ctgcgccttt
    19501 gcccgcgagg cgctggagga cattgccgcc gcgcggggcg gagggccgtt cgacgcgggc
    19561 agcatcaccg aggattatga gctcgggctc aggctgcgcg ccttgggcgg gcgcgccgcc
    19621 ttcgtgaggc tgcccggccg cgacggccgc tcattggtcg tgaccaagga atatttcccc
    19681 gccacgctcg aagccgcggt cgcccagaag gcgcgctgga tgaccggcat cgccctcgcc
    19741 ggttgggacc ggctcggctg gtccggaagc ctcgccgagc gctggatgag gatccgcgac
    19801 cggcaatcgc tgctcgcggc gctggcgctc ggcgccggct atgtcgccgg cctcatctgg
    19861 acaatgctga cgctgcgcca gatcttcctc ggcatcgatg ccgacccggt ggcgccgctg
    19921 ctcgctctat tcctgcagat caacctggcg atgctcggct ggcgcctgat ggtgcggttc
    19981 gccttcgtca cccatgccta tggctggcgc gaggggctgc gctcgctcgc ccggattttc
    20041 gtcggcaata tcatcgccat gctggccgcc cgccgggcga tcctcggcta catcgccatg
    20101 cggcgcaccg gcagcgcgcg ctgggacaag acggcgcatg ccttcccggt tgaaatgccg
    20161 gccgaatgag ggccgctccg ctacgcttcc tggtggtggt ggcgggcggc tggatttgcg
    20221 cccgcgcggc gatcctgatg ccgtgggcgg agagcgcggc cgagcccgcg cgccaatcgg
    20281 cggctggtgc ggacttgccg ttcgccgccg ggcctccggc gcccggtccg ggccgcgcgg
    20341 cggagcgggg ctccaacctg gccggggcca cagtcatttg gccgacatcg ctgcatcgcc
    20401 cgaggcaatt tgacttggtg cggagttcgt cggcgaccgg agacgcgcct gatccgctcg
    20461 atccagtcac caccttcgcc gccactttcg gcgccgccga tactttgccg gcaccggctc
    20521 ccgcctcgct tccggtgcag gcggccgcgc cgctggcggt gccgcctttc gccccggatc
    20581 ggccgcccgg ccgctggtcg gcttcgggct gggccttcct ccgccgcggc ggtgagcctt
    20641 cgctggcctc cggcggcacc ctcggcggca gccagatcgg cgcccgcatc ttgtaccgcc
    20701 ttaaccgcga cgccgcccgc ccgctgtcgc tggcggcgcg cttctattcg ccgctggaaa
    20761 gccgcggcgc cgaggcgtcg ctgggggtcg agtggcagcc gctggccggc gtgccgctgc
    20821 ggttgctggc cgaacggcgg caggcgctgg acgatcgggg ccggtccgct ttctcgctgc
    20881 tcgcgcatgg cggcgtcagc gatcgccgcg tcgtcgggcc gctgctgctc gacgcttatg
    20941 cccaggccgg catcgtcggc ctgcgctccg ccgacttgtt cgcggatgga tcggcgcaga
    21001 tcggcatccc tatcgggcgg gaagggcggc tcaaggtcgg cgccggcctg tggggtgcgg
    21061 tccagcccgg cctgtccagg ctcgatgccg gtccgcaggc ctcataccgg atgcggctgg
    21121 gcggcgccgc cgtccgcgtc gccgccgaat ggcgcttccg gatggcgggg gacgccgccc
    21181 cggcctccgg cccggcactg accgtcgcca ccgaatttta gcgggccgcc ttccttgccc
    21241 cgccggaagc cgctagcgct tgccccgtgg acatctacct tccgatcgca gggctttccg
    21301 tcaacgcttt ggtgatcatc ggcctcggcg gcgtggtcgg gctgctgacc gggatgctcg
    21361 gcgtcggcgg cggcttcctg accacgccga tcctgatctt ctacggcatc ccgcccgccg
    21421 tcgcggtcgc ctccgccacc acccagatca ccggcaccag cgtctccggc ctgctcgcgc
    21481 accgccggcg ccggggcgtg gattaccgga tgggcggtgt gctggttgcc ggcggaatac
    21541 tcggcgcctt ggccggcggc ggcctgttcc ggctgctcca ggacagcggc caaatcgata
    21601 ccgtgatttc gcttctctac gtcatcctgc tcggcagcat cggcgtgctg atggcgaagg
    21661 aatcggcgac cgcgctcgac atcatcaagc cgaccgcgcg cgccaagaag aaagcggcgc
    21721 ggcggcacaa cccgctgatc gcggctttgc cgatgcgctg gcgcttctat cagtccgggc
    21781 tctacatctc gccgctggcg ccgctcgctt tgggcttcgt gtcggggatg ctgaccgtgc
    21841 tgctcggcgt cggcggcggc ttcatcgtcg tccccgcgat gatctatctg ctcggcatgt
    21901 cgaccatggc ggtcgtgggc acatccctgc tccagatcct gttcgtcacc gcggccacca
    21961 ccctcgtcca tgcgaccacc accaagtcgg tcgatatcgt gctcgccgtg ctgctgctcg
    22021 tcggcagcgt gatcggggcc cagatcgggg tgcggctcgc gcagaaggcg aagccggagc
    22081 tgctccgcat gttccttgcc atcgtcgtcc tggcggtggc gctgcggatg gcgctccagc
    22141 tcggctggcg ccctgccgaa atctacacgg tccagtcgct gtgaggcggc tgcgcgcctt
    22201 gttgctcgcg ctcgcgctgc cgctcacgct cggcgcgacc gagccgctgc tcgtgcccga
    22261 cgtgtcgcag cgcaagatcg agatccgcta ttccttcacc ggggcggagc tgctgctgtt
    22321 cggcgcgatc atctatccgg gcggacgcac ccccaaggag ccggccgaca tcgccgtggt
    22381 ggtcaagggt ccggccgagc cgatcctggt cagggagaag cagaaagttg ccggcatctg
    22441 gatgaacgtc gaaagcgagc gcttccgctc ggccccctcc ttctacgcgg tcgcctcctc
    22501 gcgcccgctg accgagctgg tcgacgagcg gatggccgcg atctacgagc tcgggctcga
    22561 aaatctgcag ctctcgccgg gcgccggcgc cagccccgag gagcgccggc ggttcgagag
    22621 cggcctgatc gagctgatgc gctcgcacca gctctacgtc gaagcgccgc gcggcgtcga
    22681 gatcaccggc ggcgtcctct accgcgcccg cctcaatatt ccggcccggg tcccggtcgg
    22741 caattatacg gcggagacct tcctcatcca ggacggccgc gtgctggccg cggcggtgcg
    22801 cgagatcagg atcgagaagc tcggcttcga acgcttcgtc gccgaggccg ccgagcgctg
    22861 gtcgttcggc tacgggctgg tggcggtgct ggtttcgctg ttcctcggct ggggcggaag
    22921 cgcggttttc cgccgcgtgt gaacccccgc ggggccacgc acatcactta tttacccttc
    22981 ttcgttagca aaacgcggtg gaacgcaacg caaatgaaag tccgcccgtc accatgaccg
    23041 acatgatgaa ctatcaaagc tttcaggatg acgagcaggg cgagacccgc cgccccacca
    23101 tcaagcaggt cgaattgccg cctccgacca cccggcacga gcagatcggg aagatcgttc
    23161 aggtttccgg cggcggcgcc cgcatccaga tgcggagcgc gcggctgcgc gagctggcgc
    23221 tcgagaccga tcccagcgtc gccatgtccg gccaggtcgg cagccaggtg aagctcgaat
    23281 ccggccggcg ctggctgctc gccaacgttc gaaacctgtg cgtcaccgac agcgagggcg
    23341 acgtcatcgt cgccgagatc gactttcttg gcgagggcga cgaggacgag cagaccgggc
    23401 gcctggtcaa cttcaagcgc ggcgtcaccg gctacccgat cccgggcaat cgcgtgttcc
    23461 ccgtcaccag cgccgatttg aagcagatgt tcgccgccga cgagcggccg catatcgaga
    23521 tcggcaccgt ctatccgacc aaggacgtgc gcggcgcgct ctatgtcgac gcgatgctcg
    23581 gcaagcattt cgccctcctg ggctcgaccg gcaccggcaa gtccacctcc gcggccctga
    23641 tcatgcaccg catctgcgac atggcccccg aaggccacat cgtcatgatc gatccgcacg
    23701 gcgaatattc ggccgccttc aagggctatg gcgagctgtt caacgtcgac aacctcgcca
    23761 tgccttattg gctgatgaac ttcgaagagc attgcgaggt gatcgtcacc tcctcgggcg
    23821 ccgaccggca gcgcgatgcc gacatcctcg ccaaatgcct gctccacgcg cggtcgaaga
    23881 accgggtggc ggaggggctg accaagctca ccgtcgattc gccgatcccc tacacgctca
    23941 ccgatctcac caccgcgatc accaacgaga tgggcctgct caacaaggcg accgacaccg
    24001 cgccctatat gcggctcaag accaagcttg acgagctcaa ggccgatccg cgctacagct
    24061 tcatgttctc cggcatgctc gtcgccgaca gtatgaccag cttcatctcc aagatcttcc
    24121 gcctgcccgc caagggcaag ccgatctcga ttgtcgatgt gtcgggcgtc ccgtcggaca
    24181 tcacctcggt ggtggtggcg gtgctgtcgc ggctggtgtt cgattatgcg atctggtcgc
    24241 gcaacgagct ccagcgcccg gtcctgctca tctgcgagga agcgcatcgc tacgtgccct
    24301 ccgacaagac gtcgagcggc caggccgtgc gcaagatcct cgagcggatc gccaaggaag
    24361 gccgtaaata cggcgtctcg ctcggcctca ttacccagcg gccgtccgat ctcgccgagg
    24421 gcgtgctctc gcaatgcggc acgatcatcg cgatgcggct caacaacgag cgcgaccagg
    24481 ccttcgtcaa atcggcgatg ccggagggcg cgcgcggttt cctcgacgtg atcccggcgc
    24541 ttcgcaaccg cgaatgcatc gtctgcggcg agggcgtgtc gatcccgatc cgggtctcgt
    24601 tcgacgatct cgagcgcgac cgccggccgg cctcgtccga tccggccttt tccgagctct
    24661 ggaagcagac cggcgacgag gaggcgatgg tcgcccgcgt cgtcaaacgc tggcgcgccc
    24721 aggggcgcta agcctcaggg gggagcaggc tccctgccgt catcccagcg gaagctgggt
    24781 tctcactatc ttcacaccaa cttcacacca gacaagggaa cgtcatccca gcgaaagcgg
    24841 ggatctcact gcctttgatc tcgaagagag attccagcgt gcgctggaat gacgatccgg
    24901 cgcgaaagtc gcctcaggtc cgcccggcgg ccttgcccgg ctccgccttc gcctgctgga
    24961 cctcgccgcg cacggcgccg acgggcaccg acagcagccc gtgcaggcgg ctgcggaagc
    25021 cggccagctc ctcgccgctc agctgcgccc gcatggtgaa tttcaccgac gccggattga
    25081 tcggaacgcc gttgcgataa agctcgtaat ggaggtgcgg cccggtcgag aggccggtcg
    25141 aaccgacata gccgatcacc tggccctgct tgacgaacgc gcctggctgg gcggtgatcc
    25201 ggctcatgtg cgaataggag gtcaagaggc cgccgccatg gcggatgcgg acctggcggc
    25261 catagccacc ggcccagccc gccgcggaaa tgcggccctc ggtggcggcg aggattgggg
    25321 tgccgtaacc cgccctgaag tccatcccct tgtgcatccg cgaatagccg aggatcgggt
    25381 ggcggcgcat gccgaagttc gaggagaccg cgccgggaac cggccgctgc agcatgccgc
    25441 tcgccttgcc gacgccggtc gcttcgaacc attgcgtctt gccgccctgc tcccactgca
    25501 tcagctggag gtccttgccg cccgagcggc ggaggcccgc gaacaggagc ttgccggtct
    25561 cggtctcgcc ggtcgcggcg cggcgatgtt cgaggatgat gtcgtagcgg tcgtccgcct
    25621 cgacgctgcc gatgttgatc tgcccggcaa tcgcccggat ataggcttcg accgccttgg
    25681 ccggcgcgcc ggcggcgcgg gccgatcggt acaggctcga tccgaccaat ccctggatgc
    25741 ggagcggcct attgtcgacc gcgatcggga tcggcgtcat ggccagccgg ccgtcgaccc
    25801 gggcgatctc cagcttgagg tcgacgcggg cgcggaacgc cagcgcttcg agcggccggg
    25861 cgacgctctt gttcggccgg cgcccgagcg tgatgtcgag catggtgccg ggccggatct
    25921 cgtccagcgg gaccgccccg gccaccatcg cgccgatcgc gtcggcttcg gcgcgggcga
    25981 cgccggcgcg ttcgagcacg cgcgtgaagc cgtcgccgcg gccgagcgtg gcgaacaggt
    26041 caacgctcgg tctttccggc gtatcgagca aaggcgcgac cgcgtcggtc ggcgacatcc
    26101 ggcggccggt gtcggcgcca agcgccagcg gcgagatggc gagcgcgcgc gcttcctccc
    26161 attgcgcctc cggaagcggg gccggcgatg cgcccacaag cgcgctcgcg ctgggagcga
    26221 acgaccaggc gccgtagcaa agcgcaaggc aggtagcggc gccgcgcagc cattcgcgcg
    26281 atccgatcct gacaccgagg tcgacgacca ggtcgagttc gccgatccgc gcgcgcaggc
    26341 cgtggagccg attatgggat gtcggagcgg ggcgcgcgcg aagcagtgcg gcatcgaggc
    26401 tgagggcagc cgtgcccccg cccgcgccgg cgctgaaatc gttgaactga tacaagcgga
    26461 aaagcccccg ctgcccctgg aaaaccccgc tctcgcgtgg cccgatgact gtggggaaag
    26521 agggtaaaag taaagtaaag cgaatccggc cttcgttcca gttgcgacga gccgcctgca
    26581 tggggcggcg ggacgagccg cggcgatgga accgctttga tttgttgcgc ttcctccttg
    26641 ccgtgccaat ctagcgggca tgagcgattt cgcgcgcgcg ccgctggccg ccatccttgg
    26701 gccgaccaac accggcaaga cccacctcgc ggtcgagcgg atggccgcgc attcgagcgg
    26761 catgatcggc tttccgctgc gcctgctcgc acgcgaagtc tacgaccgcg tcgtcaagct
    26821 caaaggcccg aagcaggtcg cgctcatcac cggcgaggag aagatcctgc cgaaggacgc
    26881 gcgctggttc ctgtgcaccg ccgaatcgat gccgatggag cgcgacttcg cgttcgtcgc
    26941 cctggacgag gcgcagctcg gcgccgatcc ggagcgcggc cacgtcttca ccgatcgcat
    27001 gctccacgcc cgcggccgcg aggagacgat gatcctcggc tcggaggcgc tcgcgccgat
    27061 ggtgcgcgcc ttgctgccca aggccgagat cattggccgg ccgcgtttct ccaccctgag
    27121 ctatgccggc gcgaccaagc tgtcgcggct cccgccccgc tccgccatcg tcgctttttc
    27181 ggccgagcag gtttatgccg tggccgagat gctgcgccgg atgcgcggcg gcgccgccgt
    27241 cgtgatgggc gcgctctccc cccgcacccg caacgcccag gtggcgatgt accaggccgg
    27301 cgaggtcgat tatctcgtcg cgaccgacgc gatcggcatg gggctcaaca tggacgtcgc
    27361 ccatgtcgcc tttgcgggcc tgtcgaaatt cgacggccgg cgccggcgcc gcctgacacc
    27421 cgccgaaatg gcgcagattg ccggccgcgc gggacgccac cagcgcgacg ggacgttcgg
    27481 gtcgctgacg ctggagggcg agtcggccgc cgaattccgt cccgaggaga tcgacgcgat
    27541 cgaagggcat cgcttcgccc cgctcgacca tcttttctgg cgcgagggcg atccggattt
    27601 ccgcagcgtc gacgcgttga tccgttcgct cgagcgccgg cccgaccggg cgtgcctcag
    27661 ggcggcgccg gaggcggtcg acctcgccgt gctgaagcgg ctggccgagg atccggaggt
    27721 ccgcgcccgt gcgaccggtt acgaccgggt ccgcaccttg tggtcggctt gcgggctgcc
    27781 cgatttccgc aagaccggtc ccgagcacca tgcgcgcctg gtcgcgcgcc tctacaccca
    27841 tctcagcgag ggcaatggcc gcattccgca gggctggtat gccgagcagg tcgcccggct
    27901 cgacaatgtc caaggcgaca tcgagacgct tgccgaccgc atcgccggcg tgcgcacctg
    27961 ggcctatatc gcccatcggg ccgactggct ggtgagcccg gaggagatgg cggagcgcac
    28021 ccgcgcggtc gaggagaaat tgtccgacgc cctccacgag cggttgaccc agcgcttcgt
    28081 cgaccggcgc acgtcggtgc tgctgcgcga catcggccgc aagggcgcgg gcgaattccc
    28141 ggtgctggtc gacgagcagg gcgaggtcac cgtcggcagc ttcccgatcg gccggatgga
    28201 gggctttgcg ttcgaagtcg atccgaccgc gcgccacgcc gaccgcaaga tgctgctcgc
    28261 caccgccgag cgccggctcg ggggggaata tgagaagcgc gccgccgccc tggtcgccga
    28321 cacggacgac catttcacgc ttcggaccga gccggggcag ccggtcgcga tcctgtggcg
    28381 cgggcatgaa gtggcccggc tcgggcccgg caagaatctg ctgtcgccgc gggtgctgct
    28441 cgaccgccgc atcgagcggc tgtcggaacg cggccgcgag gcggtgatcg agcggctcaa
    28501 aaattgggtg cggcaccagg tcgagcgcgc gctcggcccg ctccgcgcgg ccggggcggc
    28561 cgcccaggat gccgccgcgc cgtccgcggt ccgcgcgatc gtcgcgatgc tggtcgacga
    28621 tggcggcatc gtcgcgcgcg atgccgtcgc ccagccgctc gctcagctcg atcgcgagca
    28681 gcgccgccac atcgcccggc tcaaggtgag gatcgggacg ctcgacctgt tcatgcccga
    28741 tgtgctgaag cccgaggcgc ggcgctggcg gaccgcgctt cgcgccgccg cgtcgggcgc
    28801 ggccatgccc gagctgccgc cgcacgccag cgtggtcctg ccgacgcccg aggacgagcg
    28861 gcgccttctc ctcgcgcggc tcggcttccg ggccctcggc ccgcagatgc tccgcgtcga
    28921 cctcgtcgag cggctcgccc gccacgccca tgaggcgcgg gcgggcaagc atggccaggt
    28981 ggtcgacgag acgctcgcca cctcgctcgg cctgcagccg caggcgatcg cccggctgat
    29041 gaaggagatc ggcttccgtc cggcccagag cgaggccggc tggatctgga caggacgcga
    29101 gcgccgccgc cccgccaccg ccgccgggcg gggccatgcc ttcgccgcgc ttgccgggct
    29161 ccgccggggt ggctgacagc ctgcggctcg acaaattcct gtggttcgcc cggatcgtaa
    29221 agacgcgcgc tttggcccag cagctcgccg agcagggccg gctgcggctc ggcggccggg
    29281 tcatcgaccg cgcccatgct ccggtccggg tcggcgacgt gctgagcttc gccgtccgcg
    29341 gcaacgttcg ggtgcttcgg gtggaagccc ttccaacccg tcgcggcccg cccgccgagg
    29401 cgcgggcgct ttacagcgaa gtcgtgccgg ccgacgggac gttgacgagc cgaggcgcgg
    29461 gcgactaggg aatccccgtc caggagaagc gtcaatgacc tatgtcgtca ccgaagcctg
    29521 tatccgctgc aaatatatgg attgcgtgga agtctgcccg gtcgactgct tctacgaagg
    29581 cgacaacatg ctggtgatta atcccagcga atgcatcgat tgcggcgtgt gcgagccgga
    29641 atgcccggcc gaggcaatcc tgccggatac cgaggtcggc ctcgaaaagt ggctcgaggt
    29701 caacgcgacc tactccgccg aatggccgaa catcacccgc aagcgggaat ctccggccga
    29761 cgccgacgag cataagggcg aggcgggcaa gttcgacaaa tatttcaagc cggaccccgg
    29821 ccaaggcgat taggctcggg cgttgcggcg gaggcggcgc ctccgtcaac cggcgcttcg
    29881 acaggcggcg gcagatttcc gatgaaggga gtcgaaaccc tagcattttc gcctcattta
    29941 tgttacatag tcgtcatcga agcgggtgct gtcgcatccg cgttttggtg atctagaaga
    30001 atcgccgcaa aattctgtga ctgggactgc gtcttgaacg gggcggaggc agcttggcac
    30061 gttttgcggc tatttccacg ggaaggttga ttcatggcag cgaaggcgtt gaacttcgac
    30121 gttggcgatt atgtggtgta ccccaagcac ggcgtgggcc gtgtcatcga actgcagagc
    30181 acggatatcg ccggcatgca gctcgaactg tacgtgctcc ggtttgaaaa ggagcggatg
    30241 acgctccgcg tcccgaccaa caaggccgag agcgtcggca tgcgcaagct gtcgtccgac
    30301 aagacgatgc aggaggcgct cgccaccctc aagggcaagc ccaaggtgaa gcgcaccatg
    30361 tggtcgcgcc gcgcccagga atatgaggcg aagatcaatt cgggcgacct ggtgtcgatc
    30421 gccgaagtga ctcgcgacct gttccgcgcc gacgaccagc ccgagcagag ctattcggag
    30481 cggcagattt tcgaggcggc ctcgagccgg ctcgcgcgcg agctcgccgc gatggaggag
    30541 gtcgacgagc ccaaggcgct cgagaagatc ctcgacatcc tcaacaaggc ggccgccatc
    30601 tacaacaagg aaaaagcggt cgagaaatga tcggcctgga tgggtggcgg ccccgcatcc
    30661 ggggcatgac gccccccatc cgcacagcat ccgaagatga aggagaggcg gcccggcgac
    30721 gggccgcctt ttttcatgtt gcggttgggc ggtccgccgt gtattgtgta actaacacag
    30781 tcaaggagaa tgccatgcgc tccgcgctca tcctggtctc gacgcttgcg ctcgccgcct
    30841 gcaacatggc cggcgccgaa gagggcgaag accggtccgc cgcccccgcc gcgcggcaga
    30901 gctacaatgt cggcagcttc gattcggtct cgctcggcgg tccctatgac gtcgtcgtcc
    30961 aggtcggccc cgccgcctcc gtgcgggccg agggcgatgc cgaggagatc gcgcaaatgg
    31021 tggttgaggt gaagaatggc agcctcgaaa tccgcagcgc caagcgcagc ggcaatttca
    31081 cgatcggctt cagccgcgac cgcaacgtca ccgtctacgt caccgccccc tcgctccgcg
    31141 ccgccgcgat cggaggttcg ggcgacatgc gcatcgacaa agtggagggc ggcgagttcg
    31201 ccgcttcgat cggcggctcg ggcgacatga cgatcgccgc gatgcgggtc ggcgaagcga
    31261 gtttctcggt cgccggctcg ggcggcatcc gcgccgccgg caccgccgag cgctcgagcg
    31321 tctcgatcgc cggctccggc gacgtgatcg tggacggtct gaagagccgc tccgcgtcgg
    31381 tctcggtcgt cggctccgga gacgtccagg cgaacgccgc cgagaccgcg gacgtctcga
    31441 tcatgggctc gggcaacgtc aagatcgcgg gcggcgcgcg ctgctccgtc aacaaggtcg
    31501 gttccggcga cgtcagctgc ggctgacgcg cttggttatc agcccttaac cattggacgc
    31561 catgatcctg gcttatgaac aggatcatgc tttccgccgc cgctctcgcg cttgccagcc
    31621 tcgcggtcgc cgtccccgcg gccgccgccg aacggcgctt caccgtcacc cagttcgacc
    31681 ggatccaggt cgacgggccg ttcgcggtca ccgtcaccac cggccgtgcg accggcgcgc
    31741 tcgccaccgg cgatgcgcgc gcgatcgacc gggtctcgat cgaagtgcag ggccgcgtcc
    31801 tcaaggtgcg cccgaaccgg tccgcatggg gcggctatcc cggcgagggc gccggcgcgg
    31861 tcgcgatcgc agtctccacc catgaactgc gcggcgtcgc ggtaaacggc tccggcaccg
    31921 tcgcgatcga caaggcgcgc gcgatgcggt tcgacctctc cgtctccggc agcggcgtcg
    31981 tcgagcttcc gaacctcgag gccgaccggc tcgtgctcgg cctgctcggc tcgggccgca
    32041 tcacgctcgg cggcaaggcc aagacggtgc gggcgacgat ccagggcacc ggcgacctcg
    32101 ccgccgccga gctgatcgcc gaggatgccg agctcaacgc cgacagctcg ggcaagatcg
    32161 cgcttggcgt caagcgcgcc gtcaaggtga cggcgaccgg cgccggcgat gtcgagattg
    32221 ccggctcgcc cgcctgcacc gtcaaggcgc tcggctccgg ccgggtcagc tgcggcgccg
    32281 actaggccgt tagttgcccg ccacgaatct tagatcagcg ccagcgccga aagcttgatc
    32341 gcgagccccg gcggcatcgc ggcgatgccg aggtcgctgc cgtcgaaatc cagcggcgcg
    32401 tcatcggcgg cgaggtagcg ccagccctgg tgcgcgcgct tgggccaggc cctgaccggc
    32461 accagctccg ggtcgagctg gatcaaggtg cgccgctcct ccgcctcggc aaagccgacg
    32521 atccgattgc gcgccgtgat ccggtgcttc acgatccaga agatcgagcc gccgatcagc
    32581 tcctcggccc gcttcggccg gaaccgcgtc gtgatcggca cgatgccagc gctcagccgc
    32641 gattgctggc gcttgcggag cgcgtcgaag ctggcgcagc cgaccgcgac cttgctgata
    32701 tggagggagg acatcgcccc agtccttgcc gcactagcgc ccgcggatca acagcttagc
    32761 cgacgaggcc ggaggccacg gcaaggccga ggaaggcgaa gaagcccatc gaatcggtga
    32821 tcatcgtcac gaacaccgac gaggcgacgg caggatcctg atcgagccgc tcgagcgcga
    32881 gcgggaccag cactccggca aggccggcaa tggcgatgtt gatcagcatc gcgacggcga
    32941 tgacgccgcc gagcgccgga ttggagaaca ccacgcccgc tccgatgccc accagaacgg
    33001 cgatggtgag gccgttgagc aaagcgagcc ggacctcgcg ccagatcgta cgcaagctgt
    33061 tcgaccgggt gagctggttc atcgccagcg cgcgcacggc gacggccatg gtctgggtcc
    33121 cggcattgcc gccgatcgag gcgacgatgg gcatcagcac ggccagcgcc accatctgct
    33181 cgatggcgcc tccgaaggcg gcgatgatcg aggaggcgac caaagcggtg aacaggttgg
    33241 cgaccagcca gcgtacccgc gcgcgatagc tgtcgcggat cggctggttg atgtcgcctt
    33301 cgtcgcccgc gcccgagagc aaaagcacgt cctcgctggc ttcctcctgg atgatgtgga
    33361 cgatgtcgtc gaccgtgatc atgccgacca gccgccccga ttgatcgacg acggccgcgg
    33421 agatgagcgc atatttctgg aagcggagcg cgacttcctc ctggtccatg tcgaccggga
    33481 tcaaggtctg ctcgcgctgc atcaggtcgg ccatggcgac gtggcgcggc gtccgcagca
    33541 cccagctcag catcacggtg ccgaccgggt gatgctgcgg gtcgacgatg tagatttccc
    33601 agaagtcggt ggtgaggtcc tcgccggcgc ggagatagtc gatcacctgg ccgacattcc
    33661 aatgttcggg caccgcaatc aggtcgcgct gcatcagccg gccggccgat tcctccggga
    33721 aggtcagcgc ctcctcgatc gcggcgcgat cgtccgggtc gagcgcgcga aggacggcgc
    33781 gctggtcgtc ctcctccatg tcctcgatga tcgcgaccgc gtcgtcggtc tcgagctcgc
    33841 ccgctatctc ggcgacctgg tgcggctcca gcgcgtcgat cagctcttcg cgcacccagt
    33901 cgttcatctc ggcgaacacg tcgccgtcga gcaggtcggc gagcgcggcg gcgagcgcgc
    33961 ggcgctcgtc gcggtcgacg atctcgaaca ggtcggcgat gtcggcgggg tggagcggct
    34021 cgaccagcgc ccgcgcgccg tccgtgtcgc ccgcttcgac ccggtccatc acctggctga
    34081 cgaattgcgg cttcagccgg tcgtcctcgt cgagccggtc ggccgcggag ggcggcgcgc
    34141 cgtcggcgat ctcgatatcg ctgtcgctca tcgccccctc tccttcgcct ggctcgcctg
    34201 ccgcctgtta cggtccgtcg cctcgcctgt cgatgtttgt gacgcggtgg gggtggcgca
    34261 tgccggacgc cgcgcctaag tcgccgcaaa gcgaatttag gagaccttca tggcgaacga
    34321 actcaacact ttggtgctca gcctcgacac cggcaacgat ggcggcggcg atgtcgtcat
    34381 ccggctgcgc ccggacctcg cccccggtca tgtcgagcgt atcaagagcc tggtccagga
    34441 cggcttttac gacggtgtcg tcttccaccg cgtcatcccc ggcttcatgg cgcagggcgg
    34501 cgatccgacc ggcaccggca ccggcggctc caaacagccc aatctcaagg ccgagttcag
    34561 cgccgagccg cacgtccgcg gcacctgctc gatggcccgc gccagcagcc ccgacagcgc
    34621 caacagccag tttttcatct gcttcgacga cgccggcttc ctcaacggac aatatacggt
    34681 ttggggccag gtcaccgaag gcatggagca tgtcgacgcg ctcccggtgg gcgagccgcc
    34741 gcgcaagccc ggccggatcg tcaaggccac gctccgcgac tgatgcgccc ggtcacgctc
    34801 gtcaccggcg cctcgagcgg tctcggcgct gaattcgcgc gccaatgctc gaggcgcggc
    34861 gacgagctgg tgctggtcgc caggcgcaag gaccggctcg aggcgctggc ggcggagatc
    34921 ggcggcaatg cgcatgtcat cgccgccgat ctcgcggccc cggaggcgcc ggaaaggctg
    34981 ctcgccgaca tcgaggcgct cggcctcacc gtcgacacct tgatcaacaa tgccggcttc
    35041 ggcctagccg gcccgttcgc caagctcgcg ctcgaccggc aggccgaaat gatcgagctc
    35101 aacgtcgctt cgctgacccg gctctgtcac ctcgttctgc cgaagatgct cggccggcgg
    35161 cagggcgcca tcctcaacgt cgcttcgacc ggcgccttcc aggccgggcc cgggatcgcc
    35221 gtctatttcg cgaccaaggc ctatgtcctc tccttcaccg aggcgttgca ccaggagctg
    35281 aagggcaggg ggatcagggt ctcggcgctg tgcccgggcc cgacccgcac cgagttcggc
    35341 gaggtcgccg gcttcaccag tgcgagcttc gaccgcttcg cggccgatgc ggccagtgtg
    35401 gtcacggccg gcctccacgg cctcgatcgc aacaaggcgg tggtgatccc cggcctcacc
    35461 aacaagctga gcgctcaatc gaaccggctg atcccccgcg ccgcgatgcg ccgcatcgtc
    35521 ggcgcgctga agctctaggg cgcggccgcg cctgccggcg ggcgcgcctc ctcgccttcc
    35581 acccattcga acagccagtc gtggaagatg cggaccggcc gccgcgccag cgcggagcgg
    35641 cggcaggcga accaatagct gtaggggctc tccaccttga cgtcgaacag ctgcacgagc
    35701 cggtcgtcat gcgcctcctt caaatggctt tcgagcatga aggcaacgcc cagcccctgc
    35761 gcggccgcgt cgaggatgag ctggcccgag tcgaaatgat cgatcgccgc cggctggagc
    35821 tcgggcaggc cgaccgcctc gcgccaatag tcgaacgtgt ccggcaggtc gcggtggagg
    35881 aagatggtcg ctccggcgag ctgggccgga tgggtcagcg cctgcgggcc gtccctgagc
    35941 tcgtgcgatc cgatcgcgat cacgcaattg ctgtcgatgc ggcgcgcata gaggttggga
    36001 tcgatctcgg tggcgatcgc gatcgccgcg tccagcccct cgtcgaggcg ggtgaggccg
    36061 tgcgcgccgg tatcgatgtc gatgtggaga tcggggtgga gcgtgcgcag ctccgacaga
    36121 tgcggcatca gccgctgcga ggcgaacagc ggcagcaccg ccagccgcag tcgcatgaaa
    36181 tcggcctcgc ccgtggcccg ctccatcgcc agcgccagcg cgtccagcga agaagcgatc
    36241 tcggccagca gccgctcccc gtcagggttg aggtgcaagg cctggtggcg ccgctcgaac
    36301 aaggggtggc cgacaaaccg ctcgaacgcc tgcagccgcc ggctcagcgc cggggaggac
    36361 agggccagcg cttccgcggc cgccttgacg gagcccagcc gcgccacctg gatgaaggct
    36421 tcgagcgccg tcaaaggggg tagcctgcgc atctggccgc cgttacggca tcctctctct
    36481 tgtgcggcgc aaagtcattg ctgcaccgca acgctcaagt aagcacgccc atttccgagt
    36541 gcaagacgtg caatcgccgg cttcctcttc gcaattgcac aatgggctga tcggggcgac
    36601 atagcctgtg cctttcaggc atcctctcct ataaactttc agggtcggtc ctccgggacc
    36661 ggcccttttt ttgtccttcg tcccttagac gcagcgaaat cccgcgtctc gcctaacctt
    36721 ttttgaaccc tcgctcgcaa gcgccgttct cgctccctat gtgactgcgg ggccccggga
    36781 gacgagatcc aatggcggat gaggaaaaag agccgcagcg gctgcaagtc gcgaacatgc
    36841 gccccgacga tagcggccgc ggcctcgccc ggctgccgcg cacggtgatg acctcgctcg
    36901 gcctcagcga aggggacgtg gtcgagctga ccggcaaacg agcgacgccg gcccgcgcgg
    36961 tgctctccta cccggaggat gacgggctca gcatcatccg cctcgacggc ctccagcgcg
    37021 ccaatgccga gatcggctcg ggcgatttcg tcaccattcg caagaccgaa tcgaagcccg
    37081 cgcagcgcgt cgtgttcgcg cccgcccaga agaatctgcg cctgcagggc tcggccaacg
    37141 cgctgaagcg cagcttcggc atgaagccgg tcaccgccgg ggacatcgtc gccactactg
    37201 gccagcaggc gatcaaccgc ggcgacattc cgcccgaact ccggcagatg ctgaacgcgc
    37261 ccgcctacgc gctccaggag atccggctga acgtcgtgtc gacgacgccg aagggcatcg
    37321 tccatatcga ccagaatacc gaggtcgagc tccgccccga atataccgag gccaaggacc
    37381 agcgccgcgc cgacgtcacc tatgacgatc tcggcggcat cggcgcgacc atcgaccagc
    37441 tgcgcgagat ggtcgaactg ccgctccgtt accccgagat attcgagcgg ctcggcgtcg
    37501 atccgccgcg cggcgtcctc cttcacggcc cccctggcac cggcaagacc cggcttgccc
    37561 gcgccgtcgc caacgagagc gacgccaatt tcttccacat cgccggcccc gagatcatgg
    37621 gctccgctta tggcgagagc gagaagcggc tccgcgagat tttccaggaa gccgccaagg
    37681 ccgcgccctc gatcatcttc atcgacgaga tcgattcgat cgcgcccaag cgcggccagg
    37741 tctcgggcga ggccgagaaa cggctggtcg cgcagctcct caccctgctc gacgggctcg
    37801 aaccgcgcca gaatctggtc gtcatcgcgg ccaccaaccg gcccgaggcg atcgacgagg
    37861 cgctgcggcg gcccggccgc ttcgaccgcg agatcgtggt cggggtgccc gacgagcgcg
    37921 gccggcgcga aattctcgcc atccacaccc gcggcatgcc gctcgcggac ggcatcggcc
    37981 tcgccgagct cgcgcgccgc acctacggtt tcgtcggggc ggacctggcc gcgctcaccc
    38041 gcgaggccgc gatcgaggcg gtgcggcgga tcatgcccaa gatcaacctc gaggaaggca
    38101 ccatcccggc cgaagtgctc gacaacctct cggtcacccg cgacgatttc gaggaagcgc
    38161 tgaagcgcgt ccagccctcg gccatgcgcg aagtgatggt gcagatgccc accgtccgct
    38221 gggaggatat tggcggcgtc gacgaggccc gcgagcggct gagggaaggg gtcgagctcc
    38281 cgctgaagga tcccgacgcc ttccgacggc tcggcatccg cccggccaag ggattcctgc
    38341 tctatggtcc ccccggcacc ggcaagaccc tgctcgccaa ggcctgcgcg cgcgaggccg
    38401 aggccaattt catcgccacc aaatcctcgg acctgttgtc caaatggtat ggcgaatccg
    38461 agcagcagat cgcgcgcctg ttcgcccgcg cccgccaggt cgcgccgacc gtgatcttca
    38521 tcgacgagct cgacagcctg gtccccgccc ggggcggcgg catgggcgag ccgcaggtga
    38581 ccgagcgcgt cgtcaacacc atcctcgccg aaatggacgg gctcgagacg ctgcaatcgg
    38641 tggtggtgat cggcgccacc aaccggccca acctgatcga cccggccctg ctccgccccg
    38701 gccggttcga cgagctcatc tacgtctcgg tgcccgaccg cgccggccgc cgccgcatcc
    38761 ttggcatcca caccgccaag atgccggtcg cggccgatgt cgatctcgac gatctggcgg
    38821 cgcggaccga gcgtttcacc ggcgcggacc tcgaggattt ggtgcgccgc gccggcctca
    38881 ccgcgctgcg cgaaacgctc agcgcgacca tcgtcaacgc cgaccatttc gaggccgcgc
    38941 tcaaggagac ccgcgcctcg gtgacgcccg aaatggagcg cgattatgcc gagatcaaag
    39001 ccaagctgaa gcaggaggcg atgggttcgg gcaatatcgg cttcatctcg ccgggcatgc
    39061 tcacaccgcg ggggccgaag ggcggaaacg aactttgacc cagagcgcga ggccgagcat
    39121 cagcgcgccc gtgaccagga agggcagcgg catccagatg ccgtaaagcg ccacgcccgc
    39181 ggccggggcg gcgatgaagg cgacgccgtt caccgacgtc accattcccg ccaccgcatt
    39241 ctgctcggct ggcgttacgg acagggaagc gccgctggtg aagccggggc ggaagaagcc
    39301 gaagcccaat gaggccaaag cgaagccgag cgtgatcccg taaatgccct gcgacacccc
    39361 ggtcagcaca acgcccaccg cggacagcag cgatccccac atcaccagcg cccgcggggc
    39421 gagcgcgaat tgcgggatca gccaccattg ggcgagcagg gtcgcgagcg cgccgaccat
    39481 gagtaccaag gcgatccatt gctgcgcctc gtcgagcggc agcttcagcc ggtcgatcac
    39541 caggaagccg atcacgccga gcagcgccgc atggccatgg ccgccgacga tgccgatcat
    39601 gtgccaggcc aacacgcgcg gatcgcgcca gcgcagcggc tccgccggct cggtcccctg
    39661 aaccgatccc ggcccgccgc cggcgccgcc gatcgatggg taatcggcga cgcggccgcg
    39721 cgccggtccg cttgggctgt cgtcgggcag ccgcgtcacc accgccacta gcaccaatgc
    39781 gccgatcgcg gcgaacacga tgagcggacc cgacaggccg agcggcggga agatgaacaa
    39841 aggcgccagc gccggcccga cgatggtgcc gagcccgaac gaggaggcga tcgcggccag
    39901 agcggcggtg cgctgctcgc cctcggtgcg ggcggcgaca taggcctgga cggcgggcgg
    39961 cgaagccgag ccccagccgc cgtaaatggc gcggccgagc acgaagatcc agaacacgat
    40021 ggtcggcggc agccatccct tgagccccgc cgccagcacc gaaccgcaga tcagcatcga
    40081 aatgatgaag ccgtaaagcc cgagccgcat cagcgcgcgc cggccgcgcc ggtccgacag
    40141 ccgcgcccaa tgcggcgccg tcaccaccca gatcaccgcc gacaggctga acgccaccgc
    40201 cacccacaga tcgggaatgc cgagctcccg gccgatcgaa ggcatcaggg attgcatcgc
    40261 ggtgttgccc gccgccgcca ccagcatgat cgcgaacagc aaggcgaacc gcccgcgcga
    40321 gaggctatac ccgctcaccg agcgcccacg taaaatgggt tattgatccc aatcaatgcc
    40381 atatcggccg ctatcggctt cgtccttgga aatcgcggaa aagaagcggc tcgtcccggt
    40441 ttcggcctat ttggcggggc cgagtgcctc gcccttgcca ttgtggcgtt gatttgccaa
    40501 cgcaccttcg ctgaaccact gacatggaaa cgcgcatgac cttctcggcc gaacaagcac
    40561 aaagggatcg cgccttcacg cgccgcgcgg gaggcgggcc gctttggcaa ttcctggtcg
    40621 gcttcatcaa gcatccggtg atggtggggt cggtgatccc ctcctccaag gtgctgatcg
    40681 acaagatgct ggcgccggtc gactgggaca attgcaagtt gttcgtcgaa tatggccccg
    40741 gcgtcggcac cttcacccaa cagattctcg atcggctcgc ccccgacgcg accttgctga
    40801 cgatcgacac caacccggat ttcaccgatt atctcaacgg caagttcacc gacagccgcc
    40861 tcgaagcggt taccggttcc gccgccagcg tccgcaagat catggccgac cggggcttcg
    40921 agagcgccga ttacgtcctg tccggccttc ccttctcgac ccttcctccc ggcatcggcc
    40981 ccaagatcgc gtctgagacc gcggccgcgc tccgcccggg cggagcgttc ctggtctatc
    41041 aattctcgcc caaggtgcgc gacttcatcg cgccgcattt cgagcggatc gacaagggct
    41101 tcgaatgggt caacatcccg cccgccacgc tgttctgggc ctggaaggac tgaccgactg
    41161 ccgcttcagc gcacggtgcc gccgaaattg agcctccgcg acacgttata atccatcgtc
    41221 gcgatgatga agtaggcgat gccccacttc acccggttcc accaggtccg ttgccggcgg
    41281 tgatactcgc gcgtgatctg caggctgttg gcgacctccc cgtcgatgaa gcgccgcata
    41341 gccgccgcga acgccgcatc ctcgatcctc agcatcatct ccaggttgag gtagaggctg
    41401 cgcatgtcga aattggccga gccgatatgg acgacgtcgt cgatcacgat cagcttggtg
    41461 tggagcttgg tcgcctgata ttcgtaaacc tcgacgccgc gccgcagcag ccgcgaatag
    41521 gtatggcggg cggcgccgac ggtcgcgcca ttgtccgact tggcggccgt caccacccgc
    41581 gcgcagccgc gctcgccgat cccgtagatg cgccgcagca tggtccggct cggcgcgaaa
    41641 taggcggcga tcatgtcgag ccggcacgcc cgcagcatgt cggccttgac cgattgcgcc
    41701 catgggctca gccggcgggt ggggccgccg tacagccagc gcagcggccc gacatcctcg
    41761 ctatgctgct tcagcatgtg cctgagctcg cgaatccgcc cctgcggccg cttggtccag
    41821 cgcgccagcg cgtcgaaata atgggcgagg cacacgacgc cggggccctc gacgcgaagc
    41881 ccgaggtcgc gccaggcgcc gtcggcgatg tcgccgaaat aatcgtcgga gacgttgaag
    41941 ccgccgatca gcacgcaggt gtcgtcggcc agcgccaatt tctggtggtt gcgcagcaaa
    42001 tagcgccggc cgagcttcgg cgcgaaccgg cagaaatcga ccttgccgtc ggtgagcggc
    42061 tggaagaagc tcgctttggc cgaatccgat ccgaagccgt cgaccagcaa ggtcaccttg
    42121 accccgcgct cggcggcggc gagcaaggca tcgcggaccc ggcggccgga attgtcgtcg
    42181 aggaagatgt aatagaggat gcgcaggctg cgctgcgcgc cttcgatcag cgcgatcagc
    42241 gcctccaggc gctcagggcc gtccggcagc aggctcagcc aattgtcctc gaccatcagc
    42301 gcgccgtccg gcgcctgcat cacggggtcg tgctcggcca tcgcggaccg atgtagagcc
    42361 gcccggcgcg cgcgcaaccc cgccggatca ttgacaagcc gggcccccgc ccctacctgc
    42421 cgctcttccc attcagcctt gtgacaagcg gagctgctcc atggcgcgcg ttaccgtcga
    42481 agattgcgtc gacaagattc ccaaccgttt cgacctcgtg ctgctcgccg cgcagcgcgc
    42541 ccgccagata tcgggcggcg ccgacctcac catcgatcgc gaccgcgaca agaacccggt
    42601 cgtcgcgctg cgcgagatcg ccgagcagac ggtgaagtcc aagcatctcg aggaagcgat
    42661 ggtccagaac ctccagcgcg tccagatcga cgacgacgac gctccggacg aggtcggctc
    42721 gctctccgct tcggccgagg cgctccgcct caccgccgcc gcgccgccgc gcaacaccaa
    42781 tatgggcgcc gattacgagt gatcggtgaa ggcgcgccgc ccgccggccc agccttccac
    42841 atcttcgaca cggcgatcgg ccgctgcgcc ctcgtctgga ggggcggcgc gctgatcggc
    42901 tcggcgctgc cgggcccgcg ccttgacgaa agcctcgcgc ggcgttttcc cggcgccgca
    42961 ccggctgacc cacctccgtt cgttcgatcc gccatcgacc gcatcgcggc cctgctcggc
    43021 ggcgaccgca tcgactttgc cggcatcccg ctcgatttgg gcgaggccgc gccgttcgag
    43081 cgcgccgtct acgccgccgc ctgcgcgatc ccggccggcg aggtccgcac ctatggcgag
    43141 ctcgccgcgg cgatcggcac gcccggcgcc gcccgcgccg tcggccgcgc gctcggccgc
    43201 aatccgctgc cgatcctcat cccctgccac cgcatcctgg ccgcggccgg caagagcggc
    43261 ggcttctccg ccccgggcgg cgccgccacc aaattccggc tcctcgcgct cgaacg
//
LOCUS       DASVRK010000103         4956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_204915, whole genome shotgun sequence.
ACCESSION   DASVRK010000103 DASVRK010000000
VERSION     DASVRK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204915"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VF589_03640"
     CDS             <1..995
                     /locus_tag="VF589_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8256701.1"
                     /translation="MGGVIIQREGSVEVEPGHAKTPVAWITDDDLVEGEEIVFARFRS
                     RADDEWVNTVTPRYVEPEQQWEDHAAPIRRVVADVLEDGGPREENLSLSFVTSGTQAQ
                     RCGEIKRRLGRLQRTGSVALPPPFAGLEDGDWIGWTSARRTAGLPVVFRAEAFQLGRN
                     WRNRLELREIASTVFDWTTADEIADQSVAEQQAPPVRGAPAVSDFTLSAEVLAGDGSG
                     VPALIFAGAVSARHVEAVRFEYYQGTAAPASVSDWIGTDIAGASVTRREITSVSAGTA
                     YMGALSYRTHGIFGPRRVLGPVSTDTATTGGGRWKTASGGRWKTASGGQWLLEA"
     gene            997..1587
                     /locus_tag="VF589_03645"
     CDS             997..1587
                     /locus_tag="VF589_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256702.1"
                     /translation="MADLTIAGATNAAALTGAEEMPGLQGGTDVAISVAQILAYLKGQ
                     GMPVAVARSGVAVQLTGTTTETTLATIVIPGGAIGPNGQVEVEYLVSYGAANANSKQT
                     KVKFGGTTYSGFNSTAAALSAQARIRIANRNSASSQVGQPAHQTLSGFGGSASAVATS
                     AVDTSADVTILITGTLAVNTDTMTLESYLVRVAYGA"
     gene            1602..2000
                     /locus_tag="VF589_03650"
     CDS             1602..2000
                     /locus_tag="VF589_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256703.1"
                     /translation="MPASLNIPAARNVPFANPIYLYYSGAALPLTGASIKMQIRLYPG
                     APGAALATVAAIPHTDAAAPTGADAERRLLTLFPTIAEATLAALTSGLEEPEPGDADR
                     YSYDIVVTYADAAEDTLAEGYFLLKPGVTL"
     gene            1997..4297
                     /locus_tag="VF589_03655"
     CDS             1997..4297
                     /locus_tag="VF589_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX8256704.1"
                     /translation="MSAKILEAVIPGLRGPSNYELWLADGNVGTIDDFLATLRGADGD
                     LPPASNAENIAGLATDLVTTPASDKAALDARVGPVETAIGVLDAVPFAGFVKNPIVDM
                     DFVGGRYFGERARTSLATNAPGLSFTRASTAWALGERGLYPIASGQMRLTDRGLFVGD
                     AATNIFGATNNFTSWTVASGAAVATGDDIAPTGIPYTRVTATGSSVAARYSRTFTLAT
                     SSIHTVTVPLKAGTSTKSAFFVAETGGGTNVVQLEWTWLADGGFGNMMMQTGVAEGDW
                     YYDELGNGDGLLHFRFTSHASEDGHSFRFAPDRALGTQSAQIAPFTITAGSMLAQPGR
                     NATDAEDSLTQTVDPGTHFTFGAHFFEVPLFAPAATSSATICTLDDGAGTVFNSARIQ
                     YTSDRTFRLVINSGGVKVVDIATAEPAMPNTRLRPYAVINGVEVAFYVGDNLVGTASA
                     ASMPAFTRLCVGHSGPTGFRANAFLGGAFVGKGDHSAELAEGAPELPMVVSLGQSNKA
                     GRGAYSDIDDHPTDAFLAGPQNRRGNGFIFYAPNAGTSDFTTGAAFERLWVTSNASTN
                     TMNWNPATDTFNQGQTGPEAAMLFGRGERVLVKAAIDGTQMSSDGVGSETLENSEWSS
                     SVSGELFDWAVAMAKAARTKLQAGGYKVKVEWLDIDQGEADASDITKANAHGENWWNA
                     IRKFRTEMGDPTIKAAITQLHIDNSYTYKTTIRAGERMIAGADPFAYLVDVDDIPLDP
                     LDPPHLTALGYHQRGFRIAAVMDAAR"
     gene            4335..4664
                     /locus_tag="VF589_03660"
     CDS             4335..4664
                     /locus_tag="VF589_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256705.1"
                     /translation="MLKQAALIFVLSSAAALVALVARLGLSIGNDLPPAEPAAFAAWK
                     RRRLWALVAEFSALPAFAAMWTAASYAWSLSVPVVVLGSMASGALGFGFLLQALQAIV
                     TKRLENV"
     gene            4657..4875
                     /locus_tag="VF589_03665"
     CDS             4657..4875
                     /locus_tag="VF589_03665"
                     /inference="COORDINATES: protein motif:HMM:NF037848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEX8256706.1"
                     /translation="MFDPLTLIFAAGAAGALGAGAMSTRAHAELVRMSSPVGDALRGL
                     YQGVVDEGTPAEMRELLDRLDAGEGGEA"
BASE COUNT          803 a         1671 c         1742 g          740 t
ORIGIN      
        1 cgatgggcgg cgtcatcatc cagcgcgaag gcagcgtgga agtcgagccc ggccacgcca
       61 agacgccggt ggcgtggatc accgatgacg acctggtcga gggcgaggag atcgtgttcg
      121 cgcggtttcg ctcccgcgcc gacgatgaat gggtcaacac cgtcacgccc cgctacgtcg
      181 agccggagca gcaatgggag gaccatgccg cgccgattcg gcgggtggtg gcggacgtgc
      241 tggaggacgg cgggccgcgt gaagagaatc tgtcgctgag cttcgtgacc tcgggcacgc
      301 aggcgcagcg ctgcggcgag atcaagcgcc ggctgggccg gttgcagcgg acaggcagcg
      361 tggcgctgcc gccgcccttc gcggggctgg aggatggcga ctggatcggg tggacgtcgg
      421 cgcggcgcac ggccgggctg ccggtggtgt tccgggccga ggccttccag ctcggccgca
      481 attggcgcaa ccggctggag ctgcgcgaga tcgcatcgac ggtgttcgac tggaccacgg
      541 ccgacgagat tgccgaccag tcggtggcgg agcagcaggc gccgccggtg cggggcgcgc
      601 cggcggttag cgacttcacg ctttcggccg aggtgcttgc gggggacggc tcgggcgtgc
      661 cggcgctgat cttcgccggc gcggtgagcg cgcggcacgt cgaggcggtg cgcttcgaat
      721 attatcaagg cacggccgcg ccggcgtcgg tgtccgactg gatcgggacg gacatagcgg
      781 gcgcttcggt cacccgccgg gagatcacca gcgtcagcgc cggcaccgcc tacatggggg
      841 cgctcagcta ccgcacccac gggatattcg gtccgcggcg ggtgctcggg ccggtgagca
      901 ccgataccgc cacgacgggc ggcggccggt ggaagacggc gagcggcggc cggtggaaga
      961 cggcgagcgg cggccaatgg cttttggagg catgacatgg ctgacctgac gatcgccggg
     1021 gcgacgaacg cggccgcgct caccggcgcg gaggagatgc cgggcctgca gggcggcacc
     1081 gacgttgcga tctcggttgc ccagatcctg gcctatctca agggccaggg aatgccggtc
     1141 gcggtagcca ggagcggggt ggcggtgcag ctcaccggca cgacgacgga gacgacgctt
     1201 gcgacgatcg tcattccggg cggcgcgatc ggccccaacg ggcaggtcga ggtggagtat
     1261 ctggtttcct acggggctgc gaacgccaac agcaagcaga ccaaggtcaa gttcggcggc
     1321 accacctatt cggggttcaa ctccacggcc gccgcgcttt cggcgcaggc tcggatccgt
     1381 atagccaatc gcaacagcgc cagctctcag gttgggcagc cggcgcatca gaccctttcg
     1441 gggtttggcg gcagcgcctc ggctgttgcc acctcggcgg tcgacaccag cgcggacgtc
     1501 acgatcctga tcaccggaac cctcgccgtg aacaccgaca cgatgacgct ggaaagctac
     1561 ctcgtgcgcg tcgcctacgg cgcctgacag cggagaccga gatgccggcc agcctgaaca
     1621 tcccggcggc gcgcaacgtg ccgttcgcga acccgatcta cctctattat agcggcgcgg
     1681 cgctgccgct gaccggcgcg tcgatcaaga tgcagatccg gctctatccg ggcgcgccgg
     1741 gcgcggcgct ggcgacggtg gcggcgatcc cgcacaccga cgccgcggcg ccgaccgggg
     1801 ccgacgcgga gcggcggctg ctgaccctgt tcccgacgat cgccgaggcg acgctggcgg
     1861 cgctcacgtc cgggctcgag gagcccgagc cgggcgatgc cgaccgctat tcctacgaca
     1921 tcgtcgtcac ctacgcggac gcggccgaag acacgctcgc ggagggctat ttccttctca
     1981 agccgggggt cactctatga gcgcgaaaat ccttgaagcc gtcatcccgg ggctgcgcgg
     2041 gccttccaat tacgagctgt ggctcgccga cggcaacgtc ggaacgatag acgacttcct
     2101 ggcgacgctg cgcggcgccg acggcgacct gcccccggcg agcaatgccg agaatatcgc
     2161 gggcctggcc accgacttgg tgaccacgcc cgcatccgac aaggccgcgc tcgacgcgcg
     2221 cgtcggcccg gtggagaccg ccatcggcgt gctggacgcg gtgccgttcg ccggtttcgt
     2281 caagaacccg atcgtcgaca tggacttcgt cggcggccgc tatttcggcg agcgggcgag
     2341 aacctcgctt gcgaccaacg cgcccggcct cagcttcacg cgggcatcga cggcgtgggc
     2401 gctgggcgag cggggcctct acccgatcgc gtccggacag atgcggttga ccgaccgcgg
     2461 cctgttcgtc ggcgacgccg ccaccaacat cttcggcgcg accaacaact tcaccagctg
     2521 gacggtggcc tcgggcgcgg ccgtcgcgac cggggacgac atagccccaa ccggaattcc
     2581 ctatacccgc gtcaccgcga ccggcagcag cgtggcggcc cgctactcgc ggacgttcac
     2641 cctggccacc agctccatcc ataccgtcac cgtcccgctc aaggccggca cgtcgacgaa
     2701 atcggctttc ttcgtcgccg agacgggcgg cgggaccaat gtggtgcagc tggaatggac
     2761 ctggctggcc gatgggggct tcggcaacat gatgatgcag accggcgtgg ccgagggaga
     2821 ctggtattat gacgaactgg gcaacgggga cggtctgctc catttccgct tcacctcgca
     2881 tgcaagcgag gatggccata gcttccgctt cgctccagac cgggcgctgg ggacgcaatc
     2941 ggcacagata gcgccgttca cgatcacggc cggaagcatg ctcgctcagc caggtcggaa
     3001 cgccaccgac gccgaggaca gcctgaccca gaccgtcgac ccggggacgc acttcacctt
     3061 cggcgcccat ttcttcgagg tgccgctgtt cgcgcccgcc gcaacctcct cggctactat
     3121 ctgcacgctc gacgatggcg ccggcaccgt cttcaacagc gcgcgcatcc aatatacgag
     3181 cgaccggacc ttccgcctgg tcatcaactc aggcggcgtc aaggtggtgg acattgccac
     3241 cgccgagccc gccatgccga acaccaggct gcgcccctat gcggtgatca atggcgtcga
     3301 ggtagcattc tatgtcggcg acaatctcgt cggcacggca tcggcggcct cgatgccggc
     3361 cttcacccgc ctttgcgtag gccattccgg ccccaccggc ttccgggcca atgccttcct
     3421 cggcggcgcg ttcgtcggga aaggcgacca ctcggccgaa ctggccgagg gcgcgcccga
     3481 actgccgatg gtcgtcagcc tcggccagag caacaaggcc ggcaggggcg cctattccga
     3541 tatcgacgat catccgaccg acgcattcct ggcggggccg cagaaccgcc ggggcaacgg
     3601 cttcatcttc tacgcgccca atgccggcac ctccgacttc accacgggcg cggctttcga
     3661 gcggctgtgg gtgactagca acgccagcac caacacgatg aactggaacc cggccaccga
     3721 cacgttcaac cagggccaga ccgggcccga ggccgccatg ctgttcgggc gcggcgagcg
     3781 cgtgctggtc aaggcggcga tcgacggcac ccagatgagc agcgacgggg tcggaagcga
     3841 gacgctggag aattccgagt ggagttcgag cgtttcgggc gagctattcg attgggcggt
     3901 cgcgatggcc aaggccgcgc gcacgaagct gcaggcgggc ggttacaagg tgaaggtcga
     3961 atggctcgac atagaccagg gcgaggcgga tgccagcgac atcaccaagg ccaatgccca
     4021 cggcgagaat tggtggaacg ccatccggaa gttccgcacc gagatgggcg atccgacgat
     4081 caaggcggcg atcacccagc tgcacatcga caacagctat acctataaga ccacgattcg
     4141 ggcgggtgag cgcatgatcg ccggcgcgga cccctttgcc tacctggtgg acgtcgacga
     4201 tatcccgctc gatccgctgg acccgccgca ccttaccgcg ctcggctatc accagcgcgg
     4261 cttccggatc gcggcggtca tggacgccgc ccgctgacgg cgcggccatc tcaaccccct
     4321 tccggagtaa cgccatgctg aagcaggctg cgctgatctt cgtgctgtcg tcggcggcgg
     4381 ccctggtcgc gctggtcgcg cggctcgggc tcagcatcgg caacgacctc cccccagccg
     4441 agccggccgc ttttgcggcg tggaagcggc gccggctgtg ggcgctggtc gccgagttca
     4501 gcgccctgcc cgcctttgcg gcgatgtgga ctgcagcctc ctacgcctgg agcctgtcgg
     4561 tgccggtggt ggtgctcggc tcgatggcgt cgggggcgct cggcttcgga ttcctgctcc
     4621 aggcgcttca ggcgatcgtc accaagaggc tcgaaaatgt ttgacccgct gaccctgatc
     4681 ttcgcggccg gggccgcggg cgcgctcggc gccggcgcca tgtcgacgcg agcccatgcc
     4741 gagctggtgc ggatgagctc gccggtcggc gacgcgctgc gcgggctcta ccagggcgtg
     4801 gtggacgaag ggacgccggc cgagatgcgc gagctgctcg atcggctcga cgccggcgaa
     4861 ggaggggaag catgaacgtg gagcatgtca ggatcctgca gggccggctc ggcgtcgccg
     4921 ccgacggcgg gcttggccgg gtcacttatg gcgcgc
//
LOCUS       DASVRK010000104         6939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_17857, whole genome shotgun sequence.
ACCESSION   DASVRK010000104 DASVRK010000000
VERSION     DASVRK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17857"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VF589_03670"
     CDS             <1..1170
                     /locus_tag="VF589_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8256707.1"
                     /translation="GLWQHSRMTEHLKFRHHWTFCIAALGLWVAYGWPVYKEFSAGGT
                     IRDVHVSPFWFVPFGVMGAAVLAAMVLKLRPSLHWAVLSVQLAAVVAMTVIVPWAGMS
                     SFLVIIAWQVGMSTGPVKAVSWVAFQNLVIVGTLAQALNPDLCWVLTKSGALQLLFLF
                     TAQALRREAETGRALAQTNRELRSAQAIIANTARSAERLRISRDLHDAWGHELTALGL
                     QLEIASHVTEPAKANAHVLQAKGLASALLGKVRDVVSTLRDAERCDLKESLQALAQSV
                     PTPAIHVEIAPEVRVSPDQAHALMRCAQEAVTNTVRHAEADNLWLQVTRDGEGVRLIA
                     RNDGKARPAASAPGSGLLGMRERVEALGGKLAVQAGAGLGFTVEAWLPSRAPRTT"
     gene            1181..1825
                     /locus_tag="VF589_03675"
     CDS             1181..1825
                     /locus_tag="VF589_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8256708.1"
                     /translation="MIRVVLVDDQMLVRQGVRGLLELVPDIEVVGEASDGEEALVIVP
                     ELKPDVLLLDIRMPRLSGIAVLEALRDAGTLPPTLVLTTFDDGDAAIAAIKAGAKGLM
                     LKDVSLQDLAQAIRALADNKTAFQPAMTESLMSAIRRGPSDSSDSYVAESLTVREREV
                     LHLICAGYSNKEIADLLALAEGTVKNHVSNLLLKLDARDRTRAALKALQQGHLG"
     gene            complement(1914..3254)
                     /locus_tag="VF589_03680"
     CDS             complement(1914..3254)
                     /locus_tag="VF589_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256709.1"
                     /translation="MPKPISKATVVVTGTNLHFAQPGEYSIDYLPSGNVKIQVDGGHS
                     YVMSAAEYASITSFSFADGASLGGPVAVNGVSFTEAGPGGPFEVSPNLDSLIGEMIST
                     HGIEYTAEHLLINGSAADTFKVLWDYLDDAYVAGNNYYNLPLNETFARLGLEYAEYLE
                     AGGEPLTFITAKYTSDSNSNGIPQREQSMHDNLLGNITTAAIEDRFSGALEAELLALA
                     PDEYQDRPYYDGNEPRVGGAIHDAIRAFDYDHGWDRPDYIDRSYNGLIDPLARDAAAP
                     NSMYYGNGNPVDDWNLVRHEGAQVELGLKVKHRGGDEYAEASIGPDGIAHYNVAAGPQ
                     AGSPNRAEWNFDFAATDYSPDTDFTYVVELDIDPSAGENFVTIYSSDAPLDTDLGNGS
                     TFQNSFNVAFVDTDPATPGTQHYGFGPAEFNVRISAYEGSELVLTHEVIVHVGA"
     gene            3661..6150
                     /locus_tag="VF589_03685"
     CDS             3661..6150
                     /locus_tag="VF589_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256710.1"
                     /translation="MAVIEYSFEIVDTLDLTGLSSVSGAELLMDPTTFLDAGLVVAGE
                     GATGSSSLILDMDGGFVTQAGGLTGTGPATAASLAGFVTAVDAGSDIAFEVRSNAGEL
                     IVPTTLVGEEANVSDVDVEMLNDGFALVYQMRFSDTDNDIRVSIRDADGGEVKTFSVD
                     ATGANDQAPTIAQTSGGGFVVAWHRVVGNDTQMWYALYDADGAVEKGPTLLDAAGTIN
                     RNACAISLDNGNFAIAYEDNGWAPSGNDIDITYSRFDQNGVELETADVSKNGVDDTGP
                     SLTRLRNGMIVVGSATEGAGSSDASWTLINGFDGAVLASQTLETVADESGMVVTTAGM
                     SRFAALFNDVAAGSASGRMVQPVRTTTGSDDDESLFGDWLVDVVHGGGGADYIGGGGG
                     ADRLFGQGGDDRFILSGELAAGTIVHGGAGTDTVAAGTGVGDFRRAQLVSIEKLSIEG
                     YQMVAIDTPATAIIAAAQVGDQLSDNLVVKAVSMAAETLKVVLGSASSVDLSGFTFEG
                     FDGQKDGVVIIGGNQANTILGSAIADSISGGGGKDLLRGGGGIDLVRGGAGDDIFIVD
                     AAGEAREAADQGRDTVRAAIDYTLGANLERLQLLGGGDLDGKGNALGNELYGNGGANL
                     LSGGLGRDLLNGRGGVDDLRGGAGNDVYLVNAAGEAREKAGEGVDTVRSSVSYTLGAN
                     LERLQLLGGVDTDAKGNAMANRLTGNGGDNVLNGAGGNDRMAGGGGEDGFLFNSALGA
                     GNVDMILDFDTADDSIRLDDAIFAGLTNAGRAAAFHVGKTAADASDRIIYNPETGALF
                     FDADGKGGAAQIRFATLDAGLELTAADIIIV"
     gene            6213..>6939
                     /locus_tag="VF589_03690"
     CDS             6213..>6939
                     /locus_tag="VF589_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256711.1"
                     /translation="MRGLILAALLATTAACTGEKSGEAPAAAGDSYDMAASRAKIARI
                     AMQPDTSFLNAEERQVVNLLIQAAELMNPIYQRQRFARNPEIRAEIERSGNKQLLDMY
                     DLHFGPWDTLAENHPFHGAAAMPPGAGFYPEDLTKEAFEAYLAAHPNEREALTSPYTV
                     VKRQGDRLVAVPYSQEYKQWLEPAAKLLEQAAAKTSNASLKKFLSLRAKAFRTDDYFE
                     SELAWMDLKDTPIEVAIGPYEVYT"
BASE COUNT         1186 a         2287 c         2354 g         1112 t
ORIGIN      
        1 ggtctgtggc agcattcaag aatgacagag catctcaaat tccggcatca ctggaccttc
       61 tgcatcgcgg cgctcggcct gtgggtcgcc tatggctggc ccgtctacaa ggagttctcg
      121 gccggcggca cgatccgcga cgtccatgtg tcgcctttct ggttcgtccc gttcggggtg
      181 atgggagcgg ccgtgcttgc cgccatggtc ctgaaactgc ggccgagcct gcactgggcc
      241 gtgctgtccg tccagcttgc cgcggtcgtc gcgatgacgg tcatcgtccc ctgggcgggg
      301 atgtcgtcat tcctcgtcat catcgcgtgg caggtcggca tgtcgacggg gcccgtcaaa
      361 gcggtgagct gggtcgcctt tcagaatctg gtgatcgtcg gcacgctcgc gcaggccctc
      421 aatcccgatc tgtgctgggt cctcaccaag tccggcgcgc tgcagctctt gttccttttc
      481 acggcgcagg cgctgcgacg ggaggcggaa accgggcgcg cgctcgccca gaccaaccgg
      541 gagcttcggt ccgcgcaggc catcatcgcg aacacggccc gcagcgcgga gcgcctgcgc
      601 atctcgcgcg acctgcacga tgcctggggc catgagctga ccgcgctcgg cctgcagctc
      661 gaaatcgcca gccatgtgac cgagcccgcc aaggccaacg cgcatgtgct gcaggcgaag
      721 ggcctcgcca gcgccctgct cggcaaggtc cgcgacgtgg tctcgacgct gcgcgatgcc
      781 gaacgctgcg atctgaagga atcgttgcag gcgctggcgc aaagcgttcc cacgccggcg
      841 atccatgtcg agatcgcgcc cgaagtgcgg gtcagccctg accaggcgca cgcgctgatg
      901 cggtgcgcgc aggaagccgt gaccaacacc gtccggcacg ccgaagcgga caatttgtgg
      961 cttcaggtga cccgggacgg cgaaggcgtt cgccttatcg cccgcaacga cggcaaggcc
     1021 cggcccgccg cgtccgctcc cggctccggc cttctcggga tgcgtgagcg ggtggaggct
     1081 ctcggcggca agctggcggt gcaggccggc gccgggctcg gcttcacggt cgaggcctgg
     1141 ctgccttcgc gggcgccgcg gaccacatga tccggcccgc atgatcaggg tcgttctcgt
     1201 cgacgaccag atgttggtcc gccagggcgt gcgcggcctg ctcgagctgg tgcccgacat
     1261 cgaagtggtg ggcgaggcga gcgacggcga ggaggcgctg gtcatagtgc cggagctgaa
     1321 gcccgatgtg ctgctcctcg acatccgcat gccccggctc agcggcatcg ccgtgctcga
     1381 ggcgctgcgc gacgccggca cgctgccgcc gaccctcgtc ctcaccacct tcgacgacgg
     1441 cgacgccgcg atcgcggcga tcaaggccgg cgccaagggc ctgatgctga aggacgtctc
     1501 gctccaggac ctcgcccagg cgatccgcgc gctggccgac aacaagacgg cgttccaacc
     1561 ggccatgacc gagagcctga tgtcggcgat ccgccgcggc ccgtcggatt cgtccgacag
     1621 ctacgtcgcc gagtcgctga ccgtccgcga gcgcgaggtg ctgcatctga tctgcgccgg
     1681 ctacagcaac aaggaaatcg cggacctgct cgcgctggcc gagggcacgg tgaagaacca
     1741 cgtctccaac ctgctcctga agctcgacgc ccgcgaccgg acccgggcgg cgctcaaggc
     1801 gctgcagcag ggccatctcg gctaagcgcc ggagcgcctt cgccggggca cgctcacgct
     1861 gaaaaaaagg ggctcccgaa ggagccccct gaagttcccg agatcgcggc cggtcaggcg
     1921 ccgacgtgga cgatcacttc gtgcgtcagc accagctccg aaccttcata ggcgctgatc
     1981 cggacgttga actcggccgg gccgaagccg taatgctggg tcccgggcgt ggccgggtcg
     2041 gtgtccacga aggcgacgtt gaacgaattc tggaaggtcg atccgttgcc gagatcggtg
     2101 tcgagcggcg cgtcggacga atagatggtg acgaaattct cgcccgcgct cgggtcgatg
     2161 tcgagctcga cgacataggt gaagtccgtg tccgggctgt aatcggtggc ggcgaagtcg
     2221 aaattccact cggcccggtt tgggctgccg gcctgcggcc cggccgcgac attgtaatgg
     2281 gcaatgccgt cggggccgat cgaggcttcg gcatattcgt cgccgccgcg atgcttgacc
     2341 ttgagcccga gctcgacctg cgcgccttcg tggcggacga ggttccagtc gtcgaccgga
     2401 ttgccgttgc cgtaatacat gctgttcggc gccgccgcat cgcgggcgag cgggtcgatc
     2461 agcccattat agctgcggtc gatataatcg ggccggtccc agccgtgatc gtaatcgaag
     2521 gcgcggatcg cgtcgtggat cgcgccgccg acgcgcggct cgttgccgtc ataataaggc
     2581 cggtcctgat actcgtccgg ggcgagcgcg agcagctcgg cctcgagcgc gccgctgaag
     2641 cggtcctcga tcgccgccgt ggtgatattg ccgagcaggt tgtcgtgcat cgactgctcg
     2701 cgctgcggga tgccgttgga attgctgtcg gacgtatatt tggcggtgat gaaggtgagc
     2761 ggctcgccgc cggcttcgag atattcggca tattccagcc caagccgcgc gaacgtctcg
     2821 ttgagcggca aattgtagta attgttgccc gcgacatagg cgtcatcgag ataatcccac
     2881 agcaccttga aggtgtcggc ggcgctgccg ttgatgagca gatgctcggc cgtatattcg
     2941 atgccgtggg tcgagatcat ctcgccgatc agcgaatcga gattggggct gacctcgaac
     3001 gggccgcccg ggccggcttc ggtgaagctc accccgttga ctgctaccgg gccgccgagc
     3061 gaggcgccgt cggcgaagct gaacgaggtg atcgaggcat attcggccgc gctcatcaca
     3121 taggaatggc cgccgtcgac ctggatcttg acgttgccgg acgggagata gtcgatcgaa
     3181 tactcgccgg gctgcgcgaa atgcaggttg gtgccggtga cgacgacggt cgctttggaa
     3241 atcggcttgg gcatgattgg tcttccccca cttggagctg ctggaatgta agcggacagg
     3301 cgacgcggct ccccccgggg ccgactcgtc gcgaaaggtg gatcgagcgc gcatattgac
     3361 gccgcaaggc cgggccgagt caagcggccg tcccggatcg ggagctggcg gcggctaaaa
     3421 atcctcgtgc cgtcccggca agacaaaccg ccccccgcaa cttgccgccc tgagcccgaa
     3481 accttgagct atgttaggcg cgagcgttgg tcgcgggaat tgcggccttg gtcagcttga
     3541 tcgccggggc cggaactttg gtcgatcaaa ggcatagccc agtcccaatc ctgttaggcc
     3601 ggcgcgcaga acaaggccgg ccgctcggac cggtgagccg aaacagggga agatcgcgcc
     3661 atggccgtca tcgaatatag cttcgaaatc gtcgacacgc tggacctcac cggcctttcc
     3721 tcggtcagcg gggccgagct gctcatggat cccaccacct tcctggacgc cggcctggtg
     3781 gtcgccggcg aaggcgcgac cggaagctcg tcgctgatcc tcgacatgga cggcggcttt
     3841 gtaactcagg cgggcggatt gaccgggacc ggcccggcga cggccgcatc cctggccggt
     3901 ttcgtcacgg cggtggatgc cggaagcgac atcgccttcg aagtgcgtag caatgccggc
     3961 gagctcatcg tccccaccac cttggtgggg gaagaggcga acgtcagcga cgtcgatgtc
     4021 gaaatgctca acgacggctt tgcgctcgtc taccagatgc gcttttcgga caccgacaac
     4081 gacatccggg tcagcatccg cgatgccgac ggcggcgaag tgaagacgtt cagcgtcgac
     4141 gccaccggcg ccaacgacca ggccccgacc atcgcccaga cctccggcgg cggcttcgtg
     4201 gtggcgtggc accgggtcgt cggcaacgac acccagatgt ggtacgcgct ctacgacgcc
     4261 gacggcgcgg tggagaaggg gccgacgctg ctcgatgcgg ccggcacgat caaccgcaac
     4321 gcctgcgcca tcagcctcga caacggcaat ttcgcgatcg cctatgaaga caatggctgg
     4381 gcgcccagcg gcaacgatat cgacatcacc tattcgaggt tcgaccaaaa cggcgtcgag
     4441 ctcgaaaccg cggacgtttc caagaatggc gtcgacgata ccgggccgag cctcacccgc
     4501 ttgcgcaacg ggatgatcgt ggtcggctcc gccaccgaag gggcgggcag tagcgacgcc
     4561 agctggacgc tgatcaatgg gttcgacggg gcggtgctcg ccagccagac attggaaacc
     4621 gtggccgatg aaagcgggat ggtggtgacg acggccggca tgagtcgctt cgccgctctg
     4681 ttcaacgacg tcgccgcggg ttcggcatct gggcgaatgg tccagccggt gcgcaccacg
     4741 accgggagcg acgacgacga atccctgttc ggcgattggc tggtggacgt ggtgcatggc
     4801 ggcggcggag cggactatat tggcggcggc ggcggtgcgg accggctgtt cggccagggc
     4861 ggcgacgacc gcttcattct ctcgggcgag ctcgccgccg gcacaatcgt ccacggcgga
     4921 gcgggcaccg acaccgtcgc ggcgggaacc ggggtcggcg acttccggcg cgcgcaattg
     4981 gtttcgatcg agaaactcag catcgaaggt taccagatgg tggcgatcga caccccggcc
     5041 accgcgatca tcgcggccgc ccaggtcggc gaccaattgt cggacaatct agtcgtcaag
     5101 gccgtgtcga tggccgccga aaccctgaaa gtagtcttgg gatcggccag ttcggtcgat
     5161 ctctccggct ttacgttcga gggtttcgac gggcagaagg acggcgtcgt catcatcggc
     5221 ggcaaccagg cgaacacgat cctcgggtcg gccatcgccg acagcatcag cggcggcggc
     5281 ggcaaggacc tgctgcgcgg cggcggcggc atcgatctcg ttcggggcgg cgccggcgac
     5341 gacatcttca tcgtcgatgc cgccggcgag gcccgcgagg ccgccgacca gggccgcgac
     5401 acggtgcgcg ccgcgatcga ttacacgctc ggcgccaatc tcgagcggct gcagctgctc
     5461 ggcggcggcg atctcgacgg caagggcaat gcgctgggca acgagcttta cggcaatggc
     5521 ggcgccaatc tgctgtccgg cggcctcggc cgcgacctgc tcaacggccg cggcggcgtc
     5581 gacgaccttc gcggcggcgc cggcaacgac gtctatctgg tcaacgccgc cggcgaggca
     5641 cgcgagaaag cgggcgaggg cgtcgatacg gtgcggtcct cggtgagcta cacgctcggc
     5701 gccaatctcg agcgcctgca attgctcggc ggcgtcgaca ccgacgccaa gggcaatgcc
     5761 atggccaacc gcctcaccgg caatggcggc gacaatgtgc tgaacggcgc cggcggcaac
     5821 gaccgcatgg ccggcggcgg cggcgaggac ggcttcctgt tcaacagcgc gctcggcgcc
     5881 ggcaatgtcg acatgatcct cgatttcgac accgccgacg acagcatccg gctcgacgac
     5941 gcgatcttcg ccggcctcac caatgccggc cgcgccgccg ccttccacgt tggcaagacc
     6001 gcggccgacg cgtccgaccg gatcatctac aatcccgaga ccggcgcatt gttcttcgac
     6061 gccgacggca agggcggcgc cgcccagatc cgcttcgcca ccctcgacgc cgggttggag
     6121 ctcaccgccg ccgacatcat catcgtgtga cgccgctctt cacgcccgcg cgccggcccg
     6181 atagagctgg cgggggacgt gaggagagcg tgatgcgtgg attgatattg gccgccctgc
     6241 tggcgacgac cgccgcctgc accggagaga aaagcggcga ggccccggcg gcggccggcg
     6301 acagctacga catggccgcc tcgcgcgcca agatcgcgcg cattgccatg cagccggaca
     6361 ccagcttcct caacgccgag gagcggcagg tcgtcaacct gctgatccag gcggccgagc
     6421 tgatgaatcc catctaccag cgccagcgct tcgcccgcaa tccggagatc cgggccgaga
     6481 tcgagcgctc cggcaacaag cagctgctcg acatgtacga cctccatttc ggcccgtggg
     6541 acacgctcgc cgagaaccac ccgttccacg gcgccgcggc gatgccgccg ggcgccggct
     6601 tctatccgga ggatctcacc aaggaggcgt tcgaagccta tctcgccgcc cacccgaacg
     6661 agcgcgaggc gctgaccagc ccctatacgg tggtcaaacg gcagggcgac cggctggtcg
     6721 cggtgcctta cagccaggaa tataagcagt ggctggagcc ggccgccaag ctgctcgaac
     6781 aggccgcggc gaaaaccagc aatgccagcc tgaagaaatt cctcagcttg cgggcaaagg
     6841 ccttccgcac cgacgattat ttcgaatccg agctggcctg gatggacttg aaggacacgc
     6901 cgatcgaggt cgcgatcggg ccttacgagg tctacaccg
//
LOCUS       DASVRK010000105         5514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_578571, whole genome shotgun sequence.
ACCESSION   DASVRK010000105 DASVRK010000000
VERSION     DASVRK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578571"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..973)
                     /locus_tag="VF589_03695"
     CDS             complement(122..973)
                     /locus_tag="VF589_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX8256712.1"
                     /translation="MSKHFVQNAALRALLLGGYRAYARAQLWRSGPRIFVNSIPKAGT
                     HLLAAELDNFRSLQNSRLHLMNQKLRLPGPRTPEAHGRFDIAKIAREIGTVRGGQYFT
                     AHLFWSEELERLLAEQGVRTIFMARDPRAILVSRLHYTLGLKRHWLNAHLAENFATDE
                     ARYHFLLDGHAGDPFVLPVEHLLAGHIPWTRSPTTLTVKFEELVGERGGGTATAKLEA
                     LRRIADHCGLPTEELDRFAASAAKATPTLRRGKADAWRSELPRSIAERVNREWGPILA
                     AFGYPAD"
     gene            complement(973..2262)
                     /locus_tag="VF589_03700"
     CDS             complement(973..2262)
                     /locus_tag="VF589_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX8256713.1"
                     /translation="MNAAAAIIGHVRGRFRSQAGRRLVWSMAAAIAARGSLLVLAVVL
                     ARQLGPSGYGTFTFATGMALLAAQIAVLGWPQLMNRMIPPLLRDRDWPRLKGLRDMGD
                     AVVFTASLGAALILAALSLLFPKLSEGFLLGALLTVPFALAILRRQQLAAVRLPGMGL
                     LFDQGFGAILTVAVLLALGGGQVLGEAVLFFGAMIVVGVVVTTIVFRRRLPPELAGAE
                     RRFEFRAWMAIALPILVGLSSRIFMNKTDVLMLAPLSNMHEVGLYGAAFRITYVLTFP
                     QVVMMSVVTPLFSEAFAHERHRQVRRLLGGALLFAAVTALPFSLALVLFPELVMTSLF
                     GEAFAPAAPTLALLAVGQLATSLAIPFGGLLTMGGRERVFGGINLAGLALHVALNFLL
                     VPTMGATGAAIATLCVAVSLLVTQVIANRAILAGRQR"
     gene            complement(2259..2960)
                     /locus_tag="VF589_03705"
     CDS             complement(2259..2960)
                     /locus_tag="VF589_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256714.1"
                     /translation="MRNSPWVLGTAWKHRRWVKPMQRHLGDRKVAEPGDDVVIEGYVR
                     SANTFATDAFAASQDRPVSIATHFHSPAQFGLARKYKIPAMLLLRDPVAAALSYVVFN
                     EQAYNAERALRFYIHFHRPLLTISDSFVVAPFEEVTSDFGKSMKRLNDRFGTDYKLFT
                     HNEESQQRLFDAMAERLRQRELARGTSLKLRMNFPHAEKEKKRREIAQVFEHPSLDRL
                     KAKARRQYDRLMALV"
     gene            complement(3051..4025)
                     /locus_tag="VF589_03710"
     CDS             complement(3051..4025)
                     /locus_tag="VF589_03710"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8256715.1"
                     /translation="MGCWFASAAPPPDCRRDANVAARSVVGYSRAAGKGRHRADAEGG
                     ELSRPGFLIVGAMKGGTTTLYHDLSKHPDLCLPEPKEPEILVRHEDPEAARAAYAAHF
                     RAATQGQICGEASTAYTKRPLHEGVAAKAKRVCGADLKIIYMRRDPVSRALSQYKHEK
                     QHGLVAEPFDEAVRRHGRFIDFGRYDWQIAPWAEAFGRERLLQLDLESYSADRVATLA
                     RVLEFIGVDPARIRDIDPQSVSNSADEQKHIANPVLRSLVYSDLYQHVIKPRLPRSFR
                     ERARRSILPPPEKAKIEVQPATARFIMDQLDRGRPEAVSAGAGMPAEL"
     gene            complement(3988..4344)
                     /locus_tag="VF589_03715"
     CDS             complement(3988..4344)
                     /locus_tag="VF589_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8256716.1"
                     /translation="MALDRKAVAILPCNDLAVSQAFYERLGFALAADHGDYRILDDGR
                     GWHLHLRAAEPGSVIPERNAGGLYLYAEDVDRVADRVRDLIIEPDTPAVKPWGMYEFA
                     VSDPDGVLVRIGRPAS"
     gene            4486..>5514
                     /locus_tag="VF589_03720"
     CDS             4486..>5514
                     /locus_tag="VF589_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8256717.1"
                     /translation="MFGFEAYHILLAALGISIVLAFWLPRFVSGREPATSALLIGAGL
                     VSFAFVPGMPAALDPVVSPRIWEIASELCVIIGLFGTGLRIDRFVERGKWMPTARLLV
                     VAMPLTIILLALFGWAVAGMTLAGSLLLGAILAPTDPVLAGDVQVGPPHEGGEHPVRY
                     ALTTEAGLNDGLAFPFVHLGVLVATAGGLSFALAGEWLLRDLIYRVAAGAAGGAATGW
                     ILGKVLFDWPRENALSKTGSGVVALAGVLLAYGLTEVAEGYGFIAAFTAGYTLRRSET
                     HHRFHRRLHDFSESIEHALTAMILVGLGAAMPMLWPHLDWSHVSIALMLVFIIRPLAA
                     WGSLRGTPL"
BASE COUNT          867 a         1875 c         1877 g          895 t
ORIGIN      
        1 ggcgatcgtc ggcatcgcgg tgcacaagct tatcgagaag ccggccatgg agctggtcaa
       61 ccgcgcgatg cggcggcgcc ggccgaccct cgccgcgact tgagtcccgc ccaggcgggg
      121 ctcagtcggc aggatagccg aaggccgcca ggatcgggcc ccactcgcga ttgacccgct
      181 ccgcgatcga acgcggaagt tccgatcgcc acgcgtcggc tttgccgcgc cgcaaggtcg
      241 gcgtcgcctt ggcggcgctg gcggcgaagc ggtcgagctc ctccgtcggg agcccgcaat
      301 gatccgcaat ccgccgcaac gcctccagct tcgcggtcgc ggttccgccg ccgcgctcgc
      361 cgaccagctc ctcgaacttc acggtcagcg tggtggggct cctggtccaa ggtatatgcc
      421 cggccagcaa atgctcgacc gggagcacga acggatcgcc cgcatggccg tcgagcagga
      481 agtggtagcg cgcctcgtcg gtggcgaagt tttcggcgag gtgggcattg agccaatggc
      541 gcttcagccc gagcgtataa tggagcctcg agaccaggat ggcgcggggg tcgcgcgcca
      601 tgaagatggt ccgcacgccc tgctcggcga gcagccgctc cagctcctcc gaccagaata
      661 aatgggcggt gaaatattgc ccgccgcgga cggtgccgat ctcgcgggcg atcttggcga
      721 tgtcgaaccg gccatgcgct tccggggtgc gcggacccgg cagcctcaat ttctggttca
      781 tgaggtgcag gcgggaattc tggagcgacc ggaaattgtc gagctcggca gccagcaaat
      841 gggttcccgc cttggggatg ctgttcacga agatccgcgg ccccgatcgc cacagctgcg
      901 cgcgggcata agcgcgatag ccgcccagca gcaaagccct gagcgccgca ttctgcacga
      961 aatgcttgga catcagcgct gccgccccgc caggatcgcg cggttcgcga tcacttgcgt
     1021 caccagcagc gagacggcca cgcacaatgt cgcgatggcc gcgccggtgg cgcccatggt
     1081 cggcaccagc aggaagttga gcgcgacgtg gagcgcgaga ccggcaaggt tgataccgcc
     1141 gaacacacgc tcgcgcccgc ccatggtgag caggccgccg aacggaatgg cgaggctggt
     1201 ggcgagctgc cccaccgcca gcagcgccag cgtcggcgcc gccggcgcga atgcctcccc
     1261 gaacaggctg gtcatcacca gctccgggaa cagcaccagc gctagcgaaa atggcaaagc
     1321 ggtgacggcg gcgaacagca aagcgccgcc gagcagccgc cgcacctgcc ggtgccgctc
     1381 atgggcgaag gcctcgctga acaaaggcgt gacgacgctc atcatcacca cctgcgggaa
     1441 ggtcagcacg taggtgatcc ggaacgcggc gccgtaaagg ccgacctcgt gcatgttcga
     1501 caacggcgcc agcatcagca cgtcggtctt gttcatgaag atgcgcgacg acaggccgac
     1561 caggatcggc agcgcgatcg ccatccaggc gcggaactcg aaccgccgct cggcccctgc
     1621 cagctcgggt ggcagccgcc gccggaacac gatggtggtg acgacgacgc cgaccacgat
     1681 catcgcgccg aagaagagca cggcctcgcc gagcacctgg ccgccgccca gggcgagcag
     1741 cacagcgacg gtgaggatcg cgccgaaccc ctgatcgaac agcagcccca tgcccggcag
     1801 ccgcaccgcg gccagctgct ggcgcctgag gatcgccagc gcgaacggca ccgtgaggag
     1861 ggcgccgagc aggaagcctt cgctcagctt cgggaatagc aagcttagcg ccgcgaggat
     1921 cagggccgcg ccgaggctgg cggtgaacac caccgcatcg cccatgtcgc gcagcccctt
     1981 gagccgcggc cagtcgcggt cgcgcagcag cggcgggatc atccggttca tgagttgcgg
     2041 ccagccgagc accgcgatct gcgccgccag cagcgccatg ccggtcgcga aggtgaaggt
     2101 gccgtagccg gacgggccga gctggcgcgc gagcaccacc gccagcacca gcaggctgcc
     2161 gcgcgccgcg atcgccgccg ccatcgacca gaccaggcgc cgccccgcct gcgatcggaa
     2221 gcggccgcgg acgtggccga ttatcgccgc cgcggcgttc ataccaaggc catcagcctg
     2281 tcatattggc ggcgcgcctt ggccttcagc cggtcgaggg aagggtgctc gaacacctgg
     2341 gcgatctcgc gccgcttttt ctccttctcc gcatggggga agttcatccg aagcttgagg
     2401 ctggtgccgc gcgccagctc gcgctgccgc aaccgctcgg ccatcgcgtc gaacagccgt
     2461 tgctgcgatt cctcattatg ggtgaagagc ttgtagtcgg tgccgaagcg gtcgttcagc
     2521 cgcttcatcg acttgccgaa atcgctcgtc acttcctcga acggcgccac cacgaagctg
     2581 tcgctgatcg tgagcaaggg ccggtggaag tggatgtaga agcgcagcgc ccgttcggca
     2641 ttataggcct gctcgttgaa caccacatag gacagcgccg ccgcgaccgg gtcgcggagc
     2701 agcagcatgg ccgggatctt gtatttgcgc gcgaggccga actgggccgg cgaatggaaa
     2761 tgggtggcga tcgagaccgg ccggtcctgg ctcgccgcga aggcgtcggt ggcgaaggtg
     2821 ttggccgacc gcacatagcc ttcgatgacg acgtcgtcgc ccggctcggc gaccttgcgg
     2881 tcgcccagat gccgttgcat cggcttcacc cagcgccggt gcttccaggc cgtgcccagc
     2941 acccacgggc tgttccgcat gagcattctc atgtcgtaca atggcatcat gggcaaagga
     3001 ctccggatat tggtggcgtg ttgacgtggg cgcgggcgag gggaggcggg ctaaagctcc
     3061 gccggcatcc cggcgccggc ggataccgct tcggggcgcc cccggtccag ctggtccatg
     3121 atgaacctgg cggtcgccgg ctggacctcg attttcgcct tttcgggcgg cggcaggatc
     3181 gatcgccgcg cccgctcgcg aaacgagcgc ggcagcctcg gcttgatgac atgctggtag
     3241 aggtctgaat agaccaggga ccgcagcacc gggttggcga tgtgcttctg ctcgtcggcg
     3301 gaattggaga cggattgggg gtcgatgtcc ctgatccggg ccgggtccac cccgatgaat
     3361 tcgagcaccc gcgccagcgt ggccacccgg tcggccgaat agctttcgag gtcgagctgg
     3421 agcagccgtt cgcggccgaa cgcctcggcc cacggcgcga tctgccagtc gtagcggccg
     3481 aagtcgatga accgcccgtg gcggcgcacg gcttcgtcga acggctccgc gaccagcccg
     3541 tgctgctttt catgcttata ctggctgagc gcgcgcgaga ccgggtcgcg ccgcatgtag
     3601 atgatcttga ggtccgcgcc gcacacccgc ttcgccttgg cggcgacgcc ctcgtgcagc
     3661 ggccgcttgg tgtaggcggt cgacgcctcg ccgcaaatct ggccttgggt cgcggcccgg
     3721 aaatgcgccg cataggcggc ccgcgccgcc tccggatcct cgtgccggac cagtatttcc
     3781 ggctccttcg gctcagggag gcaaaggtcc ggatgtttgg acagatcatg gtataaggtg
     3841 gtggtgccgc ctttcatggc gcccacgatc aagaaacccg gcctgctcaa ttccccacct
     3901 tccgcgtccg cgcgatgccg gcctttcccg gcggcgcggc tgtagccgac aacgcttctt
     3961 gcggcaacgt tcgcgtcccg gcgacagtca ggaggcgggg cggccgatgc gaaccagcac
     4021 cccatccggg tcgctgaccg cgaattcgta catgccccag ggcttcaccg ccggcgtgtc
     4081 aggctcgatg atgaggtcgc gaacccggtc cgccacgcga tcgacgtcct cggcatagag
     4141 atagaggccg ccggcgttgc gctcggggat caccgagccc ggctcggccg cgcgcaaatg
     4201 caaatgccag ccgcgcccgt cgtcgaggat gcggtaatcg ccatgatcgg cggccagggc
     4261 aaaccccagc cgctcgtaaa atgcctggct taccgcgaga tcattgcagg gaaggatcgc
     4321 caccgccttg cggtcgagcg ccatgcgcac ctccgttcgt tgcgccgtgg gtcgcacgcg
     4381 gtgccggtgc ccgcaaggcg cttgtcggaa cgtgtctttc actgccgggt tgggccggaa
     4441 gcgaagccgt agcttgcagc acggccgggg tgggagtttc gccaattgtt cggcttcgag
     4501 gcctatcata ttctgctcgc ggcgctgggc atcagcatcg tgctggcctt ctggcttccc
     4561 cgcttcgttt cggggcgcga gccggcgacg tcggcgctcc tcatcggcgc cggcctggtg
     4621 tcgttcgcct tcgtaccggg aatgccggcc gcgctcgatc cggtggtctc gccgcgtatc
     4681 tgggagattg cgagcgagct ctgcgtgatc atcggcctgt tcggcaccgg cctcaggatc
     4741 gaccgattcg tcgagcgcgg caagtggatg ccgaccgcgc gcctgctggt cgtggccatg
     4801 ccgctgacca tcatcctgct ggctctgttc ggctgggcgg tcgccggcat gacgctggcc
     4861 ggcagtctgc tgctcggcgc gatcctcgcc ccgaccgacc ccgttctggc cggcgacgtc
     4921 caggtcggac ccccgcatga aggcggcgag catccggttc gctacgccct caccaccgag
     4981 gcggggctga acgacggcct cgccttcccg ttcgtccatc tcggcgtgct ggtcgcgacg
     5041 gccgggggct tgagcttcgc ccttgccggc gagtggctgc tgcgcgacct catctaccgc
     5101 gtcgcggccg gggccgccgg cggagcggcg accggctgga tcctcggcaa ggtcctgttc
     5161 gattggccgc gcgagaatgc gctttcgaag accggctcgg gcgtggtcgc gctcgccggc
     5221 gtgctgctcg cttatgggct taccgaggtg gccgaaggct acggcttcat cgccgccttc
     5281 accgccggct acacgcttcg ccgttcggag acgcaccacc gcttccaccg ccggctccac
     5341 gatttttccg aatcgatcga gcatgcgctg accgcgatga tcctggtggg gctgggcgcg
     5401 gcgatgccga tgctgtggcc gcatctcgat tggagccacg tctcgatcgc tttgatgctt
     5461 gtattcatta tccgcccgct cgccgcctgg ggctcgctgc gcggtacgcc gctg
//
LOCUS       DASVRK010000106        16950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_18096, whole genome shotgun sequence.
ACCESSION   DASVRK010000106 DASVRK010000000
VERSION     DASVRK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16950
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18096"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VF589_03725"
     CDS             <1..1246
                     /locus_tag="VF589_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8256718.1"
                     /translation="RQPFSNRYGLAYATLRGEWRGYRLTSAFSLVGQDLDESFDATLP
                     EGPTELFRQRNRLEMVSLETRIAREAQGGGWLAGLSLLANRTELDRAVLPPGRLLALP
                     GVANRLAEQTLFAEATRPLWSGLSLTLGARLSHVSFTGSATEPQGPAPDGIGRIEGDE
                     WRLLPAAALGASLGSDLRLYLRYQESFRPGGAIVRTNAAQRLESDRIQAWEAGLRFGH
                     GRSARLSGAVSASYSRWNNVQADLIDTTGLPTTANLGDGEIWTLDGRLEWRATPALRL
                     GVAAVVSRSRVSNPDPGIIFIEDEPLPNVPDLTATASVDYRRPLGASRQLELSARARY
                     VGKSTLGIGPILERAQGDYVDTSLEARITGGRWIWFVAGTNLLNTEGNRFALGSVLTL
                     VSEDQVTPLRPLTVRVGVETRF"
     gene            complement(1448..2788)
                     /locus_tag="VF589_03730"
     CDS             complement(1448..2788)
                     /locus_tag="VF589_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8256719.1"
                     /translation="MRFPIGAALLSLASAALAAAQPGTAPPSRPSAAPARIPAPAVDH
                     HLHLLTPEAAAILVPPLLPPVALPQELASLLRARTEQPSRDLYTRDAIILDIRGPTWV
                     RGRAAIQAFLGGAQRGSRLVPVSYSLVGQGAHVAGLIVRGEGEAARPVRTFHLSLTKE
                     GAGWLIAAESWSERAPPTPRATFAQQLVAEMDAAGVRRGVVHSPGYFFASPLLPKPYP
                     REAALVSAQNDWIVRQVAQYPGRLVAFCGVNPLKDHALAEISRCGRIEQVRGIKLHLG
                     NSGVDLRNRDHVERLAGVFRAADAAKLAIAIHLWNGPRYGSADAAIFLADILPMAPDV
                     AVQVMHLAGGGGAYGPDEALTPFAAAIAAGSPQARNLYFDVAAIVTGRETPEQLALIA
                     RRIRELGVRRILFGTDRDGLNAMPPAEAWAAFSRLPLDEAEFRTIAANAAPYLD"
     gene            2865..3470
                     /locus_tag="VF589_03735"
     CDS             2865..3470
                     /locus_tag="VF589_03735"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8256720.1"
                     /translation="MIETVSLDRQRQQDLPLRARKKLDSMRRAQAAAIRLFARRGFGG
                     VSMEEVAAASDVSPASLYRYFGTKENLIIWDEDDDPLFDRVARHLRTMPPLQAFAAAI
                     VEALGPSFETGREARSGRLRLIFREPALRSAFRENGRLLAQGLARVFASGRAAAAPSL
                     GDLALAAAAAAILEVAVERWATGGAETRLAQIISEAFECLA"
     gene            3458..4543
                     /locus_tag="VF589_03740"
     CDS             3458..4543
                     /locus_tag="VF589_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256721.1"
                     /translation="MPRLAPRSGAMRLLRRAAAVLLLLLLLAALLLAVEFGLRRPYTP
                     ALPDEPPANAIASLEQLPIRGVPQSVLIRGRDRTKPILLFLHGGPGMPVMFLGHAFQR
                     GLERDFVVVHWDRRGAGRSYDSWTPEASSVRATLDDLHALTAQLKRRFRQDRVYLVGH
                     SWGTYLGLLAVHERPDLFSAYIGTGQMAADPERVRAARQAFFEREARARGDEEMLGRL
                     AAGGEITEDDLFRYGAELRGAESFWPILMLGLRAPEYRLRDVLNVPKGSERLLRTMRY
                     DVIQGPLDRNLVEFRVPIFFFLGRHDWNEPSTLAADYLRRIRAPDKGLVWFEHAAHFP
                     FLEQPERFHGAMSALHRRLTDRRPPTP"
     gene            4691..5161
                     /locus_tag="VF589_03745"
     CDS             4691..5161
                     /locus_tag="VF589_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256722.1"
                     /translation="MTFTLQGDTLVNGTSAGDQYNSSLARLGDGSYVVVWQTGDAAPN
                     ALFGQLHASDGTALGGEFQINAGTGGTYVEPRVAALSGGGFAVVWSNSSDLSYRLFEA
                     DGTPLGGDLILTTADEIGGYDWQIDVAPTDDGLVVTFLRPTYGDIFSVGRAYRQ"
     gene            5121..5798
                     /locus_tag="VF589_03750"
     CDS             5121..5798
                     /locus_tag="VF589_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256723.1"
                     /translation="MATYSRSDALTGNDGSNGLDGRGGADRLEGAGGNDRYFVDHAGD
                     EIVEASEGGSDAVFSSVSHQLADEVEALRLTGASAINGSGNGSGNRLFGNAAANRLDG
                     GDKSDGLFGGAGNDLLTGGAGADRFYFDTALDAAANVDAISDFADGDRIFLARSIFTE
                     IAAGTLDPGAFCAGAAAADDSDRIVYDVSTGNIFYDADGSGSGAAMLFATVAVGTELE
                     AADFIGY"
     gene            complement(5792..6673)
                     /locus_tag="VF589_03755"
     CDS             complement(5792..6673)
                     /locus_tag="VF589_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8256724.1"
                     /translation="MNGVRLWYRVAGRSGGIPLVFLHGGPGQGSQTFQAVGGPELEKT
                     QRLVYLDQRGSGRSDRPQDPSYYSIDIMVEDVERLRRHLGVPKIALLGHSFGTQLALE
                     YAARYPRQTHAVVLAAAAAHLTRSIDLQCERLAVEDPEAYARAAAGIRAGVVPRCNTM
                     SAYTGDAATAFAHRNLFPDLATARRVTELDSAGGLGATGEAASLLFRKGYLQYRFSRA
                     ANVVAPVLIIAGGRDYQTAIEPQRDLVKELPRGRLLEYPEAGHFMFVETPERFARDVA
                     AFLRRPPARARSAAPAQ"
     gene            complement(6894..7706)
                     /locus_tag="VF589_03760"
     CDS             complement(6894..7706)
                     /locus_tag="VF589_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8256725.1"
                     /translation="MLLPAFLRRDFRPGRRVFYGAALAILFVAYSLVLALTGNWPLSR
                     VIANALANVVPIALLGLASRQVIRSMADKPLVTKMVGHLALGLVFTIAWFWLLMVLLG
                     AVAGRNAIEFSVRPFLGPAATWQLFQGLTVYAAITVAVHAEMLAERSPIPAPEREGGS
                     ASSQPPRLFVKHEEELRPLDTDRIILIRGADDYSEIVTPTGTHLVRISLASLDERLGE
                     RFLRVHRSCLVNVDRISRVEPEGGGRMLVHMDSGEMIRTSRAGSRLLRDRVI"
     gene            complement(7772..8644)
                     /locus_tag="VF589_03765"
     CDS             complement(7772..8644)
                     /locus_tag="VF589_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256726.1"
                     /translation="MAIIKTRDGTEIFYKDWGEGPVVMLHHGWPLTADTWEDQSFFLA
                     SNGCRVIAHDRRGHGRSGQPWHGNDMDTYADDVADLVEALDLRDVTHIGHSTGGGQVA
                     RYIGRHGTDRVKKAVLVSAIPPTMLKTEANPGGLPIEAFDALRSGVATDRSQFYQDLA
                     IPFYGENRPGAKVSKGVKDAFWVQCMTVGIKASYDCIHEFSESVFTEDLKRFDVPTLI
                     IHGDDDQIVPFADSGPLSARLVKGARLSVYEGAPHGLPVTHRERFNAELLAFVMADGA
                     EAEPAPEREVEAVA"
     gene            complement(8754..9353)
                     /gene="wrbA"
                     /locus_tag="VF589_03770"
     CDS             complement(8754..9353)
                     /gene="wrbA"
                     /locus_tag="VF589_03770"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX8256727.1"
                     /translation="MVRILVLYYSSYGHIETLAYAMAGGARAAGAAVDVRRVPETVPE
                     AVARAAGYRLDQDAPIATIADLENYDAIIVGTGTRFGRMSSQMAAFLDQAGGLWARGA
                     LNGKVGAAFSSSATQHGGQETTLFSIITNLLHFGMVIVGLPYSHFGQMSVEEIVGGAP
                     YGATTVAGSDGSLVPTQIDIEGARHQGELVAKTARKLFG"
     gene            complement(9450..9938)
                     /locus_tag="VF589_03775"
     CDS             complement(9450..9938)
                     /locus_tag="VF589_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8256728.1"
                     /translation="MLSGTARSLPAMSFSMPAAYAARGPRLAAANAFGWSGLPQMWGP
                     PPLARAAASGSAADEFRFGRFRLLPAARTLLRDGRPVECGSRAFDLLHVLLLSRGKVV
                     AKEAIVRHVWPTTVVEESNLRFQMASLRKVLGDDRVLIKTIPGRGYLFASEFGGRDEE
                     AR"
     gene            complement(10046..11296)
                     /locus_tag="VF589_03780"
     CDS             complement(10046..11296)
                     /locus_tag="VF589_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8256729.1"
                     /translation="MFDWYRSLKKDGRRTFWACFGGYSLDAMDVQIFSFAMPVLIGLW
                     GMSNTDAGVLASATLIFSAIGGWGAGLLADRIGRVRTLQITIVWFAVFTFLSGLTTSF
                     EQLLVTRALQGLGFGGEWATGSVLIGEVVAARHRGKVGGIVQSGWGIGWGAAALLSTV
                     FFLTLPEEWAWRLLFFVGLAPAFGAFMVRRLVPESPLYLKSRASRAPRPGMLAIFSPP
                     YLATTVKGCLLALGLHGGYYAIITWLPTYLRTERHLSVLSTGGYLAVIIVGSLAGYWV
                     SAYLNDGWGRRKTFLLYAVGSLVIAFSYMLIPISNGLMLVLGFPLGFFASGIYSGIGP
                     LFTELYPTEVRGSGQGFCFNFGRGIAALFPILVGALSARVGLGTAIGLFASLAYGLVI
                     LMALILPETRGRALDEPGAAANGS"
     gene            11556..14357
                     /locus_tag="VF589_03785"
     CDS             11556..14357
                     /locus_tag="VF589_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8256730.1"
                     /translation="MSARALDILALLAGRSGEVVEKKELMALVWPDVTVDEGSLRFHM
                     NGLRKALGDGKDGARYIATIPGRGYCFVAPVLPAETGAAPAEPSTQSGDRPVLPPRLA
                     RMVGRAEAEEEIARSLGSNRFVTILGPGGIGKTTVAVSVGHRLVPDFAGGVKFVDLGS
                     TSDPALVPSMVASATGFVAHAADLVPSLIAFLREKRLLIILDSCEHVVDAVARLAERI
                     FTENPEVHILATSREALRVEGERVHPLPPLGSPPSHSDLAPDQVLSFPAVQLFVERVT
                     SSGVPFELTDADAPAVAEICRNLDGIPLAIELAAGRVNAYGVEGIAALLGDRFGQLAN
                     SRRTAVARHHTLSAALDWSYDLLAEPERTLLRRLSVFVGPFTLDAAQATAAFGELGEL
                     AALEGVGTLVSKSLIAADLAGAAPRYRLLDTTRGYARDKLIESGEARQVARRHALYYL
                     GALEQEGEPAGAAEPRCYLGNIRAALEWCFGEKGDPEVGTALAAAAAPLLVELSLLSE
                     CCGWAKRGIAALSASTRGTVREINLQAALGHSLMFTEGNSERVRECFTRGVTLARELR
                     EPFMELRLLGSLHLFNERMGNYHGAVEFAERSVVIARSIGHPAALAAAGSFLGLSQHL
                     VGNHLAARQLLEGALAQQAASPQARTIQFGFDYRNRSRITLARHHWLVGEADQAAALA
                     DRTIADAAGMRHPVTLCIALIWGITVSLWIGDASGSDEKIETFLAHAEKHSLNPYSAV
                     GCGYRGELCVQRGESEAGVALLERALETLHAARYELVTTSFMISLAQGLDKMGRHPAA
                     LETIDRALELVQANGDLLYMPEVLRTKGAILAGAGPANAGAAADCFRQSLDWARRQSA
                     LAWELRTATSYAALLARQGSREEAVERLAPIYSRFTEGLETADPRAARALLDLLAPGG
                     TAAAAVRPLPRRGNDAPAA"
     gene            14445..15374
                     /locus_tag="VF589_03790"
     CDS             14445..15374
                     /locus_tag="VF589_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8256731.1"
                     /translation="MKATAWKAALAAIAATATLAAAAAAQQGTPYDRINAAAASSEIT
                     VQPLRGKIVMLQGSGGNIGVLAGPDGMLMVDAGIAVSRAKIEQALRGLGPGKLRYVVT
                     THWHWDHADGNSWARPAGATVIAHAEAARRLADTIRVVEWEHTFTPIPEAARPNMLLT
                     KAKTIRFNGEDVLIRPYAAGHTDGDLSVYFPKADVLQTGDTWWNGQYPFVDYVGGGGI
                     DGAIEAANRNIAMAKGTTLVIPGHGPVGGRAELIEFRDMLVAVRTKVAALKRSGLSLE
                     QAVEAKPTAGFDDKWGASLIGGDLFTALVYRGV"
     gene            15398..16144
                     /locus_tag="VF589_03795"
     CDS             15398..16144
                     /locus_tag="VF589_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014277674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8256732.1"
                     /translation="MARLAGKIAVVTGGNSGIGLATAKRFAAEGAFVYIMGRRQAELD
                     AAVAEIDGEAAGVRGDIANLADLDSLYARVREEKGRIDILFANAGLGEFLPLGAITEE
                     HFDHTFAVNVKGTLFTVQKALPLMGQGGSIILTGSTAASTGSPSFSVYGASKAAIRSF
                     ARNWILDLKGTGPRVNILSPGPTETPGVRGLVTDEERAGFLNLLASQVPLGRVGRPEE
                     IAAAALFLASDDSSFVNGVELFADGGAAQV"
     gene            complement(16135..16515)
                     /locus_tag="VF589_03800"
     CDS             complement(16135..16515)
                     /locus_tag="VF589_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX8256733.1"
                     /translation="MELPTRELQEAIDARDPARIADFYAEDAMFLTPGRPAVEGRDGV
                     ERMMVEDLNDPGFGLDLTVQASKIAASGDLGYARGALRLSFTNPQTGEVASIAANYLQ
                     VFRKDGDGSWKVIEDISSPAAAQT"
     gene            complement(16632..>16950)
                     /locus_tag="VF589_03805"
     CDS             complement(16632..>16950)
                     /locus_tag="VF589_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8256734.1"
                     /translation="ITHDQALLDRPGNRDIQLDLFLDYGTNVPLYPQFQAFFREHRPP
                     TLIVWGKNDFIFPADGAGPYLRDLPDAEIHMLDTGHFALEDKLGVMAPLILDFLDREV
                     APR"
BASE COUNT         2737 a         5947 c         5725 g         2541 t
ORIGIN      
        1 gcggcagccc ttttccaacc gctacggcct cgcttatgcg acgctgcgcg gggaatggcg
       61 cggctatcgc ctgaccagcg ccttcagcct ggtcggccag gaccttgatg aatcgttcga
      121 cgcgaccctg cccgaaggac caaccgagct gttccggcag cgcaaccggc tcgaaatggt
      181 ctcgctggaa acccgcatcg cgcgcgaagc gcaaggcggc ggctggctgg ccgggctaag
      241 ccttctcgcc aaccggaccg agctcgaccg cgcggtcttg ccgcctggcc gcctgctcgc
      301 gctgccgggg gtcgccaacc ggctcgccga gcagaccctg ttcgccgagg cgacccggcc
      361 gctgtggagc ggcctcagcc tgacgctcgg cgcccgcctc agccatgtga gcttcaccgg
      421 ctccgcgacc gagccgcagg ggccggcgcc cgatgggata ggccgcatcg agggcgacga
      481 gtggcgcctg ctgccggcgg cggcgctcgg cgccagcctc gggagcgacc ttcgcctgta
      541 ccttcgctat caggagagct tccggccggg cggcgcgatc gtccgcacca acgccgccca
      601 gcggctggag agcgaccgca tccaggcctg ggaggccggc ctgcggttcg gccatggccg
      661 atcggcgcgg ctaagcggcg ccgtctcggc aagctattcg cgctggaaca acgtgcaggc
      721 cgacctcatc gacacgaccg gcctccccac caccgccaac ctcggcgacg gcgagatctg
      781 gacgctggac ggccggctcg aatggcgggc gacgccggcg cttcggctcg gcgtggccgc
      841 ggtggtgagc cgcagccggg tcagcaaccc cgatcccggc atcatcttca tcgaggacga
      901 accgctcccc aacgtgccgg acctcaccgc caccgccagc gtcgactatc gccgcccgct
      961 cggcgcctcg cgccagctcg agctctccgc ccgcgcccgc tatgtcggca aatcgacgct
     1021 cggcatcggc ccgatcctgg agcgcgccca aggcgattat gtcgacacgt ctcttgaagc
     1081 gcgaatcacc ggcggccgct ggatatggtt cgtcgccggc accaatctgc tgaacaccga
     1141 gggcaaccgg ttcgcgctcg gatcggtgct gaccctggtc agcgaagacc aggtgacgcc
     1201 gctgaggccg ctcacggtcc gcgtcggcgt ggagacgcgc ttctaagcgc gcgcggcgct
     1261 cacgcaggcg aactcgccct ggctcgatcg agcttgccgc ccatcaccca ccggccgaac
     1321 aggccgctgg ggcgggccag ctggcgggcc atgaagcgag tgaacagcat tgcgaatcaa
     1381 tcttcccgga tctcgtcccg atcgcggctg atcgcggccg gcgacggact agttgctcgc
     1441 cgggccttca atcaagatag ggcgcggcgt tggcggcgat ggtgcggaac tcggcttcgt
     1501 ccagcggaag ccgcgagaag gccgcccagg cctcggccgg cggcatcgcg ttcagtccgt
     1561 cgcggtcggt gccgaacagt atccggcgga cgccgagctc gcggattctc cgcgcgatca
     1621 gggcgagctg ctcgggggtc tcacgaccgg tcacgatagc ggcgacgtcg aaatagaggt
     1681 tcctcgcctg agggctgccc gccgcaatgg ctgcggcgaa gggggtgagc gcctcgtccg
     1741 gcccataggc cccgccgccg ccggccagat gcatgacctg cacggcgacg tcgggcgcca
     1801 tcggcaggat atcggcgagg aagatggcgg cgtcggcgct gccgtagcgg gggccgttcc
     1861 agagatggat cgcgatcgcc agcttggccg cgtcggcggc gcggaacacc ccggccagcc
     1921 gctcgacatg gtcgcgattg cgcaggtcga cgcccgaatt ccccaggtgg agcttgatcc
     1981 ccctcacctg ctcgatgcgg ccacagcggc ttatttccgc cagcgcatgg tccttgagcg
     2041 ggttgacgcc gcaaaaggcg acgaggcgcc cgggatattg cgcgacttgc cggacgatcc
     2101 agtcattttg cgcgctcacc aacgcggcct cgcgagggta tggcttcggg agcagcgggc
     2161 tggcgaagaa gtagcccggc gaatggacga cgccgcggcg aacgccggcg gcgtccattt
     2221 ccgccaccaa ttgctgcgca aacgtcgcgc gcggggtcgg cggggcccgc tcggaccagc
     2281 tttcggcggc gatcagccac ccggcgcctt cctttgtgag gctgaggtgg aaggtccgta
     2341 ccggccgcgc cgcctcgcct tcgccgcgca cgatgaggcc ggcgacgtgc gcgccctgcc
     2401 cgaccagcga atagcttacc ggcaccaggc gggaacctcg ctgggcaccg ccgagaaagg
     2461 cctggatcgc cgcccgcccc cgcacccagg tcggaccccg tatgtcgagg atgatcgcat
     2521 cgcgcgtgta aagatcgcgg gacggttgct cggtcctggc ccgcaacagg gaggcgagct
     2581 cttgtggaag cgcaaccggc ggcagcagcg gcggcaccag gatcgcggcc gcctccgggg
     2641 taagcaggtg caaatggtga tcgaccgcgg gagccgggat ccgagccgga gcggcgctcg
     2701 gccgggacgg cggcgcggtc ccgggctgtg ccgccgcaag cgcggcggaa gcgagcgaca
     2761 gcagagccgc cccgataggg aacctcatct cactaccccc tgtttttgct cgatgcaggc
     2821 gaaaagacga tgacaatccg ccagaagctg atagtctttg tcatatgata gagactgtca
     2881 gtttagatcg gcagcgtcaa caggacctgc cgctgcgcgc cagaaaaaag ctcgactcga
     2941 tgcggcgcgc ccaggcggcc gcgatccggc tgttcgccag gcggggcttt ggcggagtgt
     3001 cgatggagga ggtggcggcg gcgtccgatg tgtcgccggc ctcgctctac cgctatttcg
     3061 ggaccaagga aaatctgatc atctgggacg aggatgacga tccgctgttc gaccgcgtcg
     3121 cgcggcatct gcggaccatg ccgccgctgc aggcgttcgc cgccgccatc gtcgaggcgc
     3181 tgggcccgtc cttcgagacc ggacgggagg cccgaagcgg ccgcctgcgg ctgatcttcc
     3241 gggagcccgc cttgcgatcg gcgttccgcg aaaatggccg cctgctggcg cagggcctcg
     3301 ccagggtatt cgccagcggc agggcggcgg cggcgccctc gctgggcgat ctggcgctcg
     3361 cagcggccgc cgcggcgatc ctcgaggtcg ccgtcgagcg atgggcgacc ggcggcgccg
     3421 agacccgcct cgcccagata ataagcgagg ccttcgaatg cctcgcctag ccccccggtc
     3481 cggcgcgatg cggctgttgc ggcgggcggc ggcggtgctg ttgctgctgt tgctgctcgc
     3541 cgcccttctg ctggcggtcg agttcggcct gcgtcggccc tacacgcctg ccctccccga
     3601 cgagcccccg gcaaacgcga tcgcctcgct ggagcagctt ccgatccgcg gggttcccca
     3661 atcggtgctg atccgcggcc gcgaccgcac caagccgatc ctgcttttcc tgcacggcgg
     3721 gccgggaatg ccggtgatgt tcctcggcca cgccttccag cgcggccttg agcgcgactt
     3781 cgtggtggtc cactgggacc ggcgcggcgc cggccgctcc tacgattcct ggacgcccga
     3841 agcatcgtcg gttcgcgcga ccctcgacga tctgcacgcg ctgacggcgc agctgaagcg
     3901 gcggttccgg caggaccggg tctatctggt cggccacagc tggggcacct atctcggcct
     3961 gctcgcggtt cacgaacggc ccgacctgtt ctcggcctat atcggcaccg gtcagatggc
     4021 ggccgatccc gagcgcgtgc gggccgcgcg acaggctttc ttcgagcgcg aggcgcgggc
     4081 gaggggcgac gaggagatgc tcgggcggct ggctgccggg ggcgagatca ccgaagacga
     4141 tctgttccgc tacggcgccg agctgcgcgg cgcggaaagc ttctggccga tcctcatgct
     4201 cggcctcagg gcgccggaat atcggctgcg cgacgtcctc aacgtgccga aggggtcgga
     4261 gcgcctgctc cgcaccatgc gctacgacgt gatccagggt ccgctcgacc gcaacctcgt
     4321 cgagtttcgc gtcccgatct tcttcttcct gggccggcac gactggaacg agccctcgac
     4381 gctcgccgcc gattacctcc ggcgcatccg cgcccccgac aagggcctgg tgtggttcga
     4441 gcatgcggcc catttcccgt tcctcgagca gccggagcgg ttccacggcg cgatgtcggc
     4501 gctccaccgg cggctcaccg accggcgccc acccacgccg taagggggcg ggcccgacag
     4561 gatcgcgccg acctgggccg ctggccacgg gtgccgtcat gtgcgatgac gccagcgggg
     4621 atttcaactg cccatgatgc ttcgccggac gggttcggaa ccggcgtggc gtgttcgaat
     4681 gggggccggg atgacattca ctttgcaagg cgacacgctg gtcaatggca cgagcgccgg
     4741 cgaccaatac aattcctccc tcgcccgcct cggcgacggc agctatgtcg tcgtctggca
     4801 gaccggcgat gcggcgccca acgccctctt cggacagctt catgcatcag acggcacggc
     4861 gctggggggc gagtttcaga ttaacgccgg caccggcggg acctatgtcg aaccgcgggt
     4921 cgcggcgctt tcgggcggcg gtttcgccgt ggtctggtcg aattcctccg acctcagcta
     4981 ccgcctgttc gaagcggacg gcacgccttt gggcggcgat ctcatcctca cgacggccga
     5041 cgaaattggc ggctacgact ggcagatcga cgtcgccccg accgacgacg ggctcgtggt
     5101 gaccttcctg cggcccacct atggcgacat attctcggtc ggacgcgctt accggcaatg
     5161 acggctcgaa tggcctcgac gggcgcggcg gagcggaccg gctggagggc gccggcggca
     5221 atgaccgcta tttcgtcgat catgccggcg acgagatcgt cgaagcctcc gagggcggct
     5281 ccgacgcggt gttcagcagc gtcagccatc agcttgccga cgaggtcgaa gccctgcggc
     5341 tgaccggggc ctcggcgatc aacgggagcg gcaatggctc gggcaatcgc ctgttcggca
     5401 atgccgccgc caaccggctc gacggcggcg acaagagcga cggcctgttc ggcggcgccg
     5461 gcaacgacct gctgaccggc ggcgcgggcg ccgaccgctt ctacttcgac accgccctcg
     5521 acgcggccgc caatgtcgac gccatcagcg acttcgccga cggcgacagg atcttcctcg
     5581 cccgctccat cttcaccgaa atcgccgccg gcacgctcga tcccggcgcc ttttgcgcgg
     5641 gagcggccgc ggcggacgat agcgaccgca tcgtctacga cgtttcaacc ggtaacatct
     5701 tctacgatgc cgatggcagc ggttcgggcg cggccatgct gttcgcgacg gtggcggtgg
     5761 gcaccgaact cgaagccgcg gacttcatcg gctactgagc gggcgccgcg gaccgggctc
     5821 gcgctggcgg gcggcgcagg aatgccgcca cgtcgcgcgc gaagcgctcc ggcgtctcga
     5881 cgaacatgaa gtggccggcc tccggatatt ccagcaggcg tccacgcggt agctccttta
     5941 ccaagtcgcg ctgtggctcg atggccgtct gatagtccct gccgccggcg atgatcagca
     6001 ccggcgccac cacattggcg gcgcgcgaga accgatattg cagatagccc ttccggaaca
     6061 gcaagctggc cgcctcgccg gtggcgccaa ggccgccggc ggaatcgagc tcggtgaccc
     6121 gccgcgcggt cgccaggtcc gggaacaggt tgcggtgggc aaaggcggtg gccgcatcgc
     6181 cggtataagc gctcatcgtg ttgcagcggg ggacgacgcc ggcgcggatg cccgctgcgg
     6241 cccgcgcata ggcttcgggg tcctcgaccg cgagccgctc gcactgaagg tcgatcgaac
     6301 gcgtcaaatg cgccgccgcc gccgccagca ccacggcatg ggtttgccga gggtagcggg
     6361 cggcatattc gagcgcgagc tgggtgccga acgaatggcc gagcagcgcg atcttcggca
     6421 cgccgaggtg ccggcgaagc cgctcgacat cctcgaccat gatgtcgatc gaatagtaag
     6481 acggatcctg gggccggtcc gaacggccgc tcccgcgctg gtccagatag accaggcgct
     6541 gcgtcttttc gagctcgggt ccgccgaccg cctgaaaggt ctggctcccc tgccccggcc
     6601 cgccatggag gaagaccagc ggaatgccgc cggaccggcc cgcaacccga taccagagcc
     6661 gaacaccgtt cacgaccaca tgatgttcgc cggtggcgat tggccttggg gccgacgcgg
     6721 cgggcgtgct caaagaagca aggcaagccg ctgctgccag cacgaatagt ctcgtcatag
     6781 ctgtcctcgt gtcgcgtatg tcccatccgg agcaccctat cggatgcctc aggcggcctc
     6841 gcgaaaagca gcgaaacgac accgccgtgc ggtgaaagct ccgctgcctc gggctagatg
     6901 acgcggtcgc gcagcagccg cgagccggcg cggctggtgc ggatcatctc gccgctgtcc
     6961 atgtggacca gcatccgtcc gcccccctcc ggctcgaccc ggcttatccg gtcgacgttg
     7021 accagacacg agcggtggac gcggaggaac cgctccccca gccgctcgtc gagcgaggcc
     7081 aggctgatgc gcacgagatg ggtgccggtg ggcgtgacga tctcggaata atcgtccgcg
     7141 ccccggatga ggatgatgcg gtcggtgtcg agcggccgga gctcctcctc gtgtttgacg
     7201 aacagccgcg gcggctgcga gcttgcgctg ccgccttcgc gctccggggc cgggatcgga
     7261 ctccgctcgg cgagcatttc cgcgtgcacc gccacggtga tcgccgcata gacggtgagg
     7321 ccctggaata gctgccacgt cgccgccggg ccgaggaagg gccgcacgct gaactcgatg
     7381 gcattgcgcc cggcgaccgc gccgagcagg accatcagca gccagaacca ggctatcgtg
     7441 aagaccaacc ccagcgccag atgcccgacc attttggtga caagcggctt gtcggccatg
     7501 ctccgaatga cttgccggct ggcgagcccg agcaaagcga taggcacgac gttcgcgagc
     7561 gcattggcga tcacccgcga taaaggccaa ttgccggtaa gcgccagcac caggctgtag
     7621 gccacgaaca aaatggccag cgccgcgccg tagaaaaccc gccggcccgg gcggaaatcg
     7681 cggcgcagga aggcggggag aagcacccgc cgactatggc cgaatgccgg cggcgatcaa
     7741 cctcccccgc aggccgggtg cggctccccg cttatgcgac cgcctccacc tcccgctccg
     7801 gcgcaggctc cgcctcggca ccgtccgcca ttacgaaggc gagcagctcc gcgttgaagc
     7861 gttcgcggtg ggtcaccggc aatccgtgcg gggcgccctc atagacgctg agccgcgcgc
     7921 ccttcacaag ccgagcgctc agcggcccgg aatcggcgaa gggaacgatc tggtcgtcgt
     7981 cgccgtggat gatcagcgtc ggaacgtcga accgcttcaa gtcctcggtg aacacgcttt
     8041 ccgaaaattc gtggatacag tcataggagg ccttgatgcc gaccgtcatg cactgcaccc
     8101 agaaggcgtc cttgacgcct ttcgagactt tcgcgcccgg ccggttctcg ccatagaagg
     8161 ggatcgccag gtcctggtag aattgggacc ggtcagtggc gacgccggag cggagcgcgt
     8221 cgaacgcctc gatcggcagc ccgccgggat tggcctcggt cttcagcatg gtcggcggga
     8281 tcgccgagac cagcaccgcc ttcttcaccc gatcggtgcc gtgccggccg atataacggg
     8341 cgacctggcc gccgccggtc gagtggccga tatgggtgac gtcgcgaagg tcgagcgcct
     8401 cgaccagatc cgccacgtcg tcggcataag tgtccatgtc gttgccgtgc cagggctggc
     8461 ccgagcggcc gtggccgcgc cggtcgtggg cgatcacccg gcagccgttc gaagcgagga
     8521 agaagctctg gtcctcccag gtgtcggcgg tcagcggcca gccatggtgg agcatcacca
     8581 ccggcccctc gccccaatcc ttgtagaaga tctcggtgcc gtcgcgggtc ttgatgatag
     8641 ccatggcaaa tgccctttca gctgagatat tggtttctgg taagatcatc gcgtgccgcg
     8701 atcgaacgag atggcgttag atcgcgtgag aatggcgggc gagccgaggc ggatcagccg
     8761 aacaatttcc gtgcggtctt cgccaccaat tcgccctggt ggcgggcgcc ttcgatgtcg
     8821 atctgggtcg gcaccagcga cccgtccgat ccggcgacgg tggtcgcccc gtaaggcgct
     8881 ccgcccacta tctcctccac gctcatctgg ccgaaatggc tgtagggcag tcccacgatc
     8941 accatcccga aatggaggag gttggtgatg atcgagaaca aggtcgtctc ctgcccgcca
     9001 tgctgggtgg cgctggagct gaaggcggcg ccgaccttgc cgttgagcgc gccgcgggcc
     9061 cacaggccgc cggcctggtc gaggaacgcg gccatctgcg aggacatgcg gccgaagcgc
     9121 gtgccggtcc cgacgatgat cgcatcgtaa ttctcgaggt ccgcgatggt ggcgatcggc
     9181 gcgtcctggt cgagccggta accggccgcc cgcgccacag cttccggcac cgtctccggc
     9241 acccgcctga cgtcgaccgc ggctccggcc gcccgcgcgc cgcccgccat cgcataagcg
     9301 agcgtctcga tatggccgta ggaggaataa taaaggacga ggatcctgac catgacttcc
     9361 cccgtattgg gcccggattg gcagccgtgg cccgggtgtc atccttcgcc gcacccgggt
     9421 cacgtgagaa aatgcgagat gatcgcccgc tagcgcgctt cctcgtcgcg gccgccgaat
     9481 tcggaggcga acagatagcc ccgccccggg atcgtcttga tcagcaccct gtcgtcgccc
     9541 agcaccttgc gcaacgaggc catctggaag cgcagattgc tctcctcgac gacggtggtg
     9601 ggccagacgt ggcggacgat ggcctccttc gccaccactt tcccgcgcga gagcagcagc
     9661 acgtgcagaa ggtcgaaggc gcggctgccg cattcgaccg ggcgcccgtc gcgaagcagg
     9721 gtgcgcgccg ccggcagcag acggaacctg ccgaaccgga actcgtcggc ggccgagccg
     9781 ctcgccgctg cgcgcgccaa cggcggggga ccccacattt gcggcaggcc ggaccagccg
     9841 aacgcgttgg cggcggcgag gcgcggcccc cgggcggcat aggcggcggg catcgagaag
     9901 ctcatcgcag gcagtgatcg cgcggttcct gacagcatgt tccgctcctt cggcgagccg
     9961 cacaggcttg gcacctgttt cacggcatgg gagcgaggct aggaccggcg ggggggcttg
    10021 tcccgctatc gaccgttggc tgttgttatg agccgttagc ggcggcgccg ggctcgtcga
    10081 gcgcccggcc acgggtttcg ggcaatatca acgccatgag gatgacgagg ccgtaagcga
    10141 ggctggcgaa cagcccgatc gcggtgccga gccccacccg tgccgacagc gcgccgacga
    10201 gaatcgggaa caacgccgct atcccgcggc cgaagttgaa gcagaagccc tgccctgacc
    10261 cgcgcacctc ggtcggataa agctcggtga acagcgggcc gatgccgctg tagatgcccg
    10321 acgcgaagaa gccgagcggg aagccgagga ccagcatcag cccattcgag atcgggatca
    10381 gcatgtagct gaaggcgatg accagcgacc cgacggcgta gagcaggaag gtctttcgcc
    10441 ggccccaccc gtcgttcaga taggccgaga cccaatagcc cgccagcgaa ccgacgatga
    10501 tgacggcaag atagccgccc gtgctgagca ccgagaggtg gcgttcggtc cggagatagg
    10561 tcggaagcca ggtgatgatg gcgtaatagc cgccgtgcag gccgagcgcg agcaggcaac
    10621 ccttgacggt ggtggcgaga taaggcggcg agaagatcgc cagcatcccc ggccgcggcg
    10681 ctcggctggc ccgcgacttc agatagagcg gggattcggg cacgaggcgg cgcaccatga
    10741 acgcgccgaa cgcgggagcg aggccgacga agaacagcag ccgccacgcc cattcctccg
    10801 gcaaggtgag gaagaagacg gtcgaaagca gcgccgcggc gccccagccg atcccccagc
    10861 cgctttgcac gatgccgccc accttgccgc ggtgccgggc ggcgacgacc tcgccgatca
    10921 gcaccgaccc cgtcgcccat tcgcccccga accccaggcc ctgcagggcg cgggtgacga
    10981 gcaattgttc gaagctggtc gtcagccccg acaggaaggt gaaaaccgca aaccagacga
    11041 tcgtgatctg cagcgtgcgg acgcgtccga tccggtcggc cagcaggccg gcgccccatc
    11101 cgccgatcgc cgagaagatc aaagtcgcgc tcgccagcac gccggcatcg gtgttcgaca
    11161 tcccccacag gccgatcagc accggcatcg cgaatgagaa tatctgcacg tccattgcgt
    11221 ccagcgaata gccgccgaag cacgcccaga aggtgcgccg cccgtctttc ttcaagcttc
    11281 gataccagtc gaacatgctg ccccccgggg caaccgcaag gcggccgccc tccgccgaac
    11341 aggcgctcca atcctccgga actccccact gccccgatgc tagacgctga actgtaaggc
    11401 aggtagagta ggaaggtcgt gggtgaacaa ttcacggggg taagttcgca ctcatcggga
    11461 gattatccct gcggcttcac gacgccattt gctttggacc atttcgtctc aaccaggctg
    11521 aacgactgct cgaaagatcg ggcgtccggg tagccttgag cgcgcgcgcc ctcgacattc
    11581 tcgcactgct cgccggccgc tcgggcgaag tggtcgagaa gaaggagctg atggccctcg
    11641 tctggcccga cgtcaccgtc gacgagggca gcctgcgctt tcacatgaac ggccttcgca
    11701 aggctttggg cgacggcaag gacggcgcgc gctacatcgc cacgataccg ggccgcggct
    11761 attgcttcgt cgcgccggtc ctgcctgccg agaccggcgc cgcgccggcc gagccatcca
    11821 cccagtccgg cgaccgtcct gtcctgccgc cccggctggc gcgtatggtc ggccgcgccg
    11881 aggccgagga ggagatcgcg cggagcctcg gctccaaccg cttcgtcacc attctcggcc
    11941 ccggcgggat cggcaagacc accgtcgccg tctcggtggg ccaccgcctg gtgcccgact
    12001 tcgccggcgg cgtgaagttc gtcgacctcg gctccaccag cgatcccgcg ctggtcccca
    12061 gcatggtcgc ctccgccacc ggcttcgtcg cccatgccgc cgacctcgtc cccagcctga
    12121 tcgcgttcct tcgcgagaaa aggctgctga tcatcctcga cagctgcgaa catgtcgtcg
    12181 atgccgtggc gcgcctcgcc gagcgcatct tcacggaaaa tcccgaggtt cacatcctcg
    12241 ccaccagccg ggaagcgctc cgggtcgagg gcgagcgggt ccacccgctc ccgccgctgg
    12301 gaagcccgcc gagccattcc gacctcgcgc ccgaccaggt gctgtccttc ccggccgtgc
    12361 agctgttcgt ggagcgcgtc acatcgagcg gcgtcccgtt cgagctgacc gacgccgacg
    12421 ccccggccgt cgccgagatc tgccgcaacc tcgacggcat tccactggcg atcgaattgg
    12481 cggccggccg ggtcaatgcc tatggcgtcg agggcatcgc cgctttgctc ggcgaccgtt
    12541 tcggccagct cgccaacagc cgccgcaccg cggtcgcccg ccaccacacg ctgagcgcgg
    12601 cgctggactg gagctacgac ctgctcgccg agccggagcg gaccctgctt cgccgcctct
    12661 ccgtcttcgt cggccccttc acgctggacg cggcccaggc cacggcggcg ttcggcgagc
    12721 taggcgagct tgccgcgctg gaaggcgtcg gcacgctcgt gtccaaatcg ctcatcgcgg
    12781 ccgatcttgc cggcgccgcg ccgcgctacc ggctgctgga cacgacccgg ggctatgccc
    12841 gcgacaagct gatcgaaagc ggcgaagccc ggcaagtcgc gcgccgccat gcgctctact
    12901 atctcggcgc cctcgagcag gagggcgagc cggcgggcgc cgccgagccg cggtgctacc
    12961 tcggcaacat ccgcgcggcc ctggaatggt gcttcggcga gaagggagac cccgaagtgg
    13021 gtacggcgct cgcggccgcg gcagccccgt tgctggtcga gctctcgctg ctttcggaat
    13081 gctgcggctg ggccaaacgc ggcatcgcgg cgctgagcgc ctcgacccgc ggcaccgtgc
    13141 gggagatcaa tctacaggcg gcgctcggcc attcgctgat gttcaccgaa ggcaatagcg
    13201 agcgcgtccg cgaatgcttc acgcgcggcg tgacgctggc ccgcgagctg cgcgagccgt
    13261 tcatggaatt gcggctgctc ggcagcctgc acctcttcaa cgagcgcatg ggcaattatc
    13321 atggcgcggt cgagttcgcc gagcgcagcg tggtgatcgc gcgcagcatc ggccatcccg
    13381 ccgcgctggc ggcggcggga tcgttcctgg ggctgtccca gcacctcgtc ggcaaccatc
    13441 tggcggcgcg gcagcttctc gaaggggcgc tggcccagca agccgcttcg ccgcaggcca
    13501 ggacgatcca attcggcttc gactatcgca accgctcccg catcacgctc gcgcgccacc
    13561 actggctggt cggggaagcc gatcaggccg ccgctctggc cgaccgcacc atcgccgacg
    13621 ccgccgggat gcggcacccc gtcactctgt gcatcgcgct gatctggggc atcaccgtct
    13681 ccttgtggat cggcgacgct tcgggctccg acgaaaagat cgagaccttc ctcgcccatg
    13741 ccgagaagca ctcgctcaac ccctatagcg ccgtcggctg cggctaccgg ggcgagctct
    13801 gcgtgcagcg cggcgagagc gaagcgggcg tcgccctgct cgagcgcgcg ctcgagacgt
    13861 tgcacgccgc acgctacgag ctggtgacga cctcgttcat gatctcgctg gcgcaggggc
    13921 tggacaagat ggggcggcat ccggccgcgc tcgagacgat cgaccgggcg ctggagctcg
    13981 tccaggccaa tggcgacctt ctgtacatgc cggaagtgct tcgcaccaag ggcgcgatcc
    14041 tcgccggcgc cggaccggcc aatgccgggg ccgctgccga ctgcttccgc cagtccctcg
    14101 attgggcgag gcgccagtcg gcgctcgcgt gggagttgag gaccgcgacc agctatgcgg
    14161 ccctgctcgc gcgccaggga agccgcgaag aggcggtcga gcggctcgct ccgatctaca
    14221 gccgcttcac ggaaggcctg gagaccgccg accccagggc cgcccgcgcc ctcctcgatc
    14281 tgctggcgcc gggcggcacc gccgccgcgg ccgttcgccc gctcccgcga cgcggcaacg
    14341 acgcgcccgc cgcttagcaa gccttcacgc ccgctcacgc caactctctg cgcccggccc
    14401 gccgctgcct ctacaacacg ccgatgggag caagggagcg cgacgtgaaa gcaacggcct
    14461 ggaaagcggc gctggcggcg atcgctgcta ccgccacgct cgcggccgcc gccgccgcgc
    14521 agcagggcac gccctacgac cgcatcaatg ccgccgcggc aagctccgaa atcaccgtcc
    14581 agccactgcg cggcaaaatc gtcatgctgc agggctcggg cgggaatatc ggcgtgctgg
    14641 ccggtcccga cggaatgctg atggtggatg ccggcatcgc cgtctcgcgc gccaagatcg
    14701 agcaggcgct gcgcggcctc ggccccggca agctgcgcta cgtcgtcacc acccactggc
    14761 attgggacca tgccgacggc aacagctggg cccgccccgc cggcgccacc gtcatcgccc
    14821 acgcggaggc ggcgcggcgc ctcgccgaca cgatccgcgt ggtcgagtgg gagcatacct
    14881 tcacccccat acccgaagcc gcccggccca acatgctgct gacgaaggcc aagacgatcc
    14941 gcttcaacgg cgaggacgtg ctgatcaggc cctatgcggc cggccacacc gacggcgacc
    15001 tctccgtcta tttccccaag gccgacgtgc tccagaccgg cgacacctgg tggaacggcc
    15061 aatatccgtt cgtcgactat gtcggcggcg gcggcatcga cggagcgatc gaggcggcca
    15121 accggaacat cgccatggcc aagggcacca cgctcgtcat tcccggccac ggcccggtcg
    15181 gcggccgcgc cgagctcatt gagtttcgcg acatgctcgt cgccgtccgc accaaggtcg
    15241 cggcgctgaa gcgtagcggc ctgtccctcg agcaggcggt ggaggccaag cccaccgccg
    15301 ggttcgacga caaatggggc gcgtccctga tcgggggcga cctgttcacc gccctcgtct
    15361 atcgcggcgt ctgaagccgc catcaaagga gcaacaaatg gcccgactgg caggaaagat
    15421 cgcggtggtt accggcggca atagcggcat cggcctcgcc acggcgaagc gcttcgcagc
    15481 cgagggagcc ttcgtctaca tcatggggcg ccggcaggcg gagctcgacg cggcggtcgc
    15541 ggaaatagac ggcgaggccg ccggcgtgcg cggcgacatc gccaacctcg ccgatctcga
    15601 ctcgctttac gcccgggtgc gcgaggagaa gggccgaatc gacatcctgt tcgccaatgc
    15661 cggcctcggc gagttcctcc cgctcggcgc gatcaccgag gagcatttcg accatacctt
    15721 cgccgtcaac gtgaagggta ccttgttcac cgtccagaag gcgctgccgc tgatggggca
    15781 aggcgggtcg atcatcctca ccggctccac cgcggcaagc accggctcac cgagcttcag
    15841 cgtctacggc gcgtccaagg ccgcgatccg gagcttcgcc aggaactgga tcctcgacct
    15901 caagggcacc gggccacgcg tcaacatcct ctcgcccggc ccgaccgaga cgccaggcgt
    15961 ccgcggcctc gtcaccgacg aggaaagggc aggttttctc aacctgctcg cctcgcaagt
    16021 gccgctcggc cgcgtcggcc gccccgagga gatcgccgcg gccgccttgt tcctggcctc
    16081 ggacgacagc agcttcgtca acggcgtcga attgttcgcc gacggcggcg ccgctcaggt
    16141 ctgagccgcg gccggggacg atatgtcctc gatcaccttc cagcttccgt ccccgtcctt
    16201 gcggaacacc tgcaggtaat tggcggcgat gctcgcgacc tcgcccgtct gcgggttggt
    16261 gaagctgagc cgcaaggcgc cccgcgcata gcctaggtcg cccgacgcgg cgatcttgct
    16321 cgcctggacc gtgagatcga ggccgaagcc ggggtcgttc agatcctcaa ccatcatccg
    16381 ctcgacccca tcgcgcccct caaccgccgg tcgccccggc gtcaggaaca tcgcatcctc
    16441 ggcatagaaa tcggcgatcc gcgccggatc cctggcgtcg atcgcttcct gcagttcgcg
    16501 tgtcggcagt tccatgtccg tctcctgttg cggcaagaaa gactccgccg gggcaacccg
    16561 taacgggcgc ctcggcggag caggtcccgc gcgagggcgg cggagcgcgg ggaaggtccc
    16621 ggcggcccgc ctcagcgggg cgccacctcg cggtcgagga agtcgaggat cagcggcgcc
    16681 atcacgccaa gcttatcttc gagcgcgaaa tggccggtgt cgagcatgtg gatctcggcg
    16741 tccggcaggt cgcgcagata cggcccggcc ccgtcggcgg ggaagatgaa gtcgttcttc
    16801 ccccacacga tcagggtggg cggccgatgc tcgcggaaaa aggcctggaa ttgcgggtag
    16861 aggggaacgt tggtgccgta atcgaggaac agatcgagct ggatgtcgcg gttgccgggc
    16921 cggtcgagca aagcctggtc gtgggtgata
//
LOCUS       DASVRK010000107        12043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_18277, whole genome shotgun sequence.
ACCESSION   DASVRK010000107 DASVRK010000000
VERSION     DASVRK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18277"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VF589_03810"
     CDS             complement(<1..793)
                     /locus_tag="VF589_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEX8256735.1"
                     /translation="MKAGVRKFGTADRRLLPEGRLAGPMPWVIAIMMFLTVLAAAAGL
                     GLGSAARSLDADIGNRLTVQIVEANPDARAAQAARAVERLRALSGVTAVRRLDEAEME
                     RLLEPWLGEGGLEADIPVPAMIDVELSVDAYRRIDGIRAAVRDVAPSARVDDNAQWLA
                     PLAKLIEALQLLALGLVLLMIGATAATVVLAARAALDTHRSTIEVLHLMGATDIQVAR
                     LFQRRIALDALFGGMTGLLAAALVLLVIGNRVGALGSELLGSAGLP"
     gene            complement(790..1542)
                     /gene="ftsE"
                     /locus_tag="VF589_03815"
     CDS             complement(790..1542)
                     /gene="ftsE"
                     /locus_tag="VF589_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX8256736.1"
                     /translation="MHCKSSAGSAEAGLGAGTIVQFENVGLRYGTGAETLSDLSFSLR
                     EGGFYFLTGPSGAGKTSLLKLLYLAQRPSRGLIKLFGEDIVTMPRRRLPGFRRRIGVV
                     FQDFRLVSHLSAFDNVALPLRVAGVEDSDLHTPVTEMLSWVGLGHRSSARPATLSGGE
                     QQRIAIARAVIGRPELLIADEPTGNVDPEMASRLLHLFDALNKLGTTVVVATHDVHLI
                     ARVARSEMLRLDRGQIADPTGALRHPPRAAEG"
     gene            1647..2396
                     /locus_tag="VF589_03820"
     CDS             1647..2396
                     /locus_tag="VF589_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX8256737.1"
                     /translation="MILTCPACDTRYVVPDSAVGPTGRQVRCAACKNSWFQEPAGARA
                     AAAPLRAAAPQPQAETPPPSPAQTEHRARTEAVPAPPPPGPPPAAQPAPEPDGYVSDY
                     DEPDRPPRRNPARMYTMLAIGAALLMMLAVVVVSYYGIPGVGGRSQAAVNAGSALVIE
                     VTRKPERRLMESGNELLAVSGRISNPTDQVLPVPQIRAELRDDEDRVVYNWAISAPVP
                     RLEPKQSATFNSAEVDVPRDARKLNLRFASS"
     gene            complement(2377..3732)
                     /locus_tag="VF589_03825"
     CDS             complement(2377..3732)
                     /locus_tag="VF589_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8256738.1"
                     /translation="MRYNRRETIEEAGRIMRLAWPIMLTSLNWTLMHLIDVAVVGRFG
                     TFELGALAAGRTLTFITIVMGMAALSGVLVHVSRADGAGRLAETGDILRSGVALGLIN
                     GLVGLAVLLIWADELLAVIGVAAELRAPGAAVVRAMALAYPFQFVLAAASYFLEGISR
                     PRRVMAVNLAMLPVNALLAWAWVGGHFGLPALGAVGAALATSTVSAGGAAAILVYCWI
                     LPRAEERGVKDLSRASLRRAGAWLPRLAWFGSVPAVAAALELAGFSWLIALSTQLGVV
                     TAAAFQAMFSLHNLVFALAMGFGSAAGVRVGNAVGAGAIAEAWPRTLIAAGLSVLVLG
                     SLSLLFGLAATAVSSPFSDDPRVLALAAGLLALMAPFMLFDGLQYVFSYALRSLGEQV
                     WAGINGVIAFFFVTGGLGWWLVRHGWGPEGLVYAAAAGMVLCAVLQLARLLVVLRRTR
                     T"
     gene            3780..4877
                     /locus_tag="VF589_03830"
     CDS             3780..4877
                     /locus_tag="VF589_03830"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX8256739.1"
                     /translation="MRIGILTGGGDVPGLNPCIRSITLSADDLGWTVLGFRRGWEGVL
                     AIDPTDPGSIAANSMELTRERVRGIDRLGGTILHTSRLDPRSAARGDGTGHVLNVLER
                     LGIDAMVTLGGDGTLRFSAHLAGQGMAVISVPKTMDNDVFGTDYCIGFATAVSRSVAA
                     INALRTTVESHERIGVVELFGRRSGETALLAGFLAQVDRTIIAEVPADLDRLMPLLAE
                     DKASNPSGYAMCVISEGASITGGGAEDELSRPASERLEPGLGHRLGSEISRRIGSGTV
                     IQELAYLMRAGEPDAMDRMVGFAFGALAVQLLSRGERARMVALTGGNYCHVPIDTLLG
                     GTKSVDVDGLYDAVAYRARLMRVEGMPMFLY"
     gene            4970..5056
                     /locus_tag="VF589_03835"
     tRNA            4970..5056
                     /locus_tag="VF589_03835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5004..5006,aa:Leu,seq:gag)
     gene            complement(5117..8038)
                     /locus_tag="VF589_03840"
     CDS             complement(5117..8038)
                     /locus_tag="VF589_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256740.1"
                     /translation="MASSRLRGVACAGLVNILALVHASSAWAAEECGPVEPDGNVVCE
                     ASGSPYGDVDYNGADGIDLTIPSGAVVNGSVSVLGDGDIKVTSEEGSAINGQILPALD
                     IGSTSGTVTVTVAEVTGGLHGVAAVAGGDVTVSADKVDATIAIQAESVDGNVFVEARE
                     LHADGDGGAGVVAVTGAGSIQVNVNEVHSQNAFYNQGVAAFSESGDIVIEAGSIDIDG
                     FRSEGIRVETYSGDVSVKAGSIDITGGEIVGISVVNDTGHTAIDADSVVLKGSVARGI
                     FAASFAEIDVNAGYVETHGFIADGIQLLSTEGDIRATVGEIVTKGDWSAGISAETGGS
                     VSFDIGSVTTRGEYSDGVGAFAGGDVTLNVDNITVYGNGSSGVIVGTSEGDQTISVGS
                     VLVRGRQDAGSAIATSTFSGKVDIEVRDAAVSLHGRGIVTQSVEDTVAVTVAEDATVS
                     GKLGGIWVDSGAGSRIDIAGTVTSSEGYAIEVTGAGSEINIAATGTLPGNVLLSERRD
                     VLTNGGRFLLSGDSDFGGGNDRLVNGGVLGLAPAEAPVLVRLQNLERLDNQGVIDLAN
                     GRIGDRLVVSGELAGSAGGSIALDVSLKDRSADTIRVGSLAGTTELRLDYAEKLGGLG
                     LTGLTLLTADGDASGGELVLAEDSVTRGFVDFALDFNAGRGAWQLSGGLNDRGYRTAV
                     AGEGARDLWREGVRAWSHQLAGHRGEESEGLRFWTHGFVGKNEREAAAASRLGSRDLA
                     WSTDHNGVQLGVEKASDGFIIGLAGGYGSSETDFGAGEESRFDALNAAAYAGYSSGGW
                     FANAMLRADWLDVETDWSSVGLDADGEANMLGAAIELGHRADFGGMWLEPFVAVQWID
                     LDLPDLASEWGRVRYSGGTTVTGEAGVRLQAKDGWAGLPFEPSLLLALSVEGGDGDRA
                     ELEVAGDRVRIEEDGTRSYGRAGLGIAKDFGRFGLFAQGEARFGDVEGYNAVLGAKLS
                     F"
     gene            8237..10354
                     /locus_tag="VF589_03845"
     CDS             8237..10354
                     /locus_tag="VF589_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX8256741.1"
                     /translation="MASNASSPLKRTLITTLKIGIYTAVVAIVALVVAVGVAMSSLPS
                     FGELVRRDDLGQMIRVRAADGTVLVSLGPSFGEWLSYGEIPPIMRSAMIAVEDRRFRS
                     HPGVDPIGVARAVKVRWDRGQWAQGGSTITQQLARNIFLTNRRTFGRKVREAILALAL
                     ERRFSKDQILELYLNRVYFGGGAYGIDAASRRFFGHSADKLSLSEAAIIAGLVKAPSN
                     YSPTADAEAARGRADVVIRLMQENGDISPIEAASAEPAEVKLVPTPKQNSVRYFTDWV
                     LPQLDTLIEETVEPIDVWTTLDPRMQRAADQAINANTPRGAQGALVSLDRDGAVRAMV
                     GGRDYVTSLYNRATQATRQPGSSFKLFVYLSALEAGYTPADTVVDSPVTINGWTPRNS
                     TRSHSGAVTVREAFARSINTISAKLGQEVGFRTVADMAQRFGITTEVNTHPSMVLGTS
                     DVRLIDMTRAFASVSQKGVAVVPYGIRRVSTSDGELLYEHRTNESRVLVAPWVAAQMT
                     DLLQSAVLTGTGRAAQIGRPVAGKTGTTSSNKDGWFIGFSSGITTGVWMGRDDAKPIP
                     GLQGGRAPAGAFQDYMVQAVARRPAEQFDIKVALPDWEVEPDEEAWFGEPQEGGGMMV
                     DADGNPIQAQPGYSPRPDAPEPVGAPPPGPGAADERLDQDWLDRAVGRDRPPPPREMP
                     PPSRADVVRPDDDELASDGPDPM"
     gene            complement(10331..11035)
                     /locus_tag="VF589_03850"
     CDS             complement(10331..11035)
                     /locus_tag="VF589_03850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX8256742.1"
                     /translation="MSSELRFEGGGRSALLTVKPSGRARVMRLRVDQRTGAVVLTVPR
                     RVSQRKALQWAAGKRAWVEAVLAQLPDRQPIAPGSTVPLHGIPHIVEWAGDRPRLVRV
                     AEGRILVGGPVETIEPRLIRWLKQHAAEILDQETREFAAKAGVQVTRTGVGDPRSRWG
                     SCSSSGAIRYSWRLILAPDWVRRATVAHEVAHRVHMNHGPDFHALVAELLECDPAPAR
                     LWLRRNGAELHRIGPV"
     gene            complement(11025..11456)
                     /locus_tag="VF589_03855"
     CDS             complement(11025..11456)
                     /locus_tag="VF589_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEX8256743.1"
                     /translation="MTQAFWEKPLDQLSHAEWEALCDGCGKCCLHKVEDDDTGIIYGT
                     NVACKLLDRHSCRCSNYRERKSLVPDCVRLTAGKVDKLGWLPSTCAYRLRAAGLPLPG
                     WHYLVSGDREAVHAAGESVRGWTIAEGEAGDLEHHLIDREL"
     gene            complement(11453..>12043)
                     /locus_tag="VF589_03860"
     CDS             complement(11453..>12043)
                     /locus_tag="VF589_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX8256744.1"
                     /translation="MAGGVMNRMLLLAALLLASCSSGGGDSPPLQGATIGGPFTLTDQ
                     NGRQASDTDFAGKYRLVYFGFTYCPDVCPVDLQSIGQGLRRLEKSDPGLAAKVQPIFI
                     TVDPERDTPPVMKQYVAAFHPRLIGLTGSPQEIAAVAKQFGVYHMKGEVESAGGYSVD
                     HQRTALLFGPEGEPIAIVPHDQGPAAVAAELERWVK"
BASE COUNT         1948 a         4360 c         3987 g         1748 t
ORIGIN      
        1 ccggcagccc ggccgatccg agcagctccg agccgagcgc gccgacgcgg ttgccgatca
       61 ccaatagcac caaagcggcg gcgagaaggc cggtcatccc cccgaacaag gcgtcgagcg
      121 cgatccggcg ctggaacagc cgcgccacct ggatatcggt cgcgcccatc agatggagca
      181 cctcgatggt gctgcgatgg gtgtcgagcg cggcgcgggc ggccagcacc accgtcgccg
      241 cggtcgcgcc gatcatcagc agcaccaggc cgagcgcgag cagctgcagc gcctcgatca
      301 gcttggcgag cggcgccagc cactgcgcat tgtcgtcgac ccgcgccgag ggggcgacgt
      361 cgcgcaccgc cgcgcggatg ccgtcgatgc ggcgatacgc gtccacgctg agctccacgt
      421 cgatcatcgc cggcaccggg atgtcggctt cgaggccgcc ctcgcccagc cacggctcga
      481 gcagccgctc catctccgct tcgtcgagcc ggcgcaccgc cgtcacgccg gagagcgccc
      541 gcagccgctc gacggcacga gcagcctggg ccgcgcgcgc gtcgggattg gcttcgacga
      601 tctggaccgt gagccggttg ccgatgtcgg cgtcgaggct tcgggcggcg ctgcccagtc
      661 ccaagccggc ggcggcggcc agcacggtca ggaacatcat gatcgcgatc acccacggca
      721 tcggccccgc cagccgccct tccggcagca gccggcggtc ggcggtcccg aacttgcgaa
      781 cgccggcctt catccctcgg ccgcgcgcgg cggatggcgc agcgccccgg tcgggtcggc
      841 gatctggccg cggtcgagcc gcagcatttc cgaccgggcg acgcgggcga tgaggtggac
      901 gtcgtgggtc gcgaccacca cggtggtgcc gagcttgttc aaggcgtcga acagatggag
      961 cagccgcgag gccatttccg ggtcgacgtt gccggtcggc tcgtcggcga tcagcagctc
     1021 gggacggccg atcaccgcgc gggcgatggc gatgcgctgc tgctcgccgc ccgacagcgt
     1081 cgcgggccgg gcgctcgacc ggtggccgag cccgacccag gacagcatct cggtgacggg
     1141 cgtatgaagg tcgctgtcct cgacccccgc cacccgcagc ggcaaggcga cattgtcgaa
     1201 ggcgctgagg tgggagacca ggcggaaatc ctggaacacg accccgatcc tgcgccggaa
     1261 gccgggcaag cggcgccgcg gcatggtgac gatgtcctcg ccgaacaatt tgatcagccc
     1321 gcggctcggc cgctgcgcca gataaagcag cttcaggagc gacgtcttgc ccgcgcccga
     1381 cgggccggtc aggaagtaga aaccgccctc gcggagcgag aatgacaggt ccgacagcgt
     1441 ctcggcgcca gttccgtagc gcaagccaac attctcgaac tgaacgattg tgcccgcgcc
     1501 cagacccgcc tccgcactac ccgcgctgct tttgcaatgc atcgcatggc aggcaggctg
     1561 ccgtaaacac ggcatttgct tgcgcggcac ggccatgacg tgcttagatt cccgaacgta
     1621 gacgaacggg ggccagtaca gacgcgatga tcctcacctg tcccgcctgc gacacgcgtt
     1681 acgtcgtgcc cgacagcgcc gtcgggccga ccggtcgcca ggtccgatgc gcggcctgca
     1741 agaacagctg gttccaggaa cccgccgggg cccgcgcggc ggccgcacct ttgcgcgcgg
     1801 cagcgccgca gccgcaagcc gagacgccgc ccccatcccc ggcgcaaacg gagcatcgag
     1861 cccgcacgga agccgtcccg gccccgccgc cgccggggcc gccacccgcg gctcagcccg
     1921 cccccgagcc ggacggctat gtcagcgatt atgacgagcc cgaccgcccg ccccggcgca
     1981 acccggcgcg gatgtacacg atgctcgcca tcggcgccgc gctcctgatg atgctcgccg
     2041 tggtggtggt ctcgtattac ggaattcccg gcgtcggcgg gcgcagccag gcggcggtga
     2101 atgccggaag cgcgctggtg atcgaggtta cccgcaagcc cgagcggcgg ctgatggaaa
     2161 gcggcaacga gctgctggcc gtgtcggggc ggatcagcaa cccgaccgac caggtgctgc
     2221 ccgtgccgca aatccgcgcc gagcttcgcg acgacgagga tcgggtggtc tacaattggg
     2281 cgatctctgc gccggtcccg cgcctggagc cgaagcagag cgccaccttc aacagcgccg
     2341 aagtcgacgt gccgcgcgac gcacgcaagc tgaacctcag gttcgcgtcc tcctgagcac
     2401 caccagcagc cgcgcaagct gcagcacggc gcacagcacc atgccggccg ccgccgcata
     2461 gaccagccct tcggggcccc agccatggcg aaccagccac cagccgagcc cgccagtgac
     2521 gaagaagaag gcgatcacgc cgttgatccc ggcccagacc tgctcgccca gcgaccgcaa
     2581 ggcgtagctg aacacatatt ggaggccatc gaacagcatg aacggcgcca tcagcgccag
     2641 caaaccggcg gcgagggcga gcacgcgcgg atcgtccgaa aagggcgacg agaccgccgt
     2701 cgccgccagc ccgaacagca aggacagcga tccgagcacc agcaccgaaa ggccggcggc
     2761 gatgagcgtt cgcggccatg cctcggcgat cgcgccggcg ccgacggcat tgccgacccg
     2821 gaccccggcc gcggagccga aacccatcgc cagcgcgaac accagattgt ggagcgagaa
     2881 catcgcctgg aaggcggccg cggtgaccac gccgagctgg gtcgagagcg cgatcagcca
     2941 ggagaagccg gccagctcca gcgccgccgc caccgccgga accgatccga accaggcgag
     3001 gcgcggcagc caagcccccg cccggcgcag cgacgcccgc gaaaggtcct tgaccccccg
     3061 ttcctcggcg cggggcagga tccagcaata gaccaggatc gccgccgcgc ccccggccga
     3121 gacggtggag gtcgcgagcg cggccccgac cgccccaagc gccggcagcc cgaaatggcc
     3181 gccgacccag gcccaggcga gcaaagcgtt gaccggcagc atggcgaggt tgaccgccat
     3241 cacccggcgc ggccggctga tcccctcgag gaaataagaa gcggcggcga gcacgaactg
     3301 aaacgggtaa gccagcgcca tcgcccgcac caccgccgct cccggcgccc gcagctccgc
     3361 agcgacgccg atcaccgcca gcagctcgtc agcccagatc agcagcactg ccagcccaac
     3421 aaggccgttg atcagcccca aggcgacgcc cgagcggaga atgtccccgg tttcggccag
     3481 gcggccggcg ccgtccgccc gcgaaacatg gacgagcacg ccggaaagcg cggccatgcc
     3541 catgacgatg gtgatgaagg tgagcgtccg ccccgccgcc agcgcgccga gctcgaaggt
     3601 gccgaaccgg cccaccaccg ccacatcgat gaggtgcatc agcgtccagt tgaggctggt
     3661 cagcatgatc ggccaggcga gccgcatgat gcgcccggct tcctcgattg tttcccggcg
     3721 gttgtagcgc atggccgccg ccgatagacg agcggggtcg gcgcgcaagg gagtaggcga
     3781 tgcggatcgg aatcctgacc ggcggcggcg acgtgccggg actcaatccc tgcatccgct
     3841 cgatcaccct gtccgccgac gatctcggct ggaccgtact cggctttcgc cgcggctggg
     3901 agggcgtgct cgccatcgat ccgaccgatc ctggcagcat cgccgccaac agcatggaac
     3961 tgacccgcga gcgagtccgc ggcatcgacc ggctcggcgg caccatcctc cacacctccc
     4021 gcctcgatcc gcgcagcgcc gcccgcggcg acggcaccgg tcacgtcctc aacgtgctcg
     4081 agcggctcgg cattgacgcc atggtgacgc tcgggggcga cggcaccttg cgcttttcag
     4141 cccatctcgc cggccagggc atggccgtca tctccgtgcc caagacgatg gacaatgacg
     4201 tgttcggcac cgattattgc atcggcttcg ccaccgcggt cagccgttcc gtcgccgcga
     4261 tcaatgcgct ccgcaccacc gtcgagagcc atgaacggat cggcgtggtc gagctgttcg
     4321 gccgccgcag cggcgaaacc gccctgctcg ccggcttcct cgcccaggtc gaccgcacca
     4381 tcatcgccga agtgccggcc gacctcgacc ggctgatgcc gctgctcgcc gaggacaagg
     4441 cgtccaatcc gtccggctac gccatgtgcg tcatttccga aggcgcctcg atcacgggcg
     4501 ggggcgcgga agacgagctg tcgcggcccg ccagcgaacg ccttgagccc ggcctcgggc
     4561 accggctcgg ctcggagatc agccgccgga tcggctccgg aaccgtcatc caggagctcg
     4621 cttatctgat gcgcgcgggc gagcccgacg cgatggaccg gatggtcggc ttcgcgttcg
     4681 gcgcgcttgc cgtgcaattg ctgagccgcg gcgagcgcgc ccgcatggtg gcgctgaccg
     4741 gcggcaatta ttgccatgtc ccgatcgata ccctgctcgg cggcaccaaa tcggtcgacg
     4801 tcgacggcct ctacgacgcc gtcgcctacc gcgcccggct gatgcgcgtc gaaggcatgc
     4861 cgatgttcct ctactgaggc cgccgaaccg gattgcgcgc gtccggccgc tttgctagag
     4921 gcccgatcct gccagccacc ggaagccttc cgtgtggact catcaaagtg cggtcgtggc
     4981 ggaactggta gacgcgcaac gttgaggtcg ttgtgggcga aagcccgtgg aagttcgagt
     5041 cttctcgacc gcaccagcag aaatggggac atgcccggcc ggcatgtccc ctttttcttg
     5101 cgcgcgccgg atcgcctcag aagctcagct tagcccccag cacggcatta tagccctcga
     5161 cgtcgccgaa ccgggcttcg ccctgcgcga acagcccgaa gcggccgaaa tccttggcga
     5221 tgccgagccc ggcgcggccg tagctgcggg tgccgtcctc ctcgatccgg acgcggtcgc
     5281 ccgcgacttc gagctcggcg cggtcgccat cgccgccctc caccgacagc gccagcagca
     5341 gcgagggttc gaacggcagg cccgcccagc cgtccttggc ctggagccgg acgcccgctt
     5401 cacccgtgac cgtggtgccg cccgaatagc ggacgcggcc ccattcgctg gcgagatcgg
     5461 gaaggtcgag gtcgatccac tgcaccgcga cgaacggctc gagccacatg ccgccgaaat
     5521 cggcgcggtg accgagctcg atcgcggcgc cgagcatgtt cgcctcgccg tccgcgtcga
     5581 gcccaaccga gctccaatcg gtctccacat cgagccagtc ggcccgcagc atcgcattgg
     5641 caaaccagcc gccggaggaa tagccggcat aagcggcggc gttgagcgcg tcgaagcggc
     5701 tctcctcgcc cgctccaaag tcggtttcgg acgatccgta gccgccggcg agaccgatga
     5761 tgaagccgtc actcgccttt tccacgccga gctggacgcc gttgtgatcg gtgctccagg
     5821 cgaggtcgcg cgagccgagg cgcgaggcgg ccgcggcctc gcgctcattc ttgccgacaa
     5881 agccgtgggt ccagaagcgc aggccctcgc tctcctcgcc gcgatggccg gcgagctggt
     5941 gcgaccaggc gcggacgccc tcccgccaca ggtcgcgagc gccttcgccg gccacggccg
     6001 tgcggtagcc gcggtcgttg aggccgccgc tgagctgcca cgcgccgcgg ccggcgttga
     6061 agtcgagcgc gaagtcgacg aagccgcgcg tgacgctatc ctcggccagc acgagctcgc
     6121 cgccgctggc gtcgccatcc gcggtcaaaa gcgtgaggcc ggtgaggccg aggccgccca
     6181 gcttctcggc ataatcgagg cgcagctcgg tcgtgccggc cagactgccg acgcgaatcg
     6241 tgtcggcgct ccgatccttc agcgagacgt ctagcgcgat gcttccgccg gccgatccgg
     6301 cgagttcgcc cgagacgacc aaacgatcgc cgatgcggcc attggcgagg tcgatcacgc
     6361 cttggttgtc gagccgctcg aggttctgga gcctgaccag caccggcgcc tcggccggcg
     6421 ccagcccgag cacgccgcca ttgaccaaac ggtcgttgcc gccgccgaaa tcgctgtcgc
     6481 cggacagcag gaaccgaccg ccattggtca gcacgtccct ccgctccgac agcaggacgt
     6541 tgccgggcaa ggtgccggtc gcggcgatgt taatctcgga gcccgcgccg gtgacctcga
     6601 tcgcgtagcc ttcggagctg gtcacggtgc cggcgatatc gatacggcta cccgcccccg
     6661 aatccaccca gatgccgccg agcttgcccg agacggtcgc gtcctccgct accgtcacgg
     6721 cgaccgtgtc ctcgacgctc tgggtgacga tgccgcgccc gtgcagcgag accgcggcgt
     6781 cccgcacttc gatgtcgacc ttgccgctga aagtgctggt ggcgatggcg ctgccggcgt
     6841 cctgcctgcc gcgcaccagc accgagccga cgctgatggt ctggtcgccc tcgctggtgc
     6901 ccacgatcac gccgctggag ccgttgccgt agaccgtgat gttgtcgacg ttgagggtga
     6961 cgtcgccgcc ggcgaacgcc ccgacgccgt ccgaatattc gccgcgcgtg gtgaccgagc
     7021 cgatgtcgaa gctgaccgac ccgccggtct cggcgcttat gcccgccgac cagtcgccct
     7081 tggtgacgat ctcgccgacc gtcgcgcgga tgtcgccttc tgtggaaagg agctgtattc
     7141 cgtcggcgat gaatccgtgg gtttccacgt agcccgcatt gacgtcgatc tccgcgaagc
     7201 tggcagcgaa gatgccgcgc gcgacgctgc ccttcaggac gacgctgtcc gcgtcgatcg
     7261 cggtgtggcc ggtgtcgttc acgaccgaga tgccgacgat ctccccgccg gtgatgtcga
     7321 tgctgccggc cttgaccgaa acgtcgcccg aataggtctc gacgcggatg ccctcgctgc
     7381 ggaagccgtc gatgtcgatg ctgcccgcct cgatgacgat atcgccgctc tcgctgaagg
     7441 ccgccacgcc ctggttgtag aacgcgttct gcgagtggac ctcgttgacg ttcacctgga
     7501 tggaaccggc gccggtcacc gccaccacac ccgccccgcc gtcgccatcg gcatggagct
     7561 cgcgtgcctc gacaaagaca ttgccgtcga cgctctcggc ctggatggca atggtggcgt
     7621 cgaccttgtc ggcggagacc gtgacgtcgc cgccggccac cgcggcgacg ccgtgaaggc
     7681 cgccggtcac ctcggcgacc gtgacggtga cggtgccgct ggtcgagccg atgtcgagtg
     7741 ccggcaggat ctggccattg atcgcgctgc cctcctcgga ggtcaccttg atgtcgccgt
     7801 cgccgagcac gctcaccgag ccgtttacca ccgcgccgct cgggatggtg agatcgatgc
     7861 cgtcggcgcc gttatagtcc acgtccccat aaggggagcc gctcgcctcg cagacgacat
     7921 tgccgtcggg ctcgacgggc ccgcattcct cggccgccca ggcgctggag gcgtgcacca
     7981 gcgccaggat gttgaccagg ccggcgcaag cgacgccgcg cagcctgctc gatgccacgt
     8041 ctcttccgat cacgatcgct cctcaggata tgcccttgcc ccaggcgcgc tgccatggcg
     8101 cggtcgccgc gtccttccac ggcctctcga cgagcctgac attttcttcg tcgaacgtgc
     8161 gccgcgggac ccccggaaaa ggcgcggccg ccttgttctt gtggcattca gcgagtggcc
     8221 ctatcgctaa catgcgatgg ccagtaacgc ttcctctccg ctcaagcgga ccctgatcac
     8281 taccctcaag atcggcatct ataccgcggt cgtggcgatc gtggcgctgg tggtcgcggt
     8341 cggggtggcg atgagctcgc tgcccagctt cggcgagctc gtccgccgcg acgatctcgg
     8401 ccagatgatc cgcgtccgcg ccgccgacgg caccgtgctc gtgtcgctcg ggccgagctt
     8461 tggcgaatgg ctgagctacg gcgaaatccc gccgatcatg cgatcggcga tgatcgccgt
     8521 cgaggatcgc cgcttccgct cgcatccggg ggtcgacccg atcggcgtcg cccgcgcggt
     8581 gaaggtgcgc tgggaccgcg gacagtgggc gcagggcggc tcgaccatca cccagcagct
     8641 cgcccgcaac atcttcctca ccaaccgccg caccttcggc cgcaaggtgc gcgaagcgat
     8701 cctcgcgctg gcgctcgagc gccgcttcag caaggaccag atcctcgagc tttacctcaa
     8761 ccgcgtctat ttcggcggcg gcgcctatgg catcgacgcg gcatcgcgcc gcttcttcgg
     8821 tcacagcgcc gacaaattga gcctgtcgga agcggcgatc atcgccggcc tggtcaaggc
     8881 gccgtccaat tactcgccga ccgccgacgc cgaggctgcc cgcggccgcg ccgacgtcgt
     8941 catccgtctg atgcaggaaa atggcgacat atcgcccatc gaggccgctt cggccgagcc
     9001 cgccgaggtc aaactggtgc cgacgcccaa gcagaacagc gtccgctact tcaccgactg
     9061 ggtgctgccg cagctcgaca ctctcattga ggagacggtc gagccgatcg acgtctggac
     9121 cacgctagac ccgcgcatgc agcgcgccgc ggaccaggcg atcaatgcca acacgccgcg
     9181 cggcgcccag ggcgccctgg tgtcgctcga ccgggacggc gcggtccgag ccatggtcgg
     9241 agggcgcgac tatgtcacct cgctctacaa ccgggccacc caggccaccc gccagccggg
     9301 ctcctcgttc aagctgttcg tctacctctc ggcgctcgag gcgggctaca cgccggcgga
     9361 tacggtggtg gattcgccgg tcaccatcaa cggttggacg ccgcgcaaca gcaccagaag
     9421 ccattccggc gcggtcaccg tccgcgaggc gttcgcgcgc tcgatcaaca ccatctccgc
     9481 caagctcggc caggaggtcg gcttccgcac ggtcgccgac atggcccagc gcttcggcat
     9541 cacgaccgag gtcaacactc acccctcgat ggtgctcggc acgtccgacg tccggctgat
     9601 cgacatgacc cgggcgttcg cgtcggtctc gcagaaaggc gtcgcggtcg tgccctatgg
     9661 catccgccgc gtgagcacct ccgacggcga attgctttac gagcaccgca ccaatgaatc
     9721 gcgggtgctg gtggcgccat gggtggccgc gcagatgacc gacctgctcc agtcggcagt
     9781 gctgaccggg accgggcgcg cggcccagat cggccgcccg gtcgccggca agacgggcac
     9841 gaccagttcc aacaaggatg gctggttcat cggcttttcg agcggcatca cgaccggcgt
     9901 ctggatgggc cgcgacgacg ccaagccgat cccgggcctg caggggggcc gcgcgcccgc
     9961 cggggcgttc caggattata tggtccaggc agtcgcgcgc cggccggccg agcagttcga
    10021 catcaaggtg gcgctccccg actgggaggt ggagccggat gaggaggcct ggttcggcga
    10081 gccgcaggag ggcggcggta tgatggtcga tgccgacggc aatccgatcc aggctcaacc
    10141 cggctatagc ccgcgtccgg atgctcccga gccagtcggc gcaccgccgc ccggtcccgg
    10201 cgccgccgac gaacggctcg atcaggattg gctggaccgc gccgtcggcc gcgatcggcc
    10261 gccgccgccg cgcgagatgc cgccgccttc cagagcggac gtggtgcggc cggacgacga
    10321 cgagctggcc tcagacgggc ccgatccgat gtagttcggc cccgttccgg cgcagccaca
    10381 gccgcgcggg cgcgggatcg cattcaagca gctccgccac cagcgcgtgg aaatccgggc
    10441 cgtggttcat atgcacgcgg tgagcgacct cgtgcgccac cgtcgcccgg cggacccagt
    10501 cgggggcgag gatcagccgc cagctgtagc gtatggctcc ggaagatgag cagctccccc
    10561 agcgggagcg cgggtcgccg acccccgtcc tggtgacttg caccccggcc ttggcggcga
    10621 actcgcgcgt ctcctgatcg aggatctcgg ccgcatgctg cttgagccag cggatcagcc
    10681 tcggctcgat ggtctcgaca ggcccgccga caaggatccg cccttccgcc acccggacca
    10741 gccgcggcct gtcgccggcc cattcgacga tgtgcgggat gccgtgaagc ggcacggtgc
    10801 tcccgggggc gatcggctgc cgatcgggga gctgcgccag caccgcctcg acccaggcgc
    10861 gcttgccggc cgcccattgc agcgccttgc gctgggacac gcgcctcggg accgtcagca
    10921 cgaccgcgcc ggttcgctgg tcgacgcgca ggcgcatcac gcgcgcgcgt cccgatggct
    10981 tcaccgtcag gagcgcgctg cggccgccgc cttcgaacct gagctcagag ctcacgatca
    11041 atcagatgat gctcgagatc gccggcctcg ccttctgcta tcgtccagcc gcgcaccgat
    11101 tcgcccgcgg catggaccgc ctcgcggtcg ccgctcacca gataatgcca gccgggaagc
    11161 ggcaatcccg ccgcgcgcag ccggtatgca caggtcgacg gcagccagcc gagcttgtcg
    11221 accttgcccg ccgtcagccg aacgcaatcc ggaaccaggg atttgcgctc gcgataattg
    11281 gagcagcggc agctatgccg gtcgagcagt ttgcacgcca cgttggtgcc gtagatgatg
    11341 ccggtatcgt catcctcgac cttgtgcagg cagcattttc cgcagccgtc gcaaagcgcc
    11401 tcccattcgg cgtgactaag ctggtcgagc ggcttttccc aaaaggcttg cgtcatttca
    11461 cccaacgttc gagctcggcc gcgaccgcag ccggtccctg atcatgcggc acaatggcga
    11521 tcggctcccc ttcggggccg aacagcaagg ccgtgcgctg gtgatcgaca gaataaccgc
    11581 cggcgctctc aacctccccc ttcatatggt agacgccgaa ctgttttgcg accgccgcta
    11641 tctcctgcgg gctgccggtg aggccgatca ggcgtgggtg gaatgcggcg acatattgct
    11701 tcatgaccgg gggagtatcc cgctcgggat cgacggtgat gaagatcggc tgcaccttgg
    11761 cggcaaggcc cggatcgctc ttttccagcc ggcgcaggcc ctgcccgatc gactgcaggt
    11821 cgacggggca gacgtcgggg caataagtga agccgaaata gacgagccgg tatttgcccg
    11881 cgaaatcggt gtcgctcgcc tgccggccat tctggtcggt gagcgtgaaa ggcccgccga
    11941 tcgtcgcgcc ctgcaacggg ggagaatccc ccccgcccga cgagcatgaa gcgagcaaca
    12001 gggctgcgag caggagcatt ctgttcatga caccgccagc cat
//
LOCUS       DASVRK010000108         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_454932, whole genome shotgun sequence.
ACCESSION   DASVRK010000108 DASVRK010000000
VERSION     DASVRK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454932"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF589_03865"
     CDS             complement(<1..193)
                     /locus_tag="VF589_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256745.1"
                     /translation="MYRFTLPLVALVAACSPLRTFDALVPKDRGVERVARDLAFAEGP
                     RGRLDLYAPADPRAGPLPVI"
     gene            296..463
                     /locus_tag="VF589_03870"
     CDS             296..463
                     /locus_tag="VF589_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256746.1"
                     /translation="MGSNSGGGKSSNNTGNPTLEEHERANKGTSGAAIHGEKKVNKGT
                     VGKHDGPGKSP"
     gene            complement(596..934)
                     /locus_tag="VF589_03875"
     CDS             complement(596..934)
                     /locus_tag="VF589_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEX8256747.1"
                     /translation="MINRFNRLALAAAALAALVPAAASADIGSGDTYVARVESSDLNL
                     SSKAGQATLANRIDAAADKVCGYTGTVSLRDFTASAACRLSFEKAALGQANTLLAANG
                     RGASFAGASR"
     gene            1336..>4016
                     /locus_tag="VF589_03880"
     CDS             1336..>4016
                     /locus_tag="VF589_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256748.1"
                     /translation="MAQLTGTSGDDTLFGIGEADSINGLEGSDTIVGGAGNDTIESDH
                     WIEFYIPDPSRPFNVITLHHYDDEVADHIDGGNGIDWARLDFHLNSSAVSLDFTNPAI
                     QQTTAYGTTIVNVERIHFVGGSGGDTVTAGAYADTLSGGGGADILNGAGGDDHIAARF
                     GDGADNLNGGDGVDLATLDFRFANGGGTYDFSNPAAVVALVEGTQIVNVERFDFQLSE
                     HGEVVTGGGYDDKFNGNGGDDQLRGGGGNDLLSGSSGTDLLSGEGGDDTIEAYDDAGA
                     DTIDGGDGIDLLKLHLGFRTTAVSYSFADPFATTTLADGTTVIGVERVAIVGGSADDF
                     FAGGGYDDSIDGNGGSDLVFGGGGNDFIVGDGGAFADERGNDTLDGGAGDDRISGMGG
                     DDYLVGGDDDDSLAGGHGTDELYGQAGDDSLEAGFNDGADIVDGGDGIDLVIITASGD
                     FGPSGDLIYNFSNPGAVYAIEGGATISNVERVDMTGASGNDQLTGGAYADRFLGFSGN
                     DTLRGGGGNDSLNGMTGQDFLFGDAGEDDFTYILTAAGAADRDAIDGGADIDFIFLNH
                     TLPTGPANYDFSNSAATITRPDGTTIVNVERIKYWGGTGVDTIGGGAYDDEILGAGGN
                     DSILGRDGSDRLEGGSGEDFLNGDQGNDKVYGGEGGDTLFGGGYTGPENDELYGDAGT
                     DFLVGSGGDDILDGGKDSDVDKLHGGVGNDTYHMYAGDIFATAAESGQGQEAEIDGFG
                     GGVDHVISHASSYTLLYSFENLTLAANAVSGFGSEDDNEILGNDDNNLIGGNGGNDKI
                     FGGTGRDTLSGGAGFDFFYLADGDAEAGESFNGGADSDRIDTASARLDLSGVTINELE
                     AIQTVRGAGTAFIVDTAAHAVLVNGAGGYDSLTVNDET"
BASE COUNT          738 a         1296 c         1410 g          572 t
ORIGIN      
        1 cgatcaccgg cagcggcccg gcgcgcgggt cggcgggggc gtaaaggtcg agccggccgc
       61 gcgggccttc ggcgaaggcg aggtcgcggg cgacgcgctc gacgccgcgg tccttgggca
      121 ccagcgcatc gaaggtcctg agcggggagc aggcggcgac cagcgcgacc aagggaaggg
      181 tgaagcggta catagatgcg gcatatgggt gcgcgccgcc gcgccgccac ccccgcggcc
      241 ggaaacaagt cgcccgcgcc ggggttcagc caccgacagc caatggagag gatcaatggg
      301 cagcaacagc ggcggtggca agagcagcaa caataccggc aacccgacgc tcgaggagca
      361 tgagcgggcc aacaagggca ccagcggcgc cgccattcac ggcgagaaga aggtcaacaa
      421 gggcaccgtc ggcaagcatg acgggccggg caagtcgccc tgacggggta gcgcggagga
      481 cgctcccgca cgcgccggtc gcctggaatc acgaggctgc ccggctaggc gggacacgcc
      541 aagggcggcg aggtcgcccc cgccgcccgg tcgcggcgcc agtcacctgg cggggttagc
      601 ggctggcgcc ggcgaagctg gcgccgcggc cgttggcggc gagcagggtg ttggcctggc
      661 cgagcgcggc cttctcgaag ctcaagcggc aggcggcgga cgcggtgaag tcgcgcagcg
      721 agacggtgcc ggtatagccg cataccttgt cggcggcggc gtcgatgcgg ttggcgaggg
      781 tggcctggcc ggccttgctc gacaggttga ggtcgctcga ttcgacgcgg gcgacatagg
      841 tgtcgccgga gccgatatcg gccgaagcgg cggcggggac caaagcggcc agagcggccg
      901 cggcgagggc gaggcgattg aagcggttga tcatggtcgt tcctgtcctg aaaatcacgt
      961 cgtgggacga cagggagccg aaccggccgg ggggcatgag cgggcccaac tccctgtcgc
     1021 tggagccctt ttacggtggg ccgagaggcg cggcgcggcc gcttcgacga tcatcggctt
     1081 tccttcggcg aagcttgccg gcgcccgacg aaaggccgcc cggcccggac gaaaggctcg
     1141 gcggcatgcc tgtgcggacg ccaggccctg cggccgtcgc tgagagccgc cgcctgctgc
     1201 ccgagtccgg gacaagctga aaaatccagt tgcctcaaac aggtagctcg cgggcgcaaa
     1261 atagagtaga agccgcgcct tgagatacgg gggagagtcg cggggtgcgg ctgccaccgg
     1321 tgggggaggc ggcgaatggc ccagctaaca ggcaccagcg gcgacgacac gctgttcggg
     1381 atcggcgagg ccgactcgat caacgggctg gagggctccg acacgattgt cggcggcgcg
     1441 gggaacgaca cgatcgagtc cgaccattgg atcgaattct acatccccga cccgagcagg
     1501 cctttcaacg tcatcaccct ccaccattat gacgatgagg tcgccgacca tatcgacggc
     1561 ggcaacggca tcgattgggc gcggctcgat ttccacctca attccagcgc cgtcagcctc
     1621 gacttcacca accccgccat ccagcagacc accgcctacg gcaccaccat tgtcaacgtc
     1681 gaacggatcc acttcgtcgg cggctcgggc ggcgacaccg tcaccgccgg ggcctatgcc
     1741 gacacgctga gcggcggcgg cggcgccgac atcctcaacg gcgcgggcgg cgacgaccac
     1801 atcgccgcgc gtttcggcga cggcgccgac aatttaaacg gcggcgacgg cgtcgatctc
     1861 gcgaccctcg atttccgttt cgccaacggc ggcggcacct acgatttctc caatcccgcc
     1921 gcggtggtgg ccttggtcga agggactcag atcgtcaacg tcgagcgctt cgacttccaa
     1981 ctcagcgagc atggcgaggt cgtcaccggc ggcggttacg acgacaagtt caacgggaat
     2041 ggcggcgacg accaactgcg cggcggcggc gggaacgacc ttctgagcgg cagttccggc
     2101 accgatttgc tttccgggga gggcggcgac gacacgatcg aggcttatga cgacgcaggc
     2161 gcggacacga tcgacggcgg cgatggcatc gatctgctca agctccatct cggcttccgg
     2221 acgaccgccg tcagttacag cttcgccgat ccgttcgcga caacgacgct ggccgacggc
     2281 accaccgtga tcggcgttga gcgggtcgcc atagttggcg gcagcgcgga cgacttcttt
     2341 gctggcggcg gttacgacga ttccatcgac ggcaacggcg gcagcgatct cgtgttcggc
     2401 ggcggcggca acgacttcat agtcggagac ggcggcgcct tcgccgatga aaggggcaac
     2461 gacacgcttg acggcggtgc gggcgacgac agaatttccg gaatgggagg cgacgattat
     2521 ctggtcggcg gcgacgatga cgattcgctt gccggtggtc acggcaccga cgagctgtac
     2581 ggccaagccg gcgacgacag cctggaggcc gggttcaacg atggcgccga catcgtcgac
     2641 ggcggtgacg gcatcgacct ggtgatcatc actgcgtccg gcgatttcgg cccttccgga
     2701 gacctgatct acaatttctc caaccctggc gcggtctacg cgatcgaggg cggcgcgacg
     2761 atctcgaatg tcgagcgggt cgacatgacc ggcgccagcg gcaacgacca gctgaccggc
     2821 ggcgcctatg ctgaccgctt cctcggcttc tccggcaacg acacgttgcg gggcggcggc
     2881 ggcaacgatt cgctcaatgg aatgaccggt caggactttc tcttcggcga cgcgggtgag
     2941 gacgatttca cctacatcct caccgccgcc ggcgcggccg accgcgacgc gatcgacggc
     3001 ggcgccgaca tcgacttcat cttcctcaac catacccttc cgaccggtcc ggccaattac
     3061 gacttctcaa attcggccgc gacgatcacg cgtcccgatg gcaccacgat cgtcaacgtc
     3121 gagcgcatca aatattgggg cggaaccggc gtcgatacga tcggcggcgg cgcctatgac
     3181 gacgagatac tcggcgcggg cggcaacgat tcgatcctgg gccgggacgg cagcgacagg
     3241 ctcgaaggcg ggtcgggcga ggatttcctc aacggcgatc agggcaacga caaagtctat
     3301 ggcggcgaag gcggcgacac attgttcggc ggcggctaca ctgggccgga gaatgatgag
     3361 ctttacggcg acgcggggac cgacttcctg gtcggcagcg ggggcgacga tatcctcgac
     3421 ggcggcaagg attcagacgt cgacaagctt cacggcggcg tcggcaacga cacttaccac
     3481 atgtacgccg gcgacatctt cgcgaccgcc gccgagagcg gccaggggca ggaagcggag
     3541 attgacggct tcggcggcgg cgtcgaccat gtgatcagcc acgcgtcgag ctacacgctc
     3601 ctgtacagct tcgagaatct cacccttgcc gccaacgcgg tctccggctt cggcagcgag
     3661 gacgacaatg agatactcgg caatgacgac aacaatttga tcggcggcaa tggcggcaac
     3721 gacaagatct tcggcggcac cggccgcgac acgctgagcg gcggcgcggg cttcgacttc
     3781 ttctacctcg ccgacggcga cgcggaggcc ggcgagtcgt tcaacggcgg cgccgactcg
     3841 gaccgcatcg acaccgcgag cgcgcggctc gatctgagcg gcgtcaccat caacgagctc
     3901 gaggcgatcc agacggtgcg gggcgcgggc acggcgttca tcgtcgacac cgccgcccat
     3961 gctgtgctgg tcaatggcgc cggcggctac gacagcctga ccgtcaatga cgagac
//
LOCUS       DASVRK010000109         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_330308, whole genome shotgun sequence.
ACCESSION   DASVRK010000109 DASVRK010000000
VERSION     DASVRK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330308"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VF589_03885"
     CDS             <1..1214
                     /locus_tag="VF589_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEX8256749.1"
                     /translation="ATAAVREASDCDIFLAKVRQIGFEPVVLSGKEEGVMAGQGVLSA
                     IPGADGIVGDLGGGSLELVDLSGGKVAESVSLPLGVLRIAGRSEPSLAKKVAKAIDAT
                     DFARRGRKRPFYLVGGSWRALARLDMLLNDYPLPITQQYRMHSGRPAELQRAIDGLDK
                     TDLRSIPSLSVSRIPTLPHANLLLKNLVEALRPSELIVSSFGIREGLLFSDLPARTRA
                     RDPLLEAVREAGGVLGRFGEHGALLDRWIDPIFEDEADAARLRLAACLLADVAWQAHP
                     DFRAERGVDMALHGNWVGIDAPGRVMVAQALFCNFGGGREFEQSDVAALCTPAQLRHA
                     CHWGLAMRLGQRLSGGAAAALQRSRLLLRGEMLRLELKAGDEAIYGETVQRRLKTLAA
                     AFGRGMEAVAV"
BASE COUNT          216 a          550 c          506 g          234 t
ORIGIN      
        1 tcgccaccgc cgccgtccgc gaggccagcg actgcgacat attcctcgcc aaggtgcggc
       61 agatcggctt cgaaccggtc gtcctgtccg gcaaggagga aggcgtgatg gcggggcagg
      121 gggtgctctc cgccattccc ggcgccgacg gcatcgtcgg cgatctcggc ggcggcagcc
      181 tcgaactggt cgacctcagc ggcggcaagg tcgccgagag cgtctcgctt ccgctcggcg
      241 tcctgcgcat cgccggccgc agcgagccga gcttggccaa gaaggtcgcg aaggcgatcg
      301 acgccaccga cttcgcccgc cgcggccgca agcgcccctt ctacctggtc ggcggctcgt
      361 ggcgcgcgct cgcccggctc gacatgctgc tcaatgatta tccgctgccg atcacccagc
      421 aatatcgcat gcacagcggc cgccccgccg agctccagcg cgccattgac ggcctcgaca
      481 agaccgacct tcgcagcatc ccctcgctgt cggtctcgcg catcccgacg ctgccgcacg
      541 ccaatctgct gctcaagaat ctcgtcgagg cgctgcgccc gagcgagcta atcgtctcca
      601 gcttcggcat ccgcgagggg ctgctgttca gcgacttgcc ggcgcgcacc cgcgcccgcg
      661 atcctttgct cgaggcggtg cgcgaggcgg gcggcgtgct cggccgcttc ggcgagcatg
      721 gcgccctgct cgaccgctgg atcgacccga tcttcgagga cgaggccgac gccgcccgtt
      781 tgcgcctcgc cgcctgcctg ctcgccgacg tcgcctggca ggcccatccc gatttccgcg
      841 ccgagcgcgg cgtcgacatg gcgctgcacg gcaattgggt cggcatcgac gcgcccggcc
      901 gggtgatggt tgcccaggcc ctgttctgca atttcggcgg cggccgcgaa ttcgagcaat
      961 ccgacgtcgc cgccctctgc acgcccgccc agcttcgcca cgcctgtcat tggggtcttg
     1021 ccatgcgcct cggccagcgc ctcagcggcg gcgccgcggc cgccctccag cgcagccggc
     1081 tgctgctccg cggcgaaatg ctccggctcg agctcaaggc cggcgacgag gcgatctacg
     1141 gcgagaccgt ccagcgccgc ctcaagacgc tggccgccgc cttcggccgc ggcatggagg
     1201 cggtggcggt ctagggtaga ggtgtcaggt tagtgtgaac caaaaccgct catcctgagg
     1261 agccgctgag cttgtcgaag cggcgtctcg aaggacgcac catagttctt tgagacgggc
     1321 aggcgagatc gcgttccccg gcgaaggccg gggcccagac tctagcgcag ctggaccccg
     1381 gcttccgccg gggaacaccg cagccgccgt ccgcacgggc tccttcgaga cggggcttcg
     1441 ccggggctca gtccctcctc aggatgagcg gatagatagg atgagcggat agatgagggg
     1501 gaccta
//
LOCUS       DASVRK010000110         9413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_392705, whole genome shotgun sequence.
ACCESSION   DASVRK010000110 DASVRK010000000
VERSION     DASVRK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392705"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..3690)
                     /locus_tag="VF589_03890"
     CDS             complement(259..3690)
                     /locus_tag="VF589_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256750.1"
                     /translation="MIRMFDNRSLRGGAAARKPLVGSGTALGGVLAATLLLAPAAAAA
                     QAGGECGAPEDGVVTCTPEGNPYPRGVYYDTNEDLTLNLADGVVAQQRVRVDGQDASL
                     TINIAEGASVSGSGFLNSAVEANARGAGDVVIDARGSIASEGFLSNGIRTIADEGSVT
                     ITAGDIDSVGGSGVSVSGGTGTVRVETSGQVRAENGAGLFGDSTNTVATVFVLSDGRV
                     EIVNSGLIEQIGDPDDPNGVGLAHGGIDVQADFRPATGIARDVVISGEGSVVASGYGA
                     TAIDARGDTISIRQASATARGEFAVAIGASAAGAIAIDVDEAISERSSAIIAVSRGGD
                     VTIAFDTLSAFGLGAYGESATGAVSIEAGDVDIGPDVNGVGGAGVQIKAATRAEANIT
                     GTIAGRDSFGVAVESAEQTEVTVADTGAIDVDLDGIHVTTDGSATIVNQGSIASARED
                     GIEVEVAGDVIIDGAGSIATAGDGGRAVSATGAAIAIRQGSLATTGAGAAGVEAFGTS
                     DVTVDVGSAASAQWDAIDARSSGGDVAIAFDTLSAQGDGAYGESQNGRVTIAAGDAQV
                     EGAASSAVFAVGATEAAVTVEGEVSARRGYGVRIDSAGGASLDVAAGASITGGIDAVN
                     LTSGNEGATIVNAGRIGSATGYAINIFGGPVRIDNIGRIDGAVLLGDRNDRLVNNGVF
                     NAVRDSNFYGGNDLFDNRGTLLVLAGSHHEPGAVRFLGLEQFTNSGTVDLANGRSGDR
                     LVLGGDYRGQDGRLQLDAGDRLIVRGAATGRTLVDAGGLDGLLQSRNIVQAGEGSAAD
                     AFTADGVNVGFVEYGVRFNAARNNYALAGVAGDAAYRTARYAEGARQLWYQSSDAWSP
                     HMRALRDGPGGEYTDRLWNQSYGSVHERRGVESRQAGGVATEADIGTTQDYFGRQFGY
                     DLIGGAGRPLTAGVTVGYVDSSLKFRGAAARADFIAVFGGGYAAFRSGPLFANLLGKY
                     DRYWIGARDSFADFEEDLTGSAFGVTGEVGLRFGARRLFVEPVASLSWVRTDVEDLAA
                     AGSTIAFGSDDGMRGKLGFRAGSIPDPRARTRLSFYVGANAVKEFGDGADATLLNNDR
                     AVLIATDPIGLYGEGLMGLTIVGGRMSGFFEGYANVGGREDLRGGGARAGMRMSF"
     gene            3755..5065
                     /locus_tag="VF589_03895"
     CDS             3755..5065
                     /locus_tag="VF589_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8256751.1"
                     /translation="MSETRGVPEEVAGGEGEAALRREIGFLGSAFLAFNGVVGAGIFA
                     LPESLLTQFGTFSPWLFPLFGGLVLLIAVPFARVASYFPLSGGPVLYTARFGPLVSFQ
                     VGWLYYVARVTALAANSNLFVTYAASLWPPLSSGVPRGIAILALCGGLTLVNIVGVRR
                     AIRTLDALTLVKAAPLVAMAIWGLAVSADPVPAFTGLPPLSGVEAAALLVLYAFVGFE
                     NSVVPAGETANARRTIPRALIVTVVATIALYLLVQLAYVAVMAPGSGGDAPLVAFGIA
                     LAGPAGGLLLTAAALASLAGNLSGSMTSTPRATFALARKGLLPAWFGRVSERYHTPAN
                     SILFMGAAGALLALTGSFVWLAVVSTLARLFVYGLSLAALPGVQREAGERPGRALYPW
                     LAGGLAVCFWAMLQSQWPAWRMLLALVAVGSLLYWLARRPLRRR"
     gene            complement(5052..6158)
                     /gene="mnmA"
                     /locus_tag="VF589_03900"
     CDS             complement(5052..6158)
                     /gene="mnmA"
                     /locus_tag="VF589_03900"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX8256752.1"
                     /translation="MRADFQLDRPASEARIVVAMSGGVDSSVVAALAARTGAEVIGVT
                     LQLYDHGAAVQRTGSCCAGQDIYDARAVADRLGIAHYVFDYESRFRDSVIDRFADEYM
                     KGRTPIPCIACNQGVKFTDLLSLARELGADCLATGHYVRRVLGPSGPELHRAADPARD
                     QSYFLFATTREQLGFLRFPLGDMPKAAVREVARELALAVADKPDSQDICFVPDGDYAS
                     VVKKARPEAAQPGDIVDGEGRVLGRHPGLIHFTVGQRRGLEIGGQPEPLYVVRLEPEQ
                     RRVVVGPKAALAVRAARLGGLNWLGEDQREGLTAKVRSMAKPAPVSFSGDSLRFERPE
                     YGVAPGQAAVIYAGERVLGGGWIEATVPAELIAA"
     gene            6247..6531
                     /locus_tag="VF589_03905"
     CDS             6247..6531
                     /locus_tag="VF589_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256753.1"
                     /translation="MDLGQFRRQGAKLFEESWAAAPDYGLFVASLAAMQASSAAARAE
                     AGGRRLRAANADDSRPLVDILLAAPSWRASVVAQGGCFDRIIAADRAREA"
     gene            6648..6947
                     /locus_tag="VF589_03910"
     CDS             6648..6947
                     /locus_tag="VF589_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX8256754.1"
                     /translation="MIENQKIRPAQVIGPLGEPLTLATLPPPSTTRWVVRRKAEVVAA
                     VNGGLLSVDEACEHYNLTLEEFASWQRAVDRSGMPGLRVTRIQHYKSLYERQQKF"
     gene            7142..7411
                     /locus_tag="VF589_03915"
     CDS             7142..7411
                     /locus_tag="VF589_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8256755.1"
                     /translation="MNLIIALIIGGIIGWLASIVMRTDAQQGILLNIVVGIVGSLLGG
                     FILGPLLGGGNLLNGALDIRTLLVALLGAIILLAIVNLFRRGRVR"
     gene            7518..7820
                     /locus_tag="VF589_03920"
     CDS             7518..7820
                     /locus_tag="VF589_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8256756.1"
                     /translation="MGFLFAILLGGIIGWLASIVLPRPDHKPLWPDILAGMFGGLLGG
                     LMLGPLLGGGNLLESELDMMTLVDAALGAAFVLAIVQFVRRRRQRRRQEVRPGEKP"
     gene            complement(7817..8530)
                     /locus_tag="VF589_03925"
     CDS             complement(7817..8530)
                     /locus_tag="VF589_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405733.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8256757.1"
                     /translation="MIRIVALASLIALSAPAALAAQGVPMQAISGTRLDVVANGEVRR
                     VPDIAQINAGVVTQARTATAAIAQNAQQMARVIAALRRAGIAERDIQTSSINLNPEYR
                     HEENRPPILTGYRAGNEVTVRFRDIGETGAILDALVAQGANQINGPSLSIDKPEAALD
                     EARQAALATARARAEVYARALGRRVTRILSISESGAASPPYPMPVMGRAMAQDMAESK
                     IVPGEQTLTVSLSVSFEIG"
     gene            8843..>9413
                     /locus_tag="VF589_03930"
     CDS             8843..>9413
                     /locus_tag="VF589_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8256758.1"
                     /translation="MNFETASWRRSAIEGDPVEVDVDALAQRRKRRRIIIAVAVALIL
                     AAAAVFFMTREKPASDPAAGGAAEKALPAVTVVVPGRQQVSRAISATGTLAARRDMPV
                     GIAGEGGMVARVLVEPGQWVGAGQTLAVIERSVQAQEAQQLAASIEVARADARLAQQE
                     LDRAQALVSRGFVSKADVERRIATRDAANA"
BASE COUNT         1270 a         3283 c         3328 g         1532 t
ORIGIN      
        1 ctgttgctgt catgcgcctg gccggccttg ccgccatggc cgccgccggc tttcttgttg
       61 tcgtcggtcg agcccgaccc gcggccggta aggttgttgc cgcgcgtatc gtggccgctg
      121 tcccggcgtt tgccgctcat catcgcctcc gtcaggcccg gcggcagcgc cgggcgtcgg
      181 cacggtcaac gggcggcctg gcgatgggtt gcgcggggac gcctcgggcc gccgcgccgg
      241 tgcggcgggc ggcccggctc agaagctcat gcgcataccc gcccgcgcgc cgccgccgcg
      301 aaggtcctcg cggccgccga cattggcata gccttcgaag aagccgctca tgcggccgcc
      361 gacgatggtg agccccatca gcccctcgcc atagaggccg atcggatcgg tcgcgatcag
      421 caccgcgcga tcgttgttga gcagggtggc gtcggcgccg tcgccgaact ccttcaccgc
      481 attggcgccg acatagaatg agaggcgggt gcgggcgcgc ggatcgggga tcgagccggc
      541 gcggaagccg agcttgccgc gcatgccatc gtccgacccg aaggcgatgg tgcttccggc
      601 cgccgccaga tcctcgacgt cggtgcggac ccaggacagg ctcgcgaccg gctcgacgaa
      661 cagccgccgg gcgccgaagc gcaggccgac ctcgccggtc acgccgaacg cgctgccggt
      721 gagatcctcc tcgaagtccg cgaagctgtc gcgcgcgccg atccagtagc ggtcatattt
      781 gccgagcagg ttggcgaaca gcggaccgct gcggaaggcg gcgtagccgc caccgaacac
      841 ggcgatgaaa tcggcccggg ccgccgcgcc gcggaatttc agcgagctgt cgacatagcc
      901 gaccgtgact ccggcagtga gcggacgtcc ggcgccgccg atcaaatcgt agccgaactg
      961 gcggccgaaa taatcctggg tggtgccgat atcggcttcg gtggcgacgc cgccggcctg
     1021 gcgcgattcg acgccgcggc gctcgtggac cgatccgtag ctctggttcc agagccggtc
     1081 ggtatattcg ccgccggggc cgtcgcgaag cgcgcgcatg tgcggcgacc aggcgtcgga
     1141 ggattgatac cagagctgcc gcgcgccctc ggcatagcgc gccgtgcgat aggccgcgtc
     1201 gccggcgacg ccggccaaag catagttgtt gcgggcggcg ttgaaccgga cgccatattc
     1261 gacgaagccg acattgaccc cgtcggcggt gaaggcgtcg gcggccgatc cttcgccggc
     1321 ctggacgata ttgcgggact ggagcaggcc gtcgagcccg ccggcgtcga ccaaagtacg
     1381 gccggtggcg gcgccgcgga cgatcagccg gtcgcccgcg tcgagctgga gccgcccgtc
     1441 ctgcccgcga tagtcgccgc cgaggaccag ccggtcgccc gagcggccgt tggcgaggtc
     1501 gacggtgccg ctgttggtga actgctcgag cccgaggaac cggaccgcgc cgggctcgtg
     1561 gtgcgagccg gccaggacga gcaaagtgcc gcgattgtcg aacaggtcgt tgccgccgta
     1621 gaaattgctg tcgcggacgg cgttgaaaac gccgttgttg accagccggt cgttgcggtc
     1681 gccgagcagg acggcgccgt cgatcctgcc gatattgtcg atccgcacag ggccgccgaa
     1741 gatgttgatc gcatagccgg tcgcgctgcc gatccggccg gcattgacga tggtggcgcc
     1801 ctcgttgccg gaggtgaggt tcaccgcatc gatgccgcct gtgatcgacg cgcctgcggc
     1861 cacgtcgagc gaggccccgc cggcgctgtc gatgcgaacg ccgtagccgc gtcgcgcgct
     1921 cacctcgccc tcgaccgtca ccgccgcctc ggtcgccccg accgcgaaca ccgccgacga
     1981 tgccgcgcct tcgacttggg cgtccccggc cgcgatcgtc acccgcccgt tctggctttc
     2041 gccataggcg ccgtcgccct gcgccgagag ggtgtcgaag gctatggcga cgtcgccgcc
     2101 cgaggagcgc gcgtcgatcg cgtcccattg ggcgctggcc gcgctgccca catcgaccgt
     2161 cacgtcggac gtgccgaacg cttccacccc ggcggcgccg gcgccggtcg tggcgaggct
     2221 gccttggcgg atggcgatcg cggctccggt ggccgagacg gcgcgaccgc cgtcgcccgc
     2281 ggtcgcgatc gagccggcgc cgtcgatgat gacatcgccg gccacctcga cctcgatgcc
     2341 gtcctcgcgc gcgctggcga tcgagccttg gttgacgatg gtcgcgctgc cgtcggtggt
     2401 gacgtggatg ccgtccaggt cgacgtcgat cgcaccggtg tcggcgacgg tcacctcggt
     2461 ctgctccgcg ctctccacgg cgaccccgaa gctgtcccgt cccgcgatgg tgccggtgat
     2521 gttggcttcg gcgcgagtcg cggccttgat ctgaacgccc gcgccgccga ccccgttgac
     2581 gtcggggccg atgtcgacgt cgccggcttc gatgctgacc gcgccggtcg cgctctcgcc
     2641 atacgcgccg aggccgaacg cgctcagcgt gtcgaaggct atcgtgacgt cgccgccgcg
     2701 cgagacggcg atgatggccg aggatcgctc gctgatcgcc tcgtcgacgt cgatcgcgat
     2761 cgcgccagcg gcgctggcgc cgatcgcgac ggcgaactcg ccgcgcgcgg tcgcgctcgc
     2821 ctggcggatg ctgatcgtgt cgccgcgcgc gtcgatcgcg gtcgcgccgt agccggacgc
     2881 caccaccgag ccttcgccgc ttatgacgac gtcgcgggcg atcccggtcg ccggccggaa
     2941 atcggcctgg acgtcgatcc cgccatgggc gagcccgacg ccgttcgggt cgtcgggatc
     3001 gccgatttgc tcgatcaggc cgctattgac gatctccacc cgcccgtcgc tgagaacgaa
     3061 cacggtggcg acggtgttgg tgctgtcccc gaagaggcct gcgccatttt ccgcgcgaac
     3121 ctggccgctc gtttcgaccc gcacggttcc ggtgccgccg ctgaccgaga cgcccgagcc
     3181 gccgacgctg tcaatatcgc cggcggtgat ggtgacggag ccctcgtccg cgatcgtccg
     3241 gatgccgttc gacaggaacc cttcgctggc gatcgatccg cgcgcgtcga tgacgacgtc
     3301 gccggcaccg cgcgcattgg cttcgacggc gctgttcaga aagccgctgc cgctgaccga
     3361 cgcgccttcg gcgatgttga tggtgagcga agcatcctgc ccgtcgacgc gcacccgctg
     3421 ctgcgcaacg acgccgtcgg cgaggttgag cgtcaaatcc tcattcgtgt cgtaatagac
     3481 gcccctcgga tagggatttc cctccggcgt gcaggtgacg acgccatcct ccggagcgcc
     3541 gcattcgccg ccggcctggg ccgcggccgc ggcgggggcg agcagcaatg tcgccgcgag
     3601 cacgccgccg agcgcggtgc ccgagccgac aagaggcttg cgcgcggccg ccccgccacg
     3661 caggctgcgg ttatcgaaca ttcggatcat ggcgcgcccc catgtttatg tgacacctga
     3721 gtagggcgat tgctggaaag gcgctattca aaaaatgagc gaaacgcgcg gagtgccgga
     3781 ggaggttgcg ggcggcgagg gcgaagccgc tttgcgccgc gagatcggct tcctcggctc
     3841 ggccttcctg gccttcaacg gcgtggtcgg cgccggcatc ttcgcgctcc ccgaaagcct
     3901 gctgacgcag ttcggaacgt tcagtccgtg gctgttcccc ttgttcggcg gcctggtgct
     3961 gctgatcgcg gtgccgttcg cccgcgtcgc ttcctatttt ccgctgtcgg gcggacccgt
     4021 cctctacacc gcgcggttcg ggccgttggt gtcgttccag gtcggctggc tctattatgt
     4081 ggcgcgggtg acggcgctcg ccgccaacag caatttgttc gtcacttatg ccgcctcgct
     4141 gtggccgccg ctctcctccg gcgtgccgcg cggcatcgcc atcctggcgc tgtgcggcgg
     4201 cctcaccctc gtcaacatcg tcggcgtgcg ccgggcgatc cgcacgctgg acgcgctcac
     4261 tctggtcaag gcggcgccgc tggtggcgat ggcgatctgg ggcctcgccg tctccgcgga
     4321 cccggtgccc gccttcaccg gcctgccgcc gctcagcggg gtggaggcgg ccgccctgct
     4381 cgtgctctac gccttcgtgg ggttcgaaaa ttcggttgtg ccggccgggg agaccgccaa
     4441 tgcgcggcgc accatcccgc gcgcgctgat cgtcacggtg gtggcgacga tcgcgctcta
     4501 cctgctggtt cagctcgctt acgtggcggt gatggcgccg gggagcggcg gcgatgcgcc
     4561 gctggtcgcg ttcggcatcg cgctggccgg gccggccggc gggctgctgc tgaccgcggc
     4621 cgcgcttgcc tcgctcgccg gcaatctcag cggctcgatg acatcgacgc cgcgcgccac
     4681 cttcgcgctc gcccgcaagg gcctgctgcc ggcctggttc ggccgggtga gcgagcgcta
     4741 ccataccccg gccaattcga tcctgttcat gggggcggcc ggcgccctgc tcgcgctcac
     4801 cggcagcttc gtgtggctgg cggtggtgag cacgctcgcc cgcctgttcg tctacggcct
     4861 cagcctcgcc gcgcttcccg gcgtgcagcg cgaggccggg gagcggccgg gccgtgctct
     4921 ctacccgtgg ctggcggggg ggctcgcggt ctgcttctgg gcgatgttgc agtcgcaatg
     4981 gccggcctgg cggatgctgc ttgccctggt cgcggtcggc agcttgcttt actggctggc
     5041 acgccgcccg ctcaggcggc gatgagctcg gccggcaccg tcgcctcgat ccagccgccg
     5101 ccgagcaccc gctcgcccgc atagatcacc gccgcctggc cgggcgcgac tccatattcg
     5161 ggccgctcga agcggaggct gtcgccggag aagctgaccg gagccggctt cgccatcgac
     5221 cggaccttgg cggtgagccc ctcccgctgg tcctcgccga gccagttgag gccgccgagg
     5281 cgcgcggcgc ggaccgccag cgccgccttg gggccgacca cgacccggcg ctgctcgggc
     5341 tccagccgca cgacgtagag cggctcgggc tgaccgccta tttcgaggcc gcggcgctgg
     5401 ccgacggtga aatggatgag ccccggatgg cggccgagca cgcggccctc gccgtcgacg
     5461 atgtcgcccg gctgcgccgc ctccggccgc gcctttttca ccaccgaggc atagtcgccg
     5521 tcgggtacga agcagatatc ctggctgtcg ggcttgtcgg cgacagcgag cgccagctcg
     5581 cgcgccactt cgcggaccgc cgccttgggc atgtcgccga gcgggaagcg caggaagccg
     5641 agctgctcgc gggtggtggc gaagaggaaa tagctctggt cgcgggccgg atcggcggcg
     5701 cggtgcagct cgggcccgct tggccccagg acccgccgca cataatggcc ggtggcgagg
     5761 caatcggcgc cgagctcgcg cgccaagctc aacaggtcgg tgaacttcac cccctggttg
     5821 caggcgatgc acgggatcgg tgtgcgcccc ttcatatatt cgtcggcgaa gcggtcgatc
     5881 acgctgtcgc ggaagcggct ttcataatcg aacacataat gggcgatgcc gagccggtcg
     5941 gcgaccgcgc gggcatcgta aatgtcctgg cccgcgcagc agctgccggt ccgctgcacc
     6001 gccgcgccat ggtcgtagag ctggagcgtc acgccgatca cttcggcgcc ggtgcgcgcg
     6061 gcgagcgcgg ccacgaccga gctgtcgacg ccgcccgaca tggcgacgac gatccgggcc
     6121 tcgctggccg gccggtcgag ctggaaatcc gcgcgcatgc ggccgccata tagggtgaag
     6181 gcggcgccgc gccagtctgc cccggccttt accggggatt cagccttgtc cgctaaggcc
     6241 tgggccatgg atctcggtca attccggcgc cagggagcga agcttttcga ggagagctgg
     6301 gcagccgcgc ccgattacgg cctgttcgtg gcgagtctcg cggcgatgca ggcgtcgagc
     6361 gcggcggcgc gcgcggaggc gggcgggcgg cggttacgcg cggccaatgc cgacgacagc
     6421 cggccgctgg tcgacatttt gctcgccgct ccgtcctggc gcgcctcggt ggtggcgcag
     6481 ggcggctgct tcgaccgcat catcgccgcc gaccgggcgc gggaggcgta aaccgagatg
     6541 accgggaaat ttaccttcgc attttagcct tccttcaatg cctcgcggct aaggctggga
     6601 ggtgagaaca gacgatcccc ggccaaacgg gattttttga ggtacgaatg atcgagaacc
     6661 agaagatacg tccggctcag gtgatcggcc cgctcggcga gccgctgacc ctggcgacgc
     6721 tgccgccgcc gtcgaccacc cgctgggtgg tgcgccgcaa ggcggaagtg gtcgccgccg
     6781 tcaatggcgg tcttctgagc gtcgacgaag cctgcgagca ttacaatctc acgctcgagg
     6841 aattcgcctc ctggcagcgc gccgtcgatc gttccggcat gcctggtctt cgcgtcaccc
     6901 gcatccagca ttataaatcg ctgtacgagc gccagcagaa attctgaagc ggggctcccc
     6961 gggggagccg ccgcgccttc aatactttcc cgaaacgatg taaggcttgc gccgcctgca
     7021 gccgagttgc gccgccagac gtgcgacgga cggcaggaac aagcagcgcc ttcgcgcgtc
     7081 ttggaatcgg aacgccgctg aaaccgtttg gttcttcggc tgcgaagaca ggagggtaag
     7141 tatgaatctc atcatcgctc tgatcatcgg gggcatcatc ggctggctcg cgagcatcgt
     7201 catgcgcacc gacgcccagc agggaatcct gctcaacatc gtggtcggca tcgtcggctc
     7261 gctgctcggc ggcttcattc tcgggccgtt gctcggcggc ggcaacctgc tcaacggcgc
     7321 gctcgatatc cggactttgc tggtcgcgct gctcggcgcc atcatcctgc tcgcgatcgt
     7381 gaacctcttc cgccgcggcc gcgtgcgcta agagccacga ctatcgcttg aagctggggc
     7441 cgccgcgcgc atgcgaggcg gcccctttct ttttcacctg gcgccgtccg gccgtctttc
     7501 gcccggcagg agcatgtatg gggttcttgt tcgccatcct cctcggcggc atcatcggct
     7561 ggctggccag catcgtgctg ccgcgcccgg accataagcc gctctggccg gacatcctcg
     7621 ccggcatgtt cggcgggctg ctcggcggat tgatgctggg cccgctgctc ggtggcggca
     7681 atctgctcga aagcgagctc gacatgatga ccctggtcga cgccgcgctc ggcgccgcct
     7741 tcgtcctcgc catcgtccag ttcgtccgtc gccgccggca gcggcggcgt caggaggttc
     7801 ggccgggcga gaagccctag ccgatctcga agctgaccga gagcgacaca gtgagggtct
     7861 gctcgccggg cacgatcttg ctctccgcca tatcctgcgc catcgcgcgg cccatcaccg
     7921 gcatcggata aggcggcgat gcggcgccgc tctcgctgat cgacaggatc cgagtcactc
     7981 gccgcccgag cgcccgcgcg tacacttcgg cgcgggcgcg ggcggtcgcc agcgccgcct
     8041 gccgcgcctc gtccagcgcc gcctccggct tgtcgatgct gagcgagggt ccgttgatct
     8101 ggttcgcccc ctgcgcgacg agggcgtcga ggatcgcgcc ggtctcgcca atgtcgcgga
     8161 accggaccgt cacttcgttg cccgcccgat aaccggtcag gatcggcggc cggttctctt
     8221 cgtggcgata ttcggggttg aggttgatcg aactggtctg gatgtcgcgt tcggcgatgc
     8281 cggcgcggcg gagggcggcg atcacccgcg ccatttgctg cgcgttctgg gcgatcgcgg
     8341 cggtcgcggt ccgcgcctgg gtaacgacgc cggcgttgat ctgggcgatg tcgggcaccc
     8401 gccgtacctc gccgttggcg accacgtcga gccgggtgcc ggaaatggcc tgcatgggca
     8461 cgccttgggc ggccagcgcc gccggcgcgg agagggcgat cagcgacgcc agtgcgacaa
     8521 tacggatcat gttcgagctc ccgatgattt cgtgcccgga atatcgagac cggcccctgc
     8581 acgccacctg aacgttcggc gcgcggcgga tgcccttctt gagagcgccc ggcgggccca
     8641 ccatctaagg atcgggccgc tcgtcgaaaa atcaggcacc cctgccgaac ggcaatggga
     8701 ataaggccgg ttccggtgta tggagcgccg gggcgcgggg cgggctcgcg actgacatat
     8761 ggggcttgag ccgggtgact tattcggcta acacagtcgc gacgcggcgc ggccgcctgg
     8821 cggtgtggga aagatgaaga cgatgaactt cgaaactgcg agctggcggc gttccgccat
     8881 cgaaggggac ccggtcgagg tcgacgtcga cgcgctcgcg cagcgccgca agcgccgccg
     8941 gatcatcatc gcggtggcgg tggcgctgat cctcgcggcc gcggcggtct tcttcatgac
     9001 gcgcgagaag ccggcgtcgg atccggccgc gggcggagcc gccgaaaagg cgctgccggc
     9061 ggtgaccgtg gtcgtcccgg gccggcagca ggtctcgcgc gcgatttcgg cgacgggaac
     9121 gctcgccgcc cgccgcgaca tgccggtcgg catcgccggc gaaggcggca tggtcgcgcg
     9181 ggtcctggtc gagccgggcc agtgggtcgg cgccggccag acgctggcgg tgatcgagcg
     9241 ctcggtccag gcccaggagg cccagcagct cgcggccagc atagaggtcg cccgcgccga
     9301 cgcgcggctc gcgcagcagg agctcgaccg cgcccaggcc ctggtctcgc ggggcttcgt
     9361 ctcgaaggcc gacgtcgagc gccggatcgc gacgcgcgac gccgccaacg ccc
//
LOCUS       DASVRK010000111        13766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_330879, whole genome shotgun sequence.
ACCESSION   DASVRK010000111 DASVRK010000000
VERSION     DASVRK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330879"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VF589_03935"
     CDS             <1..615
                     /locus_tag="VF589_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256759.1"
                     /translation="LVRGGEASAPPRQHSYEVPEELCCAGAGKPDFMIWNEIVREPRG
                     GALVGLVFARKTIGEHGFRSYQRRLHLLRVPREDGGAPVPVLDAPLSGMVDQPVCRSV
                     EDQRARPDACMDRFYFSTLFTLVPEIAPVEPANLIMIATARTSPGRRTRTKGPVPRGA
                     IERTTDDDARDPLCSYTRSYYFDEASGRYLPDAPLPDCSDHLEP"
     gene            complement(662..3379)
                     /locus_tag="VF589_03940"
     CDS             complement(662..3379)
                     /locus_tag="VF589_03940"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEX8256760.1"
                     /translation="MGDQRNEAAAGDAAKTGPNGAAPAGHAGGAGAAKSIILFSDGTG
                     NSSAKLFKTNVWRMYEAVDLGPATPGKRKQIAFYDNGVGTSGFKPLAILGGVFGIGLR
                     QNVLDIYRYACRNYNPGSPLQATEQDGAEGDHIYGFGFSRGAFTMRTAIAMIAKVGLL
                     ETTREADLHQRSKEAWRAYRAGCAPRSGWSLWNLYSKLFRRNRARKDQDLLERLRAEN
                     YHPVIRFVGVWDTVAAYGGPIAEITRGIDNWIFPLGMPNYELSPHVRCARHALAIDDE
                     RDAFHPLLWDELAEEGLRQEHPAEKMPWLHPKPHDSPRLQQVWFTGMHADVGGGYPDE
                     SLSYVSFLWMKAEAEKAGLRTVKVITDRYRALANSGGPIHDSRSGAGVYYRYQPRNIG
                     AWIHPADAESIVTDPELKDKAGKSKGLLQTAHVHESVLARIAASGNRYAPFGIPTNIK
                     IVPPQPGGETAQQADTETPVAPPAEEQEARPMLSPELTEHLEDPIVGEARSKAMAAVW
                     DHVWIRRITYFTTLFLTLALLLMPWWVELAPDPPFLADGRNWIGGVIRLLAIVTPAFL
                     DPLIDTMADNAFYFLVIATAIGMLMWRTSVRERRLRDLCRAIWAESVDVGGPPPIERS
                     PLWVRGLRTSKLYRRPMRILKWRVLPWLAALVITAFLLWLLVGLVVQIWLPFAERGAG
                     LCERRPPHAEKEMARADFTPAATCAPVDLAVERDQRYIVEIDVVDSWTDGGKPADPAG
                     LDAGEVGFSGYVGVPFRRVITADYLQPVAEIRTRDGGVHIQALEMRQQGDSTASWRGD
                     FVAARNGALALFANEAVLPGMKSYFYDGGNYGNGGSACVTIRRAERAGDPAPLGRPGT
                     VCATLDERTEADQLRRQQQLEARRHRPRSRHRPESAVPAAPAPAPRAPR"
     gene            3555..5282
                     /locus_tag="VF589_03945"
     CDS             3555..5282
                     /locus_tag="VF589_03945"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX8256761.1"
                     /translation="MRPVRAARPLPASVALLLALLAAGCASKPELPAAGPIARPAPAA
                     YDLVIRGGTIYDGSGGAPYKGDVAISGDRIVAAGPALKGAAKREIDARGLAVAPGFIN
                     MLSWANETLLQDGRGESDIRQGVTLEVMGEGRSMGPLNPQMKAELQNRQIEIKYPVTW
                     TSLGQYLEELEARGVSPNVASFVGAATVRTHELGEADVDPSPEQLGRMRALVRQAMDE
                     GAMGVGSSLIYVPATFAETDELVALAAEAGRCGGMYISHIRNEGPRLLASIDELIEIG
                     RRSGARAEVYHLKAAGPANWHLMDAAIARIAAARAGGLPITADMYLYTASGTGLDSTL
                     PIWVREGGHDAMVARLKQPEMRARVVAELRGARPGRDWSAVQPSSFHNPALRHYANKR
                     LSEIAALRGQSPEETAIDLVVEDDSRVGTIFHSMSEDKVRLGLVQPWVSFGSDAGAIP
                     AEPPFTDTPVHPRTYGNFARLLGRYVREEKLVTLAEAVRRLTSLPATNLGLSDRGRLK
                     PGYFADLAIFDPATVGDRATYERPHQYAVGMRHVLVNGVPVLKDGAHTGARPGRVVRG
                     PGWRKCRAG"
     gene            5434..5805
                     /locus_tag="VF589_03950"
     CDS             5434..5805
                     /locus_tag="VF589_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX8256762.1"
                     /translation="MIGSLPRREREIFELLCSAGEATAADVRKAMADPPSHSAVRTML
                     ARLEAKGLVTHRAVDQAYVYKSVPQPAKVRESALKQMVKTFFDGSAASAATALLGLTR
                     NLRPEELDALQRAIDEAKERP"
     gene            5802..7385
                     /locus_tag="VF589_03955"
     CDS             5802..7385
                     /locus_tag="VF589_03955"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX8256763.1"
                     /translation="MTSFAFFVEMAWKSALIAGAALLLAGLVRSWSAADRTALLRLAI
                     VMLLGLPAVALLLPGIEVEMAVPAAAEAEPLLRSALPAPAAMPADAAASVAVAPAAGR
                     DDPGMLVALLYLGGLMMVGLRLAGGLWTLRRWTAAASPLDCPEWQAALARALADPAAP
                     RFRLLVSYEAPSPLSWGWRRPVILLDADTLRRADDADAILAHEIAHIARGDWPTLILS
                     RLAVALFWFNPLVWWLERVTVQEAEEAADGAAAGRVEPARYAQILLNCAQQAGTLPVS
                     SIASGCLRRRIRAVLDGSFSGRRRGSVWTAAAMLGCAAVAAPVAATRLVPALAAAPAA
                     PARPVARIGPLTISMTEAPVRIAAAPLPPEAPMPLPAAEAPLPPEAPAVASAAPEAAL
                     PSPPAPVAAPAPPAPPAPPAPAAPPRPPAPVLDVETLVQMRVHGVTGDYIEALAAIGP
                     HYRRLTPEQMVRMRIHGVTPDYVRDLAASGYPAIGAEQLIRMKIHGVTPAGAKRAALA
                     PGARPSAEELIDRAIHRKL"
     gene            7397..8392
                     /locus_tag="VF589_03960"
     CDS             7397..8392
                     /locus_tag="VF589_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256764.1"
                     /translation="MFVRTVLALAAAALVQTASASAAEASFPIGWTMSPAEGRPAMVQ
                     LELSYRSGGSVGNTSRPVAVADLQGLGEAQLAGPGGPAAFRIVRDAGTIACQGVVRQR
                     RGTGECRFAGDPRFAAELARRGIGRPSGYEQFQLALHGGDLAVVAELERQKYARPTVE
                     QLVGLGIHGAGLSYLRALDAAGYRVGSADKLVEMRIHGVSADYIAELRTLGFARPSAD
                     QLVRMRIHGVSADYARELAALGYKRLSAEELVNMRIHGVKPELVRELARLGYRGVTGE
                     QLTRMAIHGVSPGFVREMAAAGYRQVPVEDLIRLRIHGVTPEEARRANAAFRRGD"
     gene            8446..9660
                     /locus_tag="VF589_03965"
     CDS             8446..9660
                     /locus_tag="VF589_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX8256765.1"
                     /translation="MKKLVIAAAMAAAWVAPLTGAQPASEPPPAARTAAEGNAEFGVA
                     LYRKVAERPGNVFLSPISIVGAFGPVSAGAEGETLAEIARALRLPGGAAGHEELGAML
                     RGLEREREGVTLSIANALWVQQGFGLKPAFEKTARKQYDARVEALDFARNEQAAARIN
                     GWVKEETRGRIGELINARSLNRDTALVVTNAVYFLGDWATAFRTAATANEPFHLPGGE
                     TRTVPLMSRQDPFRYFETDEFQAIDLPYKDERLSMSLFLPKARDGLPPFEAGLDAARL
                     REWLGRLDAERPRAVRLHLPKLRIGETYNLIPPLGEMGIAAAFTPGRADLSGIADREL
                     FISQVIHKTFLKVDEKGTEAAAATGIGIQVTSAPPPPPIFRADHPFFLVIRDKASGAI
                     LFLGRIVSPDQA"
     gene            9773..10531
                     /locus_tag="VF589_03970"
     CDS             9773..10531
                     /locus_tag="VF589_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8256766.1"
                     /translation="MDRLKTIALALLLAAPGASLQAAPAEVAAAVAAKERPAEATKLD
                     ESRKPAEVLGFLGLERGDRALDLFTGSGYYAEIMAKAVGPQGSVVAWEPANFYNDEVK
                     KVWAELPGRAPNARLLVEAADAMALPAASFDFVLMHMIYHDFYWESAEYKYPRLDPDA
                     VLKTVFAATKPGGIVGVVDHVAEPGGDTRAVVDKLHRIDPAVIRADFERAGFVFDGES
                     ELLRNPGDDRSKLVFDPAVRGKTDRIVYRFHKPA"
     gene            complement(10541..11368)
                     /locus_tag="VF589_03975"
     CDS             complement(10541..11368)
                     /locus_tag="VF589_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX8256767.1"
                     /translation="MNELPLQLNAEGAAAAAVPPSPAPPSLRRRLYRQLDPRAWTKPG
                     LSPLNTLVTLAIIVATAAAIVETEPLVTAGRERLFDAAELGFGLFFLAEYAARLWTVV
                     EEEGARSHWRRRLAFVRSPAAIFDLLAILSALLPFIGASGAMLRLVRLLRIFRLAKLG
                     RMSAALSNLASAVLSRRFELAATVGLALMLVVFGATALYWIEGEIQPDKFGSIPRALW
                     WAVVTLTTIGYGDVFPVTPLGKLVAAFVAIAGIGLIAMPTGILAAAFSEACQRDKAV"
     gene            11525..12445
                     /locus_tag="VF589_03980"
     CDS             11525..12445
                     /locus_tag="VF589_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256768.1"
                     /translation="MADDRYSQERGGGRRDSMFNDDERDSWERHRRSEMGWGRSSEGG
                     GHEGGGFFERAAGEVRSWFGGDDDGQRGDGQRGGGGSGGEPWGGGGWDGNEGVGGRSR
                     PSHYGSEHGYGGFQGDYGAGGGQGGGGGQGGFGGRGDYAHGRQSFSGGDREGAYGSRE
                     GSYPGAQGQYRGSGSSGWSNNDLSPHPDDHYRSWRERQIAELDRDYEEYCRERQQSFE
                     REFGSWRQGRQGGGAMSSGLSGNMSGVALGGTAATGPMGSGGLTGASPGAGDFAAADE
                     SGAGAGGGGSPAGTPEAAESGGSARGGGRS"
     gene            12502..13173
                     /locus_tag="VF589_03985"
     CDS             12502..13173
                     /locus_tag="VF589_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8256769.1"
                     /translation="MAGYDRYDDDTHARSRDYDRERGGGRGFFGFGGGREPERGGGWS
                     GGRDPERGGLGGGREPERGGGFFGSGRDEHRERGGGFFGGGGHERGHERGGERDYGRD
                     DHRAGLPIEETSRLIGSNKVEGTAVYGRDGHRLGSIYNFMVDKISGRVEYAVMTYGGF
                     LGMGTRYYPLPWRALSYDTRAGGYRIELTERDLQSAPSFDRDSEPRFDRAYGERVHDY
                     YGLNY"
     gene            13292..>13766
                     /locus_tag="VF589_03990"
     CDS             13292..>13766
                     /locus_tag="VF589_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256770.1"
                     /translation="MSKNIPIASAAALALASAAPAQPGGSAETAAKMHEFRAANVPLV
                     AQPLDLSQKSDPIWVKGERPPGNVIARLALKPVEAIVVAEDVGQGSGQALLAALALAG
                     AAGAYSRTADLQGAREQRIYCGATPAGTFKILCLQDQDGDGRLDHAMPGAAEAGVA"
BASE COUNT         2031 a         4598 c         5142 g         1995 t
ORIGIN      
        1 ctggtgcgcg gcggcgaggc ttccgcgccg ccccggcagc acagctatga ggtgccggag
       61 gagctatgct gcgcaggggc cgggaagccg gatttcatga tctggaacga gatcgtgcgc
      121 gagccgcggg gcggggcgct ggtcgggctc gtgttcgcac ggaaaacgat cggcgagcat
      181 ggtttccgat cctatcaacg gcggctgcac ctgctgcggg tgccgaggga ggatggcggc
      241 gctcccgtcc ccgtgctcga cgcgccgctc agcggcatgg tggatcagcc ggtgtgccgc
      301 tccgtcgaag accagcgcgc ccggcccgat gcctgcatgg accgattcta tttcagcaca
      361 ttgttcacgc tggtgccgga gatcgcgccc gtcgagccgg ccaatctgat catgatcgcc
      421 accgcccgga ccagtcccgg ccgccgcacg cgcaccaagg ggccggtgcc gcggggggca
      481 atcgagcgca ccaccgacga cgacgcgcgc gacccgctgt gcagctacac tcgcagctac
      541 tatttcgacg aagcttcggg ccggtacctg cctgacgcgc cgctgccgga ctgcagcgac
      601 catctggagc cgtgacgcgt ccgggcgggg cgcagcgaag ccgcgcccgc tccgcgggcg
      661 ttcaccgagg cgcccgtgga gctggagccg gtgccgcggg gactgcgctc tccggcctat
      721 ggcggctccg gggcctgtgg cggcgggcct ccagctgctg ctgacggcgc aactggtccg
      781 cttcggtacg ctcgtccagc gttgcgcaca cggtccccgg cctgccgagc ggagccggat
      841 ccccggcgcg ctcggcgcgt cggatggtga cgcaggcgct cccaccattc ccgtaattcc
      901 cgccgtcgta aaagtagctt ttcattcccg gcagcaccgc ttcgttggcg aacagcgcca
      961 gcgcaccgtt gcgcgccgcg acgaagtcgc cacgccagct tgcggtactg tcgccctgct
     1021 gccgcatctc cagcgcctgg atgtggacgc cgccgtcgcg cgtgcggatc tcagccaccg
     1081 gctgcaggta gtcggctgtg atgacgcgcc ggaacggaac gcccacatag ccgctgaagc
     1141 cgacctcgcc agcgtccaaa cccgcgggat cggcgggctt cccgccatcg gtccacgaat
     1201 cgaccacgtc gatctccacg atgtagcgct ggtcccgctc gaccgcgagg tcgacgggcg
     1261 cgcaggtcgc cgccggcgtg aagtcggctc gggccatctc cttctcggca tgcggagggc
     1321 gacgttcgca cagccccgcg ccacgctccg caaagggaag ccagatctgc acgaccagcc
     1381 cgacgaggag ccagagcagg aaggcggtga tcacgagcgc cgccagccac ggcaggaccc
     1441 gccacttgag gatgcgcatc ggccgccggt agagtttgct cgttcgcagc ccgcgaaccc
     1501 atagcgggct ccgctcgatt ggcggtggac cgccgacgtc gaccgattcc gcccagattg
     1561 cgcggcacag gtcccgcagc ctgcgctcgc gcacgctggt acgccacatc agcatcccga
     1621 tcgccgtcgc aatcaccaga aagtaaaagg cgttgtccgc catcgtgtcg atcagcgggt
     1681 cgaggaaggc cggcgtgacg atcgcgagca gccggatgac gccgccgatc cagttacggc
     1741 catcggcgag gaacggtgga tcgggcgcca actcgaccca ccatggcatc agaaggagcg
     1801 cgagggtcaa gaagagcgtg gtgaaatagg taatgcggcg gatccagacg tgatcccaga
     1861 ccgccgccat cgccttcgat ctcgcctcgc cgacaatggg gtcttcgaga tgctcggtga
     1921 gctcgggcga cagcatcggc cgcgcctcct gctcctcggc cggcggcgcc accggcgtct
     1981 cggtatccgc ctgctgggcc gtctccccgc cgggctgcgg cggaacgatc ttgatgttcg
     2041 tcggaatccc gaacggcgca tagcggttcc cgctggcggc aatgcgcgcc aacacgctct
     2101 cgtggacatg ggcggtttgg agcagcccct tggacttacc cgccttgtcc ttgagctccg
     2161 gatcggtgac gatcgattcg gcatctgccg ggtggatcca agcgccgata ttgcgcggct
     2221 ggtagcggta gtagaccccg gcgcccgacc ggctgtcgtg gatcggtccg ccgctattcg
     2281 cgagcgcccg atagcggtcg gtgatcacct tgaccgtccg caggcccgcc ttttccgcct
     2341 ctgccttcat ccacaggaac gagacgtaac tgaggctttc gtcgggatag ccgccgccga
     2401 catcggcgtg catgccggtg aaccagacct gctgaaggcg ggggctgtcg tgcggcttcg
     2461 ggtgaagcca gggcatcttt tcggcgggat gctcctgccg gagcccctct tcggcgagtt
     2521 catcccacag caaagggtgg aaggcgtcac gctcgtcgtc gatggcgagc gcgtggcgcg
     2581 cgcagcgcac gtgcgggctc agctcataat tgggcatgcc gagcggaaag atccaattgt
     2641 cgatgccgcg cgtgatctcg gcaatcgggc cgccataggc ggcgacggtg tcccacactc
     2701 cgacgaaacg gatcaccgga tgatagtttt cggcgcgcag ccgctcgagg aggtcctgat
     2761 ccttccgtgc gcgattgcgc ctgaacaatt tgctgtacaa gttccaaagc gaccagccac
     2821 tgcggggcgc gcagcccgca cggtacgcgc gccacgcctc cttcgatcgc tgatggagat
     2881 cggcctcgcg cgtggtttcg agcaggccta ccttcgcgat cattgcgatg gcggtgcgca
     2941 tcgtgaaggc gccgcggctg aacccgaaac cgtaaatatg gtcgccctcg gcgccgtcct
     3001 gttccgtcgc ctgaagcggc gacccggggt tgtagttccg gcatgcgtag cgatagatat
     3061 cgaggacatt ctgtcgcagg ccgatgccaa agacgccgcc gaggattgcg agcggcttga
     3121 accccgatgt cccgaccccg ttgtcgtaga aggcgatctg ttttcgcttg ccaggagtgg
     3181 ccggcccaag gtcgaccgcc tcgtacatgc gccagacgtt ggtcttgaac agtttggcgc
     3241 tgctgttgcc ggtgccgtcg gagaagagga tgatcgactt ggctgcgccc gcgccgcccg
     3301 cgtggccggc tggagccgct ccgttcggcc cggtcttcgc ggcgtcgccg gccgctgcct
     3361 cgtttcgctg gtcgcccatc gcgttcccct cgcgaagaag cggtgagggt gcgcctcccc
     3421 cagggtgtcg tcaaccgggc gcggcggcgg ggggcgggaa atgactccgg aatcgatccg
     3481 gtcgcggcgg aagcccccgg cggcgcgcga gccggcttgc gcggtgcggc ggcttcgata
     3541 ggttgccggg cgtaatgaga cccgttcggg ctgcccgccc cctcccggct tcggtcgcac
     3601 tgctgctcgc cctgctcgcc gcggggtgcg cctccaaacc ggagctgccg gcggccgggc
     3661 cgatcgcccg ccccgccccc gccgcctacg atctcgtgat ccgcggcggg acgatctacg
     3721 acgggagcgg cggcgcgccc tataagggcg atgtcgcgat cagcggcgac cgcatcgtag
     3781 cggccggtcc cgcgctgaag ggcgcggcga agcgggagat cgatgcgcgc gggctggccg
     3841 tggcgccggg gttcatcaac atgctgagct gggccaacga gaccctgctc caggacggcc
     3901 gcggcgagag cgatatccgt cagggcgtga cgctcgaagt gatgggcgag ggccgctcga
     3961 tggggccgct caacccgcag atgaaggccg agctccagaa ccgccagatc gagatcaaat
     4021 atccggtcac ctggaccagc ctcggccaat atctggaaga gctcgaggcg cgcggggtca
     4081 gccccaacgt cgcctcgttc gtcggcgcgg cgacggtgcg gactcatgag cttggggaag
     4141 cggacgtcga tccctccccc gagcagctcg ggcgaatgcg ggcgctggtg cgccaggcga
     4201 tggacgaggg cgcgatgggg gtcggctcgt cgctgatcta cgtgccggcg accttcgccg
     4261 agaccgacga actggtggcg ctggccgccg aagccgggcg gtgcggcggt atgtatatca
     4321 gccatatccg gaacgaaggg ccgcggctgc tcgcctcgat cgacgaactg atcgagatcg
     4381 gccggcgatc gggcgcacgg gcggaagtct atcatctgaa ggcggcgggc ccggccaatt
     4441 ggcatctgat ggacgcggcg atcgcgcgga tcgcggcggc gcgggcgggc gggctgccga
     4501 tcaccgccga catgtatctc tacaccgcct cgggcaccgg cctcgacagc acgctgccga
     4561 tatgggtgcg cgaaggcggc catgacgcga tggtggcgcg gctgaagcag cccgaaatgc
     4621 gggcgcgggt ggtggcggag ctcagggggg cgcggccggg gcgcgactgg tccgcggtcc
     4681 agccgagcag cttccacaac ccggcgctgc gccactatgc caacaagcgg ctgagcgaga
     4741 ttgccgcctt gcgcgggcag agcccggagg agacggcgat cgacctcgtc gtcgaggatg
     4801 acagccgggt cggcacgatc ttccattcga tgtcggagga caaggtccgg ctcgggctgg
     4861 tgcagccgtg ggtgagcttc ggatcggacg cgggcgcgat cccggccgaa ccgcccttca
     4921 ccgacacgcc ggtccacccg cgcacctacg gcaatttcgc gcggctgctc gggcgctatg
     4981 tgcgggagga gaagctggtg acgctggccg aggcggtgcg gcggctgacc tcgctgccgg
     5041 cgacgaacct ggggctgagc gaccgggggc ggctgaagcc gggctatttc gccgacctgg
     5101 ctatcttcga tccggcgacg gtcggcgacc gcgccactta cgagcggccg caccaatatg
     5161 cggtggggat gcggcatgtg ctggtgaacg gggtgccggt gctgaaagac ggggcgcata
     5221 ccggtgccag accggggcgg gtggtgcgcg ggccggggtg gaggaagtgc cgggcggggt
     5281 aggtttgccg gagggaacag agccccggtt atcgccgggg gactgggccc cggccttcgc
     5341 cggggaacgg cacctgcttt ctcccgggga cggatagggc ttggcggggt gctacatatg
     5401 tggcatgcta ctagtgtagc agggtgagtc gcgatgatcg ggagccttcc gcgccgcgag
     5461 cgcgagatat tcgaattgct ctgcagcgcc ggcgaggcga cggcggcgga cgtgcgcaag
     5521 gcgatggccg acccgccgag ccattcggcg gtgcgtacga tgctggcgcg gctcgaggcc
     5581 aaggggctgg ttacccaccg cgccgtcgac caggcctatg tctataagag cgtgccgcag
     5641 ccggccaagg tgcgggaatc ggcgctcaaa cagatggtga agacgttttt cgacgggtcg
     5701 gcggcgagcg cggcgaccgc cttgctcggc ctcacccgca accttcggcc cgaggagctg
     5761 gacgcgctcc agcgggccat cgacgaagcc aaggagcggc catgacgagc tttgccttct
     5821 ttgtggaaat ggcctggaaa tcggcgctga tcgccggcgc ggcgctgttg ctggcggggc
     5881 tggtccgatc ctggtcggcg gccgaccgga ccgcgctgct gcggctcgcc atcgtcatgt
     5941 tgctcggcct gccggcggtc gcgttgctgc tgccggggat cgaggtcgag atggcggtcc
     6001 cggccgcggc cgaggccgag ccgctgctgc gctccgccct gcccgccccc gcggccatgc
     6061 cggcggacgc ggcggcgagc gtggcggtcg cgcccgcggc gggacgggac gatccgggca
     6121 tgctggtggc tttgctttat cttggcgggc tgatgatggt ggggctgcgg ctcgccggcg
     6181 ggctgtggac gctgcggcgc tggaccgccg ccgccagccc gctcgactgc cccgaatggc
     6241 aggccgcgct ggcgcgggcg ctggccgatc cggcggcgcc gcgcttccgc ctgctggtgt
     6301 cctacgaagc gccctcgccg ctgagctggg gctggcggcg gccggtgatc ctgctcgatg
     6361 ccgacacgct gcgccgggcg gacgatgccg acgcgatcct cgcccatgag atcgcgcata
     6421 tcgcgcgcgg cgactggccg accctgatcc tgtcgcggct ggcggtggcc ttgttctggt
     6481 tcaatccttt ggtgtggtgg ctggagcggg tgacggtgca ggaggccgag gaagcggccg
     6541 acggcgcggc ggccgggcgg gtcgagccgg cgcgctatgc ccagatcctg ctcaattgcg
     6601 cgcagcaggc cgggacgctg ccggtcagca gcatcgcctc gggctgcctc aggcggcgga
     6661 tcagggcggt gctcgacggc agcttctcgg ggcggcgccg cggctccgtg tggacggcgg
     6721 cggcgatgct cggctgcgcg gcggtggcgg cgccggtggc ggcgacccgg ctggtgccgg
     6781 cgctggccgc ggccccggcc gcgccggccc ggccggtggc gcggattggg ccgctgacga
     6841 tctcgatgac ggaggcgccc gtgcggatcg cggcggcgcc gctgccgccg gaggcgccga
     6901 tgccgctgcc ggccgccgag gcgccgctgc cgcccgaggc tccggcggtg gcgagcgccg
     6961 cgcccgaggc cgctcttccg tcgcccccgg ctccggtggc tgcccccgcc cctcccgcgc
     7021 cccctgcccc tcccgcgccc gccgctccgc cgcggccgcc cgcgccggtg ctcgacgtcg
     7081 agaccttggt ccagatgcgg gtccatggcg tcaccggcga ctatatcgag gcgctggcgg
     7141 cgatcgggcc gcattaccgg cggctgacgc ccgagcagat ggtgcggatg cgcatccacg
     7201 gcgtgacgcc cgattatgtc cgcgacctcg ccgcctccgg ctatccggcg atcggcgccg
     7261 agcagctgat ccggatgaag atccacggcg tgaccccggc tggcgccaag cgggcggcgc
     7321 tggcgccggg ggcgcggccc tcggccgagg agctgatcga ccgcgccatc caccgcaagc
     7381 tgtgaaaggc cccttcatgt tcgtccgcac cgtcctcgcc ctcgccgccg cggcgttggt
     7441 gcagaccgct tcggcgtcgg cggccgaggc gtccttcccg atcggctgga cgatgtcccc
     7501 ggcggagggc cgaccggcga tggtgcagct tgagcttagc taccgctccg gcggcagcgt
     7561 cggcaacacg tcgcggccgg tggcggtcgc cgatcttcag ggcctcggcg aagcgcagct
     7621 cgccgggccg ggcgggccgg ccgcgttccg catagtccgc gacgccggca ccatcgcctg
     7681 ccagggcgtg gtccgccagc ggcgcgggac cggcgagtgc cgcttcgccg gcgacccgcg
     7741 cttcgccgcc gagctcgccc ggcgcggcat cgggcggccg agcggctatg agcaattcca
     7801 gctggcgctg cacggcggcg accttgcggt ggtcgccgag ctggagcggc agaaatatgc
     7861 gcgcccgacc gtcgagcagc tggtcgggct cggcatccac ggcgccgggc taagctacct
     7921 gcgagcgctg gacgcggccg gctatcgggt cggttcggcc gacaagctgg tcgagatgcg
     7981 catccacggg gtcagcgccg attatatcgc ggagctccgg acgctcggct tcgcgcggcc
     8041 ctcggcggac caattggtgc ggatgcgcat ccacggcgtc tccgccgatt atgcgcgcga
     8101 gctggcggcg ctcggctaca aaaggctgag cgcggaggag ctggtcaaca tgcggattca
     8161 cggcgtgaag cccgagctgg tgcgcgagct ggcgcggctc ggctatcgcg gcgtcaccgg
     8221 cgagcagctg acgcggatgg cgatccacgg cgtgtcgccc ggcttcgtgc gggagatggc
     8281 ggcggccggc tatcgccagg tcccggtcga ggacctcatc cgcctgcgca tccacggcgt
     8341 cacgccggag gaggcgcggc gggcgaatgc ggcgttccgg cggggggatt aggaaaaaga
     8401 gattccagct tgcgctggaa tgacgggtgg gcgcaggttt cggttatgaa gaaacttgtc
     8461 atcgccgccg ccatggccgc cgcttgggtc gctccgttga ctggagcaca gccggcgagc
     8521 gagccgccgc ccgccgcgcg aaccgccgcc gaaggcaatg cggaattcgg tgtggcgctg
     8581 taccgcaagg tggcggagag gccgggcaac gtgttcctgt cgccgatcag catcgtcggc
     8641 gcgttcgggc cggtcagcgc gggggcggag ggggagacgc tggcggagat cgcgcgggcg
     8701 ctgcggctgc cgggcggggc ggcggggcat gaagagcttg gagcgatgct gcgcgggctg
     8761 gagcgcgagc gcgagggcgt cacgctcagc atcgccaacg cgctgtgggt gcagcagggg
     8821 ttcggcctca agcccgcctt cgagaagacc gcgcgcaagc aatatgacgc gcgggtcgag
     8881 gcgctcgact tcgcccggaa cgagcaagcg gcggcgcgga tcaacggctg ggtaaaggag
     8941 gagacgcgcg gccggatcgg cgagctgatc aacgccaggt cgctcaatcg cgacacagcg
     9001 ctggtggtga ccaacgcggt ctatttcttg ggcgattggg cgacggcgtt caggaccgcg
     9061 gcgactgcaa acgagccttt ccacctgccc ggcggcgaga ctcgaacggt gccgctgatg
     9121 tcgcggcagg acccgttccg ctatttcgag accgacgaat tccaggcgat cgaccttccc
     9181 tacaaggatg agcggctgtc gatgagcctg ttcctcccca aggcgcggga cgggctgccg
     9241 ccgttcgagg cggggctgga cgcggcgcgg ctgcgcgaat ggctcgggcg gctcgacgcg
     9301 gagcggccgc gcgcggtgcg gctgcacctg cccaaattgc ggatcggcga gacctacaat
     9361 ctgatcccgc cgctcgggga aatgggcatt gccgccgcct tcacgcccgg ccgggcggac
     9421 ctcagcggca ttgccgaccg ggagctgttc atcagccagg tgatccacaa gaccttcctc
     9481 aaggtcgacg agaaaggcac cgaggccgcc gccgcgaccg gcatcggcat ccaggtgacc
     9541 agcgcgccgc cgccgccgcc catcttccgc gccgaccatc ccttcttcct ggtgatccgc
     9601 gacaaagcga gcggcgcgat cctgttcctc ggccggatcg tctcgcccga ccaggcctga
     9661 gccgacgggc gagccttcgc cgaacggatt cttaccctcg tcgaaaacgg gtgtcagcgc
     9721 cgccgcgggt cgggcggagc ggtgcgatca acaggtggaa gcgggggtaa gcatggaccg
     9781 actgaagaca atcgcgctgg cgctgctgct ggcggcgccg ggcgcatcgc tgcaggcggc
     9841 gccggccgag gtcgcggcgg cggtggcggc gaaggagcgg ccggccgagg ccaccaagct
     9901 cgacgaaagc cgcaagccgg ccgaggtgct cggcttcctt gggctggaac gcggcgaccg
     9961 cgcgctcgat ctgttcaccg gcagcggcta ttatgccgag atcatggcca aggcggtcgg
    10021 gccgcaaggc tcggtggtcg cctgggagcc ggccaatttc tacaatgacg aggtcaagaa
    10081 ggtgtgggcg gagctgcccg gccgggcgcc gaacgcgcgc ctgctggtcg aagccgcgga
    10141 cgcgatggcg ttgccggcgg cgagcttcga cttcgtgctg atgcacatga tctatcacga
    10201 tttctattgg gagagcgccg agtataaata tccgcggctt gaccccgatg cggtcctgaa
    10261 gacggtgttc gccgccacca aaccgggcgg gatcgtcggc gtggtcgacc atgtcgcgga
    10321 gcccggcggc gatacccgcg ccgtggtcga caagctccac cgcatcgacc cggccgtgat
    10381 ccgcgccgat ttcgagcgcg ccggcttcgt cttcgacggc gaatccgagc tgctccgcaa
    10441 tcccggcgac gaccgctcca agctggtgtt cgacccggcg gtgcgcggca agaccgaccg
    10501 gatcgtctac cgcttccaca agcccgcctg agcggcggcc tcagaccgcc ttgtcgcgct
    10561 ggcaggcctc gctgaaggcg gcggcgagga tgccggtcgg catggcgatg aggccgatgc
    10621 cggcgatcgc gacgaaggcg gcgaccagct tgccgagggg ggtgacgggg aagacgtcgc
    10681 catagccgat cgtggtcagc gtcaccactg cccaccacag agcgcgcggg atgctgccga
    10741 acttgtcggg ctggatctcg ccctcgatcc agtagagcgc ggtggcgccg aacacgacca
    10801 gcatcaaggc gaggccgacg gtggcggcaa gctcgaaccg gcgcgacagc accgccgagg
    10861 cgaggttgct gagcgccgcg ctcatccgcc cgagcttggc gaggcggaag atgcgcagca
    10921 gccgcaccag ccgcagcatc gccccgctcg cgccgatgaa gggcaaaagg gccgagagga
    10981 tggcgagcag atcgaagatc gccgccggcg aacggacaaa ggcgagccgg cggcgccagt
    11041 gcgagcgcgc gccctcctcc tcgaccacgg tccacagccg cgcggcatat tcggcgagga
    11101 agaacaggcc gaagccgagc tcggccgcgt cgaacagccg ttcgcggccg gcggtgacga
    11161 gcggctcggt ctcgacgatc gccgcggcgg tggcgacgat gatggcgagc gtcaccaggg
    11221 tgttgagcgg cgacaggccc ggtttggtcc aggccctggg atcgagctgg cggtagagcc
    11281 ggcggcgcag tgacggggga gcaggcgatg gcgggacggc ggccgccgca gcgccctcgg
    11341 cgttcagctg caacggcaat tcgttcatgc cggaagctcc cgattttccc cgcaccccgt
    11401 gtcccaaact ttccgccggt ctgcaacccg cggggtctcg ccgagggcgg aacggcgcgg
    11461 ggcgctcggc cgtttattcc ggcgagggtc cgaagcccgc catttccacc gaaggaggag
    11521 ccttatggca gacgatcgct attcgcagga gcgcggcggt ggccgccgcg attcgatgtt
    11581 caacgacgac gagcgcgaca gctgggaacg ccatcggcgc agcgagatgg gctggggccg
    11641 cagctcggag ggcggcggcc atgaaggcgg cggcttcttc gagcgggcgg cgggcgaggt
    11701 ccgctcctgg ttcggcggcg acgacgacgg gcaacgcggg gacgggcagc gcggcggcgg
    11761 cggttccggc ggcgagccgt ggggcggcgg cggctgggac ggcaacgaag gcgtcggggg
    11821 gcgcagccgg ccgagccatt atggcagcga gcatggctat ggcggcttcc agggcgatta
    11881 tggcgcgggc gggggccagg gcggcggcgg cggccagggc gggttcggcg gccggggcga
    11941 ttatgcgcac ggccggcaga gcttcagcgg cggcgaccgc gaaggcgcct atggcagccg
    12001 cgagggcagc tatccgggcg cacaggggca gtatcgcggc agcggctcgt cgggctggtc
    12061 gaacaacgat ttgagcccgc atccggacga tcattatcgc agctggcgcg aacggcagat
    12121 cgccgagctc gaccgcgatt atgaggaata ttgccgtgag cggcagcaaa gcttcgaacg
    12181 cgagttcggc agctggcggc agggccggca gggcggcggc gcgatgagca gcgggctcag
    12241 cggcaatatg tccggggtgg ccttgggcgg caccgcggcg accggaccga tgggatcggg
    12301 cggcctcacc ggcgcctcgc cgggggcggg cgacttcgcc gccgcggacg agagcggcgc
    12361 cggcgcgggc ggcggcggat cgccggccgg gacgcccgag gctgccgaga gcggcggctc
    12421 ggcgcgcggc ggcggccggt cctgaccaat ggggccgccc ccgtgcggcc ccgctttcca
    12481 ccagcaacga ggagtatgac gatggctggc tatgaccgat atgacgacga cacccatgcg
    12541 cgcagccgcg actatgaccg cgagcgcggc ggcgggcgcg gctttttcgg cttcggcggc
    12601 ggccgggagc ccgagcgcgg cggcggctgg agcggcggcc gcgaccctga gcgcggcggc
    12661 ttgggcggcg gccgcgagcc cgagcgcggc ggcggcttct tcggctccgg ccgtgacgag
    12721 catcgcgagc gcggtggcgg cttcttcggc ggcggcggcc atgagcgcgg ccatgagcgc
    12781 ggaggcgagc gcgattacgg ccgcgacgat catcgcgcgg gcctgccaat cgaggagacc
    12841 agtcggctca tcgggtccaa caaggtcgaa ggcaccgcgg tctacggccg cgacgggcat
    12901 cggctcggca gcatctacaa tttcatggtc gacaagattt cgggccgcgt cgaatatgcg
    12961 gtgatgacgt atggcggctt cctcggcatg ggcacgcgct attacccgct gccgtggcgc
    13021 gccttgagct atgacacacg cgccggcggc taccggatcg agctcaccga acgcgacctc
    13081 cagagcgcgc cgagcttcga ccgcgacagc gagccgaggt tcgaccgcgc ttatggcgag
    13141 cgcgtgcacg attattatgg gctgaactat tgaccggagc cgcggccgtt cgccccggcc
    13201 gcgcccctac ggcttattag tcttgtttgt cagaggcttg gcaaggtgag gccgttgctg
    13261 taagggcccc gccgtaaaag gcaggggatc catgagcaag aacattccga tcgcgtcggc
    13321 cgcggcgctc gcactcgcgt cggccgcgcc ggcccagccc ggcggcagcg ccgagaccgc
    13381 ggccaagatg cacgaattca gggcggctaa cgtgccgctg gtcgcgcagc cgctcgacct
    13441 gtcgcagaag agcgacccga tctgggtcaa gggcgagcgg cccccgggca acgtcatagc
    13501 gcggctggcg ctgaagcccg tcgaagcgat cgtggttgcg gaagatgtcg gccagggctc
    13561 cggccaggcg ctgctggcgg cgctggcgct ggccggcgcg gccggggcct acagccggac
    13621 cgccgacctc cagggcgcca gggagcagcg catctattgc ggcgccaccc cggccggcac
    13681 gttcaagatc ctgtgcctcc aggaccaaga cggcgacgga cgcctcgacc acgcgatgcc
    13741 cggggccgcg gaagccgggg tggcgg
//
LOCUS       DASVRK010000112        33556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_206585, whole genome shotgun sequence.
ACCESSION   DASVRK010000112 DASVRK010000000
VERSION     DASVRK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 33556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33556
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206585"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VF589_03995"
     CDS             complement(<1..900)
                     /locus_tag="VF589_03995"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX8256771.1"
                     /translation="MADIPARTELDPPRNGEGDHAKHGGGAAAQLAVTTGPIRGSRKV
                     HVGPLAVAMREIHLEPGCGEPPLRVYDPSGPYTDAAATIDILAGLPELRSTWIRARGD
                     VEEVPQREVKPEDNGQLGPDRSGGVAPFPNVRKTVLRARPGANVSQMHYARKGIVTPE
                     MEYVAVRENLGREQLAAQIRDGQDFGASIPDYVTPEFVRDEVARGRAIIPNNINHPES
                     EPMAIGRNFLVKINANIGNSAVASDVAAEVDKMVWSIRWGADTVMDLSTGRNIHDTRE
                     WIIRNSPVPIGTVPIYQALEKVGG"
     regulatory      complement(927..1039)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1089..1520
                     /locus_tag="VF589_04000"
     CDS             1089..1520
                     /locus_tag="VF589_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX8256772.1"
                     /translation="MDRRQFEETLNQLPIGRDPASVRRRMEAMERVLERAFVIPGINR
                     PVGLDSIVGLVPVLGDVVTASMGAWLVWEARNLGMSKFQLVRLAGNVGVDTAIGAIPL
                     VGDLFDFVFRSNTRNLRIIKRWLDKNHPETMVIEGEVVARP"
     gene            complement(1560..1982)
                     /locus_tag="VF589_04005"
     CDS             complement(1560..1982)
                     /locus_tag="VF589_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256773.1"
                     /translation="MRPKSIILFERVVAASLVLGLLGIFLYWENSAAAIRQVGLGTGF
                     LAGVMLITFGLYFLLLWLIARRRSRVAAWIYIVLTGLSLVTGLIGIPAMLQGQPLHIA
                     LTVVQYGLNFLSVLLLFRRDSRDWLAGKDNPQYDRVFD"
     gene            complement(2051..4147)
                     /locus_tag="VF589_04010"
     CDS             complement(2051..4147)
                     /locus_tag="VF589_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8256774.1"
                     /translation="MLRLAIPAAALLLTAAAPPAASPEDPHLWLEEIEGERALTQVKA
                     WNKATEDQLTRAPRFEDYRRRAREILDDERQIAAPEAVIGDHVTNLWRDADNPRGLWR
                     ISPLAAYAAGKPQWRTLIDVDALGKAEGKSWVWHGADCLAPEHARCLVSLSPGGTDAD
                     VVREFDLKAGKFVEGGFTLPEAKTNVAWVDRDRLLVITDYGPESLTTSGYGRIAKIWR
                     RGTPLASAATIFTGAKEDVQVSPIAAVDRDTRWVMLNRAKTFWTSDLSLLTQDGKLVR
                     TPLPETAEYEDVIGGRLIAKLNRPLGDIPAGAVVAYSLADIAAGRAPAPELVMAPSRS
                     QAIEEVAASDTIVWIKALDDVSGKLFALTRGADGRWTSRAMPLAANSSVHLVGVAGKQ
                     DLAFATVEGMLTPPALYSVRPAATPVLAQSLPARFDATKFAVEQRFATSKDGTRIPYF
                     LVRRKSGGKASVPALIHAYGGFRAAQTPTYLTGQPYRAGPLGLFWAERGNAYVLANIR
                     GGGEYGPAWHESVLRENRQKSFDDLHAVAEDLVRTGVSQKGKIAISGRSNGGVLVGAA
                     LTQRPDLYGAVISGSPLHDMKRYSQLLAGASWMDEYGDPDKPADWAFLSRYSPYQNVK
                     PGVKYPPVFFYLSTKDDRVHPGHARKMAARLGEYGNPFYYHEFLEGGHSVGADHAEDA
                     VRAALLTTFLEKELGG"
     gene            4268..4522
                     /locus_tag="VF589_04015"
     CDS             4268..4522
                     /locus_tag="VF589_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX8256775.1"
                     /translation="MAHYSVAAAKDALSSLIAKAEAGEEVIITRHGKPAVELRPAQSD
                     SRDRKRAMEKLRAHRQNMAPLTTPIPYHRFSEWLYEDEES"
     gene            4519..4992
                     /locus_tag="VF589_04020"
     CDS             4519..4992
                     /locus_tag="VF589_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8256776.1"
                     /translation="MSFYLDNSVLVPLFVAEARSEAIQHWLEECERPVHVSDLAISEF
                     NATMSGLVRTKDYSREQARAQAAAFERWRLEFAEPTENLPVDVRLASKLVAEPFPKLL
                     TPGAIHLATCKRMELTLVSDDKRLIEIAGRLSVDVISPPSIRVEEEPQPRLKPPR"
     gene            complement(5326..8799)
                     /locus_tag="VF589_04025"
     CDS             complement(5326..8799)
                     /locus_tag="VF589_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256777.1"
                     /translation="MAVPTNGDDILVGTSGPDIIDGLGGDDRISGEEGNDQLIGGSGN
                     DYLDGGLGNDDLQGGIGNDRLFIAGAGTDAAAGGSGFDMLEIDWRDSAHALRNNGAVT
                     ANPAGGFDGGFTNFVDRSVNFTGIERFSIMSGAGADDFTTGGGNDNVATGLGDDHVNV
                     GRGKDVANGGGGTDGLSADWSQQAAGVVVDLRNDLNSGGYGSFRSFEYFGTVFGSAHD
                     DVLTGSAVARNETFNLGGGNDTARVFHGHDAVHGGAGIDTLAIDHSASPHAFRNELAS
                     TAGADGFSGKFSNFNDRSVSFTGIDRFDIVTGSGADDFATAGGDDVVSSGGGNDHVNV
                     GAGVDTADGGDGIDGLSADFSADAARVVIDLRNETNEGVFGSFSRFEFFGTVIGSRFA
                     DSLIGTGSASNDAFLLGAGNDLAGVFHGHDNVAGGSGRDTLMLDYSASGHALRVDTEL
                     TGDAVSGHSGKFSNFVDRTVAFSGIERFVITSGSGADIFRTGTGNDVIASGAGDDLIN
                     VGSGKDIAHGGADVDGLSADWSGQTEGVVIELRFAVNSGAYGSFTSFEHFGTVNGSSH
                     ADTLIGTTASRTDIFNLGGGDDIAGVVNGHDQVHGQGGLDTLAIDYRSSGHGLRNEGA
                     ITASDGSGFTGKFSNFVDRSVSFTGIERFDIKTGDGADIFATAAGDDVVASGGGDDLI
                     DVGSGFDTADGGAATDGLSADWSVQAAGVVIDLRNLENSGGYGAFRSFEHYGTVTGSS
                     HDDRLIGTNAARTDIFHLAAGADYAGVFHGIDTVNGGTGSDTLMIDYRASGHALRDET
                     AKSEGVDGQSGKFSNFVDRSVNFTGIEHFEIYSGSGADNFSTGSGNDLVFTGIGDDHV
                     DVAGGVDQANGGTGVDGLSADFSGDDRGVVINLTRGVNSGAYGTFTSFEYFQNVVGSG
                     FADRFDGTDFARNELISLGAGNDVATVRHGLDTVHGGSGFDTLVIDYGSSAHKLQIEV
                     NYSSNPGGGVDGKFSNFVDRSIAFTSIERFIFTLGSGNDNVAGGNATDNLNGGAGNDV
                     LNGGNGNDVLTGGMGTDSLTGGGGADIFVYIDQAESGPGAARDKISDFVQGADRIDLS
                     DLDADSTTPATEDFTFIGTAAFGSVAGQLRYTAAGGITVIEGDTDGNGLADFQIELTG
                     EIPLQSTDFIF"
     gene            8957..10324
                     /locus_tag="VF589_04030"
     CDS             8957..10324
                     /locus_tag="VF589_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8256778.1"
                     /translation="MESNGKSTAKERLRSWKEIAGFFGTDERTVKRWAATRGLPVQRV
                     PGGTRTPIYADVAALQNWLAASHVGAAETIVPPAEPPPPAAPARRARSAPLAALAGAA
                     VAAAIALALYLGPFRDGEASAHQPPQEAVELYLAGLLHWEKRSPESLARATRYFSQAL
                     ARDPEYAEAYAGLANCYLLMREYTGMPDDQAYPMAEAAARRALSLDPGLSQAHAALAF
                     TTFYWARDFERAQQSFKRAVALDPASAHARHWYATALLHVGDMKGALEQINAAQRLAP
                     QSASILADKGLVLFHAGRAPEAMAMLREVSALEPEFQSPHVYLSIIQLAGGEDAEFLR
                     HARHAARLRGNEARLALLDDAQRGFEQGGRRGMLEAMLAAQMRLHAEGRESAYAVAAT
                     QALLGDRAQALRYLRKSVEEREPLAVGLRIEPAFKGLRGDAGYQALAGRVGNPAAKSR
                     RVVAG"
     gene            complement(10737..11738)
                     /gene="nadA"
                     /locus_tag="VF589_04035"
     CDS             complement(10737..11738)
                     /gene="nadA"
                     /locus_tag="VF589_04035"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX8256779.1"
                     /translation="MNAPNLSLRGLDIAAEIDRLRKERNAVILAHYYQEPALQDIADF
                     VGDSLDLSRKAASTDAEVIAFCGVRFMAETAKILSPEKIVVLPDMNAGCSLEDSCPPD
                     QFKAFREAHPDHIALTYINCSAAVKALSDIIVTSSSAETILDQIPKDQKIIFGPDKNL
                     GGYLVRKTGRDMLLWPGVCIVHEAFSETELLKLKAQHPGAPVLAHPECPPHILDHADH
                     VGSTKAILEYALSSPSEVMIVATEPHIIHQMEKAAPHKTFIGAPGADGNCNCNMCPYM
                     ALNNMEKLYVALRDLQPRIEMDEDLRLAAKKSLDRMLDMAGRTVGQGDVGRPVFARA"
     gene            complement(11762..12388)
                     /locus_tag="VF589_04040"
     CDS             complement(11762..12388)
                     /locus_tag="VF589_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX8256780.1"
                     /translation="MGAVRLGGLFGGGPDPETIAVASLQSMREQARLTPFAARYVAVV
                     TSRQTRFGLTAQKTLIMPGNVRYEVDLAKLQQRDLAWDEPSKTLTISLPLLEISGPDI
                     DLSQVREYDQGGVLMALTDAEKALDSANRQRGRQELVRQAREPLPMRLARDAARRAVE
                     RSFAMPLRAAGLDAQVVVRFADEAGEDPSRLDRSRRMEDVVKERQGSR"
     gene            complement(12448..13308)
                     /locus_tag="VF589_04045"
     CDS             complement(12448..13308)
                     /locus_tag="VF589_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8256781.1"
                     /translation="MAEMDAPGTIERLHPLVRRVLAPNPSPFTFTGTMSYIVGTGEVA
                     VIDPGPAIAGHVDALLAATAGERIAAIVCTHTHRDHSPASRPLQAATGASIVGCAALA
                     PPDDGFGADEAFDADYAPGRTLADGDTLAGPGWTLAAVATPGHTSNHLCFALPEAGAL
                     FSGDHVMGWSTSIVSPPDGDMGAYVASLDRLLARHDSVYYPGHGPAIEQPAKHVRGLI
                     LHRRHREAQILARLGEGQGTVPAMVAAMYADIDPRLHPAAERSVLAHLIDLAARGRVA
                     RRGEAWERAA"
     gene            complement(13367..15259)
                     /locus_tag="VF589_04050"
     CDS             complement(13367..15259)
                     /locus_tag="VF589_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX8256782.1"
                     /translation="MTAWALDTLIGVTLLLLLVLAVRRPVARAFGAGWAYALWLLPLL
                     RLFMPPVELLGGAMAEALPKYTVIMLPAAAAAGEAAAAAPAAAGLADWLPLLLALWAG
                     GAGAFVMWQQSAYSAFLLSLGTNCRPGDPPIHGGVKVVESGAVDGPLAVGLLEKRIVV
                     PLDFAARYSAAERGLALDHELVHHRRGDIFWNYVALALLALTWFSPVSWIAFRAFRTD
                     QELACDAAVTARIAPDRRLDYAFAMVKSASRPGEVAACPMNRADQLKRRLMMVKQHRS
                     SRLRSLAGLVSVAILGVAGLALSSPGFAQQDDGAAAPQTLVSRGGDIAPVVGARDRAV
                     LAAKCAPGGYENPPRYRSGAAVGRVILCSDPAAASDPQVQAIVARSIDRARVELAAAR
                     PEPGLADRVAETAVRYAALAVEPFDRIYDDEFDDAHAARERAHALREAAHARSEAAHA
                     RAEAAHGRAEALREAAHARGEAAHARAEALRERGEALRERGEALREAAEARAEAARDR
                     AEALRDRAEALREAAEARAEAQRDAAEAAREAAEARAEAAAEARAQGLVHARAGVKLA
                     RASLQRAHGPARLHVRGPLSPAGLNPRELKALQAEMQRARAEVRRADIEIDREMGELH
                     AELEIN"
     gene            complement(15256..15621)
                     /locus_tag="VF589_04055"
     CDS             complement(15256..15621)
                     /locus_tag="VF589_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX8256783.1"
                     /translation="MERIGDAEFAVMEVLWDEAPLTAAEVAERVPAERDWNVRTVKTM
                     LSRLLAKGALAHEEDGRRYLYRPAVRREDYVAQESGKLLDRMFGGRLTPLVAQLAERD
                     RLSAEDIAEIEALLKALKP"
     gene            complement(15689..16201)
                     /locus_tag="VF589_04060"
     CDS             complement(15689..16201)
                     /locus_tag="VF589_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256784.1"
                     /translation="MDEEGPILSQTDEAMIRAKCGTGPARNSGIQFNGGALVCSNGRR
                     VDDPEVRALAARIDSRVKAHLDRVMRRPEVKAALNGEVDARVRAALRQTDAELAKVDG
                     AVARAVAAAAVAERHAGLAAAHFGPAERARLRAELGRVHAEIDAIDFEAIGRDLEAAL
                     DGARRSRESH"
     gene            complement(16867..17193)
                     /gene="grxD"
                     /locus_tag="VF589_04065"
     CDS             complement(16867..17193)
                     /gene="grxD"
                     /locus_tag="VF589_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX8256785.1"
                     /translation="MSDNNARIDEIVKSNDVVLFMKGTALFPQCGFSSRAVSILEHLG
                     VSFETVDVLQDQEVRQGIKAYSDWPTIPQLYVKGEFVGGSDIMMEMFEAGELQQLVDE
                     KQVAKA"
     gene            complement(17205..17438)
                     /locus_tag="VF589_04070"
     CDS             complement(17205..17438)
                     /locus_tag="VF589_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX8256786.1"
                     /translation="MPMAADRIEALIREGIPDARVEITDLAGDGDHYAARVVAESFRG
                     VPKVKQHQRVYQALGGRMGGELHALQLTTATPE"
     gene            complement(17438..17764)
                     /locus_tag="VF589_04075"
     CDS             complement(17438..17764)
                     /locus_tag="VF589_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEX8256787.1"
                     /translation="MTTFDDREKAFESMYARDQEMQFRVIARRNRLLGAWAANLMGLT
                     DVEADSYAKDVVRADFEEAGDEDVIRKVLGDLTSAGVEIDEARIREALEHKTVEARRQ
                     MIEAQS"
     gene            complement(17834..18832)
                     /locus_tag="VF589_04080"
     CDS             complement(17834..18832)
                     /locus_tag="VF589_04080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8256788.1"
                     /translation="MKALMSNEPGGPETLRLAELPDPVPSKGQLLVRVRACAINYPDV
                     LIIEDKYQFKPPRPFAPGGEIAGIVEAAGEGVEGWAEGDRLIAMLGHGGLAEKVLVDA
                     RQALPLPAGRSFAEGSALILTYATSIHALLDRGRLQEGQTLLVLGAAGGVGLAAVELG
                     KAYGARVVAAVSSEEKAEAARAAGADAVTVYPRGPFDKDQSKALAQQFKDAVGANGAD
                     VIYDPVGGDYAEPALRAIAWEGRYLVVGFPAGIPRLPLNLTLLKSCDVCGVFWGAFAA
                     RDPKANAAHIRTLFRLWEEGSIAPRISRTWPLAEAGEAIAHMAARRAVGKLVVTVD"
     gene            19103..19582
                     /locus_tag="VF589_04085"
     CDS             19103..19582
                     /locus_tag="VF589_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256789.1"
                     /translation="MTIAELAAEPRRYKGRCVAVTAPATHWSLFSSTPSLERQVRSSR
                     SALVRRSERRRQVGWYHPPGELDPQVELGLAKGILLSVRGIVDDCHTLRERELSEAKA
                     EYRRELAARPREPAPPPSDEDRLVEEGEPSAVFLFGYCHWNPGTIIRAVAVTPATGR"
     gene            19633..20130
                     /locus_tag="VF589_04090"
     CDS             19633..20130
                     /locus_tag="VF589_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256790.1"
                     /translation="MAYGLIMKAAALPAVTLPQLSSALEASWDRRTAYLGACRPANPA
                     LGQCYPTSRVVQWFFPRLEIASGEVDTGSGFEAHFWNVDPASNPARHVDLTWRQFAPG
                     SKVARFEILDRDALNDTPPTIARCELLLSRVLLKLEAAGAIDRTGGAASMPALGGHPP
                     NVRDG"
     gene            20163..20690
                     /locus_tag="VF589_04095"
     CDS             20163..20690
                     /locus_tag="VF589_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8256791.1"
                     /translation="MSDKYEPPSEFLKTLIEDDSSLRGDERSDANIRRLIELMRDKDP
                     ANRDWATSLLAGTDADTPEVRGALAAAAEDEHAQTRSEAILGIAQRNPDAALPLLQRE
                     LAGDMVTMATLEAAMLVAHPLLVAGLRRFASPSDHAPIDECIDEALTACEAGKPPADY
                     WADSDPFNIAWRADP"
     gene            complement(20808..21056)
                     /locus_tag="VF589_04100"
     CDS             complement(20808..21056)
                     /locus_tag="VF589_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256792.1"
                     /translation="MHGIETLGDEEIVHVSILDSAGEVEVGHMPFARPAFLRSADQLL
                     ATVAPGDEFRQGYAYWRAEFEKGAAGIYAIDVCEALSL"
     gene            21376..21552
                     /locus_tag="VF589_04105"
     CDS             21376..21552
                     /locus_tag="VF589_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256793.1"
                     /translation="MEGVKAMGNLSTIGGATIWVAVVFALMLAVIEPVSVTSSTGQPA
                     SGAAQGETKAPASQ"
     gene            complement(21735..21811)
                     /locus_tag="VF589_04110"
     tRNA            complement(21735..21811)
                     /locus_tag="VF589_04110"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21775..21777),aa:Pro,seq:tgg)
     gene            complement(22100..22174)
                     /locus_tag="VF589_04115"
     tRNA            complement(22100..22174)
                     /locus_tag="VF589_04115"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22140..22142),aa:Ile,seq:cat)
     gene            complement(22224..23267)
                     /locus_tag="VF589_04120"
     CDS             complement(22224..23267)
                     /locus_tag="VF589_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8256794.1"
                     /translation="MPRGDLDEEAGSGRLDRPPDDRAYLRLPESFGRRFAVFVDTEEE
                     FDWSQPPSRDERSTTAAESLPLIHKRLRGYGIAPTYLVDHPIATDVRCVATLREYQEA
                     GECTVGTQLHPWVNPPFTEELSARNSFVGNLPAEHERAKLACLTDTIEQAFGRRPTVY
                     RAGRYGVGPNTAGLLVEAGYTLDVSVRALFDYSPERGPDFSGIRPLPYRLCGGALLEV
                     PLTSAYVGALRRRGPNLYPRAGRVPHLRGLLARTRLLGRVALTPEGMPVGEVLEAIDR
                     LLDDGLQLFSISFHSPSLEPGHTPYVRTRDDLDLFYAWWEGVTDLFARRGVAPASVDE
                     ILAAAGGARPARR"
     gene            complement(23276..24964)
                     /locus_tag="VF589_04125"
     CDS             complement(23276..24964)
                     /locus_tag="VF589_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8256795.1"
                     /translation="MRFDDRIATVMALPASSASARAAKWRQLVDILAQARTRQGARLE
                     EAFAYLREHRAEIDRATRKEVAMSLTGRAITADLFRFFAEDQPVVAGPLIAGARLAID
                     EWLDLLPSLSPTARALLRHRRDLPPQVVQALASFGASDFVLSGTIGEAPAPRERGSGE
                     GAAGEGALSPGLDKGEAQIRDLVARIEAFRKHRAASPPPDPVEAVVRPVEGFRWETGA
                     DGVILWVEDAPRGPLVGQSIANIAGPAYYGIDGQAAGAFEKRAPFRDARFSVAGDGPA
                     AGDWRISGVPFFDAGSGSFLGYRGTARRPRVDEVARAHGPQMPAGLFGSDLPADSLRQ
                     LIHELRTPLNAIVGFAELIDGQYMGPAHARYRARAGEIMAQAARLLSAVDDLDTAARI
                     ETSRLNLDAGSVDAAALLERLHAAYERVASQRSARLAIEISRGLPPVKAELGALERMF
                     ARMLAATIGLAGEGETIAALMALEPLGGREMLCLAIDRPAAIDGLDEAALLDPGYSPE
                     GDWPGAPALGLGFALRLVRNLAEAAGGALVIGRERFSLYLPAFQAIERSTGQGV"
     gene            complement(25151..26386)
                     /locus_tag="VF589_04130"
     CDS             complement(25151..26386)
                     /locus_tag="VF589_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256796.1"
                     /translation="MKAISAIALAASLAVGAAAIATPAIAQKKQAAASSWTAKLSKAE
                     QAVLAPLEKAVRASDWAAANAALPAAAAAARSADARHFVGQFQYQIGKGTNDKRVEMQ
                     GLDAMVASGGGDPADRAALIKEQAAVAQQTGDNAKAARALAEYLKLNPNDQEMVAALA
                     SMQARDNPAQAAALVEQQIATAKAAGQKAPEDTYKRALQFAYNGKQGPKSVQLSRELV
                     TAYPTPENWRTALLVYEQNGRLDKAAEMDLLRLMRTTKSLTDKANYFRLANALHEKGL
                     PGEAKAVVDEGLAARVYTADDPSFKALGAQTGPRIAEDRAALPGLEQKAMASGTGTLA
                     LNTADAYFGYGDYAKAAGLYRSALQKGSVDANLVNTRLGAALALAGQRAEAEAAFKAV
                     TGARAELAAFWLLWLSQRG"
     gene            complement(26424..27659)
                     /locus_tag="VF589_04135"
     CDS             complement(26424..27659)
                     /locus_tag="VF589_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8256797.1"
                     /translation="MNSVSSIALGVSLALGGLAAAGAPAHAQAGAAAPAVPTLSKAER
                     EALAALQTALQQRNYPAAASAAAAARSAARSGEVRYLAASMELRLALETTDRAMQASA
                     IEAMIESGAAPPAALGELYKNQGILALGARRYDRAEAALTRWVEVQPNNPEALVGLAE
                     IKDDIRKVPEAVTLLDRAIKLRKAAGQPVPESWYKRGLKHAIDAQMPAPSIEFSRGLV
                     ADYPSPENWRDALLTYRDFAQPDPAARLDLMRLMRSANALGGERDYLELAQSFDTGGV
                     PGESKAVLDEGVKAKMVDPAKATYKELIAASGKRATAERKTLASSRTKAMANATGTLA
                     LSAGDMHFSYGEYAAAAELYRAALAKGSVDASVANTRLGMALALAGQRAEAEAALRAV
                     TGPRTHLASYWLLWLAQRG"
     gene            27861..28616
                     /gene="yaaA"
                     /locus_tag="VF589_04140"
     CDS             27861..28616
                     /gene="yaaA"
                     /locus_tag="VF589_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEX8256798.1"
                     /translation="MIAILSPAKTLDYKRPVPPVRPTEPAFADDAAKLARSAARLPRA
                     RLMELMHISKELAALNADRFRGFADQPERAAIYAFAGDVYTGFEVHSLDEEAVLFAQD
                     HVRILSGLYGLLRPLDAIRPYRLEMGTRWAPRRRDLYAYWGPRIAQALDRDVAAQGDL
                     IVINLASKEYWRAVESRLGPGARVIEIDFREEGPAGLRFNTFAAKRARGMMARYICEH
                     RLDDPEALKAFGSDGYAFVPEQSDAQRWRFSRS"
     gene            28739..31504
                     /gene="gyrA"
                     /locus_tag="VF589_04145"
     CDS             28739..31504
                     /gene="gyrA"
                     /locus_tag="VF589_04145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8256799.1"
                     /translation="MASAPPTNDSPTGADITPISIVEEMKTSYLDYAMSVIVSRALPD
                     VRDGLKPVHRRILYACQEGGFVPGRPYRKSAKIVGDVMGNYHPHGDASIYDALARLAQ
                     DWAMRVPLIDGQGNFGSMDPDPPASMRYTEARLAKVAMTLLEDIDRDTVDFSPNYDGS
                     REEPQVLPARFPNILVNGAGGIAVGMATNIPPHNLGEVIRACKAYMDDKEITIEQLME
                     IIPGPDFPTAPLILGQAGARSAYHTGRGSIIMRARHVVEEGRGDRRSIVLTAIPYQVG
                     KNGLVEKIAEAAKDKRIEGVSDIRDESNREGVRVVIDLRRDATPDVVLNQLWRHTPAQ
                     SSFPANMLAIRGGRPETLTLKDIIEAFVKFREEVITRRAKFDLAKARERAHILLGLVI
                     AVTNLDEVVRIIRGSASPAEARAALLAREWPVAEIASYIALVEAVEHEVDGDSYQLSE
                     AQVRAILELRLHRLTGLGRDEIAGELRELATSIGELLDILGNRVKLYEVMRGEFDEIA
                     AQFATPRMTEIAPAWDGIEDEDLIEREDMVVTVTLGGYIKRTPLDTFRAQKRGGKGRA
                     GMATKAEDAVTNLFVTSTHTPVLFFSTHGKVYRLKVWRLPEGAPQARGRPMVNLLPLA
                     EGETISSVLPLPEDEAEWKNLHIMFATAKGIVRRNSMDAFTRVPTAGKIAMRFGESDE
                     SEDMSDRLIGVSLLTEEDDVLLATKCGKAIRFAATDVREFQSRTAAGVRGARLQDGDE
                     VISLSILKGFRASTEERDDYLRHAPWRERAEDAAPPPPRDMSDERFERFRAAEEFILT
                     VCANGYGKRSSAYEYRRINRGGQGITNIGNLDRNGPVVASFPAHNGEQLMLVTDQAKL
                     IRMSVGDTRVIGRGSAGVRLFHVAPGEHVVSAARIEESEEEAEAVVDVEDPMLQASDA
                     VISGAED"
     gene            31964..32515
                     /locus_tag="VF589_04150"
     CDS             31964..32515
                     /locus_tag="VF589_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HEX8256800.1"
                     /translation="MPLIDHPDAKAFILHWGEMGTQWGVNRSVSQIHALLYLSDRPLT
                     ADEIVEQLNLARSNVSTGLKELQGYGIVRRVHVEGDRRDHFLAETDLWDMLMRITAER
                     KRREIDPTLALLGELAARLKQDESAPRHVRERITRMHEFISTLGNWYDQVRLLPKPTL
                     VTLMKLGARVARFIPGAAKPKQP"
     gene            32600..32977
                     /locus_tag="VF589_04155"
     CDS             32600..32977
                     /locus_tag="VF589_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256801.1"
                     /translation="MVEIAYLLYLLISIGITILVARTLSRNGEVFLVQCFGHNETLAR
                     STNHLLVVGFYLVNIGFITLTLSIGAEPTTLPEAIRFLSSKVGLAVLVLGGMHFFNMT
                     GIAHFGRKVSEWFAVQQEARPLA"
     gene            33035..33448
                     /locus_tag="VF589_04160"
     CDS             33035..33448
                     /locus_tag="VF589_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256802.1"
                     /translation="MYGRKPAGRTLMVACLVAGPTFTSTFALANAYLKLPQPILLTVP
                     EIAPLLAFCLAATAFGFVIALVPTGLGTLLMSSLGDHVPAARTPLAWTLAGAAIGYGL
                     AMLTGAWPGLPPAAFALVATSALCARICRGGAEWE"
BASE COUNT         5259 a        11102 c        11906 g         5289 t
ORIGIN      
        1 gccgccgacc ttctccagcg cctggtagat ggggacggtg ccgatcggga cggggctgtt
       61 gcggatgatc cattcgcggg tgtcgtggat gttgcggccg gtggagaggt ccatgaccgt
      121 gtcggcgccc cagcggatcg accacaccat cttgtcgacc tcggcggcga catcggaggc
      181 gacggcggag ttgccgatat tggcgttgat cttcacgagg aagttgcggc cgatcgccat
      241 cggctcgctt tcggggtggt tgatgttgtt ggggatgatg gcgcggcccc tcgccacctc
      301 gtctcggacg aattcggggg tgacgtaatc ggggatcgaa gccccgaaat cctggccgtc
      361 gcggatctgc gccgcgagct gctcgcggcc caggttttcg cggacggcga catattccat
      421 ttccggagtg acgatgcctt tcctcgcata gtgcatttgc gacacgttgg cgccggggcg
      481 ggcgcggagc acggtcttac ggacgttggg gaacggggcg acgcccccgg agcggtcggg
      541 gccgagctgg ccattgtctt cgggcttcac ttcgcgctgg ggtacttcct cgacgtcgcc
      601 gcgggcgcgg atccatgtcg accggagttc ggggaggccg gccaggatgt cgatggtggc
      661 ggcggcgtcg gtataggggc cggacgggtc gtagacgcgc aagggcggct cgccgcagcc
      721 gggctcgagg tggatttcgc gcatggcgac ggcgagcggg ccgacgtgga ctttgcggct
      781 gccgcggatt gggccggtgg ttacggccag ctgcgcggcg gcccctccac catgcttcgc
      841 atggtccccc tccccgttcc ggggaggatc aagctcggtg cgggcgggga tatctgccat
      901 gtgtcgctct ccaaaacgga gggagagcgt ggatgccgct ccctccctac gccggtgtca
      961 gccggatcag gttcggcggg tcgcaagctg catcggctcg cctctcaacc atgtgctcat
     1021 ggtcccccgg ggatgagggc ttgtagcccc tcccctgccc cgccacaatc aagatcgagg
     1081 agacgcgcat ggatcgccgg cagtttgaag agacgttgaa ccaattgccg atcgggcgcg
     1141 acccggcttc ggtgcggcgg cggatggagg cgatggagcg ggtgctcgag cgggcgttcg
     1201 tcatcccggg catcaaccgg ccggtcgggc tcgattcgat cgtcgggctg gtgccggtgc
     1261 tgggcgacgt ggtcaccgcc tcgatggggg cgtggctggt gtgggaggcg cgcaacctcg
     1321 gcatgtcgaa attccagctg gtgcggctgg ccggcaatgt cggggtggat acggcgatcg
     1381 gggcgatccc tttggtcggc gacctgttcg acttcgtgtt cagatcgaac acgcgcaact
     1441 tgcgcatcat caagcgctgg ctcgacaaga accatcccga gacgatggtg atcgagggtg
     1501 aagtggtggc gcggccgtag ctccgccgtg ggtggcgaac tccggcctgc gccaatgcct
     1561 cagtcgaaga cccgatcgta ttgcgggttg tccttgccgg ccagccagtc gcgcgagtcg
     1621 cggcggaaga ggaggaggac ggagaggaag ttcagcccat attggacgac ggtgagcgct
     1681 atgtgcagcg gctggccctg gagcatggca ggaatgccga tgaggccggt caccaggctg
     1741 agcccggtca gcacgatgta gatccaggcc gcgacccggc tccgccgccg cgcgatgagc
     1801 cagagcagca ggaaatagag gccgaaggtg atcagcatca cgccggccag gaagccggtt
     1861 ccgagcccca cctgccggat ggccgcggcg gagttctccc aataaaggaa aatgcccagg
     1921 agaccgagca cgagcgaggc ggcgacgacg cgttcgaaca ggatgatcga tttggggcgc
     1981 atgtcagaat ccttggcgga aaagcgggag cgtcagcgga gcggggccgg cacgggcgga
     2041 gagcaaccgc tcagccgccg agctcctttt cgaggaaggt ggtgagcagg gcggcgcgga
     2101 cggcgtcctc ggcatggtcg gcgccgaccg aatggccgcc ctccagaaat tcgtgatagt
     2161 agaagggatt gccatattcg ccgaggcggg cggccatctt gcgggcatgg ccgggatgga
     2221 cgcggtcgtc cttggtcgag agatagaaga agaccggcgg gtattttacg cccggcttga
     2281 cgttttgata gggcgaatag cgggacagga atgcccaatc cgcgggtttg tcggggtcgc
     2341 catattcgtc catccacgag gcgccggcga gcaattggga atagcgcttc atgtcgtgga
     2401 gcggcgagcc ggaaatgacc gcgccgtaga ggtctgggcg ctgggtcaag gcggcgccga
     2461 ccaggacgcc gccgttggag cggccggaaa tggcgatctt gcccttttgg ctgacgccgg
     2521 tgcgaaccag gtcctcggcg acggcgtgga gatcgtcgaa gctcttctgg cgattctcgc
     2581 gaagcaccga ttcatgccag gcggggccat attcgccgcc gccgcggatg ttggcgagga
     2641 cataggcgtt gccgcgctca gcccagaaca ggccgagcgg accggcccgg taaggctggc
     2701 cggtgaggta ggtcggcgtc tgggcggcgc ggaagccgcc gtaggcgtgg atcagcgccg
     2761 gcaccgaagc cttgccgccg cttttccggc ggaccaggaa ataggggatg cgggtgccgt
     2821 ccttcgaggt ggcgaagcgc tgctcgaccg cgaacttcgt ggcgtcgaag cgggccggca
     2881 ggctctgcgc cagcacgggg gtcgcggccg ggcggaccga atagagggcg ggcggggtca
     2941 gcataccctc gaccgtggcg aaggcgaggt cctgcttgcc ggcgacgccg accagatgga
     3001 cactgctgtt ggcggcgagc ggcatcgccc ggctcgtcca gcggccgtcg gcgccgcggg
     3061 tgagcgcgaa caatttgccg gagacgtcgt cgagcgcctt gatccagacg atggtgtcgg
     3121 aggcggcgac ctcctcgatc gcctggctgc ggctcggcgc catcaccagc tcgggcgcgg
     3181 gcgcgcggcc ggcggcgatg tcggcgagcg aataagcgac gaccgcgccg gcgggaatgt
     3241 cgcccagcgg ccggttgagc ttggcgatca ggcggccgcc gatcacgtcc tcatattcgg
     3301 cggtttcggg cagcggcgtc ctgaccagct tgccgtcctg ggtgagcagc gagaggtcgc
     3361 tggtccagaa ggtcttggcg cggttcagca tcacccagcg cgtgtcgcgg tcgacggcgg
     3421 cgatcggcga gacctgcaca tcctccttgg cgccggtgaa gattgtcgcg gcggaggcga
     3481 gcggggttcc gcgccgccag atcttggcga tgcgcccata gcccgaggtg gtgagcgatt
     3541 ccgggccgta atcggtgatg acgagcagcc ggtcgcggtc gacccaggcg acgttggtct
     3601 tggcctcggg aagggtgaag ccgccctcga cgaacttgcc ggccttgagg tcgaactcgc
     3661 ggaccacgtc ggcgtcggtg ccgccggggc tcaatgagac gaggcagcgc gcatgttcgg
     3721 gcgcgaggca atcggcgccg tgccagaccc agctcttgcc ctccgccttg ccgagcgcat
     3781 cgacgtcgat cagcgtccgc cattgcggct tgccggcggc ataggcggcg agcggcgaga
     3841 tccgccacag gccgcgcgga ttgtcggcgt cgcgccagag gttggtgaca tggtcgccga
     3901 tcaccgcctc cggcgcggcg atctggcgct cgtcgtcgag tatctcgcgg gcgcggcggc
     3961 gatagtcctc gaagcgcggc gcccgggtga gctggtcctc cgtcgccttg ttccacgcct
     4021 tgacctgggt cagcgcgcgc tcgccctcaa tctcctcgag ccacagatgt gggtcttcgg
     4081 gggatgcggc gggcggggcg gcggcggtga ggagcaaggc ggcggcggga attgcaaggc
     4141 gcaacaaggg cgaatctccg gtggtggcag ccgcacgcta tgcggcggcg tcgggcctgt
     4201 caccagcggg ttgtcgacag gcgcgggaat tatggctata tggatggcca taaaagaggg
     4261 agcgtgaatg gcgcattaca gcgtagcggc ggcgaaggac gcgttgtcga gcctgatcgc
     4321 caaggccgaa gccggcgagg aagtgatcat cacccgccat ggcaagccgg cggtggagct
     4381 gcgcccagcg caatcggaca gccgcgatcg caagagggcc atggaaaagc tgcgagcaca
     4441 tcgtcaaaac atggcgccgc tgacgacacc aatcccttat catcgcttca gcgagtggct
     4501 ctacgaagac gaagagagtt gagcttctat ctcgacaaca gcgtactggt gccgctgttc
     4561 gtagcggagg cccgaagcga agcgattcag cattggctgg aagagtgcga gcgaccggtt
     4621 catgtttcgg acctcgcaat cagtgaattc aacgcgacca tgtccgggct ggttcggacc
     4681 aaggattact ccagagagca agccagagcg caggctgccg cattcgaacg gtggcgcctc
     4741 gagttcgcag agccaaccga gaacctgccc gtggatgtac ggctggcgtc gaagttggtc
     4801 gccgaaccat tcccaaagct gcttacgccc ggcgcgatcc atctcgcgac atgcaagcgg
     4861 atggagctga ccctggtgag cgatgacaaa cgcctgatcg aaatcgcagg gcgcctatcc
     4921 gtagacgtca taagcccacc cagcattaga gttgaagaag agcctcaacc aaggctcaag
     4981 ccgccgcgat gattccctgc caccgttttt tgcggccatg cgatggaccg tcatacgggg
     5041 cgaaagctgg catctcactt tccttgccgg gcggggcagg aagaagagag attccagctt
     5101 gcgctggaat gacggggtgg cggcggcgcg gacgacttgc ccgtagacgc ttctgagctt
     5161 gtcccgaatt gcgaggaacc gtcatgccag cataagctgg catccccctt tcctctccgg
     5221 gggaagaagg aagggagatt ccagcttgcg ctggaatgac ggtattccgc accggggagc
     5281 gaaccccctc gaggcgctgg cgcagcactg gcccggaaga cgcccctaga aaatgaaatc
     5341 cgtgctttgg agcgggatct cgccggtgag ttcgatctgg aagtcggcga ggccgttgcc
     5401 gtcggtgtcg ccttcgatga cggtgatgcc gcccgccgcc gtatagcgca gctggccggc
     5461 caccgagccg aaggcggcgg tgccgatgaa ggtgaaatcc tcggtcgccg gagtggtcga
     5521 atcggcgtcg agatcgctga ggtcgatgcg gtcggcgccc tggacgaagt cgctgatttt
     5581 gtcgcgggcg gcgcccggcc cgctctcggc ctgatcgata tagacgaata tgtcggcccc
     5641 gccgccgccg gtcagcgagt ccgtccccat ccccccggtc agcacgtcgt tgccgttgcc
     5701 tccgttcaga acgtcgttgc cggcgccgcc gttgagattg tcggtggcgt tgccgccggc
     5761 gacattgtcg ttgccgctgc caagcgtgaa gatgaagcgc tcgatcgagg tgaaggcgat
     5821 gctccggtcg acgaagttgc tgaacttgcc gtccacgccg ccgcccgggt tggaggagta
     5881 attgacttcg atctgaagct tgtgggccga gctgccatag tcgatcacga gcgtgtcgaa
     5941 gccgctgccg ccatggacgg tgtcgaggcc atggcggacg gtggcgacat cgttgccggc
     6001 gccgagcgag atcagttcgt tgcgggcgaa gtcggtgccg tcgaagcggt cggcgaaacc
     6061 cgacccgacg acgttctgga aatattcgaa gctggtgaag gtaccgtagg cgccgctgtt
     6121 gacgccgcgg gtgaggttga tgaccacgcc ccggtcgtcg ccggagaaat ccgccgagag
     6181 tccgtcgacc ccggtgccgc cattggcctg gtcgacgccg ccggccacgt cgacatggtc
     6241 gtcgccgatg ccggtgaaga cgagatcgtt gccggagccg gtggagaaat tgtcggcgcc
     6301 gctgccgcta tagatttcga aatgctcgat gccggtgaag ttcacgctgc ggtcgacgaa
     6361 gttgctgaac ttgccgctct ggccgtccac tccttcggac ttggctgtct cgtcgcgcag
     6421 ggcatggccc gaggcgcgat agtcgatcat gagcgtgtcg gagccggtcc cgccgttgac
     6481 cgtatcgatg ccatggaaga ccccggcata gtccgcgccg gcggcgagat gaaagatgtc
     6541 ggtgcgggcg gcattggtgc cgatgagccg gtcgtcgtgc gacgagccgg tgacggtgcc
     6601 gtaatgctcg aacgagcgga acgcgccgta gccgccgcta ttttcgagat tgcgaaggtc
     6661 gatcaccacg cccgcggcct gcaccgacca atcggccgat agcccgtcgg tggcggcgcc
     6721 gccgtcggcg gtgtcgaagc cggagccgac atcgatgaga tcgtcgccgc cgccgctcgc
     6781 cacgacgtcg tcgccggccg cggtggcgaa gatgtcggcg ccgtcgccgg tcttgatgtc
     6841 gaaccgctcg atgccggtga agctgacgct gcgatcgacg aagttgctga acttgccggt
     6901 gaacccgctg ccgtcgctcg ccgtgatggc gccctcgttg cgcagaccgt ggccggagct
     6961 gcggtaatcg atggcgagag tgtcgaggcc gccctggcca tggacctggt cgtggccgtt
     7021 gacgacgccg gcgatgtcgt cgccgccgcc gaggttgaag atgtcggtgc ggctggcggt
     7081 ggtgccgatc agcgtgtcgg cgtgcgagga tccgttgacc gtcccgaaat gctcgaacga
     7141 ggtgaacgag ccgtaggcgc cgctgttcac cgcgaagcga agctcgatca cgacgccctc
     7201 ggtctgcccc gaccagtcgg ccgacaggcc gtcgacgtcg gcgccgccgt gggcgatatc
     7261 cttgcccgag ccgacgttga tgaggtcgtc gccggcgccg ctggcgatga cgtcgttgcc
     7321 ggtgccggtg cggaagatgt cggcgccgct gccggaggtg atgacgaagc gctcgatgcc
     7381 gctgaaggcg acggtgcggt cgacgaaatt gctgaacttg ccgctgtggc cgctgaccgc
     7441 atcaccggtc agctcggtat cgaccctgag ggcatggccc gacgcgctgt aatcgagcat
     7501 cagcgtgtcc cggcccgagc cgccggcgac attgtcgtgg ccgtggaaaa cgccggcgag
     7561 gtcgttgccg gcaccgagca ggaaagcgtc gttgctggcc gagccggtgc cgatcagcga
     7621 gtcggcaaag cgggagccga tgacggtgcc gaaaaattcg aaccgggaga aggagccgaa
     7681 cacgccctcg ttggtctcgt tgcggaggtc gatgacgacg cgcgcggcat cggcggaaaa
     7741 gtcggcggac aggccgtcga tcccgtcgcc gccgtcggcc gtgtcgacgc cggcgccgac
     7801 attgacatgg tcgttgcccc cgccggagga cacgacgtcg tccccgcccg ccgtcgcgaa
     7861 gtcgtcggcg ccggagccgg tgacgatgtc gaagcggtcg atgccggtga agctcacgga
     7921 acggtcgttg aaattgctga atttgccgga aaagccgtcc gctccggcgg tggaggcgag
     7981 ctcgttcctg aacgcgtgcg gggatgcgct gtggtcgatg gcgagggtgt cgattccggc
     8041 gccgccatgg acggcgtcat ggccgtggaa gacgcgggcg gtgtcgttgc cgccgccgag
     8101 gttgaaggtc tcgttgcggg cgacggcgct gccggtcagc acgtcgtcat gcgcggagcc
     8161 gaagacggtc ccgaaatatt cgaagctgcg gaagctgcca tagccgccgc tgttgaggtc
     8221 gttccggagg tcgacgacca cgccggccgc ctgctgcgac caatcggcgg agaggccgtc
     8281 ggtgccgccg ccgccattgg cgacgtcctt gccgcggccg acattgacat ggtcgtcgcc
     8341 gaggcccgtc gcgacattgt cgttgccgcc gccggtggtg aaatcgtcgg cgccggcgcc
     8401 gctcatgatc gagaagcgct cgatgccggt gaagtttacg ctgcggtcga cgaaattggt
     8461 gaagccgccg tcgaagccgc cggcgggatt ggcggtgacg gcgccgttgt tgcgcagcgc
     8521 atgggccgaa tcgcgccagt cgatctcgag catgtcgaag cccgacccgc cggccgcggc
     8581 atcggtgccg gcgccggcga tgaacaagcg atcgttgccg atcccgccct ggaggtcgtc
     8641 attgccgagg ccgccgtcga gataatcgtt gccgctgccg ccgatcagct ggtcgttgcc
     8701 ctcctcgccg gagatgcggt cgtcgccgcc gagcccgtcg ataatgtcgg ggccgctcgt
     8761 gcccaccagg atatcgtcgc cattcgtcgg aaccgccatc gcgccctccc cctgtcgcgc
     8821 gcgcccatgc cgcagggtgg gcgcgcccgc gccgccaccc cagccctgtc gcgccgtcgg
     8881 gattggtgat aaaggtccaa cgcgcccgcg agtatccgcc tgaaagggac agaacggggc
     8941 atctcgggac atccgcgtgg agagcaacgg caaaagcacg gcaaaagaac ggcttagaag
     9001 ctggaaggag attgccggct tcttcggcac cgacgagcgc accgtcaagc gctgggcggc
     9061 cacgcgcggc ctgcccgtgc agcgcgttcc gggcgggacc cggaccccga tctacgccga
     9121 tgtcgcggcg ctgcagaatt ggctggccgc ctcgcacgtg ggcgcggccg agacgatcgt
     9181 cccgcccgcc gagccgccgc cgcccgccgc gccggcccgt cgcgcccgct cggcgccgct
     9241 ggcggcgctg gccggagccg cggtggcggc ggcgatcgcg ctggcgcttt acctgggccc
     9301 gtttcgtgac ggcgaggcga gcgcccacca gccgccgcag gaggcggtcg agctttacct
     9361 tgccgggctg ctccattggg agaagcgctc gcccgagagc ctggcccggg cgacgcgcta
     9421 tttctcgcag gcgctggcgc gcgaccccga atatgccgag gcctatgccg ggctcgccaa
     9481 ttgctacctg ctgatgcgcg aatataccgg catgccggac gaccaggcct atccgatggc
     9541 ggaggcggcg gcgcggcggg cactgagcct cgaccccggc ctgtcgcagg cccatgccgc
     9601 gctcgccttc acgaccttct actgggcgcg ggatttcgag cgggcgcagc aaagcttcaa
     9661 gcgcgcggtg gcgctggatc ccgcttcggc gcatgcccgc cattggtatg cgacggcttt
     9721 gctccatgtc ggtgacatga aaggcgcgct cgagcagatc aacgccgccc agcggctggc
     9781 gccgcaatcg gcctcgatcc tggccgacaa gggcctggtg ctgttccacg ccggccgcgc
     9841 gcccgaggcg atggcgatgc tgagggaggt ttcggcgctc gagcccgaat tccagtcgcc
     9901 gcacgtctat ctttcgatca tccagctcgc cggcggcgag gatgcggagt tcctgcgcca
     9961 cgcccgccac gcggcgcggc tgcgcggcaa cgaagcgcgg ctggcgctgc tcgacgacgc
    10021 ccagcgcggg ttcgaacaag gcgggcggcg gggcatgctg gaggcaatgc tggcggcgca
    10081 gatgcggctc cacgccgagg ggcgcgaaag cgcctatgcg gtcgcggcca cgcaggcgct
    10141 gctcggcgac cgcgcgcagg cgctgcgcta cctccggaaa tcggtcgagg agcgggagcc
    10201 gctcgcggtg ggcttgcgga tcgagccggc cttcaagggc ctgcgcggcg atgccggcta
    10261 ccaggcgctg gccgggcggg taggaaaccc ggcggcgaaa tcgcggcggg tggtcgcggg
    10321 ttaaaccgcg ttgaagaacg tcgccccggt tccgatcggg ctgagcctgt cgaagccctt
    10381 ctttttggtg tcagaacaag aaggaaggcc cttcgacagg ctcagggcga acggaggtgg
    10441 gatttccatc ctagtcgttg aggctcccga tcaggcgcgg gcgaagaccg gcgggcggga
    10501 tagccggcgg cgaaattggg gcgggtggtg gcgggttaaa ctgcgtagtc gaagtacaag
    10561 tcgccccggt ttccgttcgg gctgagcctg tcgaagcccg ttcctgtttt tctttttccc
    10621 ttcagaacaa ggaacaagaa ggaaggcccc cttcgacgcc gctcaggaca ggcttcgaca
    10681 ggctcagggt gaacgggtca ggcgagttcc tctctgatcc tccggcgctc ccggaatcag
    10741 gcgcgggcga agacggggcg gccgacatcg ccctggccta ccgtccgccc ggccatgtcg
    10801 agcatgcggt cgagcgactt cttggcggcg aggcggagat cctcgtccat ctcgatgcgc
    10861 ggctggagat cgcgcaaggc gacgtagagc ttctccatgt tgttgagcgc catgtacggg
    10921 cacatgttgc agttgcaatt gccgtccgcg ccgggcgcgc cgatgaaggt cttgtgcggc
    10981 gccgctttct ccatctggtg gatgatgtgc ggctcggtgg cgacgatcat cacctccgaa
    11041 ggcgagctca gcgcatattc gaggatcgcc ttggtcgaac cgacatggtc ggcatgatcg
    11101 aggatgtgcg gcgggcattc gggatgggcg aggaccgggg ccccgggatg ctgcgccttg
    11161 agcttcaaca gctcggtctc cgaaaaggcc tcgtggacga tgcagacgcc gggccagagc
    11221 agcatgtcgc ggccggtctt gcggacgaga tagccgccga gattcttgtc cgggccgaag
    11281 atgatcttct ggtccttcgg gatctggtcg aggatggtct cggccgagga ggaggtgacg
    11341 atgatgtcgg acagcgcctt caccgccgcc gagcagttga tgtaggtgag cgcgatatga
    11401 tcgggatggg cttcgcggaa cgccttgaac tggtcgggcg ggcacgaatc ctcgaggctg
    11461 cagccggcat tcatgtcggg cagcacgacg atcttctcag ggctgaggat cttggcggtc
    11521 tcggccatga agcggacgcc gcaaaaggcg atcacttcgg cgtcggtcga ggcggccttg
    11581 cgggacagat cgaggctatc gccgacgaag tcagcaatgt cctggagcgc cggctcctga
    11641 taataatggg cgaggatgac ggcgttgcgc tccttgcgca gccggtcgat ctcggccgcg
    11701 atgtcgaggc ccctgaggct aagattcggt gcgttcatct cttgtccccg tgcggcagtg
    11761 cctagcggct cccctgccgt tccttcacta catcttccat gcggcgcgac ctgtcgagcc
    11821 gggacgggtc ctcacccgcc tcgtcggcaa accgcaccac cacctgggca tccagccccg
    11881 ccgcgcgaag cggcatcgcg aagctccgct caacggcgcg ccgcgccgca tcgcgggcca
    11941 gccgcatcgg cagcggctcg cgcgcctggc ggaccaattc ctgccggccg cgctgccggt
    12001 tggcggagtc gagcgccttt tcggcatcgg tgagcgccat caggacgccg ccctgatcat
    12061 attcgcgaac ctgtgaaaga tcgatatcgg ggccggaaat ttcaagtagc ggcaaggaga
    12121 tagtgagcgt cttggagggc tcgtcccagg ccagatcgcg ctgctggagc ttggcaagat
    12181 cgacctcgta gcggacattg cccggcatga tcaaagtctt ctgcgcggtg aggccgaagc
    12241 gggtctggcg cgaggtgacg acggcgacat agcgcgcggc gaacggggtc agccgcgcct
    12301 gctcgcgcat cgattgcagg ctggcgacgg cgatcgtctc gggatcgggg ccgccgccga
    12361 acaggccgcc gagccggacc gcccccatca gcaaggcgcc gagcgcaagg ccgaggatga
    12421 gcgcggcgag cgcgagccag gatctcctca cgcggcgcgc tcccaggctt cgccgcggcg
    12481 ggcgacgcgg ccgcgcgccg cgaggtcgat gagatgggcc agcaccgacc gctcggcggc
    12541 gggatgcagg cgcgggtcga tgtcggcata catagcggcg accatcgcgg ggaccgtccc
    12601 ctgcccttcc ccgagccgcg ccaggatctg cgcctcgcgg tggcggcgat ggaggatgag
    12661 gccgcggaca tgtttggccg gctgctcgat ggcggggccg tggccaggat aatagacaga
    12721 atcatggcgg gcgaggagcc ggtcgaggct ggcgacatag gcgcccatgt cgccatcggg
    12781 cggcgagacg atgctggtcg accagcccat cacatgatcg ccgctgaaca gggcgccggc
    12841 ttcgggcagc gcgaagcaga gatggttgga ggtgtggccg ggggtagcga cggcggccag
    12901 cgtccagccg ggccctgcca gcgtgtcgcc gtcggccagc gtccggcccg gcgcataatc
    12961 ggcgtcgaag gcttcgtcgg cgccgaagcc gtcatcgggc ggtgcgagcg cggcgcagcc
    13021 gacgatgctg gcgccggtcg cggcctggag cgggcggctg gcggggctgt ggtcgcgatg
    13081 ggtgtgggtg cagacgatgg cggcgatgcg ctcgcccgcg gtggcggcga gcagcgcgtc
    13141 gacatggccg gcgatggcgg ggccgggatc gatcaccgcc acctcgcctg tccccacgat
    13201 gtagctcatc gtgcccgtga aggtgaacgg cgacgggttg ggcgccagca cgcggcgcac
    13261 cagcgggtga aggcgttcga tggtgccggg ggcgtccatc tcggccatgt ggcattggga
    13321 gaccgaaaaa ccaagcggcg gcggagggcg cccgccgcgg cggcggtcag ttgatttcca
    13381 gctcggcgtg gagctcgccc atttcgcggt cgatctcgat gtcggcgcgg cggacctcgg
    13441 cacgggcccg ctgcatctcg gcctgcagcg ccttcagctc gcgcgggttt agaccggccg
    13501 gggacagcgg tccgcgaaca tggagccggg ccgggccgtg agcccgctgg agcgacgcgc
    13561 gggccagctt caccccggcg cgggcatgga caaggccttg ggcgcgggcc tccgcggcgg
    13621 cttcggcgcg ggcctcggca gcctcccggg cggcctcggc ggcgtcgcgc tgagcctcgg
    13681 cgcgggcctc ggcggcttcc cgcagcgctt cggcacggtc gcgaagcgct tcggcgcggt
    13741 cgcgggcggc ctcggcgcgc gcttcggcgg cttcgcgcag ggcttcgccg cgttcgcgga
    13801 gggcctcgcc gcgttcgcgc agggcttcgg cgcgggcgtg cgccgcctcg ccccgggcat
    13861 gagcagcctc gcgcagggct tcggcgcggc cgtgcgcggc ttcggcgcgg gcgtgggcgg
    13921 cttcgctgcg ggcatgggcg gcctcgcgca aggcatgggc ccgctcgcgg gcggcgtggg
    13981 cgtcgtcgaa ctcgtcgtca tagatgcggt cgaacggctc caccgccagg gccgcgtaac
    14041 gcacggcggt ctcggcgacg cggtccgcca ggcccggctc gggccgggcg gcggcgagct
    14101 ccacccgggc gcgatcgatc gagcgggcga cgatcgcctg gacttgcgga tcgcttgccg
    14161 cggccggatc ggagcagagg atgacccgcc cgacggcggc gccggagcga tagcgcggcg
    14221 ggttctcata gccgccgggc gcgcatttgg cggcgagcac ggccctgtcg cgggcgccga
    14281 cgacgggcgc gatgtcgccg ccgcggctga ccagggtctg gggcgcggcg gcgccatcgt
    14341 cctgctgggc gaagccgggg ctgctgaggg cgaggccggc gacgccgagg atggcgactg
    14401 agacgagccc ggcgaggctg cgcaggcggc tcgagcgatg ctgcttgacc atcatcagcc
    14461 ggcgcttgag ctggtccgcg cggttcatcg ggcaggcggc gacctcgccg gggcggctgg
    14521 cggatttgac catcgcgaag gcgtaatcga gccgccggtc cggggcgatg cgggcggtga
    14581 cggcggcgtc gcacgccagc tcctgatcgg tgcggaaggc gcggaaggcg atccaggaaa
    14641 ccgggctgaa ccaggtcagc gccagcaagg cgagcgcgac gtaattccag aaaatgtcgc
    14701 cgcggcgatg gtggaccagc tcatggtcga gcgccaggcc gcgctcggcg gcgctgtagc
    14761 gggcggcgaa atcgagcggc acgacgatgc gcttttcgag gaggccgacc gcgagcgggc
    14821 cgtcgaccgc gccgctttcg acgaccttga cgccgccatg gatcggcggg tcgcccggcc
    14881 ggcaattggt gccgaggctg agcaggaacg cgctataggc cgattgctgc cacatcacga
    14941 aggcgccggc gccgccggcc cataaagcga gcagcaaggg cagccagtcg gcgaggccgg
    15001 cggcggccgg cgcggcggcg gccgcctcgc ccgcggcggc ggcggcgggg agcattatca
    15061 ccgtatattt ggggagcgcc tcggccatcg cgccgccgag cagctcgacc ggcggcatga
    15121 acaggcggag gagcggcagc agccacaggg cataggccca gccggcgccg aacgcccgcg
    15181 ccaccggccg ccgcaccgcc agcacgagca ggagcagcaa ggtgacgccg atcagcgtgt
    15241 cgagcgccca ggcggtcacg gcttcagcgc cttcagaagg gcctcgatct cggcaatgtc
    15301 ctctgccgag aggcggtcgc gctcggcgag ctgggcgacc aaaggcgtca accggccgcc
    15361 gaacatgcgg tcgagcaatt tgcccgattc ctgcgcgaca taatcttcgc ggcggacggc
    15421 ggggcggtag aggtagcggc ggccgtcctc ctcatgggcg agcgcgccct tggcgagcag
    15481 gcgggagagc atggtcttga cggtgcggac gttccagtcg cgctcggccg gcacgcgctc
    15541 ggccacttcg gcggcggtga gcggcgcctc gtcccagagc acctccatca ccgcgaactc
    15601 ggcgtctccg atccgttcca ccttcgcgcc tccctataga ctacaggagt agtccgaatt
    15661 tgcctgaacg gcaagtgaat gtcggcggtc agtggctttc gcgggaccgg cgcgcgccgt
    15721 cgagggctgc ttcgaggtcg cggccgatcg cttcgaagtc gatcgcgtcg atctcagcgt
    15781 ggacccgccc gagctcggcc cggagccggg cgcgctcggc cgggccgaaa tgggcggcgg
    15841 cgagcccggc gtggcgctcg gcgacggcgg cggccgcaac cgcgcgggcc accgcgccgt
    15901 ccaccttggc cagttcagca tcggtttgac gcagcgccgc gcgcacccgc gcatcgactt
    15961 cgccgttcaa ggcggccttc acttcggggc ggcgcatcac gcggtcgaga tgggccttga
    16021 cccggctgtc gatgcgcgcg gccagcgcgc gaacttcggg gtcgtcgacg cggcgcccgt
    16081 tgctgcagac gagcgcgccg ccgttgaact ggatgccgga gtttcgggcc ggcccggtgc
    16141 cgcatttggc gcggatcatc gcctcgtccg tctggctgag gatcggccct tcctcgtcca
    16201 acgcgcgctc gatcgccgcg gcggccgcgc cgggcctcgc gtcggcggct ccgaagccgg
    16261 cggccgaggc ggcgacagca gcgcaccgag aacgaggatc ccgccaaagg tgcgacgacg
    16321 gactttgccc aggatcatgc cggcctcctg ctgtgtgtga ttacatatgt agtcatcagc
    16381 gggctgaacg ccggcttaac cggacgcgcg ggcgagcgtc ggcagggctt acagacggag
    16441 caaaggttaa actcgcttca ccatcggaag ccgcggaaaa gcggttgcgc gccagactgg
    16501 cacggagcgt gtatccttat ccgcgtccac attgttccac gaaaggggcg gactggcgct
    16561 ttcctggtcg cgccgtccgc cccagtcatt tccggaaaat cccgcgccca ggcgccgagg
    16621 ccaagtttgc ggcacggggc gcatgcttgg ggtttgccgt tcttgtgccc ccgcgaaggc
    16681 aggggcccag accgggcgaa agcccgccaa ccgatctcgc ctagacatcg cgccagtcca
    16741 tattgcgcca agcttccgaa gccgctgcga tgaccgggct accgctgacg cggcaacccg
    16801 gggctgggcc cctgccttcg caggggcaca atgctctcgc ttccgatgcc gtcgctggcg
    16861 agacgatcag gccttggcga cctgcttctc gtcgaccagc tgctgaagct cgccggcctc
    16921 gaacatttcc atcatgatgt cggagccgcc gacgaactcg cccttgacgt agagctgggg
    16981 gatggtcggc cagtcggaat aggccttgat gccctgacgg acctcctggt cctggagcac
    17041 gtcgaccgtc tcgaacgaga cgccgagatg ttcgaggatc gagacggcgc ggctcgaaaa
    17101 gccgcattgc gggaagagcg ccgtgccctt catgaacagg acgacgtcgt tggatttgac
    17161 gatctcgtcg atgcgggcgt tgttgtcgga cattcttaag gctcctattc gggggtcgcg
    17221 gtggtgagct ggagcgcgtg cagctccccg cccattcgcc cgcccagcgc ctggtagacg
    17281 cgctgatgct gcttcacttt cggcaccccc cggaaacttt ccgccaccac gcgggcggca
    17341 taatggtcgc cgtcgccggc aaggtcggtg atctcgacgc gggcatcggg gatgccctcg
    17401 cggatcagcg cctcgatgcg gtcggccgcc atcggcatta gctttgggcc tcgatcatct
    17461 ggcggcgcgc ttcgacggtc ttgtgctcga gcgcctcgcg gatgcgggcc tcgtcgatct
    17521 cgacgccggc cgaggtgagg tcgcccagca ccttgcggat cacgtcctcg tcgcccgctt
    17581 cctcgaaatc ggcgcggacc acgtccttgg cgtaggaatc ggcttcgacg tcggtgaggc
    17641 ccatcagatt ggcggcccag gcgccgagca ggcgattgcg gcgggcgatc acccggaact
    17701 gcatctcctg atcgcgggca tacatgctct caaatgcctt ttcgcggtcg tcgaacgtgg
    17761 tcatggcggt ccttcggctg ggatgtcgct ggaacagata ggcgggcggg ctgagcggcg
    17821 caatgtcgca gcgtcagtcc acggtcacca cgagcttgcc gaccgcccgg cgcgcggcca
    17881 tgtgggcgat tgcctcgccg gcttcggcga gcggccaggt gcggctgatc cgcggcgcga
    17941 tgctgccctc ctcccacagc cggaacaggg tgcggatgtg ggcggcattg gctttggggt
    18001 cgcgcgcggc gaaggccccc cagaagacgc cgcagacgtc gcagctcttc aagagggtga
    18061 ggttgagggg caggcgcggg atgccggccg ggaagccgac cacgagatag cggccctccc
    18121 aggcgatggc gcgcagcgcc ggctcggcat agtcgccgcc gaccggatcg tagatcacgt
    18181 ccgcgccatt ggcgccgacc gcgtccttga attgctgggc gagcgccttc gactggtcct
    18241 tgtcgaacgg cccgcgcgga tagacggtga ccgcgtcggc gccggccgcg cgcgcggcct
    18301 cggctttctc ctccgacgac accgcggcga cgacgcgcgc gccataggcc ttgccgagct
    18361 cgaccgcggc gaggccgacg ccgccggccg cgcccagcac gagcagggtc tgcccctcct
    18421 gaagacggcc gcggtcgagc aaggcgtgga tcgaggtcgc gtaggtgagg atcagcgccg
    18481 atccctcggc gaagctgcgc cccgccggca gcggcagcgc ctggcgggcg tcgaccagca
    18541 ccttctcggc cagcccgcca tggccgagca tcgcgatcag ccggtcgcct tcggcccagc
    18601 cttcgacgcc ctcgcccgcc gcctcgacga tgccggcaat ctcgccgccc ggcgcgaacg
    18661 gccgcggcgg cttgaactga tatttgtcct cgatgatgag gacgtcggga tagttgatcg
    18721 cgcaggcgcg gacgcggacc agcagctggc ccttgctggg caccggatcg ggaagctcgg
    18781 ccagccgcag cgtctccggc ccgcccggct cgttcgacat caacgccttc atcttcgctc
    18841 cccttccgct tgtcggcccc gccacggcat tctcgttcgc ccggtgcgaa tcaccggggt
    18901 gctcagctac gccaggccta gggtgcatgg gaaacgacaa gcgcggcttt cagggacggg
    18961 tcgaacgtcc gcaacgggtg gaaaggcgac attcccggtc ctacgatccg cgggcatggc
    19021 ttacctcccg atctggccgc tcctcctcgc cgttaccggc gcaaacggtg atccgccttg
    19081 cgaggcgcga tcggcggcgc cggtgaccat cgccgaactg gccgcggagc cgcgccgcta
    19141 caaggggcgc tgcgtggcgg tcacggcgcc cgccacgcat tggtccctgt tcagcagcac
    19201 gccctcgctg gagcggcagg tgcgatcatc caggtccgcc cttgtccgcc gatcggaacg
    19261 gcgtcgccag gtcggctggt atcacccgcc gggcgagctg gaccctcaag tggagctcgg
    19321 cctcgcgaaa ggcatattgc tcagcgtcag gggtatcgtg gacgattgcc acacgcttcg
    19381 cgagcgggag ctgagcgagg ccaaggccga gtaccggcgg gagctggccg cgcggccgcg
    19441 tgagccggcc cccccacctt ccgacgagga caggcttgtc gaggaaggcg aaccctccgc
    19501 ggtcttcttg ttcggttatt gccactggaa tccgggcacg atcatccggg ccgtcgccgt
    19561 aacccctgcg accggacggt aagatcgagc gcggcgcggc tccgaatgcc cgcgatggat
    19621 tggaaagccg gcatggctta tggcctgatc atgaaagccg ctgccctgcc ggccgtgacc
    19681 ttgccccagc taagctcggc gttggaggcg agctgggatc gccgcaccgc ctatctcgga
    19741 gcctgccggc cggccaaccc ggccttgggc caatgctatc ccacctcccg cgtggtgcag
    19801 tggttcttcc cccggctgga gattgcgtcc ggggaagtgg atacggggtc ggggttcgag
    19861 gcccatttct ggaacgtgga tccggcgtcc aacccggcca ggcacgtgga cctcacctgg
    19921 cggcaattcg caccaggctc gaaggtcgcg cgcttcgaga tcctcgatcg cgacgcgctc
    19981 aacgacactc cgccgaccat cgcgcgctgc gagctcctcc tgagccgcgt tctcctgaag
    20041 ctcgaagcgg ccggtgccat cgatcggaca ggcggagccg catcaatgcc tgccttgggt
    20101 ggacatccgc cgaacgtccg ggatggatag gaagcggacg ctcggaatgc taccgatcgg
    20161 tcatgtccga taaatatgag ccgccgtccg aattcttgaa gaccctcatc gaagatgatt
    20221 catcgctgag aggggacgaa cggtcggacg cgaacatacg ccgcctgatc gaactgatgc
    20281 gggacaagga tccggccaac cgggattggg cgacgtctct gctcgccggg acggacgccg
    20341 acacgcccga agtgcgcggc gcgctcgcgg cagccgctga agacgaacat gcgcagactc
    20401 gttctgaagc gatcctcgga atcgcgcaac gcaacccgga cgctgcgtta ccgctgctcc
    20461 agcgtgagct tgccggtgac atggtcacca tggccacgct ggaagcggcg atgctggtcg
    20521 ctcacccctt gctggttgcc ggcttgcggc gcttcgcctc accatcggac cacgccccga
    20581 tcgacgagtg tatcgatgag gctctgactg cgtgtgaggc gggaaagccg cccgccgact
    20641 actgggccga tagcgacccg ttcaatattg cttggagagc cgacccgtaa aacgggtccg
    20701 cgggaaacgg gcgctgtcag ccaaagccgg agaagccagc gctgtcggct gcgtacccgg
    20761 gacaaggtgc aagaatgccg tcgtcgggtg gaaagcggac gttgccgcta caggctcaaa
    20821 gcctcgcaca cgtcgatcgc atatattcca gcagcccctt tctcgaactc ggcgcgccag
    20881 taagcatagc cttggcggaa ctcgtcccca ggcgcgaccg tcgcaaggag ctgatcagcg
    20941 ctcctgagga acgctggacg agcaaagggc atgtggccga cctcaacctc tccggcgctg
    21001 tcgaggatgg acacgtgcac gatctcctca tcgcccagcg tctcgatgcc atgcacggct
    21061 gctctgtagg aacagccttt gagggaccag acttgccccg tcccgagagg agttgcagtc
    21121 aatgcgggac cttccttggc tcaaagtcgg ctaacgagcg gcagtaacag gcttccatcc
    21181 gacgatgatg cagcggcttg cgaacgtccg caatgggccc aacgctcgcg tccgtcgcgt
    21241 cgcgggcaac gccgccaccg ccatggccgc cgggatggcc gaaggtcacg agggcgcaag
    21301 gcgacttacg gcacttattc gaatgggtcc tcagcggcat agctgaattg aggccgccat
    21361 tcagtcggag cggccatgga aggagtgaag gcgatgggca atctttcgac aatcggcggt
    21421 gcgacaatct gggtggcggt cgtgttcgcc ttgatgctgg cggtgatcga gccggtcagc
    21481 gtcacctctt ccaccggcca gcccgcttcg ggagccgctc agggggagac gaaagctccg
    21541 gcttcgcaat gaggctgacg tccgcagggg ggcggccctt cgacgccttc aaatatccgc
    21601 catcgaaagc agacattcgc cggagggcaa atcggcagat ggcttggtgc cgggtccggc
    21661 tccacgatcc caagctgctc ctgcactcgc cagagtagct cctcggaacg gcagaatccc
    21721 ccattgtcag agggtggtcg gggagacagg attcgaacct gcgaccctct gctcccaaag
    21781 cagatgcgct accaggctgc gctactcccc ggcgtagcgc acacgggaac agcgaaagct
    21841 gttcccgaga cgcgcaagcc cggccggcgc gctgcgcgcg tccgacggcg cggctttgcc
    21901 gcgacggggc cgggatggca agggcccagg gcaggcaatg agtaagcggc gcggcaaagc
    21961 cgcccttcga ctgcctgcca aggcaggcgc tcagggcagg cgagcgagca aagctcgctc
    22021 gtcggtcctc gcgggacgcg aggccggccg cacgcgctgc gtagtcgatt tagcttcgct
    22081 aaatcgtgct cgaagcgcgt ggtgggcccg gagggattcg aacccccaac ctagccgtta
    22141 tgagcggccg gctctaccgt tgagctacag gccctggctc gcgccggagg cggcgatagc
    22201 ggagcgggat cgggcttggc aagctagcgg cgggccggcc gcgccccgcc ggcggcggcg
    22261 aggatttcgt cgaccgaggc gggcgcgacg ccgcggcggg cgaacaggtc ggtcaccccc
    22321 tcccaccagg cgtagaacag gtcgaggtcg tcgcgggtgc ggacgtaagg cgtgtggccg
    22381 ggctccagcg acggcgagtg gaaagagatc gagaagagct ggaggccgtc atcgagcagc
    22441 cggtcgatcg cttcgagcac ctcgccgacc ggcatcccct cgggcgtcag cgcgacccgg
    22501 ccgagcaggc gggtgcgggc gagcaggccg cgcaggtgcg gcaccctccc cgcccgcgga
    22561 tagaggttcg ggccccgccg ccgcagcgcg ccgacataag ccgaggtgag cggcacttcg
    22621 agcagggcgc cgccgcagag ccggtagggc agcgggcgga tgccgctgaa atcggggccg
    22681 cgctcggggc tgtagtcgaa cagcgcccgc accgagacgt cgagcgtgta gccggcttcg
    22741 accagcaggc cggccgtgtt cggcccgacc ccgtagcggc cagcgcggta gacggtcggc
    22801 cggcgcccga acgcctgctc gatcgtatcg gtgaggcagg cgagcttggc gcgttcgtgc
    22861 tcggccggaa ggttgccgac gaagctgttg cgggcgctca gttcctcggt gaagggcggg
    22921 ttgacccagg ggtggagctg ggtgccgacc gtgcattcgc cggcttcctg atattcgcgc
    22981 aaggtggcga cgcagcggac gtcggtggcg atcggatggt cgaccagata ggtcggcgcg
    23041 atgccgtagc cgcgcagcct cttgtggatc agcggcagcg attcggcggc ggtggtggag
    23101 cgctcatcgc ggctcggcgg ctgcgaccag tcgaactcct cctcggtgtc gacgaagacg
    23161 gcgaagcggc ggccgaacga ttcgggcagg cgcaaataag cgcggtcgtc gggcggccgg
    23221 tcgagtctgc cggagccggc ctcttcgtcg agatcgccgc gcggcaccgc cgggcttaga
    23281 cgccttggcc ggtggatcgc tcgatcgcct ggaaagcggg aaggtagagc gagaaacgct
    23341 cccgcccgat gacgagcgcg ccgccggctg cttcggcgag gttgcgcacc agccgcagcg
    23401 cgaagccgag gccgagcgcc ggcgcgccgg gccagtcgcc ttcggggctg tagccgggat
    23461 cgagcaaggc ggcttcgtcg aggccgtcga tcgcggccgg ccggtcgatc gccaggcaca
    23521 gcatctcgcg cccgccgagc ggctccagcg ccatcaatgc ggcgatggtc tcgccttcgc
    23581 cagcgaggcc gatggtggcg gcgagcatgc gcgcgaacat ccgttcgagc gcgccgagct
    23641 cggccttgac cggcggcagg ccgcggctga tctcgatcgc gagccgggcg ctgcgctggc
    23701 tggcgacccg ctcataggcg gcgtgcagcc gctccagcaa agcggcggcg tcgaccgagc
    23761 cggcgtcgag gttgagccgg ctggtctcga tccgcgcggc ggtgtcgaga tcgtcgacgg
    23821 cgctgagcag ccgggcggcc tgggccatga tctcgccggc gcgggcgcgg tagcgggcat
    23881 gggccggccc catatactgg ccgtcgatca gctcggcgaa gccgacgatg gcgttcaagg
    23941 gcgtgcgcag ctcgtggatc agctggcgca gcgaatcggc cggcaggtcg gagccgaaca
    24001 ggccggcggg catttgcggg ccgtgggcgc gggcgacctc gtcgacccgc ggccggcgcg
    24061 cggtgccgcg atagccgaga aagctgccgc tgccagcgtc gaagaaggga acgcccgaaa
    24121 tgcgccagtc gcccgcggcg gggccgtcgc cggcgacgct gaagcgggcg tcgcggaacg
    24181 gcgcccgttt ctcgaacgct cccgccgcct ggccgtcaat gccgtaataa gcggggcccg
    24241 cgatattggc gatgctctgg ccaacgagcg gcccgcgcgg cgcgtcctcg acccacagga
    24301 tgacgccgtc ggcgccggtc tcccagcgga agccctcgac cggacggacc acggcctcga
    24361 ccgggtcggg cgggggcgag gcggcgcgat gcttgcggaa cgcctcgatc ctggcgacca
    24421 ggtcgcgaat ctgcgcttcg cctttgtcga ggcccggact cagcgcgccc tcgccggccg
    24481 cgccctcccc cgacccgcgc tcgcggggcg ccggagcctc gccgatcgtg ccggacagca
    24541 cgaagtcgct ggcgccgaag ctggccagcg cctgcaccac ctgcggcggc aggtcgcggc
    24601 ggtggcggag caaagcgcgg gcggtcgggc tcagcgacgg cagcagatcg agccattcgt
    24661 cgatggcgag ccgggcgccg gcgatcagcg gcccggcgac gaccggctgg tcctcggcga
    24721 agaaacggaa caggtcggcg gtgatcgccc ggccggtcag cgacatcgcc acttccttgc
    24781 gggtcgcgcg gtcgatctcg gcccggtgct cgcgaagata ggcgaaggct tcctcgagcc
    24841 tcgctccctg ccgcgtccgg gcctgggcga ggatgtcgac caactggcgc cacttggcgg
    24901 cgcgggccga cgcgctgctc gcgggcaatg ccatcaccgt cgcgattctg tcgtcgaaac
    24961 gcacgcctcg tcccctcgcg cgaagaggcc gactctcgcg caaatacaat cgttaacgcc
    25021 gccgcggcca atggggaagc ctcgatcggg gccgcgtcgc atattgggac gagccgagcc
    25081 cccggcccta ccggacgcaa aaaaagggcg ccggcagcgg ccggcgccct tgctcgtcag
    25141 ggggttcggt tcagccgcgc tggctgagcc agagcagcca gaaagcggcc agctcggcgc
    25201 gggcgccggt caccgccttg aacgccgcct cggcctcggc acgctggccg gccagcgcca
    25261 aggccgcgcc gagccgggta ttgaccaaat tggcgtcgac cgagcctttc tgcagcgcgg
    25321 agcgatagag gccggcggcc ttggcatagt cgccatagcc gaaataggcg tcagcggtgt
    25381 tgagcgcgag cgttccggtc ccggacgcca tcgctttctg ctcgagcccg gggagcgcgg
    25441 cccggtcctc ggctatgcgt ggcccggtct gcgcgccgag cgccttgaac gagggatcgt
    25501 cggccgtata gacgcgcgcg gcgaggccct cgtcgaccac cgccttggcc tcgccgggca
    25561 gccccttctc gtggagcgca ttggcaagcc tgaagtaatt ggccttgtcc gtaagcgatt
    25621 tggtcgtccg catcaggcgc agcagatcca tctcggcggc cttgtcgagc cggccgttct
    25681 gctcatagac gagcagggcg gtccgccaat tctcgggcgt cggataagcc gtgacgagct
    25741 cgcggctgag ctggacgctc ttcggcccct gtttcccatt ataggcgaat tggagggcgc
    25801 gcttgtaagt atcctcgggc gccttctgcc cggccgcctt ggcggtggcg atctgctgct
    25861 cgaccaaagc cgctgcctgg gccgggttgt cgcgcgcctg catgctggcg agcgcggcga
    25921 ccatttcctg gtcgttcggg ttcagcttca gatactcggc gagggcgcgc gcggccttgg
    25981 cgttgtcgcc cgtttgttgc gcgaccgctg cctgctcctt gatcagggcc gcgcgatccg
    26041 ccggatcgcc gccgccgctc gcgaccatcg cgtcgagacc ctgcatctct acccgcttgt
    26101 cgttcgtgcc tttgccgatc tgatattgga actggccgac gaaatggcgc gcgtcggcgc
    26161 tcctcgcggc cgccgcggcg gcgggcaaag ccgcattcgc ggccgcccaa tcactcgccc
    26221 gcaccgcctt ttccagcggc gcgagcaccg cttgctccgc cttgctcagc ttcgcggtcc
    26281 agctggaggc ggcggcctgc ttcttctggg cgatggccgg agtggcgatc gcggcggcgc
    26341 cgacggcaag cgacgccgcc agggcgatgg ctgaaatagc tttcatgatc ttctcctgtt
    26401 caatccgaaa cggctcaggc cgctcagccg cgctgggcaa gccagagcaa ccaataggat
    26461 gcgagatggg tacgcggccc ggtgacggcc cgcagcgccg cctcggcctc ggcgcgctgg
    26521 ccggcgagcg cgagggccat gccgagccgg gtgttggcga cgctggcgtc gacggacccc
    26581 ttggcgagcg ccgcgcggta aagctcggcc gcggccgcat attcgccata gctgaaatgc
    26641 atgtcgccgg cgctgagcgc gagcgtgccg gtagcattcg ccatcgcctt ggtgcgcgag
    26701 gacgccaagg tcttgcgctc ggccgtggca cgcttgccgg aggcggcgat cagctccttg
    26761 taggtggcct tggccggatc gaccatcttg gccttgacgc cctcgtcgag caccgccttg
    26821 ctctcgcccg gcacgccgcc agtgtcgaac gactgggcga gctcgagata gtcgcgctcg
    26881 ccgccgagcg cgttggccga gcgcatcagg cgcatcaggt cgagccgcgc cgccgggtcg
    26941 ggctgggcga aatcgcgata ggtgaggagc gcgtcgcgcc aattttcggg cgagggataa
    27001 tcggcgacga ggccgcggct gaactcgatg ctcggcgccg gcatctgggc gtcgatggcg
    27061 tgcttgagcc cgcgcttgta ccagctctcc gggaccggct gcccggccgc cttgcgcagc
    27121 ttgatcgcgc ggtcgagcag ggtcaccgcc tccggcacct tgcggatgtc gtccttgatc
    27181 tccgccagcc ccaccagcgc ctcgggattg ttgggctgca cctcgaccca gcgggtgagc
    27241 gcggcttcgg cgcggtcgta gcggcgcgcc ccgagcgcga ggatgccctg gttcttgtag
    27301 agttcgccca aagcggcggg cggcgccgcg ccgctctcga tcatcgcttc gatcgccgag
    27361 gcctgcatgg cgcggtcggt ggtttcgagg gcgaggcgca gctccatcga ggcggcgaga
    27421 tagcggacct cgccgctgcg cgcggccgac cgcgccgccg ccgccgcgct tgccgccgcg
    27481 ggataattac gctgctggag cgcggtctgg agcgccgcca gcgcctcgcg ctcggctttg
    27541 ctgagggtcg gcaccgccgg cgccgcggcg ccggcctggg cgtgagcggg ggcgcccgcg
    27601 gcggcaaggc cgccgagcgc cagcgacacg ccgagcgcga tgctggaaac cgaattcata
    27661 aggtgcctcc tgtttgccgc cagggcatgg cggcccgtct cgacgggcgc gatagctggg
    27721 cttctatcgc caatcgaaac ccctttctag agcccacgca ctgaaccgcg cttgaactgc
    27781 aaacctctgc gaattctcga caaatggctg ggctttcggc gcggtttgac ggcgtctccc
    27841 gggctggcta ggcacccgcg atgattgcca tcctctcgcc ggccaagacg ctcgactata
    27901 agcggccggt gccgccggtg cgcccgaccg agcccgcctt cgccgacgac gccgccaagc
    27961 tcgcgcgctc ggccgcgcgg cttccgcgcg cccggctgat ggagctgatg cacatttcca
    28021 aggagcttgc cgcgctcaac gccgaccgct tccgcggctt tgcggaccag cccgagcggg
    28081 cggccatcta cgcctttgcc ggcgacgtct ataccgggtt cgaggtgcac agcctcgatg
    28141 aagaggcggt gctgttcgcg caggaccatg tccgcatcct gtccggcctc tacggcctgc
    28201 tccggccgct cgacgcgatc cgcccctacc ggctcgaaat gggcaccaga tgggcgccgc
    28261 gccgccggga cctttatgct tattgggggc cgcgcatcgc gcaggcgctg gaccgggatg
    28321 tggcggcgca gggcgacctc attgtgatca atctcgccag caaggaatat tggcgcgcgg
    28381 tcgaaagccg gctcgggccc ggggcgcggg tgatcgagat cgattttcgc gaggaggggc
    28441 cggccggcct ccggttcaac accttcgccg ccaagcgcgc ccgcggcatg atggcgcgct
    28501 acatttgcga gcacaggctc gacgatccgg aggcgctgaa ggcgttcggc agcgacggct
    28561 atgccttcgt gcccgagcaa tcggatgcgc agcgctggcg cttcagccga tcgtgaccat
    28621 ataggaaacg cctcgcgcgc gtacgcgagg tggcgctcgc cgatcgcatc ggctaggctc
    28681 cgtccgaacc gccgcgggac cccgcggcgc ctttgtccat aagctttagg aagacccatt
    28741 ggccagcgca ccgccgacca acgacagccc caccggcgcc gacatcacgc ccatctccat
    28801 cgtcgaggag atgaagacct cctatctcga ctatgcgatg tcggtgatcg tcagccgcgc
    28861 gctcccggac gtgcgcgacg ggctgaagcc ggtccaccgg cgcatcctct acgcctgcca
    28921 ggaaggcgga ttcgtccccg gccgcccgta ccgcaagtcg gccaagatcg tcggcgacgt
    28981 gatgggcaat taccacccgc acggcgacgc ttcgatctac gacgcgctcg cccggctcgc
    29041 gcaggattgg gcgatgcggg tgccgctgat cgacggccag ggcaatttcg gatcgatgga
    29101 ccccgatccg ccggcgtcga tgcgctatac cgaggcgcgg ctcgccaagg tcgcgatgac
    29161 cctgctcgag gatatcgacc gcgacacggt cgacttcagc cccaattacg acggatcgcg
    29221 cgaggagccg caggtgctgc ccgcgcgctt cccgaacatc ctcgtcaacg gcgccggcgg
    29281 catcgcggtg ggcatggcga ccaacatccc gccgcacaat ctcggcgagg tgatccgcgc
    29341 ctgcaaagcc tatatggacg acaaggagat caccatcgag cagctgatgg agatcatccc
    29401 cggcccggac ttcccgaccg ctcctttgat cctcggccag gccggcgccc gcagcgccta
    29461 ccataccggc cgcggctcga tcatcatgcg cgcccgccac gtggtcgagg aaggccgcgg
    29521 cgaccggcgc tcgatcgtcc tgaccgcgat cccctaccag gtcggcaaga acggcctcgt
    29581 cgagaagatc gccgaggccg ccaaggacaa gcgcatcgag ggcgtctccg acattcgcga
    29641 cgaatccaac cgcgagggcg tgcgggtggt gatcgacctc aggcgcgacg ccacgcccga
    29701 cgtggtgctg aaccagctgt ggcggcacac gcccgcccag tcgagcttcc cggccaacat
    29761 gctcgccatc cgcggcggcc ggccggagac gctgacgctc aaggacatca tcgaggcgtt
    29821 cgtcaaattc cgcgaggaag tgatcacgcg ccgcgccaag ttcgacctcg ccaaggcgcg
    29881 cgagcgggcc cacatattgc tcggcctcgt cattgccgtc accaacctcg acgaagtggt
    29941 gcggatcatc cgcggctcgg cctcgcccgc cgaagcgcgc gccgccttgc tcgcccgcga
    30001 atggccggtc gccgagatcg cgtcctatat cgcgctcgtc gaagcggtcg agcatgaggt
    30061 cgacggcgac agctatcaac tcagcgaagc gcaggtccgc gccatcctcg agctgcgcct
    30121 ccaccgcctg accggcctcg gccgcgacga gattgccggc gaattgcgcg agctcgccac
    30181 ctcgatcggc gaattgctcg acatcctcgg caaccgcgtc aagctctacg aagtgatgcg
    30241 cggcgagttc gacgagatcg ccgcccagtt cgccaccccg cgcatgaccg agatcgcgcc
    30301 ggcctgggac gggatcgagg acgaggatct gatcgagcgc gaggacatgg tcgtgaccgt
    30361 gacgctcggc ggctacatca aacgcacccc gctcgacacc ttccgcgccc agaagcgcgg
    30421 cggcaagggc cgcgccggca tggcgaccaa ggcggaggac gccgtcacca acctgttcgt
    30481 gacctcgacc cacacgccgg tcctgttctt ctcgacccac ggcaaggtct accgcctcaa
    30541 ggtctggcgg ctgcccgagg gggcaccgca ggcgcgtggc cggccgatgg tgaacctgct
    30601 gccgctcgcc gagggcgaga ccatctccag cgtgctgccg ctgcccgagg acgaggcgga
    30661 gtggaagaac ctccacatca tgttcgcgac ggcgaagggt atcgttcgcc gcaacagcat
    30721 ggacgccttc acccgggttc cgaccgcggg caagatcgcg atgcgcttcg gcgagagcga
    30781 cgagagcgag gacatgtccg accgcctgat cggagtctcg ctgctcaccg aagaggatga
    30841 cgtgctgctc gccaccaagt gcggcaaggc gatccgcttc gccgccaccg acgttcgcga
    30901 gttccagagc cgcaccgccg ctggcgtccg cggggcccgg ctccaggatg gcgacgaggt
    30961 catctcgctc tcgatcctca agggcttccg ggcttccacc gaggagcgcg acgactatct
    31021 ccgccacgcg ccttggcgcg aacgggcgga ggatgccgcc cctcccccac cccgcgacat
    31081 gtcggacgag cgcttcgagc ggttccgggc ggcggaggag ttcatcctca ccgtctgcgc
    31141 caacggctat ggcaagcgct cctccgccta cgaatatcgg cgcatcaacc gcggcggcca
    31201 ggggatcacc aacatcggca acctcgatcg caacggcccg gtcgtcgcca gcttcccggc
    31261 ccataatggc gagcagctga tgctggtgac ggaccaggcg aagcttatcc ggatgtcggt
    31321 gggagacacc cgggtcatcg gccgcggctc ggcgggcgtg cgcctgttcc acgtcgcgcc
    31381 gggcgagcat gtcgtctccg ccgcccggat cgaggagagc gaggaggaag ccgaagccgt
    31441 ggtcgacgtc gaggatccga tgctgcaagc gagcgacgcg gtgatctcgg gggccgagga
    31501 ctaagaatcc tccccggctt aagctggacc accggttccg gtcctggctg gacagcaatt
    31561 cctccccaca atttcgtggg gagggggacc accgcagggt ggtggagggg ccactcgccg
    31621 gggttcgcaa agactcctcg aaccaagctc ccgtagacat cgcgcgagtc cgggaccgtg
    31681 cgtgccaccg cgccaggctt ccccagccgc cgcgatgacc gggccgccgc cggcgcggca
    31741 acccggctct gggcccctgc cttcgcaggg gcacgatgct ctcgcttctg gcgacgttgg
    31801 cagcaggcgc atgggagggt ttgccgtcct tgtgcccctg cgaaggcagg ggcccagacc
    31861 gggcgaagcc cgcgatggaa gccggatcga ccctgccccg caatttaccc cttgcgcatc
    31921 actcgctccc ctgttatttc tgaaattaca gaaagagcag acgatgcccc tgatcgacca
    31981 tcccgacgcg aaagccttca tcctccactg gggggagatg gggacgcaat ggggggtcaa
    32041 ccgctcggtc tcccagatcc acgctttgct ctatctttcc gatcggccgc tgaccgccga
    32101 cgagatcgtc gaacagctca atctcgcccg ctccaacgtc tccaccgggc tcaaggagct
    32161 gcagggctac ggcatcgtgc gccgggtcca tgtcgagggc gaccggcgcg accatttcct
    32221 ggccgagacc gacctgtggg acatgctgat gcggatcacg gccgagcgca agcggcgcga
    32281 gatcgacccg acgctcgccc tgctcggcga gcttgcggcg cggctgaagc aggatgaatc
    32341 cgcgccgcgc catgtccgcg agcggatcac ccgcatgcac gaattcatct cgacgctcgg
    32401 caattggtac gaccaggtcc gcctgctgcc caagcccacg ctcgtcactt tgatgaagct
    32461 cggcgccagg gtcgcccgct tcatccccgg cgccgccaaa cccaagcagc cataaaggac
    32521 aggagcatga cccgcaccag atgacccacg cccgtcttct tctggaatta cagaattaac
    32581 cgtcgaaagg aaaatcctca tggtcgagat cgcctatctc ctctacctcc tcatctccat
    32641 cggcatcacc atcctggtgg cgcggaccct ctccaggaat ggcgaggtgt tcctggtgca
    32701 atgcttcggc cataacgaga cgctcgcaag gtccaccaac catctgctgg tggtcggctt
    32761 ctacctggtc aatatcggct tcatcaccct gacgttgagc atcggcgcgg agcccaccac
    32821 gcttcccgag gcgatccgtt tcctcagcag caaggtcgga ctcgccgtgc tcgtgctggg
    32881 cggcatgcac ttcttcaaca tgaccgggat cgcccatttc ggccgcaagg tgagcgagtg
    32941 gttcgccgtc cagcaggagg cgcgcccgct cgcctgatgc accaaccggg cgggcctcgg
    33001 cccgcccctt ccctctggct gaaggagccg gccgatgtat ggacgcaagc ccgcaggtcg
    33061 cacgctgatg gtcgcctgcc tcgtcgcagg ccctaccttc acctccacct ttgcgctcgc
    33121 caatgcctat ttgaagctgc cccagccgat cctgctgacc gtgccggaga tcgcgccgct
    33181 cctggccttc tgcctggcgg cgaccgcctt cggcttcgtg atcgcgctgg tccccaccgg
    33241 tctgggaacg ctgctgatga gcagcctcgg cgaccatgtg cccgcggccc ggacgccctt
    33301 ggcctggacg ctggccggtg cggccatcgg ctacggcctg gcgatgctga cgggagcctg
    33361 gcccggtttg ccgcccgccg ccttcgccct cgtcgccacc tccgcgctct gcgcccgcat
    33421 ctgccggggc ggggcggagt gggagtgagc gtgcgaagcg cacctcgtca tgccggactt
    33481 gatccggcat cccgctcctt ccctcctcgt ccggtgagtt gaagaaggga gatcccggat
    33541 caggtccggg atgacg
//
LOCUS       DASVRK010000113        13932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_269279, whole genome shotgun sequence.
ACCESSION   DASVRK010000113 DASVRK010000000
VERSION     DASVRK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269279"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="VF589_04165"
     CDS             <1..2032
                     /locus_tag="VF589_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX8256803.1"
                     /translation="EACREIRARCPHVHISGGLSNLSFSFRGNEPVRRAMHSVFLYHA
                     IPAGLDMAIVNAGQLDVYDTIDGELREACEDVILNRTPKNGADATERLVTLAERYRGS
                     DPVQEKLAAEWRGWPVAKRLEHALVKGIDAHVVEDAEEARQSAARPIEVIEGPLMDGM
                     NVVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPFIEEEKEKSGATQGKGRIVMATVKG
                     DVHDIGKNIVGVVLQCNGFEVIDLGVMVPWQAILDAANENNADMIGLSGLITPSLDEM
                     VTVAAEMQRIGLTLPLLIGGATTSRVHTALRIDPAYEGPVIHVLDASRAVGVASSLVS
                     DTQRDPLVEKTKQDYRDIAAARANKGQNELSPLAEARANGFAFDPSGQAPPPARPGMH
                     HFGEWPLRDLRTTIDWTPFFRAWELAGNYPAILEDPVVGESATALFADAQAMLDRMIA
                     EHWVTPRGTVGLWRCKREGDDVLVLAGNDWTRLPFLRQQVKKREGRPNMCLADFISPE
                     GEDWIGGFAVSIHGLEPHLARLKAENDDYSDILLKALADRLAEAFAERLHAHVRESVW
                     GYAGVEHLTNQELIRERYIGIRPAPGYPACPDHSLKPILFDMLGGNPAGVELTENFAM
                     LPTSAVSGFYFGHRDADYFGVARIGRDQLEDYAARRGVSLEQAERWLRPNLD"
     gene            2317..3252
                     /locus_tag="VF589_04170"
     CDS             2317..3252
                     /locus_tag="VF589_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8256804.1"
                     /translation="MKTGLPLLAALLLAGPAAAQPSGKPFFVQEKNKSYASLQRAVDA
                     IGGGRGTILIAAGSYSDCAVQDAGHVAYAAREPGTVVLDGGICEGKATLVLGGRSARV
                     EGLTFKNLRVPDRNGAGIRIEKGDLHIVETLFTDSENGILSARFPGGTITVERSTFSG
                     LGGCEDGHGCSHGIYVGEYGRLIVRSSRFERGTGGHYLKSRTPLVEITDSSFDDSAGR
                     ATNYMIDLSNGATGLIARNMFVQGRDKENYSAFIMVAAEQRANRSAGLTVTDNEAAIA
                     PGVERSSSFVADKSGDPIRIENNRLGRGVEQFARR"
     gene            complement(3610..3996)
                     /locus_tag="VF589_04175"
     CDS             complement(3610..3996)
                     /locus_tag="VF589_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256805.1"
                     /translation="MRLALLLPALLAAGLAGASFAKSSADNPAWMAGEWNMMSADEPK
                     RFRDCNSDHSDWFDPDGKFTSVGDATGRWSVKGDKLIMVTTDPGDGNPEAKGEAYTIR
                     FKRVAPDELRFSDGEGQWKMVRCPDK"
     gene            complement(4072..5760)
                     /locus_tag="VF589_04180"
     CDS             complement(4072..5760)
                     /locus_tag="VF589_04180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8256806.1"
                     /translation="MNQYRGLPSLLWRGVLALSLLFASPAMADTLVDNVNGYTAARGG
                     EIVRFTGLLVGDDGRVKQLLTRRDKRPERPRYRLDGRGRTLIPGLIDAHGHVMGLGMG
                     ALQLDLSDTNSLQEAQARIAAFARANPSPRWIIGRGWNQERWGLGRFPTAADIDTVVS
                     DRPVWLERVDGHAGWANSVAMKEAGVTAASQSPPGGKIETAGGQPSGIFIDAASELVG
                     KVVPPPLPRVRDRAFAAAQDILLANGITSVADMGTSGEDWAVIRRAGDLGRLNVRILS
                     YAAGVDPLLAVAGTGPTPWLYDSKLRMVGVKLYSDGALGSRGAWLKQPYRDSPGERGL
                     AFLSDATLKNLMSRAAMDGFQVAVHAIGDAANAQLLEGIEDMSDTYKGDRRWRVEHAQ
                     IVDPADLPRFARNGIIASMQPVHEASDWQMAEARMGRERLGGAYAWQSMARAGVPLAL
                     GSDFPVESPNPFHGLAVAVSREDARGQPAGGWQPEQKLSIAQAFAGFTRGGAFASFAE
                     DRLGTLERGKLADFIFIDRDIFAVAPREIRGTRVLETWVGGRKVWEPGRTTLGR"
     gene            complement(5757..6623)
                     /locus_tag="VF589_04185"
     CDS             complement(5757..6623)
                     /locus_tag="VF589_04185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8256807.1"
                     /translation="MARIAFIGLGVMGAPMARHLAAAGHSLSLFNRSSEKARLWVSRH
                     GGSFAATPAAAAGNADAVIACVGTDEDVEQITLGRDGAFRNMKEGSLFIDHTTVSAKI
                     ARRVAEEGHKLGIACVDAPVSGGQAGAEHGQLSVMCGGTEEAVRAAEPLMKAYAARIV
                     HVGPDGSGQLTKMVNQLAIAGVLQGLSEALVFAQASGLDTAKVFEAVSGGAAQSWQMV
                     NRWKTMAEGEFDFGFAVDWMRKDLGLALDEARTNGASLPLAALVDQFYAEVQAMGGGR
                     QDTSSLIRRLKK"
     gene            6713..7951
                     /locus_tag="VF589_04190"
     CDS             6713..7951
                     /locus_tag="VF589_04190"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX8256808.1"
                     /translation="MAAIADRPELPVSLDDIRAAAARIAGAVVRTPTMLSRTLSDATG
                     AEIWIKFENLQFTAAYKERGALNKLLLMDPAIRARGVIAASAGNHAQAVAYHGARLGV
                     PVTIVMPRPTPTIKVMQTEGHGASVLLHGELFDEAYEEAQRIAGERGLTFVHPFNDPH
                     IIAGQGTVALELLEDAPAIDTLLVPIGGGGLISGMAIAAKALQPELEIIGAQAELYPS
                     MYNAIRGESRPSSGDTIAEGIAVKEPDRLTTAIIRQLVDEILLVPERDIETAVSLLLQ
                     IEKTVAEGAGAAGLAALLTHPDRLKGRKIGLVLTGGNIDTRLLATVLLRDLARSGRLA
                     RLRIQLQDRPGALFSVIKLFEKHQINIIEVYHQRLFTTLPAKGLYTDIECEARDRAHL
                     QRLMDDLAGAGFNFHPVEIH"
     gene            8079..8840
                     /locus_tag="VF589_04195"
     CDS             8079..8840
                     /locus_tag="VF589_04195"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX8256809.1"
                     /translation="MSAPFRFPRFFVTTPSPCPYLPGKTERKVFTELSGQHAVELNDA
                     LGRIGFRRSQGVAYRPSCVDCGACISVRVIASEFQPSATQRKLIRRHSDLEVTACKPW
                     TTEEQYGLLRRYLRQRHPGGGMVDMDESDFADMVEQSPVKTYVIEYREPSVDGRPGKL
                     VGACLTDQQADGLSMIYSFFEPDCEARSGLGTYIILDHIVRAARAGLPYVYLGYWVED
                     SKRMAYKAKFRPLERLGPDGWRRFEPEQKELPLTR"
     gene            complement(8963..9322)
                     /locus_tag="VF589_04200"
     CDS             complement(8963..9322)
                     /locus_tag="VF589_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX8256810.1"
                     /translation="MSLADAYQDLRRFGLGLPEAGEEAPWGHCALKVRNKTFVFLNLD
                     EAELSLSVKLPASRDFALIFDWAEPTGYGLGRSGWVSGRFGLADAIDRDLLKRWIAES
                     YRAVAPKKLAALVPGEA"
     gene            complement(9412..10113)
                     /locus_tag="VF589_04205"
     CDS             complement(9412..10113)
                     /locus_tag="VF589_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8256811.1"
                     /translation="MRVLLIEDEPTTAKSIELMLTSEGFNVYTTDLGEEGLDLGKLYD
                     YDIICLDLNLPDMHGYDVLKKLRVAKVGTPVLILSGISEMDSKVRALGFGADDYVTKP
                     FHRDELVARIHAVVRRSKGHSQSVIKTGKLAVNLDAKTVEVDGARVHLTGKEYAMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLSLACSGDNYIETVWGRG
                     YVLRDPEDMEQARVA"
     gene            10284..10940
                     /locus_tag="VF589_04210"
     CDS             10284..10940
                     /locus_tag="VF589_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX8256812.1"
                     /translation="MWDKYVLFLDGEAIVIDKPAGLAVHPGSRTPESLEDYLHHFRFG
                     FERLPLPVHRLDRDTSGCLLLARNPKAHKRFQRAFEEKMVAKTYVAVLDGVPDAEAGI
                     VDMPLGKVSTREEGWRIVHDPQGKAAVTHWQVRTVKDGRAVVVFTPETGRTHQIRVHA
                     AAGIGFSIAGDPVYGAGRGPMLLHALSLRVDRAPKPPVEATAPLPPAFVNAGFGDVVL
                     "
     gene            10927..11340
                     /gene="arfB"
                     /locus_tag="VF589_04215"
     CDS             10927..11340
                     /gene="arfB"
                     /locus_tag="VF589_04215"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX8256813.1"
                     /translation="MSSSEAYAVPEEALSETFLASSGPGGQNVNKVATACQLRCDVFK
                     LGLSPPVYARLKELAGSRMTSAGEIVITARSYRTQEANREDARARLAELIAKAHVRQP
                     KRIKTKVSRAAKARRVEAKSQRGAIKAARAKPRLD"
     gene            11436..11927
                     /locus_tag="VF589_04220"
     CDS             11436..11927
                     /locus_tag="VF589_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8256814.1"
                     /translation="MYDFRIEAADKGEAYRQIIAAADALTAGEPDAIANMANISALLW
                     ESLPDLNWAGFYRNVGGELVLGPFQGRAACIRIPFGKGVCGAAAATREVQCIDDVHAF
                     PGHIACDSASASELVVPIVLDGELIGVLDLDSPLRARFDREDAEGCSKLVQEVAARLR
                     SRS"
     gene            12012..13022
                     /locus_tag="VF589_04225"
     CDS             12012..13022
                     /locus_tag="VF589_04225"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8256815.1"
                     /translation="MKKLAIVILAATALVPPASAQTAAPAARAAAPARMTAPARMAPA
                     PGVTWRQRGPMATPRVLTRPTAPRGQPHAGRPHRPGAGSGHGFHRGGDNRVVGRGPHR
                     GPRFRHIRRFQHGFILPHFWFGPQFHVSNWGLYGFSQPYGDQRWIRHYDDAYLIGGDG
                     RIHDSRYGMDWDSYGERWNYGEGGGPPMYGDESPYPPEGDYGYGGGHGQGGYPDGGHG
                     GHGGGGHAGGGPGAAGGYGPPPGTGPGHGGGACQMTYSDGGPPPPPPPCAHGAGWGYG
                     WGTMVVTETTTTTTTGGPSVTKHSYHVEEAPAPSKRMYRPAPSKRKYRPAPPPPSPGE
                     RG"
     gene            complement(13019..13474)
                     /locus_tag="VF589_04230"
     CDS             complement(13019..13474)
                     /locus_tag="VF589_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8256816.1"
                     /translation="MGSYLVVMDDSQEARAALRFAARRAARTGSTIELLAVIPQQEFV
                     QWGGVQAAMEEEARLRAEAMIAQASGAIMEEAGLKPSITVREGDPVKAVTEMLGECEN
                     IAALVLGAAAEGNPGPLVSHFAGTAAGSLPCPLMIVPGNLSEETLDRLS"
     gene            13638..>13932
                     /locus_tag="VF589_04235"
     CDS             13638..>13932
                     /locus_tag="VF589_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8256817.1"
                     /translation="MPIELKMPALSPTMEEGTLAKWLKQEGDKVVSGDVIAEIETDKA
                     TMEFEAVDEGVIAKILVAEGTDEVKVGTVIAILAEEGEDVSAAGSSATPAPPRQ"
BASE COUNT         2265 a         4989 c         4503 g         2175 t
ORIGIN      
        1 cgaagcgtgc cgcgagatcc gggcccgctg cccgcacgtg catatctcgg gcgggctttc
       61 gaacctcagc ttctcgttcc ggggcaacga gccggtgcgc cgggcgatgc actcggtgtt
      121 cctctaccat gccattccgg ccgggctcga catggcgatc gtcaatgccg gccaattgga
      181 cgtctacgac accatcgatg gggagctgcg cgaagcctgc gaggacgtga tcctcaaccg
      241 caccccaaag aacggcgccg atgcgaccga gcggctggtg accctggccg aacgctatcg
      301 cgggtccgat cccgtccagg agaagctcgc cgccgaatgg cgcggctggc cggtcgccaa
      361 gcggctcgaa catgctttgg tgaagggcat cgacgcgcat gtcgtcgaag atgccgagga
      421 agcgcgccaa tccgccgccc gcccgatcga ggtgatcgaa ggcccgctga tggacggcat
      481 gaacgtggtc ggcgacctgt tcggatcggg caagatgttc ctgccccagg tggtcaaatc
      541 cgcccgcgtg atgaagaagg cggtcgccca cctcattccc tttatcgagg aggagaaaga
      601 gaagagcggc gccacccagg gcaagggccg gatcgtgatg gcgacggtca agggcgacgt
      661 ccacgacatc ggcaagaaca tcgtcggcgt cgtgctccag tgcaacggct tcgaagtgat
      721 cgacctcggc gtgatggtgc cgtggcaggc gatcctcgat gccgccaacg agaacaatgc
      781 cgacatgatc ggcctgtccg ggctgatcac gccctcgctc gatgagatgg tcaccgtcgc
      841 cgccgagatg cagcgcatcg gcctcacctt gccgctgctg atcggcggcg ccaccaccag
      901 ccgggtccac accgcgcttc gcatcgaccc ggcttatgaa gggccggtga tccacgtgct
      961 cgacgcgagc cgcgcggtcg gcgtcgcctc ctcgctggtc tccgacaccc agcgcgaccc
     1021 gctcgtcgag aagaccaagc aggattatcg cgacatcgcc gcggcccgcg ccaacaaggg
     1081 ccagaacgag ctttccccgc tggccgaggc ccgcgccaac ggcttcgcgt tcgacccgtc
     1141 cggccaggcg ccgccgccgg cgcgtcccgg catgcatcat ttcggcgaat ggccgctgcg
     1201 cgaccttcgc accaccatcg attggacgcc cttcttccgc gcctgggagc tggccggcaa
     1261 ttaccccgcc atcctcgaag acccggtggt cggcgaaagc gccaccgcct tgttcgccga
     1321 cgcgcaggcc atgctcgacc gcatgatcgc tgagcattgg gtgacgcccc ggggcacggt
     1381 cggcctgtgg cgctgcaagc gcgaggggga cgatgtgctg gtgctcgccg gcaacgactg
     1441 gacccggctc cccttcctcc gccagcaggt gaagaagcgc gaggggcggc ccaacatgtg
     1501 cctcgccgac ttcatctcgc cggaaggcga ggactggatc ggcggcttcg cggtctcgat
     1561 ccacgggctc gagccgcacc tcgcccgcct caaggcggag aatgacgatt attccgacat
     1621 ccttttgaag gcgctggccg accggctcgc cgaagccttc gccgagcggc tccacgccca
     1681 tgtccgcgag agcgtgtggg gctatgccgg cgtcgagcac ctcaccaacc aggagctgat
     1741 ccgcgagcgc tatatcggca tccgcccggc gcccggctac cccgcctgcc ccgaccacag
     1801 cctgaagccg atcctgttcg acatgctcgg cggcaacccg gccggggtcg agctcaccga
     1861 gaatttcgcg atgctgccga cctcggccgt gtcgggcttc tatttcggcc accgcgacgc
     1921 cgattatttc ggcgtcgccc gcatcggccg cgaccagctc gaagactatg ccgcccgccg
     1981 cggcgtctcg ctcgaacagg cggaaaggtg gctgcggccc aacctcgatt gatctccagc
     2041 gaactccgtt cgtgtcgagc gaagtcgaga cacccctccg agcgcagcca agaatgagcg
     2101 ggttgagggc ttccccgcag cctcaggacc ccccgggggc atggccgttc atagcctcgg
     2161 cttcgctcgg cagggtccct cgacttcgct cgggacgaac ggtttaactt tgcccagtca
     2221 ttacatctgg tgccggttcc gtcccacagc ggatcagccc cgcattaacc ccgattagcg
     2281 gtcgactccc gctttcgcct cagtcacgtc gataccatga aaaccggcct cccccttctc
     2341 gccgcccttc tcctagccgg ccccgccgcc gcgcagccgt caggcaagcc cttcttcgtc
     2401 caggagaaga acaaaagcta tgccagcctg cagcgcgcgg tcgacgcgat cggcggcggg
     2461 cgcggcacca tcctgatcgc ggcgggcagc tatagcgact gcgcggtcca ggatgcgggc
     2521 cacgtcgctt atgccgcgcg cgagcccggc acggtcgtgc tcgacggcgg catttgcgag
     2581 ggcaaggcga cgctcgtgct gggcggccgc tcggcccgcg tggagggcct taccttcaag
     2641 aacctgcgcg tgcccgaccg gaacggcgcc gggatccgga tcgaaaaagg cgacctccac
     2701 atcgtcgaga ctttgttcac cgacagcgag aacggcatcc tctccgcccg ctttcccggc
     2761 ggcaccatta ccgtcgaacg ctcgaccttc tccggccttg gcggctgcga ggacgggcat
     2821 ggctgctcgc acggaatcta tgtcggcgaa tatggccggc tgatcgtgcg cagctcgcgc
     2881 ttcgagcgcg gcaccggcgg ccattatctg aagagccgca cgccgctggt cgagatcacc
     2941 gattcgagct tcgacgacag cgccgggcgc gcgaccaact acatgatcga cctgtccaac
     3001 ggcgccaccg gcctcatcgc ccgcaacatg ttcgtccagg gccgcgacaa ggagaattac
     3061 agcgccttca tcatggtcgc ggccgagcag cgggccaacc gctcggccgg cctcaccgtc
     3121 accgacaacg aggccgcgat cgcgcccggc gtcgagcgca gcagcagctt cgtggccgac
     3181 aagagcggcg acccgatccg gatcgagaat aaccgcctcg gccgcggcgt ggagcaattc
     3241 gcgcggcgtt agggggcgtc ccatcgatgg ggaagcccgt cattccagcg aagctggaac
     3301 cccccttctt cttccccggc tgccaaagaa aagtgagatg ccaggtttcg ctggcgtgac
     3361 gatcacttcc caaacggaca atcggcggaa gcgatcaggc ctccgcgccc ttcgcccagg
     3421 cgaagaaggt ggtgacgagg catagcgcca gcttgagctt gttcatctga cttcccgata
     3481 cgtgccgcct ggagcagctt cactatagaa ggcggtggtt acgggaatcc tcatgcgggc
     3541 gcttgatttc ctcccgatga caatattcca gccggcggcg ggtggcagag aattgtccgc
     3601 cgcgatgcct cacttgtcgg ggcagcgcac catcttccac tgcccctcgc catcggagaa
     3661 gcgcagctcg tcgggagcga cccgcttgaa gcggatggta taggcctcgc ccttggcctc
     3721 tgggttgcca tcgcccggat cggtggtgac catgatcagc ttatccccct tgaccgacca
     3781 gcggccggtc gcgtcgccga cgctcgtaaa cttgccgtcg gggtcgaacc agtcgctatg
     3841 atcgctgttg cagtcgcgga atcgcttcgg ctcgtcggcc gacatcatgt tccactcgcc
     3901 ggccatccat gcggggttgt cggcggagga cttggcgaaa gaggccccgg ccagcccggc
     3961 ggcaagcaaa gcgggcagca gcagggcaag gcgcatggcg gatctccgag gcgttcaggt
     4021 ccggccctgg cggccgtccc cccggcgtaa gcggccgaag ctagcgtccc gttaacgccc
     4081 gagcgtggtc cggcccggct cccacacctt ccgcccgccg acccaggttt cgagcacgcg
     4141 cgtgccgcgt atctcgcgcg gcgccacggc gaagatgtcg cggtcgatga agatgaagtc
     4201 ggcgagcttg ccgcgctcga gggtgccgag ccggtcctcc gcgaaggaag cgaaagcgcc
     4261 gccgcgcgtg aagccggcaa aggcctgcgc gatgctgagc ttctgctcgg gctgccagcc
     4321 gccggccggc tggccgcgcg catcctcgcg gctcaccgcc actgcgaggc cgtgaaaggg
     4381 attgggcgat tcgaccggga agtcggatcc cagcgccagc ggcacgccgg cgcgggccat
     4441 gctctgccag gcataagcgc cgccgagccg ctcgcggccc atccgcgctt cggccatctg
     4501 ccagtcggaa gcttcatgga ccggctgcat cgacgcgatg atgccgttgc gggcgaagcg
     4561 gggcaggtcg gcgggatcga caatctgcgc atgctcgacg cgccagcgcc ggtcgccctt
     4621 ataggtgtcg gacatatcct cgatgccttc gagcaattgc gcgttggcgg cgtcgccgat
     4681 ggcgtggacc gcgacctgga agccatccat cgccgcccgg ctcatcagat tcttgagcgt
     4741 ggcgtcgctg aggaaggcga ggccgcgctc gccgggggaa tcgcgatagg gctgcttcag
     4801 ccaggcgccg cgcgagccga gcgcgccgtc gctgtaaagc ttgacgccga ccatgcgcag
     4861 cttgctgtcg tagagccagg gcgtgggccc ggtgccggcg accgccagca acggatcgac
     4921 gccggcggca taggagagga tgcgcacgtt gaggcggccg aggtcgccgg cgcggcggat
     4981 caccgcccaa tcctcgccgc tagtgcccat gtcggcgacc gaggtgatgc cgttggcgag
     5041 caagatgtcc tgcgcggcgg caaaggcgcg gtcgcgcacc cgcggcagcg gcggcggcac
     5101 gaccttgccc accagctccg aggccgcgtc gatgaagatg ccgctcggct gcccgcccgc
     5161 cgtctcgatc ttgccgcccg gcggcgactg gctggcagcg gtgacgccgg cttccttcat
     5221 cgcgacggaa ttggcccagc cggcatggcc gtcgacccgc tccagccaga ccggccggtc
     5281 ggaaacgacc gtgtcgatgt cggccgcggt cgggaagcgg ccgaggcccc agcgttcctg
     5341 gttccagccg cgcccgatga tccagcgcgg gctgggattg gcgcgggcga aggcggcgat
     5401 ccgggcctgc gcctcctgca gcgaattggt gtcgctgagg tcgagctgga gcgcgcccat
     5461 gccgagcccc atgacatggc cgtgggcgtc gatcagaccg gggatcaggg tgcggccgcg
     5521 gccgtcgagc cggtagcgcg gccgctcggg ccgcttgtcg cgccgcgtca gcagctgctt
     5581 cacccgcccg tcgtcgccca ccagcagccc ggtgaagcgg acgatctcgc caccccgggc
     5641 ggcggtgtag ccgttgacat tgtcgaccag ggtgtcggcc atcgccggcg aggcgaacag
     5701 cagcgacaag gccagcactc ccctccacaa cagggagggg aggccacgat attggttcat
     5761 tttttaagcc ttctgatcag cgacgacgtg tcctggcggc cgccgcccat cgcctgcact
     5821 tcggcgtaga attgatcgac caaagccgcc agcggcaggc tcgccccgtt ggtccgcgct
     5881 tcgtcgagcg cgaggccgag atccttgcgc atccagtcga ccgcgaagcc gaagtcgaac
     5941 tcgccctcgg ccatcgtctt ccagcgattg accatctgcc agctctgcgc cgcgccgccc
     6001 gaaaccgctt cgaacacctt ggccgtatcg agcccggaag cctgggcgaa gaccaaagcc
     6061 tccgacagcc cctgcagcac gccggcgatg gcaagctggt tgaccatctt ggtgagctgg
     6121 ccgctgccgt cggggccgac atggacgatc cgcgccgcat aggccttcat cagcggctcg
     6181 gcggcgcgga ccgcctcctc ggtgccgccg cacatcaccg acagctggcc atgctcggcg
     6241 ccggcctggc cgcccgacac cggcgcgtcg acgcaggcga tgccgagctt gtgcccttcc
     6301 tcggccaccc gccgcgcgat cttcgccgac accgtcgtat gatcgatgaa cagcgagcct
     6361 tccttcatgt tgcggaaggc gccgtcgcgg ccaagcgtga tctgctcgac atcctcgtcg
     6421 gtgccgacgc aggcgatcac cgcatcggca ttgcccgccg ccgccgccgg ggtggccgcg
     6481 aagctgccgc cgtgccgcga gacccataag cgggccttct cgctgctccg gttgaacagg
     6541 ctgaggctgt gccccgccgc cgccagatga cgcgccatcg gcgcccccat cacgccgagc
     6601 ccgataaatg ctatccgtgc catgacattg gcgcataagc cttgttcgcc cgccgcgcca
     6661 gatggcatgc cggacggcag acagggccgg cttcgcatgg tagagcgccg ccatggccgc
     6721 tatcgcagac cgaccggagc tccccgtctc gctcgacgac ataagggccg ccgccgcccg
     6781 catcgccggc gcggtggtgc gcaccccgac gatgctcagc cgcaccctgt cggacgcgac
     6841 cggcgcggaa atctggatca agttcgagaa cctccagttc accgccgcct acaaggagcg
     6901 cggcgcgctc aacaaattgc tgctgatgga cccggcgatc cgcgcgcgcg gggtgatcgc
     6961 cgcctccgcc ggcaaccacg cccaggcggt ggcctatcat ggcgcccggc ttggcgtgcc
     7021 ggtcacgatc gtgatgccgc gccccacccc caccatcaag gtgatgcaga ccgagggcca
     7081 cggcgccagc gtcctcctcc acggcgagtt gttcgacgag gcctatgaag aggcgcagcg
     7141 catcgccgga gagcgcggcc tgaccttcgt ccacccgttc aacgacccgc acatcatcgc
     7201 cggccagggc acggtcgcgc tcgaactgct cgaggacgca cccgcgatcg acaccttgct
     7261 ggtgccgatc ggcggcggcg gcctcatctc gggcatggcg atcgccgcca aggcgctgca
     7321 gccggagctc gagatcatcg gcgcccaggc cgagctctat ccgagcatgt acaacgcgat
     7381 ccgcggcgag agccggccct cctcgggcga caccatcgcc gagggcatcg ccgtcaagga
     7441 gccggaccgg ctgaccaccg ccatcatccg ccaattggtc gacgagatcc tgctggtccc
     7501 cgagcgcgac atcgagaccg cggtcagcct gctcctccag atcgagaaga cggtggccga
     7561 gggggccggg gcggccgggc tggcggcttt gctgacccac cccgaccggc tcaagggccg
     7621 caagatcggt ctcgtcctca ccggcggcaa tatcgacacg cggctgctcg ccaccgtgct
     7681 gctgcgcgac ctcgcccgct ccggccgcct cgcgcgcctg cgtatccagc tccaggaccg
     7741 cccgggcgct ttattctcgg tgatcaagct gttcgaaaag caccagatca acatcatcga
     7801 agtctatcac cagcggctgt tcacgaccct gcccgccaag ggcctctata ccgacatcga
     7861 atgcgaggcc cgcgaccgcg cccatctcca gcggctgatg gacgacctcg ccggcgccgg
     7921 cttcaatttc caccccgttg agattcactg acccagccgt cccgcgctgg aacggctcat
     7981 cgcttcgggc gtatttcacc gcgggcgccc ttcctcggcc gctccggtac gcataagatc
     8041 atgccaggct gagtcgtccc ccccggactg gagtttcggt gagcgcaccc tttcgttttc
     8101 cccgcttctt tgtgacgacg ccgtcgcctt gcccgtattt gccgggcaag accgagcgga
     8161 aggtgttcac cgagctcagc ggccagcatg cggtcgagct caacgacgcg ctggggcgga
     8221 tcggcttccg ccgcagccag ggcgtcgcct accggccgag ctgcgtcgat tgcggcgcct
     8281 gcatctcggt ccgggtgatc gcctccgaat tccagccgag cgccacccag cgcaagctga
     8341 tccgccgcca ttccgacctc gaagtcaccg cctgcaagcc gtggacgacc gaggagcaat
     8401 atggcctgct ccgccgctac cttcgccagc gccatcccgg cggcggcatg gtcgacatgg
     8461 acgagagcga tttcgccgac atggtcgagc agagcccggt caagacctac gtcatcgaat
     8521 atcgcgagcc gtcggtggac ggccgccccg gcaaactggt cggcgcctgc ctcaccgacc
     8581 agcaggccga cggcctgtcg atgatctaca gcttcttcga gcccgattgc gaggcgcgca
     8641 gcggccttgg cacctacatc atcctcgacc atatcgtgcg cgccgcccgc gccggcctgc
     8701 cctatgtcta tctcggctat tgggtcgagg attcgaagcg catggcctac aaggccaagt
     8761 tccgcccgct cgagcgcctc ggccccgacg gctggcgccg cttcgagccc gagcagaaag
     8821 agctgccgct gacgcgctga acgaaggatc cgtcaccttc accgtccgcc atccacgtca
     8881 cctgaacttg tttcagggtc catgcctcca cccgcgccgc ggctcgaaca tggatgctga
     8941 aacgagttca gcatgacccg ctctacgctt cccccggcac cagcgccgcg agcttcttcg
     9001 gagcgacggc gcgatagctc tcggcgatcc agcgcttcag caggtcccgg tcgatcgcgt
     9061 cggccaggcc gaagcgtccg ctcacccagc cgctgcggcc gaggccgtag ccggtcggct
     9121 cggcccagtc gaagatcaag gcgaagtcgc ggctcgccgg cagcttcacc gacaggctga
     9181 gctcggcttc gtcgaggttg aggaacacga aggtcttgtt gcggaccttg agcgcgcaat
     9241 ggccccacgg cgcctcctcg cccgcctcgg gaagcccgag gccgaagcgg cgcagatcct
     9301 gataggcgtc ggcaaggctc atcggccgcc ttcatgcacc aggcggaccc gcgcgcaaag
     9361 cgaagccggc gccgcatcgc tgcagcgccg gcgcccactt ttcgcttggg atcaggcgac
     9421 ccgcgcctgc tccatatcct cgggatcgcg cagcacgtag ccgcggcccc acaccgtctc
     9481 gatataattg tcgccgctgc aggccagcga cagcttcttg cgaagcttgc agatgaacac
     9541 gtcgatgatc ttcagttccg gctcgtccat cccgccatac aggtggttga ggaacatttc
     9601 cttggtgagc gtggtgccct tgcgcaggct caagagctcc agcatcgcat attccttgcc
     9661 ggtgaggtgc acgcgggcgc cgtcgacctc gaccgtcttg gcgtcgaggt taaccgccag
     9721 cttgccggtc ttgatcaccg actggctgtg gcccttggag cggcgcacga ccgcgtggat
     9781 gcgggcgacc agctcgtccc ggtggaacgg cttggttaca taatcgtcgg cgccgaagcc
     9841 caacgcccgc accttggaat ccatctcgct gatgcccgac aggatcagca ccggcgtgcc
     9901 gaccttggcg acccggagct tcttcagcac gtcatagccg tgcatgtcgg gcaggttcag
     9961 atcgaggcag ataatatcgt aatcgtagag cttgccgagg tccaacccct cctcgcccag
    10021 gtccgtcgta tagacgttga agccttcgct ggtgagcatc agctcgatgc tcttggcggt
    10081 ggtcggctcg tcttcaatca gcagtacacg catggtctcg ccccttgctc gcgcttcccc
    10141 gccgttcggt ctttggaagc cggtaacttt gattaatcat gcgataagtg aacttaaaag
    10201 gttaattcgg cgtaaacgcc tgaaacgccg cgagtcgcgg cgcgatcgtt gcgccgatgc
    10261 aacagccgtg gcagggcgga gacatgtggg ataaatacgt cctgttcctc gatggcgagg
    10321 cgatcgtgat cgacaagccg gccgggctgg cggtgcatcc gggctcgcgc acgcccgaga
    10381 gccttgaaga ttatcttcac cacttccgtt tcggtttcga gcggctgccg ctccccgtcc
    10441 accggctcga ccgcgacacg tccggctgcc ttttgctcgc ccgcaacccc aaggcgcaca
    10501 agcgcttcca gcgcgccttc gaggagaaga tggtggcgaa aacctacgtc gccgtgctcg
    10561 acggggtgcc ggacgccgaa gcgggcatcg tcgacatgcc gctcggcaag gtgtcgacgc
    10621 gggaggaggg ctggcgcatc gtccacgatc cccagggcaa ggcggcggtg acgcactggc
    10681 aggtccggac ggtgaaggac ggacgcgcgg tggtggtgtt cacgcccgag accgggcgca
    10741 cgcaccagat ccgggtccac gccgccgcgg gcatcggctt ctcgatcgcc ggcgacccgg
    10801 tttacggcgc gggccgcggg ccgatgctgc tccatgcctt gtcgctccgg gtggaccgcg
    10861 cgcccaagcc gccggtcgag gcgacggcgc cgctgccgcc ggccttcgtc aatgcgggtt
    10921 tcggcgatgt cgtcctctga agcctatgcc gtcccggagg aggcgctgag cgagaccttc
    10981 ctcgcctcga gcgggccggg cgggcagaat gtcaacaagg tcgccaccgc ctgccagctg
    11041 cgctgcgacg tgttcaagct cggcctctcc ccgcccgtct atgcgcggct caaggaactt
    11101 gccggcagcc gcatgaccag cgccggcgag atcgtcatca ccgcgcgcag ctaccgcacc
    11161 caggaggcca atcgcgagga cgcccgcgcc cggctcgccg agctgatcgc caaggcgcat
    11221 gtccgccagc cgaagcggat caagaccaag gtcagccgcg ccgccaaggc gcggcgggtc
    11281 gaggccaagt cgcagcgcgg agcgatcaag gccgcccgcg ccaagccccg cctggattga
    11341 acgacagccg tcattccagc gaaagctgga atctcccttc ccccttccta cttgcagcat
    11401 gactgaccgg cccccgccgg acgaccggag gaccaatgta cgatttcagg atcgaagccg
    11461 ccgacaaggg cgaagcctat cgccagatca tcgcggcggc cgatgcgctg accgccggcg
    11521 agcccgacgc catcgccaac atggcgaaca tctcggcgct gctgtgggaa agcctgcccg
    11581 acctcaactg ggccggcttc taccgcaatg tcggcggcga gctggtgctg gggccgttcc
    11641 agggccgcgc cgcctgcatc cgtatcccgt tcggcaaggg cgtgtgcggc gccgccgccg
    11701 cgactcggga ggtgcagtgc atcgacgacg tccatgcctt tcccggccac atcgcctgcg
    11761 acagcgcctc ggcctcggaa ctggtggtgc cgatcgtcct cgacggcgaa ttgatcggcg
    11821 tgctcgacct cgacagtccg ctgcgcgcgc gcttcgaccg cgaggatgcg gagggctgtt
    11881 ccaaactggt acaggaggtc gccgcgcgcc tccgctcccg tagctaaaag cccctcagcg
    11941 agttacaaaa gtcggactgg ccgaggcgcg tcccgccccg gccggatggt ttcgcgaaca
    12001 ggggatgaac catgaagaag ctcgcgattg tgattctggc cgcaaccgca ttggtgccgc
    12061 cggcctctgc gcagaccgcc gcaccggcgg cgcgcgcagc cgctcccgcc cggatgacgg
    12121 ccccggcccg gatggcgccg gcgccgggcg tcacctggcg gcagcgcggc ccgatggcga
    12181 cgccgcgcgt cctgacccgt ccgacggccc cgcgcgggca gccccatgcc ggacggccgc
    12241 accgcccggg cgccggctcc ggccacggct tccatcgcgg cggcgacaat agagtggtcg
    12301 gccgcggccc gcaccgcggc ccgcgcttcc ggcacatccg ccgcttccag cacggcttca
    12361 tccttccgca tttctggttc ggaccgcaat ttcacgtctc gaactggggc ctttacggct
    12421 tttcccagcc ctatggcgac cagcgctgga tccgccatta tgacgatgcg tatctgatcg
    12481 gcggcgacgg ccgcatccac gacagccgct acggcatgga ctgggattcc tacggcgagc
    12541 gctggaatta tggcgagggc ggcggcccgc cgatgtacgg cgacgagagc ccttacccgc
    12601 ccgagggcga ttacggctat ggcgggggcc acggccaggg cggctatccc gacggcggcc
    12661 atggcggcca tggcgggggc ggccatgccg gcggaggccc cggcgcagcc ggcggctatg
    12721 gcccgcctcc cggcaccggc cccggtcatg gcggcggcgc ctgccagatg acctatagcg
    12781 acggcggtcc gccgcctccg ccgccgccct gcgcgcacgg cgccggctgg ggctatggct
    12841 ggggcaccat ggtggtgacc gagaccacca cgaccaccac caccggcggc ccgagcgtca
    12901 ccaaacattc ctatcatgtc gaggaagcgc cggccccgtc caagcggatg taccggccgg
    12961 ccccgtcgaa acggaaatac cggccggccc cgccgccgcc tagccctggc gagcgcggct
    13021 agctcagccg gtcgagcgtc tcttcgctga gattgcccgg cacgatcatc agcgggcacg
    13081 gcaacgaacc ggccgccgtg cccgcgaaat ggctgaccag cggcccggga ttgccctcgg
    13141 ccgcggcgcc gagcaccaga gcggcgatat tctcgcactc gcccagcatc tcggtgaccg
    13201 ccttgacggg gtcgccctcg cgcaccgtga tcgagggctt gaggcccgct tcctccatga
    13261 tcgcgcccga ggcttgggcg atcatcgctt cggcgcggag ccgcgcctcc tcctccatcg
    13321 ccgcctgcac gccgccccat tggacgaact cctgctgcgg gatcaccgcg agcagctcga
    13381 tcgtgcttcc ggtccgcgcc gcgcgccggg ccgcgaagcg cagcgccgcc ctcgcttcct
    13441 ggctgtcgtc catgacgacg aggtaagacc ccatttcgcc ccctaggtga atgcgcgcga
    13501 acggtgcccc ggatcgctcc cccacgcaag gcttgcccct ccgccgccgg aaccgccgcg
    13561 ctcaggacaa gcttgacccg gcccctcatc ttgctgtttg gagcggggcg caccccggcc
    13621 ggaaagcgag cattcccatg cccatcgaac tcaagatgcc agccctctcc ccgaccatgg
    13681 aggaaggcac gctcgccaaa tggctgaagc aggagggcga caaggtcgtg tccggcgacg
    13741 tcatcgccga gatcgagacc gacaaggcga cgatggagtt cgaagcggtg gacgaaggcg
    13801 tgatcgctaa gatcctggtc gcagaaggca cggacgaagt gaaggtcggc accgtcatcg
    13861 ccatcctcgc cgaggaaggt gaggacgttt ccgctgccgg ttcttcagcc acgcccgctc
    13921 ccccgcggca gg
//
LOCUS       DASVRK010000114         3889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_206969, whole genome shotgun sequence.
ACCESSION   DASVRK010000114 DASVRK010000000
VERSION     DASVRK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206969"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..769)
                     /locus_tag="VF589_04240"
     CDS             complement(143..769)
                     /locus_tag="VF589_04240"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8256818.1"
                     /translation="MKTILSVAALAGLLILAAQAQGERHAAATESGPSSPATDMSGWT
                     RPLGAAPADRAAAPLDQPAPDAAEPDVVDAHWMALTMWGEARGQGEEAMRAVGHVIAN
                     RRRSGLHGDFVTDTVSAAFQFSCWNPGDPNREAMANVLDLPEGSREHDLWRASRRIAD
                     EILAGRSADPTAGALFYHTTGVAPRWSRGIQPVRRIGTHLFFTRAATA"
     gene            1149..1664
                     /gene="rplJ"
                     /locus_tag="VF589_04245"
     CDS             1149..1664
                     /gene="rplJ"
                     /locus_tag="VF589_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX8256819.1"
                     /translation="MDRAQKAEAVAALKQTFNEASAVVITRNLGLTVAQSTALRNRMR
                     EAGARYKVAKNTLALIAVEGTAYAPISEMLTGPTALATSSDPVAAAKVAVDFAKTTDR
                     FEIVGGAMGDTLLDVAGVRALAELPSLDQLRATIIGLIQAPASKIARTINEPGAMLAR
                     VIGAYAAKEDA"
     gene            1720..2091
                     /gene="rplL"
                     /locus_tag="VF589_04250"
     CDS             1720..2091
                     /gene="rplL"
                     /locus_tag="VF589_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX8256820.1"
                     /translation="MADINTLVDQLSELTVLQAADLAKALEERWGVSAAAAVAAAPAA
                     GPAAEAAEEQTEFDVILVNDGGKKINVIKEVRAITQLGLGEAKALVEGAPKAVKEGIS
                     KAEAEELRKRLEEAGATVELK"
     gene            complement(2177..3658)
                     /locus_tag="VF589_04255"
     CDS             complement(2177..3658)
                     /locus_tag="VF589_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8256821.1"
                     /translation="MSEPAGGDAVANRRVHPATIPLRFLKEAPSTLLGIPAAFAFISD
                     YGVSEVLLFAAAAALLGVTFQWLAWRRFRYGIGERDLVIESGIVSRTRRSIPFDRIQD
                     VDIERGPLARLFGLAKLRIETGGSGRDEGVLDSVTLAEAERLRDALRAARRTGAAVEA
                     GPDEAPQAALLFQMGVPRVLAAGLFNFSMVYLAGMFALLQAFDRWLPFDIYDPGRWVG
                     LVGNRFGDRWSVGAIAAVALLALLLGTLTGVARTLARDFGFRLTLEGTGTAVGFRRER
                     GLFTRSEVMLPKRRIQLALAETGPLKRLLGWHRLACQTLGGGRPGESGRQSLAPLARR
                     PEIDSILTALDGMRIAEAGELNIVSSRHFLRRLATAVLLPAIVIVAIAFAMPAALLAF
                     ALLPALAAIAFFERRFHRFGLLDGKLYVQAGLWRQRQWIVPLANIQSLRLSRSWLQRR
                     LDLATLSIDTAGGSALDPPRIIDLREGPARALLQALRTAAAAP"
     gene            complement(3655..>3889)
                     /locus_tag="VF589_04260"
     CDS             complement(3655..>3889)
                     /locus_tag="VF589_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8256822.1"
                     /translation="LHIGRGVWTKTETIVPLARVQHIDVSQGPLERAFGISRLILHTA
                     GTMHSLVVLPGLARGTAEAMRDDIRARIRRDEA"
BASE COUNT          644 a         1332 c         1373 g          540 t
ORIGIN      
        1 gccgccgaag tcgcgggcgc ctgatcggat caaagtggta ctttgatccg gggtaaaaga
       61 agaggccggg gcggcgacgc tccggccttt ttgctgtcag ttccccggcg aaagccgggg
      121 tccagtcctt ccggcggggg cttcaggccg tcgccgccct cgtaaagaag aggtgggtgc
      181 cgatgcggcg aacgggttgg atgccgcgcg accagcgcgg agcgacgccg gtggtgtggt
      241 agaagagcgc gccggcggtg ggatcggcgg agcggccggc gaggatctcg tcggcgatcc
      301 gccgggacgc gcgccacagg tcatgctcgc ggctgccctc gggcaaatcg agcacgttcg
      361 ccatcgcctc gcggttggga tcgcccgggt tccagcaact gaactggaag gccgcgctga
      421 ccgtgtcggt gacgaagtcg ccatggaggc cgctacgccg ccggttggcg atgacatggc
      481 cgaccgcgcg catcgcctcc tcgccctgcc cgcgcgcctc gccccacatg gtgagcgcca
      541 tccaatgcgc gtccaccaca tccggttcgg cggcgtcggg ggcgggctga tcgagggggg
      601 cagcggcccg atcggcgggc gcggcaccga gcgggcgggt ccagccgctc atatccgtgg
      661 caggggagga agggccggat tcggtggcgg cggcgtgacg ttcgccctgc gcctgcgcgg
      721 cgaggatgag cagcccggcc aaggccgcga ccgagagaat cgtcttcatc ggctccaccc
      781 gcttccaatg cgaacgcctt agcctgctga tattacaatg ggaagctcag gcccggtagg
      841 cgcgtgcctg gcagttgaaa ttcaacttcc ggttgattcg cagggcggct gcccatatag
      901 gcgcgtcact gaccgtgtgg cttcggcccc ggtcgccgtc cgagacagtt ggtgggggcc
      961 ttggcttcct taatctccag cctagacggg gaaagcgttt ttgccgggcg ccgagtgcgt
     1021 cccggttgcc gaatttgtct tcggcctgaa ccttccccct tcggacaatg cggcgcgcgc
     1081 ccaaacgtgc gtgtcgtcaa tccccgcgct gcggatggtc cgccgcgcga ctgtgtgaag
     1141 gaacaggcat ggatcgagct caaaaggccg aagccgtcgc cgcgctgaag cagacgttca
     1201 acgaggcgag cgcggtggtg atcacccgca acctcggcct cacggtggcc cagtccaccg
     1261 ctctgaggaa ccggatgcgc gaggccggcg cccgctacaa ggtcgccaag aacacgctcg
     1321 ccctcatcgc cgtcgagggc accgcttacg cgccgatcag cgagatgctg accggcccga
     1381 ccgcgctcgc cacttcgtcg gatccggtcg cggccgccaa ggtcgcggtc gatttcgcca
     1441 agaccaccga ccggttcgag atcgtcggcg gggcgatggg cgataccttg ctcgacgtcg
     1501 ccggagttcg ggcgctcgcc gagctgccgt cgctcgacca gctgcgcgcc accatcatcg
     1561 gcctcatcca ggctccggct tcgaagatcg cccgtacgat caacgagccg ggcgcgatgc
     1621 tggcccgcgt gatcggcgcc tatgcggcca aggaagacgc gtaaatttca acactgacgc
     1681 ttcccggctt ctgccggaac atcaaaacgg agacttaaaa tggcagacat caacaccctc
     1741 gtcgaccagc tcagcgagct gaccgtcctt caagccgccg acctcgccaa ggcgctcgaa
     1801 gagcgttggg gcgtttcggc cgccgcggcc gtcgccgccg ctcccgccgc cggcccggcc
     1861 gccgaggctg ccgaagagca gaccgagttc gacgtgatcc tcgtcaatga cggcggcaag
     1921 aagatcaacg tgatcaagga agtccgcgcg atcacccagc tcggcctcgg cgaagccaag
     1981 gcgctggtcg agggcgctcc gaaggccgtc aaggaaggca tttccaaggc cgaagccgag
     2041 gagctccgca agcgcctcga ggaagccggc gcgaccgtcg agctgaagta aaagcctccg
     2101 gacttcgtcc ggggcgaaca gcgaaggggc gggtcccagg ccggggcccg ccccttttcg
     2161 cgtccgcggc cggcggtcag ggcgccgcgg ccgcggtgcg aagcgcctgc agcagggcgc
     2221 gggcggggcc ttcgcgcaag tcgataattc ggggcgggtc caaagccgat ccacccgccg
     2281 tgtcgatcga cagggtcgcg agatcgagcc ggcgctgcag ccacgaccgg ctcaggcgca
     2341 gcgactggat gttggcgagc gggacgatcc actggcgttg ccgccacagc cccgcctgca
     2401 cgtacaattt gccgtcgagg aggccgaagc ggtggaagcg ccgctcgaaa aaggcgatcg
     2461 cggcaagcgc cggcagcaag gcgaacgcca ggagcgccgc cggcatcgcg aaagcgattg
     2521 cgacgatgac gatcgcgggc agcagcacgg cagtcgcgag ccgccggaga aagtggcggc
     2581 tcgacacgat attgagctcg cccgcctcgg cgatgcgcat gccgtccagc gccgtgagga
     2641 tggaatcgat ctcgggccgc cgcgccagcg gagccaggct ctgccggccg ctctcgccgg
     2701 gccggccgcc gcccagcgtc tggcaggcca gccggtgcca gccgagcagc cgcttcagcg
     2761 gcccggtctc cgccagcgcg agctggatgc gccgcttcgg gagcatcact tcgctgcgcg
     2821 tgaacaggcc gcgctcgcgg cggaagccga ctgccgtccc cgtcccctcc agcgtcagcc
     2881 gaaagccgaa atcgcgcgcc agcgtccgcg ccacgccggt gagcgtgccg agcagcaagg
     2941 cgagcagggc gacggcggcg atcgcgccga cgctccagcg gtcgccgaag cggttgccga
     3001 ccagcccgac ccagcggccg ggatcgtaaa tgtcgaacgg cagccagcgg tcgaacgcct
     3061 gcagcaaggc gaacatgccg gcgagataga ccatcgagaa attgaacaat cccgccgcca
     3121 gcacccgcgg cacccccatc tggaacagca aggcggcctg gggcgcctcg tcaggccccg
     3181 cctccacggc cgctccggtc cgccgcgcgg cgcggagcgc gtcgcggagc cgttcggcct
     3241 cggcgagggt gacgctgtcg agcacgcctt cgtcgcggcc cgacccgccg gtctcgatcc
     3301 tgagcttggc gagaccgaac aggcgggcga gcggcccgcg ctcgatatcg acgtcctgga
     3361 tgcggtcgaa cgggatcgag cgccgggtgc ggctcactat gccgctttca atgacgaggt
     3421 cgcgctcgcc gatcccgtag cggaaccggc gccaggccag ccactggaac gtcaccccca
     3481 gcaatgccgc cgcggcggca aagaggagca cttcgctgac gccgtaatcc gagatgaagg
     3541 cgaaggctgc gggaatgccg agcaaggtcg agggcgcttc cttgaggaag cggagcggga
     3601 tggttgcggg atggacccgc cggttcgcga ccgcgtcgcc gcccgccggt tcgctcacgc
     3661 ttcgtcccgg cggatgcggg cgcggatgtc gtcgcgcatc gcctccgccg tgccgcgggc
     3721 gaggccgggc aggacgacca ggctgtgcat cgtgccggcc gtgtggagga tcagccgcga
     3781 gattccgaac gcgcgctcga gcggcccttg cgagacgtcg atatgctgaa cccgcgccag
     3841 cggcacgatt gtctcggtct tggtccagac gccgcggccg atgtgcagc
//
LOCUS       DASVRK010000115         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_580435, whole genome shotgun sequence.
ACCESSION   DASVRK010000115 DASVRK010000000
VERSION     DASVRK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580435"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VF589_04265"
     CDS             complement(<1..603)
                     /locus_tag="VF589_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8256823.1"
                     /translation="MADADPRRRRSSKVMPLPECVALVLQGGGALGSYQAGVYEALCE
                     AAIEVDWVAGISIGAINAAIIAGNPPERRVPRLRQFWEGATAELPSFPFWPGDLGREW
                     LHEWSAGFIALAGAPGFFRPRPVPPTFAAPGTAGALSFYDSAPLARTLDELVDWDLVN
                     DGRVRLSVGAVEVESGNFHYFDSRDERLDARHVMASGALPP"
     gene            716..1618
                     /locus_tag="VF589_04270"
     CDS             716..1618
                     /locus_tag="VF589_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX8256824.1"
                     /translation="MRFGGRRISDNVGSQRGQGGGGFGFPMGGGGGMPFGGGGLGCGG
                     IVILIILAFVFGINPLSMMGGGGGGQSVAPHQTSKAGRGGAQEICNSDEVTRFSCQVL
                     ASTEDQWTRIFQAAGQRYEPAEMIFYARSGQSGCGAAQSAMGPFYCPSDQNIYLDTDF
                     FRELQTRFKAPGDFAQAYVIAHEVGHHIQTITGISDKVRRAQSRASEAEGNALQVRME
                     LQADCYAGVWAAAEREAMEPGDAEEGLRAAEAIGDDTLQKAAQGYVVPESFTHGTSAQ
                     RQEWLQRGLRSGDPAQCDTFGNLI"
     gene            complement(1647..>2918)
                     /locus_tag="VF589_04275"
     CDS             complement(1647..>2918)
                     /locus_tag="VF589_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8256825.1"
                     /translation="RLQHRFSDSLSVTSRLLYGDYDKLYANVFPAAPVTADPVTGRPR
                     FGVEAYRDGTQRRNLLSQTDLVWQVDTGGIGHVLLAGFEYADQETGSQRINGAFATGD
                     PASPLTSRAAVPLTDPVVTPPVAAWTVARSTEGRADALAFYVQDQVSLGERFDVVAGL
                     RYDRFELEVDNLLNGDRFRRTDDLWSPRLGLVWKPVEPLSVYGSYSRSYLPQSGDQFA
                     SLDLTSAALRPERFDNYEVGLKWDIRPALSLTATLYRLDRTNSRAVDPSSGDVVLTGA
                     QRSRGVEIGLAGAITERWQVSGGYTLQEAKIRRATTACDPAARDCEVPLVPRHQFALW
                     SRYDLSSRIGFGVGLYHQSKSFASISNEVVLPGFTRVDAAAYFTISEGIQAQINIENL
                     FGEKYFATAHNDNNITPGAPTRALATLRFGF"
BASE COUNT          462 a          947 c         1095 g          414 t
ORIGIN      
        1 cggcggcaac gcgccgctgg ccatgacgtg gcgcgcatcg aggcgctcgt cgcggctgtc
       61 gaaataatgg aagttgccgc tttccacctc gaccgcgccg accgaaagcc gcacccggcc
      121 gtcattgacc agatcccagt cgacgagctc gtcgagcgtg cgggcgagcg gcgccgaatc
      181 gtagaagctg agcgcaccgg cggtgcccgg tgcggcgaag gtcggcggga ccgggcgggg
      241 ccggaagaag ccgggcgcgc cggccaaggc gatgaagccc gccgaccatt cgtgcagcca
      301 ctcgcggccg agatcgcccg gccagaaggg gaagctcggc agctcggcgg tggcgccctc
      361 ccagaactgg cgcagccgcg gcacccgccg ctcgggcggg ttgccggcga tgatcgccgc
      421 gttgatcgcg ccgatcgaga tgcccgccac ccaatcgacc tcgatcgcgg cttcgcacaa
      481 ggcttcgtag acgccggcct ggtaggagcc gagcgcgccg ccgccctgga ggacgagcgc
      541 gacgcattcg ggcaaaggca tgactttgga ggagcgccgg cggcgcggat cggcatcggc
      601 catggccgcc tatgtgccag ccgaccggcg gcatcaaggg gctgccgttt cggccatgca
      661 accagccggg cggccgcgca ttgggcgtgg gtcggtatcg gtggagtaag ggcttatgcg
      721 cttcggcgga cgcaggatca gcgacaatgt cggcagccag cgcggccagg gcggcggcgg
      781 cttcggcttc ccgatgggcg gcggcggcgg catgccgttc ggcggcggcg gcctgggctg
      841 cggcggcatc gtcatcctca tcatcctcgc cttcgtgttc ggcatcaatc cgctgagcat
      901 gatgggcggc ggcggcggcg gccagagcgt ggctccgcac cagaccagca aggccggccg
      961 cggcggcgcg caggagattt gcaatagcga tgaggtgacg cgattctcct gccaggtgct
     1021 ggcctccacc gaggaccaat ggacccggat cttccaggcg gccgggcagc gctacgagcc
     1081 cgccgagatg atcttctatg cccgcagcgg ccaatcgggc tgcggcgcgg cgcaatcggc
     1141 gatgggcccc ttctattgcc cgtccgacca gaacatctat ctcgacaccg atttcttccg
     1201 cgagctccag acccgcttca aggcgcccgg cgatttcgcg caggcctatg tgatcgcgca
     1261 tgaggtcggc caccatatcc agaccatcac cggcatttcc gacaaggtgc gccgcgcgca
     1321 gtcgcgggcg agcgaggccg aggggaacgc gctgcaggtg cggatggagc tgcaggcgga
     1381 ttgctatgcc ggcgtctggg cggcggccga gcgcgaggcg atggagcccg gcgacgccga
     1441 ggaagggctg cgcgccgccg aagcgatcgg cgacgacacg ctgcaaaagg cggcgcaggg
     1501 ctatgtcgtc cccgaaagct tcacccacgg cacgtccgcc caacgccagg aatggctcca
     1561 gcgcggcctc cgcagcggcg atcccgcgca atgcgacacg ttcggcaatt tgatctgacc
     1621 ggcgcgggcc gccgttccgg cgggacctag aagccgaagc gcagcgtcgc cagcgcgcgg
     1681 gtcggcgcgc cgggggtgat gttgttgtcg ttatgggcgg tggcgaaata cttctcgccg
     1741 aacaggttct cgatgttgat ctgagcctgg atgccctcgc tgatcgtgaa ataagcggcc
     1801 gcgtcgaccc gcgtgaagcc gggcagcacg acctcgttgg agatcgaggc gaagctcttc
     1861 gactgatggt agaggccgac gccgaagccg atccggctcg acaggtcgta gcggctccac
     1921 aaggcgaact ggtggcgcgg caccagcggc acctcgcagt cgcgggcggc ggggtcgcag
     1981 gcggtggtgg cgcggcggat cttcgcctcc tgcagcgtgt agccgccgct cacctgccag
     2041 cgctcggtga tggcgccggc gaggccgatc tcaacgcccc ggctgcgctg cgcgccggtg
     2101 agcaccacgt cgcccgagct cgggtcgacc gcgcggctat tggtgcggtc gagccggtag
     2161 agcgtcgcgg tgaggctgag cgcggggcgg atgtcccact tgagcccaac ctcgtaattg
     2221 tcgaaccgct cgggccgcag cgccgcgctg gtgaggtcga gcgaggcgaa ctggtcgccg
     2281 gactggggga ggtaggagcg gctgtagctc ccgtacacgg agagcggctc gacaggcttc
     2341 cagaccagcc cgagacgcgg cgaccacaga tcgtcggtgc gccggaagcg gtcgccgttg
     2401 agaaggttgt cgacctccag ctcgaagcgg tcgtagcgca ggcccgcgac gacgtcgaaa
     2461 cgctcgccca acgacacctg gtcctgaacg tagaaagcga gcgcgtcggc gcgcccttcg
     2521 gtggagcggg cgaccgtcca ggcggcgacg gggggcgtca cgacggggtc ggtgagcggc
     2581 acggcggcac ggctcgtcaa ggggctggcg ggatcgccgg tggcgaaggc gccgttgatg
     2641 cgctggctgc cggtctcctg gtcggcatat tcgaagccgg cgagcagcac atggccgatg
     2701 ccgccggtgt cgacctgcca caccagatcg gtctggctga ggagattgcg gcgctgggtg
     2761 ccgtcgcgat aggcctcgac cccgaagcgc ggccggccgg tgacgggatc ggcggtcact
     2821 ggagcggcgg ggaagacgtt ggcgtagagc ttgtcatagt cgccgtagag cagccggctg
     2881 gtgacgctga ggctgtccga gaatcggtgc tggagccg
//
LOCUS       DASVRK010000116        12754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_518404, whole genome shotgun sequence.
ACCESSION   DASVRK010000116 DASVRK010000000
VERSION     DASVRK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518404"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..313
                     /locus_tag="VF589_04280"
     CDS             29..313
                     /locus_tag="VF589_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256826.1"
                     /translation="MKPIHMAFGAAATLVAALALGGWSPAAAQAQGSRIKGKTPQNFP
                     YEIRNGRRVPKAQRVVAADGSWREEVRDGPCMTIRERTAQGEYREVRKCD"
     gene            392..1165
                     /locus_tag="VF589_04285"
     CDS             392..1165
                     /locus_tag="VF589_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256827.1"
                     /translation="MHMIRTGEGPKLLLVHGLGSSAASWAPIAPALAQGRELIAIDLP
                     GHGETPAEGDSGTFTGLARSLEAFLDEQGLTGVDAVGLSLGGRLVLEMARRGRVGAIV
                     ALDPGGFWEGWERSYLRVTLGASVWLVRRLGAALPALAAAPPSRAMLLAQFSNRPWDV
                     PAGEAARELGTIAATPTFDALLDDLAFGPVQAGPAAAGAGPVTIGWGRDDRLCLPVQA
                     LRAQEAFPSAQLHWFDDCGHFAIWDKPRQVIELIRNSTG"
     gene            1278..1838
                     /locus_tag="VF589_04290"
     CDS             1278..1838
                     /locus_tag="VF589_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX8256828.1"
                     /translation="MKSIILAATVAAIGLPAVSAAAQTPSQTNREYQRDVNDARRDYQ
                     RDMRDADNRGERREAREEYREEMRDARQDRREDLRDWRRYRSYDYNRLEPGQQRYYAD
                     RYYRSGNYYQPRRLGHGERIYRGNDGRYYCRRDDGTTGLIVGGLAGGVLGAEVAPGDS
                     QVLGTILGGAAGALLGRALDREVTCH"
     gene            complement(1892..2269)
                     /locus_tag="VF589_04295"
     CDS             complement(1892..2269)
                     /locus_tag="VF589_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256829.1"
                     /translation="MLNRAKAAGLAAMLMLALAGCDGGGEPANTTKVKASSPYVEQLR
                     TLSELNRGLALRRAIQDTGQDCKKVDRSGYQEDYKNMSMWTVRCSDGNDWSLFIAPNG
                     DVQVRSCTQTAQLGLPACRFEGA"
     gene            complement(2281..2787)
                     /locus_tag="VF589_04300"
     CDS             complement(2281..2787)
                     /locus_tag="VF589_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256830.1"
                     /translation="MPPSLPHLSIALLALAACSGDSRTADPPPESELGSAISEPRMPA
                     ATPRPAPAVAAPAPGGNSAEAAAATLRTYYRLIEAGRYEAAWQLRERPAKRGDASAFA
                     RAFSGYSQYRATIGAPSPVVEAGGSLYVEVPVQTYGVTRQGKPFSSAGTVTLRRPDKQ
                     GEWRIYGG"
     gene            complement(2798..3904)
                     /locus_tag="VF589_04305"
     CDS             complement(2798..3904)
                     /locus_tag="VF589_04305"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEX8256831.1"
                     /translation="MVPFRRFLHALAADIERDIDRIEDLAAAGEGLDRKVRLLAYAGY
                     RNSERLRLKGRIVRYRKPLDAGEGMLTRLRAMMEIYNSHELPGIAVRLDGYGGTHDSV
                     TDEEGYFTFDLPVPLPLPERTAWEQVTLSTPGREAQAASVQVPVMAPGADNHWGIISD
                     IDDTVVETGATDFVKNWRRVLVDRPQDRLAVPGASSLYKTIAGDHVAPVRPFFYVSSS
                     PWNLYGFIAEFMELNGIPHGPMFLKDYGIDADQLVGKSHDAHKLAAIETVLAFYPGFR
                     FLLIGDNGQRDVAIYARVVQDFGSRVAGVFIRDVDGSCRSGPEGELLAGIEAAGIPTF
                     CGAGFDDAVAVAKALNLDRPLEAAKAVVEAAPKA"
     gene            4007..4228
                     /locus_tag="VF589_04310"
     CDS             4007..4228
                     /locus_tag="VF589_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256832.1"
                     /translation="MGNKHLRPDETPLDLIDDLVRNPGIGQSKGVFARAGGGDSDLIQ
                     GENTVEGDRENDTDLGGGIDKYDLDRNGV"
     gene            complement(4291..5592)
                     /gene="hslU"
                     /locus_tag="VF589_04315"
     CDS             complement(4291..5592)
                     /gene="hslU"
                     /locus_tag="VF589_04315"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX8256833.1"
                     /translation="MNEKLTPKAVVAALDAHIVGQRDAKRAVAVALRNRWRRQQLSDA
                     LREEVTPKNILMIGPTGCGKTEISRRLAKLADAPFVKVEATKFTEVGYVGRDVEQIAR
                     DLVEEAVRLEKERRRIAVKDAAEEAAMERLLDALTGTGASEATRASFRQRFHDGHLAA
                     SEIEIEVSETPATPFEIPGMGGQVGMINLSDMMSKALGQAPKKRRRMKVAEAWPRLVE
                     EEADKRLDTDDVNRAALADAEANGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIE
                     GTTVATKYGPMKTDHILFIASGAFHVAKPSDLLPELQGRLPIRVELKALTEEDFVHIL
                     KDTEASLTDQYRALIATEGVDVSFTDDGIRAIARIAAEVNGQIENIGARRLQTVMEKL
                     LEDVSFEAEDRRGEKIEVDAAYVDRQLAEVARNTDLSKYVL"
     gene            complement(5631..6173)
                     /gene="hslV"
                     /locus_tag="VF589_04320"
     CDS             complement(5631..6173)
                     /gene="hslV"
                     /locus_tag="VF589_04320"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX8256834.1"
                     /translation="MESWHGTTILSVRKNGHAVVAGDGQVTLGQTVIKPNARKVRRLG
                     DGSVIGGFAGATADAFTLFERLERKLEQHHGALMRAAVELAKDWRTDKYLRNLEAMMI
                     VADKEVTLILTGNGDVLEPQDGVAAIGSGGNFALAAARALDEYEADAETIARKAMKIA
                     AEVCVYTNDNLTVEKLETAR"
     gene            complement(6261..7232)
                     /locus_tag="VF589_04325"
     CDS             complement(6261..7232)
                     /locus_tag="VF589_04325"
                     /inference="COORDINATES: protein motif:HMM:NF018389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEX8256835.1"
                     /translation="MLALLLAAAEPAPLKTFSDWAVGCDNGRLCQAVGLVPEDDLDAA
                     TMMVLRRPEAGAEPRVAFSMAAEGAPWVEVDGRRFKALDVASDDGDTAVPPQARAFLA
                     ALLAGKKAALIGPDGKPVGTLSTAGSSAALLYIDDKQGRVGTVTALTRRGDKPASAVP
                     PPPPLPVVQAPPASSKPARMASAAAVAAARDDMTCETPAEEEAAQAHRLDDRHSLVLV
                     PVKCGSGAYNFLSVALVLDEAGKARPGEFEREKGGETNWLMNAEWVPEERRLSTYGKG
                     RGIGDCGISQDYAWDGARFLLVEQHQMDECRGSLDWITTWRAEVRPR"
     gene            complement(7232..8515)
                     /locus_tag="VF589_04330"
     CDS             complement(7232..8515)
                     /locus_tag="VF589_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8256836.1"
                     /translation="MPKLPPAALLLAAAASAPAAAETVVVTADRMIDVLTGRVVERPV
                     VVITDGRIASVVGEGTSRPVIPSGARRIDLPGKTILPGLIDMHVHLDSNPRYGGYTGL
                     QFTDQFWTAQGVGNARAIVEAGFTTVRNVGSDNYSDVAYKQAIDEGLMQGPRIVPAAH
                     ALGATGGHCDQTYLPPSFAARGKAVGDGPQELRSRVREQRKYGAEVIKVCATGGVFSR
                     NTEPGQQQLSEEELRAIADEAHQWGLKTAAHAHGAAGIKAAIRAGIDTIEHASLIDAE
                     GIKLARARGTWLAMDIYNTEYTQAEGRKNGVLEDNLRKDREIAQIQRDGFRAAHRAGA
                     KMVFASDAGVMPHGDVGRQFRVMVEYGMTPMEAIQAATRNAAQALGRERDVGAIATGR
                     YGDIVAVDGDPIADIRQLEDVDVVIKGGAIVKSEQ"
     gene            8684..9079
                     /gene="sdhC"
                     /locus_tag="VF589_04335"
     CDS             8684..9079
                     /gene="sdhC"
                     /locus_tag="VF589_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017180897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX8256837.1"
                     /translation="MHRNPSRPLSPHLTVWRWGPHMLVSILHRVTGVGLSTAGALALV
                     WWLAAAASGEEAYASFADHADNWYGVVIAVGLSWALFQHTLSGLRHLVMDVGAGFELS
                     TNKFWANMTIVGSVLLTALLWFYILGVKA"
     gene            9076..9465
                     /gene="sdhD"
                     /locus_tag="VF589_04340"
     CDS             9076..9465
                     /gene="sdhD"
                     /locus_tag="VF589_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX8256838.1"
                     /translation="MRTESPIARVRGLGSARAGAHHWWLERLTSVATFILLVWLLVAL
                     LRLPDLGHDAIVEWLASPLVAVPMLLLIVATFWHLKLGLQVVIEDYVHEEGMKLFSIT
                     LLNFFTIALGATAFFSVLKIAFGGAPD"
     gene            9607..10704
                     /locus_tag="VF589_04345"
     CDS             9607..10704
                     /locus_tag="VF589_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256839.1"
                     /translation="MKRLWLIATAASAASLAMSAPAAAQSWQGINQRQAMLDQRIDEG
                     VRRGLLTQQEVATLRAEFRTLANLEADYRRSGGSLSSSEVAELDRRFDLLAQRVRFQS
                     LNAQGQGGIDQRTINQRQADLEMRIDQGIRNRALTQQEAILLRGEFRTIANLEAEYRR
                     SGANLSAAERADLDRRFDALARRVYQQKNDAQAGANGWRSINLRQAELDARMDQGVRN
                     GALTQQETYMLRGEFRRLADLEAQYRSAGGGLNAQERDDLERRFDQLSRRIYVNKQDR
                     QGAGGIWQSIDQRQSILEQRIDIGLRSGRLTQTEAVRLRSDFRLLANIEADYRRSGGG
                     LSIDERADLDRRFDVLTARIRIESNDWQRRW"
     gene            complement(10947..11177)
                     /locus_tag="VF589_04350"
     CDS             complement(10947..11177)
                     /locus_tag="VF589_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256840.1"
                     /translation="MTTTLDISLDALAEAAAEASAAAEAEARAAGIKPAGLLKRGPRT
                     APGRNGAKGGLGGRKGRQGSPLFSASTCAGTL"
     gene            11313..>12754
                     /gene="sdhA"
                     /locus_tag="VF589_04355"
     CDS             11313..>12754
                     /gene="sdhA"
                     /locus_tag="VF589_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX8256841.1"
                     /translation="MPEAYKIIDHSYDVVVVGAGGAGLRATMGSAETGLKTACITKVF
                     PTRSHTVAAQGGIAASLGNMGADHWTWHMYDTVKGSDWLGDQDAIEYLCREAPAAVYE
                     LEHYGVPFSRTADGRIYQRAFGGMTQNMGQGPAAQRTAAAADRTGHAMLHALYQQSLK
                     YDADFFVEYFAIDLIMEAGECRGVVALCMEDGSIHRFRCQAVVLATGGYGRAYFTATS
                     AHTCTGDGNAMVLRAGLPLQDMEFVQFHPTGIYGAGVLITEGARGEGGYLTNSEGERF
                     MERYAPSAKDLASRDVVSRSMAMEIREGRGVGKEKDHIYLHLDHIDAKILAERLPGIT
                     ETGKVFAGVDLTRQPLPVAPTVHYTMGGIPTNYHGEVVTLKDGNPDAVVPGLFAIGEA
                     ACVSVHGANRLGSNSLIDLVVFGRATGLRVRDILKPGTPHNPLPKGSEELSLSRLDKF
                     RHASGGSPTAQIRGEMQRTMQKHCAVFRDNA"
BASE COUNT         1954 a         4329 c         4451 g         2020 t
ORIGIN      
        1 tataaggaat gagatgagga gcaatgcgat gaagccgatc catatggcgt ttggcgcggc
       61 cgcgactttg gtcgcggcgc tggcgcttgg cggctggtcc ccggccgcgg cgcaggcgca
      121 gggcagccgc atcaagggca agaccccgca gaattttccg tacgagatcc gcaacgggcg
      181 gcgcgtgccc aaggcgcagc gcgtcgttgc ggcggacggg tcgtggcgcg aggaagtgcg
      241 cgacggaccg tgcatgacga tacgcgagcg cactgcccaa ggcgaatatc gcgaagtcag
      301 aaagtgcgac tgagcgaggc cggctgaagg cacaaagcgg cggccgcgtc gttccggctc
      361 gatgggcccg atcgatggga gcgcggcgcc gatgcacatg atccggacgg gtgaaggacc
      421 caagctgctg ctcgtccacg ggctcggcag cagcgccgcg tcctgggcgc cgatcgcgcc
      481 ggcattggcg caagggcgcg agctgatcgc gatcgacctg cccggccatg gcgagactcc
      541 ggcggaaggc gacagcggca ctttcaccgg gctggcgcgc agcctcgaag cattcctgga
      601 cgagcagggg ctgaccggtg tcgacgccgt cggcctctcg ctgggcggcc ggctggtgct
      661 ggaaatggcg cggcgcgggc gggtcggagc gattgtggcg ctcgatccgg gcggcttctg
      721 ggaaggctgg gagcggagct atttgcgggt gacgctgggc gcttcggtgt ggctcgtgcg
      781 ccggctggga gcggcgcttc cggccctcgc ggcggctccg ccgtcgcggg cgatgctgct
      841 tgcccaattt tccaaccggc cgtgggacgt gcccgccggc gaggcggcgc gcgagctcgg
      901 cacaatcgcc gcgacgccga ctttcgatgc attgctcgac gacctcgcct tcgggccggt
      961 gcaggccggg ccggcggcgg cgggggcggg gccggtgacg atcggctggg gccgcgacga
     1021 ccggctctgc ctgccggtcc aggcgctgcg ggcgcaggag gcgttcccgt cggcgcagct
     1081 gcactggttc gacgattgcg gccatttcgc catctgggac aagccgcggc aagtgatcga
     1141 gctgatcagg aacagcaccg gttaaacgaa ccggccatag aggtgaacga ggtgtaatgg
     1201 ccggttcacg caacccgctc cgcaattggc cgttatgagg tgagaccctc gtcgaggggg
     1261 agatttggga gtatattatg aagagcatca ttttggccgc gacggtggcg gcgatcggcc
     1321 tgccagcggt ctccgccgcg gcgcagacgc cgagccagac caatcgcgaa tatcagcgcg
     1381 acgtgaacga cgcgcgccgc gattatcagc gcgacatgcg cgacgccgac aatcgcggcg
     1441 agcggcgcga ggcgcgcgag gaatatcgcg aggagatgcg cgacgcgcgc caggacaggc
     1501 gcgaggatct gcgcgactgg cgccggtacc gcagctacga ttacaaccgg ctcgagcccg
     1561 gtcagcagcg ttattatgcc gaccgctatt atcgcagcgg caactactac cagccgcggc
     1621 ggctcggcca tggcgagcgc atctatcgcg gcaatgacgg gcgctattat tgccgccgcg
     1681 acgacggcac caccggcctg atcgtcggcg ggctggccgg cggcgtgctg ggggccgagg
     1741 tcgccccggg cgacagccag gtgctgggca cgatcctagg cggcgccgcg ggcgccctgc
     1801 tgggccgcgc cctcgaccgc gaggtcacct gccactaaag gtggcgtaat cgtcagaaaa
     1861 gggtccggcg gcacgtccgc cgggcccttt ttcatgcccc ttcgaagcgg caggcgggga
     1921 ggcccagctg agcggtttgc gtgcagcttc gcacctgcac gtcgccattg ggcgcgatga
     1981 acaaggacca gtcgttgccg tccgagcagc gcaccgtcca catcgacatg ttcttgtaat
     2041 cctcctggta gccggaccgg tcgaccttct tgcaatcctg gccggtatcc tggatcgccc
     2101 gccggagcgc gagcccgcgg ttgagttcgc tgagcgtgcg caattgctcg acataggggc
     2161 tgctcgcctt caccttggtg gtgttggccg gctcgccgcc gccgtcgcag ccggccagcg
     2221 ccagcatcag catcgcggcc aatcccgccg ccttggcccg gttcagcatg ttcgactccc
     2281 tcatccgccg taaatccgcc attcgccttg tttgtccggc cgacgcaacg tgaccgtgcc
     2341 ggcgctgctg aacggttttc cctgccgcgt cacgccatat gtctgcaccg gcacctcgac
     2401 gtaaagcgat ccgcccgctt cgaccaccgg cgagggggcg ccgatggtgg cgcgatattg
     2461 ggaatagccg gaaaaggcgc gggcgaatgc ggacgcgtcg ccgcgcttcg ccggtcgctc
     2521 gcgcagctgc caggcagcct cgtagcggcc ggcctcgatt agccggtaat aagtgcggag
     2581 cgttgcggcc gccgcctcgg cgctattgcc gccgggagct ggcgcggcca cggccggggc
     2641 gggccgggga gttgcggccg gcatcctggg ctccgaaatg gcactcccaa gctcgctctc
     2701 gggcggcggg tcggcggtcc tgctatcgcc ggagcaggcg gcgagggcga gcagcgcgat
     2761 cgacaaatgc gggagcgaag gcggcatcgc tgcgacgcta agccttcggg gccgcttcca
     2821 ccaccgcctt ggcggcttcg agcggacggt cgaggttcag cgccttggcg accgccacgg
     2881 catcgtcgaa gccggcgccg cagaaggtcg ggatgccggc ggcctcgatg ccggcgagga
     2941 gctccccctc ggggcccgag cggcagctgc cgtcgacgtc gcggatgaat acgccggcga
     3001 cgcgggagcc gaaatcctgg accacccgcg cgtagatggc gacgtcgcgc tggccgttgt
     3061 cgccgatcag caggaagcgg aagccggggt agaaagccag caccgtctcg atcgcggcga
     3121 gcttgtgcgc gtcatggctc ttgccgacca gctggtcggc gtcgatgccg taatccttga
     3181 ggaacatggg gccgtgcggg atcccgttca gctccatgaa ctcggcgatg aagccgtaga
     3241 ggttccaggg cgaggaggag acgtagaaga aggggcggac cggcgcgaca tggtcgccgg
     3301 cgatggtctt gtagaggctc gacgcgcccg gcaccgccag ccggtcctgc gggcgatcga
     3361 ccagcacccg gcgccagttc ttgacgaagt cggtggcgcc ggtttcgacc acggtatcgt
     3421 cgatgtcgga aatgatgccc caatgattgt cggcgcccgg cgccattacc ggcacctgga
     3481 ccgacgccgc ctgggcctcg cgtccgggcg tggacaaggt cacctgctcc caggcggtgc
     3541 gctcgggcag cggcagcggg accggaaggt cgaaggtgaa atagccctcc tcgtcggtga
     3601 ccgaatcgtg ggtgccgcca tagccgtcga ggcggaccgc gatgccgggc agctcatggc
     3661 tgttgtaaat ctccatcatc gcgcggagcc gggtcagcat gccctcgccg gcgtcgagcg
     3721 gcttgcggta gcggacgatg cggcccttga ggcggaggcg ctcggaattg cggtaaccgg
     3781 cataagcgag cagccggacc ttgcggtcga gcccctcgcc ggcggcggcg agatcctcga
     3841 tccggtcgat gtcgcgctcg atgtcggcgg cgagggcgtg gaggaagcgg cggaacggaa
     3901 ccatgggtcg cgaacggcaa gccgcgccgc gcggttccca aagcgcgccg ggccgcccga
     3961 accgccgacc gcgcccaagc gttgctgcgc ggcaaggaga accaaaatgg gaaacaagca
     4021 tcttcgcccc gacgagaccc cgctcgacct gatcgacgat ctcgtgcgca accccggcat
     4081 cggccagtcc aagggcgtgt tcgcgcgcgc cggcggcggc gattccgacc tcattcaggg
     4141 cgagaatacg gtcgagggcg accgcgagaa cgacaccgac ctcggcgggg ggatcgacaa
     4201 atacgacctc gaccgcaacg gcgtctagca ggctccgcac cccgtcgctg cggggctagg
     4261 ctccccgcag cgacgcccgg gcctggtcct ttacaggacg tatttcgaca ggtcggtgtt
     4321 gcgagcaacc tcggcgagct ggcggtcgac ataggcggcg tcgacctcga tcttctcgcc
     4381 gcgccggtcc tcggcctcga agctgacgtc ctcgagcagt ttctccatca ccgtctggag
     4441 gcggcgggcg ccgatattct cgatctggcc gttcacctcg gcggcgatgc gggcgatcgc
     4501 gcggatgccg tcgtcggtga agctgacgtc gaccccttcg gtggcgatga gggcgcgata
     4561 ctggtcggtg aggctggcct ccgtatcctt gaggatgtgg acgaaatcct cttcggtcag
     4621 cgccttcagc tcgacccgga tcggcagccg gccctggagc tcgggcagca ggtccgacgg
     4681 cttggcgacg tggaaggcgc cgctggcgat gaacaggata tggtcggtct tcatcgggcc
     4741 gtatttggtg gcgacggtgg tgccttcgat cagcggcagc aagtcgcgct ggacgccctc
     4801 gcggctgacc gagccgccgc gcacgtcgga cacggcgatc ttgtcgatct cgtcgaggaa
     4861 gacgatgccg ttggcctcgg cgtcggcgag cgcggcgcgg ttgacgtcgt cggtgtcgag
     4921 gcgcttgtcg gcctcctcct cgaccagccg cggccaggct tcggcgacct tcatgcggcg
     4981 gcgcttcttg ggcgcctggc cgagcgcctt ggacatcatg tcggaaaggt tgatcatgcc
     5041 gacctggccg cccatgccgg gtatctcgaa cggcgtcgcc ggggtttcgc tgacttcgat
     5101 ctcgatttcg ctggcggcga gatggccgtc gtggaagcgc tgccggaagc tggcgcgggt
     5161 cgcctcgctg gcgcccgtgc cggtgagcgc gtcgagcagc cgctccatcg ccgcttcctc
     5221 ggccgcgtcc ttgacggcga tgcggcggcg ctccttttcg agccgcaccg cttcctcgac
     5281 caggtcgcgg gcgatctgct cgacgtcgcg gccgacatag ccgacctcgg tgaacttggt
     5341 cgcctcgacc ttcacgaacg gcgcgtcggc gagcttggcc agccgccggc tgatctcggt
     5401 cttgccgcag ccggtggggc cgatcatcag gatgttcttg ggcgtcactt cctcgcgcag
     5461 cgcgtcggag agctgctggc gccgccagcg gttgcggagc gcgacggcga ccgcccgctt
     5521 ggcgtcccgc tggccgacga tatgcgcgtc gagcgcggcg accacggcct tgggggtgag
     5581 cttctcgttc atatgtcccg cggcggggga tacgccggaa aattggtaat tcaacgcgct
     5641 gtctccagct tttcgacggt gagattgtcg ttggtgtaga cgcagacttc ggcggcgatc
     5701 ttcatcgcct tgcgggcgat ggtctcggcg tccgcttcat attcgtccag cgcccgtgcg
     5761 gcggcgagtg cgaaattgcc acccgagccg atcgcggcga cgccgtcctg cggctcgagc
     5821 acgtcgccat tgccggtgag gatcaaggtc acctccttgt cggcgacgat catcatcgcc
     5881 tcgaggttcc tgagatattt gtcggtgcgc cagtccttgg cgagctcgac cgcggcgcgc
     5941 atcagggcgc catgatgctg ctccagcttg cgctcgagcc gctcgaacag ggtgaaggcg
     6001 tcggcggtgg cgccggcgaa gccgccgatc accgagccgt cgccgagccg gcggaccttg
     6061 cgggcattcg gcttgatcac cgtctggccc agcgtaacct ggccgtcgcc ggcgaccacc
     6121 gcatgcccgt tcttccgcac cgacaggatc gtcgtcccgt gccagctttc catgattgct
     6181 cccgctctag gcccgggcgt tatgtgggaa aggcgtcgaa aaggtgcaat gccaacgcgc
     6241 gaaggacggt agcccgggct tcagcgcggc cgcacctcgg cgcgccaggt ggtgatccag
     6301 tcgagcgagc cgcggcattc gtccatctgg tgctgctcga ccagcaggaa gcgggccccg
     6361 tcccaggcat aatcctgcga gatgccgcaa tcgccgatgc cgcggccctt cccataggtg
     6421 ctgagcctcc gctcttccgg cacccattcg gcgttcatca gccaattggt ctcgccgccc
     6481 ttctcgcgct cgaactcgcc gggccgggcc ttgcccgctt cgtcgaggac cagggcgacg
     6541 ctgaggaaat tgtaggctcc gctgccgcat ttgacgggca ccaggaccag gctgtggcga
     6601 tcgtcgaggc gatgggcctg ggcggcttcc tcttcggccg gggtttcgca ggtcatgtcg
     6661 tcgcgggcgg cggcgacggc ggcggccgac gccatccgcg ccggcttcga cgatgcgggc
     6721 ggcgcctgca ccaccggcag cggcggcggc ggcggcactg cgctggccgg cttgtccccg
     6781 cgccgggtca gcgccgtcac cgtgccgacc cggccctgct tgtcgtcgat gtagagcaag
     6841 gccgcgctgg agcccgctgt cgagagtgtg ccgaccggct tgccgtcggg gccgatcaaa
     6901 gccgccttct tgccggcgag caaagcggcg aggaaggcgc gggcctgcgg cgggacggcg
     6961 gtgtcgccgt cgtcgctggc cacgtcgagc gccttgaagc ggcggccgtc aacctcgacc
     7021 caaggcgcac cctcggccgc cattgagaaa gcgacgcgcg gctcagcgcc ggcttcgggg
     7081 cggcgcagca ccatcatcgt cgcggcgtcg agatcgtcct ccggcaccag cccgaccgcc
     7141 tggcagagcc ggccattgtc gcagcccacc gcccagtcgc tgaaggtctt cagcggcgcc
     7201 ggctcggcgg cggcgagcag caaggcgagc atcactgctc ggatttgacg atggcgccgc
     7261 ccttgatcac gacgtcgacg tcctcgagct ggcggatatc ggcgatcgga tcgccatcga
     7321 ccgcgacgat gtcgccatag cgtccggtcg cgatcgcgcc gacgtcgcgc tcgcggccca
     7381 gcgcctgcgc ggcgttgcgg gtcgccgcct ggatcgcctc catcggggtc atcccatatt
     7441 cgaccatcac ccggaattgc cggccgacat cgccgtgcgg catcaccccg gcgtcggaag
     7501 cgaacaccat cttggcgccg gcgcggtggg cggcgcggaa gccgtcgcgc tggatctgcg
     7561 cgatctcgcg gtccttgcgg agattgtcct caaggacgcc gttcttgcgg ccctcggcct
     7621 gggtatattc ggtgttgtaa atgtccatgg cgagccaggt gccgcgggcc cgcgccagct
     7681 tgatgccctc ggcgtcgatc aggctggcat gctcgatcgt gtcgatcccg gcgcggatcg
     7741 cggccttgat gccggccgcg ccatgggcgt gggcggcggt cttcagcccc cattgatggg
     7801 cttcgtcggc gatcgcgcgc aattcctcct cggaaagctg ctgctggccg ggctcggtgt
     7861 tgcgcgagaa gacgccgccg gtggcgcaga ccttgatcac ttcggcgcca tatttgcgct
     7921 gctcgcggac gcggctgcgg agctcctgcg gcccgtcgcc gaccgccttg ccgcgcgccg
     7981 cgaaggaggg cggcaaatag gtctggtcgc aatggccgcc ggtggcgccg agcgcatgcg
     8041 cggccggaac gatgcgcggc ccctgcatca gcccctcgtc gatcgcctgc ttgtaggcga
     8101 cgtcggaata attgtcggag ccgacgttgc ggacggtggt gaagcccgcc tccacgatcg
     8161 cccgcgcatt gccgacgccc tgcgcggtcc agaactgatc ggtgaactgg agcccggtat
     8221 agccgccgta gcgggggttg gaatcgaggt gcacgtgcat gtcgatgagg ccgggcagga
     8281 tggtcttgcc gggaagatcg atccgccgcg cgcccgacgg gatgaccggc cgcgacgtgc
     8341 cttcgccgac taccgaggcg atgcggccgt cggtgatcac caccaccggc cgttcgacga
     8401 cgcggccggt gagcacgtcg atcatgcggt cggcggtgac gacgacggtc tcggccgcgg
     8461 ccggcgcgga agcggcggcg gcgaggagca gggcagcggg cggcaatttc ggcatggacg
     8521 gctccgtata aggtgtcccg ccgtcctagc cgggcgcggc gggcggtgtc gagccggctg
     8581 cggcaccgac gccgcccttc gcaaggtgca cacccgccca cccttgcatc cccctgccca
     8641 aggcctaaaa gcgcccccgt ccaagaggga gccttccggc ctcatgcacc gcaacccgtc
     8701 ccgtccgctc tcgccccatt tgaccgtctg gcgctggggc ccgcatatgc tggtctcgat
     8761 cctccaccgc gtcaccggcg tcggcctgtc gaccgccggc gcgctcgccc tggtctggtg
     8821 gctggcggcg gcggcgagcg gggaggaagc ctatgccagc ttcgccgacc atgccgacaa
     8881 ttggtacggg gtggtgatcg cagtcgggct cagctgggcg ctgttccagc acaccctttc
     8941 gggcctccgg cacctcgtca tggacgtcgg cgcgggcttc gagctttcga ccaacaaatt
     9001 ctgggccaat atgacgatcg tcggctcggt cctgctcacc gctttgctgt ggttctacat
     9061 attgggagtg aaggcatgag gacggagagc ccgatcgccc gggtccgcgg cctcggctcg
     9121 gcccgcgccg gcgcccatca ttggtggctg gagcggctga cctcggtcgc gaccttcatc
     9181 ctcttggtgt ggctgctggt cgcgctgctg cggcttcccg acctcggcca tgacgcgatc
     9241 gtcgaatggc tggcttcgcc gctggtggcg gtgccgatgc tgctgctgat cgtcgccacc
     9301 ttctggcacc tgaagctcgg gctccaggtg gtgatcgagg attatgtcca tgaggagggc
     9361 atgaagctgt tttcgatcac cctgttgaac ttcttcacca tcgcgctcgg cgcgacggcg
     9421 tttttctcgg tgctcaagat cgcgttcggc ggcgcgccgg actgagtgcg gcgatgtcgg
     9481 acgggcgtgg cgacgctccc gaagatgaca gccagactaa cggaccggcc atgaaacgtt
     9541 cagccggcac ggtctaatcc cgcaacatcc gccgtaaccc ggcgcaagta atggaaaagg
     9601 tgcagcatga aacgtttatg gcttatcgca accgcggcct ccgccgcctc gctggcaatg
     9661 agcgcgccgg ccgccgccca gagctggcag gggatcaacc agcgccaggc gatgctcgac
     9721 cagcgcatcg acgagggcgt gcgcaggggc ctgctgactc agcaggaagt ggcgacgctg
     9781 cgggccgaat tccgcacgct ggccaacctc gaggccgatt atcgccgctc cggcggcagc
     9841 ctgtcgtcaa gcgaggtcgc cgagctcgac cggcgcttcg acctgcttgc ccagcgcgtc
     9901 cgcttccaga gccttaatgc gcagggccaa ggcggcatcg accagcgcac catcaaccag
     9961 cgtcaggccg acctcgagat gcggatcgac caaggcatcc gcaatcgcgc gctgacccag
    10021 caggaagcga tcctgctgcg cggcgaattc cgcaccatcg ccaatctcga ggcggaatat
    10081 cgccgctcgg gcgccaatct gtcggccgcc gagcgcgccg atctcgaccg gcgtttcgat
    10141 gcgctcgccc gccgcgtcta ccagcagaag aacgacgccc aggcgggcgc gaatggctgg
    10201 cggtcgatca acctgcgcca ggccgagctc gacgcccgca tggaccaggg cgtccgcaac
    10261 ggcgcgctga cccagcagga gacctatatg ctccgcggcg agttccggcg gctcgccgac
    10321 ctcgaggcgc aatatcgcag cgccggtggc ggcctcaacg cccaggagcg cgacgatctc
    10381 gagcgccgct tcgaccagct gagccggcgg atctatgtca acaagcagga ccggcagggc
    10441 gccggcggca tctggcagtc gatcgatcag cgccagtcga tcctggagca gcgcatcgac
    10501 atcggcctgc gcagcggccg gctcacccag acggaggcgg tccggctgcg gagcgacttc
    10561 cgcctgctcg ccaacatcga ggccgattac cggcgttcgg gaggcggcct cagcatcgac
    10621 gagcgcgccg acctcgaccg ccgcttcgac gtgctgaccg cccgcatccg gatcgaatcg
    10681 aacgactggc agcgccgctg gtaagcaacg gctagtccga cggccattct ctctcccggc
    10741 tccgggagag ggaatggacc caggtccctg tggagcgacg caaggccgtc gcaccgaatt
    10801 gcgccccccc agcgtcccac cagccttgca ggacctagaa acgcccgggc cgacgccgca
    10861 ggcgggacga gctcagggcg acaacaggcg ccgtcgcagt aattgcccaa ccgggtcttg
    10921 tgcccagcgc ggcaggctag ccacggttac agtgtgcccg cgcaggtcga ggcactgaaa
    10981 agcgggctcc cctgcctgcc tttccggccg cccagcccgc ccttcgcccc attgcggccc
    11041 ggtgccgtgc gcggcccccg cttcaacagg cccgccggct tgatgcccgc ggcgcgcgcc
    11101 tcggcctcgg cggccgccga ggcttctgcg gcagcctcgg caagagcatc gagggaaatg
    11161 tcgagcgtgg tagtcacatg ccctttctat cacggcggcc ggcatccagg caaacgccgg
    11221 agacgaggtg gcgatccgcc gccctaccct tgcaacctcg ctcccggggc ctaaaagcgc
    11281 ccgcgaaggt ccccctcgcg cgagccccag atatgcccga agcctataag atcatcgatc
    11341 atagctacga cgtggtcgtc gtcggcgccg gcggagcggg gctgcgggcg acgatgggat
    11401 cggccgagac gggcctgaaa accgcctgca tcacgaaggt gttcccgacc cgcagccaca
    11461 ccgtggccgc gcagggcggg atcgcggcca gcctcggcaa tatgggcgcc gaccattgga
    11521 cctggcacat gtacgacacc gtcaaagggt cggactggct cggcgaccag gacgcgatcg
    11581 aatatctgtg ccgcgaggcg ccggccgcgg tctacgagct cgaacattat ggcgtgccgt
    11641 tcagccgcac cgccgacggg cgcatctacc agcgagcgtt cggcggcatg acccagaata
    11701 tgggccaggg cccggccgcc cagcgcaccg ccgccgccgc cgaccggacc gggcacgcga
    11761 tgctccacgc gctctaccag cagagcctga aatacgacgc cgacttcttc gtcgaatatt
    11821 tcgcgatcga cctgatcatg gaagccggcg aatgccgcgg cgtcgtggcg ctgtgcatgg
    11881 aggatggcag catccatcgc ttccgctgcc aggcggtggt gctcgccacc ggcggctacg
    11941 ggcgcgccta tttcaccgcc acctccgccc atacctgcac cggcgacggc aatgccatgg
    12001 tgctgcgcgc cggcctgccg ctccaggaca tggagttcgt ccagttccac ccgaccggca
    12061 tttacggcgc cggagtgctg atcaccgagg gcgctcgagg ggaagggggg tatctcacca
    12121 actcagaggg cgagcgcttc atggagcgct acgcgccgtc ggccaaggat ttggcgtcgc
    12181 gcgacgtggt ctcgcgctcg atggcgatgg agatccggga gggccgcggg gtcggcaagg
    12241 agaaggacca tatctacctt cacctcgacc atatcgacgc gaagatcctg gccgagcgcc
    12301 tgcccggcat caccgagacc ggcaaggtgt tcgccggcgt cgacctcacc cgccagccgc
    12361 tgccggtggc gccgaccgtc cattatacga tgggcggcat cccgaccaat tatcatggcg
    12421 aggtggtgac gctgaaggac ggcaatcccg acgcggtcgt gccgggcctg ttcgcgatcg
    12481 gcgaggcggc ctgcgtctcg gtccacggcg ccaaccggct cggctccaac agcctgatcg
    12541 acctggtcgt gttcggccgc gccaccggcc tgcgggtgcg cgacatcctg aagcccggca
    12601 ccccgcacaa cccgctgccc aaggggtcgg aagagctgtc gctgtcccgg ctcgacaagt
    12661 tccgccacgc cagcggcggc tcccccaccg cgcagatccg cggcgagatg cagcgcacga
    12721 tgcagaagca ctgcgccgtg ttccgcgaca acgc
//
LOCUS       DASVRK010000117        10132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_207348, whole genome shotgun sequence.
ACCESSION   DASVRK010000117 DASVRK010000000
VERSION     DASVRK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207348"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VF589_04360"
     CDS             complement(<1..1008)
                     /locus_tag="VF589_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256842.1"
                     /translation="MAIKTGTNVNDRLNGTYDDDSIYGLLGNDIINGGYGSDYLYGDR
                     EYSWSSPADGGADTISGGFNSDYIYGDAGNDTLNGDSGDDSLYGGVGRDALNGGTGND
                     WLYIGNVTEMSGDRVDGGSGVDVLIGDYSARATRLVFTALDARTAASFGNLSVINVER
                     YHLNATAFNDALTGWRHDDEFTAGAGNDTLNGMAGADTLDGEDGSDKLYGGSGDDDLT
                     GDEYGQTGVDYLDGGFGDDDLDGGGGNDTLVGGAGDGNDDLDGDDGDDKLNGGAGNDS
                     LYGGRGVDSIIGGSGDDSISSDSYWYVTYSGDTGTEKDTIDAGTGNDDVLIGIDDSAK
                     GG"
     gene            complement(1215..1289)
                     /locus_tag="VF589_04365"
     tRNA            complement(1215..1289)
                     /locus_tag="VF589_04365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1255..1257),aa:Gly,seq:gcc)
     gene            1457..2368
                     /locus_tag="VF589_04370"
     CDS             1457..2368
                     /locus_tag="VF589_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8256843.1"
                     /translation="MATLGMSAAERDAIEAFRTDVIEPSMTALVILDFWAEWCGPCKQ
                     LTPLLEKVTADYAAKGVRLAKINVDENRAIAAQFRVQSIPTVYAVFQGQLVADLTPAR
                     TEAQLTRFLDQILAQLPIEGEAQQAEAEIAPLVEMGEQVLAEGDAERAANIFAQLLDM
                     APDQPDVISGLARALAAAGRTEEARQLLDAAPEAAAGDPAIARARAALALAEAAQPDA
                     DTAAFERRIAADPDDHEARYELAGVLMSRGERDAAADQLFEIIARDRGWNEGAARQRL
                     LQLLEVVGIADPWASQQRRRLSAILFT"
     gene            2368..2997
                     /locus_tag="VF589_04375"
     CDS             2368..2997
                     /locus_tag="VF589_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX8256844.1"
                     /translation="MPGRLRIPIFPLPGALLFPRSQLPLHIFEPRYRAMLRDALAGDR
                     MIGMVQPRDEREPPALFAVGCLGRIVACEELPDGRFNIVLEGVARFRLAAEVDAATPY
                     RQVEADLNGFEDEAEPEPLASIQRAELERESRRYADALGYSVDWEAVGKLDDETLVNG
                     IAQIAPLDTGSKQALLEADGLAARADLLVQFMEFQRMIPGGADGVETLQ"
     gene            3003..3167
                     /locus_tag="VF589_04380"
     CDS             3003..3167
                     /locus_tag="VF589_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX8256845.1"
                     /translation="MFDERLLAKLVCPATRTPLRYDAAAEELVSDEAGLAYPVRAGVP
                     VLLIEEARRL"
     gene            complement(3184..4392)
                     /locus_tag="VF589_04385"
     CDS             complement(3184..4392)
                     /locus_tag="VF589_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEX8256846.1"
                     /translation="MERADVLILGGGLVGLTLAIALGKHGVATIVVDPADPERTLTPQ
                     YDGRATAVASASWRMLEAIGVGERLEGQGCPIEAIRVSDGLDPGGLVFDPPAGDDPLG
                     IMFENRLLRAALRDTAMAAPNVSLVMPARPAEVVRDANGARVALEDSRLLTASLLIGA
                     EGRSSPTREAAGIPIARWSYDHVAIVATIEHERPHENVAYEIFYPSGPFAILPMLPGT
                     RSAIVWSVSAGDAAATIDLPERALAAEIEKRMGGFLGTVHMAGPRASYPLGFHHAATI
                     TGQRLALVGDAAHGIHPIAGQGLNLGFRDAAALAQVLVEGARLGLDYGDAQLLARYER
                     WRSLDTLMVAAATDGLTRLYGIPGRTASAVRRFGMSAVQRIRPFKDQLMAEARGESGD
                     LPLLLRGLAI"
     gene            4491..6827
                     /locus_tag="VF589_04390"
     CDS             4491..6827
                     /locus_tag="VF589_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX8256847.1"
                     /translation="MATRAAGGGRQQRLAQWREGVRRAVRRSGSIAGGAGLIAVTLLL
                     LLALVSYRPTDPAFNTAAGGPARNWMGDAGAYAADLLLSLWGPAAGLLLPLAVVLGLR
                     LARGAGVGRWLRALLLSILGIVFLGTSVALLFGGAVNGLPAGWGGTFGLSLAALVELG
                     IGAIGEPGIVDPFRVVSVALIALAGLFLWYLGLGLRAEERQWLTVRRSAARPGSAAKP
                     LRDLKSIEPPEPVQRPARAPVITPPEPPRTIIADRVKTPDTGRRPQRERQPSLALGDA
                     YALPTLDLLNPPPPPVNTTLDKASLERNARLLESVLEDFSVKGEIVEVRPGPVVTMYE
                     LEPASGIKASRVIQLADDIARNMSALSARVATIPGRSVIGIELPNAKREMVTLSELIA
                     SHAFEDQVASLPLILGKNIAGDPVIADLAPMPHLLVAGTTGSGKSVGLNCMILSLLYR
                     LTPEQCRMIMIDPKMLELSIYDDIPHLLAPVVTEPAKAIRALKWTVEQMEERYRMMAS
                     VGVRQLSSFNAKVREAKAKGQPLGRRVQTGYDQETGQPKYEMEELEYDVLPQIVVVVD
                     ELADLMMTAGKEVEFLIQRLAQKARAAGIHLIMATQRPSVDVITGVIKANLPTRISFS
                     VTSKIDSRTILGEQGAEQLLGKGDMLYMPGGKQIARVHGPFVTDEEVRAVADFWRTQG
                     HPEYVQSVTEEPEDGGYMFEGQPTGEDDPDSQLYRKAVQTVAESQKASTSYIQRQLRI
                     GYNNAARLIERMEKDGIVSAPDHVGRREVLVDAEGHAI"
     gene            6917..7540
                     /locus_tag="VF589_04395"
     CDS             6917..7540
                     /locus_tag="VF589_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX8256848.1"
                     /translation="MLRSMTLALAAVPLTVALAPQVAAAPASAPLAQVQTHLRTVDTM
                     TANFAQTDRRGKTLTGTLLLKRPGRVRFQYQKGVPLLVVGDGNALTMIDYEVKQVSRW
                     PIGNSPLSVLLNPSKDISKVAKVVREDAKVLLVQARDRSRPEFGTITIAFAKVPSAPA
                     GLILQGWSTLDAQNNRSTIKLSNQRFNVAVADTAFRWTDPRKRGPRG"
     gene            7939..9495
                     /locus_tag="VF589_04400"
     CDS             7939..9495
                     /locus_tag="VF589_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256849.1"
                     /translation="MPYFVFGQERDWDGYGQDTFVVQIEDPDRVAHARGLIAGTETDR
                     PLIGGVVTMAPADYNIGWSFHIAPPDLVFADGFTEIGDNDIRGVEAGVSFFGDGFLPG
                     NRWTPWTVYIVTELNLVSGGGNADNLQGTQLPDIVFGRAGRDTLSGGEGDDHLIGEGG
                     MDRLQGGIGRDKLSGGDGADSLNGGAGKDILDGGLGADTIAGGTGNDTYHVRAGEDVI
                     TEAAAGGVDAIWSWISYTLAHQNVEDLRLMDSGTANIDGTGNALDNRIWGNWEINVLR
                     GREGNDTLDGHAGNDVLVGGNGNDTLIGGGGDSDTASYASAAGAVRANLALTTPQDTL
                     GAGTDTFAAMPWETMTSIENVVGSAFADRLRGNSEANSLNGGPGADRLEGGAGNDRLN
                     GGGGNDSLHGGAGNDRLTGGTGEDAFWFDAAPEASNVDRILDFSAARDTLFLDRSAFD
                     AIGADGTLAASAFHAGTAAHDASDRIVYDAATGKIFYDADGSGAGAAILFATVDTETS
                     LTHADFNIVA"
     gene            9584..>10132
                     /locus_tag="VF589_04405"
     CDS             9584..>10132
                     /locus_tag="VF589_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8256850.1"
                     /translation="MASNLKIASWNINSVRARIEIVEKFLREERPDILCLQETKVVDG
                     DFPRAAFHRLGYRHIILCGQRMHHGVAIISRIPMREDDRLDWQANGEARHCGVQLECG
                     IRLENVYVPAGGDVPDRTVNPKFGQKLDFIERMTRWSEQCRRPTLLVGDFNVAPLECD
                     VWSHKQLLGVVSHTPVEVEALTR"
BASE COUNT         1662 a         3428 c         3449 g         1593 t
ORIGIN      
        1 gccgcccttg gcgctgtcgt cgatcccgat cagcacatcg tcgttgccgg tgccggcgtc
       61 gatcgtgtcc ttttcggttc cggtgtcgcc cgaataggtg acgtaccagt aggagtcgga
      121 gctgatgcta tcgtcaccgg agccgccgat gatcgaatcg acgccgcggc cgccatagag
      181 gctgtcattg ccggcgccgc cgttgagctt gtcgtcgccg tcgtcgccgt cgaggtcgtc
      241 gttgccgtcg ccggcgccgc cgaccaaagt gtcgttgccg ccaccgccgt cgagatcgtc
      301 gtcgccgaag ccaccgtcga gatagtcgac accggtctgg ccgtattcgt cgccggtcag
      361 atcgtcgtcg ccggagccgc catagagctt gtccgagccg tcctcgccgt cgagcgtgtc
      421 cgcgccggcc atgccgttca gcgtgtcgtt gcccgccccg gcggtgaatt cgtcgtcgtg
      481 cctccagccg gtaagggcat cgttgaaggc ggtggcgttc aggtggtaac gttcgacatt
      541 gatgaccgac aggttaccga aactcgcggc cgtccgcgca tcgagggcgg tgaaaacgag
      601 gcgcgtcgcg cgggccgaat aatcgccgat cagcacgtcg acgccggacc cgccgtcgac
      661 ccggtcaccg gacatctcgg tcacgttgcc gatgtagagc cagtcattgc cggtgccgcc
      721 gttcagcgca tcgcggccga cgccgccata gagcgaatcg tcgccggaat cgccgttcag
      781 cgtatcgttg ccggcgtcac cgtagatata gtcgctgttg aagccgccgc tgatcgtgtc
      841 ggcgccgcca tcggccgggc tcgaccagga atattcgcga tctccgtaaa ggtagtcgct
      901 gccgtagccg ccgttgatga tgtcgttgcc aagcaggccg taaatcgagt cgtcgtcata
      961 agtgccgttc agcctgtcgt tgacgttggt cccagtcttg atagccatct cgcgtccccc
     1021 ccttatcccg ccgagtcgaa ctgcctcgat aaatgacttt tgcgtcggct gcggtcaata
     1081 caaaaacaag ggataagtgc ttgaaccgca atgagacaaa cctgcacttt cgcgcgacga
     1141 aaatgcatag gggccggctt gcccagtcga gcataaggag ccgcgactgg ctttggacct
     1201 gtgatgtcgt gaaatggagc gggcgaaggg attcgaaccc tcgaccccaa ccttggcaag
     1261 gttgtgctct acccctgagc tacgcccgct cggcggatcg ctccgccagc ggcgggccat
     1321 caaggcggcg gcggttagca tggggtccgg gcgctcgcaa ccccctagaa gcgccccgcc
     1381 ctaatcgggg acgcgggctt ggcagactcg cacccgatcc cacatagggg gccaaaccgg
     1441 gctggagaga caaggcatgg cgacattggg catgagtgcc gccgaacggg acgcgatcga
     1501 agcgttccgg acggacgtga tcgagccgtc gatgaccgcg ctcgtcatcc tcgacttctg
     1561 ggccgaatgg tgcgggccgt gcaagcagct gaccccgctg ctggagaagg tcaccgccga
     1621 ttatgccgcc aagggcgtcc ggctggccaa gatcaacgtc gacgagaatc gcgcgatcgc
     1681 cgcccaattc cgggtccagt cgatccccac cgtctatgcg gtcttccagg ggcagctggt
     1741 cgccgacctc acgcccgccc ggaccgaggc ccagctcaca cgcttcctcg accagatcct
     1801 ggcccagctc ccgatcgaag gcgaggcgca gcaggccgag gcggagatcg cgccgctggt
     1861 cgagatgggc gaacaggtgc tcgccgaggg cgacgcggag cgggccgcga acatcttcgc
     1921 ccagctgctc gacatggcgc ccgaccagcc agacgtgatt tccggcctcg cccgcgcgct
     1981 ggccgccgcc ggccggaccg aggaggcgcg gcaactgctc gacgcggccc cggaggctgc
     2041 ggccggcgac cccgccatcg cccgcgcccg cgcggcgctc gcgctcgccg aggcggccca
     2101 gcccgacgcc gacaccgccg ccttcgagcg caggatcgcc gccgacccgg acgaccacga
     2161 ggcccggtac gagctggccg gcgtgctgat gagccgcggc gagcgcgacg ctgccgccga
     2221 ccagttgttc gagatcatcg cccgcgaccg cggctggaac gagggcgcgg cgcggcagcg
     2281 gctgctgcaa ttgctcgaag tagtcggaat cgccgacccg tgggcgtcgc agcagcgccg
     2341 gcgcctctcc gcgatcctgt tcacctgatg cccggacggc tgcggatccc gatcttcccg
     2401 ctgccgggag ccttgctgtt tccgcgctcg caattgccgc tccacatctt cgagccgcgc
     2461 taccgcgcca tgctccgcga cgcgctcgcc ggcgaccgca tgatcggcat ggtccagccg
     2521 cgcgacgagc gcgagccgcc cgctttgttc gcggtcggct gcctcggccg gatcgtcgct
     2581 tgcgaggagc tgcccgacgg ccgcttcaac atcgtcctcg aaggcgtcgc ccgcttccgg
     2641 ctggcggccg aggtcgacgc cgcgacgccc tatcgccagg tcgaggccga tctcaacggc
     2701 ttcgaggacg aggccgagcc ggagccgctc gccagcatcc agcgcgccga gctcgagcgc
     2761 gaatcgcgcc gctacgccga cgcgctcggc tattcggtgg attgggaggc ggtcggcaag
     2821 ctcgacgacg agaccctggt caacggcatc gcccagatcg cgccgctcga caccggctcc
     2881 aagcaggccc tgctcgaagc cgacggactg gccgcgcgcg ccgacctgct cgtccagttc
     2941 atggaatttc agcgtatgat cccgggcggc gccgacgggg tggagacgct ccagtgaagc
     3001 gggtgttcga cgagcgcctg ctggcgaagc tggtctgccc agccacccgg acgccgctgc
     3061 gctacgatgc cgccgccgag gagctggttt cggacgaggc gggcctcgct tatccggtgc
     3121 gggcaggagt cccggtcctg ctgatcgagg aagccagacg cctttgaggc gtgcgggcaa
     3181 ggcctagatg gcgagcccgc gtagcagcag cggcaggtca ccgctctcgc cccgcgcctc
     3241 ggccatcagc tggtccttga acgggcggat ccgctgcacc gcgctcattc cgaagcggcg
     3301 gaccgccgag gcggtgcggc cgggaatccc atagaggcgg gtgaggccgt cggtcgccgc
     3361 cgccaccatc aaggtatcga ggctgcgcca gcgctcgtag cgggcgagca gctgggcgtc
     3421 gccataatcg agcccgagcc gcgcgccctc gaccagcacc tgggcgagcg cggcggcgtc
     3481 gcggaagccg aggttgagcc cctggccggc gatcgggtgg atgccgtgcg cggcgtcgcc
     3541 taccaaagcg agccgctggc cggtgatggt cgcggcatgg tggaagccga gcggatagct
     3601 ggcgcgcggc ccggccatgt ggacagtgcc gaggaagccg cccatccgtt tctcgatctc
     3661 ggccgccagc gcgcgctcgg gcaggtcgat ggtggcggcg gcgtcgccgg ccgacaccga
     3721 ccagacgatc gccgagcgcg tcccgggcag catcggcagg atcgcgaacg ggcccgacgg
     3781 atagaagatt tcataagcga cgttttcgtg cggccgctca tgctcgatcg tggcgacgat
     3841 cgcgacatgg tcgtagctcc agcgggcgat cgggatgccg gcggcttcgc gggtcgggga
     3901 cgagcggcct tcggcgccga tcagcagcga ggcggtgagc agccggctgt cctcgagcgc
     3961 gacgcgggcg ccgttagcgt cgcgcaccac ttcggccggc cgggccggca tgacgaggct
     4021 gacattgggg gccgccatgg cggtgtcgcg cagcgccgcg cggagcagcc ggttctcgaa
     4081 catgatgccg agcggatcgt cgccggccgg gggatcgaag acgaggccgc ccggatcgag
     4141 cccgtcgctg acccggatcg cctcgatcgg gcagccctgg ccttcgagtc gctcgccgac
     4201 gccgatcgcc tccagcatgc gccacgaggc gctcgccacc gcggtcgcgc gcccgtcata
     4261 ttgcggcgtc agcgtccgtt cgggatcggc cggatcgacg acgatggtgg cgacgccgtg
     4321 cttgccgagc gcgatggcca gggtgaggcc gacgaggccg ccgccgagga tgaggacgtc
     4381 tgctcgctcc attttcccgc cctagcgatg caaagctgca acgccaagcg cttgaccgcg
     4441 gactcgccaa gggcgcatta agcatgacgg aacatttggg gcacggggtc atggcaacac
     4501 gcgcggccgg cggcggccgc cagcaacggc tggcgcagtg gcgggaaggg gtccggcggg
     4561 cggtgcggcg ctcgggctcg atcgccgggg gcgcgggcct gatcgccgtc accttgctgc
     4621 tgctgctggc cctggtcagc taccggccga ccgacccggc cttcaatact gccgccggcg
     4681 ggccggcgcg caactggatg ggcgatgccg gcgcctatgc ggccgatctg ctgctcagcc
     4741 tgtggggccc ggccgcggga ctgctgctgc cgctcgccgt cgtgctcggg ctgaggctgg
     4801 cgcgcggcgc gggcgtcggg cgctggctgc gcgcgctgct gctcagcatc ctcggcatcg
     4861 tcttcctcgg cacctcggtg gccttgctgt tcggcggcgc ggtcaacggc cttccggccg
     4921 gatggggcgg cacgttcggc ctgtcgctcg ccgcgctggt cgagctgggc atcggcgcga
     4981 tcggcgagcc cggtatcgtt gatccgttcc gggtggtgag cgtggcgctg atcgcgctgg
     5041 cgggcctgtt cctctggtat ctagggctcg gtctccgcgc cgaggagcgg cagtggctga
     5101 cggtgcggcg gtcggcggcg cggccgggtt cggcagcgaa gccgctccgc gacctcaagt
     5161 ccatcgagcc tccggagccg gtccagcgcc ccgcccgcgc gcccgtcatc accccgcccg
     5221 agccgccgcg gacgatcatc gccgaccgcg tcaagacgcc cgataccggc cggcggccgc
     5281 agcgcgagcg ccagccttcg ctggcgctgg gcgacgctta tgctttgccg acgctggacc
     5341 tcctgaaccc gccgccgccg ccagtgaaca ccacgctcga caaagccagc ctggagcgca
     5401 atgcgcgcct gctcgaatcg gtgctcgagg acttctcggt aaagggcgag atcgtcgagg
     5461 ttcgccccgg cccggtggtc accatgtacg agctcgagcc cgccagcggg atcaaggcga
     5521 gccgcgtcat ccagctcgcc gacgacatcg cccgcaacat gtcggctttg tcggcgcggg
     5581 tcgcgaccat tccgggccgc agcgtgatcg gcatcgagct ccccaacgcc aagcgcgaga
     5641 tggtgacttt gtcggagctg atcgccagcc acgccttcga ggaccaggtc gcctcgctgc
     5701 cgctgatcct cggcaagaac atcgccggcg acccggtcat cgccgatctg gcgccgatgc
     5761 cgcatctgct cgtcgccggc accaccggct cgggcaagtc ggtcggcctc aactgcatga
     5821 tcctgtctct gctctatcgg ctgacgcccg agcagtgccg gatgatcatg atcgacccga
     5881 agatgctgga gctcagcatc tatgacgata tcccgcatct gctggcgccg gtggtcactg
     5941 agcccgccaa ggcgatccgc gcgctcaaat ggaccgtcga gcagatggag gaacgctacc
     6001 ggatgatggc gtcggtcggc gtgcgccagc tgtccagctt caacgccaag gtccgtgaag
     6061 ccaaggccaa gggccagccg ctcgggcgcc gcgtccagac cggctacgac caggaaaccg
     6121 gccagcccaa atatgagatg gaggagctcg aatatgacgt gctcccgcag atcgtggtgg
     6181 tggtggacga acttgccgac ctgatgatga cggccggcaa ggaggtcgag ttcctgatcc
     6241 agcggctggc gcaaaaggcg cgcgccgccg gcatccacct catcatggcg acgcagcgcc
     6301 cctcggtcga cgtcatcacc ggcgtcatca aggcgaacct gccgacccgg atcagcttct
     6361 cggtcacgtc caagatcgat tcgcgcacca tcctcggcga gcagggcgcc gagcagctgc
     6421 tcggcaaggg cgacatgctc tacatgccgg gcggcaagca gatcgcccgc gtccacgggc
     6481 cgttcgtcac cgacgaggag gtccgcgcgg tcgccgactt ctggcgcacc cagggccatc
     6541 cggaatatgt ccagtcggtc accgaggaac ccgaggacgg cggctatatg ttcgaaggcc
     6601 agccgacggg ggaggacgat cccgacagcc agctctaccg caaggcggtg cagacggtgg
     6661 cggaaagcca gaaggcctcg accagctaca tccagcgcca actcaggatc ggctacaaca
     6721 atgccgcgcg cctgatcgag cggatggaga aagacggcat cgtctccgct cccgaccatg
     6781 tcggccgccg cgaagtgctg gtcgacgccg aggggcacgc gatctgatcg ccagtacctg
     6841 gggaacggag gagttcactg ggcattcaat cgcccgaccc catatcgccc cccgaatgaa
     6901 gcttacggag acatccatgt tgcgttccat gacgctcgcc cttgcggccg tgccgctgac
     6961 ggtcgcgctc gcgccgcagg tcgcggcggc gccggcgtcc gcgccgctcg cgcaggtcca
     7021 gacccatctt cgcacggtcg acaccatgac cgccaatttc gcgcagaccg accggcgcgg
     7081 gaagacgctg accggcaccc tcctcctgaa gcggcccggg cgggtgcgtt tccaatatca
     7141 gaagggcgtg ccgctgctgg tcgtcggcga cggcaacgcg ctcaccatga tcgattatga
     7201 agtgaagcag gtgtcgcgct ggccgatcgg caactcgccg ctgtcggtgc tgctcaaccc
     7261 gagcaaggac atcagcaagg tcgccaaggt cgtgcgcgag gacgccaagg tgctgctggt
     7321 ccaagcccgc gaccgcagcc gtcccgaatt cgggaccatc acgattgcct tcgccaaggt
     7381 gccgagcgcg cccgccgggc tgatcctgca gggctggtcg acgctcgacg cgcagaacaa
     7441 ccgctcgacc atcaagctgt cgaaccagcg cttcaatgtc gccgtggcgg acaccgcctt
     7501 tcgctggacc gacccccgca agcgagggcc gaggggctga aatcggctga aaagcgcggg
     7561 taaaagttaa cgccattcat cctcgcgaca ggaatcatgg tttacaggga agactgcaat
     7621 tgcgggtcgc cgcttcgggt tttccccctg ttgcccgacc ggttcccgca attgacctcg
     7681 cgtagcaaac gcttggtggc cctcgctcca cgcccccgga gcgggggcct tttactttag
     7741 gcgctagaat gattggcaag cggcagcgga cgcaggtcgc ccgataattc atgtttctcc
     7801 aggcagccgt tgtccgccgc gatgcgggtt cgggctgcgt ctgcggtctg acgcatagca
     7861 ttgacataca ggcttttttc gagtgacttc gggcggggct tggttcggtc tgatggacca
     7921 gtcgtggggg gacacgtcat gccgtatttc gttttcgggc aggagcggga ttgggacggc
     7981 tacgggcagg atacgttcgt tgtccagatc gaagacccgg accgggtcgc ccacgcgcgc
     8041 ggactgatcg ccggtaccga gacggacagg cctctgattg gcggtgtcgt cacaatggcc
     8101 ccggctgatt acaatatcgg ctggtccttc cacatcgcac cgccggacct cgtcttcgcc
     8161 gacggcttca ccgagatagg cgataacgac atcagggggg tcgaagcggg tgtgtccttc
     8221 tttggcgacg ggtttcttcc gggcaacagg tggacgccct ggaccgtgta catcgtaacc
     8281 gagctgaacc tggtgtccgg tggaggcaac gccgacaatc ttcagggaac gcagctgccc
     8341 gacatcgtct tcggacgggc cgggagggat accctgtccg gaggcgaagg tgacgatcat
     8401 cttatcggcg agggaggaat ggaccgtctc cagggcggca tcggacgtga caaactgtcg
     8461 ggcggagacg gcgccgatag tctgaacggc ggcgcgggca aggacatcct cgatggcggc
     8521 ctcggcgccg acaccatcgc gggcggtacg ggcaacgaca cctaccacgt gcgcgcaggc
     8581 gaggatgtca tcaccgaagc ggctgccggc ggtgtcgacg cgatatggag ctggatcagc
     8641 tacactcttg cccatcagaa tgtcgaagat ctgaggctga tggatagcgg aacggcgaac
     8701 atcgacggga ccggcaacgc gctcgacaac cgcatctggg gcaattggga gatcaatgtg
     8761 cttcgcggtc gcgagggcaa cgatactctg gacggtcacg ccggcaatga cgtcctggtg
     8821 ggcggcaacg gcaacgacac gctcatcggc ggcggcggcg attctgacac ggccagctat
     8881 gcctctgcgg ctggggcagt gagggcgaac ctggcgctga cgacccccca ggacacgctg
     8941 ggcgccggca ccgacacatt tgcggcaatg ccttgggaga cgatgacaag catcgagaac
     9001 gtggtcggat cggcttttgc agacaggttg cgcggcaaca gcgaggcgaa tagcctcaat
     9061 ggcggccccg gtgcggacag gctggaaggc ggtgcgggca atgaccgcct caacggcggc
     9121 ggcggcaacg acagccttca cggcggggcc ggcaacgata ggctcaccgg cggcacgggc
     9181 gaggacgcct tctggttcga tgcggcgccc gaggcctcca acgtcgaccg gatccttgac
     9241 ttctcggccg cgcgcgacac cctcttcctc gaccgttcgg ccttcgatgc gatcggcgcc
     9301 gacggcacgc tcgccgcctc cgccttccat gccgggaccg ccgcccacga tgcctccgac
     9361 cggatcgtct acgacgccgc cacgggcaag atcttctacg acgccgacgg ctcgggcgcg
     9421 ggcgccgcga tcctgttcgc cacggtcgat acagagacga gcctcaccca cgccgacttc
     9481 aacatcgtgg cctgaccgta gcggcggcga gcaagtgaat gtctgctttg ccggacgaac
     9541 cgcgcgtgtc gacagccccg gcggctccgg ttagatcgaa atgatggctt ccaacctcaa
     9601 aatcgcctcc tggaacatca actcggtccg cgcgcggatc gagatcgtcg agaagttcct
     9661 gcgcgaggag cggccggaca tattgtgcct gcaggagacc aaggtcgtcg acggcgactt
     9721 cccgcgcgcc gccttccacc ggctcggcta ccgccacatc atcctgtgcg gccagcgcat
     9781 gcaccatggc gtcgcgatca tctccaggat cccgatgcgc gaggacgacc ggctcgactg
     9841 gcaggcgaac ggcgaggccc gccattgcgg cgtgcagctg gagtgcggca tccgcctcga
     9901 gaatgtctac gtgcccgccg gcggcgacgt cccggaccgg acggtcaacc cgaagttcgg
     9961 gcagaagctg gacttcatcg aacggatgac gcgctggtcc gagcagtgcc ggcggccgac
    10021 cttgctggta ggagacttca acgtggcgcc gctcgaatgc gacgtctgga gccacaagca
    10081 gctgttgggc gtcgtcagcc acacgcccgt cgaggtcgaa gcgctcaccc gg
//
LOCUS       DASVRK010000118        12023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_518602, whole genome shotgun sequence.
ACCESSION   DASVRK010000118 DASVRK010000000
VERSION     DASVRK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518602"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VF589_04410"
     CDS             <1..810
                     /locus_tag="VF589_04410"
                     /inference="COORDINATES: protein motif:HMM:NF017013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEX8256851.1"
                     /translation="RALTDALLRSGAGISEINIVRRHLSRLKGGRLGAAAQPARLVTL
                     AISDVPGDRPEDIGSGPTVADPSTAEAAAAVLAAYGLSAPAPLIESVEPGEAAGWRGD
                     YRIVAGGGDALQAASAAAAALGYRPVIVDPCATGEAGAVARAHAALALAAAPGSALIS
                     GGELGVAVRGAGRGGRNAHYALALALALRGAPGIHALAADTDGIDGNSAAAGAFIDPG
                     TLARAAAHGRDPALALEACDAGGLFEALGDALTTGPTGTNVNDLRIILVDP"
     gene            820..1689
                     /locus_tag="VF589_04415"
     CDS             820..1689
                     /locus_tag="VF589_04415"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX8256852.1"
                     /translation="MSEWTIGIIGGSGLYAIDAVQDAQWIEVDTPWGRPSDALLTGRI
                     HGVKFVFLPRHGRGHRLPPSAVNARANIDALKRAGCTDLIAVSAIGSLRAELPPGRFV
                     IVDQFIDRTFARAKSFFGPGMVAHVSMAEPVCPRLSAMAAAAAEAAGADVARGGTYLA
                     MEGPQFSSRAESELYRSWGCDVIGMTAMPEAKLAREAELPYALVGMVTDYDCWRGGGE
                     QVEVAAIIAQLTANAATAGAMVAELARALPAVREPSPIDTALDTALITASDARDPALL
                     AKLDAICGRVLGG"
     gene            complement(2077..2784)
                     /locus_tag="VF589_04420"
     CDS             complement(2077..2784)
                     /locus_tag="VF589_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX8256853.1"
                     /translation="MRPLLRARRSQLLGLMLSVVIPALNAGACLRACLAALGEVDELI
                     LVDGGSRDDTIAIAEAARARVIVSPKGRGVQLRAGGDAARGDWLLFLHADTLLEPGWA
                     EAARAHMLRHPRKAACFRLRLDDPAWQARAIERGVAARVRLLGLPYGDQGLLVPRALY
                     ERAGGFRPLPLMEDVDLVRRIGRRRLRLLAAGAVTSAERWRRDGWLRRSARNLACLGL
                     YTVGVPAERIARLYERR"
     gene            complement(2739..3695)
                     /locus_tag="VF589_04425"
     CDS             complement(2739..3695)
                     /locus_tag="VF589_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8256854.1"
                     /translation="MATAPNLSPFTDPAARPLDPAKFRDPRVTAKGEPRAHVDLDRLD
                     TLWLNTGTLCNLACKSCYIESSPTNDALVYLTLAEAEAFFEEAAALGTREIGFTGGEP
                     FMNRDLVPMLRSALGRGFEVLVLTNAMRPMRRFEDALKALVLEHRERLTMRVSLDHHS
                     RAVHEAERGAGTWDKALDGFKWLSDHGFRIAVAGRRFGGESEAEGRESYDRLFRRLGV
                     NVGAGLVLFPEMDAAADVPEISEACWGILGVAPGAMMCASSRMVVKHKGADRPIVVAC
                     TLLPYDPQFALGDTLAGARRKVMLNHPHCARFCVLGGASCSG"
     gene            complement(3761..4348)
                     /gene="msrA"
                     /locus_tag="VF589_04430"
     CDS             complement(3761..4348)
                     /gene="msrA"
                     /locus_tag="VF589_04430"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8256855.1"
                     /translation="MRNLLILLAALFAAFTAAAAQSPAPRASAVFAGGCFWCTESDFD
                     KIPGVVSTTSGYIGGKVANPTYEQVSAGGTGHIEAVRIVYDPTRVSYATLASRFFRTI
                     DPLDSGGQFCDRGYQYRSAIFVADAAQRSAALEAKAKAAAALKKPVATLILPAARFYP
                     AEGYHQDYYKKNPVRYRFYRYNCGRDARLKRVWGG"
     gene            complement(4372..4869)
                     /gene="msrB"
                     /locus_tag="VF589_04435"
     CDS             complement(4372..4869)
                     /gene="msrB"
                     /locus_tag="VF589_04435"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8256856.1"
                     /translation="MTQFGLTKRNFLGSAAAAMCALVVGCARTGDAAEAETFEITKSP
                     EQWREILTPQQYAVLRQASTERAGSSPLDREHRRGTFLCAGCDLPVYSSEAKFDSGTG
                     WPSFTAPIAGAVRTKEERGLFGPQTEVHCRRCGGHLGHVFDDGPPPTGQRYCMNGIAM
                     KFVAA"
     gene            complement(4922..5140)
                     /locus_tag="VF589_04440"
     CDS             complement(4922..5140)
                     /locus_tag="VF589_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256857.1"
                     /translation="MDGQQHVLDDVVDGILGPAAPPGERANERDDRLQEQAVGAGVAA
                     LAGGEKLAPAFVARDAAIADPPFLSAPV"
     gene            5051..5518
                     /locus_tag="VF589_04445"
     CDS             5051..5518
                     /locus_tag="VF589_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8256858.1"
                     /translation="MPFVRTLARRRSWSEDAVDDVVQDVLLTVHRVRHTFEPGQPVKP
                     WLTAIVARRSIDALRRRSRIGAREVNDQPAYETFADPAANKEEAGDPARLLAGMTAAL
                     SPGQKEALELVKLREMSLADASAASGQSVASLKVNIHRAIKKLRSNFGKGPAE"
     gene            5515..6171
                     /locus_tag="VF589_04450"
     CDS             5515..6171
                     /locus_tag="VF589_04450"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEX8256859.1"
                     /translation="MSGGGVDELIDKLAADARPVKPLAPPLRRALATLAAAALACGIA
                     IFWLGDVGQLLTRYAGRELLMSLEMAAMLATGLLAVTGAFFLSVPGRSRYWLLAPLPP
                     FAAWLMLSGLGCYRELIRSGTGGGAIGHSLDCLLFIAASSLLLGAPLAWRLSRARPIE
                     PLAVTTLGGLGAAALAAFLLQFFHPFAVTFVDLAVHVAAILLVVGTSALINRRALIPA
                     "
     gene            6225..6938
                     /locus_tag="VF589_04455"
     CDS             6225..6938
                     /locus_tag="VF589_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX8256860.1"
                     /translation="MPHKSFTRLALVGLTTIAGLAPASSALAADADHPTIVELYQSQG
                     CSSCPPANAVLNSIAGRPDVLALSFAVTYWDRLGWKDVFGHPVFTARQWEYANAAGRG
                     KVFTPQVIVNGRGVLVGSRKAEVEAFIKRYDRGASGPAIAASGDEVSIGKGRAAGPAN
                     IWLVRYDPRTHNVPIRAGENGGRTLPHRNIVRTLHLLGKWNGEAASFKMPTLPDRAYK
                     SAVLVQDGPGGAILSGGRI"
     gene            6942..7391
                     /locus_tag="VF589_04460"
     CDS             6942..7391
                     /locus_tag="VF589_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256861.1"
                     /translation="MEATRFKALSLLLLRIGTGLLLVLWGSLRLMSPEAGPGLANKYY
                     GGLLDLQAIQFAFGAAEVAIGALVVLGLFRRIAYLLQALILVPGTLMLWRYLLDPLGQ
                     YLLDEESSQILFFPSITVAAATLVLLAFRDEDRLSLDAKRARAGAAG"
     gene            7388..9532
                     /locus_tag="VF589_04465"
     CDS             7388..9532
                     /locus_tag="VF589_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8256862.1"
                     /translation="MKRIGGGRLALLLLLTGAIAAFFILDLGQYLTLDSLKARQSELA
                     RLVEERLLLAVAAFSLLFVAVTALSLPGAAIMTLAAGAIFGLWLGTLIVSFASAAGAS
                     LAFLSSRYLLRDWVKAKFGRRVEAIDRGIAKDGAFYLLTLRLIPAFPFFLINLAMGLT
                     AMRVGAFYIVSQLGMLPGTLVYVNAGTQLARIESVGDILSPALIGSFVLLGIFPLIAK
                     FVVGGIRRRRVYKGYRRPKKFDRNLIVIGAGAGGLVTAYIAATVRAKVTLIEKGKMGG
                     DCLNTGCVPSKALIRTARLAHEIRTAAEYGLDPAEPRFRFAAVMERIAETIRIIEPAD
                     SVERYTALGVDVRLGHARIVDPWTVEIDGGERLTSRSIVIAAGGEPFVPDIPGLAEAG
                     YLTSDTMWDALSTREEMPGRIAIVGGGPIGTEMAQAFARLGAAVTMVEGGERLLAKED
                     EEVSALVEARLGAEGVDIRTKHMALRCDGKTLIAKDPAGGEVAIPFDEIIVAVGRKAR
                     LSGYGLEELGIETSRTIVTNDYLETRFPNIFAAGDVAGPYQFTHFAAHQAWYAAVNAL
                     FGSLRKFRTDYSVLPWVTYTDPEVAHVGHNELSARAAGVAYEVARYDVGHLDRAVAEG
                     ARSGFVKLLVQPGNDRLIGATIVAANAGELIAEYALAMKHGIGLNKILGTIHAYPTMA
                     EANKYAAGEWKKAHKPERLLGWIERYHGWRRG"
     gene            9532..10122
                     /locus_tag="VF589_04470"
     CDS             9532..10122
                     /locus_tag="VF589_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX8256863.1"
                     /translation="MRILIFAKAPVAGAVKTRLIPALGAEGAAKLARTMLIETCREAV
                     AAEVGPAELCLAGDRACIPELPEGVALSDQGEGDLGARLWRAAERAGPPLLLIGTDCP
                     GLDRGRLRSAAEALRQHDAVLHPTEDGGYALLGLNRLDPSLFTDVAWSTASVAGTTIG
                     RIEGLGWSVFLSETLRDIDEPVDLVAYGTSPDTSSG"
     gene            10154..10537
                     /locus_tag="VF589_04475"
     CDS             10154..10537
                     /locus_tag="VF589_04475"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8256864.1"
                     /translation="MTSLPPYAELLGLSTHRDGDGGLIWVMPFRNAVVGRPGFLHGGA
                     IAGLLEFAAFGALYDELGAGAVTLKPINVTVDFKRGGTEHETYAAATVTRLGNRVANV
                     EAYAWQQDRAKPIASARMNVLLRRS"
     gene            complement(10548..11663)
                     /locus_tag="VF589_04480"
     CDS             complement(10548..11663)
                     /locus_tag="VF589_04480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8256865.1"
                     /translation="MPLYLTDDQEMLRDTARSFMAEEGAIGTQLRHWRDTGCKDGFGH
                     ALWKQFAELGFTGILVPEADGGLGLGHVEAGIVLEEIGRNLTPSPFLVTAVAVVEALK
                     GTVHGARWFPGIVSGDTVAALAVDEGAKHRPEAIAMTAERAGNGFRLSGAKQFVVQGG
                     SADLILVAARAEEGVTLFAVEKEVSGLAVEGVRLADSSMAARMTFEGVEVDADAVVGE
                     VGGGAAVLARALGAARAGAASEMVGVASGAMAMTVDYLKQRRQFGRLIGEFQVLQQRA
                     AHLYSELEIARAAALKAQQLLDEGSAEAEAMVSVAKAKAGRVSTLAVQEGVQMHGGIG
                     MTDEYDIGLYMKRDRALNELFGDANYHTDKLARMMGY"
     gene            complement(11766..>12023)
                     /locus_tag="VF589_04485"
     CDS             complement(11766..>12023)
                     /locus_tag="VF589_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8256866.1"
                     /translation="ELKAGQAHPAQPSMMKYAGTELNKARHELVMVAGGSNALEWESD
                     GSKGGKAAREWLRTKANSIEGGTSEIQLGIVAKHILQLPGA"
BASE COUNT         1761 a         4120 c         4266 g         1876 t
ORIGIN      
        1 cgggcgctga ccgatgcgct gctccgatcg ggcgcgggga taagcgagat caatatcgtg
       61 cggcgccatc tgtcgcggct gaagggcggc cggctcgggg cggcggcgca gccggcccgg
      121 ctggtgacgc tcgccatttc cgacgtgccg ggcgaccggc cggaggatat cggatccggg
      181 ccgaccgtcg ccgacccaag caccgcggag gcggctgcgg ccgtgctcgc cgcgtacgga
      241 ctttcggccc ctgccccgtt gattgagagc gtggagccgg gcgaggccgc cgggtggcgc
      301 ggcgactatc ggatcgtagc gggcggcggc gatgcgctcc aagcggcctc ggcggcggcg
      361 gcggcgctgg ggtaccggcc ggtcatcgtc gacccgtgcg cgaccgggga ggcgggcgcg
      421 gtcgcgaggg cccatgcggc gctggcgctc gccgccgcgc cgggcagcgc gctgatctcg
      481 gggggcgagc tcggagtcgc ggtgcgcgga gcgggccgcg gcggccgcaa cgcccattat
      541 gcgctggcgc tggcgctggc cttgcgcggc gcccccggca tccacgcgct ggccgccgac
      601 acggacggca tcgacggcaa cagcgcggcc gccggcgcct tcatcgatcc ggggactctc
      661 gcgcgcgccg ccgcccacgg gcgcgatccg gcgctcgcgc tcgaggcgtg cgatgcgggc
      721 gggctgttcg aggccttggg cgatgcgctg accaccggcc ccaccgggac caacgtcaac
      781 gacttgcgca tcatcctcgt cgatccctaa gaaccggcca tgagcgaatg gacgatcggg
      841 atcatcggcg ggtccggcct ttacgcgatc gacgcggtcc aggacgcgca gtggatcgag
      901 gtcgacacgc cctggggccg cccgtccgac gccctgctga ccggccgcat ccacggcgtg
      961 aagttcgtct tcctgccgcg gcacgggcgc gggcaccggc tgccgccgag cgccgtcaac
     1021 gcccgcgcca atatcgacgc gctgaagcgg gccgggtgca ccgacctcat cgcggtgtcg
     1081 gcgatcggct cgctgcgggc ggagctgccg cccgggcggt tcgtgatcgt cgaccaattc
     1141 atcgatcgca ccttcgcgcg cgccaagagc ttcttcggcc ccggcatggt cgcccatgtg
     1201 tcgatggcgg agccggtctg cccgcgcctc agcgcgatgg cggcggcggc ggcggaggcg
     1261 gcgggggcgg acgtcgcgag gggcggcacg tatctcgcga tggaaggacc gcaattctcg
     1321 tcgcgggcgg agagcgagct ttaccgctca tggggctgcg acgtgatcgg gatgacggcg
     1381 atgcccgagg ccaagctcgc gcgcgaggcg gagctgcctt acgcgctggt cggcatggtc
     1441 accgactacg attgctggcg cggcggcggc gagcaggtcg aggtagcggc gatcatcgcc
     1501 cagctaaccg ccaacgcggc gacggccggg gcgatggtgg cggagctggc gcgggcgctc
     1561 cccgcagttc gcgagccttc gccgatcgac accgcgctgg acaccgccct catcaccgcg
     1621 tccgacgcgc gcgacccggc gctgctggcc aagctcgacg cgatctgcgg ccgggtgctg
     1681 gggggctaga gcgttgaaag ttcaaatgaa accaccatca tccgttcgcc ctgggcctgt
     1741 cgaagcctgt cctgagcgac gccgcaggcg gcgtcgaagg ggggggcctg cgggctgcgg
     1801 cgccgagctt ccacggccgc tgcgataacc gggctgccgc tagagcgtca gcccgggtct
     1861 gggcccctgc cttcgcaggg gcacgtgggg tttgccgttc ctgtgcccct gcggaggcag
     1921 ggcccagccc ggcccctgcg aaggcagggg ccgccaacca agcgctctta ggcgtcgcgc
     1981 gggtcgggcc tttcttcttg ttctgaggcg aaaaaaatga agggcttcca caggctcagc
     2041 ccgaacggca acagcgacaa cttacccttc tacgctctag cggcgctcgt agagccgggc
     2101 gatgcgttcg gccggcaccc cgaccgtata gaggccgagg caggcgaggt tgcgggccga
     2161 gcggcggagc cagccgtctc tgcgccagcg ttcggccgag gtgacggcgc cggcggcgag
     2221 caggcgaagg cggcgacggc cgatgcggcg gacgaggtcg acatcctcca tcaaaggaag
     2281 cgggcggaaa ccgccggcgc gttcgtaaag cgcgcgcggg acgagcaggc cctggtcgcc
     2341 ataagggagg ccgagcagcc gcacccgcgc cgcgacgccg cgttcgattg cccgcgcctg
     2401 ccaggccgga tcgtcgagcc tgagccggaa gcaggcggcc ttgcgcgggt ggcggagcat
     2461 gtgcgcgcgc gccgcctcgg cccaccccgg ctccagcaaa gtgtcggcgt ggaggaagag
     2521 cagccagtcg ccgcgcgcgg catcgccgcc ggcgcggagc tggacgcccc tacccttcgg
     2581 cgagacgatc acccgcgccc gcgccgcctc ggcgatggcg atcgtatcgt cgcgcgagcc
     2641 gccgtcgacg aggatcagct cgtccacctc gcccagcgcg gcgaggcagg cgcggaggca
     2701 ggcgccggca ttcagcgcgg ggatcaccac cgacagcatc agcccgagca gctggctccg
     2761 ccgagcacgc agaagcgggc gcaatgggga tggttcagca tgaccttgcg gcgggcgccg
     2821 gcgagcgtat cgccgagcgc gaattgggga tcgtagggga gcaaggtgca ggcgaccaca
     2881 atggggcgat cggcgccctt gtgcttcacc accatccggc tcgacgcgca catcatcgcc
     2941 cccggcgcga cgcccaatat gccccagcag gcctcgctga tctcgggcac gtcggcggcc
     3001 gcgtccattt cggggaacag caccaggccg gcgccgacgt tgacgccgag ccggcggaac
     3061 aggcggtcat agctttcgcg cccctccgcc tcgctctcgc cgccgaacct gcggccggcg
     3121 acggcgatcc ggaagccatg gtccgacaac catttgaagc catccagcgc cttgtcccac
     3181 gtgccggccc cgcgctccgc ctcatggacg gcgcggctgt gatggtcgag gctgacgcgc
     3241 atagtgaggc gctcgcgatg ctccagcacc agcgccttca gcgcgtcctc gaagcggcgc
     3301 atcggccgca tcgcgttggt cagcaccagc acctcgaagc cgcggccgag cgccgagcgc
     3361 agcatcggca ccaggtcgcg gttcatgaag ggctcgccgc cggtgaagcc gatctcgcgc
     3421 gtgccgagcg ccgccgcttc ctcgaagaag gcctcggcct cggccagcgt cagatagacg
     3481 agcgcgtcgt tggtcggcga gctttcgata tagcagctct tgcaggcgag attgcagagc
     3541 gtgccggtgt tgagccagag cgtgtcgagc cgatcgaggt cgacatgagc gcgcggctcg
     3601 cccttggccg tgacgcgcgg atcgcggaac ttggccgggt cgagcggccg cgccgccgga
     3661 tcggtgaagg gagacaggtt cggggcggtc gcgatggttt ctctccgtca gcagcggctc
     3721 atagttgaaa gccggcccgg cctcaaacgc cgccgcgccg ctagccgccc cagacccgct
     3781 tcagccgcgc gtcgcgcccg caattgtagc ggtagaagcg gtagcggacc gggttcttct
     3841 tgtaataatc ctggtggtag ccttcggccg ggtagaagcg cgcggcgggc aggatcagcg
     3901 tcgcgacggg cttcttgaga gccgccgcgg ccttcgcctt ggcttcgagc gcggcgctac
     3961 gctgcgccgc gtccgccacg aagatcgccg agcgatactg atagccgcgg tcgcagaact
     4021 ggccgccgga atcgagcgga tcgatggtgc ggaagaagcg gctggcgagc gtcgcatagc
     4081 tcaccctggt cggatcgtag acgatccgca ccgcctcgat atggccggtg ccgccggcgg
     4141 acacctgctc gtaggtgggg ttggcgacct tgccgccgat atagccggag gtggtcgaca
     4201 ccacccccgg aatcttgtcg aaatcggatt cggtgcacca gaagcagccg ccggcgaaca
     4261 ccgccgaagc gcgcggagcg ggcgattgcg ccgcagccgc ggtgaacgcc gcgaacaagg
     4321 cggcaagaag gataagcagg ttacgcatcg attttcctag ccggaggcgt gtcaggcggc
     4381 gacgaacttc atcgcgatgc cgttcatgca ataacgttgg ccggtcggcg gcgggccatc
     4441 gtcgaacacg tggccgagat gcccgccgca gcggcggcaa tggacttcgg tctgcgggcc
     4501 gaacagcccg cgctcctcct tggtccgcac cgcccccgcg atcggcgcgg tgaagctcgg
     4561 ccagccggtg ccgctgtcga atttcgcttc ggagctgtag accggcagat cgcagccggc
     4621 gcacaggaag gtgccgcggc gatgctcgcg atcgagcggg ctcgatccgg cgcgctcggt
     4681 gctcgcctgc cgaagcaccg catattgctg gggcgtcagt atctcgcgcc actgctcggg
     4741 ggacttggtg atctcgaacg tctcggcctc ggccgcgtcg cccgtgcgcg cgcagcccac
     4801 caccagcgcg cacatggcgg cggcggcgct tccgaggaag tttcttttgg tcaatccgaa
     4861 ttgagtcatg ctggcccttc ccagagtgtc gatccctgtt tcggcggagc ccgctttcag
     4921 gttacaccgg cgctgaaagg aatggcggat cggcaattgc ggcatcgcgg gcgacgaacg
     4981 ctggggcgag cttctcgccg ccggccaaag cggcgacacc ggcgcctacc gcctgttcct
     5041 gaagtcgatc atcccgttcg ttcgcacgct cgcccggcgg cgcagctggt ccgaggatgc
     5101 cgtcgacgac gtcgtccagg acgtgctgct gaccgtccac cgcgtgcgcc acacctttga
     5161 gccaggccag ccggtcaagc cgtggctgac ggcgatcgtc gcccggcgct cgatcgacgc
     5221 gttgcgccgg cgcagccgca tcggcgcccg cgaggtcaac gatcagccgg cctatgaaac
     5281 ctttgccgat cccgcagcga ataaggaaga agcgggcgac cctgcccgct tgctcgccgg
     5341 tatgacggcg gcgctgtcgc cgggccagaa ggaggcgctc gaattggtga agctgcggga
     5401 aatgtcgctc gccgacgcgt ccgccgcttc cggccagtcg gtcgcctcgc tcaaggtcaa
     5461 catccaccgc gcgatcaaga agctgcggag caatttcggg aagggcccgg ccgaatgagc
     5521 ggcggcggcg tcgacgagtt gatcgacaag ctggccgccg acgcgcgtcc cgtgaagccg
     5581 ttggcgccgc cgctgcggcg ggcgctggcg acgctggcgg cggccgccct cgcctgcggc
     5641 atcgcaatct tttggctggg cgatgtcggc cagttgctga cccgctatgc cggccgcgag
     5701 ctgctgatga gtttggaaat ggcggcgatg ctggcgaccg ggctgctcgc cgtcaccggc
     5761 gcctttttcc tgtcggtgcc gggccgctcg cgctactggc tgctggcgcc gctgccgccg
     5821 ttcgcggcct ggctgatgct gagcgggctc ggctgctacc gggagctgat ccgcagcggc
     5881 accggcggcg gggcgatcgg gcacagcctg gattgcctgc tgttcatcgc ggcgtcgagc
     5941 ctgctgctgg gcgcgccttt ggcctggcgg ctgtcgcgcg cgcggccgat cgagccgctc
     6001 gccgtgacga cgctgggcgg gctcggcgcg gcggcgctcg ccgccttcct gctgcaattc
     6061 ttccacccgt tcgcggtcac cttcgtcgac cttgccgtgc acgtcgccgc gatcctgctg
     6121 gtggtgggaa cctcggccct catcaaccgg cgcgccctca ttcccgcctg aattcacccg
     6181 gtccgcggca agccgccgca gcggaccatc gttcggagac aaagatgccg cataaaagct
     6241 tcacgcgcct ggccctggtc gggctcacta ccatcgcggg cctcgcgccg gccagctcgg
     6301 cgctcgccgc cgacgccgac cacccgacca tcgtcgagct ttaccagagc cagggctgct
     6361 cctcatgccc gccggccaat gcggtgctca attcgatcgc cggcaggccc gacgtgctcg
     6421 cgctgagctt cgccgtcact tactgggacc ggctcggctg gaaagacgtc ttcggccatc
     6481 cggtcttcac cgcgcggcaa tgggaatatg ccaatgccgc cggccgtggc aaagtgttca
     6541 cgccgcaagt gatcgtcaac gggcgcggcg tgctggtcgg cagccgcaag gcagaggtcg
     6601 aggcgttcat caagcgctac gaccgcggcg cctccggtcc ggcgatcgcc gcttccggcg
     6661 atgaggtcag catcggcaag gggcgcgcgg cggggccggc caatatctgg ctggtccgct
     6721 acgatccgcg cacccataat gtgccgatcc gcgccggcga gaatggcggg cgaaccctgc
     6781 cgcaccggaa catcgtccgc acgctgcacc tgctgggcaa atggaacggg gaagccgcga
     6841 gcttcaagat gccgacgctc cccgaccgcg cctacaagag cgcggtgctg gtgcaggacg
     6901 gcccgggcgg cgccatcctc agcggcggga ggatctgagc catggaagcc actcgcttca
     6961 aagccctgtc gctgctgctg ctccggatcg gcaccgggct gctgctcgtc ctgtggggga
     7021 gcttgcgcct catgtccccg gaggcggggc cgggcctcgc caacaaatat tatggcggac
     7081 tgctcgacct gcaggcaatc cagttcgcgt tcggcgccgc cgaagtcgcg atcggcgcac
     7141 tggtcgtgct ggggctgttc aggcgcatcg cctatctgct gcaggcgctg atcctcgtcc
     7201 cgggcacgtt gatgctgtgg cgctacctgc tcgatccgct cgggcagtat ttgctcgacg
     7261 aggagagcag ccagatcctg ttcttcccct cgatcaccgt cgctgccgcg accctggtgc
     7321 tgctggcgtt ccgggacgag gaccggctgt cgctcgatgc caagcgggcg cgcgccggcg
     7381 cggccggatg aagcggatcg gcggcggcag gctcgcgctc ctcctcctgc tgacgggcgc
     7441 aatcgccgcc ttcttcatcc tcgacctcgg ccaatacctg accctcgaca gcctcaaggc
     7501 gcggcagtcg gagctggcgc ggctggtcga agagcgcctg ctgctcgccg tcgccgcctt
     7561 ctccctcctc tttgtggcgg tgaccgcgct ttcgctgccc ggagcggcga tcatgacgct
     7621 ggcggcgggg gcgatcttcg gattgtggct gggcaccttg atcgtgtcct tcgcctcggc
     7681 cgccggggcg agcctcgcct tcctctcctc gcgctatctg ctgcgcgact gggtgaaggc
     7741 caagttcggc cggcgggtgg aagcgatcga ccgcggcatc gccaaggacg gcgctttcta
     7801 tttgctcacg ctgcgcctga tcccggcctt cccgttcttc ctgatcaacc tcgccatggg
     7861 gctgacggca atgcgggtcg gcgcctttta catcgtcagc cagcttggca tgctgccggg
     7921 gacattagtc tatgtgaacg ccggcacgca attggcgcgg atcgagagcg tcggcgatat
     7981 cctgtcgccc gcgctgatcg ggtctttcgt gctgctcggc atcttcccgc tcatcgccaa
     8041 gttcgtcgtc ggcgggatcc ggcgccgccg cgtctacaaa ggctatcggc ggcccaagaa
     8101 attcgaccgc aatctgatcg tgatcggggc cggcgcgggc gggctcgtca ccgcctatat
     8161 cgccgccacc gtccgcgcca aggtgaccct gatcgagaaa ggcaagatgg gcggcgactg
     8221 cctcaacacc ggctgcgtgc cgtcgaaggc gctgatccgc accgcgcggc tggcgcacga
     8281 aatccgcacc gcggccgaat atggcctcga ccccgccgag ccccgcttcc gcttcgccgc
     8341 ggtgatggag cggatcgccg agaccatccg catcattgag ccggccgaca gcgtcgaacg
     8401 ctataccgcg ctcggcgtcg acgtccggct cggccacgcc cgcatcgtcg atccgtggac
     8461 ggtcgagatc gacgggggcg agcggctgac gtcgcggtcg atcgtgatcg ccgccggcgg
     8521 cgagccgttc gtgcccgaca ttccggggct cgcggaggcg ggctacctca ccagcgacac
     8581 aatgtgggac gcgctttcca cccgcgagga gatgcccggg cggatcgcca tcgtcggcgg
     8641 cggcccgatc ggcaccgaga tggcgcaggc cttcgcccgg ctcggcgcgg cggtgacgat
     8701 ggtcgagggc ggcgaacggc tgctggcgaa ggaggatgag gaggtttcgg cgctcgtgga
     8761 ggcgaggctt ggcgcggaag gagtcgatat ccggacaaag cacatggcgc ttcgctgcga
     8821 cggcaagacg ctgatcgcca aggaccccgc cggcggcgaa gtcgcgatcc cgttcgacga
     8881 gatcatcgtc gcggtcggcc gcaaggcgcg gctgagtggt tatgggctgg aggagctcgg
     8941 catcgagacg agccgcacca tcgtcaccaa cgactatctg gagacgcgct ttcccaacat
     9001 cttcgccgcc ggcgacgtgg cggggccgta tcaattcacc catttcgcgg cgcaccaggc
     9061 ctggtatgcg gcggtcaatg cgctgttcgg atcgctgcgc aagttccgca ccgattattc
     9121 ggtgctgccg tgggtgacct ataccgaccc cgaggtcgcg catgtcgggc ataacgagct
     9181 ttcggcgcgg gcggcgggcg tggcttatga ggtagcgcgc tacgatgtcg ggcatctcga
     9241 ccgggcggtg gcggagggcg cgcggtcggg cttcgtcaag ctgctcgtcc agccgggcaa
     9301 cgaccgcctg atcggcgcca ccatcgtcgc cgccaatgcg ggcgagctga tcgccgaata
     9361 tgcgctggcg atgaagcacg gcatcgggct caacaagata ttgggcacga tccacgctta
     9421 tccgaccatg gccgaggcca acaaatatgc cgccggcgaa tggaagaagg cgcacaagcc
     9481 cgagcggctg ctcggctgga tcgagcgcta ccatggctgg cggcgcggct gatgcgcatc
     9541 ctgatcttcg ccaaggcgcc ggtggcgggc gcggtcaaga cgcggctgat cccggctttg
     9601 ggagcggagg gcgcggcgaa gctcgcccgc acgatgctga ttgagacttg ccgcgaggcg
     9661 gtggcggccg aagttgggcc ggccgagttg tgcctggccg gcgatcgggc gtgcattccc
     9721 gagcttccgg agggggtggc gctcagcgat cagggcgagg gcgacctcgg cgcccggctg
     9781 tggcgggcgg ccgagcgcgc ggggccgccg ctgctgctga tcggcaccga ctgtcctggc
     9841 ctcgaccgcg ggcggctgcg ctcggcggcg gaggcgttgc ggcagcatga cgcggtcctt
     9901 caccccaccg aggatggcgg ctacgcgctg ctcggcctga accgcctcga cccgtcgctg
     9961 ttcacggacg ttgcctggag caccgccagc gtcgccggaa ctacgatcgg gcggatcgaa
    10021 gggctcggct ggtcggtgtt cctgagtgag acgctacggg acatagacga acccgtagac
    10081 ctggtcgctt acgggacgag tccagacaca agttcggggt gacggcggcg cgcggcgcgg
    10141 ctagcggggc cggatgacgt cgcttccgcc ttatgccgag ctgctcggcc tcagcacgca
    10201 tcgcgacggg gatggcgggc tgatttgggt gatgccattc cgcaacgcgg tggtcggccg
    10261 ccccggcttc ctccatggcg gcgccatcgc cggcctgctg gaattcgctg ctttcggcgc
    10321 gctttacgac gagctcggcg ccggcgcggt gacgctgaag ccgatcaacg tgacggtcga
    10381 tttcaagcgc ggcgggaccg agcacgaaac ctatgcggcg gcgacggtga cccggctcgg
    10441 caaccgcgtc gccaatgtcg aggcctatgc ctggcagcag gaccgcgcca agccgatcgc
    10501 atcggcgcga atgaacgtgc tgctgcgacg cagctgacct cagtccctca atagcccatc
    10561 atccgcgcca gtttgtcggt gtggtaattg gcgtcgccga acagctcatt gagggcgcgg
    10621 tcgcgcttca tgtagaggcc gatatcatat tcgtcggtca tgccgatgcc gccatgcatc
    10681 tggacgccct cctgcacggc cagcgtcgag acccggccgg ccttggcctt ggcgaccgac
    10741 accatcgcct cggcctccgc gctgccttcg tcgagcaatt gctgcgcctt caaggccgcg
    10801 gcgcgggcga tctccagctc cgaatagagg tgggcggcgc gctgctgcag gacctggaac
    10861 tcgccgatca ggcggccgaa ctgccgacgc tgcttcagat agtcgaccgt catcgccatc
    10921 gcgcccgacg ccaccccgac catctccgaa gccgcgccgg cccgggccgc gccgagcgcg
    10981 cgggcgagca ccgccgcgcc gccgccgacc tcgccgacga cggcgtcggc atcgacctcg
    11041 acgccctcga acgtcatgcg cgcggccatg ctggaatcgg cgaggcggac gccttcgacg
    11101 gcgagcccgc ttacctcctt ttcgacggcg aacagggtga cgccctcctc ggcgcgggca
    11161 gcgacgagga tgaggtcggc cgagccgccc tggaccacga actgcttggc gcccgaaagc
    11221 cggaagccgt tgccggcgcg ctcggcggtc atggcgatgg cttcggggcg gtgcttcgcg
    11281 ccttcgtcga ccgcgagcgc ggcgaccgtg tcgcccgaga cgatgcccgg aaaccagcgc
    11341 gcgccgtgga cggtgccttt cagcgcctcg accaccgcga ccgcggtgac gaggaagggc
    11401 gagggggtga ggttgcggcc gatctcctcg agcacgatgc ccgcctcgac atggccgagg
    11461 ccgagcccgc cgtccgcctc ggggacgagg ataccggtaa agcccagttc agcgaattgc
    11521 ttccagagcg cgtggccgaa gccgtccttg cagccggtgt cgcgccagtg ccggagctgc
    11581 gtcccgatcg cgccttcctc ggccatgaac gaccgggcgg tgtcgcgcag catctcctgg
    11641 tcgtcggtaa ggtaaagcgg catttatttc tccgtcactc cggcgaaggc cggagtctca
    11701 gggggtgaag ggagcggtgc aggttttcgc tgagatgccg gccttcgccg gcatgacggg
    11761 aaggttcaag cacccggcag ctgcagaata tgcttggcga cgattccgag ctggatctcg
    11821 ctcgtgccgc cctcgatcga attggccttg gttcggagcc actcgcgcgc tgccttgccg
    11881 cccttggagc cgtcgctctc ccattcgagc gcgttggagc cgcccgcgac catgaccagt
    11941 tcgtggcgcg ccttgttgag ctcggtgcct gcatatttca tcatcgacgg ctgcgcggga
    12001 tgcgcctgcc ccgccttcag ctc
//
LOCUS       DASVRK010000119        16932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_457239, whole genome shotgun sequence.
ACCESSION   DASVRK010000119 DASVRK010000000
VERSION     DASVRK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16932
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457239"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF589_04490"
     CDS             complement(<1..272)
                     /locus_tag="VF589_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolo-quinoline quinone"
                     /protein_id="HEX8256867.1"
                     /translation="MKRIIIALVAAGLVGGCGILGGDKKPKTPTVGTRIPVLSAETGI
                     EVDPALAQAAVMLPPATPNAEWTQSGGSASKSMGHLALGNALARAWA"
     gene            complement(269..994)
                     /locus_tag="VF589_04495"
     CDS             complement(269..994)
                     /locus_tag="VF589_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8256868.1"
                     /translation="MAIKPRDNETFYREVDEELRRDQTSALVKRWGWAVALGVVVLLA
                     AIGGFLYWQHRQEVRAGERGERLTAVLQEIDAGQTKQAVPKLDALANEGSPGHRAAAL
                     LTKAAIAAQAGNDAAAAAGFKAVAEDEALAQPYRELALIRQTAIEFDTLPPAAVIQRL
                     APLARAGNPWFGSAGEMVALAHLKQGKPQLAAPIFAAIAKDDKLPQSMRSRAMQMAGA
                     LGVDAVQDSESTGEAPAAKETAE"
     gene            complement(1151..2008)
                     /gene="panB"
                     /locus_tag="VF589_04500"
     CDS             complement(1151..2008)
                     /gene="panB"
                     /locus_tag="VF589_04500"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX8256869.1"
                     /translation="MSTYTLDTSTSRAHPTPAPMRRLTVPKIQEKKGGEPIVMLTAYT
                     ARMAQLLDPHCDLLLVGDSLGQVIYGLPTTIPVTMEMMCAHGAAVVRGSWHAVVAIDM
                     PFGSYEAGPEQAFQCAARILKETGAAAVKLEGGEAMAETVAFLTARGIPVIGHVGLTP
                     QAVNALGGYRARGRNDAERAKIIADAKAVSEAGCFALVLEGVVEDIAAEATRAVACPT
                     IGIGASAACDGQVLVTEDMLGLFERTPRFVKRYDDLAVRIGAAAEAYASEVRSRSFPT
                     EDQIYRALG"
     gene            complement(2182..2319)
                     /locus_tag="VF589_04505"
     CDS             complement(2182..2319)
                     /locus_tag="VF589_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256870.1"
                     /translation="MDIQDKRRRLRLLIVAATAFGCSIAALSGTLHAQAYQAEVAAEV
                     R"
     gene            2493..4196
                     /locus_tag="VF589_04510"
     CDS             2493..4196
                     /locus_tag="VF589_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8256871.1"
                     /translation="MNDQAQAVAADDAIPSDCVIAVDERGCVTAFNAAAEKAFGYTSA
                     DVIGRPFADLIARRSDEDELREMRKRLQVATEAAKLGTWSFDLRDGRAWYSDRSREIM
                     GLAPDAVIDARLIKGFVHPDDWEKVASPYYHGVPESTVEIEYRILRPSGELRWIYALG
                     AATKGADGVTRTVDGVHVDITGRKTAEMELARSREALHQSEKLAALGSLLAGVSHELN
                     NPLAAIVGQSQMLEEDSRGTPLEARARKISSAAERCARIVQTFLAMARRRPPHRVAID
                     ANKLAAAALELGEYGLRTAGIAVRTEFAAGLPPVEGDSDQLHQVLINLVINAQQAMEM
                     GGGRDNVLTLRTSLTEAGTVAIDICDSGPGIPPDVAARIFDPFFTTKPQGSGTGVGLS
                     FSHGVVAAHGGSLSLMPSERGAAFRVELPVGARPGVAAEEPAPEDRAAPRQRGRALII
                     DDETDVADTLRELVEREGFAVTTVADGASALSAVEEGHDLIFSDLRMPGMGGPEFFRK
                     LRASRPDYAERVAFVTGDTLGAAMADFLADTGRPVLEKPFTIGSVREIIRSMQGRAEP
                     S"
     gene            4207..4920
                     /locus_tag="VF589_04515"
     CDS             4207..4920
                     /locus_tag="VF589_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8256872.1"
                     /translation="MSEGAHIIVVDDDAGVRATVAEYLLRNGFLVSEADGGDGLRKVM
                     AERPIDLAILDINMPGEDGLTLAREIRGMGPAGVIMLTANNEDIDKVVGLEVGADDYI
                     TKPYNPRELLARIRAVLRRVGNGPAHAAATMGREVHVGKCMLNLDSRKLYGPDGAEVP
                     LTAMEFDLLRCFAEHPARVLSRDQLLDMAHHKTEEPFDRSIDTRITRIRRKIEHDPAK
                     PECIKTVRGAGYIFLPACG"
     gene            complement(4942..5115)
                     /locus_tag="VF589_04520"
     CDS             complement(4942..5115)
                     /locus_tag="VF589_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256873.1"
                     /translation="MFVRLTQANNIAIVVNSEQVKYLQDAGGTATRIHFGKDEIAVVD
                     EPMAAVARALAGG"
     gene            complement(5193..6584)
                     /locus_tag="VF589_04525"
     CDS             complement(5193..6584)
                     /locus_tag="VF589_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8256874.1"
                     /translation="MARQPIKRRWIAALLPLLAVAAAPAPAQVAPPVIEAALQSSADH
                     ALARFYAGRDHRTIWIKGNALTPDAARLVDAVRDAELDGMTAGAAVAASLEQAMVRAR
                     SGDPAELARAELMLSRALVDYVQALRRPADIGMNYGDAAVIASPPSLPEILDAAAKAP
                     SLARHVETVSEVNPVYTELRKAFAAWRGRSNAPVVMAARVSGFGDSRPDLERLFRLNL
                     DRARALPMNRSGRYILVDAAAQRLWMYEDGVPRDSMKVIVGKPGHPTPMIASMIHSAV
                     LNPYWNVPPDLVRDRVAPKVIDQGIGYLKTANYEVLSDWSDQARAVDPAGINWRAVAA
                     GQIEMRVRQRPGAGNSMGVVKFPFENRFGVYLHDTPEKELFAKRERNLSSGCVRLEDA
                     PRLARWLLGSEPRSHSAAPEQVVALAKPVPVYITYLTLGVDGDRLAFRNDPYQRDAEP
                     LRQFASNRLAARD"
     gene            complement(6811..7071)
                     /locus_tag="VF589_04530"
     CDS             complement(6811..7071)
                     /locus_tag="VF589_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256875.1"
                     /translation="MLKLIQSKDGRQFAHDPAARGYHVVYRPWEVNHCPGCGRSHWHV
                     GRLSAECAFCATALPLVEAVMQGATGGHSYNRRPHGYQAEAA"
     gene            7274..9127
                     /locus_tag="VF589_04535"
     CDS             7274..9127
                     /locus_tag="VF589_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8256876.1"
                     /translation="MFARREFMLSTAATGALAIAGCAPRDGAIAAAGAAPAAGDAAAD
                     RLLASIAEELLAEYPENATALGLDKGERAPLKARLTDRSLEGRHRLAAAAARRLERLR
                     AVDRAGLSPAAALNVDVVQAAHELAADGFRFPYGDVVVLNQQWSYRNAPYVVAQNTGA
                     FVEIPDFLDSSHKVETAADADAYLARLEGYAAALDGETERLRHDAALGVIAPNFLLDK
                     TLKQMAGARAQPIAQWGLVTSLAGRTRELGGNYGARAERIVADRVAPALDRQIAELKA
                     HRAVATDAAGVWKLPRGEEYYAWALRAATTSTLTPDEVHRTGLEQLRGLQAQMDEILR
                     AQGLSRGSVGERLTALGKDPRHLFPNTDAGRAQILDYINGRVADIRQRLPRAFATLVP
                     GNLIVKRVPPEIEAGAPGGYAAAGTIDGTVPGNYYINLRDTSMWPRYSLPTLTYHEGI
                     PGHIWQGEYTYKLPLVRSLLAFNAYSEGWALYAEQLGDELGAYDGEPLGRLGYLQSIA
                     FRACRLVVDTGLHTKRWTRDKAIQWFAETNGSSIEEVSGEVDRYCAWPGQACGYKVGH
                     SEINRLRDKARAALGQRWDLRSFDDALVLGGAVPLTSLEQVIDAYIAGRRS"
     gene            9224..9964
                     /locus_tag="VF589_04540"
     CDS             9224..9964
                     /locus_tag="VF589_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8256877.1"
                     /translation="MSFDNAEVNALLRAVEPAVRGQFKAQLRPIDLRRHHVLYNLGTE
                     LDRVYFPLSGLVGIRAETAEGEFIESAIVGREGSIGAFEACGSRQYSAEAVVQVPGRA
                     LAMSAAAYRDLFDASPALRTAVHRYVEQLMSETRQFVVCNTLHSVEQRLSRTVLEALE
                     KSGLDDTLPLTQQALARMLGVQRTTVALILSRLQREGITNGRRGVLRVIDLDGLERLA
                     CSCRLSLKATREAIASARQPACEAVVAA"
     gene            10080..10502
                     /locus_tag="VF589_04545"
     CDS             10080..10502
                     /locus_tag="VF589_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX8256878.1"
                     /translation="MISLFYVSRSAVPPDQEAEQLAGIGAVAQRRNLAFGVTGALVLV
                     DRIFAQVLEGPEAEVNQLMVSILRDRRHSDVRIIEMAPIDERRFATWSMAFVPPSAAT
                     GRLLRALADPRTDLRAGSPVAELLDLMAAGAGSFTRFR"
     gene            complement(10486..11373)
                     /locus_tag="VF589_04550"
     CDS             complement(10486..11373)
                     /locus_tag="VF589_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8256879.1"
                     /translation="MIRPHLPLNALRAFEAAARHLSFTRAAIELCLTPAALSHQVKGL
                     EQRLAAPLFRRLPRGLALTEEGQALLPVLRDAFDRIAEMLQRFDAGVVREVLTVGAVG
                     TFAVGWLLPRLPRFHAAHPFVDLRLSTHNNRVDIAAEGLDLAIRFGDGAWHGTEAMRL
                     FAAPLTALCTPEVAARLGDPADLGREVLLRSYRAEDWPDWFAAAGVPAPAVRGPVFDS
                     SWVMIEAALQGAGVALAPAAMFERELAEGALARPFEVQVDTGSYWLCRLKSKERTRAM
                     RAFEEWIRAEAAAPASGSG"
     gene            11471..12214
                     /locus_tag="VF589_04555"
     CDS             11471..12214
                     /locus_tag="VF589_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256880.1"
                     /translation="MIERREFTRGLCASIGAYALIEAVAGARALARPSRAAMSWVDAQ
                     QQIAAALAAGRIAPRDWQREVEQLAGEVHLPELLAATDYARLKTSFDFKGGMPSKRFV
                     RFGEGAPALSYGLAFFGFRRGQAITPHAHRNMVSAHMVVAGALRVRTFDRLRDEPGAL
                     IVRPTLDRRIALSDVSTMSSQRDNVHWFVAEQDDSATLDVIVDALEPGRDAYTIELID
                     PLGGRALGDGSLRAPIINWEESVSKYGRA"
     gene            12201..13094
                     /gene="bla"
                     /locus_tag="VF589_04560"
     CDS             12201..13094
                     /gene="bla"
                     /locus_tag="VF589_04560"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HEX8256881.1"
                     /translation="MAAPDLLAALVAALALAAPAAPAPGSIEGLSASDAKAAAPAPPF
                     RIADNLYYVGSRETAVYLITGGDGLILLDGGFEAMAPQVLANVEALGFDPARIRLLIN
                     SQAHFDHAGGLAAIKARTGAAMAASAEDAALLARGGRGDFAWGDKLPYPAVKADRIVQ
                     HGETVKLGDIRLTARLTPGHSKGCTTWQLRTGDRGKAYDALFLCGTSAPGYRLAGNPL
                     YPRIAEDFRRSFATWRALPCDIFLGAHGSYYGIDAKRAALAAGAARNPFVDPEGCRAH
                     IDGAEQTFERQLREQQAAGPR"
     gene            complement(13095..13469)
                     /locus_tag="VF589_04565"
     CDS             complement(13095..13469)
                     /locus_tag="VF589_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8256882.1"
                     /translation="MLLTDTHALIWFAADDHRLSRAARAALVDPQQEVFVSVVCYWEI
                     EVKRLRSSEFSPPEPVRALMDRAGFRPLGMDFDVPVRLAGLPRVHGDPFDRMLVAQAL
                     HHGLTLVTGDRALRRYPVPTLW"
     gene            complement(13456..13755)
                     /locus_tag="VF589_04570"
     CDS             complement(13456..13755)
                     /locus_tag="VF589_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX8256883.1"
                     /translation="MTIHVNIGAAKTRLSELLAAAKRGEEVIITRAGSPEARLVPVDP
                     DQHLRDLAEKRRKALGSWKGRINDDIDWFAPMSEEELAQWYDAPIFPDLDGDAAD"
     gene            complement(13801..15375)
                     /gene="guaA"
                     /locus_tag="VF589_04575"
     CDS             complement(13801..15375)
                     /gene="guaA"
                     /locus_tag="VF589_04575"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX8256884.1"
                     /translation="MTDQTPASPAADTILIVDFGSQVTQLIARRVREAGVYSEIAPFN
                     AAAEAFERLKPKGIIFSGGPSSVTWEDSPRAPQQLLEAGLPVMGICYGQQTMAQQLGG
                     SVAPSDNREFGRAFIDITGQSSLFDGLWSPGERHQVWMSHGDRVERLPDGFKVVAQSE
                     GAPYAVAVDEERRFYSLMFHPEVVHTPDGGKLIANFVRHVCGLAGDWTMAEFREAKIA
                     EIRAQVGSGRVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLMRLGEAEQVVSLF
                     RNSYNIPLVHVDAEDLFLSGLAGLDDPEAKRKFIGKTFVEVFEEEAGRIGGADFLAQG
                     TLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRVLGRELGL
                     PEAFVGRHPFPGPGLAIRIPGEVTKEKCDILRKADAIYLDEIRMAGLYDAIWQAFAVL
                     LPVRTVGVMGDHRTYDSVCALRAVTSTDGMTADIYPFDATFLSRVATRIINEVRGINR
                     VVYDYTSKPPGTIEWE"
     gene            15493..16536
                     /locus_tag="VF589_04580"
     CDS             15493..16536
                     /locus_tag="VF589_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8256885.1"
                     /translation="MPRRIKALLDQLESEVGRFAQANERIAKQTNFLALNATIEAARS
                     GDAGRGFAVVAQEVKALAAQARAASNDFRALVIDRLKTGSTIADSLVDDIEGSRLAEF
                     AFATVQTFARNIYSRSSDIRILATDSDIVAAARAPDDRALIARAEDRLRAAMRFSPHY
                     VNAVFATRAGKVVGSADPRSPLLGVDVGAEPQFNNVIQSRSTDDWFTDAVWENPYSNG
                     RQVLIFACGIRALEHDGQPAEGCLYLEYDWQGNAAEQLQAQLAAADKEIRSATLRIME
                     HGGRIVASTLPGEFDKHYALPENRPARGMISKGDRVVAYADANDRGGRNSLNLTCVIE
                     HFIESGGEAAIAA"
     gene            complement(16547..>16932)
                     /locus_tag="VF589_04585"
     CDS             complement(16547..>16932)
                     /locus_tag="VF589_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256886.1"
                     /translation="GGNDVLAGGRGADQLTGGGGADRFDFNTLLNPVSNVDTITDFSA
                     PQDTILLDRTVFAGIAVDGQLAASGFGLGTSAQDRTDRIVYDAGSGSIFYDSDGIGGA
                     GAILFAKVNPNTPLTNADFTAYMGG"
BASE COUNT         2645 a         6148 c         5557 g         2582 t
ORIGIN      
        1 gcccaggccc gcgcgagcgc gttcccgagc gccagatgac ccatcgactt ggacgcgctg
       61 ccccccgact gcgtccattc ggcattgggc gtcgccggcg gcagcatcac cgccgcctgg
      121 gcgagcgccg gatcgacctc gatgccggtt tcggccgaca gcacggggat ccgggttccg
      181 acggtcggcg tcttgggctt cttgtcgccg cctaagatgc cgcagccgcc gaccaggccg
      241 gccgcgacga gggcgatgat gatccgcttc attcagctgt ttccttcgct gcaggggctt
      301 cgcccgtgct ttcagaatct tgaactgcat ccacgcctag cgcaccggcc atctgcatgg
      361 cgcgcgagcg catgctttgc gggagcttat cgtctttggc gatagcggca aaaatcggcg
      421 ccgccagttg cggcttgcct tgcttcagat gcgcgagcgc gaccatttcg ccggcgcttc
      481 cgaaccacgg gttgccggcg cgcgcgagcg gcgccagccg ctggatcacc gccgcggggg
      541 gaagcgtgtc gaactcgatc gcggtctggc ggatcagcgc cagctcgcga tagggctgcg
      601 ccagcgcctc atcctcagcc acggccttga agccggccgc ggcggcggca tcgttgccgg
      661 cctgggccgc gatcgccgcc ttggtcagca gcgccgcggc gcggtggccg gggctgcctt
      721 cgttggcgag cgcatcgagc ttcggcaccg cctgcttggt ctggccggcg tcgatctcct
      781 ggagcacggc ggtcagccgc tcgccgcgct cgccggcgcg cacctcctgc cggtgctgcc
      841 agtaaaggaa gccgccgatc gccgcgagca gcaccaccac gccaagcgcg acggcccagc
      901 cccagcgctt gacgagggcg gacgtctgat cgcggcgcag ctcctcgtcc acctcgcggt
      961 agaaggtctc attgtctctg ggcttgatcg ccaaacaaag tctccacggt caccagggtc
     1021 ggccgctcgc ccatcttccg gcaacagctt cgccccgttc cgaaacagca gcgaagatga
     1081 acagaagatt ggcgcccggg cggtgcccct gccctcctct accggctcga accgccaaac
     1141 gaaaggctct tcacccgagc gcgcggtaga tctggtcttc ggtcgggaag ctgcgcgacc
     1201 ggacctcgct cgcataggct tcggcggcgg cgccgatccg gacggcgagg tcgtcatacc
     1261 gcttgacgaa gcggggcgtc cgctcgaaca ggccgagcat gtcctcggtg accagcacct
     1321 gcccgtcgca ggccgccgaa gcaccgatcc cgatcgtcgg gcaagccacc gcgcgggtcg
     1381 cttccgcggc gatatcctcg acgacgccct cgagcaccag cgcgaagcag ccggcctccg
     1441 acaccgcctt ggcgtcggcg atgatcttcg cgcgctcggc atcgttgcgg ccgcgcgcgc
     1501 gatagccgcc gagcgcattc accgcctgcg gggtgagccc gacatggccg atgaccggga
     1561 tgccgcgggc ggtgaggaag gcgaccgtct cggccatcgc ctcgccgccc tcgagcttga
     1621 ccgcggccgc gccggtctcc ttgaggatgc gcgcggcgca ttggaacgcc tgttccggcc
     1681 ccgcctcgta ggagccgaac ggcatgtcga tcgccaccac cgcatgccag gagccgcgca
     1741 ccaccgccgc gccatgggcg cacatcatct ccatcgtcac cgggatggtc gtgggcagcc
     1801 cgtaaatcac ctggccgagg ctgtcgccga ccagcaagag gtcgcaatgc ggatcgagca
     1861 actgcgccat tcgcgccgta taggcggtca gcatcacgat cggctcgccg cccttcttct
     1921 cctggatctt ggggaccgtc agccgccgca tcggcgcggg cgtcggatgg gcgcggctgg
     1981 tcgaggtgtc gagcgtgtag gtggacatgg ccggccttta gcctttccgc tcccgcctga
     2041 ccagattgcc ggcgagtctc ccgatcgcgg cttcggcaaa cgcaacagcc tccggacgcc
     2101 ggggggcggc gtccgaaggc tgcgacaggc tcgacgcctg tttcgtctat ctgtgcttgc
     2161 tcccccccat cgaggagcgg cttatcggac ctcggccgcg acctcggcct gataggcctg
     2221 cgcgtgaagt gtaccactaa gcgcggcaat gctgcaaccg aaggcggtcg cagcgacgat
     2281 gagcaggcgc aggcgccgcc ttttgtcctg gatatccatc ttcgaaagtc tccacagacg
     2341 cttacgtccc gcgtctgtgg cgaaactatc gccgcgccac gtttcgggat gatgccggcc
     2401 acgcggtttt tttgtttcaa ttgttacaga ggccggtttt cgtggtagga gatgggcagc
     2461 aaaaaggggc cgagtcttcg ggggaacgac cggtgaatga ccaggcgcag gcagtggcgg
     2521 cggacgacgc gataccctcc gactgcgtga tcgcggtcga cgagcgcggc tgcgtcaccg
     2581 ccttcaacgc ggccgccgaa aaagccttcg gctataccag cgcggacgtg atcggccgtc
     2641 cgttcgcgga cctcatcgcg cgccgctccg acgaggacga attgcgcgag atgcgcaagc
     2701 gcctgcaggt cgcaaccgag gcggcgaagc tcggcacctg gtcgttcgat cttcgcgacg
     2761 gccgcgcctg gtattcggac cgctcgcgcg agatcatggg cctcgccccc gacgcggtga
     2821 tcgatgcgcg gttgatcaag ggcttcgtcc acccggacga ttgggagaag gtggcctcgc
     2881 cttattatca tggcgtcccc gaaagcaccg tagagatcga gtaccgcatc ctccgcccgt
     2941 ccggcgagct gcgctggatc tatgcgctcg gcgccgccac caagggcgcg gacggcgtca
     3001 cccgcaccgt cgacggcgtc catgtcgaca tcaccggccg caagaccgcc gagatggagc
     3061 tcgcccgctc gcgcgaggcg ctccaccagt cggaaaagct cgccgctttg ggctcgctgc
     3121 tcgccggcgt cagccacgag ctcaacaacc cgctcgccgc gatcgtcggc cagtcgcaga
     3181 tgctcgagga ggacagccgc ggcacgccgc tcgaggcgcg ggcgcgcaag atcagctcgg
     3241 ccgcggagcg ctgcgcgcgc atcgtccaga ccttcctcgc catggcccgc cgccggccgc
     3301 cgcatcgggt cgcgatcgac gccaacaagc tcgccgccgc cgcgctcgag ctcggcgaat
     3361 atggcctgcg caccgccggc atcgccgtcc gcaccgaatt cgccgccggc ctgccgccgg
     3421 tggagggcga cagcgaccag ctccaccagg tgctgatcaa cctcgtcatc aacgcgcagc
     3481 aggcgatgga gatgggcggc gggcgcgaca atgtgctgac cctgcgcacc tcgctcaccg
     3541 aggcggggac ggtcgcgatc gacatttgcg acagcggccc cggcatcccg cccgatgtcg
     3601 cggcgcgcat cttcgacccg ttcttcacga ccaaaccgca aggctcgggg accggcgtcg
     3661 gcctctcctt ttcgcacggc gtggtcgccg cccatggcgg cagcctcagc ctgatgccga
     3721 gcgagcgcgg cgccgccttc cgggtcgagc tgccggtggg ggcgcggccc ggcgttgcgg
     3781 ccgaggagcc cgctcccgag gaccgcgccg ctccccgcca gcgcggccgg gcgctgatca
     3841 tcgacgacga gaccgatgtc gccgacacct tgcgcgaact ggtcgagcgc gagggcttcg
     3901 ccgtcaccac cgtcgccgac ggcgccagcg cgctctccgc cgtcgaggaa ggtcacgacc
     3961 tcatcttctc cgacctgcgc atgcccggca tgggcggccc cgaattcttc cgcaagctgc
     4021 gcgccagccg cccggattat gccgagcggg tggcattcgt gaccggcgat acgctcggcg
     4081 cggcgatggc cgacttcctc gccgacaccg gccgccccgt gctcgaaaag ccgttcacaa
     4141 tcggctcggt ccgggagatc atccggtcga tgcagggacg cgccgaacca tcatgagcaa
     4201 agcggcatga gcgagggcgc ccacatcatc gtcgtcgacg acgatgccgg cgtccgcgcc
     4261 accgtcgccg aatatctgct gcgcaacggc ttcctcgtca gcgaggccga cggcggcgac
     4321 gggctgcgca aggtgatggc cgaacggccg atcgacctcg ccatcctcga catcaacatg
     4381 ccgggcgagg acggcctcac gctcgcccgc gagatacgcg gcatggggcc ggccggcgtg
     4441 atcatgctga ccgccaacaa tgaggatatc gacaaggtgg tcgggctcga ggtcggcgcc
     4501 gacgattata tcaccaagcc ctataatccg cgcgagctgc tcgcccggat ccgcgccgtg
     4561 ctccgccgcg tcggcaacgg ccctgcccac gccgccgcga cgatgggccg cgaggtgcat
     4621 gtcggcaagt gcatgctcaa cctcgacagc cgcaagcttt acggcccgga cggcgccgaa
     4681 gtgccgctga ccgcgatgga gttcgatctc ctgcgctgct tcgccgagca tcccgcccgc
     4741 gtcctcagcc gcgaccagtt gctcgacatg gcccatcaca agaccgagga gccgttcgac
     4801 cgttcgatcg acacccgcat cacccgcatc cgccgcaaga tcgagcacga tcccgccaag
     4861 cccgaatgca tcaagacggt gcgcggcgcc ggctacatct tccttccggc ctgcggctga
     4921 ccggctgcga gcggcctgtg ctcagccgcc ggcgagcgcc cgcgcgaccg ccgccatcgg
     4981 ctcgtcgacg acggcgatct cgtcctttcc gaaatggatc cgcgtcgcag tgccgcccgc
     5041 gtcctgcaga tatttgacct gctcggaatt caccacgatc gcgatgttat tggcctgggt
     5101 cagccgtacg aacatagcct gtctcctcgg ttctggttgg ctcatcctgc cataggcggc
     5161 ttcgaaccgt caccccgaac cggcggagcc cttcaatccc tcgccgcgag ccggttcgag
     5221 gcgaactggc ggagcggctc ggcatcgcgc tggtagggat cgttccggaa ggcgagccgg
     5281 tcgccgtcga cgccgagggt gagataggtg atgtagaccg gtaccggttt ggcgagggcg
     5341 actacctgtt cgggcgcggc cgagtggctg cgcggctccg atccgagcag ccaccgcgcc
     5401 agcctggggg cgtcttcgag ccggacgcag ccggagctga ggttgcgctc acgcttggcg
     5461 aacagctcct tttccggagt gtcgtgcaga tagacgccga agcgattctc gaacgggaac
     5521 ttgaccacgc ccatggagtt gccggcgccc ggccgctgcc ggacccgcat ctcgatctgc
     5581 ccggctgcga cggcgcgcca gttgatcccg gccggatcga ccgcccgcgc ctgatcggac
     5641 caatcggaca gaacctcgta attggccgtc ttcaaatatc cgatgccctg gtcgatcacc
     5701 ttcggcgcga cgcggtcgcg gacgagatcg ggcggcacgt tccaataggg attcagcacg
     5761 gcgctgtgga tcatgctggc gatcatcggc gtgggatggc cgggcttgcc gacgatgacc
     5821 ttcatgctgt cgcgggggac cccgtcctcg tacatccaca gccgctgcgc ggccgcatcg
     5881 acgaggatat agcggccgct gcggttcatc ggcagcgccc gcgcccggtc gaggttgagc
     5941 cggaacaggc gctccagatc gggccgggag tcgccgaacc cgctgacacg ggccgccatg
     6001 acgacggggg cgttggaacg gccgcgccag gcggcgaaag ccttcctgag ctccgtatag
     6061 acgggattga cctcggacac cgtctcgaca tggcgcgcca gcgacggagc cttcgccgcc
     6121 gcgtcgagga tctcggggag cgagggcggc gaagcgatca ccgccgcatc gccgtaattc
     6181 atgccgatgt cggcggggcg ccgcagcgcc tgcacataat cgaccagcgc gcgggagagc
     6241 atgagctccg cacgggccag ctcggcagga tctcccgagc gcgcccggac catggcttgt
     6301 tcgaggctgg cggcgacggc ggcgcccgcc gtcatcccgt caagctcggc atcgcgaacg
     6361 gcgtcgacca ggcgcgccgc gtcgggcgtg agggcattgc cctttatcca gatagtgcgg
     6421 tgatcccggc cggcatagaa acgcgcgagc gcatgatccg cggaggattg cagcgccgct
     6481 tcgatgaccg gcggagcgac ctgggcgggg gccggcgctg ccgccaccgc gagcagcggg
     6541 agcaaagctg cgatccaacg cctcttgatc ggctgcctgg ccatgctcaa cctctctggt
     6601 ttgcggttcg aagcacaagc ggcgacggcc gcgtcacgcg tgacattggc gggagcggcc
     6661 gttcagccgc atcccatcgt ccgggcctca gccgttatgg ccgccggaat agcggtccat
     6721 cagccgctcg ctgacgaagg taagtccgta ggcgaagccg aagctgatag cggccatgcc
     6781 gagcgcgatc atgtccatct cttcctccgt tcaggccgct tcggcctggt agccatgggg
     6841 acggcgattg tagctgtggc cgccggtggc gccctgcatc accgcttcga cgagcggcag
     6901 agcggtcgcg cagaaggcgc attcggccga cagccggccg acatgccagt ggctccggcc
     6961 gcagccgggg caatggttca cttcccacgg ccggtagacg acgtggtagc cgcgcgcggc
     7021 cgggtcatgg gcgaattgcc tgccgtcttt ggattggatc aacttcaaca ttggtcagcc
     7081 cctttgtccg atgccgccca ttaccacccg gccaccgcca ggcgtgggga ttgcgacggc
     7141 ggccgcacgc gcctcgacgg aaatgcaggg ccggcgacga acgaccccgc ttttccgacg
     7201 aagccggcag acgggccggt tgcgcggccc gcgcgcggcg ctaagccgat cgcacagcag
     7261 gagagggacg aagatgttcg cacgccggga attcatgctc agcaccgccg ctacgggcgc
     7321 gcttgccatc gccggctgcg cgccccgcga cggcgcaatc gcggcggccg gagccgcgcc
     7381 cgctgccggc gatgccgcgg ccgaccgcct gctcgcctcg atcgccgagg agctgctcgc
     7441 cgaatatccc gagaacgcca ccgcgctggg cctcgacaag ggtgagcgcg cgccgctcaa
     7501 ggcgcggctc accgatcgct cgctcgaagg ccgccaccgt ctcgccgccg ccgccgctcg
     7561 ccggctcgag cgcctgcgcg cggtcgaccg cgccggcctc agccccgccg cggcgctcaa
     7621 cgtcgatgtc gtccaggcgg cgcacgagct cgccgccgac ggcttccgct tcccttatgg
     7681 cgacgtggtc gtgctcaacc agcaatggtc gtaccgcaac gcgccttatg tggtggcgca
     7741 gaataccggc gccttcgtcg aaatccccga cttcctcgac agcagccaca aggtcgagac
     7801 cgccgccgac gccgacgcct atctcgcccg gctcgaaggc tatgcggccg cgctcgacgg
     7861 cgagaccgag cgcctccgcc acgacgccgc gctcggcgtg atcgccccga atttcctcct
     7921 cgacaagacg ctgaagcaga tggccggcgc gcgcgcccag ccgatcgcgc aatgggggct
     7981 ggtcacctcg ctcgccggcc gcacccgcga gcttggcggc aattatggcg cgcgcgccga
     8041 gcgcatcgtc gccgaccgcg tcgctcccgc gctcgaccgc cagatcgccg agctcaaggc
     8101 ccatcgcgcg gtcgccaccg acgccgcggg cgtctggaag ctgccgcgcg gcgaggaata
     8161 ttatgcctgg gccctccgcg ccgccaccac cagcaccctc accccggacg aagtccaccg
     8221 caccggcctc gaacagcttc gcgggctgca ggcgcagatg gacgagatcc tccgcgcgca
     8281 aggtctcagc cgcggctcgg tcggcgagcg cctgaccgcg ctcggcaagg acccgcgcca
     8341 cctcttcccc aacacggatg ccggccgcgc tcagatcctc gactatatca acggccgcgt
     8401 cgccgacatc cgccagcgcc tgccgcgcgc ctttgcgacg ctcgtgcccg gcaacctcat
     8461 cgtcaagcgc gtcccgccgg aaatcgaggc cggcgcgccg ggcggctatg cggcggccgg
     8521 aacgatcgac ggcaccgtgc caggcaatta ctatatcaac cttcgcgaca cctcgatgtg
     8581 gccgcgttac tcgctgccca ccctcaccta tcatgagggc atccccggcc acatctggca
     8641 gggcgaatat acctacaagc tgccgctcgt tcgctcgctg ctcgccttca acgcttactc
     8701 cgaaggctgg gcgctttatg ccgagcagct cggcgacgag ctcggcgcct atgacggcga
     8761 accgctcggc cgcctcggct atctgcagtc gatcgccttc cgcgcctgcc gcctcgttgt
     8821 cgacaccggg ctccacacca agcgctggac tcgcgacaag gccattcaat ggttcgcgga
     8881 gaccaacggc tcgtcgatcg aggaagtgtc gggcgaggtc gaccgctatt gcgcctggcc
     8941 cggccaggcc tgcggctaca aggtcggcca tagcgagatc aatcgcctgc gcgacaaggc
     9001 ccgggcggcg ctcggccagc gctgggacct gcgcagcttc gacgacgcgc tcgtgctcgg
     9061 cggcgccgtc ccgctcacct cgctcgaaca ggtgatcgac gcttatattg cgggtcgccg
     9121 cagctgaccc atgttcgagt ctccgctctt gtgagactcg ccgcaatctg ttttcatccc
     9181 gctgcaggcg gctcccgccg gggcggccgc gggggtggcg cttatgagct tcgacaatgc
     9241 tgaggtgaac gcgctgctgc gcgcggtcga gccggccgtc cgcggccagt tcaaggcgca
     9301 gctgcgcccc atcgacttgc gccgccacca cgtcctctac aatctcggca ccgagctcga
     9361 ccgggtctat ttcccgctga gcggcctcgt cggcatccgc gccgagaccg ccgagggcga
     9421 gttcatcgaa agcgccatcg tcggccgcga aggctcgatc ggtgcgttcg aggcgtgcgg
     9481 cagccgccaa tattccgccg aagcggtggt ccaggtgccg ggccgcgcgc tggccatgtc
     9541 cgccgccgcc tatcgcgacc tgttcgacgc ctcgccggcg ctccgcaccg ccgtccaccg
     9601 ctatgtcgag cagttgatga gcgagacccg ccagttcgtc gtctgcaaca cgctccactc
     9661 ggtcgagcag aggctgagcc gtaccgtgct cgaggcgctg gagaagagcg gcctcgacga
     9721 cactttgccc ctcacccagc aggcgctggc gcggatgctc ggcgtgcagc gcaccaccgt
     9781 cgccctcatc ctgtcgcggc tgcagcggga gggcatcacc aacggccgcc gcggcgtcct
     9841 gcgcgtcatt gacctggacg gtctcgagcg gctcgcctgc agctgccgcc tctccctcaa
     9901 ggcaacccgc gaagcgattg cgagcgcgcg gcagccggcc tgcgaagcgg tggtagccgc
     9961 ctgaattcac tccgtttcgg ccgtttgttg ccgagccgac acaaaatccc ggaaagccct
    10021 tgtaagggaa ggctcccgcg gccccggccg ccagtgtctt tgccatgatg cgggccggaa
    10081 tgatcagtct gttctatgtc agccgctcgg ccgtgccgcc ggaccaggaa gccgagcagc
    10141 tcgccggcat cggcgcggtc gcgcagcggc gcaatctcgc ctttggcgtc accggcgcgc
    10201 tggtcctggt cgaccggatc ttcgcccagg tgctggaagg ccccgaagcc gaggtcaacc
    10261 agctgatggt ctcgatcctg cgcgaccggc ggcacagcga cgtccgcatc atcgaaatgg
    10321 cgccgatcga cgagcgccgg ttcgcgacct ggtcgatggc gttcgtgccc ccctccgccg
    10381 ccaccggccg tctgctccgc gctctcgccg acccccgcac cgatctgcgc gccggctcgc
    10441 ccgtcgccga gctactcgac ctgatggccg ccggcgcggg ctccttcacc cgcttccgct
    10501 agccggcgcc gccgcctcgg cccgaatcca ctcctcgaag gcccgcatcg cgcgcgtccg
    10561 ctccttcgat ttcagccggc acagccaata ggacccggtg tcgacctgga cttcgaacgg
    10621 ccgcgccagc gcgccctcgg ccagctcgcg ctcgaacatc gccgccggcg ccagcgccac
    10681 gcccgcgccc tgcagcgccg cctcgatcat cacccaggag gaatcgaaca ccggcccgcg
    10741 caccgccggc gccggaacgc ccgcggcggc gaaccagtcc ggccagtcct ccgcgcgata
    10801 ggagcggagc aggacctcgc gcccgagatc ggccggatcg ccgagccgcg ccgccacttc
    10861 gggagtgcac aaagcggtga gcggcgccgc gaacagccgc atggcttcgg tgccgtgcca
    10921 ggcgccgtcg ccgaagcgga tggcgaggtc gagcccctcg gcggcgatgt cgacccggtt
    10981 attgtgggtc gacaggcgca ggtcgacgaa cggatgggcg gcatgaaatc ggggcagccg
    11041 cggcagcagc cagccgaccg cgaaggtgcc gaccgcgccg acggtgagca cttcgcgcac
    11101 cacgccggcg tcgaaacgct gcagcatctc ggcgatgcgg tcgaacgcat cgcgcagcac
    11161 cggcagcaag gcctggccct cctcggtcag cgccagcccg cgcggcagcc gccggaacag
    11221 cggcgcggcc agccgttgct ccagcccctt cacctgatgg ctgagcgccg ccggcgtcag
    11281 gcacagctcg atcgccgcgc gggtgaagct gagatgccgc gcggccgcct cgaacgcccg
    11341 cagcgcgttg agcggaaggt gaggacggat catcgccgat cccttacttt tcctaatggc
    11401 aagggagagc taacatcgtt tgcggccgcc cgccagccgc gccatcttgc ggctcgacag
    11461 ggagaccggg atgatcgagc gacgcgaatt tacgaggggc ctgtgcgctt cgattggcgc
    11521 ttacgccttg atcgaggcgg tagccggcgc ccgcgcgctg gcgcggccct cgcgcgcggc
    11581 catgagctgg gttgacgccc agcagcagat cgccgccgcg ctcgccgccg gccggatcgc
    11641 cccgcgcgac tggcagcgcg aggtcgaaca gctcgccggc gaggtccatc tgccggagct
    11701 gctcgccgcc accgattatg cccgcctgaa gaccagtttc gacttcaagg gcggcatgcc
    11761 gagcaagcgc ttcgtccgct tcggcgaggg cgccccggcg ctcagctacg gcctcgcctt
    11821 cttcggcttc cgccgcggcc aggcgatcac gccgcacgcg caccgcaaca tggtgtcggc
    11881 gcatatggtg gtggccggcg cgctccgggt gcggaccttc gaccgcctcc gcgacgagcc
    11941 cggcgcgctg atcgtccggc ccaccctcga ccgccggatc gccctcagcg acgtctcgac
    12001 gatgagcagc cagcgcgaca acgtccactg gttcgtcgcc gagcaggatg acagcgccac
    12061 gctcgacgtc atcgtcgacg cgctcgagcc cggccgcgac gcctacacga tcgagctgat
    12121 cgacccgctc ggcggccgcg cgctcggcga cggcagcctg cgcgcgccga tcatcaactg
    12181 ggaggaatcg gtgagcaaat atggccgcgc ctgacctcct cgccgccctc gtcgcggcgc
    12241 tcgcgctcgc cgcgcccgcc gcaccggccc cgggctcgat cgagggcctc agcgcctccg
    12301 acgcgaaggc ggcggccccg gccccgccgt tccggatcgc cgacaatctc tattatgtcg
    12361 gctcgcgcga gaccgccgtc tatctgataa ccggcggcga cggcctcatc ctgctcgacg
    12421 gcgggttcga ggcgatggcg ccgcaggtgc tcgccaacgt cgaggcgctt ggcttcgatc
    12481 ccgcccgcat ccgcctgctg atcaacagcc aggcccattt cgaccatgcc ggcgggctgg
    12541 cggcgatcaa ggcccgcacc ggcgccgcga tggcggcctc cgccgaagat gccgccctgc
    12601 tcgcgcgcgg cggccgcggc gacttcgcct ggggcgacaa gctcccctac cccgcggtca
    12661 aggccgaccg catcgtccag cacggcgaga cggtgaagct cggcgacatc cgcctcaccg
    12721 cccgcctcac ccccggccac agcaagggct gcaccacctg gcagctgcgc accggcgacc
    12781 gcggaaaggc ctatgacgcc ttgttcctgt gcggcaccag cgcgcccggc taccgcctcg
    12841 ccggcaaccc gctttacccg cgcatcgccg aggatttccg gcgctccttc gccacttggc
    12901 gcgcgctgcc gtgcgacatc ttcctcggcg cccacggctc ctattacggc atcgacgcca
    12961 agcgcgccgc cctcgccgcc ggcgccgcgc gcaatccgtt cgtggatccg gagggctgcc
    13021 gagcccatat cgatggggcc gagcagacgt tcgagcgaca attacgggag cagcaagccg
    13081 ccggcccccg ctgatcacca aagggtaggg accgggtacc gccggagagc ccggtccccg
    13141 gtgaccagcg tcagcccatg gtgcagcgcc tgtgcgacca gcatgcgatc gaagggatcg
    13201 ccgtgaaccc gcggcaggcc cgcgagcctc acggggacgt caaaatccat ccccagcgga
    13261 cggaatcctg cgcgatccat cagcgccctc accggctccg gcggcgaaaa ctcggacgag
    13321 cggagccgtt tgacctcgat ctcccaatag cagacgacgc ttacgaagac ttcctgctgt
    13381 gggtcgacca gcgctgcgcg ggctgcgcgg ctcagacgat gatcgtcggc agcgaaccag
    13441 atcagcgcgt gagtgtcagt cagcagcatc gccatccagg tcgggaaaga tcggagcatc
    13501 gtaccattgc gccagctctt cctccgacat gggtgcgaac caatcgatgt cgtcgttgat
    13561 cctgcccttc cagctcccca gcgccttgcg ccgcttttcg gccagatcgc gaagatgctg
    13621 gtccggatcg accggtacca atcgcgcttc gggagacccg gctcgcgtga tgatcacttc
    13681 ctcgccgcgc ttggcggcgg cgagcagctc cgacaagcgg gtcttggcgg cgccgatgtt
    13741 aacgtggatc gtcatggctt ggccaagata gcttggccaa ggttccggat caagggttac
    13801 tcattcccac tcgatggtcc ccggcggctt cgaggtataa tcgtagacca ccctgttgat
    13861 gccgcgcact tcgttgatga tgcgggtggc gacgcggctc aggaaggtgg cgtcgaacgg
    13921 gtagatgtcg gcggtcatgc cgtcggtcga ggtcaccgcg cgcaaggcgc agacgctgtc
    13981 ataggtgcga tgatcgccca tcactccgac cgtgcgcacg ggcagcagca ccgcgaacgc
    14041 ctgccagatg gcgtcataga ggccggccat gcggatctcg tcgagataga tggcgtcggc
    14101 cttgcgcagg atgtcgcatt tctccttggt gacttcgccg gggatgcgga tggcgagccc
    14161 ggggccgggg aagggatgcc gtccgacgaa cgcctccggc aggccgagct cgcggccgag
    14221 cacgcgcacc tcgtccttga acaattcgcg cagcggctcg accagcttca tgttcatccg
    14281 ctcgggcagg ccgccgacat tgtggtggct cttgatcgtc accgacgggc cgccggtgaa
    14341 gctgacgctt tcgatcacgt ccggatagag cgtgccctgg gcgaggaagt cggcgccgcc
    14401 gatcctgccc gcttcctcct cgaacacttc gacgaaggtc ttgccgatga acttgcgctt
    14461 ggcctcggga tcgtcgagcc cggcgagccc ggacaggaac aagtcctcgg cgtccacgtg
    14521 caccagcggg atgttgtagg aattgcggaa caggctcacc acctgctccg cctcgccgag
    14581 ccgcatcagg ccatggtcga cgaacacgca ggtgagctgg tcgccgatcg cctcgtggat
    14641 cagcaccgcc gccaccgcgc tgtcgacgcc gcccgaaagg ccgcagatca cccggcccga
    14701 accgacctga gcgcggatct cggcgatctt ggcctcgcgg aactcggcca tggtccagtc
    14761 gccggcgagc ccgcagacat ggcgcacgaa attggcgatg agcttgccgc cgtcgggagt
    14821 gtgcacgacc tcggggtgga acatcaggct gtagaagcgc cgctcctcgt cgaccgccac
    14881 cgcataaggc gcgccttccg actgggcgac gaccttgaag ccgtcgggca ggcgctcgac
    14941 gcggtcgcca tggctcatcc acacctggtg gcgctcgccg ggggaccaca gtccgtcgaa
    15001 cagcgacgac tggccggtaa tgtcgatgaa ggcgcggccg aactcgcgat tgtcggacgg
    15061 cgcgaccgag ccgccgagct gctgcgccat ggtctgctgg ccgtagcaga tgcccatcac
    15121 cggcagcccc gcctcgagca gctgctgcgg agcgcgcggg ctgtcctccc aggtcaccga
    15181 cgatggcccg cccgagaaga tgatcccctt gggcttcagc cgctcgaacg cctcggcggc
    15241 cgcgttgaac ggggcgatct cgctatagac ccccgcctcg cgcacccggc gggcgatgag
    15301 ctgggtcacc tggctcccga aatcgacgat gaggatggtg tcggccgccg ggctggctgg
    15361 ggtctggtct gtcatggcag gccgataagg ccgaactccc gcgtctgtcc agtcgcggcg
    15421 ggctccggcg tctggttaac cgcgccttag tttattgatc ctagcgtcgc ggctgcgaac
    15481 ggaggggatg aaatgccgag acggatcaag gcgctgctcg accagctcga aagcgaggtc
    15541 ggccgcttcg cccaggccaa tgagcgcatc gccaagcaga ccaatttcct ggccctgaac
    15601 gccaccatcg aggcggcgcg ctcgggcgat gccggccgcg gcttcgcggt cgtcgcgcag
    15661 gaggtgaagg cgctggcggc ccaggcccgc gccgcctcca acgattttcg tgcgctcgtc
    15721 atcgaccggc tcaagactgg ctcgaccatc gccgacagcc tggtcgacga catcgagggc
    15781 agccggctcg ccgaattcgc cttcgccacc gtccagacct tcgcccgcaa catctattcg
    15841 cgcagcagcg acatccgcat cctcgccacc gacagcgaca tcgtcgccgc cgcgcgcgcg
    15901 ccggacgacc gcgccctcat cgcccgcgcc gaggatcgcc tgcgcgccgc gatgcgcttc
    15961 tcgccccatt atgtgaacgc ggtcttcgcc acccgcgccg gcaaggtggt cggctcggcc
    16021 gatccgcgca gccccctgct cggcgtcgac gtcggagccg aaccgcagtt caacaacgtt
    16081 atccagagcc gctccaccga cgactggttc accgacgccg tgtgggagaa tccctattcg
    16141 aacggccgcc aggtgctgat cttcgcctgc ggcatccgcg cgctcgaaca tgacgggcag
    16201 ccggccgaag gctgcctcta cctcgaatat gattggcagg gtaacgccgc cgaacagctc
    16261 caggcgcagc tcgccgccgc cgataaggag atacgcagcg ccaccctccg catcatggag
    16321 catggcggcc gcatcgtcgc ctcgaccctg ccgggcgagt tcgacaaaca ttatgcgctg
    16381 cctgagaacc gccccgcccg cggcatgatc tccaagggcg accgcgtcgt cgcttatgcc
    16441 gacgccaacg accgcggcgg ccgcaattcg ctcaacctca cctgcgtgat cgagcatttc
    16501 atcgaaagcg gcggcgaggc cgcgatcgcc gcctgaggcc cttcgctcag ccgcccatat
    16561 aggcggtgaa atcggcgttg gtgagcggcg tgttcggatt caccttggca aacaatatgg
    16621 cgccggcgcc gccgatgccg tcgctgtcgt agaagatgct gccgctaccc gcatcgtaga
    16681 cgatccggtc ggtacggtcc tgcgcggagg tgcccaggcc gaagccgctg gcggcgagct
    16741 ggccgtccac ggcgatcccg gcgaacaccg tccggtcgag caggatcgtg tcctgcggcg
    16801 ccgaaaagtc agtgatcgta tcgacgttcg agaccgggtt gagaagggtg ttgaaatcga
    16861 acctgtcggc gcccccgccg ccggtcagct ggtccgcgcc ccggccgccc gccagcacat
    16921 cgttgccgcc gc
//
LOCUS       DASVRK010000120         8383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_581642, whole genome shotgun sequence.
ACCESSION   DASVRK010000120 DASVRK010000000
VERSION     DASVRK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581642"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3458
                     /locus_tag="VF589_04590"
     CDS             <1..3458
                     /locus_tag="VF589_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8256887.1"
                     /translation="TAVARGDLSKKITVDVKGEILALKDTINVMVDQLNGFASEVTRV
                     AREVGTEGKLGGQAQVPGVGGTWKDLTDNVNLMATNLTNQVRGIADVVTAVAQGNLKR
                     KLQVDAKGEIAALAETINFMIDTLSTFGDQVTNMAREVGIEGRLGGQAKVPGAAGLWR
                     DLTDNVNQLAANLTNQVRSIADVATAVTKGDLTRSIAVEASGEMAALKDNINEMIRNL
                     KEQTLKNAEQDWLKTNLARFSRMLQGERDLATVSNLIMSELAPLVNAQYGVFYVTKRE
                     EDETVLELAASYGAESRDQLKPVFKLREGLVGQAAADKRGILLENIPGEFIRIGSGLG
                     HAPPANINIMPALFEDDVKAVIELASFSEFNETHQSFLDQLMESVGIVLNTIAATMRT
                     EGLLKQSQLLTQELQARQTELTTKQEELHATNEELQEKAQLLENEKKQVEAKNFEIEM
                     ARRAVEEKAEQLALTSKYKSEFLANMSHELRTPLNSLLILSKLLADNPQGNLNEKQTE
                     FARTIHSAGSDLLSLINDILDLSKIESGTVSIEVGDMPMSSLRQHMERTFRQLAADKG
                     LVFDVEFDPALPPAIRTDEKRLQQVVLNLLSNAFKFTSQGGVTLAVRCVGGGWSTNHP
                     VLKNADRAIAIAVTDTGIGIPEDKQKLIFEAFQQADGTTSRKFGGTGLGLSISREIAR
                     LLGGELQVRSRPGEGSTFTLFVPLQAGAAAPAATTSAQPRYDNSGAMVPSALPAGAEV
                     VDDRDNLGTDAFVLIVEDDVTFASILLDLARDAGLKGVISTAGAGTLALARKLQPDAI
                     TLDLGLSDIDGFVLLDLLKHDPQTRHLPIHVISGADEVASVLGMGAFGVTAKPADHEE
                     LSQVFASIHGHIQRAERRVLVIGGDAADRKAIGAAIADPLAEVQSAASLAKSKAKLNL
                     AQFDVVVVAGGEKPEASREIIALADGGGNAAVLVVCKGDEHHATSKALHRLVDTHDVS
                     VVHSLEGLPAALGPVLRRGSGPINDNDAPVRTREVPELAGAKILIVDDDIRNIYSLTS
                     VLESYDVEVLHAERGRDGILILEQTPGIDIALIDIMMPEMDGYETMQQIRARPALANM
                     PLVAVTAKAMRGDRQKCLDAGASDYIAKPVDIELLLALLRVWVQRARGRAAAGRPALT
                     QTAAE"
     gene            3493..5550
                     /locus_tag="VF589_04595"
     CDS             3493..5550
                     /locus_tag="VF589_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8256888.1"
                     /translation="MAAAAPLKALEEVAPDVERARVLVVDDDERNLLAIQTVIEDEAD
                     VVVARSGEEALRHLLKGEFAVILLDVYMPGMDGYETAQIIRSREQTKRIPIVFLSAVN
                     KETEHLIRGYSMGAVDYVFKPVEPVVLRSKVAVFVDLFQMTREIQRKARQEQALLDAN
                     LRANAERLRVEQELRLAQQRQAAIIESLPIILYLEPLDVSPRVPAFVSGNFEAVTGYD
                     FDHVLKHPNLWADQLHDDDRERVLAALAARTETGAFSVEYRWRCADGNYKHFLDQTVL
                     LRDANGTPVEFAGTLMDVTDRKLLENQLIHSQKMDAVGKLTGGIAHDFNNLLAAVLGG
                     LGLIERRVALTEDQRKIMTMTRHAAEQGSELVGRLLAFARRQKLEPARIELSKLSATV
                     TDLLAHTLGGLVQLHWQIDDDLWRAYADETQLELGLMNLIINARDAMPDGGTIRVTGS
                     NVEVNAEGELGVPPGQYVVLAVTDQGCGIAPDILEQVMEPFFTTKDVGKGTGLGLSMV
                     YGFAKQSGGAIRIDSKVGEGTRVEIWLPRAPAALPGEAAARSAEGVAEPLTPAGPLSI
                     LLVDDHAGVRATTAAMLEDLGHEVSQSASGAELLATLAEDPDGFDVIVSDYAMPLVSG
                     ADVIRRAREIVPGMPGIIITGYADIQSISKRPEDVTVLTKPFTPDQLAAALRAIALRD
                     RAA"
     gene            complement(5694..6587)
                     /locus_tag="VF589_04600"
     CDS             complement(5694..6587)
                     /locus_tag="VF589_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8256889.1"
                     /translation="MIDLVIEPRRKDLGGFEVGRVLPYARRRMVGPFVFFDHMGPADF
                     APGLPRSADVRPHPHIGLSTVTYLLTGEIIHRDSLGIEQAIRPAEVNWMTAGSGITHS
                     ERFEKGRAVGDRLHGLQAWVALPEADEETDPSFSHHEAADLPLIEDAGVTARIIVGKA
                     YGQAARVPTFSPIFYVHLDLADGARAEVPDEYSERAAYIVEGVVAVDGQRFADGRMIV
                     FAPGQPATITAEGPATVMLLGGEPVGPRYIEWNFVSSSKERIEQAKADWRAGRMKLPD
                     LDHDEFIPLPEDPVPPANPMS"
     gene            complement(6584..6898)
                     /locus_tag="VF589_04605"
     CDS             complement(6584..6898)
                     /locus_tag="VF589_04605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8256890.1"
                     /translation="MASEVVHNREEQRFELKQGDEVAYAEYEREGETIIFTHTVVPPA
                     LEGHGIGTELVRAGLAAVREAGGKVVPACPFVARYIERHPEDRELLDPAYVHSQQGLE
                     NI"
     gene            7042..7902
                     /gene="nadC"
                     /locus_tag="VF589_04610"
     CDS             7042..7902
                     /gene="nadC"
                     /locus_tag="VF589_04610"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX8256891.1"
                     /translation="MAETFVAGFDLGAFVRAVLAEDLGEGGDVTSAATIPADARLEAV
                     MATREPVTVAGLALAASFFRALDPAVMAEPLVEEGALVPAGAELMHLSGNARAMLAAE
                     RSALNSLQHLTGIATITRAYADAIAGTGCILLDTRKTIPGLRVLEKYATRMGGAQNHR
                     MRLDDGVLIKDNHVAVAGGVAAAVRAAKAAGLTLPVQVEVDQIEQIEPALAAGADRLL
                     LDNMPPAILRKAVALIAGRVPTEASGGVRLDTIRAIAETGVTYVSVGRITQSAPAADI
                     GLDYRRPRAQ"
     gene            8013..>8383
                     /locus_tag="VF589_04615"
     CDS             8013..>8383
                     /locus_tag="VF589_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256892.1"
                     /translation="MATNGNDTVNGTTGNDILKGLGGDDILNGLDGNDTLNGGAGIDA
                     MRGGNGNDIYIVDTIGDKAIETSANTHTPGGRDLVKSSINFTLGAFVEDLLLFGDVAV
                     KATGNDLNNRLTGNDIGNILDG"
BASE COUNT         1476 a         2924 c         2725 g         1258 t
ORIGIN      
        1 cgaccgccgt ggctcgcggc gatttgtcca agaagatcac tgtcgacgtg aagggcgaaa
       61 tccttgccct caaggacacg atcaacgtca tggtcgatca gttgaacggc ttcgcttccg
      121 aagtgacccg cgttgcgcgc gaagtcggta ccgagggcaa attgggcggc caggcgcagg
      181 tgcccggcgt gggcggcacg tggaaggacc tcacggacaa cgtcaacctg atggcgacca
      241 acctcaccaa ccaggtgcgc ggcatcgccg acgtggtgac cgcggttgcg cagggcaacc
      301 tcaagcgcaa gctgcaggtc gacgccaagg gcgagatcgc ggcgctggcg gagacgatca
      361 acttcatgat cgacacgctt tcgaccttcg gcgaccaggt gaccaacatg gcccgcgaag
      421 tcggtatcga agggcgtctg ggcggacagg ccaaggtgcc gggcgccgcc ggcctgtggc
      481 gcgaccttac cgacaacgtg aaccagctgg ccgcgaacct cacgaaccag gtgcggtcga
      541 tcgccgacgt ggcgaccgcc gtgaccaagg gcgacctcac ccgctcgatc gcggtggaag
      601 cctcgggcga gatggcggcg ctgaaagaca acatcaacga gatgatccgc aacttgaagg
      661 agcagaccct caagaatgcg gagcaggatt ggctcaagac caacctcgcc cgcttctcgc
      721 ggatgctgca gggcgagcgc gacctcgcca ccgtctcgaa cctcatcatg tcggagctcg
      781 cgccgctggt gaacgcgcaa tacggcgtgt tctacgtgac caagcgcgag gaggacgaga
      841 ccgtgctcga gctcgccgcc agctacggcg ccgaaagccg cgaccagctc aagcccgtct
      901 tcaagctgcg cgaagggctg gtcggccagg ccgccgccga caagcgcggc atattgctcg
      961 agaacatccc gggcgagttc atccgcatcg gctctggcct cggccacgcg ccgccggcca
     1021 acatcaacat catgccggcc ttgttcgagg acgacgtcaa ggcggtgatc gagctcgcct
     1081 cgttcagcga gttcaacgag acccaccaga gcttcctcga ccagctgatg gaatcggtcg
     1141 gcatcgtgct caacaccatc gccgccacca tgcgcaccga gggcctcttg aagcagtcgc
     1201 agctgctcac ccaggagctg caggcgcgcc agaccgagct caccaccaag caggaagagc
     1261 tgcacgccac caacgaggag ctgcaggaga aagcgcagct gctcgagaat gagaagaagc
     1321 aggtcgaggc gaagaatttc gagatcgaga tggcccgccg cgcggtcgag gagaaggccg
     1381 agcagctggc gctcacctcc aaatacaagt cggaattcct cgccaacatg tcccacgagc
     1441 tgcggacgcc cttgaactcg ctgctgatcc tgtcgaagct gctggccgac aatccgcagg
     1501 gcaacctcaa cgagaagcag accgagttcg cccgcaccat ccactcggcg ggctcggacc
     1561 tgctgtcgct gatcaacgac atcctcgacc tttccaagat cgaatcgggc acggtgtcga
     1621 tcgaggtcgg cgacatgccg atgtcgagcc tgcgccagca tatggagcgc accttccgcc
     1681 agctcgccgc cgacaagggg ctggtgttcg acgtcgagtt cgatcccgcg ctgccgccgg
     1741 cgatccgcac cgacgaaaaa aggctccagc aggtcgtgct caacctcctg tcaaacgctt
     1801 tcaaattcac gtcgcagggc ggcgtcacgc tggcggtgcg ctgcgtcggc ggcggctgga
     1861 gcaccaacca tccggtgctg aagaatgccg accgggcgat cgccatcgcc gtcaccgaca
     1921 ccggcatcgg catccccgag gacaagcaga aactgatctt cgaggcgttc cagcaggccg
     1981 acggcaccac cagccgcaaa tttggcggca ccggcctcgg cctgtcgatc agccgcgaga
     2041 ttgcgcgcct gctcggcggc gagctccagg ttcggtcgcg gccgggcgaa ggctcgacct
     2101 tcaccttgtt cgtcccgctc caggccggcg ctgccgcgcc cgccgcgacg acctcggccc
     2161 agccacgcta cgacaatagc ggcgcgatgg tgccgagcgc gcttccggcc ggtgccgaag
     2221 tagtcgacga ccgcgacaat ctcggcaccg atgcgttcgt gctgatcgtc gaggatgacg
     2281 tcaccttcgc ctcgatcctg ctcgatctcg ctcgcgacgc cggcctcaag ggggtgatct
     2341 ccaccgccgg cgccggcacg ctggcgctgg cgcgcaagct ccagcccgac gcgatcacgc
     2401 tcgatctcgg cctcagcgac attgacggat tcgtgctgct cgatctgctg aagcacgacc
     2461 cccaaacccg gcaccttccg atccacgtca tctcgggcgc cgacgaagtc gcttcggtgc
     2521 tcggcatggg cgcgttcggg gtcactgcga agccggccga ccatgaggag ctgagccagg
     2581 tattcgcctc gatccacggc cacatccaac gcgccgagcg gcgggtgctg gtgatcggcg
     2641 gcgatgcggc ggaccgcaag gcgatcggcg ccgccatcgc cgacccgctc gccgaggtgc
     2701 agagcgcggc cagcctcgcc aagtccaagg ccaagctcaa tctcgcccag ttcgacgtgg
     2761 tggtcgtcgc cggcggcgag aagccggagg cgagccgcga gatcatcgcg ctggctgacg
     2821 gcggcggcaa cgcggcggtg ctggtggtct gcaagggcga cgagcatcat gcgaccagca
     2881 aggcgctcca ccggctcgtt gacactcacg atgtatcggt cgtgcacagc ctcgaaggcc
     2941 tgccggcggc gctcggcccg gtgcttcggc gcggcagcgg cccgatcaac gacaatgacg
     3001 ccccggtccg cacccgcgag gtgccggagc tggccggcgc caagatcctg atcgtggacg
     3061 acgacatccg caacatctac tcgctgacca gcgtgctcga gagctatgac gttgaagtgc
     3121 tccacgccga gcgcggccgc gacggcatcc tgatcctcga acagacgccg ggcatcgaca
     3181 tcgcgctgat cgacatcatg atgccggaga tggacggcta cgagaccatg cagcagatcc
     3241 gcgcccgtcc ggcgctcgcc aacatgccgc tcgtggccgt caccgccaag gcgatgcgcg
     3301 gcgaccgcca gaagtgcctc gacgcaggcg cgtcggatta catcgccaag ccggttgaca
     3361 tcgagctgct gctcgcgctc ctgcgggtct gggtgcagcg tgcgcggggc cgggcggcgg
     3421 ccgggcggcc ggcgctgacg cagacggcgg cggaatgaac gggtgagcat acaacagccg
     3481 atgaggaggg gcatggccgc ggcggccccg ctgaaggcgc tggaggaggt ggcgcccgac
     3541 gtcgagcggg cgcgcgtgct cgtcgtcgac gatgacgagc gcaatctgct cgccatccag
     3601 acggtgatcg aggacgaggc cgacgtggtc gtcgcccgct cgggcgagga ggcgctgcgc
     3661 cacctgctca agggcgagtt cgcggtaatc ctgctcgacg tctacatgcc gggcatggac
     3721 ggctacgaaa ccgcccagat catccgctcg cgcgagcaga ccaagcgcat cccgatcgtc
     3781 ttcctgtcgg cggtcaacaa ggagaccgag catctgatcc gcggctattc gatgggtgcg
     3841 gtcgattacg tgttcaagcc ggtcgagccg gtcgtcctgc gctcgaaggt cgccgtgttc
     3901 gtcgacctgt tccagatgac gcgcgagatc cagcgcaagg cgcggcagga gcaggctttg
     3961 ctcgacgcca atttgcgcgc caatgccgag cggctgcggg tcgagcagga gcttcggctc
     4021 gctcagcagc gccaggcggc gatcatcgaa tcgctgccga tcatcctcta tctcgagccg
     4081 ctcgacgtca gcccgcgcgt acccgccttc gtcagcggca atttcgaggc ggtgaccggc
     4141 tatgatttcg atcacgtcct gaagcacccc aacctgtggg ccgatcagct ccacgacgac
     4201 gaccgcgagc gcgtgctcgc cgcgctcgcc gcccgcaccg agaccggcgc cttctcggtc
     4261 gaatatcgct ggcgctgcgc cgacggcaac tacaagcatt tcctggacca gaccgtgctc
     4321 ttgagggacg cgaacggcac tcccgtcgag ttcgccggca cgctgatgga cgtcaccgac
     4381 cgcaagctgc tcgagaacca gctgatccac agtcagaaga tggacgcggt gggcaagctc
     4441 accggcggca tcgcgcacga tttcaacaat ctgctcgccg ccgtgctcgg cgggctcggc
     4501 ctgatcgagc gccgcgtcgc gctgaccgag gaccagcgca agatcatgac catgacgcgc
     4561 catgccgccg agcagggctc ggagctggtc ggccggctgc tcgccttcgc ccgccgccag
     4621 aagctcgaac cggcgcggat cgagctttcc aagctttccg ccaccgtcac cgacctgctc
     4681 gcgcacacgc tcggcggcct cgtccagctc cactggcaga tcgacgacga cctgtggcgc
     4741 gcttatgccg acgaaaccca gctcgagctg gggctgatga acctcatcat caacgcccgc
     4801 gacgccatgc ccgacggcgg cacgatccga gtgaccggca gcaatgtcga ggtcaatgcc
     4861 gagggcgagc tcggggtgcc gccaggccaa tatgtcgtgc tcgccgtcac cgaccagggc
     4921 tgcggcatcg cccccgacat actcgaacag gtgatggagc cgttcttcac caccaaggat
     4981 gtcggcaagg gcaccgggct cggcctctcg atggtctacg gtttcgccaa gcaatcgggc
     5041 ggcgcgatcc gcatcgacag caaggtgggc gagggcaccc gggtcgagat ctggctgccg
     5101 cgcgcaccgg cggcattgcc gggcgaggcc gccgcgagat ccgccgaggg agttgccgag
     5161 ccgctaacgc cggccggccc gcttagcatc ctgctggtcg acgaccatgc cggagtgcgc
     5221 gcgaccacgg cggcgatgct cgaggatctc gggcatgagg tcagccagtc ggccagcggc
     5281 gccgaattgc tagcgacgct cgccgaggac cccgacggct tcgacgtcat cgtcagcgat
     5341 tatgcgatgc cgctcgtgtc cggcgccgac gtcatccgcc gcgcgcgcga gatcgtcccc
     5401 ggcatgcccg gcatcatcat caccggctat gccgacatcc agtcgatctc gaagcgcccc
     5461 gaagacgtca ccgtcctcac caagcccttc acccccgacc agctcgccgc cgccctccgc
     5521 gccatcgcgc tgcgcgacag agctgcctga aggtcaagca ccggcggggg tgacgtcatt
     5581 ccagcgaaag ctggaatctc ccttcttctt tcccggctcc caaagaagtg agatgccagc
     5641 tttcgctggc atgacgacgc tctctcgctg gcatgacgct tcagccgacc cgctcacgac
     5701 atcgggttgg cgggcgggac cggatcctcg gggagcggga tgaactcgtc atggtcgagg
     5761 tcggggagct tcatgcggcc ggcgcgccag tcggccttgg cctgctcgat ccgctccttg
     5821 gacgacgaca cgaaattcca ctcgatatag cgcggcccga ccggctcgcc gccgagcagc
     5881 atcaccgtcg ccggcccctc ggccgtgatc gtcgccggct ggcccggcgc gaacacgatc
     5941 atgcggccgt cggcgaaccg ctggccgtcg accgcgacga ccccttcgac gatataggcg
     6001 gcgcgctcgc tatactcgtc cggaacctcc gcccgcgccc cgtcggcaag atcgaggtgg
     6061 acgtagaaga tcggcgagaa ggtcggcacc cgcgccgcct ggccataagc cttgccgacg
     6121 atgatccgcg ccgtaacgcc cgcatcctcg atcagcggca ggtcggccgc ttcatggtgg
     6181 ctgaagctgg ggtcggtctc ctcgtcggct tcgggcagcg ccacccaggc ctgcagcccg
     6241 tgcagccggt cgccgaccgc gcggcctttc tcgaaccgct cgctatgggt gatgccgctg
     6301 ccggcggtca tccagttgac ctcggccggg cggatcgcct gttcgatgcc gaggctgtcg
     6361 cggtggatga tctcgccggt cagcaaatag gtgacggtcg acaggccgat atgggggtgc
     6421 ggccgcacgt cggcggagcg cggcaggccc ggcgcgaagt cggccggtcc catatggtcg
     6481 aagaagacga atggccccac catccgccgc cgcgcatagg gcagcacccg cccgacctcg
     6541 aacccgccca ggtccttgcg ccgcggctcg atgacgaggt cgatcatata ttctccaatc
     6601 cctgttgcga atggacatag gccggatcga gcagctcgcg gtcctccgga tggcgctcga
     6661 tatagcgggc gacgaacggg caggccggca ccaccttgcc gcccgcctcg cgcaccgcgg
     6721 cgaggccggc gcgcaccagc tcggtgccga tgccatggcc ttcgagcgcc ggcggcacga
     6781 ccgtgtgggt gaagatgatg gtctcgccct cgcgctcata ttccgcataa gcgacctcgt
     6841 cgccctgctt cagctcgaag cgctgctctt cgcgattgtg caccacttcg ctggccatcg
     6901 acgcctccgt tcgcccgccc ggccgcacta atgccgcgcc gggcgccgcc gtcaagcgcc
     6961 taggcggcga tcgggggttc gcggcggcgg agcgggctgg taggcgcgtc gcgtcccgat
     7021 ttcaccgccg gagctgcaaa catggccgaa actttcgtcg ccggattcga tctcggcgcc
     7081 ttcgtccgcg ccgtcttggc cgaggatctg ggggaggggg gcgacgtcac ctccgccgct
     7141 accatcccgg ccgatgcccg gctcgaggcg gtgatggcga cgcgcgagcc ggtgacggta
     7201 gccggcctcg cgctggccgc ttcctttttc cgcgcgctcg acccggcggt catggccgag
     7261 ccgctggtgg aggagggcgc cttggtgccg gccggagccg agctgatgca cctctccggc
     7321 aacgcccgcg caatgctggc ggccgagcgg tcggcattga acagcctcca gcatctcacc
     7381 ggcatcgcca ccataacccg cgcttatgcc gacgccatcg ctggcaccgg ctgcatcttg
     7441 ctcgacacgc gcaagactat cccgggcctt cgcgtgctcg aaaaatatgc cacccgcatg
     7501 ggcggcgcgc agaaccaccg catgcgtctc gacgacggtg tgctgatcaa ggacaatcac
     7561 gtcgccgtcg ctggcggcgt ggccgcggcg gtgcgcgccg ccaaggccgc ggggctcacg
     7621 ctccccgtcc aggtcgaggt cgaccagatc gagcagatcg agcccgcgct cgccgccggc
     7681 gcggatcggc tgctgctcga caacatgccg ccggcgatcc tgcgcaaggc ggtcgctctg
     7741 atcgccggcc gcgtgcccac cgaagcgtcg ggcggggtcc ggctcgacac catccgcgcc
     7801 atcgccgaaa ccggggtcac ctatgtctcc gtcggccgca tcacccagtc ggcgcccgcc
     7861 gccgatatcg gcctcgacta tcgccggcct cgcgcgcaat gaaaaagatg gttgattatt
     7921 agaggtttat tgaccgccga ctatcggcgg cgtaaatcaa ctctcatcgg gaaggcgcga
     7981 ctccgggcgc ctggggacag tgggggggcg aaatggctac caacggcaac gacacggtta
     8041 acggcaccac cggcaacgac atcttgaagg gcctgggcgg cgacgacatt ctgaacggcc
     8101 tcgacggcaa cgacactttg aacggcggcg ccggcatcga cgccatgcgc ggcggcaacg
     8161 gcaacgacat ctacatcgtt gacacgatcg gcgacaaggc gatcgagacc agcgccaaca
     8221 cccacacgcc gggcggccgc gacctcgtca agagcagcat caacttcacg ctcggcgcgt
     8281 tcgtcgagga tctgctcctg ttcggcgacg tcgccgtcaa ggcgaccggc aacgatctca
     8341 acaaccgcct caccggcaac gacatcggca acatcctcga cgg
//
LOCUS       DASVRK010000121        11715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_519530, whole genome shotgun sequence.
ACCESSION   DASVRK010000121 DASVRK010000000
VERSION     DASVRK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519530"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..499)
                     /locus_tag="VF589_04620"
     CDS             complement(86..499)
                     /locus_tag="VF589_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEX8256893.1"
                     /translation="MRLTSLADYAVVMMAAAARHGGEARLSAGLLAEETGVPLPTAQK
                     LMGRLAAAGLLSSARGTGGGFRLAREAQGISLADIIEAVEGPIAMTVCVDEKRNDCAL
                     DGHCLVKPHLNVVNGAVRGALQGVSLATLAQGEVK"
     gene            complement(707..2569)
                     /locus_tag="VF589_04625"
     CDS             complement(707..2569)
                     /locus_tag="VF589_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8256894.1"
                     /translation="MIRIALLASTALLAACAATPAAPPVESASAAPPAPAAAPVAAPA
                     QDSAELAAFFDAYDKAALELSPLGKSFRGIVDEDYGAWGDFSEAGAQREQELDRTSLA
                     AMKTQFDRDALSPADRLSYDLFENMVTRGERAFEFRRNSYTFDQMNGAQSQLPAFLIN
                     IHRVTDVPTAEAYVSRLAAVDTVIDQVLAESRERAALGVLPPKWVYPYVINDARNVIK
                     GAPFTSGADSALFADFKKKVDALTVAAPEKTRLIAAGRQALLNDVKPAYQRVITEMER
                     QQKLAGTDDGIWRLPRGAEYYAERLGYYTTTGMSPAEVHDLGLRQVARIHDEMREVQQ
                     RIGFKGSLQDFFVHMRKRPQNYFANTPAGRQEYLDETQKAIDAITARVPEFFSPATTP
                     KAPLTVKAVEPFREKSAGKAFYQDPAPDGSRPGVYYVNLYAMGDMPNTEVEALAYHEG
                     IPGHHLDGAVTTRLENLPAFRRFGGYTAWSEGWGLYAERLAKDMGMYQDPYRDFGRLQ
                     LELHRAIRLVVDTGLHDKRWTRERAIKYVEDNSADAPGGVVKAIERYVIFPGQATAYM
                     VGRLKIMELREWARGELGGRFDIKGFHDAVLLSGAVPLDIMERNVRDWVARTKA"
     gene            2666..3730
                     /locus_tag="VF589_04630"
     CDS             2666..3730
                     /locus_tag="VF589_04630"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX8256895.1"
                     /translation="MALYPLLRPLVFALDAEQAHRLTLRALKLKPAGRPPERDARLGI
                     RVAGLNFPNPLGLAAGFDKDAEVFEQILGLGFGFAEVGTLTPLPQAGNPKPRLFRLAE
                     DRAVINRMGFNNEGQQAALQRLQARKRVSPSRRRPTEAKAGIVGVNIGANKDSADRIA
                     DYSAGVAAMTGVADYLTVNISSPNTPGLRDLQSGSALDELLAAIAEVRGAGPPIFVKV
                     APDLDPAQIDAIVAAAVARKMDGIIVANTTIGRPDLRSRFRDEAGGLSGAPLKALALE
                     RLRDFRRASGGRLPLIAAGGIENGVDAYARIRAGASLVQVYSALVYRGPGLARHILGE
                     LRTLLRRDGFRTLAEAVGTE"
     gene            3846..5558
                     /gene="ggt"
                     /locus_tag="VF589_04635"
     CDS             3846..5558
                     /gene="ggt"
                     /locus_tag="VF589_04635"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8256896.1"
                     /translation="MTCLNPLRILLPLLLLAACAIQPVNGAPVETAARAGGVVSAADP
                     RAAEAGRYILNAGGSAADAAVATMLALTVVEPQSSGIGGGSFLVYHDEAADRISTYDG
                     REEAPAAATPRLFLGPNGEPLPFGEAMPGGKSVGVPGNLRVAAMIHERHGKLPWARLF
                     EPAIRLASQGFAITPRMHGMLGGRAGHAGMTAWARAQYFGADGPKPVGTIVRNPELAR
                     LYETIAARGADYFYTGPPAQNLVRTVTTAARNPSSMTLGDLASYDAKERPVLCGEYRR
                     HRICGMGPPSSGATTVFAILKQLERFDMAAMRPSDPAAWHLIAESMRLAYADREAYIG
                     DPDFVRVPSAGLMDPAYLAARSRLISPARAMRSAAAGTPPGAPRVTAAADGEVPSTSH
                     FVAVDAAGNVASMTSTIEGVWGSGLTVDGYYLNNELTDFSFAPEIDGAPVANRVEGGK
                     RPRSSMSPTIVYGPDGEVRLAVGAAGGPTIIAQVAKAIIGVVDWKLSAQEALALPQIV
                     AIGDTVRVERGTALEPMIPALTALGHRAVAADPGFKANAAERVGGRWRGAADPRSEGV
                     ALAQ"
     gene            5676..7466
                     /locus_tag="VF589_04640"
     CDS             5676..7466
                     /locus_tag="VF589_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8256897.1"
                     /translation="MQAFEQFPNLVTMFFARAAEKGEAPFLWAKKEGQWRSTSWREAA
                     ETVAALAAGLKRIGLGRGDTVMLVSENRPEWCIADLAIMAAGCVTVPAYVTNTQRDHQ
                     HILDDSGAAAVIVSSAKLAKPLLPAVLRANSCEHVIAIDPIHAGQSGTVQYHDWHKLI
                     AAEAPDVAATAAAATFGRGDLACIIYTSGTGGAPRGVMQHHGAILHNVEGCIDVIAED
                     FGLDEEVFLSFLPASHAYEHSGGQFFPIGIGGQIYYSEGLEKLASNIEEVRPTIMVVV
                     PRLFEVLRQRIMKNVEKQGKITNYLLRRALDIGAKDYAGQVPLWDRPMDFILGRTLRP
                     KVSARFGGRIKAMVSGGAPLNPEVGIFFHSMGLTLLQGYGQTEAAPVISCNRPRAGIK
                     MDTVGPPLKNTEVRIAEDGEILVRGELVMKGYWRNPALTERTIVDGWLHTGDVGHIDA
                     KGRIVITDRKKDLIINDKGDNVAPQKIEGMLTLQPEIVQAMVAGDKKPYVVGLVVPDP
                     EWTGEWAKAAGKPADAAALRDDSDYLKAVSAAVDRVNAELSVIEKVRRFILADEPFTT
                     DNEQLTPSIKIRRHVLRQVYGERLDALYRR"
     gene            complement(7507..7905)
                     /locus_tag="VF589_04645"
     CDS             complement(7507..7905)
                     /locus_tag="VF589_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256898.1"
                     /translation="MKKLILIALAGIAGASIAAQPAGDLSPKDESELARALGNRVAGP
                     PTSCVHQRDIRSSRSIGDGLIVFQGRNNSIVYLNEPRARCAGLTRNSALITRTYSTKL
                     CAGDIAEIVEPVSGAGFGSCVLGDFTPYRR"
     gene            complement(7953..8681)
                     /gene="gloB"
                     /locus_tag="VF589_04650"
     CDS             complement(7953..8681)
                     /gene="gloB"
                     /locus_tag="VF589_04650"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX8256899.1"
                     /translation="MVEIVRIAALSDNYVWLMHDPGSGDTAVIDPAVAEPVLAAAAER
                     GWRIGQIWNTHWHPDHTGGNVAVKAATGCSVTGPAAEAARIPGIDRGVSEGDLVRFGA
                     TEAVVLETPAHTAGHISYHLPSEQVIFVGDTLFAMGCGRLFEGDAVQMHANLQRFAKL
                     PPETLVYCAHEYTLSNGRYAAAAEPDNAAVRARLAEVEAARAAGEATVPTTIALELAT
                     NPFMRARSAEELAERRQAKDNFSG"
     gene            complement(8675..9091)
                     /locus_tag="VF589_04655"
     CDS             complement(8675..9091)
                     /locus_tag="VF589_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8256900.1"
                     /translation="MKYLHTMIRVSDPDATLRFFELLGLKELRRFDNEAGRFTLIFLA
                     APGDEDAQVELTHNWGEQGYEGGRNFGHLAYRVDDIYETCQRLMDNGITINRPPRDGH
                     MAFMRTPDGISIELLQAGDRRPPQEPWASMENVGTW"
     gene            9187..9897
                     /locus_tag="VF589_04660"
     CDS             9187..9897
                     /locus_tag="VF589_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672005.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8256901.1"
                     /translation="MTGDRGKAWLIAAAIVALGIVIGGFLLGDGLRRARMADRSVTVR
                     GLAERDVTADLATWTINYTEQGTELSGVQTAIDQDTRAIGDFFRRAGFKDQEVSNAGV
                     SVNQFFDSNRGENSVTVRRKLQLRTNDVTKAQAAYARQFDLIRGGVAIEEGSDIVYSF
                     TKLNDVKPAMIAEATKDARRGAQQFANDSQTEVGGIKQATQGYFSIGARHGNEGDTGN
                     DSPFQKVRVVTTTDFYLD"
     gene            complement(9885..10703)
                     /locus_tag="VF589_04665"
     CDS             complement(9885..10703)
                     /locus_tag="VF589_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256902.1"
                     /translation="MIPLLLILAAAQAAPVPQAAPAPQAAPGPRAVPAPIPQAAPAAD
                     TSAQCLAVVKANPSHAVQVASDWRLKGGGVPAQQCLGLAYAALERWPAAATAFEQAAA
                     EAESRQDPRRGDLWVQAGNSWLAAGDGAKARKAFDAALVTTLLTQEMRGEVHLDRGRA
                     GVALGDLAGARADIDKGLQLVPADPFGWYLSSALALKERRLARAQDDIAEAVRLAPDD
                     ANLLLHAGNVAGISGEVEAARGLYAQAIKAAPNSPAAKAARTALAANAEPAQSR"
     gene            complement(10700..11440)
                     /locus_tag="VF589_04670"
     CDS             complement(10700..11440)
                     /locus_tag="VF589_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256903.1"
                     /translation="MDDLSYLEIGPDLRLAYCHRPGRGPTLVFLPGYMSDMEGGKATA
                     LDAWAESQSRAMLRFDYSGCGASSGDFEAQSLANWRNDALAMIDEIVEGPVVLVGSSM
                     GGWLMLLAALARPERVAGLVGIAAAPDFTAWGFTQEEKLAILQQGRLEQPSPYGEQPY
                     VTTRTFWESGETLRLLHAPVAIDCPVRLLHGQADADVAWAWSLELMKQLRSSDVQATF
                     IKDGNHRLSRGADIALLIATVNGLLELL"
     gene            11557..>11715
                     /locus_tag="VF589_04675"
     CDS             11557..>11715
                     /locus_tag="VF589_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX8256904.1"
                     /translation="MTQLLKISLPDGSVREVAEGTTPADIAAAIGPGLAKAALAARVN
                     GEVRDIARP"
BASE COUNT         1814 a         3959 c         4135 g         1807 t
ORIGIN      
        1 tcctgctcga tgtcggagga gaagccccat tcataggtgg aggcgcgatc ggccgcctcc
       61 tgggcttcgc ggtttctaac gtcggtcact tcacttcacc ctgtgccaat gtcgcgaggc
      121 tgacgccctg cagcgcgccg cggacggcgc cgttcaccac gttgagatgc ggcttgacca
      181 ggcaatggcc gtcgagcgcg cagtcgttcc tcttctcgtc gacgcatacg gtcatcgcga
      241 tcggcccctc gaccgcctcg atgatgtcgg cgaggctgat cccctgcgcc tcgcgggcga
      301 gacggaaacc gccgccggtg ccgcgggccg agctgagcag gccggccgcg gccagccggc
      361 ccatcagttt ctgcgcggtc ggcagcggta cgccggtctc ctcggcgagc agccccgccg
      421 acagccgcgc ttcgccgcca tggcgggcag cggcggccat catcacgacg gcataatcgg
      481 caagggaggt caggcgcatg gaggcaatcg gatcgtttca gtccgattgc catgtgggga
      541 ggagggccgc cgagtcaagc cgtgccccgg cgcaggccgg gacgccgccg tcaccctgaa
      601 cttggttcag ggtctaattc tccgcccgcg gcgcggctcg aaggtggatg ctgaaacaag
      661 ttcagcatga cagggcttgt gatgaacgcc gtccgtgcgc cttggcttac gccttcgtcc
      721 gtgccaccca gtcgcggacg ttgcgttcca tgatgtcgag ggggacggcg cccgagagca
      781 ggaccgcgtc gtggaagccc ttgatgtcga agcggccgcc gagctcgccc cgcgcccact
      841 cgcgcaattc catgatcttg aggcggccga ccatataggc ggtcgcctgg cccggaaaga
      901 tgacgtagcg ctcgatcgcc ttgacgacgc cgcccggcgc gtcggccgaa ttgtcctcga
      961 catattttat cgcccgctcg cgggtccagc gcttgtcgtg gaggccggtg tcgacgacca
     1021 gccggatcgc gcggtggagc tcgagctgga gccggccgaa gtcgcgatag ggatcctggt
     1081 acatgcccat gtctttggcg agccgctcgg catagaggcc ccagccttcg gaccaggcgg
     1141 tgtagccgcc gaagcggcgg aaggcgggca gattctcgag ccgggtggtg acggcgccgt
     1201 cgagatggtg gccgggaatg ccctcatggt aagcgagcgc ctcgacctcg gtgttgggca
     1261 tgtcgcccat ggcgtagagg ttgacgtaat agacgccggg gcgggagccg tccggcgccg
     1321 ggtcctggta gaaggccttg ccggcggatt tctcgcggaa cggctccacc gccttgacgg
     1381 tgaggggggc cttgggcgtg gtggcggggc tgaagaactc cggcacgcgc gcggtgatcg
     1441 cgtcgatcgc cttctgggtc tcgtcgagat attcctggcg gccggcgggc gtgttggcga
     1501 agtagttttg cggacgcttg cgcatgtgga cgaagaagtc ctggagcgag cccttgaagc
     1561 cgatccgctg ctggacctcg cgcatctcgt cgtggatgcg cgcgacctgg cgcaggccga
     1621 gatcgtggac ttcggcgggg ctcatgccgg tggtggtgta atagccgagc cgctcggcat
     1681 aatattcggc gccgcgcgga agccgccaga tgccgtcgtc ggtgccggcg agcttttgct
     1741 ggcgctccat ctcggtgatg acgcgctgat aggcgggctt gacgtcgttg agcagcgcct
     1801 ggcggccggc ggcgatcagg cgcgtcttct caggagcggc gacggtgagc gcgtccacct
     1861 tcttcttgaa gtccgcgaac agcgcggaat cggcgccgga ggtgaagggc gcgcccttga
     1921 tgacgttgcg ggcgtcgttg atgacataag ggtagaccca tttcggcggc agcacgccga
     1981 gcgcggcgcg ctcgcgcgat tcggcgagga cctggtcgat caccgtgtcc acggcggcga
     2041 gccggctgac ataggcttcg gcggtcggca cgtcggtgac gcggtggatg ttgatcagga
     2101 aggcggggag ctggctctgc gcgccgttca tctggtcgaa cgtgtagctg ttcctgcgga
     2161 actcgaaggc gcgctcgccg cgcgttacca tattctcgaa taaatcgtag gacaggcggt
     2221 cggccgggct gagcgcgtcg cggtcgaact gcgtcttcat cgcggccagg ctggtgcggt
     2281 cgagctcctg ctcgcgctgg gcgccggcct cgctgaaatc gccccaggcg ccgtaatcct
     2341 cgtcgacgat gccgcggaac gacttgccga gcggagagag ttcgagcgcg gccttgtcat
     2401 aggcgtcgaa gaaggcggcg agctcggcgc tgtcctgcgc gggcgccgcg acaggggcgg
     2461 cggccggcgc gggcggcgcg gcgctggccg attcgaccgg cggcgcggcg ggcgtggcgg
     2521 cgcaggcggc cagcagggcg gtcgaggcga gcagagcgat ccgaatcatc gggttaggag
     2581 tccttcctgt attagggtta acatacacta atagggaaaa ggcggctttg accagtggcg
     2641 gcgttcgggc catagccgcg ccgccatggc gctctacccg ctcctccgcc cgctcgtctt
     2701 cgcgctcgac gccgagcagg cgcaccgcct gaccctccgc gcgctgaagc tgaagccggc
     2761 ggggcggccg ccggagcgcg acgcccggct cggcatccgc gtcgccggct tgaatttccc
     2821 gaacccgctc ggcctcgccg ccggcttcga caaggatgcc gaggtgttcg agcagatcct
     2881 ggggctcggc ttcggctttg ccgaggtcgg cacgctgacc ccgctcccgc aggcgggcaa
     2941 ccccaagccg cgcctgttcc ggctcgcgga ggaccgggcg gtgatcaacc gcatgggctt
     3001 caacaatgag ggccagcagg cggcgctgca gcggctccag gcgcgcaagc gcgtgtcgcc
     3061 ttcgcggcgg cgtccgacgg aggccaaagc gggcatcgtc ggcgtcaata tcggcgccaa
     3121 caaggacagc gccgatcgca tcgccgatta ttcggcggga gtcgcggcga tgacgggggt
     3181 cgcagattat ctcaccgtca acatctcgtc gcccaacacg ccgggcctgc gcgacctgca
     3241 gagcggatcg gcgctggacg agctgctcgc ggcgatcgcg gaggtaaggg gcgccgggcc
     3301 gccgatcttc gtcaaggtcg cgccggacct cgatccggcg cagatcgacg cgatcgtcgc
     3361 ggccgccgtc gctcggaaga tggacggcat catcgtcgcc aacaccacga tcggccggcc
     3421 cgacctgcgc tcgcggttcc gggacgaggc gggcggtctg tcgggcgcgc ccttgaaggc
     3481 gctggcgctg gagcggctgc gcgatttccg gcgggcgagc ggcggcaggc tgccgctgat
     3541 cgcggccggc gggatcgaga atggcgtcga tgcctatgcc cgcattcgcg ccggcgcgag
     3601 cctggtgcag gtctattcgg ccttggtcta tcggggcccc ggcctcgccc ggcatatatt
     3661 gggcgaactc aggacgctgc tccggcgcga cggtttccgc accctggccg aagcggtggg
     3721 tacggaatag gtctgcggct ccccggcgga ggccgagccc agacccaacc atcctctgga
     3781 cccccgcttt cggggggaac gtattcttct ttcccaaatt ctgctggaag gcctatcgcg
     3841 gcgccatgac gtgcctcaac ccgctccgca ttctgctccc gctcctgctc ctcgccgctt
     3901 gcgcgatcca gccggtcaac ggcgcgcccg tcgagacggc cgctcgcgcc ggcggggtcg
     3961 tttcggccgc cgatccgcgc gcggcggagg ccggccgcta tatcctcaac gccggcggca
     4021 gcgccgccga cgccgcggtc gcgaccatgc tggccttgac cgtggtcgag ccgcaatcgt
     4081 cgggcatcgg cggcggcagc ttcctcgtct atcatgacga ggcggcggac cggatttcga
     4141 cctatgacgg ccgcgaggaa gcgcccgccg ccgcgacgcc gcgcctgttc ctggggccga
     4201 acggcgagcc gctgccgttc ggggaggcga tgcccggcgg caagagcgtc ggcgtgcccg
     4261 gcaatttgcg cgtcgcggcg atgatccacg agcgccacgg caagctgccc tgggcgcggc
     4321 tgttcgaacc cgccatccgc ctcgccagcc agggcttcgc gatcacgccg cgcatgcacg
     4381 gcatgctcgg cggccgcgcc ggccatgccg gcatgacggc ctgggcgcgc gcgcaatatt
     4441 tcggcgcgga cggtcccaag ccggtcggaa ccatcgtgcg caaccccgag ctggccaggc
     4501 tctacgagac catcgccgcc cgcggcgccg actatttcta caccggcccg cccgcgcaaa
     4561 acctggtccg caccgtcacc acggcggcgc gcaacccgtc gtcgatgacg ctcggcgacc
     4621 tcgccagcta cgacgccaag gagcggccgg tgctatgcgg cgaatatcgc cggcaccgca
     4681 tctgcggcat gggcccgccc tcgtcgggag cgaccaccgt gttcgcgatc ctgaagcagc
     4741 tcgagcggtt cgacatggcc gcgatgcggc cgagcgaccc ggcggcgtgg cacctgatcg
     4801 ccgaatcgat gcgcctcgcc tacgccgacc gcgaagccta tatcggcgac ccggatttcg
     4861 tgcgcgtgcc gagcgccggg ctgatggatc ccgcctatct ggccgcgcgc tcgcggctga
     4921 tctcgcccgc ccgcgcgatg cggagcgccg ccgcggggac gccgccgggc gcgcctcggg
     4981 tgacggcggc ggccgacggc gaggtgccgt ccacctcgca cttcgtggcg gtcgacgcgg
     5041 ccggcaacgt cgcctcgatg acctcgacca tcgagggcgt gtggggctcg ggcctcaccg
     5101 tcgacggcta ttatctcaac aacgagctca ccgacttcag cttcgcgccc gaaatcgacg
     5161 gggcgccggt cgcgaacagg gtcgaaggcg gcaagcggcc gcgcagctcg atgtcgccga
     5221 ccatcgtcta cggccccgac ggggaggtcc ggctcgccgt cggagcggcc ggcggaccga
     5281 ccatcatcgc ccaggtcgcc aaggcgatca tcggcgtggt cgactggaag ctttccgccc
     5341 aggaagcgct cgcgcttccg cagatcgtgg cgatcggcga caccgtccgg gtcgagcgcg
     5401 gcaccgcgct ggagccgatg atcccggcgc tgaccgcgct cggccaccgc gccgtcgccg
     5461 ccgatccggg cttcaaggcc aatgccgccg agcgcgtcgg cggacgctgg cgcggcgccg
     5521 ccgatcccag gagcgaaggc gtggcgctgg cgcaatgagc cgcttgccgt gatgccgaac
     5581 ttgtttcagc atccaattcc gagcttcacc gcaggtggag acatggaccc tgaaacaagt
     5641 tcagggtgac ggccgaatgg gggcaagggg cgaggatgca ggcgttcgag cagtttccga
     5701 acctggtgac gatgttcttc gcccgcgccg ccgagaaggg cgaggcgccg tttctctggg
     5761 ccaagaagga aggccagtgg cgctcgacca gctggcgcga ggccgccgag accgtcgccg
     5821 cgctggcggc tgggctgaag cggatcggcc tcggccgcgg cgatacggtg atgctggtgt
     5881 cggagaaccg gccggaatgg tgcatcgccg acctcgccat catggcggcg ggctgcgtca
     5941 ccgtccccgc ctatgtcacc aacacccagc gcgaccacca gcatatcctc gacgacagcg
     6001 gcgccgccgc cgtgatcgtc tcgtcggcca agctcgccaa gccgctgctg ccggcggtgc
     6061 tgcgcgccaa ttcctgcgag catgtgatcg cgatcgaccc gatccatgcc ggccagtcgg
     6121 gcaccgtcca atatcatgac tggcacaagc tgatcgccgc cgaagcgccc gacgtcgccg
     6181 ccaccgcggc cgcggcgacc ttcggccgcg gcgacctagc ctgcatcatc tacacgtccg
     6241 gcaccggcgg cgcgccgcgc ggcgtgatgc agcatcacgg cgcgatcctc cacaatgtcg
     6301 aaggctgcat cgacgtcatc gccgaggatt tcgggctgga cgaggaggtg ttcctctcct
     6361 tccttcccgc cagccacgct tacgagcata gcggcgggca attcttcccg atcgggatcg
     6421 gcggccagat ctattattcg gaggggctcg agaagctcgc ctccaatatc gaggaggtgc
     6481 ggccgaccat catggtggtg gtgccgcggc tgttcgaagt gctgcgccag cgcatcatga
     6541 agaacgtcga gaagcagggg aagatcacca attacctgct ccgccgggcg ctcgacatcg
     6601 gcgccaagga ttatgcggga caggtgccgc tgtgggaccg gccgatggac ttcatcctcg
     6661 gccgcacgct tcgtccgaag gtcagcgcgc ggttcggcgg gcggatcaag gcgatggtgt
     6721 cgggcggggc gccgctcaac cccgaggtcg gcatcttctt ccattcgatg ggcctcaccc
     6781 tgctgcaggg ctacggccag accgaggcgg cgccggtgat cagctgcaac cggcccaggg
     6841 cgggcatcaa gatggacacg gtcggcccgc cgcttaagaa taccgaggtg cggatcgccg
     6901 aggacggcga gatattggtg cgcggcgagc tcgtcatgaa gggctattgg cgcaacccgg
     6961 cgctgaccga gcgtacgatc gtcgacggct ggctccacac cggcgacgtc ggccatatcg
     7021 acgcgaaggg ccggatcgtc atcaccgacc gcaaaaagga cctcatcatc aacgacaagg
     7081 gcgacaatgt cgcgccgcag aagatcgagg gcatgctcac cctccagccc gagatcgtgc
     7141 aggcgatggt ggcgggcgac aagaagccct atgtggtcgg cctggtcgtc cccgatcccg
     7201 aatggaccgg cgaatgggcc aaggcggccg gcaagcccgc cgatgccgcg gcgttgcgcg
     7261 atgacagtga ttatctgaag gcggtctcag ccgcggtcga ccgggtcaat gccgagctgt
     7321 cggtgatcga gaaggtgcgc cgcttcatcc tcgccgacga gccgttcacg accgacaacg
     7381 agcaattgac gccgtcgatt aagattcgcc gccacgtgct gcgccaggtc tatggcgagc
     7441 ggctggacgc gctctaccgc cgctagcgcc ggagggcgct ctaccgccgg tagcgccgag
     7501 gtcgcgctac cgccggtagg gcgtgaagtc gccgagcacg cagctgccga agccggcgcc
     7561 gctgaccggc tcgacgatct cggcaatgtc gcccgcgcac aatttggtgc tgtaggtgcg
     7621 ggtgatgagg gcgctgttgc gggtgaggcc ggcgcagcgg gcgcgcggct cgttcaaata
     7681 gacgatgctg ttgttgcggc cctggaagac gatcagtccg tcgccgatgc ttcggctcga
     7741 gcgtatgtcg cgctggtgga cgcagctcgt cggcggcccg gccacgcggt tgccgagcgc
     7801 gcgcgccagt tcgctctcgt ccttgggact gaggtctccg gcaggttgcg cggcaatcga
     7861 agcgccggcg attccggcca acgcgatcag gatcagcttc ttcatcatcg cctccattgg
     7921 agcgacaacg ctccgaccta cgactcggcc cctcagccgc tgaaattatc cttggcttgc
     7981 cgccgctcgg caagctcctc ggccgagcgc gcccgcatga acgggttggt ggcgagctcg
     8041 agcgcgatcg tcgtcggcac cgtcgcctcg cccgccgccc gcgccgcctc gacttcggcc
     8101 agccgggcgc ggacggcggc attgtccggc tccgccgccg ccgcatagcg gccgttggag
     8161 agcgtatatt cgtgggcgca atagaccaaa gtctcaggcg gcagcttggc gaagcgctgg
     8221 aggttggcgt gcatctggac ggcatcgcct tcgaacagcc ggccgcagcc catcgcgaat
     8281 aaggtgtcgc cgacgaagat cacctgctcg gaaggcagat gataggagat atggccggcg
     8341 gtatgggccg gggtttcgag caccaccgcc tcggtggcgc cgaaccggac caggtcgcct
     8401 tcgctcacgc cgcggtcgat gccggggatg cgcgcggcct cggcggccgg gccggtgacg
     8461 gagcagccgg tggcggcctt gaccgctaca ttgccgccgg tgtgatcggg gtgccaatgc
     8521 gtgttccaga tctggccgat ccgccagccg cgctccgccg ccgccgccag cacgggctcg
     8581 gcgacggccg gatcgatcac cgccgtgtcg cccgagccgg gatcgtgcat cagccacaca
     8641 taattgtcgc tgagcgccgc gatgcggacg atctctacca cgtgccgaca ttctccatgc
     8701 ttgcccaggg ctcctgcggc ggccgccgat cgccggcctg gagcagctcg atcgaaatgc
     8761 cgtcgggcgt gcgcatgaac gccatatggc cgtcgcgcgg cggccggttg atggtgatcc
     8821 cattgtccat cagccgctgg caggtctcgt aaatgtcgtc gacgcggtag gcgagatggc
     8881 cgaaattgcg gccgccttca tagccctgct caccccaatt gtgggtgagc tcgacctgcg
     8941 cgtcctcgtc gccgggcgcg gcgaggaaga tgagggtgaa gcggcccgcc tcattgtcga
     9001 agcggcggag ttccttgagg cccagcagct cgaagaagcg gagggtggcg tcgggatcgg
     9061 agacgcggat catcgtatgc aaatacttca ttacggctcc tcaattgttt ccgctatcgg
     9121 ggtggcccta ttgccgctat tatgcgcgcg gggcggcgaa cgttccacct tcgaaggggg
     9181 acagggatga cgggcgatcg cggcaaagcg tggctgatcg cggcggcgat cgtggctctc
     9241 ggcatcgtca tcggcggctt cctgctcggc gatggcctgc gccgcgcgcg catggccgac
     9301 cgttcggtga ccgtgcgcgg gcttgccgaa cgcgacgtca ccgccgacct cgccacctgg
     9361 acgatcaact ataccgagca gggcaccgag ctcagcggcg tgcagactgc gatcgaccag
     9421 gatacgcgcg cgatcggcga cttcttccgg cgcgcgggct tcaaggacca ggaggtcagc
     9481 aacgccggcg tcagcgtcaa ccagttcttc gacagcaacc gcggcgagaa cagcgtcacg
     9541 gtgcggcgca agctccagct tcggaccaac gacgtcacga aggcgcaggc cgcctatgcg
     9601 cgccagttcg acctcatccg cggcggcgtc gcgatcgagg aaggctccga catcgtctac
     9661 agcttcacga agctcaacga cgtcaaaccg gccatgatcg ccgaagcgac caaggatgcg
     9721 cggcgcggcg cccagcaatt cgccaacgat tcgcagaccg aggtcggcgg catcaagcag
     9781 gcgacgcagg gctatttctc gatcggggcc cgccacggca acgagggcga caccggcaac
     9841 gattcaccct tccagaaggt gcgggtcgtc accacgaccg acttctacct cgactgagcc
     9901 ggctccgcat tggcggcgag cgcggtcctc gccgccttgg cggccggcga attgggcgcg
     9961 gctttgatcg cctgcgcgta cagcccgcgc gccgcctcga cctcgccgga aatgccggcg
    10021 acgttgccgg cgtggaggag cagattggca tcgtcgggcg ccagccgcac cgcctcggcg
    10081 atatcgtcct gcgcgcgggc cagccgcctt tccttcagcg ccagcgccga ggagagatac
    10141 cagccgaacg ggtcggcggg gacgagctgg aggcccttgt cgatatcggc ccgcgccccg
    10201 gcgaggtcgc cgagggcgac gccggcgcgg ccgcgatcga gatggacctc gccgcgcatc
    10261 tcctgggtga gcagggtggt gacgagagca gcgtcgaacg ccttgcgcgc cttggcgccg
    10321 tcgcccgcgg cgagccagct attgccggcc tgcacccaca agtcgccgcg gcgcggatcc
    10381 tgccggctct ccgcttcggc cgcggcctgc tcgaaggcgg tggcggccgc gggccagcgc
    10441 tccagcgcgg cataagcgag gccgaggcat tgctgggccg gaaccccgcc acccttcagc
    10501 cgccagtcgc tggccacctg gacggcgtgg ctcggattgg ccttcacgac cgcgaggcac
    10561 tgggccgacg tatcggcggc gggcgcggcc tgggggatgg gggcgggcac cgctcggggc
    10621 ccaggcgcgg cttgaggggc gggcgcggcc tgggggacgg gcgcggcctg ggcggcggcc
    10681 aggatcagca gcaatgggat cacagaagct ccaatagtcc gttcacggtc gcgatcagga
    10741 gggcgatgtc cgccccgcgc gacaggcggt ggttgccgtc cttgatgaat gtcgcctgca
    10801 catcggatga acgcagctgc ttcatcagct cgagcgacca ggcccaggcc acgtcggcat
    10861 cggcctggcc gtggagcagc cgcaccgggc aatcgatcgc gaccggagca tggagcagcc
    10921 gcagcgtctc accgctctcc cagaaggtgc gcgtggtgac atagggctgc tcgccataag
    10981 gcgagggctg ttcgagcctg ccctgctgca ggatcgccag cttctcctcc tgcgtgaagc
    11041 cccaggcggt gaaatcgggc gccgcggcga tgccgaccag gccagccacc cgctccggcc
    11101 gggccagcgc cgccagcagc atcagccatc cgcccatcga ggagccgacc agcaccaccg
    11161 gcccctcgac gatctcgtcg atcatggcga gcgcgtcgtt gcgccaattg gccaggctct
    11221 gcgcctcgaa gtcgccgctg ctggcgccgc agcccgaata atcgaagcgc agcatcgccc
    11281 ggctttggct ttccgcccag gcgtcgagcg cggtcgcctt gccgccctcc atgtccgaca
    11341 tatagccggg caggaacacg agggtggggc cgcggccggg gcggtggcaa taagcgagcc
    11401 gaaggtcggg gccgatttca aggtaggaga gatcatccat cgcgcgccgc tagcacagcc
    11461 gccggggatg ggaaaaccgg cgggacatga ccgccgccgc gccgcaacgc cgcaagggct
    11521 ggaggaagcc ggtgcgcatc gctacatggc gccactatga cgcagctttt gaagatcagc
    11581 cttcccgacg gttcggtgcg cgaggtcgcg gaagggacca cgccggccga catcgccgcc
    11641 gcgatcggac ccggcctcgc caaggcggca ctggcggcgc gggtcaatgg cgaggtgcgc
    11701 gacatcgcgc gcccg
//
LOCUS       DASVRK010000122         7889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_208503, whole genome shotgun sequence.
ACCESSION   DASVRK010000122 DASVRK010000000
VERSION     DASVRK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208503"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..721
                     /locus_tag="VF589_04680"
     CDS             206..721
                     /locus_tag="VF589_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX8256905.1"
                     /translation="MAGLTALDVLVILLVGGGLVLGFLRGFVTEILSLLAWVAAILAL
                     KALHGPAAAMLAPTVGAGAAVLAFALVFGLVFLAGKLVARRIGAATRRSVVGPVDRVL
                     GGGFGALKGLIGATLIYLGASLVYDAIYTSRAERPEWMAQSRTYPLLHASGRAIVDFV
                     DARRRAPAAAE"
     gene            718..2031
                     /gene="cysS"
                     /locus_tag="VF589_04685"
     CDS             718..2031
                     /gene="cysS"
                     /locus_tag="VF589_04685"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX8256906.1"
                     /translation="MTQLRLYDTMARAKRDFEPVDPNRVTMYVCGPTVYNRAHIGNAR
                     PAVVFDVLARLLRFLYPEREIVHAANITDIEDKIMAAAAAEGVPIEVITERYGRFYSE
                     DLGALGVARPDIVPLATQHIPQMVAMIERLIAAGHAYAAEGHVLFDVPSWPDYGRLSG
                     RPMDEMIAGARVDVAPYKRSPADFVLWKPSADGQPGWDSPWGRGRPGWHIECSAMIEA
                     HLGQTIDIHGGGIDLEFPHHENEIAQSSCAHGGAPLARYWLHNGMLSMASSKMSKSLG
                     NVVTVGELLGEGHKGEALRFALLSAHYRQPLDWSPQLIAQSKATLDRLYRTAGDADPG
                     TPSPAVVDALADDLNTPLALSRLSAIEDGATLRASAALLGLLGDSAAAWFQGAGDSRI
                     DALVAARAEAKQRRDFAEADRIRAQLAGEGIMLEDNPTGTTWRRA"
     gene            2619..3044
                     /locus_tag="VF589_04690"
     CDS             2619..3044
                     /locus_tag="VF589_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX8256907.1"
                     /translation="MTTALYNRDILRLAAAIPHQRRLDRPQASAEKSSPVCGSRVAVD
                     VVLDGEGRVTALGQEVKACALGQASAALMGAHAVGRDKAELARARDALAAYLAGERAD
                     PGAWPGLDILEPARPYPARHASILLAFEAVAEAADRASH"
     gene            3044..4885
                     /locus_tag="VF589_04695"
     CDS             3044..4885
                     /locus_tag="VF589_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX8256908.1"
                     /translation="MEAAGAEAAAALVLRDGVVMLGAAMLFVTLFRRLGLGAVLGYLI
                     AGALVGPGGLGLVDARDEMLRIADFGIVLLLFLVGLELHPSRLWRLRHDIFGLGLLQV
                     ALSGLALAALVYAVTGFSWAAALAIGLPLALSSTAQVLPALRSTGDINTPSGERAFSI
                     LLFQDLSIVPLITIIAALSRAPPDPVAPPGWQLGLYTVGAIVGLVLAGRFLINPLFRV
                     VGRVSERELFVVAGLFTVLAAAAVMETLHLSTALGAFIAGVMLADSPYRHELEVDIEP
                     FRSILLGLFFVAVGMMLDLGAIAQRPLFVLGMALALISVKAAILFGVAKLFRMETRRA
                     VKLGLLLSQGGEFAFVLFAAAQAALLIEPAAASLFSAIVTISMASTPFLMMFNDWFDR
                     RSRLRDGDDMEGPEQSPESRAIVIGYGRFGQTVSQMLMAKGIALTLIDSKPSQIELAG
                     SFDMKVYYGDGMRLDLLRAAGAGEAQALLFCLDDGKPSPAKLEPILEAFPQAAVFVRV
                     FDRRHWIDIAGHDVAGIYREVFESAVLMGRDALRAFDQSDEEVERVELAYRDRDAERL
                     EGQSKSGDLHTLMDRMFRPDSMLADDGEAGQGDSESRKASTSAGSTE"
     gene            complement(4831..5928)
                     /gene="aroB"
                     /locus_tag="VF589_04700"
     CDS             complement(4831..5928)
                     /gene="aroB"
                     /locus_tag="VF589_04700"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX8256909.1"
                     /translation="MSEVRVGLGQRSYTISIGHGMLSRAAALLEPFARGRRLIVVTDA
                     VVWGAAGAAVEAALAEFELVPILVPAGEAGKSWASLIELCERLLALEVERSDAIVALG
                     GGAVGDLAGFAAAIVKRGCHYVQIPTTLLAQVDSSVGGKTAINAAAGKNLVGAFHQPA
                     AVLIDTALLATLPDRQLRAGYAEIVKYGLIGDPAFFAWCEDHGEKLIGGDVAARLQAI
                     ETSVRAKAAIVAEDERETMGRRALLNLGHTFGHAIEAEAGFSGRLLHGEAVSIGMALA
                     FRFSAKRRLCPPEDAARVAAHLKRAGLPVTLAEARIAASGRRLLGHMANDKKRARGRL
                     PFILARGIGAAFVDHSVEPAEVEAFLDSESP"
     gene            complement(5925..6467)
                     /locus_tag="VF589_04705"
     CDS             complement(5925..6467)
                     /locus_tag="VF589_04705"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX8256910.1"
                     /translation="MSEALPRIDRSIVLVGLMGAGKSTVGKRLARRLGLPFVDSDEEI
                     ERAADRSVAEIFSDYGEASFRDGERRVIARLIEGAPKVIATGGGAFVDPETRALILGR
                     CIAVWLDADLETLAERVSRRDHRPLIKDCEPLPILKGLADARNPIYAEAHLHVQSQPA
                     PHERAVERIVAALGAWEQRP"
     gene            6516..6689
                     /locus_tag="VF589_04710"
     CDS             6516..6689
                     /locus_tag="VF589_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256911.1"
                     /translation="MRRSSFAPARRRRSPLPAILIILLVLLIGGAIYLTTVDTEVPQQ
                     RIEQDVTNEALAK"
     gene            6670..>7889
                     /locus_tag="VF589_04715"
     CDS             6670..>7889
                     /locus_tag="VF589_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256912.1"
                     /translation="MKPWRSKLFLLGGAAALALAIPALGQEAPESLLPPGFGDPAGPP
                     PPPQQQAPPPGTPAPSATPGAPAGPVPPERRAPPPRQPVISSGEEDLAALEALLPDPI
                     EIPEASRRPVEIIGPLGPENGGLGMGAFGNANGRLLSTLMRRLDAPLPSRWTSILLRR
                     ALLTRAPAPAYVNPVDWTAERAWLLLRMGEADAARMLVQAVDVDRFTPKMFTVAVQSS
                     LANADPLGLCPLIGPGRKTSDEPVWALGDAMCAALEGEPAKASTLIGQARRRSLAPAI
                     DLTLAEKVIGAGENTRRAVTVQWDDVGELNSWRFGLASATGIQIPEPLMNGAGSHVHA
                     WLARAPMIPLEQRVMAAQVAASLGVFSSASLVEMHSLLADETDPSELAETVGGRLRLA
                     YAGRTLDDRLGALRG"
BASE COUNT         1112 a         2948 c         2600 g         1229 t
ORIGIN      
        1 cgccgccgca ggcggtggtg gagggggccc cggccgagac acgcgggttt ttgtcggcct
       61 gaacctgttt cagagcctgc cccggacccg atccgggggt ccatccctcc acccgagcga
      121 cgctccacca tggatgctga aacaagttca gcatgacggc accatgacgc ttccctctaa
      181 aatcatcctg ctctagcaac cctccatggc cggcctcacc gctctcgacg tcctcgtcat
      241 cctgctcgtc ggcggcgggc tggtgctcgg cttcctgcgc ggcttcgtga cggagatatt
      301 gtcgctgctc gcctgggtcg ccgccatcct cgcgctgaag gcgctgcacg ggccagccgc
      361 cgcaatgctc gcccccacgg tcggcgccgg cgcggcggtg cttgcctttg cgctcgtctt
      421 cggcctcgtc ttcctggccg gcaagctggt cgcccgccgc atcggcgccg ccacccgccg
      481 ctcggtggtc ggcccggtcg accgcgtgct cggcggcggc ttcggcgcgc tcaaggggtt
      541 gatcggcgcc accctcatct atctcggcgc cagcctcgtc tacgacgcca tctacaccag
      601 ccgcgccgag cggcccgaat ggatggcgca gtcgcgcacc tatcccctgc ttcacgccag
      661 cggccgcgcc attgtcgatt tcgtcgacgc ccgccgccgc gccccggccg ccgccgaatg
      721 acccagctcc gcctctacga cacgatggcg cgcgccaagc gcgacttcga gccggtcgac
      781 ccgaaccgcg tcacgatgta tgtctgcggc ccgaccgtct acaaccgcgc ccatatcggc
      841 aacgcccgcc ccgccgtggt gttcgacgtt ctcgcccgcc tgctccgctt cctctatccg
      901 gagcgcgaga tcgtccacgc cgccaacatc accgatatcg aagacaagat catggccgcc
      961 gccgctgccg agggcgtgcc gatcgaggtc atcaccgagc gctacggccg cttctattcc
     1021 gaggatctcg gcgccttggg cgtcgcccgg cccgacatcg tcccgctcgc cacccagcac
     1081 atcccgcaga tggtggcgat gatcgagcgc ctgatcgccg ccggccatgc ctatgcggcc
     1141 gagggccatg tgctgttcga cgtgccgagc tggcccgatt acggcaggct ctccgggcgg
     1201 ccgatggacg agatgatcgc cggcgcccgc gtcgacgtcg ccccctacaa gcgctcaccg
     1261 gccgatttcg tgctgtggaa gccgtccgcc gacggccagc ccggctggga cagcccgtgg
     1321 ggccgcggcc gcccaggctg gcacatcgaa tgctcggcga tgatcgaagc ccatctcggc
     1381 cagaccatcg acatccatgg cggcggcatc gacctcgaat tcccgcacca tgagaatgag
     1441 atcgcccagt cgagctgcgc ccatggcggc gccccgctcg cccgctactg gctccacaac
     1501 ggcatgctct cgatggcatc gagcaagatg tcgaagagcc tcggcaacgt cgtcaccgtc
     1561 ggcgaactgc tcggcgaagg ccacaagggc gaggctttgc gtttcgcctt gctctccgcc
     1621 cattaccgcc agccgctcga ttggtcgccg cagctgatcg cccagagcaa agccacgctc
     1681 gaccggcttt accgcaccgc cggcgatgcc gatcccggca cgccgagccc ggccgtggtc
     1741 gacgccctgg ccgacgatct caacacgccg ctcgcgctct cgcgcctgtc ggcgatcgag
     1801 gacggcgcga cgcttcgcgc cagcgccgcc ttgctcggtc tgctcggcga cagcgccgcg
     1861 gcctggttcc agggcgccgg cgacagccgc atcgacgcct tggtcgccgc ccgcgccgag
     1921 gccaagcagc gccgcgactt cgccgaagcc gatcgcatcc gcgcccaatt ggcgggcgag
     1981 ggcatcatgc tggaggacaa tcccaccggc accacctggc gccgcgcctg accggttccg
     2041 ctggttgcag taatagaacg catcaagaac agacttgaca ttctgcacca aataggctag
     2101 ccagcttttt gtccgcagaa gccgaaggcc aaggtggagt gggcagggat ttccagttcc
     2161 ggttcgtccg ggctggaagc gaccggcgcg gaaccatccg ggaaatgttg gatccgcatc
     2221 aaccggcgcc cggaataacg tttgcacgtg agggcgtcgc cgagcgagcg gcccgagccg
     2281 ttagcatgtg gggagatagg gacttcggga gccctcgaag catgcgaccc aagaacgcgg
     2341 cttggaaagc agtccaaata ggcggctaac tcttaaatcg ccacccgagc cccaagcagg
     2401 gaacagcgtg ccgaccgggc gtctcgaaag cctcgacgct ttcttgaata ttgaactgca
     2461 aaagtcgtcg agccccgggt aaccgtggca ggacgcgccg gtcgaaaccc cctctttttc
     2521 cagggcgcgc acagccgcgc ccgcatcgcc tgtgcgcgcg cgctcatcgc gcccgacacg
     2581 gctgtcaatc gcggaatggc gggctatagg gagccgcaat gaccaccgcg ctctacaatc
     2641 gcgacatcct gcgcctcgcg gccgccatcc cccatcagcg ccggctcgac cggccgcagg
     2701 cgagcgccga gaagagctcg ccggtctgcg gcagccgcgt tgccgtcgac gtcgtgctgg
     2761 acggggaggg cagggtgacc gccttgggcc aggaggtgaa ggcgtgcgcg ctcggccagg
     2821 cctcggccgc cttgatgggc gcccatgccg tcggccgtga caaggccgag ctcgcccgcg
     2881 cccgcgacgc cctggccgcc tatctcgccg gcgagcgcgc cgatcccggc gcgtggcccg
     2941 gcctcgacat cctcgaaccg gcgcggccct atccggcccg gcacgcctcg atcctgctcg
     3001 cctttgaagc ggtcgccgaa gccgccgacc gggcgtcgca ctgatggaag ccgccggcgc
     3061 cgaagccgcc gccgccctgg tgctgcgcga cggcgtcgtc atgctcggcg cggcgatgct
     3121 gttcgtcacc ctgttccgcc ggctcggcct cggcgcggtg ctcggctatc tcatcgccgg
     3181 cgcattggtc ggccccggcg gcctcggcct cgtcgacgcc cgcgacgaga tgctccggat
     3241 cgccgatttc ggcatcgttc tcctcctctt cctggtcggc ctcgagcttc acccgagccg
     3301 cttgtggcgc ctcaggcacg acattttcgg tctcggcctg ctgcaggtcg cgctctccgg
     3361 gctcgcgctg gcggccctgg tctatgcggt gaccggcttc agctgggcgg cggcgctcgc
     3421 catcggcctg ccgctcgctc tgtcctcgac cgcgcaggtg ctgcccgcgc tgcgctcgac
     3481 cggcgacatc aacacgccgt cgggcgagcg cgctttctcg atcctgctct tccaggatct
     3541 gtcgatcgtg ccgctcatca ccatcatcgc ggccctgtcg cgggcgccgc ccgatccggt
     3601 cgcgccgccg ggctggcagc tcgggctcta cacggtcggc gccatcgtcg gcctggtcct
     3661 cgccggccgc ttcctgatca atcccctgtt ccgggtggtc gggcgggtca gcgagcgcga
     3721 attgttcgtc gtcgccggcc tgttcaccgt gctcgccgcc gccgccgtga tggagacgct
     3781 tcatctgtcg accgcgctcg gcgccttcat cgccggcgtg atgctggccg attcgcccta
     3841 ccggcacgag ctcgaggtcg acatcgagcc gttccgctcg atcctgctcg gcctgttctt
     3901 cgtcgcggtc ggcatgatgc tcgatctcgg cgcgatcgcg cagcggccct tgttcgtgct
     3961 cggcatggcg ctcgccctga tcagcgtgaa ggcggcgatc ctgttcgggg tcgccaagct
     4021 gttccggatg gagacccgcc gcgcggtcaa gctcggcctg ctattgagcc agggcggcga
     4081 gttcgcgttc gtgctgttcg cggccgccca ggcggccctg ctgatcgagc ccgccgccgc
     4141 cagcctgttc agcgccatcg tcaccatctc gatggcctcg acgccgttcc tgatgatgtt
     4201 caacgactgg ttcgaccgcc gctcgcggct gcgcgacggc gacgacatgg aggggccgga
     4261 gcagtcgccc gaatcgcgcg cgatcgtgat cggctacggc cgcttcggcc agaccgtctc
     4321 gcagatgctg atggccaagg gcatcgcgct gacgttgatc gattccaagc cgagccagat
     4381 cgagctcgcc ggcagcttcg atatgaaggt ctattatggc gacggcatgc ggctcgacct
     4441 gctccgcgcc gccggagccg gggaggcgca ggccctgctg ttctgcctcg acgacggcaa
     4501 gccgtcgccg gccaagctcg agccgatcct cgaagccttt ccccaggccg ccgtgttcgt
     4561 gcgcgtgttc gaccgccgcc actggatcga cattgccggg cacgacgtcg ccggcatcta
     4621 ccgcgaagtg ttcgaatcgg ccgtcctgat gggccgcgac gcgctccgcg ccttcgatca
     4681 gtccgacgag gaggtcgagc gcgtcgagct cgcctatcgg gaccgcgacg ccgagcggct
     4741 cgaaggccaa agcaagtcgg gcgatctcca caccctgatg gaccgcatgt tccgccccga
     4801 cagcatgctg gccgacgacg gcgaggcggg tcagggcgac tccgaatcga gaaaggcctc
     4861 gacctccgcc ggctcgaccg agtgatcgac gaacgccgcg ccgatgccgc gcgcgaggat
     4921 gaacggcagc cgcccccgcg cccgcttttt gtcgttggcc atatggccga gcaggcgccg
     4981 gcctgaggcc gcgatcctgg cctcggccag cgtgaccgga agccccgccc gcttgaggtg
     5041 ggcggcgacc cgcgccgcat cctccggcgg gcacagtcgc cgcttcgccg agaagcggaa
     5101 ggcgagcgcc atgccgatgg acaccgcctc gccgtgcagc agccgcccgg aaaagcccgc
     5161 ctccgcctcg atcgcatggc cgaacgtatg gccgagattg agcagcgccc gccggcccat
     5221 cgtctcgcgc tcgtcctcgg cgacgatcgc cgccttggcc ctgacgctgg tttcgatcgc
     5281 ctggagccgc gccgcgacgt cgccgccgat cagcttttcc ccatgatcct cgcaccaggc
     5341 gaagaaagcg gggtcgccga tcagcccgta tttgacgatc tcggcatagc cggcgcgcag
     5401 ctgccggtcg ggcagcgtcg ccagcaaggc ggtgtcgatc agcaccgccg cgggctgatg
     5461 aaaggccccg accagattct tgcccgccgc tgcattgatc gcggtcttgc cgccgaccga
     5521 cgaatcgacc tgggcgagca aggtggtcgg tatctgcaca tagtggcagc cgcgcttgac
     5581 gatggcggcg gcgaagccgg ccaggtcgcc gaccgcgcct ccgcccaaag ccacgatcgc
     5641 gtccgagcgt tccacctcca gcgccagcag ccgctcgcac agctcgatca gcgaagccca
     5701 gctcttgccc gcctcgcccg ccggcacgag gatcgggacc agctcgaact ccgccagcgc
     5761 cgcctcgacc gcggctccgg cggcgcccca gaccaccgcg tcggtgacga cgatcagccg
     5821 ccgcccgcgc gcgaacggct ccagcaaagc agccgcccgg ctgagcatgc cgtggccgat
     5881 gctgatcgtg tagctgcgct gacccagccc gacccggacc tcgctcaagg ccgttgttcc
     5941 caagcgccga gcgccgcgac gatccgctcc accgcccgct catgcggcgc cggctggctt
     6001 tggacgtgga gatgggcctc ggcgtagatc gggttgcggg cgtcggcgag gcccttgagg
     6061 atcggcagcg gctcgcaatc cttgatgagc gggcggtggt cgcggcgcga cacgcgctcg
     6121 gccagcgtct cgaggtcggc gtcgagccac acggcgatgc agcggccgag gatcagcgcc
     6181 cgcgtctcgg gatcgacgaa ggcgccgccg ccggtcgcga tcaccttggg cgcgccttcg
     6241 atcagccgcg cgatcacccg cctttcgccg tcgcgaaagc tggcctcgcc ataatcggag
     6301 aagatctcgg cgacgctgcg gtcggcggcg cgttcgatct cctcgtccga atcgacgaag
     6361 ggcaggccga gccgccgggc gaggcgtttg ccgaccgtcg atttgccggc gcccatcagt
     6421 ccgacgagga cgatcgaacg gtcgatgcgg gggagcgctt cggacatgcc gccggggcta
     6481 tacagcgggc ggcatcgtgg ggcaaaaggc gcgccatgcg tagatcctct tttgctcccg
     6541 cccgccggcg gcgctcgccg cttcccgcga tcctcatcat tctgctcgtc ctgctgatcg
     6601 gcggtgcgat ctatctgacg accgtcgaca ccgaggtgcc gcagcagcgg atcgagcagg
     6661 atgtgacgaa tgaagccctg gcgaagtaag cttttcctgc tcggcggcgc ggccgcgctt
     6721 gccctcgcca tcccggcttt gggccaggaa gcgcccgaat cgctgctgcc gcccggcttc
     6781 ggcgaccccg ccggaccgcc gccgccgccg cagcagcagg cgccgccgcc cgggacgccc
     6841 gcgccttcgg ccacccccgg cgcgcccgcg ggaccggtgc cacccgagcg ccgcgcgccg
     6901 ccgccgcgcc agccggtcat ctcgagcggc gaggaggatc tcgccgccct cgaagccttg
     6961 ctgccggacc cgatcgaaat ccccgaggct tcgcgccggc cggtcgagat catcggcccg
     7021 ctcgggcccg aaaatggcgg cctcggcatg ggcgcgttcg gcaacgccaa cgggcggctg
     7081 ctgtcgacgc tgatgcggcg gctcgacgcg ccgctgccgt cgcgctggac ctcgatcctg
     7141 ctgcgccggg cgctgctgac ccgcgcgccc gccccggcct atgtcaatcc ggtcgactgg
     7201 accgccgagc gcgcctggct gctgctgcgc atgggcgagg ccgacgcggc ccggatgctg
     7261 gtccaggcgg tcgacgtcga tcgcttcacg cccaagatgt tcacggtggc ggtgcagtcc
     7321 tcgctcgcca acgccgatcc gctcggcctc tgcccgctga tcggtccggg ccggaagacg
     7381 tccgacgagc cggtctgggc cttgggcgat gcgatgtgcg ccgcgctcga aggcgagccc
     7441 gccaaggcca gcaccctgat cggccaggcg cggcggcgaa gcctcgcccc ggcgatcgac
     7501 ctgacgctgg ccgagaaggt gatcggcgcc ggcgagaata cccgccgtgc ggtcaccgtc
     7561 cagtgggacg acgtcggcga gctcaacagc tggcgcttcg gcctcgccag cgcgaccggc
     7621 atccagatcc cggagccgct gatgaacggc gccggctcgc acgtccatgc ctggctcgcg
     7681 cgggcgccga tgatcccgct cgagcagcgc gtcatggcgg cgcaggtggc ggcctcgctc
     7741 ggcgtcttct cgagcgcctc gctcgtcgag atgcacagcc tgctcgccga cgagaccgat
     7801 ccttcagagc tcgccgagac ggtcggcggc cgcttgcgac tggcctatgc cggccgcacc
     7861 ctcgacgacc ggctcggcgc gctgcgcgg
//
LOCUS       DASVRK010000123         7905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_519680, whole genome shotgun sequence.
ACCESSION   DASVRK010000123 DASVRK010000000
VERSION     DASVRK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519680"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..3585
                     /locus_tag="VF589_04720"
     CDS             154..3585
                     /locus_tag="VF589_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HEX8256913.1"
                     /translation="MSAPDLSLLGKRRFAPLFAVQFLGAFNDNLLKFALLFLANFGLY
                     AAEPDKAEMLATVATGLFILPYFLFSALAGQLADAWDKAKLVRAVKLAEIGIMGLALA
                     GFWSESVPLLLASLFLMGLHSTLFGPVKYSILPQHLESREIMGGTGLIEAGTFLAILG
                     GQLLGGVIPPWEAGLVATGLAIVGFLASLAVPPAPATAPGLRIDFNIFRGTWHILKAA
                     RHGRGVWLSILGISWFFAVGAVLLAEFAPLVSGVLGARQEVATLFLLVFSVSVALGSV
                     MVNRLLAGEVSARYVPVAALALSGFMIDLWLATRGYEIQTAGATIAQFLASGGSWRIL
                     IDLAGIAFAGGMFIVPLYAILQTHSPPAERSRTIAANNIVNAAVTVVMVAVLAALLGS
                     GVDVPAIIGILGGATLAVALVSCWLLPETVIKSLVRGLLRLLYRVEVEGEENMPLPGE
                     RAVVVVNHVSFLDGLLLAAFLPGKPTFAVHTRIAKAWWVRPLLGLFDAFPVDPVNPMA
                     AKAMVRAVKEGRTLVIFPEGRITVTGALMKVFDGPGMVADKADAPIVPVRIDGAQYSP
                     FSRLRGKVRLRSFPKIRLTILPPRRFAIDGTMSARQRRAIAGRRLYDEMSEMIFATSN
                     TGRTLFEALLDARRIHGGKAPIVEDIKREPLSYNRLVAGSMALGRPLARLAAPDEAVG
                     VLLPNVGGVAVTFFALQAAGRVPAMLNYTAGLTNLKAACAAAEIRTIVTARAFVEQAK
                     LADTIAGLEASGLIVAYLEDIGAGIGKGGKLRALAAARFARQIHRAKRVSPDAPAVIL
                     FTSGSEGLPKGVVLTHRNLLANCQQLAARIDFNASDIVLNALPVFHSFGLTGGTLLPI
                     LNGVRTLLYPSPLHYRIVPALAYDANATILFGTDTFLAGYARMAHGYDFYSLRYIFAG
                     AERVRDETRRTYSDKFGLRILEGYGATEAGPVIAVNTPMHFRAGSVGRLLPGITARLD
                     PVPGIAEGGRLAIRGPNIMAGYLKADAPGLLQPPEGGWHDSGDIVTIDEEGFVTIRGR
                     AKRFAKIGGEMVSLPAVEGYAAAVWPEADHAVVTRPDPRKGEQLVLFTTRADADAGAL
                     QAWARANGVTELMIPRDIRVLDALPVLGTGKLDYVAMGEAAALSPQGGKLQPAAV"
     gene            3657..4298
                     /locus_tag="VF589_04725"
     CDS             3657..4298
                     /locus_tag="VF589_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8256914.1"
                     /translation="MAPIGAGGIAEPTDEAFLESIELTPIATVVTDAEAVDNPIVAAN
                     AAFCELTGYSKAEILGRNCRFLAGPKSEPAAREVLRQAIKEQRPALTEITNYRKDGST
                     FRNAVMIAPVLRGDSVAFFIGSQMDTSGTARLETRRMQAETLVSALTPRQRQVLDHMI
                     RGYRNKQIAPLLGIDEKTVKMHRAGLLTRLKAPTSADAIRIGVEAGLQLSDRG"
     gene            complement(4282..5187)
                     /locus_tag="VF589_04730"
     CDS             complement(4282..5187)
                     /locus_tag="VF589_04730"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX8256915.1"
                     /translation="MIDKIRVAVTLGPLMRRTAADIDALLEDALADPPDPRRRLYTAM
                     RYAAVGGGKRLRPLLVLSSASLFGVPRERALRAGLAVECVHAHSLIHDDLPCMDDDDL
                     RRGKPTVHRAFDEATAVLAGDALLALAFELLANAATHPSGDIRAELVGELARAAGAAG
                     MAGGQMLDLLPGEAMDLDTITRLQRLKTGALIGWSVEAGAILGGAAPELRTSLRGYAH
                     CVGLAFQIADDLLDHDGDEAKAGKRLRKDEEQGKATFVTLLGADRARRQAEILVDQAV
                     EHLRAFAGRAQLLEAVAQFAISRDR"
     gene            complement(5190..6185)
                     /gene="ispH"
                     /locus_tag="VF589_04735"
     CDS             complement(5190..6185)
                     /gene="ispH"
                     /locus_tag="VF589_04735"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX8256916.1"
                     /translation="MLELCLPSPAHAFRDGDAADLRPEVQVLLAAPRGFCAGVRRAID
                     AVRDALALHGAPVFVRRPIVHNLTVMRMLAAEGAVFIQELSEAPEGAVVILSAHGVAP
                     IVKEEAERRRLRVYDAVCPLVAKVHREVQKHHQAGRHVVLVGHEGHPEIVGTLGHLPE
                     GAASLVRSAADVAALDLPPDLPVGVAVQTTYSVHEAAEMLAAIKGRFADVATPPRSDI
                     CYATTNRQGAVRAIAARADAVIVAGEDFSSNACRLAELAAAAGCASVQLVSTADALDW
                     ARLRQSRVIGVTAAASTPESVVDGIIDALRARYRLRIEEVAAAEETIMFRRVAVA"
     gene            complement(6249..6659)
                     /locus_tag="VF589_04740"
     CDS             complement(6249..6659)
                     /locus_tag="VF589_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX8256917.1"
                     /translation="MIILMLAAAPLAPPPELEVNVERLRNGRGLVHLCLTRNPAHFPD
                     CGSDPGAVKRSVDARLRTPIRIAGIAPGAWALSVIHDENGNGKLDTMIGIPREGFGFS
                     RNPTIRFGPPRFGQVRFDLGGGIARQTVRMQYLL"
     gene            6719..7879
                     /gene="crtY"
                     /locus_tag="VF589_04745"
     CDS             6719..7879
                     /gene="crtY"
                     /locus_tag="VF589_04745"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HEX8256918.1"
                     /translation="MTAARPRLVIAGGGLAGCLAALALAARRPDVDLLLVEQGDGFGG
                     NHIWSCFDSDVAGEDSWLFELLAARRWPDHEIHFPHRSRILGLGYNSLRSADLDRIMR
                     ETLRPDQYRLGRRIAEVGTQHVLLEGGERIEADGVIDARGASELPGLELGWQKFLGRT
                     YRFDRPHGCARPLIMDARVEQDNGYRFVYLLPFDEARLMAEDTYYSATPVLDLPLLRC
                     RLDSYLSRKGLGPAEVTGEETGVLPVVLAGEVASVWPAAEPPVARLGLRGGFFHPTTG
                     YSLPDAIRNAALLAASSEFGSKALHDLFRGRAAALWDERRFYQKLNRMLFRAAAPNRR
                     YRVLEHFYRLPEPLIARFYAGRLTTLDKLRILSGRPPVPVGRALAALRNRAA"
BASE COUNT         1171 a         2759 c         2690 g         1285 t
ORIGIN      
        1 gcctgcccat ggcggcctga taggcactat tttgaacagt gtctattttt ataattgaac
       61 agtgatcagt ttttcggtat cagctcactc ttccgttaac ccactcacgc tgacgatccg
      121 ctagcgtcac ctccggatcc aggggaagat cgaatgtccg cacccgattt gtcgctgctc
      181 ggcaagcgcc gtttcgctcc gttgttcgcg gtccagttcc tcggcgcgtt caacgacaac
      241 ctcttgaagt tcgccttgct gttcctcgct aatttcggcc tctacgcggc cgagcccgac
      301 aaggcggaga tgctggcgac cgtcgccacc ggcctgttca tcctgcctta tttcctgttc
      361 tccgcgctcg cgggccagct cgccgacgcc tgggacaagg ccaagctggt gcgggcggtg
      421 aagcttgccg agatcggcat catgggactc gcgctggccg gcttttggag cgaatcggtg
      481 ccgctgctgc tcgccagcct gttcctgatg ggcctccatt cgaccctgtt cgggccggtc
      541 aaatattcga tcctgccgca gcatctcgaa tcgcgcgaga tcatgggcgg gaccgggctg
      601 atcgaggccg gcaccttcct cgccatcctc ggcggccagt tgctcggcgg cgtcatcccg
      661 ccctgggagg ccgggctcgt cgcgaccggc ctcgccatcg tcggcttcct cgccagcctc
      721 gccgtgccgc ccgcgcccgc caccgcgccg gggctcagga tcgacttcaa catcttccgc
      781 ggcacctggc acatcctcaa ggcggcgcgc cacggccgcg gcgtttggct gtcgatcctc
      841 ggcatcagct ggttcttcgc ggtcggcgcg gtgctcctgg ccgagttcgc gccgctcgtc
      901 agcggcgtgc tcggcgcccg ccaggaggtc gcgaccctgt tcctgctggt cttctcggtc
      961 agcgtcgctt tgggctcggt gatggtgaac aggctgctcg ccggtgaagt gtcggcgcgc
     1021 tacgtgccgg tcgcggcgct agccctgtcg ggcttcatga tcgacttgtg gctggcgacc
     1081 cgcggctacg agatccagac ggcgggcgcc accatcgccc agttcctggc gagcggcgga
     1141 agctggcgca tcctcatcga cctggcaggc atcgcttttg ccggcggcat gttcatcgtg
     1201 ccgctctacg ccatccttca gacccacagc ccgcccgcgg aacgatcgcg caccatcgcc
     1261 gccaacaaca tcgtcaacgc cgccgtcacc gtcgtcatgg tggccgtgct tgccgctttg
     1321 ctcggctccg gggtggacgt gcccgcgatc atcggcatcc tcggcggcgc gaccttggcg
     1381 gtggccctgg tttcctgctg gctgcttccc gagacggtca tcaagagcct ggtccgcggc
     1441 ctgctgcggc tgctctaccg ggtcgaggtc gagggcgagg agaatatgcc gctgccgggc
     1501 gagcgcgcgg tggtggtggt caaccatgtc tccttcctcg acgggctgct gctcgccgcc
     1561 ttccttccgg gcaagccgac cttcgccgtc cacacccgca tcgccaaagc ctggtgggtg
     1621 cgccccttgc tcggcctgtt cgacgccttt ccagtcgatc cggtcaaccc gatggcggcc
     1681 aaggcgatgg tgcgggcggt caaggaaggc cgcacgttgg tgatcttccc cgaaggccgg
     1741 atcacggtca ccggggcgct gatgaaggtg ttcgacggcc ccggcatggt cgccgacaag
     1801 gccgatgcgc cgatcgtccc ggtccgcatc gacggcgcgc aatattcgcc tttctcgcgg
     1861 ctgcgcggca aggtgcggct gcgtagcttc cctaagatcc ggctgaccat cctgccgccg
     1921 cgccgcttcg ccatcgacgg cacgatgagc gcacgccagc gccgcgcgat cgccggccgc
     1981 cgcctctatg acgaaatgag cgagatgatc ttcgcgacct cgaacaccgg ccgcaccctg
     2041 ttcgaagcct tgctcgacgc ccggcgcatc cacggcggta aggcgccgat cgtcgaagac
     2101 atcaagcgcg agccgctgag ctacaaccgc ctcgtcgccg gcagcatggc gctggggcgg
     2161 ccgctggcac ggctcgcggc gccggacgaa gcggtcggcg tgctgctgcc caatgtcggc
     2221 ggcgtcgcgg tgaccttctt cgcgctccag gcggccgggc gggtaccggc catgctcaac
     2281 tacaccgcgg gcctcaccaa tttgaaagcg gcctgcgcgg cagcggaaat ccgcaccatc
     2341 gtgacggcgc gcgccttcgt cgagcaggcg aagctcgccg acaccatcgc cggcctcgag
     2401 gcaagcgggc tcatcgtcgc ctatctcgag gatatcggcg cgggcatcgg gaaaggcggc
     2461 aagctgcgcg cactcgcggc agcgcggttt gcgcgccaaa tccaccgcgc gaagcgggtt
     2521 tcgcctgacg ccccggccgt gatcctgttc accagcggat cggaggggct gcccaagggc
     2581 gtggtgctga cccaccgcaa tttgctcgcc aattgccagc agctggcggc gcggatcgac
     2641 ttcaacgcct cggacatcgt cctcaacgcg ctcccggtct tccacagctt tgggctgacc
     2701 ggcggcacct tgctgccgat cctgaacggc gttcggacct tgctctatcc gagcccgctc
     2761 cactaccgga tcgtgccggc gctcgcctat gacgcgaacg cgaccatcct gttcggcacc
     2821 gacaccttct tggcgggcta tgcgcggatg gcgcacggct atgatttcta ttcgctgcgc
     2881 tacatcttcg ccggcgccga gcgggtgcgg gacgagacgc gcaggaccta ttcggacaag
     2941 ttcgggctcc gcatcctcga aggctatggc gcgaccgagg cgggcccggt gatcgcggtc
     3001 aacacgccga tgcacttccg cgccggttcg gtcggccggc tgctcccggg tatcaccgcg
     3061 cgcctcgatc cggtgccggg cattgcggag ggcggccggc tggcgatacg ggggcccaac
     3121 atcatggcgg gttacctcaa ggcggatgcg ccgggcctgc tgcagccgcc cgagggcggc
     3181 tggcacgata gcggcgatat cgtcaccatc gacgaagaag gcttcgtcac catccgcgga
     3241 cgcgccaagc gcttcgccaa gatcggcggg gagatggtgt cgctgccggc ggtggaaggt
     3301 tatgccgccg ctgtgtggcc ggaggcggac cacgccgtcg tgacccgccc ggacccgcgc
     3361 aagggcgaac agctcgtcct cttcaccaca cgggccgacg cggacgcggg cgccttgcag
     3421 gcctgggccc gcgccaacgg cgtcaccgag ctgatgatcc cgcgcgacat acgggtgctc
     3481 gacgcgctgc cggtgctggg gaccggcaag ctcgattatg tcgcgatggg cgaggcggcc
     3541 gcgctctccc cccaaggcgg gaagctgcag cctgcggcgg tgtgatgcaa ttgtcagctt
     3601 cgggcccgta atcgtgccat ctcgcggcgc cgcgaggaac cgggtagaag actcccatgg
     3661 caccgattgg cgcaggcggc attgcagagc ccaccgacga ggcattcctc gaatcgatcg
     3721 aactcacccc gatcgccacg gtggtgaccg acgcggaagc ggtcgacaac ccgatcgtgg
     3781 cggcgaatgc ggccttttgc gagctgaccg gctattcgaa ggcggagata ttgggccgca
     3841 actgccggtt cctggcgggg ccgaaatcgg agcccgccgc ccgcgaagtc ctgcgccaag
     3901 cgatcaaaga gcagcgtccc gcgctgaccg agatcaccaa ctatcgcaag gacggcagca
     3961 ccttccgcaa cgccgtgatg atcgctcccg ttctgcgcgg agacagcgtc gccttcttca
     4021 tcggctcgca gatggacacc agcggaaccg cgcggctgga gacccggcgg atgcaggccg
     4081 agactttggt ctccgccttg accccgcggc agcgccaggt cctcgaccat atgatccgcg
     4141 gctaccgcaa caagcagatc gcgccgctgc tcggcatcga cgagaagacg gtgaagatgc
     4201 accgcgcggg cctcctcacc cggctcaagg cgccgacctc ggcggacgcg atccggatcg
     4261 gagtggaagc ggggctgcag ctcagcgatc gcggctgatc gcgaactgcg cgaccgcctc
     4321 gagcaattgc gcccgcccgg cgaaggcgcg gagatgctcg accgcctggt cgaccaatat
     4381 ctccgcctgg cggcgggcgc ggtcggcgcc cagcaaggtg acgaaggtcg ccttgccctg
     4441 ttcctcgtcc ttgcgcagcc ttttgccggc cttggcctcg tcgccgtcat ggtcgagcag
     4501 gtcgtcggcg atctggaagg cgagcccgac gcaatgggca tagccgcgca ggctggtgcg
     4561 aagctcgggc gcggcgccgc cgaggatcgc gcccgcctcg acgctccagc cgatcagcgc
     4621 gccggtcttg aggcgctgga gccgggtgat ggtgtcgagg tccatcgcct cgccgggcag
     4681 caggtcgagc atctggccgc cggccatgcc ggccgcgccc gccgcccgcg cgagctcgcc
     4741 gacgagctcg gcgcggatgt cgccggacgg gtgggtcgcc gcgttcgcca ataattcaaa
     4801 ggcgagcgcc aagagggcgt cgccggcgag gaccgcggtc gcttcgtcga aggcgcggtg
     4861 cacggtcggc ttgccgcgcc gcagatcgtc atcgtccatg cacggcaagt cgtcatggat
     4921 cagcgagtgg gcgtggacgc attcgacggc aagcccggcg cggagcgcgc gctcccgcgg
     4981 cactccgaac aggctcgccg acgacagaac cagcaacggg cgcagccttt tgccgccgcc
     5041 gaccgcggcg tagcgcatcg cggtgtacaa tcgacggcgc gggtcgggcg ggtcggcgag
     5101 cgcgtcctcg agcagcgcgt cgatatcggc ggcggtgcgg cgcatcagcg ggccgagcgt
     5161 caccgcgacc cggatcttgt cgatcatggt catgcgaccg cgacccgcct gaacatgatc
     5221 gtctcctcgg ccgccgccac ttcctcgatc cggagccggt agcgggcgcg aagcgcgtcg
     5281 attatgccgt cgacgacgct ttcgggagtc gaggccgccg cggtgacgcc gataacccgg
     5341 ctttgccgca gccgcgccca atcgagcgca tccgcggtcg agaccagctg caccgacgcg
     5401 cagccggcgg cggcggcgag ttcggcgagc cggcaggcgt tggacgagaa atcctcgccg
     5461 gcgacgatga cggcgtcggc gcgcgcggcg atggcgcgca ccgcgccttg gcgattggtg
     5521 gtggcgtaac agatgtcgct gcgcgggggc gtcgcgacgt cggcgaatcg gcccttgatc
     5581 gccgccagca tttcggccgc ctcgtggacc gaataagtgg tctggacggc gacgccgacc
     5641 gggaggtccg gcggcagatc cagagcggcg acgtccgccg cgcttcgcac caggctggcc
     5701 gcgccctcgg gcagatggcc gagcgtgccg acgatctcgg gatgcccttc gtgcccgacc
     5761 aggacgacgt ggcgcccagc ctgatgatgc ttttgcacct cgcgatggac cttcgccacc
     5821 agcgggcaga ccgcgtcata gacgcgcagc cggcgccgct ccgcctcctc cttgacgatc
     5881 ggagcgactc cgtgggcgga caggatgacg accgcccctt ccggagcctc gctcaattcc
     5941 tggatgaaca cggcaccttc cgcggcgagc atgcgcatca ccgtcagatt gtggacgatc
     6001 ggccggcgga cgaagaccgg ggcgccgtgc agggcgagcg catcgcgcac cgcatcgatc
     6061 gcgcgccgca ctccggcgca gaagccgcgc ggcgcggcca gcagcacctg gacctccggg
     6121 cggaggtcag cggcatcgcc gtcgcgaaac gcgtgcgcgg gactgggcag gcatagctcc
     6181 aacatgtatt ccctgcatcc cgccccattt cagaggttag aggggcggcc ccggcgaatc
     6241 cacatggact aaagcaggta ttgcatgcgc accgtctggc gcgcgatgcc gccgccaagg
     6301 tcgaagcgga cctggccgaa gcgcggcgga ccgaaacgga tggtcggatt gcgcgaaaag
     6361 ccgaagccct cacgaggtat tcctatcatc gtatcgagct tgccattgcc attctcgtca
     6421 tggatcaccg acagggccca ggcccccggg gcgatgccgg cgatgcggat cggcgtgcgc
     6481 agccgcgcgt cgacgctgcg tttgacggcg ccggggtcgc tcccgcaatc ggggaaatgc
     6541 gcgggattgc gggtcaggca aaggtgaacc agcccgcggc cgttccgaag gcgctcgaca
     6601 ttgacctcca gctcaggcgg cggggcgagc ggtgcggcgg caagcatgag gatgatcata
     6661 ggcttggcta caggccaacg ggcggggttg ccatctggta tttaggcggg gtggtcgctt
     6721 gaccgccgcc cgcccccgtc tcgtcatcgc cggcggcggc ctcgccggct gccttgccgc
     6781 gctggcgctg gcggcgcggc gtccggacgt tgacctgctg ctggtcgagc agggcgacgg
     6841 cttcggcggc aaccacatct ggtcctgctt cgacagcgat gtcgcgggcg aggacagctg
     6901 gctgttcgag ctcctggcgg cccgtcgctg gcccgaccac gaaatccact tcccgcaccg
     6961 gagccgaatc ctcggactgg gctataacag cctccgctca gccgatctcg accggatcat
     7021 gcgcgagacg ctgcggcccg accaatatcg gctcggccgg cgcattgccg aggttgggac
     7081 ccaacatgtg ctgctcgagg gcggcgagcg gatcgaggcc gacggcgtga tcgacgcccg
     7141 cggcgcctcc gaactgccgg gcctcgagct tggctggcag aaattcctcg gccgcaccta
     7201 ccgcttcgac cggccgcatg gctgcgcccg gccgctgatc atggacgccc gggtggagca
     7261 ggataacggc taccgcttcg tctacctgct gccgttcgac gaggcccggc tgatggccga
     7321 ggatacttat tattcggcga ccccggtgct cgacctgccg ctgctgcgat gccggctgga
     7381 cagctatctg agccgcaagg gattggggcc ggcggaagtg actggcgagg agacgggcgt
     7441 gctccccgtg gtgctcgccg gcgaggtggc gagcgtgtgg ccggccgccg agcccccggt
     7501 ggcgcggctg ggcctcaggg gcggcttctt ccatccgacc accggctatt cgctgcccga
     7561 cgcgatccgc aacgccgccc tgctcgccgc gtcgtccgaa ttcggcagca aggcgcttca
     7621 cgacctcttc cgcggccgcg cggcggcttt gtgggacgag cggcgcttct accagaagct
     7681 caaccgcatg ctgttcaggg cggcggcgcc caaccggcgc taccgcgtgc tcgaacattt
     7741 ctaccggctg ccggagccgc tcatcgcccg cttctatgcc ggccggctga cgacgctcga
     7801 caagctgcgc atcctttccg gccgcccgcc ggtgccggtc ggccgcgcgc tcgcggcgct
     7861 tcggaaccgc gccgcatgag ccggcgcgcg gcggtgatcg gctcc
//
LOCUS       DASVRK010000124         9673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_458350, whole genome shotgun sequence.
ACCESSION   DASVRK010000124 DASVRK010000000
VERSION     DASVRK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9673
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458350"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VF589_04750"
     CDS             <1..613
                     /locus_tag="VF589_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8256919.1"
                     /translation="DAGSCSSRVVFNAPSAHTMGLEFELFASPVEGLDLSLTGSLIEA
                     EFDSTILKANGDILEGIAEGNRLPSVPKFQISANASYSWPIDANGTDAFVAASFQHIG
                     SRFTQPGDQENNPRTFVHGLPFGGAPATASTTLDLKLPDYQLVNLSAGVEMDNGIDVI
                     AYVNNVFDENALLSFDRERGGRARLGFSTNQPRTFGVTVRKEF"
     gene            663..1907
                     /locus_tag="VF589_04755"
     CDS             663..1907
                     /locus_tag="VF589_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8256920.1"
                     /translation="MRAITRRSALLVGAGAAATAAAPRAARARGDAGRTVVVGAGAFG
                     AWTAEHLRRAGHKVMLLDAWGPAHSRASSGGESRMTRGAYGPDEVYARMARDSLIEWR
                     ALSGRAGLPIFHETGVLFFFPTLEPYAEQTMAVHRRLGFDTMLMEPKQLRERFPQMNW
                     DGVAIGIYEPGFGALMARRAVQTLVAEYVPQGGLYERAAVAPPTGAGDRLTHVTTAKG
                     DRIEADRFVFACGPWLPNLFPDLLGTRIFPTRQEVFFFAPAAGDERFLPGRLPGWADF
                     NGGDIYYGFPDLEGRGFKIAHDKHGPPIDPDRGDRGHSEAALADVRGYMKRRFPALAD
                     RPMTEARVCQYENSANGDLLIDRHPRWANVVLVGAGSGHGFKHGPEVGRYAAALTAGT
                     LERTEPRFSLATKAETQARAVH"
     gene            2046..4268
                     /locus_tag="VF589_04760"
     CDS             2046..4268
                     /locus_tag="VF589_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256921.1"
                     /translation="MAITISYALHYETSLDTGGLTSAIDAVGLEGGGLAMIGNEGAAS
                     RSLFLDSDGFQSSGGPYSGSGGAVAQLANGDVVLVRDSGNEFVYSVRTADDRVVRGTY
                     PLGGTSHVSHVDVAALDGGGFVIAYEQKVGEGDSDIRLRILDPNGSGAAPIITVAGTS
                     DLEQGVSVAGLVGGGIAVAWHRTVGAETEMWLAVYESNGDVRFAPRLQGFHGTINRNA
                     SLVALPDGGFALAYEGSGVNGNPHTDIFVALFDPHSAYAGRKHVLSHEHDAEEVDDGA
                     PSATLLSNGMIAVGWLRTSPDGATDPHFELVDPHSALELAALDLPGDGMSSELAVAGM
                     SLGRLAAFHTNAATGDTTGGLLEAVRTTRGDEADNVLSGDSLRDIIFGGGGADTISGG
                     GNADALDGGEGDDTFRFQAAGAAAGATVDGGSGSDLLHIVDGADLTAVGLVSIERMLL
                     GGDSGGSPVAKVVIGAAQIGAGLASNLEVSGGDSADWFQVVLGGAASVDLSQMTFTNF
                     DPGADRVTILGDGDAETIMGSALGDDIRGGGGNDRLSGGGGVDRLRGGLGNDLYTVDC
                     AGEAIETSGQGTDIVRSAIGYTLGDHIERLELIGAGHINGRGNALGNGINGNAGDNIL
                     NGLAGRDMLTGGAGADDFAFTTALDAPGNVDLVADFEVGIDRIRLDDAIFGGLTDAAL
                     ATAFHVGNAAADASHRIIYNAETGALYFDPDGAGGARQIRFATLDAGLALSAADFIIV
                     "
     gene            complement(4285..5271)
                     /locus_tag="VF589_04765"
     CDS             complement(4285..5271)
                     /locus_tag="VF589_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8256922.1"
                     /translation="MKSIAYRSSAHRLHPRDLLVVGMLALSASCVPAGSAVAPARAGL
                     TLTLPPLSTLETPPAPESVMAPALPAAKAVALNAAIPFAAAPSPAARPSLFRAATPLD
                     QMRAIDCLAQAIYYEAASETEAGQRAVAQVVLNRVRHPAYPNSVCGVVYQGPMRAGGG
                     CQFTFTCDGSLVRRPSATGWSRARLLASEALAGKVFAPVGHATHYHTFAVHPHWAPRL
                     AKIAAIGAHIFYRFPNGWGEPAAFRAPYAASEPIPKPAATRLTFASAAPRLEVPPAAL
                     FEPLRIAAPAGRAALAIADDTVTAPAAQDNLPQSTIRPEFANSGRPREVPLD"
     gene            complement(5796..7517)
                     /locus_tag="VF589_04770"
     CDS             complement(5796..7517)
                     /locus_tag="VF589_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8256923.1"
                     /translation="MPSGADQADLDVFLVIAPAGRDAQVIGELLASAQLACRYDHDGD
                     QLLEALIAGEAAGAIVTDDALARIGTARLREAIDRQPPWSDFPFVLLTRRGETRQGAR
                     SIEELVNVTVLERPLHPASLVSAARAARRGRSRQRLAARHLAEREAARAELRELADTL
                     EAKVEERTRDLAAANDRLTAEIGERERAEARLVQAQKMEAVGQLTGGIAHDFNNLLTA
                     VVGSLDLLLRRTDDEKLRRLAGNALQAAERGAQLTAQLLAFSRRQRLSPAPLQPNEIV
                     SGMGDMLARTIGPHVRVETRLDPHLWKALADPTQLEVMILNLAINARDAMPGGGRLTV
                     STRNVESVPAPLAAELPPGEYVAIAVSDTGTGMTPGVLARAFEPFFTTKEQGKGTGLG
                     LAQLYGFARQSGGAARIDSREGEGTTVTIYLPRTREQAAAPAATLLEARPRERARILV
                     VDDDDDVRMVAAALVEEIGYEVVAVGSGEAALKEIEAGQFALLLTDVAMPGLTGVDLA
                     RRVREFSPDLPIIFASGYADVQTFGEELTDEILLKKPYRIAEVAARISAALDERRRGD
                     NVVDLRR"
     gene            complement(7501..9012)
                     /locus_tag="VF589_04775"
     CDS             complement(7501..9012)
                     /locus_tag="VF589_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX8256924.1"
                     /translation="MSGERELLEDAGPVSSGSAALDYILRGGYAANRVHLLEGEPGAG
                     KTTLGLQFLLDGAAKHERCLYITLSESRNELLHVATTHGWDLGGIDIFELVPPELSLD
                     TEREQSIVYASDLELGETVQMVMDEVERMAPARIVFDSLSEIRLLAQGPLRFRRQVLA
                     LKHYFAKHNCTVLFLDDLTQTENDLSLHSLAHGVVRLDQVAVAYGAERRRLRVYKMRG
                     RAFRGGFHDFAIRTGGLDIFPRLVASSYPDGGRAEKVALSGVEALDRLTGNGLDYGTS
                     TLIIGPSGAGKSTLALQYAYAGLARGEKAFFVSFDETEAIFLRRARGLGMDIDDYQQK
                     GLFTFRQVDPAELSPGELTSILRQQVEEGAQIVVLDSLSGYQHAMPEEQYMLLQMHEI
                     VTYLNQQGILTLLVLAQSGMVGHMQSPIDLTYLADAVMLLRFFEARGEIRRAISVLKK
                     RTGAHESTLRELKIDAGGIRVGEKLDGFRGVLTGVPTIEGGVNLLRDRGAERG"
     gene            9210..>9673
                     /locus_tag="VF589_04780"
     CDS             9210..>9673
                     /locus_tag="VF589_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX8256925.1"
                     /translation="MADLYSQLGVARSASEAEIKKAYRKLAKELHPDRNKDNPQATDR
                     FSKVTRAYDILTDKDKRARYDRGEIDEDGNPRMPFGYGGGAGGPRPGPGGFRGPNGEE
                     FTADTADLSDLFDGLFSGGQRRSGGGGGGPFGGFRGFGRRSAPPQKGADVAYR"
BASE COUNT         1534 a         3267 c         3429 g         1443 t
ORIGIN      
        1 ggacgccggc tcctgctcgt cgcgcgtcgt gttcaacgcg ccgagcgcac atacgatggg
       61 cctcgaattc gaactgttcg cctcgccggt ggaaggcctg gacctgtcgc tgaccggcag
      121 cctcatcgag gccgagttcg attcgaccat cctcaaggcg aatggcgaca tcctcgaagg
      181 cattgccgag ggcaaccggc tgccgtcggt gccgaaattc cagatttcgg ccaatgccag
      241 ctatagctgg ccgatcgatg ccaacggcac cgacgcgttc gtggcggcct ccttccagca
      301 tatcggcagc cgcttcaccc agcccggcga ccaggagaac aacccgcgca cattcgtcca
      361 cggcctgccg ttcggcgggg cgccggcgac cgcctcgacc acgctcgatc tgaagctgcc
      421 cgactaccag ctggtcaacc tcagcgccgg cgtcgagatg gacaacggca tcgacgtgat
      481 cgcctatgtc aacaatgtgt tcgacgagaa tgctttgctg tcgttcgacc gggagcgcgg
      541 cggccgcgcc cggctgggct tctcgaccaa ccagccgcgc accttcggcg tcaccgtccg
      601 caaggaattc tgatggcggt ggccggcccg gcgtcggacc ggctccgctc caggtgagag
      661 ggatgcgcgc aatcacccgc cgatccgcgt tgctggttgg ggccggagcc gcggcgacgg
      721 ccgccgcccc cagggccgca cgcgcccgcg gcgacgccgg ccgcaccgtc gtcgtcggag
      781 cgggcgcgtt cggggcgtgg acggcggagc atctccgccg cgccggccac aaggtgatgc
      841 tgctcgacgc ctgggggccc gcgcacagcc gcgcctcgtc gggcggggaa tcgcgcatga
      901 cccgcggcgc ctacggcccc gacgaagtct atgcgcggat ggcgcgggat tcgctgatcg
      961 aatggcgggc gctgtcgggc cgggcggggc tgccgatctt ccacgaaacc ggcgtgctgt
     1021 tcttcttccc gacgctcgaa ccttatgccg agcagacgat ggcggtgcac cgcaggctcg
     1081 gcttcgacac gatgctgatg gagcccaagc agctgcgcga gcgcttcccg cagatgaact
     1141 gggacggcgt cgcgatcggc atttacgagc ccggcttcgg cgcgctgatg gcgcggcggg
     1201 cggtccagac tttggtcgcc gaatatgtgc cgcagggcgg gctttacgag cgcgccgcgg
     1261 tggcgccgcc gacgggcgcc ggcgatcggc tcacccatgt gacgaccgcc aaaggcgacc
     1321 ggatcgaggc cgaccgcttc gtcttcgcct gcgggccgtg gctgcccaac ctgttccccg
     1381 atctgctcgg cacgcgcatc ttcccgaccc ggcaggaagt gttcttcttc gcgcccgcgg
     1441 ccggcgacga gcgtttcctt cccggccgcc tgccgggctg ggccgacttc aacggcggcg
     1501 acatctatta cggctttccc gacctcgaag ggcgcggctt caagatcgcg cacgacaagc
     1561 acgggccgcc gatcgatccg gaccgcggcg accgcggcca ttcggaggcc gctctggccg
     1621 acgtgcgcgg ctatatgaag cggcgcttcc cggcgctggc cgaccggccg atgaccgaag
     1681 cgcgggtctg ccaatatgag aatagcgcca atggcgactt gctgatcgac cgccacccgc
     1741 ggtgggcgaa cgtcgtgctg gtcggcgccg gatcgggcca cggcttcaag cacgggccgg
     1801 aggtcggccg ctacgccgcg gcgctgacgg cggggacgct cgagcggacg gagccgcgct
     1861 tttcgctcgc caccaaggcg gagacgcagg cgcgcgcggt gcattgaggg gcgcgcggct
     1921 tcgtcgcagc aagcgccaaa cctggtggca cagccgccgc cgtcatgagc gaaaaagtaa
     1981 tctagccagt ggtaacgccg acgagttgat gacgggagcg accgctcccg caccgggggg
     2041 acgccatggc aatcacgata agctacgcgc tgcactatga gacaagcctc gacacgggcg
     2101 gcctgacctc cgcgatcgac gcggtcgggc tcgagggtgg cgggctcgcc atgatcggca
     2161 acgaaggggc ggcatcgcgg tcgctattcc tcgacagcga cggcttccaa tcgagcgggg
     2221 gcccctattc cgggagcggc ggcgcggtcg cgcagctcgc caacggcgac gtcgtgctgg
     2281 tccgcgactc cggcaacgag ttcgtctatt cggtgaggac cgcggacgac cgggtggtgc
     2341 ggggcaccta cccgctcggc gggacgagcc atgtttccca cgtcgacgtg gcggcgctcg
     2401 acggcggcgg cttcgtgatc gcctatgagc aaaaggtggg cgagggcgac agcgacatcc
     2461 gcctgaggat cctggatccg aacggttcgg gcgcggcgcc gatcatcacc gtcgccggaa
     2521 cgagtgacct tgaacagggc gtcagcgtgg cgggcctggt cggcggcgga atcgccgtcg
     2581 catggcaccg caccgtcggc gccgagaccg aaatgtggct cgccgtctat gaaagcaatg
     2641 gcgacgtccg cttcgccccc aggctgcaag gctttcacgg gacgatcaat cgcaacgcca
     2701 gcctggtcgc gctcccggac ggcggcttcg cccttgccta tgaaggcagc ggcgtgaacg
     2761 gcaaccccca taccgacatc tttgtggcgc tcttcgatcc tcacagcgcc tacgcgggcc
     2821 gcaagcacgt cctttcccac gaacatgatg ccgaggaggt cgacgacggc gcgccttcgg
     2881 cgacattgct gtcgaacggg atgatcgcgg tgggctggct gaggactagt cccgacggcg
     2941 cgaccgatcc tcatttcgaa ctggtcgacc cgcatagcgc gctggagctg gccgccctcg
     3001 acctcccagg cgacggcatg agctccgagc tggcggtcgc tggcatgagc ctcggccgcc
     3061 tcgccgcctt ccacaccaac gccgcgaccg gcgacacgac cggcggcctg ctggaggcgg
     3121 tgcggacgac ccggggcgac gaggccgaca acgtcctgtc gggcgacagc ctgcgcgaca
     3181 tcatcttcgg cggcggcggc gcggacacga tctccggcgg cggcaatgcc gacgcactcg
     3241 atggcggcga gggtgacgac acgttccggt tccaagccgc cggcgccgcg gccggcgcga
     3301 ccgtcgacgg cggcagcggc agcgatctcc tgcacatcgt ggatggcgcc gacctcaccg
     3361 ccgtcggcct ggtctcgatc gagcggatgc tgctcggcgg cgactccggg gggagccctg
     3421 ttgccaaggt cgtgatcggg gcggcccaga tcggcgccgg acttgcctcc aacctcgaag
     3481 tctcgggcgg cgattcggcc gactggttcc aggtggtact gggcggcgcc gcgtccgtgg
     3541 acctgtcgca gatgacgttc accaatttcg acccgggagc cgatcgcgtc acgatcctcg
     3601 gcgacggcga tgccgagacc atcatgggct cggcgctggg cgacgacata cgcggcggcg
     3661 gcggcaacga ccggctcagc ggcggcggcg gcgtcgaccg gctgcgcggc ggcctcggca
     3721 acgatctcta cacggtcgat tgcgccgggg aggcgatcga aacgtccggc cagggaaccg
     3781 acatcgtgcg ctcggcgata ggctatacgc tcggcgacca tatcgagcgg ctggagctga
     3841 tcggcgcggg ccacatcaac ggcaggggca atgcgctcgg caacgggatc aacggcaatg
     3901 ccggcgacaa tattctcaac ggcctcgccg gccgggacat gctgaccggc ggtgcgggcg
     3961 cggacgattt cgccttcacg accgcgctcg atgcgcccgg caacgtcgac ctggtcgcgg
     4021 atttcgaagt gggcatcgac cgcatccggc tcgacgatgc gatcttcggg ggcctcaccg
     4081 atgccgccct cgccaccgcc ttccatgtcg gcaacgccgc cgccgacgcc tcgcaccgga
     4141 tcatctacaa tgccgagacc ggcgcccttt atttcgatcc ggacggtgcc ggcggcgcgc
     4201 gccagatccg gttcgcgacg ctcgatgcgg ggctggcgct gagcgccgcc gacttcatca
     4261 tcgtctgagc ccgcgcgcgg gcgctcagtc gagcggcact tcgcgcgggc ggccgctatt
     4321 ggcgaattcc gggcggatcg tggattgcgg aagattgtcc tgggccgccg gagccgtcac
     4381 ggtgtcgtcg gcgatggcga gcgcggcgcg cccggccggc gcggcgatcc ggagcggctc
     4441 gaacaaagcg gcgggcggga cttccagccg gggcgccgcc gaagcgaagg tcaggcgcgt
     4501 cgccgccggc tttgggatcg gctccgacgc ggcataaggc gcgcggaagg cggcgggctc
     4561 gccccagcca ttggggaagc gatagaagat gtgcgcgccg atggcggcga tcttggcgag
     4621 ccgcggcgcc caatgcgggt gcaccgcgaa cgtatgataa tgggtggcgt ggcccaccgg
     4681 cgcgaacacc ttgccggcga gcgcttcgga ggccagcagg cgggcgcggc tccagccggt
     4741 cgccgagggg cggcggacca gcgaaccgtc gcaagtgaaa gtgaactggc agccgccgcc
     4801 ggcgcgcatc ggcccctggt agacgacgcc gcagacgctg ttcggataag cgggatggcg
     4861 gacccggttc agcaccacct gagcgacggc gcgctggccg gcttcggtct cggaggcggc
     4921 ttcgtaataa atcgcttggg caaggcaatc gatcgcgcgc atctgatcga gcggggtggc
     4981 cgcgcggaac agcgacggcc gcgccgccgg actgggggcg gcggcgaagg ggatggcggc
     5041 gttgagcgcg acggccttgg cggcgggcag agcgggcgcc atcaccgatt cgggggcggg
     5101 cggcgtctcc agggtcgaga gcggcggcag cgtcaaggtg aggccggcgc gggcgggagc
     5161 aaccgcgctg ccggcgggaa cgcaggaggc ggacaaggcc agcatgccga cgacgagcag
     5221 gtcgcgcggg tgcaggcggt gcgcgctgga gcggtaagcg attgacttca tactcttccg
     5281 acagcctttc ggcccgctcg aggagcgagg tcggcagacg ctatgcgcag tttcgagcct
     5341 ttgtcgcgcc cggatttcgg cccgtcccgg aatggcacga agtggcggga ggatgtgaaa
     5401 tcggttcaag cggggatgcg tagaccggga ttggttcctg ttggcttaac tccgctcatc
     5461 ctgaggggcc gtcgagcaaa gtcgaagcgg cctctcgaag gacgcacact tccgctcatc
     5521 ctgaggagcc ggtgagcctg ccgaaccatg aacaggccgg attgcccccg gcaatcctga
     5581 aacatgccgg gggcatgttt caccttttca ttctcgaagg acgcacccag gtccttcgag
     5641 acgggccttc gccaaggctc agtccctcct caggatgagc ggggttcgac aagctcaccg
     5701 ctcctcagga taagcggcaa tggggatcga tcccccgcta ccggcggagg tacccctacc
     5761 ggcggaggtc cccgctaccg ccggaggtcc ccccgctacc gccgaagatc gacgacattg
     5821 tcgccgcggc gacgctcgtc gagggcggcg ctgatgcggg ccgccacttc ggcgatgcgg
     5881 tagggcttct tcagcaatat ctcgtcggtc agctcctcgc cgaacgtctg gacgtcggca
     5941 tagccgctgg cgaagataat cgggaggtcc ggggaaaact cgcgcacccg gcgggcgagg
     6001 tcgaccccgg tcagccccgg catggcgacg tcggtgagga gaagcgcgaa ttggccggcc
     6061 tcgatctcct tgagcgccgc ctcaccgctg ccgaccgcca ccacctcata gccgatctcc
     6121 tcgaccaaag cggccgcgac catgcggaca tcgtcgtcgt cgtcgacgac caggatgcgg
     6181 gcgcgctcgc gcggcctcgc ctcgagcagg gtggcggccg gagcggcggc ctgttcgcgg
     6241 gtgcgcggca gataaatggt gacggtggtg ccctcgccct cgcggctgtc gatccgcgcg
     6301 gcgccgcccg actggcgggc gaagccgtaa agctgggcaa ggccgaggcc ggtgcccttg
     6361 ccctgctcct tggtcgtgaa gaagggttcg aatgcgcgcg ccagcacgcc gggcgtcatg
     6421 ccggtgccgg tgtccgagac cgcgatggcg acatattcgc cgggcgggag ctccgccgcc
     6481 agcggggccg gcacggattc gacgttgcgg gtgctgacgg tcagccggcc gccgccgggc
     6541 atggcgtcgc gcgcgttgat cgcgaggttg aggatcatca cttcgagctg ggtcgggtcg
     6601 gccagcgcct tccagaggtg cggatcgagc cgcgtctcga cccgcacgtg ggggccgatc
     6661 gtgcgggcca gcatgtcgcc catgcccgac acgatctcgt tcggctggag cggcgccggc
     6721 gacagccgct ggcggcgcga gaaagcgagc aattgggcgg tgagctgggc gccgcgttcg
     6781 gccgcctgca gcgcattccc ggcgaggcgg cgcagcttct cgtcgtcggt gcggcgcagc
     6841 agcagatcga gcgagccgac gacggcggtg agcaggttgt tgaaatcgtg ggcgataccg
     6901 ccggtgagct ggccgaccgc ttccatcttc tgggcctgga ccagccgcgc ctcggcgcgc
     6961 tcgcgctcgc caatctcggc ggtcagccgg tcgttggcgg cggcgaggtc gcgggtgcgc
     7021 tcctcgacct tggcctcgag cgtgtcggcg agctcgcgaa gctcggcccg ggccgcctcg
     7081 cgctcggcca aatgccgcgc cgccagccgc tggcgcgagc ggccgcgccg ggcggcccgt
     7141 gcggcgctga cgagcgaggc gggatgaagc ggccgttcga ggacggtgac gttgaccagc
     7201 tcctcgatcg agcgcgcgcc ctggcgggtc tcgccgcggc gggtgagcag cacgaacggg
     7261 aagtccgacc agggcggctg ccggtcgatc gcctcgcgca ggcgcgccgt gccgatccgc
     7321 gccagcgcgt cgtcggtcac gatcgcgccc gccgcctcgc cggcgatcaa cgcctcgagc
     7381 aactggtcgc catcgtgatc gtaacggcag gcgagctgcg cgctcgccag caattcgccg
     7441 atcacctggg cgtcccgccc ggccggcgcg atcaccagga agacgtccag atcggcttgg
     7501 tcagccccgc tcggcaccgc ggtcccgaag caaattcacg cctccttcta tggtgggcac
     7561 tccggtgaga acgccgcgga acccatcgag tttctcgccg acgcgaatgc cgccggcgtc
     7621 gatcttgagc tcgcgcaacg tcgattcgtg cgcgccggtg cgcttcttca gcaccgagat
     7681 ggcgcggcga atctccccgc gagcctcgaa aaagcgcagc agcatcaccg catcggcgag
     7741 gtaggtgaga tcgatcggcg actgcatgtg gccgaccatc ccgctctgcg ccagcaccag
     7801 caaggtgagg atcccctgct gattgaggta ggtcacgatc tcgtgcatct ggagcagcat
     7861 atattgctcc tccggcatcg catgctgata gccggagagc gaatccagga cgacgatctg
     7921 agcgccctcc tcgacctgct ggcgcaggat cgaggtcagc tctccggggg aaagctcggc
     7981 cggatcgacc tgccgaaacg tgaacaggcc cttttgctga tagtcgtcaa tgtccatgcc
     8041 gaggccgcgc gcccggcgca ggaagatggc ctcggtctcg tcgaagctga cgaagaaggc
     8101 cttttctccc cgcgcgaggc cggcataggc atattgcagc gccagcgtcg acttgcccgc
     8161 cccggacggg ccgatgatca gcgtcgaggt gccataatcg aggccgttcc cggtcagccg
     8221 gtcgagcgct tcgaccccgc tcagcgccac cttctcggcg cggccgccgt ccgggtagga
     8281 gctcgccacc agccgcggga agatatcgag cccgccggtg cggatggcga aatcgtggaa
     8341 gccgccgcgg aaggcgcggc cgcgcatctt gtagacgcgc aggcgccggc gctcggcgcc
     8401 gtaggcgacc gcgacctgat cgagccggac gacgccgtgg gcgaggctgt gcaggctgag
     8461 gtcgttctcg gtttgggtga gatcgtcgag gaacaggacg gtgcaattat gcttggcgaa
     8521 ataatgtttg agcgccagca cctgcctgcg aaagcggagc ggcccctggg cgagcagccg
     8581 gatctcggac aggctgtcga acacgatccg cgccggcgcc atccgctcga cctcgtccat
     8641 caccatctgc acggtctcgc cgagctcgag gtcggaggcg tagacgatcg attgctcgcg
     8701 ctcggtgtcg aggctcagct cgggcggcac cagctcgaaa atgtcgatcc cgccaaggtc
     8761 ccagccatgg gtggtggcga cgtggagcag ctcgttccgg ctctccgaca gggtgatata
     8821 gaggcagcgt tcgtgctttg cggcgccatc gagcaggaat tgcagcccga gcgtggtctt
     8881 gcccgcaccg ggctcgccct cgaggaggtg gacgcggttt gcggcatagc cgccgcgcag
     8941 gatgtaatcg agcgcagccg agcccgacga taccggtccc gcatcctcca gcaattcacg
     9001 ctcacccgac attcggccgt ctccttccgg tccgcctctg cccgatccgc gtccggcgtg
     9061 caatttggat caagtcctgg tgctgcgatc ccggtcgcgg aaatcgtccc gccggagact
     9121 tgccacacag gacggccaca cccacctgca acacagttgc cgcgaggcaa cagtggacat
     9181 ttgggagtgg ctggagaacg aagcgcgata tggcggatct ctattcacaa ctgggcgtgg
     9241 cgcggagcgc aagcgaagcc gagatcaaga aggcgtatcg caagctcgcc aaggagctgc
     9301 atccggaccg caacaaggac aatccgcagg cgaccgaccg cttctccaaa gtcacccgcg
     9361 cctacgacat cctcaccgac aaggataagc gcgcccgcta cgatcgcggc gagatcgacg
     9421 aggacggcaa tccgcggatg ccgttcggct atggcggcgg tgcgggcggc ccccgccccg
     9481 gccccggcgg cttccgcggc cccaacggcg aggaattcac cgccgacacc gcggatctat
     9541 ctgacctgtt cgatggcctg ttcagcggcg gccagcggcg cagcgggggt ggcggcggcg
     9601 gaccgttcgg cggcttccga gggttcggaa ggcgatcggc tcctccgcag aagggcgcag
     9661 acgtcgccta ccg
//
LOCUS       DASVRK010000125         3906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_209061, whole genome shotgun sequence.
ACCESSION   DASVRK010000125 DASVRK010000000
VERSION     DASVRK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209061"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2958
                     /locus_tag="VF589_04785"
     CDS             <1..2958
                     /locus_tag="VF589_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015474 - autotransporter activity
                     [Evidence IEA];
                     GO_process: GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX8256926.1"
                     /translation="SIKGEGDYVFGVNSFSGNYVDGEILSGNTKIEVGTIEISGDHAA
                     GVVATSYGSLEVKVDDLKIDGNESGGVFASAIGNVSVEVGDAVFTGDNNGAIVAFSYG
                     EASVKLGTLTAENGGGVVAFGTEGAYASADKIAISGDFQNGIQSVSMFGDATLKVGEV
                     STNGFFAVAVNGDSGQGDVRIEAGKITTAGELAMGIRAFGRTTSIELTGPLSTSGDWA
                     FGILNASMHGDATVRASGPISTTGEGAVGAWITGRYGTADIVVDSVSTSGDDARGIWA
                     TGEDGQVKLVAEEVSTSGANADGIHARTRYVEVFLGQPPVPDPVEFTGDIDIEAGTVS
                     VTGAGSIGISARGLGRVDIDVGSVSAVHSYGVDVNMIGEASLRIGEQAISKRATAVRM
                     EAADIDIAVDEGALVRGAEHGIVIRAEGERCVAPNPKDGSLSPCPNPGDDEFHDPVPV
                     PGPGGAATIVNAGTIRGGTGYAVKVEKGALTLRNSGRIVGAMLLADGDDLIENEGTFD
                     VRADSDFGAGDDRFVNSGVVKLGTTSAPATYRFLGLERFDNQGLVDLRNTAVGDVFSL
                     SGDYAGSGDATLALDVDLANNKADTFVVEGAATGETQIVLDLTPGTARLTSESGITLV
                     ETGEDSNSDAFVLAESSQDIGFIRYGLNYDAAARRYALTGVASAGAYRQLNALEGAEN
                     LWHVSADAWRANAAAERDARFAGADGPRRGTLWAQALGGRISRDEDTDEAGIAGGETV
                     DFAYEQSRIGGQLGVDFAGYDRNDSGLRIGLTAGYIDSELESRAAAEKLDMETFNVGA
                     YASFTSGRLFAGALLKHDWHDIGFDSDADGFEGDADGRTWGVEGEIGARFGGGGFFVE
                     PVASLAWTSTSLDDLQALGQSLDFRGADGLRGKLGARIGGRSDVGNGDALVFHAAAKA
                     VHDFGGDYRLDLNSSGAAQELSSKRIGTFGEGLIGASYIWRGGFEAFVEGQSELGSEY
                     DGLGGRIGFRLRL"
     gene            3047..>3906
                     /locus_tag="VF589_04790"
     CDS             3047..>3906
                     /locus_tag="VF589_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HEX8256927.1"
                     /translation="MTKPTTAAPDITASRGLSRWLKSRHTSFAFSSYQTGQLFLVGSQ
                     ADGTVSVNQHYFQRAMGLAWRPGRLYLASQYQLWRLENVLRPGELANRAFDLLLVPRN
                     AQTTGDLDMHEIAVDDAGRVLFVNTSYSCLATLDLRNSFRPIWKPPFISRLAAEDRCH
                     LNGLAVGPRGEPRYVTAVSRSDVVNGWRERRAEGGVLIDVATGGTVTDQLSMPHSPRL
                     VGDDLYLLDSGRGFLVRIDPKDGSRTDIAFCPGFLRGLSIHAGHALVTVSKPREATFG
                     GLALAGELAAR"
BASE COUNT          639 a         1377 c         1308 g          582 t
ORIGIN      
        1 agcatcaagg gcgaaggcga ctatgtgttc ggggtcaaca gcttcagcgg caattatgtc
       61 gacggcgaga tattgagcgg caacacgaag atcgaggtcg gcaccatcga gatcagcggc
      121 gaccatgccg ccggcgtggt cgcgaccagc tatgggtcac tcgaggtcaa ggtcgacgac
      181 ctcaagatcg atggaaatga aagcggcggc gtgttcgcct cggcgatcgg gaacgtcagc
      241 gtcgaggtcg gcgatgccgt cttcaccggc gacaataatg gcgcgatcgt cgccttcagc
      301 tatggtgagg cgagcgtgaa gctcggcacg ctcaccgccg agaatggcgg cggcgtcgtc
      361 gcgttcggga ccgagggcgc ctatgcgagc gccgacaaga tcgcgatcag cggcgacttc
      421 cagaacggca tccagagcgt ttccatgttc ggcgatgcga cgctgaaagt gggcgaggtc
      481 agcaccaacg gctttttcgc ggtcgcggtg aacggcgatt ccggccaggg cgacgtccgc
      541 atcgaggccg ggaagatcac caccgccggc gagctcgcca tgggcatccg cgccttcggt
      601 cgcaccacct cgatcgagct caccggcccc ctctccacat cgggcgactg ggcgttcggc
      661 atcctcaatg cgtccatgca tggcgacgca acggtccgcg cctcgggacc gatctcgacc
      721 acgggcgagg gtgcggtcgg cgcgtggatt accggccgct acggcaccgc cgacatcgtc
      781 gtcgacagcg tctccacctc cggcgacgac gcgcgcggca tctgggcgac cggcgaggac
      841 ggccaggtca agctcgtcgc cgaggaggtc agcaccagcg gcgccaatgc cgacggcatc
      901 cacgcccgca cccgctatgt cgaagtcttc ctcggccagc cgcccgtccc cgacccggtc
      961 gagttcaccg gcgatatcga catcgaggcc gggacggtca gcgtcaccgg cgccggctcg
     1021 atcggcatca gcgcgcgcgg cctcggccgg gtcgatatcg atgtcggctc ggtcagcgcc
     1081 gtccacagct acggcgtcga cgtgaacatg atcggcgagg cctcgctccg gatcggggag
     1141 caggcgatct cgaagcgggc aaccgccgtc cgcatggaag ccgcggacat cgacatcgcg
     1201 gtcgatgagg gcgctctggt gcgcggcgcg gagcatggca tcgtgatccg cgccgaaggc
     1261 gagcgctgcg tggcgcccaa tcccaaggac gggagcttga gcccctgccc caatcccggc
     1321 gacgacgaat tccacgatcc cgtcccggtg cccggccccg gcggcgccgc gaccatcgtc
     1381 aatgcgggaa cgatccgggg cgggaccggc tatgccgtca aggtcgagaa aggcgcgctg
     1441 acgctccgca acagcggcag gatcgtgggc gcaatgctgc tcgccgacgg tgacgacctg
     1501 atcgagaatg aaggcacgtt cgacgtgcgg gcggacagcg acttcggcgc cggcgacgac
     1561 cgcttcgtca atagcggcgt cgtgaagctc ggcacgacct cggcgcccgc cacctaccgc
     1621 ttcctcggcc tcgaacgctt cgacaatcag ggtctggtcg atctccgcaa caccgccgta
     1681 ggcgacgtct tctccctttc gggcgattac gcgggcagcg gcgatgccac ccttgcgctc
     1741 gacgtcgatc ttgccaacaa caaggccgac acgttcgtcg tggaaggcgc ggcgacgggc
     1801 gagacccaga tcgtgctgga cctgaccccc ggcaccgcca gactgaccag cgagagcggg
     1861 atcactttgg tcgaaaccgg agaagactcc aactccgacg ccttcgtcct ggccgaatcc
     1921 tcgcaggaca tcggtttcat ccgctacggc ctcaactatg acgcggcggc gcggcgctac
     1981 gcgctcaccg gcgtcgcgag cgccggcgcc taccgccagc tcaacgcgct cgaaggcgcc
     2041 gaaaatctct ggcacgtctc ggccgacgcc tggcgcgcca atgccgccgc cgagcgcgac
     2101 gcgcgcttcg ccggcgccga cggaccgcgg cgtggaacgc tgtgggcgca ggcgctcggc
     2161 gggcggatct cgcgcgacga ggataccgac gaggccggca tcgccggcgg agaaaccgtc
     2221 gatttcgcct atgagcagag ccggatcggc ggccagctag gcgtcgactt cgccggctac
     2281 gaccgcaacg acagcgggct gcgcatcggc ctcaccgccg gctatatcga ttccgagctg
     2341 gaatcccgcg ccgccgccga gaagctcgac atggagacgt tcaacgtcgg cgcctatgcg
     2401 agcttcacca gcgggcgctt gttcgccggc gcgctcctca agcacgattg gcacgatatc
     2461 ggcttcgaca gcgacgcgga cggcttcgag ggcgacgcgg acggcaggac ctggggcgtc
     2521 gaaggcgaga tcggtgcgcg cttcggcggc ggcggcttct tcgtcgagcc ggtcgccagc
     2581 ctcgcctgga ccagcaccag cctcgacgat ctccaagcgc tcggccagtc gctcgatttt
     2641 cgcggcgccg acggcctgcg cggaaagctc ggagcccgca tcggcgggcg cagcgacgtc
     2701 ggcaacggcg atgccctcgt cttccacgcc gcggccaagg cggtccacga tttcggcggc
     2761 gactatcgcc tcgacctcaa cagcagcggc gccgcccagg agctctcctc gaagcggatc
     2821 ggcacgttcg gcgaaggcct gatcggcgcc tcctatatct ggcgcggcgg gttcgaggcg
     2881 ttcgtcgaag gccaaagcga gctgggcagc gaatatgacg ggctcggcgg ccgcatcggc
     2941 ttccggctga ggctctagtc cagcttgcgc cgataccgga acgtgaggca ccgagggcgc
     3001 cgccgatgtc gcgcgcccgc gccatttccg gtctaaggtg cctcccgtga ccaagcccac
     3061 caccgccgcg cccgacatca ccgcatcgcg cggcctctcg cggtggctca agagccgcca
     3121 caccagcttc gctttctcct cctaccagac cgggcagctg ttcctggtcg gcagccaggc
     3181 cgacggcacc gtgtcggtga accagcatta tttccagcgc gcgatggggc tcgcctggcg
     3241 gccgggccgg ttgtacctcg cctcgcaata tcagctctgg cggctcgaaa acgtgctgcg
     3301 tccgggcgag ctggcgaacc gcgccttcga tctcctgctg gtgccgcgca acgcccagac
     3361 caccggcgac ctcgacatgc acgaaatcgc ggtcgacgat gccggccgcg tgctgttcgt
     3421 caacaccagc tattcctgcc tcgccacgct cgacctcagg aacagcttcc ggccgatctg
     3481 gaagccgcct ttcatctcgc ggctggcggc ggaggatcgc tgccatttga acggcctcgc
     3541 cgtcgggccg cgcggcgagc cgcgttacgt gaccgcggtc agccgctccg atgtcgtgaa
     3601 cggctggcgc gagcggcggg ccgagggcgg cgtgctgatc gacgtcgcga cgggcgggac
     3661 cgtcaccgac caattgtcga tgccgcattc gccccgcctc gtcggagacg acctctattt
     3721 gctcgacagc gggcgcggct tcctcgtccg gatcgacccc aaggacggca gccgcaccga
     3781 catcgccttc tgccccggct tcctccgcgg cctgtcgatc cacgccggcc acgcgctggt
     3841 gaccgtgtcc aagccgcgcg aggcgacgtt cggcgggctc gccctggccg gcgagctcgc
     3901 cgcccg
//
LOCUS       DASVRK010000126         7539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_333592, whole genome shotgun sequence.
ACCESSION   DASVRK010000126 DASVRK010000000
VERSION     DASVRK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333592"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF589_04795"
     CDS             complement(<1..458)
                     /locus_tag="VF589_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256928.1"
                     /translation="MTPLIFAFALAAPLAVAPPPPPPDRFAAFVRASAPLPGLPGATG
                     ELMIAGAAAAVRVRRPGAEDLLDPIGGVEDALSLLADRRVEPLWPAILAWTGPTMERL
                     RDRRLAAARAEVAGGRSMIATSTAASSTRPPVRAAMLLADELWRAGRPAEA"
     gene            682..2985
                     /locus_tag="VF589_04800"
     CDS             682..2985
                     /locus_tag="VF589_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256929.1"
                     /translation="MTIINLASNSYDGTVIIGSSKQRQIGVHLDSAGDFDGDGFDDLM
                     VGAGYDDDEYPRGEVYIFTGSEETLTKEFTFTGPAADYNNYGLGVASIGNFNGDGFDD
                     VAIADRRSGDISVVFGRPDSGEGGGFDIEGVSLFNVDLASAGDVNGDGLDDLLIGLPQ
                     GNSAYVIFGKRDGHADIDLSDLSASEGYRIQGDDDQLIVSVASAGDMNDDGYDDVLIG
                     SPGVFGSYDNGAAYVVFGKEGGFGTVDLETLAPGEGFALEGNGNGSFARTVSSAGDIN
                     GDGFDDIAIAGQGGRIYVVFGGSGGFGGIDLDAMGPGEGFEIKHDFHGVRADGIASAG
                     DINGDGYDDLLIGRPDVNDKRGEAYLIYGRAGGFEGIDLLNLDSDDGFVLRGDDPDDF
                     AGGTVAGAGDLNGDGYDDILIGAPGYNAVPGRQNEVGAVYVIYGGERFAATDFRGGLG
                     NDRAAGGTRADFLSGGAGDDVLSGRGADDYLRGGNGDDVLGGGDQDDRLDGGQGTDTA
                     SYRSATAGVTVDLTLGTQDTSGAGTDTLISIENLIGSDHADNLFGSSVRNLIEGGDGD
                     DYMEGRGGGDRFEGGRGMDTVSYAWASTGVRASLNTTEPQQTNVGLDRFIGVENLRGT
                     AADDVLAGNPDANRLFGGAGDDRLIGNAGGDIMYGGAGRDLFLLRPGDSGLGAPGAAD
                     TIADFSRFEGDRIDLRRIDAVVGTGSNEAFTFIGTASFSGQAGELRYSGNGTQKVVRG
                     DIDGDRIADFAIVVGQVNALAATDFIL"
     gene            3059..4498
                     /locus_tag="VF589_04805"
     CDS             3059..4498
                     /locus_tag="VF589_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8256930.1"
                     /translation="MIDELRLEIGRLRQATAPLEPDADGREALGRQALDHILAYLSQV
                     PDAPSNRPAAEAFARRLAPEFAEQGRDPADIFDYVAACVDRPGFATTSPRFMAYIPGG
                     GLYHSALGDLMAAASNKYSGFASASPGAVRLENATIAWLAEAIGYPPAAAGTLTSGGS
                     IANLTAIVAARDAGDAEGGGIVYATRFAHHCVDKALHIAGRGRAPRRAIATDDRHRMS
                     ADALEEALERDRAAGLRPWLVVASAGTVDTGAVDPLPEIAELCRRHGTWLHVDGAYGG
                     LFALCPEGRDILRGIDVADSVALDPHKTLFLPYGTGAVLVRDGRHLLDSFSASAEYIR
                     PLGEAGVGPSPADLSPELTRHFRALRLWLPLQLAGVAAFRAAQSEKLALARYFHARLG
                     ELDGFEPGPPPDLSVVAFRYQPAEGDADAFNERLLRHVQEEGRVMLSGTRIDGRFTLR
                     CAILSFRTHLEHVDEAIAALVRGVAACGS"
     gene            4605..5570
                     /locus_tag="VF589_04810"
     CDS             4605..5570
                     /locus_tag="VF589_04810"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8256931.1"
                     /translation="MSEAWIWDQYWHSDRVASCFDGRGATNYGDAVASGWRSFFGSLP
                     EGAAILDLCSGNGAVAILAAEAARDFRIVAVDQADIDPAAHVTRFADCLAAIRFMGGV
                     NVEALPFADASFDVAVSQYGIEYSDLGRSIPELWRVLRPAGRARLVLHAAEGEVARWS
                     APHVGDADFLLGEIDLCGKASLCFAAVAAAERDPEVDEGKRRAAEAALAAFADALHQA
                     GRYLPGAADPPMIRHSAATLLDAYRNRGRFERDQLEAVVDSVRSGIEAHRGRVLALLA
                     AAVTAARAEEIAAGLRARGADAASFGLTCPQGLIGHVVELRRPGE"
     gene            complement(5639..>7539)
                     /locus_tag="VF589_04815"
     CDS             complement(5639..>7539)
                     /locus_tag="VF589_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256932.1"
                     /translation="ANRIAGGAGDDRIEGGGGDDLVDGGGGSDTASYASAAGWVRIDL
                     YAAGPQDTLGAGVDTIVRVENVLGSSHGDRIRGERGANLLDGGSGNDRLFGMTGDDVL
                     IGGEGADLLSGLGGLDHMSGGAGDDVYYVEQAGDVTVEAADQGHDIVRAFIDHQLADN
                     IEELRLGGAARAGTGNGLANLIYGTVEGDVLSGLGAADRIEGGDGDDRIDGGADADIM
                     FGGAGNDIYSADEAGDVVAEAAGEGLDEVRTSLTSYTLGGELEALTGTAAAGQALTGN
                     ALANSIAGGAGDDVIDGGAGDDRLDGGIGSDSVSYASASAGVKLNFFLAGPQDTVGAG
                     IDTLARFENAIGSSFDDVLGGNGLANRLEGGAGGDRLNGMAGNDLLIGGEGGDVLTGN
                     GGDDMMQGGAGDDIFYVEQAGDRVVEDADQGRDVVQAYVDHALADNVEELRLAGSARS
                     GTGNGGANRIVGTGFGDTLSGLGGGDLLEGGNAADTLSGGADKDRLLGGAGDDLLTGD
                     DADDQLWGQQGLDTMTGGAGADLFVFDDGQTGASEAAADVVTDFAAGDRFHLRAIDAD
                     AAAAGDQAFRFLGNGAFTGGGVGGELRYEQVGGSTFIQGDVDGDGIADLFIRLDGRHD
                     LAAADFVL"
BASE COUNT         1054 a         2717 c         2644 g         1124 t
ORIGIN      
        1 gcctccgccg gccggccggc gcgccacagc tcgtcggcca gcagcatcgc cgcgcggacc
       61 ggcgggcgcg tgctcgaggc ggcggtcgaa gtcgcgatca tgctccgccc gccggcgacc
      121 tcggcccgcg ccgcggccag ccggcgatcg cgcagccgct ccatcgtcgg gcccgtccag
      181 gccaggatcg cgggccatag cggctccacc cgccggtcgg cgagcagcga gagcgcatcc
      241 tcgacgccgc cgatcgggtc gaggagatcc tccgccccgg gccgcctcac ccgcacggcg
      301 gcggcggcgc cggcgatcat cagctcgccg gtcgcccccg gcagccccgg cagcggcgcg
      361 ctcgcccgca cgaaggcggc gaaacgatcg ggcggcggcg gcggcggggc gaccgcgagc
      421 ggcgccgcaa gcgcgaaggc gaagatcaac ggcgtcattg cccggggatt tcgcacaaac
      481 gccgccgagc ggcaacgggc cggtgcccgg gcggttgcgg ccgcggcgga agaccggccg
      541 aggggggcgc cagcgctgtc ccggcccggg agagccgcgc gcatcgtcgc agcttcctgc
      601 gccaagtgcc tctgatttct cggctgccct cctctaccat gctatgctat ggcgggcgct
      661 tgatgcgtgg ggagaatgcg cgtgacgatc atcaatctgg catcgaacag ctatgacggc
      721 accgtcatca tcggcagcag caagcagcgc cagatcggag tgcacctcga ctcggcgggc
      781 gatttcgacg gcgacggctt cgacgacctg atggtcgggg ccggctacga cgacgacgaa
      841 tatccccgcg gtgaagtcta tatcttcacc ggcagcgagg aaacgctgac caaggagttt
      901 actttcaccg ggcccgcggc cgactacaac aattacggct taggagtcgc ctcgatcggc
      961 aacttcaacg gcgacggctt cgacgacgtc gcgatcgccg accggcgttc gggcgacatc
     1021 agcgtcgtgt tcggccgtcc cgatagcggc gagggcggcg gcttcgacat cgagggggtc
     1081 tctctcttca acgtggacct cgcctccgcg ggcgacgtca atggcgacgg gctggacgat
     1141 ctgctgatcg gcctccccca ggggaacagc gcctacgtga tattcggcaa gcgggatggc
     1201 cacgccgata tcgacctttc cgacctgtcc gccagcgagg gctaccggat ccagggcgac
     1261 gacgatcaat tgatcgtcag cgtcgcttcg gccggagaca tgaacgacga cggctatgac
     1321 gacgtactga tcgggtcccc tggggtgttc ggcagctacg ataacggggc tgcctatgtc
     1381 gtgttcggca aggagggcgg cttcggcacc gtcgacctcg agactctcgc gcctggcgaa
     1441 ggcttcgccc tggagggcaa tggcaacggc tctttcgccc gcaccgtttc ctcggccggc
     1501 gatatcaacg gcgacggctt cgacgacatc gcgatcgccg ggcagggcgg ccgcatctat
     1561 gtggtgttcg gcgggagcgg cggcttcggc ggcatcgatc tcgacgccat ggggcccggc
     1621 gaggggttcg agatcaaaca tgatttccac ggggtgcgcg ccgacgggat agccagcgcc
     1681 ggcgacatta acggcgacgg ctatgacgat ctgctgatcg gcaggcccga cgtcaacgac
     1741 aaacgcggcg aggcctatct gatctacggc agggccggcg ggttcgaagg catcgacctg
     1801 ctcaatctcg attccgacga cggcttcgtg ctccggggcg acgacccgga cgattttgcc
     1861 ggcgggaccg tggcgggggc cggcgatctc aacggcgacg gctatgacga tatcttgatc
     1921 ggagcgccgg gctataacgc cgtaccgggc cggcaaaacg aggtcggcgc cgtctatgtg
     1981 atctatggcg gcgagcgctt cgccgcgacc gacttccgcg gcgggctcgg caacgaccgg
     2041 gcggccggcg gcacccgcgc ggacttcctc agcggcggcg ccggcgacga cgtgctgtcg
     2101 ggacgcggcg cggacgatta tctgcgcggc ggcaatggcg acgatgtgct cggcggcggc
     2161 gatcaggacg accggctcga cggcggccag ggcaccgaca ccgcctccta ccgcagcgcg
     2221 accgcgggcg tcacggtcga cctcaccctc ggcacccagg acacaagcgg ggccggaacc
     2281 gacaccttga tctcgatcga gaatctgatc gggtcggacc atgccgacaa tttgttcgga
     2341 tcctcggtcc gcaacctgat cgagggcggc gacggcgacg actacatgga gggccgcggc
     2401 ggcggcgacc ggttcgaggg cgggcgcggc atggatacgg tgagctatgc ctgggcctcg
     2461 acgggcgtgc gggcgtcgct caacacgacc gagccgcagc agaccaatgt cgggttggac
     2521 cgcttcatcg gcgtggagaa tttgcgggga acggcggcgg acgacgtgct cgccggcaat
     2581 ccggacgcga accggctgtt cggcggcgcc ggcgacgatc gcctgatcgg caatgccggc
     2641 ggagacataa tgtacggcgg cgccggccgc gaccttttcc tgctccgccc cggcgacagc
     2701 ggcctcggcg cgccgggcgc cgccgacacc atcgccgact tcagccgctt cgagggcgac
     2761 aggatcgatt tgcggaggat agacgcggtc gtcggaacgg gctcgaacga agccttcacc
     2821 ttcatcggca ccgcctcctt ttcgggccaa gcgggcgagc tgcgctactc cggcaacggc
     2881 acccagaagg tggtgagggg cgacatcgac ggcgacagga tcgccgactt cgccatcgtg
     2941 gttgggcagg tcaacgcttt ggccgcgacc gacttcatcc tctgacggga ggcggccctg
     3001 ctcgcgccgg gcgagcggcc gcttccgctt cggcgcctga tgtgacatca gcccggcgat
     3061 gatcgacgaa ctgaggcttg agatcgggcg gctgcggcag gcgacggcgc cgctcgagcc
     3121 ggacgcggac gggcgcgagg cgctggggcg gcaggcgctg gaccatatcc tcgcctatct
     3181 cagccaggtt cccgacgccc cgagcaaccg ccccgcggcg gaagccttcg cccgccgcct
     3241 cgcgcccgaa ttcgccgagc aggggcgcga ccccgccgac atcttcgatt atgtcgcggc
     3301 ctgcgtcgac cggccgggct tcgccaccac ctcgccgcgc ttcatggcct acataccggg
     3361 cggcggcctt tatcattcgg cgctcggcga cctgatggcg gcggcctcca acaaatattc
     3421 gggcttcgcc tcggccagcc ccggcgccgt gcggctggaa aatgcgacca ttgcctggct
     3481 ggccgaggcg atcggctatc cgcccgccgc cgcgggcact ttgacctcgg gcgggagcat
     3541 cgccaacctc accgccatcg tcgccgcccg cgatgccggc gacgccgagg gcggcggcat
     3601 cgtctatgcg acccgcttcg cccatcattg cgtcgacaag gcgctccaca tcgccgggcg
     3661 cgggcgtgcc ccgcggcggg cgatcgccac cgatgaccgc catcgcatgt cggcggacgc
     3721 gctcgaggaa gcgctcgagc gcgaccgcgc cgccggcctg cgcccctggc tggtggtggc
     3781 ctcggcgggg acggtcgaca ccggcgcggt cgatccgctg cccgagatcg cggagctctg
     3841 ccgccgccac ggcacctggc tccatgtcga cggcgcctat ggcggcctgt tcgccctgtg
     3901 ccccgaaggg cgcgacatat tgcgcggcat cgacgtcgcc gacagcgtcg cgctcgatcc
     3961 gcacaagact ttgttcctcc cttacgggac cggcgcggtg ctggtgcgcg acggccgcca
     4021 ccttctggat tcgttcagcg ccagcgccga atatatccgc ccgcttggcg aggccggggt
     4081 cggcccctcg cccgccgatc tgtcgcccga gctgacccgc catttccggg ccttgcgcct
     4141 gtggctgccg ctgcagctcg ccggcgtcgc cgccttccgc gccgcccagt ccgagaagct
     4201 ggcgctggcc cgctatttcc acgcccgcct cggcgagctc gacgggttcg agcccggccc
     4261 gccgcccgat ttgtcggtgg tcgcgttccg ctaccaaccg gcggaaggcg atgccgacgc
     4321 gttcaacgag cggctgctgc gccatgtcca ggaggaaggg cgggtgatgc tgagcggcac
     4381 ccgcatcgac ggccgcttca ccctgcgctg cgccatcctc agcttccgca cccatttgga
     4441 gcatgtcgac gaggcgatcg ccgccctcgt ccgcggcgtc gccgcctgcg ggtcctgacg
     4501 tctttcccgc cgcgctcccg cgagaaaagg tcgtccccgc cgaaaaagct ggcttgtccg
     4561 gaacttgccc ataagatgtg tccgattccg tacgggggca tccgatgtcg gaagcgtgga
     4621 tctgggacca atattggcat tcggaccggg tcgcttcctg cttcgacggg cgcggcgcga
     4681 ccaattacgg cgacgcggtg gcgtccggct ggcgcagctt cttcggctcg ctgcccgagg
     4741 gcgccgcgat cctcgacctg tgctcgggca acggcgccgt cgccatcctt gccgcggagg
     4801 ccgcgcgcga tttccggatc gtcgcggtcg accaggccga tatcgatccc gccgcccatg
     4861 tcacccgctt cgccgattgc ctggccgcga tccgcttcat gggcggcgtc aatgtcgagg
     4921 ctttgccgtt cgccgacgcc agcttcgacg tcgcggtcag ccaatatggt atcgaatata
     4981 gcgacctcgg ccgctccatc ccggagctgt ggcgggtgct gcggccggcg gggcgcgcgc
     5041 ggctggtgct tcatgccgcc gaaggcgagg ttgcgcgctg gtcggcgccg catgtcggcg
     5101 acgctgattt cctgctcggc gagatcgacc tgtgcggcaa ggccagcctt tgcttcgccg
     5161 ccgtcgccgc cgccgagcgc gacccggagg tggacgaagg caagcgccgc gcggccgaag
     5221 ccgccctggc ggcgttcgcc gacgcgctcc atcaggcggg gcgctacctg cccggcgccg
     5281 ccgacccgcc gatgatccgc cattcggccg cgaccctgct cgacgcctat cgcaatcgcg
     5341 gccggttcga gcgcgaccag ctcgaagcgg tggtggacag cgtccgctcc ggcatcgagg
     5401 cccatcgcgg gcgggtgctg gcgctgctcg cagccgcggt caccgccgcg cgggccgagg
     5461 agatcgccgc cggcctgcgc gcgcgcggcg cggacgcggc gagcttcggc ctcacctgcc
     5521 cgcaaggcct gatcggccac gtcgtcgagc tccggcgccc gggcgagtga ggctaacgat
     5581 cagggacgag cgggcgggcg atcatgccgg ctgagcggcg cgatcctccc gtccggcctc
     5641 agaggacgaa gtcggccgcc gccaggtcgt ggcggccgtc gaggcggatg aacaggtcgg
     5701 caatgccgtc gccgtccacg tcgccctgga tgaaggtgct gccccccacc tgctcgtagc
     5761 gcagctcgcc gccgacgccg ccgcccgtga aagcgccgtt gccgaggaag cggaaggcct
     5821 ggtcgccggc cgcggcggca tcggcgtcga tcgcgcgcag gtggaaccgg tcgccggcgg
     5881 cgaagtcggt gacgacgtcg gccgcggctt cgctggcgcc ggtctggccg tcgtcgaaca
     5941 cgaacaggtc cgcgcccgcc ccgccggtca tcgtgtcgag cccctgctgg ccccagagct
     6001 ggtcgtcggc atcgtcgccg gtcagcagat cgtcgccggc gccgccgagc agccggtcct
     6061 tgtcagcgcc gccggacagg gtgtcggccg cgttgccgcc ttcgagcagg tcgccgccgc
     6121 caaggccgct cagcgtgtcg ccgaagccgg tgccgacgat ccggttggcg ccgccattgc
     6181 cggtgccgct acgagccgag ccggcgaggc gcagctcctc gacattgtcg gcgagcgcgt
     6241 gatcgacata ggcctgcacc acgtcgcggc cctggtcggc atcctcgacg acgcggtcgc
     6301 ccgcctgctc gacgtagaag atgtcgtcgc cggcgccgcc ctgcatcatg tcgtcgccgc
     6361 cattgccggt cagcacgtcg ccgccctcgc cgccgatcag caggtcgttc ccggccatgc
     6421 cgttgaggcg gtcgccgccg gcgccgccct cgagccggtt ggcaaggccg ttgccgccga
     6481 gcacgtcgtc gaacgacgat ccgatcgcat tttcgaagcg ggcgagggtg tcgatccccg
     6541 cgcccaccgt atcctgcggc ccggcgagga agaagttgag cttcaccccg gccgaggccg
     6601 aagcgtagct caccgagtcg ctgccgatgc cgccgtcaag ccggtcgtcg ccggcgccgc
     6661 cgtcgatcac gtcgtcgccc gcgccccccg cgatgctgtt ggcgagcgca ttgccggtca
     6721 gcgcctgccc ggcggcggcg gtgccggtca gcgcctccag ctccccgccg agcgtgtagg
     6781 aagtgagcga ggtgcggacc tcgtcgagac cctcacccgc cgcttcggcc accacgtcgc
     6841 cggcctcgtc ggccgaataa atgtcattgc ccgccccgcc gaacatgatg tcggcatcgg
     6901 cgccgccgtc gatccggtcg tcgccgtcgc cgccctcgat ccggtcggcc gcgccgaggc
     6961 cgctcaggac atcgccttcg acggtcccgt agatgaggtt ggcgaggccg ttgccggtgc
     7021 cggcccgggc ggcgccgccg agccgaagct cctcgatatt gtcggcaagc tggtggtcga
     7081 tgaaggcgcg gacgatgtca tggccctggt cggccgcctc gacggtgacg tcgccggcct
     7141 gctcgacata atagacgtcg tcgcccgcgc cgcccgacat atggtcgagg ccgccgaggc
     7201 cgctcaggag gtcggcgcct tcgcccccga tcaggacgtc gtcgccggtc atgccgaaca
     7261 gccggtcgtt gccgctgccg ccgtcgagca gattggcgcc gcgctcgccg cggatccggt
     7321 cgccatggct cgatccgagg acattctcga cgcggacgat cgtgtcgacc ccggcgccga
     7381 gcgtgtcctg cggcccggcg gcatagaggt cgatccggac ccagccggcg gccgatgcat
     7441 agctcgccgt gtcgctgccg ccgccgccgt cgacaaggtc gtcgccgccg ccgccctcga
     7501 tcctgtcgtc gcccgcgccg ccggcgatgc ggttggcga
//
LOCUS       DASVRK010000127         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_271545, whole genome shotgun sequence.
ACCESSION   DASVRK010000127 DASVRK010000000
VERSION     DASVRK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271545"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1585)
                     /locus_tag="VF589_04820"
     CDS             complement(<1..>1585)
                     /locus_tag="VF589_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256933.1"
                     /translation="DYDRVWSSVSHTLAAYVERLDLTGAADLAGTGNSLANTIFGNDG
                     ANILSGGGDNDYLAGGIGDDSMSGGVGDDIYIVDDVLDGAFESASEGSDEVISSVSFT
                     LGDHIEKLTLAFTAEQGTGNALANVIVGNNQANRIDGGAGADTMDGGFGNDLYIIDES
                     GDLVSETSGAGGIDEVQSSVNHALGANFENLTLTGAATLATGNALANILVGNFQNNRL
                     DGAVGADTMEGGGGDDVYVVDDGGDVASETGGSGTDKVETSVSVVLADNIENLTLFGS
                     AAIAGTGNMLANTIVGNAAANAIGGEDGADTLEGGGGNDTLTGGLGADTMLGGLGNDI
                     FEVDDAGDVATESAGGGIDRVRSSVSFALDANIERLELTGAGAINGTGNGGVNFLFGN
                     VSNNILNGAGGADHMEGGAGDDIYVVDGVNEAVVETAGEGTDTVETNIGYTLGAHLEN
                     LTLHGPGDIDGTGNALDNRLVGNSGANRLDGLGGADYMEGGDGDDVYVVRQTGEFPNE
                     TSSTGGTDTVATFVSTGLGA"
BASE COUNT          216 a          532 c          526 g          311 t
ORIGIN      
        1 tggcgccgag gccggtgctg acgaaggtcg cgaccgtgtc ggtgccgccg gtgctgctgg
       61 tctcgttcgg gaattcgccg gtctggcgca ccacatagac gtcgtcgccg tcgccgcctt
      121 ccatatagtc ggcgccgccg agcccgtcga ggcggttggc gccgctattg ccgaccagcc
      181 ggttgtcgag cgcgttgccg gtgccgtcga tgtcgcctgg cccgtggagc gtcagattct
      241 cgagatgggc gccgagcgtg tagccgatat tggtctcgac cgtgtcggtg ccttccccgg
      301 ccgtttcgac gacggcttcg ttgacgccat cgacgacgta aatatcgtcg ccggcgccgc
      361 cctccatatg gtccgcgccg cccgccccgt tgaggatgtt gttcgagacg tttccgaaca
      421 ggaagttgac gccgccattg ccggtgccgt tgatcgcccc cgccccggtc agctcgagcc
      481 gttcgatatt ggcgtcgagc gcgaagctca ccgagctgcg gacgcggtcg atgcccccgc
      541 cggcgctttc ggtggcgaca tcgccggcgt catcgacttc gaagatatcg ttgccgagcc
      601 cgccgagcat cgtgtccgcg ccgagcccgc cggtgagggt atcgttgccg ccgccgccct
      661 ccagcgtgtc ggcgccgtcc tcgccgccaa tggcgttggc ggcggcgttg ccgacgatgg
      721 tgttggcgag catgttgccg gtcccggcga tcgcggccga gccgaacagg gtcagatttt
      781 cgatattgtc ggccaggacg acgctgaccg aagtctcgac cttgtcggtg ccgctgccgc
      841 cggtctcgct cgcgacgtcg cccccatcgt cgacaacata gacgtcgtcg ccgccgccgc
      901 cctccatcgt gtcggcgccg acggcgccgt cgagccggtt gttctggaaa ttgccgacga
      961 ggatgttggc gagcgcgttg ccggtcgcca gcgtggcggc gccggtcagc gtcagattct
     1021 cgaaattcgc gcccagcgcg tggttgaccg agctttggac ttcgtcgatc ccgccggctc
     1081 cggaggtttc ggacaccaag tcgcccgatt catcgatgat gtagaggtcg ttgccgaagc
     1141 cgccgtccat cgtgtccgca ccggcgccgc cgtcgatccg gttggcctga ttgttgccga
     1201 cgatgacgtt cgccagcgca tttcccgtcc cttgctcggc ggtaaaggcg agcgtcagtt
     1261 tctcgatgtg gtcgccgaga gtgaagctga cggagctgat gacctcgtcg gagccctccg
     1321 atgcgctttc gaaggcgccg tccaagacat cgtcgacgat gtagatgtcg tcgccgacgc
     1381 cgcccgacat cgaatcgtcc cctatcccgc ccgcgagata gtcgttgtcg cccccgccgg
     1441 agaggatgtt cgcgccgtca ttgccgaaga tggtgttggc gagactgttg ccggtccccg
     1501 cgaggtcggc cgcgccggtg aggtcgagcc gctcgacata cgccgcgagc gtgtggctta
     1561 ccgaactcca gacacgatca tagtc
//
LOCUS       DASVRK010000128         4446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_271571, whole genome shotgun sequence.
ACCESSION   DASVRK010000128 DASVRK010000000
VERSION     DASVRK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271571"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VF589_04825"
     CDS             complement(<1..787)
                     /locus_tag="VF589_04825"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX8256934.1"
                     /translation="MPFLSPQRLAALSPRERLFLAAGLAALLAFLIYLLMPAAEEPPV
                     ELGEEAAMPAPEFAPAPPPAASAPLPPAAPPASVAGLVLHGVLGGGPGGGAAIIRFPD
                     GSQRAVRVGREFLPGLTLKEVGLRHALIATAGGDMRLEFNKPAEAVAGTASAPAAPSP
                     AEASAEQRRRRETLQYRMGLAPRTEGGRVVGYAMRPGASLPLFEQAGLRPGDVIVRVN
                     GKALKSSEKVLELSDELATSYTAEIEYIRGGRRMKGSVPVNKKP"
     gene            complement(815..1606)
                     /locus_tag="VF589_04830"
     CDS             complement(815..1606)
                     /locus_tag="VF589_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8256935.1"
                     /translation="MPPAELLGQPAWAWAGGGALLGLVAGSFIAAILIRWPKGESVLR
                     GRSRCDLCGTTLGAAELVPVFSWLALRGRCRHCGGRIDARHAGVEVTAAIVGFAAFHV
                     FAPAAALVAAVFGWWLLLLAALDIEHHWLPDRLTLPLIPAGLAAAWLGLGAPLADRLI
                     GAAAGYALLAGIAIVYRRLRGREGLGGGDPKLLAALGAWLGWQQLPFVLLGAGLLGLN
                     AILLIQLRGRRLAATDRLPLGTLMALVAWPIWLLASGHQAVTLAS"
     gene            complement(1800..2195)
                     /locus_tag="VF589_04835"
     CDS             complement(1800..2195)
                     /locus_tag="VF589_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8256936.1"
                     /translation="MKVLAFSALGVLALAGCAGSQSSTLVEQGYQPGALGVAAIDRGD
                     WAAAEKGLMEKRGISANDPARLINLGKVYMETGRRGEALSAWRLALASDRHREVETID
                     GRVVSTEDLARQALAMYETDVRSASADPR"
     gene            2422..>4446
                     /locus_tag="VF589_04840"
     CDS             2422..>4446
                     /locus_tag="VF589_04840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8256937.1"
                     /translation="MEYRLCLALLLSAAAPPALAAPPAGPTPIFTPADLFSLEVASDP
                     QISPDGSRIAYVRRSGDIMTDRMRPSIWIIDTRTGRQMPLAAGPGSHSQPRWSPDGRR
                     LAYISTAEGDAAQLFVRWMDGGESARITGLPDAPSSIAWSPDGRQIAYAMFVPDEGLK
                     LGSTPKKPEGAKWAEPLEIHSAVTYRTDEAGYAKPGYSHVFLVSADGGAPRQLSFGAV
                     NDNGPLSWSPDGGTIYFSGNRDPDWERQPVDTEVYALNVAGGGITALTDREGPDNGPV
                     VSPDGRWIAYTGFDDQRLGYHNTILYVMERGGGGRRELTRSLDRSVGSPLWSADSRSI
                     YVDYDDEGVTKVARVSLDGSVRTVAEGLSGSGFDRPYTGGSFTVARDGTLAISSGTAT
                     RPADVALVRGGAKRQLTRLNAELLDAKRLGEVRKIQARSFDNRTVDAWLTLPPSYSEG
                     QRVPLILEIHGGPFAAYGPHFATDNQLYAAAGYAVLSVNPRGSTSYGADFANQIHHSY
                     PGRDYDDLMVAVDLAIAQGIADPDRLFVTGGSGGGVLTAWIVGKTNRFKAAATQKPVI
                     DWASFALTADQPAFFARYWFGKFPWEDPQGYWARSPLSLVGNVKTPTLVVVGSEDYRT
                     PVSEAEQYYSALQLLGVPTALVKVPGASHGTIAGRPSHSGAKAGAILAWFE"
BASE COUNT          700 a         1616 c         1514 g          616 t
ORIGIN      
        1 atggtttctt gttgactggg acgctgccct tcattctgcg gccgccacga atatactcga
       61 tctcggcggt gtaggaggtg gcgagctcgt ccgaaagctc gagcaccttc tcgctgctct
      121 tcaaggcctt gccgttgacg cggacgatca cgtcgcccgg tcgcagcccc gcttgctcga
      181 acaagggcag gctggccccg ggccgcatgg cgtagccgac cacgcggccg ccttcggtcc
      241 gcggcgccag gcccatccga tattgcagcg tctcgcggcg acggcgctgc tcggcggagg
      301 cttcggccgg tgacggcgcc gccggcgcgc tggccgtgcc cgccaccgct tcggcgggct
      361 tgttgaattc gagccgcatg tcgccgcccg cagtcgcgat cagcgcatgg cgcagcccca
      421 cctccttgag cgtcaggccc ggcaggaact cgcgcccgac gcgcaccgcc cgctgactgc
      481 cgtccggaaa acggatgatg gccgcgccgc cgcccggccc gccgccgagc acgccgtgaa
      541 ggaccagccc cgccaccgac gcgggcggag ccgccggcgg tagcggcgcg ctcgccgcgg
      601 gcggcggcgc cggcgcgaat tcgggcgccg gcatggcggc ttcctcgccc aactcgaccg
      661 ggggctcctc ggcggccggc atgagcagat agatgaggaa agcgagcaga gcggcgaggc
      721 ccgccgcgag gaacaggcgt tcgcgggggc tgagggcggc gagccgttgg ggcgatagga
      781 aaggcatcgg aatcgaggcg gccatccttg ggggctatga cgccagtgtg acagcctggt
      841 gaccgctggc aagcagccag atcggccacg ccaccagcgc catcagggtg ccgagcggaa
      901 gccggtcggt ggctgcgagg cgccgcccgc gcagctggat cagcaggatc gcgttgaggc
      961 cgagcagccc ggcgccgagc agcacgaacg gcaattgctg ccacccgagc caggcgccga
     1021 gcgcggccag cagcttggga tcgccgccgc cgagcccctc ccgcccccgc agccgccgat
     1081 agacgatcgc gatcccggcg agcagcgcat agccggcggc cgcgccgatc agccggtcgg
     1141 cgagcggggc cccgagcccg agccacgccg ccgccagccc ggccgggatc agcggcagcg
     1201 tcagccggtc cggcagccaa tgatgctcga tgtcgagcgc ggccaggagc agcagccacc
     1261 agccgaagac cgccgccacg agcgccgcgg cgggcgcgaa gacgtgaaag gcggcgaagc
     1321 cgacgatcgc ggccgtgacc tcgaccccgg catggcgcgc gtcgatccgg ccgccgcaat
     1381 ggcggcagcg gccgcgcagc gcgagccagg agaagaccgg caccagctcc gccgccccga
     1441 gcgtggtgcc gcaaaggtcg cagcgcgagc ggccccgcag caccgattcg cccttcggcc
     1501 agcggatcag gatggcggcg atgaagcttc cggcgacgag gccgagcagc gcccccccgc
     1561 cggcccaggc ccaggcgggc tggccaagca gctcagctgg cggcatccgc gtcgcacctc
     1621 tccccgctcg gcagacgcgc cgcggcggcg ggacgcctct tcatccttca caaacgcttg
     1681 cagcccacgc gcagccttcg tcattccggc gaaagccgga atctcccgcc gtgaaggaag
     1741 agagattcca gcttacgctg gaatgacgat ggcgacacta agccgcgggc ggcgccggtt
     1801 caacgggggt cggcggaggc gctgcggacg tcggtctcgt acatggccag cgcctggcgg
     1861 gcgagatctt ccgtcgagac cacccggccg tcgatggttt cgacctcgcg gtgccggtcc
     1921 gacgcgagcg ccagccgcca ggcggacagc gcctcgccgc ggcggccggt ttccatatag
     1981 accttgccga ggttgatcag ccgggcggga tcgttggcgg agatgccgcg cttctccatc
     2041 agcccttttt ccgccgccgc ccaatcgccg cggtcgatcg cggcgacgcc gagcgcgccg
     2101 ggctggtagc cttgttcgac cagggtggag gattggctgc cggcgcagcc ggcgagagcg
     2161 aggacgccga gcgcggaaaa agcgagcacc ttcatcgtga gtctcccacg aggccgaata
     2221 ttatagtccg caaagttaca gaaatatgac gaatgtgcaa caaagaagtg acacccgcgc
     2281 cggcgccttg ccgcgacggc cggagccggc gattgcgccc gattttccgc tgtccttcgc
     2341 gccgtttcca gctgaggaag cgggttgcgc gccacgcgac ccggcttcta agcttccccc
     2401 aaaacctcag gggacgcgct catggaatat cgcctttgcc tcgctctgct gctctccgcc
     2461 gccgctcccc cggcgctcgc cgcgccgccc gccggaccga cgccgatctt cacccccgcc
     2521 gacctgttca gcctcgaggt cgcgtccgac ccgcagatca gccccgacgg cagccgcatc
     2581 gcctatgtcc gccgttcggg cgacatcatg accgaccgga tgcggcccag catctggatc
     2641 atcgacacca ggaccggccg gcagatgccg ctcgccgccg gccccggctc gcacagccag
     2701 ccgcgctggt cgcccgacgg ccgccgcctc gcctacatct cgaccgccga gggcgatgcc
     2761 gcgcaattgt tcgtccgctg gatggacggc ggcgaatcgg cgcggatcac cggccttccc
     2821 gacgcccctt ccagcatcgc ctggtcgccc gacggccggc agatcgccta tgcgatgttc
     2881 gtgcccgacg agggcctgaa gctcggctcg acgccgaaga agcccgaagg cgccaaatgg
     2941 gccgagccgc tcgagatcca ctcggcggtc acctaccgga ccgacgaagc cggctatgcg
     3001 aagcccggct acagccacgt cttcctggtg tcggccgacg gcggcgcacc ccgccagctg
     3061 agcttcggcg cggtcaacga caacggaccg ctctcctggt ctccggacgg cggcaccatt
     3121 tatttcagcg gcaaccgcga tcccgattgg gagcgccagc cggtcgatac ggaggtctat
     3181 gcgctgaacg tcgccggcgg cggcataacc gcgctcaccg atcgcgaggg gccggacaac
     3241 ggcccggtcg tctcgccgga cgggcgctgg atcgcctata ccggcttcga cgaccagcgg
     3301 ctcggctatc acaacaccat cctctatgtg atggagcggg gcggcggcgg acggcgcgag
     3361 ctcacccgct cgctcgaccg cagcgtcggc tccccgctct ggtccgccga cagccgctca
     3421 atctatgtcg attatgacga tgagggcgtc accaaggtcg cccgtgtatc gctcgacggt
     3481 tcggtgcgga cggtggcgga ggggctcagc ggctccggct tcgaccggcc ttataccggc
     3541 ggctccttca ccgtcgcccg cgacgggacg ctggcgatca gcagcggcac ggccacgcgg
     3601 cccgccgacg tcgcgctggt ccgcggcggc gccaagcgcc agctcacccg gctcaacgcc
     3661 gaattgctcg acgccaagcg gctcggcgaa gtgcgcaaga tccaggcgcg gtcgttcgac
     3721 aaccgcaccg tcgatgcctg gctgacactg ccgccgagct atagcgaggg ccagcgggtg
     3781 cctttgatcc tcgaaatcca tggcgggccg ttcgccgcct acgggccgca cttcgcgacc
     3841 gacaaccagc tctacgccgc cgccggctat gcggtgctgt cggtcaatcc gcgcggatcg
     3901 accagctacg gcgccgattt cgccaaccag atccaccata gctatcccgg ccgcgactat
     3961 gacgatctga tggtcgcggt cgacctcgcc atcgcgcagg ggatcgccga tccggaccgc
     4021 ctgttcgtca ccggcggctc gggcggcggc gtgctcaccg cctggatcgt cggcaagacc
     4081 aaccgcttca aggccgccgc gacgcaaaag ccggtgatcg actgggcgag cttcgcgctc
     4141 accgcggacc agccggcctt cttcgcgcgc tactggttcg gcaagttccc gtgggaagat
     4201 ccacagggct attgggcgcg ttcgccgctg tcgctggtgg ggaacgtgaa gacgccgacc
     4261 ctggtcgtcg ttggaagcga ggattaccgc acgccggtca gcgaggcgga gcaatattat
     4321 tcggcactgc agcttttagg agtgccgacg gcgctcgtga aggtgccggg ggcaagccat
     4381 ggtaccatcg ctgggcgccc ttcgcacagc ggcgccaagg caggcgccat tctggcctgg
     4441 ttcgaa
//
LOCUS       DASVRK010000129         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_271976, whole genome shotgun sequence.
ACCESSION   DASVRK010000129 DASVRK010000000
VERSION     DASVRK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271976"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VF589_04845"
     CDS             complement(<1..635)
                     /locus_tag="VF589_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256938.1"
                     /translation="MEQNQNSFTHRAATDEPASPVNPRTARAPGAPGAHAAPPDPPEF
                     AEPPLLSDALTEAMSETLAETFPPGGRKPRHDGWTPEAINIFLRELSATGVVEQAARA
                     AGLSASAAYSFRNRRQGRAFAKMWDAVLVHRARARVAGELQGGAIAGCVSIRKRDGVV
                     VGEYHYYDNRLAMALLTRLDRLAEKEAASEAQLRALSEDLDDYLDCVAAGG"
     regulatory      1176..1267
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1445..2593
                     /gene="gcvT"
                     /locus_tag="VF589_04850"
     CDS             1445..2593
                     /gene="gcvT"
                     /locus_tag="VF589_04850"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX8256939.1"
                     /translation="MSDPMSDNPEQPLPLDGWHRARGARMVPFAGYAMPIQYDGILAE
                     HRWTRQSAGLFDVSHMGQMILSGAGMDGGMDAGLDAALESVLPADLSLLKDGRLRYSL
                     LLGDEGGIIDDLMVTRRGDHFYMVVNGATKHGDIEHLRARLPESVRLEHLEAQALLAL
                     QGPKAVEVLARLAPGVDALTFMTGGSFELEGVPAWISRSGYTGEDGFEISIPADAAER
                     VAERLAAEHEVRPIGLGARDSLRLEAGLPLYGHDLDAATTPIEADLGFAISKRRRLEG
                     GFPGAARIQHELEHGAIRRRVGLAVLGRQPVREGAMVVDGEGNEVGTVTSGGFSPMLE
                     APIAMAYVPAAMAEAGAVRLAARGKIFEAKVVPMPFVPHRYHRKGAAR"
     gene            2590..2961
                     /gene="gcvH"
                     /locus_tag="VF589_04855"
     CDS             2590..2961
                     /gene="gcvH"
                     /locus_tag="VF589_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX8256940.1"
                     /translation="MTRYFTKEHEWISVEGDRATVGITDFAQGQLGDVVFVEVPAAGA
                     QLKKGGEAAVVESVKAASDVFAPVSGEVVEGNQALVDDPALVNSDAEGEGWFFKLILS
                     DNHELSGLMSQDEYKAFAESQ"
BASE COUNT          484 a          953 c         1123 g          493 t
ORIGIN      
        1 tcgccgccgg cggcgacgca gtcgagatag tcgtcgagat cctcggacaa agcgcgcagc
       61 tgcgcctcgc tcgccgcctc tttctcggcg agccggtcga gccgggtgag caacgccatg
      121 gcgagccggt tgtcgtaata atgatattcg ccgacgacga cgccgtcgcg cttgcggatg
      181 ctgacgcagc cggcgatggc gccgccctgc agctcgccgg cgacgcgcgc gcgggcgcgg
      241 tggacgagca ccgcgtccca catcttggcg aaggcgcggc cttggcggcg gttgcggaac
      301 gaataggcgg cggaggcgga gaggccggcc gcgcgcgccg cctgctcgac caccccggtg
      361 gccgaaagct cgcgcaggaa gatgttgatc gcctccggcg tccagccgtc gtggcgcggc
      421 ttgcgcccgc cgggcgggaa ggtttcggcg agggtctcgg acatagcttc ggtgagcgcg
      481 tcggagagga gcgggggttc ggcgaattca gggggatcgg gcggcgcggc gtgggcgccc
      541 ggcgcgccgg gcgcccgggc ggtgcgcggg ttgacgggag aggccggctc atcggtcgcg
      601 gccctgtggg tgaaagagtt ctggttctgt tccatccggc agggtaagcc acaaagcggt
      661 gtctgtagga aagaaccttt ttgcggaggc aaagtgacat ctgcgaacgc agtgtgtcac
      721 ggccaacttt gtgcccgaca gcttcggccg ctgcctcgag atgatcataa tcaaagtcgc
      781 cgagcagatg gacgtacttc atcatcctct gccaatgacc ggaaaggcgc cgcgtcctgc
      841 gcttcggttc atcgtgtggc tcgagttgat ttgcccccaa tagttcggca gcctcgagca
      901 tcccctggat ccgagcttcg acgacgctaa cctgttggag cgcttcgagc atgccgcgct
      961 caaagttaag agctgtgtcg cgatgccgcg ccgcctcgag ccggtaaggc caagaccggt
     1021 cgggggaact gtggtgccca cgcttcgccg cccagaatca gcggctgccg aacgccatat
     1081 tggtgtgatc cccgttatct acactacaag cgctcggcga tcggcctgcg cccgattgac
     1141 acccgcttgg ccctgcttca tggccggctg gtccgttagg acgcgcggga gagggtggcg
     1201 ggcgaccgcc gccgccgaag gagcaaccgc cccggaatct ctcaggcaaa aggaccgcgc
     1261 gcgcctggac gctctggaaa gcgtcctgct tcgcccaggc ttagcaggac caccgaaggg
     1321 gtaactccgc ccggcgccca ggcgtcggat acgggggaaa gctctcaggt ctccgtgaca
     1381 gagggggcat tggccggtcg ctcgatggaa cgatcgtgtc cggtgcggcc tttggagacc
     1441 gaagatgagc gacccgatga gcgacaatcc cgaacagccg cttccgctcg acgggtggca
     1501 ccgcgcccgc ggcgcccgga tggtgccgtt cgccggctat gccatgccga tccaatatga
     1561 tggcattttg gccgagcatc gctggacgcg gcaatcggcc ggtttgttcg acgtcagcca
     1621 tatggggcag atgatcctgt cgggcgcggg gatggacggg gggatggacg cggggctgga
     1681 cgcggcgctg gagagcgtgc ttccggccga tctgtcgctc ttgaaggacg ggcggctgcg
     1741 ctattcgctg ctgctgggcg acgagggcgg gatcatcgac gatctgatgg tgacgcgccg
     1801 gggcgaccat ttctacatgg tcgtgaacgg cgccaccaag catggcgata tcgagcatct
     1861 gcgggcgcgg ctgcccgaaa gcgtgcggct ggagcatctc gaggcgcagg cgctgctcgc
     1921 gctgcagggg ccgaaggcgg tcgaggtgct ggcgcggctg gcgccggggg tggacgcgct
     1981 gaccttcatg accggcggaa gcttcgagct ggagggcgtg cccgcctgga tcagtcgctc
     2041 gggctatacc ggcgaggacg gcttcgaaat ctccattccc gccgacgcgg ccgagcgggt
     2101 ggcggagcgg ctggcggccg agcatgaagt gcggccgatc ggcctcggcg cgcgcgattc
     2161 gctgcggctc gaggcggggc tgccgctcta cggccacgat ctcgacgcgg cgaccacgcc
     2221 catcgaggcc gatctcggct tcgccatctc caagcgccgg cgcctggagg gcggcttccc
     2281 cggcgccgcc cgcatccagc atgagctcga acatggcgcg atccgccgcc gggtcggcct
     2341 ggccgtgctc ggccggcagc cggtgcgcga gggggcgatg gtggtggacg gcgagggcaa
     2401 cgaggtcggc acggtgacca gcggcggctt ctcgccgatg ctggaggcgc cgatcgcgat
     2461 ggcctatgtg ccggccgcca tggccgaggc gggcgccgtc cggctcgccg cgcgcggcaa
     2521 gatattcgaa gcgaaggtcg tgccgatgcc cttcgtcccg caccgctacc accgcaaggg
     2581 agccgccaga tgacgcgtta cttcaccaag gagcatgagt ggatctcggt cgagggcgac
     2641 cgcgccaccg tcggcatcac cgatttcgcg caagggcagc tcggcgacgt cgtgttcgtc
     2701 gaagtgccgg ccgcgggcgc ccagctcaaa aagggcggcg aggcggcggt ggtcgagtcg
     2761 gtcaaggcgg cgtcggacgt cttcgcgccg gtcagcggcg aggtggtcga gggcaatcag
     2821 gcgctggtcg acgatcccgc gctggtcaac agcgacgcgg aaggcgaggg ctggttcttc
     2881 aagctcatcc tgtccgacaa tcacgagctt tcggggctga tgagccagga cgaatataag
     2941 gcttttgccg agagccagtg agccattccg ttcgtgtcga gcgaagtcga gacaaatatt
     3001 cgagcgtcag cgagaaggtg cctcggcttc gctcggcggg atgtctcgac ttc
//
LOCUS       DASVRK010000130         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_210165, whole genome shotgun sequence.
ACCESSION   DASVRK010000130 DASVRK010000000
VERSION     DASVRK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210165"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VF589_04860"
     CDS             <1..886
                     /locus_tag="VF589_04860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8256941.1"
                     /translation="LLQCHAPPPGKLVVARAERGGERAWLFLGLEQLRARAYVNWYSL
                     RFAAIGEGELVTAIARALKFGGVAQVELSPIERPESLRQSFRTAGWLTFLSPATASWQ
                     VGTEGLDFDTYWAKRPARLRNTAERKAKAAGLDIGVHCAFDEAAWADYERVYAASWKP
                     EEGSPAFLRALAEQEGAAGTLRLGIARKDGRAIAAQLWLVEGGRATIHKLAYAEEAKA
                     LSPGTILSMAMFRHVLDEDRVARIDYGTGDEPYKKDWMEERTILWRLTAFNPRTLVGL
                     AGAARAAASAIVRRGRTD"
     gene            908..1192
                     /locus_tag="VF589_04865"
     CDS             908..1192
                     /locus_tag="VF589_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8256942.1"
                     /translation="MGKSVPLTDEGEVDLTLRAVLVDVLGLPEARVAAFDPTTPLFGA
                     LPEFDSMAVAGLLTELEERLGILIEDHEVDADMLETYGSLLSFARAKALK"
     gene            1189..>1604
                     /locus_tag="VF589_04870"
     CDS             1189..>1604
                     /locus_tag="VF589_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256943.1"
                     /translation="MTHPGGLAYDRYSCHGHEEWLMRIGAADAQPILFLPPLFEEMNR
                     TRALLAATMRSLAARGFGCWLPDLPGTGESERALEACDWAHWTNAAAAAAERVAEASG
                     RRPAFASLRGGCLLDSEPPAPCRWRFAPVAGAALVR"
BASE COUNT          229 a          557 c          576 g          242 t
ORIGIN      
        1 gctgctccag tgccatgcgc cgccgcccgg caagctggtc gtcgcccgcg ccgagcgcgg
       61 cggggagcgc gcctggctgt tcctcggcct cgagcagctg cgcgcgcgcg cctacgtcaa
      121 ttggtacagc ctccgcttcg ccgcgatcgg cgagggcgag ctggtgacgg cgatcgcccg
      181 cgcgctgaag ttcggcggcg tcgcgcaggt cgagctgagc ccgatcgagc ggccggaaag
      241 cctgcggcaa tcgttccgca ccgccggctg gctgaccttc ctcagccccg ccaccgcctc
      301 gtggcaagtc ggcaccgagg gtctggattt cgatacttac tgggcgaagc ggccagcgcg
      361 tctcaggaac accgcggagc gcaaggcgaa ggccgcgggg ctcgacatcg gcgtccactg
      421 cgccttcgac gaagcagcct gggccgatta cgagcgtgtc tatgcggcga gctggaagcc
      481 ggaggaaggc tcgcccgcat tcctgcgcgc gctggccgag caggaggggg cggcggggac
      541 gctgcggctc ggcatcgccc gcaaggacgg ccgcgcgatc gccgcgcaat tgtggctggt
      601 cgagggcggc cgcgccacca tccacaagct cgcttatgcc gaggaggcca aggcgctgtc
      661 gcccggcacg atcctgagca tggcgatgtt ccgccacgtc ctcgacgagg accgcgtcgc
      721 ccggatcgat tatggcaccg gcgatgagcc ctataagaag gactggatgg aggagcggac
      781 gattttgtgg cggctgaccg ccttcaaccc gcgcaccctg gtggggctcg cgggcgcggc
      841 gcgggcggcg gcctcggcta ttgttcgccg cgggcgaaca gattagtcgc ttcgggcgac
      901 caaggggatg gggaagagcg tgccgctgac cgatgaaggg gaagtcgacc tgaccttgcg
      961 cgccgttctg gtcgacgtgc tcggccttcc cgaagcgcgg gtggcggcgt tcgacccgac
     1021 gacgcccttg ttcggcgcgc tccccgaatt cgactccatg gccgtcgccg gcctcctcac
     1081 cgagctcgag gagcggctcg gcatcctcat agaagatcat gaggtcgatg ccgacatgct
     1141 tgagacttat gggtctttgc tgagcttcgc ccgggccaag gcgctaaagt gacccatccc
     1201 ggcggtctcg cctacgaccg ctatagctgc cacggacatg aggaatggct gatgcggatc
     1261 ggcgccgccg acgcgcagcc gatcctgttc ctgccgcctt tgttcgaaga gatgaaccgc
     1321 acgcgggcgc tgctcgccgc gaccatgcgc agcctcgccg cgcgcggctt cggctgctgg
     1381 ctgcctgacc tgccggggac cggcgagagc gagcgagcgc tcgaagcctg cgactgggcc
     1441 cactggacca atgccgccgc cgccgccgcg gagcgggtgg cggaagcgag cggccgcagg
     1501 ccggccttcg ccagcctgcg cggcggctgc ctgctcgact ccgaaccgcc cgcgccctgc
     1561 cgctggcgct tcgctcccgt cgcgggcgcc gctctggtgc gcga
//
LOCUS       DASVRK010000131        23643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_521557, whole genome shotgun sequence.
ACCESSION   DASVRK010000131 DASVRK010000000
VERSION     DASVRK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 23643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23643
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521557"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3263
                     /locus_tag="VF589_04875"
     CDS             <1..3263
                     /locus_tag="VF589_04875"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8256944.1"
                     /translation="LVGSVAAINNALDGLSYAGPADSNGARDLTIGVSDNGFTGPNLI
                     FNPGGENGTAAADYATAVKPGGWDRVGGTGGLSVIQYATGGADPTLLNSGDDSAPIGG
                     GNDFFTGGPGGASSAVSQTIDVSGEAARIDGGDMIANMSGFFGGFMAQEDTMTMTVRF
                     LDANGDELDSAELGGPSAADRGNETKLLPYAGAASIPVGTRTIEVVLSATNSEGDSTD
                     GYADRLSLTLSELVTDTVSIDLAAANDPVGVAAGPDVSVAEEGSVAIGGLSISDVDAV
                     LAPDGEYTVTLSAADGTLTVGTPTGLDFTAGDGTGDATMTFHGTLAEVNAALATVSFT
                     GAEDFVGATDVDISVTDQTGAAVATGSGAATSATDSVGVTVTAVNDAPVIDLDSGAAG
                     SGATLAYTENGPAARIAPDATLTDPDSADYDGGSLEVEFTGNGDGDDRLTISDGGGIT
                     VAGGAVSYNGTEIGSVTGGTSGSDPLQISFNSAASVAAVQAVLQQVSYSNVSDTPSVL
                     ARTITYTLTDNDGGTETGTAVATVNVAATDDTGTAADDAFATDEATAITGENVLDNDD
                     DIDSTLEVGSVEGVAGNVGNQFALASGALVTLEADGTLSYDANGAFDHLAQTGSGAQN
                     VLATDSFSYTLTDGTSATVTITIDGVASDPHRMIGSSGNDDITGTSGVDWVRIEQGGD
                     DIARGQGGDDVFNLGAGFTAADAIDGGTGYDMLKLSGDYYTVPLVLGSDTLRRVEMIL
                     LSGGNSYDLTTHDDTVAAGRTLVVSGAALSPLEELWFDGSAETDGAFRLLGGGCNDFL
                     VGGARNDVLNGGIGTNLLAGGDGDDIYVVVGTGDTIVELAGEGFDAVRASVDYVLGDD
                     LEALLLLDTAVLGVGNALDNLVIGNGGDNKLRGGDGHDMLAGDGGADNLQGNAGDDSL
                     FGGAGADALFGSSGKDRLSGNNGADTLDAGTGDDRLYGGDANDILLAGIGNDTLFGGV
                     QRDTLTGGAGRDTFAFADSDSSALLAQADRITDFSAAQHDVIHLRQIDANENTVADDK
                     FSFIGTGAFSGAAGELRYQVVGGDALLQGDTDGNGIADFFIRVEGVTVLTSGDILG"
     gene            3334..3407
                     /locus_tag="VF589_04880"
     tRNA            3334..3407
                     /locus_tag="VF589_04880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3366..3368,aa:Gly,seq:tcc)
     gene            3603..5594
                     /locus_tag="VF589_04885"
     CDS             3603..5594
                     /locus_tag="VF589_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8256945.1"
                     /translation="MRKLAFLTATALAVLAGSPAAAEDYTYLIADRASGTMKVTQQGQ
                     ERSVAYSYNDRGRGHELKQIFRISSSGLLESTSVTGVDYLKVPVDEAFAVKEGKASWT
                     SRVDAGSVPAKGAFYIAHQRTPEHWAALARALRAAPGQSLDLLPTGRARLRRHGDLEV
                     SGDGGAREKVTLYAIEGVGVGATPVWLDSDGELFFTGASWAATVRKGFEKAAATLLAE
                     QGKFLSAALADAAKTTGRRPATALLIRNANLFDAKSTSMRAGTSVLIRGNRIEAVGLD
                     GAITVPAGAEIIDAGGKSLLPGLWDMHVHVGQDWAGLVYLGAGVTSVRDLAGDTDELM
                     ARRKRFDSGELVGPRVFMAGFIDGSGPMAGPSKVLAGTPEEIVRHVNRYADLGYEQIK
                     LYSSLKPELVPVAAKAAHARGMRVSGHIPAGMIAADALKAGFDEIQHANFVMLNFFPE
                     VAAETHSMKRLTVPAERGAGLDLQSPEVRSFIAELKAKDIVVDPTLSIFEVQFTAGPK
                     DPTPGLMPVLHRLPPLMARGAYGNGMAAGEAQLAQYRASWKKMVAMVGELHRAGVRLV
                     PGSDGFPGFTLHRELELWAEAGIPNLDILRAATLGAASVNKHDKELGSIEAGKLADLV
                     LVDGDPSKAISDVRKTQLVIKDGVIFDSEALYAQVGIRP"
     gene            complement(5646..5873)
                     /locus_tag="VF589_04890"
     CDS             complement(5646..5873)
                     /locus_tag="VF589_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256946.1"
                     /translation="MGGFVSESYYEVHIELANGFHRLLQGMAAGNVAGTGLGALERPA
                     ALQANSAVIPAQAGAGDDRRSIPLLLPGQEQ"
     gene            5872..6546
                     /locus_tag="VF589_04895"
     CDS             5872..6546
                     /locus_tag="VF589_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256947.1"
                     /translation="MTKGGIIVAAIAGASLPGGPIAQETGAGLAFWVVVIALLQLVTI
                     ILGLFVIKKAVGDTGKAIAAMRDANALAEQSARRQLRAYLVIEPGGVNEVVDGFARLP
                     LQVENKGQSPAFDVRLFANHAVVENPRDISLDDLGVPLGGEIDGASLGPGVCRSIYSY
                     ASEALIEPYAYDIVRKRRAIIQFGYVGYRDAFGDEWRTNFAFYHWGNNLTPANALRCR
                     FGNDAT"
     gene            6712..7455
                     /locus_tag="VF589_04900"
     CDS             6712..7455
                     /locus_tag="VF589_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256948.1"
                     /translation="MDSEIALSALVAVAFAAVHVFGGRLNFLRVTPRSVWLSAAGGVS
                     VAYVFVHLLPELAEHQETLAEGLPGLLGGIEKHSYLLSLIGLMTFYGLDRLASCAPRP
                     AAREADDLEVPSGVFWIHLGSFALYNLLIGYLLLHREGTDRFGLLTYGTAMALHFLVN
                     DQGLRDQHGRSYARTGRWLLAAAPLAGWGLGLATAISPPVIAAMFAFLAGGIVLNVLK
                     EELPEERESRFSAFAAGTIGYAALLLATS"
     gene            complement(7532..8944)
                     /gene="glnA"
                     /locus_tag="VF589_04905"
     CDS             complement(7532..8944)
                     /gene="glnA"
                     /locus_tag="VF589_04905"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX8256949.1"
                     /translation="MAIDAGSILQRIKDEEIEWVDLRFTDPKGKWQHLSMVSGLIDED
                     QLEDGFMFDGSSIAGWKAINESDMILKPDLDAAYVDPFSATPMLILFCDIVDPGSGEL
                     YARDPRSTAKRAEAYLKTTGIGDTVYVGPEAEFFMFDDVRFEDGYNSSSFRIDDIELP
                     TNTGREYENGNLGHRPRAKGGYFPVAPVDSAMDIRAEMVSTMIEMGLPMDKHHHEVAA
                     GQHELGMTYGKLVETADRMQIYKYVVHMVAHAYGKTATFMPKPIKSDNGSGMHTHFSI
                     WEGGKPLFAGNGYAGLSETALFFIGGVIRHARALNAFTNPTTNSYKRLVPGFEAPVLL
                     AYSSRNRSASCRIPYGAGEKAKRVEFRFPDALANPYLAYAAILMAGLDGIQNRIHPGD
                     PIDKNLYDLPPAELVNVPTVSGSLREALIALQNDRAFLTRGDVFSDDQIDAYIELKWE
                     EQMRWETTPSPVEYDMYYSS"
     gene            complement(9216..10271)
                     /locus_tag="VF589_04910"
     CDS             complement(9216..10271)
                     /locus_tag="VF589_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256950.1"
                     /translation="MATITGTADGETLSGTRLQDDIFGLGGDDTIRGRAGNDTIDGGT
                     EDDLLIGRTGSDTYWVDSELDKVVERLGEGETDTVFATISFTLRRNIENLELRGEAAR
                     GYGNDLDNRLTVEEGQVVRNVLNGRGGADFMSGGRGRDIYYVGDAGDVVDEIGIDEDD
                     TVRSTISLDIGQFDKPSESVYVENLFLLGSADIDGTGGGVQNRIVGNSGDNEIRGMQG
                     ADVLQGRDGNDSLYGGVDSGMDVLIGGEGQDGFYFEQYATIPYRDIINDFTAADDTIY
                     LDRSDFTEMPLGELPESAFVRGSVPQDADDRILYDSDTGQIRYDPDGTGEQEAAFFAV
                     VAPGTVLNANDFVGYGG"
     gene            complement(10311..11378)
                     /locus_tag="VF589_04915"
     CDS             complement(10311..11378)
                     /locus_tag="VF589_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8256951.1"
                     /translation="MATITGTAAGETLNGTRLQDDIFGLGGDDTIRGRAGNDTIDGGT
                     EDDLLIGRTGSDTYWVDSELDKVVERLGEGESDAVFATISFTLRRNIENLELRGEAVR
                     GDGNDLDNRLTVEAGQDVDNVLNGRGGADFMSGGHGDDTYYVDDVNDFIEELGQGLDT
                     VRSTVSFNVGVFDQTVIEDIYVEDLVLLGTAVIDGTGGRTDNRIVGNSADNVLMGMGG
                     SDVLNGRGGNDSLYGGRGFDTLTGGAGEDGFYFELLGFDKKIRDFTSADDTIYLDRDA
                     YTEIPEGVLPESAFVRGSGPFVGGSDAQDADDRIIYYTTEDHPSAFVYYDPDGTGEME
                     KTLLFVVPTGTEIQASDFIGY"
     gene            11584..14700
                     /locus_tag="VF589_04920"
     CDS             11584..14700
                     /locus_tag="VF589_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256952.1"
                     /translation="MIDVVDLASLDGKPVSAGDFITVGGFSGGVFEARSSSSVLEASK
                     VTLRFLPRPFTANLTVWDHVWDAETEKGATNPGGKHGDTITIDGHQITLVSSAPTGQQ
                     VQIGIGATDALKAADLAEKIMSYVNVNSVLIGVRMRGTTLLRCSANDWGQRFVGGVAS
                     PVAVSVSSTDAHPPFTWDVTHLSDPSEGSSVRFGDQPTDVLTFKAPPVIGANREIAIG
                     ATPSATAQNFKKYINERSETLDLTASGEVTDAAKGMDITLTPSSFAGLARLRNFVKGG
                     LNVTNAGVVTPKPPECDLFVVQAMGAAAADKLGAIYRPSTASPTVVNVRVSVERDGTL
                     HPSFWDLKGERGHDGDYIQPRMNAMFDAAHFLGCRIELRAGANYDLTRGDGYTNRTQM
                     DAIRFRNISLYGNGATISNRNDIKRSATPSTPGEAGEQNSIFYFGVGHPAFDSDVPFA
                     RIVAAAKGDTYVELVLLNTGSYFRVGDVIPIMTVAGRSARTVWHPVAREFYKIVHIDG
                     KILHLDRGLASDYPLNSRVALWMGHYDSTIRQDIFIADNPRVRDLSLYSPYGGAMTRS
                     QAYNVDFEFDLLEAYYGVFINAMNGHVKVKKVRAKKKACDLAHNSQGVTVDMDEVEWW
                     PNDTSDDAPMMHFAENSRDFVITCKLLRCMNSADEPDFPGPQIINGTDGARGIIKLGM
                     SIVEKMLNGVTEKGDWATATAYQINDIVAVGSKKYVARKAHTSSADFDAKEELNWAPQ
                     VRRQFFTLLHKDSLRGEAPHHFTDLDLDLGQVFYGDAPGALVRIHKNTVNPLLGPKRI
                     RVSGGRFNGAEPLVNLGAHVEGDDCSIAEGVHIPGRLRVDGNGGVYGGHFTQGYSGES
                     VGAPTRVAISSDMTPQLTKRVVRSIGINSTDTANTYAPGTLILPPGTLGQDGDALELT
                     IAYETSTGAGSKNIQLFGQSTGAAVQLAAILLPANAVALGEAVFSLRRAAIGELFASV
                     FDRQGNAPPQAGMRVLPNLPVLNVNTDPNLDVDTITLSVRIFIGAASGGFIIPRVLTW
                     RPLLSWHV"
     gene            complement(14716..14925)
                     /locus_tag="VF589_04925"
     CDS             complement(14716..14925)
                     /locus_tag="VF589_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8256953.1"
                     /translation="MDDPLLSSMSPDELRSAMRTLGYRTQNDLAGAIGVSRSTVSLWL
                     EGKVGVPRPVAMLLRMLVAAQRRPF"
     gene            complement(14995..15333)
                     /locus_tag="VF589_04930"
     CDS             complement(14995..15333)
                     /locus_tag="VF589_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX8256954.1"
                     /translation="MKKIEAIIKPFKLDDVKEALHEVGVSGITVTEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEVVVEDGLAERAVEAIATAARTGRIGDGKIFVIDVQEALRI
                     RTGERGSDAI"
     gene            15514..15969
                     /locus_tag="VF589_04935"
     CDS             15514..15969
                     /locus_tag="VF589_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256955.1"
                     /translation="MGIEVLDGVVETAAVKRSSGKLTIYDSLTIRLKDGGERRLEKVA
                     AAPAVAAALQPGVEGRFYGYKAIDHRGMIGVRTRDGRAAFAIPSGNERIMLMLAIAGL
                     AWFLIAFFARDSITFLGLIGGVLGAIGYVRYRQTRIAGRALYDADAGYA"
     gene            complement(15981..16184)
                     /locus_tag="VF589_04940"
     CDS             complement(15981..16184)
                     /locus_tag="VF589_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256956.1"
                     /translation="MTISVLLRCDRCRGDSLSIPLEGGDETEIDCEDCGASLGTLADL
                     KTMVSLKVVGRKHAKRPAFSHWG"
     gene            16328..16675
                     /locus_tag="VF589_04945"
     CDS             16328..16675
                     /locus_tag="VF589_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256957.1"
                     /translation="MAKAIGAALLWGALLLGGCVSIGGPRLGSELVGRSARLVPARGD
                     ASMLHFAPDGTVESTFGRRSASGRWWIKNRRLCFLWAGSFRECWPYGAPLQPGRPRSI
                     RSDRGNVVHVTLL"
     gene            complement(16683..17516)
                     /gene="map"
                     /locus_tag="VF589_04950"
     CDS             complement(16683..17516)
                     /gene="map"
                     /locus_tag="VF589_04950"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX8256958.1"
                     /translation="MTEYRTIQRYDVAPAPERGGAIKLHGPEGFAGMRAAGRLAAEIL
                     DGLVPHVVPGVTTQALDDIVSAMMRDAGAVAATLGYRGYTHSSCTSINHVVCHGIPGD
                     RVLRDGDIVNIDVTPILGGWHGDSSRMYLVGDVPVKARRLVDVTYECLMLGLEQARPG
                     NHLGDISHAIQSHAERHRYGVVRDFCGHGLGQLFHDSPEVVHVGRPGTGPELRPGMFF
                     TVEPMINIGKAACKVLEDGWTAVTRDRSLSAQFEHSIGITEDGCEIFTKSPKGLDRPP
                     Y"
     gene            17746..18063
                     /locus_tag="VF589_04955"
     CDS             17746..18063
                     /locus_tag="VF589_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256959.1"
                     /translation="MEEAAPAASFEEARQLLEARLNQIEDAHSGVPYCPENWRDDGRM
                     YPPQDDSERHSPIAGVRVFRTRGHRVSIAANGAIRITTAGTLQERIVLDKAGKDGGSC
                     PAA"
     gene            18071..18589
                     /locus_tag="VF589_04960"
     CDS             18071..18589
                     /locus_tag="VF589_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8256960.1"
                     /translation="MTVFDQLVDRVRQRFDTAQDVSVSVEQPARPDGTYFLTIACDDY
                     AVEVEWQRNRGFGIAAGRDLVFGSGVDEVHGSAAAAFDRIEHLLQTRESTSGEASVNL
                     IDLRKLRGLLQKDVAEQLGISKSGVAQMERAESLTTMQIDTLRKLIATLGGELVLSAR
                     FPGGRERRIAVD"
     gene            18641..19399
                     /locus_tag="VF589_04965"
     CDS             18641..19399
                     /locus_tag="VF589_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX8256961.1"
                     /translation="MASERIWTAGLLIIGDEILSGRTEDRNIAPIARWLNLQGIRLGE
                     VRVVPDVEARIVAAVNALRTAHDYLFTTGGIGPTHDDITVDAIAAACGVGVEVHPKAR
                     AALEAYYASRGGLSEARLRMARVPAGADLIENPMSGAPGIRFGNIFILAGVPHIAALM
                     LEALSGRIEGGRPLLSRTIGCWVPESEIAETLRDTERAHEGCQIGSYPFFREGRVGAN
                     FVIRSTEAAALDACVEDLIGRLEAAGYAVARDGI"
     gene            complement(19450..20829)
                     /locus_tag="VF589_04970"
     CDS             complement(19450..20829)
                     /locus_tag="VF589_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX8256962.1"
                     /translation="MAEAPQPLRSGSLGGALANLRWLLGSRGVNAILSLFYIAIVTRS
                     LGAAGFGQFALVTGTGGAIVTIISFQTWQIIVRYGTEHLHAGRPDRLARLLKGALALD
                     AAGALLGSLIAMAAVAALAPAFGWSPDMRRDTFLFCCAALLSMRSTPTGILRLHDRFA
                     AAAFADTALPVVRLAGAGLVLAAGPSVRGFLIAWAAAELASSIAFWVSAARVARGLPW
                     RRAPLSLPALRADNPGILHFAAITNSAQTLTLATRQLPVLLVGFFASPAAAGAYRLAH
                     QLGRAMARVSQLFARALFPELMRIRTAAGSSAEFGPMIARIVRTAALGGGLLLLLLLL
                     AGRPLLELVAGPEFAAAYPLLLLLGAAAVVDLIGVAFEPALIASGQAGRSFRVQLIAA
                     AALIGLMLVLIPQAGTIGAAAAVLAGSLLGFALMAVAVRRVLRRRPGAEEAALAASLE
                     AHPPRHNDH"
     gene            complement(20837..22024)
                     /locus_tag="VF589_04975"
     CDS             complement(20837..22024)
                     /locus_tag="VF589_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HEX8256963.1"
                     /translation="MTRAQHLYYRLKKVFALYPGVLRPTSAGFLDEHYRTRRAQRSPV
                     RAALDALAGGVFHLWVPYRARSIARKYGLGRDWARTAARIAHRRFADPNDIALFRVES
                     EADLDGFIRRFEHADVSKRINPKNWAAGCVLADKRLFYARCAELGLPHPPLLATIVDG
                     AVAVREAPTVAALALKPVDGEGGSGFQLFDYPAADGDAAAFARFVRERFGRLPGAWLI
                     QPKVSVHPDLRDLSLSALSTARVSTMINERGEPEIVTSVLRFPSDPNSQVDNIKSGGL
                     MAPIDLATGLLGPACRGRWVGDVTEHPASGAAIAGRRLPFWEEAKALVLKAHRGGFAD
                     YNLVGWDVALTAEGPLLIEGNGKPCMIVAQRAGRRGVGHQRFGELIAHHLKHPPRPRA
                     PRA"
     gene            complement(22136..23347)
                     /locus_tag="VF589_04980"
     CDS             complement(22136..23347)
                     /locus_tag="VF589_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HEX8256964.1"
                     /translation="MSWVIRVAKPARIARGRRGSAHPVDLYHWRLWSHWPWRRRLAAL
                     AMNLPQAALALTGLGQAGSLRRAVRDLADALKWNVHPTELRVARESGVGGAAGALIGN
                     YDKKGINLLLNPRAFPKRDSILFDKARFAAFIARQSGMTGIPTLAQGDLAAGLPAWIE
                     AAGELIFKPGFSSRGRGIFGLRRHGLGWVAERGGREQPVADLLAWAQSALGPGDVLQQ
                     RLRTHEALAALSPGALPTLRLVTCLDEFGAIELVDATLRLGRGSVIVDNFSAGGLAAP
                     VDLATGVAGPARSKSSRWRIGSHDVHPSTGAPIAGFALPGWDEAKRQAVLGHRALLGE
                     DFLLVGWDVALAEDGPVILEGNWNPGGNLLQTVSGRGIAATRLGALYRHHLARLPDAR
                     FRDAPVLTFSR"
     gene            complement(23412..>23643)
                     /locus_tag="VF589_04985"
     CDS             complement(23412..>23643)
                     /locus_tag="VF589_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256965.1"
                     /translation="IPARCSLPSSCSGRSLLLAGPALRWARKLRLVPAGPRAIAGYAL
                     LACSGFALLFIASTADLLARFTPWRWGWRWRC"
BASE COUNT         3967 a         7718 c         8108 g         3850 t
ORIGIN      
        1 ccctggtggg cagcgtcgcc gcgatcaaca atgcgctcga cgggctctcc tatgccggcc
       61 ccgccgactc gaacggcgcc cgcgacctta ccatcggcgt cagcgacaat ggctttaccg
      121 gcccgaacct gatcttcaac ccgggcggcg agaacggcac cgcggccgcc gattacgcca
      181 ccgcggtgaa gccgggcggc tgggaccgcg tcggcggcac cggcggcctc agcgtcatcc
      241 aatatgcgac cggcggggcg gacccgacgc tgctcaattc gggcgacgac agcgcgccga
      301 tcggcggcgg caacgacttc ttcaccggcg gacccggcgg cgcctcctcg gcggtcagcc
      361 agaccatcga cgtcagcggc gaggcggccc gcatcgacgg cggcgacatg atcgccaaca
      421 tgagcggctt cttcggcggc ttcatggccc aggaagacac gatgaccatg acggtccgct
      481 tcctcgacgc gaacggcgat gagctggact cggcggagct tggcggcccg agcgcggccg
      541 accgcggcaa cgagaccaag ttgctgccct atgccggggc cgcgtcgatc ccggtcggca
      601 cccgcaccat cgaggtcgtg ctgagcgcga ccaacagcga aggcgattcc accgacggct
      661 atgccgaccg gctgtcgctg accctgtccg agctcgtcac cgacaccgtc agcatcgacc
      721 tcgccgcggc caacgacccg gtcggcgtgg cggccggtcc cgatgtctcg gtcgccgagg
      781 aaggcagcgt cgccatcggc gggctttcga tcagcgacgt cgacgcggtg ctcgcgccgg
      841 acggcgaata taccgttact ttgagcgcgg ccgacggcac gctgaccgtg ggcacgccca
      901 ccggcctcga cttcaccgcc ggcgacggca ccggcgacgc caccatgacc ttccatggca
      961 cgctcgccga ggtcaacgcc gcgctcgcca cggtgagctt caccggggcc gaggatttcg
     1021 tcggcgccac cgatgtcgac atatcggtga ccgaccagac cggcgccgcc gtcgccaccg
     1081 gatcgggcgc cgccaccagc gccaccgaca gcgtcggcgt caccgtgacc gcggtcaacg
     1141 acgcgcccgt catcgacctc gattccggcg ccgccggcag cggggcgacg cttgcctata
     1201 ccgagaacgg cccggccgcg cggatcgcgc ccgacgcgac cctcaccgat cccgactccg
     1261 ccgactatga cggcggctcg ctcgaggtcg agttcaccgg caatggcgac ggcgacgacc
     1321 ggctgacgat cagcgacggc ggcggcatca ccgtcgccgg cggcgcggtc agctacaatg
     1381 gaacggagat tggaagcgtc accggcggca ccagcggctc ggatccgctc cagatcagct
     1441 tcaactcggc ggcgagcgtc gccgcggtgc aggcggtgct gcagcaggtc tcctattcga
     1501 acgtgtcgga cacgccgtcg gtgctcgcgc gcaccatcac ctacacgctc accgacaatg
     1561 acggcggcac tgagaccggc accgcggtcg ccaccgtcaa cgtcgccgcc accgacgata
     1621 ccggcaccgc cgccgacgat gccttcgcca ccgacgaggc gaccgcgatc accggcgaga
     1681 acgtgctcga caatgacgac gatatcgact cgacgctgga ggttggctcc gtcgagggcg
     1741 tcgccggcaa tgtcggcaac cagttcgcgc tcgcctcggg cgcgctggtc acgctggagg
     1801 cggacgggac gctgagctat gacgccaacg gcgccttcga tcatctcgcc cagaccggct
     1861 ccggagcgca gaacgtcctg gccaccgaca gcttcagcta cacgctcacc gacggcacgt
     1921 cggcgacggt caccatcacc atcgatggcg tcgcttccga tccgcaccgg atgatcggca
     1981 gcagcggcaa tgacgacatt accggcacca gcggcgtcga ctgggtccgg atcgagcagg
     2041 gcggcgacga catcgccagg ggccagggcg gcgacgacgt gttcaacctt ggcgcgggct
     2101 tcaccgccgc cgacgcgatc gacggcggca ccggctacga catgctgaag ctcagcggcg
     2161 actattatac cgtgccgctg gtgctcggct ccgatacgct gcggcgggtc gagatgatcc
     2221 tgctgtccgg cggcaacagc tacgatctca ctactcatga cgacactgtc gcggccggcc
     2281 ggactttggt cgtatccggc gcggcgctca gcccgctcga ggaattgtgg ttcgacggct
     2341 cggccgaaac cgacggcgcg ttccggctgc tcggcggcgg ctgcaacgac ttcctcgtcg
     2401 gcggcgcccg caacgacgtg ctcaacggcg gcatcggaac caacctgctg gccggcggcg
     2461 acggcgacga catctatgtg gtggtcggca ccggcgacac gattgtcgag cttgccggcg
     2521 agggcttcga cgcggtgagg gcgtcggtcg actatgttct cggcgacgat ctcgaagccc
     2581 tgctgctgct ggacacggcc gtgctcggcg tcggcaacgc cctcgacaat ctcgtcatcg
     2641 gcaatggcgg cgacaacaag ctgcgcggcg gcgacggcca cgacatgctg gccggcgacg
     2701 gcggcgccga caatctgcag ggcaatgccg gcgacgattc gctgttcggc ggggccggcg
     2761 ccgacgcctt gttcggaagc agcggcaagg accggctgtc gggcaacaat ggcgccgata
     2821 cgctcgatgc cggcaccggc gacgaccggc tctacggcgg cgatgccaac gatattttgc
     2881 tcgccggcat cggcaacgac acgctgttcg gcggcgtcca gcgcgacacg ctgaccggcg
     2941 gcgccggccg cgacacgttc gccttcgccg acagcgacag cagcgcgctg ctggcccagg
     3001 ccgatcggat caccgacttc tcggccgcgc agcacgacgt catccacctc cgccagatcg
     3061 acgccaacga aaatacggtc gccgacgaca agttcagctt catcggcacc ggcgcctttt
     3121 cgggcgccgc cggcgagctg cgctaccagg tggtcggcgg cgacgcgctg ctgcagggcg
     3181 acaccgacgg caacggcatc gcggatttct tcatccgcgt cgaaggggtg acggtgctga
     3241 cgtcgggcga tatcctcggc tgaggcgtcg gcggcgttga cgagcccccg ccggccgtgg
     3301 catcggccgt ggcatcggcg gccgccatgg cgcgcgggta tagctcaatg gtagagcact
     3361 agccttccaa gcttgtgacg cgggttcgat tcccgctacc cgctccaccg caatcccata
     3421 gcagatgatt tcgggtccat cgcgggcggc aaataggccg acttcttcct ggtgccgggc
     3481 gatttcacgc gcgacctgga ggcgatcaac gtgaccttcg gcacctagca acccgctcac
     3541 actgtggcag cattcccgtc atgacccgcc aaaagagtgg cggataagga gggatcaggt
     3601 tgatgaggaa actagcgttt ttgaccgcta cggcactggc cgttcttgca ggctccccag
     3661 cggcagccga agactatacc tacctgattg cggaccgggc gtcgggcacg atgaaggtga
     3721 cgcagcaggg gcaggaacgc tcggtcgcct atagctataa cgaccgcggc cgcggccatg
     3781 agctgaagca aatctttaga atatcctcga gcgggcttct tgaatccact tccgtcacag
     3841 gcgtcgacta tctgaaggtg ccggtggatg aagcgttcgc ggtcaaggaa ggcaaggcaa
     3901 gttggaccag ccgtgtcgat gctggctcgg ttcccgccaa gggagctttc tacatcgccc
     3961 atcagcgcac gccggagcat tgggcagcgc tcgcgcgcgc gctgcgagct gcgcccggac
     4021 aatcgctgga cttactgccc acgggacggg cgaggctgcg caggcacggg gacctcgagg
     4081 tctccggaga cggcggcgcc agggagaagg tgacgctgta cgccatcgag ggggttggcg
     4141 tgggcgcaac ccccgtttgg ctggattccg acggcgagct gttcttcacc ggcgctagtt
     4201 gggcggccac ggtccgcaaa ggtttcgaga aagccgccgc gacgctcctc gcggagcagg
     4261 gtaaattcct tagcgcggcc ctggccgatg cagcaaaaac cacagggcgc cgtcccgcga
     4321 cggcgctgct catccgcaac gcgaacctgt tcgatgcgaa gtctacgtcc atgcgtgccg
     4381 gaacgagcgt gcttatccgc ggcaaccgca tcgaggctgt ggggctggac ggggcgatca
     4441 ctgtccccgc cggtgccgag attatcgatg cgggcggcaa aagcctactt cccggcctct
     4501 gggacatgca cgtccacgtg gggcaggatt gggcgggtct cgtttatctc ggcgccggcg
     4561 ttacgagcgt tagggacctt gccggcgaca cggacgaatt gatggcgagg cgtaagcgct
     4621 tcgacagcgg cgagcttgtc ggaccgcgcg tcttcatggc cggtttcatc gacggttcgg
     4681 ggccgatggc ggggccctcg aaggtgctcg ccgggacgcc tgaagaaatc gtccgacacg
     4741 tgaaccgtta tgcggacctc ggctacgagc agatcaagct gtacagctcc ctgaagccgg
     4801 agctggtgcc ggtagcggcc aaggcagccc acgcgagggg catgcgcgtc tccggccata
     4861 ttcccgcagg catgatcgcg gccgatgcgc tcaaagccgg tttcgatgag atccagcacg
     4921 ccaatttcgt catgctgaac ttctttcccg aagtcgccgc cgagacccac agcatgaagc
     4981 ggctcacggt gccggcggag cgcggagccg gattggatct gcaatcgccc gaggtccgat
     5041 ctttcattgc cgagctgaag gcgaaggaca tcgtcgtcga tccgacgctt tcaatcttcg
     5101 aagttcaatt caccgcaggt ccgaaggatc cgacgccggg gctcatgccg gttttgcacc
     5161 ggctgccgcc gctgatggcc cgcggcgcct atggtaacgg catggccgcc ggcgaagcgc
     5221 agctggcgca atatcgggct tcgtggaaga agatggtggc catggtgggc gagcttcacc
     5281 gggcgggggt tcggctcgtt ccgggaagcg acggcttccc gggcttcacg ctgcacaggg
     5341 aattggagct atgggcggag gccggcattc ccaatctcga catcctgcgc gccgcgacgc
     5401 tcggcgccgc ctccgtcaac aaacatgaca aggagctcgg ctcgatcgaa gcaggaaagc
     5461 ttgccgacct ggtgctggtc gacggcgatc cttcgaaggc gatttcggat gtgcgcaaga
     5521 ctcagctcgt gatcaaggac ggcgtgatct tcgattccga agcattgtac gcacaagtcg
     5581 gtatccgacc gtgacccgct ccaccgcagt ggcaacctcc cgtcatccca gcggaagctg
     5641 ggatctcact gttcttggcc gggaaggaga agcgggattg acctgcggtc gtctccggct
     5701 ccagcttgcg ctggaatgac ggcggagttg gcttggagcg cggccggccg ctcgagcgcg
     5761 cccagccctg tgccggccac gttaccggcg gccatgccct gcaacagacg gtgaaagccg
     5821 tttgccagct caatatggac ttcatagtag ctttcagaga caaagccgcc catgacgaag
     5881 ggcggcatca tcgtggcggc gatagcaggt gcctcgctcc ccggcggtcc catagcccaa
     5941 gagaccgggg cgggccttgc cttctgggtc gtcgtcatcg ccctgttgca gctcgtgact
     6001 atcatcctcg gcctgttcgt tatcaaaaag gcggtagggg acaccggcaa ggcaattgcg
     6061 gccatgcgcg atgccaacgc cctcgccgag caatccgcga ggcgccagct gcgcgcctat
     6121 ctggtgatcg agcccggcgg ggttaacgag gttgtggacg gcttcgcccg cttgccgcta
     6181 caggtagaga ataaagggca atcccccgcc ttcgacgtaa ggctgttcgc gaaccacgcg
     6241 gtagtcgaaa acccccgaga catcagcctg gacgatctcg gagttccgct gggcggggag
     6301 atcgatggcg catcgctggg gccgggcgtc tgtcgctcga tctatagcta tgcgagcgag
     6361 gccctgatcg agccctacgc ctatgacatc gtgagaaagc gacgcgccat tatccaattc
     6421 ggttacgtcg gttaccgcga tgccttcggc gacgagtggc ggacgaactt cgctttctat
     6481 cattggggaa ataatctcac ccccgcaaat gcgctgcggt gccgcttcgg caatgatgcc
     6541 acataggtcc aacgacgtcg aacatcatgg tacacgttcg gagggaatag cccctgccga
     6601 ctcacccgat cgcgcgcctc ccggcggttg ggaggctccg tcgacggtct gagcacccgg
     6661 tctccgtccc cggcgcgcgg aacagttacg cccgcgccgc gctctcgcct tgtggacagc
     6721 gagatcgccc tgtcagcgct cgtcgcggtc gcttttgcgg cggtgcatgt gttcggcgga
     6781 aggctgaact tccttagggt gacgccgcgc agcgtctggc tgtccgccgc tggcggcgtc
     6841 tcggtcgcct atgtgttcgt tcatctgctg ccggagctgg ccgagcatca ggagacgctg
     6901 gccgaagggc tgcccgggct gctcggcggc atcgaaaaac attcctacct tctgtcgctg
     6961 atcgggctga tgaccttcta cggcctcgat cgcctcgcaa gttgcgcccc gcggccggcc
     7021 gcgagggagg ctgacgacct cgaggtgccg agcggcgtgt tctggatcca ccttggttcg
     7081 ttcgcgctgt acaatctgct gatcggctat ttgctgctcc accgcgaggg gaccgaccgg
     7141 ttcgggctgc tcacctacgg gacggcgatg gcgctgcact tcctggtcaa cgaccagggc
     7201 ctgcgcgacc agcatggccg gagctacgcg cggaccgggc gctggctgct cgcggccgct
     7261 ccgctcgccg gctggggctt ggggctggcc acggcgattt cgccgccggt cattgccgcc
     7321 atgttcgcgt ttctcgccgg cggcatcgtc ctcaacgtgc tgaaggaaga gctgccggag
     7381 gaacgggaaa gccgcttctc cgccttcgcc gccgggacga tcggctacgc ggccctgctg
     7441 ctggcgacgt cgtgagaagc tgggtgctgg cgatgcggct gagcgtcaag gcccgctacc
     7501 cgttcaccgc gacgctgccg acgaccctcg ctcagctcga ataatacatg tcatattcga
     7561 ccgggctcgg cgtcgtctcc cagcgcatct gctcttccca tttgagctcg atataggcgt
     7621 cgatctggtc gtcggagaag acgtcgcccc gagtcaaaaa ggcgcggtcg ttctggagcg
     7681 cgatgagggc ttcgcggagc gagcccgaca cggtcggcac gttgaccagc tcggccggcg
     7741 gcagatcgta aaggttcttg tcgatcgggt cgccgggatg gatgcggttc tggatgccgt
     7801 cgaggccggc catcaggatc gcggcataag cgagataggg gttggcgagc gcgtcgggga
     7861 agcggaactc gacccttttc gccttttcgc cggcgccata agggatgcgg caggaggcgg
     7921 agcggttgcg gctcgaataa gcgagcagga ccggcgcctc gaagcccggg accaggcgct
     7981 tgtagctgtt ggtggtcggg ttggtgaagg cgttgagcgc gcgcgcgtgc cggatcaccc
     8041 cgccgatgaa gaacaaggcg gtctcgctga gcccggcata gccgttgccg gcgaacagcg
     8101 gcttgccgcc ttcccagatg gaaaagtgcg tgtgcatgcc cgagccgttg tccgacttga
     8161 tcggcttggg catgaaggtc gcggtcttgc cataagcgtg ggcgaccatg tggacgacat
     8221 atttgtagat ctgcatgcgg tcggcggtct cgaccagctt gccgtaggtc atgccgagct
     8281 catgctggcc ggcggcgacc tcgtgatgat gcttgtccat cggcaggccc atctcgatca
     8341 tggtcgagac catctcggcc cttatgtcca tcgcgctgtc gaccggcgcg accgggaaat
     8401 agccgccctt ggcgcgcggc cggtggccga gattgccatt ctcatattcg cggccggtat
     8461 tggtcggcag ctcgatgtcg tcgatccgga acgaggatga attgtagccg tcctcgaagc
     8521 ggacgtcgtc gaacatgaag aattcggctt cggggccgac atagacggtg tcgccgatgc
     8581 cggtggtctt gagataggcc tcggcccgct tggcggtcga gcgggggtcg cgggcgtaaa
     8641 gctcgccgct gccgggatcg acgatgtcgc agaacaggat cagcatcggg gtggcgctga
     8701 acgggtcgac ataggcggcg tcgaggtccg gcttcaggat catgtcgctt tcgttgatcg
     8761 ccttccagcc ggcgatcgac gagccgtcga acatgaagcc gtcctcgagc tggtcctcgt
     8821 cgatgaggcc ggagaccatc gacagatgct gccacttgcc cttggggtcg gtgaagcgca
     8881 ggtcgaccca ctcgatctct tcatccttga tccgctgaag gatgctgccc gcgtcgattg
     8941 ccatctgcct tacccttttg cctgaatgcg cgcccgcgcg aatccgcatc cgcacgtcat
     9001 gactagcgat tccagcgcgc gggctccgcg tccagccgcg aatgcggacg ggttaagaat
     9061 tcacgtggac agctcgtctc ccggggtcgg tcggccgggc aatacaggcg cgtgtcgccg
     9121 agcgtctcgg ggcgcagccg ttgctatgag gtcgccgggc cttcaatccg ccggagcgcc
     9181 ccgggccgcg ggcagtgccc gcggccccgc gggcctcagc cgccgtagcc gacgaaatcg
     9241 ttggcgttca aaaccgtgcc gggagcgacg accgcgaaga aagcggcttc ctgctccccg
     9301 gtgccgtccg gatcatagcg gatttggccg gtatcgctgt cgtagaggat gcggtcgtcg
     9361 gcatcctgcg gcaccgagcc gcggacgaag gcgctttcgg gcaactcgcc aagcggcatt
     9421 tcggtgaagt cgctgcgatc gaggtagatg gtatcgtccg ccgcggtgaa atcgttgatg
     9481 atgtctctat aaggaatggt cgcatattgc tcgaagtaga agccgtcctg accttctccc
     9541 ccgataagaa catccattcc ggaatcgacg ccgccataga gcgagtcgtt gccatccctt
     9601 ccctgcagaa cgtccgcgcc ctgcatgcca cgaatttcat tatcaccgga attcccgaca
     9661 atgcggttct gtaccccccc gccagtgcca tcgatatcgg ccgatccgag caggaagaga
     9721 ttctcgacgt atacggactc agagggctta tcgaactgtc ctatgtccaa actgatggtg
     9781 cttcgaaccg tatcgtcttc gtcaatgccg atttcatcga ccacgtcgcc ggcatcgccg
     9841 acataataaa tgtcccggcc ccggccgccg ctcatgaaat cggccccgcc gcggccgttg
     9901 aggacattgc ggacgacctg gccctcctcg accgtcagcc ggttgtcgag gtcgttgccg
     9961 tagccgcgcg ccgcctcgcc gcgaagctcg agattctcga tgttgcgccg gagggtgaag
    10021 gagatggtgg cgaacaccgt gtcggtttcg ccctcgccga gccgctcgac gaccttgtcc
    10081 agctcggaat cgacccaata agtatcgctg ccggtccgcc cgatcaggag atcgtcttcg
    10141 gtgccgccgt cgatcgtgtc gttgccggcc cggccgcgga tcgtgtcgtc cccgccgagg
    10201 ccgaaaatgt cgtcctggag ccgcgtcccg ctcagtgttt cgccatcggc agtgcccgta
    10261 atcgtcgcca ccgcggttct ccccatgcgc tgccggaaag ccgccccggc tcaatagccg
    10321 atgaagtcgc tcgcctggat ctccgtcccg gtcggtacga cgaagagaag cgttttttcc
    10381 atctctccag taccgtccgg gtcgtaatag acgaaggccg aggggtggtc ttccgtagtg
    10441 tagtaaatga tccggtcgtc ggcatcttgg gcgtccgatc cgccaacaaa gggccctgag
    10501 ccgcgaacga acgcactttc gggcagcacc ccctccggga tctcggtata ggcgtcgcga
    10561 tcgagataga tggtgtcgtc ggcgctggtg aaatcccgta ttttcttgtc gaagccaaga
    10621 agctcgaagt agaaaccgtc ctcgccggcg ccgccggtga gcgtgtcgaa gccccgaccg
    10681 ccatagaggc tgtcgttgcc gccgcggccg ttgagtacgt cgctgccccc catccccatg
    10741 agcacgttgt cggcggaatt gccgacgatg cggttatcgg ttctcccgcc cgtgccgtcg
    10801 atcaccgccg tgccgagcag cacgagatcc tcgacgtaaa tgtcctcgat gaccgtctgg
    10861 tcgaacacgc cgacgttgaa gctgacagtg ctgcgcaccg tatccaggcc ctggcccagc
    10921 tcctcgatga aatcgttcac gtcatcgaca taataagtgt cgtcgccgtg cccgccgctc
    10981 atgaagtcgg cgccgccgcg gccattgagg acattgtcga catcctggcc ggcctcgacc
    11041 gtcaggcggt tgtcgaggtc gttgccgtcg ccgcgcaccg cttcgccacg aagctcgaga
    11101 ttctcgatgt tgcgccggag ggtgaaggag atggtggcga aaaccgcgtc gctctcgccc
    11161 tcgccgagcc gctcgaccac cttgtccagc tcggaatcga cccaataagt atcgctgccg
    11221 gtccggccga tcaggagatc atcttcggtg ccgccgtcga tcgtgtcgtt gccggcccgg
    11281 ccgcggatcg tgtcgtcccc gccgaggccg aaaatgtcgt cctgaagccg cgtcccgttc
    11341 agtgtttcgc ccgcggcagt gcccgttatc gtcgccattc accgattcct tctgataaag
    11401 ccgtttgcgg cttgctgctc agattaaacc atgtttccca gcaaattacc agcctcggcc
    11461 gcaattgcca gcctcggccg cccgccgccc gcgaaatcca gttgacaacg tggccctccc
    11521 tgtcttggtt gctctgggat ctcggaccag ggatcatccg aaacggacca gtggggtggg
    11581 gacatgattg acgtagttga tctggcgagt ctggacggca agccggtgag cgcgggcgac
    11641 ttcatcaccg tcggcggctt ctcggggggg gtgttcgaag cacgctccag tagctccgta
    11701 ctggaggctt cgaaagtcac attacgcttt ctgcctcgcc cgttcaccgc aaatctgacg
    11761 gtttgggacc atgtctggga tgcagagacg gagaaaggcg ccaccaatcc gggcggaaag
    11821 cacggcgaca cgatcacgat cgacggacat caaatcacat tggtcagctc ggctccgacc
    11881 gggcagcagg tccagatcgg cataggcgcg accgacgccc tcaaagccgc cgacctcgcc
    11941 gagaagatca tgtcctacgt aaacgtcaac tcggtcctca taggggtgcg gatgcgcggc
    12001 actaccctgc tgcgctgcag cgcgaacgat tggggtcagc gctttgtcgg gggcgtcgcg
    12061 agccccgtgg ccgtgtccgt ttcctcaacc gacgcccacc caccgttcac gtgggacgta
    12121 acccacttgt ctgatccttc ggagggcagc tcggtgcggt tcggcgatca gccgaccgac
    12181 gttcttacat tcaaagcgcc tccggtcatc ggcgcaaacc gggagatagc gatcggggct
    12241 actccctcgg cgaccgcgca gaatttcaag aagtacatca atgagaggag cgagacgctg
    12301 gacctcaccg cttccggcga ggttaccgat gcggccaagg ggatggacat caccctcact
    12361 ccaagctcgt tcgcggggct cgcccggctt cgcaatttcg tcaaaggcgg cctgaacgtc
    12421 accaacgccg gggtggtgac gccaaagcca ccggagtgcg acctcttcgt ggtgcaggcg
    12481 atgggcgcgg cggcggcgga caagctgggc gcgatctacc gcccttccac ggcatcgccg
    12541 acggtggtca acgtccgcgt gagcgtggag cgggacggga cgctgcatcc ctccttctgg
    12601 gacttgaagg gcgaacgcgg ccacgacggc gactacatcc agccgcgcat gaacgccatg
    12661 tttgacgccg cgcatttcct cggctgcagg atcgagcttc gcgccggcgc caattacgac
    12721 ctcacccgcg gcgacggcta tacaaatcgc acgcagatgg acgccatccg gtttcgcaac
    12781 ataagcctgt acggaaacgg cgccaccata agcaaccgca acgacatcaa gcgttcagcc
    12841 accccgtcga cgcccggtga ggcaggcgag caaaacagca tcttctattt cggcgtcggc
    12901 catccggcgt tcgattccga cgttcctttc gcgcggatcg tcgcggctgc gaaaggcgac
    12961 acctatgtcg aactcgtact gctgaatacg ggatcctatt tccgcgtcgg cgatgtgatt
    13021 ccgatcatga cggtggcggg ccgatcggcc agaaccgttt ggcatcccgt cgcacgcgaa
    13081 ttctacaaga tcgtccatat cgacggaaag atcctgcatc tcgatcgtgg gctcgcctcc
    13141 gattatccgc tgaactcgcg ggtcgcgctc tggatgggcc attatgacag caccatcagg
    13201 caggacatct tcatcgcgga caatccacgc gtgcgcgatc tgtcgctgta tagcccctat
    13261 ggcggcgcga tgacccgcag tcaggcctat aatgtcgact tcgagttcga ccttctggaa
    13321 gcctattatg gcgtgttcat caacgccatg aacggtcacg tcaaggtgaa gaaggtgcgc
    13381 gccaagaaga aggcgtgcga cctcgcccac aactcccagg gcgtcaccgt cgacatggac
    13441 gaggtggaat ggtggcccaa cgatacgagc gacgacgcgc cgatgatgca cttcgccgag
    13501 aacagccggg attttgtgat cacctgcaag ctgctgcgct gcatgaattc ggctgacgag
    13561 ccggacttcc ccggtccgca gatcatcaac ggaaccgacg gcgcgcgcgg gataatcaag
    13621 ctgggtatgt cgattgtcga gaagatgctg aacggcgtga cggaaaaagg cgactgggcg
    13681 accgccaccg cctaccagat aaacgacatc gtcgctgtcg gatccaaaaa gtacgtcgcc
    13741 cgaaaggcgc atacatcctc cgcggatttc gacgccaagg aagagctcaa ttgggctccg
    13801 caggtccggc ggcagttctt caccctgctc cacaaggact cgctgcgcgg cgaggctccg
    13861 caccacttca ccgacctgga cctcgatctt gggcaggtat tttacggcga tgcgcccgga
    13921 gcgctcgtcc gcatccacaa gaacacggtg aatccgctgc tgggcccgaa gcggatccga
    13981 gtatcagggg ggcggttcaa cggcgcggaa ccgttggtta acctgggtgc gcacgttgag
    14041 ggcgatgact gctcgatagc ggagggagtc catattcccg gaaggctcag ggtggatggg
    14101 aacggcggcg tctatggcgg ccacttcacc cagggctatt caggagaaag cgtcggcgcc
    14161 ccgaccaggg tggcgatcag ctccgacatg accccacagc tgacgaagcg tgtcgtcaga
    14221 tcgatcggca tcaacagcac cgatacggcc aacacctacg cgcccggtac tctcattctc
    14281 ccacccggca ctttgggcca ggatggggat gcgctcgaac tcacgatcgc ctatgaaacc
    14341 agcaccgggg cgggctcgaa gaacatccag ctgtttggcc aatcgaccgg cgctgcggtg
    14401 caactcgccg caatcctcct gccggccaac gcggtcgccc tgggcgaagc ggttttcagc
    14461 ctgcggcgtg ccgcgatcgg ggagcttttc gcctccgtct tcgatcggca aggaaatgcg
    14521 cccccgcaag cggggatgcg ggtcttgccg aacctgcccg tgctgaacgt gaacaccgat
    14581 ccgaacctgg acgtcgatac catcaccctg tcggtacgga tcttcatcgg cgccgccagc
    14641 ggcggcttca tcatcccgcg cgtgctgacc tggcggccgc tgctgtcgtg gcacgtctga
    14701 gcggcgccgg cggcctcaaa agggccgccg ctgcgccgcc accagcatcc gcagcagcat
    14761 cgctaccggc cgcggcacgc cgaccttgcc ttcgagccac aggctcacgg tcgagcggct
    14821 gacgccgatc gccccggcaa ggtcgttctg ggtgcggtag ccgagcgtcc gcatcgccga
    14881 gcggagctcg tcgggtgaca tggaggaaag caggggatcg tccacggacg gtcccctagc
    14941 gccaaccggg cgcgcgggga attgccgccg cttgcgcgcg tgcccggagc gagctcagat
    15001 cgcgtccgag ccgcgttcgc cggtgcggat gcggagcgcc tcctggacgt cgatgacgaa
    15061 gatcttgccg tcgccgatgc ggccggtgcg ggcggcggtg gcgatggctt cgacggcgcg
    15121 ttcggcgagg ccgtcttcga ccaccacttc cagtttcacc ttgggcagga agtcgacgac
    15181 atattcggcg ccgcggtaaa gctcggtgtg gcctttctgg cggccgaagc ccttggcctc
    15241 ggtgacggtg atgccggaga cgccgacctc gtgcagcgcc tccttgacgt cgtccagctt
    15301 gaacggcttg atgatcgcct cgatcttctt cacgcttgct cccctcgggc gccggtccgg
    15361 cccagcataa gcccggcgcg ggacagggca agccggcgtt gacaggcgcg ggcggcggcc
    15421 gcactgtccc ggcgtcgcgg cgcgcgaggg gcgcggcgga tcgggtggcg ttcgggatcg
    15481 gcaggcgcgg cgctccggaa ggggagaggc ctgatgggta tcgaggttct agacggcgtg
    15541 gtcgaaaccg cggcggtgaa gcggtcgagc ggaaagctca ccatatacga cagtttgacg
    15601 atacgcctga aggacggcgg ggagcggcgg ctcgaaaagg tcgcggcggc gccggccgtc
    15661 gccgcggcgc tgcagccggg cgtcgagggc cggttctacg gctataaggc gatcgaccat
    15721 cgtgggatga tcggcgtccg cacccgcgac ggccgcgccg ccttcgcgat cccgagcggc
    15781 aatgagcgga tcatgctgat gctggcgatc gccggcctcg cctggttcct gatcgccttc
    15841 ttcgcccgcg actcgatcac cttcctcggc ctgatcggcg gagtgttggg cgcgatcggc
    15901 tatgtccgct accgccagac gcggatcgcg ggccgggcgc tgtacgatgc cgacgccggc
    15961 tacgcctgag caagccgagc ctatccccag tggctgaagg cggggcgttt ggcgtgcttg
    16021 cggccgacca ccttcagcga caccatcgtc ttcaagtcgg cgagggtgcc gaggctggcg
    16081 ccgcaatcct cgcaatcgat ctcggtttcg tcgccgcctt cgagcgggat gctgaggctg
    16141 tcgccgcggc aacggtcgca gcggaggagg acggaaatgg tcatgcggaa gctcccggaa
    16201 ccgcgggcac aatgcagggc aagcgatatg gttgcgaggc gcgaagattt aaccgcctgg
    16261 cgccggcggc ggctttcccg gccggccgcg cttctgctag ctttgcccgg aaaaaagggg
    16321 attcggcatg gccaaggcga tcggcgcggc attgttgtgg ggggcgctgc tgctcggcgg
    16381 ctgcgtttcg atcggcggcc cccggctggg atcggagctg gtcggtcgca gcgcgcggct
    16441 ggtgccggcg cggggcgatg cctcgatgct ccatttcgcg cctgacggga cggtcgagtc
    16501 gaccttcggc cggcgcagcg ccagcggccg ctggtggatc aagaatcgac gcctctgctt
    16561 cctctgggcg ggcagcttcc gcgaatgctg gccctatggc gcgccgcttc agccgggccg
    16621 gccaaggtcg atccggagcg accgcggcaa tgtcgtgcac gtgaccctgc tctaggcggc
    16681 gctcaataag gcggccggtc gagccccttg gggctcttgg tgaagatctc gcagccgtct
    16741 tcggtgatgc cgatcgaatg ttcgaactgg gccgacagcg agcggtcgcg cgtcaccgcg
    16801 gtccagccgt cctcgagcac cttgcaggcc gccttgccga tattgatcat cggctcgacc
    16861 gtgaagaaca tgcccgggcg cagctccggc ccggtgccgg ggcggccgac atggaccacc
    16921 tccgggctgt cgtggaacag ctgcccgagc ccgtggccgc agaaatcgcg cacgacgccg
    16981 tagcggtggc gctcggcatg gctctggatg gcgtggctga tgtcgccgag atggttgccg
    17041 gggcgggcct gttcgagccc gagcatcagg cattcatagg tgacgtcgac cagccggcgc
    17101 gccttcaccg gaacgtcgcc gaccaggtac atgcggctgg aatcgccgtg ccagccaccc
    17161 aggatcggag tcacgtcgat gttgacgatg tcgccgtcgc gaagcacccg gtcgcccggt
    17221 atgccgtggc agacgacgtg gttgatcgag gtgcagctgc tgtgggtgta gccgcgatag
    17281 ccgagcgtgg cggcgaccgc gccggcgtcg cgcatcatcg ccgagacgat gtcgtcgagc
    17341 gcctgggtgg tgacgcccgg aacgacgtgc ggcaccagcc cgtcgaggat ctcggcggcg
    17401 agccggccgg cggcgcgcat gccggcaaag ccttccggcc cgtggagctt gatcgcgccg
    17461 ccgcgctcgg gcgcgggcgc gacgtcgtag cgctggatgg tgcgatattc ggtcatggcc
    17521 ggaatatagc ccgcgcgcgc gggtttgacg aggtgttgac aatgttctcg tctttgggtt
    17581 aagtcatagc ttaactcgat gtaagggaga gagtagcctt cgggcctgcg ccggatttat
    17641 ccggctcatc gaggagattg caaagccgct catgcggcga tggtcttctt gtgcccggag
    17701 gtcgagatgg agcgaggcga gcggttcgcg ttattcgttc aggagatgga ggaagcggcg
    17761 cccgccgcat cgttcgagga ggcgcggcaa ttgctggaag ccaggctcaa ccagattgaa
    17821 gacgcgcatt cgggcgtgcc ctattgtccg gagaactggc gcgacgatgg ccgcatgtat
    17881 cctccgcagg acgattccga acggcacagc ccgatcgcgg gggtgcgcgt gtttcggacc
    17941 cgcgggcacc gcgtctcgat tgccgccaat ggcgcgatcc gcatcacgac ggcaggaacg
    18001 ctgcaggagc gcatcgtcct cgataaggcc ggaaaggatg gcgggtcctg tcccgccgcg
    18061 tgaccctccc atgaccgtct tcgatcagct tgtggacaga gtgcggcagc ggttcgatac
    18121 tgcgcaggac gtgtccgtgt ccgtggaaca gcccgcgagg cccgacggca cctattttct
    18181 taccatcgct tgcgacgact atgccgtcga agtggaatgg cagcgcaatc gcggcttcgg
    18241 catcgcggcc ggacgcgacc tggtgttcgg aagcggagtg gatgaggttc acggctcggc
    18301 cgcggccgcg ttcgaccgca tcgagcatct tctgcagacg cgtgaatcca catccggcga
    18361 ggcgagcgtc aatctcatcg acctccggaa gcttcgtgga ttgcttcaga aagacgtcgc
    18421 ggagcagctc ggcatcagca agagcggagt cgcgcagatg gagcgcgcgg agtcgctgac
    18481 cacgatgcaa atcgatactc tgcggaagct gatcgccact ctcggtggcg aactcgtgct
    18541 gtccgcgcgc tttccgggag gacgcgaacg ccggatcgcc gtggactgac cggttccttg
    18601 cccgcttcgc aaaagcctga gcagtcgcta gggcgatgaa atggcgagcg agcggatctg
    18661 gacggcgggg ttgttgatca tcggcgacga gatcttgtca ggccgcaccg aggatcggaa
    18721 tatcgcgccg atcgcgcgct ggctgaacct ccaggggatc aggctcggcg aggtgcgggt
    18781 ggtgcccgac gtagaggcgc ggatcgtcgc ggcggtgaat gcgctgagga ccgcgcacga
    18841 ttatctgttc acgaccggcg gcatcgggcc gacccatgac gacatcaccg tcgacgcgat
    18901 cgcggcggcg tgcggggtcg gggtcgaagt gcaccccaag gcgcgggcgg cgctggaggc
    18961 ttattatgcg agccgcggcg gcttatccga ggcgcggctg cggatggcgc gggtgccggc
    19021 aggggccgat ctgatcgaga acccgatgtc gggcgcgccg ggaatccggt tcggcaacat
    19081 cttcatcctg gcgggcgtgc cgcatatcgc cgctttgatg ctggaagcgc tcagcggccg
    19141 catcgaaggg gggaggccgc tcctatcccg gacgatcggc tgctgggtcc cggaaagcga
    19201 gatcgccgag accttgcgcg acaccgagcg ggcccatgaa ggctgccaga tcggcagcta
    19261 tcccttcttc cgcgagggcc gcgtgggcgc caatttcgtc atccgcagca ccgaggccgc
    19321 ggcgctcgac gcctgcgtgg aggatctgat cggccggctg gaagcggccg gctacgccgt
    19381 cgcccgcgac gggatctagc ggggcggcgc ttccgctagt gcggtcccgg tggcgctcgg
    19441 cagcgccggt caatggtcgt tatgccgcgg cggatgggct tcgagcgagg cggcgagggc
    19501 ggcttcctcg gcgcccggcc gccgccgcag cacccggcgc acggccaccg ccatcagcgc
    19561 gaagccgagc agcgacccgg ccagcaccgc cgccgccgcg ccgatggtgc ccgcttgcgg
    19621 gatcaggacc agcatcagtc cgatcagcgc ggcggcggcg atcagctgca cccggaacga
    19681 gcggccggcc tggccgctgg cgatcagcgc cggctcgaag gcgacaccga tcaggtcgac
    19741 cacggcggcg gcgccgagca gcagcagcag cgggtaggcg gcggcgaatt ccggcccggc
    19801 gaccagctcg agcagcggcc gtccggccag caggagcagg agcagcagca ggccgccgcc
    19861 gagcgcggcg gtgcggacga tgcgcgcgat catcggcccg aactcggcgg acgagccggc
    19921 ggcggtgcgg attcgcatca gctccggaaa caaagcgcgg gcgaacaatt gcgacacccg
    19981 cgccatcgcc cggccgagct ggtgggcgag ccggtaggcg ccggccgcgg ccgggctggc
    20041 gaagaagccg accagcagca ccggcagctg gcgggtggcg agggtcagcg tctgcgcgct
    20101 gttggtgatc gccgcgaaat ggaggatgcc gggattgtcc gcgcggaggg cgggcaggga
    20161 caaaggcgcc cggcgccagg gcagcccgcg cgcgacccgc gccgccgaca cccagaaagc
    20221 gatgctggag gcgagctcgg cggccgccca ggcgatgagg aagccgcgca cgctggggcc
    20281 ggcggcgagg accaggcccg cgcccgccag ccgcaccacc ggcagcgcgg tatcggcgaa
    20341 ggcggcggcg gcgaagcggt cgtggaggcg aaggatgccg gtcggggtcg agcgcatcga
    20401 cagcaaagcc gcgcaacaga acaggaaggt gtcgcgccgc atgtccggcg accagccgaa
    20461 ggcgggcgcg agcgcggcga ccgcggccat tgcgatcagg ctgccgagga gggcgccggc
    20521 ggcgtcgagc gcgagcgcgc ccttgagcag ccgggcgagc cgatcgggcc ggccggcgtg
    20581 cagatgctcg gtgccgtagc ggacgatgat ctgccaggtc tggaagctga tgatggtgac
    20641 gatggccccg ccggtgccgg tgacgagcgc gaactggccg aagccggcgg cgcccagcga
    20701 acgggtgacg atggcgatgt agaacaggct gaggatggcg ttgacgccgc ggcttccgag
    20761 cagccagcgc aaattggcga gcgccccgcc gaggctgccg gagcgcagcg gctggggcgc
    20821 ctccgccatc gccgggctaa gcgcgcggcg cgcgcggccg cggcggatgt ttcagatggt
    20881 gggcgataag ctcgccgaag cgctggtggc caacgcccct gcgcccggcg cgctgcgcga
    20941 cgatcatgca cggcttgcca ttgccttcga tcagcagcgg cccttcggcg gtgagcgcga
    21001 cgtcccagcc gaccagattg taatcggcga agccgccccg atgcgccttc agcaccagtg
    21061 ccttggcctc ttcccagaat ggcaggcggc gcccggcgat ggccgcgccc gaggccggat
    21121 gctcggttac gtcgccgacc cagcggccgc ggcaggcggg gccgagcaat ccggtggcga
    21181 ggtcgatcgg cgccatcagg ccgcccgact tgatattgtc gacctgcgaa ttcgggtccg
    21241 acgggaagcg gagcacagag gtgacgatct ccggctcgcc gcgctcgttg atcatggtgc
    21301 tgacgcgcgc ggtggagagg gcgctcagcg agaggtcgcg gagatccgga tggaccgaga
    21361 ccttgggctg gatcagccag gcgcccggga ggcggccgaa ccgctcgcgg acgaagcggg
    21421 cgaaggcggc ggcatcgccg tcggcggcgg gatagtcgaa cagctggaag ccgctgccgc
    21481 cttcgccgtc gaccggcttc agcgcgaggg cggcgaccgt cggcgcctcg cgcaccgcga
    21541 cggcgccgtc cacgatggtg gccagcagcg gcgggtgcgg cagtccaagc tcggcgcagc
    21601 gcgcgtagaa caggcgcttg tcggcgagca cgcagccggc cgcccaattc ttggggttga
    21661 tgcgcttgct gacgtccgca tgctcgaagc ggcggataaa gccgtccaga tcggcttcgc
    21721 tctcgacccg gaacagggcg atgtcgttgg ggtcggcgaa gcggcgatgg gcgatgcgcg
    21781 ccgccgtgcg cgcccagtcg cggccgagcc cgtatttgcg ggcgatgctg cgcgcccggt
    21841 acgggaccca cagatggaag acgccgccgg ccaaagcgtc gagcgccgcc cgcaccggcg
    21901 agcgctgcgc ccggcgcgtg cgataatgtt cgtcgaggaa gcccgccgac gtcggccgca
    21961 gcacgcccgg atagagcgcg aagacctttt tgagccggta gtaaaggtgt tgcgctcggg
    22021 tcattttatc caggcgtgcg gccgctgggc cgggcgggcc gcgcttgaga cgttggctgc
    22081 cggttaagcg agcgggggtg agggatcaag ggcgtcgcgc cgtgcaccgg ggcagtcagc
    22141 ggctgaaggt cagcaccggg gcgtcgcgga agcgggcgtc ggggagccgg gccagatggt
    22201 ggcggtagag cgcgccgagc cgggtggcgg cgatgccgcg gccggagacg gtctggagga
    22261 gattgccgcc ggggttccaa ttgccctcga ggatgaccgg cccgtcctcg gccaaagcga
    22321 cgtcccagcc gaccagcagg aagtcctcgc cgagcagcgc ccggtggccg agcaccgcct
    22381 gccgcttcgc ctcgtcccag ccgggcagcg cgaagccggc gatgggggcg ccggtggaag
    22441 gatgcacgtc gtggctgccg atccgccagc ggctgctttt ggagcgggca ggcccggcca
    22501 cgccggtggc gagatcgacc ggcgcggcga ggccgccggc gctgaaattg tcgacgatga
    22561 cgctgccccg gccgagccgc agcgtggcgt cgaccagctc gatggcgccg aactcgtcga
    22621 ggcaggtcac cagccgcagc gtcggcagcg ctcccggtga cagcgcggcc agcgcttcgt
    22681 gggtgcgcag gcgctgctgg agcacgtcgc cggggccgag cgcgctttgc gcccaggcga
    22741 gcaggtctgc caccggctgc tcgcggccgc cgcgctccgc gacccagccg agcccgtgcc
    22801 ggcggagccc gaagatgccg cggccgcgcg agctgaagcc gggcttgaag atcaactcgc
    22861 cggccgcctc gatccaggcg ggcaggccgg cggcgagatc gccctgggcg agggtcggga
    22921 tgccggtcat cccggactgg cgcgcgatga aggcggcgaa gcgcgccttg tcgaacagga
    22981 tcgagtcgcg cttcgggaag gcgcgcgggt tgaggaggag gttgatgccc ttcttgtcgt
    23041 aattgccgat gagcgcgccg gcggccccgc cgaccccgct ctcgcgcgcc acccgaagct
    23101 cggtcggatg cacgttccac ttgagcgcat cggcgaggtc gcgcaccgcc cggcgaagcg
    23161 agccggcctg cccgaggccg gtcagcgcca gcgccgcctg cggcaggttc atggccagcg
    23221 ccgcgagccg ccgccgccat ggccagtggc tccacaggcg ccagtgataa aggtcgaccg
    23281 gatgcgcgga cccgcgccgc ccgcgcgcga tccgtgccgg cttggcgacg cggatgaccc
    23341 agctcaaggg cggctgcccg cgatttccgg ctcctcatcg ccgctccgcc accaatagct
    23401 cgcggcggcg gtcagcatcg ccatcgccag ccccagcgcc aaggagtgaa ccgcgccagc
    23461 agatccgccg tactggcgat gaagagcagc gcgaagccgc tgcaggcgag cagcgcatag
    23521 ccggcgatgg cgcgcggccc cgccgggacc agccggagct tcctcgccca gcgcagcgcg
    23581 ggccccgcga gcaggaggga gcggcccgaa caggaagacg gcaaggagca tcgcgccggt
    23641 ata
//
LOCUS       DASVRK010000132        12083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_272920, whole genome shotgun sequence.
ACCESSION   DASVRK010000132 DASVRK010000000
VERSION     DASVRK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272920"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF589_04990"
     CDS             <1..362
                     /locus_tag="VF589_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8256966.1"
                     /translation="FLAQQFDAQHRHYQGHYPAAEWLMIERGGERIGRLYLVEWPSQF
                     RVIDITLAEGCRGRGIGRAILEDVQSMAAAAGKRVSIHVEKMNPAMKLYLRLGFERSE
                     DKGVYDLMEWRPGGPVS"
     gene            complement(383..682)
                     /locus_tag="VF589_04995"
     CDS             complement(383..682)
                     /locus_tag="VF589_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256967.1"
                     /translation="MSDQDQARDPGIEDFAANVGERYEVAGPDGNLPLTLEEARPLGH
                     SARPGGAFALTFLGPADPVLPQAIYRLSRGDEADDIFIVPITQDAFGTRYEAIFT"
     gene            complement(679..1164)
                     /locus_tag="VF589_05000"
     CDS             complement(679..1164)
                     /locus_tag="VF589_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX8256968.1"
                     /translation="MRPALAASTLVIGALLYGPAAAQKERPTPYWASIAANEALMRTG
                     PGKNYPGIWLYRRADLPIKVLEVYPSWRKVQDPGGTTGWMLVNLLSDTRTALVTGGEA
                     RPLYEAPQDAARVKFRAQPGVVGRISRCASGWCRFDVQGRDGYIRADHIWGVDPNETV
                     E"
     gene            1274..2272
                     /locus_tag="VF589_05005"
     CDS             1274..2272
                     /locus_tag="VF589_05005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX8256969.1"
                     /translation="MPRRAPPPRPKLIVTRRLPASVEARMAELFEAEFNADDHAFTEA
                     ELIAASARADVLVPTVTDAIDAAVIEGAGERLQLIANFGVGTDHIDLKAARARDIVVT
                     NTPGVLTEDTADLVMALILAVPRRLAEGEKLLRAGEWRGWSPTGLMGHSVTGKALAIF
                     GMGRIGRAVAKRARACGLSIHYHNRERLPERVERELGAVFWPDLDAMLAAADVLTVNA
                     PHTAETHHVIDARRLSLMKRDAYLINAARGAIVDQEALIEALEAGVISGAGLDVFPHE
                     PEVDPRLLRLPNLVLLPHMGSATFEGRTAMGEKVIANIRAWADGHRPPDQVLEGWV"
     gene            complement(2439..3845)
                     /locus_tag="VF589_05010"
     CDS             complement(2439..3845)
                     /locus_tag="VF589_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8256970.1"
                     /translation="MFKNIRPIRYAGREVLPLVEGGKGVSATNHASSGAWAAAGGIGT
                     VSAVNADSYDPEGRIVPQVYNALTRRARHEELVQYAIDGAVAQVERAYDIAGGQGAIN
                     INVLWEMGGAQRVLHGVLERTRGLVAGVTCGAGMPYKLSEISASYGVNYLPIISSARA
                     FSALWKRAYSKAAEWLGAVVYEDPWLAGGHNGLSNAEDPLAPQDPYPRVKALRETMRA
                     GGISDEVPIVMAGGVWNLKEWEHWIDNPELGQIVFQFGTRPLLTKESPIPEGWKARLM
                     TLEEGDILLHRFSPTGFYSSAVRNPFLRSLEARSHRQIAFTKEELGDHAFQLDVGVGT
                     RKNYWVTRGDLLHAREWYAQGFTEALKTPDETLIFVTPEEKQMIRKDQADCMGCLSQC
                     AFSSWADNEKNATGRLADPRSFCIQKTLQDIAHGGPVDNNLMFAGHAAFRFKKDPFYS
                     NGFVPTVKQLVERIQTGD"
     gene            complement(3905..5041)
                     /locus_tag="VF589_05015"
     CDS             complement(3905..5041)
                     /locus_tag="VF589_05015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8256971.1"
                     /translation="MPVRILHAHSTFSLGGKEARAVRLMNAFGAAAEHTILSGMPRQL
                     SARAAIAPGIKVEFPSDAPPLTGRPALSRLFRLARYMRRFDLVLTYNWGAFDAVMAKY
                     LFRGPPLVHHEDGFNEDEAAGLKTRRNLYRRLGLSAAHRLVVPSGELERVARTIWAQP
                     PHRVERIPNGIPVANFAAPPPDDAIKCVPRRPGELVIGTLAGLRAVKNLPRLVRAFAA
                     MKAGKPLLVIVGEGPECERIVAEAHRLKVADRVHLPGFLADPASYIGRFDIFALSSES
                     EQFPISLVEAMAAGLPAVATRVGDIAQMVSPDNRPLIVEPADEAAFAAALDTLAERPD
                     LRRSIGRANREKAAAEYDERAMIARYASMYSEALGRPGALTPAP"
     gene            5153..6499
                     /locus_tag="VF589_05020"
     CDS             5153..6499
                     /locus_tag="VF589_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX8256972.1"
                     /translation="MKAGTLLMLACACSAVPAAALPADNQVEARAPALPPILSLRERA
                     KLEDRWLKRRLETLVPALMREHGFDMWVMVAREYLEDPVVATMLDAESMHARRRTILI
                     FFDPGGGKPVERLTVSRYGLGDLFEGAWDPAKQPYQWKRLGELIAERDPKRIAINSSA
                     LTAFADGLTLSQHQGLMAALPERLRGRVTPTDALAVGWLETRIPEEIAAYPGIVRIAH
                     AIIGEGFSGKVITPGKTKAADVVWWFRERISGLGLGAWFQPSVGIWRRGEKAMIEGDA
                     VIRAGDMLWTDFGITYLGLNTDTQHLGYVLRPGEGDAPAGLKRGLASANRVQDALTAA
                     FKTGRTGNQMLALARAEALRQGLKPSIYSHPLGYHGHGAGSSIGFWDNQGPSPQGEHP
                     LRPHTAWSIELSARANVPEWDGQEVEFRSEEDAYFDGERVRYLDGRQTEFHLIGGR"
     gene            complement(6503..7363)
                     /locus_tag="VF589_05025"
     CDS             complement(6503..7363)
                     /locus_tag="VF589_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8256973.1"
                     /translation="MVAWSDGYWWSNDGLRLHYRDFAGDPSRPPILCIPGLTRNARDF
                     EAVAARLAGDWRVICVELRGRGESAYAKDPMTYVPLTYLQDIEALIRELKLDRLILFG
                     TSLGGIITMLLAMSGAGRIAAAVLNDIGPDLDPRGLDRIRAYVGRSQSWPTWLHAARF
                     FAEAQRDRYPDWALDQWLVFAKRLCRLTPNGRIALDYDMRIAEPLKLPGGEAGFDMWR
                     AFEGLRGIPSLVVRGGLSDLLAPATVERMQAENEAMEAVTVERSGHAPTLEEPEAVAA
                     IDRLLARLSA"
     gene            7400..8812
                     /locus_tag="VF589_05030"
     CDS             7400..8812
                     /locus_tag="VF589_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX8256974.1"
                     /translation="MSRSPQPAPYRAETAILSLGEAFYDPVEAADFPRTILRFRNDRW
                     APSVGLDGLGDDQWLRHFGRFEPLPDNLPQPLALCYHGHQFRAYNPDIGDGRGFLFAQ
                     LRDHGGRLLDLGTKGSGQTPYSRFGDGRLTLKGGVREILATEMLEALGVETSKTFSLI
                     ETGEALDRNDEPSPTRSAVLVRLQHSHIRIGTFQRLAFFGEADNIRRLTRYCLDHYYG
                     GDGGAADDEAAAAALLGHVARTAARLAASYIAAGFVHGVLNSDNIAITAQSFDYGPWR
                     WTPYWDGAFTAAYFDRTGLYAFGRQPEAIHWDVVQLAASLSLIAAAERLTAELDAYAP
                     TFQQALREAICARLGVVARGEDEDNALLAAIESGLAEKNVEIDRFFFDWRGGRRRGPS
                     PADERYDTEPFAEFRRLIDSYQPTAAPDHDYWADAAPCSMHIDEVEAIWSRIDQADDW
                     TALYDKVAAIRRMGEAMGGG"
     gene            8903..10045
                     /locus_tag="VF589_05035"
     CDS             8903..10045
                     /locus_tag="VF589_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256975.1"
                     /translation="MADIPFKSFYMAGFECSSHRRRGDGVRLDLIASTGHDRLVREDY
                     RACAQHGLLTLRDGLRWHLIEREPGRYEWSSWLPMLEAAAESGVQVMWDLFHYGSPDH
                     LDQGSDEFIRSYARFAAAAVRLHREVTGTAAIVCPVNEMSFLAWAARNGVFPPIGPDE
                     DGWLSRHLVRAAVAGMRAMREVDPDCRFIWAEPLINVLPLTPADAEAAEAKRLSQYHI
                     YDMLSGRAAPELGGSPDWLDAVGLNFYSDNQWYLDGSTIPLGRHDYRPLADMLAETYA
                     RYRRPLLITETGGEGSARPAWFHYVCDEVREAMRQGVPVAGVCLYPIVSFPGWDDERH
                     RQFGLFSVPRPQGRRDAYEPLAAELYRQQELLEGERRHEVRPAAAR"
     gene            10296..>12083
                     /locus_tag="VF589_05040"
     CDS             10296..>12083
                     /locus_tag="VF589_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256976.1"
                     /translation="MLSFASGHHAHSPRASRASRKIRIGLYLACTALSAPLQAGVPTM
                     AAAASPQPAPEAREEPFQDPAIVAAAADEGVVVVSRDDVYILEQRVVTSGDDSPAIFG
                     RSTGGGVTIINGEAETAGDDSVGIFAEAEGEGRNADVNAGTVITQGDNSGGIMVRAEG
                     GYVQDSPYGPLAYGNAIVTSQDVRTSGANSIGVAAQSIGGAALVTSNQVATQGDGSIG
                     IQAIGDAVVQVTSESVTTAGVGATGINAQSSEGGVTVQSGSVATTDAGAAGIVAEGYA
                     DVVVISDSVSTAGDPVGKETPAAVGILASSEAGNVVVESGAVSTQGRGATGIQANAYG
                     GVTITSASVTALGDGAKGIDVSNVTGAVNIDSGIVDVSGDLAHGINVLSEQGRVNITS
                     AAVTTRGDFSSGIKVVPGGSVTIDSGTVDTTGDYAMGIEIFSTGGDVTVASGAITTDG
                     TRSNGISAVALTSGGDVDIEADSVATGGFSSDAIYASAGSNLTVKAGSLSTRGEESTG
                     VFANAYDSVGITVESIATQGALSAGVSAQSEGGSVAVEVGSVTTAGDSSVGIYGGALE
                     DVTIAAGSVVTTGDRLVRTDPDTGETIYFD"
BASE COUNT         1921 a         4018 c         4266 g         1878 t
ORIGIN      
        1 cgttcctcgc ccagcaattc gacgcgcagc atcgccacta tcagggtcat tatccggcgg
       61 ctgaatggct gatgatcgag cgcggcggcg agcggatcgg gcggctctac ctcgtcgagt
      121 ggccgagcca gttccgggtc atcgacatca ccctggccga aggctgccgc ggccgcggca
      181 tcggccgggc gatcctcgag gacgtgcagt cgatggccgc cgcggcgggc aagcgggtgt
      241 cgatccacgt cgagaagatg aacccggcga tgaagctata cctccggctc ggcttcgagc
      301 gcagcgagga caagggagtc tacgacctga tggagtggcg gccgggcggg ccggtcagtt
      361 gaggccggtc agttgaagcc ggtcaggtga agatcgcctc gtagcgggtg ccgaatgcat
      421 cctgcgtgat cggaacgatg aagatgtcgt cggcttcgtc gccgcggctc agccggtaaa
      481 tggcttgcgg cagcaccgga tcggcagggc cgaggaacgt gagggcgaag gcgccgccgg
      541 ggcgggcgga atggccgagc ggccgcgcct cctccaaggt cagcggcaaa ttgccgtcgg
      601 ggcccgccac ctcgtagcgc tcgccgacgt tggccgcgaa atcctcgatg ccgggatcgc
      661 gggcctgatc ctggtcgctc actccacggt ctcgttcggg tcgacgcccc agatatgatc
      721 ggcgcggata tagccgtcgc ggccctggac gtcgaaccgg caccagccgg aggcgcagcg
      781 cgagatgcgg ccgacgacgc ccggctgcgc ccggaacttt acccgggcgg cgtcctgagg
      841 cgcttcgtac agcggccttg cctcgccgcc ggtgaccaac gcggtgcggg tgtcgctgag
      901 gaggttgacc agcatccagc cggtcgtgcc gccgggatcc tgcaccttgc gccagctcgg
      961 atagacttcg agcaccttga tcggcaggtc cgcgcgccga tagagccaga tgcccggata
     1021 attcttgcca gggccggtgc gcatcaaagc ttcgttggcg gcgatcgaag cccaataagg
     1081 cgtcggccgc tccttctgcg cagcggcggg tccgtagagc agcgccccga tcaccaatgt
     1141 tgaagccgcc aaggctggcc gcatctgaga gtctcctgtt gccgcccctc ccccacctct
     1201 aaccggcggc gcggcccggc ttcaaccggc atcttgcttg acgcccgata tcgcggcggc
     1261 ctagcgcgcg gcgatgcccc gcagagcgcc cccgccccgg ccgaagctga tcgtgacgcg
     1321 gcggctgccg gcttcggtcg aggcgcggat ggccgagttg ttcgaggcgg agttcaacgc
     1381 cgacgaccat gcattcaccg aagcggagct gatcgccgcc tcggcccgcg ccgacgtgct
     1441 ggtgccgacc gtcaccgatg cgatcgacgc tgcggtgatc gagggcgcgg gcgagcggct
     1501 ccagctgatc gccaatttcg gggtcggcac cgaccatatc gatctcaagg cggcgcgggc
     1561 gcgcgatatc gtcgtcacca acacgccggg cgtgctcacc gaggatacgg cggacctggt
     1621 gatggcgctg atcctggcgg tgccgcggcg gctcgccgag ggtgagaaat tgctccgcgc
     1681 cggcgagtgg cgcggctggt cgccgaccgg cctgatgggg cacagcgtca ccggcaaggc
     1741 gcttgccata ttcggcatgg gccggatcgg ccgcgcggtc gccaagcggg cgcgggcctg
     1801 cggcctttcg atccactatc ataatcgcga gcggctgccg gagcgggtcg agcgcgagct
     1861 cggcgcggtt ttctggccgg acctcgacgc gatgctggcg gccgcggacg tgctgacggt
     1921 gaacgcgccg cacacggccg agacgcacca cgtcatcgac gcgcggcggc tgtcgctgat
     1981 gaagcgcgac gcctacctca tcaacgcggc gcgcggcgcg atcgtcgacc aggaggcgct
     2041 gatcgaggcg ctggaggcgg gggtgatttc gggcgccggg ctcgacgtgt tcccgcacga
     2101 gcccgaggtc gatccgcggc tgctccgcct ccccaacctc gtgctgcttc cgcacatggg
     2161 ctcggcgacg ttcgaagggc ggaccgcgat gggcgaaaag gtgatcgcca acattcgagc
     2221 ctgggccgat gggcaccggc ccccggacca ggtgctggag ggttgggtgt agctcaaccc
     2281 ttctcccgct cgcgggagaa ggaggggccc aaagcgccag ctttgggagg atgagggtct
     2341 tttttgggac gctgcgcggt gctcggacgg tagttcaaga aggaagaaga agaccctcac
     2401 gctgccctct cccgcgagcg ggaaaggggg acgtcgcgct agtcgccggt ctggatgcgc
     2461 tcgacgagct gcttcaccgt gggcacgaag ccgttcgagt agaacgggtc cttcttgaag
     2521 cggaaggcgg cgtggccggc gaacatgagg ttgttgtcga ccgggccgcc atgggcgatg
     2581 tcctggagcg tcttctggat gcagaagctg cgcgggtcgg cgaggcggcc ggtggcgttc
     2641 ttctcattgt cggcccagga ggaaaaggcg cactggctga ggcagcccat gcaatccgcc
     2701 tgatccttgc ggatcatctg cttttcctcg ggcgtgacga agatcaacgt ctcgtcgggc
     2761 gtcttgagcg cctcggtgaa gccctgcgca taccattcac gagcgtggag caggtcgccg
     2821 cgcgtgaccc agtaattctt gcgggtgccg acgccgacgt cgagctggaa ggcatgatcg
     2881 ccaagctcct ccttggtgaa ggcgatctgg cggtgcgagc gcgcttcgag gctgcgcagg
     2941 aaggggttgc ggaccgccga ggaatagaag ccggtcggcg agaagcggtg gagcaggatg
     3001 tcgccctcct cgagcgtcat cagccgcgcc ttccaccctt cggggatcgg gctttccttg
     3061 gtcagcaagg ggcgggtgcc gaactggaag acgatctggc cgagctcagg attgtcgatc
     3121 caatgctccc attccttgag gttccagacg ccgccggcca tcacgatcgg cacctcgtcc
     3181 gaaatgccgc cggcgcgcat tgtctcgcgc aaagccttga cgcggggata gggatcctgg
     3241 ggggcgagcg ggtcctcggc attggacagg ccgttatggc cgccggccag ccagggatcc
     3301 tcgtagacca ccgcccccag ccattcggcg gcctttgaat aagcccgctt ccagagcgcg
     3361 ctgaacgcgc gggcggagct gatgatgggc aggtagttga cgccgtagga ggccgagatc
     3421 tcggacaatt tatagggcat gccggcgccg caggtgacgc cggcgacgag gccgcgggtg
     3481 cgttcgagca cgccgtggag gacgcgctgg gcgccgccca tctcccacag gacgttgatg
     3541 ttgatcgcgc cctgcccgcc ggcaatgtca taggcgcgct cgacctgggc gacggcgccg
     3601 tcgatcgcgt actggaccag ctcttcgtgg cgggcgcggc gggtcagcgc attatagacc
     3661 tgggggacga tccggccttc gggatcgtag ctgtcggcat tgaccgccga cacggtgccg
     3721 atgccgccgg cggcggccca ggcgcccgag ctcgcatggt tggtggctga gactcccttg
     3781 ccgccctcga cgagcggaag cacttcgcga ccggcatagc ggatcggccg gatattcttg
     3841 aacatcattc ctccaggcga gattggcagc gcctatagcg cctaatatca attaggccaa
     3901 agcttcatgg cgcgggagtc aacgctccag ggcggccgag cgcttcactg tacatcgagg
     3961 cgtagcgggc gatcatcgcg cgctcgtcat actcggcggc ggccttttcg cggttggcgc
     4021 ggccgatcga gcggcgcagg tcgggccgct cggccagcgt gtcgagcgcg gcggcgaagg
     4081 cggcttcgtc ggcgggctcg acgatcagcg ggcgattgtc gggcgagacc atctgggcga
     4141 tgtcgccgac gcgggtggcg accgccggca gccccgccgc catcgcctcg accagcgaga
     4201 tcggaaattg ctcgctttcc gacgacagag cgaagatatc gaagcggccg atataggaag
     4261 cgggatcggc gaggaatccg ggcaaatgga cgcggtcggc gaccttcagg cggtgcgctt
     4321 cggcgacgat gcgttcgcat tcggggccct cgccgacgat gaccagcaag ggcttgccgg
     4381 ccttcatcgc cgcgaaggcg cggaccaggc ggggcaggtt cttgacggcg cgaagtcccg
     4441 ccaatgtgcc gatcaccagc tcccccggcc gcctcggcac gcattttatg gcgtcgtcgg
     4501 gcggcggcgc ggcgaagttg gcgacgggga tgccgttggg gatgcgctcg acccggtgcg
     4561 gcggctgcgc ccagatcgtg cgcgccaccc gctcgagctc gccggatggc accaccagcc
     4621 ggtgcgcggc ggaaaggccg aggcggcgat agaggttgcg gcgggtcttg aggcccgccg
     4681 cctcgtcctc gttgaagccg tcctcgtgat gaacgagcgg cggcccgcgg aacagatatt
     4741 tggccatcac cgcgtcgaac gcgccccaat tgtaggtgag gactaggtcg aaccgccgca
     4801 tgtagcgggc gaggcggaac aggcggctga gcgccggccg gccggtgagc ggcggcgcgt
     4861 cggacgggaa ttcgaccttg atgccgggcg caatggcggc ccgcgcggag agctggcgcg
     4921 gcatgccgct gaggatggta tgctcggcgg ccgcgccgaa cgcgttcatc agccgcaccg
     4981 cgcgcgcctc cttgccgccg agcgagaagg tggaatgggc gtggaggatg cgaaccggca
     5041 tgccgcctta tggcgcagcc gccgacggca aggaagccgt gagcaatacg gtcgacacgg
     5101 tcgcggagga aggcgatgta gggcatcggc tgagcggcga gaggaagcga cattgaaggc
     5161 tggaaccctg ttgatgctcg cctgtgcctg ttcggcggtc ccggcggcgg cgctgccggc
     5221 cgacaatcaa gtggaggcca gggcccctgc cctgccgccg atcctgtcgc tgcgcgagcg
     5281 ggcgaagctc gaggaccgct ggctgaagcg gcggcttgag accctggttc cggcgctgat
     5341 gcgcgagcat ggcttcgaca tgtgggtgat ggttgcccgc gaatatctcg aggacccggt
     5401 ggtcgccacg atgctcgatg ccgagagcat gcacgcccgc cgccgcacca tcctcatctt
     5461 cttcgatccc ggcggcggca agccggtcga gcggctgacg gtgagccgct acggcctcgg
     5521 cgacctgttc gagggcgcgt gggatccggc gaagcagccc taccagtgga agcgtttggg
     5581 cgagttgatc gccgagcgcg acccgaagcg gatcgcgatc aacagctcgg cgctgaccgc
     5641 ctttgccgat ggcctcaccc tcagccagca tcaggggctg atggcggcgc tgcctgagcg
     5701 gctgcgcggc cgggtgacgc cgaccgacgc gctcgcggtc gggtggctcg agacccgcat
     5761 tcctgaggag atagcggctt atccgggcat cgtccggatc gcccacgcga tcatcggcga
     5821 gggcttttcg ggcaaggtga tcacgcccgg caagaccaag gcggcggacg tggtctggtg
     5881 gttcagggag cggatctcgg gcctcggcct cggtgcctgg ttccagccat cggtggggat
     5941 ctggcggcgc ggcgagaaag cgatgattga gggcgacgcg gtgatccgcg ccggcgacat
     6001 gctgtggacc gacttcggca tcacctatct cgggctcaac accgacaccc agcatctcgg
     6061 ctatgtactg cgcccgggcg agggcgacgc tccggccggg ctgaagcgcg ggctggcctc
     6121 ggccaaccgg gtacaggacg cgctgaccgc cgcgttcaag accgggcgga ccggcaacca
     6181 gatgctcgcc ctcgcccgcg ccgaggcgct gcggcagggg ctcaagccca gcatctattc
     6241 gcatccgctc ggctatcacg gccatggcgc cggaagcagc atcggcttct gggacaatca
     6301 ggggccgagc ccgcagggcg agcatccgct gcggccccac accgcctggt cgatcgagct
     6361 cagcgcgcgg gcgaacgtgc ccgagtggga cggccaggag gtcgagttcc ggagcgagga
     6421 agacgcctat ttcgacgggg agcgggtgcg ctacctggac ggccgccaga ccgagttcca
     6481 cctgatcggc ggccgctagc gatcaggcgg agaggcgagc cagcaggcgg tcgatggcgg
     6541 cgacggcttc gggttcctcg agcgtggggg catggccgga gcgctcgacc gtgaccgcct
     6601 ccatcgcttc attctcggcc tgcatccgct cgaccgtggc gggtgcgagc agatcggaga
     6661 ggccgccgcg gacgactagc gagggtatcc ccctcagccc ctcgaacgcg cgccacatgt
     6721 cgaagccggc ctcgccgccg gggagcttga gcggctcagc gatgcgcatg tcataatcca
     6781 gcgcgatgcg gccattgggg gtgagccggc acagcctctt ggcgaagacc agccactggt
     6841 ccagcgccca gtcaggatag cggtcgcgtt gcgcctcggc gaagaagcgg gcggcgtgga
     6901 gccaggtcgg ccagctttgc gagcggccga cataggcgcg gatgcggtcg agcccgcgcg
     6961 ggtcgagatc gggcccgatg tcgttgagca cggcggcggc gatccgcccc gcgccgctca
     7021 tcgccaagag catggtgatg atcccgccga gcgaggtgcc gaacaggatc agccggtcga
     7081 gcttcaactc gcggatcaaa gcctcgatat cctggagata ggtcagcggc acataggtca
     7141 tcgggtcctt ggcataggcg ctctcgccgc ggccgcgcag ctcgacgcag atcacccgcc
     7201 agtcgccggc aagccgcgcc gcaaccgctt cgaagtcgcg ggcgttacgg gtgaggcccg
     7261 ggatgcacag gatcggcggg cgcgacggat cgccggcgaa atcgcgataa tggagccgga
     7321 gcccgtcgtt cgaccaccaa tagccatcgg accatgcgac catgcttcat cccccaatca
     7381 gccggaccca tagcatccta tgagccggtc cccgcaaccc gcgccctatc gcgctgaaac
     7441 ggctatcctt tcgctgggcg aggcgtttta cgatcccgtc gaggcggcgg actttccgcg
     7501 gacgattctc cgcttcagga acgaccgctg ggcgccgtcc gtcggcctcg acggcctcgg
     7561 cgacgaccag tggctgcggc atttcgggcg gttcgagccg ctcccggata atctgccgca
     7621 gccgctggcg ctgtgctatc acggccacca gttccgggcg tacaatcccg acattggcga
     7681 cgggcgcggc ttcctgttcg cgcagctgcg cgaccatggc gggcggctgc tcgacctcgg
     7741 caccaagggc tcgggccaga ccccctacag ccggttcggc gacggccggc tgacgctcaa
     7801 ggggggcgtt cgcgagatcc tcgccaccga gatgctggag gcgctgggcg tcgagacctc
     7861 caagaccttc tcgctgatcg aaaccggcga ggcgctggac cgcaacgacg agccctcccc
     7921 gacccgctcg gcggtgctgg tccggctcca gcacagccat atccgcatcg gcaccttcca
     7981 gcggctcgct ttcttcggcg aggccgacaa tatccgccgg ctcacccgtt attgcctcga
     8041 tcattattat ggcggggacg gcggcgcggc cgatgacgaa gcggcggcgg cggccttgct
     8101 cggccatgtc gcgcgcacgg cggcgcggct ggccgcctcc tatatcgcgg ccggcttcgt
     8161 ccacggcgtc ctcaacagcg acaatatcgc gatcaccgcg caaagcttcg attatgggcc
     8221 gtggcgctgg acgccttatt gggacggcgc cttcaccgcc gcttatttcg accgcaccgg
     8281 cctctacgcc ttcggccggc agcccgaggc gatccattgg gacgtggtcc agctcgccgc
     8341 ctcgctgagc ctgatcgccg cggccgagcg gctgacggcc gagctcgacg cttatgcgcc
     8401 caccttccag caggcgcttc gggaggcgat ctgcgcccgg ctcggcgtgg tcgcgcgcgg
     8461 cgaggacgag gataatgcat tgttggccgc gatcgagagc ggcctggccg agaagaatgt
     8521 cgaaatcgac cgcttcttct tcgactggcg cggcggccgc cgccgcggcc cctcccccgc
     8581 cgacgaacgc tacgataccg aacccttcgc cgagttccgg cggctcatcg atagctacca
     8641 gccgaccgcc gcgcccgatc acgattattg ggccgacgcg gcgccctgct cgatgcatat
     8701 cgatgaggtc gaggcgatct ggtcgcgcat cgaccaggcc gacgactgga cagcgctgta
     8761 cgacaaggtc gcggcgatac ggcgaatggg cgaggcgatg ggaggcggct gacggtcgat
     8821 caagtcgaac cttgattcag gttagcgatg ttttcgctcc gccccccgca acccatgcac
     8881 cgcccgggga ttggcagcgg tcatggccga catccccttc aagagcttct acatggcggg
     8941 cttcgaatgc tcgtcccacc gccgccgcgg cgacggcgtc cgtctcgatc tgatcgcgag
     9001 caccggtcac gaccggctcg tccgcgagga ttatcgggcc tgcgcccagc atgggctgct
     9061 caccctccgc gacggcctgc gctggcattt gatcgagcgc gagccgggcc gctacgagtg
     9121 gtcgagctgg ctgcccatgc tggaagcggc ggcggagagc ggcgtgcagg tgatgtggga
     9181 cctgttccac tatggctcgc ccgatcatct cgaccaggga agcgacgagt tcatccgttc
     9241 ctacgcgcgc tttgcggcgg cggcggtgcg gctccatcgc gaagtgaccg ggaccgccgc
     9301 gatcgtgtgt ccggtgaacg agatgtcctt cctcgcctgg gcggcgagga acggcgtgtt
     9361 ccccccgatc ggcccggacg aggacggctg gctgagccgc catttggtcc gggctgcggt
     9421 cgcgggcatg cgcgcgatgc gggaggtcga tcccgactgc cgcttcatct gggcggagcc
     9481 gctcattaac gtgcttcctt tgactcccgc cgatgccgag gccgccgagg ccaagcggct
     9541 cagccaatat catatttacg acatgctgag cggccgcgcc gcgcccgagc tcggcggttc
     9601 gccggactgg ctggatgcgg tcgggctcaa cttctactcg gacaatcaat ggtatctgga
     9661 cggctcgacg atcccgctcg gccgccacga ttaccgcccg ctcgccgaca tgctggcgga
     9721 gacctacgcg cgctaccggc gcccgctgct gatcacggaa accggcggcg aaggatcggc
     9781 ccgccccgcc tggttccatt acgtctgcga cgaggtccgc gaagcgatgc ggcagggcgt
     9841 gccggtggcg ggcgtttgcc tttatcccat cgtcagcttt cccggctggg acgacgagcg
     9901 ccaccggcag ttcggcctgt tcagcgtgcc ccgcccgcag ggcagacgcg acgcctatga
     9961 accgctggcc gcggagctct accgtcagca ggagctgctc gaaggcgagc gcagacatga
    10021 ggtccggccc gccgcggcac gctgactaac ccccgtcatt ccagcgacta ccccccgtca
    10081 ttccagcgca agctggaatc cgtttcctat tggcgcggcg caggaagaag aagtgagatt
    10141 ccagcttgcg ctggaatgac ggcggaaggg gcggatccag tcctccctgc ggcggcgggc
    10201 gcgttcgccg tcaccgtgca aattgcggtg gattcgggcc gaggctcgtg ccatcagggc
    10261 ggcggggccg ttctactgtg ggggagaatc ccagcttgct ttcgttcgcg tcgggccacc
    10321 acgcccattc gccgcgcgcg tcgcgcgcgt cccggaaaat ccggatcggc ctttatcttg
    10381 cctgcaccgc cctgagcgcg ccgctccagg cgggcgtgcc gacaatggcg gccgcggcgt
    10441 cgccgcagcc cgcgccggaa gcgcgcgagg agccgttcca ggatccggcc atagtcgcgg
    10501 cagcagccga cgaaggcgtg gtcgtggtgt cgcgggacga cgtctatatc ctcgaacagc
    10561 gagtggtgac ttcgggcgac gacagccccg ccatcttcgg ccgctcgacc ggcggcggcg
    10621 ttaccatcat caacggcgag gccgagacgg cgggcgacga ttccgtcggc atcttcgccg
    10681 aagcggaggg cgaaggccgc aacgccgacg tcaatgccgg gaccgtgatc acgcaaggcg
    10741 acaattcggg cggcataatg gtgcgcgccg agggcggcta cgtccaggat tcgccctacg
    10801 ggccgctcgc ctacggcaat gcaatcgtga ccagccagga cgtccgcacg tccggcgcga
    10861 attcgatcgg agtcgccgcg caatcgatcg gcggcgccgc tctggtgacc agcaaccagg
    10921 tcgccaccca gggcgacggc tcgatcggca tccaggcgat cggcgatgcg gtcgtgcagg
    10981 tcaccagcga aagcgtcacc accgccggcg tgggcgcgac cggtatcaac gcccagtcct
    11041 ccgagggcgg cgtcaccgtc cagagcggat cggtcgccac caccgacgcg ggcgcggccg
    11101 ggatcgtcgc ggaaggttat gccgacgtcg tcgtcatcag cgattccgtc tcgaccgccg
    11161 gcgatccggt cggcaaggag acgccggcgg cggtcggcat cctcgcctcc agcgaagccg
    11221 gcaacgtcgt cgtcgaaagc ggggccgtca gcacccaggg ccgcggcgcc accggcatcc
    11281 aggccaatgc ttatggcggc gtgacgatca ccagcgcctc ggtcaccgcg ctcggcgacg
    11341 gcgccaaggg gatcgacgtc agcaatgtca ccggcgccgt caacatcgac agcggcatcg
    11401 tcgacgtcag cggcgacctt gcccacggca tcaacgtcct cagcgagcag ggccgggtga
    11461 acatcaccag cgccgccgtg acgacgcgcg gcgacttctc ctcgggcatc aaggtggtgc
    11521 caggcggctc ggtcacgatc gacagcggca cggtcgacac gaccggcgac tatgcgatgg
    11581 gcatcgagat cttctcgacc ggcggcgacg tcaccgtcgc gagcggcgcg atcaccaccg
    11641 acggcacccg ctccaacggc atctcggcgg tggcgctgac cagcggcggc gacgtcgaca
    11701 tcgaggcgga tagcgtcgcg accggcggct tcagctccga cgccatttac gccagtgcgg
    11761 gatcgaacct caccgtcaag gcggggtcgc tgtcgacgcg cggcgaggaa tcgaccggcg
    11821 tcttcgccaa tgcctacgat tcggtcggca tcaccgttga aagcattgcc acccaaggcg
    11881 cgctgtcggc cggcgtctcc gcccagtccg agggcggcag cgtcgcggtc gaggtcggtt
    11941 cggtcacgac cgcgggcgat tcctcggtcg gcatctatgg cggcgcgctc gaggacgtca
    12001 ccatcgcggc gggcagcgtc gtcaccacgg gcgaccggct ggtgcgcacc gatcccgaca
    12061 ccggcgagac catctatttc gac
//
LOCUS       DASVRK010000133        31366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_397736, whole genome shotgun sequence.
ACCESSION   DASVRK010000133 DASVRK010000000
VERSION     DASVRK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 31366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31366
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397736"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF589_05045"
     CDS             complement(<1..392)
                     /locus_tag="VF589_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HEX8256977.1"
                     /translation="MPAYNKADLARRMHGAVEALKHDLGGLRTGRASISLLEPVHVEV
                     YGANMPLNQIATISVPEPRMLSVQVWDRSNVGPVEKAIRSAGLGLNPISEGQTLRLPI
                     PDLTEERRKELAKLASQYAEKAKIAVRNV"
     gene            complement(396..1118)
                     /gene="pyrH"
                     /locus_tag="VF589_05050"
     CDS             complement(396..1118)
                     /gene="pyrH"
                     /locus_tag="VF589_05050"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX8256978.1"
                     /translation="MPRPRFNRILLKLSGEALMGEGQFGIDPETTSRLATEIRQAREA
                     GHDLCLVVGGGNIFRGLAGAAKGFDRASADYMGMLATVMNALAMQNALEKIGVDTRVQ
                     SAIPMATVSEPYIRRRAVRHMEKGRVVIFAAGTGNPYFTTDTAAALRAAEMGCDALFK
                     GTSVDGVYDADPKKVAGASRYESLSFNRVLADDLKVMDASAVALCRDNNIPIVVFNVR
                     EQGNLARVLSGEGVATIVTNEE"
     gene            complement(1235..2194)
                     /gene="tsf"
                     /locus_tag="VF589_05055"
     CDS             complement(1235..2194)
                     /gene="tsf"
                     /locus_tag="VF589_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX8256979.1"
                     /translation="MAEITAANVKELRERTGAGMMDCKKALGETQGDMESAIDWLRTK
                     GLAAAAKKAGRTAAEGLVGVAVAGNRGAVVEVNSETDFVAKSEQFQEFVRTVAGLALT
                     ASGNVEGLLAANWPAGGSVQEKLTSNIATIGENQSLRRAAVLQVSEGVVVSYVHNAAA
                     PGLGKIGVLVALESSGDQAALEALGRQLAMHIAAANPLALTGEELDPTLIERERAIAS
                     EKAAESGKPADIVARMVDGAIAKYRKENALLSQLFVMDNKTKIEDVIAAEAKKIGAPV
                     SLNAYVRFQLGEGIEKKESDFAAEVAAAAGTARPADTREPVAS"
     gene            complement(2283..3041)
                     /gene="rpsB"
                     /locus_tag="VF589_05060"
     CDS             complement(2283..3041)
                     /gene="rpsB"
                     /locus_tag="VF589_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX8256980.1"
                     /translation="MASTDVSMQQLLEAGAHFGHQTHRWNPKMKPYIFGERNGVHIID
                     LSQTVPLFARALDFVRQAAASGGKVLFVGTKRQAQEPIADAARRSNQHFVNHRWLGGM
                     LTNWKTISNSIKRFKSLEEQLSGDTAGLTKKEVLQLTRERDKLEMSLGGIRDMGGLPD
                     VIFIIDANKEELAIKEANVLGIPVVAILDSNTSPQGIAFPVPGNDDASRAIRLYCDAI
                     ADAATAGRQGGQAARGVDIGAMDVPPAEAALADA"
     gene            complement(3424..4233)
                     /locus_tag="VF589_05065"
     CDS             complement(3424..4233)
                     /locus_tag="VF589_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEX8256981.1"
                     /translation="MRPPLPRRPKGIPLRSLVPNAVTALALCFGLTGVRFAIEEKWEL
                     AVLAIVIAAVLDGMDGRIARMLNGQSRFGAELDSLSDVIAFGVSPAVIIYLWSLQENP
                     RYFWLFALAHAVCAALRLARFNAAIDVEEQPHKSAGFLTGVPAPAGAGLLLLPLYLWL
                     WTDQEIFRAPLVVAPWAALIAFLMVSNVATFSWTSLRLRSHVRFGALAVIALIGGALI
                     SAPWPTLSLAAMLYAALIPASLYSYAKVKRQRVAAAARAQPPSPAADDPTS"
     gene            complement(4230..4964)
                     /locus_tag="VF589_05070"
     CDS             complement(4230..4964)
                     /locus_tag="VF589_05070"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX8256982.1"
                     /translation="MTSLEKPDLVTTNVRWRWPAVHPEGRKFVVIAAAIALAFLIFIS
                     DEIGWPLVLVTIWVAAFFRDPIRTTPKGDGLIVAPADGLVTMIAAVPPPREIAGEGGL
                     DEGLYSRVSIFMSVFDVHINRTPIAGEIKRVVYIPGKFLNADLDKASEDNERQHILVE
                     GADGLRIGFTQIAGLVARRIVPWVKPGDNVGAGQRVGLIRFGSRVDVYLPAGIAPQVI
                     LGQRTIAGETVIGQAGLSEPREGVSQ"
     gene            5134..6354
                     /locus_tag="VF589_05075"
     CDS             5134..6354
                     /locus_tag="VF589_05075"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX8256983.1"
                     /translation="MAKIKVTNPIVEIDGDEMTRIIWQWIRERLIQPYLDVELLYFDL
                     GIEKRDETEDRITIEAAHAIKKHGVGVKCATITPDEARVEEFGLKKMWKSPNGTIRNI
                     LGGVVFREPIVIENVPRLIPGWTDPIVVGRHAFGDQYKATDFRVPGPGKLRMVWEGED
                     GQRIEEEVFDFPSAGVAMGMYNLEDSIRDFARASLNYGLNRGWPVYLSTKNTILKAYD
                     GTFKDIFQEVFESEFKPRFDEAKIEYQHRLIDDMVASALKWSGKFVWACKNYDGDVQS
                     DLVAQGFGSLGLMTSILMTPDGKTVEAEAAHGTVTRHFRMHEQGKATSTNPIASIFAW
                     TGGLKYRGKFDDTPEVTRFAETLERVCVETVEQGQMTKDLAILIGPDQSWMTTEQFFE
                     AVRANLEKAMAQPA"
     gene            6459..7970
                     /locus_tag="VF589_05080"
     CDS             6459..7970
                     /locus_tag="VF589_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX8256984.1"
                     /translation="MSGYSRGMIRGQINNYPEGQFVAIYEGKISGYCATMRIDERLAL
                     APHSWDEITNDGFGSRHDPTGDWLYGFEMCVDPDVRGTRIGQRLYDVRKALTERLELK
                     GIVLGGRMPGYARSRRKVAGPEEYVEQVRAGKLRDPIIGFQLRNGFEPIGILKRYLPE
                     DRASAGNAVHMVWYNPYVDPDEPPKFRVPRDVESVRLATCQFQARAVASFEEFIRNVE
                     YFVDVASDYRSDFIVFPELFTLQLLAFEKKELSPAEAIDRLTEHTPNLVAELSRMALS
                     YNINIIGGSHPTRADDGDIQNVAYVCLRDGSVHSQEKIHPTPNERYWWNIKGGDRIDA
                     IQTDCGPIGVLICYDSEFPELARRLVDEGARIVFVPFCTDSRQGYLRVRYCSQARAIE
                     NQCFVVLSGNVGNLPNVENMDIQYAQSCILTPCDFPFARDGIAAEASENVETLTISDV
                     NLADLTWARAEGTVQNLADRRFDLYRIHWNANPRRDRPPPQVPTSGTDTPGGG"
     gene            8019..9800
                     /locus_tag="VF589_05085"
     CDS             8019..9800
                     /locus_tag="VF589_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8256985.1"
                     /translation="MATEFQTSGFSDALVILGAAGVVIPAFARFRISPVIGFILVGLL
                     VGPAGLGGMAANMPWLNYVTISNPEAVEPFAEFGVILLLFAIGLELSFRRLWTMRRLV
                     FGVGAAELLVSAVLIGLALHLIGQSWSGSIALGLALALSSTALVLPLVGTTSPVGRSA
                     LAMLLFEDLAIVPIIFALGAMAPYAQANGFDGLVETLLLGAAVVAAMLVLGRFLLPRL
                     FAQAARTKSPELFLAASLLVVIIASLATTAAGLSPLMGALLAGILIAETDYHSEVEVI
                     TAPYRGLALGIFLITVGMSVDLRLIAANWGSTLMAVVGVILVKALVTGLLLRLNGARK
                     AVAAEAGLLMASPSETTLIVLAAAASAQLIAREDAAFWQIVTAIGLTLTPLLSKLGQV
                     AARRVDLGAEPELPAIGTAKDAPTVVIIGFGRVGRLVAEMVKTHGRSYLAVDSDIDSV
                     AASRRDGFPVVFGDVSRPELVDRLNLGHAAALVLTMDDPVLTVRLTRKVRAWCPDLTI
                     VARARDAAHAAELYRAGATDAVPETLESSLQLSEAVLVDLGIAMGPVIASIHEKRAEL
                     RKEIMDLGEMDREPQLQRRRLRDALRR"
     gene            complement(9801..10283)
                     /locus_tag="VF589_05090"
     CDS             complement(9801..10283)
                     /locus_tag="VF589_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256986.1"
                     /translation="MLVQLMALLALHTSQTRIVAVGGVPNAVPAEARVAPEIIAVRVE
                     AAGAVLWDGTLRIAPNQAASYQQHLNQASAFQCPPDTPHDRSERRQLTVNIYANETPQ
                     QGRSYRVDASWQRPIPQQACLEGGTRTVQITQAVSLTPGETKTIEGDAGLRVQLTLRR
                     "
     gene            complement(10359..13016)
                     /gene="alaS"
                     /locus_tag="VF589_05095"
     CDS             complement(10359..13016)
                     /gene="alaS"
                     /locus_tag="VF589_05095"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX8256987.1"
                     /translation="MTSTNDIRRSFLDHFGAAGHRMVPSAPLVPHNDPTLMFVNAGMV
                     PFKNVFTGLETRPYSTAASSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KEQAIVHAWTLLTKQWGLSPDRLTATVYHTDDEAFELWRKVAGLPEQRIIRIPTKDNF
                     WAMGDSGPCGPCSEIFYDHGNHIPGGPPGSPDEDGDRFVEIWNLVFMQYEQEDNEIVR
                     ELPRPSIDTGMGLERIAAVLQGVHDNYDTDTFKALIAASGELTRTATTGERQASHRVI
                     ADHLRASGFLVADGVLPANEGRGYVLRRIMRRAMRHAHILGAAEPLLHRLVPELVAEM
                     GAAYPELLRAQPLIEATLRQEETRFRQTLDKGLRLLDEATASMAPGETLAGDVAFRLY
                     DTFGFPYDLTEDALRAQGFGVDRAGFDAAMAEQKAAARAAWKGSGAKASDDLWFDIAE
                     EAGSTEFTGYTAEEGEGAVVAIVRDGARVEQAGESEAVMIVTNQTPFYAESGGQVGDT
                     GEIVSDGGLRARVTDTSKQLGRLHVHHAVVEAGRVAVGDPVKLRIDGGRRSAIRANHS
                     ATHLLHAALRERLGKHVAQKGSLVAPDRLRFDVSHPSAMSAADLATVEDDVNFQVRAN
                     NPVTTRLMTPEEAIAEGAMALFGEKYGDEVRVVSMGTDEGRYSVELCGGTHVSALGDI
                     GLFKVIGEGAASSGIRRVEALTGDAARAYLNGRDEKLRAAAAVLKSSPDEVPARVAAL
                     VEERRRLERELADAKKALALGGGRASEAAGPELVGGHGFIGQVIEGLDPKGLRGLVDE
                     AKQRVGSGVVALVAVNEGRATVAVGVTDDLTPGLSAVDLVRAAVAELGGQGGGGRPDM
                     AQGGGPDGGKAEAALAAVRSALGEQVEAV"
     gene            complement(13083..13592)
                     /locus_tag="VF589_05100"
     CDS             complement(13083..13592)
                     /locus_tag="VF589_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8256988.1"
                     /translation="MTLPTVAMLALAGTGLGVYLGRAAVDEINPLYFSLPEATRSYAA
                     LTPNRGDDFDALRPREVAATEYGAALGRGCVGCRTYPEEYFPRRDPAVDAQLERSWSD
                     EEPAVQQAVQQLPQFAAARSEEQRLIEAYSRYPVDEEEMRRHERALEVVVEPVAEAQA
                     EAEPVPVGL"
     gene            13817..15853
                     /locus_tag="VF589_05105"
     CDS             13817..15853
                     /locus_tag="VF589_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8256989.1"
                     /translation="MPERSVGTRMVALVGPGGAGKTSLAEALLFAAGTIDRQGSVDGR
                     SSVGDASPEARARCGSTEINLMRLEFLGDRFAIVDCPGSVGFAADGAMAVAAADIAIV
                     VVDPDPARALLAEPALRQLEALGIPHAIFVNKIDQARGSIHDLLEALQPMSNSPLIAR
                     QIPIRGKDAGGEKITGFVDLALERAFHYRAGKPSERTEIPADLAEREALERYHMLEQL
                     ADHDDALLEQLLMDETPDLPTVLADLARETRENLGVPVLFGSALNGFGIRRLLKALRH
                     EAPAPADAAARLGASDGCAFVFKISHGGAIGRLALARVFGGALSEGTELKGPDGSAVR
                     VGTLFALQGEKTAKLVKAEMGEVVAIAKAESVHAGGWLSLGKSAPAPAVELPASNYAL
                     AIVTRDRKDDVRLSTALHKLVEEDPSLSWEQDEALHETRLKGVSDEHLNVTIARLKRR
                     YGVAVDSKPPTIGYKESIRRQVTQRGRHKKQSGGHGQFGDAVIEVKPLPRGGGFAFDE
                     RIHGGAVPRQYIPAVEQGVRDAMLKGPLGFPVVDVAVTLTDGSYHSVDSSELAFRTAG
                     RIAMSEALAAAQPHLLEPVHRVTVVAPGSATSKVTSAISSRRGQMLGMEPREGWSRWD
                     KVEALMPESELQGLDAELRSLSQGLASYTAEFDHLAELNGKLAEEVVQKVLEPA"
     gene            complement(15855..17180)
                     /locus_tag="VF589_05110"
     CDS             complement(15855..17180)
                     /locus_tag="VF589_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8256990.1"
                     /translation="METTHRGVPLQMKMLIGFVVGLSAGLLVHVTQPDAPWVEAVTTY
                     VTGPIGQIFLRLLFMLVIPLLFSALVVGIAEMGDIRSLKRVGIKTLIFTIGISAVAVV
                     IALAVVNIFNPGGGVDPVLAQSLLEDAREGAGAIVSKSAETPSGVAAFLNIIPNNIVA
                     AAADNDILAVMFFALVFGIGLLLVNSPSAQRLQEAIEGIFHVAMRLIGIVIRLAPIAI
                     ACFMFNLAAVFGWDLLLRLGAYVGVVLLALGIHMIVVYSLAIRFLGGKSPLWFFRQSQ
                     EAMVMAFSTASSNATLPTALRVADEKLHLPPRVSRFVLTIGATANQNGTAMFEGVTVI
                     FLAQFFGVELTLAQQVMVLLVCILGGIGTAGVPAGSLPVVALILGMVGVPPEGIGLVL
                     GVDRFLDMCRTTLNVTGDLAAAVVISRGEEDAPAAAPIADAATVEAHPS"
     gene            complement(17298..18566)
                     /locus_tag="VF589_05115"
     CDS             complement(17298..18566)
                     /locus_tag="VF589_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX8256991.1"
                     /translation="MPMPELGRRAFTLSALAAGGALIGGSRIMAAAPVPSALAPITGD
                     AKPIGRDERLQRLAGAQRLMRAQGLGALLIEPGASLDYFTGVQWWRSERLTAALIPAE
                     GEPIVFTPFFEEPSVRESLAVPAAVRTWTEHEDPLALVAAALRERKLATRPVGMEETV
                     RFFAVDGLRKALPSVEIRSGAPVVRALRMVKSPAELALMQKATDVTLAAYAHTAPRIA
                     AGMTPAQISAIMNEATVALGGSPEFALALLGEASAYPHGSGKPQQVREGEVVLMDCGC
                     TVHGYQSDVSRTFVFGGKATGEQRKVWDQVHRGQQIAFAAARPGAPAGSVDDAVRAQY
                     QEWGYGPGYKLPGLSHRTGHGIGMEGHEPVNLVHGETTPLAPGMCFSNEPGLYLPGRF
                     GVRIEDCFYMTPEGPHWFSTPPPSIDRPFG"
     gene            complement(18553..19200)
                     /locus_tag="VF589_05120"
     CDS             complement(18553..19200)
                     /locus_tag="VF589_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8256992.1"
                     /translation="MIIVHHLENSRSQRILWLLEELGLPYEVRRYERDPKTMLAPPEL
                     RRVHPLGKSPVIEDAGSGSEAGERRVLAETGAIVEYLVEKGDGKLGPPARREGVLRYR
                     HFLHYAEGSMMPPLLALLVVGRLGILGKPARAPIQKMLDDHLDWLEAELAERDWFAGD
                     EFSAADIMMSFPLEASRSRGGLDESRPHLIDWLERIHARPAYAAALQKGGPYAYA"
     gene            complement(19197..20015)
                     /locus_tag="VF589_05125"
     CDS             complement(19197..20015)
                     /locus_tag="VF589_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX8256993.1"
                     /translation="MTLDTRPRLRLIGLPTDSHSSFLRGAAAAPPLIRAALASDHGNQ
                     NAESGLELGTDIWLEDGGDLPLAEAEGDHALIRDATAAACSQGAVPLCLGGDHYVSFP
                     AVEGVAAVHGPVDILHFDAHPDLYDDFEGDPHSHASPFARILERGLARRLVQVGIRTL
                     NRHCREQAERFGVEVVEMRHFAPDRVPIPSGPLYISIDMDGFDPAFAPGVSHHEPGGL
                     SVRDVLAVLDRVEAPIVGADIVEYNFSRDLNGVTAVLAAKLVKEVAALCARTSR"
     gene            20128..21405
                     /locus_tag="VF589_05130"
     CDS             20128..21405
                     /locus_tag="VF589_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX8256994.1"
                     /translation="MSDREAAPPAADWRGPGLREFVALVAALMASNALAIDAMLPALP
                     AIGEALGVAEDNRRQLVITAYLLGFGVAQLAYGPLSDRYGRKRLLVGALLFYGVFAAL
                     AGIAGSFTLLLGARALQGVAAAATRVLVVSVVRDRYQGSAMAQIISTVMIVFMIVPVL
                     APSLGQGVLAVASWRHIFIFLAAYGVILALWTGLRLPETLRPEHRRALSVARIAEAAW
                     MTLGNRQSIGNTLAQTLVMGALFAFINSIQQIVFDVFRRPELIGLVFACIAGPMALSS
                     YANSKLVMRWGSKRLLLLALAAFTAVSALHLAVAAAAGEAIWTFVILQAATMAFFGLI
                     GANAGALAMEPLGHIAGTASSLQGVITTVGGALIGLAIGQSFNGTTLPFLAGFAMCGM
                     AALATAFWANRPPSLDSRDRAEVEVQETAARPG"
     gene            complement(21491..22150)
                     /locus_tag="VF589_05135"
     CDS             complement(21491..22150)
                     /locus_tag="VF589_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8256995.1"
                     /translation="MSKIVNGSSLQPLSGAKPRQIVLLLHGYGSNGADLISLAPHWRQ
                     ALPEALFLAPNAPQTMGMGGAYQWWGLNAFTPQALAAGAQSAAPALDAFIDRKLDQYG
                     LTEADLALVGFSQGTMMALHVGLRRARPAACILGYSGMLTGAAELSHHLRSKPPVLLI
                     HGSADPIVPVAALHAAKSDLQRLGVEVTTHVSSGLGHSVDPVGLRLGGQFVVKALGQA
                     G"
     gene            complement(22218..23237)
                     /locus_tag="VF589_05140"
     CDS             complement(22218..23237)
                     /locus_tag="VF589_05140"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8256996.1"
                     /translation="MHAPLSTNTAIDPRALMGEIGPFRQSRTARSLFELAITAAPFTL
                     LWLLTWAALDAGYMAGLLLTVPAGAFLLRLFLIQHDCGHGAFFRRQASNDWLGRILGV
                     LTLTPYDYWRRSHAIHHASTGNLDARGVGDVDTLTVAEFNALSRAGRFWYRVYRHPLV
                     LFGLGPAYLFLLRHRLPIGMMKRGWRPWLSALGTNASFAAVAGALIWAMGWKLFLLVH
                     LPITMVAASLGVWFFYVQHQFEQTHWDRGEDWSFHAAALHGSSHYDLPPVLRWFSANI
                     GVHHVHHLASRIPFYRLPEVLREVPMLARISRVTFRESIGAVRLVLWDEQKRRLVTFA
                     EARCG"
     gene            23577..24005
                     /locus_tag="VF589_05145"
     CDS             23577..24005
                     /locus_tag="VF589_05145"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX8256997.1"
                     /translation="MKTTLLAALIAALAAPAGAHAAPAGSSRLTLTFETGAATGAVLV
                     ALFDSKAAYDGGAPVRQARVDVAKGERIAIFANLASGDYAMKAFHDLDGDGRMTTNPF
                     GRPIEPFAFSNNARGNMGPAAWDRARLKVAADVSQIVEIR"
     gene            24005..25483
                     /locus_tag="VF589_05150"
     CDS             24005..25483
                     /locus_tag="VF589_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX8256998.1"
                     /translation="MQLQDTQLVFSRRAMMLGAASLVATPDMSWAAKALAIASDWSLA
                     TADVEADLQPRALRLASGRAPKGLEGSLYRNGPAKFRRPGRSAGHWFDGDGLIRRFRI
                     ADGEASLAARFADTPKRRLETRLGAMVMPGFGSASDPRAELRGPDDANAANTSVLPVG
                     DKVWALWEGGSPMALDGATLASEGFVTLRPDLKAMPFSAHPRIEPDGRVWNLGINGKQ
                     AMVWRLSAAGALEAAELIELPRASYLHDFTATARHLVVVLQPWIHHREVFPLARGYSW
                     RPETGTQVLIIDKADLSRRRIVELPPFGFFHLGDAWEEADGTIRFDVCAYPDMRFAAS
                     GASDLLDSQYDGGGWSEMVLAMLPLRGPGRLERTSVGAEFPKGDARRAGLPRRFSVHT
                     AGDTPGRPMATGVGVTDWRSGRTRTFDFGPNHVVDEMVPVPKPGSTAEDQAWLVGPTI
                     NLKAGVTELHVLDMARVEDGPIATWAADVALPASFHGRWQSA"
     gene            complement(25488..26234)
                     /locus_tag="VF589_05155"
     CDS             complement(25488..26234)
                     /locus_tag="VF589_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8256999.1"
                     /translation="MTPERRDLVRNLGVAAAAALVLAVSGAFGTGAELFHERLTYWFM
                     AMAVGSAWGMLCQRLLARRMELEERPWLHIGVLTAIVAGPMCLFVWAATGLFFHGELY
                     PVRSLPLVLVPVVTVTAAVCALVVLLGRAAPVQTHAAADAGKPVKFIERLPLRLRGSR
                     LIAVEAEDHYLRVHTADGSHLILMRLSDALDELEGLEGAQTHRSWWVARDAVRDAQRG
                     DGRATLTLDGGLTAPVSRRYARALREAGWY"
     gene            26392..26664
                     /locus_tag="VF589_05160"
     CDS             26392..26664
                     /locus_tag="VF589_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257000.1"
                     /translation="MNFEDQLQRYFGTADIASISPAALGAAVERMRVDLGLETDRGRR
                     FALWALLHMLGSAPDLDVAFPDPADRDAARNFMELSEQAPPGAAGD"
     gene            26774..30649
                     /locus_tag="VF589_05165"
     CDS             26774..30649
                     /locus_tag="VF589_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257001.1"
                     /translation="MATYNGGKYNDWYVGTDSADTAHGNAGDDRIETRGGNDRLYGGS
                     GSDQLYAGIGDDLLDGGTGADYLDGDDGNDTYVVDHVDDRIVERSGIDTVQAAFHYVL
                     AAELENLTLTGTAALGGFGNDFANVIAGNGAANRLDGGLGDDTLTGGAGADSFVFSTA
                     LNVGPNLDRITDFSVADDTILLDRSVFTGLGANGAIAASAFVNGATAADGDDRILYDR
                     ATGNIFYDSDGSGAGVKFLFARVTAGTALTSADFVATGGAAGTLYTGDAADNLFSGSG
                     GDDQASGHGGNDLLLGGGGSDRFDGGEGTDRIYGGDGGDHLDGGTGSDYLHGGRGDDT
                     YVVDFESVDSRYPTDRVVEAANEGVDTVLSSLRSYALTSFVENLTLTGTAYQGIGNAL
                     DNVITGTSANNRLSGERGADRTIGGAGDDFYWVDNAGDAVVELAGEGVDAVQSSVTHR
                     LADHVENLSLVFQDSPYGAVRDFDGYGNALANRISGSVYNNLIDGGLGNDTLTGGAGA
                     DRFLFSTALNAETNADLITDFSVGYDMIVLDRAVFTAIGAEGPLSASAFVNGVTALDA
                     DDRILYAKETGYIFYDADGSGAGAKILFAKVTAGTALSNVHFSAVGGEPPVYGGTGPT
                     YVGDAGDNMFVGGNEADEAEGNGGNDRLQGNGGDDILDGGAANDTLEGGIGADTLRGG
                     DGSDRLSSSVTPNTYGDGWQPASPDTGAEADTVFGGAGDDQIFAGYGDSVDGGADTDR
                     LYISFQGATAGVQADFSKLYSGGSMTIGGATISGIEAVIHVEGSNFDDFIAGTSGEFN
                     GTVYGRGGNDHILAAFSSGLIYGGDGDDLIDSSATVTSYSHYGEAGNDRLIGGELTNK
                     LYGGEGNDFLDGGRQADYLFGGAGDDTYVVDVGGSHPEDAVRDQVSEAAGEGTDTVLA
                     SATYWLAANVENLTLTGASAIGAHGNGLDNVLTGNAAANRMYGAGGADRMSGGAGDDD
                     YQVENAGDLVVEAAGEGIDAVQSEISYTLTDKVETLLLVGTRTSMGTYVDQAIDGTGN
                     ALDNQLYGNSASNILDGGAGADLVVGGAGNDRLAGGSGDDRLEGGDGNDIYTVASADD
                     LLVELAGQGSDTARASISYTLGDTVEHLALLGRAALDGTGNRLNNRLTGNVGDNRLDG
                     GAANDVLAGGLGNDILIGGDGGDRLTGGTGQDLLTGGLGADIFLFVEEADSPVAAGDA
                     IADFAQGLDKIDLTGIDADALAAGDQAFTFIGTEAFTGGGAAGAGQLRYEQVDGRTLI
                     SGDVDGDGAADFLIRLDSPAPLQATDFLL"
     gene            30881..>31366
                     /locus_tag="VF589_05170"
     CDS             30881..>31366
                     /locus_tag="VF589_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257002.1"
                     /translation="MSVSEFSRRRAFAASVSLAALAMASAAAPALAQEACGPVAEGVV
                     TCDEADAPFPQGIAYSTSEDLTVVVEDGLTVAPDAGVAGVILSSEGSVTLQAGGADVV
                     ANGASAIFAAGSDDMTIVVDDAAATGDYGRGIDTFTFWGDTEIVADSVSTSGRDALGI
                     TA"
BASE COUNT         5018 a        10572 c        10965 g         4811 t
ORIGIN      
        1 acgttgcgga cggcgatctt tgccttttca gcatattggc tggcgagctt cgcaagctct
       61 ttgcgccgct cctcggtcag gtcagggatc ggcagccgca gcgtctggcc ttcgctgatc
      121 gggttgaggc cgagccccgc cgaacggatc gccttttcga ccggaccgac gttggagcgg
      181 tcccacacct ggacgctcag catgcgcggc tcgggcacgg agatggtcgc gatctggttc
      241 aatggcatgt tggcgccata gacttcgaca tggaccggct cgagcaggct gatcgaggcg
      301 cggccggtgc gaaggccgcc gagatcgtgc ttcagcgcct cgaccgcgcc gtgcatgcgg
      361 cgcgcgagat cggctttgtt ataggcgggc atcgctcact cctcgttcgt gacgatcgtc
      421 gccacgcctt cgccgctgag cacgcgggcg aggttgccct gctcgcggac gttgaacacg
      481 acgatcggga tattgttgtc gcggcacaaa gccaccgcgc tggcgtccat caccttgagg
      541 tcgtccgcca gcacccggtt gaagctcagg ctctcatagc gggaggcgcc cgccaccttc
      601 ttcggatcgg catcgtagac gccgtcgacg ctggtgccct tgaacaaagc gtcgcagccc
      661 atttcggcgg cgcggagggc ggcggcggtg tcggtggtga aatagggatt gccggtgccg
      721 gcggcgaaga tcaccaccct gcccttctcc atgtgccgca cggcgcggcg gcggatatag
      781 ggttcggaca cggtcgccat cgggatcgcc gactggacgc gggtgtcgac gccgatcttc
      841 tcaagcgcat tctgcatggc gagtgcgttc atcacggtcg cgagcatgcc catataatcg
      901 gcgctggcgc ggtcgaagcc cttggcggcg ccggcgaggc cgcggaagat attgcctccg
      961 ccgacgacca ggcagaggtc gtggccggcc tccctggcct gcctgatctc ggtcgcgagg
     1021 cgcgaagtgg tttcgggatc gatcccgaac tggccctcgc ccatcagcgc ctcgccggaa
     1081 agcttcagga ggatgcggtt gaagcgcggg cgcggcattg aaattcctgt ccgatcggcg
     1141 gctcgcgggg ggcggatggc gcgcacctta gtaagcgcgc gccaccctgc caagtgttcc
     1201 ggctattccc ctcgcacagg cgggagcgag gcgcttagga agcgaccggc tcgcgcgtgt
     1261 cggccggcct ggccgtgccg gcggcggccg caacctcggc ggcgaagtcg ctttccttct
     1321 tctcgatgcc ctcgccgagc tggaagcgga cataggcgtt gagcgacacc ggagcgccga
     1381 tcttcttggc ctcggcggcg atgacgtcct cgatcttggt cttgttgtcc atcacgaaga
     1441 gctggctcag cagcgcattc tccttgcggt acttggcgat ggcgccatcg accatgcggg
     1501 cgacgatgtc cgccggcttg cccgattcgg ctgccttttc cgacgcgatc gcgcgctcgc
     1561 gctcgatcag ggtcggatcg agctcctcgc cggtgagggc gagcgggttg gcggcggcga
     1621 tgtgcatcgc cagctgtcgg ccgagcgctt ccagcgcggc ctggtcgccg gacgattcga
     1681 gcgcgaccag cacgccgatc ttgccgagtc cgggcgccgc cgcattatgg acataggaga
     1741 cgaccacgcc ctcgctcacc tgcagcacgg cggcgcggcg gagcgactgg ttctcgccga
     1801 tggtggcgat gttcgaagtg agcttctcct gcacgctgcc gccggccggc cagttggcgg
     1861 cgagcaggcc ctcgacattg ccactcgcgg tcaaagcgag gccggcgacg gtgcggacga
     1921 actcctgaaa ctgctcgctc ttggcgacga agtcggtctc cgaattgacc tcgacgacgg
     1981 cgccgcgatt gccggcgacg gcaacgccga ccagtccttc ggcggcggtg cggccggcct
     2041 tcttggcggc ggcggcgagg cccttggtgc gcagccagtc gatcgccgat tccatgtcgc
     2101 cctgggtctc gcccagcgcc ttcttgcaat ccatcatgcc ggcgccggtg cgctcgcgca
     2161 gttccttgac gttcgcggca gtgatctccg ccatttcgat ttccttttga ttgcctgttc
     2221 cccggcgcaa gccggggccc aaaggtcttc taagctggac cccggcctgc gccgggaaac
     2281 gatcaggcgt cggcgagcgc cgcttcggcg ggcggcacgt ccatggcgcc gatgtcaacg
     2341 ccgcgggcgg cctggccgcc ttggcggccg gccgtggccg catcggcgat cgcgtcgcaa
     2401 tagaggcgga tcgcgcggct ggcgtcgtca ttgcccggaa ccgggaaggc gatgccctgc
     2461 gggctggtgt tcgaatcgag gatcgcgacg acggggatgc cgagcacatt ggcttccttg
     2521 atcgccagct cttccttgtt ggcgtcgatg atgaagatga cgtcgggaag gccgcccatg
     2581 tcgcggatcc cgccgagcga catttcgagc ttgtcgcgct cgcgggtcag ctggaggact
     2641 tccttcttgg tcaggcccgc cgtgtcgccc gacagctgct cttcgaggct cttgaagcgc
     2701 ttgatcgagt tggagatcgt cttccaattg gtgagcatgc cgcccagcca gcgatggttg
     2761 acgaaatgct ggttcgagcg gcgcgcggcg tcggcgatcg gctcctgcgc ctggcgcttg
     2821 gtgccaacga acagcacctt gccccccgaa gcggccgcct ggcgcacgaa atcgagcgcg
     2881 cgcgcgaaca gcggcacggt ctgcgacaga tcgatgatgt gaaccccgtt ccgctcgccg
     2941 aaaatatacg gcttcatctt ggggttccag cggtgggtct ggtggccgaa atgcgctccg
     3001 gcctcgagca attgctgcat ggagacgtca gtggacgcca tggtctttct ccttccggtt
     3061 atgcctctgc ggggccgaga aactccggac caggtccaga gcaccggtat gatggcaccg
     3121 catgtgggat gccggcgcac ttagcggtgc ggccgcccga aatcaagcct tgacctgaga
     3181 acgaaacatg aacatcaaca acattcaacg ggctaggaaa ctaatccaaa gcagaatcgt
     3241 tgagttgtgg ataaataagg ggataagctg tgggccacct cgttagcata gtcttttttg
     3301 ccctcgtcct gacgggcgcc gccttcacca tcagcctcac gcttcgcgaa tattgggccg
     3361 agatcatcgg cgcgctcaag ggcgagatgc ccgtgcgcca tgctcagcgt ccgtggagcc
     3421 gggtcaggat gtcggatcgt ccgcggccgg tgacggtggc tgtgcgcgcg cggcagccgc
     3481 aacgcgctgc ctcttgacct tcgcatagct gtagaggctg gccgggatca gagcggcgta
     3541 aagcatcgcc gccaggctga gcgtcggcca cggcgccgag atgagcgcgc cgccgatcaa
     3601 ggcgatcacc gccagcgccc cgaaccggac atggctgcgc agccgcagcg aggtccagga
     3661 gaaggtggcg acattcgaga ccatcaaaaa ggcgatcagg gccgcccaag gcgcgacgac
     3721 cagcggcgcg cggaaaatct cctgatcggt ccacagccat aaatagagcg gaagcagcag
     3781 tagcccggcg ccggccgggg cgggtacgcc ggtgaggaag ccggccgact tatgcggctg
     3841 ctcctcgacg tcgatggcgg cgttgaagcg cgccagccgg agcgccgcgc aaaccgcatg
     3901 ggcgagcgcg aacagccaga aatagcgcgg attttcctgc agcgaccaca gatagatgat
     3961 caccgctggc gatactccga aggcgatcac gtcggacagc gaatcgagct ccgcgccgaa
     4021 ccggctctgg ccgttcagca tccgcgcgat ccgcccgtcc atgccgtcga gcacggccgc
     4081 gatcacgatc gccagcaccg ccagctccca tttctcctcg atcgcgaacc ggacgccggt
     4141 gaggccgaag caaagcgcca gcgcggtgac cgcgttcggc accagcgagc gcagcggtat
     4201 ccccttgggc cgccgcggca gcggaggcct cactggctga cgccctcgcg cggctcggaa
     4261 aggccggcct ggccgatcac cgtctcgccg gcgatggtgc gctggccgag gatcacctgc
     4321 ggcgcgatgc cggccggaag atagacgtcg acgcggctgc cgaagcggat caggccgacg
     4381 cgctgcccgg cgccgacatt gtcgccgggc ttgacccacg gcacgatccg gcgggcgacg
     4441 aggccggcaa tctgggtgaa gccgatgcgc aggccgtcgg ccccttcgac caggatgtgc
     4501 tggcgctcat tgtcctcgct cgccttgtcg agatcggcgt tgaggaactt gccgggtata
     4561 tagaccaccc gcttgatctc gccggcgatc ggggtccgat tgatgtggac gtcgaacacg
     4621 ctcatgaaga tcgagacgcg gctgtagagg ccctcgtcga gacccccctc cccggcaatt
     4681 tcgcgcggcg gcggcaccgc cgcgatcatc gtcaccagcc cgtcggcggg agcgacgatc
     4741 agcccgtccc ctttgggcgt ggtgcggatc ggatcgcgga agaaggcggc gacccagatc
     4801 gtcacgagga cgagcggcca gccgatctca tccgagatga agatcagaaa ggcgagtgcg
     4861 atcgcggccg cgatgacgac gaacttgcgc ccttcgggat ggacggcggg ccagcgccaa
     4921 cggacgttgg tggtgacgag gtccggcttt tcgagcgatg tcatcggagg acccttaagc
     4981 cgcgcggaag gcggagacaa tggtgagagg cgggggcgag cgcgggggcg agagcgccac
     5041 cgccgcggtt gatccatgcg ccgcctcgcc ctacacgccc ggacactcag caacccgcca
     5101 cgcgccccag ccagcaagga aagacgcccg ccgatggcca agatcaaagt gaccaacccg
     5161 atcgtcgaga tcgacggcga cgagatgacc cggatcatct ggcaatggat ccgcgagcgg
     5221 ctgatccagc cctatctcga cgtcgaactg ctctatttcg atctcggcat cgagaagcgc
     5281 gacgagaccg aggaccggat cacgatcgag gcggcgcacg cgatcaagaa gcacggcgtc
     5341 ggcgtcaaat gcgcgacgat cacgcccgac gaggcgcggg tcgaggaatt cggcctcaag
     5401 aagatgtgga agtcgcccaa cggcaccatc cgcaacatcc tcggcggcgt cgtctttcgc
     5461 gagccgatcg tcatcgaaaa cgtgccccgg ctgatcccag gctggaccga cccgatcgtg
     5521 gtcggccgcc atgccttcgg cgaccagtat aaggcgacgg acttccgcgt ccccggcccc
     5581 ggcaagctgc gcatggtgtg ggaaggcgag gacggccagc gaatcgagga ggaggtgttc
     5641 gacttcccgt ccgccggcgt cgcgatgggc atgtacaatc tcgaggattc gatccgcgac
     5701 ttcgcccgcg ccagcctcaa ttacggcctc aaccgcggct ggccggtcta tctttcgacc
     5761 aagaacacca tcctcaaggc ctatgacggc accttcaagg acatcttcca ggaagttttc
     5821 gagagcgagt tcaagccgcg cttcgacgag gccaagatcg aatatcagca ccggctgatc
     5881 gacgacatgg tcgcctccgc gctgaagtgg agtggcaagt tcgtctgggc ctgcaagaat
     5941 tacgacggcg acgtccagtc cgacctcgtc gcccaggggt tcggctcgct gggcctgatg
     6001 acctcgatcc tgatgacgcc cgacggcaag acggtcgagg ccgaggcggc gcacggcacc
     6061 gtcacccgcc acttccgcat gcacgagcag ggaaaggcaa cctcgaccaa cccgatcgct
     6121 tcgatcttcg cctggaccgg cggcctgaaa tatcgcggca agttcgacga cacgcccgaa
     6181 gtcacccgct tcgccgaaac gctcgagcgg gtctgcgtcg agaccgtcga gcagggccag
     6241 atgaccaagg acctcgccat cctgatcggc cccgaccaga gctggatgac gaccgagcaa
     6301 ttcttcgagg cggtccgcgc caaccttgaa aaggcgatgg cgcagcccgc gtgaagcccg
     6361 accgatcaga ccgccggacc gccaagctcg aggtcaggca ggcgcggccg gccgacgtcg
     6421 gcggcatcgt cgcgctggtc gaccgcgctt acgaccgcat gtcgggatac agccgcggca
     6481 tgatccgcgg ccagatcaac aattatcccg agggccagtt cgtcgcgatc tacgagggca
     6541 agatctccgg ctattgcgcg acgatgcgga tcgacgagcg gctcgcgctc gccccgcaca
     6601 gctgggacga gatcacaaac gacggtttcg gatcgcgcca cgatccgacc ggcgactggc
     6661 tctacggctt tgagatgtgc gtcgatcccg acgtgcgcgg cacccgcatc ggccagcggc
     6721 tctacgacgt ccgcaaggcg ctgaccgagc ggctcgagct caagggcatc gtgctgggcg
     6781 ggcgaatgcc gggctatgcg cgcagccgcc gcaaggtcgc cgggccggag gaatatgtag
     6841 agcaggttcg cgccggcaag ctgcgcgacc cgatcatcgg cttccagctg aggaacggct
     6901 tcgagccgat cggcatcctc aaacgctacc tgccggaaga ccgcgcctcg gccggcaacg
     6961 cggtccacat ggtttggtac aatccctatg tcgatcccga cgagccgccc aagttccggg
     7021 tgccgcgcga cgtcgagagc gtgcgcctcg cgacctgcca gttccaggcc cgcgccgtcg
     7081 ccagcttcga ggagttcatc cgcaacgtcg aatatttcgt cgacgtcgcc tcggactacc
     7141 gctccgactt catcgtgttt ccggagctgt tcacgctcca actgctcgcc ttcgagaaga
     7201 aggaattgag ccccgccgag gcgatcgacc ggctgaccga gcatacgccc aatttggtcg
     7261 ccgagctgtc gcggatggcg ctcagctata acatcaacat catcggcgga tcgcacccga
     7321 cccgcgccga cgatggcgac atccagaacg tcgcctatgt gtgcctgcgc gacggttcgg
     7381 tccattcgca ggagaagatc cacccgaccc ccaacgagcg ttattggtgg aacatcaagg
     7441 gcggcgaccg gatcgacgcg atccagaccg attgcggccc gatcggcgtg ctcatctgct
     7501 acgattcgga atttcccgag ctcgcccgcc ggctggtcga cgagggcgcg cgcatcgtct
     7561 tcgtcccctt ctgcaccgac agccgccagg gttacctccg cgtccgctat tgcagccagg
     7621 cgcgcgcgat cgagaaccaa tgctttgtcg tcctgtccgg caatgtcggc aacctcccaa
     7681 acgtcgagaa tatggacatt caatatgcgc aaagctgcat cctgacgccc tgcgacttcc
     7741 cgttcgcgcg cgacggcatc gccgccgagg cgagcgagaa tgtcgagact ctgaccatct
     7801 ccgacgtcaa tctcgccgac ctcacctggg cgcgggccga gggcaccgtc cagaacctgg
     7861 ccgaccggcg tttcgatctg taccggatcc actggaacgc caatccgcgc cgcgaccggc
     7921 ctcccccgca ggtgccgacc agcggcaccg acacgccggg cggcggataa agcggagctc
     7981 atggctgaca ggccggcggt ttgcggcata agggcgatat ggcgactgaa ttccagacct
     8041 cgggcttcag cgacgcgctg gtgatcctcg gcgcggccgg ggtcgtgatc ccggccttcg
     8101 cccgcttccg gatcagcccc gtgatcggct tcatcctggt cggcctgctg gtggggcccg
     8161 ccgggctcgg cggcatggcg gcgaacatgc cgtggctgaa ctacgtcacc atctccaatc
     8221 ccgaagcggt cgagccgttc gccgagttcg gcgttatcct gctgctgttc gcgatcgggc
     8281 tcgaactctc cttccgacgt ttgtggacga tgcgccggct ggtgttcggc gtcggcgcgg
     8341 ccgagttgct ggtctcggcg gtgctgatcg gcctggcgct tcacctgatc ggccagagct
     8401 ggagcggctc gatcgcgctc gggctcgcgc tcgctttgtc ctcgacggcg ctggtgctgc
     8461 cgttggtcgg caccaccagc ccggtgggcc ggtcggcgct ggcgatgctg ctgtttgagg
     8521 atctggcgat cgtgccgatc atcttcgcgc tcggcgcgat ggcgccttat gcgcaggcga
     8581 acggcttcga cggcctggtc gagacattgc tgctgggggc cgcggtggtg gcggcgatgc
     8641 tcgtgctcgg ccgcttcctg ctgccgcggc tgttcgcgca ggcggcgcgc accaagagcc
     8701 ccgaactatt cctggcggcg agcctgctgg tggtgatcat cgccagcctc gccaccaccg
     8761 ccgccggcct gtcgccgctg atgggcgcgt tgctcgccgg catcctcatc gccgagaccg
     8821 actatcatag cgaggtcgag gtgatcacgg cgccgtaccg cgggctcgcg ctcggcatct
     8881 tcctgatcac ggtggggatg agcgtcgacc tgcgcctgat cgccgccaat tggggcagca
     8941 cgctgatggc ggtggtcggc gtgatactgg tgaaggcgct ggtcaccgga ctgctgctgc
     9001 gtctcaacgg cgcccgcaag gcggtggctg ccgaggcggg gctgctgatg gcctcgccct
     9061 ccgagacaac cctgatcgtg cttgccgcgg cggcctcggc gcagctcatc gcgcgggagg
     9121 acgcggcctt ctggcagatc gtcaccgcga tcggcctgac gctgacgcca ttgctctcca
     9181 agctcggcca ggtggcggcc cggcgggtgg acctgggggc cgaaccggaa ttgccggcaa
     9241 taggcaccgc gaaggacgcc cccacggtcg tcatcatcgg cttcggccgg gtcgggcggc
     9301 tcgtggccga gatggtgaag acgcacggcc gcagctacct cgccgtcgat tccgatatcg
     9361 attcggtggc cgcctcgcgc cgcgacggct tcccggtggt gttcggcgac gtctcgcggc
     9421 ccgaactggt cgaccggctc aacctcggcc acgccgccgc cttggtgctg accatggacg
     9481 acccggtgct gacggttcgc cttacccgga aggtgcgcgc ctggtgcccc gatctcacca
     9541 tcgtcgcgag ggcgcgcgac gccgcccacg ccgccgagct ctaccgggcc ggcgccaccg
     9601 atgcggtgcc ggaaacgctc gaatcctcgc tccagctgtc ggaggcggtg ctggtggatc
     9661 tcggcattgc gatggggccg gtgatcgcct cgatccatga aaagcgcgcc gagctccgca
     9721 aggagatcat ggacctgggc gagatggacc gggagccgca gctccagcgc cggcggctgc
     9781 gcgacgcgct gaggcgctga tcagcggcgg agcgtcagct ggacgcgaag cccggcgtcg
     9841 ccctcgatcg tcttggtctc gcccggcgta agcgacaccg cctgggtgat ctggaccgtg
     9901 cgggtgccgc cttcgaggca ggcctgctgc gggatcggcc gctgccagct ggcgtcgacg
     9961 cggtagctgc ggccctgctg cggggtctcg ttcgcataga tgttgacggt cagctgccgg
    10021 cgctccgaac ggtcgtgggg tgtgtcgggc gggcattgaa aggccgaggc ctggttcaga
    10081 tgctgctggt agctcgccgc ctggttaggc gcgatccgga gcgtgccgtc ccacaacacc
    10141 gccccggcgg cctcgactcg gactgcgatt atctccggcg ccacacgagc ctccgccgga
    10201 accgcgttgg ggacgccgcc gacggccaca atcctcgttt gcgacgtgtg aagcgccagc
    10261 agcgccatga gctgaacgag catgatttcc cctctcgacg ggaaagctcg cacaataagg
    10321 tttagttttg ctcgcccggg atggttgcca agcgcttgct aaacggcttc cacctgctcg
    10381 ccgagcgcgc tgcggaccgc ggccaaagcc gcctcggcct tgccgccgtc gggaccgccg
    10441 ccctgggcca tgtcgggacg cccgccgccg ccttggccgc cgagctcggc gacggccgcg
    10501 cggaccagat cgaccgcgct caggcccggg gtaaggtcgt cggtgactcc gaccgccacg
    10561 gtggcgcggc cctcgttgac cgcgaccaaa gcgacgacac ccgagccgac gcgctgcttg
    10621 gcctcgtcga ccaggccgcg caggcccttc gggtcaaggc cttcgatgac ctggccgatg
    10681 aagccgtggc cgccgaccag ttcggggccc gccgcttcgg aggcgcggcc gccgccgagc
    10741 gcgagcgcct tcttggcgtc ggcgagctcg cgctcgaggc ggcggcgctc ttcgaccagg
    10801 gcggcgacgc gggcgggaac ctcgtccggg ctgctcttca gcacggcggc ggcggcgcgc
    10861 agcttctcgt cgcggccgtt gagataggcg cgggcggcgt cgccggtcag cgcctcgacg
    10921 cggcggatcc cggatgaggc cgcgccttcg ccgatcacct tgaacaggcc gatgtcgccg
    10981 agcgccgaga catgggtgcc gccgcacaat tcgaccgaat agcggccctc gtcagtgccc
    11041 atcgagacga cgcggacctc gtcgccatat ttctcgccga acaaggccat cgcgccctcg
    11101 gcgatcgcct cttcgggtgt catcagccgg gtcgtgaccg gattgttggc gcggacctgg
    11161 aagttgacgt cgtcctcgac ggtggcgaga tcggcggcgc tcatcgcgct cggatgggag
    11221 acgtcgaagc ggaggcggtc gggcgcgacc agcgagcctt tctgggcgac atgcttgccg
    11281 agccgctcgc gcagcgccgc gtggagcagg tgggtggcgg aatggttggc gcggatcgcc
    11341 gagcggcggc cgccgtcgat gcggagcttg accgggtcgc cgaccgccac ccgcccggcc
    11401 tcgacgacgg catgatggac gtggaggcgg ccgagctgct tcgacgtgtc ggtgacgcgg
    11461 gcgcgaaggc cgccgtcgct gacgatctcg ccggtgtcgc caacctgtcc gccggattcg
    11521 gcgtagaagg gcgtctggtt ggtgacgatc atgaccgcct cgctctcgcc tgcctgctcg
    11581 acgcgcgcgc cgtcgcggac gatcgcgacc accgcgccct cgccttcttc ggcggtgtag
    11641 ccggtgaatt cggtgctgcc cgcttcctcg gcgatgtcga accagaggtc gtccgacgcc
    11701 ttggcgcccg agcccttcca ggcggcgcgg gcggcggcct tctgctcggc catggcggcg
    11761 tcgaagccag cgcggtcgac gccgaagccc tgggcgcgca gcgcatcctc ggtgagatcg
    11821 tagggaaagc cgaaagtgtc gtagagccgg aaggcgacgt cgcccgccag cgtctcgccc
    11881 ggcgccatcg aagcggtggc ctcgtcgagc aaccgcagtc ccttgtcgag ggtctggcgg
    11941 aagcgcgtct cctcctggcg gagcgtcgcc tcgatcagcg gctgggcgcg gagcagttcg
    12001 gggtaagcgg cgcccatttc ggcgaccagc tccggcacga gccggtgcag cagcggctcg
    12061 gcggcgccga ggatatgggc gtggcgcatg gcgcggcgca tgatccggcg cagcacatag
    12121 cccctgccct cattggccgg cagcacgccg tccgcgacga ggaagcccga ggcgcgcagg
    12181 tgatcggcga tgacgcggtg gctggcctgc cgctcgccgg tggtggcggt gcgggtgagc
    12241 tcgcccgacg cggcgatcag cgccttgaag gtgtcggtgt cgtaattgtc gtggacgccc
    12301 tgaagcacgg ccgcgatccg ctccagcccc atgccggtgt cgatcgacgg ccgggggagc
    12361 tcgcggacga tctcattgtc ctcctgctca tattgcatga agacgaggtt ccagatctcg
    12421 acgaagcggt cgccgtcctc gtccggcgat cccggcgggc cgccggggat gtgattgcca
    12481 tggtcgtaga agatttcgga gcaggggccg caggggccgc tgtcgcccat cgcccagaaa
    12541 ttgtccttgg tcgggatgcg gatgatgcgc tgctcgggga gccccgccac cttgcgccag
    12601 agctcgaacg cctcgtcgtc ggtatgatag acggtggcgg tgagccggtc gggcgagagg
    12661 ccccattgct tcgtcagcag cgtccaggcg tggacgatcg cctgctcctt gaaataatcg
    12721 ccgaacgaga aattgccgag catctcgaag aaggtgtggt ggcgcgcggt gtagccgaca
    12781 ttgtcgagat cgttatgctt gccgccggcg cgcacgcatt tctggctcga cgcggccgtg
    12841 ctgtaggggc gcgtctcgag gccggtgaag acattcttga acggcaccat gccggcattg
    12901 acgaacatca gggtgggatc gttgtgcggc acgagcggcg ccgacggcac catccggtgc
    12961 ccggcggccc cgaaatgatc gaggaaggag cggcgaatgt cgttcgtcga ggtcatgccg
    13021 gcgatgtagg cgaggcgcgg agtgcgggca acccggcccg ccgcgccctc caggcctagc
    13081 tgctacagac cgacggggac gggctcggct tcggcctgcg cctcggcgac cggctccacc
    13141 acgacctcga gcgcgcgctc gtgacggcgc atctcctcct cgtcgaccgg atagcgcgaa
    13201 taggcctcga tcagccgctg ctcctcgctc cgcgccgcgg cgaactgcgg cagctgctgc
    13261 accgcctgct ggaccgcggg ctcttcgtca gaccaggagc gttcgagttg ggcgtccacc
    13321 gccggatcgc ggcgcggaaa atattcctcg ggataggtgc ggcagccgac gcagccccgg
    13381 ccgagcgccg cgccatattc ggtcgccgcc acttcccgcg gccgcagcgc gtcgaaatcg
    13441 tcgccgcggt tcggggtcag cgccgcatag gagcgggtcg cttcgggaag agaaaaatag
    13501 agcgggttga tctcgtcgac ggccgcgcgg ccgagataga cgccgagccc ggtgccggcg
    13561 agcgccagca tcgccacggt aggaagcgtc aacggatgcg ccatcgtacg gccctccaaa
    13621 acaggcgacc accttaatcg gacggccgca atgcccaaac tctcaatgta ccggctgcgt
    13681 tccaaaaccg ggcgttctta tccggccgtt catcgaatcg tcctcttgca ctcggccgca
    13741 aacaggcgga cggtggccga agcatggcgc ggacgcgctc ttcgcggacc gccccggacg
    13801 gacgaaggag aggttcatgc ccgaaagatc ggttggcacc agaatggtcg cgctcgtcgg
    13861 ccccggcggc gcaggcaaga cgagcctcgc cgaggctttg ctgttcgcgg cggggacgat
    13921 cgaccgccag ggcagcgtcg atggccgcag cagcgtcggc gatgccagcc ccgaggcgcg
    13981 ggcgcgctgc gggtcgaccg agatcaacct gatgcggctc gaattcctcg gcgaccggtt
    14041 cgcgatcgtc gattgtcccg gctcggtcgg cttcgccgcc gatggcgcga tggcggtggc
    14101 cgcggccgac atcgcgatcg tggtcgtcga cccggacccc gcccgcgcct tgctcgccga
    14161 accggcgctg cggcagctcg aggcgctcgg aatcccccac gccattttcg tcaacaagat
    14221 cgaccaggcg cgcggcagca tccacgacct gctcgaagcg ctgcagccga tgagcaattc
    14281 gccgctgatc gcgcggcaga tcccgatccg gggcaaggat gcgggcggcg agaagatcac
    14341 cggcttcgtc gacctcgcgc tcgaacgcgc cttccactac cgcgcgggca agccgtccga
    14401 gcggaccgag atccccgccg acctcgccga gcgcgaggcg ctcgaacgtt atcacatgct
    14461 cgagcagctc gccgaccatg acgatgcgtt gctcgaacaa ttgctgatgg acgagacgcc
    14521 ggacctcccc accgtgctcg ccgacctcgc ccgggagacg cgcgagaatc tcggcgtgcc
    14581 ggtactgttc ggctcggcgc tcaacggctt cggcatccgc cggctgctga aggcgcttcg
    14641 ccacgaggcc cctgccccgg ccgacgcggc cgcgcggctc ggcgccagcg acggctgcgc
    14701 cttcgtgttc aagatctcgc atggcggcgc gatcggccgg ctggcgctgg cccgggtgtt
    14761 cggcggcgcg ctcagcgagg gcacggagct caaggggccg gacggctcgg ccgtgcgggt
    14821 cggcaccttg ttcgcgctcc agggcgagaa gaccgccaag ctcgtcaagg ccgaaatggg
    14881 tgaagtggtc gccatcgcca aggccgaatc ggtgcacgcc ggcgggtggc tgagcctcgg
    14941 caagtccgca ccggcgcccg cggtcgagct tccggccagc aattacgcgc tggcaatcgt
    15001 cacccgcgac cgcaaggacg acgtccggct gtcgacggcg ctccacaagc tggtcgagga
    15061 ggatccgtcg ctgagctggg agcaggacga ggcgcttcac gagacgcggc tcaagggggt
    15121 cagcgacgag catctcaacg tcaccatcgc ccggctcaag cggcgctacg gcgtggcggt
    15181 ggattcgaag ccgccgacga tcggctacaa ggaatcgatc cgccgccagg tgacgcagcg
    15241 cggccgccac aagaagcagt cgggcggcca cggccagttc ggcgatgccg tgatcgaagt
    15301 gaagccgctg ccgcgcggcg gtggcttcgc gttcgacgag cggatccatg gcggcgcggt
    15361 gccgcggcaa tatataccgg cggtcgagca gggcgtgcgc gacgcaatgc tgaaagggcc
    15421 gctcggcttt cccgtggtcg acgtcgcggt gacgctcacc gacggcagct atcacagcgt
    15481 cgacagctcg gagctcgcct tccgcacggc cgggcggatc gcgatgagcg aggcgctcgc
    15541 cgccgcccag ccgcatttgc tggagccggt ccaccgggtg acggttgtcg cgccgggctc
    15601 ggcgacgtcc aaggtgacct ccgccatttc gagccggcgc gggcagatgc tgggcatgga
    15661 gccgcgcgag ggctggtcgc gctgggacaa ggtcgaggcg ctgatgcccg aatcggagct
    15721 gcaggggctc gacgccgagc tgcgctcgct gagccagggg ctggcgagct acacggccga
    15781 gttcgaccac ctcgccgaac tcaacggcaa gctcgccgaa gaggtcgtgc agaaggtgct
    15841 cgagccggcc tgatctagct cggatgagcc tcgaccgtcg cggcgtccgc aatgggcgcc
    15901 gcggcggggg cgtcctcctc gccgcgtgag atgacgacgg cggcggcgag gtcgccggtc
    15961 acgttcaggg tcgtgcggca catgtcgagg aagcggtcga cgccgagcac caggccgatg
    16021 ccctcgggcg gcaccccgac catgccgagg atcaaggcga cgacgggcag cgagccggcc
    16081 ggcacgccgg cggtgccgat gccgccgagg atgcagacca gcagcaccat cacctgctgg
    16141 gcgagggtca gctcgacgcc gaagaactgg gcgaggaaga tgaccgtcac gccctcgaac
    16201 atggcggtgc cgttctggtt ggcggtggcg ccgatggtga ggacgaagcg cgacacgcgc
    16261 ggcggcagat ggagcttttc gtcggcgacc ctgagcgcgg tcggcagggt ggcgttggac
    16321 gaggcggtcg agaaggccat caccatcgcc tcctggctct ggcggaagaa ccagagcggg
    16381 gacttgccgc cgaggaagcg gatggcgagc gaatagacga cgatcatgtg gatgccgagc
    16441 gcgagcagca ccacgccgac ataggcgccg aggcggagca gcaggtccca gccgaacacc
    16501 gcggcgaggt tgaacatgaa gcaggcgatc gcgatcggag ccagccggat gacgatgccg
    16561 atgaggcgca tggcgacgtg gaagatgccc tcgatcgcct cctggaggcg ctgggcggag
    16621 ggcgagttca cgagcagcag gccgatgccg aacaccaagg cgaagaacat gacggcgagg
    16681 atgtcgttgt cggcagcggc ggcgacgatg ttgttgggga tgatgttgag gaaggcggcg
    16741 acgcccgacg gggtttcggc gctcttcgac acgatcgcgc ccgccccttc acgggcatcc
    16801 tcgagcaggc tctgggcgag caccggatcg acgccgccgc cgggattgaa gatattgacc
    16861 acagccaagg cgatcacgac cgcgaccgcg gatatgccga tggtgaagat cagcgtcttg
    16921 atcccgaccc gcttcagcga tcggatgtcg cccatttcgg caatgccgac gacgagggcg
    16981 gaaaagagca gagggataac cagcatgaac agcaggcgca ggaagatctg gccgatcggg
    17041 ccggtgacat aagtggtgac cgcctcgacc cagggcgcgt ccggctgggt gacgtggacg
    17101 agcagccccg cggaaaggcc gaccacgaag ccgatcagca tcttcatctg cagcggcacg
    17161 ccgcggtggg tggtttccat attgtcgccc ctaagctgcg cgccttgccc ggtcacgtgc
    17221 gagcaccgct ccgaaatcgc gggggccggg ggcaaaagtc aaccgatttg aaggggagcc
    17281 ggggacagcg gcggctttca cccgaaggga cggtcgatcg agggcggcgg cgtcgagaac
    17341 cagtgcgggc cttcgggggt catgtagaag cagtcctcga ttcggacgcc gaagcggccg
    17401 ggaaggtaga ggccgggctc gttggaaaag cacatgccgg gggcgagcgg cgtggtttcg
    17461 ccgtggacga ggttgaccgg ctcgtgccct tccatgccga tgccgtggcc ggtgcggtgg
    17521 gacaggccgg gaagcttgta gccggggccg tagccccatt cctgatattg cgcgcgcacc
    17581 gcatcgtcga cgctgccggc gggagcgccg gggcgggcgg cagcgaaggc gatctgctgg
    17641 ccgcgatgga cctggtccca aaccttgcgc tgctcacccg tcgcctttcc tccgaacacg
    17701 aaggtgcgcg agacgtccga ttggtaccca tggacggtgc agccgcaatc catcagcacc
    17761 acctcccctt cgcgcacctg ctgcggcttg ccgctgccgt gcggataggc gctcgcttcg
    17821 ccgagcaggg cgagcgcgaa ttcggggctg ccgcccagcg cgaccgtggc ctcgttcatg
    17881 atcgcgctga tctgggccgg agtcatgccg gcggcgatgc gcggcgcggt gtgggcgtag
    17941 gcggcgagcg tcacgtcggt cgccttttgc atcagcgcga gctcggccgg cgacttcacc
    18001 atccgcagcg cccgcaccac cggggcgccg ctgcgtatct cgaccgacgg cagcgccttg
    18061 cgcaggccat cgaccgcgaa gaagcgcacc gtctcctcca taccgaccgg gcgggtggcg
    18121 agcttgcgct cgcgcagcgc ggcggcgacg agggcgagtg gatcctcatg ctcggtccag
    18181 gtgcgcaccg ccgccgggac ggcgaggctc tcgcgcaccg acggctcttc gaagaagggc
    18241 gtgaacacga tcggctcgcc ctcggccggg atcagcgcgg cggtgagacg ttcgctgcgc
    18301 caccattgga cgccggtgaa ataatcgagc gacgcgcccg gctcgatcag caacgcgccg
    18361 aggccttggg cgcgcatcag ccgctgggcg ccggcgaggc gctggagccg ttcgtcgcgt
    18421 ccgatcggct tcgcatcgcc ggtgatcgga gcgagggcgc tggggactgg agcggcggcc
    18481 atgatccggg atcctccgat gagcgcgccg ccggcggcga gcgcggatag agtgaaggcg
    18541 cggcggccga gctcaggcat aggcataagg cccgcctttc tggagcgcgg cggcgtaagc
    18601 gggccgggcg tggatgcgct ccagccagtc gatcaggtgc ggccggcttt cgtcgaggcc
    18661 gccgcggctg cgcgaggctt cgagcgggaa gctcatcatg atgtcggcgg cgctgaattc
    18721 gtcgccggcg aaccagtcac gctcggcgag ctcggcctcg agccagtcga gatggtcgtc
    18781 gagcatcttc tgaatcggcg cgcgcgcggg cttgccgagg atgccgagcc gcccgaccac
    18841 cagcaaggcg agcaaaggcg gcatcatcga gccttcggca taatggagga agtggcggta
    18901 gcgcagcacg ccctcgcgcc gcgccggagg gccgagcttc ccgtccccct tttcgaccag
    18961 atattcgacg atcgcgccgg tttcggcgag gacccgccgc tcgcccgcct ccgagcccga
    19021 gcccgcatcc tcgatcaccg gcgacttgcc gagcgggtgg acacggcgaa gctcgggcgg
    19081 ggcgagcatc gtcttcgggt cgcgctcgta gcggcggact tcgtacggaa ggccgagctc
    19141 ttcgagcagc cacaggatgc gttgcgaacg gctgttctcg agatggtgga cgatgatcat
    19201 cggctggtcc gggcgcagag cgccgcgacc tccttgacga gcttggcggc gagcacggcg
    19261 gtgactccgt tgagatcccg gctgaaatta tattcgacga tgtcggcgcc gacgatggga
    19321 gcctcgaccc ggtcgagcac ggcgagcacg tcgcggacgg agaggccgcc gggctcgtga
    19381 tgcgagacgc cgggggcgaa ggcgggatcg aagccgtcca tgtcgatgct gatgtagagc
    19441 ggacccgacg ggatcgggac ccgatcgggc gcgaaatggc gcatctcgac gacctcgacg
    19501 ccgaagcgct cggcctgctc gcggcagtgg cggttcaagg tgcggatgcc gacctggacg
    19561 aggcggcggg cgaggccgcg ctccaggatg cgggcgaagg gcgaggcgtg ggagtgcggg
    19621 tcgccttcga aatcgtcgta gaggtcgggg tgggcgtcga agtggaggat gtcgaccggg
    19681 ccgtggacgg cggccacgcc ctcgacggcg gggaagctga cataatgatc gccgccgagg
    19741 cagagcggga ccgcgccttg cgaacaggcc gccgcggtcg catcgcggat cagcgcatgg
    19801 tcgccctcgg cctcggcgag cgggaggtcg ccgccatcct cgagccatat gtcggtgccg
    19861 agctcgagcc ccgattcggc gttttggttg ccatgatcgg aggcgagcgc ggcgcggatc
    19921 agtggcggcg cggcggcggc gcccctgagg aaggaggaat ggctgtcggt cggaaggccg
    19981 atcagccgca gccggggccg tgtgtcgagg gtcatgcgcg ccccttgcgg tgaggcccgc
    20041 ctgttagggc gcacgattat ggcttgtcca gccgcgcccg ccgcaccaga tggcccgctt
    20101 ggccgagcgg cagcgaggaa agtctgaatg agcgatcggg aagccgcgcc gccggcggcg
    20161 gattggcgcg ggccggggct gcgcgagttc gtggccctgg tcgcggcctt gatggcgtcg
    20221 aatgcgctgg cgatcgacgc catgctgccg gccctgcccg cgatcggcga ggcgctcggc
    20281 gtggccgagg acaatcggcg ccagctggtc atcaccgcct atctactcgg cttcggggtc
    20341 gcgcagctcg cttacgggcc gctgtcggat cgttatgggc gcaagcggct gctggtcggc
    20401 gcattgctct tctacggcgt gttcgcggcg ctggcgggga ttgccggcag cttcaccttg
    20461 ctgctcggcg cgcgggcgct gcagggggtg gcggcggcgg cgacgcgggt gctggtcgtc
    20521 tcggtggtcc gcgaccgcta ccaaggctcg gcgatggcgc agatcatttc gacggtgatg
    20581 atcgtgttca tgatcgtgcc ggtcctggcg ccgtcgctcg gccagggcgt gctggcggtg
    20641 gcgagctggc ggcacatttt catcttcctc gccgcttatg gcgtgatcct cgcgctctgg
    20701 acagggctcc ggcttcccga gacgctacgg cccgagcacc ggcgggcgct ttccgtggcg
    20761 cgcatcgccg aggcggcctg gatgacgctc ggcaaccgcc agtcgatcgg caacacgctc
    20821 gcccagaccc tggtgatggg ggcgctgttc gcgttcatca actcgatcca gcagatcgtg
    20881 ttcgacgtgt tccggcggcc cgagctgatc ggcctcgtct tcgcctgcat cgccggccca
    20941 atggcgctga gctcctacgc caattccaag ctggtgatgc gctggggctc gaagcggctg
    21001 ctgctgctcg cgctcgccgc ctttaccgcc gtctcggcgc tgcacctcgc ggtcgcggcg
    21061 gcggcgggcg aggcaatctg gacgttcgtc atcctgcagg cggcgacgat ggccttcttc
    21121 ggcctgatcg gcgccaatgc cggcgcgctg gcgatggagc cgctcggcca catcgccggc
    21181 accgcctcct cgctccaagg cgtgatcacg acggtgggcg gggcgctgat cgggctggcg
    21241 atcggccaga gcttcaacgg caccaccctc cccttcctcg ccggcttcgc aatgtgcggg
    21301 atggccgcgc tggcgaccgc cttctgggcc aaccgcccgc ccagcctcga ttcgcgcgac
    21361 cgggcggaag tggaagtgca ggagaccgcg gcgcggccgg gctgaagcgc ttatcttgtc
    21421 aaacttgtca cgaaagcgac cttcggcgcg gcgctaaggt gacgaagttt acactcgcag
    21481 cggcgcgggc tcaaccggct tggccgagcg cttttaccac gaactggccg ccgaggcgca
    21541 gcccgaccgg gtccacgctg tggccgaggc cgctcgagac atgggtggtc acctcgacgc
    21601 ctaggcgctg cagatcgctc ttggcggcgt ggagggcggc caccggcacg atcggatcgg
    21661 ccgagccgtg gatcagaagg accgggggct tgctccggag gtgatgggag agttcggcgg
    21721 cgccggtcag catgcccgaa tagccgagga tgcacgccgc cggccgcgcc cgcctgaggc
    21781 cgacgtgaag cgccatcatc gtgccctggc tgaacccgac cagcgcgaga tcggcctccg
    21841 tcaggccata ttggtccagc ttccggtcga tgaaggcatc gagcgccggc gccgcgcttt
    21901 gggcgccggc cgccagcgcc tgcggcgtaa aggcgttcag cccccaccat tgataggcgc
    21961 cgcccatccc catcgtctgc ggcgcgttgg gcgcgaggaa caatgcctcg ggcagcgcct
    22021 ggcgccagtg cggcgcgagc gagatcaggt ccgccccgtt cgagccgtag ccatggagca
    22081 gcagcacgat ctggcgcggc ttggcgccgg agagcggctg caggctcgag ccgttgacga
    22141 tcttggacag agtcgaactc gcttagctga gaaggcggca gttttcgaag gcggcggacc
    22201 ggtagcgggt cgcgggatca gccgcaacgg gcctccgcga acgtaaccag ccgccgcttc
    22261 tgctcgtccc acagcaccag ccgtaccgcg ccgatgcttt cgcgaaacgt tacccggctg
    22321 atccgggcca gcatcgggac ttcacgcagg acctcgggca gccggtagaa gggaatgcgg
    22381 ctggcgagat ggtggacgtg gtggacgccg atattggcgc tgaaccagcg caggaccggc
    22441 ggcagatcgt aatgcgagct gccgtgcagc gccgccgcgt ggaacgacca atcctcgccg
    22501 cggtcccaat gggtctgctc gaactggtgc tgcacataga agaaccacac gcccagggac
    22561 gccgcgacca tggtgatcgg caggtggacg agcaggaaca gcttccagcc catcgcccag
    22621 atcaaggcgc cggccacggc cgcgaagctg gcattggtgc cgagcgcgct gagccacggc
    22681 cgccagccgc gcttcatcat gccgatcgga agccggtgcc gcagcaggaa aagataagcg
    22741 gggcccaggc cgaacaggac cagcgggtgc cggtagaccc ggtaccagaa gcgccccgcg
    22801 cggctgagcg cgttgaattc cgccacggtg agcgtgtcga cgtccccgac gccgcgggcg
    22861 tcgagattgc cggtgctggc atggtggatg gcatgcgagc ggcgccagta atcgtagggc
    22921 gtcagcgtaa gcacgccgag gatgcgcccg agccagtcgt tgctcgcctg gcgccggaaa
    22981 aaggcgccgt ggccgcaatc gtgctggatc aggaagagcc tcagcaggaa ggcgccggcc
    23041 ggcaccgtca gcagcaggcc ggccatatag cctgcgtcga gcgcggccca ggtcagcagc
    23101 cacagcagcg tgaagggcgc cgcggtgatg gcgagctcga acaggcttcg ggccgtccgg
    23161 gactgccgga agggaccgat ctcgcccatc aaggcgcggg gatcgatggc ggtgttggtg
    23221 gagaggggag cgtgcaagta ggggctttca atcggggggt cggagccggg cgaggccccg
    23281 gggagcaatg gccgccggcg gaatgccgtg gagccggtcg cggcagacac gatccatgtt
    23341 taacctgagc ttaccgctcc ttggcaggtg tggctgccaa gtcaacgccc acgagacaac
    23401 gggaaatcgc atcgaggtgg gcattggggg accgggagcc tgatcattgg cgcttcgtga
    23461 acggacaggc ggagccgttc gcgacgggcg ggcggcgatt cacggaaacg cgctggcacg
    23521 gcggcgggcg agcgtcgagg cccagggctt cgcagccatc actaaggatc gccttcatga
    23581 aaaccaccct cctcgcggcg ttgatcgccg cgcttgccgc tcccgccggg gcccatgcgg
    23641 ccccagctgg atcctcccgc ctgaccctta ccttcgaaac cggcgccgcg accggcgcgg
    23701 tgctggtcgc gctgttcgat tcgaaggccg cctatgatgg cggcgctccg gtccgccagg
    23761 cgcgggtcga cgtcgccaaa ggcgaacgga tcgcgatctt cgctaatctc gcgtcgggcg
    23821 actatgcgat gaaggcattc cacgacctcg acggcgatgg ccggatgacg accaacccgt
    23881 tcggccgtcc gatcgaaccc ttcgcctttt ccaacaatgc ccgcggcaac atgggtcccg
    23941 ccgcctggga tcgcgcccgg ctgaaggtcg ccgccgacgt ctcccagatc gtcgaaatcc
    24001 gctgatgcag ctccaggaca cccaactggt cttttcacgc cgcgccatga tgctcggcgc
    24061 cgcctcactg gtggcgacgc cggacatgag ctgggccgcc aaggcgctcg cgatcgcgtc
    24121 cgactggtcg ctcgccaccg ccgatgtcga ggccgacctg cagccgcgcg ccctgcggct
    24181 cgccagcggc cgcgccccca aggggctcga aggctcgctc tatcgcaacg gcccggccaa
    24241 gttccgccgg ccggggcgct ccgccggcca ctggttcgac ggggacggac tgatccgccg
    24301 cttccgcatc gccgacgggg aggcgagcct ggccgcccgc ttcgccgaca ccccgaaacg
    24361 aaggctggag acgcggctgg gcgcgatggt catgcccggc ttcggatcgg cgtccgatcc
    24421 gcgcgccgag ctgcgcgggc cggacgatgc caatgcggcc aacacttcgg tgctgccggt
    24481 gggcgacaag gtgtgggcgc tgtgggaggg tggctcgccc atggccctgg acggcgccac
    24541 gctggcgagc gaaggcttcg tcaccttgcg gccggacctc aaggcaatgc ccttctcggc
    24601 gcatccgcgg atcgagccgg acgggcgcgt ctggaatctc ggcatcaacg gcaagcaggc
    24661 catggtgtgg cggctgtcgg cggccggcgc gctcgaagcc gccgagctca tcgagctccc
    24721 gcgcgccagc tatttgcacg atttcaccgc cactgcccgg cacttggtgg tcgtgctgca
    24781 gccatggatc caccaccgcg aggtcttccc gctggcgcgg ggctatagct ggcggcccga
    24841 gaccggcacc caggtgctga tcatcgacaa ggcggacttg tccagacgcc ggatcgtcga
    24901 gctgccgccg ttcggtttct tccacctcgg cgacgcctgg gaggaggcgg acggcacgat
    24961 ccgcttcgac gtctgcgcct atcccgacat gcggttcgcc gcctcgggcg cgagcgatct
    25021 cctcgacagc caatatgacg gcgggggctg gtccgagatg gtgctcgcaa tgctgcctct
    25081 ccgcggcccg ggccgcctgg agcgaacgag cgtcggggcg gagttcccga agggcgatgc
    25141 gaggagggcc gggctgccgc gccggttcag cgtccatacc gccggcgata cgccgggccg
    25201 gccgatggcg accggcgtcg gcgtgaccga ctggcggagc ggccgcaccc ggaccttcga
    25261 cttcggcccc aatcacgtgg tcgacgagat ggtgccggtc cccaagcccg gctcgaccgc
    25321 cgaggaccag gcctggctgg tcggacccac catcaatctg aaagcgggcg tgaccgagct
    25381 gcacgtgctg gacatggcgc gggtcgagga cgggccgatc gccacctggg cggccgacgt
    25441 cgcgcttccg gcctcgtttc acgggcgctg gcagtcggcg tgaccgatca gtaccagccc
    25501 gcctcccgca gcgcgcgggc gtagcggcgg ctgaccgggg cggtgagccc tccgtccagc
    25561 gtcagcgtgg cgcggccgtc gccgcgttgg gcgtcccgca ctgcgtcccg cgcgacccac
    25621 cagctgcggt gggtctgggc gccttccagc ccctccagct catccagcgc gtccgacagc
    25681 cgcatcagga tgaggtgtga cccgtcggcg gtgtgcacgc gcagataatg gtcctccgcc
    25741 tcgaccgcga tgagccggct gccgcgcagg cggagcggga ggcgctcgat gaatttgacc
    25801 ggcttgccgg cgtcggcggc ggcgtgggtc tggacggggg ccgcccggcc cagcagcacg
    25861 accaaggcgc agaccgccgc cgtcaccgtc accaccggca ccagcaccag cggcagcgag
    25921 cggaccggat agagctcgcc gtggaagaac agcccggtcg ccgcccagac gaacaggcac
    25981 ataggcccgg cgacgatggc ggtgaggacc ccgatgtgca gccacggccg ctcctccagc
    26041 tccatccgcc gcgccagcaa tcgctggcac agcatgcccc acgccgagcc caccgccatt
    26101 gccatgaacc agtaggtcag ccgctcatgg aacagctcgg cgccggtgcc gaaagcgccg
    26161 ctcaccgcca ggacgagagc ggcggcggcg gccacgccca ggttgcggac gaggtcgcgc
    26221 cgctcggggg tcatggggcg gggctcgaaa cccggagagc cgcggcgggc aaatatcgaa
    26281 gcatcgaacc gcctcctgct cgccacgagg tcgcatgccc gtgcgccgcg gacaagcgcc
    26341 ggcgtccgag gatggcttca ccgatgaccg cgccggctct aaacagtgtt gatgaacttc
    26401 gaagaccagc tccagcgtta tttcggcacg gccgacatcg cttccatctc gccggcggcg
    26461 ctgggggcgg cggtcgagcg gatgcgggtc gacctcggcc tcgagacgga ccgggggcgg
    26521 cgcttcgcct tgtgggcctt gctgcacatg ctcggttccg cgccggacct cgatgtcgcc
    26581 ttccccgatc cggccgaccg cgacgccgcg cgcaacttca tggagctgtc cgagcaggcg
    26641 ccgccgggag ccgctggcga ttgatcactc tccgcacgca gaatcccgct tacgagaaaa
    26701 cgctttcatg ctaagccctt agccgctggt ctggggtagt gtgcgccggc ccatcgcgtt
    26761 ggaaatttgt ggcgtggcga cttacaacgg cggcaaatat aacgattggt atgtcggcac
    26821 cgactccgcc gacacggccc acggcaacgc cggcgacgac cggatcgaga cgcgcggcgg
    26881 caacgatcgc ctgtacggcg gctcgggcag cgaccagctt tacgcgggga tcggggacga
    26941 tctgctcgac ggcggcaccg gcgccgatta tctcgacggc gacgacggca acgacaccta
    27001 tgtggtcgac catgtcgacg accgcatcgt cgagcgcagc ggcatcgaca ccgtccaggc
    27061 cgccttccat tacgtcctcg cggccgagct cgagaacctg acgctgaccg gcaccgccgc
    27121 gctcggcggc ttcggcaacg acttcgccaa cgtcatcgcg ggcaacggcg ccgccaaccg
    27181 gctggacggc ggcctcggcg acgacacgtt gaccggcggc gccggcgccg acagcttcgt
    27241 gttcagcacc gcgctcaatg tggggccgaa tttggaccgc atcaccgact tctcggtcgc
    27301 cgacgacacc atcctgctcg accggtcggt cttcacgggc cttggggcca atggcgcgat
    27361 cgccgcgtcg gcgttcgtca acggcgccac cgccgcggac ggcgacgacc gcatcctcta
    27421 cgaccgcgcc accggcaaca tattctacga ttccgacggc agcggcgccg gcgtgaagtt
    27481 cttgttcgcg cgcgtcaccg ccggcaccgc cctcaccagc gccgatttcg tcgcgaccgg
    27541 cggcgctgcc ggcacccttt ataccggcga cgcggccgac aatctgttct cgggcagcgg
    27601 cggcgacgac caggccagcg gccatggcgg caacgacctg ctcctcggcg gcggcggcag
    27661 cgaccgcttc gacggcggcg agggcaccga ccgcatttac ggcggcgacg gcggcgacca
    27721 tctcgatggc ggcaccgggt ccgactacct ccacggcggc cgcggcgacg acacttatgt
    27781 ggtggacttc gaatcggtcg attcgcgcta tccgaccgac cgagtcgtcg aggccgccaa
    27841 cgaaggcgtc gacacggtgc tgagctcgct ccgctcctac gccctcacct ccttcgttga
    27901 gaatctgacg ctgaccggca ccgcctatca aggcatcggc aacgcgctcg acaacgtcat
    27961 caccggcacg tcggccaaca accgcctgtc cggcgagcgc ggcgccgacc gcaccatcgg
    28021 cggcgcgggc gacgatttct actgggtcga caatgcgggc gacgcggtcg tcgagctggc
    28081 cggcgaaggg gtcgacgccg tgcaaagctc ggtcacgcac cggctcgccg accatgtcga
    28141 gaacctttcg ctggtgttcc aggacagccc ttatggcgcg gtcagggact ttgacggcta
    28201 cggcaacgcg ctcgcgaacc ggatcagcgg cagcgtctac aacaatctga tcgacggcgg
    28261 gctcggcaac gacacgctga ccggcggcgc gggcgcggac cggttcctgt tcagcacggc
    28321 gctcaatgcc gagaccaacg ccgacctcat caccgatttc tcggtaggct acgacatgat
    28381 tgtgctcgac cgggcggtgt tcaccgcgat cggcgccgaa gggccgctga gcgcctccgc
    28441 cttcgtcaac ggcgtcaccg ccctcgatgc cgacgatcgc atcctttacg cgaaagagac
    28501 cggctacatc ttctacgacg ccgatggcag cggcgccggc gcgaagatat tgttcgccaa
    28561 ggtcaccgcg ggcacggcgc tcagcaacgt ccacttctcc gcggtcggcg gcgagccgcc
    28621 ggtctatggc ggcaccggcc cgacctacgt gggcgatgcc ggcgacaata tgttcgtcgg
    28681 cggcaacgag gcggacgagg ctgaaggcaa cggcggcaac gaccggctgc agggcaatgg
    28741 cggcgacgac atcctcgacg gcggcgccgc caacgacacg ctcgaaggcg ggattggcgc
    28801 ggacacgctg cgcggcggcg acggcagcga ccggctctcc tcctcggtca cccccaacac
    28861 ctatggcgac ggctggcagc cggcctcgcc cgacaccggc gcggaggccg acactgtgtt
    28921 cggcggcgcc ggggacgacc agatcttcgc cggctatggc gacagcgtcg acggcggcgc
    28981 cgacaccgac cggctctata tcagcttcca gggcgcgacg gcgggcgtcc aggccgactt
    29041 cagcaagctc tattcgggcg ggtcgatgac gatcggcggc gcgacgataa gcggcatcga
    29101 agccgtcatc cacgtcgagg gcagcaattt cgacgacttc atcgccggca cgtcgggcga
    29161 gttcaacggc accgtctacg gccgcggcgg caacgaccat atcctcgccg ctttctcgag
    29221 cgggctgatc tacggcggcg acggcgacga cctcatcgat tccagcgcca ccgtcacgtc
    29281 gtacagccat tatggcgagg ccgggaacga ccggctgatc ggcggcgagc tcaccaacaa
    29341 gctgtacggc ggcgagggca acgacttcct cgatggcggg cggcaggccg attatctgtt
    29401 cggcggcgcc ggcgacgata cctacgtagt cgatgtcggc ggcagccatc ccgaggatgc
    29461 ggtgcgggac caggtttccg aagcggccgg cgaaggcacc gacacggtgc tcgcctccgc
    29521 gacttactgg ctcgccgcca atgtcgagaa tctgaccctc accggcgcct cggcgatcgg
    29581 cgcccacggc aacggcctcg acaatgtcct caccggcaac gcggccgcca accggatgta
    29641 cggagccggc ggcgccgacc gcatgagcgg cggcgcgggc gacgacgatt atcaggtcga
    29701 gaatgccggc gacctagtcg tcgaagcggc cggcgaaggc atcgacgcgg tgcagagcga
    29761 gatcagctac acgctgaccg acaaggtcga gaccctgctg ctggtcggca cccggacgag
    29821 catgggtacc tatgtcgacc aggcgatcga cggcaccggc aacgcgctcg acaaccagct
    29881 ttacggcaac agcgccagca acatcctcga cggcggcgcc ggcgcggacc tggttgtcgg
    29941 cggcgccggc aacgaccggc tggccggcgg ctccggcgat gaccggctcg aaggcggcga
    30001 cggcaacgac atctacacgg tcgccagcgc cgacgatttg ctggtcgagc ttgccggcca
    30061 gggcagcgac acggcgcgcg cctcgatcag ctacacgctc ggcgacactg tcgagcacct
    30121 cgcccttctc ggacgggcgg cgctcgacgg caccggcaac cggctcaaca accggctgac
    30181 cggcaatgtt ggcgacaacc gcctcgacgg cggagccgcc aacgacgtgc tcgccggcgg
    30241 gctcggcaac gacatcttga tcggcggcga cggcggcgac cggctgaccg gcggcaccgg
    30301 gcaggacctc ctcaccggcg gcctcggcgc ggacattttc ctgttcgtcg aggaggccga
    30361 ctcgccggtc gccgccgggg acgcgatcgc cgatttcgcg caggggctgg acaagatcga
    30421 cctcaccggg atcgacgccg acgccctcgc cgccggcgac caggccttca ccttcatcgg
    30481 caccgaggcc tttacgggcg gcggcgccgc cggagcgggg caactgcgct acgaacaggt
    30541 ggacggccgg acgctgatct cgggcgatgt cgacggcgac ggggccgccg acttcctgat
    30601 ccggctggac agcccggcgc cgctccaggc caccgacttc ctgctctagc ggcgagggcg
    30661 gctgggaggt tcgtgccgct gtcccgagcg cagccaaccg tcattccagc gtaagctgga
    30721 atctcccttc ttcttcgcga cccgcaagat tccagcttgc gctggaatga cgggccgagc
    30781 cggtgcactt cccgcaaaaa aaatccctgc gaccccctgc gggtccggag cggcggcggc
    30841 gtctgggcat gagacactgc tcatgggggt ccgccggcac atgtccgtat ccgagttttc
    30901 gcgccgccgc gctttcgccg cctcggtcag cctcgcggcg ctggccatgg cttccgccgc
    30961 cgctccggcg cttgcccagg aggcctgcgg gccggtcgcc gaaggcgtcg taacctgcga
    31021 cgaggccgat gcgcccttcc cgcaaggcat cgcttattcg acttccgagg atctgacggt
    31081 ggtggtcgag gacggcctca ccgtcgcgcc ggacgcaggc gttgcgggcg tcatcctgtc
    31141 ttcggaaggt tcggtcacgc tccaggcggg cggcgctgac gtcgtcgcca atggcgccag
    31201 cgcgatcttc gcggccggca gcgacgatat gacgatcgtg gtcgacgacg ccgccgccac
    31261 cggcgactat ggccgcggca tcgacacctt caccttctgg ggcgacaccg agatcgtcgc
    31321 cgacagcgtc tccacctcag gccgcgacgc actcggcatc accgcg
//
LOCUS       DASVRK010000134         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_23951, whole genome shotgun sequence.
ACCESSION   DASVRK010000134 DASVRK010000000
VERSION     DASVRK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23951"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VF589_05175"
     CDS             <1..1053
                     /locus_tag="VF589_05175"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEX8257003.1"
                     /translation="LPVALPGRKYHLRTIVVSADLLARLIATGTPPDLVAEVARLEAR
                     AAMVEEALEGRRAADRNRQQNRRGARASRDITLGSVTTRDVTDAPPPDKEKSPRPSKE
                     INLPRPIDEADASSTGPKNPVSPDRPKKGGSERPDRQQPDRQPGDRQPSERQRKGARI
                     PDDWTPPLIDDLPPMAQALASQWPAGAYAAQGELFAAYWRGEGGRQGARKLDWTQTWC
                     NWIGRVTGQVLRDAKAGVRFAADAPARQRTAAEIERDERLARELDALHRAETAEARAI
                     RERIRRDAGARTYDGWVKPCAIAIEGDDIIVSSPSEFVSGWLEQHFAATFTGVGGTVL
                     GRPVRQVLFRVVKPPG"
     gene            1100..>1967
                     /locus_tag="VF589_05180"
     CDS             1100..>1967
                     /locus_tag="VF589_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257004.1"
                     /translation="MADDAENVRGWARRRLAALEGAERPEREARGPSFFKRLALREAE
                     RVENILAADKIGAERKTGPSRRQRARMVVPTDERIGQASAGGEQLEWVNPAEIDSAEQ
                     AIGLTRRFKSSHLDRLYRNGKLSWVQWYAGDWYRNQHARCSFALSVVASYGGGTGGGE
                     PSYGLPRTEAQLRARQLYMEARCALPAPMLGFMERMLIHDELPRAYGGKQRMRSTARI
                     AAALDALAGWLGLAAGQRLVRGGIDAGVDRAGTAEAAAEAVVRPLEQRVAAIEAALDP
                     VRPVDPAFLDDQG"
BASE COUNT          315 a          654 c          756 g          242 t
ORIGIN      
        1 cttcctgtgg ccctccccgg ccgcaagtac cacttgcgga cgatcgtcgt gagcgccgac
       61 ctccttgccc ggctgatcgc caccgggacg ccgcccgatc tggtggccga ggtggcgcgg
      121 ctggaggctc gcgccgcgat ggtggaggaa gcgctcgaag gccggcgcgc cgccgaccgc
      181 aaccggcagc agaaccgccg cggcgcccgt gcgtcacgtg acataacgtt aggtagcgtg
      241 acgacacgtg atgtcacgga cgccccccct cctgataaag aaaagtctcc cagaccctct
      301 aaagaaatta acctcccccg ccccatcgat gaagctgacg cttcatcgac aggccccaaa
      361 aatccggttt cgccggatcg gccgaaaaag ggtgggagcg agcgcccgga tcgccaacag
      421 cctgaccgcc agcccggcga ccgccaaccg tccgaacggc agcgcaaggg cgcccgcatc
      481 cccgacgact ggacgccgcc gctgatcgac gatctgccgc cgatggcgca ggcgctggcc
      541 tcgcaatggc cggccggagc ctatgccgcg cagggcgagc tgttcgcggc ctactggcgc
      601 ggcgaaggcg ggcggcaggg cgcccggaag ctcgactgga cgcagacctg gtgcaactgg
      661 atcggccggg tgaccgggca ggtgctgcgc gacgccaagg ccggggtgag gttcgccgcc
      721 gacgcgccgg cgcggcagag gaccgcggcg gagatcgagc gcgacgagcg gctggcgcgc
      781 gaactcgacg cgctgcaccg ggccgagacg gcggaagcga gggcgatccg ggagcggatc
      841 cgtcgcgatg ccggcgcccg gacctatgac ggctgggtga agccttgcgc catcgccatc
      901 gagggcgacg acatcatcgt cagcagcccc tccgaattcg tctccggctg gctcgagcag
      961 catttcgcgg cgaccttcac cggcgtcggt ggaacggtgc tcggccggcc ggtgcggcaa
     1021 gtgctgttca gggtggtgaa gccgcccgga tagcccctgc aacggctgtg gaggatcgat
     1081 tctgttggga gtgagcgtgg tggcggacga tgcggagaat gtgaggggct gggccaggcg
     1141 gcggctggcg gcgctggaag gcgcggagcg gccggagcgg gaagcgcgcg ggccgagctt
     1201 cttcaagcgc ctggcgctgc gcgaggcgga gcgggtcgag aacatactcg cggccgacaa
     1261 gatcggcgcc gagcgcaaga ccgggccgag ccgccgccag cgcgcccgca tggtcgtccc
     1321 caccgacgag cggatcggcc aggcgagcgc cggcggcgag cagctcgaat gggtgaaccc
     1381 cgccgagatc gacagcgccg agcaggcgat cggactgacc cgccgcttca aatcctccca
     1441 tctcgaccgg ctctaccgca acggcaagct gagctgggtg caatggtatg ccggcgactg
     1501 gtaccgcaac cagcatgcgc ggtgcagctt cgcgctgtcg gtggtggcga gctatggcgg
     1561 cgggaccggc ggcggcgagc cgagctacgg cctgccgcgc accgaggcgc aattgcgggc
     1621 gcggcagctc tacatggagg cgcgctgcgc gctgccggcg ccgatgctcg gcttcatgga
     1681 gcggatgctg atccacgacg agctgccgcg cgcctatggc ggcaagcaga ggatgcgcag
     1741 cacggcgcgg atcgcggcgg cgctggacgc gctggcgggg tggcttgggc tggcggccgg
     1801 gcagcggctg gtgcgagggg gtatcgacgc cggcgtcgac agggcgggaa cggccgaggc
     1861 ggcggccgaa gcggtggtgc gcccgctcga gcagcgggtg gcggcgatcg aggcggcgct
     1921 ggacccggtg cggccggtcg accccgcctt cctggacgat cagggca
//
LOCUS       DASVRK010000135        12231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_273761, whole genome shotgun sequence.
ACCESSION   DASVRK010000135 DASVRK010000000
VERSION     DASVRK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273761"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..2593)
                     /locus_tag="VF589_05185"
     CDS             complement(170..2593)
                     /locus_tag="VF589_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX8257005.1"
                     /translation="MPRRSTDGTLPSRQQILDFIEGSDGPAGKREIAKAFGLSGNDKI
                     ALKGLLRDMADEGLIDSAPGRAFHKMGGVPKVTVLRVVDVDDSGNAWAVPERWEAEGP
                     EPRLRLMEKGRSAVGVGDRILARTEAKGNGYIAHPMKKLLKGSELVLGVVHQEGENFW
                     LRPVEKKERRELPISDRGKAEVGDLVLAEKTGRAPRITARVSDVLGDPFAPRSFSLIA
                     IHKHGIPHAFPDAVLAEAERVAGQDLGPREDLTHVPFVAIDPADARDHDDAVWAAPDD
                     DAANAGGWKAIVAIADVSFYVRPGSALDKEARKRGNSVYFPDRVVPMLPETLSAHVCS
                     LVEGEDRAALVCHLQVSKDGQLKSWRFTRARVRIAANIAYEDAQAAIDAAEGVEPAPS
                     VRPEPVEGHSLPSSGSSQEGEGFDRLSPNGSEPVEVSSSPCSMPELEPSLPPVAPEIL
                     ETTLKPLWACWNALYRAREKRGPLELDLPERRVMLDEKGRILSVAPRERLDAHKLIED
                     YMIAANVAAAKALEARKAPVMYRDHEPPSREKLVALKDYLKTFEIEFALGQVIRPDTF
                     NRILERVGEADFKPQIMEQVLRSQTQAYYGPENHGHFGLALGSYAHFTSPIRRYADLI
                     VHRSLVRAYNLGEGGLSDSEAEAMEVTGELISQLERRAMEAERETIDRYVAAYLSERV
                     GDLVDCRITGVQPFGFFATVEGLGGDGLVPVSTLGSDYFRYDEASQTLTGDDSGDVFA
                     SGQRLKLRLVEANPVSGGLRFELPGAAGGGGDRRPRRDGRRGAAPDGKRRLQGKRGRP
                     GNIRHKGRK"
     gene            complement(2728..4167)
                     /locus_tag="VF589_05190"
     CDS             complement(2728..4167)
                     /locus_tag="VF589_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8257006.1"
                     /translation="MKNRSLLLALVPLLIAAAPAARPAATPAAVAAEVREERLRAIVE
                     RLVSFGTRHTLSSQTDRKRGIGASLRWTEAEFRRYARACRGCLTVATPSETVTGRRIP
                     NPTLVRNVFAIQRGTVHPNRVVIISGHIDSRVSNVMDAAKDAPGANDDGSGTAAVIEA
                     ARVLSRHAFPNTIVYAALSGEEQGLYGGKIMADYARAQGWEVIANLNNDIIGNSCGSD
                     GHCDPAHVRVFSEGPRWQGREDLAARQRSLGGENDSPSRNLSRWLDSFADDAKLGLDV
                     RQIWRNDRFGRGGDHTEMLNAGYPAIRFSVAVENYNHQHQDIRVEKGVEYGDTIDKMD
                     FDFLEKVVRLNVAALAALASAPMPPEPRVEGAVSTDTIVRWQTVPGALRYIIRWRRTD
                     ANQWQHSLRSGEENRFDCGTSGSTTVYAANGARVSRVCRWTLPSIRVDDWVFGVSSIS
                     RDGYESPVASAVPGGAFRPYTPPPAPAGQ"
     gene            4291..4515
                     /locus_tag="VF589_05195"
     CDS             4291..4515
                     /locus_tag="VF589_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257007.1"
                     /translation="MTRVLDRAEADTAEHKELGPDLAVTAIAIVSALLNAAIFRLIRI
                     RRAAGRRSIRASAAQAAVTVCSMVPKMAWP"
     gene            complement(4464..5471)
                     /locus_tag="VF589_05200"
     CDS             complement(4464..5471)
                     /locus_tag="VF589_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8257008.1"
                     /translation="MKLGSLKGGRDGRLVVVSRDLAWCAEATHIAPTLQAALDDWARV
                     GPQLELLATDLAHEAIPMMRFHERAAAAPLPRAYQWADGSAYVNHVQLVRQARGAEMP
                     ESFWTDPLMYQGASDEMLAPRDPIPLADEAWGCDLEAEVVVVTGDVPRGVTREQALEH
                     ILLVGLVNDVSLRNLIPAELAKGFGFLQSKPASALSPVFVTPDELGPRWREGKLHGAL
                     KVDLNGAPFGRADAGEDMTFDFGTLVAHLARTRNLGAGTIVGSGTVSNRDAGGGPGKP
                     IGEGGLGYSCIAEVRTVETILAGKPATPFLKAGDSVRIWMEDDQGHAIFGTIEQTVTA
                     A"
     gene            5584..7095
                     /locus_tag="VF589_05205"
     CDS             5584..7095
                     /locus_tag="VF589_05205"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX8257009.1"
                     /translation="MRNIDHFIGGGAYASRERQDSGRSGDIFDPNNGGIQARVRLGTA
                     DDLAKAVDNARNAFPTWAATNPQRRARVMFNFKTLVEANMDALAHLLSSEHGKVIADA
                     KGDVQRGLEVIEFACGIPHALKGEYTQGAGPGIDVYSMRQPLGIVAGITPFNFPAMIP
                     MWMFGVAIAVGNAFILKPSERDPSVPVRLAELMKEAGLPDGILQVVHGDKEMVDAILD
                     HEDVKAISFVGSSDIAHYIYSRGTANGKRVQAFGGAKNHGIVMPDADLDMVVNDLAGA
                     AFGSAGERCMALPVVVPVGDKTADNLREKLVPAIEALRVGVSTDAEAHYGPVVNAAHK
                     QRIENYIQMCADEGGELVVDGRGFSLQGHEEGFFIGPTFFDHVKPNFRSYQEEIFGPV
                     LQMVRADSFEEAVRLPSEHQYGNGVAIFTRNGHAAREFAARVNVGMVGINVPIPVPVA
                     YHTFGGWKRSGFGDINQHGMEGVRFWTKTKTITQRWPDGSVTGENAFVIPTMG"
     gene            7106..7714
                     /gene="gstA"
                     /locus_tag="VF589_05210"
     CDS             7106..7714
                     /gene="gstA"
                     /locus_tag="VF589_05210"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX8257010.1"
                     /translation="MKLYYAPGACSQAPHILLREAGLDFDPVQVDLRAKRLADGGDYL
                     AINPKGAVPALGFDDGAVLTENAAILQYIGDQAPDTGLLPRVGEFERYRALEWLNYIA
                     TELHKGFAPLWNPASTPEAKDATRDLLAKKFDYVQARLGDNPFLLGEHFTAPDAYLFV
                     VLNWTRVHAIPLSRWPGLADYARRIAHRPAVQAALRAEGLVN"
     gene            7719..8861
                     /locus_tag="VF589_05215"
     CDS             7719..8861
                     /locus_tag="VF589_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8257011.1"
                     /translation="MTQFDLNDEQRQIQDLARQFTADAITPHAAEWDEKHIFPRDTIR
                     AAAELGFGSIYVSEESGGIGLGRLEAALIMEAMAYGDPSTSAFISIHNMASWMIDRFG
                     SPTVKQKYLPSMVTMERMGSYCLTEPGSGSDAAALKTRAVKDGDSYVVSGSKAFISGA
                     GENEIYVTMARTGAEGPKGISALVIEKDMPGVSFGAQERKLGWHSQPTAQVNFDEVRV
                     PAENLVGSEGQGFRIAMMGLDGGRLNIGACSLGGAQRCLDESVSYTKERKQFGQPIAD
                     FQATQFTLADMETELQAARMLLYAAAVKVTENAPDKSKFAAMAKRLATDTGSSVVDRA
                     LQLHGGYGYLMDYPIERFWRDLRVHSILEGTNQIMRVIIGRDMLRQ"
     gene            8858..9907
                     /locus_tag="VF589_05220"
     CDS             8858..9907
                     /locus_tag="VF589_05220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8257012.1"
                     /translation="MTSDVLTFVEGRIGRIRLNRPKAIHALTREMCVAMTAALLEWRE
                     ARAAKAVLIDHAEGRGFCAGGDVRMLAESGAADGAEARAFFFDEYRLNHQLFTYQLPT
                     IAFMDGITMGGGVGISQPCRYRVATENTRFAMPETTIGLFPDVGGGWYLPRLPGRIGQ
                     YLALTGARLDGAECRALGLATHYVPSAALDAVKARIGEQPEGVEAILDQAAVPTPEPR
                     IVADAERIDRLFVADRFEDLLAALEEDGSDWAGKTLALLRTKSPQACKVSLRLLADGA
                     RMHDFADEMRQEYAVASRVVQRHDFVEGVRSLLVDKDNQPRWQPETPDAVTDHAIDTI
                     FAPMPDGEEWTPLGV"
     gene            9956..10729
                     /locus_tag="VF589_05225"
     CDS             9956..10729
                     /locus_tag="VF589_05225"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8257013.1"
                     /translation="MTYENILVEQRGAVTLITLNRPKALNALNSGVLRELIHAFAAYD
                     ADDGQRCAVLTGNERAFAAGADIKEMADQGFASMYGSNFFQGWEKVTATRKPWIAAVA
                     GYALGGGCEVAMMADFIIAADTAQFGQPEIKLGVTPGMGGSQRLTWAVGKAKAMEMCL
                     TGRNMGAEEAERAGLVAKVVPAADLLDEALKTAGTIAGMAPLAAMATKEQVNAAFEMS
                     LAQGIVFERRLFHGLFGTEDQKEGMAAFVAKRKGEWTGR"
     gene            10883..11824
                     /gene="mmsB"
                     /locus_tag="VF589_05230"
     CDS             10883..11824
                     /gene="mmsB"
                     /locus_tag="VF589_05230"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX8257014.1"
                     /translation="MARIAFIGLGNMGGGMAANLAKAGHEVRAYDLSPDALDKAKRSG
                     CLPLGSATEAVRGVEAVVTMLPAGKHVREVYESSVLGNAPPNAILIDCSTIDVTTARE
                     EIARAEREGYRMVDAPVSGGIAAADAGTLTFMVGGTSEAFEQAQPLLELMGKAVIHAG
                     GPGSGQAAKICNNMLLGASMIATCETFVLAEKLGLDPQTFFEISSQASGQCWSMTSYC
                     PVPGVGPQTPADRNYDGGFAAALMLKDLRLAMEAAQSVDAYTPMGSHAEELYTRFAEK
                     LGGSGKDFSGIIKMIDDSWQSPEGAPPSPPPGVRFPR"
     gene            complement(11835..>12231)
                     /locus_tag="VF589_05235"
     CDS             complement(11835..>12231)
                     /locus_tag="VF589_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX8257015.1"
                     /translation="AAVITDSGGVQEETTYLGIPCLTLRENTERPITIEQGTNRLVRA
                     ETLALALSRALAAPRDTLRRPFLWDGATAARCVADLRRRSGPFRHAAEELTATEPASI
                     LAPAAPEARFRGNDDRRQAGGHRRYRRAS"
BASE COUNT         1996 a         4067 c         4224 g         1944 t
ORIGIN      
        1 ctcgaaaatt gcgaggtgac gcagagcagc ggatattccc ggctcgaccg cgagacttgc
       61 cggatgctga tccaacatgc ccggttcacc ccgaagatga ccgagaatgg ccgccgcatg
      121 cgctcgaccc gcgacggcaa gatcgtctgg aagatcagct aggtccgctt cacttccgac
      181 ccttgtgccg gatattgccg gggcggccgc gcttgccctg aaggcgccgc ttgccgtccg
      241 gcgcggcgcc gcgccggccg tcgcggcgcg ggcggcgatc gcccccgccg ccggcggcgc
      301 cgggtaattc gaagcgcagg ccgcccgaga ccgggttggc ctcgaccagc cgcaatttca
      361 gcctctggcc cgaggcgaag acgtcgccgc tgtcgtcgcc ggtgagggtc tggcttgcct
      421 cgtcgtaacg gaaataatcg ctgccgagcg tcgagaccgg gacgaggccg tcgccgccga
      481 ggccttcgac cgtggcgaag aagccgaacg gctgcacgcc ggtgatgcgg caatcgacga
      541 ggtcgccgac gcgctcggaa agataggcgg cgacatagcg gtcgatcgtc tcgcgctccg
      601 cctccatcgc gcggcgctcg agctgcgaga tgagctcgcc ggtgacttcc atcgcttccg
      661 cttcggaatc gctgagcccg ccctccccca gattgtaggc gcggaccagc gaacggtgca
      721 cgatgaggtc ggcgtagcgg cggatcgggc tggtgaaatg ggcgtaggag ccgagcgcca
      781 gcccaaaatg gccgtgattt tcggggccgt aataggcctg ggtctggctg cgcaggacct
      841 gctccatgat ctgcggcttg aaatcggcct cgccgacccg ctcgaggatg cggttgaaag
      901 tatccgggcg gatcacctgg ccgagcgcga actcgatctc gaacgtcttc agataatcct
      961 tgagcgcgac cagcttctcg cggctgggcg gctcgtggtc gcgatacatg accggcgcct
     1021 tcctcgcctc gagcgccttg gcggcggcga cattggcggc gatcatataa tcctcgatca
     1081 gcttgtgcgc gtcgagccgc tcgcgcggcg cgaccgagag gatgcggccc ttctcgtcga
     1141 gcatcacccg gcgctcgggc aggtcgagct cgagcggccc gcgcttctcg cgggcgcggt
     1201 agagcgcgtt ccagcaggcc cagagcggct tcagggtggt ttccaggatt tccggggcaa
     1261 cgggaggcag tgacggctcg agctccggca tcgagcaggg ggaggaggag acttcgacag
     1321 gctcagatcc gttcgggctg agcctgtcga agccctctcc ttcttggcta ctgccggagg
     1381 aaggcaagga atggccttcg acaggctcag gccgaacgga tggtgcgggc tccacccctt
     1441 ccgccgcatc gatcgcggcc tgggcgtcct catacgcgat gttggcggcg atgcggacgc
     1501 gggcgcgggt gaagcgccag ctcttcaact ggccgtcctt cgacacctgg aggtggcaga
     1561 ccaaggcggc gcggtcctcg ccctcgacca gcgagcagac gtgtgccgac aaagtctcgg
     1621 gcagcatcgg cacgacgcgg tcggggaagt aaacgctgtt gccgcgcttc cgcgcttcct
     1681 tgtcgagcgc cgagccgggg cggacgtaga agctcacgtc ggcgatggcg acgatcgcct
     1741 tccagccgcc ggcattggcg gcatcgtcgt cgggcgccgc ccagacggcg tcgtcatggt
     1801 cgcgcgcgtc ggccgggtcg atcgcgacga aaggcacgtg cgtgaggtcc tcgcgcgggc
     1861 cgaggtcctg gcccgccacc cgctccgcct cggccagcac ggcgtcggga aaggcgtgcg
     1921 ggatgccgtg cttgtggatg gcgatgaggc tgaagctgcg cggcgcgaac ggatcgccga
     1981 gcacgtcgga gacgcgggcg gtgatgcgcg gcgcccggcc ggtcttttcg gcgagcacga
     2041 ggtcgccgac ctcggccttg ccgcgatccg agatcggaag ctcgcggcgc tccttcttct
     2101 cgaccgggcg cagccagaaa ttctcgcctt cctggtggac cacgccgagg acgagttcgc
     2161 tgcctttcaa gagcttcttc atcggatggg cgatgtagcc gttgcccttg gcctcggtgc
     2221 gggcgaggat gcggtcgccg acgccgacgg cggagcggcc cttttccatc aggcgaaggc
     2281 gcggctccgg gccttcggcc tcccagcgtt cgggcaccgc ccaggcgttg ccgctgtcgt
     2341 cgacgtcgac cacgcgcagc acggtgacct tgggcacgcc gcccatcttg tggaaggcgc
     2401 ggccgggggc gctgtcgatc aggccctcgt cggccatgtc gcggagcagg cccttcaggg
     2461 cgatcttgtc gttcccggag aggccgaaag ccttcgcgat ctcgcgcttg ccggcggggc
     2521 cgtcggagcc ttcaatgaag tcgagaatct gctggcggct gggaagggtc ccatcagtgg
     2581 atcttcgtgg caattcgatc tcccgtcccg ctcatgctga ggagcggctg agcctgacga
     2641 agtcgcgtct cgaagcgcgg ccggtccttc gagacggcat ttcgccaagc tcaatgcctc
     2701 ctcaggatga gcggttcccg atcaatgcta ctgacccgcc ggagcaggcg gcggcgtata
     2761 gggccggaaa gcgccgcccg gcacggccga ggcgaccggg ctttcgtagc cgtccctgga
     2821 gatcgaagag acgccgaata cccaatcgtc gacgcggatg ctggggaggg tccagcgaca
     2881 aacacgtgac acgcgcgctc cgtttgccgc gtagacggta gtggacccgc tagtgccaca
     2941 gtcgaaccgg ttctcctccc ctgatcgcaa cgaatgctgc cactgattgg catcagtccg
     3001 gcgccaacga atgatgtacc tcagagcacc ggggacggtt tgccacctaa caatcgtatc
     3061 ggtgctcacc gccccctcga cgcgcggctc cggaggcatc ggcgcgctcg cgagggcggc
     3121 cagcgcggcg acgttgagcc gcaccacctt ctccagaaag tcgaaatcca tcttgtcgat
     3181 ggtgtcgcca tattcgacgc ccttctcgac cctgatatcc tggtgctggt ggttgtaatt
     3241 ctcgaccgcg accgagaagc ggatggccgg gtagccggcg ttgagcatct cggtatggtc
     3301 gccgccgcgg ccgaagcggt cgttgcgcca gatctggcga acgtcgaggc cgagcttcgc
     3361 atcgtcggcg aagctgtcga gccagcgcga caggttgcgc gacgggctgt cattctcgcc
     3421 gccgaggctg cgctggcggg cggcgagatc ctcccgcccc tgccagcgcg ggccttcgga
     3481 gaagacgcga acgtgggccg gatcgcaatg cccgtccgag ccgcagctat tgccgataat
     3541 gtcgttgttg aggttggcga tcacctccca gccctgcgcc ctggcgtaat cggccatgat
     3601 cttgccgccg tagaggccct gctcctcgcc cgacagcgcg gcatagacga tggtgttggg
     3661 gaatgcgtgg cgcgagagca cgcgcgccgc ctcgatcacg gcggcggtgc cggagccgtc
     3721 gtcattggcg ccgggcgcgt ccttagccgc gtccatcacg ttcgagacgc ggctgtcgat
     3781 atggccggag atgatgacca cccggttggg atgaaccgtg ccgcgctgga tggcgaagac
     3841 gttcctgacc agggtcggat tcgggatgcg gcggccggtt acggtttcag agggcgtcgc
     3901 gacggtgagg cagccgcggc aggcgcgggc gtagcggcgg aattccgcct cggtccagcg
     3961 caaagaggcg ccgatgccgc gcttgcggtc ggtctgcgag gacaatgtat gacgggtgcc
     4021 gaagctcacc agccgctcga cgatggcgcg cagccgctcc tcgcgaacct cggcggcgac
     4081 ggccgcggga gtcgcggccg ggcgagcggc gggagcggcg gcgatgagca gcggcacgag
     4141 cgcgagcagc aaagatctgt ttttcatggg cctagattgg ccaagccgcc cgcccttggc
     4201 aacagcggcc tgatcccctc ccctgtcacc tcacggacag gtcggcgcgg gtgcggaacg
     4261 agttcaaaag gcgcggcgtt ggcggcccct atgacgaggg tattggatcg cgccgaggcg
     4321 gacacggcag agcataaaga gctcgggccc gacctagcgg tgaccgccat cgcgatcgtc
     4381 tcggcgctgc tcaatgccgc catcttccgc ctgatccgga tccgccgcgc cgctggccgg
     4441 cggtccataa gagccagcgc cgctcaggcg gcggtaacgg tctgctcgat ggtgccgaag
     4501 atggcgtggc cctgatcgtc ctccatccag atccggacgc tgtccccggc cttcaggaac
     4561 ggcgtcgccg gcttgccggc gaggatggtc tccacggtgc ggacttcggc gatgcagcta
     4621 tagccgagcc cgccctcgcc gatcggcttg ccggggccgc cgcccgcgtc acgattggag
     4681 acggtgcccg agccgacgat ggtgccggcg ccgagattgc gggtcctggc gaggtgcgcg
     4741 accagcgtcc cgaaatcgaa ggtcatgtcc tcgccggcgt cggcgcggcc gaacggggca
     4801 ccgttcaggt ccaccttgag cgccccgtga agcttgccct ccctccagcg cggcccgagc
     4861 tcgtccgggg tcacgaacac cggcgacagc gccgaggcgg gcttggactg gaggaagccg
     4921 aagcctttgg cgagctcggc cgggatgagg ttgcgtaagg acacgtcgtt gaccaggccg
     4981 accagcaata tgtgctccag cgcctgctcg cgcgtcacgc cgcgggggac gtcgccggtg
     5041 accacgacca cttccgcctc gaggtcgcag ccccaggctt cgtcggcgag cgggatcgga
     5101 tcgcgcgggg ccagcatctc gtcggacgcg ccctggtaca tcagcgggtc ggtccagaag
     5161 ctttccggca tctccgcccc ccgcgcctgc cggacgagtt ggacatggtt cacataggcc
     5221 gagccgtccg cccattgata ggcgcgcggc aacggcgccg cggcggcgcg ctcgtggaag
     5281 cgcatcatcg ggatcgcttc gtgcgcgagg tcggtggcga gcagctcgag ctgcggcccc
     5341 accctcgccc aatcgtcgag cgcggcctgc agcgtcggcg cgatgtgagt cgcttccgcg
     5401 caccaggcga ggtcccggct caccaccacc agccggccgt cgcggccccc tttaagcgaa
     5461 ccaagcttca ttcttcctcc tactcctccc cgggacggga ggaatttggg gctctctggc
     5521 attccgccgg aagcttggct agtgccctcc ccaatccaag tctcacgagt ccaaggacat
     5581 cccatgcgga acatcgatca tttcatcggc ggcggcgcct atgcgtctcg cgagcggcag
     5641 gacagcggcc gctcgggcga catattcgac ccgaataatg gcggcatcca ggcccgggtc
     5701 cggctcggca ccgctgacga tctcgccaag gcggtcgaca atgcccgcaa cgcctttccc
     5761 acctgggccg ccaccaaccc gcagcgccgc gcccgggtga tgttcaactt caagacgctg
     5821 gtcgaagcga acatggacgc gctcgcccat ttgctctcgt ccgagcatgg caaggtgatc
     5881 gccgacgcca agggcgacgt gcagcgcggg ctcgaggtaa tcgaattcgc ctgcggcatc
     5941 ccacacgcgc tgaagggcga atatacgcag ggcgccgggc cggggatcga cgtctattcg
     6001 atgcgccagc cgctcggcat cgtcgccggc atcaccccgt tcaactttcc ggcgatgatc
     6061 ccgatgtgga tgttcggcgt ggcgatcgcg gtcggcaacg ccttcatcct gaagccctca
     6121 gagcgcgacc cgtcggtgcc ggtgcggctc gccgaactga tgaaggaggc ggggctgccc
     6181 gacggcatcc tccaggtggt gcacggcgac aaggagatgg tcgacgccat cctcgaccat
     6241 gaggacgtca aggcgatcag cttcgtcgga tcgtccgaca tcgcccatta tatctacagc
     6301 cgcggcactg ccaacggcaa gcgcgtgcag gcgttcggcg gcgccaagaa ccatggcatc
     6361 gtcatgcccg acgccgacct cgacatggtg gtgaacgacc tggccggcgc cgccttcgga
     6421 tcggccggcg agcgctgcat ggcgctcccc gtcgtggtgc cggtcggcga caagaccgcg
     6481 gacaatctcc gcgagaagct ggtgccggca atcgaggcgc tgcgggtggg agtctcgact
     6541 gatgcggagg cgcattacgg cccggtggtc aacgccgccc acaagcagcg catcgaaaat
     6601 tacatccaga tgtgcgccga cgagggcggc gagctggtgg tggacggccg cggcttcagc
     6661 ctgcaggggc acgaggaggg cttcttcatt ggccccacct tcttcgacca tgtgaagccc
     6721 aatttccgct cctatcagga ggagattttc ggccccgtcc tgcagatggt ccgcgccgac
     6781 agcttcgagg aggcggtccg gctcccttcc gagcaccagt acggcaacgg cgtcgccatc
     6841 ttcacccgca acggccatgc ggcgcgcgag ttcgcggccc gggtcaacgt cggcatggtc
     6901 ggcatcaacg tgccgatccc ggtgccggtc gcctaccaca cgttcggcgg ctggaagcgc
     6961 tcgggcttcg gcgacatcaa ccagcatggc atggagggcg tccgcttctg gaccaagacc
     7021 aagaccatca cccagcgctg gccggacggc tccgtcaccg gcgagaatgc gttcgtcatc
     7081 ccgacgatgg gctaaaggag ggaccatgaa gctctattac gcccccggcg cctgctcgca
     7141 ggccccgcac atcctgctcc gcgaggccgg gctcgacttc gatccggtcc aggtcgacct
     7201 gcgcgccaaa cggctcgccg acggcggcga ttatctggcc atcaacccga agggagcggt
     7261 gcccgcgctc ggcttcgacg acggcgccgt gctcaccgag aatgcggcga tcctgcaata
     7321 tatcggcgac caggcgcccg atactggcct cctcccccgc gtcggcgagt tcgagcgcta
     7381 tcgggcgctc gaatggctga actatatcgc gaccgagctt cacaagggct tcgcgccgct
     7441 ctggaacccg gcttccacgc ctgaagccaa ggacgcgacc cgggacctgc tcgccaagaa
     7501 gttcgactat gtccaggcgc ggctgggcga caacccgttc ctgctcggcg aacatttcac
     7561 cgcccccgac gcctatctgt tcgtggtcct caactggacg cgggtccacg ccatcccgct
     7621 gtcgcgctgg cccggccttg ccgactatgc gcgccgcatc gcccatcgcc ccgccgtcca
     7681 ggcggcgctg cgcgcggaag ggctggtgaa ctgagccgat gacccagttc gacctcaatg
     7741 acgaacagcg ccagatccag gatttggcac gccagttcac cgccgacgcc atcacgccgc
     7801 atgcggccga atgggacgag aagcacatct tcccgcgcga cacgatccgc gccgcggccg
     7861 agctcggctt cggatcgatc tacgtgtccg aggagagcgg cgggatcggc ctggggcggc
     7921 tcgaggcggc cctgatcatg gaggcgatgg cttatggcga tccctccacc tcggccttca
     7981 tctcgatcca caatatggcc agctggatga tcgaccgctt cggatcgccc accgtgaagc
     8041 agaaatatct gccctcgatg gtgacgatgg agcggatggg ctcctattgc ctgaccgagc
     8101 cgggctcggg ctccgacgcg gcggcgctga agacccgggc ggtgaaggac ggcgattcct
     8161 acgtcgtctc cggctccaag gcgttcatct ccggcgccgg cgagaacgaa atttacgtga
     8221 ccatggcccg aaccggtgcc gaggggccga aggggatcag cgcgctggtc atcgaaaagg
     8281 acatgccggg cgtcagcttc ggcgcgcagg agcgcaagct cggctggcat tcgcagccga
     8341 ccgcgcaggt caatttcgac gaggtgcggg tgccggcgga aaatctggtc gggtccgagg
     8401 gccagggctt tcggatcgcg atgatggggc tcgacggcgg gcggctcaac atcggcgcct
     8461 gctcgctcgg cggcgcccag cgttgcctcg acgaatcggt gagctacacc aaggaacgca
     8521 agcagttcgg ccagccgatc gccgacttcc aggcgaccca gttcacgctc gccgacatgg
     8581 agaccgagct gcaggcggcg cggatgctgc tttacgcggc ggcggtgaag gttaccgaga
     8641 acgcgcccga caagtccaaa ttcgcggcga tggcgaagcg actcgcgacc gacaccggct
     8701 cgtcggtggt cgaccgcgcg ctccagctcc acggcggcta cggctatctg atggactatc
     8761 cgatcgagcg cttctggcgc gaccttcgcg tccattcgat cctcgagggc accaaccaga
     8821 tcatgcgggt gatcatcggc cgggacatgc tgcggcagtg acctccgacg tgctgacgtt
     8881 cgtcgagggc aggatcggcc gcattcgctt gaaccgcccg aaggcgatcc atgccctgac
     8941 gcgcgagatg tgcgtggcga tgacggcggc tttgctggaa tggcgcgagg cgcgggcggc
     9001 gaaggcggtg ctgatcgatc atgccgaagg ccgcggcttc tgcgccggcg gcgacgtccg
     9061 gatgctggcc gagagcggcg cggccgacgg ggcggaggcg cgcgccttct ttttcgacga
     9121 atatcggctg aaccaccagc tcttcaccta ccagctccca accatcgcct tcatggacgg
     9181 catcaccatg ggcggcggcg tcggcatctc gcagccctgc cgctaccggg tcgcgaccga
     9241 gaatacccgg tttgccatgc ccgagacgac catcggcctg ttccccgacg tcggcggcgg
     9301 ctggtacctg ccgcggctgc cgggccgcat cggccagtat ctcgcactca ccggcgcgcg
     9361 gctcgacgga gcggaatgcc gagcgctcgg ccttgccacc cattatgtgc cgtcggcggc
     9421 gctggacgcg gtcaaggccc ggatcggcga gcagccggag ggggtcgagg cgatactcga
     9481 tcaggccgcg gtgccgacgc cggagccccg cattgttgcg gacgccgagc ggatcgaccg
     9541 gctgttcgtg gccgaccggt tcgaggattt gctggcggcg ctcgaggagg acggctccga
     9601 ctgggcgggg aagacgctcg ccctgctccg caccaagagc ccccaggcct gcaaggtgtc
     9661 gctgcggctg ctcgccgacg gcgcgcggat gcacgatttc gccgacgaga tgcggcagga
     9721 atatgcggtc gcttcgcggg tcgtgcagcg ccacgatttc gtcgagggcg tgcgctcgct
     9781 gctggtcgac aaggacaacc agccgcgctg gcagccggaa acgccggacg cggtgaccga
     9841 ccatgcgatc gacaccatct tcgcgcccat gcccgacggc gaggagtgga cgccgttggg
     9901 ggtctagtaa caggaaacga ggttccggcc tccgccggga cgacgagagg tggctatgac
     9961 gtacgagaac atcctggtcg aacaacgcgg cgcggtgact ttgatcacgc tgaaccggcc
    10021 gaaggcgctg aatgcgctca acagcggcgt gctgcgcgag ctgatccatg ccttcgccgc
    10081 ttatgatgcc gacgatggcc agcgctgcgc ggtgctgacc gggaacgagc gcgccttcgc
    10141 ggccggcgcc gacatcaagg agatggccga ccagggcttc gccagcatgt acggctccaa
    10201 cttcttccag ggctgggaga aggtgacggc gacgcgcaag ccctggatcg cggcggtcgc
    10261 cggctatgcg ctcggcggcg gctgcgaagt ggcgatgatg gcggacttca tcatcgccgc
    10321 cgacaccgcc cagttcggcc agcccgagat caagctcggc gtgacgccgg gcatgggcgg
    10381 gtcgcagcgc ctgacctggg cggtcggcaa ggccaaggcg atggaaatgt gcctcaccgg
    10441 ccgcaacatg ggcgccgagg aagcggagcg ggcgggcctt gtcgcgaaag tagtgccggc
    10501 cgcggacctc ctggacgagg cgctgaagac ggccgggacc atcgccggca tggccccgct
    10561 cgccgcgatg gcgaccaagg agcaggtcaa tgctgccttc gaaatgagcc tggcgcaggg
    10621 tatcgtgttc gagcggcggc tgttccacgg cctgttcggc accgaggacc agaaggaagg
    10681 catggccgcc ttcgtcgcga agcgtaaggg ggagtggacg ggacggtaag gtgtcgtcat
    10741 cccagcgaaa ctgatcgtca tcccagcgaa agctgggatc tcccttcccc ttcccccttg
    10801 aagaaaagtg agattccagc tttcgctgga atgacgggag cttcgccgga ttgacgcccc
    10861 tccacgcgct gaaggaatcg caatggcacg tatcgcattt atcgggctcg gcaatatggg
    10921 cggcgggatg gccgcgaacc ttgccaaagc ggggcacgag gtccgggcct acgacctctc
    10981 gcccgacgcg ctcgacaagg cgaagcggag cggctgcctg ccgctgggat ccgccaccga
    11041 ggcggtgcgc ggcgtcgagg cggtcgtcac catgctgccg gccggcaagc atgtccgcga
    11101 ggtctatgaa agcagcgtgc tcggcaatgc gccccccaac gcgatcctga tcgattgctc
    11161 gaccatcgac gtcacgaccg cgcgcgagga gatcgcccgc gccgaacgcg agggctatcg
    11221 gatggtcgac gcgcccgtgt cgggcgggat cgccgcggcc gatgcgggca cgctcacctt
    11281 catggtcggc ggcacgagcg aagcattcga gcaggcgcag cccttgctgg agctgatggg
    11341 caaggcggtg atccacgctg gcggccccgg ctcgggtcag gccgccaaga tctgcaacaa
    11401 catgctgctc ggcgcctcga tgatcgcgac ctgcgagacc ttcgtgctgg ccgaaaagct
    11461 tggcctcgac ccgcagacct tcttcgagat ttccagccag gcctctgggc aatgctggtc
    11521 gatgaccagc tattgcccgg tccccggagt gggcccgcaa acccccgcag accgcaatta
    11581 tgacggcggg ttcgcggcgg ccctgatgct caaggattta cggctggcga tggaggcggc
    11641 gcagagcgtc gacgcctata cgccgatggg atcgcacgcc gaggagctct acacccgctt
    11701 tgccgagaag ctcggcggat cgggcaagga tttttccggg atcatcaaga tgatcgacga
    11761 cagctggcag tcgcccgaag gcgctccgcc ctccccgccg cccggggtgc gctttccgag
    11821 atagagcgct ccggtcagga agcccggcgg tagcggcgat gaccgccggc ctgcctgcgg
    11881 tcgtcattgc cgcggaagcg ggcttcgggg gccgcggggg ctagaatcga ggcgggttca
    11941 gtcgcggtga gctcctccgc ggcgtggcgg aagggacctg agcggcggcg caggtccgcg
    12001 acgcagcggg cggcggtggc gccgtcccac aggaacggtc ggcgcagcgt gtcgcgcggc
    12061 gcagccagcg cccggctgag cgcaagcgcg agcgtctcgg cgcggacgag gcggttggtg
    12121 ccctgctcga tggtgatcgg ccgctccgta ttctcgcgca gcgtcaagca aggaataccg
    12181 agataggtcg tctcctcctg cacccccccg ctgtcggtga tcacggccgc g
//
LOCUS       DASVRK010000136        12750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_522641, whole genome shotgun sequence.
ACCESSION   DASVRK010000136 DASVRK010000000
VERSION     DASVRK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522641"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /gene="sufB"
                     /locus_tag="VF589_05240"
     CDS             <1..801
                     /gene="sufB"
                     /locus_tag="VF589_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX8257016.1"
                     /translation="NTGQFERTLIVADKGSYVSYLEGCTAPMRDENQLHAAVVELIAL
                     DDAEIKYSTVQNWYPGDAEGRGGIYNFVTKRAMCAGDRSKVSWTQVETGSAITWKYPS
                     CILKGEGSVGEFYSVALTNNMQQADTGTKMVHIGKNSRSTIVSKGISAGRSNNTYRGL
                     VRVNAGAENVRNFTQCDSLLLGDQCGAHTVPYIEVKNPTATIEHEATTSKISDDQMFY
                     ARQRGLNAEDAVALIVNGFAKEVLQQLPMEFAVEAQKLLGISLEGSVG"
     gene            1066..1809
                     /gene="sufC"
                     /locus_tag="VF589_05245"
     CDS             1066..1809
                     /gene="sufC"
                     /locus_tag="VF589_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX8257017.1"
                     /translation="MLRIENLHAEIDGKQILNGLTLSVGAGEVHAIMGPNGAGKSTLG
                     YVLGGRPGYEVTEGSVTLDGRDLLDMEPDERAAAGLFLGFQYPVEIPGVSNVQFLRTA
                     LNAQRRARGEAEASAGDFLRLAKEQGAALDVSMDMLKRNVNVGFSGGEKKRNEMVQMG
                     IIAPRLAVLDETDSGLDIDALRIVGEGINRIMRAPDKAVLLITHYQRLLDLVEPDFVH
                     VLAEGLIVRSGGPELAHLLEREGYGAIAA"
     gene            1955..3070
                     /locus_tag="VF589_05250"
     CDS             1955..3070
                     /locus_tag="VF589_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX8257018.1"
                     /translation="MNVLHLPSNRTEAWRWSDLSALPEIAARQPAGTVPEALPWIACA
                     NEGPRLLFVDGRLAAERSNLGTIEIGAIEPEPRDHALARQVGREGWKLKLGRDHAPQG
                     LVQIVHVSTGAADHLPAEVELDVDAQASIVETFIGHGWTNRLSHFRLFKGARLMLARR
                     LMSDAGPSASLRTSFISLTDRAVVGEGASFILTTLAAGGADTRLDGEVRIAGEAGFAE
                     VGGALLARGRGRHDANLVVRHEAVGGVSRQVWRSVADDRATCSVAARVEVARAAQKTD
                     GEQSLKGLLLDRGATINAKPELEIFADDVKCAHGATVGELNRDALFYLASRGVPPQEA
                     KALLTQAFVADAIDRTGEPAVRDAFHADALAWLGGTR"
     gene            3235..4416
                     /locus_tag="VF589_05255"
     CDS             3235..4416
                     /locus_tag="VF589_05255"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX8257019.1"
                     /translation="MVSDFPAIPSGWAYLDTAATAQKPRPVIDAIARGYAETYATVHR
                     GVYQRSAEMTLAFEAARHGTAAFIGAPVPEEVIFVRGATEGINLVAQSWGGTFLKPGD
                     RIMLSMLEHHSNIVPWQLVAERTGAEIDVAPLTADGCIDLDAMAAMLTERHKMVALAH
                     VSNVLGSVLDARRAADLAHDVGALLLLDGCQAVPRMPVDVAALGCDFYVFSAHKLYGP
                     TGIGVLWGRGDLLDAMPPWQGGGAMIDKVSFEGTTFLPPPSRFEAGTPHVVGVLGLHA
                     AIDYVRAIGLDAIAAHEAALVRATRDALSAINSLRLFGPDDSAGIVSFAVEGVHPHDV
                     GTILDESRVAIRAGHHCAQPLMAHLGVDATARASFGVYNDRQDVEALVRGIERVTRIF
                     G"
     gene            4413..4928
                     /locus_tag="VF589_05260"
     CDS             4413..4928
                     /locus_tag="VF589_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEX8257020.1"
                     /translation="MNEERKIEIEEVSEAPKPPKARVAPPGATEGDRAEAGGGGSPLS
                     QAFERKKDYLAGFLAHKPEEAAPVEPGGNLYEAVIEALKEIYDPEIPVNIYDLGLIYD
                     VEITPEHHAMVKMTLTTPHCPVAESMPGEVELRVGSVPGIGHAEVELVWDPPWDPQKM
                     TDEAKLELGML"
     gene            5062..5415
                     /locus_tag="VF589_05265"
     CDS             5062..5415
                     /locus_tag="VF589_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX8257021.1"
                     /translation="MSTTTRARPAALNLTPAAEARIAALMARAPEGAIGVKLSTPRRG
                     CSGLAYSVDYVTEEKPFDEKIETPGGIFYVDGASILYLIGSRMDWKEDDFAAGFVFDN
                     PNAKGACGCGESFTV"
     gene            5547..6143
                     /locus_tag="VF589_05270"
     CDS             5547..6143
                     /locus_tag="VF589_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257022.1"
                     /translation="MLKPLLACMLLAAPAAISAAQTPAVQAPTAPQAAPGEWTVSGSA
                     QGCILYGSSAQGTVVSILAGPGQDALIFLVQNRQWSSLQDGSKHAVAVAFDGRNEWNF
                     EAVAKTELDSDGPGLLFAVKPGEKEGVNFITQFAAANGMRIDRDGQQLASLPVGDGNT
                     AMAALAQCMGKMYSGASEAAGEESTPVFQTSSEKPVSL"
     gene            6394..6663
                     /locus_tag="VF589_05275"
     CDS             6394..6663
                     /locus_tag="VF589_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEX8257023.1"
                     /translation="MTSNDFIRQLQGYGLTTAEISYYMPDHPKLIQLFVWQTYDVAPT
                     FPVLHGFLDHWRREIEAALHSVRVAHQHLIRPAEWRAVDGVISIH"
     gene            6800..7471
                     /locus_tag="VF589_05280"
     CDS             6800..7471
                     /locus_tag="VF589_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX8257024.1"
                     /translation="MQPALAHIDAWIFDLDNCLYPASTDLFALIDVRMGEYIARLLGV
                     DAAEARRVQKLYFHEHGTTLAGLMAAHGVDPHEFLDYVHDIDLARLAADPALVAALDR
                     LPGRKFVFTNGDEAYAGRVLARLGLADAFDGLHDIHAMDYLPKPNPASYAALCERHGI
                     APERALFAEDMARNLAPAKALGMTTVWVDNGSERGSHGADQSFIDYRVADIGDWLTEI
                     TGDEA"
     gene            7468..8295
                     /gene="dapD"
                     /locus_tag="VF589_05285"
     CDS             7468..8295
                     /gene="dapD"
                     /locus_tag="VF589_05285"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX8257025.1"
                     /translation="MTEELQHVIEDAWLRRESLDPSTSGPVRQAVVAAIAALDAGRVR
                     VAEKAEGGWQVNQWLKKAVLLSFRLNPMEAIAGGPGGAPWWDKVPSKFSGWDAAQFEQ
                     AGFRAVPGAIVRRGAFIGRGAVLMPSFVNIGAHVGEGTMVDTWATVGSCAQIGRNVHI
                     SGGAGIGGVLEPLQAGPVVIEDGAFIGARSEVAEGVIVEEGAVLSMGVFLGASTKIVD
                     RMTGQVFMGRVPAYSVVVPGSLPPKSGDGPALACAVIVKRVDAQTRSKTSINELLRD"
     gene            complement(8444..8980)
                     /locus_tag="VF589_05290"
     CDS             complement(8444..8980)
                     /locus_tag="VF589_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8257026.1"
                     /translation="MPLLLPLAACERQPAASDPAPVVAADLDLPRGKVDRSKAGTRAP
                     EASFEDPAGEPVTLAHFRGKPLLVNFWATWCAPCIVELPTLDALAAKGGNLEVLAVSQ
                     DIEGAKKVDAFFAARKFEALEPYLDTEMALMGELKVLTLPTTILYDAEGREVWRMTGI
                     EDWTGARAAGLLREAGVE"
     gene            9076..9315
                     /locus_tag="VF589_05295"
     CDS             9076..9315
                     /locus_tag="VF589_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257027.1"
                     /translation="MIRIATLAASLLLAAGCGGREPLRPAPGQSMPVAPAMAAAAPTT
                     ADLLTPPPIARPQRVDELLRRSEEREDDRFDLPPQ"
     gene            9338..10198
                     /locus_tag="VF589_05300"
     CDS             9338..10198
                     /locus_tag="VF589_05300"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX8257028.1"
                     /translation="MKPVRKAVFPVAGLGTRFLPATKAFAKEMLPIVDRPLIQYAVDE
                     AREAGIEQLIFVTGRGKGALEDYFDIAYELEATMLARGKSLDALAPTRLPAGALASVR
                     QQEPLGLGHAVWCARDIVGDEPFAVILPDDLMWGRPGCLRQMVEAYEEVGGNIICVEE
                     VAAEKTASYGIITPGASRGRLTEVKGLVEKPSPETAPSRLGIIGRYILQPEVMRVLDN
                     PTKGAGGEIQLTDAMAALIGDQPFHGVTVDAVRYDCGDKAGFVTANIALALERDDVAP
                     AVRAFIADLR"
     gene            10199..11152
                     /locus_tag="VF589_05305"
     CDS             10199..11152
                     /locus_tag="VF589_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HEX8257029.1"
                     /translation="MTCRALLVGDGAIRKVSPDEAASYAGPGFVWIHVDGADETDLAL
                     LRGNGIPDIAANALVATETRPRCDRIEDGALVNLRGPAEIDTEDSDRLVSIRFWVRQG
                     KVNSVTRRPLAATATVIAQMEGARIHDPGDLVAAFARAISKQLDPQVADLGDRLDDCE
                     SGLEPRQVYKLRIMIAQIRSEAISFRRFVAPDRDALSTLAHIDFDWLAEEDRLHIREA
                     ADRFARMTEELEAVRERAALLHEQLTDMRAEQLDSRSLVLSIVALIFLPLTFITGLLG
                     MNVAGIPYADEPWAFWAIVGLCVLIGLAVMAYFARIHWLNR"
     gene            complement(11209..11284)
                     /locus_tag="VF589_05310"
     tRNA            complement(11209..11284)
                     /locus_tag="VF589_05310"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11249..11251),aa:Phe,seq:gaa)
     gene            complement(11329..11523)
                     /gene="yacG"
                     /locus_tag="VF589_05315"
     CDS             complement(11329..11523)
                     /gene="yacG"
                     /locus_tag="VF589_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX8257030.1"
                     /translation="MSRAASPDPRRQKCPVCGKPPSGAHGPFCGQGCRDRDLLKWLGD
                     DYRVPGPPADPFSTDDGLDS"
     gene            complement(11501..12493)
                     /locus_tag="VF589_05320"
     CDS             complement(11501..12493)
                     /locus_tag="VF589_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEX8257031.1"
                     /translation="MPEWLYEEGIGENRAALVADGTILEAAIELPGLLSAGSVVDGRL
                     AAILIPGRRGIVRLREGGEALLEPLPAGLTEGRAVRVEIVREAISESGREKLPKCRLS
                     EAEPRLGPTLIERIGRGGEPVTVLAAHQRDRLEEAGWSELIEQAMVGDFLFEGGTLRM
                     SLTPAMTLFDVDGALPPPALAIAGAAAAARAVRRLGIGGSIGIDLPTLPAKADRQAAA
                     AALDAALPQPFERTAVNGFGFLQLVRRRERASLPELLQGDPAGAAARALVRKAERSHG
                     SGERTLRAAPVVIARIERHPEWIDALARRLGAPVALQAEPGLAISAGHVQSRFA"
     gene            complement(12601..>12750)
                     /locus_tag="VF589_05325"
     CDS             complement(12601..>12750)
                     /locus_tag="VF589_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX8257032.1"
                     /translation="EWRGKAGGYAIQGRAEALVKAIAGSHSGVVGLPLYETRALLRAA
                     GYPVG"
BASE COUNT         2055 a         4485 c         4270 g         1940 t
ORIGIN      
        1 aatacgggcc agttcgagcg cacccttatc gtcgccgaca agggcagcta cgtctcctac
       61 ctcgaaggct gcaccgcgcc gatgcgcgac gagaaccagc tccacgccgc agtggtcgag
      121 ctgatcgcgc tcgacgatgc cgagatcaaa tattcgaccg tccagaactg gtatccgggc
      181 gacgccgaag ggcggggcgg catctacaat ttcgtcacca agcgcgcgat gtgcgccggc
      241 gaccgctcga aggtaagctg gacccaggtc gagaccggct ccgccatcac ttggaaatac
      301 ccctcctgca tcctgaaggg cgagggtagc gtcggcgaat tctattcggt cgcgctcacc
      361 aacaacatgc agcaggccga caccggcacc aagatggtcc acatcggcaa gaacagccgc
      421 tcgaccatcg tctccaaggg catcagcgcc ggccgctcca acaataccta tcgcgggctc
      481 gtccgcgtca atgcgggggc ggagaacgtc cgcaacttca cccagtgcga cagcctcctg
      541 ctcggcgacc agtgcggcgc ccacaccgtc ccctatatcg aggtaaagaa ccccaccgcc
      601 accatcgagc atgaggccac cacctccaag atcagcgacg accagatgtt ctacgcccgc
      661 cagcgcggat tgaacgccga agacgccgtc gccctcatcg tcaacggctt cgccaaggag
      721 gtgctgcagc aactcccgat ggagttcgcg gtggaagcgc agaagctgct gggcatcagc
      781 ctcgagggaa gcgtgggatg agtcgggaca ggcttgatcc ggcacagctc gaagcagcgc
      841 acaaacactc gatcttccat cgtaaggagg tcgaagcaag cgaaaccttt ggctgctttc
      901 actgtaagaa atctggcgcc gtggctgaaa tcgctgaatg gaccgatgac ggcgacacgg
      961 cgatctgccc taagtgcggc atagactccg ttgtcgggtc tgcctccgga tttcctgtta
     1021 caaacagcct tttccttggg gctatgaaga gacgttggtt ctgacatgct gcgaatcgaa
     1081 aacctccacg ccgagatcga cggcaagcag atcctcaacg gcctcacgct cagcgtcggc
     1141 gccggcgagg tgcatgcgat catggggcca aacggcgccg gcaagtcgac gctcggctac
     1201 gtgctcggcg ggcggcccgg ctatgaggtg accgagggct cggtcacctt ggacgggcgc
     1261 gacctgctcg acatggagcc ggacgagcgc gccgccgctg gcctgttcct cggcttccag
     1321 tatccggtcg agattcccgg cgtgtccaac gttcagtttc tgcgcaccgc cctcaacgcc
     1381 cagcgccgcg cgcgcggcga ggcggaggcc tcggcgggtg attttctgcg gctggccaag
     1441 gagcagggcg cggcgctcga cgtctcgatg gacatgctga agcgcaacgt gaatgtcggc
     1501 ttttcgggcg gcgagaagaa gcgcaacgag atggtccaga tgggcatcat cgccccgcgc
     1561 ctcgccgtcc tcgacgagac cgactcgggc ctcgacatcg acgcgctgcg catcgtcggc
     1621 gagggcatca accggatcat gcgcgcgccc gacaaggcgg tgctcctcat cacccattat
     1681 cagcgcctgc tcgacctcgt cgagccggac ttcgtccacg tgctggcgga aggcctgatc
     1741 gtgcggtcgg gcggccccga gctcgcccat ctgctcgagc gcgaaggcta tggagcgatc
     1801 gcggcgtgac cggaaccggc ccccttccac gtcacgggca ttacggaatg ccccacgcag
     1861 gtggcgcacc cgcgcgcgcg cgcgcgatac acactgactt tccagacctt cgtccgattc
     1921 cggacagccg tcgcgttctc gggagcccgg cgacatgaac gttctccacc tcccttcgaa
     1981 tcgcaccgag gcttggcgct ggagcgattt gtcggcgctg ccggagatcg ccgcgcgcca
     2041 gccagctggc accgtgcccg aggcgctgcc ctggatcgcc tgcgccaacg agggcccgcg
     2101 tctgctgttc gtcgacggcc gcctcgccgc cgagcggagc aacctcggca ccatcgaaat
     2161 cggcgcgatc gagcccgagc cgcgcgacca tgcgctggcc cgccaggtcg gccgcgaagg
     2221 ctggaagctg aagctcggcc gcgaccatgc gccgcagggg ctggtgcaga tcgtccacgt
     2281 ctctaccggc gccgccgatc acctccccgc cgaggtcgag ctcgacgtcg atgcgcaggc
     2341 gagcatcgtc gagaccttca tcggccacgg ctggaccaac cgcttgagcc atttccgcct
     2401 gttcaagggc gcgcggctga tgctggcgcg gcggctgatg agcgatgcgg gcccttcggc
     2461 ttcgctcagg acaagcttca tcagcctcac cgaccgcgcg gtagtcggcg agggcgccag
     2521 cttcatcctc accacgctcg cggccggcgg cgccgacacc cggctcgacg gcgaggtccg
     2581 gatcgccggc gaggccggct tcgccgaagt cggcggcgcc ctgctggcgc gcggacgggg
     2641 acgccatgac gccaatctgg tcgtccgcca cgaggcggtc ggcggggtca gccggcaggt
     2701 gtggcgctcg gtcgcggacg accgcgcgac ctgctcggtg gcggcgcgcg tcgaagtcgc
     2761 ccgggcggcg cagaagaccg acggagagca gtcgctcaaa ggcctgctgc tggaccgcgg
     2821 cgccaccatc aacgccaagc ccgagctcga aatcttcgcc gacgacgtca aatgcgccca
     2881 cggcgccacc gtcggcgaat tgaaccgcga cgcgctcttc tacctcgcca gccgcggcgt
     2941 tcccccgcag gaagcgaaag cgctcctcac ccaggccttc gtcgccgacg ccatcgaccg
     3001 caccggcgag ccagccgtgc gcgacgcctt ccacgccgat gccctggcgt ggctgggagg
     3061 aactcgatga gcacggacgc ccttaatacc ccctctcccc agcggggaga gggctggggt
     3121 gagggggagc gagcgcagcg agcgtccgac ccccgagcct tgcctccgac tcagagcccc
     3181 ctcacccaac cctctccccg ccggggagag ggctcaaata cccgacccct cgacctggtc
     3241 tccgatttcc ccgccatccc ctccggctgg gcctatctcg acaccgccgc caccgcccag
     3301 aagccgcggc cggtgatcga cgcgatcgcg cgcggctatg ccgagaccta tgccaccgtc
     3361 catcgcggcg tctatcagcg ctcggccgag atgacgctcg ccttcgaagc cgcccggcac
     3421 ggcacggcgg cgttcatcgg tgcgcctgtg ccggaggaag tgatcttcgt gcgcggcgcg
     3481 accgaaggca tcaacctggt cgcgcaaagc tggggcggca ccttcctgaa gcccggcgac
     3541 cgcatcatgc tgtcgatgct cgaacatcat tccaacatcg tcccctggca gctcgtcgcc
     3601 gagcgcaccg gcgccgagat cgacgtcgcg ccgctgaccg cggacggctg catcgatctc
     3661 gacgcgatgg cggcgatgct gaccgagcgg cacaaaatgg tggcgctggc ccatgtctcc
     3721 aacgtgctcg gctcggtgct cgacgcccgc cgcgcggccg atctggcgca cgatgtcggc
     3781 gccctgctcc tgctcgacgg ctgtcaggcc gtgccgcgca tgccggtcga cgttgccgca
     3841 ttgggctgcg acttctacgt cttctccgcc cacaagcttt acgggccgac cgggatcggc
     3901 gtgctgtggg gcagaggcga tctgctcgac gcgatgccgc cctggcaggg cggcggcgca
     3961 atgatcgaca aggtgagctt cgaaggcacc actttccttc ccccgccctc tcgcttcgag
     4021 gcggggacgc cgcacgtcgt cggcgtgctc ggcctccacg ccgcgatcga ttatgtgcga
     4081 gccatcggcc tcgatgcgat cgccgcgcac gaggccgcgc tggtgcgcgc gacccgcgac
     4141 gcgctgtcgg cgatcaacag cctgcgcctg ttcggccccg acgacagcgc cggcatcgtc
     4201 agcttcgccg tcgagggggt gcacccgcac gacgtcggca ccatattgga cgagagccgg
     4261 gtcgcgatcc gggccgggca tcattgcgcc cagccgctga tggcgcatct cggcgtggac
     4321 gccaccgccc gggcgagctt cggcgtctac aatgatagac aggatgtcga ggccctggtg
     4381 cggggcatcg agcgagtgac gagaatcttc ggatgaacga ggaacgcaag atcgagatcg
     4441 aggaagtgag cgaggcgcca aagcctccca aggcgcgcgt cgctcctccg ggggccacgg
     4501 agggcgaccg ggcggaggct ggcggaggcg gctcgccgct ctcgcaggcg ttcgagcgca
     4561 agaaggacta tctcgccggc ttcctcgccc acaagcccga ggaggcggct cccgtcgagc
     4621 ctggcggcaa cctctacgag gccgtgatcg aggcgctgaa ggagatttac gacccggaga
     4681 tcccggtcaa catctacgat ctcggcctga tctacgacgt cgagatcacg cccgaacacc
     4741 acgccatggt caagatgacg ctgacgacgc cgcactgccc ggtcgccgaa tcgatgcccg
     4801 gcgaagtcga gctccgcgtc ggctccgtcc ccggcatcgg ccatgccgaa gtcgaactgg
     4861 tctgggatcc gccctgggac ccgcagaaga tgaccgacga ggccaagctc gaactgggca
     4921 tgctgtgatg ggcttgacac taggccgttc gtcccgagcg cagtcgaggg accctgccga
     4981 gcgaagccga ggcttagctt ctcgcctagg ctcgaagggg tgtctcgact tcgctcgaca
     5041 cgaacgggaa ggtttcatca gatgagcacc accacccgcg cccgccctgc ggcgctgaac
     5101 ctcacgcccg cagccgaggc ccggatcgcc gcgctcatgg cccgtgcgcc ggaaggggcg
     5161 atcggcgtca agctctccac cccgcgccgc ggctgctccg gcctcgctta ttcggtcgac
     5221 tatgtcaccg aggagaagcc gttcgacgag aagatcgaga ctccgggcgg catcttctat
     5281 gtcgacggcg cctcgatcct ctacctgatc ggcagccgca tggactggaa agaggacgat
     5341 ttcgctgccg gcttcgtgtt cgacaatccc aacgccaagg gcgcctgcgg ctgcggcgag
     5401 agcttcaccg tctgacgccc cgcctggccg gctgagccag tccgatccgg attgcgacca
     5461 gccgagggca ggcctcagca actaaaagga atcagcgggg tttcttcttc aacaaaggtc
     5521 gggcggcggg ctgttggaga gccattatgt tgaagccctt gcttgcgtgc atgctgctgg
     5581 cggccccggc cgccatttcc gccgcccaga cccccgcggt tcaggccccg acagcgcccc
     5641 aggccgcgcc gggtgagtgg accgtctccg gcagcgcgca aggctgcatc ctctacggtt
     5701 cgtcggcgca agggacggtg gtgtcgatcc tcgccggccc ggggcaggac gcgctcatct
     5761 tcctcgtcca aaaccggcaa tggtcgtcgc tccaggacgg cagcaagcat gcggtggcgg
     5821 tggcgttcga cgggcgcaac gagtggaatt tcgaagcggt cgccaaaacc gagctcgaca
     5881 gcgacgggcc gggcctgctg ttcgcggtca agcccggcga aaaggagggc gtcaacttca
     5941 tcacccagtt cgccgccgcc aacggcatgc gcatcgaccg cgacggccag cagctggcga
     6001 gcctcccggt gggtgacggc aacacggcca tggccgcgct cgcccagtgc atgggcaaga
     6061 tgtattcggg cgcgtccgaa gccgcgggcg aggaatcgac cccggtcttc cagacaagct
     6121 cggaaaaacc ggtcagcctt tagactcccc tgcgccggcg tgcgtgagcc acgcgcgccg
     6181 gccgcttcca atgtcagttc tcctgcggaa cgacccttgc gtcagcgagt tttatcaggc
     6241 gtatatcgtg cgcccgcagg aggctcgcgg gcggcggttg ggcgtggcgt cgcgtggaga
     6301 agggcttgtt aaccattccg atccagactg agccttcccg ccggagcggc cggcggcggc
     6361 gccgttgagc gcgagggacg ggaggccagg gacatgacga gcaacgactt catccggcag
     6421 ctccagggct acgggctcac caccgccgag atcagctatt acatgcccga ccaccccaag
     6481 ctcatccaat tgttcgtctg gcagacctat gacgtggcgc ccacctttcc cgtgctccac
     6541 ggcttcctcg accattggcg ccgcgagatc gaagcggcgc tccattcggt gcgggtggcg
     6601 caccagcatc tgatccgccc cgccgaatgg cgcgccgtcg acggggtgat ctccatccac
     6661 tgagttccca gtgatctgtt ccccggccga aagccggggt ccaggacgaa ctcccaactg
     6721 ggccccggcc ttcgccgggg aacgcgatcc agcccgaccc cggcttccgc cgcggcacag
     6781 gcgcgactaa gggacggcga tgcaacccgc cctcgcccat atcgacgcct ggatcttcga
     6841 cctcgacaat tgcctctacc cggcctcgac cgacctgttc gcgctgatcg acgtgcgcat
     6901 gggcgagtat atcgccaggc tgctcggcgt cgacgcggcc gaggcgcggc gggtgcagaa
     6961 actctatttt cacgaacatg gcaccacgct ggccgggctg atggcggcgc acggcgtcga
     7021 tccgcacgag ttcctcgatt atgtccacga catcgacctt gcccgcctcg ccgccgaccc
     7081 cgccttggtg gcggcgctcg accggctgcc gggccgcaag ttcgtcttca ccaatggcga
     7141 cgaggcctat gccggccgcg tcctcgcccg gctcggcctc gccgacgcgt tcgacggcct
     7201 gcacgatatc cacgccatgg actatctgcc gaagccgaac ccggcttcct atgcagcctt
     7261 gtgcgagcgg cacggcatcg cgcccgagcg cgcgctgttc gccgaggaca tggcgcgcaa
     7321 cctcgcgccg gccaaggcgc tggggatgac gacggtctgg gtcgacaacg gctccgagcg
     7381 cggcagccac ggcgccgacc aaagctttat cgactatcgc gtcgccgata tcggcgactg
     7441 gctgaccgaa atcacggggg acgaagcatg accgaggaac tgcagcatgt gatcgaggac
     7501 gcctggctgc ggcgggaatc gctcgatccc tcgacctcgg ggccggtgcg gcaggcggtg
     7561 gtcgccgcca tcgccgcgct cgacgcgggc cgggtgcggg tggccgagaa agccgagggc
     7621 ggctggcagg tcaatcagtg gctcaagaag gcggtgctgc tgagcttccg cctcaatccc
     7681 atggaagcga ttgccggagg cccgggcggc gcgccctggt gggacaaggt cccctccaag
     7741 ttcagcggct gggacgcggc ccagttcgag caggccggat tccgcgcggt gccgggcgcg
     7801 atcgtccgcc gcggcgcctt cattggccgc ggcgcggtgc tgatgccgag ctttgtcaat
     7861 atcggcgccc atgtcggcga ggggacgatg gtcgatacct gggcgaccgt cggcagctgc
     7921 gcgcagatcg gccgcaacgt ccacatatcg ggcggcgccg gcattggcgg cgtgctcgag
     7981 ccgctgcagg ccggcccggt ggtgatcgaa gacggcgcct tcatcggcgc ccgctcggaa
     8041 gtcgccgaag gcgtgatcgt cgaggaaggc gcggtgctgt cgatgggcgt gttcctcggc
     8101 gcctcgacca agatcgtcga ccggatgacc ggccaggtct tcatgggccg cgtcccggct
     8161 tattcggtgg tggtgcccgg ctcgctgccg cccaagtccg gcgacggacc ggcgctcgcc
     8221 tgcgcggtga tcgtgaagcg ggtcgacgcg cagacgcggt ccaagaccag catcaacgag
     8281 ctgctgaggg actgaggaag ggtttgcggg atcactctac ccgctcatcc tgaggaggga
     8341 ctgagccttg gcgaaggccc gtctcgaagg acctggtgcg tccttcgaga tgccgcttcg
     8401 ccaagctcag cggctcctca ggatgagcgg agtgcccccc gcgctactcg acccccgcct
     8461 ccctcagcaa cccggccgcg cgcgcgccgg tccaatcctc gatccccgtc atccgccaca
     8521 cctcgcggcc ctcggcgtca tacaggatgg tggtcggaag cgtcagcacc ttgagttcgc
     8581 ccatcagcgc catttccgta tcgagatagg gttcgagcgc ctcgaacttg cgcgccgcga
     8641 agaaggcgtc gaccttcttg gcgccctcaa tatcctggct gacggcgagc acttcgagat
     8701 tgccgccctt cgctgcgagc gcgtccagcg tcggcagttc gacgatgcac ggcgcgcacc
     8761 aggtcgccca gaaattgacc agcagcggct ttccgcgaaa gtgggcgagg gtgaccggct
     8821 cgcccgccgg atcctcgaag ctcgcctccg gcgcgcgcgt gccggccttg gagcggtcga
     8881 ctttgccccg cggcaggtcg aggtcggcgg ctacgaccgg cgccgggtcc gacgcggccg
     8941 gctggcgttc gcaggcggcg agggggagga ggaggggcaa tgccaggagc agtcgcacgg
     9001 ccggctgggt agaggccggc ggcggcggcg acaaggaggc ttcgcatttc gccgccacgc
     9061 cggtagaagc ggccgatgat caggatcgcg accctcgccg cctcgctcct gctcgcggcg
     9121 ggatgcggcg ggcgcgagcc gctgcggccc gcccccggcc agagcatgcc ggtcgcgccc
     9181 gccatggcgg cggcggcgcc gaccaccgcg gatctgctca cgccgccgcc gatcgcgcgg
     9241 ccgcagcgcg tcgacgagct gctgcgccgc tcggaggaac gcgaggacga ccgcttcgac
     9301 ctgccgccgc aatagccgca aaattaccgg agagaccatg aaacccgtcc gcaaggccgt
     9361 cttccccgtt gccggtctcg gcacccgctt cctgccggcg accaaggcct tcgccaagga
     9421 gatgctgccg atcgtcgacc ggccgctcat ccaatatgcg gtcgacgagg cgcgcgaggc
     9481 cggcatcgag cagctgatct tcgtcaccgg ccgcggcaag ggcgcgctcg aggactattt
     9541 cgacatcgcc tacgagctcg aggcgaccat gctcgcgcgc ggcaagtcgc tcgacgcgct
     9601 ggcgccgacg cggctgccgg cgggcgcgct cgcctcggtg cgtcagcagg agccattggg
     9661 cctcggccac gcggtctggt gcgcgcgcga catcgtcggc gacgagccgt tcgcggtgat
     9721 cctgcccgac gacctgatgt ggggccggcc cggatgtctg cggcaaatgg tcgaggctta
     9781 tgaggaagtc ggcggcaaca tcatctgcgt cgaggaggtc gcggccgaaa agacggcgag
     9841 ctacggcatc atcacgccgg gcgcgagccg cggccggctg accgaggtca aagggctggt
     9901 cgagaagcca agccccgaga ccgcgccgtc gcggctgggc attatcggcc gctacatcct
     9961 ccagcccgaa gtgatgcggg tgctcgacaa tccgaccaag ggcgccggcg gcgagatcca
    10021 gctcaccgac gcgatggcgg cgctgatcgg cgatcagccg ttccacggcg tcaccgtcga
    10081 tgcggtccgc tacgattgcg gcgacaaggc cggcttcgtc accgccaata tcgcgctcgc
    10141 gctggagcgc gacgacgtcg cgccggcggt gcgcgccttt atcgctgacc tgcgctgaat
    10201 gacctgccgc gccctcctgg tcggcgacgg cgccataagg aaggtatcgc cggacgaggc
    10261 ggcgagctat gccgggcccg gcttcgtctg gatccatgtc gacggcgccg acgagacgga
    10321 tctcgccttg ctccgcggca acggcatccc cgacatcgcg gccaacgcgt tggtggcgac
    10381 cgagacgcgg ccgcgctgcg accgcatcga agacggcgcg ctggtcaatt tgcgggggcc
    10441 ggccgagatc gacaccgagg atagcgaccg gctcgtctcg atccgcttct gggtccgcca
    10501 gggcaaggtc aattcggtga cccgccggcc gctggcggcg acggctaccg tcatcgcgca
    10561 gatggagggg gccaggatcc acgatccggg cgatctcgtc gcagccttcg cgcgcgcgat
    10621 cagcaagcag ctcgatccgc aggtcgccga cctcggcgat cggctcgacg attgcgaaag
    10681 cgggctggag ccgcgccagg tctataaatt gaggatcatg atcgcccaga tccggtccga
    10741 ggcgatctcc ttccgccgct tcgtcgcgcc cgaccgggac gcgctttcga cgctcgcgca
    10801 tatcgatttc gactggctgg ccgaggagga ccggctccac atccgcgagg ccgccgaccg
    10861 cttcgcccgc atgaccgagg agctggaggc ggtccgcgag cgcgcggctt tgctgcacga
    10921 gcagctcacc gacatgcgcg ccgagcagct cgattcacgc tcgctggtgc tgtcgatcgt
    10981 cgccctcatc ttcctgccgc tgaccttcat caccggcctg ctcggcatga acgtggccgg
    11041 cataccctat gcggacgagc cgtgggcgtt ctgggcgatc gtcggcctgt gcgtcctgat
    11101 cggcctcgcc gtcatggcct atttcgcccg catccactgg ctcaaccgct aagctcccgc
    11161 cctcgtggcg gtggggcttt ccggatcagg acgtcggggg aaggataatg gtgcccaggg
    11221 acgggatcga accgccgaca ccgtgatttt cagtcacgtg ctctaccaac tgagctacct
    11281 gggcataccg ccgccggcgg aagtcagcgc ctatagaggc ggcccgcctt agctgtccag
    11341 cccgtcgtcc gtcgaaaacg ggtcggccgg gggaccgggc acgcgatagt cgtcgcccag
    11401 ccacttcaac aggtcgcggt cgcggcagcc ttggccgcag aacgggccgt gggcgccgct
    11461 cggcggtttg ccgcagaccg ggcatttctg gcggcgggga tcaggcgaag cggctctgga
    11521 catggccggc cgatatggcg agccccggtt cggcctgcaa ggcgacggga gcgccgagcc
    11581 gccgtgcgag cgcgtcaatc cactccgggt ggcgctcgat ccgggcgatc accaccggcg
    11641 cggcgcggag cgtgcgctcg ccggagccgt gggatcgctc tgccttgcgc accagcgccc
    11701 gcgcggccgc gccggccggg tcgccctgca gcaattctgg gagcgatgcc cgctcccgcc
    11761 gccgcactag ctgcaggaag ccgaagccgt ttacggcggt tctctcgaac ggctgcggca
    11821 gcgccgcgtc gagcgccgcc gccgccgcct gccggtcggc cttggcggga agcgtcggca
    11881 ggtcgatgcc gatcgagccg ccgatgccga ggcggcggac ggcgcgggcc gcggcggcgg
    11941 ccccggctat tgcgagcgcg ggcggcggca gcgcgccgtc gacgtcgaac agggtcatgg
    12001 cgggggtaag gctcatccgc aacgtcccgc cttcgaacag aaagtcgccc accatcgcct
    12061 gctcgatcaa ctccgaccaa cccgcttcct ccagccgatc gcgttggtgg gccgccagca
    12121 ccgtcaccgg ctcaccgccg cgaccgatgc gctcgatgag cgtgggccca aggcggggtt
    12181 ccgcctccga aagccggcat ttggggagct tctcgcgtcc gctttcggag atcgcctcgc
    12241 gcacgatctc cacccgcacg gccctgcctt cggtgagccc ggccggcagc ggctcgagca
    12301 gtgcttcgcc gccttccctc aggcgaacga tgccgcgccg gccggggatc aggatggccg
    12361 cgagccgccc gtcgacgacg ctgcccgcgc tgagcagccc cggaagctcg atggcggctt
    12421 cgaggatcgt gccgtccgcg acgagcgcag cgcggttctc gccgatccct tcctcgtaga
    12481 gccactcagg caatcggggt cccctcgatc aaggcgccgc atcgagtgtg tggcccctcg
    12541 tcatccgcgc tttcgcgggg atgacaatcc atagggcccg gccgcctcac gccgagttcc
    12601 ctaaccgacc ggatagccgg cggcgcggag cagggcccgc gtctcgtaga gcggcaggcc
    12661 gaccacgccc gaatggctgc ccgcgatcgc cttcaccaac gcctcggcgc gcccctgaat
    12721 cgcatagccg ccggccttgc cgcgccattc
//
LOCUS       DASVRK010000137         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_461174, whole genome shotgun sequence.
ACCESSION   DASVRK010000137 DASVRK010000000
VERSION     DASVRK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461174"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VF589_05330"
     CDS             complement(<1..789)
                     /locus_tag="VF589_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8257033.1"
                     /translation="MDEQSPSLTPARIARRLLLAAVLAGLWITAYAYGEAIAEPVVRR
                     AQVKLAGWPAGAAPVTALFISDVHVGGPDMPPERLARIVAQINRLRPDLVLIAGDLIS
                     DKRAATHHYTMAEALAPLGGLRPRLGTVAVLGNHDYWHDAPAARAGLAKAGVKLLHNE
                     GLAIGPLAIGGLDDDFTRHADLPGTLERMRPIPGGRLLLSHSPDPFASLDADVSLMLA
                     GHTHCGQIAPPPFGAIETMSRYGKRFACGVIRERGRTLIVTAGLG"
     gene            974..1858
                     /locus_tag="VF589_05335"
     CDS             974..1858
                     /locus_tag="VF589_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8257034.1"
                     /translation="MATIAHLSDVHFGRHDPAIVEAVETYLFARRPDLVVISGDFTQR
                     ARVAQYQLASEFVERLEQGGLDILAVPGNHDVPLYDVVRRFVRPLHRYKRFIDDDLCP
                     WFENDQLAVLGINTARSLTIKDGSVSLEQLANIRSSFRDVAADKTRILVTHHPLFAMP
                     IGDEGELTKVVKRHTDALSAVAEAGVQLLLAGHFHRAFASSAREMVETAGPSLVIQAG
                     TATSTRLRADELQSFNWIETAPQRVELRVERWDGRAFVAGRPTWFEYDGTNWAYAGRR
                     EDIGTPARQLAAAASEPG"
     gene            1969..2148
                     /locus_tag="VF589_05340"
     CDS             1969..2148
                     /locus_tag="VF589_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257035.1"
                     /translation="MTKSGKSERFDEIEQTQANLRQNIEASKKLIERSDDLLENYRRL
                     ASRTPQPPPPQEAGC"
     gene            complement(2461..>2629)
                     /locus_tag="VF589_05345"
     CDS             complement(2461..>2629)
                     /locus_tag="VF589_05345"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543946.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8257036.1"
                     /translation="NVNGGAIAIGHPIGASGARVLTTLLYEMGRRDAKKGLATLCIGG
                     GMGIALCVERD"
BASE COUNT          426 a         1014 c          803 g          386 t
ORIGIN      
        1 ccccagcccg gccgtcacga tcaacgtccg cccgcgctcc cggataaccc cgcaggcgaa
       61 gcgcttgccg taacgcgaca tcgtctcgat cgccccgaac ggcggcggcg cgatctgccc
      121 gcaatgggta tgccccgcca gcatcaggct cacgtccgca tcgagcgaag cgaacgggtc
      181 cgggctgtgg ctcagcagca gcctgccgcc ggggatcggc cgcatccgct ccagcgtccc
      241 cggcaaatcg gcatggcgcg taaaatcgtc gtcgagcccg ccgattgcca gcggcccgat
      301 cgccagcccc tcattatgga gcagcttcac gccggctttg gccagtcccg cccgtgcggc
      361 cggcgcgtcg tgccaataat cgtggttgcc gagcaccgcc accgtgccga gccgcggcct
      421 cagtccgccc agcggcgcca gcgcttcggc catcgtataa tggtgggtgg cggcgcgttt
      481 gtcgctgatg aggtcgcccg cgatcagcac caggtcgggc cgcagccggt tgatctgcgc
      541 cacgatccgc gccagccgct cgggcggcat gtccgggccg ccgacatgca cgtccgatat
      601 gaacagggcc gtcaccggcg ccgccccggc cggccagccg gcgagcttga cctgcgcccg
      661 ccgcaccacc ggctcggcga tcgcctcgcc ataagcgtaa gcggtgatcc acagcccggc
      721 caggaccgcc gccagcagca gccgccgcgc gatcctggcg ggggtgaggg aaggggattg
      781 ctcgtccatc gccgctcagt tccacagcct cgcttaatat tccaactctc tccgacttcg
      841 aaccggtgca ccgcgttcgc gcgcagcctg agcggcccgc cgcgcacccc ggcaaccctg
      901 aaccgccctg tacgttcacc gggggccgag ggggcgatcc tccacccccg cttcgtgtcc
      961 gaaggagttc cggttggcga ccattgccca tctgtccgac gtccatttcg ggcgccacga
     1021 tcccgccatc gtcgaggcgg tcgagaccta tctgttcgcg cgccggcccg atctcgtcgt
     1081 catcagcggc gacttcaccc agcgcgcgcg cgtcgcccaa tatcagctcg cctccgaatt
     1141 cgtcgaacgg ctggagcagg gcgggctcga catcctcgcc gttcccggca atcatgacgt
     1201 cccgctttac gacgtcgtcc gccgcttcgt gcggccgctg caccgctaca agaggttcat
     1261 cgacgacgat ctctgcccct ggttcgaaaa cgaccagctc gccgtgctcg gcatcaacac
     1321 cgcgcgctcg ctgaccatca aggacggcag cgtcagcctc gagcagctcg ccaatatccg
     1381 ctcaagcttc cgcgacgtcg ccgccgacaa gacccgcatc ctcgtcaccc accatcccct
     1441 gttcgcgatg ccaatcggcg acgagggcga gctcaccaag gtcgtcaagc gccacaccga
     1501 cgctttgtcc gcggtcgccg aggccggcgt ccaattgctg ctcgccggcc atttccaccg
     1561 cgccttcgcc agctccgccc gcgagatggt cgagacggcc gggccgtcgc tggtgatcca
     1621 ggccggcacc gccacttcca cgcggcttcg cgccgacgaa ttgcagagct tcaactggat
     1681 cgagacggcg ccgcagcggg tcgagctccg cgtcgagcgc tgggacggcc gcgccttcgt
     1741 cgccgggcgg ccgacctggt tcgaatatga cggcaccaac tgggcctatg ccggccgccg
     1801 cgaggatatc ggcacccccg cccgccaact cgccgccgcc gcttccgagc ccggctgaac
     1861 cggcgctgtt gcagccgcgc aacgttcccg gaatagtgcg cgcgtcatga aaccggcgcg
     1921 cggcaacgcg gttaatgtcc cggtgggcaa aaaccgggtt gtgtaggcgt gacgaagagc
     1981 gggaagagtg agcggttcga cgagatcgag cagacgcagg cgaatctcag gcagaatatc
     2041 gaagcgagca agaagctgat cgagcggtcc gacgacctcc tcgaaaacta ccgccgcctc
     2101 gcgagccgaa ctccccaacc cccgcccccg caggaagccg gctgctagcc ctccccgacg
     2161 gccggcaagc gcgccgaccg cgcccctgcg aaggcagggg cccagaccag gcgcagcctg
     2221 tcagacgagg gtacaggtcg cgccagtccg gcttcatccg ctcgacgctg gtgagcctcc
     2281 agagccgttt cacatatccg cccttcatcc accgagaatg accgcactgc cgccaaagcg
     2341 ccagcccggg cctggacccc tgccttcgca ggggaacagg tgccgcaccc gtcccccgcg
     2401 aacgccgggc cagttctctc aacccccagc tgggcccccg gctttcgccg ggctaccccc
     2461 tcaatcccgc tcgacgcaga gcgcgatgcc catgccgccg ccgatgcaca aggtggcgag
     2521 gcccttcttc gcatcgcgcc ggcccatttc gtacagcaac gtcgtcagca cccgtgcccc
     2581 cgacgcgccg atcgggtggc cgatcgcgat cgcgccgcca ttgacgttg
//
LOCUS       DASVRK010000138         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_398972, whole genome shotgun sequence.
ACCESSION   DASVRK010000138 DASVRK010000000
VERSION     DASVRK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398972"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF589_05350"
     CDS             <1..327
                     /locus_tag="VF589_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257037.1"
                     /translation="KERDAVAPSGFTRRYGNSIAARIGVVARDGLDACLRGQGEAAPA
                     EEPGKREARPKPKRTAPAPRPERAEDYLEAPDIGAPLEPEAPAEAPAAEPAPVPETEP
                     AELNGL"
     gene            complement(331..654)
                     /locus_tag="VF589_05355"
     CDS             complement(331..654)
                     /locus_tag="VF589_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX8257038.1"
                     /translation="MPDLNKLMEMAQNAQAELQKAQESLDQVEVEGVSGGGLVKIRAS
                     AKGRIIAVEIDESLLQPSEKQMVEDLVAAALNDARGKADLAAHSEMQKVTSGLPLPPG
                     FKMPF"
     gene            complement(667..2355)
                     /locus_tag="VF589_05360"
     CDS             complement(667..2355)
                     /locus_tag="VF589_05360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX8257039.1"
                     /translation="MATESLDTPEDGFGGLGLGEPPQPQKPAPSRAEGAQPYRVLARK
                     YRPQSFEELIGQDAMVRTLGNAIRRDRLAHAFLLTGVRGVGKTSTARLVAKALNCIGP
                     DGEGGPTISPCGVCEPCVAIAEGRHIDVVEMDAASNTGVDDVREIIEAVRYAAVSARY
                     KVYIVDEVHMLSKNAFNALLKTLEEPPAHVKFLFATTEVNKVPVTVLSRCQRFDLRRI
                     SAEQLAAHFADVAAREGAEVEREALALIARAAEGSARDGLSILDQAIAHGHGAVTADQ
                     VRSMLGLSDRGAIRRLLGLLLAGDAPAALAAVREQYDLGVEPAALLRGLLESVHGITR
                     AKVGGAADPAQSVEEREAYADWASRLGYALIHRLWQLLLKGLAEVNSAAMPLEAAEMA
                     LLRVIHASELPDPGTLMEKLASGEAVPARPAAAPAAAAAGGQGAALQAPALFSGLVDM
                     LAAEGKPHLAQQLHDYVGLVRYAPPELAYRPVKPLSGDFARDLAAALKALTGTNWTVG
                     ASDGEAEPSLLEQEKAAAARLRQSVLDAPMVKAAFEAFPDAELAGYTYNDQRSA"
     gene            2459..>3019
                     /locus_tag="VF589_05365"
     CDS             2459..>3019
                     /locus_tag="VF589_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257040.1"
                     /translation="MSRGFWLLAPAIAACTAAAAQPADRAAAPSVLETPSEGPLPPGT
                     PKPVEPSLPLPKRIDISVCADQAKRGAFLATRPEDDAFMRHTMERAKFRERLVTWKLD
                     RLKKSGKLSDDEVMKLAQSGLSDPAAEAGMAASMDLLSDMMADIEQVMRAEEAKDDAA
                     ACRAFVSMMEKFEKIGPVVDRQWNAME"
BASE COUNT          473 a          985 c         1103 g          458 t
ORIGIN      
        1 aaggagcgcg acgcggtcgc gccctcggga ttcacccgcc gctacggcaa ttcgatcgcg
       61 gcgcggatcg gggtggtcgc gcgggacggg ctcgatgcct gcctgagggg gcaaggcgag
      121 gccgcgcccg cggaggagcc cggcaagcgg gaggcgcggc ccaagccgaa gcggaccgcc
      181 ccggcgccgc ggccggagcg ggccgaggat tacctcgaag cgcccgatat cggcgcaccg
      241 ctggagccgg aagcgccggc cgaagcgccg gcggcggagc cggcgccggt gcccgagaca
      301 gagccggccg agctgaacgg cctttagggc ttagaacggc atcttgaagc ccggcgggag
      361 cgggaggccg ctggtgacct tctgcatctc gctatgggcg gcgaggtcgg ccttgccgcg
      421 cgcgtcgttc agggcggcgg cgacgagatc ctcgaccatc tgcttttcgg agggctgaag
      481 caggctctcg tcgatctcga cggcgatgat ccggcccttg gcgctggcgc ggatcttgac
      541 gagaccgccc ccggagacgc cctcgacctc gacctggtcg aggctctcct gcgccttttg
      601 cagctcggcc tgggcgtttt gcgccatttc catgagcttg ttgagatcgg gcatgttcgg
      661 catgggtcag gcgctccgtt gatcgttata tgtgtaaccg gccagttcgg cgtcgggaaa
      721 ggcctcgaag gcggccttca ccattggcgc gtcgagcacg gactggcgca gccgtgccgc
      781 cgccgctttc tcctgctcga gaagcgacgg ctcggcctcc ccgtcagatg ccccgacggt
      841 ccagttggtt cccgtcagcg ccttcagcgc ggcggcaagg tcgcgggcga aatcgcccga
      901 cagcggcttg accgggcggt aagcgagctc gggaggcgcg tagcggacga ggccgacata
      961 atcgtggagc tgctgggcga ggtgcggctt gccttcggcg gcgagcatgt cgaccaggcc
     1021 ggagaataaa gccggagcct ggagcgccgc cccctgcccg cccgcggcgg ccgcggccgg
     1081 agcggcggcc ggacgggcag gcaccgcctc gccgctcgcc agcttctcca tcaaggtgcc
     1141 ggggtcgggc aattcgctgg cgtggatgac ccggagcagc gccatttcgg cggcttcgag
     1201 cggcatcgcc gcgctattga cctcggccag ccccttgagc agcagctgcc acaagcgatg
     1261 gatgagggca tagccgagcc ggctcgccca atcggcataa gcttcgcgct cctcgacgct
     1321 ctgggcggga tcggcggcgc cgccgacctt ggcgcgggtg atgccgtgga cgctttcgag
     1381 caggccccgg agcagggcgg ccggttcgac gccgagatca tattgctcgc gcaccgccgc
     1441 cagcgcggcc ggcgcgtcgc cggcgaggag caggccgagc aggcgccgga tcgcgccgcg
     1501 atcggaaagg ccgagcatcg agcggacttg gtcggcggtg acggcgccgt ggccgtgggc
     1561 gatcgcctgg tcgaggatcg acaggccgtc gcgggccgag ccctcggcgg cgcgcgcgat
     1621 cagcgccagc gcctcgcgct ccacttcggc gccttcgcgg gcggcgacgt cggcgaaatg
     1681 ggcggcaagc tgctcggccg agatgcggcg aaggtcgaag cgctggcagc gcgacagcac
     1741 cgtcaccggc accttgttga cctcggtcgt cgcgaacagg aatttgacgt gggcgggcgg
     1801 ctcctcgagc gtcttcagaa gcgcgttgaa cgcgttcttc gacagcatgt ggacctcgtc
     1861 gacgatatag accttgtagc gcgccgagac ggccgcgtag cgcaccgcct cgatgatctc
     1921 gcgcacgtcg tcgacgccgg tattcgaggc ggcgtccatc tcgaccacgt cgatatggcg
     1981 gccctcggcg atggcgacgc agggctcgca cacgccgcag ggcgagatgg tcgggccgcc
     2041 ctcgccgtcc ggcccgatgc agttcagggc tttcgcgacc agccgagcgg tcgaggtctt
     2101 gccgacgccg cggacgccgg tgagcaggaa ggcatgggcg agccggtcgc gccggatggc
     2161 gttgccgagc gtccggacca tcgcatcctg cccgatcagc tcctcgaagc tttgcggccg
     2221 gtatttgcgg gcgaggacgc gatagggctg ggccccttcg gcgcggctcg gggcaggctt
     2281 ttgcggctgc ggcggctcgc ccaggccgag gccgccaaag ccgtcttcag gggtgtcgag
     2341 agattcggtt gccatcggcg cagactagcg gctccggccg ggactgtcga agcccgtcgc
     2401 acaaagcgct tgcccgatag aggaaggctg gtagcctgct gtccgagggg gaaaaatcat
     2461 gagcagaggt ttctggctgc tggcgccggc gatcgccgcg tgcaccgctg ccgccgcaca
     2521 gcctgccgat cgggctgccg caccaagcgt tttagagacc ccctccgagg ggccgctccc
     2581 gcccggtacg ccgaagccgg tcgagccgtc tctgcccttg cccaagcgga tcgatatttc
     2641 ggtttgcgcc gatcaggcaa agcgcggcgc gtttctcgcg acccgtcccg aagacgatgc
     2701 attcatgcgc cacacgatgg aacgcgcaaa atttcgcgag cggctggtga cctggaagct
     2761 ggaccggctc aaaaagtcgg ggaagctgtc cgacgacgag gtcatgaagc ttgctcaatc
     2821 aggcttgtcc gacccggcag cagaagcagg catggcggcc agcatggact tgcttagcga
     2881 catgatggcc gacatcgaac aagtgatgcg cgcggaggag gcgaaagacg atgcggctgc
     2941 ctgccgcgcc tttgtcagca tgatggagaa attcgaaaaa atcgggcctg tcgtcgaccg
     3001 gcaatggaat gcgatggag
//
LOCUS       DASVRK010000139        16077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_461650, whole genome shotgun sequence.
ACCESSION   DASVRK010000139 DASVRK010000000
VERSION     DASVRK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16077
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461650"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..710)
                     /locus_tag="VF589_05370"
     CDS             complement(189..710)
                     /locus_tag="VF589_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8257041.1"
                     /translation="MASHPSSTYRSLFVTGLQNAHAVENQALALIDRQLDRLVRYPEV
                     AERLRLHRRETEVQIGRLDEILSGLRERHSSLKDMAMNFMGNFAALSNVMAADEILKN
                     SFVNFAFENFEIASYRSLIALAEAGGFGAAAPLLEATLAEEQAMAAWIEQSLPDLTLK
                     YAALRQGGRVASH"
     gene            829..1398
                     /locus_tag="VF589_05375"
     CDS             829..1398
                     /locus_tag="VF589_05375"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8257042.1"
                     /translation="MNVRPPLTLPERAKFSAHDFWLLARSGAFDGYAKSELIEGEIWV
                     MSAIHSWHAKTMSRLNVALHSALLAAGSNLEIFVPVSIALSDDSVPEPDLAVAEDHDD
                     GPLPLAKVKLAIEVSDTTLEIDLGRKALLYAKHGVPEYWVVARQGACLYRHSSPQAGG
                     YVDKDKFALGERIEAASIEGLTVETGEIS"
     gene            complement(1439..1627)
                     /locus_tag="VF589_05380"
     CDS             complement(1439..1627)
                     /locus_tag="VF589_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257043.1"
                     /translation="MDYVLILLASLSAALFAYVLLSKRELQLRGEYDRFARYLADRGE
                     VPPPFEIWIHEHWRKPGQ"
     gene            complement(1984..3663)
                     /gene="ettA"
                     /locus_tag="VF589_05385"
     CDS             complement(1984..3663)
                     /gene="ettA"
                     /locus_tag="VF589_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX8257044.1"
                     /translation="MAAQYSFVMKNMTKTFPGVPKPILNNISLQFYPDAKIAIIGPNG
                     SGKSTLMKIMAGRDKEFTGEAWPGEHIRVGYLEQEPQLDPTKTVKENVMDGVRPVADM
                     MERFNEISTIMGDPPEDADFDALMTEMGELQEKIDAVDGWSLDNQLEIAMDALRCPPG
                     DSPIHNLSGGEKRRVALTKLLLEKPDILLLDEPTNHLDAESVQWLEKHLVDYAGNVIL
                     VTHDRYFLDNVVNWVLELDRGRYYIYESNYSGYLDKKAKRLAQEEREDEGRQKAIQEE
                     LDWIRRSPKARQTKSKARIKAFDELVEAQENRTPGKAQILIQVPERLGGTVIEANGLT
                     KAYGDKLLFEDLTFSLPPGGIVGVIGPNGAGKTTLFKLIRGEEQPDAGGVRIGETVRL
                     GYVDQSRDALDAKKNVWEEVSGGSDVFRFGKHEVSTRAYVGAFNFKGPDQQKKVGQLS
                     GGERNRVHMAKMLQQGGNVLLLDEPTNDLDVETLRALEDALENFAGCAVVISHDRFFL
                     DRLATHILAFEGDSHVEWFEGNFEAYEEDKRRRMGPEADRPHRMTYRRLTR"
     gene            3767..5164
                     /locus_tag="VF589_05390"
     CDS             3767..5164
                     /locus_tag="VF589_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8257045.1"
                     /translation="MKKLFAATGLLAAASIAALPCPAFAQAGSTDALREAALGDTLAW
                     EITEGLTTEVGPRLAGTEAEARARDWAVRRLTGLGFANVRVETFDMPVWVRGEERAEI
                     MSPFPQPLAVTALGNSGATRARGLEADVVGFDSLEALEAASDASVRGKIVFVSHAMTA
                     TQDGSGYGWFGRARREGPSIASRKGAAAIVIRSIGTDYHRNPHTGVQIWAEGVKPIPA
                     GALALPDAEQLQRILKRSAGRPVRMRLTLTPRNIGTRQSGNVIAEIPGSDPAAGIVLA
                     ACHLDSWDLGTGAIDDAAGCGIVASAARRAAAGGRPRRTIRVVWFGAEEVGLFGGRDY
                     AAKHRDEKHAVIAESDFGADRVWRFNSYVGTAAQPVVDRIARALSPLGIAKGRTDEAS
                     GSDISVLSATGVPTFELSQDGTRYFDYHHTPDDTLDKVDPEQLRQNVAAWTTMLAIAA
                     NAPEDFAAMPPPGGN"
     gene            complement(5195..6004)
                     /locus_tag="VF589_05395"
     CDS             complement(5195..6004)
                     /locus_tag="VF589_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX8257046.1"
                     /translation="MPARTASIIAAASAALLLAGAAPPAPAPIALWRLDCGRISGEET
                     GAPPWRKRLLPVGCYLIRNGGRYLLWDAGMSARLAGNPRPNMALDRTLVDQLAEIGIA
                     PDAIEYVGISHYHGDHTGQASKFPKAELLIGAGDLTALKRTPPPAGSSPDHLAPWISG
                     GAPVAALIEDLDVFGDGRVTMLMTPGHTPGHSALLVRLASGNVLLSGDLWQNHGDVLT
                     DAMPGFNTSRAETAASRERFRRLAYKLDATVILQHEAADIDKLPPFPQFAQ"
     gene            complement(6095..6730)
                     /locus_tag="VF589_05400"
     CDS             complement(6095..6730)
                     /locus_tag="VF589_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX8257047.1"
                     /translation="MPWDRNQMAARAAKELRDGYYVNLGIGIPTLVANNIPEGMTVTL
                     QSENGMLGIGPFPYEDEVDPDLINAGKQTISQLPSSSYFSSSDSFAMIRGGHIDLTVL
                     GAMEVAENGDIANWMIPGKMIKGMGGAMDLVAGVKKIIVVMEHVSKNGDPKFIPECTL
                     PLTGKNVVDMVITDLCVFHRPDHQSPFRLIELAPGVTAEEVAARTTARYDA"
     gene            complement(6730..6897)
                     /locus_tag="VF589_05405"
     CDS             complement(6730..6897)
                     /locus_tag="VF589_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257048.1"
                     /translation="MQVGIEEAKRRMDELIAASLAGEEVVFTRWRKPIARLDPLTAEE
                     KQQRRLQAGAQ"
     gene            complement(6909..7352)
                     /locus_tag="VF589_05410"
     CDS             complement(6909..7352)
                     /locus_tag="VF589_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257049.1"
                     /translation="MGKPITLEQLQKMTSKQRMVIYEHARNGDGADADNIIRLLFENS
                     LLDTAGGGLPREHRVIQEIEKVCRSDTGVEAAVSAAKRGEAPLAGVDPLIQSAVREYG
                     HFDTTTWAGGFVAEEIQRLGWRRKGQKKLPSNCVAKTAAFFVEPE"
     gene            complement(7353..8060)
                     /locus_tag="VF589_05415"
     CDS             complement(7353..8060)
                     /locus_tag="VF589_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX8257050.1"
                     /translation="MQKIYANAEAALEGLLFDGMTICAGGFGLCGIPERLIDAIQASG
                     VKDLTIASNNAGIDNEGLGKLLRTRQVRKMISSYVGENKEFERQYLAGELEVEFCPQG
                     TLAERCRAGGAGIPGFYTKTGVGTEVAEGKEHKEFDGETYILERGIRADLSIVKGWKA
                     DEAGNLVFRKTARNFNQPMATSGRICVAEVEEIVPVGSLDPDSIHLPSIYVKRLISGA
                     PYDKKIEFRTVRQREAA"
     gene            complement(8133..8435)
                     /locus_tag="VF589_05420"
     CDS             complement(8133..8435)
                     /locus_tag="VF589_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8257051.1"
                     /translation="MTKSEVAARLNEKQAWLPGKRVKIDFGAGQGAILLDGQANQVTE
                     EDADADTTIKVSWEDWQALAAGQLDGMTAFMQGKLKVEGDMSNAMQLQGVLAKLKA"
     gene            8630..10837
                     /locus_tag="VF589_05425"
     CDS             8630..10837
                     /locus_tag="VF589_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8257052.1"
                     /translation="MDAAEVIHEKFVAKVKAGNFPRRPTDVQPGDVGLGREAMAETFY
                     SQCVSRQMDRLSRRLQARGEGFYALGSSGHEKNAAVAEALRVDDMAFLHYRSNAFQIH
                     RSKQLPGQTPCWDMLLSFAASAEDPISGGRHKVIGSKPLNIPPQTSTIASHLPKAVGA
                     AFSIGIAKMLKLGETPLPRDAVVLASFGDASANHSTAQGAFNTAAWTAYQGSPMPIIF
                     LCEDNGIGISTRTPKGWIEASFRDRPALKYIRCSGLDMADAYRGAAEAAHYARTQRRP
                     VFLHMDCIRLYGHAGSDVQATYLTKAQIEADEERDPLLYSAALLVEQDVMSGRDIIEI
                     YGEAEATLNRIGEQAITRPKLTTSAAVTASLVPPKRPPARANTPSSEDRNALFARDRN
                     MMDKPQHMARLLSWTLADLMLAHDNIIVAGEDVGPKGGVYNVTAKLHERFGSCRVINT
                     LLDEQSILGLGIGAAHNDILAITEIQFLAYVHNAEDQIRGEAATLSFFSNGQYTNPTI
                     IRIAGLGYQKGFGGHFHNDNSLAVFRDIPGLVLACPSNGADAVGMLRECARLALEEQR
                     VIVFVEPIALYMTRDLHEEGDALWTSTYAAPGEAKPVRIGEVAVHGEGTDLAIVSYAN
                     GYYLSRQAEKILREQHGVKARVIDLRWLAPVAEESLLGAVAGCERILIVDECRKTGSQ
                     SEALMAVFAERAPGVDCRRITAEDSFIPLGRAATLTLPSRDSIVAAALEAVRG"
     gene            10830..11873
                     /locus_tag="VF589_05430"
     CDS             10830..11873
                     /locus_tag="VF589_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX8257053.1"
                     /translation="MADRLSALVVCPGRGTYGKAELGYLKRFHADKAELIASFDRLRA
                     EAGQPTISELDGAERYSMALHTRGDIASPLIFAASYADFLAIDRARFDVAAVTGNSMG
                     WYTTLACGGAVDAEHGFGIVDAMGRNSQAGEPGGQVLLTLVDEQWRPVPGLRESLLGL
                     VEAIDGRAEASLYLSIDLGGMLVFAGNEAGLAALLAEAPPTPGREPLRLVNHGPFHTP
                     LMQGSSDRAFELPAEWVKGPAIPMIDGRGHIWRPFASNPQALRHYTFATQILESYDFT
                     RAVQVAVREHAPDRIILLGPGDTLGGAIAQALIAIEWRGMRSKQDFQEMQTSASPILL
                     SMGREEQRALVAA"
     gene            11979..12848
                     /locus_tag="VF589_05435"
     CDS             11979..12848
                     /locus_tag="VF589_05435"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8257054.1"
                     /translation="MLKTMMRVLPVPLLLAAGAAAAQVDMTDDAAMRPKLEALAGAGN
                     AEAAYHLGMMHHLGANGAKKDPQRALELFRQAAEGGDPLGAYKLGSYYDGQGGGLVQP
                     DPELALKHKMVAAKAGYSLAQHDVAKLLYARGDTGEAVRWLEKSARQGYGESLQALAS
                     LYTGEGKVAKNMGKSLAYLVILQNSLKGKKASPKIQAWVKETRAKLSPAEIAQAEDVV
                     RGWKVKPSPLTLQALAGEETAKTLVAKTPAPAAADAEPAAAEAAPAEADAAPAEAGPA
                     PQAEPAPAGPDRA"
     gene            complement(12930..14897)
                     /locus_tag="VF589_05440"
     CDS             complement(12930..14897)
                     /locus_tag="VF589_05440"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX8257055.1"
                     /translation="MTDFAAAREKMVERQIAARGISDPRLLDAMRLVPREEFVPGHLK
                     EFAFDDAPLPIEAEQTISQPYIVALMIEAAELGGGDRVLEIGGGSGYAAAIMGQVAGE
                     VIAIERHAELVRLATERMERFGYLNVSILQGDGSKGLPDAGPFDAIIAAASGPEVPDD
                     LLFQLREGGRLVMPVGEPHEIQQLIKITRTGDEEYEREELGAVRFVPLIGAAGFRDAK
                     GGRHEPRSLPAMIRAAAEPLADLDDPAFGALFDRYAGARVVLLGEASHGTSEYYRARA
                     AISKRLIERHGFTIVAVEADWPDAAAVDRYVRHRPRRDGEEAPFQRFPTWMWRNTDVD
                     AFTGWLRRHNEGREPEAMAGFYGLDLYNLGGSIRAVIDYLDDVDPAAAAVARERYGCL
                     QPYAKNPQGYGRMAITRGFAQCEQPVVQMLHELLRKRMEYAGADGDEYLDAAANARLV
                     ANAEQYYRVMYHGAAESWNLRDTHMFETLCQLLDAKGPDSKAVVWAHNSHIGDASHTE
                     MGQVREELNVGQLCRERFGDAAVLVGFGTHQGTVAAASDWDDPMEVKRVRPSLADSYE
                     RVSHDAGVERFLLDLRGADPAFRAALEEPRLERFIGVIYRPETERWSHYAQASLPGQF
                     DAWVWFDETDAVTPLSGRTEQGGEETYPFGL"
     gene            complement(14953..15132)
                     /locus_tag="VF589_05445"
     CDS             complement(14953..15132)
                     /locus_tag="VF589_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257056.1"
                     /translation="MKKYFSRVVHEGDDEILLIPDEIGFGIGVDVIIERRGDTLTIRR
                     KQTDVATPLAPDAKG"
     gene            complement(15184..15399)
                     /gene="thiS"
                     /locus_tag="VF589_05450"
                     /pseudo
     CDS             complement(15184..15399)
                     /gene="thiS"
                     /locus_tag="VF589_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
     gene            15451..15891
                     /gene="aroQ"
                     /locus_tag="VF589_05455"
     CDS             15451..15891
                     /gene="aroQ"
                     /locus_tag="VF589_05455"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX8257057.1"
                     /translation="MPTPPTIFVLNGPNLNLLGTREPEIYGAATLDEIAGRIEDRARE
                     LGLSVDIRQSNHEGHLIDWLHEAQAAGAKAVILNAGGFTHTSVALHDAIKAVSVPVIE
                     VHLSNPAAREPFRRRSYVAPGARGTIAGFGPLGYELALDAAARL"
     gene            15924..>16077
                     /locus_tag="VF589_05460"
     CDS             15924..>16077
                     /locus_tag="VF589_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HEX8257058.1"
                     /translation="MTDTEGGGDGAMRVDADLVRQLAELLNQNELSEIEVEDGTRRIV
                     VKRQLTA"
BASE COUNT         2640 a         5087 c         5615 g         2735 t
ORIGIN      
        1 ttcatccaaa gaaaacgacc ggaccgccgc taaagcggca gcccggggct gggcccctgc
       61 cttcgcaggg gcacaggtgc ggcacccata tccccttatc ctgaggaggg gactgctaag
      121 gcctcgtccg ggggacagca cgtcccgaca tcaagtccgg catgacgggc ttggcgtcag
      181 tggccgcctt agtggctggc gacgcggccg ccttggcgca gggcggcata tttgagggtg
      241 agatcgggca ggctttgctc gatccaggcg gccatcgcct gctcctcggc cagcgtcgct
      301 tcgagcaaag gcgccgcggc cccgaacccg ccggcctcgg cgagcgcgat cagcgagcgg
      361 taggaggcga tctcgaaatt ctcgaacgcg aaattgacga agctgttctt gaggatctcg
      421 tcggccgcca tgacgttcga aagcgcggcg aaattgccca tgaagttcat cgccatgtcc
      481 ttgaggctcg aatggcgctc gcgaaggccc gacaatattt cgtcgaggcg gccgatctgg
      541 acctcggtct cgcggcggtg gaggcggagc cgctcggcga cttcgggata gcggaccagc
      601 cggtcgagct ggcggtcgat cagggcgagc gcctgattct ccaccgcatg cgcgttctgg
      661 agcccggtca cgaacaatga gcggtaggtg gagcttgggt gggatgccat gatctgcctc
      721 ggtttgcctt gccgctgcaa aagcgcgcgc gggggcctcg gttccctgat ccggcgatcg
      781 gcgcgtgatg agctttcatg tatcccatag cgatggtatc ggtggagcat gaacgtccgg
      841 ccgcccctga cgcttccgga gcgcgcgaag ttcagcgccc acgatttctg gctgctggcg
      901 cggtcgggcg cgttcgacgg ctatgcgaag agcgagctga tcgaggggga aatctgggtg
      961 atgagcgcca tccacagctg gcacgcgaag acgatgtcgc ggctcaatgt ggcgctccac
     1021 tcggcgctgc tcgccgcggg ttcgaatctg gagatattcg tgcccgtgtc gatcgcgctc
     1081 tcggacgatt cggtgcccga gccggatctc gcagtggccg aagatcatga cgacggcccc
     1141 ttgccactcg ccaaggtgaa gctggctatc gaggtgtccg acacaacgct ggagatcgac
     1201 ctcggccgga aagcccttct gtacgcgaag catggcgtac cggaatattg ggtggtggcc
     1261 cggcaaggcg cttgcctcta ccgccattcg tcgccgcaag cgggaggcta cgtcgacaag
     1321 gacaagttcg cgctgggcga gcggatcgag gcggcgtcga ttgaggggct gaccgtagag
     1381 acgggtgaga tcagttagct actgtgcgat tcctcctgcc acttcgcgga tcggttagtc
     1441 actgcccagg ctttcgccaa tgttcatgga tccagatttc gaaaggtggc ggaacctcac
     1501 ctctgtcagc aagatagcgt gcaaatcgat catattctcc gcgcaactgc aactcgcgtt
     1561 tcgacaaaag aacataggca aagagagctg ccgagagcga tgccagcagg atcaggacat
     1621 agtccattca caggttccta aagcatctgg atgcagcgtc cgtcattatt tcattgacgg
     1681 gatgcctcgg cactacggga gctgggagtg tagctttgtc gcccggagaa ggtgccgcgg
     1741 actgtccttg cctagccgaa gcaatggccg ggtggggcgt ctcatttccc caatattggc
     1801 gacctgttcg caacggtcgt cgttccggtc ggaaatggtc ttcaccacca caccacccga
     1861 tgcaacgact agcgccgcag cagccgcaac aagttccttt cgaaaatctc tccagctcga
     1921 cattcgagtc ctctgagaaa gcggacttcc atatatgcac ctgccggcgc taagctgaaa
     1981 gtcttatcga gtcagccgcc ggtacgtcat ccggtgcggc ctgtccgctt ccggccccat
     2041 ccggcgccgc ttgtcctcct cgtaggcttc gaaattgcct tcgaaccatt cgacgtggct
     2101 gtcgccctcg aaggcgagga tgtgggtggc gaggcggtcg aggaagaagc ggtcgtggct
     2161 gatgaccacg gcgcagccgg cgaagttttc cagcgcgtct tccaaggcgc gcaaggtctc
     2221 gacgtcgagg tcgttggtgg gctcgtcgag caggaggacg ttgccgccct gctggagcat
     2281 cttggccatg tggacgcggt tgcgctcgcc gcccgacaat tggccgacct tcttctgctg
     2341 gtcggggccc ttgaagttga acgcgccgac ataggcgcgg gtcgagacct cgtgcttgcc
     2401 gaagcggaac acgtcggagc cgccggagac ctcctcccag acgttcttct tcgcatcgag
     2461 cgcgtcgcgc gactggtcta catagccgag gcgaacggtc tcgccgatgc ggacgccgcc
     2521 ggcgtcgggc tgctcctcgc cgcggatgag cttgaacaag gtggtcttgc cggcgccgtt
     2581 ggggccgatc acgccgacaa tgccgccggg cgggagcgag aaggtgaggt cctcgaacaa
     2641 taatttgtcg ccataggcct tggtgaggcc gttggcttcg atgacggtgc cgcccaggcg
     2701 ctcgggcacc tggatcagga tctgggcctt gccgggggtg cggttctcct gcgcctcgac
     2761 gagctcgtcg aacgccttga tgcgcgcctt ggacttggtc tggcgggctt tcggggaacg
     2821 gcggatccag tccagctcct cctggatcgc cttctggcgg ccttcgtcct cgcgctcttc
     2881 ctgcgccagt cgcttggctt tcttgtccag atagccggaa taattggatt cgtagatgta
     2941 atagcggccg cgatcgagct cgagcaccca gttgacgaca ttgtcgagga agtagcggtc
     3001 gtgggtgacg aggatgacgt tgccggcata gtcgacgaga tgcttttcca gccactggac
     3061 gctctcggcg tcgaggtgat tggtgggctc gtcgagcagc aggatgtcgg gcttttcgag
     3121 cagcagcttg gtgagggcga cccggcgctt ctcgccgccc gagaggttgt ggatcgggct
     3181 gtcgccgggc gggcagcgca gcgcgtccat cgcgatctcg agctggttgt ccagcgacca
     3241 gccatccacc gcatctatct tctcttgcag ctcgcccatc tcggtcatca gcgcgtcgaa
     3301 atcggcgtcc tcgggcggat cgcccatgat cgtcgaaatt tcgttgaagc gctccatcat
     3361 gtcggcgacc ggacggacgc catccatgac gttttccttg accgtcttgg tcggatcgag
     3421 ctgcggctcc tgctcgagat agccaacgcg gatatgctcg ccgggccagg cctcgcccgt
     3481 gaactccttg tcgcggccgg ccatgatctt catcagggtc gacttgcccg atccgttggg
     3541 accgatgatc gcgatcttgg cgtcgggata gaattgcagg ctgatattgt tgaggatggg
     3601 cttgggaacg cccgggaagg tcttggtcat gttcttcatg acgaagctat attgtgcagc
     3661 catggcggcg ccggtctccg aagataatgc tgagcgggat tgggcgctta cctagtggct
     3721 cggcggcgga ttgtcaccct tggagcgagt ggctatcgag ccgaccatga aaaagctttt
     3781 cgccgccacc ggcctcctcg ccgccgcctc gatcgcggcc ctcccctgcc ccgccttcgc
     3841 ccaggccgga tcgaccgacg cgctgcgcga ggcggcgctc ggcgacacgc tcgcctggga
     3901 gattaccgag ggcctcacca ccgaggtcgg gccgcggctg gccgggaccg aggccgaggc
     3961 gcgggcgcgg gactgggcgg tgcgcaggct gaccgggctc ggcttcgcca acgtgcgggt
     4021 cgagacgttc gacatgccgg tgtgggtgcg gggcgaggag cgggccgaga tcatgtcgcc
     4081 gttcccgcag ccgctggcgg tgaccgcgct cggcaatagc ggcgcgaccc gggcgcgcgg
     4141 gctggaggcg gacgtggtcg gcttcgacag cctcgaagcg ctggaggcgg cgagcgatgc
     4201 cagcgtgcgc ggcaagatcg tgttcgtcag ccatgccatg acggcgacgc aggacggatc
     4261 gggctatggc tggttcggcc gcgcccggcg cgaggggccg tcgatcgcca gccgcaaggg
     4321 cgccgcggcg atcgtgatcc gatcgatcgg caccgattat caccgcaacc cgcataccgg
     4381 cgtccagatc tgggccgagg gcgtgaagcc gatcccggcc ggcgcgctgg cgctgcccga
     4441 cgccgagcag ctgcagcgta tattgaagcg gtcggcgggg cggccggtgc ggatgcggct
     4501 gacgctgacg ccgcgcaaca tcggcacgcg ccaatccggc aacgtcatcg ccgagatacc
     4561 gggcagtgat ccggccgcgg gcatcgtgct cgccgcctgc cacctcgaca gctgggacct
     4621 cggcaccggc gcgatcgacg atgcggccgg ctgcggcatc gtcgcctcgg cggctcggcg
     4681 ggcggcggcg ggcgggcggc cgcggcggac gatccgggtg gtgtggttcg gggccgagga
     4741 agtcgggctg ttcggcggcc gcgactatgc ggcgaaacac cgcgacgaga agcatgcggt
     4801 gatcgccgaa agcgatttcg gcgccgaccg ggtgtggcgc ttcaactcct atgtcgggac
     4861 ggcggcgcag ccggtggtgg accggatcgc gcgcgctttg tcgccgctcg gcatcgccaa
     4921 ggggcggacc gacgaggcga gcggatccga catcagcgtg ctttcggcga ccggcgtccc
     4981 gaccttcgag ctcagccagg acggcacgcg gtacttcgac tatcaccaca cgcccgacga
     5041 cacgctcgac aaggtcgacc ccgagcaatt gcggcagaat gtcgcggcct ggacgacgat
     5101 gctggcgatc gccgccaacg cgcccgagga tttcgcggcg atgccgccgc cgggcggaaa
     5161 ttgagctgag ccgggcgggc gcttcgggcg ccgcttattg ggcgaattgc gggaaaggcg
     5221 ggagcttgtc gatatctgcg gcctcgtgct ggagtatcac ggtcgcgtcc aatttgtagg
     5281 cgaggcggcg gaagcgttcg cgcgaagcgg cggtctcggc gcggctggtg ttgaagccgg
     5341 gcattgcgtc ggtgaggacg tcgccatggt tctgccagag gtcgccgctg aggaggacgt
     5401 tgccggaggc gaggcggacc agcagggcgg aatggccagg cgtgtggccg ggggtcatca
     5461 gcattgtgac gcggccgtcg ccgaagacgt ccagatcctc gatgagagcc gcgaccgggg
     5521 cgccgccgga aatccacggg gcgagatggt cggggctgct gcccgcgggc gggggcgtcc
     5581 gcttgagcgc ggtaaggtcg ccggcgccga tcagcagctc ggccttcggg aatttggagg
     5641 cttggccggt atggtcgcca tgataatggc tgatgccgac atattcgatc gcatcgggcg
     5701 cgatgccgat ctcggcgagc tggtcgacga gggtgcggtc gagcgccata ttcgggcgcg
     5761 ggttgccggc gaggcgggcg gacatgccgg cgtcccacag caggtagcgg ccgccgttgc
     5821 gaatcaggta gcagcccacc gggagcaggc gcttgcgcca gggcggcgcg ccggtttcct
     5881 cgcccgaaat gcggccgcaa tcgagccgcc acagggcgat cggggcgggc gcgggcgggg
     5941 cggcgccggc gaggaggagc gcggccgaag cggcggcaat gatgctggcg gtcctggcgg
     6001 gcatgcgccg accttgcggc ggccccggca ggagggggag agggaaagcg gccaacggcg
     6061 cggcggcgcg acgatcgtca cgcgccgcct tgggtcaggc gtcgtagcgg gcggtggtcc
     6121 tggcggcgac ttcctccgcg gtcacgccgg gggcgagctc gatcaggcgg aacgggctct
     6181 ggtgatcggg gcggtggaag acgcacaggt cggtgatcac catatcgacg acgttcttgc
     6241 cggtgagcgg aagcgtgcat tcggggatga acttggggtc gccgttcttc gagacatgct
     6301 ccatcacgac gatgatcttc ttgacgccgg cgacgaggtc catcgcgccc cccatgccct
     6361 tgatcatctt gccggggatc atccagttgg cgatgtcgcc attctcggcc acttccatcg
     6421 cgccgagcac ggtaaggtcg atatggccgc cgcggatcat cgcgaagctg tcggaggagg
     6481 agaaatagga ggacgaaggc agctggctga tggtctgctt gccggcgttg atgaggtccg
     6541 ggtcgacctc gtcctcgtag ggaaagggcc cgatgccgag catgccattc tccgactgga
     6601 gcgtcaccgt catcccctcg ggaatgttgt tggcgaccag cgtcgggatg ccgatgccga
     6661 gattgacgta atacccgtcg cgcagctcct tggcggcgcg ggcggccatc tggttgcggt
     6721 cccaaggcat cattgggctc cagcttgcag ccgccgctgc tgcttttctt cagcggtcag
     6781 aggatcgagc ctagcgattg gcttcctcca gcgagtgaag accacctctt caccggcaag
     6841 tgatgccgca atcagctcgt ccattcggcg cttcgcttcc tcgatcccaa cctgcatgcc
     6901 attcaccctt attctggttc gacgaaaaaa gcggctgttt ttgccacaca gttgcttggt
     6961 agcttcttct gtcctttacg tcgccaacca agccgctgaa tttcctcggc gacgaacccg
     7021 ccagcccatg tcgtggtatc aaaatgcccg tactcgcgaa cggcactctg aatgagcggg
     7081 tcaacgcctg ctaaaggcgc ctcaccgcgc ttcgccgcag atacggctgc ttccaccccg
     7141 gtatcagacc gacagacctt ctcgatttcc tggatcacac gatgctcgcg cggcaagccg
     7201 ccgccggcgg tgtcgagaag gctgttctcg aaaagcagcc tgattatgtt gtccgcatcc
     7261 gctccgtcac cgttgcgagc gtgttcgtag atcaccatcc gctgcttcga agtcatcttc
     7321 tgcagctgtt cgagcgtgat cggcttaccc attcaagccg cctcgcgctg gcggacggtg
     7381 cgaaactcga tcttcttgtc gtagggggcg ccgctgatca ggcgcttcac gtagatgctt
     7441 ggcaggtgga tggagtcggg gtcgaggctg ccgacgggga cgatctcctc gacttcggcg
     7501 acgcagatgc gcccggaggt ggccatcggc tggttgaagt tgcgcgcggt cttgcggaag
     7561 acgaggttgc cggcctcgtc ggccttccag cccttgacga tcgacagatc ggcgcggatg
     7621 ccgcgctcga gtatgtaggt ctcgccgtcg aactccttat gctccttgcc ctcggcgacc
     7681 tcggtgccga cgccggtctt ggtgtagaag ccggggatgc cggcgccgcc cgcgcggcag
     7741 cgttcggcga gcgtgccctg cgggcagaat tcgacctcga gctcgccggc cagatattgg
     7801 cgctcgaact ccttattctc gccgacataa gaggagatca tcttgcggac ctggcgggtg
     7861 cggagcaact tgccgagccc ctcattgtcg atgccggcat tgttagaggc gatggtgaga
     7921 tccttgacgc ccgacgcctg gatggcgtcg atcagccgct cgggaatgcc gcacagaccg
     7981 aacccgccgg cgcagatcgt catgccgtcg aacagcaggc cttcgagcgc cgcttcggca
     8041 ttggcataga ttttctgcat cgctcgctcc cgattggacc tgccgcccga taggcggagc
     8101 gggcaaggcc ggtcaagaaa ggcggcgcag gatcaggcct tgagcttggc cagcactccc
     8161 tggagctgca tggcgttgga catgtcgccc tcgaccttca acttgccctg catgaaggcg
     8221 gtcatgccgt ccagctggcc ggcggcgagc gcctgccaat cttcccagct gaccttgatc
     8281 gtcgtgtcgg cgtcggcgtc ttcctcggtg acttgattgg cctgcccgtc gagcaggatc
     8341 gcgccttggc cggcgccgaa atcgatcttg acgcgcttgc ccggcagcca ggcctgcttc
     8401 tcgttcagcc tggcggccac ttcgctcttg gtcattcgtc gctcccgatt tgagttcggc
     8461 tccccctagt ttacgtgcac gtcaagtcaa gccaggcaga ggggtgaacg ctccgcaccg
     8521 ttcggcctga gcttgtcgaa ggcgcttcct tctcctaccg gcaccggaag caagggcggg
     8581 gcttcgacag gctccgcccg aacgggattt gaatcagcgc cgggatcaca tggacgcagc
     8641 cgaagtcatt cacgagaagt tcgtcgccaa ggtcaaagcc gggaattttc cgcggcggcc
     8701 gaccgacgtg cagccgggcg atgtcggcct cgggcgcgag gcgatggccg agactttcta
     8761 cagccagtgc gtcagccggc agatggaccg gctgtcgcgg cggctgcagg cgcgcggcga
     8821 gggtttctat gcgctcggaa gctcgggcca tgagaagaac gccgcggtgg ccgaggcgct
     8881 gcgcgtcgac gacatggcct ttcttcatta ccggtccaac gccttccaga tccaccggtc
     8941 gaagcagctg ccgggccaga cgccgtgctg ggacatgctg ctgagcttcg ccgcgtcggc
     9001 cgaggacccg atttcgggcg ggcggcacaa ggtgatcgga tcaaagccgc tcaacattcc
     9061 gccgcagacc agcaccatcg cctcgcacct gccgaaagcg gtgggcgcgg cgttcagcat
     9121 cggcatcgcc aagatgctga agctgggcga gacccccctg ccccgcgacg cggtcgtgct
     9181 ggcgagcttc ggcgacgctt cggccaacca ttcgaccgcg caaggggcgt tcaacaccgc
     9241 cgcctggacc gcctatcagg gcagcccgat gccgatcatc ttcctgtgcg aggataacgg
     9301 catcggcatc tcgacgcgca cgccgaaggg gtggatcgag gcctcgttcc gggatcggcc
     9361 ggcgctcaaa tatataaggt gcagcgggct cgatatggcc gacgcctatc gcggcgcggc
     9421 cgaggcggcg cattatgcac gcacccagcg ccggccggtc ttcctccata tggactgcat
     9481 ccggctctac ggccatgccg gatcggacgt acaggcgacc tacctcacca aggcgcagat
     9541 cgaggcggac gaggaacgcg atccgttgct ctacagcgcg gccctgctgg tcgagcagga
     9601 cgtgatgtcg gggcgcgaca tcatcgaaat ctacggcgag gccgaggcga cgctgaaccg
     9661 gatcggcgag caggcgatca cgcggcccaa gctcaccacc agcgccgcgg tgacggcgag
     9721 cctggtgccg cccaagcggc caccggcgcg ggccaacacg cccagctccg aagaccgcaa
     9781 tgccttgttc gcgcgcgacc gcaacatgat ggacaagccg cagcatatgg cgcggctgct
     9841 cagttggacg ctggccgacc tgatgctcgc ccatgacaat atcatcgtcg cgggggagga
     9901 tgtcgggccc aagggcgggg tctacaacgt caccgccaag ctccatgagc gattcggctc
     9961 gtgccgcgtc atcaacactt tgctcgacga gcagagcatc ctcggcctcg gcatcggggc
    10021 ggcgcataac gacatcctcg ccatcaccga gatccagttc ctcgcttacg tccacaatgc
    10081 cgaggaccag atcaggggcg aggcggcgac cctgagcttc ttctcgaacg ggcaatatac
    10141 caacccgacc atcatccgca tcgccggcct cggctatcag aagggcttcg gcggccattt
    10201 ccacaacgac aacagcctcg ccgtcttccg cgacataccg gggctggtgc tcgcctgccc
    10261 gagcaacggg gcggacgcgg tcgggatgct ccgcgaatgc gcccggctcg cgctcgagga
    10321 gcagcgcgtg atcgtgttcg tcgagccgat cgcgctctac atgacccgcg acctccacga
    10381 ggagggcgac gcgctctgga cctcgactta tgccgcgccc ggggaggcga agccggtgcg
    10441 gatcggcgag gtcgcggtcc atggcgaggg gaccgacctc gccatcgtca gctacgccaa
    10501 cggctattac ctgtcgcgcc aggccgagaa gatattgcgc gagcagcacg gcgtgaaggc
    10561 gcgggtgatc gacctgcgct ggctggcgcc ggtcgccgag gagagtttgc tcggcgcggt
    10621 cgcgggatgc gagcggatcc tgatcgtcga cgaatgccgc aagaccgggt cgcagagcga
    10681 ggcgctgatg gcggtgttcg cggagcgggc gccgggcgtg gactgccgcc ggatcaccgc
    10741 cgaggacagc ttcatcccgc tcggccgggc ggcaacgctg acgctgccga gccgcgattc
    10801 tatcgtcgcc gcggcgctgg aggcggtccg tggctgacag gctttccgca ttggtcgtct
    10861 gcccgggccg gggcacttac ggcaaggccg agctcggcta tctcaagcgc ttccatgccg
    10921 acaaggcgga gctgatcgcg agcttcgacc ggctgcgggc cgaggccggg cagccgacca
    10981 tctccgagct tgacggagcg gagcgctaca gcatggcgct gcacacgcgc ggcgacatcg
    11041 cctcgccctt gatcttcgcc gcttcctatg ccgatttcct cgccatcgac cgcgcccgct
    11101 tcgacgtggc agcggtcacc ggcaattcga tgggatggta caccactttg gcctgcggcg
    11161 gcgcggtcga cgcggagcac ggcttcggta tcgtcgatgc gatgggccgg aacagccagg
    11221 cgggcgagcc gggcggacag gtgctgctga ccttggtcga cgagcaatgg cggccggtgc
    11281 cggggcttcg cgagagcttg ctcgggctgg tcgaggcgat cgacggccgg gcggaagcgt
    11341 cgctctatct gtcgatcgac cttggcggca tgctggtgtt cgccggcaac gaggcggggc
    11401 tggccgccct gctcgccgaa gcgccgccga cgccggggcg cgagccgctg cggctggtca
    11461 atcacggacc gttccacacg ccgctgatgc agggctcgtc ggaccgcgcc ttcgaactgc
    11521 cggcggaatg ggtcaaaggg cccgcgatcc cgatgatcga cgggcgcggg catatctggc
    11581 gcccgttcgc gagcaaccca caggcgctcc gccattatac gttcgcgacc cagatcttgg
    11641 agagctacga cttcacccgc gccgtccagg tcgccgtgcg cgagcatgcg ccggaccgga
    11701 tcatcctgct cggcccgggc gacacgctcg gcggggcgat cgcccaggcg ctgatcgcga
    11761 tcgaatggcg cggaatgcgc tccaagcagg acttccagga gatgcagacg tccgcctcgc
    11821 cgatcctgct ttcgatgggc cgcgaggagc agcgcgcgct ggtcgcggcc tgacccgcga
    11881 tccgggatca agccgcggtt aagctgccgc agatcaagag cggtgcccaa tcggggccgg
    11941 tgcgctatag gcgttctcga aaatcaaaga ggctgttcat gctgaagacg atgatgagag
    12001 tgttgccggt gccgttgctg ctggcggcgg gcgcggcggc ggcgcaggtg gatatgaccg
    12061 acgacgccgc gatgcggccc aagctggagg cgctggcggg ggccggcaat gccgaggcgg
    12121 cctatcatct cggcatgatg caccatctcg gcgccaacgg ggccaagaag gacccgcaaa
    12181 gggcgctcga gctgtttcgc caggcggcgg agggcggcga cccgctcggc gcctacaagc
    12241 tcggcagcta ttatgacggc cagggcggcg gccttgtcca gcccgacccg gagctcgcgc
    12301 tcaagcacaa gatggtggcg gccaaggccg gctattcgct ggcccagcac gacgtcgcca
    12361 aattgctcta cgcgcgcggc gacaccgggg aggcggtgcg ctggctcgag aaatcggcgc
    12421 gccaaggcta tggggaatcg ctgcaggcgc tggcctcgct ctataccggc gagggcaagg
    12481 tcgcgaagaa tatgggcaag tcgctcgcct atctcgtcat tctgcagaac agcctcaagg
    12541 gaaagaaggc gtcgccgaag atccaggcgt gggtcaagga aacgcgggcc aagctcagcc
    12601 cggccgagat cgcgcaggcg gaggatgtgg tgcgcggctg gaaggtgaag ccgagcccgc
    12661 tgacgctgca ggcgctggcg ggcgaggaga cggccaagac attggtggcg aagacgccgg
    12721 cgccggctgc ggcggacgca gagccggctg cggcggaggc cgctccggcc gaggcggatg
    12781 ccgctccggc cgaggcaggt ccggcgccgc aggcggagcc ggcaccggcg gggccggacc
    12841 gggcataatc gttccccggc gaaggccggg gtccagctgc gctggagtct ggggcccggc
    12901 cttcgccggg gaacaagttc gcgtgaacat cagaggccga aggggtaggt ttcctcgccg
    12961 ccctgttcgg tgcggccgga gagcggcgtc accgcatcgg tctcgtcgaa ccagacccag
    13021 gcgtcgaact ggccgggcag cgaggcctgg gcataatggc tccagcgctc ggtctcaggg
    13081 cggtagatga cgccgatgaa gcgttcgagg cggggctcct cgagcgcggc acggaacgcg
    13141 gggtcggcgc cgcgcaggtc gagcaggaag cgctcgacgc cggcatcgtg cgagacccgt
    13201 tcgtagctgt cggcgagcga ggggcggacg cgcttcacct ccatcggatc gtcccagtcg
    13261 ctggcggcgg cgacggtgcc ctggtgggtg ccgaagccga ccagcaccgc cgcgtcgccg
    13321 aaccgttcgc ggcagagttg gccgacgttc agctcctcgc gcacctggcc catctcggta
    13381 tggctggcat cgccgatatg gctgttatgc gcccacacca cggctttaga gtccggcccc
    13441 ttggcgtcca gcaattggca gagcgtctcg aacatgtggg tgtcgcgcag attccagctc
    13501 tccgcggccc cgtgatacat cacccgataa tattgctcgg cattggcgac gagccgggca
    13561 ttggcggcgg cgtcgagata ttcgtcgccg tccgcgccgg catattccat gcgcttgcgg
    13621 agcaattcgt ggagcatctg gacgaccggc tgctcgcatt gggcgaagcc gcgggtgatc
    13681 gccatccggc catagccttg cgggttcttg gcataaggct ggaggcagcc gtagcgctcg
    13741 cgggcgacgg cggcggcggc gggatcgaca tcgtcgagat agtcgatgac ggcgcggatc
    13801 gagccgccga gattgtagag gtcgaggcca tagaagccgg ccatcgcctc gggctcgcgc
    13861 ccttcattgt ggcggcgcag ccagccggtg aaggcgtcga cgtcggtgtt gcgccacatc
    13921 caggtcggga agcgctggaa cggcgcctcc tcgccgtcgc ggcgcgggcg gtggcggacg
    13981 tagcggtcga ccgcggcggc atcgggccag tcggcctcga ccgcgacgat agtgaagccg
    14041 tgacgctcga tcaggcgctt cgaaatggcg gcgcgggcgc ggtaatattc ggacgtgccg
    14101 tggctggcct cgccgagcag gaccacgcgg gcaccggcat agcggtcgaa cagcgcgccg
    14161 aaggcggggt cgtcgaggtc ggcaagcggc tcggcggcgg cgcggatcat cgcgggcagc
    14221 gagcgcggct cgtggcggcc gcccttggcg tcgcggaagc cggcggcgcc gatcaagggg
    14281 acgaagcgga cggcgccgag ctcttcgcgc tcatactcct cgtcgcccgt gcgggtgatc
    14341 ttgatcagct gctggatttc gtgcggctcg ccgaccggca tgacgaggcg gccgccctcg
    14401 cgcagctgga acagcaaatc gtcgggaacc tccgggccgc tcgcggcggc gatgatggcg
    14461 tcgaacgggc cggcatcggg caggcccttg ctgccgtcgc cctggaggat ggagacgttg
    14521 aggtaaccga accgctccat gcgttctgtc gcgagccgga ccagctcggc atggcgttcg
    14581 atcgcgatca cctcgccggc gacctggccc atgatggcgg cggcatagcc cgagccgccg
    14641 cctatctcga gtacgcggtc gccgccgccg agctcggccg cctcgatcat caaggcgacg
    14701 atatagggct gcgagatggt ctgctcggcc tcgatcggca gcggcgcatc gtcgaaggcg
    14761 aattccttga gatgcccggg cacgaactcc tcgcgcggca ccaggcgcat ggcgtcgagg
    14821 aggcggggat cgctgatccc gcgggcggcg atctgccgct ccaccatctt ctcgcgcgcg
    14881 gcggcgaaat cggtcatggc gggcctcctc ccgcgagaaa accagcgcct catgcgcgcg
    14941 ttccgagaag gatcagcctt ttgcgtcggg tgcgagaggt gtggcgacgt ccgtctgctt
    15001 ccgccggatc gtcaaggtgt cgccgcggcg ttcgatgatc acgtcaacac cgatgccgaa
    15061 cccgatttcg tcggggatca gcagaatctc atcgtcaccc tcgtggacga cacggctgaa
    15121 gtatttcttc atgagcacat catagcagaa gtcgccgctc acgtctcggt gagcgccgct
    15181 tcagccgcct ccgacgaagg tgacgatttc gtaatcgtcg ccgtcctcga tcttcacgtc
    15241 ggccagagtc gagcggggga cgatttcgag gttgcgctca acggcgaccc tggtcggttc
    15301 caggccgagc tcgagcgcga gatcggcgat ggtcatgccc ttgaggacgc ggcggtgctg
    15361 gccgttgacg gtgatcgaaa gcgtgtcgtc atagaccatg gaaaagctcg cctttgcgca
    15421 acgggttcgg ccgccatata ggaagcggcc atgccgacgc caccgaccat cttcgtgctc
    15481 aacggcccga acctcaacct gctcggcacg cgcgagcccg aaatctacgg cgcggcgacg
    15541 ctcgacgaga tcgccgggcg gatcgaggat cgggcgcggg agctcgggct gtcggtggac
    15601 atccgccagt ccaaccatga gggccacctg atcgactggc tgcacgaggc gcaggcggcc
    15661 ggggccaagg cggtgatcct caatgccggc ggcttcaccc acacctcggt cgcgcttcac
    15721 gacgcgataa aggcggtgtc ggtgccggtg atcgaggtcc atctgtcgaa cccggcggcg
    15781 cgggagccgt tcaggcggcg gagctatgtg gcgccgggcg cgcgaggcac gatcgcgggc
    15841 ttcgggccgc tggggtatga gcttgctctg gacgcggcgg cgcgtctctg acagtgagcc
    15901 ccgaacaggg aaaagagggt ccgatgaccg acaccgaagg tggcggcgac ggcgcgatgc
    15961 gggtcgatgc cgacctcgtc cgccagctcg ccgagctgct gaaccagaat gaactcagtg
    16021 aaatcgaagt tgaggacggc acccggcgga tcgtcgtcaa gcgccagctg acagccg
//
LOCUS       DASVRK010000140         6076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_212358, whole genome shotgun sequence.
ACCESSION   DASVRK010000140 DASVRK010000000
VERSION     DASVRK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212358"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VF589_05465"
     CDS             complement(<1..274)
                     /locus_tag="VF589_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8257059.1"
                     /translation="MTAALPAPCDDGLALTTEARDGSHRRWTLVASILASSLAFVDGS
                     VVNVALPAIGADLGAGAAGLQWIVNAYLLPLSALLLLGGGAGDKFGR"
     gene            390..884
                     /gene="msrB"
                     /locus_tag="VF589_05470"
     CDS             390..884
                     /gene="msrB"
                     /locus_tag="VF589_05470"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8257060.1"
                     /translation="MTDAPALDQAVFDLTPPSDDQLRALAAELDDQERRVLLEHGTEA
                     PFCGIFLDEKRDGIFTCRLCGLPLFGGGTKFESGTGWPSFTTPFAEGHLETIRDTSYG
                     MIRTEIVCARCGGHQGHVFPDGPPPTGERYCINSVSLEFTPAGEPLPDKLGRGAPEGE
                     AWTG"
     gene            916..1674
                     /locus_tag="VF589_05475"
     CDS             916..1674
                     /locus_tag="VF589_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEX8257061.1"
                     /translation="MSGWLAAGVYGLLSASGLIAGALAGLGLQMRHRLIAAVTAVGVG
                     LLIAAASLYLISGALEAVSAATAASGALAGAAAFSLANLALRRWQAQSRKRCGECAPQ
                     PREADLPGSGAAIAIGSLLDAVPEALIIGASAAGGGEALPPAALIAAFALGNFAEGLS
                     SATGMRDAGRSRRYVLLLWTGATLLTGAAAVLGFALFSGGGGAQRGWLEAFAAGALLA
                     LVVETMAPEAVAGHPGFTGLLAMLGFSALLLALA"
     gene            1805..2740
                     /gene="lepB"
                     /locus_tag="VF589_05480"
     CDS             1805..2740
                     /gene="lepB"
                     /locus_tag="VF589_05480"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8257062.1"
                     /translation="MNEDREAPPRRSFGARAGIVLLNLLAPGAGLIRTGHWRAGLGFA
                     AGMSVWLLALTAAAAMLPMVTPTGFIVVIVLSLVFVIILLIVPTVASWRASRFRPAAV
                     PPWGRWYALLSLFILCQLASIQAVRGLHGFYRPFYAPAEAMHPTILTGERFVADMRGD
                     RAPRIGEIILLNVGDAIYVKRVAALDGDRIAMRDGVPIVNGVPAAQREVGTIIVYEDS
                     EPSPSKIIAERLPGQSGEHRILDMGVTDFEDQAEVTVPPGHLFVLGDNRDRSADSRVP
                     RDMHGVEMLPMGDVVGRPLFKTWTADWQWLGTPLD"
     gene            2836..3870
                     /locus_tag="VF589_05485"
     CDS             2836..3870
                     /locus_tag="VF589_05485"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8257063.1"
                     /translation="MGGTPIGVRLLALLAILLLLVALPAGALLWMTSVPGRSHSGPLP
                     PLSSAEAGLAANLRRHVSAVASEPHNVGYPEALEASARYIEQTLAGLGYQVRRQPFAA
                     AGQQVRNLEVVIEPAAPAAETLVVGAHFDSCCDAPGANDNGTGTAAVIELARSLADLS
                     GKSSLRIRLVLFVNEEPPFFKTDLMGSLVYAKRLKQTGEPVLGMISLETLGYYSDAER
                     SQHYPPPLGLLYPHKGDFVAFVGLTSSRAFVRKAVASFRAHAAFPSVGGTAPGIVPGI
                     DWSDHWSFEQVGIPALMLTDTAPFRYPHYHSIADTPDKVDYERLARVVAGLDRTIRGW
                     ALPPTLGMAG"
     gene            complement(3982..4554)
                     /locus_tag="VF589_05490"
     CDS             complement(3982..4554)
                     /locus_tag="VF589_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257064.1"
                     /translation="MKRIGLVAGAALLAFAIPGVAHAQVGAVTGAHPFVGVQVGHHDL
                     GLDLDEVDTGSFDVDDSGLIYGVYGGVDFDAGSSLVVGVEGNFNLGSGPIDSDFGAAG
                     RVGLRAGLGAVIFARAGYQWVNVSAEGLLDLDEGVIDDDELDIDDTVGNYLVGVGADF
                     AVGSNMGIRAAVDTIAFDSIRPTVGVHFRF"
     gene            complement(4857..5426)
                     /gene="pth"
                     /locus_tag="VF589_05495"
     CDS             complement(4857..5426)
                     /gene="pth"
                     /locus_tag="VF589_05495"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX8257065.1"
                     /translation="MQIWVGLGNPGAQYAMHRHNVGFMAIDAIAEVHRFDPPKKTFQG
                     WAQQGRIGGARILLLKPATFMNDSGRSVRSAMDFFKKEAGDVTLFHDELDLAPFKVKV
                     KLGGGTAGHNGLRSTEAHIGNAFRRVRIGIGHPGHKDRVTGHVLGSYAKAETDDLADM
                     LGAIAAEAEWLARGDDARFMNEVALRLQG"
     gene            complement(5536..>6076)
                     /locus_tag="VF589_05500"
     CDS             complement(5536..>6076)
                     /locus_tag="VF589_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX8257066.1"
                     /translation="EKALNKMLSGGHFMNSVVMIDVAGGQARTLPKDVQFHPVTDRPL
                     HVDFLRISEHSKVTVAIPIRFVDEEESRGLRRGGVLNTVRHELELVCDAAEIPDEVTI
                     SLAGLDIGDSLHISAVTLPGGSESAITDRDFTIATIVAPSGLKAETDDDATPPADEVP
                     ATEATRGDEPEDDPAQTQE"
BASE COUNT          881 a         2153 c         2040 g         1002 t
ORIGIN      
        1 gccgcccgaa cttgtcgccg gccccgccgc cgagcagcag caaggcgctc agcggcagca
       61 gataggcgtt gacgatccat tgcagcccgg ccgcgcccgc gccgagatcg gcgccgatcg
      121 ccggcagcgc gacgttcacc accgacccgt cgacgaaggc gaggctcgac gccaggatcg
      181 aggcgaccag ggtccagcgc cggtgcgagc cgtcccgcgc ctccgtcgtc agcgcgagcc
      241 cgtcgtcgca cggcgcgggg agggcggcgg tcatgcgcca ccatcccggc cttcgccgcc
      301 cgcgtcaatg caccgccgcc ggcccgtcct cccggttgcc gcccgccgcg gattggagga
      361 tagaggcggc gctgaacggg agaatgccca tgaccgacgc ccccgccctc gaccaagccg
      421 tcttcgatct cacgccgccc agcgacgacc agcttcgcgc gctcgccgcc gagctcgacg
      481 accaggagcg ccgcgtgctg ctcgaacacg gcaccgaggc gccgttttgc ggcatcttcc
      541 tcgacgagaa acgcgacggc atcttcacct gccggctgtg cggcctgccc ttgttcggcg
      601 gcggcaccaa gttcgaaagc ggcaccggct ggccgagctt caccaccccc ttcgcggaag
      661 ggcatctcga gaccatccgc gacaccagct acggcatgat ccgcaccgaa atcgtctgcg
      721 cccgctgcgg cggccaccag ggccacgtct tcccggatgg cccgccgccg accggcgagc
      781 gctattgcat caactcggtc agcctcgaat tcacgcccgc cggcgagccg ctccccgaca
      841 agctcggccg cggcgcgccg gaaggcgagg cgtggaccgg ctgaagcccc gccttggccg
      901 tccggcccgc cgcgagtgag cggttggctg gccgcgggcg tttacggcct cctgtcggcc
      961 tccgggctga tcgccggcgc gctggccggc ctggggctgc agatgcggca ccggctgatc
     1021 gcggcggtga ccgcggtcgg cgtcggcctg ctgatcgcgg ccgcctcgct ctacctcatc
     1081 tccggcgcgc tcgaggccgt ctccgccgcc accgcggcgt cgggcgcgct ggccggagcg
     1141 gccgcgttca gcctcgccaa tttggcgctg cgacgctggc aggcacagag ccgcaaacgc
     1201 tgcggcgaat gcgcccccca gccgcgcgag gccgacctgc ccggcagcgg cgccgccatc
     1261 gccatcggct ctttgctcga tgccgtgccc gaagccctga tcatcggcgc ttccgcggcc
     1321 ggcggcggcg aggcgctgcc gccggccgct ttgatcgccg ccttcgcgct cggcaatttc
     1381 gccgagggcc tgtcgagcgc gaccggcatg cgcgacgccg gccgcagccg ccgctacgtc
     1441 ctgctgctgt ggaccggcgc caccttgctg accggcgcgg cggcggtcct cggctttgct
     1501 ctgttcagcg gcggcggcgg cgcccagcgc ggctggctcg aggcgttcgc ggccggagcg
     1561 ctgctggcgt tggtggtcga aaccatggcg ccggaggcgg tcgcgggcca tcccggcttc
     1621 accggcctcc tcgccatgct cggcttctcc gccctcctcc tcgccctcgc ctgaccgtcc
     1681 tccggcgaaa gccggggtcc agcggcgcct ccgaaacgcc cggagccctg agcacaaccc
     1741 gcttccagtc caggtcccca gcctctaccc tccgcagcgg cgcagcgcct gaagatgggg
     1801 gaatatgaac gaagatcggg aggccccgcc gcggcggtcg ttcggagcgc gcgccggcat
     1861 cgtcctgctc aatctgctcg caccgggcgc cggcctgatc cggaccggcc attggcgcgc
     1921 ggggctcggc ttcgcggcgg ggatgtcggt gtggctgctc gcgctgacag ccgccgccgc
     1981 catgctgccg atggtgactc cgacggggtt catcgtcgtg atcgtcctgt cgcttgtctt
     2041 cgtcatcatc ctcttaatcg tgccgacggt cgcgagctgg cgggcgagca ggttcaggcc
     2101 ggcggcagtg cctccatggg ggcgctggta cgccctgctg agcctcttca tcctgtgtca
     2161 gctcgccagc atccaggccg tgcgcgggct tcacggcttc tatcgtccgt tctacgcgcc
     2221 cgccgaggcg atgcatccga ccatcctcac gggcgagcgg ttcgtggccg acatgcgagg
     2281 ggaccgtgcg ccgcggattg gggagatcat tctcctgaac gtcggcgacg ccatctatgt
     2341 gaagcgcgtg gctgcgctgg acggggaccg gatcgccatg cgggacggcg tgccgatcgt
     2401 gaacggcgtt cccgcggccc agcgggaggt cgggaccatt atcgtgtacg aagattcgga
     2461 gccttccccc tcgaaaataa tcgcggagcg gctgccggga caatccggcg agcatcgcat
     2521 cctcgatatg ggagtcaccg atttcgagga ccaggcggag gtgaccgtcc caccgggcca
     2581 tctgttcgtg ctgggcgaca atcgcgatcg gtccgccgac agccgcgtcc cgcgcgatat
     2641 gcacggagtc gagatgttgc ctatgggcga tgtcgtcggt cggccgctgt tcaagacctg
     2701 gaccgccgac tggcagtggc tcggcacccc gctcgattga atatcgacct cggccggcac
     2761 tgccttggcc tcagcgcgtt aaggacggtc gttcgcttct gctaaccctt cgccatcttc
     2821 cctaacggag cgcgcatggg cgggaccccg atcggagtaa ggctgctggc cctccttgcc
     2881 attttgctac tgctggtcgc gcttccggcg ggcgccttgc tctggatgac ctcggtgccg
     2941 ggccgctccc attcggggcc gctgccgccg ctttcgtccg cggaagccgg gctcgccgcg
     3001 aacctccgcc ggcacgtttc ggcggtggcg agcgagccgc acaatgtcgg ctaccccgag
     3061 gcgctggagg cgtcggcgcg ctatatcgag cagacgctgg ccggcctcgg ctatcaggtc
     3121 cgccggcagc cgttcgcggc cgccgggcag caggtccgca atttggaggt cgtgatcgag
     3181 cccgccgcgc ccgccgccga gacgctcgtc gtcggcgccc attttgacag ctgctgcgac
     3241 gcgcccggcg ccaacgacaa tggcaccggc accgccgccg tgatcgagct tgcccgctcg
     3301 ctggccgacc tgagcggcaa gtcgtcgctg cgcatccggc tggtgctgtt cgtcaacgag
     3361 gagccgcctt tcttcaagac cgacctgatg ggcagcctcg tctatgcgaa gcgcctgaag
     3421 cagaccggcg agccggtcct cggcatgatc tcgctggaga cgctcggcta ttatagcgac
     3481 gctgagcgca gccagcatta tccgccgccg ctcggcctcc tctatccgca caagggcgat
     3541 ttcgtcgcct tcgtcggcct gacctcgtcc cgcgccttcg tgcgcaaagc ggtcgcctcg
     3601 ttccgcgccc acgccgcctt tccgagcgtc ggcggcaccg cgcccggcat cgtgccgggc
     3661 atcgactggt ccgatcattg gtcgttcgag caggtcggga tcccggcgct gatgctcacc
     3721 gacaccgcgc cgttccgcta cccgcattat cacagcatcg ccgacacgcc cgacaaggtc
     3781 gattacgagc ggctcgcgcg ggtggtggcg gggctggacc ggacgatcag gggctgggcg
     3841 ttgccgccga cattgggcat ggccggctag gcccggccgc cggtcggccg agcctcaggc
     3901 gtgcgcggcg ccttcggccc gcgaaataaa gtggccggtc atacggaccg gccactcctt
     3961 tgaagcgctc ggctcttcag gtcagaaccg gaagtgcaca cccacggtgg ggcggatgct
     4021 gtcgaaggcg atcgtatcca ccgccgcacg gatgcccatg ttgctgccga ccgcgaaatc
     4081 cgcgccgacg ccgacgagat agttgccgac ggtgtcgtcg atgtcgagct cgtcgtcgtc
     4141 gatcacgcct tcatcgaggt cgagcagccc ttcggcgctc acgttgaccc actgatagcc
     4201 ggcccgggcg aatatgaccg ctcccaagcc cgcacgcagg ccgacgcggc cggcggcgcc
     4261 gaagtcggaa tcgatcggcc cgctgccgag gttgaaattg ccctcgacgc cgaccaccag
     4321 cgaggagccc gcgtcgaaat cgacgccgcc atagacaccg tagatgaggc cggaatcatc
     4381 gacgtcgaag ctgcccgtgt ccacctcatc gaggtccagg ccgaggtcgt gatggccgac
     4441 ctgcacgccg acgaagggat gagcccccgt caccgccccg acctgcgcat gcgctacgcc
     4501 cggaatggca aaggctaaca gcgccgcgcc ggcgactaac ccgattctct tcatgattga
     4561 cctttcccca cttgtctacc gcttgattgc gatcggcagc ggcggctact tgggggaata
     4621 actgttattg atcaagcacc tgacctacaa tttgaactgg tgtgctaggg aagcaacaag
     4681 ctggatcaac ttggggtggg gcagggccag gcggcccggt cgctaccttg tcggggtccg
     4741 aaaggggaag tgagattcca gcttgcgctg gaatgacggg tggagagtgc gggaaactaa
     4801 ggtcctcccc tgcaaatgca ggggaggatt tctgtgggcg tcattccagc gagagctcag
     4861 ccctgcaatc tcagcgccac ctcgttcatg aaccgggcat cgtcgcccct ggccagccac
     4921 tcggcctcgg cggcgatcgc gccgagcata tccgccagat cgtcggtctc ggctttcgcg
     4981 taactgccga gcacgtggcc ggtcacccgg tccttgtggc cgggatggcc gatgccgatc
     5041 cggacgcggc gaaaggcgtt gccgatatgg gcttcggtcg agcggaggcc gttatggccg
     5101 gcggtgccgc cgccaagctt caccttgacc ttgaacggcg cgagatcgag ctcgtcgtgg
     5161 aacagggtga cgtcgccagc ctccttcttg aagaagtcca tggcggagcg gaccgatcgg
     5221 ccgctgtcgt tcatgaaggt ggccggcttc agcagcaata tgcgcgcgcc gccgatccgg
     5281 ccttgctgcg cccagccctg gaacgtcttc ttgggcggat cgaaacggtg cacctcggcg
     5341 atggcgtcga tcgccatgaa gccgacattg tgccggtgca tcgcatattg cgcgccggga
     5401 ttgccgaggc cgacccagat ctgcacgggc tcgaactccc catccgccgc cgcctccgcc
     5461 aaatccggct ggcccggcac ctccaccttt ttgggtgcga agagggcgcc gagccggccg
     5521 agcaaggaca aaggcttatt cctgcgtctg ggcgggatcg tcctcgggct cgtcgccgcg
     5581 ggtggcctcg gtcgccggga cctcgtcggc cggcggtgtc gcgtcgtcgt cggtctcggc
     5641 cttcaggccc gaaggcgcga cgatggtggc gatggtgaag tcgcggtcgg tgatcgccga
     5701 ttcggagccc cccggcagcg tcaccgctga aatgtggagc gaatcgccga tgtcgaggcc
     5761 cgccaggctg atcgtcactt cgtccggaat ctcggcggcg tcgcagacca gctcgagctc
     5821 gtgacggacg gtgttgagca cgccgccgcg gcgcaggccg cgcgattcct cctcgtccac
     5881 gaaccggatc gggatcgcga cggtaacctt gctgtgctcg gagatgcgca ggaaatcgac
     5941 atgcagcggc cggtcggtga ccgggtggaa ctggacgtcc ttgggcaggg tccgcgcctg
     6001 gccgccggcg acgtcgatca tcacgaccga gttcatgaag tggccgccgg acagcatctt
     6061 gttcagcgcc ttttcc
//
LOCUS       DASVRK010000141         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_212891, whole genome shotgun sequence.
ACCESSION   DASVRK010000141 DASVRK010000000
VERSION     DASVRK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212891"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1169
                     /locus_tag="VF589_05505"
     CDS             93..1169
                     /locus_tag="VF589_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8257067.1"
                     /translation="MSAGFHSLEIAEIVSETAEAKSIRFDLPEALRQTFSFKPGQHLT
                     LKAEIEGEDVRRNYSLCVAPQEGELKVTVKRIAGGVFSNWANDHLKPGDAIEVMPPHG
                     SFTWDFRPGANRHYVGFAGGSGITPLMSLMKTALTTEPDSRFTLLYGNRDSQSVIFLE
                     KLGQLKNRFMSRLQVHHFLAEEAEDIELFNGLLDRDKCDDILARLLDPKAVDAFFICG
                     PGPMMDAAEAAIMAHGVPHERILIERFTAGRPSAAIAAQMQALQQEAQGLGMLVTLDG
                     RKRRVPFDAAAGNILDSARTAGLPAPFACKAGVCATCRARVVSGEVEMAARYGLSDDE
                     VAAGYVLTCQSVPKGEGVEIDYDA"
     gene            complement(1157..1753)
                     /locus_tag="VF589_05510"
     CDS             complement(1157..1753)
                     /locus_tag="VF589_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8257068.1"
                     /translation="MARPQSPDYDKRRDGIVAEAARLYARRGFNGASIADLAQACSTS
                     KSLIYHYFPGKEDILYEVMAAHLDALVEAADAAVRAGSPEERLRALAIAFMQLYVGAQ
                     DSHKVLLNELDNLPSGRREEVVAKQRRIIAVVEALMGEIRPDARPLALPLAMLFFGMI
                     NWTHTWFRADGKIGPKALAEMSVNLMLQGLIRAELQAS"
     gene            complement(1833..3035)
                     /gene="bla"
                     /locus_tag="VF589_05515"
     CDS             complement(1833..3035)
                     /gene="bla"
                     /locus_tag="VF589_05515"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX8257069.1"
                     /translation="MKVSRTPAGRRKLSRATKAALVGVIGLSGTLAATLPTSKAAQSY
                     PPPPRAAPRASQPFTRQYLPATPPAVLSAQRSLDSRVALIGRGFNGDVGIAVKDIQTG
                     WVSHYDGARHFPQQSVSKFWVALTALDKIDRGELDIYSPVTVRHQDMTVFHQPIRAMI
                     KGGGFNTNVSDLLHRALQQSDNTANDVVMRRAGGPEAVRDFLARKGIDGIRFGPGEKL
                     LQAGIAGLTWRPEYSVGRAFYTARAGVPASVRQAAYNRYVSDPIDGATPLGIVDALAR
                     LKRGELLSASSTQRLIAIMSNTKTGPQRLKAGLAPGYRLAHKTGTGQVLGATQSGYND
                     IGIVTAPNGRSYAVAVMIRRTAAPVPQRMQMMQSVTRAVVDYDRDMDGQDWAGASGHR
                     AGAGSSSK"
     gene            complement(3164..3517)
                     /locus_tag="VF589_05520"
     CDS             complement(3164..3517)
                     /locus_tag="VF589_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257070.1"
                     /translation="MIDRVITDSFADQRGDDSRGAVQRRVATTVALALARVQRDPALS
                     PTLAPALDQRLRRLAAMLQKREGNEAEQDWARGLARLLADREALDKVLADPKRAPQIP
                     PGMPIGSAEDEWLAE"
     gene            complement(3554..>4203)
                     /locus_tag="VF589_05525"
     CDS             complement(3554..>4203)
                     /locus_tag="VF589_05525"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX8257071.1"
                     /translation="WDMFAVDWLYGTDSEAEALTKAAAAVAEGLRFVADNDARPTGSG
                     HPQGAIWDDFGDPATELERMTAVRRAAVERFGVGALAGVEPVANLRRKFVPIWLLHRY
                     QVDAAAKLVGGVDFSYSLRGDGREAATLVAEAAQRRALEALLATLRPAELEVPAGLLP
                     YLSAGWSGNPDRQYDIEIFRTAGGPVFDPLAASEAAATVTLNALLAPERLNRLQI"
BASE COUNT          626 a         1428 c         1460 g          689 t
ORIGIN      
        1 ctgcaaggcg caatggcgct gccgcgactg ccttgagccg ttcgacctgt tcaaatgcca
       61 ttgattgaac gacagccccg ggaagcgccg cgatgagcgc cggtttccat agcctcgaga
      121 tcgccgagat cgtgtccgaa acggcggagg cgaagtcgat ccgcttcgat ctgcccgagg
      181 cgctgcgcca gaccttttcc ttcaagcccg gccagcattt gacgctcaag gctgagatcg
      241 aaggtgaaga cgtccggcgc aattactcgc tctgcgtcgc gccgcaggag ggcgagctca
      301 aggtcaccgt caagcgcatc gccggcggcg tcttttccaa ctgggccaac gaccatctga
      361 agccgggcga cgcgatcgag gtgatgccgc cgcacggcag cttcacctgg gacttcaggc
      421 cgggtgcgaa ccgccattat gtcggcttcg ccggcggctc cgggatcacg ccgctcatgt
      481 cgctgatgaa gacggcgctg acgaccgagc cggacagccg cttcaccttg ctctacggca
      541 atcgcgacag ccagtcggtg atcttcctcg agaagttggg gcagctcaag aatcgtttca
      601 tgagccgcct ccaagtccat catttccttg ccgaagaagc cgaggacatc gagctgttca
      661 acgggctgct cgatcgcgac aaatgcgacg acatcctcgc ccgtctgctc gatcccaagg
      721 cggtcgacgc cttcttcatc tgcggccccg ggccgatgat ggatgccgcc gaggccgcga
      781 tcatggcgca cggcgtgccg cacgagcgca tcctgatcga gcgtttcacc gccggccgcc
      841 cctcggccgc gatcgccgcc cagatgcagg cgctccagca ggaggcgcag gggctgggaa
      901 tgctggtcac gctcgacggc cgcaagcggc gggtgccgtt cgacgccgcc gcgggcaata
      961 tcctcgacag cgcccgcacc gccggcctgc cggcgccgtt cgcctgcaag gccggcgtct
     1021 gcgccacctg ccgcgcccgc gtcgtctccg gcgaggtcga gatggctgcg cgctacgggc
     1081 tcagcgacga cgaggtcgcc gccggctacg tcctcacctg ccagtcggtg ccgaaaggcg
     1141 agggcgtcga gatcgactac gacgcctgaa gctcggccct tattagccct tggagcatca
     1201 gattaactga catctcggca agtgctttcg ggccgatctt gccgtcagct ctgaaccagg
     1261 tgtgggtcca gttgatcatc ccgaagaaca gcatcgccag cggcagcgcg agcggccgcg
     1321 cgtccggcct gatctcgccc atcagggctt cgaccacggc gatgatccgc cgctgcttgg
     1381 cgaccacttc ctcgcgccgt cccgacggca ggttgtcgag ctcgttcagc agcaccttgt
     1441 ggctgtcctg cgcgccgaca tagagttgca tgaaggcgat ggcgagggcg cgcagccgct
     1501 cctccgggct gccggcgcgg acggcagcgt cggccgcctc cacgagcgcg tcgagatgcg
     1561 ccgccatgac ttcgtagagg atatcctcct tgcccgggaa ataatggtag atcaaagatt
     1621 tcgacgtgct gcacgcctgg gcgaggtccg cgatcgacgc gccgttgaag ccgcgccggg
     1681 cgtagagccg cgccgcctcc gcgacgatgc cgtcgcggcg cttgtcgtaa tcgggggatt
     1741 ggggccgggc catcgccgcc gcaccttaaa gcgctccgcg ccaaggtcca cttccctcat
     1801 cacccccagt tcggggtgag cccccgcccg gcttacttgc tcgaggatcc cgctccggcg
     1861 cggtggccgc tcgcgcccgc ccaatcctgg ccgtccatgt cgcggtcgta atcgaccacc
     1921 gcgcgggtga cgctctgcat catctgcatg cgctgcggga ccggcgcggc cgtgcggcgg
     1981 atcatcaccg cgaccgcata ggagcggccg ttgggcgcgg tgacgatgcc gatgtcatta
     2041 tagccgctct gggtggcgcc cagcacctgg ccggtgccgg tcttgtgcgc cagccggtaa
     2101 ccgggcgcga gtccggcctt gaggcgctgc ggcccggtct tggtgttgga catgatcgcg
     2161 atcagccgct gggtcgagct ggcggagagc aattcgccgc gcttcagccg cgccagcgcg
     2221 tcgacgatgc cgagcggcgt cgcgccgtcg atgggatcgg agacgtagcg gttgtaagcc
     2281 gcttgccgca ccgaggcggg gacgccggcg cgggcggtgt agaaagcccg gccgaccgaa
     2341 tattccgggc gccaggtgag gccggcaatg cccgcctgca gcagcttttc gcccgggccg
     2401 aagcggatgc cgtcgatgcc cttgcgggcg aggaagtcgc gcaccgcctc ggggccgccg
     2461 gcgcggcgca tgaccacgtc gttggcggtg ttgtcgctct gctggagcgc gcggtggagc
     2521 aggtcgctga cgttggtgtt gaacccgccg cccttgatca tggcgcggat cggctggtgg
     2581 aacaccgtca tgtcctggtg gcggaccgtc accgggctgt agatgtcgag ctcgccgcgg
     2641 tcgatcttgt cgagcgcggt cagcgccacc cagaatttgc tcacgctctg ctgcggaaag
     2701 tggcgcgcgc cgtcataatg gctgacccag ccggtctgga tgtccttgac cgcgatgccg
     2761 acgtcgccgt tgaagccgcg gccgataagg gcgacgcggc tgtcgaggct gcgctgcgcc
     2821 gacaggacgg cggggggcgt ggcggggaga tattgccgcg tgaagggctg gctggcgcgc
     2881 ggcgccgcgc gcggcggcgg cggatagctt tgcgccgctt tgctggtcgg aagcgtggcg
     2941 gcgagggtgc cggaaaggcc gatcacgccg acgagggcgg ccttggttgc gcggctcagc
     3001 ttccgccgac cggcaggcgt acgcgatact ttcacttgcc cctcctaacg ccttttaccc
     3061 gaacggtttt atatacccga ccggcaattg ctgtcagctg aattggtttt ggcgtgggat
     3121 caaccgttca tcctccgggc cgaggcgagt cgggggcgtg caactattcc gccagccact
     3181 catcctcggc acttccgatc ggcattcccg gcgggatttg cggggctcgc ttggggtcgg
     3241 cgagcacctt gtcgagcgct tcgcggtcgg cgagcagccg cgccagcccc cgcgcccaat
     3301 cctgctcggc ttcattgcct tcgcgcttct ggagcatcgc cgccagccgc cgcagccttt
     3361 ggtcgagcgc gggcgccagc gtcggcgaca aagccgggtc gcgctggacg cgggcgaggg
     3421 cgagcgcgac ggtggtggcg acgcgccgct gcacggcccc gcgggagtcg tcgccgcgct
     3481 ggtcggcgaa gctgtccgtg atcacccggt cgatcacctc gtgggggccg agcgcggcgc
     3541 cgtcgacgct attctaaatc tggaggcggt tgagccgctc gggcgcgagc agcgcgttca
     3601 aggtcacggt ggcggcggct tcgctggcgg cgagcgggtc gaacaccggc ccgccggcgg
     3661 tgcggaaaat ctcgatgtca tattgccggt ccggattgcc cgaccagccg gccgacagat
     3721 acgggaggag gccggcaggc acttcgagct cggccggacg aagggtcgcc agcagcgcct
     3781 cgagcgcgcg ccgctgcgcc gcctccgcca ccaaggtcgc ggcctcgcgc ccatcgccgc
     3841 gcagcgaata gctgaagtcg acgccgccga cgagcttggc ggcggcatcc acctggtagc
     3901 ggtggagcag ccagatcggc acgaacttgc ggcgaaggtt cgcgaccggc tcgaccccgg
     3961 ccaacgcgcc gacgccgaac cgctcgaccg cggcgcggcg gacggcagtc atgcgctcga
     4021 gctccgtggc cgggtcgcca aaatcgtccc agatcgcgcc ttgcggatgg ccgctaccgg
     4081 tcggccgcgc gtcattgtcg gcgacaaagc gcagcccttc ggcgacggcg gcggcggcct
     4141 tggtaagggc ttccgcctcg ctgtcggtgc cgtagagcca gtcgaccgcg aacatgtccc
     4201 agc
//
LOCUS       DASVRK010000142        11413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_400023, whole genome shotgun sequence.
ACCESSION   DASVRK010000142 DASVRK010000000
VERSION     DASVRK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11413
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400023"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="VF589_05530"
     CDS             complement(<1..1582)
                     /locus_tag="VF589_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX8257072.1"
                     /translation="MRLFLLLPILALALPAAAQNSMPAGPVAPETDNPARDIPYPGTL
                     TLDVDATDTARGIFRVRETIPVVASGPITLRYARWLPGNHAPTGPIEAVAGLRISAGG
                     RPLAWRRNPTDMYAFDVEVPAGAAALEVAFDYISSSPGDIGRVEMTPEMLNLQWEKVA
                     LYPAGHYTRRIMIRASVTLPAGWTGVAALDGETRVGNRLSYGVTSYETLVDSPMFAGR
                     HYRRWDLGHNVDLNVFADRPEDLAATPEQIAVHRRLVDQTVKTFGSKHFDRYEFLLAL
                     SDELGGIGLEHHRSSENAVPRNYFTDWENTGSTRGLLPHEFTHSWNGKFRRPADLWTP
                     DYRTPMRNSLQWVYEGQTSYWDIVLGARSGVVPMDVALGEIASHAAYYTLQAGRSWRP
                     LADTTNQPILAYGQAAAYPTWQRGGDYYSESALIWLDVDTLIREKTGGRRSLDDFARA
                     FFGISNGDWGEITYTFEDLVAALNRVAPHDWDGFLRARVERPAPPPVEGIARGGYRLV
                     WREEPNAYDASAMKRSRSL"
     gene            complement(1653..2192)
                     /gene="ppa"
                     /locus_tag="VF589_05535"
     CDS             complement(1653..2192)
                     /gene="ppa"
                     /locus_tag="VF589_05535"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX8257073.1"
                     /translation="MNIDLIPIGDDPPNKVNVIIEVPVGGEPVKYEFDKKSGAIWVDR
                     ILHTPMRYPTNYGFIPHTLSPDGDPLDAMVVARSPFIPGSVVRVRPIAVLLLEDEAGG
                     DEKVLCVPVDTTFPYYQDVEEAEDLPSIVRQQIEHFFTHYKDLEAEKWVRVGHWLGRE
                     DACRIIAEAIERAKAAKAA"
     gene            2282..3598
                     /gene="hisS"
                     /locus_tag="VF589_05540"
     CDS             2282..3598
                     /gene="hisS"
                     /locus_tag="VF589_05540"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX8257074.1"
                     /translation="MSKGPQRIRGTQDIWGEEADRFRAVVEAFDRVRRLYAFQRVELP
                     VFEATEVFARSIGETTDIVSKEMYTFPDRGGDMLTLRPEFTAGICRAYLSEGWQQHAP
                     MKLATDGPVFRYERPQKGRFRQFHQLDAEIIGTDAPAADVELLALADQLLRELGIAEG
                     VTLQLNTLGDAATREAWRAALVDHFEAHRGELSEDSLTRLDKNPLRILDSKDPRDRPI
                     ADAAPAIDDFLTAEASDFFAAVTAGLDAADVAWTRNSRLVRGLDYYRHTAFEFVTDCL
                     GAQGTVLAGGRYDGLIESLGGPHTPAVGWAAGIERLGMLTELPPSGKMDVALVPLGPA
                     AEAAAVGMAANLRRHRIACEMAYKGNMKKRMSAAGKSGAVIALILGDEELARGEVTMK
                     DLASGAQSSLSLDDRFAEAVEGQLAELARGPLAGWTIYAPERTSSA"
     gene            3595..4671
                     /gene="prfA"
                     /locus_tag="VF589_05545"
     CDS             3595..4671
                     /gene="prfA"
                     /locus_tag="VF589_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX8257075.1"
                     /translation="MTSISDARIAQIEARRDELQNAMAAPDLAPDAFVKLSKDYAEIE
                     PVAAAAREVRRLRAELDVLAGMADDPEFRDMAAEEAEAIRRKLPEAERGLALKLLPRD
                     AADDRPAMLEIRAGTGGDEAALFAGDLFRMYQRYAEEQGWRVETISASPSDVGGFKEV
                     VASVTGQGVFARLKFESGVHRVQRVPATESGGRIHTSAATVAVLPEAEDVDVQIDEAR
                     DLRIDVYRSSGPGGQSVNTTDSAVRITHLPSGLVVIQQDEKSQHKNKAKALKVLRTRL
                     YELERERLASERAGARKSMVGSGDRSERIRTYNFPQGRVTDHRINLTLHRLPEILAGP
                     GLGELVSALIAEDEAERLASLDEQ"
     gene            4705..5517
                     /gene="prmC"
                     /locus_tag="VF589_05550"
     CDS             4705..5517
                     /gene="prmC"
                     /locus_tag="VF589_05550"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX8257076.1"
                     /translation="MREALAAATRRLAATSDTPRLDAELLMARALGVEREALLLSPLE
                     TDAPDAFEPLLARREAGEPIAYISGRRAFWTIELEVGPGVLIPRPDSETLIEAALAHF
                     GAPGPATILDLGTGPGTLLLASLDQWPRARGAGIDGSQAALRIAERNAERLGLGGRAQ
                     FRLGDWGEGIAERFDLILCNPPYVEAGAELPRDVADWEPKEALFAGPDGLDEYRRLAP
                     QIGPLLALGGIACIEIGAGQADAASALFAAEGLTVRLRRDLGGRLRCLVVSS"
     gene            5768..6568
                     /locus_tag="VF589_05555"
     CDS             5768..6568
                     /locus_tag="VF589_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEX8257077.1"
                     /translation="MINIRQNGRRRGRGGGPRSPNNTPNPGNRQDNRSRGNAVQLHEK
                     YKALARDAQLSGDRVQTEYFLQFADHYFRVLSENRPRFEEQRPRRDDFNSFDDEDQDD
                     GVQADGQDDGEDRNDRNDRDGRFDREPQRPQRESRFDREPRAEREQRAERGPRADRED
                     RPSREDRPGREDRSDREDRGEREQQQAEAEAQPEREARTGGLRPRRPRPRRDRADEQP
                     EANGDERISLEVLPPAIAPVGDGGEAPKPRRRTRRPAADGPDEIAPAA"
     gene            6983..8302
                     /locus_tag="VF589_05560"
     CDS             6983..8302
                     /locus_tag="VF589_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX8257078.1"
                     /translation="MKTISLAGLLAATALTAAAPARAQDAPADIAAELAAMRAKIDSL
                     EAKVKELEGAKAAAVPSWKGAPQFSDKESGWSFKPRGRLQFDAGYVESPDGVDDRGLG
                     FGTELRRGRLGVEGTIPGGFGYKFEIDFADNEVEVTDALLSYDFAENAGLVVGQHNPF
                     QSLEELTSSRFTSFMERAAFTDAFNFERRVGVSLGYANGPLIAQVGVFSDNIGDLAEA
                     GDENNSRSIDARLVYAPKLGETQLHLGGSAHFRTNDDLVDLEATTRYRQRPFIHTTDT
                     RFLATPSLAVGKESHYGLEAAAIRGPWHAAGEVHWLKANLIDDGNDPTFFGGYAEVGY
                     YLTGETRGYKAGAFDRTKVLRPVSDGGIGAFQVNLRYDYLDLIDQGVVGGKQSGYQAS
                     LIWIPQDHVRFLLNYGHLRYDDAAVVPVDGDNDYGINVLGARAQVDF"
     gene            8379..9845
                     /locus_tag="VF589_05565"
     CDS             8379..9845
                     /locus_tag="VF589_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX8257079.1"
                     /translation="MRYLLSISAALAAPALAQSAPGELKVTIYNDNLGLVQDTRQLNI
                     PAGRSRQEFPDVSAQIRAETVSFAAAGAGIVEQNFDYDLLSPTKLMEKAVGKTVTIVR
                     INPATGSETREQAKILSTNGGAVLQIGNRIEVLRDDGLPVRVIFDQVPENLRARPTLS
                     VTVESSRAGTRPARLTYLTPGLGWRSDYVALFDEAAGRLNLQGWITLTNSTGTAYRDA
                     DVVMVAGTPSRERDDQDSRRYRPENDSLRSAGTESGDRARLGEYYLYPLAERTTIADA
                     QTKQVSFLDVEGVGAGKGYEFRAEWLETSDTPRSASTVIKFANSQKSGVGDQLPAGVV
                     RVYLKDMRGQPQFVGESRIPHTPMGSELAVRTGEAFDVKVKAVVEKREKLRSDQWELS
                     ERYRISDSRSGSYVRTVETNPTFYRTTMVYTLTNARPAPVTVDLVQAGLDRGGFRDTR
                     VPSETIKGRQRSIDERLYLVSVPGNSETVIRATYDTRY"
     gene            9848..>11413
                     /locus_tag="VF589_05570"
     CDS             9848..>11413
                     /locus_tag="VF589_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257080.1"
                     /translation="MRTALSALLLLAAPGFAGAQPLVVSPAPEAVSVTVYRVHEGELD
                     LDWLGGYALVTETRTVDLPRGPSELRFEGVAGGIIPVSAIVAGLPGGVVEKNRDARLL
                     SPGRLIDASLGKRVHIRRTNRATGTVAETEAVLRSGPDGVVLETAEGIEALRCTGLPE
                     TLVYVSAPDGLTAKPTLAVRTATAAAARATVRLSYLATGFDWRANYVINMAPDRRSLD
                     LFAWLTLANGNDESFVAAQAMAVAGLPAREDEEEQGGSRVPAEIRLRCWPTGRTSDAA
                     PPELPHPSPPGEALEGEGEEAIVVTGSRVQEVKLAIPVAAIMAGQEELGDLKLYRIPE
                     PVTVAAHSQKQVALLARPGVRFERLYGAVLSALHYDDEEDPQPAEILLRLKNDKKRGL
                     GMPLPSGTAAIFESVGGRPMLAGEASVLDTAVGQELDLRVGEAAHVLIGQRRIQDPAE
                     REDDGDEVLPRRFEVEITNAEPRPVLVETLFRVSEGWRLVRPSRKLGVRDGRHMWRAK
                     VPAKGRLTLGYVME"
BASE COUNT         1879 a         3898 c         3919 g         1717 t
ORIGIN      
        1 cgaggctgcg gctgcgcttc atcgcggagg cgtcgtaggc gttgggctcc tcccgccaga
       61 cgaggcggta gccgccgcgc gcgatgccct cgaccggcgg cggcgccggg cgctcgaccc
      121 gggcgcgcag gaagccgtcc caatcgtgcg gcgccacgcg gttcagcgcc gccaccagat
      181 cctcgaacgt ataggtgatc tcgccccagt cgccgttgct gatgccgaag aaggcgcgcg
      241 caaaatcgtc gagcgagcgc cggccgccgg tcttctcccg gatcaaagta tcgacgtcga
      301 gccagatgag cgcgctttcg ctgtaatagt cgccgccccg ctgccaggtc ggataggccg
      361 cggcctggcc gtaggcgagg atcggctggt tggtggtgtc ggcgagcggg cgccagctgc
      421 ggccggcctg gagcgtgtaa taagccgcgt ggctcgcgat ctcgccgagc gccacgtcca
      481 tcggcaccac gcccgagcgg gcgccgagca caatgtccca atagctggtc tgcccttcat
      541 agacccactg caggctgttg cgcatcggcg tccggtaatc gggggtccag agatcggccg
      601 ggcggcggaa cttgccgttc cagctatggg tgaattcatg cggcagcagc ccgcgcgtcg
      661 atccggtatt ctcccagtcg gtgaaatagt tgcgcggcac cgcattttcg gagctgcgat
      721 gatgttcgag accgatcccg ccaagctcgt ccgacagcgc gagcaggaat tcgtagcggt
      781 cgaaatgctt ggacccgaac gtcttcaccg tctggtcgac cagccggcgg tgcaccgcga
      841 tctgctcggg cgtggcggcc agatcctccg gccggtcggc gaagacgttg aggtcgacat
      901 tgtggccgag gtcccagcgg cgataatggc ggccggcgaa catcgggctg tcgaccagcg
      961 tctcgtagct ggtgacgccg tagctcagcc tgttgcccac gcgggtctcg ccgtcgagcg
     1021 cggccactcc ggtccagccc gcgggcaggg tcaccgacgc ccggatcatg atccgccgcg
     1081 tataatggcc ggccggatag agcgcgacct tctcccactg caggttgagc atttcagggg
     1141 tcatctcgac ccggccgatg tcgcccggcg aggacgagat atagtcgaac gctacctcga
     1201 gcgcggccgc gccggccggc acctcgacgt cgaaggcgta catgtcggtc gggttgcgcc
     1261 gccacgccag cggccgcccg ccggcgctga tcctcagccc ggccaccgcc tcgatcgggc
     1321 cggtcggggc gtggttgccc ggcagccagc gcgcgtagcg gagcgtgatc ggtcccgacg
     1381 cgacgaccgg gatggtctcg cgcacccgga agatgccgcg ggcggtgtcg gtcgcgtcga
     1441 catccagggt gagggtgccg ggatagggta tgtccctcgc cggattgtcg gtctcgggcg
     1501 cgaccggccc cgccggcatg ctgttctggg cagcggccgg gagggccaga gcgaggatcg
     1561 gcagcagcag gaataggcgc acgggggatt ctccgaacgg gtgtaaggcg ttgccccgcg
     1621 atggcccaag cggcgccgcc cgtccagcct cgtcaggccg ccttcgccgc cttcgcccgc
     1681 tcgatcgcct cggcgatgat ccggcacgcg tcctcgcgcc ccagccaatg gccgacccgg
     1741 acccactttt cggcctcgag atccttatag tgggtgaaga aatgctcgat ctgctggcga
     1801 acgatgctcg gcaggtcctc ggcttcctcg acatcctgat aataagggaa ggtggtgtcg
     1861 accggcacgc acagcacctt ctcgtcgccg ccggcctcgt cttcgagcag cagcaccgcg
     1921 atcggccgca cccgcaccac cgagccgggg atgaagggcg atcgcgccac caccatcgcg
     1981 tcgagcgggt cgccgtcggg cgagagggtg tgggggatga agccgtaatt ggtcgggtag
     2041 cgcatcggcg tgtgcaaaat ccggtcgacc cagatcgcgc ccgatttctt gtcgaactca
     2101 tacttgacgg gctcgccgcc caccggcact tcgatgatga cgttgacttt gttcggcggg
     2161 tcgtcgccga tagggatgag gtcgatgttc atgggcgggc gctactccaa gagcgctgcc
     2221 agcgaaagcc gggatgacgg ctcagctttc cacgcgccgc aaagatgcta agggcgcggg
     2281 catgagcaag ggcccgcagc ggatcagagg cacccaggac atctggggcg aggaagccga
     2341 tcgcttcaga gcggtggtcg aggcgttcga ccgcgtgcgc cggctctacg ccttccagcg
     2401 ggtcgagttg ccggtattcg aggcgaccga ggtcttcgcc cgctcgatcg gcgagaccac
     2461 cgacatcgtc tccaaggaga tgtacacctt ccccgaccgc ggcggcgaca tgctgacgct
     2521 ccgccccgaa ttcaccgccg gcatctgccg cgcctatctg tcggaaggct ggcagcagca
     2581 cgcgccgatg aagctggcga ccgacggccc ggtgttccgc tacgagcggc cgcaaaaggg
     2641 ccgcttccgc cagttccacc agctcgacgc cgagatcatc ggaaccgatg ctccggccgc
     2701 cgatgtcgag ctgctcgctt tggccgacca actgctgcgc gagctcggca tcgcggaggg
     2761 cgtgacgctg cagctcaaca cgctcggcga cgcggcgacg cgcgaggcct ggcgcgcagc
     2821 cctggtcgac catttcgagg cgcacagggg cgagctttcc gaggacagcc tgacgcggct
     2881 ggacaagaac ccgctccgca tcctcgacag caaggacccg cgcgaccggc cgatcgccga
     2941 cgccgcgccg gcgatcgacg atttcctgac tgccgaagct tccgacttct tcgcggcggt
     3001 aaccgccggg ctcgacgcgg ccgatgtcgc ctggacgagg aactcgcggc tcgtacgcgg
     3061 cctcgattat tatcgccaca ccgccttcga gttcgtcacc gattgcctcg gcgctcaggg
     3121 gacggtgctc gccggcggcc gctatgacgg cctgatcgag agcttgggcg gcccgcacac
     3181 gccggcggtc ggctgggcag ctgggatcga gcggctgggg atgctgaccg agctgccgcc
     3241 ctcgggcaag atggacgtgg cgctggtccc gctcggcccg gcggccgagg cggcggctgt
     3301 cggcatggcg gcaaatctgc ggcgccaccg gatagcctgc gagatggcct ataagggcaa
     3361 tatgaagaag cgcatgtcgg ccgcgggtaa atcgggagcg gtgattgccc tcatccttgg
     3421 cgacgaagag ctcgctcgag gcgaggttac gatgaaggat ctcgccagcg gggcccaatc
     3481 cagtctctcg ctcgacgacc gtttcgcaga ggcggtagag gggcagctgg cagagttggc
     3541 tcgcggccca ttggccggtt ggaccatcta cgctccggag cggacctcct cggcatgacc
     3601 tccatctccg acgcgcggat cgcgcagatc gaggcgcggc gcgacgagtt gcaaaatgcg
     3661 atggcggcgc cggacctcgc gcccgacgcc ttcgtcaagc tctccaaaga ttatgccgag
     3721 atcgagccgg tcgccgccgc ggcgcgcgag gtgcggcggc tccgggccga gctcgacgtg
     3781 ctcgccggga tggcggatga ccccgagttc agggacatgg ccgccgagga agccgaggcg
     3841 atccgccgca agctgcccga ggccgagcgc gggctggcgc ttaagctgct gccgcgcgac
     3901 gccgccgacg accgcccggc gatgctcgaa atccgcgccg gcaccggcgg cgacgaagcc
     3961 gccttgttcg ccggcgacct gttccgaatg taccagcgtt atgccgagga gcagggctgg
     4021 cgggtcgaga cgatctccgc cagcccatcc gacgtcggcg gcttcaagga ggtggtcgcc
     4081 tcggtcaccg gccagggcgt gttcgcgcgg ctgaagttcg aaagcggcgt ccaccgcgtg
     4141 cagcgcgtgc ccgccaccga aagcggcggc cgcatccaca cctcggccgc tacggtcgcg
     4201 gtcctgcccg aggccgagga tgtcgacgtc cagatcgacg aggcgaggga cctgcggatc
     4261 gacgtctacc gctcgtcggg acccggcggc cagtcggtca acaccaccga cagcgcggtc
     4321 cgcatcaccc accttccgag cggcctcgtg gtgatccagc aggacgagaa gtcgcagcac
     4381 aagaacaagg ccaaggcgct gaaggtgctt cggacgcgcc tctacgagct cgagcgcgag
     4441 cggctcgcga gcgagcgcgc cggcgcgcgc aagtcgatgg tcggctcggg cgaccggtcg
     4501 gagcgcattc gcacctataa tttcccgcag ggtcgggtca ccgaccaccg catcaacctc
     4561 accttgcacc ggctgccgga aatcctcgcc ggccccgggc tgggcgagct cgtgtcggcg
     4621 cttatcgccg aggatgaggc cgagcggctg gcgagcctgg atgagcaata gccgccttcc
     4681 cgtgaaggcg agggcggagt gaaggtgcgc gaggcgctgg cggcggcgac acggcggctc
     4741 gccgcgacca gcgatacgcc ccggctcgac gccgaactgc tgatggcgcg tgcgctcggg
     4801 gttgagcgcg aggcgctgct cctttcaccg ctggaaacgg atgcgcccga cgcgttcgag
     4861 ccgctgctcg cgcggcggga ggcaggggag ccgatcgctt atataagcgg ccggcgcgcc
     4921 ttctggacca ttgagttgga agtgggcccc ggcgtcctca tcccacggcc cgacagcgag
     4981 accctgatcg aagccgcgct cgcccatttc ggcgcgccgg gtcccgccac gatcctcgat
     5041 ctcggcaccg gcccgggcac cttgctgctc gcatcgctcg atcaatggcc gcgcgcgcgc
     5101 ggcgcgggca tagacgggtc gcaggcggcg ctccggatag cagagcgaaa cgccgagcgg
     5161 ctgggccttg gcgggcgcgc acagttcagg ctcggcgact ggggcgaggg catcgcggag
     5221 cggttcgacc ttatcctgtg caacccgcct tatgtggaag ccggcgccga gctgccgcgc
     5281 gatgtcgccg attgggagcc gaaggaggcg ctgttcgccg gaccggacgg gctcgatgag
     5341 tatcgtcgcc tggcgccgca gatcggccct ctgctcgcct tgggcgggat cgcctgcatc
     5401 gagatcggcg ccggccaggc cgatgcggcg tccgctttgt tcgcggcgga agggctcacc
     5461 gtccgccttc ggcgcgacct tggcgggcgg cttcgttgct tggtcgtatc gagttgatcc
     5521 agcgcaacat ctttccgctt ggatttaccc gcgcaaatag ctacataggg cgcagggacg
     5581 gggcctgaca cagcttgaac gcgtgaggct agccgtcgcc gacccccgac tgcttcggcg
     5641 tccttggacc gcccggcgtg aggaggcgag agcttcgcgg ctccgccata caggtcgcgc
     5701 aaaatcaata cggccgggaa gggcccggcc ggcgtggcaa gagagaccac cagacgaggg
     5761 cttacgcttg attaacattc gtcagaacgg ccgcaggcgt ggccgtggcg gcggaccccg
     5821 ctcgccgaac aatacgccga acccgggtaa ccgccaagat aatcgctcgc gcggcaatgc
     5881 cgtccagctt cacgaaaagt acaaagcgct cgctcgcgac gcgcagcttt ccggcgaccg
     5941 cgtccagacg gaatatttcc ttcagttcgc ggaccattat tttcgtgtgc tgagcgagaa
     6001 ccgcccgcgc ttcgaagagc agcggccgcg gcgcgacgac ttcaacagct tcgacgacga
     6061 ggaccaggac gacggcgtcc aggccgacgg tcaggacgac ggcgaggatc gcaatgatcg
     6121 caatgatcgc gacggccggt tcgaccgtga gccgcagcgg ccccagcggg agtcgcgctt
     6181 cgaccgcgag ccccgggcgg agcgcgagca gcgcgccgag cgcggccccc gcgccgaccg
     6241 cgaagaccgg cccagccgcg aagatcgtcc cggccgcgaa gatcggtccg accgtgaaga
     6301 ccgcggcgag cgcgagcagc agcaggcgga agccgaagcc cagcctgagc gcgaggcgcg
     6361 caccggcggc cttcgtccgc gccgcccgcg tccgcgccgc gaccgcgccg acgagcagcc
     6421 ggaagcgaat ggcgacgagc ggatctcgct cgaagtgctt ccgccggcga tcgccccggt
     6481 cggcgatggc ggcgaggctc cgaagccgcg ccgccgcacc cgccggccgg ccgccgacgg
     6541 accggacgag atcgctccgg ccgcctgacc ggcttcatcg aattacagga tgggcggcgc
     6601 tctccggggc gcttggccag gccggcgctg aagcgccagc ctgcggtctg gacccctgcc
     6661 ttcgcagggg aacaggtacg cttcgcaggg gaacaggtgc gccactcgtg cccctgcgaa
     6721 ggcaggggcc cagaccaggc gaagcctgtc gaaccagggt cgggtagagg tcgcgcagtc
     6781 cgcgcatctg tgccgttgcg gccgctccca tgggtgtgaa cacatcgaca ttcatccagg
     6841 gcgccgccct ttctcgttcg ggcttggctc ccgtaacaat aatgtcaccc aaacgtcttc
     6901 cggctgtcac cgatctgtct tagcccggcc ttgagcggcc ccggcaaagg gcggggcatt
     6961 ggtgcgagac gggggaccga tcatgaagac aatcagcctg gcgggcttgc tcgccgccac
     7021 tgcgcttacc gccgccgcgc ccgcgcgcgc ccaggatgct cccgcggaca tcgccgccga
     7081 actcgccgcg atgcgcgcca agatcgacag cctcgaggcc aaggtgaagg agctcgaagg
     7141 cgccaaggcc gccgccgtgc cgagctggaa aggcgcgccg caattcagcg acaaggaaag
     7201 cggctggtcg ttcaagccgc gcggacggct ccagttcgac gccggctatg tcgagagccc
     7261 cgacggcgtc gacgatcgcg gcctcggctt cggcaccgag cttcgccgcg gtcgcctcgg
     7321 cgtcgaaggc accatcccgg gcggcttcgg ctacaagttc gagatcgact tcgccgacaa
     7381 cgaggtcgag gtcaccgacg ccttgctgtc ctacgacttc gccgaaaatg cgggcctggt
     7441 cgtcggccag cacaatccgt tccagtcgct cgaggagctg accagctcgc gcttcacctc
     7501 cttcatggag cgcgccgcct tcaccgatgc gttcaacttc gagcgccgcg tgggcgtctc
     7561 gctcggctat gccaacggcc cgctgatcgc gcaggtcggg gtcttttccg acaatatcgg
     7621 cgacctcgcc gaagccggcg acgagaataa ttcgcgcagc atcgacgcgc gcctcgtcta
     7681 cgcgcccaag ctcggcgaga cccagctcca cctcggcggc tcggcgcatt tccgcaccaa
     7741 cgacgatctc gtcgatctcg aggcgaccac ccgctaccgg cagcggccct tcatccacac
     7801 caccgatacc cgcttcctcg ccacccccag cctcgcggtc ggcaaggaga gccattacgg
     7861 cctggaggcg gcggcgatcc gcggcccctg gcatgccgcc ggcgaggtgc attggctgaa
     7921 ggcgaacctc atcgacgacg gcaacgatcc caccttcttc ggaggctacg cggaggtcgg
     7981 ctattatctg accggcgaga ctcgcggcta caaggccggc gccttcgatc gcaccaaggt
     8041 cctccgcccg gtctccgacg gcggcatcgg cgccttccag gtcaatttgc gctacgatta
     8101 tctcgacctc atcgaccagg gcgtcgtcgg cggcaagcag agcggctacc aagcctcgct
     8161 gatctggatc ccgcaggacc atgtccgctt cctcctcaat tacggccacc ttcgctacga
     8221 cgatgcggcg gtggtgccgg tggacggcga caatgattac ggcatcaacg tgctcggcgc
     8281 ccgggcacag gtcgacttct aagtcgctcc ggccgcgagg gattgacagg acgggccgcc
     8341 gcgtaacacg cttcccccgg gtcgaggggg gcctcgtcat gcgttacctg ttatcgattt
     8401 cagcggccct ggccgcgccg gctttggctc aatcggcccc gggcgagctc aaggtcacca
     8461 tctataacga caatctcggg ctggtgcagg atacgcgtca gctcaacatc ccggccgggc
     8521 gctcgcgcca ggaattcccc gacgtgtcgg cccagatcag ggccgagacc gtctccttcg
     8581 ccgccgcggg ggccggcatc gtcgagcaga atttcgatta cgaccttctc tcccccacca
     8641 agctgatgga aaaggcggtc ggcaagaccg tcaccatcgt ccgcatcaac cccgccaccg
     8701 gcagcgagac ccgcgaacag gcgaagatcc tttccaccaa tggcggcgcc gtcctccaga
     8761 tcggcaaccg catcgaagtg ctgcgggacg atggccttcc cgtgcgggtg atcttcgacc
     8821 aggtgcccga gaatctgcgc gcgcgcccga cgctgtcggt gacggtcgag agcagccgcg
     8881 cgggcacccg gccggcgcgg ctcacctacc tcacgcccgg gctcggctgg cggtccgact
     8941 atgtcgcctt gttcgacgaa gcggcgggac ggctcaacct gcagggctgg atcacgctca
     9001 ccaacagcac cggaaccgct tatcgggacg ccgacgtcgt gatggtcgcg ggaacgccca
     9061 gccgcgaacg cgacgaccag gacagccgcc gctatcgccc ggagaatgac agtctccggt
     9121 cggccggaac cgagagcggc gaccgggccc ggctcggcga atattatctc tatccgctgg
     9181 ccgagcggac cacgatcgcc gacgcgcaga ccaagcaggt gagcttcctc gacgtcgagg
     9241 gggtcggtgc cggcaaggga tatgagttcc gcgctgagtg gctcgagacg agcgacacgc
     9301 cccgcagcgc atcgaccgtg atcaagttcg ccaacagcca gaagtccggc gtcggcgatc
     9361 agctgcccgc gggcgtggtc cgggtctacc tcaaggatat gcgagggcag ccgcaattcg
     9421 tcggtgaaag ccggatcccg cacaccccga tgggctccga gctcgcggtc cgcacgggcg
     9481 aagcgttcga cgtcaaggtc aaggcggtcg tggagaaacg cgagaagctc aggagcgacc
     9541 agtgggagct cagcgaacgc taccggatca gcgattcgag aagcggaagc tatgtgagga
     9601 cggtggagac caatcccacc ttctatcgga ccaccatggt ctacacgctg accaacgcgc
     9661 gccccgcgcc ggtgacggtc gatctggtgc aggccgggct cgaccgcggc ggcttccgcg
     9721 atacccgcgt ccccagcgaa accataaagg gccggcaacg ctcgatcgac gaacggctct
     9781 acctcgtcag cgtcccgggc aatagcgaga cggtgatccg cgcgacttac gacacccgat
     9841 actgatcatg cgcacggcgt tatcggcgct gctgctgctt gcggcgcccg gcttcgccgg
     9901 ggcgcagccg ctcgtcgttt ctcccgcgcc ggaggcggtg tcggtcacgg tctatcgcgt
     9961 ccatgagggt gagctcgatc tcgactggct gggcggctat gcgcttgtga ccgaaacccg
    10021 aaccgtcgat cttccgcggg ggccgagcga acttcggttc gagggcgtgg ccggcggcat
    10081 catcccggtc agcgcgatcg tggcagggtt gcccggcggc gtggtcgaaa agaaccgcga
    10141 cgcgcgcctg ttgtcccccg gccgcctgat cgacgccagc ctcggcaagc gcgtccacat
    10201 ccgccgcacc aaccgcgcga cgggcacggt tgccgaaacg gaagcggtgc ttcgttcggg
    10261 accggacggc gtcgtgctcg aaacggccga aggtatcgag gcgctccgct gcacggggct
    10321 gccggaaact ttggtctacg tttccgcgcc ggatggtctt accgccaagc cgacgctggc
    10381 cgtgagaacg gccacggcgg cggcggcccg cgccaccgtc aggctctcct atctggcgac
    10441 cgggttcgac tggcgcgcca attatgtgat caacatggcg ccggaccgcc gaagcctgga
    10501 cctgttcgcc tggctcaccc tcgctaacgg caacgacgaa agcttcgtcg ccgcgcaggc
    10561 catggcggtg gccgggctcc cggcgaggga ggacgaggag gagcagggcg gctcgcgcgt
    10621 gccggctgaa atccggctcc gctgctggcc gaccggccgg acaagcgacg ccgcccctcc
    10681 ggagctgccc catccgtccc ctccagggga agcgctggag ggcgagggcg aagaagccat
    10741 cgtcgtgacc ggcagccggg tgcaggaggt gaagctggcg atcccggtcg ccgcgatcat
    10801 ggccggccag gaagagctgg gcgacctcaa gctgtaccga attccggagc cggtcacggt
    10861 ggcggcccat tcgcaaaagc aggtggcgct gctcgcccgg cccggcgtca ggttcgagcg
    10921 cctctacggc gccgtgctct ccgcgcttca ctacgatgat gaagaggatc cgcagccggc
    10981 ggagatcctg ctccggctga agaacgacaa aaagcggggg cttggaatgc cattgccttc
    11041 cggcaccgcg gcaattttcg agtccgtcgg cgggcggccg atgctcgccg gcgaggcgtc
    11101 cgtgctggat accgcggtcg gacaggagct ggaccttcgc gtcggcgagg ctgcccatgt
    11161 cctgatcggc caacggcgga tccaggaccc ggctgagcgg gaggatgacg gtgacgaggt
    11221 gctcccgcgt cgcttcgagg tcgagatcac caacgccgag ccgcgcccgg tcctcgttga
    11281 gacgctgttt cgtgtgagcg agggatggcg gctggtgcgg cccagccgga agctgggcgt
    11341 ccgcgacggc cgccatatgt ggcgggccaa ggttcccgcg aaaggccggc tgacgctcgg
    11401 ctatgtgatg gaa
//
LOCUS       DASVRK010000143        21477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_275408, whole genome shotgun sequence.
ACCESSION   DASVRK010000143 DASVRK010000000
VERSION     DASVRK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 21477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21477
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275408"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VF589_05575"
     CDS             <1..1309
                     /locus_tag="VF589_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257081.1"
                     /translation="GDGTYATAEGRKTLVGVERVLVVGSGHDDRMSGGSDNDSLNGGQ
                     GNDMLKGGAGDDTLTGEAGADSLYGEDGDDTFLDSPLHGGPADRLLDGGAGIDRAQIY
                     LPDHTADIVFSIRNPARNANFGSTVVRNVEQVLLWAGSGDDHLTGGRLDDDLFSSAGD
                     DTLLGLGGRDDLYSGEGDDILSGGGGNDRLFGDAGRDRLSGGSGNDDLEGGDDHDLLI
                     GGAGGDRMDGGYGNDSYFVDSVSDEVVETAVDGGVDIVFASLSFTLGEGVEHLRLEGS
                     EGLTGLGNAADNDVEGTSGADTLYGQGGRDRIIGNDGDDRLDGGSGNDQLRGGAGSDQ
                     FVFTEILSAEDNVDALADFNVADDVILLWGRVFAGIGVGRLGSDAFHYGAEAADAEDR
                     ILYQQWTGRLFYDPDGSGRAEPILFARVEAGLELTAADFAVQS"
     gene            1360..3096
                     /locus_tag="VF589_05580"
     CDS             1360..3096
                     /locus_tag="VF589_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8257082.1"
                     /translation="MPDPADLWRTAFRHPTPWEQDFPPLSLPDMFEASARRVPNAWLI
                     DFMGRKYSYAETLHGVNRVACGLAKLGIQKGDRVGLFLPNVPHYAAAYYGALKLGATV
                     VNFSPLYSVAELVAQVEDSGTRLLFTLSAAALLPNALELLELTRLERLVVGSVAGALP
                     PAKSLLYRLFKRKEIAARPRDQRIMAFSALIANDGRCSAVPIDPVEDLALIQYTGGTT
                     GTPKGGMLTHQNLSANASQVNAIDPDPAAEDRIMGVLPLFHVFANTCVLNRTVLRGGE
                     MVLLPRFDAGQTLAAIERTRPTAMPGVPTMYQALLDHPKIGSTDLSSLRICISGGAPM
                     PSEVKRRFEEKTGARLVEGYGLTESSGVVSTNPYDGLNKTGTIGQPLPRTLVLLVDKE
                     DPSRPPAEGEPGEIVIAGPQVMKGYWRRPEADAEVFVAGGLRTGDVGLIDEDGYIRIV
                     DRLKDMIAVSGYKVFPSQIEEILYRHESVREALVIGIPDPYAGERPKAFVTLRDGADL
                     SGEALMLWLNPLLGKHERVVAVELRDALPKTMIGKLSRKELQAEERAKAGSDSVSGIN
                     PDRGGITPNRSG"
     gene            complement(3208..4101)
                     /gene="era"
                     /locus_tag="VF589_05585"
     CDS             complement(3208..4101)
                     /gene="era"
                     /locus_tag="VF589_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX8257083.1"
                     /translation="MSERCGLVAVVGAPNAGKSTLVNALIGQKVAIVSPKAQTTRVRL
                     MGIAILGEAQILLLDTPGIFEPRRRLDRAMVAAAWGGAQDADLIALVIDAKTGFSERI
                     EQMLDTLKGRREPKILILNKVDITPKERLLTLTARLSERLDFEEIFMVSATTGDGAAD
                     LKQALAARVPEGPWHFPEEQVSDATDRMLAAEVTREQLYLQLHAELPYASAVETEKYE
                     ERRDGSVAIHQQILVGRPTQRAIVLGKGGARIREIGAAARAELAELLGRKVHLFLHVK
                     VKPDWEEDRGLYRDIGLDWVE"
     gene            complement(4098..4769)
                     /gene="rnc"
                     /locus_tag="VF589_05590"
     CDS             complement(4098..4769)
                     /gene="rnc"
                     /locus_tag="VF589_05590"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX8257084.1"
                     /translation="MHAPDKTSAWIEATLGHRPGDPALYARALTHSSVGEDHYERLEF
                     LGDRVLGLAIATWLYETFPDEPEGKLSRRLNALVSGETCAEIAREIGANGQVKLGKQA
                     RDDGAADSDNVLGDVVESLIGALYLEAGLEAATAFIRRAWADRVSTHDKAPKHPKSAL
                     QEWAAANGRRAPVYQLTGRSGPHHAPKFVVEVEIAGVGAASAEGLSKQEAETAAAEAL
                     VAKLP"
     gene            complement(4769..5740)
                     /gene="lepB"
                     /locus_tag="VF589_05595"
     CDS             complement(4769..5740)
                     /gene="lepB"
                     /locus_tag="VF589_05595"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8257085.1"
                     /translation="MAHHSGHLAPREAANAGAIRLTPRMGHAKAGAMETSETMEAPAP
                     PPANGPALKSRRSRKRKKEGVAETTRFLLLLFLFAVVLRSFVVAPFSIPSGSMLPRMM
                     IGDYLFVAKWSYGYSRVSLPFGLGSFDGRFLAREPERGDVAVFRYPDGSDEDYVKRVI
                     GLPGDQVQMQEGVLYLNGEAVPRVRVADFLMPITPNSPCRTIDPAAARVVTDREGDRF
                     CAYPRYRETLPEGRSYYVLDQIEGQTDDTPVYIVPDDHYFMMGDNRDDSEDSRIALEA
                     GGVGYLPADYLIGEALITFFSTDGSAQWLKPWTWFSAARWERIGDTY"
     gene            5761..6174
                     /locus_tag="VF589_05600"
     CDS             5761..6174
                     /locus_tag="VF589_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257086.1"
                     /translation="MKSATDVMQEIIFSAVIDSFVAIKAASKGLPNTMVRELNAVHPN
                     TTLADLPPELQAAIAANVRAAFTRLLKEGYSVSPGRAAPSAPPPRTREGPRPGPGARR
                     PPPKRPGGDGPRSPRDGRPPRDGGRPPGRGPKPRG"
     gene            6249..7754
                     /gene="pgi"
                     /locus_tag="VF589_05605"
     CDS             6249..7754
                     /gene="pgi"
                     /locus_tag="VF589_05605"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX8257087.1"
                     /translation="MTDPWAAVEAAETRPLADLFAAEPDRLSRLVVEEAGIRFDFSKT
                     HLDAGLVAAFAALAEAHDLAGARDALFAGQAVNVTEGRSAEHVAERGSGSPESVARAQ
                     ALHARMRALIDAIEAGAFGPVSHILHIGIGGSALGPDFLIDALGRDSGRYDSAVVSNV
                     DGAALEAAFGRFDPAATMLVIASKTFTTSETMLNARSALAWLEEGGVADPYGRVVALT
                     ASPERAIEFGIDQTRILPFAESVGGRYSLWSSIGFPAALALGWAAFEELLEGAAAMDR
                     HFRLAAPAENAPLLAAFVDRYYANRRGAQTRAVFAYDERLRLLPSYLQQLEMESNGKS
                     VTVAGDPVGRATASVTWGGVGTDAQHAVFQLLHQGTHLVPVEFVAAIEPEHGLDFEHH
                     RQLLVNCFAQGAALMAGKPSDDPARAYPGDRPSTTILLDRLDPATLGALIAFYEHRTF
                     ANAVLLGINPFDQFGVELGKEMAKAMDGDGAIRFDPSTEALIARALGEGPQ"
     gene            7815..9161
                     /gene="gorA"
                     /locus_tag="VF589_05610"
     CDS             7815..9161
                     /gene="gorA"
                     /locus_tag="VF589_05610"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX8257088.1"
                     /translation="MMTYDYDLFVIGAGSGGVRAARVSAAHGAKVAIAEEHKIGGTCV
                     IRGCVPKKLLVYGAHFAEDLQDARRFGWEVPECSFNWPILRDNVLAEVSRIEGAYGEM
                     LDNSGVAIFHQRAVVTGPNGIRLADGTELAVRTILIATGARPQLPDFPGAEHGITSNE
                     VFHLDAIPARAVIAGGGYIANEFAGIFNEFTCDVTLVNRSDAILRQYDAQVRDRLLQI
                     SLMKGINFKFNAPFEKVDKQEDGTLVIQLKGQEPIEADMLLFAIGRAPNTEGLGLESA
                     GVEIDEYHAVVVDDDNKTACDSIYAVGDVTNRVQLTPVAIREGQVFADTVFGNKPTRV
                     DYANIPSAVFSHPPIAGVGMTESDARNRLGSVKTYVSDFRPMKNVLAGRNERSLYKLV
                     VNGETDQVVGIHMIGPDAPEILQAAAIAVKAGLKKEDFDRVIALHPTMAEELVLMR"
     gene            9292..10590
                     /gene="hmgA"
                     /locus_tag="VF589_05615"
     CDS             9292..10590
                     /gene="hmgA"
                     /locus_tag="VF589_05615"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX8257089.1"
                     /translation="MATNDYMSGFGSHFATESVPGALPQGRNSPQRPAFGLYAEQLSG
                     TAFTAPRHENRRSWLYRMRPSAEHPLYRRYEGAELFALRPAVTGELAPNRLRWSPLAL
                     PEAPTDLIDGLVTMVANRPPEDLEGVAVHIYRANRDMERRFFGNADGEMLFIPQLGRL
                     TLLTELGRIDVAPGEVALVPRGVRFRALLPDGEGRGYVAENHGSLFRLPDLGPIGANG
                     LANPRDFETPVAWYEDVDGEVELVQKYLGALWTTTLDHSPLDVVAWHGNLAPWKYDLE
                     RFNTIGSISFDHPDPSIFTMLTSPSDVPGRANADFVIFPPRWLVAEDTFRPPWFHRNV
                     MSECMGLIHGAYDAKAEGFAPGGLSAHNMMAGHGPDVASWKGASEAELKPHKIEGTMA
                     FMVESCWPFRPSDYALGCGELQKDYDEAWGGFPKAQLPPR"
     gene            complement(10635..11858)
                     /locus_tag="VF589_05620"
     CDS             complement(10635..11858)
                     /locus_tag="VF589_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX8257090.1"
                     /translation="MVRHSHLRGGTGQDGHRVSAMELFFDLVFVFAVTQLSHYLLEHH
                     SLIGAAQTLLMFLTIWWAWAFTAWATNWLDPDRSPVRLMLALLMLLSLVMSSAIPGAF
                     GPYGLPFALAYVAIQIGRTAYTSWAKGEWVRGASKNMTRATLYFVASAPLWIAGGLDP
                     DPSRRPAWWAAALALECAGPLLFFAVPGLGRSRAEDWQISGGHMAERCALFIIISLGE
                     GILVTGATFSELEPSRATILAFVSAFVASVAMWWIYFDIGARRGTELMEHDEHPGLIG
                     RAAYTYGHIPIVAGIVVLAVADEQVLMHPTGHSEPFLLACLLGGALLFIGGTMAFKRL
                     TSPIRRWPLSHLVGAALFALYGLWALVAHPEPLLLHLTATALFIVIAVWEWGSFHGGW
                     VERWARVRKPRRSAR"
     gene            complement(12051..12287)
                     /locus_tag="VF589_05625"
     CDS             complement(12051..12287)
                     /locus_tag="VF589_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HEX8257091.1"
                     /translation="MAGNPFTRHPKEIGESYGEHLGTAAGFGATMMVGGLCVLVHAIL
                     PFLFVETGSRTMDKLHRKMRKRADRVNWERHPII"
     gene            12378..13565
                     /locus_tag="VF589_05630"
     CDS             12378..13565
                     /locus_tag="VF589_05630"
                     /inference="COORDINATES: protein motif:HMM:NF006719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HEX8257092.1"
                     /translation="MFDRFPEAAGDALHAVMARFRADPRPHKIDLGVGVYRDADGAAP
                     VMPAVKAAEAVLLRDEDSKVYQALHGDDRFITAMTDLVFGADHPAVRDGRIAAIQATG
                     GTGALRIAFDAAAIAAPGARLHLGEPSWPNHAALAAAAGLALAPHSYFDVAAQAIDLA
                     AIGAAAGGAGEGDLFILHGPCHNPTGADLTLEQRRDLLSVLAGRGAVPVIDAAYYGLG
                     DGLDADLRILREVAAALPRVFIAISCSKVFGLYRERTGILFALCADPAERARVQGQLE
                     GIGRTLVSMPPAHGAGTVAIILGDEALRKLWRGELDRMRARIGGLRAELAALANVVPM
                     LRGIEAQRGIFRMLPLTREQTERLGRDHAIHMPPSGRINLAGLKRGDAARLAEALAAI
                     DPA"
     gene            complement(13572..13949)
                     /locus_tag="VF589_05635"
     CDS             complement(13572..13949)
                     /locus_tag="VF589_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257093.1"
                     /translation="MKRMRIAAAALALAAAGGTAAAQGFDGYDPEEARWIASEPGGQY
                     LQLSDTPLFRHDRLSRHNGYFDHGATNGRHFDYDRGYPYDWFNYGAEAEAESEPPRTM
                     RCETEWVRGRGEREDVPVQVCRG"
     gene            complement(14050..14706)
                     /locus_tag="VF589_05640"
     CDS             complement(14050..14706)
                     /locus_tag="VF589_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8257094.1"
                     /translation="MRDIIGAAFVSLDGVMQAPGGPEEDPTGGFELGGWSAPLSDDTI
                     GESMAPLFAEPFDLLLGRNTYDIFAAHWPYRDDPIGTVFNRAAKHVVTSSAEPLAWSN
                     SHAVSDGIDGVARLKEQDGPELRIWGSSKLYAGLLGRGLVDRLFLMTYPVILGKGKRL
                     FADGLPPQALKLVESKVSSTGVIIASYERDGPVQVGSFVPDEPSEAELARREKMKREG
                     "
     gene            complement(14699..15187)
                     /locus_tag="VF589_05645"
     CDS             complement(14699..15187)
                     /locus_tag="VF589_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8257095.1"
                     /translation="MSKLTPCLWFDGNAEEAANFYVSLLPDSRVDNVVRAPSDYPSGQ
                     AGNVLVVEFTLAGAKFTGLNGGPYFQFNEAVSFQIDCDDQAEVDRLTEALSAVPEAEQ
                     CGWVKDRYGLSWQIVPRVMKAMLADPDRAKAKRAMDAMMEMKRLDIAALERAFEGEAA
                     DA"
     gene            complement(15257..15700)
                     /locus_tag="VF589_05650"
     CDS             complement(15257..15700)
                     /locus_tag="VF589_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX8257096.1"
                     /translation="MIFGGFAGLLDEGSGRGPYVDGFVLPVMPDKKDAYLALARKAAG
                     DFEKHGALRLVEAWGDDIPDGKQTDFRRAVQAGEGENVVFSFIEWPDKATRDAGWAKM
                     MADPEMQPNHDDMPFDGKRMFWGGFDVVLDERAGAGAAAAERELA"
     gene            complement(15683..16015)
                     /locus_tag="VF589_05655"
                     /pseudo
     CDS             complement(15683..16015)
                     /locus_tag="VF589_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
     gene            complement(16041..16532)
                     /locus_tag="VF589_05660"
     CDS             complement(16041..16532)
                     /locus_tag="VF589_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8257097.1"
                     /translation="MSDTLELSVTRLIDAPPATVYRVYTERTAEWWVPKPWTTPAVEW
                     ELRTGGRMYTEMRGPDGEGEGGEGVFLEVVPNQRIVWTNAFKPGWQPQVQTNEGCDFA
                     MVAIVTFEPEGSGTRYTARVQHWDPEAVEKHREMGFEQGWTLVAAQLAELAEAEAGTA
                     IAA"
     gene            complement(16556..17314)
                     /locus_tag="VF589_05665"
     CDS             complement(16556..17314)
                     /locus_tag="VF589_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8257098.1"
                     /translation="MAASFIWYELMTSDTEAAKGFYRAVVGWETKAFEGGGDYTILEA
                     NGRGVGGLMPIPEDARQTGARPVWFGYIAVDDVDAEAARIVEAGGKLHKDIIDVPTVG
                     RIAMVADPQGAPFYLITPEGEAQAPVPPMSDGHVAWHELHTTDWEAAFDFYSSRFGWA
                     KAEAMDMGPMGIYQIVSADGPNWIGAMYNADTFGRPGWLYYFAVGDIDEAAERVRAAG
                     GELLEGPMEVPGNAWVVQCRDPQGALFALVGKRG"
     gene            complement(17343..17774)
                     /locus_tag="VF589_05670"
     CDS             complement(17343..17774)
                     /locus_tag="VF589_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8257099.1"
                     /translation="MSRMIFVNLPVTDLERSMRFYEAVGAANEPKFTNAAAAMMRFSE
                     AVNVMLLTHDFYRTFTTKQIPDAHKNAQMLLCISADSREAVDAQIETARAAGGVLDPT
                     PTQDYGSMYGRSFEDPDGHIWEVMWMDPAAAEQGAAAFEHA"
     gene            17854..18564
                     /locus_tag="VF589_05675"
     CDS             17854..18564
                     /locus_tag="VF589_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX8257100.1"
                     /translation="MKLQNVTESRRAVAKRRYDDACGTAHALDLIGERWALLVMRELM
                     LGPKRFSDIRADLPGISANVLTQRLEGLEAAGLLVRRRLPPPANAQVYELTPWGYEAE
                     PIVQSLGRWAARSPAHDPTLPISPVSLMLSFRTMFDAERARGLDARIGFRLGAESFLA
                     RIADGKLEVERAEPASADAIFTGTSAALAAAVYGGQPLPALAEAGLLAATGDQAVVQR
                     FLTLFPLPPKVSEAAPPA"
     gene            complement(19238..19570)
                     /locus_tag="VF589_05680"
     CDS             complement(19238..19570)
                     /locus_tag="VF589_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257101.1"
                     /translation="MGPPTGAEITGHSVQVETNGVVNTVYFDAGGSARIVSPAGREVP
                     AQWFVENQRLCLATGTTARECWAYQAAFQPGQAMSLTSDCGTSRWTAMSTATFAPPEP
                     PVRRGERG"
     gene            20020..20277
                     /locus_tag="VF589_05685"
     CDS             20020..20277
                     /locus_tag="VF589_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257102.1"
                     /translation="MGAALCLSSTSVYASSAAACQAGQAGCVLPVTDPAAAPAPAMET
                     SAPIIEEGTGIGILPIIAVLALAGLALILLLDDDEEENSPI"
BASE COUNT         3444 a         7486 c         7097 g         3450 t
ORIGIN      
        1 cggcgacgga acctatgcga cggccgaggg gcggaagacg ctggtcggcg tggaaagggt
       61 cctggtcgtc ggcagcggcc acgacgaccg catgtcaggc ggctcggaca atgattccct
      121 gaacgggggc cagggcaatg acatgctcaa gggcggagcg ggcgacgaca ccctgaccgg
      181 agaggccggt gcggattcgc tgtacggaga ggatggcgac gacactttcc tcgacagtcc
      241 gctccatggc ggtcccgcgg accgccttct ggacggtggc gccgggatcg accgggccca
      301 aatatacctg ccggatcaca cggcggacat agtgttcagc attcgcaacc ccgcccggaa
      361 cgcgaacttc ggctcgacgg tggtgcgcaa cgtcgagcag gttctgctgt gggccggctc
      421 aggcgacgac cacctgaccg gcgggcgtct ggacgacgat ctgtttagtt ccgccggaga
      481 tgacacgctg ctgggcctgg gcgggaggga tgacctgtat agcggcgagg gtgacgacat
      541 cctgagcggc ggtggcggca atgatcgcct gttcggcgat gcaggccgcg acaggctgtc
      601 gggcggctcg ggaaatgatg atcttgaagg gggggatgat catgacctgc tcatcggcgg
      661 agcaggaggt gatcggatgg acggaggtta tggcaacgac agctacttcg tcgacagcgt
      721 ctcggacgaa gtcgtcgaaa ccgctgtgga cggcggagtg gatatcgtct tcgcctcgct
      781 gagcttcaca ttgggtgaag gcgtcgaaca tcttcgcctg gagggaagcg aggggctgac
      841 cggcctcgga aatgccgccg acaacgatgt cgaggggact agcggcgccg acacgctcta
      901 cgggcagggc gggagggacc ggataatcgg aaatgacggc gacgatcggc tcgatggcgg
      961 gtccggcaac gaccagctgc ggggcggcgc cggttccgac cagttcgtct tcaccgaaat
     1021 actcagcgcc gaggacaatg tggacgcgct ggcggacttc aacgtcgcgg acgacgtcat
     1081 cttgctgtgg ggccgcgttt tcgccgggat cggcgtcggc cggcttggct ccgacgcctt
     1141 tcactacggg gccgaggctg cggatgccga ggaccggata ctctatcagc agtggaccgg
     1201 tcgccttttc tacgaccccg atggcagcgg gcgcgccgaa ccgattttgt tcgcgcgggt
     1261 cgaggccggg ctggagctca ctgcggcgga cttcgccgtc cagtcgtgat gacgccggtt
     1321 ggagttgccg ggccgataga cgcccgctaa cgcttccacg tgcccgaccc cgccgacctg
     1381 tggcgcaccg cctttcgcca tcccacgccc tgggagcagg acttcccgcc gctgagcctg
     1441 cccgacatgt tcgaggcctc ggcgcggcgc gtgcccaatg cctggttgat cgacttcatg
     1501 ggccgaaaat acagctatgc cgagacgctg cacggggtga accgcgtcgc ctgcgggctg
     1561 gcgaagctcg gcatccaaaa gggggaccgg gtcggcctgt tcctgcccaa cgtgccccat
     1621 tatgccgcgg cttattatgg ggcgctgaag ctcggcgcga ccgtggtcaa cttctcgccg
     1681 ctctactccg tcgccgagct ggtggcgcag gtcgaggata gcggcacccg gctgctcttc
     1741 acgctctcgg ccgccgcctt gctgcccaat gcgctggagc tgctcgagct caccaggctc
     1801 gagcggttgg tggtcggctc ggtcgcgggc gcgctgccgc cggccaagtc gctgctctac
     1861 cggctgttca agcgcaagga gatcgccgcc cgcccgcgcg accagcggat catggctttc
     1921 tccgcgctga tcgccaatga cggccgctgc tcggccgtgc ccatcgaccc cgtcgaggat
     1981 ctggcgctga tccaatatac gggcggcacc accggcacgc ccaagggcgg catgctcacc
     2041 caccagaatc tgtccgcaaa tgcgagccag gtgaatgcga tcgaccccga tcccgcggcc
     2101 gaggatcgga tcatgggcgt gctgccgctg ttccacgtct tcgccaacac ctgcgtcctc
     2161 aatcgcaccg tgcttcgcgg cggcgagatg gtgctgctgc cgcgcttcga cgccggccag
     2221 acgctcgccg cgatcgagcg cacccgcccg accgcgatgc ccggcgtccc gaccatgtac
     2281 caggcgctgc tcgaccatcc caagatcggg agcaccgatt tgtcctcgct gcgcatctgc
     2341 atttccggcg gcgcgccgat gccgtcggag gtcaagcgcc ggttcgagga gaagaccggc
     2401 gcccgcctgg tcgaaggcta tggcctcacc gaaagctcgg gcgtggtgtc gaccaaccct
     2461 tatgacgggc tcaacaagac cggcacgatc ggccagccgc tgccgcgcac gctcgtcttg
     2521 ctcgtcgaca aggaggaccc gtcgcggccg ccggccgaag gcgagcccgg cgagatcgtg
     2581 atcgccgggc cgcaggtgat gaagggctat tggcggcgcc ccgaggccga tgccgaagtc
     2641 ttcgtcgccg gcggccttcg caccggcgac gtcggcctga tcgacgagga cggctatatc
     2701 cgcatcgtcg accggctgaa ggacatgatc gccgtctccg gctacaaggt cttcccgagc
     2761 cagatcgagg agatactcta ccggcacgaa tcggtgcggg aagcgctcgt gatcggcatt
     2821 cccgatccct atgccggcga gcgtcccaag gcgttcgtga ccttgcgcga cggcgcggac
     2881 ctgagcggcg aggcgctgat gctctggctc aaccccttgc tcggcaagca tgagcgcgta
     2941 gtcgcggtgg agctgcgcga cgcgctgccc aagacgatga tcggcaagct gagccgcaag
     3001 gaactgcagg cggaggaacg ggccaaggcc ggcagcgata gcgtgagtgg aatcaacccg
     3061 gaccgtggtg gtatcacccc gaaccgttcg ggctgagcct gtcgaagccc tgcttttccc
     3121 ttccccggcg gggaagaagg gcccttcgac aagctcaggg cgaacggtgc gggtggatca
     3181 attctggtgc tcttggccga accgccctca ctccacccag tcgagcccga tgtcgcggta
     3241 gaggccgcga tcctcctccc agtccggctt caccttgacg tggaggaaca ggtggacctt
     3301 gcggccgagc aattcggcca gctcggcgcg ggcggcggcg ccgatctcgc ggatgcgggc
     3361 gccgcctttg cccaaaacga tcgcgcgctg cgtcgggcgg ccgaccaata tctgctggtg
     3421 gatggcgacc gagccgtcgc ggcgctcctc atatttctcg gtctcgacgg cgctcgcata
     3481 aggcagctcg gcgtggagct ggaggtagag ctgctcgcgg gtgacttcgg cggcgagcat
     3541 gcggtcggtg gcgtccgaca cctgctcctc cgggaaatgc cagggccctt ccggcacccg
     3601 cgcggctagc gcctgcttca agtccgcggc gccgtcgccg gtagtcgccg agaccatgaa
     3661 gatctcctcg aagtcgagcc gctccgaaag ccgcgccgtc agcgtcagca atcgctcctt
     3721 gggcgtgatg tccaccttgt tgaggatcag gatcttgggc tcgcggcggc ccttgagcgt
     3781 gtcgagcatc tgctcgatcc gctccgaaaa gccggtcttg gcgtcgatca ccagagcgat
     3841 gaggtcagcg tcctgcgccc cgccccaggc ggcggcgacc atggcgcggt cgagccgccg
     3901 ccgcggctcg aagatgccgg gcgtgtcgag cagcaggatc tgcgcttcgc caaggatggc
     3961 gatccccatc agccgcaccc gcgtggtctg cgccttgggg ctgacgatcg cgaccttctg
     4021 gccgatgaga gcattgacga gcgtcgactt gcccgcattg ggcgcgccga ccacggcgac
     4081 gaggccgcac cgctcgctca tggcagcttc gccaccaaag cttcggccgc cgccgtctcc
     4141 gcctcctgct tggacagccc ttccgcgctg gcggcgccga cgccggctat ctcgacctcg
     4201 accacgaatt tcggcgcgtg gtgcggcccg gaccggccgg tgagctggta gacgggcgcg
     4261 cgccggccgt tggcggcggc ccattcctgc agcgccgatt tgggatgctt gggcgccttg
     4321 tcatgggtgc tgacgcgatc ggcccaggcg cggcggatga aggccgtcgc cgcctccagc
     4381 cccgcctcca gataaagcgc gccgatcagc gactccacga cgtcgccgag cacgttgtcg
     4441 ctgtcggcgg cgccgtcgtc gcgggcctgc ttgccgagct tcacctgccc gttggcgccg
     4501 atctcgcggg cgatctccgc gcaggtctcg ccggagacca aggcgttgag ccggcgcgaa
     4561 agcttgccct cgggctcgtc ggggaacgtt tcgtagagcc aggtggcgat ggccagcccg
     4621 agcacccggt cgccgaggaa ttccagccgc tcgtaatgat cctcgccgac gctcgaatgg
     4681 gtgagcgcgc gcgcatagag cgccggatcg ccggggcggt ggccgagcgt cgcctcgatc
     4741 caggcggaag tcttgtcggg cgcgtgcatc agtatgtgtc cccgatccgc tcccagcgcg
     4801 cggctgagaa ccacgtccac ggcttcagcc attgggccga accgtcggtc gagaagaagg
     4861 tgatcagcgc ctcgccgatc agatagtcgg ccgggagata gccgacgccg cccgcctcca
     4921 gcgcgatccg gctgtcctcg ctatcgtcgc gattgtcgcc catcatgaaa taatgatcgt
     4981 cggggacgat atagaccggg gtgtcgtcgg tctggccctc gatctggtcg agcacataat
     5041 aagagcggcc ctcgggcagc gtctcgcggt agcggggata ggcgcagaaa cggtcgccct
     5101 cgcggtcggt caccacccgc gccgcggccg ggtcgatcgt ccggcacggg ctgttgggcg
     5161 tgatcggcat caggaaatcg gcgacccgca cccgcggcac cgcctcgccg ttcaggtaca
     5221 atacgccctc ctgcatctgc acctgatcgc ccggcaggcc gatcacccgc ttcacataat
     5281 cctcgtcgct gccgtcggga tagcggaaca ccgcgacgtc gccgcgctcg ggctcgcgcg
     5341 cgaggaagcg gccgtcgaac gagccgaggc cgaacggcag gctgactcgc gaatagccat
     5401 agctccattt ggcgacgaac agatagtcgc cgatcatcat ccgcggcagc atcgatccgg
     5461 acgggatgct gaacggcgcg acgacgaagc tgcgcagcac gaccgcgaac aggaacagca
     5521 gaagcaggaa gcgcgtcgtc tccgcgaccc cctccttctt ccgcttgcgg cttcgcctgc
     5581 ttttcaacgc cggcccattg gcgggcgggg gcgccggcgc ttccatggtc tcgctcgtct
     5641 ccatcgcgcc tgctttcgcg tggcccatgc gcggggtcaa gcggatcgca ccggcattgg
     5701 cggcttcgcg cggcgcgaga tggccgctat ggtgcgccat cggaggagcc ggagaaggtc
     5761 atgaagtcag caacggacgt gatgcaggag atcatctttt cggcggtgat cgattcgttc
     5821 gtcgcgatca aggcggcctc gaaggggctt cccaacacga tggtgcgcga gcttaacgcg
     5881 gtccatccca acaccacttt ggccgatctg ccgccggaat tgcaggcagc gatcgccgct
     5941 aacgttcgcg ccgccttcac gcgtttgttg aaggaaggct attcggtgtc gcccggccgc
     6001 gcggctccgt cggccccgcc gccgcggacg cgggaagggc cgcggcccgg accgggcgct
     6061 cgaaggccgc cgcccaagcg ccccggcggc gatggcccga gatcgccgcg cgacggacgg
     6121 ccgccgcgcg acggcggacg gccgccgggc cgggggccga agccccgcgg ctagaccaag
     6181 cggccgccgc tagtgctccc ggccggccca cgctaaaggc gcgcggggac attgaggagc
     6241 gagagcgcat gaccgatcca tgggcggcgg tggaagcggc cgagacccgg ccgctggccg
     6301 atttgttcgc cgccgagccc gaccggctgt cgcgcctggt cgtcgaagaa gccggcatcc
     6361 gcttcgattt ctcgaagacg catcttgatg cgggccttgt cgccgccttc gcggcgctcg
     6421 ccgaggcgca cgacctggcc ggcgcccgcg acgccctgtt cgcggggcag gcggtcaacg
     6481 ttaccgaagg gcgctccgcc gagcatgtcg ccgagcgcgg ctcgggatcg ccggagagcg
     6541 tcgcccgcgc ccaggcgctc catgcgcgga tgcgggcttt gatcgacgcc atcgaggcgg
     6601 gcgcgttcgg tccggtcagc cacatcctcc acatcggcat cggcggctcg gcgctcggcc
     6661 ccgatttcct gatcgacgcg ctcggccgcg attccgggcg ctacgacagc gcggtggttt
     6721 ccaacgtcga cggcgcggcg ctcgaggcgg cgttcgggcg gttcgatccg gccgcgacca
     6781 tgctcgtgat cgcctccaag accttcacca ccagcgagac catgctcaac gcccgctcgg
     6841 ctttggcctg gctggaggaa ggcggcgtgg cggaccccta tggccgcgtc gtcgcgctca
     6901 ccgccagccc cgagcgcgcg atcgagttcg gcatcgacca gacccgcatc ctgcccttcg
     6961 ccgagagcgt cggcggccgc tattctttgt ggtcgtcgat cggcttcccg gccgcgctcg
     7021 cgctcggctg ggcggcgttc gaggagctgc tcgaaggcgc ggcggcgatg gaccgccatt
     7081 tccgcctcgc cgccccggcc gaaaacgcgc cactgctcgc cgccttcgtc gaccgttatt
     7141 acgccaatcg ccgcggcgcc cagacccgcg ccgtcttcgc ctatgacgag cggctgcgcc
     7201 tgctcccctc ctacctccag cagctcgaga tggaatcgaa cggcaagagc gtcaccgtgg
     7261 cgggcgatcc ggtcggccgc gccaccgctt cggtcacttg gggcggggtc ggcaccgacg
     7321 cccagcacgc cgtgttccag ctgctccacc agggcaccca tctcgtgccg gtcgaattcg
     7381 tcgccgcgat cgagcccgag cacggcctcg atttcgagca tcaccgccag ctgctggtca
     7441 attgcttcgc gcagggcgcc gccttgatgg ccggcaagcc ctccgacgat cccgcccgcg
     7501 cttacccagg cgaccgcccg tcgacgacga tcctgctcga ccgcctcgat ccggccaccc
     7561 tcggcgccct catcgccttt tacgagcatc gcaccttcgc caatgcggtg ctgctcggca
     7621 tcaacccgtt cgaccagttc ggggtcgagc tcggcaagga aatggccaag gcgatggacg
     7681 gcgacggcgc gatccgcttc gacccttcga ccgaagcgtt gatcgcgcgg gcgctggggg
     7741 aggggccgca atgatccccg gctcgaccaa ttttttcacc atatgctgcc gcgcccgctg
     7801 cggccggaag gcaaataatg acctacgact atgatttgtt cgtgatcggc gccggatcgg
     7861 gcggggttcg cgccgcccgc gtctccgccg cccatggcgc caaggtcgcc atcgccgagg
     7921 agcataagat cggcggcacc tgcgtgatcc gcggctgcgt gcccaagaag ctgctcgtct
     7981 acggcgccca tttcgccgag gatttgcagg atgcccgccg gttcggctgg gaagtgcccg
     8041 aatgcagctt caactggccg atcctgcgcg acaatgtgct tgccgaagtc agccgtatcg
     8101 agggcgccta tggtgagatg ctcgacaata gcggcgtcgc catattccat cagcgcgcgg
     8161 tggtcaccgg ccccaacggc atccgtctcg ccgacggcac cgagctcgcc gtccgcacca
     8221 tcctcatcgc caccggcgcg cggccgcagc tgcccgactt tcccggcgcc gagcatggca
     8281 tcacttcgaa cgaggtcttc cacctcgatg ccatccccgc ccgcgcggtc atcgccggcg
     8341 gcggctatat cgccaacgaa ttcgccggca tcttcaacga attcacctgc gacgtgactc
     8401 tggtcaaccg cagcgacgcc atcctgcgcc aatatgacgc gcaggtccgc gaccgcctgc
     8461 tccagatttc gctgatgaag ggcatcaact tcaagttcaa cgcgccgttc gagaaggtcg
     8521 acaagcagga ggacggaacg ctcgtcatcc agctcaaggg gcaggagccg atcgaggccg
     8581 acatgctgct gttcgcgatc ggccgcgcac ccaacactga ggggctaggg ctcgaaagcg
     8641 cgggagtcga gatcgacgaa tatcatgcgg tggtggtcga cgacgacaac aagaccgcct
     8701 gcgacagcat ctatgcggtc ggcgacgtca ccaaccgggt ccagctcact ccggtcgcga
     8761 tccgcgaggg ccaagtcttc gccgacaccg tgttcggcaa caagccgacg cgggtcgatt
     8821 atgccaacat cccgtcggcg gtgttcagcc acccgccgat cgccggcgtc ggcatgaccg
     8881 agtcggacgc ccgcaaccgg ctcggctcgg tcaagaccta tgtgtcggac ttccggccga
     8941 tgaagaacgt cctcgccggc cgcaacgagc gctcgctcta caaactggtc gtgaacggcg
     9001 agaccgatca ggtggtcggc atccacatga tcggccccga cgcgccggaa atcctccagg
     9061 ccgccgcgat cgcggtaaag gccggcctca agaaagagga tttcgaccgg gtcatcgcgc
     9121 tccacccgac catggccgag gagctggtgc tgatgcgctg accgtgttgg agggcgagcg
     9181 ggacgcggag gggatggatg agatcgtcca accccgtcat tccagcgaaa gctggaatct
     9241 cccttcactt tggggaaaga agggaaactt gcactgcaac cacggtgagt gatggccacg
     9301 aacgactata tgagcggctt cggcagccac ttcgccactg agtccgtgcc cggcgcgctg
     9361 ccgcaaggcc gcaactcgcc gcaaaggccc gccttcggcc tctatgccga gcagctttcg
     9421 ggcaccgcct ttaccgcgcc gcgccacgag aaccgccgct cctggctcta ccgcatgcgg
     9481 cccagcgccg agcacccgct ttatcgccgc tacgaaggcg cggagctgtt cgcgctgcgc
     9541 ccggccgtca ccggcgagct cgcgcccaac cggctgcgct ggagcccgct cgctttgccc
     9601 gaggcgccca ccgaccttat cgacgggctg gtgacgatgg tcgccaaccg cccgcccgag
     9661 gatctggagg gcgtcgccgt ccacatctac cgcgccaatc gcgacatgga gcgccgcttc
     9721 ttcggcaatg ccgacggcga gatgctgttc atcccgcagc tcggccggct aaccttgctg
     9781 accgagctcg gccggatcga cgtcgccccc ggcgaggtgg cgctcgtccc gcgcggcgtc
     9841 cgctttcgcg ccttgctccc ggacggcgag gggaggggct atgtcgccga aaatcacggc
     9901 agcctgttcc gcctgccgga cctcggcccg atcggcgcca acggcctcgc caacccgcgc
     9961 gacttcgaga ctccggtcgc ctggtatgag gatgtcgacg gcgaggtcga gctcgtccag
    10021 aaatatctcg gcgcgctctg gaccacgacg ctcgaccatt cgccgctcga cgtggtcgcc
    10081 tggcacggca atctcgcgcc ttggaaatat gatctcgagc gcttcaacac gatcggctcg
    10141 atcagcttcg accatcccga tccgtcgatc ttcacaatgc tcacctcgcc gagcgacgtg
    10201 cccggccgcg ccaatgccga tttcgtgatc ttcccgccgc gctggctggt cgccgaggac
    10261 acgttccggc cgccctggtt ccaccgcaac gtgatgagcg agtgcatggg cctcatccac
    10321 ggcgcctacg acgccaaggc cgaaggcttc gcccccggcg gcctcagcgc gcacaatatg
    10381 atggccggcc acggccccga cgtcgccagc tggaagggcg cgtccgaagc cgaattgaag
    10441 ccccacaaga tcgagggcac gatggccttc atggtcgaaa gctgctggcc gttccgcccg
    10501 tccgactatg cgctcggctg cggggagttg cagaaggatt atgacgaagc ctggggcggc
    10561 ttccccaagg cgcagctgcc accgcgctga tccaggagcg gcggggatcc aggagcgacg
    10621 gggactggcg cctctcagcg cgcgctgcgc ctcggttttc gtacgcgcgc ccagcgttcg
    10681 acccagccgc cgtgaaaaga cccccattcc caaacggcga tgacgataaa cagggcggtc
    10741 gccgtcaagt gcaggagcag gggctcgggg tgcgccacca gcgcccagag tccgtacagc
    10801 gcgaacaggg cggcgccgac gaggtgcgac agcggccagc ggcggatcgg gctggtcagc
    10861 cgcttgaacg ccatggtgcc gccgatgaac agcagagcgc cgcccagcag gcacgcaagc
    10921 aggaagggct cgctgtgccc ggtggggtgc atcaggacct gctcgtcggc aaccgcgagc
    10981 acgacgatgc ccgcgacgat cgggatatgg ccgtaagtat aggcggcgcg gccgatcagc
    11041 ccggggtgct cgtcatgctc catcagctcg gtgccgcgcc gcgcaccgat gtcgaaatag
    11101 atccaccaca tggcgaccga agcgacgaag gcgctgacga aggcgaggat ggtggcgcgc
    11161 gacggctcca attcgctgaa ggtcgcgccc gttaccaata tgccttcgcc gagcgagatg
    11221 atgatgaaca gcgcgcagcg ctcggccata tggccgccgc tgatctgcca atcctccgcc
    11281 cgcgagcggc cgaggccggg gaccgcgaag aacagcagcg gccccgcaca ttcgagcgcc
    11341 agcgcggccg cccaccaggc cgggcggcgg gaggggtccg gatccagccc gcccgcgatc
    11401 cacaaaggcg ccgaggcgac gaaatagagg gtcgcccgcg tcatgttctt ggaggcgccg
    11461 cgcacccatt cgcccttggc ccaggaggtg taggcggtac gcccgatctg gatcgccaca
    11521 taagcgagcg cgaaaggcag cccgtatggc ccgaacgccc cggggatcgc gctcgacatg
    11581 acgaggctta gcagcatcag cagggcgagc atcagccgca ccggcgaccg atccgggtcc
    11641 agccagttgg tcgcccacgc ggtgaaggcc caggcccacc agattgtgag gaacatcagc
    11701 agtgtctgtg cggcgccgat gaggctgtga tgctcgagca gataatgcga cagctgcgtc
    11761 accgcgaaca cgaagacgag atcgaagaac agctccatcg cactgacgcg atgaccgtcc
    11821 tggccggtgc cgccacgcag atgggaatgt cgaaccacgc tgcatcaagg cccaagtaga
    11881 gccgaggcgc aagacctcgc ttcagggccc gcccggattc ggcgcgccgc ttcgactttg
    11941 cggcctcgtg tcaaagatcg tctggcagct tcggcctctg cagctccgtt ccggttatgt
    12001 ttagtcggaa ggagaggccg gtggtttatg ccggcgccat ggcgccctca tcagatgatc
    12061 gggtgacgtt cccagttcac ccggtcggcg cgcttgcgca tcttgcggtg gagcttgtcc
    12121 atcgtccggc tgccggtctc gacgaacagg aagggcagga tagcgtggac cagcacgcac
    12181 aggccgccca ccatcatcgt cgcgccgaag ccggcggccg tgccgagatg ctcgccgtag
    12241 ctctcgccga tttccttggg gtgccgcgtg aaaggattgc ccgccattgc ccctcgcctt
    12301 ttctttgtct ggtcggagcc aggctccgcc gcacccccta gccgctgccg ccccgatccg
    12361 ccagaggaag gcgcgccatg ttcgaccgat tccccgaagc cgccggcgat gccctccacg
    12421 cggtaatggc gcgcttccgc gccgacccgc ggccgcacaa gatcgacctc ggcgtcggcg
    12481 tctatcgcga cgccgacggc gcggcgccgg tgatgccggc cgtcaaggcg gccgaggccg
    12541 tcctgctccg cgacgaggac agcaaggtct atcaggcgct gcacggcgac gaccgcttca
    12601 tcaccgccat gaccgacctg gtgttcgggg cggaccatcc cgcggtccgg gacggccgca
    12661 tcgccgccat ccaggcgacc ggcggcaccg gcgcgctccg gatcgcgttc gacgccgccg
    12721 cgatcgccgc tccgggcgcg aggctgcacc tcggcgagcc gagctggccc aaccacgccg
    12781 cgctcgccgc cgccgccggg cttgcgctcg cgccgcattc ctatttcgac gttgccgcgc
    12841 aggcgatcga cctcgccgcc atcggcgcgg ccgcgggcgg cgccggcgag ggcgacctgt
    12901 tcatcctcca cggcccctgc cacaatccga ccggcgccga cctcaccctg gagcagcggc
    12961 gcgatctgct gtccgtgctc gccgggcgcg gcgcggtgcc ggtcatcgac gcggcttatt
    13021 acggcctcgg cgacgggctc gacgccgacc tccgcatcct gcgcgaggtt gccgccgcct
    13081 tgccgcgcgt gtttatcgcc atctcctgct ccaaagtgtt cggcctctac cgcgagcgca
    13141 ccggcatcct gttcgcgctc tgcgccgacc cggccgaacg cgcccgcgtc cagggccagc
    13201 tcgaagggat cggccgcact ttggtgtcga tgccgccggc ccacggcgcc ggcacggtag
    13261 ccatcatcct cggcgacgaa gcgctgcgga aattatggcg cggggagctc gaccggatgc
    13321 gcgcgaggat cggcgggctt cgcgccgagc tcgccgcttt ggccaatgtc gtgccgatgc
    13381 tgcgtgggat cgaggctcag cgcggcatct tccggatgct gccgctgacg cgcgagcaga
    13441 ccgagcggct cggccgcgat catgccatcc acatgccgcc gtcggggcgg atcaacctgg
    13501 ccggcctcaa gcgcggggac gccgcgcggc tggccgaggc gctggccgcg atcgaccccg
    13561 cctgagcggg atcagccgcg gcagacctgc accggaacat cctcgcgctc gccgcggccg
    13621 cggacccatt cggtttcgca ccgcatggtc cgcggcggtt ccgactcggc ctcggcctcc
    13681 gcgccgtaat tgaaccagtc atagggatag ccacggtcgt aatcgaaatg gcggccgttg
    13741 gtggcgccat gatcgaaata gccgttgtgc cgcgacaggc gatcgtggcg gaagagcggc
    13801 gtgtcgctga gctgcagata ttgcccgccc ggctccgacg ctatccagcg cgcctcctcg
    13861 gggtcatagc cgtcgaagcc ttgcgccgcc gccgtcccgc ccgccgccgc aagcgccagc
    13921 gccgccgccg cgatccgcat cctcttcatc gccacctcca gctgcatcga gtcccgcgcg
    13981 ccacgctagc agatcgcgct gggccgcgcc gcccccgggg cgagatggcg cgtttcagaa
    14041 ggggacggct cagccctccc gcttcatctt ctcccgccgc gcaagctcgg cctcgctcgg
    14101 ctcgtccggc acgaacgatc cgacttggac cgggccgtcg cgctcatagc tggcgatgat
    14161 gacgccggtg ctcgagacct tgctctcgac cagcttcagc gcctgcggcg gcagcccgtc
    14221 cgcgaacagc cttttgcctt tgccgaggat caccggatag gtcatcagga acaggcggtc
    14281 gaccaggccg cgcccgagca gcccggcata gagtttgctg ctgccccaga tgcgaagctc
    14341 cggcccgtcc tgttccttga gcctcgccac tccatcaatg ccgtcgctca ccgcatgcga
    14401 gttggaccag gcgagcggct cggccgagga ggtgacgaca tgtttcgccg cccggttgaa
    14461 caccgtccca atcggatcgt cccggtaggg ccagtgcgcg gcgaagatgt cataggtgtt
    14521 gcggccgagc agcaggtcga acggctcggc gaagagcggc gccatgctct cgccgatcgt
    14581 gtcgtcggac agcggcgcgc tccagccgcc gagctcgaag ccgccggtcg ggtcctcctc
    14641 cggcccgccc ggcgcctgca tgaccccgtc gagcgagacg aaggcggcgc cgattatgtc
    14701 acgcatcggc ggcctcgccc tcgaaggcgc gttccagcgc ggcgatgtcg agccgcttca
    14761 tctccatcat cgcgtccatc gcccgcttgg ccttggcgcg gtcggggtcg gccagcatcg
    14821 ccttcatcac gcgcggcacg atctgccagg aaagtccgta acggtccttc acccagccgc
    14881 attgctcggc ctccggcacc gccgaaagcg cctcggtcag ccggtcgacc tcggcttggt
    14941 cgtcgcagtc gatctggaac gacaccgcct cgttgaactg gaaatagggg ccgccgttga
    15001 ggccggtgaa cttcgcgccg gccagcgtga attcgaccac cagcacgttg ccggcctggc
    15061 ccgacggata atcggacggc gcccggacga cattgtcgac gcggctgtcg gggagcagcg
    15121 acacgtagaa gttcgccgct tcctcggcat tgccatcgaa ccacaggcag ggggtgagct
    15181 tggacatggg gcatcctttc cgaaaatcgg tttcttggtc agtggtcagg cgcttcccga
    15241 agggagggga gggcgatcag gcgagctcgc gctcggccgc cgccgcgccg gcgccggccc
    15301 gttcgtcgag caccacgtcg aagccgcccc agaacatgcg cttgccgtcg aacggcatgt
    15361 cgtcatggtt cggctgcatt tcgggatccg ccatcatctt tgcccagccc gcgtcgcggg
    15421 tcgccttgtc gggccattcg atgaaggaga agacgacatt ctcgccttcg cccgcctgca
    15481 ccgcacggcg gaagtcggtc tgcttgccgt ccggaatgtc gtcgccccac gcttcgacca
    15541 ggcgcagcgc gccatgtttc tcgaaatcgc cggccgcttt ccgcgccagc gcgagatagg
    15601 cgtccttctt gtccggcatc accggcagca cgaacccgtc gacatagggg ccgcggcccg
    15661 atccctcgtc gagcaagccg gcaaagccgc cgaagatcat gcgcttgccg tcgaacggca
    15721 tggtcccgcc gatctccttc attctggggt cggtcatcat cttctggccg accgcgtcgc
    15781 gggtcgtccg gtcgggatat tcgaaccagg agaagacgac gtcctcatcc acggtcgcct
    15841 gcacggcctt gcggaagtcg gtcagcttgc cgtccggaac gtcgctggcc caggcttcga
    15901 catggcgggt gacgccgaac tccaggaaca aaggcgccgc gccggctgcg tggcggatat
    15961 attcctcctt attcgccttc ggcaccgcga cgacgaaccc ttcgacatag atcatggcgt
    16021 ctctccctct tatggttttt tcaggccgcg atcgccgtgc ccgcctccgc ttcggccagc
    16081 tcggcgagct gcgccgcgac cagggtccag ccttgctcga agcccatctc ccgatgcttt
    16141 tccaccgcct ccggatccca atgctgcacg cgcgccgtgt agcgcgtgcc cgacccttcg
    16201 ggctcgaagg tgacgatcgc caccatcgca aagtcgcagc cctcgttggt ctgcacctgc
    16261 ggctgccagc cgggcttgaa ggcgttggtc cagacgatgc gttggttcgg aaccacctcc
    16321 aggaacacgc cctcgccgcc ttcgccctcg ccgtcggggc cgcgcatttc ggtatacatg
    16381 cgcccgcccg tgcgaagctc ccattcgacg gcgggcgtgg tccaaggctt gggcacccac
    16441 cattccgccg tccgctcggt atagacgcga tagacggtcg cgggcggcgc atcgatcaag
    16501 cgggtgaccg aaagttcgag tgtgtcgctc atgggattct cctcctgatc ctcttttagc
    16561 cgcgcttgcc gaccagcgcg aacagggcgc cctgcgggtc gcggcactgg acgacccagg
    16621 cattgccagg cacctccatc ggcccttcga gcagctcgcc gcccgccgcg cgcacccgct
    16681 ccgcggcctc gtcgatgtcg ccgacggcga aataataaag ccagccgggc cggccgaacg
    16741 tgtcggcatt gtacatcgcg ccgatccagt tcggcccgtc cgcgctgacg atctggtaga
    16801 tgcccatcgg gcccatatcc atcgcctcgg ccttggccca tccgaaccgg ctcgaataga
    16861 aatcgaacgc cgcctcccag tcggtggtgt gaagctcgtg ccaggcgaca tggccgtccg
    16921 acatgggcgg gaccggcgcc tgcgcttcgc cctcgggcgt gatcaggtag aagggcgccc
    16981 cctgcgggtc ggcgaccatc gcgatccggc cgaccgtcgg cacgtcgatg atgtccttgt
    17041 ggagcttgcc gcccgcctcg acgatccgcg ccgcttcggc atcgacgtcg tcgaccgcga
    17101 tatagccgaa ccagaccggc cgggcgccgg tctgccgcgc atcttcggga attggcatca
    17161 gcccgccgac gccgcggccg ttcgcctcca ggatggtata atcgcctccg ccctcgaagg
    17221 ccttggtctc ccagccgacc acggcgcggt agaagccctt ggccgcctcg gtgtcgctgg
    17281 tcatcagctc gtaccagatg aagctcgccg ccatgtctcg tctcctctgc cccgggggcg
    17341 cttcaggcgt gttcgaaggc ggcggcgccc tgctcggcgg cggccgggtc catccacatc
    17401 acttcccaga tgtggccgtc cggatcctcg aagctgcggc cgtacatgct tccataatcc
    17461 tgggtcggcg tcgggtcgag gacgcccccg gccgcgcgcg cggtctcgat ctgcgcgtcg
    17521 accgcctcgc ggctgtcggc ggaaatgcag agcagcattt gcgcgttctt atgcgcgtcg
    17581 gggatctgct tggtcgtgaa ggtgcggtag aaatcgtggg tgagcagcat cacgttcacc
    17641 gcttccgaaa agcgcatcat cgccgcggcc gcgttggtga acttgggctc gttggccgcg
    17701 cccaccgctt catagaagcg catcgagcgc tccaggtccg tcaccggcag gttcacgaaa
    17761 atcatgcggc tcatctttca ctccttgatg cgaatcgctg tttaccgaat cgctgtttga
    17821 cggtgatgat ggcttgagtt ataaaaagca actatgaagt tacaaaacgt aactgagtcg
    17881 cgacgggccg ttgccaagcg ccgatatgac gacgcctgcg gcaccgcgca cgcgctcgat
    17941 ctgatcgggg agcgctgggc tttgctggtg atgcgcgagc tgatgctggg cccgaaaagg
    18001 ttcagcgaca tccgcgccga cctgccgggg atcagcgcca acgtcctcac ccagcgcctc
    18061 gaaggtttgg aggcggccgg gctgctggtg cgccgccggc tgccgccgcc cgccaacgcc
    18121 caggtctatg agctcacccc ctggggctat gaggccgagc cgatcgtcca gtcgctcggc
    18181 cgctgggcgg cccgctcgcc ggcgcacgat ccgacgctgc cgatcagccc ggtgtcgctg
    18241 atgctgagct tccgcaccat gttcgatgcg gagcgggcga gggggctcga cgcgcgaatc
    18301 ggcttccggc tcggcgccga gagcttcctg gcgcgaatcg ccgacggcaa gctcgaggtc
    18361 gagcgcgccg agcccgcttc ggcggacgca atcttcaccg gcaccagcgc cgccctcgcc
    18421 gccgccgtct atggcggcca gccgctgccg gccctcgccg aagccggcct gctcgccgcc
    18481 accggcgacc aagccgtcgt gcaacgcttc ctaacgctct tcccgttgcc gccgaaggta
    18541 agcgaggccg ccccgcccgc ctgagtcgcg cctgcgcacc tccccgtcat gccggacttg
    18601 cctgccctga cgaaagtcag ggatccggca tctcacttca tctttcgccc cagcgacgca
    18661 agacaggaga cccccgatcc ctgacatttc gtcaggacag gcaagtcggg gggtggcgat
    18721 tcgcgcgagc gacttggcgc tggctccaga cgcttatgtc agggcaggca agtccaggtg
    18781 acgcttcgga cgaatgaaaa atcgggcgcg cgaccacgcg ctggcggcag gcgtcgaact
    18841 gctcagccgc ctgacccgca gtcatcggag gacgctgcgg cgcagccaaa aggccgcagc
    18901 aaaacccgca atcattccga ccacgcagcg gctcagccag aaggacgtag caaaacccgc
    18961 aaccatccta gggcgaagcg gcgcagctaa acgggcgcag caaaaacgga gaactcgcca
    19021 cggagagtgg aggaggcagc aaaaagggag acccgcggcc acggcttgcg ccgggacccg
    19081 gaatctccct tgttgcggca tacgccgcag cgcggcggcc gttcagccgc ccgcgttcag
    19141 gctcccgatc ctatccgccg atcggcggcc gtggatcggg caagcggtcc ggagcaaatc
    19201 ctcgccgggc cgcgcgaagc ggcccggagc ggaatgctta gccgcgctcg ccgcgacgaa
    19261 ccggcggctc gggcggagcg aaggtcgccg tggacatcgc cgtccagcgc gaggtgccgc
    19321 aatcgctggt cagcgacatc gcctggccgg gctggaaggc ggcctgatag gcccagcatt
    19381 cccgcgcggt ggtgccggtg gcgaggcaca ggcgctggtt ctcgacgaac cattgcgccg
    19441 gaacttcgcg gcccgccggc gagacgatgc gggcgctgcc gccggcatcg aaatagacgg
    19501 tgttgacgac gccgttggtt tcgacctgaa cggaatgccc ggtgatctcg gctccggtgg
    19561 gcgggcccat cccttgggcg gaggcgcctt gcgccccgaa cgttgcagcg agaatgaagc
    19621 ctcccatggc aacaactgac tttttgatca tcatgcattc ctcccgtgat ccgacggagc
    19681 aaacgacccc gccatgaggt ggttccggcg caaaaacgtt tttaggcttt tggctcgcgg
    19741 aagtccaatc cgattctcta tactggtcct gccgcatccg cgggcggccc ggccggcgcc
    19801 tcagggagtg cgtgcaaatc cccgggaagg cggctttttg ggaagaatga tgcgggcgcg
    19861 gaacggcttg ggcgccagcg cgttccgcag tttccaatgt ccattcgcat cggcttaggt
    19921 atgtcttcca taccgctgcg aacactgcta tggggacttg gcagaaaggg gagtttctct
    19981 atgctttttt caaagccgtc ggtaatcaca aaagccctta tgggcgcagc gctctgcttg
    20041 agctcgactt ccgtatacgc gagctccgcg gcggcgtgcc aggccggaca ggccggatgc
    20101 gtgttgccgg taacggatcc cgcagcggcg cctgcgccgg cgatggaaac gtcggcgccg
    20161 atcatcgagg aaggcacggg catcgggatc cttccgatca tcgccgtgct cgcgctcgcg
    20221 ggtctcgcgc tcatccttct cctcgatgac gacgaagaag agaacagccc gatctgatta
    20281 gggcgttcct ttctgaaaac gtggagattc ggcggtaacg ctggcctccc ttggagtaca
    20341 aaaagcctcg gccttgcggc cggggctttt tgctgtgcgg gaccggcgaa gtccccgcgg
    20401 acgcggagcg cgcgccgcgc cgatggctca ggctccggcg ccaggcgagg actggagcag
    20461 ggctgacctg tggttcggcg ccatccgcga atcgcgcggg gaagctgttg ccggcgccca
    20521 acacgcgccg ctgtcacccc ccagtcgcag cccgagcgcc accctcacgc ccgcggcccg
    20581 cacccgccgc cgctcgccca aaagtccgat cgcgtcgatt cggcgcctgc ccctgaccca
    20641 ggtgagggat tcggactctc ccttcttcaa gcccgatgcg gaagaacaaa ataagggggg
    20701 agggggccgg tccgcacccg accggcggcg gtcatgacgt gaatcgtcga tccggccgcg
    20761 ggcttcgccc ggtctgggcc cctgccttcg caggggcacc aagaagggca aacccaagcc
    20821 tgcgcgacgt gccgcaacat cgcatctacg ccatcagaag cgggagcatc gtgcccctgc
    20881 gaaggcaggg gcccagaccc gggctgacgc ttcagcggcg gcccggtcat cgcagcggct
    20941 gtggaagcct ggcgccgtag cgcgctaggg tcccggactc gcggatatcc acgccagctt
    21001 agccggcggg ctttcgcccg gcctggtccc ctgccttcgc aggggcacca agaaggcaaa
    21061 gcccaaacct gcgcgacgtg ccgctacatc gcatccacgc catcagaagc gagcatcgtg
    21121 cccctgcgaa ggcaggggcc caggcccggg ttgccgcttt accggcagcc cggtcatcgc
    21181 agcggctgtg gaagcctggc gcggtagcac acaggggccc gggctcgcgc cggacccgac
    21241 tccgggggct cggacgctgc cgccccggcc tggtcccggg ccggcatcac gcggagtctt
    21301 ggatcgcgcc gcaccgcctg gccggcggca gccgctcagt cggatcccgc catggcgctc
    21361 gcgaagcggc ggttgcggtc ggagtcgcgg ctataggggt cgtcgcgcag cgccagccgc
    21421 tcgccgtccc aatccaccgt cagataggtg atgtagaccg gcaccggctc gggcagc
//
LOCUS       DASVRK010000144        29758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_587865, whole genome shotgun sequence.
ACCESSION   DASVRK010000144 DASVRK010000000
VERSION     DASVRK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 29758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29758
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587865"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="hemE"
                     /locus_tag="VF589_05690"
     CDS             <1..1018
                     /gene="hemE"
                     /locus_tag="VF589_05690"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX8257103.1"
                     /translation="QTKPLLAVLKGGKADRRPVWLMRQAGRYLPEYRELRAEKGGFLA
                     LVYDSDAAAEITLQPIRRFGFDGAILFSDILIVPHALGQDLKFEAGEGPRLSPPLVDA
                     ALRALEAVPARLNPIFRTVDKVKAALPAEVTFLGFAGSPWTVATYMIAGQGSKEQAEA
                     RRYAYRDPQAFAAIIDAIAEMTVDYLAGQIEAGVEAVQLFDSWSGSLAPAQFEQWVIA
                     PTARIVAGLKQRHPHVPIIGFPKGAGGKLAAYARETGVDAIGLDETVDPEWAAAALPK
                     QLPVQGNLDPLALIAGGEALEKAVQRIISAFEDRPHVFNLGHGILPDTPIAHVEQLLR
                     LVRQ"
     gene            1048..1473
                     /locus_tag="VF589_05695"
     CDS             1048..1473
                     /locus_tag="VF589_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX8257104.1"
                     /translation="MTYAWIKAAHVIFVIFWIAGLFMLPRFYVYHQEATPGSVEEQRW
                     LDRERKLRNIIITPAMLLVWVFGLTLAYITGAWAEGWFHAKFALVLLLSGYHGYMVGY
                     GKKLARGQRPASGRSLRIMNEIPGIATALIVILVIVRPF"
     gene            1607..2863
                     /gene="rho"
                     /locus_tag="VF589_05700"
     CDS             1607..2863
                     /gene="rho"
                     /locus_tag="VF589_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX8257105.1"
                     /translation="MHLKQLKQKTPADLVAMAEELGIEGASTLRKQELMFSILKVQAE
                     NGQEIMGMGTIEVLPDGFGFLRSPEANYLAGPDDIYISPNQVRKFGLRTGDTVEGEIR
                     GPKDGERYFALTRLISVNYDDPDAVRHRVNFDNLTPLYPDEKLTLDTKDPTVKDKSAR
                     VIDIVSPQGKGQRALIVAPPRVGKTMLLQNIAKAITDNHPEVFLIVLLIDERPEEVTD
                     MQRSVKGEVISSTFDEPAQRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSKMDEVIFEE
                     FKGTGNSEIVLDRKVSDKRIFPALDVGKSGTRKEELLVDTAKLSKMWVLRRILMQMGT
                     VDAMQFLLDKMKDAKSNEDFFASMNQ"
     gene            2888..3604
                     /locus_tag="VF589_05705"
     CDS             2888..3604
                     /locus_tag="VF589_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX8257106.1"
                     /translation="MMELFFNLQAAVPMGIGGIWDAIVLDFSKLNTAAAWVVFGQVLM
                     IDIMLAGDNAIVVGALAAGLPPEQRRKVILIGIIAALVLRIAFALVVTQLMQIVGLTF
                     AGGLLLLWVAWKMWRELQHSGQSQGSPEIRGDEDSGLKPAKSFAGAAWAVAVADVSMS
                     LDNVLAVAGAAREHPGILIIGLLLAVALMGLAANILAKYIERYRWIAWVGLLVIVYVA
                     GKMVYEGFIDPQVGLGTLFA"
     gene            3713..4696
                     /locus_tag="VF589_05710"
     CDS             3713..4696
                     /locus_tag="VF589_05710"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX8257107.1"
                     /translation="MGRHLLPFGFAAAALTASLSAAEPPAAPTRAGAERDVIAFEQDT
                     AARMTVPVSIGGRGPYRFIVDTGSERTVISEELARNLELSAGREATLHSISGAGEVST
                     VVIPNLRVSKRNVRGIHAPALAAADLGAVGMLGVDSLKSQRVLFDFGRQTMAVTPSVQ
                     YEQDWGPNTIVIKARTRYGRLVLSNARIEGEKVLVILDTGSEVTIGNEALRRKLAARG
                     KLRSTRPISLLSVTGGLAPADYTTISHVRLGGAYIKQLPVAFADIHPFRQLDLLDRPA
                     LLLGMDALQLFDRVSVDFAHRRVQFLAPDLGLLEEQTRMAALAGGSRTASR"
     gene            complement(4698..5678)
                     /locus_tag="VF589_05715"
     CDS             complement(4698..5678)
                     /locus_tag="VF589_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX8257108.1"
                     /translation="MGPPYSFTLRRTGGPEILEPEPMELTAPAEGEILVRHEAVGLNF
                     IDTYYRSGLYPLTLPSGLGSEAAGLVEAVGPEVSGFREGDRVGYFSGPLGAYATHRTI
                     AADRLVRLPTGIAPEIAAAAMLKGCTAEFLIERCARVQAAQTVLVHAAAGGVGSILVQ
                     WLKALGATVIAHAGSEAKAALAAEAGADHALDCPMGALADAVRERTGGKGADIVLDGV
                     GAASWAASLGSLARRGLLVTFGNASGPVPPFRALDLTKAGSVFVTRPTLADYIAAPGE
                     MEASAARLFEMIGNGSVRPGIGATYKLSDAAAAHRALEARATTGSTILVP"
     gene            complement(5710..6408)
                     /locus_tag="VF589_05720"
     CDS             complement(5710..6408)
                     /locus_tag="VF589_05720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8257109.1"
                     /translation="MGMIDIETLEQDGSFKAYLAEPEGPPRAAIVVIQEIFGVNAGIR
                     RKCDHWARLGYLALAPDLFWRLEPGVELDPDVEQEFKQALDLMGKFNQDQGVRDIEAT
                     LGAARKRLNKNGKVGCVGYCLGGRLAFMTSIRTDVDATVGYYGVGLDSLLGDKEAIAR
                     PLMLQIAGEDGFVPKDVQARIHEALDGHPKVVLHDYPGEDHGFAAEMGKRRSEDSARL
                     ADERTEAFFAEHLG"
     gene            6495..6728
                     /locus_tag="VF589_05725"
     CDS             6495..6728
                     /locus_tag="VF589_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HEX8257110.1"
                     /translation="MKVTVDVDCTPEEARRFIGLPDLSPVHQAYVEKMQKAVTDGLTP
                     DAVGEMMKAWGPMSDAGMAMWRQMLERMGGTKA"
     gene            6753..8036
                     /gene="mnmE"
                     /locus_tag="VF589_05730"
     CDS             6753..8036
                     /gene="mnmE"
                     /locus_tag="VF589_05730"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX8257111.1"
                     /translation="MAADTIYALSSGSPPAGIAVVRISGPGADAALMALAGRLPEPRR
                     AGVAALRHPLSGELLDRALVLRLPGPGSATGEDVAELHLHGGRSVVAAVLGALAAVPG
                     LRQAEPGEFTRRAFEIGRIDLAEAEGLADLLAAETEAQRRAALQLAGGALSREVERWQ
                     AELLGLAAELEAALDFADEGDVGADLPAGWRERLARLCGELKAALARPPAERLRDGVR
                     VVLAGPPNAGKSSLFNALAGREAAIISPLPGTTRDLIEAPVAIAGMPFLLVDTAGLRE
                     SGDAIEAIGVDRARSSLAAADIILWLGNPGAAPDRDRTLVVGAKADLGPPPPGTDLPV
                     SAITGEGIERLVDCLRERAKSLLPGEGEMAINARHRGILRDVLASLGEAGDTDDLLIA
                     AESVRLGRLALDRLTGRAGVEDMLDALFGRFCIGK"
     gene            8104..9957
                     /gene="mnmG"
                     /locus_tag="VF589_05735"
     CDS             8104..9957
                     /gene="mnmG"
                     /locus_tag="VF589_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX8257112.1"
                     /translation="MTRFDIIVIGGGHAGCEAAAAAARMGASVALVNFNRDTIGAMSC
                     NPAIGGLGKGHLVREVDACDGLIARAADHAAIHYRMLNASKGAAVRGPRVQADRRRYR
                     SAIHDLLAGQSGLTIVEGEASALRLEGRRVTGLTLADGRELAATAVVLATGTFLGAKL
                     HFGMTSCAGGRVGERAATELAGQLRGLGLPLARLKTGTPPRLDGRTIDWSRLERQPSD
                     DGGWTLSAMTEARAPPQLFCAITRTNSRTHEIIRASLDRSPLFGGEITGVGPRYCPSI
                     EDKVHRFADRDGHQVFLEPEGLDDPTIYPNGVSTSLPADVQLAMVRSMEGLERAEILQ
                     PGYAVEYDHIDPRILDPTLAVRPELAQGLYLAGQVNGTTGYEEAAAQGLVAGVNAAAW
                     VAGKPPMLFDRSESYIGVMVDDLTLQGVTEPYRMLTARAEYRLRLRADNADVRITPQA
                     IAADCVSHQRRKMFVGMQQGRADIEELLGQRFTAKQLLAAGAEVRDEGVSRTLAEWTR
                     FPNVGADALVRIAPALAAADRKLLDSAVEDHRYAPYLDRQQAEVARLKADEAVRIPAH
                     FDYGGVPGLSNEMVERLSSARPHSLGAARRVRGITPAALASILVHLRRKAA"
     gene            9957..10598
                     /gene="rsmG"
                     /locus_tag="VF589_05740"
     CDS             9957..10598
                     /gene="rsmG"
                     /locus_tag="VF589_05740"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX8257113.1"
                     /translation="MDEDEARQRLIGEFGVPRETLARLKLFVNYLAAENERQNLVSRA
                     SLDEVWSRHILDSAQLLSFAPRSVGSWLDLGTGAGFPGLIVAALRRDIAVTMVEARRL
                     RVAFLEAAADILRLRPDTIIRCAKVEQVESAPFDVISARAFAPLDRLLALGERFATAN
                     TRWILPKGKNAKTELDAAESLWQGSYRIEPSLTDADAGIIVAENVRRKARARR"
     gene            10595..11377
                     /locus_tag="VF589_05745"
     CDS             10595..11377
                     /locus_tag="VF589_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8257114.1"
                     /translation="MIRIAVANQKGGVGKTTTAINLATALAATGWKVLLVDLDPQGNA
                     STGLGVAHHERNQSSYELLTGGCTLEEAVVATRIPRLDLVPSTVDLSGAEIELVEYEE
                     RTHRLDKSLNSASGGRWDICLVDCPPSLGLLTINALVAARAILVPLQAEFFALEGLSQ
                     LLQTVERVRARFNPELSILGVALTMYDRRNNLSAQVAADVRACLGKVVFDTVIPRNVR
                     LSEAPSHGVPALIYDHRCSGSEAYMSLARELIGRLPAPAAAA"
     gene            11374..12258
                     /locus_tag="VF589_05750"
     CDS             11374..12258
                     /locus_tag="VF589_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX8257115.1"
                     /translation="MIDETPIRRRAPGLGRGLSSLLGEVAQEAPIAGGSGRGGIQMLP
                     VASIEPNPNQPRRSFDEDALTELAESIRTRGLIQPIVVRPREGGYQIVAGERRWRAAQ
                     RARLHDVPVIVRDFDDVETLEVAIIENIQRQDLNAIEEAEAYKRLLDEFGHTQEALGR
                     LVHKSRSHVANLLRLLDLPSKVRAWVASGELSMGHARALLGTSDPEACALEVINRDLS
                     VRETEKLARADKPRRPGPIEHKGASADIEALERQLGDMLGLKVRITHRPDGGQVTLNY
                     SSLDQLDMICQRLSGERI"
     gene            complement(12275..13300)
                     /gene="holA"
                     /locus_tag="VF589_05755"
     CDS             complement(12275..13300)
                     /gene="holA"
                     /locus_tag="VF589_05755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX8257116.1"
                     /translation="MKASRGQIEQMLRAPSPDIRWTLLYGPDEAGSRALALSLAKAMG
                     AGAERIDLSGADLKSDPARLADEAASISLFGDPRYIWVEPAGDEALPAVEALLEAPAA
                     GNPVVLIAGNLKPTSKLLKAALAAPSAMAFASYLPDAREAARLVTELGREQGLDMRSD
                     VARRIADAAGGNRALIGQELAKYALYLDAAPDRPKPLDEEAVEAVGAANEEGDLSRLV
                     EAVAGGDLGLLQTEMARLKSEGIEGISLIRAVVRRMTLLAKLRSEADRGNSVAAVMAS
                     HGKAVFWKEKDAVASQLSRWRSDLLAKSMGRLLEAERQVKASGGVGPVAVDEELFAIC
                     RQAGRLR"
     gene            complement(13297..13791)
                     /gene="lptE"
                     /locus_tag="VF589_05760"
     CDS             complement(13297..13791)
                     /gene="lptE"
                     /locus_tag="VF589_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX8257117.1"
                     /translation="MIRAALLLTGLLLSGCGLRPLYSGGERGPVAQTLRSITVAPIAG
                     RSGWLVRTALEDRLRAPEGAAPRYRLEVELDDDITGFGIRNNAVTRERRTLRARYRLV
                     DASLGTVLLDATAGSDAGIDVVGSEYATVAAEQTALERLSTQVADQIVARVALYVSRT
                     GGGR"
     gene            complement(13788..16307)
                     /gene="leuS"
                     /locus_tag="VF589_05765"
     CDS             complement(13788..16307)
                     /gene="leuS"
                     /locus_tag="VF589_05765"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX8257118.1"
                     /translation="MASRFNPRTADARWQQVWEERGSFRASDDSGKPKSYVLEMFPYP
                     SGRIHIGHVRNYTMGDVLARYRRMKGFEVLHPMGWDAFGMPAENAAMEKKVHPGEWTR
                     ANIATMRGQLKRLGFALDWSREFATCEPDYYGHEQALFLDLFEAGLVYRKQSEVNWDP
                     VDMTVLANEQVIDGCGWRSGALVERRKLNQWFLKITDFAEDLLEGLGALDQWPDKVRL
                     MQENWIGKSVGMRFRFALERPAAGMDSLEVFTTRPDTIFGASFAAISPDHPIALELAG
                     RNEAAATFIAECKRGGTTAAELETAEKKGHDTGLAVIHPLDPEWKLPLYIANFVLMDY
                     GTGAIFGVPGHDQRDFEFASKYGLPIRRVVAPAAQQADDPVEGEAFTGSGVIVNSRFL
                     DDLEVEPAKAEVIRRAEADGWGEGTVVWRLRDWGVSRQRYWGTPIPIIHCEACGAVPV
                     PKDQLPVVLPEDVSFELPGNPLDRHQSWSHVDCPKCGAAARRETDTLDTFVDSSWYFI
                     RFASQPGDRPFDASVAESWLPVDQYIGGVEHAILHLLYARFWTRALKRIGRIAVDEPF
                     KGLFTQGMVTHETYKAGDGSWLSPHEVRRDGDDWVHIESGQPVTAGRVEKMSKSRKNT
                     VDPDTIVDQYGADAVRWFMLSDSPPERDLPWSEAGIEGSWRFVQRLWRIGSNAETGMG
                     EDRALDRKLHQTIAAVAANIEALAFNKAVANLYELANAIEKAPPSASRARAAETILLL
                     VAPMVPHLAEEAWSAAGREGLIADQAWPTVDPTLLAEDEVTIAVQVNGKLRDTLTAPK
                     GAARETLEEMALASEKVVRILEGKPPRKVIVVPDRLVNLVA"
     gene            complement(16321..16752)
                     /locus_tag="VF589_05770"
     CDS             complement(16321..16752)
                     /locus_tag="VF589_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEX8257119.1"
                     /translation="MNRAVSLGLAIACAVTLGACSRNRALPQADLAASQVTTIGVNSY
                     LWRAALDTLSYAPLAQVDSNGGVIVTDWYANPASPGERVKVTATILDRDLRADALRVA
                     ASRQVMQNGQWVEAPVAAATVQKLEEIILTKARDLRRAAVG"
     gene            complement(16863..17168)
                     /locus_tag="VF589_05775"
     CDS             complement(16863..17168)
                     /locus_tag="VF589_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257120.1"
                     /translation="MGPAFYIMAIMGCGEADTACQQVAVAPARYESVEACNAATEDAL
                     MNNSDVAFPVVVAQCKPAAGAISQTLMPEQVDLPEPRPGQQQRIDRATVKPQQLAKL"
     gene            17280..19100
                     /locus_tag="VF589_05780"
     CDS             17280..19100
                     /locus_tag="VF589_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX8257121.1"
                     /translation="MPPETTNPTERPEAGWQALRRFLPYLWPAGEPALRARVVAALAL
                     VLIAKGATLIMPFAYKAAIDQMAPGMEAAAGLAMAFVAAYAGARFGGVLFDNLRNAIF
                     ERVGQDAARRLAEHVFRHVHKLSLRFHLERRTGALTKIVERGTKSIDMMLYFLLFNIA
                     PTIIELIAICVIFGVKFGPGLVVATLAMVALYIFFTRLVTDWRSQLRRELNDIDNKAI
                     ARAVDSLLNYETVKYFTAEEREARRYGKAMRAYQESAIRNETSLAWLNIGQSLITNVM
                     MAGAMAYTVWGWSRGQFTTGDVVLVNTLLAQLFRPLDMLGWVYRNIRQGLIDMEQMFD
                     IIDTQAEVKDKQGAPELRVGAGHVRLEGVRFGYEPDREILKGIDLDVPPGTMLAVVGP
                     SGAGKSTLSRLLFRFYEPTGGRITIDGQDICEVNQASLRAAIGIVPQDSVLFNDSIGY
                     NIGYGRDGAGQAEIEAAARGAAIHDFIMGLPAGYDSQVGERGLKLSGGEKQRVAIART
                     LLKDPPILILDEATSALDSRTEAAIQETLRSVAARRTAIVIAHRLSTVVDADQIVVLD
                     EGRVAERGTHAQLLRKGGLYAEMWARQQSEREQDAAEAAE"
     gene            19395..20405
                     /locus_tag="VF589_05785"
     CDS             19395..20405
                     /locus_tag="VF589_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8257122.1"
                     /translation="MTLRQTIQSAPAKAGAIIEKLSNTSNQAIKTRESLFAELTAELS
                     LYVDLEEQHLVPLLRKHAGTKALAPDAAKGNKELRAKLAELAVAPKDDDAFLEKLNEL
                     KKGFQQHVRNERKELLPAVLKALDDEEANQLAAAIEGGIDEAENAKREQKKQEAAAAK
                     REAEQAEAAAAAERETARAAKAAERQAREAADKMAESLERGAAAAKQTAQSVTVDLME
                     QTQQAATGAREAMTAYSGTVQKAAEDLRAVSASTNIAATGVSQFVSAWFEWAGKVART
                     NAEVSRRMLQSRNVTQFAEAHQEYVTSATRNLIEGNSALLEIAQRTSKEALRPLEGQL
                     AR"
     gene            20684..21235
                     /locus_tag="VF589_05790"
     CDS             20684..21235
                     /locus_tag="VF589_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257123.1"
                     /translation="MTTPKALVALVCSALLGAAASAAPANLFIKTGETIVVRVGADGA
                     MSVESRAAAPPLSGFEQSALAQMQAALAQVPPDAKTLPAMPILGPKDTKVPDIRPNSV
                     RLTFRAVAGGSEAMLWIENGYDGAFRYRAVMHRGERSAPTDVCQVMPKKPGAEHWPFQ
                     IERLELSEARLEPWQDGQGIRCQ"
     gene            21288..23060
                     /gene="recQ"
                     /locus_tag="VF589_05795"
     CDS             21288..23060
                     /gene="recQ"
                     /locus_tag="VF589_05795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX8257124.1"
                     /translation="MSNPLSILRSTFGFSDFRGVQPAVIERVMAGQHTLAVMPTGAGK
                     SLCYQVPALARKGTALVISPLIALMHDQIRSADGFGIKAAALTSADDNRQETVARLRR
                     GELDLLYVAPERASGDGFRQLIDSIKLSLVAIDEAHCVSEWGHDFRPDYRLLRPLLDQ
                     FGEVPRLALTATADRQTRADILVQLGIPEDGLILAGFDRPNIRYHIRPRDGVGAQLKT
                     LLAEQPGPGIVYVPSRDKAERLADQLSGPGRRALPYHAGLEPQVRARNQAAFVRSENM
                     VMCATIAFGMGIDKPDVRFVAHAGLPKSIEAYYQETGRGGRDGDPAEAWLFWGAEDFS
                     RARRRIETEVPEERRQGERQRLNALAAVVETAGCRRALLLRHFGETPPERCGNCDNCL
                     APPSTVDATEAARKLLSAAFRTEMRFGIAHLIDVLAGKETDKIHGFGHHRLSVFGIAE
                     GDELALMKPVARALMARDALRADEYGGLSFGPAAKPILKGEEKLDLVLPPRRQRKARR
                     GGAGGGNPEGDPLFEALRACRRDLAKEASVPPYVVFHDSTLREMAELRPTSLQALSRV
                     SGVGAAKLERYGKAFVEVVREHSS"
     gene            23057..23476
                     /locus_tag="VF589_05800"
     CDS             23057..23476
                     /locus_tag="VF589_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEX8257125.1"
                     /translation="MRGRLFPLDPDTLVSGQIMPEDLSALAAEGVTMIVNNRPDGEEP
                     GQPAAVAIEAAARAAGIDYRWVPVASGFSPDQVEAMGAALEAADGKVLAFCRSGTRST
                     FLWALARSRAGDDGDELVRKAAAAGYDLSPIRGLLQR"
     gene            complement(23467..23673)
                     /locus_tag="VF589_05805"
     CDS             complement(23467..23673)
                     /locus_tag="VF589_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX8257126.1"
                     /translation="MALVELALFQNPVAAEVARGRLASEGIETVLFGAGLASLGLGAM
                     TPARLMVDERDLKAAQTILAEDQR"
     gene            complement(23673..24206)
                     /locus_tag="VF589_05810"
     CDS             complement(23673..24206)
                     /locus_tag="VF589_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX8257127.1"
                     /translation="MAWQDDVLGFWFGLEPDQWWKADRELDRSCRARFLDLWEKQRGL
                     PADHYLGSARDAVAAVILFDQFPRNMFRGHAEQFATDPLALAVARGAVDRRIDRELTP
                     AERGFLYMPFQHSEDPGDQQRSLALFNALGDPHQLGFAVKHHDVIERFGRFPHRNPIL
                     GRTPRPDEVAAGDVFPW"
     gene            complement(24220..25650)
                     /gene="tldD"
                     /locus_tag="VF589_05815"
     CDS             complement(24220..25650)
                     /gene="tldD"
                     /locus_tag="VF589_05815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX8257128.1"
                     /translation="MTKLADPRRFLYRPDGLSPDAAQRLTAEALAACDDGELYLQYTA
                     SESFGFDDGRLKNADFNTQAGFGLRGVAGETTAFAHANELSEAAIRRAGETMTVLDPS
                     SASKAAPPRRTNATLYTDADPLSAVPFARKVALCQEIDAAARARDPRVAQVSVSLAGS
                     WSVIDIVRADGFVAHDVRPMVRLNVQIVVEANGRRETGYHGLGGRRLYDDIFEPATWN
                     RAVDIALAQALVNLDSIAAPAGEMTVVLGPGWPGVLLHEAIGHGLEGDFNRKGSSAFS
                     GRIGDRVAAPGVTVVDDGSIGARRGSLTIDDEGTPTRLNTLIEDGILKGYIHDRLNAR
                     LMGVEPTGNGRRESFAHAPMPRMTNTFMLGGNDDPGEILARAKNGIYAKSFGGGQVDI
                     TSGKFVFSCTEAYKIENGRLGAPIKGATLIGDGPTVLTKVAAIGNDMALDEGIGICGK
                     GGQSVPAGVGQPTLLIEGLTVGGTAA"
     gene            complement(25772..25978)
                     /locus_tag="VF589_05820"
     CDS             complement(25772..25978)
                     /locus_tag="VF589_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX8257129.1"
                     /translation="MTDDPFVPEISYVDTPRVACDGASQIHPALGHPRVFLQIDESGY
                     VECGYCDKRFVLRGGPADRGEQTA"
     gene            complement(26024..26929)
                     /locus_tag="VF589_05825"
     CDS             complement(26024..26929)
                     /locus_tag="VF589_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257130.1"
                     /translation="MRSIVAKVVQAALLLAGTASAEPPRHAEDDPEIVVTGTRDNKCA
                     ASDFIDSIMRDHDNQLARFHARICPATIGLPEGHAAAVMNRIRDVAATAGLELAGPKC
                     RPNLVVVVADGGRDFVTAFRKKRPRLFHDLRRRETAELFAAGAPARAWQIVERREADG
                     RVAEPFVEGVGYRPEAHLMPHMQSSRLQKSTRSDLALSFVVFDIAGVEGLTLTQLADY
                     AAMRALASTRPPAKPGGPRSILRLFDDPKQGIAPAAVLTKWDLAYLKALYATNGTMMA
                     WQERGRMVRRVRQELAGTGAEPERR"
     gene            26989..27168
                     /locus_tag="VF589_05830"
     CDS             26989..27168
                     /locus_tag="VF589_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257131.1"
                     /translation="MTAVEEWTALVEKRQAIGSEKAAIRASQAAQIAESNNARREKPR
                     RGRLPNMAAMMGAEP"
     gene            27249..28178
                     /locus_tag="VF589_05835"
     CDS             27249..28178
                     /locus_tag="VF589_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8257132.1"
                     /translation="MSSAAAISIDNLQKTYAGGKRALDGVSLDVPRGQIFGLLGPNGA
                     GKSTMINILAGLVNKTGGTASIWGFDIDEHPRNAKASIGIVPQEILFDPFFTPAETLE
                     MQAGLYGVPKAKRRTAELLRAVHLEDKARAYSRTLSGGMKRRLLVAKAMVHSPPILVL
                     DEPTAGVDIELRQQLWNYVRELNSRGVTIVLTTHYLEEAEQLCDRIAIINHGRLIANE
                     ATRTLVGMALEKAVEVTVDRDLTQAPNAPCFDKIELIGERTLAITYLKNRVNAGDVID
                     ALQREGLGIVDVSTREADLEDVFLSLTRASNAG"
     gene            28219..>29758
                     /gene="nadB"
                     /locus_tag="VF589_05840"
     CDS             28219..>29758
                     /gene="nadB"
                     /locus_tag="VF589_05840"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX8257133.1"
                     /translation="MNHSFDVVVIGSGAAGLSAALNLAPHLKVAVLAKGALSGGSTAW
                     AQGGIAAVLEPGDTFESHIEDTITAGAGLNHRRAVEFVVENAPAAIERLAELGVPFNQ
                     GGSERWHLTREGGHSHRRIVHVADATGWAVQQALEEAAALHPNITLVPDMVAIDLATG
                     RHAEASASENCVWGVYAVNRRTGGVDLFTGRATILATGGASRAWLYSTNPRGSTGDGI
                     AMAWRAGCRVANMEFNQFHPTCLHNVEVKNFLITEAMRGEGGRLTIPGSGHRFMPDFD
                     PRAELAPRDIVARAIDHELKRLGIDYVHLDISHRDPDFVRSHFPTIAERLAQPDIGID
                     ITREPIPVVPAAHYTCGGVVVDLDGKTDLDGLYAAGEVTMSGLHGANRLASNSVLECL
                     VFGLAAAHRINQGWSELPPPPPIRPWDESRVTHSDEEVVVHHNWREIRRFMWDYVGIV
                     RTTKRLERALNRVELLRQETEDYYGHFRVTPDLVELRNLVEVSGLIVRSALARKESRG
                     LHYTL"
BASE COUNT         4780 a         9791 c        10386 g         4801 t
ORIGIN      
        1 ccagaccaag cccttgcttg ccgtcctgaa gggcgggaag gcggacagac gtccggtctg
       61 gctcatgcgc caggcgggcc gctatctgcc cgaataccgg gagctgcggg ccgagaaggg
      121 cggcttcctg gcgctggtct atgacagcga cgccgcggcg gagatcacgc tccagccgat
      181 ccgccggttc ggcttcgacg gggcgatatt gttctccgac atcctcatcg tgccccatgc
      241 gctgggccag gacctcaagt tcgaggccgg cgaggggccg cggctgtcgc ccccgctcgt
      301 cgatgccgcg ctgagggcgc tggaagccgt gccagcgcgc ctaaacccca tttttcggac
      361 ggtggataag gtcaaggccg cgctgcccgc tgaggtcact ttcctgggct ttgcgggcag
      421 cccctggacg gtcgcaacct acatgatcgc ggggcaaggc agcaaggaac aggccgaggc
      481 gcggcgctac gcctatcgcg atccgcaggc cttcgccgcg atcatcgacg cgatcgccga
      541 gatgaccgtc gattacctgg ccggccagat cgaggccggg gtcgaggcgg tgcagctgtt
      601 cgacagctgg tcgggcagtc tcgcccccgc ccagttcgag caatgggtga tcgcgccgac
      661 cgcgcggatc gtggcagggc tcaagcagcg ccacccgcac gtgccgatca tcggctttcc
      721 caaaggcgcc ggcggcaagc tcgccgctta tgcccgcgag accggggtgg atgcgatcgg
      781 gctcgacgaa acggtggatc ccgaatgggc cgcggccgct ctccccaagc agcttccggt
      841 gcagggcaac ctcgacccgc tcgcgctgat cgccggcggc gaggcgcttg aaaaggcggt
      901 ccagcgcatc atatcagcct tcgaagaccg cccgcacgtc ttcaacctgg ggcatggcat
      961 tttgccggat acgccgatcg cgcatgtcga acagctgctt cgcctggtgc gccagtgatc
     1021 gccaatgacc tcgaaacggc tctttctttg acctacgcct ggatcaaggc cgcgcacgtc
     1081 atcttcgtga tcttctggat cgcggggctg ttcatgctgc cgcgtttcta cgtctaccac
     1141 caggaagcga cgccgggatc ggtcgaggag cagcgctggc tcgaccgcga gcgcaagctg
     1201 cgcaacatca tcatcacccc ggccatgctg ctggtgtggg tgttcggcct gacgcttgcc
     1261 tatatcaccg gcgcctgggc ggagggttgg ttccacgcca agttcgcttt ggtgctgctc
     1321 ctgtccggct atcacggcta tatggtcggc tatggcaaga agctcgcccg cggccagcgg
     1381 ccggcgagcg gccggtcgct caggatcatg aatgagatcc cgggcatcgc caccgcgctg
     1441 atcgtcatcc tggtgatcgt tcgtcccttt tagtccgctt gacttggttt cggccggagc
     1501 ttaacccaaa ctaagtcgtt agcctgccgt tcgcgcagtc tcgcccccca cccgacagcc
     1561 gatcctcgcg ctccggacga cattattcag cttttccgag aatcctatgc atctcaaaca
     1621 attgaaacag aaaaccccgg ccgatctggt cgcgatggcc gaggaactcg gcatcgaggg
     1681 cgcgtcgacg ctgcgcaagc aggagctgat gttctccatc ctcaaggtcc aggccgagaa
     1741 cggccaggag atcatgggca tggggaccat cgaagtgctg cccgacgggt tcggcttcct
     1801 gcgcagcccc gaggccaatt atctggccgg ccccgacgac atctacatat cgcccaacca
     1861 ggtgcggaag ttcggcctcc ggacgggcga cacggtcgag ggcgagatcc gcggtcccaa
     1921 ggacggcgag cgctatttcg cgctgactcg gctgatctcg gtcaattacg acgatcccga
     1981 cgccgtccgg caccgcgtca atttcgacaa tctgacgccg ctctatcctg acgagaagct
     2041 gacgctcgac accaaggatc cgaccgtcaa ggacaagtcg gcgcgggtca tcgacatcgt
     2101 ctcgccgcag ggaaagggcc agcgcgcgct gatcgtcgca ccgccacggg tcggcaagac
     2161 gatgctgctg cagaacatcg ccaaggcgat caccgacaac catcccgaag tgtttctgat
     2221 cgtcctcctg atcgacgagc ggcccgagga agtcaccgac atgcagcgct cggtgaaggg
     2281 cgaggtgatt tcctcgacct tcgacgagcc cgcgcagcgc cacgtccagg tcgccgagat
     2341 ggtgatcgaa aaggccaagc gcctggtcga gcataagaag gatgtcgtca tcctgctcga
     2401 ttcgatcacc cgcctgggcc gcgcctacaa cacggtcgtg ccgagctcgg gcaaggtgct
     2461 gaccggcggc gtcgatgcga acgccctgca gcggcccaag cggttcttcg gcgccgcgcg
     2521 caatatcgag gaaggcggct cgctctcgat catcgcgacg gccttgatcg ataccggatc
     2581 caagatggac gaggtcattt tcgaggagtt caagggcacc ggcaattccg agatcgtgct
     2641 cgaccgcaag gtttccgaca agcgcatctt cccggcgctc gacgtcggca agtccggcac
     2701 gcgcaaggag gagttgctgg tcgacaccgc caagctttcc aagatgtggg tgctgcgccg
     2761 gatcctcatg cagatgggga cggtcgacgc gatgcaattc ctgctcgaca agatgaagga
     2821 cgccaagtcc aacgaggatt tcttcgcgtc gatgaaccag taagccgaag aggcgttcgg
     2881 gggcgagatg atggagctgt tcttcaacct gcaagcggcg gtgccgatgg gcatcggcgg
     2941 catctgggac gccatcgtcc tcgatttctc gaaactcaac accgccgcgg cctgggtggt
     3001 cttcggccag gtgctgatga tcgacatcat gctcgcgggc gacaatgcga tcgtggtcgg
     3061 cgcgctcgcc gccggactgc cgccggagca gcgccgcaag gtgatcctga tcggcatcat
     3121 cgccgctttg gtgctgcgca tcgccttcgc cctggtcgtc acccagttga tgcagatcgt
     3181 cggcctgacc ttcgcgggcg gcctgctgct gctctgggtg gcctggaaga tgtggcgcga
     3241 gctccagcat tccggccagt cgcaagggtc ccctgaaatc aggggcgatg aggatagcgg
     3301 cctcaagccc gccaagagct tcgccggcgc cgcctgggcg gtggccgtgg ccgacgtctc
     3361 gatgagcctc gacaatgtgc tggcggtggc cggcgcggcg cgcgagcatc cgggcatcct
     3421 gatcatcggc ctgttgctcg ccgtcgcatt gatgggcctc gccgccaaca tcctggccaa
     3481 atatatcgag cgctatcgct ggatcgcctg ggtcgggctg ctggtgatcg tctacgtcgc
     3541 cggcaagatg gtctatgagg gctttatcga ccctcaggtg gggctaggca cactgttcgc
     3601 ctgaccttcg gccggcgctt cgtcgaagca ccggcgccgt tggacgaact ttcaagaccg
     3661 ctggcgccgc cgccgcgatg agggtagcat agctctcatt ggaggttcgc gcatgggccg
     3721 tcacctgctt ccgttcggct tcgctgccgc ggcactgacc gcctcattgt ccgcggcgga
     3781 gcctcctgcc gcgccgactc gggccggcgc cgagcgcgac gtcatcgcct tcgaacagga
     3841 caccgccgcc cgcatgacgg tgccggtcag catcggcggc cgcggcccct atcgcttcat
     3901 cgtcgatacc ggctcggaac ggacggtcat ttcggaggag ctcgctcgca acctcgagct
     3961 cagcgccggc cgcgaggcta cgctgcacag catcagcggc gcgggcgagg tctcgaccgt
     4021 ggtgatcccc aatttgcgcg tcagcaagcg caacgtgcgc gggatccacg cccccgccct
     4081 agccgccgcc gatctcggcg cggtcggcat gctcggggtc gatagcttga aatcgcagcg
     4141 cgtgctgttc gacttcggcc gccagaccat ggccgtgacc ccgtccgtcc aatatgagca
     4201 ggattggggc cccaacacga tcgtgatcaa ggcgcgcacc cgctacggcc gcctcgtcct
     4261 ctccaacgcc cggatcgaag gcgagaaggt gctcgtcatc ctcgacaccg gatccgaagt
     4321 gacgatcggc aacgaggcgt tgcggcgcaa gctcgctgcc cgcggcaagc ttcgttcgac
     4381 gcggccgatc agcctgctca gcgtcaccgg cggcctcgcc cccgccgatt acacgaccat
     4441 cagccacgtc cggctgggcg gcgcctatat caagcagctg ccggtcgcgt tcgccgacat
     4501 ccacccgttc cggcagctcg acctgctcga tcggccggcc ttgctgctcg gcatggacgc
     4561 gctgcagctg ttcgaccggg tgtcggtcga cttcgcccac cggcgcgtgc aattcctcgc
     4621 gccggatctc ggcctgctcg aggagcagac ccggatggcg gcgctggcgg gcggatcccg
     4681 caccgcctcg cgctgagcta aggcaccagg atggtcgagc cggtcgtcgc ccgcgcctcg
     4741 agcgcgcgat gggccgcggc ggcgtcgctg agcttgtacg tggcgccgat gcccggccgc
     4801 acgctgccat tgccgatcat ctcgaacagg cgggcggccg aggcctccat ttcgccgggg
     4861 gcggcgatat aatcggcgag cgtcggccgg gtgacgaaga cggagcccgc cttggtgagg
     4921 tcaagcgcgc ggaacggcgg caccgggccc gaggcattgc cgaaggtgac gagcaggccg
     4981 cggcgggcaa gcgagcccag cgaggctgcc cagctcgccg cgccgacgcc gtcgaggacg
     5041 atatcggcgc cctttccgcc ggtccgctcg cgcaccgcgt ccgccagcgc gcccatcgga
     5101 caatcgagcg catgatcggc gcccgcttca gccgccagcg ccgctttggc ttcgctgccg
     5161 gcatgggcaa tgaccgtcgc gcccaaggct ttcagccact gcaccaggat cgagccgacc
     5221 cctccggcgg cggcatggac caggacggtt tgcgccgctt gcacccgggc gcagcgctcg
     5281 atcaggaatt cggccgtgca tcccttgagc atggccgcag ccgcgatttc gggagcaatt
     5341 ccggtaggca gccgcaccag gcggtccgcg gcaatcgtcc ggtgcgtcgc ataggctccc
     5401 agcggcccgg agaaatagcc gacccggtcg ccttcacgga agccgctcac ctccgggccg
     5461 acagcctcga ctaagccggc tgcttccgac cctaaaccgg aggggagggt cagcgggtag
     5521 agcccagaac ggtaataggt atcgatgaag ttcaacccca ccgcctcgtg acggacgagg
     5581 atctccccct cggctggcgc ggtgagctcc atcggctccg gctcgagtat ttcggggccg
     5641 cctgttcggc ggagggtgaa gctatagggc ggtcccatca tctgcctccc ctcgttcttc
     5701 gttccgccgc taaccgagat gctcggcgaa aaacgcttcc gttcgctcgt ccgccagccg
     5761 cgccgaatcc tcgctgcgcc tcttgcccat ctcggcggcg aagccgtgat cctcgccggg
     5821 atagtcgtgc aagacgactt tggggtggcc gtccagagcc tcgtggatcc tggcctgcac
     5881 gtccttgggc acgaagccgt cctcgccggc gatttggagc atcagcggac gggcgatcgc
     5941 ctccttgtcg ccgagcaggc tgtcgaggcc gacgccgtaa tagccgacgg tcgcgtcgac
     6001 atcggttcga atcgacgtca tgaaggcgag ccggccgccg aggcaatagc cgacgcatcc
     6061 tacttttccg tttttattca ggcgcttacg ggcggcgcca agggtcgcct cgatgtccct
     6121 gacgccttgg tcctggttga acttgcccat caggtccagc gcctgcttga attcctgctc
     6181 cacgtcggga tcgagctcga cgccgggctc cagccgccag aacaggtcgg gcgccagcgc
     6241 cagatagccg agccgcgccc aatggtcgca cttgcgccgg atgccggcgt tgacgccgaa
     6301 aatctcctgg atgaccacga tcgccgcgcg cggggggccc tccggctcgg ccagataggc
     6361 cttgaagcta ccgtcctgct cgagcgtttc gatgtcgatc atgcccatgg atgcgggtcc
     6421 ctgtcttgcg gcggcggagc ggactataga catagagcag gccacagccg caagcagccc
     6481 gagaggtgag tgccatgaaa gttacggtcg atgtcgattg cactccggag gaagcccggc
     6541 gcttcatcgg actccccgat ctcagcccgg tccaccaggc atatgtcgag aagatgcaga
     6601 aggcggtgac cgacgggctc actcccgatg cggtcggcga gatgatgaag gcgtgggggc
     6661 cgatgagcga cgccgggatg gcgatgtggc ggcagatgct ggagcggatg ggcgggacca
     6721 aggcctagcg tccgcaagcg gggcgggagc gaatggcggc cgacactatc tacgccctct
     6781 cctcgggaag cccgcccgcg gggatcgcgg tggttcggat cagcggaccc ggtgcggacg
     6841 cggcgctgat ggcgcttgcg ggacggcttc cggagccgcg gcgggcgggc gttgccgcgc
     6901 ttcgtcaccc gctgagtggt gagctcctcg accgggcgct ggtgcttcgc ttgccggggc
     6961 cggggagcgc gaccggcgag gacgtggcgg agctccatct ccacggcggg cgatcggtgg
     7021 tcgccgctgt cctcggcgcg ctcgcggcgg tgccggggct gcggcaggcg gagccggggg
     7081 agttcacccg gcgggcgttt gaaatcgggc gcatcgatct tgccgaagcg gagggcttgg
     7141 ccgacctgct ggcggcggag acagaggcgc agcgccgggc agcgctccag ctcgctggcg
     7201 gggcgctgag ccgggaggtg gagcgctggc aggcggagct gctcggcctc gccgccgagc
     7261 tcgaagcggc gctcgatttc gccgacgagg gcgatgtcgg ggcggaccta ccggcggggt
     7321 ggcgggagcg gctggcgcga ttgtgcgggg agttgaaggc ggcgcttgcg cggccgccgg
     7381 cggaacggct gcgcgacggc gtccgggtgg tcctggccgg gccgcccaat gccggcaagt
     7441 cgagcctgtt caacgcgctg gccggccgcg aggcggcgat catctcgccg ttgccgggca
     7501 ccacccgcga cctcatcgag gcgccggtgg cgatcgcggg gatgccgttc ctgctcgtcg
     7561 acacggcggg gctgcgcgag agcggggacg ccattgaggc gatcggggtg gaccgggcgc
     7621 ggtcgagcct cgccgccgcc gacatcatcc tgtggctggg gaatccaggg gcggccccgg
     7681 accgggaccg gacccttgtc gtcggggcaa aggccgatct ggggccgccg ccgccgggga
     7741 ccgatctgcc ggtctccgcg atcactggcg aggggatcga gcggctggtc gactgcctgc
     7801 gcgagcgggc aaagtcgctg ctccccgggg agggcgagat ggcgatcaac gcccgtcatc
     7861 gcggcatcct ccgggatgtg ctcgcgagtc tcggggaggc aggggacacc gacgatctgc
     7921 tcatagcggc cgaatcggtg cggctggggc ggctggccct ggaccgcctc accggccgcg
     7981 ccggggtgga ggacatgctc gacgctttgt tcgggcgctt ctgcattggt aaatgaaatg
     8041 ttccacgtgg aacagcgtcg aagcccgctt cccggactcg ccggcgcggg ttagacgggc
     8101 gccatgacgc gcttcgacat catcgtgatc ggcggcggcc atgccggctg cgaagctgcg
     8161 gccgcggccg cgcgcatggg agcgtcggtc gcgctcgtca atttcaaccg cgacacgatc
     8221 ggcgcgatgt cgtgcaaccc ggcgatcggc ggtctcggca aagggcatct ggtgcgcgag
     8281 gtcgacgcct gcgacgggct aatcgcccgc gccgccgacc acgccgccat ccactatcga
     8341 atgcttaacg cgagcaaggg tgcggcggta cgcggccccc gcgtccaggc ggaccggcgg
     8401 cgataccgct cggccatcca cgacctgctc gccgggcaat cgggtctcac catcgtcgag
     8461 ggtgaggcga gcgcgttgcg gctcgagggc cgccgcgtca ccggactgac cttggccgac
     8521 ggccgagaac tagcggcgac ggcggtggtg ctcgccaccg gcacgttctt gggcgcgaag
     8581 cttcatttcg ggatgacgag ctgcgccggc ggccgggtcg gcgagcgcgc ggccaccgag
     8641 cttgcggggc agcttcgcgg cctgggcctg ccgctggccc ggctgaagac gggtaccccg
     8701 ccgcgcctcg acggccgcac gatcgactgg agccggttgg agcggcagcc gtccgacgac
     8761 ggtggctgga ctctctcggc catgaccgaa gcgcgggcgc cgccgcagct gttctgcgcg
     8821 atcacccgca ccaatagccg cactcacgag atcatccgcg cctcgctcga tcgctcgcct
     8881 ttgttcgggg gcgagattac cggggtgggg ccgcgatatt gtccttcgat cgaagacaag
     8941 gtccatcgct tcgccgaccg tgacggccat caagtcttcc tggagcccga ggggctggac
     9001 gatccgacta tctatccgaa cggcgtctcg acctcgcttc ccgccgacgt ccagctcgcc
     9061 atggtgcggt cgatggaggg gctcgagcgg gccgagatcc tgcagcccgg ctatgcggtc
     9121 gaatatgatc atatcgaccc gcgcatcctc gacccgacac tggcggtgcg cccggagctc
     9181 gcgcaggggc tttacctggc gggtcaggtc aatgggacta ccggctatga ggaggccgcc
     9241 gcccaggggc tcgtggccgg ggtgaacgcc gccgcctggg tcgccggcaa gccgccaatg
     9301 ctgttcgatc ggagcgagtc ctatatcggc gtcatggtcg acgacctcac cctgcagggt
     9361 gtcaccgagc cctaccggat gctgacggca cgcgccgaat atcgccttcg gcttcgcgcc
     9421 gacaatgccg acgtccggat caccccccaa gcgattgccg cggattgcgt atcgcaccag
     9481 cggcggaaga tgttcgtggg gatgcagcag ggccgtgccg atatcgaaga gctgcttggc
     9541 caacgcttca cggcgaaaca gctgctggcg gcgggtgccg aggttcggga cgagggtgtc
     9601 tcccgcaccc tggccgagtg gactcgcttc ccgaacgtcg gggcagatgc gctcgtgcgg
     9661 atcgcgcccg ctcttgccgc agccgatcgc aagctgctcg acagcgcagt cgaggatcat
     9721 cgctacgcgc catatctgga ccgccagcag gccgaggtgg cgaggctgaa ggcggacgag
     9781 gcggtgcgga tcccggccca cttcgattat gggggcgtac ctgggctttc caacgagatg
     9841 gtcgagcgct tatcatcggc ccgtccgcac agcttgggcg cagcccggcg cgttcgcggg
     9901 atcacgccgg ccgcgttggc ctctatactg gtccatctgc gtcgtaaggc ggcgtgatgg
     9961 atgaagacga ggcacgccag cggctcatcg gcgagttcgg tgttccacgt gaaacactcg
    10021 cccggttgaa gctgtttgtt aactatttgg cggctgagaa cgagcgacaa aacctcgtct
    10081 cccgcgcgtc tctcgacgag gtttggtcgc gccatatcct ggattcggcg caacttctca
    10141 gcttcgctcc taggtcagtg ggatcatggc tcgacttggg cacgggggcc gggttccccg
    10201 gcttgatcgt tgcagccttg cgacgcgata tagcggttac catggtcgag gcgcgccggt
    10261 tgcgcgtcgc cttccttgag gctgcggccg acattctccg gttgaggccc gacaccatca
    10321 ttcgctgcgc caaggtcgag caggttgaat ccgccccttt cgacgtgatc agcgcccgcg
    10381 ccttcgcgcc tttggaccgg ctgttggcgc tgggcgagcg atttgccacc gcaaatacac
    10441 gttggatcct gcccaagggc aaaaatgcca aaacggaact ggatgctgcc gaatcgctgt
    10501 ggcagggtag ctatcgtatc gaaccgagcc tcaccgacgc cgatgccggc atcatcgtcg
    10561 cggaaaatgt tcggcgaaag gccagggcac gaagatgatc cggattgcgg tcgccaatca
    10621 gaagggggga gtgggcaaga ccaccacggc gatcaacctc gccaccgcgc tggcggcgac
    10681 gggctggaag gtgctgctgg tcgatctcga tccccagggc aatgcctcga ccgggctcgg
    10741 cgtggcgcat cacgagcgca atcagtcgag ctacgaattg ctgaccggcg gttgcacgct
    10801 cgaggaggcg gtggtcgcca ctcggattcc gcggctggac ctggtccctt cgacggtgga
    10861 cctctccggc gccgagatcg agctggtcga atatgaggag cggacgcacc ggctcgacaa
    10921 gagcttgaac agcgcgtccg gcggccgctg ggacatttgc ctcgtcgatt gcccgccttc
    10981 actcggtctg ctgaccatca acgccctggt ggcggcgcgc gccatcctcg tgccgctcca
    11041 ggcggagttc ttcgccctcg agggattgag ccaactgctc cagacggtcg agcgggtgcg
    11101 ggctcggttc aaccccgagc tttccatcct cggcgtcgca ctcaccatgt acgaccgccg
    11161 caacaatctt tccgcgcagg tcgccgctga cgtccgcgcc tgcctcggaa aggtggtgtt
    11221 cgataccgtc atcccgcgca acgtgcggct ttccgaagcg cctagccacg gcgtgcccgc
    11281 cctcatctac gatcatcgct gctcaggatc ggaggcttat atgtcgcttg cgcgcgaact
    11341 catcggccgg ttgccggcgc cggcggccgc ggcatgatcg acgagacccc gatccgccgc
    11401 cgtgcgcccg gtctcggccg cggcctctcc tcgctcctcg gcgaagtcgc gcaggaagcg
    11461 ccgattgccg gaggatcggg tcggggcggc atccagatgc tgccggtggc gagcatcgaa
    11521 cccaatccga accagccgcg ccgaagcttc gacgaagatg cgctgacgga gctcgccgag
    11581 tcgatccgca cgcgggggct gatccagccg atcgtcgtgc ggccgcgcga gggcggctat
    11641 cagatcgtcg ccggcgagcg ccggtggcgg gcggcgcagc gcgcgcgcct gcacgacgtg
    11701 ccggtgatcg tccgcgactt tgacgatgtc gagacgctgg aagtcgccat catcgagaac
    11761 atccagcgcc aggacctgaa cgcgatcgaa gaggccgagg cttataagcg gctgctcgac
    11821 gagttcggcc atacccagga ggcgttaggc cggctggtcc acaaatcccg cagccatgtc
    11881 gctaacctat tgagattgct tgaccttcca tcgaaggtgc gggcttgggt cgcgtcgggg
    11941 gagctcagca tgggccacgc ccgcgcgttg ctggggacca gcgacccgga agcctgcgca
    12001 ctcgaggtga tcaaccgcga cctgtcggtg cgggagaccg agaagcttgc ccgtgccgac
    12061 aagccgcggc ggccaggccc gatcgaacat aaaggtgcga gcgccgacat cgaggcgctg
    12121 gagcggcagc tcggcgacat gctggggctg aaggtcagga tcacgcatcg gccggacggc
    12181 ggtcaggtca cgctcaatta ttcgtcgctc gaccagctcg acatgatctg ccagcgcctc
    12241 agcggcgagc gtatctgaac acgggacgca ccggctagcg cagtcgcccg gcttggcggc
    12301 agatcgcgaa cagctcctcg tccacggcca ccgggccaac gccgccggat gccttcacct
    12361 ggcgctccgc ttcaagcaga cggcccatgc tcttggccag aaggtcggat cgccagcgcg
    12421 agagctgcga ggcgaccgca tccttctcct tccagaagac cgccttgcca tgtgacgcca
    12481 tgacggcggc gacgctgttg ccgcgatcgg cttccgaccg cagcttcgcc agcaatgtca
    12541 tccgccggac cacggcacgg atcagcgata tgccctcgat tccctcgctt ttgagccgcg
    12601 ccatctcggt ctgcagcagg ccgagatcgc caccggcgac ggcttcgacc agccgcgaga
    12661 ggtcgccttc ctcattggcc gcgccgaccg cctcgaccgc ttcttcgtcg agcggcttcg
    12721 gccggtcggg agcggcgtcg agatagaggg cgtatttcgc cagctcctgt ccaatcagcg
    12781 cacgattgcc gcccgcggcg tcagcgatgc ggcgggcgac gtcgctgcgc atatcgaggc
    12841 cctgctcgcg cccaagctcg gtcacgagcc gcgccgcctc gcgcgcgtcg ggcaggtagc
    12901 tggcgaaggc catggccgag ggggcggcca aggcggcctt gaggagcttg gaggtgggct
    12961 tcagattgcc ggcgatcagg accaccgggt tgccggcggc cggcgcctcg agcagcgcct
    13021 ccaccgccgg cagcgcctcg tcgccggcgg gctccaccca gatgtagcgc gggtcgccga
    13081 acagtgagat ggaggcggcc tcgtcggcga gccgcgccgg atccgatttg aggtcggcgc
    13141 cgctgaggtc gatccgctcg gctcccgcgc ccattgcctt ggcgaggctg agcgcgagcg
    13201 cgcgcgatcc agcttcgtcg gggccgtaaa gcaacgtcca gcggatgtcg ggggacgggg
    13261 cccggagcat ctgttcgatc tggccgcgac tggccttcac cggccgccgc ccgtgcgcga
    13321 cacgtagagt gcgacccggg cgacgatctg gtccgcaacc tgggtcgaca gccgctcgag
    13381 cgcggtctgc tcggccgcga cggtggcata ttcggagccg accacgtcga tcccggcgtc
    13441 ggatccggca gtggcgtcga gcaggacagt gccgagcgag gcatcgacca ggcgatagcg
    13501 tgcgcgcaag gtccggcgct cccgggtcac cgcattgttg cggatgccga acccggtaat
    13561 gtcgtcgtcg agctcgactt cgagccggta gcgcggcgcc gcgccctcgg gtgcccgcag
    13621 ccggtcctcg agcgcggtgc ggacgagcca gccggagcga ccggcgatcg gcgccaccgt
    13681 gatcgagcga agcgtctgcg ccaccgggcc gcgttcgccg ccgctataga gcgggcgcag
    13741 gccgcagccg gacaggagca ggcccgtaag gaggagcgcc gcgcggatca cgcgaccaga
    13801 ttgaccaaac ggtccggcac cacgatcacc ttgcgcggcg gcttgccttc caggatgcga
    13861 accaccttct cggaggcgag cgccatttcc tcgagcgtct cgcgcgcggc gcccttggga
    13921 gcggtcagcg tgtcgcgcag cttgccgttg acctggacgg cgatggtgac ttcgtcctcg
    13981 gcgagcagcg tcggatcgac cgtgggccaa gcctggtcgg cgatcaggcc ttccctgccg
    14041 gcggcgctcc aagcttcttc ggcaaggtgg ggcaccatcg gcgcgacgag cagcagaatg
    14101 gtctcggcgg ccctggcgcg cgaggcggag ggcggcgcct tttcaatcgc gttggcgagc
    14161 tcgtagagat tggcgacggc cttgttgaag gcgagcgcct cgatattggc ggcaacggcg
    14221 gcgatggtct ggtggagctt gcggtcgagc gcccggtcct cccccatgcc ggtttcggca
    14281 ttgctgccga tgcgccacag ccgctggacg aagcgccacg acccttcgat gccggcctcg
    14341 ctccacggca ggtcgcgctc gggcgggctg tcggagagca tgaaccagcg cacggcgtcg
    14401 gcgccatatt ggtcgacgat cgtgtcggga tcgacggtgt tcttgcgcga cttggacatc
    14461 ttctcgacgc ggcccgccgt cactggctgg ccgctctcga tgtggaccca atcgtcgcca
    14521 tcgcggcgga cctcgtgcgg gctcagccag gagccgtcgc ccgccttgta ggtttcgtgg
    14581 gtcaccatgc cctgggtgaa caggcccttg aacggctcgt cgacggcgat gcggccgatc
    14641 cgcttcagcg cgcgcgtcca gaagcgggcg tagagcagat ggaggatcgc atgctcgacg
    14701 ccgccaatat attggtcgac gggcagccag ctctcggcga cgctcgcgtc gaacggccgg
    14761 tcgcccggct ggctggcgaa gcggatgaaa taccagctcg aatcgacgaa cgtatcgagc
    14821 gtgtcggtct cgcgccgggc ggcggccccg catttggggc aatcgacatg gctccagctc
    14881 tggtggcggt cgagcggatt gccgggaagc tcgaagctga cgtcctccgg aagcactacc
    14941 ggcagctggt ccttgggcac cggcacggcg ccgcaggctt cgcagtggat gatgggaatc
    15001 ggcgtgcccc agtaacgctg gcgcgacacg ccccagtcgc gcagccgcca caccacggtg
    15061 ccttcgcccc agccgtcggc ctcggcgcgg cggatcactt ccgccttggc gggctcgacc
    15121 tcaagatcgt cgaggaagcg cgagttgacg atgacgccgg agccggtgaa ggcctcgcct
    15181 tcgaccgggt catcggcttg ctgcgcggca ggggcgacca cgcgccggat cgggaggccg
    15241 tatttggagg cgaactcgaa gtcgcgctgg tcgtggccgg ggacgccgaa aatggcgccg
    15301 gtgccgtaat ccatcagcac gaaattggcg atgtagagcg gcagcttcca ctcggggtcg
    15361 agcggatgga tgacggcgag gccggtgtcg tggcccttct tctcggcggt ctcgagctcg
    15421 gcggcggtgg tgccgccgcg cttgcattcg gcaatgaaag tggcggccgc ttcattccgg
    15481 ccggccagtt cgagtgcgat cgggtgatcg ggggagatcg ccgcgaagct ggcgccgaag
    15541 atggtgtcgg ggcgggtggt gaagacttcg agcgagtcca tgccggcggc ggggcgctcg
    15601 agcgcgaagc ggaagcgcat gccgacgctc ttgccgatcc aattctcctg catcagccgg
    15661 accttgtcgg gccattggtc gagcgcgccg aggccttcga gcaggtcctc ggcgaagtcg
    15721 gtgatcttga ggaaccattg gttgagcttg cggcgctcga ccagcgcgcc cgaacgccag
    15781 ccgcagccgt cgatcacctg ctcgttggcg agcacggtca tgtcgaccgg atcccaattg
    15841 acctcgctct gcttgcgata gacgaggccg gcctcgaaca gatcgaggaa cagcgcctgc
    15901 tcgtggccgt aataatcggg ctcgcaagtg gcgaactcgc ggctccagtc gagcgcgaag
    15961 ccgagccgct tcagctgccc ccgcatggtg gcgatattgg cgcgggtcca ttcgccggga
    16021 tggaccttct tctccatcgc ggcattttcg gccggcatgc cgaacgcgtc ccagcccatc
    16081 ggatggagca cttcgaagcc cttcatccgc cggtagcggg cgagcacgtc gcccatcgta
    16141 tagttgcgga cgtggccgat atggatgcgc cccgaaggat aggggaacat ctcgagcacg
    16201 tagctcttcg gcttgccgct gtcgtccgag gcgcggaagg agccgcgctc ctcccagacc
    16261 tgctgccagc gggcgtcggc cgtccggggg ttgaagcgcg aagccattgc cgaacccgtc
    16321 ttagccgacg gcggcccgcc ggaggtcgcg cgccttggtg aggatgatct cttcgagctt
    16381 ctggacggtc gcggccgcga ccggcgcttc cacccactgg ccgttctgca tcacctggcg
    16441 cgaggcggcg acgcgcagcg cgtcggcgcg aaggtcgcgg tcgaggatgg tggcggtgac
    16501 cttcacccgc tcgcccgggg aagcggggtt ggcataccaa tcggtgacga tgacgccgcc
    16561 gttggaatcg acctgggcca aaggcgcgta ggacagggtg tcgagcgccg cccgccagag
    16621 ataggaattg acgccgatcg tcgtcacctg cgaggcggcg aggtcggcct ggggcagcgc
    16681 ccggttgcgc gagcaggcgc cgagcgtgac ggcgcaggcg atcgcgaggc cgagagaaac
    16741 agcgcggttc atctggatgt ccttgatgct tttcagtcgg ccgccgttat agataacgcg
    16801 gcgcgcgcgg caagccgtct ctgagcgcga accgggcctc cggccggcgc cgggcgcggg
    16861 aatcagagct tggcgagctg ctgcggtttt acggtagcgc ggtcgatgcg ctgctgctgg
    16921 ccgggccgcg gctcgggcag gtcgacctgc tcgggcatca aagtctgcga gatggcaccg
    16981 gcggcgggct tgcactgggc gacgacgacc gggaaggcga cgtcgctgtt gttcatcagc
    17041 gcgtcttcgg tggcggcgtt gcaggcttcg acgctctcgt agcgggccgg cgcgacggca
    17101 acctgctggc aggccgtatc agcctcgccg cagcccatga tcgccatgat gtaaaaggct
    17161 ggacccatgt ccgtgctccg ctctgcccga aaccgttaaa ctgctggaga atcacaatgt
    17221 tccaattgag tctcacgcgt aacttggtgg cgggtccccg catcactatt tagaccaaca
    17281 tgccgcccga aacgacgaac ccgaccgaac ggccggaagc cgggtggcaa gcgctgcgcc
    17341 gcttcctccc ctatctgtgg cccgcgggcg agccggcgct ccgggcgcgc gtggtggcgg
    17401 cgctggcgct ggtgctgatc gccaagggcg ccaccctgat catgccgttc gcctacaagg
    17461 cggcgatcga ccagatggcg ccggggatgg aggcggcggc gggcctcgcc atggcgttcg
    17521 tcgcggccta tgccggcgcg cggttcggcg gcgtgctgtt cgacaatctc aggaacgcca
    17581 tcttcgaacg ggtcgggcag gatgcggcgc ggcggctggc cgagcatgtc ttccgccacg
    17641 ttcacaaatt gtcgctgcgc ttccacctcg agcggcgtac cggcgcgctc accaagatcg
    17701 tcgagcgcgg caccaagagc atcgacatga tgctctattt cctgctgttc aacatcgccc
    17761 cgactatcat cgagctgatc gccatctgcg tcatcttcgg cgtgaagttc ggccccggtt
    17821 tggtggtcgc gacgctggcg atggtggcgc tctatatctt cttcacgcgg ctggtcaccg
    17881 actggcgttc gcaactgcgg cgcgaattga acgacatcga caataaggcg atcgcccgcg
    17941 ccgtcgattc gctgctgaac tacgaaaccg tcaaatattt caccgccgag gagcgcgagg
    18001 cgcgccgcta cggcaaggcg atgcgcgcct atcaggaaag cgcgatccgc aacgagacct
    18061 cgctcgcatg gctgaatatc ggccagtcgc tgatcaccaa tgtcatgatg gccggcgcca
    18121 tggcctatac ggtgtggggc tggagccgcg gccagttcac caccggcgac gtggtgctgg
    18181 tcaatacgct gctggcgcag ctcttccgtc cgctcgacat gctcggctgg gtctatcgca
    18241 acatccgcca gggcctgatc gacatggagc agatgttcga catcatcgac actcaggccg
    18301 aggtgaagga caagcaaggc gcgcccgagc tccgggtcgg cgccggccat gtccggctcg
    18361 agggggtgcg cttcggctat gagcccgatc gcgagatcct caagggcatc gacctcgatg
    18421 tgccgccggg aacgatgctg gcggtggtcg ggccgtcggg cgcgggcaag tctaccttgt
    18481 cgcgcctgct gttccgcttc tacgagccga ccggcgggcg catcaccatc gatgggcagg
    18541 atatatgcga ggtcaaccag gcgagtctgc gcgccgcgat cggcatcgtc ccgcaggaca
    18601 gcgtgctgtt caacgattcc atcggctaca atatcggcta tggccgcgat ggcgccggcc
    18661 aggcggagat cgaggccgcc gccaggggcg cagcgatcca cgacttcatc atgggccttc
    18721 cggccgggta tgacagccag gtcggcgagc gcgggctcaa gctttcgggc ggcgagaagc
    18781 agcgggtcgc gatcgcccgc accttgctca aggacccgcc gatcctgatc ctcgacgagg
    18841 cgaccagcgc gctcgacagc cgcaccgagg cggcgatcca ggagactttg cgcagcgtcg
    18901 ccgcccgccg caccgccatc gtcatcgccc accgcctctc caccgtcgtc gacgccgacc
    18961 agatcgtcgt cctcgacgag ggccgcgtgg ccgagcgcgg cacccacgcc cagctgttgc
    19021 ggaagggcgg cctttacgcg gagatgtggg cgcgccagca gagcgagcgc gagcaggacg
    19081 cggccgaggc ggcggaatag ggcgccgggc ggcgttcagt cccaatccgt cattccagcg
    19141 aaagctggaa tcccactggc cttttggccg ccacaagaag ggagaaccca gctttcgctg
    19201 ggatgacgtt ccgtgccttg ctccggccca ggggataacg gagacccgag ttttcgctgg
    19261 gatgacgttt ctcggtcacg acagggaact aacctcggtt aggaacgcta tgttgcattg
    19321 cagcatgggc tatcccgcgc tgcacaacca ttccacggcg ttcgccacgg gcgtcgacaa
    19381 accaaggaac tacaatgacc ctgcgtcaga ccattcaatc cgcccccgcc aaggccggcg
    19441 cgatcatcga gaagctttcg aacacctcca accaggcgat caagacccgc gagagcctgt
    19501 tcgccgagct gaccgctgag ctcagcttgt acgtcgacct cgaggaacag cacctcgtgc
    19561 cgctgcttcg caagcatgcc ggcaccaagg cgctcgcccc cgacgccgcc aagggcaaca
    19621 aggagctccg cgcgaagctg gccgagctcg ccgtcgctcc gaaggacgac gatgcgtttc
    19681 tcgagaagct gaacgagctc aagaagggct tccagcagca cgtccgcaac gagcgcaagg
    19741 agctgctgcc ggcggtgctg aaggcgctcg acgatgagga agccaatcag ctcgccgccg
    19801 ccatcgaagg cggcatcgac gaggcggaga atgccaagcg cgagcagaag aagcaggaag
    19861 ccgccgccgc caagcgcgag gccgagcagg ccgaagccgc cgctgccgcc gagcgcgaga
    19921 ccgcgcgggc cgcgaaggcc gccgagcgcc aggcgcgcga agccgccgac aagatggcgg
    19981 agtcgctcga gcgcggcgcc gccgcggcga agcagaccgc ccagtcggtc acggtcgacc
    20041 tgatggagca gacccagcag gccgccaccg gcgctcgcga ggcgatgacc gcctatagcg
    20101 gcacggtcca gaaggctgcc gaggacctgc gcgcggtgtc cgcctcgact aacatcgcgg
    20161 cgaccggcgt ttcgcagttc gtctcggcct ggttcgaatg ggccggcaag gtcgcccgca
    20221 ccaatgccga ggtgtcgcgc cggatgctgc agtcgcgcaa cgtgacgcag ttcgccgaag
    20281 ctcaccagga atatgtgacc agcgcgacgc gcaacctgat cgagggcaac agcgcgttgc
    20341 tcgagatcgc ccagcgcacc tcgaaggagg cgctgcggcc gctcgagggc cagctcgccc
    20401 gctaagccag cgaagtaaat tcaggcgcgc ccgtcgtccc ttgcagggga cagcggcgcg
    20461 cctttttttt ttggcgaagg gcaggggcta tcggggcccg agaggcagcc gcgacacgca
    20521 ccgaagtcct cgtcattcca gcgcaagctg gaatcttgcg ggtcggacct gaaggggaag
    20581 tgagattcca gcttgcgctg gaatgacggg gagcgctgga tgcgcgggtg ccacgcgccc
    20641 gcttgatgtt tgcgcggcga cggtgtttgc tgtcgacatg aggatgacga cacccaaggc
    20701 gctggttgcg ctggtctgtt cggcgctgct gggggcggcg gcgagcgcgg cgcccgcgaa
    20761 ccttttcatc aaaacgggcg agacgatcgt ggtgcgggtc ggggccgacg gggcgatgtc
    20821 ggttgaatcg cgggcagcgg cgccgccgct gtcggggttc gaacaatcgg cgctcgccca
    20881 gatgcaagcg gcgctggcgc aggtgccgcc ggacgcgaag acgctgccgg cgatgccgat
    20941 cctcggcccc aaggatacca aagtccctga catccgcccc aattcggttc ggctgacgtt
    21001 ccgcgcggta gcgggtggaa gcgaggcgat gctctggatc gagaacggat atgacggggc
    21061 gttccgctat cgtgcggtga tgcaccgcgg cgagcggtcg gcgccgacag acgtgtgcca
    21121 ggtgatgccg aaaaaacccg gcgccgagca ttggccgttc cagatcgaaa ggctggagct
    21181 ttcggaggcg cggctggagc cgtggcagga cgggcaaggc atacgctgcc aatagcttcg
    21241 gcgcggaggc tcgacttccg gaccgctcgc ccgctatcgg tcgcggcgtg agcaatcccc
    21301 tctccatcct ccggtcgacc ttcggctttt ccgactttcg cggcgtgcag ccggcggtga
    21361 tcgagcgcgt gatggccggc cagcacacgc tggcggtgat gccgaccggg gcgggcaagt
    21421 cgctctgcta ccaggtgccg gcgctggcgc ggaaggggac ggctttggtg atctcgccgc
    21481 tgatcgcgct gatgcacgac cagatacggt cggcggacgg gttcgggatc aaggcggcgg
    21541 cactgacctc ggccgacgac aaccgccagg agacggtggc gcggcttcgg cggggcgagc
    21601 tcgacctgct ttacgttgcg cccgaacggg cctcggggga cggcttccgg cagctgatcg
    21661 acagcatcaa gctgtcgctg gtcgcgatcg acgaggcgca ttgcgtcagt gaatgggggc
    21721 atgatttccg gcccgactac cggctgctgc gcccgttgct cgaccagttc ggcgaagtgc
    21781 cgcggctcgc gctcaccgcc accgccgacc ggcagacccg cgccgacata ttggtccagc
    21841 tcggcatacc ggaggacggg ctgatcctgg cggggttcga ccggcccaat atccgctacc
    21901 atatccggcc gcgcgacggc gtcggcgcgc aattgaagac gctgctggcg gagcagccgg
    21961 ggccggggat cgtctatgtg ccgagccgcg acaaggccga gcggctggcc gaccaattga
    22021 gcgggccggg ccgccgcgca ctgccctacc atgccgggct cgagccgcag gtgcgggcgc
    22081 gcaaccaggc tgcgttcgtg cgctccgaga atatggtgat gtgcgcgacg atcgcgttcg
    22141 gcatggggat cgacaagccc gacgtgcgct tcgtcgccca tgccggcctg cccaaatcga
    22201 tcgaggctta ttatcaggag accgggaggg gcgggcgcga cggcgatccg gccgaggcct
    22261 ggttgttctg gggcgccgag gatttctcgc gggcgcggcg gcggatcgag accgaagtgc
    22321 cggaggagcg gcggcagggc gagcggcagc ggctaaatgc gctggcggcg gtggtcgaga
    22381 cggccgggtg ccggcgggcg ctgctgctgc gccatttcgg cgagacgccg ccggagcgtt
    22441 gcggcaattg cgacaattgc ctggcgccgc cgagcacggt ggacgcgacc gaggcggcgc
    22501 gcaaattgct gtcggccgcg ttccggaccg agatgcggtt cggaatcgcg catctgatcg
    22561 acgtgctggc gggcaaggag acggacaaga tccacggctt cgggcaccat cggctgtcgg
    22621 tgttcggcat cgctgagggc gacgagctgg cgctgatgaa gccggtggcg cgggcgctga
    22681 tggcccgcga tgcgctgcgc gccgacgaat atggcgggct ttccttcggg ccggcggcca
    22741 agccgatcct caagggcgag gagaagctcg acctggtgct gccgccaaga cgccagagga
    22801 aggcccggcg cggcggagcg gggggcggca atcccgaggg cgacccgctg ttcgaggcgc
    22861 tgcgcgcgtg ccggcgggat ttggccaagg aggcgtcggt gccgccctac gtcgtgttcc
    22921 acgacagtac gctgcgggag atggcggagc tcaggccgac gagcctgcag gcgctgtcgc
    22981 gggtttccgg cgtcggggcg gccaagctcg agcgttatgg caaggcgttc gtcgaggtgg
    23041 tgcgggagca ttcgtcgtga gggggcggct attcccgctc gatcccgata ctctggtctc
    23101 cggccagatc atgccggaag acctgtcagc cctggccgcg gagggcgtga cgatgatcgt
    23161 caacaaccgg cccgacgggg aggagccggg gcagccggcg gcggtggcga tcgaggcggc
    23221 ggcgcgggcg gcggggatcg attatcgctg ggtgccggtg gcgtcgggct tttcgcccga
    23281 tcaggtcgag gcgatgggcg cggcgctgga ggctgcggac ggcaaggtgc ttgccttctg
    23341 ccgctcgggc acgcgctcca ccttcctgtg ggcgctggcg cgaagccggg ccggcgacga
    23401 cggcgacgag ctggtgcgca aggcggcggc ggcgggctac gatctgtcgc cgatccgcgg
    23461 tctccttcag cgctgatcct ccgcgaggat ggtttgggct gccttgaggt cgcgttcgtc
    23521 gaccatcagc cgggcggggg tcatcgcccc gagcccgagg ctggcgaggc cggcgccgaa
    23581 cagcaccgtt tcgatcccct ccgaagcgag ccggccgcgc gcgacctcgg cggcgaccgg
    23641 attctggaac aaggcgagtt cgaccagcgc cattaccagg ggaacacgtc gccggcggcg
    23701 acctcgtcgg ggcggggggt gcggccgagg atcgggttgc ggtgggggaa gcggccgaac
    23761 cgctcgatga cgtcatggtg cttgaccgcg aagccgagct gatgcgggtc gccgagcgcg
    23821 ttgaacaaag ccagcgagcg ctgctggtcg cccgggtcct cgctatgctg gaacggcatg
    23881 tagaggaagc cgcgttccgc cggagtcagt tcccggtcga tgcggcgatc gaccgcgccc
    23941 ctggcgacgg cgagggcgag cggatcggtg gcgaattgct cggcatggcc gcggaacatg
    24001 ttgcgcggga attgatcgaa caggatgacg gccgccaccg cgtcgcgggc gctgccgaga
    24061 taatgatcgg ccggcagccc gcgttgcttt tcccaaagat cgaggaagcg cgcgcggcag
    24121 ctgcggtcga gctcccggtc cgccttccac cattggtcgg gctcgagccc gaaccagaag
    24181 ccgaggacgt cgtcctgcca ggccatcagg cggcgatgct caagcggcgg tgccgccgac
    24241 ggtgaggccc tcgatcagga gggtcggctg gccgacgccc gccgggaccg actggccgcc
    24301 cttgccgcag atgccgatgc cctcgtccag cgccatatcg ttgccgatcg ccgccacttt
    24361 ggtgaggacg gtgggcccgt cgccgatcag ggtcgcgccc ttgatcgggg cgccgaggcg
    24421 gccattctcg atcttatagg cttcggtgca gctgaagacg aacttgcccg aggtgatgtc
    24481 gacctggccg ccgccgaagc ttttggcgta gatgccgttc ttcgcgcggg cgaggatctc
    24541 gccaggatcg tcattgccgc cgagcatgaa ggtgttggtc atgcgcggca tcggcgcatg
    24601 ggcgaagctt tcgcggcggc cgttgccggt cggctcgacg cccatcaggc gggcgttgag
    24661 gcggtcgtgg atatagcctt tgaggatgcc gtcctcgatc aaggtgttga gccgggtcgg
    24721 cgtgccctcg tcgtcgatgg tgagcgagcc gcggcgggcg ccaatcgagc cgtcgtcgac
    24781 gacggtgacg ccgggcgcgg cgacgcggtc gccgatgcgg ccggagaaag ccgagctgcc
    24841 cttgcggttg aagtcgccct cgaggccatg gccgatcgct tcgtggagga ggacgccggg
    24901 ccagccgggg ccgagcacca ccgtcatctc gccggccgga gcggcgatcg aatcgaggtt
    24961 caccagcgcc tgggcgagcg cgatgtcgac cgcgcggttc caggtcgccg gctcgaaaat
    25021 gtcgtcatag aggcgccggc cgccgaggcc gtgataacct gtctcgcggc gcccgttggc
    25081 ctcgaccacg atctggacgt tgagccgcac catcggccgc acgtcatggg cgacgaagcc
    25141 gtcggcacgg acaatgtcga tcacgctcca gctgccggcg aggctgaccg agacctgcgc
    25201 gacgcgcggg tcgcgggcgc gggcggcggc gtcgatctcc tggcagagcg cgaccttgcg
    25261 cgcgaacggc acggccgaga gcggatcggc gtcggtgtaa agcgtggcgt tggtgcggcg
    25321 cggcggcgcg gccttggagg cgctggacgg gtcgagcacg gtcatcgtct cgccggcgcg
    25381 gcggatggcg gcttcgctca attcgttggc atgggcgaag gcggtggtct cgccggccac
    25441 gccgcgcagg ccgaaaccgg cctgagtgtt gaagtcggca ttcttcagcc gcccgtcgtc
    25501 gaagccgaag ctctccgacg cggtatattg aagatagagc tcgccatcgt cgcaggcggc
    25561 gagcgcttcg gcggtgaggc gctgggcggc gtcggggctg agcccgtcgg ggcggtacag
    25621 gaagcggcgg ggatcggcga gcttggtcat agctgtcata tagggacctg ccccgctttc
    25681 cgtcaccctg aacttgtttc gggtcatttc tcagcccgcg gagcggctcg aacatggatg
    25741 ctgaaacgag tccagcatga cgaagaaggc gtcaggctgt ctgctcgccg cggtcggcgg
    25801 ggccgcccct cagcacgaac ctcttgtcgc aatagccgca ttcgacatag ccgctctcgt
    25861 cgatctgcag gaacacgcgc ggatgcccga gcgccgggtg aatctggctc gcgccgtcgc
    25921 aggcgacacg gggcgtgtcg acatagctga tctcgggcac gaacgggtcg tcggtcatgg
    25981 ccgcggcctt agcaaccgcc aatggcgcgc acaatgatcc ctgtcagcgc cgctccggct
    26041 cggctcccgt gccggcaagc tcctgccgca cgcgccgcac catgcgcccg cgttcctgcc
    26101 aggccatcat cgtgccgttg gtcgcgtaga gcgccttcag ataggcgagg tcccatttgg
    26161 tgaggacggc ggcgggggcg atgccctgct ttgggtcgtc gaacaggcgc aggatcgagc
    26221 gcgggccgcc gggcttcgcg ggtggccggg tgctggcgag cgcgcgcatc gcggcataat
    26281 cggcgagttg ggtcaaagtc agcccttcga ccccggcgat gtcgaacacg acgaacgaca
    26341 aagcgaggtc ggatcgcgtc gacttctgca gccgcgagga ttgcatgtgc ggcatcagat
    26401 gcgcctcggg ccgatacccg acgccttcga cgaacggctc ggccacccgg ccgtccgcct
    26461 cgcgccgctc gacgatctgc caggcgcgag ccggggcgcc ggcggcgaac agctcggcgg
    26521 tctcccgccg ccgcagatcg tggaacaggc gcgggcgttt cttacggaac gcagttacga
    26581 agtcccggcc gccgtcggcc acgactacca ccaggttcgg gcggcatttc ggcccggcaa
    26641 gttcgagccc cgccgtcgcc gcgacatcgc ggatgcggtt catgacggcg gcggcatggc
    26701 cttccgggag gccgatcgtc gccggacaga tgcgggcgtg gaagcgggcg agctgattgt
    26761 cgtggtcgcg catgatcgaa tcgatgaaat cggaggcggc gcacttgttg tcgcgcgttc
    26821 cggtgacgac gatctcggga tcgtcctcgg catggcgcgg cggttcggcc gaggccgtcc
    26881 ccgccagcag caacgccgcc tgcacgacct tcgctactat tgaacgcacc gcctgtctcc
    26941 ttcggccgcg atgataaatg gcggacgcga tgcgcgcagc tagccggcat gaccgctgtc
    27001 gaagagtgga cggccctggt cgaaaagcgt caggcgatcg ggagcgaaaa ggcggcgatc
    27061 cgcgccagcc aggcggcgca gatcgccgag agcaataatg cccggcggga gaagccaaga
    27121 agggggcggt taccgaacat ggcggcgatg atgggcgcgg aaccatgact cgcggcttga
    27181 acgatccggt aaatggcggc ttcgccatcc gcgcttccgg tgccttgccc gaaccgctat
    27241 gcggcggcat gtccagcgcc gccgccatct cgatcgacaa tctccagaag acctatgccg
    27301 gcggcaagcg cgcgctcgac ggcgtcagcc tggacgtgcc gcgcggccaa attttcggac
    27361 tgctcgggcc gaacggggcc ggcaagtcga cgatgatcaa catcctcgcg gggctggtaa
    27421 acaagaccgg tggcaccgcg tcgatctggg gcttcgacat cgacgagcat ccgcgcaacg
    27481 ccaaggcctc gatcggcatc gtgccgcagg agatattgtt cgaccccttc ttcacgccgg
    27541 ccgagacgct ggagatgcag gccggcctgt acggcgtccc aaaggcgaaa cgtcggacgg
    27601 cggagctgct gcgcgccgtc catctcgagg acaaggcgcg ggcttattcc cggacgctgt
    27661 cgggcggcat gaagcggcgg ctgctggtcg ccaaggcgat ggtccattcg ccgccgatcc
    27721 tggtgctcga cgagcccacc gccggcgtcg acatcgagct tcgccagcag ctctggaatt
    27781 atgtgcggga gctcaacagc cggggcgtca ccatcgtcct caccacccat tatctggagg
    27841 aagccgagca gctttgcgac cgcatcgcga tcatcaacca cggccggctg atcgccaacg
    27901 aggcgacccg gactttggtc ggcatggcgc tcgaaaaagc ggtggaagtg acggtggacc
    27961 gcgatctaac ccaggcgccg aacgcgccct gcttcgacaa gatcgagctg atcggcgagc
    28021 ggactttggc gatcacctat ctcaagaacc gcgtgaatgc cggcgacgtc atagacgcgc
    28081 tgcagcgcga ggggctgggc atcgtcgacg tctccacccg cgaggctgac ctggaggacg
    28141 tgttcctgag cctgacgcgg gcgtcgaatg cgggatgaat tgctaaattc ctcccctgca
    28201 attgcagagg aggattgagt gaaccatagc ttcgacgtcg tcgtcatcgg ctcgggcgct
    28261 gccgggctga gcgcggcgct gaacctcgcg ccgcacctca aggtggcggt gctggccaag
    28321 ggagcgctgt cgggcggctc gaccgcctgg gctcaaggcg ggatcgccgc cgtgctcgag
    28381 cccggcgaca cgttcgaaag ccatatcgag gacacgatca cggccggcgc ggggctcaac
    28441 caccgccgcg cggtcgaatt cgtggtcgag aacgcgcctg ccgctatcga gcggctggcc
    28501 gagctcggcg tcccgttcaa ccagggcgga tcggaacgct ggcacctcac ccgcgaaggc
    28561 ggccacagcc atcgccgcat cgtccacgtc gccgacgcca ccggctgggc ggtccagcag
    28621 gcgctcgagg aagccgccgc gctccacccc aatatcacgc tcgtgccgga catggtcgcg
    28681 atcgaccttg ccaccggccg ccatgccgag gccagcgcca gcgagaattg cgtgtggggc
    28741 gtctatgcgg tcaaccggcg gaccggcggc gtcgacctgt tcaccggccg cgccaccatc
    28801 ctcgccaccg gcggcgcgag ccgcgcctgg ctctattcga ccaatccgcg cggcagcacc
    28861 ggcgacggca tcgccatggc ctggcgggcg ggctgccggg ttgcgaacat ggagttcaac
    28921 cagttccacc cgacctgcct ccacaatgtg gaggtcaaga atttcctgat caccgaggcg
    28981 atgcgcggcg agggcggccg gcttaccatc cccggctccg gccatcgctt catgccggat
    29041 ttcgacccgc gcgcggagct cgccccccgc gacatcgtcg cccgcgccat cgaccatgag
    29101 ctgaagcggc tcggcatcga ttatgtccat ctcgacatca gccaccgcga tccggatttc
    29161 gtccgctcgc acttcccgac catcgccgag cgacttgccc agcccgacat cgggatcgac
    29221 atcacccggg agccgatccc ggtcgttccg gccgcccatt atacgtgcgg cggggtggtc
    29281 gtggacctcg acggcaagac cgacctcgat gggctttacg ccgccggcga agtgaccatg
    29341 tcgggcctgc acggggccaa ccggctggct tcgaactcgg tgctcgaatg cctcgtcttc
    29401 gggctcgccg ccgcccaccg catcaaccag ggctggagcg agctgccgcc gccgccgccg
    29461 atcaggccct gggacgagag ccgcgtcacc cattcggacg aggaggtggt cgtccatcat
    29521 aattggcgag agatacggcg cttcatgtgg gattatgtcg ggatcgtccg caccaccaag
    29581 cggctcgagc gagcgctcaa ccgggtcgag ctgctgcgcc aggagaccga ggattattac
    29641 ggccatttcc gggtcacgcc cgatctggtc gaattgcgca atttggtcga agtgtcgggc
    29701 ctgatcgttc gctcggccct cgcccgcaag gagagccgag ggctccatta tacgcttg
//
LOCUS       DASVRK010000145         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_89777, whole genome shotgun sequence.
ACCESSION   DASVRK010000145 DASVRK010000000
VERSION     DASVRK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89777"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VF589_05845"
     CDS             complement(<1..591)
                     /locus_tag="VF589_05845"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX8257134.1"
                     /translation="MKLMSGNSNLPLVKAIASYLELPVTDASVRRFADEEIFVEINEN
                     VRGEDVFVVQSTAYPANDNLMELLICIDALRRASARRITAVLPYFGYARQDRKPGPRT
                     PISAKLVANLITVAGANRVLSIDLHAGQIQGFFDIPTDNLYASPVMSADIQARFSGEA
                     ITVVSPDVGGVVRARGLAKRLDNAPLAIVDKRRERAG"
     gene            complement(744..>2734)
                     /locus_tag="VF589_05850"
     CDS             complement(744..>2734)
                     /locus_tag="VF589_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257135.1"
                     /translation="IVGAPGGEFGGSNAGEAYVIFGKSSGLGDIDLTSLAAEDGFVIG
                     GLGAASFVGRSVASAGDVNGDGVDDIVLGVAYGDAQGDNSGQAYILFGKSAGLGDVDL
                     ATLDPDEGFLIEGGLAFGKAGWSAASAGDVNGDGFDDVIVGQPNLPSETSGNAGAAYL
                     VFGKADGFGTLNLAAPDPDAVVSFFGASGGDQVGWSVAAAGDVNADGFADFILGSSTN
                     TGAGAGKSYLIYGKAEPFDSIHLAGLRAADGFLIRGVTSNDQAGRSVSSAGDVNGDGF
                     ADLLLGAQRGDDGGVDAGQAYVIFGAAPTAAVTRVGSAAAQTIRGGTGDDLLDGRDGG
                     DLLFGAGGDDRLVGGAGDDGLSGDAGDDDLSGGSGNDSLNGGFDNDRLAGGSGNDLVD
                     GGAGADLLIGGSGNDRYNVDRMGDRIVEEEGSGADRLFARANYTLRDGVDIETISTIA
                     NSGTATISLTGNEIANSITGNAGVNALAGGGGGDQLFGLAGNDRLSGGAGNDLATGGA
                     GTDRFLFDTALSATANVDRLLDFSPTDDMIALDRAIFGGIPSGVLTAAAFRAGAAAQD
                     DSDRIVYDAATGNIYYDADGLNGAAQILFAQVDAGTPLTRVDFLGFDSGSAAAARLPQ
                     SAFAPAPEHGQGAGTASRSLHMEAVPLSGPEWMDVQIA"
BASE COUNT          443 a          947 c          924 g          420 t
ORIGIN      
        1 gccggcgcgc tcccgccttt tgtcgacgat ggcgagcggc gcgttgtcga ggcgcttggc
       61 gagcccgcgg gcgcggacca cgccgccgac gtccggcgag acgacggtga tcgcctcccc
      121 tgagaaccgc gcctggatgt cggccgacat caccggcgag gcgtaaagat tgtcggtcgg
      181 gatatcgaag aagccctgga tctggccggc gtggagatcg atcgacagca cgcgattggc
      241 gccggcgacg gtgatcaggt tggcgaccag cttggccgag atcggcgtgc gcggccccgg
      301 cttccggtcc tggcgggcat agccgaaata agggagcacc gccgtgatcc gcctggccga
      361 ggcgcgccgc aacgcgtcga tgcagatcag cagctccatc agattgtcgt tggccggata
      421 ggcggtggac tggaccacga acacgtcctc gccgcggaca ttctcgttga tctcgacgaa
      481 aatttcctcg tcggcgaagc ggcggacgct ggcgtcggtg accggcagct ccagatagct
      541 ggcaatcgcc ttcaccagcg gaaggttgga attgcccgac atcaatttca tcgatccgtc
      601 cccaaccccg tctagccgat agcgaaaccc cctctaagcg ccgacggagc gaaggcaagg
      661 gcgagcttgg ccccggcagg atcggaggtt catccccttc gaagctgtag ccctgccatc
      721 ggcgctttcg accattgcag cggtcaggcg atctgcacgt ccatccattc gggaccggag
      781 agcggaacag cctccatgtg aaggctccgg ctcgccgtgc ccgcgccctg cccgtgctcc
      841 ggcgcaggcg cgaaggcgga ttgcggcaat cgggcggcgg ccgcgctgcc gctatcgaag
      901 ccgaggaaat cgacccgcgt cagcggcgtg ccggcgtcga cctgggcgaa caatatctgc
      961 gccgcgccgt tgaggccgtc ggcatcgtag tagatgttgc cggtggccgc gtcgtagacg
     1021 atccggtcgc tgtcgtcctg cgcggcggcg ccggcccgga aggcagcggc ggtgaggacg
     1081 ccgctcggga tgccgccgaa gatggcgcgg tcgagcgcga tcatatcgtc ggtcggcgaa
     1141 aaatcgagca gccggtcgac gttggctgtt gcgctgaggg cggtgtcgaa caggaaccgg
     1201 tcggtccccg ccccgccggt cgccaaatcg ttgccggccc cgccgctcag ccggtcgttg
     1261 ccggccagcc cgaacaattg gtcgccgccg ccgccgccgg cgagcgcgtt gacaccggca
     1321 ttgccggtga tcgaattggc gatctcattg ccggtgaggc tgatcgtcgc ggtgccgcta
     1381 ttggcgatgg tgctgatcgt ctcgatgtcg acgccgtccc gcaacgtgta attggcgcgc
     1441 gcgaacagcc ggtcggcgcc gctcccctcc tcctcgacga tccgatcgcc catccgatcg
     1501 acattatagc ggtcgttgcc cgaaccgccg atcagcagat cggcgccggc gccgccgtcg
     1561 accaggtcgt tgcccgagcc gcccgccagc cgatcattgt cgaagccgcc attgaggctg
     1621 tcattgccgg agccgccgct cagatcgtcg tcgccggcat cgccgctgag gccatcatcg
     1681 ccggcgccgc cgaccagccg gtcgtcgccg cccgcgccga acagcagatc gccgccgtcg
     1741 cgcccgtcga gcagatcgtc gccggtgccg ccgcggatgg tctgggcggc cgccgagccg
     1801 acgcgcgtca ccgccgcggt cggcgcggcg ccgaagatga cataggcctg gccggcatcg
     1861 acgccgccgt catcgcctct ctgggcaccg aggaggagat cggcgaaacc gtcgccgtta
     1921 acgtcgcccg cggacgacac gcttcgcccc gcctggtcgt tggaggtcac gccccggatg
     1981 agaaagccgt cggcggcgcg cagcccggcc aggtggatcg agtcgaacgg ctcggccttg
     2041 ccgtagatca aataggactt gccggcgccc gcgccggtat tggtgctcga cccaaggatg
     2101 aaatcggcga agccgtcggc attgacgtcg cccgcggcgg cgacgctcca gccgacctga
     2161 tcgccgcccg acgcgccaaa gaaggagacg acggcgtcgg gatccggcgc ggcgagattc
     2221 agggtcccga agccgtccgc tttgccgaac accaggtaag cggcgcccgc attgcccgac
     2281 gtctccgacg gcagattggg ctggccgacg atcacatcgt cgaaaccgtc gccgttgacg
     2341 tccccggcgg aggcggcgct ccagcctgcc tttccgaaag caaggccgcc ctcgataagg
     2401 aacccttcgt cgggatcgag ggtagcgagg tcgacgtccc cgagcccggc cgacttgccg
     2461 aagagaatat aagcctgtcc ggaattgtcg ccctgagcgt cgccataagc cactccgagc
     2521 acgatatcgt cgacgccgtc gccattgacg tcgccggcgg aggcaacgct gcggccgacg
     2581 aagctggcgg cgcccaatcc tccgatgacg aacccgtctt cggcggcgag gctcgtcagg
     2641 tcgatgtcgc cgaggccgct gctcttgccg aagatcacat aggcctcgcc cgcattgctg
     2701 ccgccaaact cgccgcccgg ggctccgacg atca
//
LOCUS       DASVRK010000146        15007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_401516, whole genome shotgun sequence.
ACCESSION   DASVRK010000146 DASVRK010000000
VERSION     DASVRK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401516"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..2369)
                     /locus_tag="VF589_05855"
     CDS             complement(213..2369)
                     /locus_tag="VF589_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8257136.1"
                     /translation="MIRRRFNEAEELGFPIRAVALGVFAMVVIALASTATAWLVGQRI
                     RDIGGNQITVLTQAERLQRHSDLLQVYARIAVASGDAEFQQRYGTEEAALRKDLDQLK
                     GAIRLAENRVTFDRLERTQRALAEIEYQAMEMAVAGRRPAARALLAGNDYLQLTRNYR
                     NSADDIADRSRAYVAATRDETRRYLSINLGTSVMALLLIALAWVILVRPARSWGLQLA
                     EERARAETAAHAKGDFLAMMSHEIRTPLNSIIGFSDLLRDDPELTESQRHKVDLIQSA
                     GMMLLTVVNDVLDFSKFEAGRIELLPEPFAVETLVDNSVSIVRDSAEAKGLELRLVVD
                     PKMSRYHLGDEGRLRQILLNLLNNAIKFTAAGLVSLAVRKEAEVGEAERLLFSVTDTG
                     TGIKEDRQQSLFEPFSQGDASVTRTYGGTGLGLSISKRLVELMDGEIGVMSTEGQGSY
                     FWFRITLPRAARLEPADDGAAETTGHAGARILIAEDLPMNRELAGAILSRAGHEVDMA
                     EDGEQALEKARSGDYDLILMDIQMPKMDGLSATRAIRALAGARGQVPIIALTANALPE
                     QVREFLAAGMSGHVAKPIRQPELHRVIRAILAESAAAPAAPAPPPALAFDEDTFAEVR
                     EMLPADRLRGHLERLDRDLAALTDGSLDDQAARAMVHKLISQTGMIGFMHFSKLAADA
                     EGGIDGDGSEAALAGLRRAAQPVRQRLAALIAEIGA"
     gene            complement(2366..3784)
                     /locus_tag="VF589_05860"
     CDS             complement(2366..3784)
                     /locus_tag="VF589_05860"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8257137.1"
                     /translation="MTGGYDIGVAAAELIAWVVIGTGLAQTAFYIVQLGYAGLALGSR
                     PPIPKASILWRRYADQAPAIAVLAPAYNEEVTIIESVNSLLSLQYPDFEVIVINDGSR
                     DTTLQRMIEEYGLAPVRRFHDNAVSHRPIRGLYATPRLPRLIVVDKENGGKSDALNAG
                     INVARAPIFCSIDADSLLEPDALLRAVRPFIEDPDRTVAVGGTIRIANGSRIDAGRVT
                     AIRLPRNFLALVQVVEYLRAFLMARLALGRMQALTVISGAFGLFRRQPAVEVGGYSHG
                     TVGEDMELVIKLHAHFRKAGRPYRIDFIPEPVCWTEVPESLAVLGRQRARWQRGALET
                     FFRHKYMLFNPRYGRVGFVGFGQVLVVDVIGPPVEALGYLLVPLLWGLGLLSLDFLLA
                     FLAVTFTFGIFVSVATLILEEVELRRFPRAGDLCILTLVAIAENFGYRQIGNFWRVRG
                     WWQYLRGQQGWGDMTRKGFQRA"
     gene            complement(3781..4821)
                     /locus_tag="VF589_05865"
     CDS             complement(3781..4821)
                     /locus_tag="VF589_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8257138.1"
                     /translation="MSALQALWLLSLILALLALIIMAGLLVGRAFRTWRGTRREAVRR
                     RLIPLLLGGGGGRGALASRERQDLLATLAVELIQMVRGREREAFIENATRLGVPERLR
                     HHLGSGSARTRQQAAEALSEFGDDRSISRLEAALDDPNPDVRLTAALALAPIGRLPSA
                     RRLVDQLGIGTTEHSLLVVSLFREIASSRSEELKALLYDPTVPSGAKAAAIDALSASP
                     DYSLVPLVSQLALRLPAEDPALPRYLRALAGFGHPAGAPAIERGLASEAWAARAAAAH
                     AAGKIGLAETAPRLAELLDDVNWWVRFRAGEALSHLGPEGQSLLVETARSGPDRARTA
                     AALTLAERSGPE"
     gene            complement(4818..5825)
                     /locus_tag="VF589_05870"
     CDS             complement(4818..5825)
                     /locus_tag="VF589_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEX8257139.1"
                     /translation="MKAPAAAAFLLLLSTAAEAQQSAYERGVAARLAGDAEAAVAALS
                     EAVAAEPGNADAHLQLGLALLQAGRLDEAEASLRRTLALAPDYEDAHAALARLAQRRG
                     AAPEYRTRMDIDGSYSALEGDRPDWKEVSLQVRHRVGDRSAIGAGAEYARRFGRSDLY
                     LEGRLEHRFSDAATAYASLGGTPEADFRPEWQVGAGGSLRLGGRANATVLTLDARAAE
                     YPAGRIYTVNPGVEQYLAGGRVWLTGRWINVFDEDGDHVSGWLGRGDVMATDRLRLFA
                     GASDAPDLSEGVVTDLFSLFGGVSYDINARTTLRLSLAHEDRERGGDRLQAGAGLGFR
                     F"
     gene            complement(5822..6211)
                     /locus_tag="VF589_05875"
     CDS             complement(5822..6211)
                     /locus_tag="VF589_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8257140.1"
                     /translation="MAAGERKRILICDDDPLLLDLVRHRLSSRGYDVEVAEDGAEALS
                     SIEMSPPDAVVLDAMMPVVDGYELLRRLRARPETAAIPVIMLTARRQEEDVLSALQLG
                     ADDYMVKPFIPEELVMRLGRLLDGESR"
     gene            6799..9120
                     /locus_tag="VF589_05880"
     CDS             6799..9120
                     /locus_tag="VF589_05880"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8257141.1"
                     /translation="MCWICNPLVKNALATDHWASVMGIEAAHFEALSKGETALGPDGI
                     GDKGDAVAPVALITTDNVGDDIGSAASVEVNGDHIISSIDTVGDQDFFAVTLEAGKLY
                     NIGQYAAVAGPNGVPLSDAFIELYDANGNLIVSADGGGPNTPSGLDALLSFEAQETGT
                     YYVNARSFDNVPEDGTNGDVVGDYELFVDEVDPNDPNVYRPYYETDSPLYSLDWGTQV
                     NRVHQTVRNPDGEEGPRPTGNEQEAPPENAYNITDKNVITIYFAREGDVFASEDPTNP
                     GLPPVLVSAGTKDWEKEVVNTALDQIEEVADVVYVEVDNREEADFFFTTYAGTPGPGV
                     SLLGSMAPPDYPDEGLAQFNSGDYRWTEKNLQQGGFSYVTLVHEFGHGMGMAHPHDNG
                     GRSGIMNGVEEESTFNYSTGDFNLNQGINTMMSYEDGWQMSPYGNAPTDAGYGYLGGP
                     MAFDIAVLQDKYGVNEDHATGNNIYRMKDVNAAGTFYDSIWDAGGHDQIIYDGERNSK
                     VDLRDATLEYEIGGGGFISYAHGIFGGYTIANSVTIEDARTGSGNDLINGNEVGNRLI
                     SNAGHDTVRGLGGQDAIFGGVGNDKLFGGSQNDNMSGGEGLDFVLGQTGNDRVSADAG
                     NDVARGGDGNDRVAGGEGDDRINGNLGNDVVIGGAGIDMIAGGGDADIFVFGEGDSGS
                     SRSTADHILDFSSDNGDSINLAQIDAIAGTAANDSFTFIGKGAFSGTAGELRYFVSDG
                     DAIVSADTDGDKLADFVLVMDGRSQLAATDFLV"
     gene            complement(9216..10130)
                     /locus_tag="VF589_05885"
     CDS             complement(9216..10130)
                     /locus_tag="VF589_05885"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8257142.1"
                     /translation="MAADHIGELKRRAAAGDASALFELGALSLAGEAVPRDLAVAREM
                     LRRAAVAGRKDAAVIYANFVASGVGAAADWPRGLALLRELARVNPRSARELEVIGAME
                     LGPEGEPAGTWRRERLSECPRVELAREFFSPLECDYVTAAAEPMLAPSVVVDPATGRQ
                     IPNPGRTSDGMGFTWPLENPAVHALNRRIARLTQTDVAQGEPLQVLRYRGGQQYRPHF
                     DAIPGFANQRILTVLVYLNEGYEGGETWFETPQLKVKGGRGDALVFRNVDDEGRPDKD
                     AGHAGLPVTQGTKLICSRWIRAAPYTPA"
     gene            10343..11776
                     /locus_tag="VF589_05890"
     CDS             10343..11776
                     /locus_tag="VF589_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8257143.1"
                     /translation="MLVSLFALSTALAQAQAPSGSPLAPPPTAIPVSPQVQDLPQPTR
                     DPLRIDRNADPVLGLARNSARPTEFVGLIQQAVERHPAVDEAAAGIEEAEAATEEARA
                     GRYPTVDLSLNSQRSIIRNFEDNDPNNIVERARARGRSDLTLTVQQTLFDAGATEKRI
                     AAAGARLRAASFQTEAQADQIALRAIAAWYDVFAYRALVELGGAFMENQQELRSAIEI
                     RITQGVSAPGDLPRVESYIASADADLARYRRQLANAEARFEEMFGVPAPPGLGRAPII
                     SRPSTTKDAAQFLARSAPSVEAAEANARASRQEARAERASTLPTITAGIDAGKYGLVD
                     QNDYDVRGRVTLRHRFFGGVDARADQAEARANAAEARASRVRNEAEREAAIAWSDVRA
                     LEQQLAALENSYIASRQSRDVLAERFRVARGTLFDLLESESGYFNVAATYIRTITELD
                     AARYVLLSRTGQLLPVLQIEPARLEVQ"
     gene            11773..13506
                     /locus_tag="VF589_05895"
     CDS             11773..13506
                     /locus_tag="VF589_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX8257144.1"
                     /translation="MSDDTIVEIPKQRFAPWLMEPMKRNKGIYIKVGLAAALINMFAL
                     LTSIFTMVVYDRVLPNNATSSLIALSIGLAIVIVFDFALKTLRAYFVDVAGARIDREV
                     GESVFAQLLAIRLDQKKGSTGALAGVIRELETLRDFFASATLTAVVDVPFIFVTLGVI
                     ALIGGWVVTVPLIMIPLVVLVGLITQPAMDRLAAQSMGQGLLKQTVLVETIGGLETVK
                     TSGAAPILTERWLGAVEEHSSTSMRQRLVANIGVTIAGSAQTISYAGVVIVGVGMIAE
                     NTLTMGGLIACSILSGRAVAPLGQIATLLSRMTATRTAYRQLNGLMSTRPEGPEGEPL
                     VLTQTSGRIEFRNVHFRYPGAPEKALEGVTFTLQPGEHVALLGRVGSGKSTIARLLLG
                     LYEPEEGVILLDGTDIRQLDPLDLRRKVGSAMQESILLSGSVRENIVLGRHGIGDDEM
                     IQAAELSGTHQFMGQIANGYDRRLADRGEGLSGGQRQSIAIARALATKPNVLVFDEPT
                     SAMDQQTESGLLDRLDRELKDRTFLVITHRTPLLRLVSRVIILDRGKIIADGPRDQIL
                     AKLNRPKVAAV"
     gene            13503..14807
                     /locus_tag="VF589_05900"
     CDS             13503..14807
                     /locus_tag="VF589_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX8257145.1"
                     /translation="MNREVALEDYIKRVRPAKASNILLWVIAGFVVVFVVWAALTELD
                     RTVRGQGRIIPSSQLQVVSNLEGGVVEAILVKTGDMVQRGAALVRLDQTSTGAELGSN
                     QAQFDALRVRIARLEAEVAGRSPNFPPAVNSSLAEQVAIERSLHASRMADLGSLLAAA
                     NARVVQAQRSVTEAQASLAARQSAAGAARTEANLIRPLVERGIEPRLSLIQAENQAAV
                     SAAEAAAASAAVNRAQSSVAEAQATLAQQRQDWRARASQELASAQAEMVALRRSLPAL
                     SARVERTVVRSPLAGRVNRVLVTTVGGTIAPGGPIAEIVPSEDALLVEVQVRPADIAW
                     VRMDQNAKINITAYDSSVYGALDGKVVAISPDATLNERTGETFYTVRVATTDELFDKS
                     GKELEIGSGMVADISLLGDKRTVLSYILSPFTRLSETALREE"
BASE COUNT         2466 a         5203 c         5018 g         2320 t
ORIGIN      
        1 gcccctccac cacccttcgg gtggtccccc tcccccggaa aaccgaggga ggttacaagt
       61 cctccccggc ctttgccggg gagggggacc ggcggagccg gtggaggggc tgcgcgaagc
      121 gcattcctgc gatgagcatc agcaccggct cgatgtcgtc gacaatctgt gccgtcaccg
      181 cgtccttgag cgcgcgcggg gcggcggtct cgtcaggcgc cgatttccgc gatcagcgcc
      241 gccagccgct gccgcaccgg ctgggccgcc ctgcggaggc cggcgagcgc cgcttcgctt
      301 ccgtcgccgt cgatcccgcc ctccgcatcc gccgcgagct tcgagaagtg catgaagccg
      361 atcatcccgg tctgggagat gagcttgtga accatcgccc gcgcggcctg gtcgtccagg
      421 ctcccgtcgg tcagcgccgc gaggtcgcgg tcgagacgct cgagatgtcc gcggaggcga
      481 tcggccggaa gcatctcgcg gacctcggca aaggtgtcct cgtcgaacgc gagcgccggc
      541 ggcggcgccg gggccgccgg cgcggccgcc gactcggcga gaattgcccg gatcaccctg
      601 tgcagctcgg gctgccggat cggcttggcg acatggccgc tcatgcccgc ggccaggaat
      661 tcgcggacct gctccggcaa cgcgttcgcc gtcagcgcga tgatcggcac ttgcccgcgc
      721 gcgccggcca gcgcgcggat ggcgcgggtg gcgctgagac cgtccatctt cggcatctgg
      781 atgtccatga ggatgaggtc gtaatcgccc gacctcgctt tctcgagcgc ctgctcgcca
      841 tcctcggcca tatcgacctc gtgcccggcg cggctcagga tcgcgcccgc cagctcgcgg
      901 ttcatcggca ggtcttcggc gatcaggatg cgggcgcccg catggccggt ggtctcggcc
      961 gctccgtcgt cggcgggctc cagccgcgcc gcccgcggca aggtgatgcg gaaccagaaa
     1021 taggagccct ggccttcggt gctcatgacg ccgatctcgc cgtccatcag ctcgaccagg
     1081 cgcttggata tgctgagccc gaggccggtg ccgccatagg tccgggtgac cgaagcgtcg
     1141 ccctgcgaga acggctcgaa caggctctgc tgccggtcct ccttgatgcc ggtgccggtg
     1201 tcggtgacgc tgaacagcag ccgctcggcc tccccgacct cggcctcctt gcgcaccgcc
     1261 agcgacacca aaccggccgc cgtgaacttg atggcgttgt tgagcaggtt gagcaggatc
     1321 tggcggagcc gtccttcgtc gccgagatgg tatcgcgaca ttttgggatc gacgacaagc
     1381 ctgagctcca gccccttcgc ctcggcgctg tcgcgaacga tcgagaccga attgtcgacc
     1441 agcgtctcga ccgcgaacgg ctcgggcagg agctcgatcc gcccggcctc gaacttcgag
     1501 aaatcgagca cgtcgttgac cacggtcagc agcatcatgc ccgcgctctg gatgagatcg
     1561 accttgtggc gctggctttc ggtaagctcg ggatcgtcgc gcagcaggtc cgaaaagccg
     1621 atgatgctgt tgagcggcgt gcggatctcg tggctcatca tggcgaggaa atcgcccttg
     1681 gcgtgggcgg cggtttcggc gcgggcgcgc tcctcggcca gctggaggcc ccaactgcgc
     1741 gcgggccgga ccaggatcac ccaggcgagc gcgatcagca gcagcgccat cacgctggtg
     1801 ccgagattga tgctgagata gcgccgggtc tcgtcgcggg tcgcggcgac ataggcgcgc
     1861 gaacggtcgg caatgtcgtc cgccgaattg cggtagttgc gggtcagctg aagatagtcg
     1921 ttgccggcga gcagcgcgcg ggcggccggg cggcggccgg ccaccgccat ctccatggct
     1981 tgatattcga tctcggcgag cgcgcgctgg gtgcgctcca gccggtcgaa cgtgacccgg
     2041 ttctcggcga ggcggatcgc gcccttgagc tggtcgaggt ccttgcgcag cgccgcctcc
     2101 tcggtgccgt agcgctgctg gaattcggca tcgccgctgg cgaccgcaat ccgagcatag
     2161 acctgcagca ggtcgctgtg gcgctgcagc cgctcggcct gggtgaggac ggtgatctgg
     2221 ttgccgccaa tgtcgcggat gcgctggccg accagccagg cggtggcggt cgaggcgagc
     2281 gcgatcacca ccatcgcgaa gacgccgagc gcgaccgccc ggatcggaaa gccgagctct
     2341 tcggcctcgt tgaagcggcg ccggatcatg cccgctggaa ccccttgcgc gtcatatcgc
     2401 cccagccctg ctggccgcgc agatactgcc accagccgcg gacgcgccag aaattgccga
     2461 tctgccggta tccgaaattc tcggcgatcg cgaccaaagt caggatgcac aggtcgccgg
     2521 cgcgcgggaa gcggcgaagc tcgacctcct cgaggatcaa ggtcgccacg ctgacgaaga
     2581 tgccgaaggt gaaggtgacg gccaggaagg cgagcaggaa atcgagcgag agcagcccca
     2641 gaccccacag caagggcacg agcagatagc cgagcgcttc gaccggaggg ccgatcacgt
     2701 cgaccaccag cacctggccg aagccgacga agccgacgcg cccgtagcgc gggttgaaaa
     2761 gcatgtattt gtgcctgaag aaagtctcca gcgccccgcg ctgccagcgc gcgcgctggc
     2821 ggccgagcac cgccaggctt tccggcactt cggtccagca gaccggctcc gggatgaagt
     2881 cgatccggta tggcctcccc gcctttcgga aatgcgcatg gagcttgatg acgagctcca
     2941 tatcctcgcc gacggtgccg tggctgtagc cgccgacctc gaccgccggc tggcgccgga
     3001 acaggccgaa cgcgcccgag atcacggtca gcgcctgcat ccgcccgagc gcgagccgcg
     3061 ccatcaggaa ggcgcgcaga tattcgacga cctgaacgag cgcgaggaag ttgcgcggca
     3121 ggcggatagc ggtcactcgt cccgcatcga tgcggctgcc gttggcgatg cggatggtgc
     3181 cgcccaccgc caccgtccga tcgggatcct cgatgaaggg ccggacggcg cgcagcaaag
     3241 cgtcgggctc gagtagcgaa tcggcgtcga tcgagcagaa gatcggcgcg cgcgcgacgt
     3301 tgatgccggc gttgagcgcg tcggacttgc cgccattctc cttgtccacg acgatcagcc
     3361 gcggcagccg cggcgttgca tacaggcctc ggatcggacg gtgagagacc gcattgtcgt
     3421 ggaagcgccg caccggcgcg aggccatatt cctcgatcat ccgctgcagc gtggtatcgc
     3481 gcgagccgtc gttgatcacg atcacttcga aatcgggata ttggagcgac aatagcgagt
     3541 tgacgctctc gatgatggtc acttcctcat tgtaagccgg cgcgagcacg gcgatggcgg
     3601 gggcctggtc ggcgtagcgg cgccacagga tcgaagcctt cgggatcggc ggccgcgagc
     3661 cgagcgccaa gccggcatag ccgagctgga cgatgtaaaa ggcggtttgc gcgagccccg
     3721 tcccgatcac cacccaggcg atgagctcgg ccgcggccac cccgatatca tagccgccgg
     3781 tcattcgggg ccgctccgtt cggccagggt cagcgccgcc gcggtgcgcg ctcgatcggg
     3841 gccgctgcgc gcggtttcga cgagcaggct ttgcccttcg gggccgagat ggctcagcgc
     3901 ttcgccggcc cggaaccgca cccaccagtt tacgtcgtcg agtagctcgg ccaggcgggg
     3961 cgcggtttcg gcgaggccga tctttcccgc cgcatgcgcc gccgccgcgc gcgccgccca
     4021 ggcttcgctc gccaggccgc gctcgatcgc gggcgccccg gccggatggc cgaagccggc
     4081 gagcgcgcgc aggtagcgcg gtagagcggg gtcttcggcg ggcaggcgca aggcgagttg
     4141 cgagacgagc ggcacgaggc tgtaatcggg cgaggccgag agcgcgtcga tcgccgccgc
     4201 cttcgcgccg ctcggcaccg tggggtcgta gagcaacgcc ttcagctcct ccgagcggct
     4261 gctggcgatc tcgcggaaca gcgaaacgac cagcagcgaa tgctcggtcg tgccgatgcc
     4321 gagctggtcg accagccgcc gcgcgctcgg cagccgccca atcggcgcga gcgcgagcgc
     4381 ggcggtgagg cggacgtcgg gattgggatc gtcgagcgcg gcttcgagcc gggagatcga
     4441 gcggtcgtcg ccgaactcgg aaagcgcttc ggcggcctgc tggcgggtac gcgccgaacc
     4501 gctgccgaga tggtggcgga ggcgctccgg gacgccgagg cgggtggcat tctcgatgaa
     4561 agcctcgcgc tcgcggccgc ggaccatctg gatgagctcg accgccaggg tcgcgagcag
     4621 gtcctgccgc tcgcgcgagg cgagcgcgcc gcggccgccg ccgccgccga gcagtagcgg
     4681 gatcagccgg cgccgcacgg cctcgcgccg agtgccgcgc caggtccgga aggcgcggcc
     4741 gacgagcaga ccggccatga tgatgagggc gagcaaggcg aggatcagcg aaagcagcca
     4801 gagagcctgg agcgcgctca aaagcggaag cccagcccgg cgccggcctg cagccggtcc
     4861 ccgccccgct cgcgatcctc gtgcgccagc gacaggcgaa gcgtcgtgcg cgcatttata
     4921 tcgtaggaga cgccgccgaa gaggctgaac agatcggtga cgacgccctc cgaaagatcg
     4981 ggagcgtcgg aggcgccggc gaacagccgc aggcggtcgg tggccatgac gtcgccgcgg
     5041 ccgagccagc cggagacatg gtcgccatct tcgtcgaaga cgttgatcca gcggccggtc
     5101 agccacacgc gcccaccggc cagatactgc tccacgcccg gattgacggt gtagatccgc
     5161 cctgcgggat attcggcggc gcgggcgtcg agggtgagga ccgtagcgtt tgcgcgtccg
     5221 ccgagccgga gtgagccgcc ggcgccgacc tgccattccg gccggaaatc ggcctcgggc
     5281 gtgccgccga gcgaagcata ggcggtggcg gcgtcggaaa aacggtgttc cagacgccct
     5341 tccagataga ggtcgctgcg gccgaagcgg cgtgcatatt cggcgccggc gccgatagcg
     5401 cttctatcgc ccacgcggtg gcggacctgg agcgagactt ccttccagtc gggccggtcg
     5461 ccttcgagag cgctgtagct gccgtcaatg tccatccggg tccgatattc gggcgcggcg
     5521 ccgcggcgtt gggcgaggcg ggcgagcgcg gcgtgggcat cctcatagtc cggcgcgagt
     5581 gcgagcgtgc ggcggagcga ggcctcggct tcgtccagcc gccccgcctg gagcaacgcc
     5641 aggccgagct ggaggtgcgc gtcggcattg cccggctccg ccgccacggc ttcggacagt
     5701 gccgccaccg ccgcctccgc gtcgccggcc agccgggcgg cgacaccccg ctcataggca
     5761 gattgctgcg cctcggccgc cgtcgacagg aggagcagga aggccgccgc ggccggcgcc
     5821 ttcaccgcga ctcgccgtcg agcaggcgcc cgaggcgcat cacgagctcc tcgggaatga
     5881 acggcttcac catataatcg tcggcgccga gctggagggc ggagagcaca tcctcctcct
     5941 ggcgccgcgc cgtcagcatg atcaccggaa tagcggccgt ctcgggccgc gcgcgaagcc
     6001 gcctcagcaa ttcgtagccg tctaccaccg gcatcatcgc gtcgaggacc acggcatcgg
     6061 gcggcgacat ctcgatgctg gacagcgcct ccgccccgtc ttccgcgacc tccacgtcat
     6121 agccgcggct gctcagccga tggcgaacga ggtccagcaa caacgggtcg tcgtcgcaaa
     6181 tcaggatacg cttacgctcg ccggcagcca caatacgcct actcgaaact ggaatagcgg
     6241 accgttcccg gcccggggtg gaatcaatcg gccaacgtag atcaagttcc gcgccaaggc
     6301 tctcccacgc caggataagc cggctctgtt gcgttggcgc aacgttcccc caggctcgcc
     6361 gcgccgtccc cgatcatccg caattggcga cgaaacgcta ccttggaacg gcaggccgag
     6421 caacgaaccc ggaatttgcg ccaccaacgc aggaacttag ttgatgaacg cccgtttaac
     6481 catcgcatgc cggacgcgcc ggctgcatct tatgggggca gcatttgcat cgctatttcg
     6541 cggcacagcg tggtttgatc gaccttcggg cgtcacatca tcgcgggccc gcgcgccagc
     6601 ggttcaccgc attccccttc tcccatcccc ctaacagcct gctggagatg cgggtcggcc
     6661 gagagcttcg cctctagccg aacttccatc cttcacggtc gctgagctcc gccggaactt
     6721 ccggaggctc gcttcgcgcc cgtccggcag gccggctgcc gcataaacga aaacaatcgt
     6781 atcttaggag agaacaagat gtgttggata tgcaatccgt tggtgaagaa cgcgctcgca
     6841 accgatcact gggccagtgt catggggatc gaggcggcgc attttgaggc gctgtcaaag
     6901 ggcgagactg cgcttgggcc cgatggcatc ggggacaaag gcgacgccgt tgctccggtc
     6961 gcgctgatca ccaccgacaa tgtcggcgac gacatcggca gcgcggccag cgttgaagtc
     7021 aatggcgacc acatcatcag ctcgatcgac acggtcggcg accaggattt cttcgcagtc
     7081 acgctcgagg ccggcaagct ctacaatatc ggccaatatg cggccgtcgc cggtcccaac
     7141 ggcgtgcctc tgtccgacgc attcatcgag ctttacgatg cgaacggcaa tttgatcgtg
     7201 tccgccgacg gcgggggccc caacacgcca tcaggcctcg atgcgctgct ctccttcgaa
     7261 gcgcaggaaa ccggcaccta ttacgtcaac gcccgctcgt tcgataacgt gcctgaggac
     7321 ggcaccaacg gcgacgtcgt cggcgactat gagttgttcg tcgacgaagt cgatccgaac
     7381 gatcccaacg tctaccggcc ttattacgag acggacagcc cgctctactc gcttgattgg
     7441 ggaacgcagg tcaaccgcgt ccaccagacc gtgcgcaatc ccgatggcga ggaagggccc
     7501 cggcccaccg gcaatgagca ggaagcgccg ccggagaatg cctacaacat caccgacaag
     7561 aacgtcatca ccatctactt cgccagagaa ggcgacgtct tcgcctcgga agatccgacc
     7621 aaccccggcc tgccgccggt gctcgtttcg gccggaacca aggattggga gaaggaagtc
     7681 gtcaacacgg cgctcgatca gatcgaagaa gtggccgacg tcgtctacgt ggaggtcgac
     7741 aatcgggaag aagccgactt cttcttcacc acttatgcgg gcacgcccgg acccggcgtc
     7801 agcctgctcg gctcgatggc cccgccggat tatcccgatg aaggtttggc tcagttcaac
     7861 tcgggcgatt atcgctggac cgagaagaac ctgcagcagg gcggattttc ctacgtcacc
     7921 ctggttcacg aattcggcca cggcatgggc atggcccacc cgcacgacaa tggcggccgc
     7981 tcgggcatca tgaacggcgt tgaggaagaa agcaccttca attactcgac cggcgatttc
     8041 aacctcaacc aaggcatcaa cacgatgatg tcctacgagg acggttggca gatgtcgccc
     8101 tacggcaatg cccccaccga cgccggctac ggttatctcg gcggcccgat ggccttcgat
     8161 atcgccgttc tccaggacaa gtatggcgtt aacgaggacc atgcgaccgg caacaacatc
     8221 taccggatga aggacgtcaa cgccgcgggc accttctacg actcgatctg ggatgccggc
     8281 ggtcacgatc agatcatcta tgatggcgag cgcaactcga aggtcgatct tcgcgacgcg
     8341 acgctcgaat atgagattgg cggcggcggc ttcatctcct acgcgcatgg catctttggc
     8401 ggctatacga tcgccaattc cgtgacgatc gaggacgccc gcaccggttc cggcaacgac
     8461 ctgatcaacg gcaacgaggt cggcaaccgc ctgatctcca acgccggcca cgatacggtc
     8521 cgcggccttg gcggtcagga tgccatcttc ggcggtgtcg gcaacgacaa gctgttcggc
     8581 ggcagccaga acgacaacat gtcgggcggc gaggggctgg acttcgtgct cggccagacc
     8641 gggaacgacc gggtgtcggc cgatgcgggc aatgacgtcg cccgcggcgg cgacggcaac
     8701 gaccgcgttg ccggcggcga gggcgacgat cgcatcaacg gcaaccttgg caacgacgtt
     8761 gtcatcggcg gcgcgggaat cgacatgatc gctggcggcg gcgatgccga catcttcgtc
     8821 ttcggagaag gcgatagcgg cagcagccgg agcacggcgg atcacatcct cgacttcagc
     8881 agcgacaatg gcgacagcat caatctggcc cagatcgacg ccatcgccgg aaccgcggcc
     8941 aacgactcct tcaccttcat cggcaaggga gccttttcgg ggaccgcggg cgagctgcgc
     9001 tacttcgtca gcgacggcga cgcgatcgtc tcggcggaca cggacgggga caagctcgcc
     9061 gacttcgtgc tcgtcatgga cggccggagc cagttggccg cgaccgactt cctggtctaa
     9121 gcctgacccg atcggcggcg gcgcccggcg ccgccgccaa cagccccggc ttcggccggg
     9181 gctttttttt gggccactgc caaccgggcg agcggctagg ccggggtgta gggtgccgcg
     9241 cggatccagc ggctgcagat cagcttggtc ccctgcgtga cgggcaggcc ggcatggccc
     9301 gcatccttgt cggggcggcc ctcatcatcg acgttgcgga agaccagggc gtcaccgcgg
     9361 cctcccttga ccttgagctg cggcgtctcg aaccaagtct cgccgccttc atagccctcg
     9421 ttcagataga ccagaacggt cagaattctc tggttcgcga agccgggaat ggcgtcgaaa
     9481 tgggggcgat attgctgccc gccccggtag cgcagcacct gcagcggctc gccttgggcg
     9541 acatcggtct gcgtgagccg ggcgatgcgc cggttgagcg cgtgaacggc cgggttctcg
     9601 agcggccagg taaagcccat tccgtccgac gtgcggccag ggttcggaat ctggcgcccg
     9661 gtggcagggt ccaccaccac cgacggcgcc agcatcggct ccgcggccgc ggtgacatag
     9721 tcgcactcga ggggagagaa gaattcccga gcgagttcca cccgtgggca ctcgctcagc
     9781 cgctcgcgcc gccaagtccc ggcgggctcg ccctcggggc cgagctccat cgccccgatc
     9841 acctcgagct cgcgggccga ccggggattg acccgggcca actcgcgcag cagcgccaat
     9901 ccgcgcggcc aatcggccgc cgcgccgacg ccgctcgcga cgaaattcgc gtagatcacc
     9961 gcggcgtcct tgcggccggc tacggcggcg cggcggagca tctcgcgggc gaccgccagg
    10021 tcgcgcggaa ccgcctcgcc ggcaaggctc agcgccccaa gctcgaacaa ggccgaagca
    10081 tcgcccgccg cggcacggcg tttcagctcc ccgatatgat ccgctgccat tgccgtgtaa
    10141 tgcgcggagc ggcgcaaaag gccaagctgg gcttggcagc ccgcgctttt tttgcttttg
    10201 gaacaagact attgccgcta gggtccgcgc ggggttaggc gccgcgggga ttctcctgca
    10261 aggtcaacgg tgtgcgctct gatatgggag ccggcgcgct gacgccagcc ttgatttttg
    10321 cttaggatcg gctttgttga gcatgctcgt ttcgcttttc gctctctcca cggcgctcgc
    10381 ccaggcgcag gcgccgtccg gctccccgct ggcgccgccg ccgacggcca ttccggtctc
    10441 gccccaggtc caggacctgc cgcagccgac ccgggacccg ctccgcatcg accgcaacgc
    10501 cgatccggtg ctgggcctcg cccgcaatag cgcccgtccg accgagttcg tcgggctgat
    10561 tcagcaggcg gtcgagcgcc atccggcggt cgacgaagcc gccgccggca tcgaggaagc
    10621 ggaggccgca accgaagagg cgcgggccgg ccgctatcca accgtcgacc tctcgctcaa
    10681 ttcgcagcgc tcgatcatcc gcaatttcga ggataacgac cccaacaata tcgtcgagcg
    10741 cgcccgcgcc cgcggccgca gcgacctcac cctgacggtg cagcagaccc tgttcgacgc
    10801 cggcgcgacc gagaagcgca tcgccgcggc cggcgcccgg ctccgcgccg ccagcttcca
    10861 gaccgaggcg caggccgacc agatcgcgct ccgcgccatc gccgcctggt atgatgtgtt
    10921 cgcctaccgc gccctggtgg agctcggcgg ggcgttcatg gagaaccagc aggagctgag
    10981 gagcgccatc gagatccgca tcactcaggg cgtctccgcc cccggcgacc tgccgcgggt
    11041 cgagagctac atcgcctccg ccgatgccga cctcgctcgc taccggcggc agctcgccaa
    11101 tgccgaggcg cgcttcgaag agatgttcgg agtgccggcc ccgccggggc tcggccgggc
    11161 gccgatcatc tcccgccctt cgaccaccaa ggacgccgcc caattcctcg cccgctcggc
    11221 cccttcggtc gaggcggccg aggccaatgc ccgcgccagc cgccaggagg cgcgtgccga
    11281 gcgcgcctcg acgctgccga cgatcaccgc cggcattgac gccggcaaat acggacttgt
    11341 tgaccaaaac gactatgacg tgcgcggccg cgtgacgctg cgccaccgtt tcttcggcgg
    11401 ggttgatgcc cgcgccgacc aggccgaggc gcgggccaat gccgccgaag cccgcgcgtc
    11461 gcgcgtccgc aacgaagccg agcgcgaggc ggcgatcgcc tggtcggacg ttcgcgcgct
    11521 ggagcagcag ctggcggcgc tggagaatag ctatatcgcc agccgccagt cgcgcgacgt
    11581 gctggccgag cgcttccgcg ttgcccgcgg gacgctgttc gacctgctcg aatccgaaag
    11641 cggctatttc aatgtcgccg cgacctatat tcgaacgatt acggagctcg acgcggcgcg
    11701 ctatgtgctg ctgtcgcgga cggggcaatt gctgccggtc ctccagatcg agcccgcccg
    11761 gttggaggtg caatgagcga cgatacaata gtcgagatcc cgaagcagcg gttcgcgccg
    11821 tggctgatgg agccgatgaa gcgcaacaag ggcatctaca tcaaggtcgg cctcgccgcc
    11881 gccttgatca acatgttcgc cttactgacg tcgatcttca cgatggtcgt ctacgaccgg
    11941 gtgctgccga acaacgccac ctcctcgctg atcgcgctgt cgatcggcct cgcgatcgtc
    12001 atcgtcttcg acttcgcatt gaagaccctg cgcgcctatt tcgttgacgt cgccggcgcc
    12061 cgcatcgacc gcgaggtcgg ggagtcggtg ttcgcccaat tgctcgccat ccgcctcgac
    12121 cagaagaagg gatcgaccgg cgccctcgcc ggcgtgatcc gcgagctcga gacgctgcgc
    12181 gacttcttcg cttcggcgac tttgaccgcc gtggtcgacg tgccgttcat cttcgtcacc
    12241 ctgggggtga tcgcgctgat cggcgggtgg gtcgtcaccg tgccgctgat catgatcccg
    12301 ctcgtggtgc tggtcggatt gatcacccag cccgccatgg accggctcgc cgcccagtcg
    12361 atgggccagg gactgctcaa gcagaccgtg ctggtcgaga ccatcggcgg cctcgagacc
    12421 gtcaagacgt cgggcgccgc gccgatcctc accgaacgct ggctcggcgc ggtcgaggag
    12481 catagcagca ccagcatgcg ccagcgcctg gtcgccaata tcggcgtcac catcgccggc
    12541 tcggcccaga cgatcagcta tgccggcgtg gtcatcgtcg gcgtcggcat gatcgccgag
    12601 aatacgctga ccatgggcgg cctcatcgcc tgctcgatcc tttcgggccg cgcggttgcg
    12661 ccgctcggcc agatcgcgac cctgctgtcg cgcatgaccg cgacgcgcac ggcctaccgt
    12721 cagctcaacg gactgatgag cacccggccg gagggccccg agggcgagcc gctggtgctg
    12781 acccagacgt cggggcggat cgaattccgc aacgtccatt ttcgctatcc cggcgcgccc
    12841 gaaaaggcgc tcgagggcgt caccttcacg ctccagccgg gcgagcatgt cgcgctgctc
    12901 ggccgcgtcg gctccggcaa gtcgacgatc gcgcggctgc tgctcggcct ctatgagccc
    12961 gaggagggcg tgatcctcct cgacggcacc gacatccgcc agctcgatcc gctcgacctg
    13021 cgccggaagg tcggcagcgc gatgcaggag agcatcctcc tgtccgggtc ggtgcgcgag
    13081 aatatcgtgc tcggccggca cggcatcggc gacgacgaga tgatccaggc ggccgagctt
    13141 tccggcaccc accagttcat gggccagatc gccaatggct acgaccgcag gctcgccgac
    13201 cgcggcgagg gcctgtcggg cggccagcgc cagtcgatcg ccatcgcccg cgcgctcgcc
    13261 accaagccga atgtgctggt gttcgacgag ccgaccagcg cgatggacca gcagaccgaa
    13321 agcgggctgc tcgaccggct cgaccgcgag ctcaaggatc gcaccttcct cgtcatcacc
    13381 caccgcacgc cgttgctgcg gctggtcagc cgggtcatca tcctcgaccg cggcaagatc
    13441 atcgccgacg ggccgcgcga tcagatcctg gccaagctca accgtccgaa ggtcgccgcc
    13501 gtatgaaccg cgaagtcgct ctcgaagact atatcaagcg cgtccgtccc gcgaaggcat
    13561 cgaacatcct gttatgggtg atcgccggtt tcgtcgtggt cttcgtcgtc tgggcggcgc
    13621 tgaccgagct cgaccgaacc gtgcgcggcc agggccggat catcccatcc tcgcagctcc
    13681 aggtcgtgtc caacctcgag ggcggcgtgg tcgaggcgat tctggtcaag accggcgaca
    13741 tggtgcagcg cggcgctgcg ctggtgcgcc tcgaccagac ctcgaccggc gccgagctcg
    13801 gcagcaacca ggcccagttc gacgccctgc gcgtccgcat cgcgcggctc gaggccgagg
    13861 tcgccggccg ctctcccaat tttccgcccg ccgtcaattc gagcctcgcc gagcaggtcg
    13921 cgatcgagcg ctcgctccac gcctcgcgca tggccgatct tggcagcctc ctcgccgccg
    13981 ccaatgcccg ggtggtccag gcgcagcgca gcgtcaccga ggcgcaggcg tcgctcgccg
    14041 ccagacagtc cgcggccggg gccgcccgca ccgaagccaa ccttattcgc ccgctggtcg
    14101 agcgcggcat cgagccgcgc ctgtcgctga ttcaggccga gaaccaggcc gccgtttccg
    14161 ccgccgaggc cgctgccgcc tcggccgcgg tgaaccgcgc gcaatcttcg gtggccgaag
    14221 cccaggccac actcgcccag cagcgccagg attggcgcgc ccgcgcctcg caggagctcg
    14281 cttcggccca ggccgagatg gtcgcgctgc gccgctcgct gcccgcattg tcggcgcggg
    14341 tcgagcggac cgtggtgcgt tcgccgctcg ccggccgcgt caatcgggtg ctcgtcacca
    14401 ccgtcggcgg caccatcgcc cccggcggcc cgatcgccga gatcgtcccg agcgaggatg
    14461 cgctcctcgt cgaggtccag gtccgccccg ccgacatcgc ctgggtgcgg atggaccaga
    14521 atgcgaagat caatatcacc gcctacgatt cgtccgtcta cggggcgctc gacggcaagg
    14581 tggtggcgat ctcgcccgat gcgaccttga acgagcgcac cggcgagacc ttctacacag
    14641 ttcgggtggc gaccaccgac gagctgttcg acaagagcgg caaagagctc gagatcgggt
    14701 cgggcatggt cgccgacatc agcctgctcg gcgacaagcg caccgtgctg agctacatcc
    14761 tgagcccctt cacccgcctc agcgagactg ccctccgcga ggagtgatcc ggccggccgt
    14821 cgcggcggcc cgggcctgcc gcgtcggcgg tcggtttggg ctaccgccgc agccagcgaa
    14881 ctccgcgagg ggcggtccgg ccggcccgct gcggtgtaca aggccagctc cgatgtcgac
    14941 ccgggcggcc ggtgccatga acctgcgcgc gcttggatgc cgctgctgtc gtgccgctat
    15001 gcggatc
//
LOCUS       DASVRK010000147         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_90144, whole genome shotgun sequence.
ACCESSION   DASVRK010000147 DASVRK010000000
VERSION     DASVRK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90144"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="VF589_05905"
     CDS             <1..1564
                     /locus_tag="VF589_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8257146.1"
                     /translation="VAQTQAVLARELSGGSDEAARLFRQSVNLTRDIERARVEISRLS
                     AAAQPSAADAVRLAELRPQLATMERDQAATQSKLAAFPRYRVLSGGAMSLTELQKVLR
                     EGEAYYKMTMAGDEGYAIFATRHSARGYRIGARAAELEQQVDALRATIAKVENGQLVT
                     YPFDVQLAHKLYGQLFQPVEAELAAVRHLIFEPDGAMLRLPPNLLVTDHAGVQAYLAK
                     ANRPTDDGFDFTDIAWLGAGRDVSTAVSARSFRDVRNVAPSAARNDYIGFGENAPPAG
                     VVRTAGGVRGLSDAGAGCSWSLNAWGSPISSAELVTASRTLGGGADTQIVTGQAFTDT
                     AIKERDDLSQYRIMHFATHGLVTAPRPECPARPALMTSFGEQGSDGLLTFAEIFDLKL
                     DADLVILSACDTAGKASVAATREAGLTTGGDFALDGLVRAFVGAGGRSVIASHWPVPD
                     DYDATNRLISGLFTARPGTSTATAMRNAQSALMRQRETSHPYYWSGFAIIGDGAAPVI
                     PAAEGSRQALSR"
     gene            1662..>2620
                     /locus_tag="VF589_05910"
     CDS             1662..>2620
                     /locus_tag="VF589_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257147.1"
                     /translation="MIAALLAGSSLASAAVAQTESQPSDAGEAAANPGAPIVSDRDFE
                     AQLPPIDPALNGPLEPIESFDMPAAPAAEAGQSAPGETIADAPLPDAALTEPLPPIAT
                     FDVTTPPPTAGDAEPPPVRYTLAIEGLEAVGLEGRFRELSALEDADGEAANGAMIAAR
                     AREDETLAVRLLRSEGYYDAAALATTEQIPEQPGQLRVTLTAVPGQRYAFGAIDLPGA
                     ATVPPGLAREALTLEPGKPIVAAEVEGAEANVLLRLPEQGYPFASVGQRDILLDPATA
                     LGDYTLPVEPGIRARFGGYTTEGDLAFDAEHVGVLARFDRGEL"
BASE COUNT          379 a         1018 c          837 g          386 t
ORIGIN      
        1 cgtcgcccag acccaggcgg tgctcgcccg cgagctttcg ggcggcagcg acgaggccgc
       61 ccggctgttc cgccagtcgg tgaacctcac ccgcgacatc gaacgcgccc gggtcgaaat
      121 cagccgcctt tcggctgccg cccagcccag cgccgccgac gccgtccgcc tcgccgaact
      181 tcgtccgcag ctcgcgacga tggagcgcga ccaggcggcg acccagtcca agctcgccgc
      241 ctttccccgc taccgcgtgc tttccggcgg agcgatgagc ctgaccgagc tgcagaaggt
      301 gctgcgtgag ggcgaggcct attacaagat gaccatggcc ggcgacgaag gctatgcgat
      361 cttcgccacc cgccattccg cccgcggcta ccggatcggc gcccgcgccg cggagctcga
      421 gcagcaggtc gacgccctcc gcgccaccat cgccaaggtc gagaacggcc agctcgtcac
      481 ctacccgttc gacgtccagc tcgcccacaa gctttacggc cagctgttcc agccggtcga
      541 ggccgagctc gcggccgtcc ggcacctcat cttcgagccc gatggcgcga tgctcaggct
      601 cccgcccaac ctgctcgtca ccgaccatgc cggcgtccag gcctatctcg ccaaggccaa
      661 tcggccgacc gacgacggct tcgacttcac cgatatcgca tggctgggcg ccggccgcga
      721 cgtcagcacc gcggtctcgg cccgttcgtt ccgcgacgtc cgcaatgtcg ccccgtcggc
      781 cgcgaggaac gactatatcg gcttcggcga gaatgctccg cccgccgggg tggtgcgcac
      841 cgccggcggc gtgcgcgggc tctccgacgc gggcgccggc tgctcctggt cgctcaacgc
      901 ctggggaagc ccgatctcgt cggccgagct ggtcaccgcc agccggactc tcggcggcgg
      961 cgccgacacc cagatcgtca ccggccaggc cttcaccgac accgccatca aggaacgcga
     1021 cgacctttcg caatatcgga tcatgcattt cgccacccac ggcctcgtca ccgcgccgcg
     1081 gcccgaatgc cccgctcgcc cggcgctgat gacctcgttc ggcgagcagg gatcggacgg
     1141 ccttttgacc ttcgccgaga ttttcgacct caagctcgac gccgacctcg tcatcctctc
     1201 ggcctgcgac accgccggca aggcgagcgt cgccgcgact cgcgaggccg gcctcaccac
     1261 cggcggcgat ttcgcgctcg acgggctggt gcgtgccttc gtcggcgccg gcgggcgctc
     1321 ggtgatcgcc agccattggc cggtcccgga cgattatgac gcgaccaacc ggctgatttc
     1381 cggcctcttc accgcgcggc ctggcacgtc gaccgcaacc gccatgcgca atgcccagtc
     1441 ggcgttgatg cggcagcgcg aaacctcgca tccttattac tggtcgggct tcgccattat
     1501 cggcgacggc gccgcgccgg tgatcccggc cgccgaaggc tcgcgtcagg ctctatcccg
     1561 ctgaaaaaat atgagataaa ggcgtcgccc gggcgcggca cttttttggc caaagatcgt
     1621 ttcgcctcgg taatgggggc gggttgttga acaaagctgg cttgatcgct gcacttctag
     1681 ccgggagttc gctcgcttcc gccgccgtgg cgcagaccga aagccagccc agcgacgccg
     1741 gcgaggccgc tgccaatcca ggcgcgccga tcgtctcgga ccgcgatttc gaagcccagc
     1801 tgccgccgat cgatccggcg ctgaacgggc cgctcgagcc gatcgaaagc ttcgacatgc
     1861 ccgccgctcc ggcggccgag gccggccaga gcgcgcccgg cgagaccatc gcggacgccc
     1921 cgctccccga tgccgcgctg accgagccgc tgccgccgat cgccaccttc gacgtcacca
     1981 cgcctccccc caccgccggc gatgccgagc cgccgccggt gcgctacacc ctcgccatcg
     2041 aggggctgga ggcggtcggc ctcgaaggcc gcttccgcga actgtccgcg ctcgaggatg
     2101 ccgacggcga ggcggccaac ggcgcgatga tcgccgcccg ggcgcgtgag gacgagacgc
     2161 tcgccgtgcg gctgctccgc tccgaaggtt attatgacgc cgccgccctc gccacgaccg
     2221 agcagatccc ggagcagccg gggcagctgc gcgtcaccct caccgccgtt cccggccagc
     2281 gctacgcttt cggcgcgatc gacctgcccg gcgcggcgac ggtgccgccg ggcctcgccc
     2341 gggaggcgct gacgctcgag ccgggcaagc cgatcgtcgc cgccgaggtc gaaggcgccg
     2401 aggcgaacgt gctgctgcgc ctgcccgagc agggctatcc gttcgcctcg gtcggccagc
     2461 gcgacatatt gctcgatccc gccaccgcgc ttggcgacta cacgctgccc gtcgagcccg
     2521 gcattcgcgc gcgcttcggc ggctacacca ccgagggcga cctcgccttc gacgccgaac
     2581 atgtcggcgt gctcgcccgc ttcgaccgcg gcgagctctt
//
LOCUS       DASVRK010000148         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_90429, whole genome shotgun sequence.
ACCESSION   DASVRK010000148 DASVRK010000000
VERSION     DASVRK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90429"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1662
                     /locus_tag="VF589_05915"
     CDS             <1..>1662
                     /locus_tag="VF589_05915"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8257148.1"
                     /translation="FDVVAGQTYMVSMRGTGTDPLVDSYLTLRNQNQGVVTIDDDGGV
                     RTNSLITFTANRTGQWRVSAEGFPGDGGTGEYRIDFRKAGTDAVPGGTHSNVALAPNA
                     TTLGFIETSGDIDVYKIELEAGLFYTFELAGGADQETDAAAMPAGELDTIIGLLDAQG
                     REGASNDDLSFPYDISSGVGFGPTKSGTYYVQVEAYPGETGGYALEVQEIDVAGLDPL
                     DSIDWGTQVASNHVTVYFAAAGERFGGMTSNGWTNYQKGRAMAALEEYAEVSGLTFSV
                     ATSRNGATFKLVTTESAEYLGIFTPPGETGAGVGAFAVNGEGWDNVGGLAQGGFGFVT
                     LLHEFGHGLGLAHPHDDGGTSTIMPGVAGPFGSLGPYDLNQGVYTTVSYNDGWAKHPD
                     AVDGAPPGFPTTYGWQGGPSALDIAVIQQKYGANTGHNSGNDVYTLPTANRGGTFFTT
                     IWDGGGDDKMQYSGLAAATIDLTAATLDFSPTGGGVVSYADGIFGGFTIAHGVVIENA
                     RGGRGADTLVGNAAANILDGGAGADTMTGGAGDDRFVVDNIGDIV"
BASE COUNT          286 a          588 c          543 g          245 t
ORIGIN      
        1 ccttcgacgt cgtcgccggg cagacctaca tggtctcgat gcgcggcacc ggcaccgacc
       61 cgctcgtcga cagctatttg acgctgcgca accagaacca gggcgtcgtg acgatcgacg
      121 acgacggcgg ggtgcggacc aactcgctga tcaccttcac cgcgaaccgc accggccagt
      181 ggcgggtcag cgccgagggc ttccccggcg acggcggcac cggcgaatat cggatcgact
      241 tccgcaaggc cggcaccgac gccgtcccgg gcgggaccca cagcaatgtc gcgctggcgc
      301 ccaatgcgac gacgctcggc ttcatcgaga cttcgggcga catcgacgtc tacaagatcg
      361 agctcgaagc gggccttttc tacacgttcg agctggccgg cggcgccgac caggagaccg
      421 acgccgcggc catgccggcg ggcgagctcg acaccatcat cggccttctc gacgcccagg
      481 ggcgcgaggg cgcgtccaac gacgacctca gctttcctta cgatatcagc tccggcgtcg
      541 gcttcggccc gaccaagagc ggcacttatt atgtgcaggt cgaggcctat cccggcgaga
      601 ccggcggcta cgcgctggag gtccaggaga tcgatgtcgc cgggctcgac ccgctcgatt
      661 cgatcgattg ggggacccag gtcgcgtcca accacgtcac cgtctatttc gcggccgccg
      721 gcgagcggtt cggcggcatg acctcgaacg gctggaccaa ttaccagaag ggccgcgcga
      781 tggcggcgct cgaggaatat gccgaagtct ccggcctcac cttctcggtc gcgaccagcc
      841 gcaacggcgc gaccttcaag ctcgtcacga ccgaatccgc cgaatatctc ggcatcttca
      901 cgccgccggg cgagaccggg gcgggggtcg gcgcctttgc cgtcaacggc gagggctggg
      961 acaatgtcgg cggcctcgcc cagggcggct tcggcttcgt caccctgctc cacgaattcg
     1021 gccacggcct cgggctcgct cacccgcatg acgacggcgg cacgtcgacg atcatgccgg
     1081 gcgtcgccgg cccgttcggc agcctcgggc catacgacct caaccagggc gtctatacga
     1141 cggtgtccta caatgacggc tgggccaagc atcccgacgc ggtcgacggc gccccgcccg
     1201 gcttccccac gacctatggc tggcagggcg gcccctccgc gctcgacatc gcggtgatcc
     1261 agcagaaata cggggcgaat accgggcata acagcggcaa cgacgtctac accttgccga
     1321 ccgcgaaccg gggcgggacc ttcttcacga cgatttggga cggcggcggc gacgacaaga
     1381 tgcaatatag cggcctggcc gccgcgacga tcgacctcac cgccgccacg ctggatttct
     1441 cgcccaccgg aggcggcgtc gtgtcctatg ccgacggcat attcggcggt ttcaccatcg
     1501 cccacggggt ggtcatcgaa aatgccaggg gcggccgcgg cgccgacacg ctggtcggca
     1561 acgcggccgc caacatactc gacggcggcg cgggcgccga tacgatgacc ggcggcgcgg
     1621 gcgacgaccg cttcgtggtc gacaatatcg gcgatattgt ca
//
LOCUS       DASVRK010000149         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_401942, whole genome shotgun sequence.
ACCESSION   DASVRK010000149 DASVRK010000000
VERSION     DASVRK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401942"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VF589_05920"
     CDS             <1..514
                     /locus_tag="VF589_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257149.1"
                     /translation="YMLALTLILAALAPTAQAQAALPKWVPGVHVSEDSVRIVHDIPV
                     VTNQRTRQVILDLLHDFHRLGDSQFKKKYSNLKLVDVVAKKVRDTTDVKLREAARSDV
                     ASDLKGCAGLFLGDMGKSNDSAEQFLFLNFDCGSHQRYFGLTLTSERPTSLYMHHDYL
                     VSVHTGPPRL"
     gene            685..864
                     /locus_tag="VF589_05925"
     CDS             685..864
                     /locus_tag="VF589_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257150.1"
                     /translation="MFTDEIIAVIAGAIKSEIAHPLGAMRHLPVPEEEVALTILNALD
                     EAGFQVIVREQQPSG"
     gene            complement(966..>2853)
                     /locus_tag="VF589_05930"
     CDS             complement(966..>2853)
                     /locus_tag="VF589_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8257151.1"
                     /translation="MDETQARAILKTFDTASGRADTVAAVPLYPFAGELSVRVAPDRD
                     RLAIMATTAMIPPAAGRPITRLDDGWLVERRLGFVDLGGNKALRWAAMPAEARYPLDL
                     LDWSPDGGRVALRARRGPADPSAPLYAASADGLAVERVGPEIASVGDASTNTSFPRDS
                     ASIWLDGRRLLVRATRGADGRAGWWLAEAGGRATPLPGGEAHPLSAFHRLATGELVGI
                     AGERLVRLDPSTGTLVPVAGAPLPAGARILQQEGALGPAGPIVVAERGAAGVQTLRLL
                     PLAPGAAATRPLTLPAGAEILHVDAARATVIWREPTRRGLFLREASLAGGRKRDLLAL
                     NGHLANVSWGETRLIDYRIADGRALKGAVILPPGYRAGRRYPVVTWVYGGHQVSGLDD
                     YWLDPSMPGLYNLQLYAARGYVVLVPSIPLERSGRNTHLTELTKSVLPAVDRLVELGI
                     ADGARVGVMGQSYGGFAVMGLATQTDRFKAGVALAGMSDLTAFHYAFDPTARFYPGIE
                     HEKSSNPAIAEAGNAGLAVPPYEDRWLYWRGSPLSHADRIETPLLIAHGEFDVRGSLA
                     QSEALFAALHRQGKTARLLRYWGENHALATSPANVRSVLAEALAWFDKYLAKPAAAPP
                     RPAD"
BASE COUNT          490 a          910 c         1009 g          444 t
ORIGIN      
        1 gtatatgttg gctttgaccc ttattctagc cgcgctggca cccactgcac aggcgcaagc
       61 agctctcccc aagtgggttc cgggcgtgca cgtcagcgaa gacagtgtgc gtatagtcca
      121 tgatataccg gtcgtgacca atcagcgaac gcggcaggtg atactggact tacttcatga
      181 tttccatcga ctcggcgatt cccaattcaa gaaaaaatac tccaacctta aactggtaga
      241 cgtagtcgcc aaaaaagttc gcgatacgac agacgtgaag ctacgtgagg cggcgagatc
      301 cgacgttgca tcggatttga agggatgcgc aggtctgttt cttggcgata tgggcaaatc
      361 gaacgacagc gcagagcagt ttttgttctt gaacttcgat tgcggatcac atcagcgata
      421 tttcggcctt acattgacga gcgagcggcc aacctcgctc tatatgcatc acgattatct
      481 tgtatcagtc catactggtc ctccacggct atagtgaagc gtcgggaacg tcgcccaagt
      541 cggcaagctc cccgatcgcg ccaagcgaag cgctgcgcca cggattcgcc ggcggggcgg
      601 cgattagagg ctagggacgc gccggcggcc acccagcccg gccggacgtt gcgggtggat
      661 ctggcagcgc ttagtctgac actcatgttc accgacgaaa tcatcgctgt gatcgcaggg
      721 gcgatcaaat cggaaatcgc tcatccgcta ggcgcgatgc ggcacctgcc cgtgcccgag
      781 gaggaggtcg ccctcacgat cctcaacgcg ctcgatgaag ccggcttcca ggtgatcgtc
      841 cgagagcaac agccgtccgg ctagggtccg caatcgctcc gaagcaagct ttcgggaggt
      901 gcatcgagca gatatcgacc ctggtgagat cgagacaggt gccgaacatg cactcaaggc
      961 attcctcaat cggccggccg gggcggagca gcagcgggtt ttgcgagata tttgtcgaac
     1021 caggccagcg cctcggcgag gacgctgcgg acgttggcgg gggatgtggc gagcgcgtgg
     1081 ttctcgcccc aatagcgtag gagccgcgcg gtcttgccct ggcggtggag cgccgcgaac
     1141 agggcctcgg actgggcgag gcttccgcgc acgtcgaact cgccgtgggc gatgaggagc
     1201 ggcgtctcga tccggtcggc atggctgagc ggcgagccgc gccaatagag ccagcgatcc
     1261 tcataaggcg gcacggcgag gccggcgttc ccggcttcgg ctatggcggg gttgctcgat
     1321 ttttcgtgct cgatgccggg atagaagcgc gcggtcggat cgaacgcata atggaaggcg
     1381 gtcaaatcgc tcatgccggc cagcgcgacg ccggccttga accggtcggt ctgggtcgcc
     1441 agccccatca ccgcgaagcc gccatagctc tgccccatga cgccgacccg cgccccgtcg
     1501 gcgatgccga gctcgaccag ccggtcgacg gccggaagca cgctcttcgt gagttcggtg
     1561 agatgcgtgt tcctgccgct gcgctccagc gggatcgaag ggacgagcac gacatagccg
     1621 cgggccgcat agagctgcag attgtagagg cccggcatgg agggatcgag ccaataatcg
     1681 tcgagcccgc tgacctggtg gccgccatag acccaggtca cgaccggata gcgccgcccg
     1741 gcccggtatc cgggcggcag gatgaccgcg cccttgagcg cgcggccgtc ggcgatgcga
     1801 tagtcgatca gccgggtttc gccccagctg acattggcga ggtggccgtt gagggcgagc
     1861 aggtcgcgct tgcgcccgcc ggccaaagat gcctcgcgca agaacagccc gcgccgggtc
     1921 ggctcgcgcc agatgacggt ggcgcgagcg gcatcgacgt ggaggatttc ggcgccggcc
     1981 gggagggtga gcggccgggt agcggcggcg ccgggtgcga gcggcagcaa gcgcagcgtc
     2041 tggacgccgg cggctccgcg ctcggcgacc acgatcgggc cggcgggccc gagcgcgcct
     2101 tcctgctgta ggatgcgggc gcctgccggt agcggcgcgc cggccaccgg aacgagcgtg
     2161 ccggtcgagg ggtcgagccg caccagccgc tcccccgcta tgccgaccag ttcgccagtg
     2221 gcgagcctgt ggaaggccga gagcggatga gcttcgccgc cgggcagcgg ggttgcgcgg
     2281 ccgccggcct cggcgagcca ccagccggcg cggccgtcgg cgccgcgcgt ggcccggacc
     2341 aggagccgcc ggccgtcgag ccagatcgag gctgagtcgc gcggaaagct cgtgttggtc
     2401 gaggcgtcgc cgaccgaggc gatttcgggc ccgacccgct cgaccgcgag gccgtcggcg
     2461 gaggcggcgt aaagcggcgc cgacggatcg gccgggccgc ggcgggcgcg cagcgcaacc
     2521 cggccgccgt cgggcgacca atcgagcaga tcgagcgggt agcgcgcctc cgccggcatg
     2581 gccgcccagc ggagggcttt attgccgccg aggtccacga agccgagccg gcgctcgacg
     2641 agccagccat cgtcgagccg ggtgatcggc ctgccggccg ccggcgggat catcgccgtc
     2701 gtcgccatga tggcgagccg atcgcggtcg ggcgcgaccc ggactgacaa ttcgccggcg
     2761 aacgggtaga gcggcaccgc cgccacggtg tcggcgcggc cgctcgcggt gtcgaaggtt
     2821 ttcaggatcg cgcgcgcctg cgtctcgtcc atc
//
LOCUS       DASVRK010000150         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_28468, whole genome shotgun sequence.
ACCESSION   DASVRK010000150 DASVRK010000000
VERSION     DASVRK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28468"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..>1710)
                     /locus_tag="VF589_05935"
     CDS             complement(33..>1710)
                     /locus_tag="VF589_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257152.1"
                     /translation="PVGGICVSGRAYDDLRRLGLPFESIGKQQLKNIDEPIEVYQLSA
                     EQLAALPMEEAPADTPGSRRSLLRPVPIALAVAALALLALVLWRPWSAAASPPSVTVV
                     AGGGGAEAQALARNLLVQLGSLQATKADALELVEPESGTRADLIFKVESVGGEGDLRA
                     NLSLVDNRGDTLLWSREVVQPGGNRTDLRQQIAYSAARVLGCATEALSPLHEPIALPT
                     LRLYLNGCADLADLLVQQDSAIAVATFRKVTEQAPAFAGGWAKLLLAQIQAFRGAGLN
                     DPALRREIEASIAAARKLDPDMAEALLAEGWLQPRRPIAGWLRFSDEAVAKNPDHAEA
                     LGMRSLGLVQVGRMREAVDDARRAVGVEPLSPFARKALIHRLMDSGATGAARNALREA
                     ERLWPGATDLVYSRFVLEYGYGDAGEAWRILEAGGLNFPPTAAQKSFIEARIDASPAK
                     VERALADAREAFERDRGTLDDYAQALAQFGRGEEAIRALLSADPRIAPDVVGALFRPP
                     FEALHADPRFMAVAQRFGLVQYWRDSGRWPDFCSRPGLPYDCKAEAAKLR"
BASE COUNT          280 a          611 c          587 g          232 t
ORIGIN      
        1 cggggtcgcg atgatgcggg aggcgggcgt gcctagcgca gcttcgccgc ctcggccttg
       61 caatcgtagg gcaggcccgg gcgggagcag aaatccggcc agcgcccgct gtcgcgccaa
      121 tattggacca ggccgaaacg ctgggcgacc gccatgaagc gcgggtccgc gtgcaaggct
      181 tcgaagggcg gccggaacaa ggctcccacg acgtccggcg cgatgcgggg atcggcggaa
      241 agcagcgcgc ggatcgcctc ctcgccgcgc ccgaactggg ccagcgcctg tgcataatcg
      301 tccaaagtgc cgcgatcgcg ttcgaaggct tcgcgggcat cggccagcgc ccgctccacc
      361 tttgccggcg atgcgtcgat gcgcgcctcg ataaagcttt tctgggccgc ggtcggcggg
      421 aagttcaggc cgcccgcctc gaggatccgc cacgcctccc cggcatcgcc gtagccatac
      481 tccaggacga agcgggaata gacgagatcg gtggcgccag gccacagccg ctccgcctcg
      541 cgaagcgcgt tccgcgccgc gccggtcgcg ccggaatcca tcaggcgatg gatcagcgcc
      601 ttgcgcgcga acggagacag cggctcgacg cccaccgccc gccgggcatc gtcgaccgcc
      661 tcgcgcatgc ggccgacctg gaccaatccc agcgagcgca tgccaagcgc ctccgcgtga
      721 tcggggttct tcgcgacggc ttcatcgctg aaccgcaacc agccggcgat cggccggcga
      781 ggctggagcc agccttcggc gagcaaggct tccgccatgt ccggatcgag cttgcgggcc
      841 gcggcgatac tggcctcgat ctcccggcgc agcgccggat cgttgaggcc cgcgccgcga
      901 aaggcctgga tctgggcgag cagcagcttg gcccagccgc cggcgaaggc cggggcctgc
      961 tcggtgacct tcctgaacgt cgcgaccgcg attgcggaat cctgctgcac gagcagatcg
     1021 gcaaggtccg cgcagccgtt gagatagagt ctgagggtcg gcaacgcgat cggctcgtgc
     1081 aaaggcgaaa gcgcctcggt cgcgcagccc agcacccgcg cggcggaata agcgatctgc
     1141 tggcgaaggt cggtccggtt gccgccgggc tgcacgacct cgcgcgacca gagcaaagtg
     1201 tccccgcgat tgtcgaccag cgagaggttc gcccgcaagt cgccttcgcc gcccacgctc
     1261 tcgaccttga agatgaggtc ggccctggtg ccggactccg gctcgaccag ctccagcgca
     1321 tcggccttgg tcgcctggag actgccgagc tgcacgagta ggttcctcgc caaagcctgc
     1381 gcctcagcgc cgcccccgcc ggcaacgacc gtgacggacg ggggagacgc cgccgcgctc
     1441 cacggccgcc acaggaccaa cgccagcagc gccagcgccg ccaccgccag ggcgatcggg
     1501 acaggccgga gaagcgagcg acggctgccc ggcgtatccg ccggcgcttc ctccataggc
     1561 agcgcggcaa gttgctcagc gctcaactga tagacttcga tcggctcgtc gatattcttg
     1621 agctgctgct tgccgatgct ctcgaacggc agccccagcc ggcgcagatc gtcataggcg
     1681 cggcctgaga cgcagatgcc gccgaccggg
//
LOCUS       DASVRK010000151         7602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_91211, whole genome shotgun sequence.
ACCESSION   DASVRK010000151 DASVRK010000000
VERSION     DASVRK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91211"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF589_05940"
     CDS             complement(<1..252)
                     /locus_tag="VF589_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257153.1"
                     /translation="MAMRWMIALLAMLAVTQPDLALAKRPKPQKTEVSKAVGKCVGLV
                     IGGALVGALLGGKKNRGSGALVGAGVGAGACALVMATAKR"
     gene            complement(276..1394)
                     /locus_tag="VF589_05945"
     CDS             complement(276..1394)
                     /locus_tag="VF589_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257154.1"
                     /translation="MLVIDEQSIARSDPAEFRLKNPGVIDAVMAIADPEQAVSLGALA
                     ARERVSLYLAPADGSGPRMLFTGCAPAVSSAEQAELARGTSAAERGAGWFFASGTAEQ
                     IKAQAEEFRTRLLGAVQRVAQAAAEKPGRVAESFEKTSAIRSLKVASGMADPLLGVPR
                     FILIAPAAMRAAAGESNVEAARKAGFAAAERAGLDLGMADVGVALAPGTASREFIGAF
                     LLGSRGRLVAWSSGAPTDLGPAPHAVRNFSGTIQYGAVQFPVRFRLGIDREGRLSSSW
                     IMVMNEQEAATPVSGSAVCDQDGDCRVTGDGRGFSQLWSPRPGGEPEFGTDIPFSGLR
                     YFELSIVRGRATGRIYDPLVGRVGDQSHIPFEMSEMTS"
     gene            complement(1559..2881)
                     /locus_tag="VF589_05950"
     CDS             complement(1559..2881)
                     /locus_tag="VF589_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257155.1"
                     /translation="MSELAQQEAAGRRAGEAAAEVPVEVLDYQKLDLGPNSEAVAWLD
                     VRAETEEVSERHAVTLTGDLEHAPAGLTPNMSAWYARFIAGRRHNAIAAVRNRFEGDQ
                     VRGVPSILLECEADRVAHEAARAKHRAVNEFYSKHAALKHEAEELEQDYERMRAEQGG
                     RKPRMISPWIYFGGLAFVVLLELFLNFESFMSVRFIGSPFLATGITSLVALAIGWSSH
                     LHGTLLRQWDYYFGPHDRLRHFQGWRMLGIGGALLSIALGAVLGARYFYILPKIDQAV
                     LLGIDPPSLLGSLSFMIAGNVIVYLVGATWAYATHDEIPDYPTTKRRAEAKRAEYDRA
                     HRREVSEEVNRLEQKAAAEERKLRQRAVAQEVSPNHVANRQLFQIICSKDQEVCAILL
                     DYRARLSRLHSRVRYRYPDREVLTADPYVELSAGQWAAKDFRLKYHTA"
     gene            complement(2900..3751)
                     /locus_tag="VF589_05955"
     CDS             complement(2900..3751)
                     /locus_tag="VF589_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8257156.1"
                     /translation="MRILDWQDTVCIEHSDRVVRMRRLAEMLPGASRPIFREYVLPRD
                     RLPRAFASGMPLLRVIFPENSFFDTGSSSIRGEAEAALTLIAASLRSEVPDVAVFVAG
                     HADSRGDEDYNYNLSVDRADRVSRRLAQLGIGGVALWRVGFGEAVPLQANDSENGRAV
                     NRRVEFVIGARADPVATWLAEQTPRFCAGRADAVGRCTKPLKRRQFVAEAMSQRTGLV
                     APPRADPPRSVPVKAEHPRNVHVQTQRRQTAPAPAAQRRNIGLAYKARLVLDLNTRKY
                     SIPAPVR"
     gene            complement(4062..5669)
                     /locus_tag="VF589_05960"
     CDS             complement(4062..5669)
                     /locus_tag="VF589_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8257157.1"
                     /translation="MRGLRWAAAVAALAAGGLFQAAPAAPPAAAAMEPVPARTAGEGE
                     GPYRRLVIRGAMLIDGSGAPPRGPVDIVVEGNRISSINGAGTPGLPLPAGREPRDFDR
                     EIDATGQYVMPGFIDTHGHNGDPAKAPNASYGYKLWLAHGVTSVRGVSLYWGQDNRSL
                     ADKKSSAANEIVAPRLFAYLALGDAWSGGEVATPEQARQWVRWAARQGYDGVKLFNSQ
                     PKAVTAAAIDEARRHKLGTVAHLGQSAVAEVNARVAGELGLGTVTHFYGHFEGLLKNG
                     RLPNYPPDYNMLDEQSRFGEIANLADQIVEPGGPEWRAYLEAQLANGVIFDPTFNIYS
                     ASRDLMRARNADWHARYTLPGLYDFFRPSRVNHGSYWYDWTTAREIAWRRFYGPYMRL
                     MNDYKNMGGRVTVGSDPGYIYQTWGFSYVQELEMLQEAGFTPLEAIQAATSNGAHAIY
                     DPKGVEPPIGMVRPRMLADLVIAPENPLHNFKTLYGTGFERLGQDGKVERVGGVRYTI
                     KDGIVYDAKTLLADVERMVEEQKRGSR"
     gene            5750..6112
                     /locus_tag="VF589_05965"
     CDS             5750..6112
                     /locus_tag="VF589_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257158.1"
                     /translation="MGFGYATANTKEIGVLVMRILFLLVAALGITAIAPDADARPRRD
                     RDREQDAAYRARQEGRIMPLPRIERSVRPRMNGADYLGPELDSRSGTYRLKFMRRGRV
                     IWVDVDARTGQVRGQSGE"
     gene            6219..6884
                     /locus_tag="VF589_05970"
     CDS             6219..6884
                     /locus_tag="VF589_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8257159.1"
                     /translation="MRVLIVEDEPNLGRQLRATLEGAGYAVDLATDGEDGHYLGSTES
                     YDAIILDLGLPEIDGLTVLDRWRKEGIKVPVLVLTARDSWSDKVAGLDAGADDYLAKP
                     FQTEELIARLRALIRRASGNSSSELMAGDVRLDTRSGKVTLGGEPVKLTAQEYKLLSY
                     LMHHKGKVVSRTELIEHIYDQDFDRDSNTIEVFVTRIRKKLGPDVITTIRGLGYSLEE
                     PAV"
     gene            6963..>7602
                     /locus_tag="VF589_05975"
     CDS             6963..>7602
                     /locus_tag="VF589_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8257160.1"
                     /translation="MKHDIWLMRRLLPRGRDRDRRSTGSLTRRMIGVAAIWIVVLLGV
                     GGVALDRVLTAAITRNFDEQIGYVLTALIASSEIGPDGEAYLNRPPADQRFLEPYSGL
                     YFQISGEGFDPFRSRSLWDRALAAGGNHRDFEVHSYDSQEFEEEKLRVLERDVRLPGS
                     PVRWRFQVAQSRVGLDEQIAVLRKTMIRSFGVLALGLIAMAALQAFYGLWPLR"
BASE COUNT         1228 a         2639 c         2467 g         1268 t
ORIGIN      
        1 gcgcttggcg gtcgccatca ccaacgcgca ggccccggcg ccgacgcccg cccccaccag
       61 cgccccgctg ccgcgattct tcttgccgcc aagcagcgct ccaaccaagg ccccgccgat
      121 cacaagtccg acgcacttgc cgaccgcctt ggagacttcg gtcttctggg gtttcggccg
      181 cttggccagg gccaggtccg gctgcgtcac ggcgagcatc gccagcagcg cgatcatcca
      241 cctcatcgcc atgtcgcttc cccttctgaa ggttctcaac tcgtcatttc ggacatctcg
      301 aacggtatgt gcgactgatc gccgacgcgg ccgacaagcg ggtcgtagat ccggccggtc
      361 gcgcggccgc gaacgatgga aagttcgaag tagcgaaggc cggaaaaagg tatgtccgtc
      421 ccgaattcgg gctccccgcc tgggcgtggc gaccaaagct gcgaaaatcc gcggccatcg
      481 ccggtcactc ggcagtcgcc gtcctgatcg cagactgcgc tgccgctgac cggcgtcgcc
      541 gcttcctgct cgttcatcac catgatccaa ctgctggaca gacggccctc acggtcgatc
      601 ccgagccgga accgcaccgg gaactggaca gcgccatatt gaatcgtgcc ggagaagttc
      661 cgcacggcgt gtggcgcagg accaaggtcg gtcggggcgc cgctcgacca ggccacgagc
      721 cttccccggg acccgagcag gaatgcgccg atgaattcgc ggctggccgt gccgggggcg
      781 agcgcaacgc cgacgtcagc catacccaga tcgagaccgg ctctttccgc ggcggcgaag
      841 ccggctttgc gtgccgcctc tacattgctc tcccccgccg cggcgcgcat cgccgccggc
      901 gcgatcagga tgaaacgcgg gacgccgagc agcgggtcgg ccatgccgct ggccaccttg
      961 agcgatcgaa ttgccgaagt cttctcgaag ctctcggcca ccctgccagg cttttcggcg
     1021 gccgcctgcg ccacccgctg caccgcgccg agcagcctgg ttcggaactc ctctgcctgt
     1081 gccttgattt gctccgccgt gcctgacgcg aagaaccacc ctgccccacg ttcggcagct
     1141 gacgtgccgc gggccagttc cgcctgctcg gcggaactta ccgccggagc gcagccggtg
     1201 aagagcatcc tcggcccgga gccgtccgcg ggagcgagat agagcgacac acgctcgcgc
     1261 gccgccagag ctccgaggct gaccgcctgc tcgggatccg cgatcgccat cacggcgtcg
     1321 atcacgcccg ggttcttcaa cctgaactcg gcggggtcgg agcgtgcgat gctctgttcg
     1381 tcgatcacca gcacggtctg gcgcagcggc ctggcgagcc ccgcgacctc gcacgcttct
     1441 tcatatttga tcggcccggc aggcgcgccg gaaccgctcc cgccgccgct cccgcaagcg
     1501 gcgacccccg ctgccgcgag cgaggcggcg agcagccgcg cgctggggcg ttccccgctc
     1561 atgccgtgtg gtacttgagc cggaagtcct tagccgccca ttgtccggcg ctaagctcaa
     1621 catagggatc cgcggtcagg acctcgcgat cggggtagcg gtagcggacc ctggaatgaa
     1681 gccgcgacaa ccgcgcccgg taatcgagca gtatcgcgca cacctcctga tccttgctgc
     1741 agatgatctg gaagagctgc cggttggcga cgtggttggg ggacacctcc tgtgcgacgg
     1801 cgcgctggcg aagcttccgc tcctctgctg ccgccttttg ctcgagccgg ttaacctctt
     1861 ccgaaacttc gcggcggtgg gcgcgatcat attccgcccg cttcgcctcg gccctccgct
     1921 tggtggtggg ataatcgggt atctcatcat gggtggcgta ggcccaggtg gcgccgacca
     1981 gataaacgat gacgtttccg gcgatcatga aggaaagcga gcccagcagg ctgggcggat
     2041 cgatcccgag cagaaccgcc tggtcgatct tcgggagtat gtagaagtag cgggcgccga
     2101 gcaccgctcc aagggcgatg gagagcaagg ctccgccaat cccaagcatg cgccagcctt
     2161 ggaagtgccg gagccggtcg tgaggtccga aataatagtc ccactggcga agcagcgtgc
     2221 cgtggagatg gctcgaccaa ccgatcgcaa gcgcgaccag cgaagtgatc ccggttgcca
     2281 ggaaaggcga gccgatgaag cgtacggaca tgaagctttc gaagttgagg aacagctcca
     2341 gcaggacgac gaaggccagc ccgccgaaat agatccaggg gctgatcatg cgcggcttgc
     2401 ggccgccttg ctccgcccgc atgcgctcat agtcctgctc cagttcctcc gcctcgtgct
     2461 tcaacgccgc gtgcttgctg tagaactcgt tgaccgcgcg gtgcttggcc cgcgccgctt
     2521 catgggcgac ccggtcggct tcgcattcca gcaggatcga ggggacaccc ctgacctgat
     2581 cgccctcgaa gcgattccgc accgccgcga tcgcattgtg gcgccgcccc gcgatgaagc
     2641 gtgcatacca ggcgctcatg ttcggcgtca gcccggccgg tgcgtgctcg aggtcccccg
     2701 tcagcgtcac cgcatgccgt tcagaaacct cctcggtttc ggcgcggacg tccagccacg
     2761 ccaccgcctc gctgttcggt ccgaggtcga gcttttgata gtccagaacc tcaacgggaa
     2821 cttcggcggc ggcctcgccc gccctgcgtc ctgccgcctc ctgttgcgcg agctcgctca
     2881 tctttcccct ttccgccggt caccgcaccg gtgccggaat gctgtacttg cgggtgttca
     2941 aatcgaggac gagccgcgcc ttgtaggcca gtccgatatt ccggcgttgc gctgccggcg
     3001 ccggcgccgt ctgccgacgc tgagtctgca catgaacatt ccgggggtgc tccgctttca
     3061 cgggcacgga tcgcggcggg tcggcccgcg ggggcgcaac cagacccgtt cgctggctca
     3121 tcgcctccgc tacgaactgc cttcgcttca gaggcttggt gcagcgcccg accgcatcgg
     3181 cgcggccagc gcagaagcgc ggcgtttgtt cggcgagcca ggtggcgacg gggtcggcgc
     3241 gtgcgccgat cacgaactcg acgcggcggt taactgcgcg gccattctcg ctgtcattgg
     3301 cttgcagcgg aacggcctcg ccaaagccga cacgccacag cgccacgccg ccgatgccga
     3361 gttgcgcgag ccgccgcgac acgcgatcgg cgcgatccac cgaaagattg taattataat
     3421 cttcgtcccc ccggctgtcg gcgtgcccgg cgacgaatac agcgacgtcc ggaacctcgc
     3481 tgcgcaggct ggctgcgatc agcgtcaagg cggcctccgc ctccccccgg atgctgctgc
     3541 tgccggtgtc gaagaaggaa ttctccggga agatgacacg cagcaagggc atcccggaag
     3601 cgaaggcgcg tggcagacga tcgcgcggca gcacatattc gcgaaagatc ggcctcgaag
     3661 cgcctggcag catctcagcc agccggcgca ttcgcacaac ccggtccgaa tgctcgatgc
     3721 acaccgtatc ttgccagtcg agtatccgca ccgcgggccg cccgggaaga gcgggcgctg
     3781 cccggccaat cgccgccccg tctttgcaga gcggctcagc cgcggcagtg gcggcgccgg
     3841 ctcccgcata aagcaggagg caatgcgcga ctcgcttgac cccccagatc atcacgcgag
     3901 cataggctgc tcgctaggcc tcgccgcccc tcgtttgatc cgaatccacg gcaagcatct
     3961 ccacaatgga gctaacgagc aagcctgctc gcaactttcg ggaaaccggt ccggcgttca
     4021 gccgagcgtc ggagccccgt ggaactctcc gcaccacgct atcagcggct accccgcttc
     4081 tgctcctcca ccatccgctc gacatcggcg agcagggttt tggcgtcgta gacgatgccg
     4141 tccttgatcg tatagcggac gccgccgacc cgctcgacct tgccgtcctg gccgagccgc
     4201 tcgaagccgg tgccgtagag cgtcttgaaa ttgtggagcg ggttttcggg cgcgatcacc
     4261 aggtcggcga gcatgcgcgg tcgcaccatg ccgatcggcg gctcgacgcc cttgggatcg
     4321 tagatggcat gggcgccgtt gctggtggcg gcctggatcg cctccaaggg cgtgaagccc
     4381 gcctcctgca gcatctccag ctcctggacg taggagaagc cccaggtctg gtagatatag
     4441 ccggggtccg agccgacggt gacccggccg cccatattct tatagtcgtt catcagccgc
     4501 atgtaggggc cgtagaagcg ccgccaggcg atctcgcgcg cggtcgtcca atcgtaccaa
     4561 taggagccgt ggttgacccg gctcgggcgg aagaaatcgt agaggccggg cagggtgtag
     4621 cgcgcgtgcc aatcggcgtt gcgcgcgcgc atcaggtcgc gcgaggccga gtagatgttg
     4681 aaggtcgggt cgaagatcac cccattggcc agctgcgcct cgagataggc gcgccattcg
     4741 gggccgccgg gctcgacgat ctgatcggcg aggttggcga tctcgccgaa ccgcgactgc
     4801 tcgtcgagca tattgtaatc gggcgggtaa ttgggcagcc ggccattctt cagcagccct
     4861 tcgaaatggc cgtaaaagtg ggtgacggtg ccgagcccga gctcgcccgc gacccgcgca
     4921 ttgacctcgg cgacggcgct ctggccgaga tgcgcgaccg tgccgagctt gtggcgccgg
     4981 gcctcgtcga tcgcggcggc ggtcaccgcc ttgggttggc tgttgaacag cttgacgccg
     5041 tcatagccct gccgcgccgc ccatcgcacc cactggcgcg cctgctcggg cgtggcgacc
     5101 tcgccgccgc tccaggcgtc gccgagcgcg agataggcga acagccgcgg cgccacgatc
     5161 tcattggccg cgctgctttt tttatcggcg agcgacctgt tgtcctgacc ccagtagagc
     5221 gagacgccgc gcacgctggt gacgccgtgg gcgagccaga gcttgtagcc gtagctcgcg
     5281 ttgggcgcct tggccgggtc gccattatgg ccgtgcgtgt cgatgaagcc cggcatcaca
     5341 tactggcccg tggcgtcgat ctcgcggtcg aagtcgcgcg gctcgcggcc ggcggggagc
     5401 ggcaggccgg gcgttccggc gccgttgatg ctgctgatcc ggttgccctc gacgacgata
     5461 tcgaccggcc cgcgcggcgg cgcgccgctg ccgtcgatca gcatcgcgcc gcgaatcacg
     5521 agccggcggt agggcccctc gccctctccg gccgtccgcg ccggcaccgg ctccatcgcc
     5581 gcagcggccg gaggcgcggc gggcgccgcc tgaaaaagcc caccggcggc cagcgccgcc
     5641 acggccgccg cccatcttaa cccgcgcata tcccctcgct ttttccgtgg cttgcctgca
     5701 acagccgcga agctgtcgtt ccgatgacga accattcatg gcttattcaa tgggctttgg
     5761 ctatgccacg gccaacacga aggaaattgg tgttttggta atgcgtatcc tgttcctctt
     5821 ggtagccgcc ctcggcataa cggccatcgc gccggatgct gacgcgcgcc cgcgccggga
     5881 ccgggaccgg gagcaggacg ccgcctaccg ggcgcggcag gaggggcgga taatgccgct
     5941 cccccgcatc gaacgcagcg tccgcccgcg catgaacggc gccgactatc tcggacctga
     6001 actggactcg cgctcgggca cctatcgcct gaaattcatg cgccgcggcc gggtgatctg
     6061 ggtcgacgtc gacgcgcgca ccggccaggt caggggccag tccggcgaat aagccggctg
     6121 gcgccgccga cccgccacga aacacccgcc acgaaacaat tggctttcgc accggttggg
     6181 cggctagatt gtccgctgaa caggtcggag aaacatcaat gcgggttctg atcgtcgagg
     6241 acgagcccaa tcttggccgc cagctgaggg cgaccctgga aggcgccggc tatgccgtcg
     6301 atctcgccac cgatggcgag gacggccatt atctcggctc gaccgaaagc tacgacgcga
     6361 tcatcctcga cctcggcctg ccggagattg acggcctgac cgtgctcgac cgctggcgca
     6421 aggaggggat caaggtcccc gtgctggtgc tgacggcgcg cgacagctgg tccgacaagg
     6481 tggcggggct cgacgccggc gccgacgatt atctcgccaa gccgttccag accgaggagc
     6541 tgatcgcgcg gctgcgcgcg ctgatccgcc gcgcctcggg caattcgagc tccgagctga
     6601 tggccggcga cgtccggctc gacacaaggt cgggcaaggt cacgttgggc ggcgagccgg
     6661 tcaagcttac cgcgcaggaa tataaattgc tctcctacct catgcaccac aagggcaagg
     6721 tggtcagccg caccgagctg atcgagcata tttacgatca ggatttcgac cgcgattcca
     6781 acaccatcga agtgttcgtg acgcgcatcc gcaagaagct cggtcccgac gtcatcacca
     6841 ccatccgcgg cctcggctac tcgctggagg agcccgccgt ttgaggatgg cgccggccat
     6901 cccaatcttg actccggcct ggtcgagcgg atcggccgcc gcagaggctc ggacatgacg
     6961 gcatgaagca cgacatctgg ctgatgcgcc ggctgctgcc gcgcggccgc gaccgcgacc
     7021 gccgctcgac cggatcgctg acccggcgca tgatcggcgt cgcggcgatc tggatcgtgg
     7081 tgctgctcgg cgtcggcggg gtcgccctcg accgcgttct gaccgccgcc atcacgcgca
     7141 atttcgacga gcagatcggc tacgtcctca ccgcccttat cgcctcctcc gagatcggcc
     7201 ccgacggcga ggcctatctc aaccgtccgc ccgccgacca gcgcttcctc gaaccttatt
     7261 ccggcctcta cttccagatc agcggcgagg gcttcgaccc gttccgctcg cgctcgctgt
     7321 gggaccgggc cttggcggcc ggcggcaacc accgcgattt cgaagtgcac agctatgaca
     7381 gccaggagtt cgaggaggag aaattgcgcg tcctcgaacg cgacgtccgc ctgccgggct
     7441 cgccggtgcg ctggcgcttc caggtcgcgc agagccgggt cggcctcgac gagcagatcg
     7501 cggtgctgcg caagaccatg atccgctcgt tcggggtgct cgcattgggg ctgatcgcga
     7561 tggcggcgct gcaggccttt tacggcttgt ggccgctgcg cc
//
LOCUS       DASVRK010000152         9065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_526425, whole genome shotgun sequence.
ACCESSION   DASVRK010000152 DASVRK010000000
VERSION     DASVRK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526425"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VF589_05980"
     CDS             <1..789
                     /locus_tag="VF589_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8257161.1"
                     /translation="TVDRIVKVIAGENAAVTELLARIYGAVTSAGVFRARSIKTAEAA
                     KVIENAQRDINIAFMNEITQIFAKLGLSTLDVLDAAGTKWNFLKFRPGLVGGHCIGVD
                     PYYLSHCAQSLGHLPRVILAGRSINDGMGSWIADCIHSRRGSRTGSVLILGVTFKEDV
                     PDLRNSKVVDLANRLRWLGHEVTLHDPLADPAAARHEYGQDLDPAALERRYDVVLPAV
                     PHRSYRAMAADAVAALAARDGLVADLHGIWRDLEFADTLDRWSL"
     gene            complement(831..1829)
                     /locus_tag="VF589_05985"
     CDS             complement(831..1829)
                     /locus_tag="VF589_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8257162.1"
                     /translation="MSILVTGAAGFIGFHVAHHLLARGETVVGIDNVNPYYSVALKRD
                     RLTELAARHGDRFRFAELDFADRPALEAALDGTEIAGAVHLGAQAGVRYSLENPLAYA
                     HSNLIGHLNMLELARARRLPHLVYASSSSVYGGNATLPFRVEDRVDAPLSLYAATKRA
                     DELMSETYAHLYRLPQTGLRFFTVYGPWGRPDMAMWLFTQAILEGRPIDVYGEGNMRR
                     DFTYIDDIVAGVVAALDHPPADDGEEKAGGSRSPHRLYNIGNHRSEELDRMIGLIEAA
                     CGRKAERRMMPIQPGDVRDTYADITAIERDLGFAPATCIDEGIPRFVDWYRRYHGV"
     gene            complement(1862..2674)
                     /locus_tag="VF589_05990"
     CDS             complement(1862..2674)
                     /locus_tag="VF589_05990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8257163.1"
                     /translation="MSLWSKVFKRKGNDEPPAAFGLEGARAYAIGDIHGRLDLLNNLL
                     DRIEQDIRAREPRRNVIVFLGDYVDRGPESAGVIERLRTFRPEHAEAVFLAGNHEEFF
                     LRILHGDTHSLAQWLKFGGAECVESYGIEVPQLLRLPRSEALRLLQASVPAAHVEFLY
                     GLEDSYGFGQYLFVHAGIRPGIALEEQKRSDLRWIRDPFLSFDGSHGCVVVHGHTIVE
                     EVQERRNRIGIDTGAYRSGLLTAVAIEDDRRWYLAGQLSGAEAIPAADPPAH"
     gene            3033..3290
                     /locus_tag="VF589_05995"
     CDS             3033..3290
                     /locus_tag="VF589_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257164.1"
                     /translation="MPSKKVSALAAAALLLASSAAVAQTRSLPVFASTAALLQTDDEG
                     DDDDGAGAPYTGILIALVIGAAALLIATGGGGGGDDAVSPD"
     gene            3602..5368
                     /locus_tag="VF589_06000"
     CDS             3602..5368
                     /locus_tag="VF589_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX8257165.1"
                     /translation="MLAGAAAAAWLPAPAFAQEVPAGALPPTREEIERAPLRPTVEAA
                     PRLTVEGDVERAPCALAGDAYRNLTFTVSSVAFEDLKGVAPADLAPAYAEYIGTTQPV
                     AVICEIRDRAATILREAGYIAAVEVPEQRIAEGAIRFQVLMARLVAIRVRGNAANAER
                     TIAGYLERLTEEEVFNRFHAERYLLLAGDLPGYDVRLALRSAGAGRGEVIGEVAVLRS
                     PGQIDLNVQNFGSRSLGRWGGLLRGQFYGLTGLGDRTTVALFSTADFEEQRTLQLGHD
                     FRVGSEGLTLSGQFTYAWANPDLDDDRFDIKSTTMLATVEAAFPFIRTQAQTLRGSFG
                     LDIIDQDVRLVDVTDLTRDRLRVLFSRVDYEAVDTDSLSRIGGFSAAEPRWRFAGGLE
                     LRHGVDIFGGSEDCRNGGCKAPAVPISRLEGDPTGTVLRFESFGEYRPVPNITFALGV
                     RGQYSNDPLLSFEEFSAGNYTVGRGYDPGALLGDRGIGVQAELRYGTIVPAALDRFSF
                     QPYVFFDAAWVGNEDRGNRLDVRQNLSSVGGGIRALYGDRVQLDVALAVPLEAAGLLT
                     KERPDPRLLVSLTTRLWPWRSR"
     gene            5365..>9065
                     /locus_tag="VF589_06005"
     CDS             5365..>9065
                     /locus_tag="VF589_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257166.1"
                     /translation="MIDPVPPASPLWLRARRSSLLLSCALGTVLAVAGPSGRALAQAF
                     QGDPSVVSGQVGFDRGTAGRDVITVGSPTAVVNWDPNDTSGGGRIDFLPQGHTAIFQN
                     NPQSTSDFFILNRIVPADPSRAVAFNGTVISQLQTNAGNVAGGTVAFYSPGGILIGST
                     ATFDVGSLLLTTLDPVRNANGGIIGNGSSLQLRGPDGSSSAITTEAGSTIRAVSEGSY
                     VAMAAPRIQHGGAVRVNGSAGYIAAEAVDLTINDGLFDISVQTGTTQSNALNHTGSTG
                     GPASGGAGDNHNIYMVAVAKNQAITALLSGSVGFDSATSATVENGAIILSAGHNVRGT
                     RILADSNGNGVNSVEANFHVNGGAYSSDVTGRATSDFFVAGQSASTDFAGDVSIQAGR
                     GAHIAGRNGQVVTILGNASVNADRLYLDAGSAGVGATAGEALLYADGGGTLDIRGDAS
                     VSADATAGGSISAPALATGGQARIFGSDGTVTIGGAATLSAVANRAFPAASGVTGPAG
                     QGGTAEIFVNSGGSVAISGAARLDATGYGAANGAAGGVGASGRGGNASVFGGGGEIAI
                     GGIAFLTADGVGGDGRSGGTGQGGSAGISATEGSIELADAAIIRARGTGGNASAGFGG
                     TGGAGTGGVAFVEARQTATEGGTITGQDVLLDVSGFGGRGGAGDGSAIAAGAGGAGSG
                     GTFTGTTGSGGAFVLAQADRGRLSLGEVILATQGIGGAGGSGGAGQAGGAGGTGTGGS
                     GQTGTFLGDGDGLTLGGSAAFGNVSIDASGLGGAGGSGAAGSGDGGDAAGGAAAHIGR
                     GTAVTAGRVDLFATARGGAGAAGGNAVGGAAEALADGGSLAMGDLAVDAGAVGGAGAA
                     GGGATGGTAQITAHSGGRIDVEGAINATSSGFGGESSGAQSAGGAGQGGATSMVLDNG
                     RLAVGGMTNLFADGIGGAGSRGGDGSGGSAEIHIQSGGGTLTAGSPTLVQAAGNGGDA
                     SGGGAGGRGAGGTASLTVAPGAEASAVVAMADTYVNAAATGGNGAGAGAGGDGEGGVA
                     RVALTGGSAEFGELRVFTTTRGGQGGAAAHGNGGSGGSATAGDSSLIVGGQLSTSSHL
                     VYSRAFGGAGGAGSVRGAGGDATGGMALIDVNTGGSLASGGLADINATATGGAGGIGG
                     NATGGSATFALAGGQATLQDATVDASAFGGAGNSGAGGEAEGGSAAINASGGTLTTTG
                     PVQVLASASGGAGAGAGAGGRGEGGAAPAR"
BASE COUNT         1289 a         3205 c         3194 g         1377 t
ORIGIN      
        1 accgtcgatc ggatcgtcaa ggtcatcgcc ggcgagaacg cggcggtgac ggagcttctc
       61 gcccggattt acggcgcagt gaccagcgcg ggcgtcttcc gtgccaggtc gatcaagacc
      121 gccgaggccg ccaaggtgat cgagaacgcc cagcgcgaca tcaacatcgc cttcatgaac
      181 gagatcaccc agattttcgc caagctcggc ctctccacgc tcgacgtgct cgacgccgcc
      241 ggcaccaaat ggaatttcct gaagttccgc cccggtctgg tcggcggcca ctgcatcggc
      301 gtcgatcctt attatctcag ccattgcgcc caaagcctcg gccatctgcc gcgcgtgatc
      361 ctcgccggcc gctcgatcaa cgacggcatg gggagctgga tcgccgactg catccacagc
      421 cggcgcggca gccgcaccgg cagcgtcctg atcctcggcg tgacgttcaa ggaggacgtc
      481 cccgatctgc gcaattccaa agtggtggat ctcgccaacc ggctgcgctg gctcggccat
      541 gaggtcaccc tccacgatcc gctcgccgat ccggccgccg cccgccacga atatggccag
      601 gatctcgatc ccgccgcgct cgaacgccgc tacgacgtcg tgctgccggc cgtcccgcac
      661 cggagctacc gggcgatggc ggcggatgcg gtcgccgcgc tggcggccag ggacgggctg
      721 gtcgccgatc ttcacggcat ctggcgcgac ctggaattcg ccgacaccct cgaccgctgg
      781 tcgctataag ctagcctcgg cggggccgtc cgcgttcggg ctccgcggcc tcagacgccg
      841 tgatagcgcc tgtaccagtc gacgaagcgc ggaatgccct cgtcgatgca ggtggcgggg
      901 gcgaagccga ggtcgcgctc gatcgcggta atgtcggcat aggtgtcgcg cacgtcgccc
      961 ggctggatcg gcatcatccg ccgctccgcc ttgcggccgc acgccgcctc gatgaggccg
     1021 atcatgcggt cgagctcttc gctcctgtga ttgccgatat tgtagagccg gtgcggcgag
     1081 cggctgccgc cggccttttc ctcgccgtcg tcggcgggcg ggtggtcgag cgccgccacc
     1141 accccggcga cgatgtcgtc gatataggtg aagtcgcgcc gcatattgcc ttcgccatag
     1201 acgtcgatcg ggcggccttc gaggatcgcc tgggtgaaca gccacatcgc catgtccggc
     1261 cggccccagg gcccgtagac ggtgaagaag cgcaggcccg tctgcggcag ccggtagaga
     1321 tgcgcatagg tctcgctcat cagctcgtcg gcgcgcttgg tggcggcgta gagcgacagc
     1381 ggcgcgtcga cccggtcctc cacccggaac ggcagggtcg cgttgccgcc atagacggac
     1441 gaggaggagg catagacgag gtggggcagg cggcgcgcgc gcgccagctc cagcatgttg
     1501 aggtgcccga tcaggttgga atgggcataa gcaagcgggt tttcgagcga gtagcgcacc
     1561 ccggcctggg cgccgagatg gaccgcgccc gcaatctcgg tgccgtccaa ggcggcttcg
     1621 agcgcgggac ggtcggcgaa gtcgagctcg gcgaaacgga agcggtcgcc atgcctcgcc
     1681 gccagctccg tcaggcggtc gcgcttcagg gcgacgctat aataagggtt cacgttgtcg
     1741 atcccgacca cggtctcgcc gcgggcgagc agatggtggg cgacgtggaa gccgatgaac
     1801 ccggccgcgc cggtgaccag tatgctcatc cggccttcat agtcggcgct ggtaaattaa
     1861 ttcaatgcgc cgggggatca gcggcgggta tcgcctcggc gccgctgagt tgcccggcca
     1921 ggtaccagcg gcggtcgtcc tcgatcgcca cagcggtgag caagccgctg cgataggcgc
     1981 cggtgtcgat cccgatccgg ttgcggcgct cctggacttc ctcgacgatg gtatggccgt
     2041 ggacgacgac gcagccatgg ctgccgtcga agctgaggaa cgggtcgcgg atccagcgca
     2101 ggtccgaccg tttctgttcc tcgagcgcga tgccgggccg gatgccggca tggacgaaca
     2161 gatattggcc gaagccgtaa ctgtcctcga gcccataaag aaactcgaca tgggcggcgg
     2221 gaacgctggc ctgcagcagg cgcagcgcct cgctgcgcgg cagccggagc agttgcggca
     2281 cttcgatgcc gtagctttcg acgcactccg cgccgccgaa cttcagccac tgcgccagcg
     2341 aatgggtgtc gccatggagg atccggagga agaattcctc gtgattgccg gccaggaaca
     2401 ccgcctccgc atgttcggga cggaaggttc tcagccgctc gatcacgccg gccgactccg
     2461 ggccgcgatc gacatagtcg cccaggaaga cgatcacgtt gcggcgcggc tcgcgcgccc
     2521 ggatatcctg ctcgatccgg tcgagcaggt tgttcaacag gtcgagccgg ccatggatgt
     2581 cgccgatcgc ataggcgcgc gcgccctcca gcccgaacgc ggcggggggc tcgtcattgc
     2641 ctttgcgctt gaataccttg ctccagaggc tcacactcgt acccgtccat ttcactcgcc
     2701 gccgccatcg gctcggccgc cggcggggcg cgtggcggcc tcgctccggc aaatgctggc
     2761 ggtcctagtc tgccagcggt ggccgcgcaa tcgtcaaacg ggttgccgaa aaggctgtgt
     2821 cggcaactcc ttggcggagg gtggccgcgc ctcgtccgca gccgcgcctg gcgcggggga
     2881 ggcggcgggc acggccccgg agcggttccg ggggcgttac ttcttgagga gcggcggccg
     2941 tcgttaactc ttaagtgctg gccaagcgct atttccggcg ataggttagc attgccaatc
     3001 tgattctgct ttggtcgaag gagttaccta taatgccctc caagaaagtt tctgcccttg
     3061 ctgcagccgc gctgctgctc gcctcgagcg cggcggtcgc ccagacgcgc agcctgccgg
     3121 tcttcgccag cacggccgcc ctgttgcaga ccgatgacga aggcgacgat gatgacggcg
     3181 ccggcgctcc ctacacgggc atcctgatcg cgctcgtgat cggcgcggcc gcgctcctga
     3241 tcgccaccgg cggcggcggc ggcggcgacg acgcggtcag ccccgactaa gctgtttcgg
     3301 cgcgctccgg gggtccggag cgcgccatca tgtctgcact catccagcgc ggcgggccca
     3361 ttgctcgccg ctctttgcat gcctgcgcct ggccgcttcc gggcctgcct gccctgcttc
     3421 ccctacatta gactatccat ttgtcgcaaa atgagtctat gaaaccggac tcgcgcataa
     3481 ggtcgcgggg cgggaagagg gggctcttcg aacagtgggt tgggggacaa gtcaccatag
     3541 cgccagccgc cggccggctt cggcgggccg cgcgcgccca tccggcgggc tccgcaccgc
     3601 catgctggcg ggcgccgccg cggccgcctg gctcccggca cccgccttcg cgcaggaggt
     3661 tccggccgga gccctcccgc ccacccgcga ggagatcgag cgcgctccgc tccgcccaac
     3721 ggtcgaggcg gcgccgcgac tcacggtcga gggcgatgtc gagcgcgcgc cctgcgccct
     3781 tgccggcgat gcctatcgca acctgacctt caccgtcagc tcggtcgcct tcgaggacct
     3841 caagggcgtt gcgccggccg acctcgcgcc ggcttatgcc gaatatatcg gcaccaccca
     3901 gccggtggcg gtgatctgcg aaatccgcga ccgcgccgcc accatcctgc gcgaggccgg
     3961 ctatatcgcc gccgtcgagg tgcccgagca gcggatcgcc gaaggcgcga tacgtttcca
     4021 ggtgctgatg gcgcggctgg tggcgatccg ggtgcgtggc aatgccgcca atgccgagcg
     4081 caccatcgcc ggctatctcg agcgcctgac cgaagaggag gtgttcaacc gcttccacgc
     4141 cgagcgctac ctgctcctcg ccggcgatct tcccggctat gacgtgcggc tggcgcttcg
     4201 ctccgccggc gccggccgcg gcgaggtgat cggcgaggtc gccgtgctgc gcagccccgg
     4261 ccagatcgat ctcaacgtcc agaatttcgg ctcgcgcagc ctcggccgct ggggcgggtt
     4321 gctgcgcggc cagttttacg ggctcaccgg cctcggcgac cgcaccaccg tcgccctctt
     4381 cagcaccgcg gatttcgagg aacagcggac gcttcagctc ggccacgatt tccgcgtcgg
     4441 cagcgagggc ctgacgctgt ccggccagtt cacctacgcc tgggcgaatc ccgacctcga
     4501 cgacgaccgc ttcgacatca agtcgaccac catgctggca acggtcgagg cggccttccc
     4561 cttcatccgc acccaggcgc agacgctgcg cggaagcttc ggcctcgaca tcatcgacca
     4621 ggatgtccgc ctcgtcgacg tcaccgacct tacccgcgac cggctgcggg tgctcttctc
     4681 gcgcgtcgac tacgaggcgg tggacaccga cagcctttcc cgcatcggcg gcttctcggc
     4741 ggccgagccg cgctggcgct tcgcgggcgg gctcgagctc cgtcacggcg tcgacatatt
     4801 cggcggcagc gaggattgcc gcaacggcgg ctgcaaagcc cccgctgtcc cgatcagccg
     4861 gctcgaaggc gatccgacgg ggacggtcct gcgcttcgaa agcttcggcg aatatcgccc
     4921 ggtgcccaat atcaccttcg cgctcggcgt ccgcgggcaa tattccaacg acccgctgtt
     4981 gagcttcgag gaattctccg ccggcaacta cacggtcggc cgcggctacg acccgggcgc
     5041 gctgctcggg gaccgcggca tcggcgtgca ggcggagctt cgctacggca ccatcgtccc
     5101 ggccgcgctc gatcgcttct ctttccagcc ctacgtgttc ttcgacgcgg cctgggtcgg
     5161 caacgaggat cgcggcaacc gcctcgacgt gcgccagaat ttgagctcgg tcggcggcgg
     5221 catacgcgcc ctctacggcg atcgcgtcca gctcgacgtg gcgctggccg ttccgctcga
     5281 ggcggcgggg ctgctcacga aggagcggcc cgacccccgc ttgctggtgt cgctcaccac
     5341 gcgcctttgg ccatggagat cccgatgatc gaccctgtcc cgcccgcatc gcctttgtgg
     5401 ttgcgcgccc ggcgcagttc cctgctcctg tcctgcgcgc ttggcaccgt gcttgccgtc
     5461 gccggcccgt ccgggcgggc gctcgcccag gcgttccaag gcgatccttc ggtggtgtcg
     5521 ggccaggttg gcttcgatcg cggcaccgcc ggtcgcgacg tcatcacggt cggctcgccg
     5581 accgcggtgg tcaattggga tccgaacgac accagcggcg gcggccggat cgacttcctg
     5641 ccgcagggcc acactgcgat cttccagaac aacccgcagt cgacctccga cttcttcatc
     5701 ctgaaccgga tagttcccgc cgatccgagc cgggcggtcg ccttcaacgg caccgtcatc
     5761 agccagctcc agaccaatgc cgggaacgtc gccggcggca ctgtcgcctt ttacagcccc
     5821 ggcgggatcc tgatcggctc gaccgcgacc ttcgacgtcg gcagcctgtt gctgactacg
     5881 ctcgatccgg tccgcaacgc caatggcggg atcatcggca acggctccag cctgcagctg
     5941 cgcgggcccg acggcagcag ctccgcgatt accaccgagg ccggatcgac catccgggcg
     6001 gtttcggaag gcagctacgt cgccatggcg gcgccgcgca tccaacatgg cggcgcggtg
     6061 cgggtcaacg gatcggccgg ctatatcgcc gccgaagcgg tcgacctcac catcaatgac
     6121 ggcctgttcg acatcagtgt ccagaccggc accacgcaat ccaatgcgct caatcacacc
     6181 ggctcgaccg gcggcccggc cagcggtggc gccggcgaca atcacaatat ctatatggtc
     6241 gcggtcgcca agaaccaggc gatcaccgcc cttctgagcg gctcggtcgg cttcgattcg
     6301 gcgacttcgg cgacggtcga gaatggcgcc atcatattgt cggccgggca caatgtccgc
     6361 ggcacccgca ttctcgccga ttcgaacggc aacggcgtca acagcgtgga ggcgaacttc
     6421 cacgtcaatg gcggcgccta cagctccgac gtcaccggcc gcgccaccag cgacttcttc
     6481 gtcgccggcc agtcggcgag caccgatttc gccggcgacg tctcgatcca ggccggccgc
     6541 ggcgcccaca tcgccggccg caacggccag gtcgtgacga tcctcggcaa cgccagcgtc
     6601 aacgccgatc ggctctatct cgacgccggt agcgccggcg tcggcgccac cgcgggcgag
     6661 gccctgctct acgccgacgg cggcggcacg ctcgacatca ggggcgatgc ttcggtttcc
     6721 gcggacgcga cagcgggcgg tagcatcagc gcccccgcgc tggcgacggg tggccaagcg
     6781 cgcatcttcg gcagcgatgg cacggtcacc attggcggcg ccgcgacgct cagcgcggtc
     6841 gccaaccgag ctttcccggc tgccagcggg gtcaccggtc ccgccggcca gggcggcaca
     6901 gccgagattt tcgttaactc cggcggcagc gtggcgatct ccggcgcggc gagactggat
     6961 gcgaccggct atggcgctgc caatggcgcc gccggcggtg tgggggcttc gggccggggc
     7021 ggcaacgcct cggtgttcgg cggcggcggc gagatcgcca tcggcgggat cgctttcctg
     7081 acagcggatg gagtcggcgg cgatggccgg tccgggggca ccggtcaggg cggttcggcc
     7141 gggatcagcg ccaccgaggg cagcatcgag cttgctgacg ccgccattat ccgcgctcgc
     7201 ggcaccggcg ggaatgcctc ggccggcttc ggcggcacgg gcggggccgg cacgggcggg
     7261 gtcgccttcg tcgaggcccg ccagaccgcc accgagggcg gcaccatcac ggggcaggac
     7321 gtgttgctcg atgtctccgg cttcggcggc cgcggcggcg ccggcgacgg cagcgcgatt
     7381 gcggcgggcg cgggcggcgc cggcagcggc ggcaccttta ccgggactac cggatcgggc
     7441 ggcgccttcg tgctcgcgca ggcggaccgc ggcaggctca gtctcggcga agtcattttg
     7501 gcgacgcagg gcattggcgg cgccggcggc agcggcggcg ccggtcaggc gggcggggcc
     7561 ggcggcacgg gtaccggcgg ctccgggcag accgggacgt tcctcggcga cggcgacggt
     7621 ctcacgctcg gcggcagcgc tgccttcggc aacgtgtcga tcgacgcgag cggccttggc
     7681 ggcgcgggcg gcagcggcgc agccggatcc ggcgacggcg gcgacgcggc gggcggcgcc
     7741 gccgctcata tcggccgcgg gacggcggtt acggccgggc gggtcgatct cttcgcgacc
     7801 gcccgcggcg gggcaggcgc ggccggaggc aatgccgtcg gcggcgccgc cgaagcgctg
     7861 gccgatggcg gttcgctcgc gatgggcgat ctcgcggtgg acgccggagc cgtcggcggc
     7921 gctggcgcgg caggcggcgg cgccaccgga ggcaccgcgc agatcaccgc gcattccggc
     7981 ggccgtatcg atgtcgaggg cgcgatcaat gccaccagct ccgggttcgg cggcgagtcc
     8041 tcgggcgcgc aatccgccgg cggcgccggc cagggcggcg ccacctcgat ggtgctcgac
     8101 aatggccggc tcgcggtcgg cgggatgacc aacctttttg ccgacggcat cggcggcgcc
     8161 ggttcgcgcg gcggcgacgg tagcggcggc agcgccgaaa tccatattca aagcggcggc
     8221 ggcacgctca cggccggtag cccgaccctg gtccaggcgg ccggcaacgg cggcgatgcg
     8281 agcggcggcg gcgccggcgg caggggcgcc ggcggcaccg cttcgctgac cgtcgcgccc
     8341 ggcgcggagg cttcggcggt cgtcgccatg gcggacactt atgtgaacgc cgcggcgacc
     8401 ggcggcaatg gcgcgggcgc cggcgccggc ggggatggcg agggcggcgt cgcgcgcgtc
     8461 gcgctgacgg gcggctcggc tgagttcggc gagctgcgcg tctttacgac cacccgcggc
     8521 ggccagggcg gagcggcggc ccacggcaat ggcggtagcg gcggatcggc cacggccggc
     8581 gattcgtcgc tgatcgtggg cgggcagctg tcgaccagct cccacctcgt ctattcgcgc
     8641 gctttcgggg gcgccggcgg cgccggttcg gtccgcggcg ccggcggcga tgccacgggc
     8701 ggcatggccc tgatcgacgt caataccggc ggcagcctcg cgtcgggtgg gctcgccgac
     8761 atcaacgcga ccgccaccgg cggcgccggc ggcatcggcg gcaatgccac cggcggcagc
     8821 gccaccttcg ccttggcggg cgggcaggcg acgctccagg atgccaccgt cgacgcctcg
     8881 gcattcggcg gcgccggtaa tagcggcgcc ggcggcgagg ccgaaggcgg cagcgcggcg
     8941 atcaacgcct cgggcggcac tttgacgacg accggcccag tccaggtgct cgccagtgcc
     9001 tccggcggcg ccggcgccgg cgcaggcgcc ggcggacgcg gcgagggcgg cgccgccccg
     9061 gcgcg
//
LOCUS       DASVRK010000153         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_526457, whole genome shotgun sequence.
ACCESSION   DASVRK010000153 DASVRK010000000
VERSION     DASVRK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526457"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..499
                     /gene="hfq"
                     /locus_tag="VF589_06010"
     CDS             11..499
                     /gene="hfq"
                     /locus_tag="VF589_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX8257167.1"
                     /translation="MADKVNNLQDMFLNGLRRSKTPVTMFLVKGVKLQGIITWFDNFS
                     VLLRRDGQAQLIYKHAISTIMPAQPIDMAEIERAFAAQADKKKPALLQEVFLSAVRRS
                     GEPVTMFLVNGVMLQGEIAAFDLFCMLLQREGLSQLVYKHAISTVQPEHPISFIEADQ
                     EE"
     gene            504..1808
                     /gene="hflX"
                     /locus_tag="VF589_06015"
     CDS             504..1808
                     /gene="hflX"
                     /locus_tag="VF589_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX8257168.1"
                     /translation="MSVFDRSSEADVSRGARAVVALPEMGGAARRDPAARLDEAAGLA
                     EAIGVVVAEKLSFKVRAPKAATLFGSGQVETIAAAAEQAEAELIIIDAPITPIQQKNL
                     ESATKAKVIDRTGLILEIFGERAATAEGRLQVELAHLDYQAGRLVRSWTHLERQRGGF
                     GFLGGPGETQIEADRRLIRDRMAKLRKELEQVTRTRSLHRARRQRAPWPVIALVGYTN
                     AGKSTLFNRLANADVMAENLLFATLDPTMREIAVPGFDKAILSDTVGFISDLPTQLVA
                     AFRATLEEVISADLILHVRDISHPDSDAQRLDVEQVLTEIGAIGEGASPLIEAWNKID
                     LLGAEEAASVGAEAARREDVVPISALTGERVDALIAAAAERLREGSRVRKVTLPAGAG
                     EAIAWLHANGEVIEQRSEGLETEFEVRISDADWARFQTRHLA"
     gene            complement(1805..2527)
                     /gene="mazG"
                     /locus_tag="VF589_06020"
     CDS             complement(1805..2527)
                     /gene="mazG"
                     /locus_tag="VF589_06020"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX8257169.1"
                     /translation="MVAIMARLRDPDTGCAWDRVQTFATIAPYTVEEAYEVADAIERG
                     DMADLKDELGDLQLQIVYHARIAEELGHFALGDVIAAICAKMIRRHPHIFAAAGESPG
                     WEALKAAERGAREDPSALAHVALALPALKRAEKLQTRAARVGFDWPDPSGPRAKIDEE
                     LAELDHAAGDEERAAELGDLLFSVVNYARHLGVDPEAALRGSAARFEARFRKVEALAE
                     RPLAEMDIDALEALWQEAKRRL"
BASE COUNT          459 a          904 c          985 g          428 t
ORIGIN      
        1 agagcagaat atggccgaca aggtcaacaa cctccaggac atgtttctga acggcctcag
       61 gcgctccaag accccggtca ccatgttcct ggtcaagggc gtaaagcttc aggggattat
      121 cacctggttc gacaatttct ccgtgctcct gcgccgcgac gggcaggcgc agctcatcta
      181 caagcatgcc atttcgacga tcatgccggc gcagccgatc gacatggccg agatcgaacg
      241 cgccttcgcc gcgcaggcgg acaagaaaaa gccggccctg ctccaggagg tgttcctgag
      301 cgcggtgcgg cggtccggcg agccggtgac gatgttcctg gtgaacggcg tcatgctcca
      361 gggcgagatc gccgccttcg acctgttctg catgctgctc cagcgcgagg gcctttcgca
      421 gctcgtctac aaacatgcga tctcgaccgt gcaacccgag cacccgataa gctttataga
      481 ggcggaccaa gaggagtagc cgcctgagcg ttttcgatag aagcagcgag gccgacgtca
      541 gccgcggcgc ccgcgccgtc gtcgccctcc ccgaaatggg gggcgcggcg cggcgcgacc
      601 cggccgcacg cctggacgag gcggcggggc tggcggaggc gatcggcgtc gtcgtcgccg
      661 aaaagttgtc gttcaaggtg cgtgcgccca aggccgccac cctgttcggg tcgggtcagg
      721 tcgagacgat cgccgcggcg gccgagcagg ccgaggccga gctcatcatc atcgacgcgc
      781 cgatcacgcc gatccagcag aagaatctcg aaagcgcgac caaggcgaag gtgatcgacc
      841 ggaccgggct gatcctcgag attttcggcg agcgcgccgc gaccgccgaa gggcggctgc
      901 aggtcgagct cgcgcacctc gattatcagg cggggcggct ggtgcggtcc tggacccatc
      961 tcgagcggca gcgcggcggc ttcggcttcc tgggcggccc cggcgaaacc cagatcgagg
     1021 ccgatcgtcg cctgatccgc gaccgtatgg ccaagcttcg caaggagttg gagcaggtca
     1081 ctcggacgcg gtcgctgcac cgcgcgcgcc ggcaacgggc gccgtggccg gtgatcgcgc
     1141 tggtcggcta taccaatgcc ggcaaatcga ccctgttcaa ccgcctcgcc aatgccgacg
     1201 tcatggcgga aaatctgctg ttcgcgacgc tcgacccgac catgcgcgag atcgcggtgc
     1261 cgggcttcga caaggcgatc ctgtcggata cggtcggctt catctcggac ctgccgaccc
     1321 agctcgtcgc cgccttccgg gcgacgctcg aggaagtgat ctcggccgat ctcatcctcc
     1381 acgtccgcga catctcgcac cccgacagcg acgcccagcg gctcgacgtg gagcaggtgc
     1441 tgaccgagat cggggcgatc ggggaggggg cgtcgccgct gatcgaagcc tggaacaaga
     1501 tcgacctgct cggagccgag gaggcggcga gcgtcggcgc cgaagcggcc cggcgcgagg
     1561 atgtggtgcc gatctccgcg ctgacgggcg agcgggtgga cgcgctgatc gccgccgcgg
     1621 ccgagcggct gcgcgagggg agccgcgtcc gcaaggtgac cttgccggcg ggcgcgggcg
     1681 aggcgatcgc ctggcttcac gccaatggcg aggtgatcga gcagcgcagc gaaggacttg
     1741 agacggaatt tgaagtccgg atctcggatg ccgactgggc ccggttccag acccgccacc
     1801 tcgcctaaag ccgccgcttc gcctcctgcc agagcgcctc gagcgcgtcg atgtccatct
     1861 cggcgagcgg gcgctccgct agggcctcga ccttcctgaa gcgcgcttcg aaccgggccg
     1921 cggaaccgcg caaagccgct tcgggatcga cgccgagatg gcgggcgtaa ttgaccaccg
     1981 agaagaggag gtcgccaagc tcggcggcgc gctcctcgtc gccggcggcg tggtcgagct
     2041 cggccagctc ctcgtcgatc ttggcgcgcg ggccgctcgg gtcgggccag tcgaaaccga
     2101 cgcgggcggc gcgggtctgg agcttctcgg cgcgcttcag cgccggcaga gcgagcgcga
     2161 catgggcgag ggcgcttgga tcctcgcggg cgccgcgctc ggcggccttg agcgcttccc
     2221 agccggggct ttcgccggca gcggcgaaaa tgtgcgggtg gcggcggatc atcttggcgc
     2281 agatcgcggc gatcacgtcg ccgagcgcga aatgaccgag ctcctcggcg atgcgggcat
     2341 ggtagacgat ctggagctgg aggtcgccga gctcgtcctt gagatcggcc atgtcgccgc
     2401 gctcgatggc gtcggcgact tcataggctt cctcgaccgt gtaaggcgcg atggtggcga
     2461 aggtctggac ccggtcccaa gcgcagccgg tgtcgggatc gcgcagccgc gccatgatcg
     2521 cgaccagctc cgcgagagcg gatgcggggg cggctgttgg ggcggatgtt ggcgaaagtg
     2581 cctcggagcg tgatgttggg ggagctgcca caaagcctgt cctcaaagtg tgataatatg
     2641 cattatgtaa aatgacgtca tggcttcagg gcgggctagg ccccacctcg caggcggaca
     2701 gccacgacta cacattccgt tccccgcgca ggccgggggt ccagctgcgc tggagtctgg
     2761 gccccggcct tcgccg
//
LOCUS       DASVRK010000154         6503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_215654, whole genome shotgun sequence.
ACCESSION   DASVRK010000154 DASVRK010000000
VERSION     DASVRK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215654"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VF589_06025"
     CDS             complement(<1..1209)
                     /locus_tag="VF589_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HEX8257170.1"
                     /translation="MSLVVDLSALGSAGFIIQGDAPGDQAGRAVASAGDVNGDGIADI
                     IVGAPRADQGAALDFGAAYVIFGKAGGFSGTLDLTGLAPADGFAIRGSAEYDRAGFSV
                     ASAGDVNGDGFDDVIVGAYANDGGGTSAGAAYVVFGRASGLGTIDLAALAPARGFMIR
                     GDAANDYAGSSVSSAGDFNGDGIDDLIVGAPGGDDGGDKAGEAHVIFGKTRGLGTIDL
                     GALAPSDGFIIQGDLDGDRAGVSVASAGDVNGDGLGDIIVGAEGGDDGGDLAGEAYIL
                     FGRTAGSGTIDLTGLSAADGFIIRGAAPADFAGFSVASAGDVDGDGYADLIVGAPGGG
                     ASGKAYVLFGKAGGFGTIDLAALTADDGFAIEGDAIDDEAGISVSSAGDVNGDGFDDV
                     MIGARGGDDGG"
     gene            complement(1262..3703)
                     /locus_tag="VF589_06030"
     CDS             complement(1262..3703)
                     /locus_tag="VF589_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257171.1"
                     /translation="MATIHLSALGTGGFVIQGDGISDYAGFSVSSAGDVDGDGRDDLL
                     IGAHANDAGGINAGAAYLIFGKAAESGTIDLAALAASDGFKIQGEAANDYAGRSVASA
                     GDVNGDGFADLLIGAPYNDRGGYNSGAAYVIFGKASGFGPIDLATLTAAQGIRILGDA
                     EADFAGRDVASAGDVNNDGFGDLIVGADRNDGGGTDAGAAFILFGKARKPGTIDLSNL
                     AATDGFKIQGDAPADVAGGSVSSAGDVNGDGFGDLLIGAQGSGSGPVETGAAYVLFGK
                     ARGFGPVDLAALAPADGFRIQGEADLDSAGRSVSAAGDVNGDGFGDILVGAQGNDSGG
                     PYAGAAYVIFGKASGFGPVDLAALAADDGFKIQGAAAANYAGRGLSSAGDVNGDGYDD
                     ILVGADRNAGGGVSAGAAYVIFGKAGGFGLVDLAALPAADGFKVQGSTGDYAGFSVSA
                     GDANGDGFSDLLIGAHGNDGGGFNAGAAFVLFGAAPTASVTRIGSTGGQTIRGGIGDD
                     LLDGRDGDDNLFGGEGDDQLLGGAGRDALHGGGGADAMAGGSGNDILNGGTGGDAMNG
                     GSGHDAYHVDNAGDRISEAEGNGSDRLFARTSYVLAAEASVEVMSTNANAGTSAIHLT
                     GNAGNNRISGNNGGNILQGRDGADSLSGLGGADRLWGGGGRDVLAGGAGADRFVFDSA
                     PSATLNFDRIADYSVADDTIMLDRRIYAGIADGTLAAAAFRAGTAAADASDRIIHHSA
                     TGNIFYDSDGAGGAAQILFAKVGAGLALTRLDFFAFTGADPAAAARRGMAPTAEREAV
                     ADFTPMQAIGWAEIP"
     gene            complement(3862..5388)
                     /locus_tag="VF589_06035"
     CDS             complement(3862..5388)
                     /locus_tag="VF589_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8257172.1"
                     /translation="MAGVIDLAHAAPFRLGKVEVHPGTRQLIRDGRSETLEPRVMKVL
                     VALARAEGAIVTRDELTQSCWGGRVVGENAIHRAISRVRDVALKFAGDSFQLETITKV
                     GYRLIVPGAEPPASAALAAEPVAPAIDRRRLLLGGAAALGLAGAAAWFGNRPPAGGGP
                     PAEALELYRQGQLLQRQSLDEQVKQAVAFFSQAVKIDPSYADAWGALALSYRNILESY
                     ADGQEASAANLARSAARRALALDPGNADAQAALILIEPHFRRWGAFEKDVRGLLAEHP
                     NHWVLRAEAAHILYQVGRWEEGIEHGRKVLEIDPFLPMAHHSLARALWSAGQLQEANS
                     LIDKAVERWPAHYLLWNTKYNILAFSGRAAAAIAAFSDPERPTFGVAPTAAYNRAVLA
                     RAIETGSPGDVAAALAIHRSQALAQVSNVPHSALVFAALGQADLTFEALDAYFFGKGP
                     MAGAAPAAIGPYTRRYTSFLFTPPMAPLRSDPRFTALTAAIGLDDYWRATNSAPDYRR
                     "
     gene            5796..>6503
                     /locus_tag="VF589_06040"
     CDS             5796..>6503
                     /locus_tag="VF589_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257173.1"
                     /translation="MADFYQRMYRGTEANEIIHGSALNDDIHGIAGHDQLYGLGGDDA
                     LNAGEHGDTMWGGDGDDFLGGSLEVDHMYGEAGADRLFGLLGGDFMYGGAGTDFLVGE
                     AGADYMSGGDQADKLHGGGDDDNMLGGRGHDRMGGDAGNDHMDGGSGDDSLSGGSGHD
                     HLDGGAGDDQMEGEEGNDWLDGGVGNDGLNGGIGNDTLFGGEGADTIMAEDGNDFLDG
                     GEHNDRLYGDGGDDILTG"
BASE COUNT         1001 a         2238 c         2300 g          964 t
ORIGIN      
        1 gccgccatcg tcgccgccgc gcgcgccgat catgacgtcg tcgaagccgt cgccgttgac
       61 gtccccggcc gatgacacgc tgatccccgc ctcgtcgtcg atcgcgtcgc cctcgatggc
      121 gaagccgtcg tcggcggtga gcgcggcgag gtcgatcgtg ccgaagccgc cggccttgcc
      181 gaacaggaca taggccttgc ccgaggcgcc gccgcccggc gcgccgacga tcaggtcggc
      241 atagccgtcg ccgtcgacat cgcccgccga agcgacgctg aagccggcaa agtcggcggg
      301 cgcggcgccc cggatgatga agccgtccgc cgcggacagg ccggtgagat cgatggtccc
      361 ggagccggcc gtcctgccga acagtatata ggcctcgccg gcgagatcgc cgccatcgtc
      421 gccgccttcc gccccgacga tgatgtcacc gaggccgtcg ccgttgacgt cgccggcgga
      481 cgccacgctg acgcccgcgc ggtcgccgtc caggtcaccc tggatgatga agccgtcgga
      541 gggggcgagc gcgccgagat cgatcgtccc gagcccgcgc gttttgccga agatgacatg
      601 ggcctccccg gctttgtcgc cgccatcgtc gccgccgggt gcgccgacga tcaggtcgtc
      661 gatgccgtcg ccgttgaaat cgcctgccga ggaaacgctc gagcctgcat agtcgttggc
      721 ggcgtcgccg cggatcatga agccgcgcgc cggggccaaa gcggcaagat cgatcgtgcc
      781 gagcccgctc gccctgccga agacgacata ggccgcgccg gcactggttc cgccgccgtc
      841 gttcgcatag gcgccgacga tcacgtcgtc gaagccgtcg ccgttgacgt cgccggcgga
      901 ggcgacgctg aacccggcgc gatcatattc ggcgctgccc cggatggcga acccgtcggc
      961 aggcgccagt ccggtgaggt cgagcgtgcc gctgaacccg cccgccttgc cgaagattac
     1021 gtaggcggcg ccgaagtcca gggcggcgcc ctggtccgcg cgcggggcgc cgacgatgat
     1081 gtcggcgatg ccgtcgccat tgacgtctcc cgccgacgcg acggcgcgcc ccgcctggtc
     1141 gccgggggca tcgccctgga tgatgaagcc ggcggagccg agcgcggaga ggtcgacgac
     1201 aaggctcaca acggatctcc tgggtagccg gggccggggg gcgccgtctt caagacgccg
     1261 ctcacggtat ctccgcccag ccgatcgcct gcatcggcgt gaaatccgcg accgcttcgc
     1321 gctcggccgt cggcgccatg ccgcgacgcg ccgcggcagc ggggtcggcc ccagtgaagg
     1381 cgaagaagtc gagccgcgtc agcgccagcc ctgcgccgac tttcgcgaac aggatctgcg
     1441 ccgcgccgcc cgccccgtcg gaatcgtaga agatgttgcc ggtcgcggaa tggtggatga
     1501 tccggtccga tgcgtccgcg gcggccgtcc cggcgcggaa ggcggcggcc gccagcgtcc
     1561 cgtcggcgat gccggcatag atgcggcgat cgagcatgat ggtgtcgtcg gccacggaat
     1621 aatcggcgat ccggtcgaag tttagcgtgg cgctcggcgc gctgtcgaag acgaagcggt
     1681 ccgcgcccgc cccgccggcc agcacgtccc tgccgccgcc gccccacagc cggtcggcgc
     1741 cgcccaggcc gctcaagctg tcggccccgt cgcgcccttg caggatgttg ccgccattgt
     1801 tgcccgagat ccggttgttc ccggcattgc cggtgaggtg gatcgccgag gttccggcat
     1861 tggcgttcgt gctcatcacc tcgaccgagg cttcggcggc cagcacatag ctggtgcgcg
     1921 cgaacaggcg gtcggaaccg ttgccctccg cctcggaaat gcggtcgccg gcattgtcga
     1981 catgataggc atcgtggccc gatccgccgt tcatggcatc gccgccggtg ccgccgttca
     2041 gaatgtcgtt gcccgatccg ccggccatcg cgtccgcgcc gccgccgccg tggagcgcgt
     2101 cgcggccggc gccgccaagc aattggtcgt cgccctcgcc gccgaacaga ttatcgtcgc
     2161 cgtcgcgccc gtcgagcaaa tcgtcgccga tgccgccgcg gatcgtctgg ccgccggtcg
     2221 agccgatccg ggtcacgctc gcggtcggcg cggcgccgaa caggacgaag gccgcgccgg
     2281 cattgaagcc gccgccgtca ttgccgtggg cgccgatgag caggtcggag aagccgtcgc
     2341 cattggcgtc gcccgccgaa acgctgaagc cggcataatc gcccgtgctg ccctggacct
     2401 tgaacccgtc cgcggcgggg agggcggcga gatcgaccag gccgaagccg cccgccttgc
     2461 cgaagattac ataggcggcg ccggcggaga cgccgccgcc ggcgttgcgg tccgcgccca
     2521 cgaggatgtc gtcataacca tcgccattga cgtcgcccgc cgaggagagg ccccggcccg
     2581 cataattggc ggccgcggcg ccctggatct tgaagccgtc gtcggccgcc agtgcggcga
     2641 gatcgaccgg gccgaacccg ctcgctttgc cgaagatgac ataggccgcg ccggcatagg
     2701 ggccgccgct gtcattgcct tgggccccga cgaggatatc gccgaagccg tcgccgttga
     2761 cgtcgcccgc cgcggacacg ctgcggccgg cggaatcgag atcggcctcg ccctggatcc
     2821 tgaaaccgtc ggcgggggcg agcgcggcga gatcgaccgg accgaagccg cgcgccttgc
     2881 cgaaaaggac gtaggcggcg ccggtctcga ccgggccgct gccgctgccc tgggcgccga
     2941 tcagcaggtc gccaaagccg tcgccattga cgtcgccggc cgaggagacg ctgccgccgg
     3001 cgacgtcggc gggcgcgtcg ccctggatct tgaagccgtc ggtggcggcg aggttcgaca
     3061 ggtcgatcgt gccgggcttt ctcgccttgc cgaacaggat aaaggccgca ccggcatcgg
     3121 tgccgccgcc gtcgttgcgg tcggcgccga cgatgaggtc gccgaagccg tcattgttga
     3181 cgtcgcccgc cgaagcgacg tcccggccgg cgaagtccgc ttcggcatcg cccaggatcc
     3241 tgatcccctg ggcggcggtg agggtggcga gatcgatcgg gccgaacccg ctcgccttgc
     3301 cgaagatcac gtaggccgcg ccggaattat agccgcctcg gtcgttgtaa ggcgcgccga
     3361 tgaggaggtc ggcaaagccg tcgccgttga catctcccgc cgaggcgacg ctccggccgg
     3421 cataatcgtt ggcggcttcg ccctggatct tgaagccgtc ggaggcggcg agggcggcaa
     3481 ggtcgatcgt cccggactcg gccgccttgc cgaatatcag ataggccgcg ccggcattga
     3541 tcccgcccgc gtcattcgcg tgggcgccga tcaggaggtc gtcgcggccg tcgccatcga
     3601 cgtcgccggc cgaggagacg ctgaagccgg cataatcgct aatgccgtcg ccttggatca
     3661 cgaagccgcc cgtcccgagc gccgacaagt ggatggtcgc cacgatagag tcccccctca
     3721 gccgccatga gtgtccaccg taagcggcct gggcgaaagg atacggccct gatggaagcc
     3781 tgacggaagc ctgatatatt cggcccgcgt catagattct gttttgtttt ccgcaatttt
     3841 ggccctggcg gaccgacgga gctagcggcg gtagtccggg gcggaattcg tcgcgcgcca
     3901 ataatcgtcc aggccgatcg ccgccgtcag cgcggtgaag cgggggtcgg agcggagcgg
     3961 cgccatcggg ggcgtgaaca ggaatgaggt gtagcggcgc gtatagggcc cgatcgccgc
     4021 gggtgccgcg ccggccatcg gccccttgcc gaagaaatag gcgtcgagcg cctcgaacgt
     4081 caggtcggcc tgccccagcg ccgcgaacac cagggccgag tgggggacgt tcgagacctg
     4141 cgcaagcgcc tgcgaccggt ggatcgcgag ggcggccgcg acgtcgcccg ggctgccggt
     4201 ctcgatggcg cgcgccagca cggcgcgatt gtaggcggcg gtaggggcga cgccgaaggt
     4261 cggccgctcg ggatcggaga aggcggcgat cgccgccgcc gcccgcccgc tgaaggcgag
     4321 gatgttatat ttggtgttcc acaagaggta atgggccggc cagcgctcga cggccttgtc
     4381 gatcaggctg ttggcttcct gcaactggcc cgcgctccac agcgcccgcg cgagcgaatg
     4441 gtgcgccatc ggcaggaatg gatcgatctc gagcaccttg cggccatgct cgatcccttc
     4501 ctcccagcgg ccgacttgat agaggatgtg ggcggcctcc gcgcgcagca cccaatggtt
     4561 gggatgctcg gccagcaagc cccgaacatc cttctcgaac gccccccaac ggcgaaaatg
     4621 cggctcgatc aggatcagcg cggcctgcgc gtcggcattg cccggatcca gcgcgagcgc
     4681 gcgccgggcc gccgaacgcg ccaggttcgc cgcgctggcc tcctgcccgt cggcatagct
     4741 ttccaggatg ttgcggtagg aaagcgccag cgcgccccag gcgtcggcat aggaagggtc
     4801 gatcttcacc gcctggctga aaaaggcgac ggcctgcttg acctgctcgt ccaggctctg
     4861 ccgctgcagc agctggcctt ggcgatagag ctccagcgct tcggctggtg ggccgccgcc
     4921 ggccggcggc cggttcccga accaggctgc cgctcccgcg agccccagcg ccgcggcgcc
     4981 gccgagcagg aggcggcgcc ggtcgattgc cggtgcgacg ggttcggcgg caagggcggc
     5041 gctcgcgggc ggttcggcgc cgggaacgat caagcggtag ccgaccttgg tgatcgtctc
     5101 gagctggaac gagtctcccg cgaatttgag ggcgacgtcg cgaacgcggg agatggcgcg
     5161 gtggatcgcg ttctcgccga ccacccggcc gccccagcag gattgggtca gctcgtcgcg
     5221 cgtcactatg gcgccctcgg cccgcgcgag cgcgaccagc accttcatca cccggggctc
     5281 gagcgtttcg ctccgcccgt cgcggatgag ctgccgcgtt ccggggtgca cctcgacctt
     5341 ccccagccgg aacggcgccg catgggcgag atcgatcacc cctgccatgt ctccgctctg
     5401 gcttgccacg aatcatctcc gggcctcggc ggccgatccg ccgagatcgg tgcatggtgg
     5461 cacggattgc gagcgaccgg cagccccgcc tcgtcggcgc ctctgagtgc cgggatcact
     5521 gagtgagatc gaattaatcg acttcttcga tcaccggaag aagtttagct tcttcaatgg
     5581 actagggtgg ggcccaacgc gaagacggcg gcgcagggag ctgcgctcac acgggcgaca
     5641 cgcgctccac gaaatggtct ccaataggcc atgcggccgg aataaagccc tgccgcctcc
     5701 gttctggatc tgactgccgg ccggtcctcc gagcctggac agcccgatgg ctgattggtt
     5761 caaccacatg cccgttcgcg ggggggagta gtgaaatggc tgatttctat caacgcatgt
     5821 atcgcggcac cgaggccaat gagatcatcc acggcagcgc gctcaacgac gacattcacg
     5881 gcattgccgg ccacgatcag ctttacgggc tcggcggcga cgacgcgctc aacgccggcg
     5941 agcatggcga tacgatgtgg ggcggcgacg gcgacgactt cctgggcggc agcctcgagg
     6001 tcgatcacat gtatggcgag gccggcgccg accggctgtt cggcctgctc ggcggcgact
     6061 tcatgtatgg cggcgccggc accgacttcc tggtcggcga ggcgggcgcc gattatatga
     6121 gcggcggcga ccaggccgac aagcttcatg gcggcggcga cgacgacaat atgctcggcg
     6181 ggcgcggcca cgaccggatg ggcggcgatg ccggcaacga ccatatggac ggcggctccg
     6241 gcgacgacag cctcagcggc ggttccggcc acgaccatct cgacggcggc gccggcgacg
     6301 accagatgga aggcgaagaa gggaatgact ggctcgacgg cggtgtgggc aacgacggcc
     6361 ttaacggggg gatcggaaac gacactctct tcgggggtga gggcgccgat acgatcatgg
     6421 ccgaagacgg gaacgacttc ctcgacggcg gcgagcataa cgaccgcctc tatggcgatg
     6481 gcggcgacga catcctgacg ggt
//
LOCUS       DASVRK010000155         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_28886, whole genome shotgun sequence.
ACCESSION   DASVRK010000155 DASVRK010000000
VERSION     DASVRK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28886"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF589_06045"
     CDS             <1..399
                     /locus_tag="VF589_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8257174.1"
                     /translation="AFQPEVFDVGVNIFGVTNWVRTLKSIPPYWASFRESLYDEMGDP
                     ATDETRHRAISPLFHANNIVKPMLVVQGANDPRVLQVESDEIVAAVKANGVPVEYLVF
                     PDEGHGFLRKQNRIDASEAYLRFLDTHLRR"
     gene            474..1415
                     /locus_tag="VF589_06050"
     CDS             474..1415
                     /locus_tag="VF589_06050"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX8257175.1"
                     /translation="MMPPFPHRHLLGIDGLSAADVRMILDEAERWAAFNRGVRRQDQR
                     LAGLTQVNAFFENSTRTSLSFEMAGKRLGAQVVNFQAAQSSVKKGESLIDTALTINAM
                     RPDLFVIRHEAPGAARDVADVIDCAVINAGDGHGEHPTQALLDALTIRRRKGRIEGMT
                     VAICGDIRHSRVARSNMILLPLLGARVRLVGPESLLPERGELGGAAAFTDMGEGIDGA
                     DVVMMLRIQRERLPAGLAETLGDYRARYGLTRERLEAAAPDATVMHPGPMNRGVEIDG
                     DVADDPARSAIRDQVELGVAVRMACLDLLTRERRGDK"
     gene            complement(1469..>2502)
                     /locus_tag="VF589_06055"
     CDS             complement(1469..>2502)
                     /locus_tag="VF589_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8257176.1"
                     /translation="GNHRASLSIALAQIALGKAGEATAQLEELARTAAPADVGLAFAL
                     AGQPQRAIEMLEPAARSPEANGRIRQNLALAYALAGDWQKARVTAAQDVSPAQLGDRL
                     QQWAAFAQPKASWDQVAGLLGVTPAEDPGQPVHLALAPPSPAAGQYAAVEYPQPVEAA
                     PAAPISFAEAADPNQPIESAPQPVRTEEIRYAEAARSLVDAPVIRASAEIGDIADVEL
                     PAFVPVKKPRIAGRSERAFEERVARRHVGRFVVQIGAYGSSAAVERAWAESSKRYPFS
                     DAQEPLSTTVTLPGKGTFHRLSVSGFGTHLAASRLCSSIRAKGGACFVRTTAGDAPVQ
                     WASRYTGRA"
BASE COUNT          385 a          823 c          940 g          354 t
ORIGIN      
        1 gccttccagc cggaggtgtt cgacgtcggt gtcaacatct tcggcgtgac caactgggtg
       61 cggacgctga agtcgatccc gccttactgg gcctcgttcc gcgaatcgct ctatgacgag
      121 atgggcgacc cggccaccga cgagacccgg caccgggcga tctcgccgct gttccacgcc
      181 aataatatcg tgaagccgat gctggtcgtg cagggcgcga acgatccgcg cgtgctccag
      241 gtcgagagcg acgagatcgt cgcggcggtc aaggcgaacg gggtgccggt cgaatatctg
      301 gtgttcccgg acgagggcca cggtttcctc cggaagcaga accggatcga cgcgtcggag
      361 gcctatctga ggttcctcga cacgcatctg aggcggtgag tgcttcctcc ccgcggggaa
      421 aggcgatgac ggggcgaggg attgaggcgg ccgcgcgcca aggctaagcc gcgctgatgc
      481 cccccttccc tcaccgccac ctgctcggca tcgacggcct gtcggccgcc gacgtccgga
      541 tgatcctcga cgaggcggag cggtgggccg ccttcaaccg cggtgtacgc aggcaggacc
      601 aaaggttggc cggcctcacc caggtcaacg ccttcttcga gaacagcacc cggacctcgc
      661 tgtcgttcga gatggccggc aaacggctcg gcgcgcaggt ggtaaatttt caggccgcgc
      721 aatcgagcgt gaagaagggc gagagcctga tcgacaccgc gctcaccatc aacgcgatgc
      781 gccccgacct gttcgtgatc cggcacgagg ctcccggcgc ggcgcgcgac gtggcggacg
      841 tgatcgactg cgcggtgatc aatgccggcg acggccatgg cgagcatccg acgcaggcgc
      901 tgctcgatgc gctcaccatc cgccgccgca aggggcgcat cgaagggatg acggtcgcga
      961 tctgcggcga catccgccac agccgggtgg cgcggtcgaa catgatcctg ctgcccctgc
     1021 tcggcgcaag ggtgaggctg gtgggaccag aaagcctgct gccggagcgg ggtgagctcg
     1081 gcggagcggc ggccttcacc gacatgggcg aagggataga cggcgccgac gtggtgatga
     1141 tgctgcggat ccagcgcgag cggctgccgg cgggcctggc cgagacgctc ggcgattacc
     1201 gcgcccgcta cgggctgacc cgcgagcggc tcgaggcggc cgcgccggac gcgaccgtga
     1261 tgcatccggg gccgatgaac cgcggggtgg agattgacgg cgacgtcgcc gacgatcccg
     1321 cgcgctcggc gatccgcgat caagtcgagc tcggagtcgc ggtcaggatg gcctgcctcg
     1381 acctgctcac gcgggagcgc cgcggcgata aataaaaggg cggcgatcct tcgaccgccg
     1441 ccctcgaatc aaatcagctc agctcaggtc aggcgcggcc ggtatagcgc gaggcccatt
     1501 gcaccggcgc gtcgccagcg gtggtgcgca cgaagcaggc gccgcccttg gcgcggatcg
     1561 agctgcacag gcgcgaggcc gccagatggg tgccgaagcc ggataccgac agccggtgga
     1621 acgtgccctt gccgggcaaa gtaacggtgg tgctgagcgg ctcctgggcg tcgctgaacg
     1681 ggtagcgctt gctcgattcg gcccaggcgc gctcgaccgc ggcggatgag ccgtaggcgc
     1741 cgatctggac cacgaagcgg ccgacgtggc ggcgggcgac ccgctcctcg aaggcgcgct
     1801 cgctgcggcc ggcgatgcgc ggcttcttga cgggcacgaa ggcgggaagc tcgacgtcgg
     1861 cgatgtcgcc gatctcggcg gacgcgcgga tcaccggcgc gtcgaccagg ctccgggccg
     1921 cctcggcgta gcggatttcc tcggtccgga ccggctgcgg cgccgattcg atcggctggt
     1981 tcgggtcggc ggcctcggcg aaggagatcg gcgcggcggg cgccgcttcg accggctgcg
     2041 gatactcgac ggcggcgtac tggccggcgg cggggctcgg cggagccaga gcgagatgaa
     2101 ccggctggcc cggatcctcg gcgggcgtga cgccgagcag gccggcgacc tggtcccagc
     2161 tcgccttggg ctgcgcgaac gccgcccatt gctggagccg atcgccgagc tgcgccgggg
     2221 agacgtcctg cgccgcggtg acgcgggcct tctgccagtc gccggccagc gcgtaggcga
     2281 gcgccagatt ctggcggatg cggccattgg cctcgggaga gcgggcggcg ggctcgagca
     2341 tctcgatcgc ccgctgcggc tgtccggcaa gcgcgaaagc caggccgaca tcggcgggag
     2401 cggcggtgcg cgccagctct tcgagctggg cggtggcctc gcccgctttg ccgagcgcga
     2461 tctgggcgag cgcgatgctg agcgaggcac gatgattgcc gg
//
LOCUS       DASVRK010000156         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_278575, whole genome shotgun sequence.
ACCESSION   DASVRK010000156 DASVRK010000000
VERSION     DASVRK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278575"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VF589_06060"
     CDS             complement(<1..362)
                     /locus_tag="VF589_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX8257177.1"
                     /translation="MATLKHLLPPLLAALSACTMERPASPGEGPATETYRARGQEPGW
                     ALTIAEGMIDYRGNYGEKRIAVPRPDPRTTFNGHRYETPRLTVDVTHGRCNDAMSGHG
                     YADQVVVIADGETYRGCGG"
     gene            complement(350..1147)
                     /locus_tag="VF589_06065"
     CDS             complement(350..1147)
                     /locus_tag="VF589_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8257178.1"
                     /translation="MPREITAFSNPLVKEVRALREKKGRRAQGMFMAEGLRILTEARE
                     AGRLPAILFYAGGTRHPLLDALVAEVEAAHGDVIETNADILHKLSGKDNPQTVIGVYR
                     GFEMALERIDREAAPLWIVAQALRDPGNLGTILRTGDAVGAGGLILVDDCVDPFSVEA
                     VRASMGALFTQQIAAAGWADFVRWLRSGAGQLIGTSLKAEQDYQAPHYAAPVFILVGN
                     EAQGLPESYEAECDVLVKIPMLGKADSLNAAVATAVMAYEVINQWRR"
     gene            complement(1272..1544)
                     /locus_tag="VF589_06070"
     CDS             complement(1272..1544)
                     /locus_tag="VF589_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX8257179.1"
                     /translation="MTVVSRTVAVLNKRGLHARASAKFVTLASGLAVPVEVEKDGGRV
                     CGTSIMGLMMLGAAMGDTITISAEGEGADAAVAALAELVEAKFGEE"
     gene            complement(1541..1948)
                     /locus_tag="VF589_06075"
     CDS             complement(1541..1948)
                     /locus_tag="VF589_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX8257180.1"
                     /translation="MIGLVLVTHGRLAAEFVVAMEHVVGPQQRIEAICIGPEDDMEAR
                     RNDIAEAIAKVDEGAGVIILTDLFGGTPSNLAISLMKSEAVEVIAGVNLPMLIRLEGA
                     RKSMDVKAAVAAAREAGRKYISVASEILGEAAA"
     gene            complement(1958..2902)
                     /gene="rapZ"
                     /locus_tag="VF589_06080"
     CDS             complement(1958..2902)
                     /gene="rapZ"
                     /locus_tag="VF589_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX8257181.1"
                     /translation="MSAGSTPERILLVTGLSGAGKSTVLRTLEDIGWEVVDNLPLSLL
                     EHLLSTPLPEGVTRRRRPLAIGVDSRTRDFDAQTIVAQVKALGDERRLPIGTLFLDCT
                     GSELIRRYSETRRRHPLALDRPASDGISAERELMAPLKRWSDYVIDTTDTEANQLQQQ
                     VRQLFGGGEQAPNLHIMSFGFARGLPRSADTVFDLRFLRNPHWVDELRDLTGLDAAVG
                     DYIRTDPAYEDALERMEALLLALLPRYGAEGKSYFSVAFGCTGGRHRSVHVAERVAAR
                     LREAGFSPTVRHRDLASRPRDPVEGDPAGGARTAQTED"
     gene            complement(2899..3342)
                     /locus_tag="VF589_06085"
     CDS             complement(2899..3342)
                     /locus_tag="VF589_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX8257182.1"
                     /translation="MTVSAISSETLHASCVAIGGRAVLISGGSGSGKSDLALRLIDRG
                     AELVSDDYTIVRRVEGQLIASPPQTIAGKLEVRGLGILERAYVQGAPVCLAIDLARGA
                     ARMPEPDEALLVAGIRLPAIALNALEASAPIKAELALERYGLRPA"
     gene            complement(3339..>4005)
                     /locus_tag="VF589_06090"
     CDS             complement(3339..>4005)
                     /locus_tag="VF589_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8257183.1"
                     /translation="DVTHELKNPLASLRSAVDSLERVDDPALRGQLLDVVRHDVVRLD
                     RLVVDIAEVSRLDAELSRARFEPVDLGRTIEALLEGWDARAAERGVRIAFARPRAGTA
                     VAMGEEARLVRVFDNIVDNAISFSPEHGLVEIAAAQTDGQVTVSVEDEGPGLPPGPRE
                     AIFNRFHSIRPDGEAFGRHSGLGLAIAKAIVEGHNGRIEAEDRHDGRSGARFVIRFPG
                     AGG"
BASE COUNT          612 a         1476 c         1320 g          597 t
ORIGIN      
        1 ccgccgcagc cgcgataggt ctcgccgtcc gcgatcacca ccacctggtc ggcatagccg
       61 tggccgctca tcgcatcgtt gcagcggccg tgggtcacgt ccaccgtcag gcgcggcgtc
      121 tcgtagcggt ggccgttgaa ggtcgtgcgg gggtcgggcc gcggcacggc gatccgcttc
      181 tcaccataat tgccgcgata gtcgatcatc ccctcggcga tggtcaacgc ccagccgggc
      241 tcctgtccgc gcgcgcggta ggtctccgtc gccggcccct ctccgggcga ggcgggacgc
      301 tccatcgtgc aggcggagag cgcggccagc agcggcggca ggagatgctt cagcgtcgcc
      361 attggttgat cacctcatag gccatcaccg ccgtcgccac cgcggcgttg aggctgtcgg
      421 ccttgcccag catcgggatc ttcaccagca cgtcgcactc cgcttcgtag ctttccggca
      481 gcccctgcgc ctcgttgccg accaggatga agaccggcgc ggcgtaatgc ggcgcctgat
      541 aatcttgttc ggccttcagg ctggtcccga tcagctgacc ggcccctgaa cgcagccagc
      601 gcacgaagtc ggcccagccc gccgccgcga tctgctgggt gaacaaagct cccatcgacg
      661 cccgcaccgc ctcgaccgag aacggatcga cgcaatcgtc gaccaggatc agcccgcccg
      721 cgccgaccgc gtcgccggtg cgaaggatgg tgccgagatt gcccgggtcc cgcagcgcct
      781 gcgccacgat ccagagcggc gccgcctccc ggtcgatccg ctccagcgcc atctcgaagc
      841 cgcgatagac gccgatcacc gtctgcggat tgtccttgcc ggaaagcttg tggaggatgt
      901 cggcattggt ctcgatcacg tcgccatgcg ccgcctcgac ctccgccacc agcgcgtcca
      961 gcagggggtg gcgcgtgccg cccgcataga agaggatcgc cggcagccgc cccgcctcgc
     1021 gcgcctcggt caggatgcga agcccctcgg ccatgaacat gccctgcgcg cggcggccct
     1081 tcttctcccg aagcgcccgc acttccttga cgagcgggtt cgaaaaagcg gtgatctcgc
     1141 gcggcattac gcgttccccg gcgaaggccg gggcccagac tcgagcgcag ctggacccct
     1201 gccttcgcag gggaacagtg ccctcgctgg accccagccc accccgagga gcacggagcg
     1261 gcccaacccc ctcattcctc gccgaacttg gcctcgacca gctccgccag cgccgccacc
     1321 gccgcgtcgg cgccttcgcc ctcggcgctg atcgtgatcg tgtcgcccat cgcggcgccc
     1381 agcatcatca gccccatgat cgatgtgccg cacaccctgc cgccatcctt ctcgacctcg
     1441 accggcaccg cgaggccgga ggcgagggtc acgaacttgg cgctggcgcg ggcgtggagg
     1501 ccgcgcttgt tcagcaccgc gacggtccgg ctcaccacgg tcaggccgcc gcctcgccca
     1561 aaatctcgga cgcgaccgag atatatttgc gccccgcctc gcgcgccgcg gccaccgccg
     1621 ccttgacgtc catcgacttg cgcgcgcctt cgagccggat cagcatcggc aggttgaccc
     1681 cggcgatcac ctcgaccgcc tccgatttca tcagcgagat ggcgaggttc gacggcgtgc
     1741 cgccgaacag gtccgtaagg atgatcacgc cggcgccctc gtcgaccttg gcgatcgcct
     1801 cggcgatgtc gttgcggcgc gcttccatat cgtcctcggg gccgatgcag atcgcctcga
     1861 tccgctgctg cggaccgacc acatgctcca tggccaccac gaattcggcc gcgagccgcc
     1921 catgcgtaac cagcaccaaa ccgatcattc agaacaccta atcttccgtc tgcgcggtcc
     1981 gggcccctcc ggccggatcg ccttcgaccg gatcccgcgg ccgggaggcg agatcgcgat
     2041 gtcgaacggt gggggaaaat cccgcctcgc gcaacctcgc cgccacccgc tcggcgacat
     2101 gtaccgatct atgacggccg cccgtacacc cgaaagcgac cgaaaaatag gattttccct
     2161 ccgccccgta gcgcggcagc agcgcgagca gcagcgcctc catccgctcc agcgcgtcct
     2221 cataagcagg gtcggtgcgg atgtagtcgc cgaccgccgc gtcgagcccg gtcaggtcgc
     2281 gcagttcgtc gacccaatgc gggttcctga ggaagcgcag gtcgaacacc gtgtcggcgc
     2341 tgcgcggcag accccgggcg aagccgaacg acatgatgtg gaggttcggc gcctgctcgc
     2401 cgccgccgaa cagctggcgg acctgctgct ggagctggtt ggcctcggtg tcggtggtgt
     2461 cgatcacata gtccgaccag cgcttcagcg gcgccatcag ctcgcgctcg gcggagatgc
     2521 cgtcgctggc cggccggtcg agcgcgagcg ggtggcggcg gcgggtctcc gaatagcggc
     2581 ggatgagctc ggacccggtg cagtcgagga acaatgtccc gatcggcagc cgccgctcgt
     2641 cgcccagcgc cttgacttgg gcgacgatgg tctgggcgtc gaagtcgcgg gtgcggctgt
     2701 cgacgccgat cgcgagcggc cgccgccggc gcgtgacgcc ttccggcagc ggcgtcgaca
     2761 gcagatgctc gagcagcgac agcggcagat tgtcgaccac ttcccagccg atatcctcga
     2821 gcgtccgaag caccgtcgac ttgccggcgc ccgacaggcc ggtgaccagc aggatccgct
     2881 cgggcgtgga gccggcgctc atgcgggccg cagcccgtag cgctcgagcg ccagctcggc
     2941 cttgatcggc gccgaggctt cgagggcatt gagcgcgatc gccggcagcc taatccccgc
     3001 gacgagcaac gcttcgtccg gctccggcat ccgcgccgcg ccgcgggcga ggtcgatcgc
     3061 caggcagacc ggcgcgccct gaacataagc cctttcgagg atgccgaggc cgcgcacttc
     3121 gagcttgccg gcgatggtct gcggcggcga cgcgatcagc tggccttcga cgcggcgaac
     3181 gatcgtataa tcgtcgctca ccagctccgc gccgcggtcg atcaggcgca gcgcgaggtc
     3241 ggacttgccg ctccccgagc cgcccgagat cagcaccgcc cggccgccga tggcgacgca
     3301 ggaagcgtgc agcgtctccg acgagatcgc cgacaccgtc atccgcccgc gcccgggaag
     3361 cggatcacga agcgcgcgcc cgaacggccg tcatggcggt cctcggcttc gatcctgccg
     3421 ttatggccct cgacgatcgc cttggcgatg gcgaggccga ggccggaatg gcggccgaac
     3481 gcctcgccgt cggggcggat gctgtggaag cggttgaaga tcgcctcgcg cggcccgggc
     3541 ggcagccccg gtccttcatc ctcgacgctg accgtcacct ggccgtcggt ctgcgccgcc
     3601 gcgatttcga cgaggccgtg ttcgggcgag aaggagatgg cgttgtcgac gatattgtcg
     3661 aacacccgca ccagtcgcgc ttcctcgccc atcgcgacgg cggtaccggc ccgcgggcgg
     3721 gcgaaggcga tccgcacccc gcgctcggcc gcccgcgcgt cccagccttc gagcaaagcc
     3781 tcgatcgtgc ggccgagatc gaccggctcg aaccgcgcgc gcgaaagctc ggcgtcgagg
     3841 cgggagactt cggcaatgtc gaccaccagc cggtcgaggc ggacgacgtc atgacggacg
     3901 acatccagca actggccccg aagcgccgga tcgtcgaccc gctcgaggct gtctaccgcc
     3961 gagcgcaagg aggcgagcgg gttcttgagt tcgtgcgtca cgtcg
//
LOCUS       DASVRK010000157         8102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_403431, whole genome shotgun sequence.
ACCESSION   DASVRK010000157 DASVRK010000000
VERSION     DASVRK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403431"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..523
                     /locus_tag="VF589_06095"
     CDS             245..523
                     /locus_tag="VF589_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEX8257184.1"
                     /translation="MKIPLPYTPFPEEAQLIAALDSAAPRAAEAVAAEDFEAAMAALA
                     SLRAPVDAFFDKVTVNDPDPAKREARLALLARMRDAVHAVADFSRIEG"
     gene            704..3367
                     /gene="ppdK"
                     /locus_tag="VF589_06100"
     CDS             704..3367
                     /gene="ppdK"
                     /locus_tag="VF589_06100"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX8257185.1"
                     /translation="MTRYVYRFGGGVSEGGGGDRNLLGGKGANLAEMALIGLPVPPGF
                     TISTEMCARYYAEGESFPDSLREEVADGLAHIEAITGKRFGDAADPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDTTVAGLAATSGDPRFAWDSYRRFIQMYSDVVLGLDHGAFEEAL
                     EIAKEDKGVHLDTDLDARDWERLVGRYKELVEEESGRPFPQDVHEQLWGAVGAVFGSW
                     QSERAKVYRRLNSIPHDWGTAVNVQAMVFGNMGETSATGVAFTRDPSTGERAYYGEYL
                     INAQGEDVVAGIRTPQYLTRAARERAGAKAASMEESLPTVYAELAGVFDTLERHYRDM
                     QDIEFTVERGKLWMLQTRSGKRTAKAALRIAVDMAGEGLIAEEEAVLRVDPSGLDQLL
                     HPTLDPAAPRQVVAKGLPASPGAACGLAVFDADTAERYASLGEKVILVRIETSPEDIH
                     GMHAAKGILTCRGGMTSHAAVVARGMGRPCVSGAGSIQVDYKAKLMRIGRVEIRQDDT
                     ITIDGSTGEVMLGAVPTIQPELVGDFGTLMVWADKVRRLRVRANAETPNDCRTAREFG
                     AEGIGLCRTEHMFFDAERITAVRQMILAENEAGRRAALAKLLPAQRSDFEQIFRIMAG
                     LPVTVRLLDPPLHEFLPQSEEDFAEVASAVGISTDLLKRRVGELHEFNPMLGHRGCRL
                     GITYPEIYEMQARAIFEAAVAVARDSGEAPIPEVMVPLVGTKAELAIIRQLIDRTATA
                     VFAEQGASVDYLVGTMIELPRAALMAGSIAEEAQFFSFGTNDLTQTTLGVSRDDAGRF
                     LTQYVEKGIYARDPFVGIDEEGVGQLIALAAERGRATVPGLKLGICGEHGGDPASIAF
                     CEKTGLDYVSASPYRVPIARLAAAQAALRMR"
     gene            complement(3617..3850)
                     /locus_tag="VF589_06105"
     CDS             complement(3617..3850)
                     /locus_tag="VF589_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257186.1"
                     /translation="MTPFSSDQWVMLPLAFLLGVFVGMMFLSGGKWKRRYREEARLRA
                     EESKRRETLEADLKHSEARHIAAKARDDRPGPR"
     gene            complement(3921..4931)
                     /locus_tag="VF589_06110"
     CDS             complement(3921..4931)
                     /locus_tag="VF589_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8257187.1"
                     /translation="MTPPPAPLPGSPTIMGVDVMMVATVLAAVATFAVLIAIYAATTV
                     RDPMAKRVKALNERREQLKAGIVASTSKRRKKITNRNEAADKVRTLLSSLKMLQDEQV
                     EKAQTRLMQAGIRSKDLAFVVIFARFVLPAVFGTIMLVGVYLLDWFPDWGALKKYGLC
                     ATTLIGSYKAPDIWLKNKITKRTHAIRKGLPDALDLLVICAEAGLTVDAAFNRVAREL
                     GRAYPELGDEFALTSIELGFLTDRRLAFENLALRIDLESVRGVVTTMIQTEKYGTPLA
                     SALRVLSAEFRNERMMRAEEKAARLPAIMTVPLILFILPVLFIVILGPAACAISDNLM
                     TG"
     gene            complement(4948..5913)
                     /locus_tag="VF589_06115"
     CDS             complement(4948..5913)
                     /locus_tag="VF589_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8257188.1"
                     /translation="MFAIMMAAAGMGVITLLYFAFAGPSPAKAQVRRLESLRERHSKS
                     SEVVAQAQLKKIQSSRESRMDGIAKRFIPNPALLRRRLEQTGKSWTISQYAMASVGLG
                     LAALLLVKLQGGPFYLALAIGLLVGFAIPHFVVGKLFVRRVTKFTARFPDAIELMVRG
                     LRSGLPISETISIVAAEIAGPVAVEFQAVSDKMKIGRTMETALQEAADRLGTPEFQFF
                     VITLAIQRETGGNLAETLSNLADVLRKRAQMKLKIRAMSSESKASAYIIGALPFIVFG
                     LISYINPSYMGDFFVDERLIIAGLGGLVWMSIGALIMRKMINFEI"
     gene            complement(6001..7281)
                     /locus_tag="VF589_06120"
     CDS             complement(6001..7281)
                     /locus_tag="VF589_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HEX8257189.1"
                     /translation="MNAPFHAIRGATLRDPFTAFVCDEATAEMLRPIAVEHGWAPEKV
                     NKGGLRNAVQSLSVSASPNILFVDLSESGDPLNDINALAEVCEPGTVVIAAGQVNDVR
                     LYRDLVASGIQDYLLKPFSADQVRDAFANAQMTISGPRTNEPTTERPHIMAAVIGVRG
                     GVGASTIASSVAWLMGDKIGRSTALLDLDVHFGTGALSLDLEPGRGLTDAIENPSRID
                     GLFIERAMVRANDKLCVLSAEAPINQPLLSDGSAFFQLQEEMRNAFETTIIDLPRQML
                     VQHPHLVQDAHVSIVVAEFTLAGTRDAIRILSWLKANAPQSRVVVVANKAVAGTGQEI
                     SRKDFEKSIERKVDVVIPLDPKAAAQAAKLGQPLAKSASSPKIAQPLNQLVNLTVSAV
                     DGSDDKDAAEAGSSLLGKLNFKALVAKKPSAQAA"
     gene            complement(7281..>8102)
                     /locus_tag="VF589_06125"
     CDS             complement(7281..>8102)
                     /locus_tag="VF589_06125"
                     /inference="COORDINATES: protein motif:HMM:NF021025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEX8257190.1"
                     /translation="DRPGPGHTRSRAASGPAAARARARARAAGARRGPRRLRNSRLQL
                     LTMADARKESMIMARFKLSVALAAAAVAAAGCGPETNRLTPVSNPSLYSVNQPVVQRT
                     DFAFDVSSTGDGLPASERGRLGAWFESLRLGYGDRVSIDEASGYADARSRQDVADLAA
                     IHGLLLTEGAPITAGYVQPGTVRVIVSRTLASVPGCPNWEDEIVGAPERTATNYGCAT
                     NSNLAAMIADPSDLVLGQTSSGIDAADAAKTVQVYRNRVPSGINGKLKAESAGGK"
BASE COUNT         1283 a         2705 c         2801 g         1313 t
ORIGIN      
        1 tctcctcgcc ggctacaagc gcgccgccaa catcctcaag aaggaggggt ggcaaactcc
       61 agcaccgggt gacgatggcc cccggccatc gtcagatcgt gcggggcacg atcggcccgg
      121 tgctccccat gggggagagg gtcggggtga gggggagcga gcgtccagcg agcgtcctgc
      181 ctccgaagca tcttcccgaa cgtcggaccc cctcacccaa ccctctcccc gcgggggaga
      241 gggcttgaaa attccccttc cctacacgcc gtttcccgag gaagctcagc tcatcgccgc
      301 gctcgactcg gccgcgccgc gcgccgctga ggcggtggcg gcggaggatt tcgaggcggc
      361 gatggcggcg ctggccagcc tgcgcgcgcc ggtggacgca tttttcgaca aggtgaccgt
      421 caatgatccc gatcccgcca agcgcgaagc caggctcgcc ctcctcgccc gcatgcgcga
      481 cgcggtccat gcggtcgccg atttctcgcg catcgaaggc tgagcctcgc gcgcgcaccc
      541 acgcgcgcgc gcgagacccg ctgaccttgc tgaacttcgt ccggttccgg acaggcgccg
      601 gcgtcgcgcc gacgggtgac cggaccgatg gcggggacgc gccgtccccc ggacggtgcg
      661 ccagggggcc cgatcggcga gtgcggcgca acggagagtt tgaatgaccc gatatgtgta
      721 ccgcttcggc ggcggcgtct ccgagggcgg cggcggggat cgcaacctgc tcggcggcaa
      781 gggcgcgaac ctcgccgaga tggccttgat cggcctgccg gtgccgccgg gcttcaccat
      841 ctcgaccgaa atgtgcgccc gttattatgc cgaaggcgaa agcttccccg acagcctgcg
      901 cgaggaagtg gcggacggcc tcgcccacat cgaagccatc accggcaagc gcttcggcga
      961 cgccgccgat cccttgctgg tctcggtccg ctccggcgcc cgcgtgtcga tgccgggcat
     1021 gatggacacc gtcctcaatc tcggcctcaa cgacaccacg gtggcgggcc tcgccgcgac
     1081 ctccggcgat ccgcgcttcg cctgggacag ctatcgccgc ttcattcaga tgtattcgga
     1141 cgtggtgctc ggcctcgatc acggcgcgtt cgaggaagcg ctcgagatcg ccaaggagga
     1201 taagggcgtc catctcgaca ccgatctcga cgcccgcgat tgggagcggc tggtcggccg
     1261 ctacaaggag ctggtcgagg aagaaagcgg ccggccgttc ccgcaggacg tgcacgagca
     1321 attgtggggc gcggtcggcg ccgtgttcgg ctcgtggcag tcggagcgcg ccaaggtcta
     1381 ccggcgattg aattcgatcc cgcacgattg gggcaccgcg gtcaacgtcc aggcgatggt
     1441 gttcggcaat atgggcgaga cctcggccac cggcgtcgcc ttcacccgcg acccctcgac
     1501 cggcgagcgc gcttattatg gcgaatatct gatcaacgcc caaggcgagg acgtcgtcgc
     1561 cggcatccgc accccgcaat atctgacccg cgccgcgcgc gagcgggccg gcgccaaggc
     1621 ggcctcgatg gaggaatcgc tccccaccgt ctatgccgag ctcgccggcg tgttcgacac
     1681 gctcgagcgc cattatcgcg acatgcagga catcgagttc accgtcgagc gcggcaagct
     1741 ctggatgctg cagacgcgct cgggcaagcg caccgccaag gccgcgctca ggatcgcggt
     1801 cgacatggcc ggcgaggggc tgatcgccga ggaggaggcg gtgcttcgcg tcgacccgtc
     1861 cggcctcgac cagttgctcc acccgacgct cgatccggcc gcgccgcgcc aggtcgtcgc
     1921 caaggggctg cccgcctcgc ccggcgccgc ctgcggcctg gccgtgttcg acgccgacac
     1981 cgccgaacgc tacgcctcat tgggcgagaa ggtgatcctg gtccggatcg agaccagccc
     2041 cgaggacatt cacggcatgc acgccgccaa gggcatcctc acctgccgcg gcggcatgac
     2101 cagccacgcc gccgtggtcg cgcgcggcat gggccgtccc tgcgtcagcg gcgcgggctc
     2161 gatccaggtc gactataagg ccaagctgat gcggatcggc agggtcgaga tccgccagga
     2221 cgacaccatc accatcgacg gctcgactgg agaagtgatg ctcggcgccg tcccgaccat
     2281 ccagcccgag ctggtcggcg atttcggaac cctgatggtc tgggccgaca aggtccggcg
     2341 gcttcgggtc cgcgccaatg ccgagactcc gaacgactgc cgcaccgccc gcgaattcgg
     2401 cgccgagggc attggcctgt gccgtaccga gcatatgttc ttcgatgccg agcgcatcac
     2461 cgcggtccgc cagatgatcc tggccgagaa cgaggccggc cgccgcgccg ctctggccaa
     2521 gctgcttccg gcgcagcgca gcgacttcga gcagatcttc cggatcatgg ccgggctgcc
     2581 ggtcaccgtc cgcctgctcg atccgccgct ccacgaattc ctgccgcaaa gcgaggagga
     2641 tttcgccgag gtcgcgtcgg cggtcggaat ttctaccgac ctcctcaagc gccgcgtcgg
     2701 cgagctccac gaattcaacc cgatgctcgg ccatcgcggc tgccggctcg gcatcaccta
     2761 tcccgaaatc tacgagatgc aggcgcgcgc catcttcgag gcggcggtcg cggtggctcg
     2821 ggacagcggc gaggccccca ttcccgaggt gatggtgccg ctggtcggga ccaaagccga
     2881 gctcgccatc atccgccagc tgatcgaccg cacggcgacc gcggtgttcg ccgagcaggg
     2941 cgcgtcggtc gattatctgg tcggcaccat gatcgagctg ccgcgcgccg ctttgatggc
     3001 cggcagcatc gccgaggagg cgcaattctt cagcttcggc accaacgacc tcacccagac
     3061 cacgcttggc gtcagccgcg acgatgccgg ccgcttcctc acccaatatg tcgaaaaggg
     3121 catttacgcc cgcgacccgt tcgtcggcat cgacgaggag ggggtcggcc agctgatcgc
     3181 gctggccgcg gagcggggca gggcgacggt gccgggcctc aagctcggca tatgcggcga
     3241 gcatggcggc gatccggcct cgatcgcctt ttgcgagaag actggcttgg attacgtctc
     3301 ggcgtccccc taccgggtgc cgatcgccag actcgcggcc gcccaggcgg cgttgcggat
     3361 gcggtgaggg gggatgggag atgaaggcgg ttcaagccat ctccgctcat cccgaggagg
     3421 cgctgagctt gtcgaagcgg cgtctcgaac gacgcaccct cgcccgttcc ccggcgaagg
     3481 ccggggccca gactccagcg cagctggacc ccggccttcg ccggggaacg gaggggggat
     3541 ggtccttcga gacgggcctt cgccttcgct cagtccctcc tcaggatgag cggttgtggt
     3601 tacgactaac ttaccctcag cgtggacccg gccggtcatc ccgcgccttg gccgcgatat
     3661 gccgggcctc ggaatgcttg aggtcggcct ccagcgtctc gcgccgcttg ctctcctcgg
     3721 cccgcagccg cgcctcctcg cggtaccggc gcttccactt gccgccggac aggaacatca
     3781 tgccgacgaa cacaccgagc aggaaggcga gcggcagcat cacccactgg tccgagctga
     3841 atggtgtcac cggctactcc ttcgacaaac tgcttcaggt taaacaatcg gcgaaggggg
     3901 agggttgttc cgcctccgga ctaaccggtc atcagattgt cggagatggc gcaggcggcc
     3961 gggcccagga tgacgatgaa cagcaccggc aggatgaaca gaatgagcgg cacggtcatg
     4021 atcgccggca gccgcgcggc cttttcctcg gcgcgcatca tgcgctcgtt gcggaactcg
     4081 gccgacagca cgcgcagcgc gctcgccagc ggagtgccgt atttctcggt ctggatcatg
     4141 gtcgtgacga cgccgcgcac cgattcgagg tcgatgcgca aggcgaggtt ctcgaacgcc
     4201 agccgtcggt cggtgaggaa gcccaattcg atcgaggtca aagcgaattc gtcgccgagc
     4261 tccggatagg cccggccgag ctcgcgcgcg acccggttga aggcggcgtc gacggtgagg
     4321 ccggcctcgg cgcagatcac cagcaggtcg agcgcgtcgg gaaggccctt gcggatcgcg
     4381 tgggtgcgct tggtgatctt gttcttgagc cagatgtccg gcgccttgta gctgccgatt
     4441 aaggtggtcg cgcacaggcc gtatttcttg agcgcccccc agtccgggaa ccagtcgagc
     4501 agatagacgc cgaccagcat gatcgtgccg aacaccgccg gcagcacgaa gcgcgcgaag
     4561 atgacgacga aggcgaggtc tttggagcgg atgccggcct gcatcagccg cgtctgcgcc
     4621 ttctcgacct gctcgtcctg gagcatcttc agggagctca gcagcgtgcg caccttgtcg
     4681 gccgcctcgt tgcgattggt gatcttcttg cgccgcttcg aggtcgaggc gacgatgccc
     4741 gccttcaatt gctcgcgccg ctcgttgagc gccttgacgc gcttggccat cgggtcgcga
     4801 accgtggtcg ccgcatagat ggcgatcagc acggcgaagg tggcgacggc ggcgagcacg
     4861 gtcgccacca tcatcacgtc gacgcccatg atggtgggtg agccgggaag aggagcggga
     4921 ggcggtgtca tgtcgaaaga tcgctcctca aatctcgaaa ttgatcatct tgcgcatgat
     4981 gagggcgccg atgctcatcc acacgaggcc gccgaggccg gcgatgatca gccgctcgtc
     5041 gacgaagaag tcgcccatgt agctggggtt gatgtaggag atcaggccga agacgatgaa
     5101 cggcagcgcg ccgataatgt aggcggacgc cttggattcc gacgacatgg cgcggatctt
     5161 gagcttcatc tgggcgcgct tgcgcagcac gtcggcgagg ttggacagcg tctcggcgag
     5221 gttgccgccg gtctcgcgct ggatcgccag cgtgatgacg aagaactgga attcgggcgt
     5281 gccgagccgg tcggccgctt cctggagggc agtctccatc gtgcggccga tcttcatctt
     5341 gtccgacacc gcctggaact cgaccgccac cggccccgct atctccgccg cgacgatcga
     5401 gatcgtctcg gaaatgggaa ggcccgagcg cagtccgcgc accatcagct cgatggcgtc
     5461 cgggaagcgc gcggtgaact tggtgacgcg gcgcacgaac agcttcccga ccacgaagtg
     5521 ggggatggcg aagccgacca gcaggccgat cgccagcgca agatagaagg ggccgccctg
     5581 cagcttcacc agcagcaaag cggcgaggcc gaggccgacc gaggccatcg catattggct
     5641 gatcgtccag ctcttgccgg tttgctcgag ccgccggcgg agcagcgccg gattggggat
     5701 gaagcgcttg gcgatgccgt ccatccggct ttcgcggctg gactggatct tcttcaactg
     5761 cgcctgcgcg accacttcgc tcgatttgct gtggcgctcg cgcaggctct cgagccggcg
     5821 gacctgggcc ttggcggggg aggggccggc gaaggcgaag taaaggagcg tgatcacgcc
     5881 cattcccgcc gccgccatca tgatcgcaaa catgtcgtct ctcgcccctt gctgtgcacc
     5941 accgccgccg ccgcggcggc ctctccccgg gtcaggccgc cgcggcggcc ggctcgcggg
     6001 tcaggccgcc tgcgccgacg gcttcttggc caccagcgcc ttgaagttga gcttgccgag
     6061 cagcgaggag ccggcctcgg ccgcgtcctt gtcgtcgctg ccgtcgaccg ccgagacggt
     6121 gaggttgacg agctggttca gcggctgcgc gatcttcggc gaggaagccg atttcgcgag
     6181 cggctggccg agcttcgccg cctgcgcggc cgccttgggg tcgagcggga tcaccacgtc
     6241 gaccttgcgc tcgatcgatt tctcgaagtc cttgcggctg atttcctggc cggtcccagc
     6301 aaccgcctta ttggccacca ccaccacccg cgactgcggc gcgttggcct tgagccagga
     6361 caggatccgg atcgcgtcgc gtgtgccggc gagcgtgaac tccgccacca cgatcgagac
     6421 atgggcgtcc tggacgagat gcggatgctg gaccagcatc tgccgcggca ggtcgatgat
     6481 cgtcgtctcg aacgcgttgc gcatttcctc ctgcagctgg aagaaggcgg agccgtcgct
     6541 gagcagcggc tggttgatcg gcgcttccgc cgacaggacg caaagcttgt cgttcgcgcg
     6601 gaccatcgcc cgctcgatga acagcccgtc gatgcggctc ggattctcga tcgcgtcggt
     6661 cagcccgcgg cccggctcga ggtcgagcga gagcgcgccg gtgccgaaat ggacgtcgag
     6721 gtcgagcaag gccgtcgagc ggccgatctt gtcgcccatc agccacgcca ccgacgaggc
     6781 gatggtcgag gcgccgacgc cgccgcggac gccgatcacc gccgccatga tgtgcggccg
     6841 ctcggtggtg ggctcgttgg tgcgcggccc ggatatggtc atctgggcgt tggcgaaggc
     6901 gtcgcggacc tggtcggcgc tgaacggctt cagcagataa tcctggatgc cgctggcgac
     6961 gaggtcgcgg tagagccgga cgtcgttgac ctggccggcg gcgatcacca ccgtgccggg
     7021 ttcgcagacc tcggcgagcg cgttgatgtc gttgagcggg tcgcccgatt cggacagatc
     7081 gacgaacagg atgttcgggc tcgccgagac cgacagcgac tggaccgcgt tgcgcagccc
     7141 gcccttgttc accttctccg gcgcccagcc atgctcgacc gcgatcgggc gcagcatttc
     7201 ggccgtggct tcgtcgcaga cgaaggcggt gaaggggtcg cggagcgtgg ctcccctaat
     7261 ggcgtggaat ggagcgttca ttatttgcca cctgctgatt cggctttgag cttcccgttg
     7321 attccgctcg gcacccggtt gcggtacacc tgcacggtct tggccgcgtc cgcggcatcg
     7381 atgccgctcg aagtctggcc gagcaccagg tcgctggggt cggcgatcat cgcggcaagg
     7441 ttcgaattgg tcgcgcagcc atagttggtc gcggtgcgct cgggggcgcc gacgatttcg
     7501 tcttcccaat tcgggcagcc gggcacgctg gccagggtgc ggctgacgat cacccgtacg
     7561 gtgccgggct ggacgtagcc cgccgtgatt ggggcgcctt cggtcagcag gaggccgtgg
     7621 attgccgcga ggtcggcgac atcctggcgg ctgcgagcat cggcatagcc ggaggcctcg
     7681 tcgatcgaca cgcggtcgcc atagccgagc cgaaggctct cgaaccaggc gccgaggcgg
     7741 ccacgctccg aagccggcaa cccgtcgccg gtggaagaca cgtcgaacgc gaagtcggtg
     7801 cgctgcacga ccggctggtt gaccgaatag aggcttggat tgctgaccgg ggtcaggcgg
     7861 ttcgtctccg ggccgcagcc ggcggcggcg acagccgcgg cggcgagagc gacggagagt
     7921 ttgaagcgtg ccatgatcat gctttccttt cgtgcgtcgg ccatggtcag aagctgaagc
     7981 cgggagttgc ggaggcggcg ggggccgcgg cgcgcgcctg ccgcacgggc acgggcacgg
     8041 gcgcgggctg cggccgggcc gcttgccgca cgggagcggg tgtggccggg gccgggacga
     8101 tc
//
LOCUS       DASVRK010000158         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_279015, whole genome shotgun sequence.
ACCESSION   DASVRK010000158 DASVRK010000000
VERSION     DASVRK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279015"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..615
                     /locus_tag="VF589_06130"
     CDS             145..615
                     /locus_tag="VF589_06130"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8257191.1"
                     /translation="MSGVIANLLVALLAAALLVAAAGDLRNRTIPNRLTGAVALLAIP
                     FWWFAGLTLWPDVIFQVCAAAAVFLLFALAFQLGAMGGGDVKLVSALALWLPPLAVMK
                     LLVIMSVAGGVLTLAMLVRHRLRKREEQLEIPYGVAIAFGGFWLISERYLYQFG"
     gene            659..1720
                     /gene="cpaB"
                     /locus_tag="VF589_06135"
     CDS             659..1720
                     /gene="cpaB"
                     /locus_tag="VF589_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX8257192.1"
                     /translation="MDVKKLVLLIGALVIAAVTAVMAKNMFSGAGAPEASAKAVVPAG
                     PEVLVATRALPVGTIIDAEALKFQMWPEGLVQPAYFIKGKPGVNPADLLGTVVRNEIT
                     AGQPLTMGALIRPGERGFLAAALGPGMRAVTVSVSDTAGVAGFVFPGDRVDIVLTQEV
                     SGDGEPLKVSETILRNVRVLATDQRTNVKNAEGRPVVKEISMVTLEATPKIAEKIAVA
                     QTIGKLSLALRSIADTTAELERAIAAGEVEVPDGTDPAAERKMLLAIATKPVDSGTTY
                     TVGADVSRFERSSVPGKAKPDEKVDAVNQMSKAIGSLAGAIGGPGPGAAAAPAPAPTG
                     PVVRVARGNNVTVVPVGGK"
     gene            1722..>3200
                     /locus_tag="VF589_06140"
     CDS             1722..>3200
                     /locus_tag="VF589_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX8257193.1"
                     /translation="MTKSNKFKRAALGTAIAAVIAAGIAPAVSAPALAQPSGSVKPTH
                     DVALSVGTGRLVQLDGTMSDLFVANDGIADVQVRSSNQIYIFGKAGGETTIYATSKAG
                     KVIYSANVRVGTNIGSVDEMLDLAMPDAQIRATSMNGMLLLTGTVATPADVEEAGRLV
                     QAFVGEGTRVVSRLKTATPLQVMLKVKIAEVSRSLARDVGVNLLTRDNTGGFLFGVGR
                     GNPGSITDVVSKTLTDPITGNPAAVGTGFKFRNPGGMTTLGFAGNLLGVDILSTLNLN
                     EASGLVTTLAEPTLTALSGETASFLAGGEFPIPTAQGINGTSVEFKEYGVSLAFTPVV
                     LEGGRISMRVRPEVSEISSAGAVKLNGFEIPALTTRRAETTVELGSGQSFMIGGLLRN
                     NGNNSVEKAPFLGDIPILGALFRSNSFRRNETELVIVVTPYLVKPVSASQIALPTDGY
                     RHSNDAQRILLDQREDGRSGERRPEPVSGPPQTVAPGIGALGS"
BASE COUNT          526 a         1108 c         1069 g          497 t
ORIGIN      
        1 gtccatgcgg cggtgggccc agccgtcggg cgaacgccag agcgacaggg aagcgcccgc
       61 gggccggctg cgccggtcga tgtccaccgt tttcatgggc gatggttacg gtttggcaag
      121 caagcccgtc tagagccgtc aaccatgtcg ggggtcattg ccaatcttct ggtcgcattg
      181 ctcgccgctg ccctgctcgt ggcggcggcg ggagacctgc gcaaccgcac cattccgaac
      241 cgcctcaccg gggcggtggc gttgctcgcc atccccttct ggtggttcgc cgggctgact
      301 ttgtggcccg acgtgatttt ccaggtctgc gccgcggccg ccgtgttcct gctgttcgcg
      361 ctcgctttcc agctcggcgc gatgggcggc ggcgacgtca agctggtgag cgcgctggcg
      421 ctgtggctgc cgccgctcgc ggtgatgaag ctgctcgtca tcatgtcggt ggcgggcggc
      481 gtgctcacgc tggcgatgct ggtccggcac cggctgcgca agcgcgagga gcaactcgag
      541 attccctacg gcgtagccat cgcctttggc ggtttctggc tgattagcga acgatatctt
      601 taccaatttg gatgatgagt agggcgggtt cgaagccctg caggaggcga ctagcgtcat
      661 ggatgtcaaa aagcttgtgc tactgatcgg cgcgctcgtt atcgcagcgg taaccgctgt
      721 gatggcgaag aacatgttca gcggggcggg ggctccggaa gcgtccgcca aggcggtcgt
      781 ccctgcgggg ccggaagtgc tggtcgcgac ccgcgcgctg cccgtcggca ccatcatcga
      841 cgccgaggcg ctgaagttcc agatgtggcc cgaggggctg gtccagccgg cctatttcat
      901 caaggggaag cccggcgtga acccggccga cctgctcggc accgtggtcc gcaacgagat
      961 cacggccggc cagccgctga ccatgggcgc cctcatccgc cccggcgaac gcggcttcct
     1021 cgccgcggcg ctcgggcccg gcatgcgcgc cgtgacggtc agcgtttccg acacggccgg
     1081 cgtcgccggc ttcgtgttcc ccggcgatcg tgtcgacatc gtgctgacgc aggaagtcag
     1141 cggcgacggc gaaccgctca aggtctccga gaccatcctg cgtaacgtcc gcgtcctcgc
     1201 caccgaccag cgcaccaacg tcaagaacgc ggaaggccgg ccggtcgtga aggagatctc
     1261 gatggtgacg ctggaagcga cgccgaagat cgccgagaag atcgcggtcg ctcagacgat
     1321 cggcaagctg tcgctcgcgc tgcgttcgat cgccgacacc acggccgagc tcgaacgcgc
     1381 catcgccgcc ggcgaggtcg aggtgcccga cggcaccgat ccggccgccg agcgcaagat
     1441 gctgctcgcc atcgctacca agccggtcga cagcgggacc acgtatacgg tcggcgccga
     1501 cgtctcgcgc ttcgagcgca gcagcgtccc cggcaaggcc aaacccgacg agaaggtcga
     1561 tgccgtcaac cagatgagca aggcgatcgg atcgctggcc ggcgctatcg gcgggccagg
     1621 tccgggagcg gccgccgcac cggcgcccgc tccgaccggg ccggtcgtgc gggtcgcccg
     1681 cggcaacaac gtcaccgtcg tcccggtggg aggcaagtaa gatgaccaag tcgaataaat
     1741 tcaagcgcgc ggcgctcggt accgccatcg cggcggtgat cgcagcgggg atcgccccgg
     1801 ccgtgtccgc ccctgccctg gcgcagccca gcggctcggt taagcccacc cacgacgtgg
     1861 cactctcggt cggcaccggc aggctggtcc agctcgacgg caccatgtcg gacctgttcg
     1921 tcgccaatga cggcatcgcc gacgtccagg tccgctcgtc caaccagatc tacatcttcg
     1981 gcaaggccgg gggcgagacc acgatctacg cgaccagcaa ggccggcaag gtgatctatt
     2041 cggccaacgt ccgcgtcggc accaatatcg gctcagtcga cgagatgctg gacctcgcca
     2101 tgcccgacgc gcagatccgc gccacctcga tgaacggcat gctgctgctc accggcacgg
     2161 tcgccacgcc ggccgacgtc gaagaagcgg gccggctcgt ccaggccttc gtcggcgaag
     2221 gcacgcgggt ggtcagccgg ctcaagacgg cgacgccgct gcaggtgatg ctgaaggtca
     2281 agatcgccga ggtcagccgc tcactcgccc gcgacgtcgg cgtcaacctg ctcactcgcg
     2341 acaacacggg cggcttcctg ttcggcgtcg gccgcggcaa ccccggctcg atcacggacg
     2401 tcgtctccaa gacgctcacg gacccgatca ccggcaaccc ggcggccgtc ggcaccggct
     2461 tcaagttcag gaatccgggc ggaatgacga cgttgggctt cgccggcaat ctgcttgggg
     2521 tcgatatcct ctcgacgctc aacctcaacg aggcgagcgg gctcgtcacg acgttggccg
     2581 aaccgacgct gaccgcgctc tccggcgaga ccgccagctt cctggccggc ggcgagttcc
     2641 caatcccgac cgcgcaaggc atcaacggca cctcggtcga gttcaaggaa tatggcgtca
     2701 gcctggcctt cactccggtg gtactcgaag gcggccgcat ctcgatgcgg gtccgtcccg
     2761 aagtctccga aatctcctcg gccggcgcgg tgaagctcaa cgggttcgaa atcccggcgc
     2821 ttaccacccg cagggcggag accacggtgg agctcggctc cggccagagc ttcatgatcg
     2881 gcggactgct tcggaacaac ggcaacaatt cggtcgaaaa ggcgcccttc ctgggcgaca
     2941 tcccgatcct cggggcgctg ttccggtcga acagcttccg gcgcaacgag accgagctgg
     3001 tgatcgtggt cacgccctac ctggtgaagc cggtcagcgc gagccagatc gcgctcccga
     3061 cggacggcta tcggcacagc aacgacgccc agcggatcct gctcgaccag cgcgaggacg
     3121 gccgtagcgg cgaacgccgg cccgagcccg tcagcggccc accccagacg gtcgctcccg
     3181 gcatcggcgc ccttggcagc
//
LOCUS       DASVRK010000159         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_147209, whole genome shotgun sequence.
ACCESSION   DASVRK010000159 DASVRK010000000
VERSION     DASVRK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147209"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF589_06145"
     CDS             complement(<1..313)
                     /locus_tag="VF589_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8257194.1"
                     /translation="MTKSALVCGAGGFIGSHLVKRLKREGFWVRGVDLKYPEFAETQA
                     DDFMIGDLRDQNVCDRVVDRRFDEVYQLAADMGGAGYIFTGEHDADIMHNSATINLNV
                     LD"
     gene            complement(310..1518)
                     /locus_tag="VF589_06150"
     CDS             complement(310..1518)
                     /locus_tag="VF589_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8257195.1"
                     /translation="MRIIVSDYSGHPFQVQLSRALAGLGHEVLHLYSASFQTPKGDLL
                     RRDDDPAGFAITGVKSAQPFAKDSFVKRRSQEIEMGGLIAAEIEKFAPDVVFSSNAPI
                     DTQVRIRRAARKAGARFVFWVQDLYGEAIWRILRGKFGPFGDVVGRFYSGMERRLIRS
                     SHHVVAIADEFKIQVCRQTGIPADKVTVIENWAPLAEVPLAPRDNEWAEANLPASPVR
                     FIYSGTLGFKHDPGLLLKIARSVSGHVIVFSEGKAAESLAQQAKAEGVANLEVRSWLP
                     FTDLPKALAGGDVLIVVLEPDAGIFSVPSKVLTYLCAGRPILGSVSKANLAARIIAGA
                     GAGIVEEPGDYDALIAAARRLEADPAERAQMGAKARAYAEKTFDIDTIAGKFANIAAR
                     LAQPTSGDIK"
     gene            complement(1703..>2315)
                     /locus_tag="VF589_06155"
     CDS             complement(1703..>2315)
                     /locus_tag="VF589_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8257196.1"
                     /translation="PLGLRDRVAKRALDLAIALPATLLLLPLMLVVGIAIKLESAGPV
                     FFSQPRVGRGNRIFHVLKFRSMRADTADSAGVRSTARGDDRITRIGAFIRRTSIDELP
                     QLINVVLGDMSIVGPRPHALASTAEDQLFWAIDPRYWDRHAIKPGMTGLAQIRGYRGA
                     TSCLTDLTNRLQADLEYMSGWSMGRDLAIIFRTLRVMAHRNAF"
BASE COUNT          386 a          732 c          815 g          382 t
ORIGIN      
        1 ggtcgaggac gttcaaattg atcgtcgccg aattgtgcat gatgtcggcg tcatgctcgc
       61 cggtgaagat gtaccccgcg ccgcccatgt cggcggcgag ctggtagacc tcgtcgaagc
      121 ggcggtcgac gacgcggtcg cagacattct ggtcgcgaag gtcgccgatc atgaagtcgt
      181 cggcctgggt ctcggcgaat tcgggatatt tgaggtcgac gccgcggacc cagaagccct
      241 cgcgcttcag ccgcttcacg agatggctgc cgatgaagcc gccggccccg cagaccagag
      301 cagatttcgt catttgatgt ctccagatgt gggctgcgcg aggcgagccg ctatgtttgc
      361 aaactttccg gctatggtat cgatgtcaaa ggttttttcg gcataggccc gggctttggc
      421 gcccatttgc gcgcgctcgg ccgggtcggc ctccagccgc ctggcggccg cgatcagcgc
      481 gtcgtaatcg cccggctcct cgacgatccc ggcgccggcg ccggcaatga tgcgcgcggc
      541 gaggttggcc ttcgataccg agccgaggat cggccgcccg gcgcacagat aggtcaggac
      601 cttggacggc accgagaaga tgccggcgtc cggctccagc acgacgatca gcacgtcgcc
      661 gccggccagg gccttgggaa ggtcggtgaa gggcagccag ctgcgcacct cgagattggc
      721 gacgccctcg gccttggcct gctgcgcgag cgattccgcc gccttgccct cggaaaagac
      781 gatgacgtgg ccgcttacgc tccgcgcgat cttcaggagc aggccggggt cgtgcttgaa
      841 cccgagcgtg cccgaataga tgaagcgcac gggcgaggcc ggcagattgg cctcggccca
      901 ctcattgtcg cgcggcgcca gcggcacttc ggccagcggc gcccaattct cgatcacggt
      961 caccttgtcg gccgggatgc cggtctgacg gcagacctgg attttgaatt cgtcggcgat
     1021 cgccacgaca tggtgggacg agcggatcag gcggcgctcc atgccgctgt agaaccggcc
     1081 gacgacgtcg ccgaacgggc cgaatttgcc gcgcaggatg cgccagatcg cctcgccgta
     1141 aaggtcctga acccagaaga cgaagcgcgc gccggccttg cgcgcggcgc ggcggatccg
     1201 cacctgcgtg tcgatcggcg cgttgctcga aaagacgacg tccggcgcga acttttcgat
     1261 ctcggcggcg atcaggccgc ccatctcgat ctcctggctg cggcgcttga cgaagctgtc
     1321 cttggcgaag ggctgggccg atttcacccc ggtgatggcg aagccggcgg ggtcgtcgtc
     1381 ccggcgcagc aaatcgccct tcggggtctg gaagctggcc gaatagagat gcagcacctc
     1441 atggccaagg ccggccaggg cgcgcgagag ctgcacctgg aagggatggc ccgaatagtc
     1501 gctgacgatg atccgcactg gccgctttcg tgaaaatgaa gtctcagctc cggcggccgg
     1561 ttgccgcgcg ggcatggttc gtccggacca tgatcagtcc cgcgagcccc actgccgctc
     1621 gcggggcaaa gtccccgtat ggctcgccga aataaagctg tcccttgtgc ggcgcaacat
     1681 cataatcgct ggaaggccgg gttcagaagg cgttgcgatg cgccatcacg cgaagggtgc
     1741 ggaagatgat cgcaagatcg cggcccatgc tccaccccga catatattcg agatcggcct
     1801 gcagccggtt ggtgaggtcg gtgaggcagg aggtggcgcc gcgatagccg cgtatctggg
     1861 cgaggcccgt catgcccggc ttgatcgcat ggcggtccca gtagcgcggg tcgatcgccc
     1921 agaacaattg gtcctcggcg gtcgaggcga gcgcgtgggg gcgcgggccg acgatgctca
     1981 tgtcccccag caccacgttg atcagctgcg gcagctcgtc gatgctggtg cggcggatga
     2041 acgcgccgat ccgggtgatg cggtcgtcgc cgcgggcggt cgaacggacg cccgccgaat
     2101 cggcggtgtc ggcccgcatg ctgcggaact tgagcacgtg gaagatgcgg ttgccgcgcc
     2161 cgacccgggg ctggctgaag aaaaccggcc ccgccgattc gagcttgatg gcgatgccga
     2221 ccaccagcat cagcggcagc agcagcaggg tggcgggcag cgcaatggcg aggtcgagcg
     2281 cgcgcttggc gacgcggtcg cgaagcccga gcggg
//
LOCUS       DASVRK010000160        10786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_527576, whole genome shotgun sequence.
ACCESSION   DASVRK010000160 DASVRK010000000
VERSION     DASVRK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10786
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527576"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..699
                     /locus_tag="VF589_06160"
     CDS             217..699
                     /locus_tag="VF589_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8257197.1"
                     /translation="MATAHLLFGYLGSGKTTFAKRLEVEHAAVRFTPDEWMARLFGDD
                     PPEESFPEKAEAILELLEPLWTRCLSLGLDVVLDYGFWTRKERDEVRAVVERLGASAC
                     LYRLGCPDDEARRRIDARNQADDRSLYIAPATYNALWRRFEPLDVDEAFVEVDNRTVR
                     "
     gene            complement(813..1724)
                     /locus_tag="VF589_06165"
     CDS             complement(813..1724)
                     /locus_tag="VF589_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8257198.1"
                     /translation="MARGVFIHRADSIYEDHPETQYQFPAMYLDRAARFVGDWILYYE
                     PAKVRNSRGYFAIARVERIVPDPTKSAMHLALIEPGSYLSFERPVPFTGPEGRPERGV
                     VNPQWAVRPVSLEDFNRILDLGIPDEAPLLPRAGTPAENPAYVEPSRDTPGLVREEQA
                     PFQVDDAERERVGYYSSRIVRDRVFRRIVLDAYDSRCAVTGLKFINGGGRAEVEAAHI
                     KPVETNGPDDIRNGLALSGTAHWMFDRGLISLSDDLDVLVSRQVNDVEGVWKLVNQAR
                     RAAVPGNPAHRPHPSYLAWHRERCFKR"
     gene            2121..2579
                     /locus_tag="VF589_06170"
     CDS             2121..2579
                     /locus_tag="VF589_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8257199.1"
                     /translation="MISQKTRYALRSLLYLVEEGKGAPVQLARIAETQQVPRKYLELI
                     MLELKKAGIVASVRGPAGGYRLARAPEEVSFGDVIRIMDGPIALVPCASVHFYSTCGD
                     CHDEATCAIRKVLAMVRESSAGILEGTSLAQAAGWETGLSAPEGASSAMS"
     gene            2646..3701
                     /locus_tag="VF589_06175"
     CDS             2646..3701
                     /locus_tag="VF589_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8257200.1"
                     /translation="MPSLPKLPKLPKLPSLKHVAAAAALAATALAPPAALGGAAQAQL
                     LNVSYDPTRELYRDINANFTRRWKAASGQNVTIRQSHGGSGKQARSVIDGLQADVVTL
                     ALAYDIDEISSRAKALPANWQSRLPNNSSPYYSTIVFLVRKGNPKKIADWNDLIRPDV
                     KVITPNPKTSGGARWNYLAAWGYAQKKYGSPAKAREFLTRMFKNVPVLDSGARGATTT
                     FVQRGQGDVLLAWENEAFLAVNQLGRGKFDIVVPSMSILAEPPVALVDKVVDKKGTRK
                     AAEAYLKYLYTPEAQEIIAKHYYRPRSPQVAAKYKARYPNVALLTIKNFGGWGRAQAT
                     HFNDGGLFDQIFEAAKR"
     gene            3791..5254
                     /locus_tag="VF589_06180"
     CDS             3791..5254
                     /locus_tag="VF589_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX8257201.1"
                     /translation="MRLFLIAGVSLIALAAPAQAQEPTDESLSEAPSGGTIDTIIQDE
                     APAAAPAPTGDPVLDRLNALEAKVNQLERRNAELEQQAELNQGRIESVEARAGKNVQF
                     SWGPTFADTSGGFTFKPRGVVEADYAAFFEREGGYDFNNGTGFRRARIGFEGTAFKKF
                     NYRIEADFAGNTVNLTDAYLQYVASPQFLITAGQHKAPFGLESNNSDNYNIFMERGMF
                     TNAFGNAGAERRIGLSAAYQTETLNAAAGIFGENESIGRSSATPVDDTPDESWGFNGR
                     IAWEPIFEPEKILHLGVSGYHRTALRSGDVEDAVRFNDRPNIRIDNGNIADSGVITGV
                     ESLRYLGGEAAAVFGPVTLAGEYGRVWVDRPEADNLSFDGFYLYGTWFITGESRPFRN
                     GNFDRVKPFTSLGQDGVGALELALRYDKINLGDTPVEARIGNRAESLTMGLNWYFNPY
                     AKLLFNWVRFDGVNTPLDPESDRTEGDVFATRLHLDF"
     gene            5283..5714
                     /locus_tag="VF589_06185"
     CDS             5283..5714
                     /locus_tag="VF589_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257202.1"
                     /translation="MRCTALALLLSASLLAGCSGGDDKQGGASVEILNVSYDPTRELY
                     KEVNAAFARQWQGQTGQQVSFNMSHGGSGKQSRAVIDGLEADVVTLALAYDIDALPRR
                     RSGCRPIGRRGCRTTARLISRRSYSSSARAIPGASAAGATS"
     gene            5621..6304
                     /locus_tag="VF589_06190"
     CDS             5621..6304
                     /locus_tag="VF589_06190"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8257203.1"
                     /translation="MPNNSAPYFSTIVFLVRKGNPRGIRGWGDLVKPGVKVITPNPKT
                     SGGARWNFLAAWGYGRKAGGSDAAAEQYVSGLLGNVPVLDSGARGSTTTFVERGIGDV
                     LLAWENEAFLAQEELGQGKFEIVVPSMSILAEPPVAVIDQNAAKHGTQAVAQAYLNFL
                     YTRPAQEMIARHFYRPRDPQVAAQHAARFPRIELLEIDRDFGGWKAAQARYFDDGGAF
                     DTIYARIKR"
     gene            6301..7218
                     /gene="cysT"
                     /locus_tag="VF589_06195"
     CDS             6301..7218
                     /gene="cysT"
                     /locus_tag="VF589_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX8257204.1"
                     /translation="MTRNMSAAAMPIEPPIAVSQGYREARGRRPRKVRKSIIPGFGLT
                     FGFTLAYLCLIVLIPLSAVFLKTAGMGWAEFVKVGLSDRALAAYRLSFGAAFLAATVN
                     ALFGLNIAWMLVRYDFPGKRVVNALVDLPFALPTAVAGIALTALYADTGWIGQYLAPL
                     GIQVAFKPLGVVIALIFVGLPFVVRSVEPVLADVGVDVEEAAASLGANRLQTFRRVLL
                     PAILPALLTGFALAFARGVGEYGSVIFIAGNMPFESEIAPLLIVTQLEQYSYTGATAI
                     AVVMLVVSFAILLVINLIQAWSRRRSIDR"
     gene            7215..8054
                     /gene="cysW"
                     /locus_tag="VF589_06200"
     CDS             7215..8054
                     /gene="cysW"
                     /locus_tag="VF589_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX8257205.1"
                     /translation="MTSRAASESRFARKLLIGTALGFLGLFVVLPLLAVFVEALREGF
                     GPSLESVKEPDALSAVRLTLTVAAISVPLNIVFGLAAAWSITKFDFPGKSILTTLIDL
                     PFSVSPVVSGLIYVLLFGAQGWLGDWLAARDVQIIFAVPGIVLATVFVTFPFVARELI
                     PLMAEQGRDDEEAALSLGASGWQTFRRVTLPNIRWGLLYGVLLCNARAMGEFGAVSVV
                     SGHIRGETNTMPLHVEILYNEYNFVAAFAVASLLALLALVTLVLKSALEWRYGYHGKG
                     SGH"
     gene            8051..9097
                     /locus_tag="VF589_06205"
     CDS             8051..9097
                     /locus_tag="VF589_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX8257206.1"
                     /translation="MSIEVEHVVKRFGAFPALNGVSLEARPGEFLALLGPSGSGKTTL
                     LRIMAGLEFPGSGSVFFDRKDVTGLAVRDRGVGFVFQHYALFRHMSVAENVAFGLRVK
                     RRGRPANAEIERRAQELLDLVQLGHLGHRLPAQLSGGQRQRVALARALAVDPTLLLLD
                     EPFGALDAKVRKDLRRWLRGLHDQMGLTSIFVTHDQEEALELADRVVVMEHGTIVQVA
                     TPQQIYDRPATPFVFDFVGESNRIPVVVRHGEVSLLGRRLAVPVESGFEGEGELFFRP
                     HDAAIVEAEAGALPLLVESVRRRGGTTRVEGMVEGCRRPIEIFTTAEAAPPPGGRVML
                     ALRSASLFPLEAAA"
     gene            9129..9329
                     /locus_tag="VF589_06210"
     CDS             9129..9329
                     /locus_tag="VF589_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257207.1"
                     /translation="MNMEWSPSAATEPAWEDIRRLLAPLGRIPAPPASGNPIRGLALG
                     MLISIPLWAGLASVAVALASGL"
     gene            9520..9753
                     /locus_tag="VF589_06215"
     CDS             9520..9753
                     /locus_tag="VF589_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257208.1"
                     /translation="MFVKWNALSEQARASEAATQPQTGGQRSPAAAAALGDRVGEMVA
                     TGNCRGGEQMALDAGDLGLARAVRDHCNAPVAQ"
     gene            complement(9758..10243)
                     /locus_tag="VF589_06220"
     CDS             complement(9758..10243)
                     /locus_tag="VF589_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8257209.1"
                     /translation="MAVLFTSDTHFGDHRTLNIHRRPFADVAAMDAALVAGWNAAVAL
                     DDEVWHLGDFARRAADVAPLLARLHGAKHLIRGNNDSPEAAAAAGWASVRDYAEIECD
                     GRRLVLCHYPFRSWNGQHRKALNLHGHSHGKLKPMPRQYDVGVDARGWRPVTLAELVG
                     G"
     gene            complement(10383..10664)
                     /locus_tag="VF589_06225"
     CDS             complement(10383..10664)
                     /locus_tag="VF589_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257210.1"
                     /translation="MKRLSIALLGPGLLALAACGGGGGEANTAETGGVDTANIQADDL
                     TLPADNSLGVLPGDPLGNEGALGAGNAADLNAVGTADFNAADLNGTNTQ"
BASE COUNT         1768 a         3780 c         3466 g         1772 t
ORIGIN      
        1 agggccgtag cggcgtgggg ctgcgacgag tgcgtcgcga cggcgttggg cgatccgtag
       61 catcgacggg aatgtccgca tttcacccat tgcggacatt cggcgagtgt ctgctttcga
      121 cccaatgtcg acattcggcc cagcatctga aagcgccgaa gcggcaatgt ccgttcatgg
      181 gtggtgagcg gacatgcaca cccgtatcat gaaaggatgg ctacggcgca tctcctgttc
      241 ggctatctag gatcaggtaa gaccaccttc gccaagcgcc tggaggtcga gcacgcggcg
      301 gttcggttca cacccgacga gtggatggcg cgcctgtttg gagacgatcc gcccgaggaa
      361 agcttcccgg agaaggcgga ggccattctg gagttgctcg aaccgctgtg gacacgctgc
      421 ctctcgctcg gtttagatgt tgtcctggat tacgggttct ggacacgcaa agagcgagac
      481 gaagtgcgcg cggttgtgga gcgactcggc gcgagtgcat gcctctatcg ccttgggtgc
      541 cccgacgatg aggctcgcag gcggatcgac gcccgaaatc aggcggatga tcgtagcctc
      601 tatatagcgc ctgcaaccta taacgcgctt tggcgtcgct tcgaacctct cgatgttgat
      661 gaagctttcg tggaagtgga taatcggacc gtccgctgag ggtcgtttcc ggccatagcc
      721 gacgtcagcg gctgagtgtc cgctgtccgg cctccgcctc cgaaagcgga ctgtcggcaa
      781 ccggccaatc gctgccattc cgcgactgtc cttcaccgct taaaacaccg ctcccgatgc
      841 caagccagat agctcggatg cggccggtgc gcgggattac ccggcaccgc cgcccgccgc
      901 gcctggttga ccagcttcca gacgccctcg acgtcgttca cctgccgtga caccagcacg
      961 tccaaatcgt ccgacaggct gatcagcccg cggtcgaaca tccaatgggc ggtgccggat
     1021 aaagccaggc cgttgcggat gtcgtcgggg ccgttggttt cgaccggctt gatgtgggcg
     1081 gcctcgactt cggcgcggcc gccgccgttg atgaatttga ggccggtgac cgcgcagcgg
     1141 ctgtcatagg cgtcgagcac gatgcggcgg aacacgcggt cgcgcacgat ccgcgagctg
     1201 taatagccga cccgctcgcg ctcggcgtcg tcgacctgga acggggcctg ctcctcgcgg
     1261 acgaggccgg gcgtgtctcg actgggctcg acataagcgg ggttctcggc gggggtgccg
     1321 gcgcggggca gcagcggcgc ctcgtcgggg atgccgagat cgaggatgcg gttgaaatcc
     1381 tcgagcgaga ccgggcggac ggcccattgc ggattgacca cgccgcgctc ggggcgcccc
     1441 tcggggccgg tgaagggaac cggacgctcg aacgacaaat agctgccagg ctcgatcagc
     1501 gcgaggtgca tggcgctttt ggtgggatcg ggcacgatgc gctcgacgcg cgcgatcgcg
     1561 aaatagcccc tgctgttccg gaccttggcg ggctcataat agaggatcca gtcgccgacg
     1621 aagcgggcgg ctcggtcgag gtacatggcc gggaactgat attgcgtctc cggatggtcc
     1681 tcgtagatcg agtccgcccg gtggatgaac acgcccctcg ccactgagcc actccccctc
     1741 gagcgccgga ggctagcagg atagacagcg ccggcaaagc agccgcttcc gcaagcagcg
     1801 aacgacgggt aagctggcct cgaccggtgc ccaccgcaac aacgccctac cccgcggcct
     1861 tgccacacac tctcaaggca ctaccgccaa caaaacacgc acccacacca ccacaaccgt
     1921 cccaaaaacc gcggaaatcc gccactccgc ccccacacaa acacccctct gaggcacttt
     1981 cgccaacatc cgcaaccgcc tcccaaccgg ggcttccaat gtaggatttc gtctgacagg
     2041 ctcctcccct gcccgccacg cggcgcccgc ccggggccgc cgccgtgaca gggcggcagg
     2101 gaggcattag ctatacgggc atgatctcgc aaaagacacg ctacgcgctc cgctcattgc
     2161 tctacctggt cgaggagggc aaaggcgcgc cggtgcagct cgcgcgtatc gccgagacgc
     2221 agcaggtgcc gcgcaaatat ctggagctga tcatgctcga attgaaaaaa gccggcatcg
     2281 tcgccagcgt gcgcggcccc gccggcggct accggctggc gcgggcgccc gaggaggtgt
     2341 cgttcggcga cgtcatccgc atcatggacg ggccgatcgc gctcgtcccc tgcgccagcg
     2401 tccatttcta ttcgacctgc ggcgactgcc acgacgaggc cacctgcgcg atccgcaaag
     2461 tgctggcgat ggtccgcgaa tcgagcgccg gcatcctcga aggcacctcg ctcgcccagg
     2521 cggccggctg ggaaaccggc ctttcagcgc cagaaggtgc ttcttcagcg atgagctgag
     2581 gctgtacacc tattccctac tagggaacta gagattaggc tgctctccaa ccaaggggag
     2641 agtcgatgcc tagcctgcct aagctgccca agctgcccaa gcttccgagc ctcaagcatg
     2701 tcgccgccgc cgcggcgctg gccgccaccg ccctcgcgcc gcccgcggcg ctcggcggcg
     2761 ccgcccaggc gcaattgctc aacgtctcct acgatcccac ccgcgaactc taccgcgaca
     2821 tcaacgccaa tttcacccgg cgctggaagg cggcgagcgg ccagaatgtg accatccgcc
     2881 agagccatgg cggctcgggc aagcaggcgc gctcggtgat cgacgggctc caggccgacg
     2941 tggtgacgct cgctttggcc tacgacatcg acgagatatc cagccgcgcc aaggcgctgc
     3001 cggccaattg gcagtcgcgg ctgcccaaca acagctcgcc ttattactcg acgattgtgt
     3061 tcctggtccg caagggcaat ccgaagaaga tcgccgactg gaacgacctc atccgcccgg
     3121 acgtcaaggt gatcacgccc aaccccaaga ccagcggcgg cgcgcgctgg aattatctcg
     3181 ccgcctgggg ctatgcgcag aagaaatacg gctcgccggc caaggcgcgc gagttcctga
     3241 cgcggatgtt caagaacgtg ccggtgctcg acagcggcgc gcgcggcgcc accaccacct
     3301 tcgtccagcg cggccagggc gacgtcctgc tcgcttggga gaatgaggcg ttcctggcgg
     3361 tcaaccagct cggccgcggc aagttcgaca tcgtcgtccc ctcgatgtcg atcctggccg
     3421 agccgccggt ggcgctcgtg gacaaggtgg tcgacaagaa aggcacccgc aaggcggccg
     3481 aggcctatct caaatatctc tacacgcccg aggcgcagga gatcatcgcc aaacattatt
     3541 accggccgcg cagcccccag gtcgccgcca aatacaaggc gcgctacccg aacgtggcgc
     3601 tgctcacgat caagaatttc ggcggctggg ggcgcgccca ggcgacgcac ttcaacgatg
     3661 gcggcctgtt cgaccagatt ttcgaagctg cgaagcgctg acctccttgg cgccctagca
     3721 gggcgggccg gaagcgaggc cggcccgcca cccaccttcc caaaagcctt ctcctgcgga
     3781 gtagaatgag atgcggttgt tcctgattgc cggtgtgtcc ctgatcgcgc ttgccgcccc
     3841 ggcgcaggcg caggagccga ccgacgagag cctatccgag gcgccgtccg gcggcaccat
     3901 cgacaccatc atccaggacg aggcgccggc ggccgcgccg gcgccgaccg gcgatccggt
     3961 gctcgaccgg ctgaacgcgc tcgaagccaa ggtgaaccag ctcgagcggc gcaacgccga
     4021 gctcgagcag caggccgagc tcaaccaggg ccggatcgag agcgtcgagg cgcgcgccgg
     4081 caagaacgtc cagttcagct gggggcccac cttcgccgac accagcggcg gcttcacctt
     4141 caagccgcgc ggcgtcgtcg aggcggatta tgccgccttc ttcgaacgcg aaggcggcta
     4201 tgatttcaac aacggcaccg gcttccgccg cgcccgcatc ggcttcgaag gcaccgcctt
     4261 caaaaagttc aactaccgga tcgaggccga tttcgccggc aacacggtca acctcaccga
     4321 cgcctatctg caatatgtgg ccagcccgca attcctgatc accgccggtc agcacaaggc
     4381 gccgttcggc ctcgaatcca acaatagcga caattacaac atcttcatgg aacgcggcat
     4441 gttcaccaac gcgttcggca atgccggcgc ggaacgccgc atcggcctct ccgccgccta
     4501 ccagaccgag acgctgaacg ccgccgccgg catcttcggc gagaatgaaa gcatcggccg
     4561 ctcctcggcc acgccggtcg acgacacgcc cgacgaaagc tggggcttca acggccgcat
     4621 cgcctgggag ccgatcttcg agcccgagaa gatcctccac ctcggcgttt ccggctacca
     4681 ccgcaccgcg ctccgctcgg gcgacgtgga ggatgcggtg cggttcaacg accgtcccaa
     4741 catccgcatc gacaacggca acatcgcgga ttccggcgtc atcaccggcg tcgaaagcct
     4801 ccgctatctc gggggcgagg ctgcggcggt gttcgggccg gtgacgctgg cgggcgaata
     4861 tggccgcgtc tgggtcgacc ggcccgaggc ggacaatctc tcgttcgacg gcttctacct
     4921 ttacggcacc tggttcatca ccggagaatc gcggccgttc aggaacggca atttcgaccg
     4981 ggtgaagccc ttcacctcgc tcggccagga cggcgtcggc gcgttggagc tggcgctgcg
     5041 ctacgacaag atcaacctcg gcgacacgcc ggtcgaagcc cggatcggca accgcgccga
     5101 gagcctgacg atggggctca actggtactt caacccctac gccaaattgc tgttcaactg
     5161 ggtccgcttc gacggcgtca acacgccgct cgatccggag agcgaccgca ccgagggcga
     5221 cgtcttcgcg acccgcctcc acctcgattt ctgatcctcg aaccggagac ttcgaaatgc
     5281 gcttgagatg cacagcactc gcactgctgc tctcggcttc cctcctcgcc ggctgctcgg
     5341 gcggggatga caaacagggc ggcgcgtcgg tggagatatt gaacgtctcc tacgatccga
     5401 cgcgcgagct ctacaaggag gtgaacgccg ccttcgcgcg ccagtggcag gggcagacgg
     5461 gccagcaggt cagcttcaac atgagccatg gcggctcggg caagcagtcg cgcgcggtga
     5521 tcgacggcct cgaagccgac gtggtgacgc tggcgctcgc ctacgacatc gacgcattgc
     5581 cgagaaggcg aagcggctgc cggccgattg gcaggcgcgg ctgccgaaca acagcgcgcc
     5641 ttatttctcg acgatcgtat tcctcgtccg caagggcaat cccaggggca tccgcggctg
     5701 gggcgacctc gtgaagcccg gcgtcaaggt catcacgccc aatccgaaga ccagcggcgg
     5761 cgcccgctgg aacttcctcg ccgcctgggg ctacggccgc aaggcgggcg gatccgacgc
     5821 cgccgccgag caatatgtga gcgggctgct cggcaacgtg ccggtgctcg acagcggcgc
     5881 gcgcggctcc accaccacct tcgtcgagcg cggcatcggc gacgtcctgc tcgcctggga
     5941 gaatgaggct ttcctcgccc aggaggagct gggccagggc aagttcgaga tcgtcgttcc
     6001 ctccatgtcg atcctggccg agccgccggt tgcggtcatc gaccagaatg ccgccaagca
     6061 cggcacccag gcggtcgcgc aggcctatct gaacttcctc tatacgcggc ccgcgcagga
     6121 gatgatcgcg cgccacttct accgtccgcg cgatccgcag gtggcggcgc agcatgcggc
     6181 gcgcttcccg cgcatcgagc tgctcgagat cgatcgcgac ttcggcggct ggaaggcggc
     6241 gcaggcgcgc tatttcgacg atgggggcgc gttcgacacg atctacgccc gcatcaaacg
     6301 gtgacccgga acatgtccgc cgccgccatg ccgatcgaac cgccgatcgc cgtctcccag
     6361 ggctacaggg aagcgcgcgg ccgccgcccg cggaaggtca ggaagtcgat catccccggc
     6421 ttcggcctga ccttcggctt caccctcgcc tatctgtgcc tgatcgtgct catccccttg
     6481 agcgccgtgt tcctgaagac ggccgggatg ggctgggccg agttcgtcaa ggtcggcctg
     6541 tcggaccgcg cgctcgccgc ctaccggttg agcttcggcg ccgccttcct ggccgccacg
     6601 gtgaacgcgc tgttcgggct caacatcgcc tggatgctgg tccgctacga cttccccggc
     6661 aagcgcgtcg tcaacgcgct cgtcgacctt cccttcgcgc tccccaccgc ggtcgcgggc
     6721 atcgcgctca ccgcccttta cgccgacacc ggctggatcg ggcagtatct ggcgccgctc
     6781 ggcatccagg tcgctttcaa gccgctcggc gtcgtcatcg ccctcatctt cgtcgggctc
     6841 cccttcgtgg tgcggtcggt cgagccggtg ctcgcggacg tcggcgtcga tgtggaggaa
     6901 gccgccgcgt cgctcggcgc caatcgcctg cagactttcc gccgcgtgct gctgccggcg
     6961 atcctccccg ccttgctgac cggcttcgcg ctcgccttcg cgcgcggcgt cggcgaatat
     7021 ggctcggtga tcttcatcgc cggcaacatg ccgttcgaat ccgagatcgc gcctttgctg
     7081 atcgtcaccc agctcgaaca atatagctat acgggcgcga ccgcgatcgc ggtggtgatg
     7141 ctggtcgtct cgttcgccat cctcctcgtc atcaacctca tccaggcctg gagccgcaga
     7201 cggagcatcg accgatgacc agccgcgccg cgagcgaaag ccgcttcgcc cgtaaactcc
     7261 tgatcggcac cgcccttggc ttccttggcc tgttcgtggt gctgccgctg ctcgccgtct
     7321 tcgtcgaagc gctgcgcgag ggcttcggcc cctccctgga gtcggtgaag gagccggacg
     7381 ccttgtccgc ggtccgcctc accctcacgg tcgcggcgat cagcgttccc ttgaacatcg
     7441 tcttcggcct cgccgccgcc tggtcgatca ccaagttcga ctttcccggc aagagcatcc
     7501 taaccaccct catcgatctg cctttctcgg tctcgccggt cgtctccggg ctgatctacg
     7561 tgctgctgtt cggcgcgcag ggctggctcg gcgactggct cgccgcgcgc gacgtgcaga
     7621 tcatcttcgc ggtgccgggg atcgtgctcg caaccgtgtt cgtgaccttc cccttcgtcg
     7681 cccgcgagct catcccgctg atggccgagc agggccgcga cgacgaggag gcggccctgt
     7741 cgctcggcgc cagcggctgg cagaccttcc gccgcgtcac cctccccaac attcgctggg
     7801 gactgctcta cggcgtgctg ctctgcaacg cccgcgcgat gggcgagttc ggcgcggtct
     7861 cggtcgtctc cggccacata cgcggcgaga ccaacacgat gccgctccac gtcgagattt
     7921 tgtacaacga atataatttc gtcgccgcct tcgcggtcgc ctcgctgctc gccctgctcg
     7981 cgctcgtcac cctggtgctc aagagcgcgc tcgagtggcg ctacggatat catgggaaag
     8041 gaagcggaca ttgagcatcg aggtcgaaca tgtcgtcaag cgcttcggcg ccttcccggc
     8101 gctgaacggc gtcagcctgg aagccaggcc gggcgaattc ctagccttgc tcggcccctc
     8161 cggctcgggc aagacgacgt tgctccggat catggcgggg ctcgaattcc ccggcagcgg
     8221 cagcgtcttc ttcgaccgta aggacgtcac cggcctcgcc gtgcgcgacc gcggcgttgg
     8281 gttcgttttc cagcattacg cccttttccg ccatatgagc gtcgccgaga atgtcgcctt
     8341 cggcctgcgc gtcaagcggc gcgggcggcc cgcgaacgcg gagatcgagc ggcgcgcgca
     8401 ggagctactc gatctggtcc agctcggcca tctcggccac cgcctgccgg cgcagctttc
     8461 gggcggccag cggcagcgcg tggccctcgc ccgcgcgctc gccgtcgatc ccactttgct
     8521 gcttctcgac gagccgttcg gcgcgttgga cgccaaagtc cgcaaggatt tgcggcgctg
     8581 gctgcgcggc ctccacgacc agatggggct gacctcgatc ttcgtcaccc acgaccagga
     8641 agaggccctg gagctggccg accgggtggt ggtgatggag catggcacga tcgtccaggt
     8701 cgccacgccc cagcagatct acgaccgtcc ggcgacgccg ttcgtgttcg acttcgtcgg
     8761 tgaatcgaac cggatcccgg tcgtcgtccg ccacggcgaa gtcagcctgc tcggccgcag
     8821 gctggcggtc ccggtcgaaa gcgggttcga gggcgaaggc gaattgttct tccgccccca
     8881 cgacgccgcc atcgtcgagg ccgaggccgg cgccttgccg ctcctcgtcg agagcgtgcg
     8941 taggcgcggc ggaaccacgc gcgtcgaagg aatggtggaa ggctgccgcc gcccgatcga
     9001 aatcttcacc accgccgaag ccgcccctcc ccccggcggc cgcgtcatgc tggcgctgcg
     9061 cagcgcctcg ctgttcccgc tcgaagccgc cgcctgacgc caggtccgtt caaccggaga
     9121 gactcgcgat gaacatggaa tggagcccat ccgctgccac tgaacctgct tgggaggaca
     9181 tccgccgcct gctcgcgccg ctcggtcgca ttcccgcgcc gccggcgtcc ggcaacccca
     9241 tcaggggcct cgctctgggg atgctgatca gcatcccgct ctgggcgggc ttggccagcg
     9301 tggcggtggc cctcgcctcg ggtctttgac ccgcgccaac tggatgcaac cccgcgggtc
     9361 accggccgtt aatcctagga aggataaagc cgaacgcgca atatgttgat aaatatgatc
     9421 ttcctcgcgc tcgcggcgcc cgctcctggg cccgccccac cgccgccgcc gcccgcggcc
     9481 cggacggctc ccctgcccgc gccgagcggc taccggtcga tgttcgtcaa atggaacgcg
     9541 ctttccgagc aggcccgcgc ctcggaggcc gccacccagc cccaaaccgg gggccagcgc
     9601 agcccggccg ccgccgccgc gctcggcgat cgggtcggcg agatggtcgc gaccggcaat
     9661 tgccgcggcg gcgagcagat ggcgctcgac gccggcgacc tcggcctcgc gcgggccgtg
     9721 cgcgaccatt gcaacgcgcc ggtcgcgcaa taaagcgcta gccgccgacg agctcggcaa
     9781 gcgtcaccgg ccgccacccg cgcgcatcga ccccgacatc atattggcgc ggcatcggct
     9841 tcagcttgcc gtggctgtgg ccgtggaggt tgagcgcctt gcgatgctgg ccgttccagc
     9901 tcctgaatgg ataatggcag agcaccagcc ggcggccgtc gcactcgatc tcggcataat
     9961 cgcggacgct cgcccagccc gccgccgcgg ccgcctcggg gctatcgtta ttccccctga
    10021 tgagatgctt ggcgccgtgc aaccgcgcca gcagcggcgc cacgtcggca gcgcggcggg
    10081 cgaagtcgcc gagatgccag acctcgtcgt cgagcgcgac cgccgcattc cagccggcga
    10141 ccagcgccgc gtccatcgcc gcgacgtcgg caaacgggcg gcgatggatg ttgagcgtgc
    10201 gatggtcgcc gaaatgggtg tcgctggtga acaagaccgc catgcccgcg ccaacgcagc
    10261 cgcggcgggg ccgttgccgc gcccgcgcga caaaagagcc ccggcgctcg cgccgaggct
    10321 cagtttcacc gcctcattct agggagtttc cgcggtgcgt gtcggggtgt tcgaaggcgg
    10381 cgttactggg tgttggtgcc gttgaggtcg gcggcgttga aatcggccgt gccgacggcg
    10441 ttgagatcgg ccgcgttgcc ggcgccgagc gcgccctcgt tgccgagcgg gtccccgggc
    10501 agcacgccca gcgaattgtc ggccggaagg gtgaggtcgt cggcctggat gttggccgtg
    10561 tcgacgccgc cggtctcggc cgtgttggcc tcgccgccgc cgccgccgca agcggccaaa
    10621 gcgagcagcc cggggccgag gagagcgatc gaaagtcgtt tcatctgtga cctctatgcg
    10681 aatcgaatgg gccgccagga tcgcagcgcg gcgggggcct tgcaatcgcc aatggacggc
    10741 ggagcggagt ttaggccttt ctcgccccgc ctcccgcgat ccggca
//
LOCUS       DASVRK010000161        18639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_91700, whole genome shotgun sequence.
ACCESSION   DASVRK010000161 DASVRK010000000
VERSION     DASVRK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 18639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18639
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91700"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..986
                     /locus_tag="VF589_06230"
     CDS             408..986
                     /locus_tag="VF589_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257211.1"
                     /translation="MLLLNTAELEVRLQAFLHAAGWTRALCGHWYFTWNAFQIQCDPK
                     SVLATIPDFDLRSTDLREGAVYRDKAARRPGTLTRRPGKADEARFAVVMEADPDAVRR
                     ARAEADVVVGEIMQKPVTLEAALRERMKEDVSGTIEVVFEADPAGDARRRTKVTRLRI
                     SAPGEPPEIDVRTETLERGLASGPAALAKQPL"
     gene            1295..4039
                     /locus_tag="VF589_06235"
     CDS             1295..4039
                     /locus_tag="VF589_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257212.1"
                     /translation="MATIIDLSTLGSAGFVLEGELEQDKAGFSVSSAGDVNGDGIEDF
                     IIGAPDAGPPFTGNYSGGAVYVLYGKTSPFDARIDLASLSGAEGFVINGADAQDKTGF
                     SVSSAGDLNGDGIDDILLGAPEYSPSINGSGKAYVIFGKTGERATIELGGVDAADEFI
                     VIGGDNSMDKLGFSVSDAGDFNGDGFDDIVIGARDADTPSINAGKAYVIFGHGGAFAD
                     IPDIGALPAGAGMVLQAERVSDEAGYSVSSAGDVNGDGIDDLLVGAPKTDIGDQSDPG
                     FVSAAGTVYVVFGKTTPADIVNLADMTAADGFALRGEFYDKIGSAVSSAGDVNGDGFD
                     DIVVGSVSVSDRGAYTGAAYVLFGKASGFGTVPPGGDTPEIDLSDPIAAADGFLIIGA
                     ATQDAAGQSVSGAGDFNGDGFDDIIVGAIGAEPSGGAYATGEAYLIFGKVGGFGTIDL
                     GNLSPAQGFIIQGADDQDRVGRSVSSGDINGDGFTDIIVGASNADVYDSAPPAGQRVD
                     VGEAYVIFGTAPTTAVTRQGSLADQTIRGGAFDDRLFGFGGDDRLDGRGGDDRLDGGT
                     GDDGLFGGAGNDVYVVRDAGDEVFENSGAGTDQVLTQVSHTLRTNAHVETLLAAPASG
                     TAAINLGGSAFDNAITGNAGANRIDGKGGTDKLTGLGGNDIFVVDDNSDLVFEAAGGG
                     NDRVLASGHYVLRAGVEVELLATLAPAGTVGLRLTGNELANTVHGNAGGNVLRGNGGA
                     DILNGFAGNDQLYGGTGKDLMTGGAGVDRFYFDTALSAATNVDGISDFLAADDAIYLD
                     RAVFGGIGADGVLAGAAFRAGTAATDASDRIIYDSDTGRIYYDADGLGGAAQTLFARV
                     DIDTPLTRVDFVAFSSPAAAPPPPADAFGKWTPAGEAALATRSMLTGLDAQWGDVQIA
                     "
     gene            complement(4195..4353)
                     /locus_tag="VF589_06240"
     CDS             complement(4195..4353)
                     /locus_tag="VF589_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257213.1"
                     /translation="MRDEMDARLWVEHGAEFSRSLSEAFAKLRIVFERLHAIEFSAPW
                     RRSNTGQA"
     gene            4480..5406
                     /locus_tag="VF589_06245"
     CDS             4480..5406
                     /locus_tag="VF589_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8257214.1"
                     /translation="MRHIPPLPAVRVFEAAARHENFTTAAAELGMTQAAVSYQIRLLE
                     ERLGVPLFVRSKRRVTLSEAGRKVAPLVSGAFDDLSEAFEAIVDADEGVLSISTTQTF
                     ATNWMAPRLGSFQVERPELAVRLKTENKLIDFAAADADVGIRSGLGDWAGLRRHFLFQ
                     FHSTPMCSPGFLEAHPLARPEDILKVPRISAGDSWWKRWLECAGVAESDPPRGSGIWL
                     DSQAIEGNAAMAGHGLAMLSPIFWRQELAAGRLVQPFPIVSLDGFSYWLVYPEHKRAQ
                     PKIRAFRDWLLRQVALQAEAGPPEVYRAPPEA"
     gene            complement(5407..6180)
                     /locus_tag="VF589_06250"
     CDS             complement(5407..6180)
                     /locus_tag="VF589_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257215.1"
                     /translation="MTSTDAAAPSGVSPPSISPTAAPGVAFNYRYAFRLPAAGVSAVQ
                     EQHAQACEKLGINRCRITGMRYRLLNESDIEAMLAFKLDPAVARQFGKLGVEAVTRAE
                     GMLIDSEISGVDAGAAIKAANLTDAELNEDLRRIEAQLAKPGLKADERQRLDYDAQQI
                     RQSIRANRDQRGERQESLATTPMVFQYGSGDLVPGFDSRSPLGTALNRGGDNFMEGIG
                     LLILLVVSLAPWALLLLLAWWIARRLRTRFGHRFAATEA"
     gene            6412..8664
                     /gene="parC"
                     /locus_tag="VF589_06255"
     CDS             6412..8664
                     /gene="parC"
                     /locus_tag="VF589_06255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX8257216.1"
                     /translation="MATIADTFDQIVDTSFDDALSQRYLVYALSTITARSLPDVRDGL
                     KPVHRRLLWAMRLLRLDPGAGYKKCARVVGDTIGKYHPHGDQSVYDAMVRLAQTFSLR
                     YPLVDGQGNFGNVDGDNAAAYRYTEARLTATAAELMAGLDEGTVDYRPTYNGEEEEPE
                     IFPGLFPNLLANGASGIAVGMATSIPPHNAAEILDAAAHLIDNPKAEDSALMDFVAGP
                     DFPTGGVIVDNGESIAAAYRTGRGSFRVRARWEIEKGSQGSWSAVVTEIPFQVAKGKL
                     IEQIAALIAEKRLPILADIRDESDERIRIVLEPRSRTVEPQMLMDSLFRLTDLEVRIP
                     LNLNVLDAERTPRVMSLKQALTAWIAHQFEVLKRRTQFRIGKIDDRVELLEGYLIAYL
                     NLDRVIEIIRTEDEPKPVMIAEFGLTDRQAEAILNMRLRSLRKLEEMQIRGERDTLGK
                     EREELAKLLDSEARQRTRLKKDLAALRDRYGPDTVLGARRTLIEEAGPAREIPLEAMI
                     EREPITVIMSQRGWIRAMRGHVDLANPEAMKFKEGDALGFIFHAQTTDKILLAASNGR
                     FYTLAADKLPGGRGFGEPVRLMVDIEGEGNIVALMAATRGKLLLASSDGRGFITSAAG
                     AVAETRKGKQVVNLRPGARLTIVRPIAEEDDYVAVIGDNRKMVVFPIAELPDMSRGQG
                     VQLQRYRDGGLAEATSFKFAGGLSWGMGGASGRTRTEADLSAWRTARGAAGRMPPIGF
                     PREKPYFARK"
     gene            8695..10464
                     /locus_tag="VF589_06260"
     CDS             8695..10464
                     /locus_tag="VF589_06260"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX8257217.1"
                     /translation="MQLASPRGRKTSPDDPAGPAGAGAPRLPMAEEIIERARQDYLDA
                     LPIAAAVICTSAAGDPYIDMANEPFRQITDWGGHQGKWVSQVPFLAGGAIGEALAEFL
                     GAEDKARQFEGSDGQAIGGRHFTVRLSRLKPTPFAGSRCLLSLIDKTAQVETEKSLRA
                     EMLRDSLTGLPNRLAFNERVEAILEDPDVREGSHAVVVVDMIRFSRINECMGAIAGDE
                     LLITFARRLFSALRAGDVLARTGGDEFGILMKVGGGLGDALHLADRVTSTLTAPFRLS
                     ELEIRVDCSIGIALLSGSVELAEEVLRNAQFALKRSKQSGRPQVYEARQAKAARRRFS
                     IETELRRAIESDDLKLAFQPLIDLPSGTVSGFEALARWDHEERGAISPEEFIPVAEES
                     GLIVSLGRWALDAATRTLVDWDREAAAELPLYVAVNLSPIQIARDNVAGAVKNALEGS
                     GLAGERLMLELTESAIIQDPGRATKVLEALKRLDAKVAMDDFGTGYTSLSYLQRLPID
                     VLKIDRSFVTNMLGDRDLVAIVRAVLSLAAALGMATTAEGIESEELARTLTELGCSHG
                     QGFFFSEPLPPDQALRYWLDRSA"
     gene            complement(10440..11609)
                     /locus_tag="VF589_06265"
     CDS             complement(10440..11609)
                     /locus_tag="VF589_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX8257218.1"
                     /translation="MRRLLHILGSDEGETRYVGGCVRDTLLGLPVSDVDLATRLLPDE
                     AMARLKRASIRVVPTGLAHGTVTAVLAGRPVEVTTLRRDVATDGRRATIAYTGDWQED
                     AARRDFTINALSADPNSGEVFDYFGGEADLHERRVRFIGDALTRIAEDHLRILRFFRF
                     HARFGRGEPDAAGLDACTARANDLMALSRERIADELLKLLALPDPAPTVALMTERGIL
                     RPVLPEIEPANLPALATLIRAEAAANVAPEALRRLAALLPPDPELAAAVAARLRFSKR
                     AAKRLESGAARGESLEAEPEPLAYRLGRDEAVDRLLLSGADPAAVRRLDNWVKPRFPL
                     TGGDLIALGLPPGPVVARTLQAVERDWVEGGFAEGEAAALSLARRHVDQALRSSQ"
     gene            complement(11645..12244)
                     /locus_tag="VF589_06270"
     CDS             complement(11645..12244)
                     /locus_tag="VF589_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX8257219.1"
                     /translation="MTLASTLREALARGHARSPQLIGGDVLDDEALGSGITPAAVLVP
                     IVDRPDPTVILTLRPETMRKHPGQVSFPGGRIDPGDDGPVAAALREAAEEIALDPAAV
                     DVIGLADRYLTITGFEVTPVVAVVPPDLPLRAQPGEVAAIFEAPLHYILDPAHQQVRT
                     VHWRGRERTYYEIEWGDRRIWGATAAMIVNLGRRLELVS"
     gene            complement(12241..12810)
                     /locus_tag="VF589_06275"
     CDS             complement(12241..12810)
                     /locus_tag="VF589_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX8257220.1"
                     /translation="MPMPETPADLSGLSLADIARLAEANKLPPVERWNPSHCGDSAMR
                     IARDGTWFHEGSPIGRPLMVRLFSTILRREPDGGFVLVTPVEKLSIEVEDAPFVAVEL
                     KSESEGRERSLAFRLNTGELVLAGPDHPLRFEPREDGPHPYLEVRRGLDALIARNVYY
                     ELAELALAEAAEPPGLWSAGAFFAFESRP"
     gene            12886..13641
                     /locus_tag="VF589_06280"
     CDS             12886..13641
                     /locus_tag="VF589_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8257221.1"
                     /translation="MEFEGPRTAIVTGGARRIGAALCRALAEDGWHLLIHCHASTAEA
                     AALARELGNAATVAADLADTAAPAAIMAALDGLPPARLLVNNASRFANDAADDFTAEG
                     WDAHQAINLRAPALLSQAFAGRAGAGGGLIVNLLDAKLAQPNPDFFSYTVSKIGLAGL
                     TELTARAFAARGIRVCGIAPSVTLVSGAQTRDNFDKVHRLNALGRGVEVGEIVAALRF
                     LIAVPTVTGEVIALDGGQRFLGLPRDVQFLEMP"
     gene            13641..14042
                     /locus_tag="VF589_06285"
     CDS             13641..14042
                     /locus_tag="VF589_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX8257222.1"
                     /translation="MTFVAKLEGLVPERLQPKTRKIVLEDYSLPVDIGFHDFEIGTPQ
                     RLFVTVEVWVDEASFPESDDAAGAWNYDFLRTEIGAIAQSRRFNLQETLVRAIYDVVA
                     ARRGVTGLRVSTRKPDIYPDCAGVGVELASF"
     gene            complement(14068..14907)
                     /locus_tag="VF589_06290"
     CDS             complement(14068..14907)
                     /locus_tag="VF589_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257223.1"
                     /translation="MEVFKFITGAALLLAAAGVPAESQVPLQSPPLSYADLADLALAA
                     PVAAHVELRRASALDREQAAGIRPGETRFYIEADIVSLIRGPGGMPARVSYLVDVPNL
                     ANGRAPKLRKKAHHIVLGSTVPGLAGELRLAAPDAQIPFTQTAADRLRALLREAARAD
                     APPAITGIGRAFHVPGAIPGESETQIFLQAADGRPVSLTILRRPGEMPRWAVALSEIV
                     DEAAAAPAPDTLLWYRLACSLPAALPPSSLAAAEGAEGPAIQADYRFVRQQLGPCVRM
                     RRR"
     gene            15164..15844
                     /locus_tag="VF589_06295"
     CDS             15164..15844
                     /locus_tag="VF589_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX8257224.1"
                     /translation="MAYADQGMSRGRITAIVIVALLHALLGYAFVTGLAYNVVKQVAE
                     DLKTFDVVEEPPPPEEEPPPPPETPLVEPPPVSPPPIVRTNVQTPPPMRTVDIAPPRV
                     FNPLPAPTRITPPPPPPPPPPPAPKVVETARAKGSLPGLIRPDDYPDAAIRSEDEGTV
                     RVRLSIGTNGRVTGCNVTSSSGSSALDTATCRILTSRARFTPAKDASGNPIADSITSP
                     PITWRLVD"
     gene            15896..16684
                     /locus_tag="VF589_06300"
     CDS             15896..16684
                     /locus_tag="VF589_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8257225.1"
                     /translation="MAAPAHGGGENPYGLMAALEQGGTIAYATFGILVIMSAASWYIL
                     FVKLFEQQKIINQGRRARTSFWQAGSLKEGAAKLEKNSAYRQIVDDGLVAQEQHNKLT
                     DPIDQHDWMLGSLARSQGAIASKLSGGLALLATVGSTAPFVGLFGTVIGIYRALIKIG
                     AAGQASIDAVAGPVGEALIMTALGLAVAVPAVMAYNWLMRRNKAVLEELARFTNDLHG
                     YMMSGGTVKPQMSAGRTGSTGAPAGTTATGSATGTTSNTTARKM"
     gene            16778..17269
                     /locus_tag="VF589_06305"
     CDS             16778..17269
                     /locus_tag="VF589_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8257226.1"
                     /translation="MAMSTGPSEDGEETPMSDINTTPLVDVMLVLLIIFLITVPVVIQ
                     TVPVSLPKVRFEPTTTKPENVSLSVRANDAGGCEVYWGLQKVDSQVLLDRAVKKLEDE
                     IERQGGVDNITEETMPEAHIRGDVNTPYRCIGGAVFTMQRAGFSRVGFISEPPPGGSV
                     VRL"
     gene            17294..17719
                     /locus_tag="VF589_06310"
     CDS             17294..17719
                     /locus_tag="VF589_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8257227.1"
                     /translation="MAMSTGAAEGEPMMDINTTPLIDVMLVLLIMFIITIPIQTHAVK
                     LDLPVDDPNQVQPPIDPVKNKITVDPAGIVFWNGAPVNAVTLRQYLDITTTMTPTPEL
                     HLQPHPNARYEVVDEVLAITKRAKVTAMGFVGNEAYANF"
     gene            17851..>18639
                     /gene="thiD"
                     /locus_tag="VF589_06315"
     CDS             17851..>18639
                     /gene="thiD"
                     /locus_tag="VF589_06315"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX8257228.1"
                     /translation="MPIFSKDAPGGPAAAPAPADGPEGANRPVPRILIVAGSDSGGGA
                     GIQADIKAVTMLGGHAMTAITAVTAQNSLAVTGVLPVPAEMVIAQIDAVAGDIGVDAV
                     KIGMIGSPETAAAVAERLTSLDASLRGGGPLPIVFDPVMVASSGSILADAATIAAFER
                     LLAAATLTTPNAPELAALSGLPVATVGGLERAAVELARRTGCAVLAKGGHLPGDLLTD
                     LLVDAAGEVIRFQSPRIDSRHTHGTGCTLASGIACGLGAGLPLAE"
BASE COUNT         2969 a         6445 c         6388 g         2837 t
ORIGIN      
        1 gccggcgccg tccgggaggg gggcaagccc cgcaggcgct gcggcggccg gggagcacgc
       61 tacccggcac attgccagca gccgcacgag cggtcatcct gcgcctgatg gtgcggattg
      121 cgatcgtggc cgttctcgcg gcgctgatat ggggcgatgg ggttggcgcg gcgccgccgc
      181 cccgcgccgg cgaggaatat cagatcacca gaagctatga gaccaagcag accggtggga
      241 cgtcgtccgg aagctcccgc ggccgagaca ctttgcttga gcgtgtcgtc ggcgtccggg
      301 aagacggcct ggagcttgaa tatgaccttt cgcgcgacgt gccgccgcag gacagaaccc
      361 cccagtggga attcccggcg cgggtcttga ggaagtcgga cggctcgatg ctgctgctca
      421 acacagcgga gctcgaggtg cggctccagg cgttcctcca cgccgccggc tggacccggg
      481 cgctttgcgg gcattggtac ttcacctgga acgcctttca gatccaatgc gatccgaagt
      541 cggtgctggc gacgatccca gacttcgatc tgcggtccac cgatcttcgc gagggagccg
      601 tttatcggga caaagcggct cgccgccccg gaaccttgac gcgccggccg ggcaaggcag
      661 acgaagcgcg ctttgccgtg gtcatggagg ccgatcccga tgccgttcgc cgcgctcgcg
      721 ccgaggccga cgtggttgtg ggcgagatca tgcagaagcc ggtgacgctc gaagccgcgc
      781 ttcgcgagcg catgaaggag gacgtctccg gcacgatcga ggtcgttttc gaggccgatc
      841 ccgccggcga cgcacggcgg cggaccaagg ttaccaggct gcggatcagc gcgccggggg
      901 agccgcccga aatcgatgtt cgtactgaga cgctggagcg gggcctggcc tccggtccgg
      961 cggcgcttgc taagcagccg ctgtagatcg tgcgctttga gattgccgac gtttcgcggt
     1021 ccacgcgacg ctgacatttc gggggctttg ggagattcga aaagcctgaa aagaccctcg
     1081 tctgcgcggg aagaaccttt tgttttcagt gcggaggttc ggcgggccca cccttccaaa
     1141 aaggggcagt gacgagcccg cagcaggatg ccgataatcc cggtgtcgcc gacgatgcat
     1201 aggacgccga tgatcccggc ggcccggagg attcgctatt gtgtacagga aaggataagt
     1261 gagtagtcct cgtggggatg gggaggacca agtcgtggcg acgatcatcg atctatcgac
     1321 cttgggatcc gctggcttcg tcctcgaggg cgagctcgag caagacaagg ccggcttcag
     1381 tgtttcctcg gccggtgacg ttaacggcga cggcatcgaa gatttcatca tcggggcgcc
     1441 cgacgcggga ccgccattca ccggcaacta tagcggcggc gcggtctatg tcctctacgg
     1501 caagacgagc ccgttcgacg cgaggatcga tctcgcctcg ctcagcgggg ccgaaggctt
     1561 cgtcatcaac ggcgccgacg ctcaggacaa gaccggcttc agcgtttcgt ccgccggcga
     1621 tctcaacggc gacggcatcg acgacatcct cctcggcgcc ccggaatatt cgccctcgat
     1681 caacggcagc ggcaaggcct atgtgatctt cggcaaaacc ggcgagcggg cgacgatcga
     1741 gctcggcggg gtggacgcgg ccgacgagtt catcgtcatc ggcggcgaca actctatgga
     1801 caagctgggc ttcagcgtct cggacgccgg cgacttcaat ggcgacggct tcgacgatat
     1861 cgtcatcggc gcccgcgatg ccgacacgcc gtccatcaat gccggcaagg cctatgtgat
     1921 cttcggtcat ggcggcgcct tcgccgatat ccccgacatc ggtgcgctgc cggcaggcgc
     1981 gggcatggtc ctccaggccg agcgggtgtc ggacgaagcc ggctacagcg tctcgtcggc
     2041 aggcgacgtc aacggcgacg gaatcgacga cctgttggtc ggtgccccga aaaccgatat
     2101 cggcgaccag tccgaccccg gtttcgtgtc cgccgccggc accgtttacg tcgtgttcgg
     2161 caagacgacc ccggccgaca tcgtcaacct tgccgacatg accgcggccg acggcttcgc
     2221 cctgcgcggc gaattctacg acaagatcgg ctccgccgtc tccagcgccg gcgatgtcaa
     2281 tggcgacggc ttcgacgata tcgtcgtcgg ctcggtctcg gtgagcgata ggggtgccta
     2341 taccggcgcg gcctacgtgc tgttcgggaa ggcgagcggg ttcgggacgg tccctccggg
     2401 cggggacacg ccggagatcg atctaagcga tccgatcgcc gccgccgacg ggtttctgat
     2461 catcggcgcc gctactcagg atgcggcggg acagagcgtc tccggcgccg gcgactttaa
     2521 cggcgacggc ttcgacgaca tcatcgtcgg cgcgatcggc gccgagcctt cgggcggcgc
     2581 ctatgccacc ggcgaggcct atctcatctt cggcaaggtg ggtggattcg gcacgatcga
     2641 tctcggcaac ctcagccccg ctcaaggctt catcattcaa ggcgccgacg atcaggaccg
     2701 ggtcggccgc agcgtctcct cgggcgatat caatggcgac ggcttcaccg acatcatcgt
     2761 cggggcttcc aacgccgacg tctacgactc ggctcctccc gccggccagc gcgtcgatgt
     2821 cggcgaggcc tatgtgattt tcggcactgc ccccaccacg gccgtgactc ggcagggctc
     2881 gctggcggac cagacgatcc gcggcggtgc gttcgacgac cggctgttcg gcttcggcgg
     2941 cgacgaccgg ctcgacgggc gcggcggcga cgaccggctc gatggcggca ctggcgatga
     3001 cggcctgttc ggcggcgccg gcaacgacgt ctatgtcgtt cgagacgccg gcgacgaggt
     3061 gttcgagaat tccggggccg gcaccgacca ggtgctgacg caggtcagcc acactttgcg
     3121 gacgaacgcc catgtcgaga ctttgctcgc ggcgccggcg tcggggaccg cggcgatcaa
     3181 tctcggcggc agcgcgttcg acaacgcgat caccggcaat gccggggcca accggatcga
     3241 cggcaagggc ggcaccgaca agctgaccgg cctcggcggc aacgacatct tcgtcgtcga
     3301 cgacaatagc gacctcgtct tcgaggctgc cggcggcggc aacgaccggg tgctggcgag
     3361 cggccattat gtgctgcggg ccggggtcga ggtcgagctg ctcgccacgc tcgccccggc
     3421 ggggacggtg gggctccggc tgaccggcaa cgagcttgcc aacaccgtcc acggcaatgc
     3481 cggcggcaac gtgctgcgcg gcaatggcgg cgccgacatc ctcaatggct ttgccggcaa
     3541 cgaccaactt tacggcggca ccggcaagga tctgatgacc ggcggcgccg gcgtcgaccg
     3601 cttctatttc gacacggcgc tcagcgctgc caccaatgtc gacggcatct ccgacttcct
     3661 ggcggccgat gacgcgatct atctcgatcg ggcggtgttc ggggggatcg gcgctgacgg
     3721 cgtcctcgcc ggcgccgcgt tccgcgccgg caccgccgcc accgacgcct ccgaccggat
     3781 catctatgac agcgacaccg gccgcatcta ttatgatgcc gacggtctcg gcggcgcagc
     3841 ccagacgctg ttcgcccggg tcgatatcga caccccgctc acccgcgtcg acttcgtggc
     3901 attctcctcg cccgccgcgg ctccccctcc tccggccgac gctttcggga agtggacccc
     3961 ggccggcgaa gcggcactgg ccacgcgctc gatgctgacc ggcctcgacg cacaatgggg
     4021 cgacgtgcag atcgcctgac gggcgctcac cgccagcagg cgcgcaggcc tgcttttgcg
     4081 accgcttgcg cggattcggg cgggaggccg gggactttga aatcagggat gagcgggcgc
     4141 cggaggggtg ggcggtccgg gcggcgcccg ctcaagcttg ttgggttcac gcggtcaggc
     4201 ctggcccgtg ttcgaccggc gccagggggc gctgaactcg atcgcgtgca gccgctcgaa
     4261 aacgatccgc agcttggcga acgcctcgct cagcgagcgg ctgaactcgg cgccatgctc
     4321 gacccacagc cgagcgtcca tctcgtcgcg cattttatcg atcctttttc cttcgggagc
     4381 cgatcctgtc atcggcacag gagggagatg gagcaaaagc ggccgtttcg ccaatgaaac
     4441 gctcgacaga ggtgatagag ctggcttatc aactgcggca tgcgccacat acctcccctg
     4501 ccggccgtgc gcgtgttcga ggccgccgcc cggcacgaga atttcaccac cgccgcggcc
     4561 gagctcggca tgacccaggc cgccgtcagc taccagatcc ggctgctgga agagcggctt
     4621 ggcgtgccgt tgttcgtccg ttccaagcgc cgggtcacgc tgagcgaggc cggccgcaag
     4681 gtcgcgccgc tggtgtcggg cgcgttcgac gatctttccg aagcgttcga ggcgatcgtc
     4741 gatgccgacg aaggcgtgct cagcatcagc accacccaga ccttcgcgac caactggatg
     4801 gcgccgcggc tcggctcgtt ccaggtcgag cggccggagc tggcggtgcg tttgaagacc
     4861 gagaacaagc tgatcgactt cgccgccgcc gatgccgatg tcggcatccg cagcgggctc
     4921 ggcgactggg cggggctgcg ccggcatttc ctgttccagt tccattcgac gccgatgtgc
     4981 agccccggct tcctcgaggc gcacccgctg gccaggcccg aggacatatt gaaggtgccg
     5041 cggatcagcg ccggcgacag ctggtggaag cgctggctgg aatgcgccgg cgtggccgag
     5101 agcgacccgc cgcgcggctc cggcatctgg ctcgattcgc aggcgatcga gggcaatgcc
     5161 gcgatggcgg ggcacggcct cgccatgctg tcgccgatct tctggcggca ggagctggcc
     5221 gccgggcggc tggtgcagcc gttcccgatc gtcagcctcg acggcttttc ctactggctg
     5281 gtctatcccg agcataagcg ggcccagccc aagatccgcg cctttcgcga ctggctgctg
     5341 cgccaggtcg cgctgcaggc ggaggcgggg ccgccggaag tgtatcgcgc cccgcctgag
     5401 gcctgatcag gcctccgtgg ctgcgaagcg gtggccgaag cgcgtccgca gcctgcgcgc
     5461 gatccaccag gcgagcagca gcagcagcgc ccagggcgcc aggctcacga ccagcaggat
     5521 cagcaggccg atcccctcca tgaaattgtc gccgccgcgg ttgagcgcgg tgccgagcgg
     5581 ggagcgcgag tcgaagcctg ggacgaggtc gcccgagcca tattggaaga ccatcggcgt
     5641 cgtcgccagc gattcctggc gctcgccgcg ctggtcgcga ttggcgcgga tcgactggcg
     5701 gatctgctgc gcgtcatagt cgagccgctg gcgctcgtcg gccttgaggc cgggcttggc
     5761 gagctgggcc tcgatccggc gcagatcctc gttgagttcg gcgtcggtga ggttggccgc
     5821 cttgatcgcg gcgccggcat ccacgccgga aatctcgctg tcgatcagca tgccttcggc
     5881 gcgggtgacc gcctcgacgc cgagcttgcc gaactgccgc gcaacggcgg ggtcgagctt
     5941 gaaggcgagc atcgcctcga tgtcgctttc gttgagcagc cggtagcgca tgccggtgat
     6001 ccggcagcgg ttgatgccga gcttttcgca ggcctgggca tgctgctcct gcacggccga
     6061 gacgccggcg gccggcagcc ggaaggcgta gcgatagttg aaggcgacgc ccggcgcggc
     6121 cgtcggggag atgctcgggg ggctgacgcc ggacggggcc gcggcatcgg tggacgtcat
     6181 tgcgtcctcc gatgcgtcga aagtgcgggt ctccgtcggt tcgagtcggg acgactgctc
     6241 ttcgcctccg ccgccgcctc cttgtccgca gccgctggcg agcaacaggg cgatcagggg
     6301 cagagagcgg cgcatcggca tcctccttat gtctgtttta tgatattaaa ttacatcggt
     6361 agacaaagca agcggctcgc gctctccgcc ggccgctgct aatccgatcc gatggccacc
     6421 atcgcagaca ctttcgacca gatcgtcgac acctccttcg acgacgcgct ctcgcaacgc
     6481 tatctcgtct acgccctctc gacgatcacc gcccgctcgc tgccggacgt tcgcgacggg
     6541 ctgaagccgg tccatcgtcg cctgctgtgg gcgatgcggc tgctgcggct cgatcccggc
     6601 gccggctaca agaaatgcgc ccgcgtcgtc ggcgacacga tcggcaaata tcacccgcat
     6661 ggcgaccagt cggtctatga cgccatggtc cgcctcgccc agaccttctc gctgcgctat
     6721 cctttggtcg acgggcaggg caatttcggc aatgtcgacg gcgacaatgc cgccgcctac
     6781 cgctacactg aggcgcggct gaccgcgacc gcggccgaat tgatggccgg cctcgacgaa
     6841 ggcacggtcg attaccgccc cacatacaat ggcgaggagg aagagcccga aatcttcccc
     6901 ggcctgttcc cgaacctgct cgccaacggc gccagcggca tcgccgtcgg catggcgacc
     6961 tcgatcccgc cgcacaatgc cgccgagatc ctcgacgccg ccgcccacct gatcgacaat
     7021 cccaaggccg aggattcggc gctgatggat ttcgtcgccg gccccgattt cccgaccggc
     7081 ggcgtcatcg tcgacaatgg cgagagcatc gccgccgcct accgcaccgg ccgcggcagc
     7141 ttccgggtgc gggcgcgctg ggagatcgag aagggcagcc agggcagctg gtcggcggtg
     7201 gtgacggaga tcccgttcca ggtggccaag ggcaagctga tcgaacagat cgccgccctg
     7261 atcgccgaga agcgcctgcc gatcctcgcc gacatacgcg acgaatcgga cgagcggatc
     7321 cggatcgtgc tcgagccgag gagccgcacg gtcgagccgc agatgttgat ggacagcctg
     7381 ttccggctga ccgatctcga agtccgtatc ccgctcaatc tcaatgtgct cgacgccgag
     7441 cggacgccgc gggtgatgag cctcaaacag gcgctgacgg cgtggatcgc ccatcagttc
     7501 gaggtgctga agcgccggac tcaattccgg atcggcaaga tcgacgaccg ggtcgagctg
     7561 ctcgaaggct atctgatcgc ctatctgaac ctcgaccggg tgatcgagat catccgcacc
     7621 gaggacgagc ccaagccggt gatgatcgcc gagttcggcc tcaccgaccg ccaggcggaa
     7681 gcgatcctca acatgcgcct ccggagcctg cgcaagctcg aggagatgca gatcaggggc
     7741 gagcgcgaca cgctcggcaa ggagcgcgag gaactcgcga aattgctcga cagcgaggcg
     7801 cgccagcgca cccggctcaa gaaggatctg gcggcgctgc gcgaccgcta cggccccgat
     7861 accgtgctcg gcgcgcggcg caccctgatc gaggaagccg gcccggcccg cgaaatcccg
     7921 ctcgaggcga tgatcgagcg cgagccgatc acggtgatca tgtcgcagcg cggttggatc
     7981 cgggcgatgc gcggccatgt cgacctcgcc aatcccgaag cgatgaagtt caaggaaggc
     8041 gacgccttgg gcttcatctt ccacgcccag accaccgaca agatcctgct cgcggcctcg
     8101 aacggccgct tctacacgct cgcggcggac aagctgccgg gcgggcgcgg cttcggcgag
     8161 ccggtgcggc tgatggtcga catcgaaggg gagggcaata tcgtcgcctt gatggcggcc
     8221 acccgcggca agctgctgct ggcctcgtcc gacgggcgcg ggttcatcac ttcggccgcc
     8281 ggcgcggtcg ccgagacgcg caagggcaag caggtggtga atttgcggcc gggcgcgcgg
     8341 ctgaccatcg tccgcccgat cgccgaggag gacgattatg tggcggtgat cggcgacaac
     8401 cggaaaatgg tcgtgttccc gatcgccgag cttcccgaca tgagccgcgg ccagggcgtc
     8461 cagctgcagc gctaccgcga cggcgggctt gccgaggcca ccagcttcaa gttcgccggc
     8521 gggctgagct ggggcatggg cggcgccagc ggccggaccc ggaccgaggc cgacctttcc
     8581 gcctggcgga cggcgcgcgg cgccgccggc cggatgccgc cgatcggctt tccccgcgag
     8641 aagccgtatt tcgcgcgcaa ataagcgtta accacttgcg cgtatggctg gcccatgcag
     8701 ctggcctcgc cccgcgggcg caagacatcg ccggacgatc ccgccggccc cgccggcgcg
     8761 ggcgcgccgc gcctgccgat ggccgaggag atcatcgagc gcgcgcgcca ggattatctc
     8821 gacgccttgc cgatcgccgc cgcggtgatt tgcaccagcg ccgccggcga cccttatatc
     8881 gacatggcca acgagccgtt ccggcagatc accgattggg gcggccatca aggcaaatgg
     8941 gtgagccagg tgccgttcct cgccggcggc gcgattggcg aggcgctggc ggaattcctc
     9001 ggcgccgagg acaaggcgcg ccagttcgaa ggcagcgacg gccaggcgat cggcggccgc
     9061 cacttcacgg tccggctgtc gcggctgaag ccgaccccgt tcgcgggcag ccgctgcctc
     9121 ctgtcgctga tcgacaagac cgcgcaggtc gagaccgaga agagcctgcg cgccgaaatg
     9181 ctgcgcgaca gcctcaccgg ccttcccaac cggctcgcct tcaacgagcg ggtcgaggcg
     9241 atcctcgaag acccggacgt ccgggagggc agccacgcgg tggtcgtcgt cgacatgatc
     9301 cgcttcagcc ggatcaacga atgcatgggc gcgatcgccg gcgacgagct tctgatcacc
     9361 ttcgcgcggc ggctgttctc ggccctgcgc gccggtgacg tgctcgcccg caccggcggc
     9421 gacgagttcg gcatcctgat gaaggtcggc ggcggcctcg gcgacgcgct gcatctcgcc
     9481 gaccgcgtca cctcgaccct gaccgcgccg ttccggctgt ccgagctcga gatccgggtc
     9541 gactgctcga tcggcatcgc gcttctgtcc ggcagcgtcg agctggccga agaggtgctg
     9601 cgcaacgcgc aattcgcgtt gaagcgttcc aagcagtcgg ggcggccgca agtctacgaa
     9661 gccaggcagg ccaaggcggc gcggcggcgg ttcagcatcg agaccgagct caggcgcgcg
     9721 atcgagagcg acgatctgaa gctcgccttc cagccgctga tcgaccttcc ctccggcacc
     9781 gtctccggct tcgaggcgct ggcgcggtgg gaccatgagg aacgcggcgc catctcgccc
     9841 gaggaattca tcccggtggc ggaagaatcg gggctgatcg tctcgctcgg ccgctgggcg
     9901 ctggacgcgg ctacccggac cctagtcgac tgggaccgcg aggcggcggc cgagctgccg
     9961 ctttacgtcg cggtcaacct ctcgccgatc cagatcgccc gcgacaatgt cgccggcgcg
    10021 gtcaagaatg cgctcgaggg cagcggcctc gccggcgagc ggctgatgct cgagctgacc
    10081 gaaagcgcta tcatccagga cccggggcgg gcgaccaagg tgctggaggc cctgaagcgg
    10141 ctcgacgcca aggtggcgat ggacgatttc gggaccggct acacctcgct ttcctacctc
    10201 cagcggctgc cgatcgacgt gctcaagatc gaccgcagct tcgtcaccaa catgctcggc
    10261 gaccgcgatt tggtcgctat cgtccgggcg gtgctgtcgt tggccgccgc gctcggcatg
    10321 gccaccaccg ccgaaggcat cgaatcggag gaactggcgc ggacgctgac cgagctcggc
    10381 tgctcgcacg gccagggctt cttcttctcc gagccgctgc cgcccgacca ggcgcttcgc
    10441 tactggctcg accgcagcgc ctgatcgacg tggcgccggg cgaggctcag cgccgccgcc
    10501 tcgccctccg caaaaccgcc ctccacccag tcgcgctcga ccgcctgcag ggtcctggcg
    10561 acgaccggcc ccggcggcag ccccaaagcg atgagatcgc cgccggtgag cgggaagcgc
    10621 ggcttcaccc aattatcgag ccgacgcacc gcggccggat ccgcacccga cagcaacagc
    10681 cggtcgacgg cctcgtcccg gcccagccgg taggcgagcg gctcgggctc cgcttccagg
    10741 ctctcgccgc gcgcggcccc gctctccagc cgtttggcgg cgcgcttgga gaagcgcagc
    10801 cgcgccgcca ccgccgccgc cagctcggga tcgggcggca gcaatgcggc cagccggcgc
    10861 agcgcttccg gcgccacgtt cgccgccgct tcggcccgaa tcagcgtcgc gagcgcgggc
    10921 aggttggcgg gctcgatctc cggcagcacc gggcggagga tgccgcgctc ggtcatcagc
    10981 gccacggtcg gcgcggggtc gggcagagcg agcagcttca ggagctcgtc ggcgatgcgc
    11041 tcgcgcgaca gcgccatcaa atcgttggcg cgggcggtgc aggcgtcgag gccggccgca
    11101 tcgggctcgc cgcggccgaa ccgggcgtgg aagcggaaga agcgcaggat gcgcagatgg
    11161 tcctcggcga tccgggtcag cgcgtcgccg atgaagcgga cccgccgctc gtggagatcg
    11221 gcctcgccgc cgaaatagtc gaacacctcg ccgctattcg ggtccgccga caaagcgttg
    11281 atggtgaagt cgcggcgggc cgcgtcctcc tgccaatccc cggtataggc gattgtcgcg
    11341 cgcctgccgt cggtggcgac gtcgcggcgc agcgtcgtca cttcgacggg gcggccggcg
    11401 agcaccgccg tcaccgtgcc gtgggcgagg ccggtcggca cgacccggat cgaagcgcgc
    11461 ttaaggcggg ccatcgcctc gtccgggagc agccgggtgg cgaggtcgac gtcgctgacc
    11521 ggcagcccga gcagggtgtc gcgcacgcag ccgccgacat agcgggtctc gccctcatcg
    11581 gacccgagga tatggagcag gcgccgcatc tccggcgcgg cctgccaatc cgcttccggc
    11641 agcctcaaga caccagctcc agccgccggc cgaggttgac gatcatcgcc gcggtcgcgc
    11701 cccagatgcg gcggtccccc cattcgatct cgtaataagt gcgctcgcgg ccgcgccagt
    11761 ggacggtgcg gacctgctgg tgggcgggat cgagtatata gtggagcggg gcctcgaaga
    11821 tcgccgccac ttctcccggc tgcgcgcgca gcggcaggtc cggagggacc accgccacca
    11881 ccggcgtcac ttcgaagccg gtgatggtga gatagcggtc ggcgaggccg atcacgtcga
    11941 ccgcggccgg atcgagcgcg atctcctcgg ccgcctcgcg cagggcggcg gcgactggcc
    12001 cgtcgtcgcc cggatcgatg cggccgccgg gaaagctcac ctgccccgga tgcttgcgca
    12061 tcgtttcggg gcggagcgtc aggatcacgg tcggatcggg ccggtcgacg atcggcacca
    12121 gcaccgccgc gggcgtgatg ccggagccca gcgcctcgtc gtcgagcacg tcgccgccga
    12181 tcagctgggg gctgcgcgca tggccccgcg ccagtgcctc gcggagagtg gacgcgaggg
    12241 tcacggccgg gactcgaagg cgaagaaggc gccggcgctc cacaggccgg gcggctcggc
    12301 ggcctcggcc agggcgagct cggccagctc gtaatagaca ttgcgggcga tcaaagcgtc
    12361 gagcccgcgc ctgacttcca gatagggatg cggcccatcc tcgcgcggct cgaagcggag
    12421 cgggtggtcg ggccccgcta gcacgagctc gccggtgttc agccggaagg ccagcgaacg
    12481 ctcgcgcccc tcgctttcgc tcttcagctc gacggcgacg aacggcgcgt cctcaacctc
    12541 gatgctgagc ttctcgaccg gcgtcaccag cacgaagccg ccgtccggct cccgccgcaa
    12601 gatcgtcgag aacagccgca ccatcagcgg ccggccgatc ggactgcctt cgtgaaacca
    12661 ggtgccgtcg cgcgcgatcc gcatcgcgct gtcgccgcag tggctcggat tccagcgctc
    12721 gaccggcggc agcttgttgg cctcggcgag ccgcgcgatg tcggcgagcg acaggccgga
    12781 aaggtcggcg ggagtttcgg gcatcggcac gaggggctag gtagaagata gcgatggcca
    12841 gcgcaaagct tgccgcgaag ggcccgcgac cctatcgggc gcccgatgga gtttgaaggc
    12901 ccccgcaccg cgatcgtgac cggcggcgcc aggcggatcg gcgcggcgct ttgccgcgcg
    12961 ctcgccgaag acggctggca tctcctcatc cactgccatg cctccaccgc cgaggcggcc
    13021 gcgctggcgc gcgagctcgg caacgccgcc accgttgccg cggatctcgc cgataccgcc
    13081 gctccggccg cgatcatggc cgcgctcgac ggcctcccgc cggcgcggct gctggtcaac
    13141 aacgcgtcgc gcttcgccaa tgacgccgcc gacgatttca cggccgaggg ctgggacgcg
    13201 caccaggcga tcaacctgcg cgcgcccgcc ttgctgtcgc aggccttcgc cggtcgggcg
    13261 ggggcgggcg gcggcctgat cgtcaacctg ctcgacgcca agctcgccca gcccaacccg
    13321 gacttcttca gctacaccgt ctcgaagatc gggcttgccg gcctcaccga gctcaccgcc
    13381 cgggctttcg cggcgcgcgg gatcagggtc tgcggcatcg cgccgtcggt gaccctggtt
    13441 tcgggcgcgc agacccgcga caatttcgac aaggtccacc gcctgaacgc gctcggccgc
    13501 ggcgtcgagg tcggcgagat cgtggccgcc ttgcgcttcc tgatcgccgt cccgaccgtc
    13561 accggcgagg tgatcgcgct cgacggtggc cagcgctttc tcggcctgcc gcgcgacgtc
    13621 caattcctgg agatgccctg atgacctttg tcgccaagct cgaaggcctg gtgccggaac
    13681 ggctgcagcc gaagacgcgc aagatcgtgc tcgaggatta cagcctgccg gtcgacatcg
    13741 gcttccacga tttcgagatc ggcacgccgc aacgcctgtt cgtcaccgtc gaagtgtggg
    13801 tcgatgaagc ctccttcccc gaaagcgacg atgcggcggg cgcgtggaat tacgatttcc
    13861 tccgcaccga gatcggcgcg atcgcgcaaa gccggcggtt caacctgcag gagaccctgg
    13921 tccgcgccat ttacgacgtc gtcgccgccc gccgcggcgt caccggcctc agggtctcga
    13981 cccgcaagcc cgacatctat ccggactgcg ccggcgtggg ggtcgagctc gcctcgttct
    14041 aggccggtcc aggcagccgg tcgcgaccta tcgccgccgc atcctgacgc aggggccgag
    14101 ctgctgccgg acgaagcggt aatccgcctg gatcgccggc ccctcggcgc cctcggcggc
    14161 ggcgaggctg gacggcggca gcgcagcggg cagcgagcag gcgagccggt accagagcaa
    14221 cgtgtccggc gccggcgcgg ccgcggcctc gtcgacgatc tcgctcagcg ccaccgccca
    14281 gcgcggcatt tcgccgggcc gccgcaagat cgtcagcgac acgggccggc cgtcggccgc
    14341 ctgcaggaag atttgggttt cgctctcgcc cggtatggcg ccgggcacgt ggaaggcgcg
    14401 gccgatgccg gtgatggcgg gcggcgcgtc ggcgcgggcc gcctcgcgca gcagcgcgcg
    14461 cagccggtcc gcggcggtct gggtgaacgg gatctgcgcg tcgggggccg ccagccggag
    14521 ctcgcccgcc aggccgggga ccgtgctccc cagcacgata tgatgcgcct tcttgcggag
    14581 cttcggggcg cggccgttgg cgaggttcgg cacatcgacc aggtagctga cccgcgccgg
    14641 catcccgccc gggcctcgaa tcagcgagac gatgtcggct tcgatataga aacgtgtctc
    14701 gccgggccgg attccggcgg cctgctcgcg gtcgagcgcg ctagcccggc gcaactcgac
    14761 atgcgccgcc accggcgccg ccagcgccag gtcggcgagg tcggcatagc tgagcggcgg
    14821 cgactggagc gggacctgcg attcggccgg gacgccggcg gcggcgagaa gaagtgccgc
    14881 acccgtaata aatttgaaca cttccatcga tcctccccgc gccgccgcac accggctaac
    14941 cacctcaatc cccatagctt agccccatta accgcggatt gcagccgctt tgcggccgtg
    15001 catgccgccg cgcgggggag gattgcgtcc ccgggagtgt cgcgatagag acacggcgcc
    15061 tgtccatgat atattaagac atggatggac ccggaacggt ttggctgcgt tgtgtgtgcg
    15121 cctgaaccgg cgaaaaaata gagagaggga gtgtggcggc ttaatggcat atgctgatca
    15181 aggaatgagc aggggccgca tcacggcgat cgtgatcgtc gcgttgcttc acgctcttct
    15241 cggctacgca ttcgtcaccg gcctggccta taatgtggtc aagcaagtgg ctgaggacct
    15301 caagacgttc gacgtggtgg aagagccgcc cccccccgag gaggagccgc cgccgccgcc
    15361 cgagactccg ctggtcgagc cgcccccggt ctcgccgccg ccgatcgtgc ggaccaacgt
    15421 ccagacgccg ccgccgatgc ggacggtcga catcgctccg ccgcgggtgt tcaacccgct
    15481 gccggcgccg acgcggatca ctccgccgcc gccgcctccg ccgccgccgc cccccgcgcc
    15541 caaggtcgtg gagacggcac gcgccaaggg cagcttgccc ggcctcatca ggccggacga
    15601 ttatcctgac gcggcgatcc ggagcgagga tgaaggcacg gtgcgagtgc gcctttcgat
    15661 cgggaccaac gggcgagtga ccggttgcaa cgtgacgagc tcgagcgggt cgtccgcgct
    15721 cgatacggcg acgtgcagga tcctgacgtc ccgggcgcgc ttcactcccg ctaaagatgc
    15781 cagcggcaat ccgatcgccg acagcatcac atccccaccg atcacttggc gactggtcga
    15841 ctgatacggc tcccattgtt gcattattgt aagatcaaga gaggaaagat tgactatggc
    15901 agcaccagca catggcggcg gtgaaaaccc ctatggcctc atggccgcac tcgaacaggg
    15961 cggcacgatc gcctacgcga ccttcgggat cctcgtgatc atgtcggccg cgtcctggta
    16021 catcctgttc gtcaagctgt tcgagcagca gaagatcatc aaccagggcc gccgcgcccg
    16081 caccagcttc tggcaggcgg gcagcctcaa ggaaggcgcg gccaagcttg agaagaacag
    16141 cgcctaccgg cagatcgtcg atgacggcct ggtcgcgcag gagcagcaca acaagctcac
    16201 cgatccgatc gaccagcatg actggatgct cggctcgctc gcccgcagcc agggcgcgat
    16261 cgcgtcgaag ctgtcgggcg gcctcgcgct gctcgccacg gtcggctcga cggcgccgtt
    16321 cgtcggcctg ttcggcacgg tgatcggcat ctaccgcgcg ctgatcaaga tcggtgccgc
    16381 cggccaggcg tcgatcgacg cggtcgccgg cccggtcggc gaggcgctga tcatgaccgc
    16441 gctcggcctg gccgtggcgg tcccggcggt gatggcctat aactggctga tgcgccgcaa
    16501 caaggcggtg ctcgaggagc tggctcgctt caccaacgac ctgcatggct atatgatgtc
    16561 gggcgggacg gtgaagccgc aaatgtcggc gggccgcacc ggttcgaccg gcgccccggc
    16621 gggcacgacc gcgaccggca gcgccaccgg taccaccagc aacaccaccg cgcgcaagat
    16681 gtaagtgcct ggggagggcg gcgccgcgtg aggcgccggc cctccccgcc atcaacgggc
    16741 gccccggcgc ccgacggtca ggacaggaat ttcaaatatg gccatgagca caggcccctc
    16801 cgaagacggc gaagaaaccc cgatgtcgga catcaacacg acgccgctgg tcgacgtcat
    16861 gctggtgctc ctgatcatct tcctcatcac cgtgccggtc gtcatccaga ccgtgccggt
    16921 aagcctgccc aaggtgaggt tcgaacccac cacgaccaag cccgagaacg tgtcgctgtc
    16981 ggtgcgcgcc aacgatgccg gcggctgcga ggtctattgg ggcctccaga aggtcgattc
    17041 ccaggttttg ctcgaccgcg cggtcaagaa gctcgaggac gagatcgaac gccaaggcgg
    17101 cgtcgacaac atcaccgagg agaccatgcc cgaggcgcat atccgcggcg acgtcaatac
    17161 gccctaccgg tgcatcggcg gcgccgtctt caccatgcag cgggccggct tcagccgggt
    17221 cgggttcatc tccgagcccc cgccgggcgg ctcggtcgtc cgcctctaat caccgcattc
    17281 aggagtaatt tacatggcaa tgtcaacggg tgcggccgaa ggcgaaccga tgatggatat
    17341 caacacgacg ccgctgatcg acgtcatgct ggtgctgctc atcatgttca tcatcaccat
    17401 ccccatccag acccacgcgg tgaagctcga tctgccggtc gacgacccca atcaggtcca
    17461 gccgccgatc gatccggtca agaacaagat cacggtcgat cccgccggaa tcgtcttctg
    17521 gaacggagcg ccggtaaacg cggtgacgct gcggcaatat ctcgacatca ccacgacgat
    17581 gacgccgacg ccggagcttc acctccagcc gcacccgaac gcccgctacg aagtggtcga
    17641 cgaagtgctg gccatcacca agcgcgccaa ggtgacggcg atgggcttcg tcggcaacga
    17701 ggcctacgcc aacttctgag cgcggccttg ggcgcaccaa ccggggggcg gcctggcggc
    17761 cgcccctttt gcgtccgggc gccggctgct tcgggtctcg atcggccggg cgcttgcgga
    17821 ccccgcgcgg cctggggcat gacgcccgcc atgccgatct tcagcaaaga cgcgcccggc
    17881 gggccggccg cggcgccggc cccagccgac ggtcccgagg gcgccaaccg tccggtgccg
    17941 cgcatcctga tcgtcgccgg ctccgattcc ggcggcggcg ccggcatcca ggccgacatc
    18001 aaggccgtga ccatgctcgg cggccacgcc atgaccgcga tcaccgcagt cacggcgcag
    18061 aacagcctcg ccgtgacggg cgtgctgccg gtgccggccg aaatggtcat cgcgcagatc
    18121 gacgcggtgg ccggggatat cggtgtcgac gccgtcaaga tcggcatgat cggatcgccg
    18181 gagaccgccg cggccgtggc ggaacggctc acctccctcg acgcgtcgct gcgcggcggc
    18241 ggcccgctcc cgatcgtctt cgatccggtg atggtggcga gcagcggctc catccttgcg
    18301 gatgccgcga ccatcgccgc cttcgagcgg ctgctggcgg cggcgacgct gactaccccc
    18361 aacgctcccg agctcgccgc tttgtcgggc ctgccggtgg cgacggtcgg cggcctcgag
    18421 cgggcggcgg tggagctcgc ccgccgcacc ggctgcgccg tgctggccaa gggcggccac
    18481 cttcccggcg acctgctgac cgatcttctc gtcgacgcag ccggcgaagt gatccggttc
    18541 cagagcccgc gcatcgacag ccgccacact cacggcaccg gctgcacgct ggcgagcggc
    18601 atcgcctgcg gcctcggcgc ggggctgccg ctggccgag
//
LOCUS       DASVRK010000162         4883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_403990, whole genome shotgun sequence.
ACCESSION   DASVRK010000162 DASVRK010000000
VERSION     DASVRK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403990"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1194
                     /locus_tag="VF589_06320"
     CDS             91..1194
                     /locus_tag="VF589_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8257229.1"
                     /translation="MVEAVARPAPKPNSQARWPEAIALSVALWLFVLLLFLPIIVAKY
                     EGENWTSVALDCSTIPVSILLAMPMFAVFRNTVQYALKHRVIVMIVTVIFTAVVNTAY
                     DITFQTIVSANFDGTFESLGTDFSRAYPTLLNYILVFGVNMALFQVSFARRAALKQEL
                     QLSQALANAQQAQLAALRYQLNPHFLFNALNSISALIVTKRNEDAEQMTDKLSSFLRN
                     SLTTDPAELVPLEEELALIEEYLDIEGVRFGDRLNVEIACDPDAGVALVPGFLVQPLV
                     ENAIKHGVAPSREPVNISVSATVEEGDLCIRVENDSVPGGNQIQSARSGVGLVNVKRR
                     LEAVYGKSASLTAERTERGYTATICIPEIQRAS"
     gene            1246..2010
                     /locus_tag="VF589_06325"
     CDS             1246..2010
                     /locus_tag="VF589_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8257230.1"
                     /translation="MRVLLVDDEALALDRLRTFFGDIEDVEVVGQAQDGDEALAEIER
                     LRPDLVILDIQMPGRNGLRAAAELNVEPRPELVFVTAHEHYAPDAFDVDAADYLLKPV
                     RFDRLRQAVERARRRRSMRDQAARAGQLEEEVAALRSHGAVQHDDAGFWVPERDGQRR
                     VPIETIDWIEAARDYVLLHTSVRSHLLRTTMSALEEKLAGGPLLRVHRSAFVRPDKVA
                     EVKRANRSLCLVLADGAQVQVGPSYVGAVKDALRLE"
     gene            complement(2014..2184)
                     /locus_tag="VF589_06330"
     CDS             complement(2014..2184)
                     /locus_tag="VF589_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257231.1"
                     /translation="MAGTSIFGEPIATPARSRDQAGPTRTYPYQGLFGMKLGGPRMGG
                     NPRAERMTASLG"
     gene            2586..3446
                     /locus_tag="VF589_06335"
     CDS             2586..3446
                     /locus_tag="VF589_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8257232.1"
                     /translation="MGRWRRWIGIAAAMAAALVVLAGGAVFIGSEYVMGRGHPPFRER
                     LAAPTAAQAADAPRQARLLGCLSCHGEGLKGNVMVDIPNVAWIQAPNLTEVAARTGDQ
                     QLAAAIRQGIGHDRRPLFVMPSAMYSRLSDGEVAALIGWIRSLPRVPGRIEGFRARPL
                     GRVALVAGKLRPATAQMEEFRTAAPLDAGGGHAAGRRIASVTCAECHGPALSGRVMET
                     GDVAPDLAIAGAYDFEQFKTLLRTGRPPSGRKLGLMAEVARQDFVHLTEAELRALHGY
                     LAARAAKLPD"
     gene            complement(3450..4496)
                     /locus_tag="VF589_06340"
     CDS             complement(3450..4496)
                     /locus_tag="VF589_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257233.1"
                     /translation="MRHPLIAAALAVLALSGAIAAQSGADGSDSGEIDWRIEAGDRAA
                     PGMVQFTLSRSLPGGGQWIHSNTRSLAELQGLAPAQLASAQGEQVRFRLVREAGSFDC
                     GGIARRQRGTGECRFAADPAFVQALARRRIGAASPRQLLSLALARIGPAYIDELGRQR
                     YARPTVADLVRAGDHGADLDYLREMGALGYRAGTLAGLIRMRDHGVTPEFAGELVRLG
                     VRDLGPDALVRLRDHGVSPDFVADLRRLGYGRLAIEELVRLRDHGVSAGYVGALREHG
                     YNLPTGEIIRLRDHGVSADFVGELRGLGYGRLSADEIVRLRDHGVSADFIRRANAGGR
                     RSVDELVRLRNDGA"
     gene            complement(4665..4805)
                     /locus_tag="VF589_06345"
     CDS             complement(4665..4805)
                     /locus_tag="VF589_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257234.1"
                     /translation="MHIYQKMKRLRLLLIAAAAFGCSIATLSGTLAAFHAAPAAMAGP
                     FA"
BASE COUNT          762 a         1642 c         1720 g          759 t
ORIGIN      
        1 catgctggcg aaatggcttc gagtgcgctg attccaacca gttttcccgc cgaccgtgac
       61 tgccccccgg ttttgcggca gaagttggag gtggtcgagg ccgttgcccg tccggcgccc
      121 aagccgaaca gccaggcgcg ctggcccgag gcgatcgcgc tcagcgtggc tttgtggctg
      181 ttcgtgctgc tcctgttcct gcccatcatc gtcgccaaat atgaaggcga gaactggacc
      241 agcgtcgcgc tcgactgctc caccatcccg gtgtcgatcc tgctcgccat gccgatgttc
      301 gcggtgttcc ggaacaccgt ccaatatgcg ctgaagcacc gcgtcatcgt gatgatcgtg
      361 acggtgatct tcaccgcggt ggtgaacacc gcctacgaca tcactttcca gacgatcgtc
      421 agcgccaatt tcgacggcac gttcgagtcg ctcggcaccg atttcagccg cgcttatccc
      481 accctcctca actacatcct ggtattcggc gtgaacatgg cgctgttcca ggtgagcttc
      541 gcccgccgcg ccgcgctcaa gcaggagctg cagctctccc aggcgctggc caatgcccag
      601 caggcgcagc tcgccgcctt gcgctatcag ctcaacccgc acttcctgtt caacgcgctg
      661 aactcgatct cggcgctgat cgtcaccaag cgcaacgaag atgccgagca gatgaccgac
      721 aaattgtcga gcttcctcag aaattcgctg accaccgatc cggccgagct ggtgccgctc
      781 gaggaggagc tggcgctgat cgaggaatat ctcgacatcg aaggcgtccg cttcggcgac
      841 cggttgaatg tggagatcgc ctgcgatccg gatgctggtg tggcattggt ccccggtttc
      901 ctggtccagc cgctggtcga gaatgcgatc aagcatggcg tcgcgccgtc gcgcgagccg
      961 gtcaacatct cggtcagcgc caccgtcgag gaaggcgatt tgtgtatccg ggtcgagaat
     1021 gacagcgtcc ccggtggcaa ccagatccag tcggcgcgca gcggcgtcgg gctggtcaac
     1081 gtcaagcgcc ggctggaagc cgtctacggc aagtcggcca gcctcaccgc cgagcgtacc
     1141 gagcgcggct atacggcgac gatttgcatc cccgagatac agcgagcaag ttgactcggc
     1201 cttatactct ccggatatag attaagggct ggggaggatt gagtaatgcg tgtgctgctt
     1261 gtcgacgacg aggccctggc cctcgatcgc ctgcgcacct tcttcgggga catcgaggat
     1321 gtcgaagtgg tcggccaggc gcaggacggc gacgaggcgc tggccgagat cgagcggctg
     1381 cggcccgacc tcgtgatcct cgacatccag atgcccggcc gcaacggcct ccgtgccgcc
     1441 gccgagctca acgtcgagcc gcgcccggag ttggtgttcg tcaccgccca cgaacattat
     1501 gcccccgacg ccttcgacgt cgacgccgcc gattatctgc tgaagccggt ccggttcgac
     1561 cggctgcgcc aagcggtcga acgcgcccgc cggcgccggt cgatgcgcga ccaggccgcg
     1621 cgcgccggcc agctcgagga ggaagtggcg gcgctgcgct cgcacggagc ggtccagcat
     1681 gacgatgccg gcttctgggt gcccgaacgc gacggccagc gccgcgtgcc gatcgaaacg
     1741 atcgactgga tcgaggcggc gcgcgattat gtgctgctcc acaccagcgt ccgcagccat
     1801 ttgctgcgca ccaccatgtc ggcgctcgag gagaaactgg cgggcgggcc gctgctgcgc
     1861 gtccaccgct cggccttcgt gcggccggac aaggtcgccg aggtgaagcg ggccaaccgc
     1921 tcgttatgcc tggtgctcgc cgacggcgcg caggtgcagg tcgggccgag ctatgtcggc
     1981 gcggtcaagg acgcgctccg gctcgaatag tccttagccg aggctggccg tcatccgctc
     2041 cgcgcgagga ttgccgccca tgcggggccc gccgagcttc attccgaaca gcccctgata
     2101 gggataggtc cgggtcgggc cggcctggtc ccgcgaccgg gccggcgttg cgatcggctc
     2161 gccgaagatg gacgtcccgg ccatggctca gagcatgccc ggcagggacg tcgcggccgt
     2221 gagcagcatg gtgctgacga aaatcgccgc tacgcacgtc gacacggtcc ggaaaatctc
     2281 cgcagtcatg gcctgtctcc cttgaaccga ctccgttcgg gccggtgccg actcccctat
     2341 gcccctgagc cgtgagtcgc cgctgccctt cgacggccac gcgaatcggt tcgacaagca
     2401 tgcagcggcg cgacgaagcc gacatcgcgc gcgatgaaaa tgttactgaa atgtgaaagc
     2461 ccgcgcggcc gccgggcccg gggctgcaaa tccgtcgcgc cagcgtcggc cttcgcagca
     2521 tcggcattgc ccggcccgcg cgaagggaca gactttccga gggaacttcc attgggggat
     2581 ggggcatggg ccgctggcgg cgctggatag ggatcgcggc cgcgatggcg gcggctttgg
     2641 tggtgctcgc cggaggcgcc gtcttcatcg gctcggaata tgtgatgggg cgcggccatc
     2701 cccccttccg cgagcggctc gccgcgccca cggccgccca ggccgcggac gcgccgcgcc
     2761 aggcgcggct gctcggctgc ctcagctgcc acggcgaagg cctcaagggt aacgtcatgg
     2821 tcgacattcc caacgtcgcc tggatccagg cgcccaacct taccgaggtg gcggcgcgga
     2881 ccggcgacca gcagctcgcc gccgcgatcc gccaggggat cggccatgat cgccggcctt
     2941 tgttcgtgat gccgtccgcc atgtattcgc ggctcagcga cggcgaagtg gcggcgctga
     3001 tcggatggat acggagcctg ccgcgggtcc cgggccggat cgagggcttc cgcgcgcggc
     3061 cgctcggccg ggtcgctttg gtggccggca agctgcggcc ggcgaccgcc cagatggagg
     3121 aattccgcac cgccgccccg ctcgatgccg gcggggggca tgccgccggc cgcaggatcg
     3181 cttcggtcac ctgcgccgaa tgccacgggc cggcgctgtc gggcagggtg atggagacgg
     3241 gcgacgtggc gccggacctc gccatcgccg gcgcctatga tttcgagcag ttcaagacct
     3301 tgctgcgcac cggccgcccg ccttcgggcc gaaagctggg gctgatggcc gaagttgcgc
     3361 ggcaggattt cgttcacctc accgaggccg aactcagggc gctgcacggc tacctcgccg
     3421 cgcgagcggc gaagctgccc gactgaccct caggcgccgt cgttgcgcag cctcaccagc
     3481 tcgtcgaccg agcggcggcc gccggcattg gcgcgtcgga tgaagtcggc gctgaccccg
     3541 tggtcgcgca ggcggacgat ctcgtcggcg ctgagccggc cgtagccgag gccgcgtagc
     3601 tcgccgacga aatcggcgga aacgccgtgg tcgcgaagcc gaatgatctc gccggtgggg
     3661 agattgtagc catgctcgcg aagcgcgccg acatagccgg cggagacgcc gtggtcgcgc
     3721 agccgcacca gttcctcgat cgccaggcgg ccgtagccga gccggcgcag atcggcgacg
     3781 aaatcagggc tgacgccgtg gtcgcggagc cggacgagcg cgtcgggccc gaggtcgcgg
     3841 acgccgaggc ggaccagctc gccggcaaat tcgggggtga cgccatggtc gcgcatgcgg
     3901 atcaggccgg ccagggtgcc ggcgcgatag ccgagcgccc ccatttcgcg cagataatcg
     3961 aggtcggcgc catgatcgcc ggcgcgcacc aggtcggcga cggtcgggcg ggcgtaacgc
     4021 tggcggccga gctcgtcgat ataagcggga ccgatcctcg ccagcgccag cgagaggagc
     4081 tggcgtggcg aggcggcgcc gatccggcgg cgcgcgagcg cctggacgaa agcgggatcg
     4141 gcggcgaagc ggcactcgcc ggtgccgcgc tggcggcgcg caatgccgcc gcaatcgaaa
     4201 gaaccggcct cgcggacgag gcggaagcgg acctgctcgc cttgggccga cgcgagctgc
     4261 gccggcgcga gcccctggag ttcggcgagc gagcgggtgt tgctgtggat ccactggccg
     4321 ccgccgggga ggctgcggga gagggtgaac tgcaccatcc ctggggccgc tctgtcaccg
     4381 gcttcgatcc gccagtcgat ttcgcccgaa tccgatccgt ccgcaccgga ttgggccgcg
     4441 atggcgccgg agagcgcgag cacggccaga gcggccgcga tgagggggtg acgcatggaa
     4501 tctccattgc aaggcaaagg cgtttcgcct tccgatgcaa gcgccgggcc aaaccatccc
     4561 ctctggggcg ggcggcggct ggagccgggc gcggcggacc ggaccgtccg gtttcggacg
     4621 aagctgtcct tttccggacg gtcccgcgcg ccgccggacc gaggtcaggc gaacgggccc
     4681 gccatggcgg ccggggcggc gtggaaggcg gcgagggtgc cgctgagcgt cgcgatgctg
     4741 cagccgaacg cggcggcggc aatcagcagc aggcgaagcc gcttcatctt ctggtagata
     4801 tgcatgttcc atttcctttg ttcgtgccgc cggagggcgg cgccacggcc gctctgcccg
     4861 ggaggcgtga gagcgagcgt ggg
//
LOCUS       DASVRK010000163         8513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_31133, whole genome shotgun sequence.
ACCESSION   DASVRK010000163 DASVRK010000000
VERSION     DASVRK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31133"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..882
                     /locus_tag="VF589_06350"
     CDS             139..882
                     /locus_tag="VF589_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8257235.1"
                     /translation="MAGHSKFKNIMHRKGAQDKKRSAMFSKLSREITVAAKMGTPDPD
                     MNPRLRAAVLAAKAQSMPKDNIQRAIDKSSMGDAENYEEIRYEGFGPGGVSLIVEALT
                     DNRNRTATNVRTIFSKNGGNLGASGSVSHGFSRLGLITYPAKAGSADAVFEAAIEAGA
                     EDVESTEEGHEIWTAQEDLHEVAKALEQALGEPESTKLAWRPQTPVEVGEQEAGSLIK
                     LIDALDEDDDVQTVWGNYEVPDEVMERLS"
     gene            879..1376
                     /gene="ruvC"
                     /locus_tag="VF589_06355"
     CDS             879..1376
                     /gene="ruvC"
                     /locus_tag="VF589_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX8257236.1"
                     /translation="MIILGLDPGLGCTGWGLIAADGNRLGHVANGQIRTDAKAPLPER
                     LTALYDALCAVVADHRPGGAAVEEVFVNENPQSTLKLGQARGVILLAAARSGVAIGEY
                     APRLVKKAVVGTGAADKAQVHAMVARLLPGVKIAGPDAADALAVAITHAHHLASARAL
                     RGHAA"
     gene            1373..1966
                     /gene="ruvA"
                     /locus_tag="VF589_06360"
     CDS             1373..1966
                     /gene="ruvA"
                     /locus_tag="VF589_06360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX8257237.1"
                     /translation="MIAKLRGLLDSFGADHAVIDVQGVGYWVSASTRTLSALGAVGDE
                     IMLHTEMQVSDDAIRLIGFATAAERDWFRLLTSVQGVGARVALAILSALTDDELHRAI
                     ASGDKAMVARAQGVGPKLALRIVTELKDKAGGLVLGPAGPVPVAAGNAADAVSALLNL
                     GFKPAEASGAVAKAEAELGPGATLDALVRLALKKAAR"
     gene            complement(2087..3214)
                     /locus_tag="VF589_06365"
     CDS             complement(2087..3214)
                     /locus_tag="VF589_06365"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX8257238.1"
                     /translation="MDRREFLLAAGAAGAALGLPMRASAADPAPRPRKARSIYFDALG
                     AVDGFEEKDGKPVPTQRLIDAIRQRRIDLISMTIADVGNGPDRFQNAAQAVAMWDGMI
                     AGQPALVAKIESVADIRAARAAGKLGLIYNFQDTTALEGDAARVDTFANLGVKILQLT
                     YNKRNLAGDGCLERSNAGLSDFGREVIGKINAANVLLDLSHAGQRTIAEGIAEAKAPP
                     AITHSGCRALVDFPRNTYDAEMRALANKGGVFGVYLMPFLRPKGQATAEDLLRHLDHA
                     VNVCGEDHVGIGTDNPLMGYTIDDEARKQQREFFEERRKRGIAAPGEAPDVFNLVEGY
                     NDVFRYDRIAADLKRRGWSSARVDKVLGDNFVRLFGEVWKA"
     gene            3352..3507
                     /locus_tag="VF589_06370"
     CDS             3352..3507
                     /locus_tag="VF589_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX8257239.1"
                     /translation="MQVTAILTPAEEGGYVAFNPETGTTTQGESVEEALANLREAVEL
                     YREEFSS"
     gene            3520..3708
                     /locus_tag="VF589_06375"
     CDS             3520..3708
                     /locus_tag="VF589_06375"
                     /inference="COORDINATES: protein motif:HMM:NF036629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HEX8257240.1"
                     /translation="MVDMSILVGSVRRFGLVGPPYEVLGPSLPSEAGEPQMRILLIES
                     GEEVDYPVSEVVNDPVDA"
     gene            3708..3986
                     /locus_tag="VF589_06380"
     CDS             3708..3986
                     /locus_tag="VF589_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEX8257241.1"
                     /translation="MFAIGFDLLWHQTRQTHPKGVSQAYADIGAVLNRYGFSRVQGSV
                     YVSHGDSLVNVTLAMNALKALPWFPNCVRDIRAFKVEHWSDFTDFMKT"
     gene            3997..5025
                     /gene="ruvB"
                     /locus_tag="VF589_06385"
     CDS             3997..5025
                     /gene="ruvB"
                     /locus_tag="VF589_06385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX8257242.1"
                     /translation="MTDAARLTTPDRRPEDLDAALRPKTLDEFVGQRAARENLRVFID
                     AARHRGDALDHVLFFGPPGLGKTTLAQIVARELGVGFRATSGPVIAKSGDLAALLTNL
                     EDGDVLFIDEIHRLNPAVEEVLYPAMEDRALDLMIGEGPSARSVRIDLPKFTLVGATT
                     RQGLLTTPLRDRFGIPVRLNFYSVEELEQVVRRGARLMGLDLTEDGATEVARRSRGTP
                     RIAGRLLRRVRDFAHAAGELRVDARVADAALTRMEVDALGLDAMDRRYLTMIADIYKG
                     GPVGVETLAAGLSEPRDTIEEVIEPFLIQLGLVARTARGRCLNGRAWTHLGIVPPKGA
                     QDGLFDSN"
     gene            5147..5566
                     /locus_tag="VF589_06390"
     CDS             5147..5566
                     /locus_tag="VF589_06390"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX8257243.1"
                     /translation="MGKTHRFALRVYFEDTDTAGIVYYANYLRFMERARSDMLRAVGI
                     DQRAALEGGDGVYAVAEAYIKYRASARLDDALVVVSHVREVRAASCVIQQQVMRGSEI
                     LTHAKVTAAFLSPDGRPRRQPRAWVEIFERLKGELEA"
     gene            5572..6261
                     /gene="tolQ"
                     /locus_tag="VF589_06395"
     CDS             5572..6261
                     /gene="tolQ"
                     /locus_tag="VF589_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX8257244.1"
                     /translation="MGLTGGASEMMSPVALFLQADIVVKAVMIGLILASIWTWAIILS
                     HWLRVRRVGRENDKFEREFWKAEDIDRFNDARGGDDWPSAKVFAAGIAEWRRSTAGRN
                     VDKEGTRSRLATAMHAAVGAEVDRLADRLNVLATIGSVAPFVGLFGTVWGIMRSFSDI
                     AGANNTSLAVVAPGIAEALFATALGLFAAIPAVIAYNRMTHGINRIEAKLSRFADGFH
                     GTLSRELEAEA"
     gene            6404..6844
                     /gene="tolR"
                     /locus_tag="VF589_06400"
     CDS             6404..6844
                     /gene="tolR"
                     /locus_tag="VF589_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX8257245.1"
                     /translation="MAMGPIHSGGQRRRRTPMAEINVTPMVDVMLVLLIIFMVTAPLL
                     VAGVPVDLPETKAGALDQEQKPVQISLEASGSIFIDDAKVTEAELGQRLAQIAGASVE
                     EGGPRIFLRADRGLDYGRVMRVMGEINAAGLRRVALVSTQGQEG"
     gene            6844..7707
                     /locus_tag="VF589_06405"
     CDS             6844..7707
                     /locus_tag="VF589_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEX8257246.1"
                     /translation="MDRAEATGLGAATLGHAALLAALTLGFANVVRPPVLNDPMEVEF
                     VDEVGLESAAPVPSPAEAAPRLAEVEGPVEPVAPPPTPPPPAPVPEPLPQPKAIKPAP
                     APAPKAVERPKPPKPAPAKAKAAPAKAPPQKAAPAKAAPPKQDSAAKAKSQPKGRLSG
                     ILSGISDRETTSRSTAPPAQKAGPAVQASLVAAVRRQVKPHWKAPTGADVELLRTELS
                     IRLAKDGRVTDIDVLRTTGQNASNRPQVKLHQEQAVKAVRLASPFNLPAEYYDAWKHL
                     SPFGFDKRLSQ"
     gene            7704..>8513
                     /gene="tolB"
                     /locus_tag="VF589_06410"
     CDS             7704..>8513
                     /gene="tolB"
                     /locus_tag="VF589_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182966.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEX8257247.1"
                     /translation="MTRMKLLLGGLAATFLAALQPAHAQLAVDVTDESGNDMIIAVPA
                     MPTSQSAPTPAGTTDALGRQIAEVVVADLRGSGLFQPRGPGEVRAIGYPEVTAPAYDY
                     WPGTGAVALVQGFVRANGDGKITVGCYLYDVGLRSELTRQGYVIEPREWRRAAHKCAD
                     AIYSRLSGESPFFDSRIAYIAESGPKARRVKRLAVMDSDGANHRFLTNGQSIVLTPRF
                     SPDYRHIVYMSYSERRPSIYIYDTLTDRQRVVTESANQAFAPRFSPDGRYIL"
BASE COUNT         1408 a         2878 c         2901 g         1326 t
ORIGIN      
        1 ccggcaagag gccgacgccc tgctcgagac ttatcgcgcc gcgctcggcc tcgactgatc
       61 ggcgtcgctt gattgatggc cgccggacag gcggctaagg agttccggac agcagctttc
      121 gaagggcgga tgacggcaat ggccggccac agcaaattca agaacatcat gcatcgcaag
      181 ggcgcgcagg acaaaaagcg ctcggcgatg ttttccaaat tgagccgcga gatcacggtc
      241 gcggccaaga tgggcacgcc cgatccggac atgaacccgc ggctccgcgc cgccgtcctc
      301 gcggccaagg cgcagtcgat gccgaaggac aatatccagc gcgcgatcga caaatcctcg
      361 atgggcgatg ccgagaatta cgaggagata cgctatgagg ggttcggccc cggcggcgtg
      421 tcgctgatcg tcgaggcgct gaccgacaac cgcaaccgca ccgccaccaa cgtgcgcacc
      481 atcttctcga agaatggcgg caacctgggc gcgtcgggct cggtgagcca tggcttcagc
      541 cggctcggcc tcatcaccta cccggccaag gcgggaagcg ccgacgccgt tttcgaagcg
      601 gccatcgaag ccggcgccga ggatgtggaa tccacggagg aggggcacga aatctggacc
      661 gcgcaggagg atctgcacga agtcgccaag gcgctcgaac aggcgctcgg cgagcccgaa
      721 agcaccaaac tcgcctggcg gccgcagacg ccggtcgagg tcggcgagca ggaagccggc
      781 tcgctgatca agctgatcga tgcgctcgac gaggatgacg acgtccagac cgtgtggggc
      841 aattacgagg ttccggacga ggtgatggag cggctttcct gatcatcctc ggcctcgatc
      901 ccggcctcgg ctgcaccggc tggggtctga tcgccgccga cgggaaccgg ctcggccacg
      961 tcgccaacgg ccagatcagg accgacgcca aggcgccgct gccggagcgg ctgaccgccc
     1021 tttacgacgc gctgtgcgcc gttgtcgccg atcaccggcc gggcggcgcc gcggtcgagg
     1081 aagtgttcgt caacgaaaac ccgcaatcga ccctgaagct ggggcaggcg cggggcgtga
     1141 ttctgctcgc cgccgcgcgc agcggcgtcg ccatcggcga atatgcgccg cggctggtca
     1201 agaaggcggt ggtcggcacc ggcgccgccg acaaggcgca ggtccacgcg atggtggcgc
     1261 ggctgctgcc cggggtgaag atcgccggcc ccgatgccgc cgacgcgctg gcggtggcga
     1321 tcacccacgc ccatcatctg gcgagcgccc gcgccttgcg ggggcacgcc gcatgatcgc
     1381 gaagcttcgc ggcctgctcg acagcttcgg cgccgatcac gcggtgatcg acgtccaggg
     1441 cgtcggctat tgggtctcgg cctcgacccg caccctgtcc gcgctcggcg cggtcggcga
     1501 cgagatcatg ctccacaccg aaatgcaggt ttcggacgac gcgatccgcc tgatcggctt
     1561 cgccacggct gccgagcgcg actggttcag gttgcttacg tccgtccagg gggtgggcgc
     1621 gcgggtggcg ctggcgattc tgtcggcgct gaccgacgac gaactccacc gcgccatcgc
     1681 cagcggcgac aaggcgatgg tggcgcgcgc gcagggcgtc gggccaaagc tcgccttgcg
     1741 catcgtcacc gagctcaagg acaaggccgg cggcctcgtg ctcggcccgg cagggccggt
     1801 gccggtcgcg gccggcaatg cggcggacgc ggtctcggcc ttgctcaacc ttggcttcaa
     1861 gccggccgaa gcctcgggcg cggtggcgaa ggcggaagcg gagctcggcc cgggcgcgac
     1921 cctcgacgcg ctggtccggc tcgcgctcaa gaaagcggcg cgctagcacc ccgtcatcct
     1981 cgcgaaagcc ccccgtcatc ctggcgaaag ccaggatctc acttccttgg ggtgccggag
     2041 gaagaaaagg gagattccag cttccgctgg aatggacggt atgcgcctat gccttccaca
     2101 cctcgccgaa tagccggacg aaattgtcgc cgagcacctt gtcgacccgc gccgagctcc
     2161 agccgcggcg cttgaggtcg gcggcgatgc ggtcgtagcg gaagacgtcg ttataccctt
     2221 cgaccaggtt gaacacgtcg ggcgcctcgc cgggggcggc gatgcccctc ttgcggcgct
     2281 cctcgaaaaa ttcgcgctgc tgcttccggg cctcgtcgtc gatcgtatag cccatcagcg
     2341 gattgtcggt gccgatgccg acatggtcct cgccgcacac gttgacggca tgatcgagat
     2401 ggcggagcag gtcttccgcc gtcgcctggc ccttcggccg caggaacggc atcagataga
     2461 ctccgaacac gccgcccttg ttggccagcg cccgcatctc ggcatcgtaa gtgttgcgcg
     2521 ggaaatcgac cagcgcgcgg cagccgctat gggtgatggc gggcggcgcc ttggcttcgg
     2581 caatgccttc ggcgatggtg cgctggccgg catggctgag gtcgagcagg acattggccg
     2641 cgttgatttt gccgatcacc tcgcggccga aatccgacag cccggcattg gagcgctcga
     2701 ggcagccgtc gccggcgagg ttgcgcttgt tgtaggtgag ctggagtatc ttgacgccga
     2761 gattggcgaa ggtgtcgacc ctcgccgcat cgccttcgag agcggtcgtg tcctggaaat
     2821 tatagatgag gccgagcttg ccggcggcgc gcgccgcgcg gatgtccgcg acactctcga
     2881 tcttcgccac cagcgccggc tgaccggcga tcatgccgtc ccacatcgcc accgcctggg
     2941 cggcgttttg gaagcggtcg ggcccgttgc cgacatcggc tatcgtcatg ctgatgaggt
     3001 cgatgcgccg ctgccggatc gcgtcgatca ggcgctgcgt cggcacaggt ttgccgtcct
     3061 tctcctcgaa cccgtcgacc gcgcccagcg catcgaaata gatcgagcgc gccttgcgcg
     3121 gccgcggcgc cggatccgcc gccgaagccc gcatcggcag cccgagcgca gcgcccgcgg
     3181 cgcccgcagc caggagaaat tcgcgacgat ccatgctgct gctcgatgtt agagtgttga
     3241 tacactaata cagttcggcg gcccactcaa gcgggtccga agctgttggg aaacgaggtt
     3301 cggctggcgc tcgagaaaac ggggcgctaa gctgccgtgg aggagtaagt catgcaggtc
     3361 actgccattc tgacgcctgc ggaggagggc ggctacgtcg ctttcaaccc cgagacaggg
     3421 acgacgacac agggggagtc ggtggaggag gcgctggcca acctgcgcga ggcggtcgag
     3481 ctgtatcgtg aagagttttc cagttaagag gggcagccca tggtggatat gagcattctc
     3541 gttggaagcg tccggcgctt cggtctcgtc ggccctccct atgaggtgct cggtccttcc
     3601 ttgccgtcgg aagcgggcga gccgcagatg cgcatcctcc tcatagagag cggcgaagag
     3661 gtggattatc cggtgagcga ggtcgtcaac gatcccgtcg atgcctgatg ttcgcgatcg
     3721 ggtttgacct gctatggcac cagacgaggc agacgcaccc caagggtgtg agccaagcct
     3781 atgccgacat cggtgcggtc ctcaaccgct acggcttctc gcgcgtgcag gggagcgttt
     3841 atgtctccca tggagacagc ctcgtcaacg tcacgctcgc tatgaatgcg ttgaaggcgc
     3901 tgccctggtt tccgaattgc gtgcgcgata tccgggcctt caaggtcgag cattggagcg
     3961 actttaccga cttcatgaag acgtgaggaa gcgaaggtga cagacgccgc ccgtctcacc
     4021 acccccgatc gccggcccga ggacctcgac gccgcgcttc ggccgaagac gctcgacgag
     4081 ttcgttgggc agcgcgccgc ccgcgagaat ttgcgggtgt tcatcgatgc cgcgcgccat
     4141 cgcggcgatg cgctcgacca tgtcctgttc ttcgggccgc cggggctcgg caagacgacg
     4201 ctggcgcaga tcgtcgcgcg cgagcttggc gtcggcttcc gcgccacgtc gggcccggtg
     4261 atcgccaaat cgggcgacct cgccgccttg ctcaccaatc tcgaggatgg cgacgtcctg
     4321 ttcatcgacg agatccaccg cctcaatccg gcggtcgagg aggtgctcta tccggcgatg
     4381 gaggaccgcg cgctcgacct gatgatcggc gagggccctt cggcccggtc ggtgcggatc
     4441 gaccttccca aattcacttt ggttggcgcg accacgcggc aggggctgct gacgacgccg
     4501 ctccgcgacc gttttggcat tccggtccgg ctcaatttct acagcgtcga ggagctcgag
     4561 caggtggtgc ggcgcggggc gcgcctgatg gggctggacc tcaccgagga cggcgccacc
     4621 gaggtggcgc ggcgctcgcg cgggacgccc cgcatcgccg ggcgcctgct ccgccgggtc
     4681 cgcgatttcg ctcatgctgc cggcgagctc cgcgtcgacg cccgcgttgc cgacgccgcg
     4741 ctgacgcgga tggaagtgga cgcgctcggg ctcgacgcga tggaccgccg ctacctgacg
     4801 atgatcgccg acatctacaa aggcggcccg gtcggcgtcg agacgctcgc cgccgggctt
     4861 tcggagccgc gcgacacgat cgaggaggtg atcgagccgt tcctgatcca gctcggcctc
     4921 gtcgcccgca ccgcgcgcgg ccgctgcctc aacggccgcg cctggaccca ccttggcatc
     4981 gttcctccga aaggcgcgca ggacgggctg ttcgactcga attgagggcg ctgcggcgca
     5041 aaaacgtctc gcctcgcccg ttcatccttc cgccgcgaat ccggcgtggt taacactcgc
     5101 gggcgtgagc ggggacggga tcgatacacc ttatcaaggc ggcttcgtgg gcaagaccca
     5161 ccgcttcgcg ctacgcgtct atttcgagga caccgacacg gccggcatcg tctattatgc
     5221 caattatctg cgcttcatgg agcgggcgcg ctccgacatg ctgcgcgcgg tcgggatcga
     5281 ccagcgggcg gcgctcgaag gcggcgacgg cgtctatgcg gtcgccgaag cgtatatcaa
     5341 atatcgcgcc tccgcccggc tcgacgacgc gctggtcgtc gtcagccatg tgcgcgaggt
     5401 gcgggcggcg agctgcgtga ttcagcagca ggtcatgcgc ggaagcgaaa tcctgaccca
     5461 cgccaaggtg acggccgcct tcctgtcgcc cgacgggcgt cccaggcgcc agccgcgcgc
     5521 gtgggtggag atattcgagc ggttgaaagg ggaattggaa gcgtgaccga catgggattg
     5581 accggcgggg caagcgagat gatgtccccg gtcgccttgt tcctgcaggc cgacatcgtc
     5641 gtcaaggcgg tgatgatcgg gctcatcctg gccagcatct ggacctgggc gatcatcctg
     5701 tcgcactggc tgcgcgtcag gcgggtcggc cgcgagaatg acaagttcga gcgggagttc
     5761 tggaaagccg aggatatcga ccgcttcaac gatgcgcgcg gcggcgacga ctggccgagc
     5821 gccaaggtgt tcgccgcggg gatcgccgaa tggcgccggt ccaccgccgg ccgcaatgtc
     5881 gacaaggaag gcacccgctc ccgcctcgcc accgccatgc atgcggcggt gggggcggag
     5941 gtggaccggc tcgccgaccg gctcaacgtt ctcgccacga tcggctcggt ggcgccgttc
     6001 gtggggctgt tcggcacggt ctgggggatc atgcgcagct tcagcgacat tgccggcgcc
     6061 aacaacacca gcctcgccgt ggtcgcgccc ggcatcgccg aggcgttgtt cgcgaccgcg
     6121 ctgggcctgt tcgccgcgat accggcggtg atcgcctaca atcgcatgac ccacggcatc
     6181 aaccggatcg aggccaagct cagccgcttt gccgacggct tccacggcac gctcagccgg
     6241 gaattggagg ccgaggcatg agcggggggt cgaaatcctc cccggaacgg ggagggggac
     6301 cggcgaagcc ggtggaaggg tgcgagcgta gcgagcgtgg aggcgcgggc cccctccacc
     6361 atactgcgca tggtccccct ccccgttccg gggaggaatc ataatggcca tgggtccgat
     6421 ccatagcggc ggacagcggc ggcggcggac gccaatggcg gagatcaacg tcacgccgat
     6481 ggtcgacgtg atgctggtgc tgctgatcat cttcatggtg accgcgcccc tgctggtcgc
     6541 cggcgtgccc gtcgacctgc ccgagaccaa ggccggcgcg ctcgatcagg agcagaagcc
     6601 ggtccagata tcgctggagg cgagcgggtc gatcttcatc gacgacgcca aggtgacgga
     6661 ggccgagctc ggccaaaggc tggcgcagat cgccggcgcc agcgtcgaag agggcggccc
     6721 gcgcatcttc ctgcgcgccg accgcggcct cgattacggc cgggtgatgc gggtgatggg
     6781 cgagatcaac gccgccggcc tgcgccgggt cgccctcgtc agcacccaag gccaggaggg
     6841 ctgatggacc gcgccgaagc caccggatta ggcgctgcga cgctcggcca cgcggccctg
     6901 ctcgcggcgc tgacgctcgg cttcgccaat gtcgtgcggc cgccggtctt gaacgatccg
     6961 atggaggtcg agttcgtcga cgaggtcggg ctggaaagcg ccgcgccggt gccttcgccg
     7021 gccgaggcgg cgccgcggct cgccgaggtc gaggggccgg tcgaaccggt ggcgccgccg
     7081 ccgacaccgc cgccgcccgc ccccgtcccc gagccgctgc cgcagcccaa ggcgatcaag
     7141 cccgcgcccg cgcccgcgcc caaagcagtc gagcgcccga agccgcccaa gcccgcaccc
     7201 gccaaagcca aggcagctcc cgccaaggcg cctccccaga aggcggcccc tgccaaggcc
     7261 gcaccgccca agcaggacag cgccgccaaa gccaaatcgc agcccaaagg gcgcctcagc
     7321 ggcatcctca gcggcatcag cgaccgcgag accaccagcc gctcgacggc gccgcccgct
     7381 caaaaggcgg gcccggcggt gcaggcctcg ctggtcgccg cggtgcgccg ccaggtgaag
     7441 ccgcattgga aagcgccgac cggcgccgac gtggagctgc tccgcaccga gctcagcatc
     7501 cgcctcgcca aggacggcag ggtcaccgac atcgacgtgc ttcgcaccac cggtcagaat
     7561 gccagcaacc gcccgcaggt gaagctccac caggagcagg cggtgaaggc ggtgcggctg
     7621 gcctcgccct tcaatcttcc ggccgaatat tacgacgcct ggaagcatct cagccccttc
     7681 ggattcgaca aaaggctatc gcaatgaccc gaatgaagct cctgctcgga ggcctcgccg
     7741 caacattcct ggccgcattg cagccggccc atgcgcagct ggccgtcgat gtcacggacg
     7801 aaagcggcaa cgacatgatc atcgccgtgc ctgcgatgcc gacgtcgcaa agcgctccga
     7861 ctccggccgg caccaccgac gccctcggcc ggcagatagc cgaggtggtg gtggcggacc
     7921 ttcgcggcag cggcctcttc cagccccgcg gcccggggga ggtccgcgcc attggttatc
     7981 cggaagtcac cgcgccggcg tacgattatt ggccgggcac cggcgccgtc gcgctcgtgc
     8041 agggcttcgt ccgcgccaat ggcgacggca agatcaccgt cggctgctac ctgtacgacg
     8101 tcggcctgcg ctcggagctt acccgtcagg gctatgtgat cgagccgcgc gagtggcggc
     8161 gcgccgccca taaatgcgcc gacgccatct attcgcgcct gtccggggaa agcccgttct
     8221 tcgacagccg gatcgcctat atcgccgaat ccggtccgaa agcgcggcgc gtgaagcggc
     8281 tggcggtgat ggattcggac ggcgccaatc accgcttcct caccaacggc caatcgatcg
     8341 tgctcacccc ccgcttctcg cccgattacc ggcatatcgt ctacatgagc tactccgagc
     8401 ggcggccgag catttatatt tacgataccc tcaccgatcg gcagcgggtc gtcactgaga
     8461 gcgccaacca ggccttcgcg ccccgtttct cccctgatgg ccgctacatc ctc
//
LOCUS       DASVRK010000164         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_31484, whole genome shotgun sequence.
ACCESSION   DASVRK010000164 DASVRK010000000
VERSION     DASVRK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31484"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VF589_06415"
     CDS             <1..1026
                     /locus_tag="VF589_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257248.1"
                     /translation="DLVSGVARGNYFNVVILGSDRSDTFDETASTENYYINGGMGNDR
                     LIGGGGRDFLVGGAGDDRLIGRQSADSFIGGAGDDFILGAVGNDRAFFNVSTDGSDRT
                     NLGSGQDVVEVSANEPGQVRLTFTSSEVGDRNADDSNTGENQDGGLAVRLQLEGADDT
                     LTGSVSRFDDEGITFRSTTEGVTFDVRDLTSGTERGDQFDAVVLGTSRRDGFNFIRDE
                     ENYYVNGGQGDDRIVGGRSDDFLVGGAGNDRLTGKGGDDMMIGGGGEDTFVFNGAPGE
                     DTILDFTSGSDQIDLSAYGITSDDVDTQASGDDTIVRVDANQDGNFDFRIILENSAPP
                     AQTDYVF"
     gene            complement(1120..2097)
                     /gene="tilS"
                     /locus_tag="VF589_06420"
     CDS             complement(1120..2097)
                     /gene="tilS"
                     /locus_tag="VF589_06420"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX8257249.1"
                     /translation="MPSLAPSPDQLARFRRDLATLGESGRLGVAVSGGPDSLALLLLA
                     AAALPGEVEAATVDHGLRPASAVEALHVEDICARIGCPHTILEAAVPDGPAGLQGEAR
                     GARYAALAGWAGRRGLSRLATAHHADDQAETMLMRLRRGSGVAGLSGIRPVRAEGDLL
                     VIRPLLGWTKAELVHLVASAGIEAADDPSNRDPRFDRAAIRRLLAEHPQFEPHRLARS
                     AAACREADEALAWSAAELAEDRITSQGGEWRVDPTGLPRELRRRLLARALAEIRAANG
                     LEPPWTGGEDVEGLLAALEAGGASTLAGVMARGGGPAWQLRPAPPRRSG"
BASE COUNT          396 a          846 c          885 g          326 t
ORIGIN      
        1 gacctggtca gcggcgtcgc ccgcggcaac tatttcaacg tcgtcatcct cggctccgac
       61 cgcagcgaca cgttcgacga aaccgcgagc acggaaaatt attacatcaa cggcggcatg
      121 ggcaacgacc ggcttatcgg cggcggcggc cgtgacttcc tggtcggtgg agccggcgac
      181 gaccggctga tcgggcggca gagcgccgac agcttcatcg gcggcgccgg cgacgatttc
      241 atcctgggag cggtcggcaa cgatcgcgcc ttcttcaacg tctccaccga cggtagcgac
      301 cggaccaatc tcggctccgg gcaggacgtg gtcgaagtgt cggccaacga gccgggccag
      361 gttcggctga ccttcaccag ctcggaagtc ggcgatcgta acgcggatga cagcaacacg
      421 ggcgagaatc aggacggcgg cctcgccgtg cgcctgcagc tcgagggcgc ggacgacacc
      481 ctgacgggct cggtcagccg cttcgacgat gaaggcatca ccttccggag caccaccgaa
      541 ggcgtcacct tcgatgtccg ggatctgacc agcggcaccg aacgcggcga ccagttcgat
      601 gcggtcgtgc tcggcaccag ccgccgcgac ggcttcaact tcatccgcga cgaggaaaac
      661 tattatgtga acggcgggca gggtgacgac cggatcgtcg gcggccgcag cgacgacttc
      721 ctggtcggcg gcgcgggcaa cgaccggctg accggcaagg gcggcgacga catgatgatc
      781 ggcggcggcg gcgaagacac gttcgtcttc aacggcgcgc ctggcgagga caccatcctc
      841 gacttcacgt cgggctccga ccagatcgac ctcagcgcct acggcatcac gtcggacgac
      901 gtcgacacgc aggcctcggg cgacgacacg atcgtgcgcg tcgacgccaa tcaggacggg
      961 aatttcgact tccggatcat cctggagaat tcggccccgc ctgcccagac cgactacgtc
     1021 ttctgacgaa aaggcgtctc ctcccggctc tgccgggagg gtcgtcatga agaaactaca
     1081 gctccgccgg cgctcgcgcc ggcggagttt tgatgtggct cagccggagc ggcgcggcgg
     1141 ggccggccga agctgccagg ccgggccgcc gccgcgggcc atcacgccgg ccaaagtcga
     1201 ggcgccgccg gcttccagag cggcgagcaa gccttcgaca tcctcgccgc cggtccaggg
     1261 cggctccagc ccattggccg cgcgtatctc ggcgagcgcg cgggcaagca gccggcgccg
     1321 gagctcgcgg ggaagcccgg tcgggtcgac ccgccattcg ccgccctgcg aggtgatgcg
     1381 gtcctcggcg agctccgcgg ccgaccaggc gagcgcctcg tcggcttcgc ggcaggcggc
     1441 ggcgctgcgg gcgaggcggt gcggctcgaa ctggggatgc tcggccagca ggcgccggat
     1501 cgcggcgcgg tcgaagcgcg ggtcgcggtt cgagggatcg tcggcggcct cgattccggc
     1561 tgacgccaca aggtggacga gctcggcctt ggtccagccg agcaggggac ggatgacgag
     1621 gaggtcgcct tcggcgcgga ccgggcggat cccggacaag cccgcgactc cggagccgcg
     1681 ccgaagccgc atcagcatcg tctcggcctg gtcgtccgca tggtgcgcgg tggcgaggcg
     1741 ggagaggccg cgccggccgg cccatcccgc gagcgcggca tagcgggcgc cgcgcgcttc
     1801 gccctgcagc ccggccggcc cgtccggcac cgcggcttcg aggatggtat gcgggcagcc
     1861 gatgcgggcg caaatatcct cgacatggag cgcttcgacg gcgcttgccg gacgcaggcc
     1921 gtgatcgacg gtcgcggcct cgacctcgcc aggcagcgcc gcggcggcga gcagcaagag
     1981 ggcgaggctg tccggcccgc ccgagacggc gacgccgagc cggccgcttt cgcccagggt
     2041 cgcgaggtcg cgccggaagc gggcgagctg gtccggggaa ggggcgagcg acggcatccg
     2101 cgaagcttta ggggaatagc gggtcagggg gaaatcgggc ggggccgagc gcttttacga
     2161 cgggcgaggg gcgggcggtg aggctctact cgagtctgga cgcagggcgt gaacccgatt
     2221 gcagccgctc cgccggcaca ccgcccgggc gccgagcgga gactcttgca aaataggatt
     2281 tcgctcgttc tacccttcag atggaccaga gccgggaccc cgcacagcgc ggtacgacct
     2341 ccgccggcgg gacgggcact cgtcccgccc cggtactgcc ccgcatccgg acgcgatcga
     2401 tcgtctcctc catcccaagc cgtccggaac gaaccccgtc tcccgctcat ccc
//
LOCUS       DASVRK010000165        14713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_467752, whole genome shotgun sequence.
ACCESSION   DASVRK010000165 DASVRK010000000
VERSION     DASVRK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 14713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14713
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467752"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VF589_06425"
     CDS             <1..973
                     /locus_tag="VF589_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8257250.1"
                     /translation="LKRVVFGQDAAIGVLSSAIKLSRAGLRDPDKPIGNYLFSGPTGV
                     GKTEVARQLASIMGIPLQRFDMSEYMERHSVSRLIGAPPGYVGYDQGGLLTDAVDQHP
                     HSVLLLDEIEKAHPDLFNILLQVMDNGKLTDHHGKTVDFRNVILIMTTNAGASDMARE
                     GVGFGAMTREGEDEEAIKRMFTPEFRNRLDAIVPFAYLPPEVVARVVDKFILQLEMQL
                     ADRNVHISLDEESKKWLTARGYDRLYGARPMGRLVQEKIKQPLAEELLFGKLMHGGEV
                     KVRIKDNAPSFEIIAAPPKGRKKKKGQPEAEQGEQGQPEEGQPEAQA"
     gene            1122..1763
                     /locus_tag="VF589_06430"
     CDS             1122..1763
                     /locus_tag="VF589_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8257251.1"
                     /translation="MAGSITFYTNPMSRGRIARWMLEEVGEPYETVLLDYATTMKSPD
                     YLAINPMGKVPAIRHGETVVTEAAAICAYLAEMFPDKGLAPPPGNQRRGPYYRWMFFA
                     AGPVEAAVTGKALGLLAPAEKKAMAGYGSFDETIDALERAVSQSEYICGEQFTAADVY
                     VGSQIGWGLMFGSIDKRPAFQDYVGRLQSRPAARRARELDDAAMPKQDGKEQA"
     gene            1760..2638
                     /locus_tag="VF589_06435"
     CDS             1760..2638
                     /locus_tag="VF589_06435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8257252.1"
                     /translation="MTPETRSAAIALYDRFTHGGIDRRAFMADLTKLAGSAAAANALL
                     LGIAADPAAAAIVPPNDPRLQTRTLTWELLPGRKMSGYGAAPVNAAPNLPLVLVIHEN
                     RGLNEHIRDVARRVALAGFSAVAPDFLSPSGGTPANEDQARDMIGKLDLATAVADGRA
                     MIQWLNSPAGGSRKVGAVGFCWGGAMVNRLAVAAGPALSAGVSFYGPAPELAEAAKVE
                     AAMLIHLAALDERVNKTGLPWIEALRAAGKRVTGTVHPGVNHAFHNDTSAERYNKPAA
                     EAAWAATVAFFRTHLA"
     gene            2631..3722
                     /locus_tag="VF589_06440"
     CDS             2631..3722
                     /locus_tag="VF589_06440"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8257253.1"
                     /translation="MPKAAPVLALLAALGSAAPAAAAEETLPDRVDRHLRTKASGPFS
                     GVALVAQFGQIWFHRAYGHASAEASVPNATNTRFAIASLTKPITAAAVLRQVELGKIR
                     LDASICDYLTACPRRWKPVTIRHLLSHTSGLPDLFEKVSPSSATTFRPAIDAAVAAHG
                     AGALSSRPGSRYAYNNFNYFLLGYALEAVTGESWDAAVRGAVLAPAAMAGTRYDVKPP
                     AAPGAASGYRRVDAGRVIAAPHFSPAAYAAGGLVSTAEDLLRFEGALSGGKIVEAGTL
                     QLMTRPGLGRYGLGWQVLKQFGRMQRNHTGQLPGYASHIARYDDGTTIILLSNVDNEP
                     AQDTACEIAALSFGLPLPEPGKPACPPAA"
     gene            complement(3783..4493)
                     /locus_tag="VF589_06445"
     CDS             complement(3783..4493)
                     /locus_tag="VF589_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX8257254.1"
                     /translation="MTAAPRTADVGREGVAFDAGLRSYMLSVYNYMTSGVLLTGIVAL
                     LFSNWSGAPAILFGPGILKYVIMFSPLAFIMVLSFGINRLSTGTAQMLFWAFAAVMGV
                     SLSSIFFVFTQQSIATTFFATAAAFASLSLWGYTTKKDLSGWGTFLIMGVFGLIIAMV
                     INMFVGSTAMQMGISFIGVLLFAGLTAYDTQRIKSLYFQVAGSDMMGKTAIMGALSLY
                     LDFVNMFTFLLQFMGSRE"
     gene            4639..5172
                     /gene="thpR"
                     /locus_tag="VF589_06450"
     CDS             4639..5172
                     /gene="thpR"
                     /locus_tag="VF589_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX8257255.1"
                     /translation="MHRLFVAIRPPTEIRERLLDLMEGVDGVRWQDEEQLHLTIRFIG
                     EVDRHAAEDIAAALANLRHPRFDLALDGIGSFDRRGQAATLWAGVAPQEPLKALHKKV
                     DQAVQRAGAAPDRRAYMPHITLARLGRSAGSIRTLLESHGGLSSPRFEVDHFRLYESS
                     LSPKGAVYTIVARYPLD"
     gene            complement(5182..5532)
                     /locus_tag="VF589_06455"
     CDS             complement(5182..5532)
                     /locus_tag="VF589_06455"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEX8257256.1"
                     /translation="MRKTGTIGLILAAGMSLGACTTHDRYGYGYDRYGDNNRTLGRAA
                     TGAAVGAAAGAGVGALVGGVGVGEGAAVGAVAGGLIGAATANGDRRWYRDDYGRCFYV
                     DRGGYRRYDDRVRC"
     gene            complement(5680..6489)
                     /locus_tag="VF589_06460"
     CDS             complement(5680..6489)
                     /locus_tag="VF589_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX8257257.1"
                     /translation="MTAFGARVADLTGVPAERLERLSGGDLSEVLLLRRPDGRCTVAK
                     SGPAIATEAAMLRSLAGAGVPVPSVEGEHDGILLLEYVENDRVFSAAVWADLGAALRR
                     LHARSGERFGWPVDYALGTVALDNRQTDDWPAFWGEQRLVATASVLDRPWRERVDRLA
                     GRMAQLLPKVPPPSHLHGDLWEGNILVEGGKLAALIDPACYFGHNEVDLAMLTLFGSP
                     PADFWNGYGRPEPGWEERRRVYQLFPALVHLRLFGSAYAGMVDRLLTRLGA"
     gene            complement(6486..7130)
                     /locus_tag="VF589_06465"
     CDS             complement(6486..7130)
                     /locus_tag="VF589_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8257258.1"
                     /translation="MTLHYHPLSSFCWKVLIALYENGTAFTPKLVDFGDETSRAQFAA
                     LWPVAKIPVLEDGARGRIVPETTIMIEYVDRHYPGPARLLPEDPDLAHAARLADRLFD
                     NYVMAPMQKIVVDRLRPKDRRDPFGVEEARGLLARACTMVDRDMESREWAAGDAFGLA
                     DCAAAPALFYADKVMPLAEPYPHAFAYLKRLEQRPSMARVLAEAEPYFAMFPTE"
     gene            complement(7127..7621)
                     /locus_tag="VF589_06470"
     CDS             complement(7127..7621)
                     /locus_tag="VF589_06470"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX8257259.1"
                     /translation="MGKSVLFVCLGNICRSPLAEAAFRAEAERLGLDVEIDSAGTGDW
                     HLGHPPDPRAAAVAQRNGIDIGHLRARQVSPADFRRFDHILALDAQNLADLEAMRPAD
                     GKAELSLLLDHVAEREGQPVADPYYGGDDHFDATWSDVTEAARGLARKLQTADAESGA
                     GAWH"
     gene            7696..9318
                     /locus_tag="VF589_06475"
     CDS             7696..9318
                     /locus_tag="VF589_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8257260.1"
                     /translation="MATSGTGDPAPAAHPPIRAGAYAWYALGLLVLVYALNFIDRHIL
                     SILAEDIKADLKLHDAQIGFLYGTAFAVFYALFGIPLGRLADSWYRGRLMAIGLALWS
                     AMTALSGFANSFTMLAVARIGVGIGEASASPAAYSMISDYFPKERRATALSIYSSGLY
                     IGGGLSLPIGGFVLSRWNSAWPDPAAAPMGLAGWQAAFLAVGIPGLLLALWVLSLKEP
                     QRGASDGLPQPIVRPNAWRDFGRELLAILPPLTLLSTARLPGELKRNLIMLVCVTAAA
                     LALIWLTGDAPQWGAYCLGVYAVYSWVQSLRHRDPPTYRLLWGTPVTVMLAIAFGSIS
                     FVTYAVSFWTPPYVMRTFYPDPTAPGFYLGGLTGAEEVATIIGWGAALAAATGVILGG
                     VLADLWRRRHPAGRLWVNAMSVVLPIPFAAVMFTTSDLTTFYLLFPFAHLLSSAWVGA
                     AVATLQDLVLPRMRATAGATYILGTTMVGLALGPYYAGKIADVAQNLSAGIFALYAVA
                     PFTLLALWVASRKLGEIEATKVERAREVGEPI"
     gene            complement(9335..9871)
                     /locus_tag="VF589_06480"
     CDS             complement(9335..9871)
                     /locus_tag="VF589_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX8257261.1"
                     /translation="MRRAACIGGGLALAVAVSACTAGGGSAGPGAGDGAASATATLLD
                     PAGRAMGVATATAVGDSVRVRIEGENLPAGSHGAHVHTTGLCTPPKFDSAGPHWNPTS
                     RKHGEQNPSGKHLGDLPNIQIPEGGRGSLEYVIEGTSLAAMLDADGAALVIHATADDL
                     KTDPSGNSGDRIACGAFG"
     gene            complement(9932..11248)
                     /locus_tag="VF589_06485"
     CDS             complement(9932..11248)
                     /locus_tag="VF589_06485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX8257262.1"
                     /translation="MRIAMIGTGYVGLVSGACFSDFGHHVTCVDKDVSKINALRNGTM
                     PIFEPGLDQLVARNVRGGRLAFTTELTDAVGEAEAIFIAVGTPSRRGDGHADLSYVFG
                     AAHEIAAALRTPAVVVTKSTVPVGTGDEVERIIRETAPAAQAWVVSNPEFLREGAAIE
                     DFKRPDRIVVGTDDPQAAELMREVYRPLYLNKAPLLFTSRRTAELTKYAANAFLATKI
                     TFINEIADLCEIVGADVQDVSRGIGLDNRIGPKFLHAGPGYGGSCFPKDTLALLKTAE
                     DYEMPLRIVESVVKVNDARKRAMGRKVIQALGGEARGKTVALLGLTFKPNTDDMRDAP
                     SLAIVQALEDAGARVRGYDPEGMEQARPLMPNVEFCDDPYRAAEGADAVVLVTEWDVL
                     RALNLGQLRKAMAQPILVDLRNIYPPEDAEQAGLRWYGVGRPAREG"
     gene            complement(11472..12596)
                     /locus_tag="VF589_06490"
     CDS             complement(11472..12596)
                     /locus_tag="VF589_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8257263.1"
                     /translation="MRKLAIVVALSSTVLATPALARDGAWYVGGEFGGMIVEEMAIDF
                     RGFDDGDDIVDLDSAIEVDHDIGYDGALFGGYDLGGARVEAEVSYKKADVDALETTVA
                     LPNTRVRYHPIAGGDTTVLSFMINAMLDFGDDDGLQGFVGGGVGYARIKSNNYGVFAN
                     RAPLLDDSDAGLAWQVIAGVRAPLTPNIDVQLKYRFFNADDIKMVGISGNDYESRFRS
                     HSLLGGIIFNFGAAPPPPPPPPPPPPPPPPPPPPPPPPPQIQVGPFIVFFDWDRSDIT
                     PQAAAILDNAASAYQTTGQAQVVLAGHADKSGSADYNVGLSQRRADSVRSYLAGRGVP
                     DGSITTEAFGESRPLVETADGVREPQNRRVEITFGPGSGM"
     gene            complement(12797..13294)
                     /locus_tag="VF589_06495"
     CDS             complement(12797..13294)
                     /locus_tag="VF589_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEX8257264.1"
                     /translation="MTQSARFKLPYILPGQAQKELFHNEALAALDAALHAAVEEGAPC
                     ADPPPSPVPGQGWIVAAPGAGEWSGRGQQLAVWTEAGWRFVAPVAGMLVWDKAAGHWL
                     HFDGAAWSGGELRAAAIVVAGQQVVGTRQPHVPSPSGGTIIDGEARAAIEAVIATLIS
                     HGLIE"
     gene            complement(13307..>14713)
                     /locus_tag="VF589_06500"
     CDS             complement(13307..>14713)
                     /locus_tag="VF589_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8257265.1"
                     /translation="EAVEAGATGAKGGGGKTELARRAAGAIPGEVSVAYHDPARDYQT
                     GLQRATRGLGGQDAAGVRAERIALPAVLQAGAAKAIAERRLAQVWAGRASAKLHLGWR
                     RCGVRPGARVRIEGEAGLWRVQRWTLQEMVLTLELVRVRSGRETETSGATPGRPVGHP
                     DLLHGATSVKLLDLPLTASGAGERPLLLVAAAGAEPGWRGAAVTASFDGGASWESAGR
                     TAAPAVMGQALSALGGGGPALIDARGSVEVELLNSNMWLEGRSDAALAGGANLALLGD
                     ELIQFGEAEPSGPRRFRLSRLVRGRRGTEWACALHQAGEDFLLIERAALLALDVPVSA
                     IGGEARVLAKGLGDAAPAEAACPIRGEALRPPSPVHLGAAFEPNGDLRLSWVRRTRSG
                     WAWLDGGETPLGEERELYRLRLSAPGLERSVEVGEPAYVYAAAQRIADGAAGPVTFSV
                     SQVGTYAASRPAILVVEL"
BASE COUNT         2335 a         5349 c         4746 g         2283 t
ORIGIN      
        1 cctcaaacgg gtcgtgttcg gccaggatgc ggcgatcggc gtgctatcct cggcgatcaa
       61 gctgagccgc gccgggcttc gcgatcccga caagccgatc ggcaactacc tcttctccgg
      121 ccccaccggc gtcggcaaga ccgaggtggc gcgccagctc gcctcgatca tgggcatccc
      181 gctccagcgt ttcgacatgt cggaatatat ggagcgccac tcggtcagcc gcctgatcgg
      241 cgcgcctccg ggttatgtcg gctacgacca gggcgggctt ctgaccgacg ccgtcgacca
      301 gcatccgcac agcgtgctgc tgctcgacga gatcgagaag gcgcatccgg acctgttcaa
      361 catcctcctc caggtgatgg acaacggcaa gctcaccgac caccacggca agacggtcga
      421 tttccgcaac gtcatcctga tcatgaccac caatgcgggc gccagcgaca tggcgcgcga
      481 aggggtgggc ttcggcgcca tgacccgcga gggcgaggac gaggaagcga tcaagcggat
      541 gttcacgccg gaattccgca accgcctcga tgcgatcgtt ccgttcgcct atctgccgcc
      601 cgaggtcgtc gcccgggtcg tggacaagtt catcctccag ctcgaaatgc agctggcgga
      661 ccgcaacgtc cacatcagcc tcgacgagga atcgaagaag tggctgaccg cccggggcta
      721 tgaccggctc tacggcgcgc gcccgatggg ccgcctcgtc caggagaaga tcaagcagcc
      781 gctggccgag gagctgctgt tcggcaagct gatgcacggc ggcgaggtca aggtccggat
      841 caaggacaat gcgcccagct tcgagatcat cgcggccccg cccaagggcc gcaagaagaa
      901 gaagggccag ccggaagccg agcaaggcga gcagggccag ccggaagaag gccagcccga
      961 agcccaggcc tgatgatcga agcgcccctc tcctccgggt gatcccggag gagagggaat
     1021 gctcttcccc ctcccctttc cgcctccgcc gccttaaggc tccgctatgt tcggcaggcc
     1081 cggcgcgcta ggctgggcgt gaatcagagg agcggcgccg catggccgga tcgatcacct
     1141 tctacaccaa ccccatgtcc cgcggccgga tcgcgcgctg gatgctcgag gaagtgggcg
     1201 agccgtacga gaccgtgctg ctcgattatg cgacgaccat gaagtcgccc gactatctgg
     1261 cgatcaaccc gatgggcaag gtgccggcga tccgccacgg cgagaccgtg gtcaccgaag
     1321 cggcggcgat ctgcgcatat ctggccgaga tgttccccga caaggggctc gcgccgccgc
     1381 ccggcaacca gcgccgcggg ccttattatc gctggatgtt cttcgccgcc ggcccggtcg
     1441 aggcggcggt gaccggtaag gcgctggggc tgctcgcgcc ggccgagaaa aaggcgatgg
     1501 cgggctatgg cagcttcgac gagaccatcg acgcgctcga gcgggcggtc tcccagtccg
     1561 aatatatatg cggcgagcaa ttcaccgccg ccgacgtcta tgtcgggtcg cagatcggct
     1621 ggggcctgat gttcggctcg atcgacaaac ggccggcctt ccaggattat gtcgggcggc
     1681 tccagtcgcg gccggccgcg cgccgcgccc gcgagctcga cgacgccgcc atgcccaagc
     1741 aggacgggaa ggaacaagct tgacccccga gacacgctcc gccgcgatcg cgctctacga
     1801 tcgcttcacc catggcggca tcgaccgccg cgccttcatg gccgacctca ccaagctggc
     1861 cggcagcgcc gccgccgcca acgcgctctt gctcggcatc gccgccgatc ccgccgccgc
     1921 cgcgatcgtg ccgcccaacg acccgcggct ccagacccgc accctcacat gggagctgct
     1981 ccccggccgc aagatgagcg gctatggcgc cgccccggtc aacgccgcgc cgaacctgcc
     2041 tctggtcctc gtcatccacg agaatcgcgg cctcaacgag catatccgcg acgtcgcccg
     2101 gcgcgtggct ttggccggct tctcggcggt ggcgcccgac ttcctctcgc ccagcggcgg
     2161 gacgcccgcc aatgaggacc aggcgcgcga catgatcggc aagctcgacc tggcaaccgc
     2221 ggtggccgac ggacgggcga tgatccagtg gctgaattcc ccggccggcg ggtcgcgcaa
     2281 ggtcggcgcg gtcggctttt gctggggcgg cgcgatggtc aaccggctcg cggtggcggc
     2341 cggccccgcg ctcagcgccg gcgtctcctt ctacggcccc gcccccgagc tggccgaggc
     2401 cgccaaggtc gaggcggcga tgctgatcca cctggccgcg ctcgacgagc gggtgaacaa
     2461 gaccgggctt ccctggatcg aggcgttgcg ggcggcgggc aagcgcgtga ccggcaccgt
     2521 ccaccccggc gtcaaccacg ccttccacaa cgacacttcg gctgagcgct ataacaagcc
     2581 cgccgccgag gccgcctggg ccgcgacggt cgccttcttc cgaacccatc ttgcctaagg
     2641 cggcgccggt cctggcgctg ctcgcggcgc tcggctcggc cgcgccggcc gccgccgccg
     2701 aggagaccct gccggaccgc gtcgaccggc acctccgcac caaggcctcg ggcccgtttt
     2761 cgggcgtggc tttggtcgct cagttcggcc agatctggtt ccatcgggcc tatggccatg
     2821 cgagcgcgga agcgtcggtg ccgaacgcca ccaatacgcg cttcgctatc gcctcgctca
     2881 ccaagccgat caccgcggcg gccgtgctgc ggcaggtcga gctcggcaag atccggctcg
     2941 atgcctccat ctgcgactat ctcaccgcct gcccgcggcg ctggaaaccg gtgacgatcc
     3001 ggcacctgct gtcgcacacc tccgggctcc ccgacctgtt cgagaaagtc tcgccctcgt
     3061 cggcgaccac gttccgcccg gcgatcgacg cggccgtcgc cgcccatggc gcgggcgcgc
     3121 tcagcagccg ccccggcagc cgctacgcct acaacaactt caattatttc ctgctcggct
     3181 atgcgctcga ggcggtgacg ggggagagtt gggacgccgc cgtgcggggc gcggtgctgg
     3241 cgcccgccgc gatggccggg acgcgctacg atgtcaagcc gccggcggcg cctggcgcgg
     3301 cttcgggtta tcgccgggtc gacgcgggcc gcgtgatcgc cgcgccgcat ttcagcccgg
     3361 ccgcttatgc cgcgggcggc ctggtctcga ccgcggaaga tctgctgcgc ttcgaagggg
     3421 cgctcagcgg tggcaagatc gtggaggcgg gcacgctgca gcttatgacc aggcccggcc
     3481 tcggccggta cgggctcggc tggcaggtgc tgaagcagtt cgggcggatg cagcgcaacc
     3541 ataccggcca gctccccggc tatgccagcc acatcgcccg ctatgatgac ggcaccacca
     3601 tcatcctgct cagcaatgtc gacaacgagc cggcccagga caccgcctgc gagatagcgg
     3661 cgctgagctt cggcctgccc ctgcccgagc ccggcaagcc cgcctgcccc ccggccgcct
     3721 gaaacccaac gaaaaaggcc cggcgctggg ccgggccttt ctcgtctcgc tcgtgggacc
     3781 gcctattcgc ggctgcccat gaactgaagg aggaacgtga acatgttcac gaagtcgagg
     3841 tagagcgaca gcgcgcccat gatggccgtc ttgcccatca tgtcggagcc ggcgacctgg
     3901 aagtagaggc tcttgatccg ctgcgtgtca taggcggtga ggcccgcgaa cagcagcacg
     3961 ccgatgaagc tgatccccat ctgcatcgcg gtcgagccca cgaacatgtt gatcaccatc
     4021 gcgatgatca ggccgaacac gcccatgatc aggaaggtgc cccatccgga caggtccttc
     4081 ttcgtggtgt agccccacag gctgaggctg gcgaaagcgg ccgccgttgc gaagaaggtc
     4141 gtggcgatcg actgctgcgt gaacacgaag aagatcgacg acagcgacac ccccatcacg
     4201 gccgcgaagg cccagaacag catctgcgcc gttccggtcg acagccggtt gatcccgaag
     4261 ctgagcacca tgatgaacgc cagcggcgag aacatgatga catatttgag gatgcccggg
     4321 ccgaacagga tcgccggagc gcccgaccag ttcgagaaca gcaaagcgac gatgccggtg
     4381 agcagcacgc ccgaggtcat ataattgtag accgacagca tataggaccg caggcccgcg
     4441 tcgaaggcca cgccctcccg gccgacatcc gccgtgcgcg gcgctgcggt cattcgcggg
     4501 tcaaacccat ttgccatttg aacactctcc tttgacggca aaacccttgc cgttagtcgc
     4561 caatatcggg tgaatccgcg tccctttcaa ggaaaaccgg acttggggcg gggcagccgg
     4621 gcgcgtagag gtcccgatat gcaccgcctg ttcgtcgcca ttcgtcctcc aaccgagatt
     4681 cgcgagcggc tgctcgacct gatggagggg gtcgacggcg tccgctggca ggacgaggag
     4741 cagctccacc tcacaattcg cttcatcggc gaggtcgacc gccacgccgc cgaggacatc
     4801 gccgcggcgc tcgccaacct tcggcatccg cgcttcgatc tcgccctcga cgggatcggc
     4861 agcttcgacc ggcgcggcca ggccgccacg ctctgggccg gcgtcgcgcc gcaggagccc
     4921 ttgaaggcgc tccacaagaa ggtcgatcag gcggtccagc gcgcaggtgc cgcgccggac
     4981 cgccgggcct atatgccgca catcaccctc gcccggctcg gccggtcggc agggtcgatc
     5041 aggaccctgc tcgagagcca tggcggcctg tccagcccgc gcttcgaggt cgaccacttc
     5101 cgtctttacg aaagcagcct gtcgcccaag ggcgcggtct atacgatcgt cgcgcgttac
     5161 ccgctcgact gacgcctgag cctagcagcg cacccgatcg tcgtaccggc gatagccgcc
     5221 gcgatcgacg tagaagcagc ggccataatc gtcgcggtac cagcgccgat cgccgttcgc
     5281 ggtcgcggcg ccgatcaggc ctccggccac cgctccgacg gcggcgcctt cgccgacccc
     5341 gacaccgccg accaaggccc cgacgccggc accggcggcg gcaccgaccg cagcgccggt
     5401 cgcggccctg ccgagagtgc ggttgttgtc gccgtagcga tcatagccat agccgtagcg
     5461 gtcatgggtc gtgcaggctc ccagcgacat accggccgca aggatcagcc cgatggttcc
     5521 agttttacgc atacatcacc tccgaagaag attcgccaat ctgccgcctc gtgggaaggg
     5581 cacggcgccc tggccgcggc tggcttttgg agaacgggca cagcccccct tcgttccgga
     5641 atgggcgccc cgggacgccg cccggcgcct ggaatacgct caggcgccga ggcgggtcag
     5701 gagacgatcg accatgccgg cataagccga gccgaacagc cgcagatgca cgagcgccgg
     5761 gaacagctgg tagacgcgcc tgcgctcctc ccacccaggc tcgggccggc cgtagccgtt
     5821 ccagaaatcg gccgggggcg agccgaacaa ggtcagcatc gccaaatcga cctcattatg
     5881 tccgaaatag caggccggat cgatgagggc ggcgagcttg ccgccttcga cgaggatatt
     5941 gccttcccac aggtcgccat ggaggtgcga aggcggcggc accttgggca gaagctgcgc
     6001 catcctcccc gcgagccgat ccacccgctc ccgccacggc cggtcgagca cgcttgcggt
     6061 cgccaccagc cgctgctctc cccagaaagc cggccaatcg tcggtctgcc gattgtcgag
     6121 cgcgaccgtg ccgagcgcat aatcgaccgg ccagccgaag cgctcgccgc tgcgcgcgtg
     6181 aagccgcctg agggcggcgc cgagatcggc ccagaccgcc gcgctgaaca cccggtcgtt
     6241 ctcgacatat tcgagcagca ggatgccgtc atgctcgcct tcgacgctgg gcacgggaac
     6301 gccggcgccg gccaggctgc gcagcatcgc cgcctcggtg gcgatggccg gcccgctttt
     6361 ggcgacggtg cagcgcccgt ccggccgccg gagcagcagc acttcggaca agtcgccgcc
     6421 cgacagccgc tcgagccgct cggcgggaac gccggtcagg tcggcgaccc tggccccgaa
     6481 cgcggtcact ctgtcgggaa catcgcgaaa tacggctccg cctcggccag cacccgcgcc
     6541 atcgacggcc gctgctcgag ccgcttcaga taagcgaaag cgtgcggata aggctcggcg
     6601 agcggcatca ccttgtccgc atagaataag gccggagccg ccgcgcaatc ggccagcccg
     6661 aaggcgtcgc cggcagccca ctcgcggctt tccatgtcgc ggtccaccat cgtgcaggcc
     6721 cgcgccagca gcccgcgcgc ctcctcgacc ccgaacggat cgcgcctgtc cttcggccgg
     6781 agccggtcga cgacgatctt ctgcatcggc gccatgacat aattgtcgaa cagccggtcg
     6841 gcgagccttg ccgcgtgcgc gaggtccgga tcctccggca gcagccgcgc cgggcccgga
     6901 taatgccggt ccacatattc gatcatgatg gtcgtctcgg ggacgatccg gccccgcgcc
     6961 ccgtcctcga gcaccggtat cttggcgacc ggccacagcg cggcgaactg cgcccgcgaa
     7021 gtctcgtcgc cgaaatcgac cagcttgggg gtgaaggcgg tgccgttctc gtaaagcgcg
     7081 atcagcacct tccagcaaaa ggaggacagc gggtgataat gcagcgtcaa tgccatgcgc
     7141 ctgcccctga ttccgcgtcg gccgtctgca gcttgcgggc gagccccctg gccgcctcgg
     7201 tcacgtccga ccaggtcgcg tcgaaatggt cgtcgccgcc ataataagga tcggcgaccg
     7261 gctgcccttc ccgctccgcg acatggtcga gcagcagcga cagctcggcc ttgccgtcgg
     7321 ccggccgcat cgcttcgagg tcggcgaggt tctgcgcgtc gagcgctagg atatggtcga
     7381 accggcggaa atcggccggc gagacctggc gcgcccgcaa atggccgatg tcgatcccgt
     7441 tcctttgcgc caccgccgcc gcgcgcgggt cgggcggatg gccgagatgc cagtcgccgg
     7501 tgccggcgga atcgatctcc acgtcgagcc cgagccgctc cgcctcggcg cggaacgccg
     7561 cctcggcaag cggcgaacgg cagatattgc cgagacagac gaacagcacc gacttcccca
     7621 tcgactaacc cttgttgcca aaagctcggc ctctgctagc cctgtgacgg gcgcggcaca
     7681 acgggagggc tgggaatggc gacgagcggc acgggcgatc cggctccagc ggcgcacccg
     7741 ccgatccgcg ccggcgccta tgcctggtac gcgctcggcc tgctggtcct ggtctatgcg
     7801 ctgaatttca tcgaccgcca catcctctcg atcctcgccg aggacatcaa ggccgacctc
     7861 aagcttcacg acgcccagat cggcttcctc tacggcaccg ccttcgcggt cttctacgcc
     7921 ttgttcggca ttccgctcgg gcggctcgcc gacagctggt atcgcggccg gctgatggcg
     7981 atcggcctcg ctttatggtc ggcgatgacc gcgctttccg gcttcgccaa cagcttcacg
     8041 atgctcgcgg tggcgcgcat cggggtcggc atcggcgagg cgagcgcatc gcccgcggcc
     8101 tattcgatga tctcggatta tttccccaag gaacgccgcg ccaccgctct ctccatctat
     8161 tcgagcgggc tctacattgg cggcggcctc agcctgccga tcggcggctt cgtgctcagc
     8221 cgctggaaca gcgcctggcc ggatccggcg gcggcgccga tggggctcgc cggctggcag
     8281 gcggccttcc tcgccgtcgg cattcccggg ctcctgctcg cgctctgggt gctgtcgctg
     8341 aaggagccgc agcggggcgc gtcggacggc ctgccgcagc cgatcgtccg acccaacgcc
     8401 tggcgcgatt tcggacgcga gctgctcgcc atcctgccgc cgctcacgtt gctcagcacc
     8461 gcgcgccttc ccggcgagct caagcgcaac ctgatcatgc tcgtttgcgt caccgccgcg
     8521 gcgctcgcgc tcatctggct gaccggggac gctccgcaat ggggcgccta ctgcctcggc
     8581 gtctatgccg tctattcgtg ggtccagtcg ctccgccacc gcgatccacc cacctaccgg
     8641 ctgctgtggg gaactccggt cacggtgatg ctcgcaatcg ccttcggcag catcagcttc
     8701 gtcacttacg ccgtcagctt ctggactccg ccttatgtga tgcggacctt ctatccggat
     8761 ccgacggcgc cgggtttcta tctgggcggc ctgaccggcg cggaagaggt cgccacgatc
     8821 atcggctggg gcgccgccct ggcggcggcg accggcgtta tcctcggcgg cgtcctcgcc
     8881 gacctctggc gccgccgaca ccccgcgggc cggctctggg tcaacgccat gtcggtggtc
     8941 ctgccgatcc cgttcgcggc ggtgatgttc acgaccagcg acctcaccac cttctacctg
     9001 ctcttcccct tcgcccacct gctcagttcg gcctgggtgg gggcggcggt ggcaaccctg
     9061 caggatctgg tcctcccgcg catgcgggcg acggccggcg cgacctatat tctcggcacg
     9121 accatggtcg gtctcgcgct cgggccttat tatgccggca agatcgccga cgtcgcccag
     9181 aatctcagcg ccggcatctt cgcgctctac gcggtcgccc ccttcacgct gcttgccctt
     9241 tgggtggcgt cgcgcaagct cggcgagatc gaggcgacca aggtggagcg ggcgcgcgag
     9301 gtcggcgagc cgatctaact cgcccgccgc atcctcagcc gaacgctccg caggcgatgc
     9361 ggtcgccgct attgccggaa gggtccgtct tgaggtcgtc ggcggtggcg tggatcacga
     9421 gcgcggcgcc atccgcgtcc agcatggccg ccaggctggt gccctcgatc acatattcga
     9481 ggctgcctct gcccccctcg gggatctgga tattgggcag gtcgccgaga tgcttgccgg
     9541 agggattctg ctcgccatgc ttgcggctgg tcgggttcca gtgcggcccc gcgctatcga
     9601 atttcggcgg cgtgcaaagc ccggtcgtat ggacatgcgc gccgtggctg cccgccggca
     9661 aattctcgcc ctcgatccgc acgcgcacgc tgtcgccgac cgcggtggcg gtcgcgacgc
     9721 ccatcgcccg gccggcggga tcgagcaacg tcgccgtcgc cgaagcggct ccatcccccg
     9781 ctccgggacc ggcggagccg ccgcccgccg tgcaggccga taccgccacc gccagtgcga
     9841 ggccgccccc tatgcaagcc gctcgcctca tcgtctctct ccttttgctg tcctgcacga
     9901 acgagccgaa ggtgaaacgg tttcgagggc ctcagccttc ccgcgccggc cggccgacgc
     9961 cgtaccagcg cagccccgcc tgctccgcat cctcgggcgg gtagatattg cgcaggtcga
    10021 ccaggatcgg ctgcgccatc gctttgcgaa gctggcccag gttgagcgcg cgcagcacgt
    10081 cccattcggt caccagcacc accgcgtccg ctccttcggc ggcgcgatac gggtcgtcgc
    10141 aaaattccac gttcggcatc agcgggcgcg cctgctccat gccctcgggg tcatagccgc
    10201 gcacccgcgc cccggcatcc tcgagcgcct gcacgattgc cagcgaaggc gcgtcgcgca
    10261 tgtcgtcggt attgggcttg aaggtaagac cgagcagcgc gaccgtcttg ccccgggcct
    10321 cgccgccgag cgcctggatc accttgcggc ccatcgcgcg cttgcgggcg tcgttgacct
    10381 tcaccactga ctccacgatg cgcagcggca tctcataatc ctcggcggtc ttgagcagcg
    10441 ccagcgtgtc cttggggaag cacgacccgc catagccggg gccggcatga aggaatttcg
    10501 gcccgatgcg attgtcgagc ccgatcccgc gcgacacgtc ctgcacgtcc gcgcccacaa
    10561 tctcgcacag atcggcgatc tcgttgatga aggtgatctt ggtggcgagg aacgcattgg
    10621 ccgcatattt ggtgagttcg gcggtccggc ggctggtgaa gaggagcggg gccttgttga
    10681 ggtagagcgg gcgatagact tcgcgcatca gctccgccgc ctgcgggtcg tcggttccca
    10741 ccacgatccg gtccggccgc ttgaagtcct cgatggccgc gccttcgcgg aggaattccg
    10801 gattggagac cacccaggcc tgggcggcgg gcgccgtctc gcggatgatc cgctcgacct
    10861 cgtcgccggt gccgaccggc acggtcgatt tggtcaccac aaccgccggc gtccgcagcg
    10921 ctgcggcgat ctcgtgggcg gccccgaaga cgtagctaag gtcggcatgg ccgtcgccgc
    10981 gccgcgacgg cgttccgacc gcgatgaaga tcgcctccgc ctcgccaacc gcatccgtca
    11041 gctcggtagt gaaggccagc cgtccgcccc gcacgttgcg cgcgaccagc tggtcgagcc
    11101 caggctcgaa gatcggcatg gtgccgttcc gcagcgcatt gatcttcgac acgtccttgt
    11161 ccacgcaggt gacgtggtgg ccgaaatcgg aaaaacaggc cccggatacc agcccgacat
    11221 agccggtccc gatcattgcg atacgcatgg aacgtccctg aacagatgaa gcccccgtcg
    11281 cttagcaaag ctcaggcaac gcgaacaggt ttcattccgg acggcatttt gccggctcgt
    11341 ttcaggacag cttagcaggc acttgaacgg cgccgttcaa gccacgcgca aaaaagaagg
    11401 gagccggccc gaaagccgac tccctctttt cactttcagg cgtcccgccg ccggagcgac
    11461 gggaaccttt attacatgcc cgaacccgga ccgaaggtga tttccacgcg ccggttctgc
    11521 ggctcgcgga caccgtcggc ggtttcgacc agcggacggc tctcgccgaa cgcttcggtg
    11581 gtgatcgaac cgtccggcac accgcggccg gcaaggtacg aacggaccga gtccgcacga
    11641 cgctgcgaga ggcccacgtt gtagtccgcc gaacccgact tgtccgcgtg accggccaga
    11701 acgacctggg cctggcccgt cgtctggtag gccgaagcgg cgttgtcgag gatcgcggct
    11761 gcctgcggcg tgatgtccga ccgatcccag tcgaagaaca cgatgaacgg accgacctgg
    11821 atctgcggcg gcggcggagg cggcggcggc ggaggcggag gcggcggagg cggcggaggc
    11881 ggcggaggcg gcggaggcgg agcagccccg aagttgaaga tgatgccgcc gagaaggctg
    11941 tgcgaccgga accgcgattc ataatcgttg ccgctgatgc cgaccatctt gatgtcgtcg
    12001 gcattgaaga agcgatactt cagctgcacg tcgatattgg gggtcagcgg cgcccgcaca
    12061 ccggcgataa cctgccaggc aagcccggca tcgctgtcgt cgaggagcgg agcacgattg
    12121 gcgaacacgc cgtaattgtt cgacttgatg cgcgcatagc cgacaccgcc gccgacgaag
    12181 ccctgcaggc cgtcatcgtc gccgaaatcg agcatggcgt tgatcatgaa gctcaggacg
    12241 gtggtgtcgc cgcccgcgat cgggtggtag cggacgcgcg tattcggcag ggcgaccgtg
    12301 gtctccaggg cgtcgacgtc ggccttcttg taggagactt cggcttcgac ccgggcgccg
    12361 cccagatcat aaccaccgaa caaggcccca tcgtatccga tgtcgtgatc gacttcgatg
    12421 gcgctgtcga gatcgacgat atcgtcgccg tcatcgaacc ctctgaagtc gatggccatc
    12481 tcctcgacga tcataccgcc gaattcgccg ccgacgtacc aagcgccatc tcgcgcaagt
    12541 gccggagtcg cgagcacggt ggatgacagc gccacaacta tggcgagctt ccgcataaat
    12601 ctttcccctt tcttttgcgt cccaaccgga acgaccttga actcaatacg gtaagcggag
    12661 tttccgcgca agcacgctta tccccacata tgttgccgaa atgcctcagt gtacattgaa
    12721 ctcaccgggg cccctcgcca gctgtcccct taaaccagac cgctgcggca atgtttcacc
    12781 cgattggggg attcggtcat tcgatcagtc cgtgtgatat taatgtcgca atgacggctt
    12841 cgatggcggc ccgagcctcg ccgtcaataa ttgttccgcc agaaggactt ggcacgtgcg
    12901 gctggcgcgt tccgacgacc tgctggccag cgacgacgat cgcggcggcg cgcaattctc
    12961 cgccgctcca cgccgcaccg tcgaagtgga gccaatggcc ggcggctttg tcccagacga
    13021 gcatgcccgc gaccggcgcg acgaaccgcc acccggcctc ggtccagacc gcgagctgct
    13081 ggcccctgcc gctccattcg cccgcccccg gtgcggcgac gatccacccc tgccccggga
    13141 ccggcgacgg cggggggtcg gcgcacggcg cgccttcctc caccgccgcg tgcaacgccg
    13201 cgtcgagcgc cgcgagcgct tcgttgtgga acagctcctt ctgcgcctgc cccggcagaa
    13261 tatatggtag tttgaacctc gccgattggg tcatcttcca gtcctttcaa agctcgacca
    13321 ccaagatcgc cggtcgcgat gccgcgtagg tgccgacctg gctgacgctg aacgtcaccg
    13381 gcccggcggc gccgtcggcg atccgctgcg cggccgcgta gacataggcc ggctccccaa
    13441 cctcgaccga gcgctcgagg cccggtgccg acagccgcag ccggtaaagc tcgcgctcct
    13501 ccccgagcgg cgtctcgccg ccgtccagcc aggcccagcc gctccgggtc cgccgaaccc
    13561 agctcagccg gagatcgccg ttcggctcga acgcagcgcc gagatgcacc ggcgacggcg
    13621 gccgcagcgc ctcgccgcgg atcgggcagg ccgcttcggc cggcgcggcg tcgccgagcc
    13681 ccttcgcgag cacccgggct tcgccgccga tcgccgagac cggaacatcc agcgccagca
    13741 gcgcggcccg ctcgatcagc aggaagtcct ccccggcctg gtggagcgcg caagcccatt
    13801 cggtgccccg gcggccgcgc accaaccgcg acagccggaa ccgccgcggc ccactgggct
    13861 cggcctcgcc gaactggatg agctcgtcgc cgagcagcgc caaattcgcc ccgcccgcaa
    13921 gcgcagcgtc gctgcggccc tccagccaca tgttcgaatt cagcaattcg acctcgaccg
    13981 atccgcgcgc gtcgatcagc gccggcccgc cgccgccgag cgccgacagc gcctgcccca
    14041 tcaccgccgg cgccgcggtc cggccggcgc tctcccagct ggcgccgccg tcgaagctcg
    14101 ccgtcacggc cgcgccccgc cagcccggct ccgctccggc cgccgccacc agcaaaagcg
    14161 gccgttcgcc cgctccgctc gccgtcagcg gcaggtcgag cagcttcacg ctggtcgcgc
    14221 cgtggagcaa gtcgggatgc cccaccggcc gccccggcgt tgcgccgctc gtctccgtct
    14281 cccgcccgct tctcacccga accagctcga gcgtcagcac catttcctgc agcgtccagc
    14341 gctgcacccg ccacaggccc gcctcgccct cgatgcggac gcgcgcgccc ggccgcacgc
    14401 cgcagcgccg ccagccgaga tggagcttgg cgctcgcccg cccggcccag acctgcgcga
    14461 gccgccgctc cgcgatcgcc ttggccgcgc ccgcctggag caccgccggc agcgcgatcc
    14521 gctccgcccg gacgcccgcc gcgtcctggc cgcccagccc gcgcgtcgcc cgctgcagcc
    14581 cggtctgata gtcgcgtgcc ggatcgtgat aggcgacgct gacctcgccc gggatcgcgc
    14641 ccgccgcccg ccgcgccagc tcggtcttgc cgccgccgcc tttggcgccg gtcgcgcccg
    14701 cttcgacggc ttc
//
LOCUS       DASVRK010000166         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_31611, whole genome shotgun sequence.
ACCESSION   DASVRK010000166 DASVRK010000000
VERSION     DASVRK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31611"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VF589_06505"
     CDS             complement(<1..191)
                     /locus_tag="VF589_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX8257266.1"
                     /translation="MESDSETQMLLWRWQGGESNARDRLIAKLHPELVQIAAARLRGE
                     RDSSLSTGDLINDAIVRLI"
     gene            354..560
                     /locus_tag="VF589_06510"
     CDS             354..560
                     /locus_tag="VF589_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257267.1"
                     /translation="MTADIAVPGACRNCGPVELLLPADHTENSWLDCSVCKQPARTWG
                     DYKKEALDAAADTIRRRRVGSSFR"
     gene            574..939
                     /locus_tag="VF589_06515"
     CDS             574..939
                     /locus_tag="VF589_06515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8257268.1"
                     /translation="MSFSFSYDHFHRFGAARRSERVRTRDLVRLMRDGKPDLQVQLID
                     VSAEGFLLECDELLIAGSTIWIEMPSVRLVSAKVIWQKNGRVGGEFSHRLKWRTLQRF
                     RLVDDTRSSRAVRPGRIFK"
     gene            complement(932..2134)
                     /locus_tag="VF589_06520"
     CDS             complement(932..2134)
                     /locus_tag="VF589_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257269.1"
                     /translation="MGAYQSISEAPAAAEGARAAPEGLRIGLIALAAACVYAAVWPAV
                     TGDMNDYLVPWYDTILARGRVGAFAEPFGNYTPPYLYLLSAVSLFDGLLAKVTLIKLL
                     SVAGVAALAWAVARLVGAAGVERPWRAALWVFLLPSVVLNAPAMGQCDAIWAAACIAA
                     VAETVERRPAAMLVWCGVAIAFKAQAVFIAPFIISRLLAERTPWHLWAIPPLVYGAAM
                     LPAWLMGWPAADLATIYLRQTEWGPDFLGNAPNPWAWAQHFFAEAGAGYRWVGHAAAA
                     AAALAYVVVFGRRRLGARGLVAAALLAAIMLPWLLPKMHERFFFLADLLAFALAAIRR
                     DRIGVAVAVLVQAGSVLALGGLLAASSWPAMVGSVAMLAAAGLTLRCMRGEDAGPEAG
                     QAAERLAI"
     gene            2247..>2487
                     /locus_tag="VF589_06525"
     CDS             2247..>2487
                     /locus_tag="VF589_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257270.1"
                     /translation="MHTRREFAILASAGALAGCSATTGGGGAASLLIPSPPTPPRRTR
                     LALTPVAVAPERVIRTVTGLRPFRPAGFVVRAEPLG"
BASE COUNT          405 a          971 c          792 g          319 t
ORIGIN      
        1 tggatgagcc gcacgatcgc gtcgttgatg aggtcgccgg tcgagagcga ggaatcgcgc
       61 tccccgcgca gccgcgccgc cgcgatctgg accagctccg gatggagctt ggcgatcagc
      121 cggtcgcgcg cgttgctttc gccgccctgc cagcgccaaa gcagcatctg cgtttccgag
      181 tcgctctcca tcgagcctct ccctcgcgcc agcctaggtt cgccgccgcc tcgcggcaac
      241 ccgccgcgcc ccaatccggg aacgccgccc catgcggcaa accgccgcgg ggcgctcaca
      301 tactcttaac cctgtccggc taagctcgcc cctccccaga gtggaaggtg aacatgacag
      361 ccgacatagc cgtacccggc gcctgcagga attgcggccc tgtcgagctg ctcctgcccg
      421 ccgaccatac cgagaatagc tggctcgact gcagcgtctg caagcagccg gcccgcacct
      481 ggggcgatta caagaaggaa gccttggacg cggccgccga caccatccgg cgccgccggg
      541 tcggcagctc tttccgctga accggccccg ccgatgagct tttccttcag ctacgatcat
      601 ttccaccgct tcggcgccgc acggcgctcg gagcgggtca ggacccgcga tctcgtccgc
      661 ctgatgcgcg acggcaagcc cgacctccag gtccagctga tcgacgtctc cgccgaaggc
      721 ttcctgctcg aatgcgatga gctgctgatt gcgggaagca cgatctggat cgagatgccc
      781 tcggtgcggc tggtcagcgc caaggtcatt tggcagaaga acggccgcgt cggcggcgaa
      841 ttctcgcatc gcttgaaatg gcggacgctg cagcgtttcc gcctcgtcga cgacacccgc
      901 tcgtcgcgcg cggtccgccc cggccgcatc ttcaaatagc taagcgctcc gccgcctgac
      961 ccgcctcagg ccccgcatcc tcgccccgca tgcagcgcag cgtcagcccg gccgcggcca
     1021 gcatcgccac gctcccaacc atggccggcc aggatgaggc ggcgagcagc ccgcccagcg
     1081 ccagcaccga ccccgcctgc accagcaccg ccaccgccac gccgatccgg tcgcgccgga
     1141 tcgccgccag cgcgaaggcg agcaggtcgg cgaggaagaa gaagcgctcg tgcatcttgg
     1201 ggagcagcca cggcagcatg atcgccgcca gcaaagccgc cgcgaccagc ccccgcgccc
     1261 cgagccggcg ccggccgaaa accacgacat aggccaaggc cgcggccgcc gccgccgcgt
     1321 ggccgaccca gcgatagccg gcgcccgctt cggcgaagaa atgctgcgcc cacgcccagg
     1381 ggttcggcgc attgccgagg aaatccgggc cccattcggt ctggcgcaga tagatggtgg
     1441 cgaggtccgc cgccggccat cccatcagcc aggccggcag catcgccgcg ccatagacga
     1501 gcggcggaat ggcccagaga tgccaggggg tacgctcggc cagcagccgg ctgatgatga
     1561 acggcgcgat gaaaaccgcc tgcgccttga acgcgatcgc gaccccgcac cagaccagca
     1621 tcgccgccgg cctgcgctcc accgtctcgg cgacggcggc gatgcaggcc gccgcccaga
     1681 tcgcatcgca ctggcccatc gccggcgcgt tcagcaccac cgacggcagc aggaacaccc
     1741 acagagccgc ccgccaggga cgctccaccc cggccgctcc gaccagccgc gcgaccgccc
     1801 aggcgagcgc cgccaccccc gccaccgaca gcagcttgat caaagtcacc ttggccagca
     1861 gcccgtcgaa cagcgacacc gccgacagca aatagagata aggcggcgta taattgccga
     1921 acggctcggc gaacgcgccc acccgccccc gcgccaggat cgtgtcgtac caggggacca
     1981 gataatcgtt catgtcgccg gtcaccgccg gccacaccgc agcatagacg caggcggcgg
     2041 cgagcgcgat caacccgatc cgcagcccct cgggcgcggc gcgcgcgccc tcggccgccg
     2101 ccggcgcttc gctgatgctc tgatacgcgc ccatccgctc cgctccttgc gccggactgt
     2161 gccggttcgt ggttaagaag gcgttgccgg aggcggcgct tcggctctaa gcgcgctagc
     2221 ggcgggcgaa ggagaggcgt tcaacagtgc atacccggcg ggaattcgcg atattggcct
     2281 cggccggggc gctggcggga tgctcggcca ccaccggcgg cggcggcgcg gcctcgctgc
     2341 tcatcccctc gccgccgacg ccgccgcgcc ggacccggct ggcgctgacc ccggtcgcgg
     2401 tggcgcccga acgcgtgatc cgcaccgtca ccggcctccg cccgttccgc cccgccggct
     2461 tcgtggttcg cgccgagccg ctcggcg
//
LOCUS       DASVRK010000167         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_31615, whole genome shotgun sequence.
ACCESSION   DASVRK010000167 DASVRK010000000
VERSION     DASVRK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31615"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VF589_06530"
     CDS             complement(<1..132)
                     /locus_tag="VF589_06530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8257271.1"
                     /translation="MERSVISRHYVLAAGGTGGHMIPAHALAEELTARGHRCALVTDD
                     "
     gene            complement(132..1331)
                     /locus_tag="VF589_06535"
     CDS             complement(132..1331)
                     /locus_tag="VF589_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEX8257272.1"
                     /translation="MNVMQGEARPAKKNQAAKFGRADNSAVGRWFWEIDRVLLLLVAV
                     LISIGLIAVAAASPAAGQRYSGGDISFTPLHYFYRQLMWIVLAVPVMIAVSMLPKAKA
                     RRLCLAGTALFTALLVLVPVIGNEVNGARRWIGFGFAQFQPSEFLKPMFIVSIAWILS
                     LRSEDKNLPVVPLTGILTALIALLLMQQPNFGETIIFAAAWVMLLTLAGVPMRFLVIL
                     GLGAVASVVLAYFFYPVATIRIDSFLFGAAGDADTYQTDSALRTLTAGGLFGAGPGAG
                     IKKFHLPEPHTDYIFSVIGEEFGLIACLAIAILYLVIVARVLIKLLHEEDRFLVLATA
                     GLVTQFGLQALINMAVNVQLAPSKGMTLPFISYGGSSMIALAIGMGLLLAFTRRNPYL
                     HRSPSVV"
     gene            complement(1342..>2129)
                     /gene="murD"
                     /locus_tag="VF589_06540"
     CDS             complement(1342..>2129)
                     /gene="murD"
                     /locus_tag="VF589_06540"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX8257273.1"
                     /translation="SSYQIDLTHSLDCDVSVLLNITPDHLDRYDGVDDYAASKARLFA
                     MQSPEHVAVIAAEDDFTRAIAGQIGGRRVLVGSGDVRDQSRWPSLKGPHNAQNVAAAV
                     AVARELGVSDEAIAAALVTFPGLPHRMERVAEKNGVLFVNDSKATNPTSTAPALAAYP
                     AVHWILGGLPKSDDLDACEPFLDHVKAAYTIGEAGPMFAQLLADRLPVRECEMLVEAV
                     GAAAGAARPGEVVLLSPACASFDQFRDYEARGDAFRAAVEALA"
BASE COUNT          393 a          719 c          715 g          302 t
ORIGIN      
        1 gtcgtcggtg acgagcgcgc agcgatggcc gcgcgcggtc agttcctcgg ccagcgcatg
       61 ggccggaatc atatggccgc cggtccctcc ggcggcgagc acatagtggc gggaaatcac
      121 cgaccgctcc attacacgac agagggggag cggtgcagat aggggttgcg gcgggtgaag
      181 gcgagcagca atcccatgcc gatggcgagc gcgatcatcg acgatccgcc ataggagatg
      241 aacggcagcg tcatgccctt ggacggcgcg agctggacgt tgaccgccat gttgatcagc
      301 gcctggagcc cgaactgggt aaccagcccg gccgtcgcca gcaccaggaa gcggtcctcc
      361 tcgtgcagca gcttgatcag cacccgcgcg acaatgacga ggtaaaggat ggcgatggcg
      421 aggcaggcga tgaggccgaa ttcctcgccg atcaccgaga agatatagtc ggtgtgcggc
      481 tcgggcaggt ggaacttctt gatgcccgcc cccggcccgg cgccgaacag gccgccggcg
      541 gtgagcgtgc gcagcgcgct gtcggtctgg taggtgtcgg cgtcgccggc cgcgccgaac
      601 aggaacgagt ctatccggat cgtcgccacc ggatagaaga aataagcgag cacgacgctc
      661 gccaccgcgc ccagcccgag aatgacgagg aagcgcatcg gcacgccggc gagggtcagc
      721 agcatcaccc aggcggcggc gaagatgatc gtctcgccga aattgggctg ctgcatcagg
      781 agcaaagcga tcaaagcggt gaggatgccg gtcagcggca ccaccggcaa attcttgtcc
      841 tcgctccgga gcgacaggat ccaggcgatc gacacgatga acatcggttt caggaattcg
      901 gacggttgga attgcgcgaa gccgaacccg atccagcggc gggcgccgtt cacctcgttg
      961 ccgatcaccg gcaccagcac cagcagggcg gtgaacaagg cggtgccggc gaggcagagc
     1021 cgccgcgcct tcgccttggg cagcatcgag acggcgatca tcaccggcac ggcgaggacg
     1081 atccacatca actggcggta gaaataatgg agcggcgtga agctgatgtc gccgcccgaa
     1141 taacgctgcc cggccgcggg cgaggccgcc gccaccgcga tcaggccgat cgagatcagc
     1201 accgccacga gcagcaacag cacccggtcg atctcccaga accagcgccc gaccgcgcta
     1261 ttgtcggcgc ggccgaactt ggccgcctgg ttcttcttcg ccggccgcgc ttccccctgc
     1321 atcacgttca ttgccccgtc ctcacgccaa cgcttccacc gcggcgcgga atgcatcccc
     1381 ccgcgcctca taatccctga actggtcgaa cgaggcgcag gccggcgaca gcagcaccac
     1441 ctcgcccggc cgcgccgcgc ccgcggccgc tccgaccgcc tcgaccagca tctcgcattc
     1501 cctcaccggc agccggtcag ccagcagctg cgcgaacatc ggccctgctt cgccgatcgt
     1561 gtaggcggcc ttgacgtggt cgaggaacgg ctcgcacgcg tcgaggtcgt ccgatttcgg
     1621 aagcccgccg aggatccagt gcaccgccgg gtaagcagcc agcgccggcg cggtcgaggt
     1681 cggattggtc gccttggaat cgttgacgaa cagcacgcca ttcttctcgg ccacccgctc
     1741 catccggtgc ggcagcccgg gaaaggtgac gagcgcggcg gcgatcgcct cgtcggagac
     1801 cccgagctcg cgcgcgaccg cgaccgcggc ggcgacgttc tgggcattgt gcggcccctt
     1861 gagcgagggc cagcgcgact ggtccctgac gtccccggac ccgaccagta cccgccgtcc
     1921 gccgatctgg ccggcgatgg cgcgggtaaa atcgtcctcg gcggcgatca ccgcgacatg
     1981 ctccggcgac tgcatcgcga acagccgcgc cttcgacgcc gcataatcgt cgactccgtc
     2041 gtagcggtcg agatggtcgg gcgtgatgtt gaggagcacc gagacgtcgc agtcgaggct
     2101 gtgcgtcagg tcgatctggt aggaggaaa
//
LOCUS       DASVRK010000168         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_93612, whole genome shotgun sequence.
ACCESSION   DASVRK010000168 DASVRK010000000
VERSION     DASVRK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93612"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1720
                     /gene="gspD"
                     /locus_tag="VF589_06545"
     CDS             <1..>1720
                     /gene="gspD"
                     /locus_tag="VF589_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX8257274.1"
                     /translation="SGGAFRIQPVATAATSGGRVGRRSVSPNGFVTEIFRLRNIDAAS
                     AAETVRPLVSREGSVTANRNTLVVADFADNVTRIRQILSGIDRDTASTQVLALQNAGA
                     REIAEALSALVGTGGEGGGAVSVVPIDSSNSIALRGDAATVARLMEIARDLDRRAASG
                     TEVRVIFLENADAEQMLPVLQQLLGQSVTPAATTGSSSRSRLGGWASRNRGGNADASG
                     GGAGGGDNAASIAQTAAPATGGGGGAPGTGTGVFGNKAAIVTRFEGANAIVIAAPADV
                     QRQLGEVIRQLDTRRAQVLVEAIIVEIGDTAAKQLGVQLLLAGTKGSNIPFAVTNYSN
                     ASPNILTLAGAIAAERLRNTTTTVNGQVVTTTQNSGLADTLQEAAAAELLGAAGGIGG
                     VAFRAGNAIFGAIINAVQRDNQSNILSTPSLMTLDNQEARILVGQEVPVTTGEALSDN
                     FDNAFRTVQRQNVGITLEVKPQINAGGAIKLQLRQEVSSIAGPVSNDVSDLILNKREI
                     ETTITVDDGEIVGIGGLLDDNERRTLEKIPFLGDLPLVGNLFKSKGRARSKTNLMVFI
                     RPTIMRS"
BASE COUNT          293 a          607 c          582 g          238 t
ORIGIN      
        1 ctcgggcggc gcgttccgga ttcagccggt ggcgaccgcc gccacttccg gggggcgtgt
       61 cggacgccgg tcggtgagcc cgaacggctt cgtcaccgaa atcttccgcc tgaggaacat
      121 cgacgccgcc tcggccgcgg agacggtgcg gccgctggtc agccgcgaag gctcggtcac
      181 cgccaatcgc aacactttgg tggtggcgga tttcgccgac aacgtcaccc gtatccggca
      241 gatcctgtcc ggcatcgacc gcgacaccgc gtcgacccag gtgctggcgc tgcaaaatgc
      301 cggcgcgcgc gagattgccg aggcgctgtc ggcgctggtc gggaccggcg gcgagggcgg
      361 cggcgcggtt tcggtggtgc cgatcgacag ctccaactcg atcgcgctac gcggcgacgc
      421 ggccaccgtc gcgcggctga tggagatcgc gcgcgacctc gaccgccgcg cggccagcgg
      481 caccgaagtg cgcgtcatct tcctcgaaaa tgccgatgcc gagcagatgc tgccggtgct
      541 ccagcaattg ctcgggcaga gcgtcacgcc cgccgccact acgggatcga gctcgcgctc
      601 gcgcctgggc ggatgggcca gccgtaaccg cggcggcaac gccgacgcga gcggcggcgg
      661 cgccggcggc ggcgacaatg ccgcgtcgat cgcgcagact gccgcgccgg ctaccggcgg
      721 cggcggcggc gcgcccggca ccggcaccgg cgtgttcggc aacaaggccg ctatcgtcac
      781 ccgcttcgaa ggcgccaatg cgatcgtcat cgccgccccg gccgacgtcc agcgccagct
      841 cggcgaagtg atccggcagc tcgatacgcg ccgcgcccag gtgctggtcg aggcgatcat
      901 cgtcgagatt ggcgacaccg cggccaagca gctcggcgtg cagctgctgc tggccgggac
      961 caaggggagc aacattccct tcgccgtcac caattattcc aacgcgagcc ccaacatcct
     1021 gaccctagcc ggcgcgatcg ccgccgagcg gcttcgcaac accaccacca cggtcaatgg
     1081 ccaggtggtg acgacgaccc agaattccgg cctcgccgac acgctgcagg aagcggccgc
     1141 cgcggagctg ctcggcgcgg ccggcgggat cggcggcgtc gccttccggg ccggcaacgc
     1201 gatcttcggc gcgatcatca acgcggtcca gcgcgacaac cagtccaaca tcctgtcgac
     1261 gccgtcgctg atgacgctcg acaaccagga ggcgcggatc ctggtcggcc aggaagtgcc
     1321 ggtgacgacg ggcgaggcgc tgtccgacaa tttcgacaat gccttccgca ccgtccagcg
     1381 ccagaatgtc ggcatcacgc ttgaagtgaa gccgcagatc aatgccggcg gggcgatcaa
     1441 gctgcagctg cgccaggaag tgagctcgat cgccgggccg gtctcgaacg acgtgtcgga
     1501 cctgatcctc aacaagcgcg agatcgagac cacgatcacg gtcgacgacg gcgagatcgt
     1561 cggcatcggc gggctgctcg acgacaatga gcggcgcacg ctcgagaaga tcccgttcct
     1621 cggcgacctg ccgctggtcg gcaatctgtt caaatccaag ggaagggccc gctccaagac
     1681 caatctcatg gtattcatcc ggccgaccat catgcgctcg
//
LOCUS       DASVRK010000169        11029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_281118, whole genome shotgun sequence.
ACCESSION   DASVRK010000169 DASVRK010000000
VERSION     DASVRK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281118"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1471)
                     /locus_tag="VF589_06550"
     CDS             complement(83..1471)
                     /locus_tag="VF589_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8257275.1"
                     /translation="MALDILVVDDEQDIRELVSGVLEDEGYEARTAANSSEALAALAD
                     RRPSLVLLDVWLQGSKLDGLQLLEEVKRRDPSLPVLMISGHGNLDTAVAAIRQGAVDF
                     IEKPFEAGHLLHLVARATETDRLRRENESLRQQIGQETELTGSSVAINAVRATLKRVA
                     GTGSRVLITGPAGVGKEVAARLLHNWSPRAKAPFIVVSAARMTPERVEEELFGVEEGG
                     ELARPGLLEQAHGGTLFLDEIADMPIPAQAKILRVLTDQSFTRVGGQRTVRVDMRVVS
                     STARILADEIAGGRFREDLFYRLNVVPVHLPALAERREDIPELVTHFAARYAAEQRLP
                     TPTLSDDAVAALQAYDWPGNVRQLRNVIERTIILAPSSRVACIEIDMLPPELLNEQAQ
                     LSPGEAVKAIMGTPLREARETFEREYLRVQIRRFSGNISRTATFIGMERSALHRKLKA
                     LGIGDTKGEEGE"
     gene            complement(1476..3710)
                     /locus_tag="VF589_06555"
     CDS             complement(1476..3710)
                     /locus_tag="VF589_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX8257276.1"
                     /translation="MPIVDSRSRLRRRLSIVLRRSPIMPMVEIATVLALIATLTFSYF
                     IVAEQGSPQSLLTPPMVAMLLVANLVPAMALMVLIARRVAMGRAARSPMGGRGRLHVR
                     LVALFSAIASVPTLMVVIFASLLFQQGVQFWFSDRARTILENADNVAQVYAEENRSRI
                     YRDVQAMTVDIVADINQFGLDTPAFAEKLFLQTVYRDLTEAAVISVSRTGDLQLRAGV
                     NLDDRPLEQRFPAPVLSELRGGETRVVTDAGDRVEAVVRLDPEEEVYLYTSRTVNPAA
                     IQQIEEARSAASDYQKTLLNSRTLQFRFNAVLLLVSLLIVALSIWIALTLADRLVRPV
                     GELVDAARRVTAGDLSARVPTSRVNDEVSTLGNAFNRMTRRLEEQTGALVSANNQLDS
                     RRAFIEAVLSGVTAGILSVDTNREVRLINSSAEALLKTGNKSAVGQKLAVLAPELDRQ
                     LDSEEREGIIQFSSGGEPRTLAVKRVKVEGGHVLTFDDITEQLLDQRRAAWSDVAQRI
                     AHEIKNPLTPIQLAAERLQRRYGREISSDGATFERLTGTIVRQVGDLRRMVDEFSSFA
                     RMPKPVFRDEDIADIARQALFLHEVAHPEIRFVLDGPEPSPALVCDRRQLGQALTNIV
                     KNGVEAIQQKNEERGHADSDRIEIRIVEAPGRVTLEISDTGVGLPADRGRLTEPYMTT
                     RVKGTGLGLAIVKKIVEEHSGVIEFDDAPGGGTLVRLIFDAETLARLGGSGEYHSEDQ
                     AMNG"
     gene            complement(3840..4838)
                     /gene="dusB"
                     /locus_tag="VF589_06560"
     CDS             complement(3840..4838)
                     /gene="dusB"
                     /locus_tag="VF589_06560"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX8257277.1"
                     /translation="MSRLAPIAIGPVRIEAPVILAPMTGVTDRPFRRIVKRYGAGLTV
                     TEMIASQAMIRETRQSLQKAAWDPVEEPVSMQLAGCSPAEMAEAAKLNEDRGAAIIDI
                     NMGCPVKKVVNGDAGSALMRQIPLATAIIKATVGAVKVPVTLKMRMGWDHGSLNAPEL
                     ARIAEDLGVRMITVHGRTRCQLYKGEADWRFVRRVKEAVSVPVIVNGDINSIEDAETA
                     LDQSGADGLMIGRGAYGKPWLLGQVMHWLRTAARLPDPSMDEQYAVIAEQYEAMLEHY
                     GPVIGANCARKHIGWYTRGLHGSAEFRNAFNKEPDPVRARAMLREFYQPWLTRAAA"
     gene            4893..6059
                     /locus_tag="VF589_06565"
     CDS             4893..6059
                     /locus_tag="VF589_06565"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX8257278.1"
                     /translation="MGSTGRTIALLVAAGSGSRVGGGLPKQYALIAGKPLLAYALDGL
                     LHPAIHDVQVVIAPGQEALYAAAAGDRPLPPPVIGGATRQQSVRNGLEAIASAGGVDR
                     VLIHDAARPFLPVRVIEALLARLEEADGAVPVLAVVDSLARAGGGLGETVPREGLVRV
                     QTPQAFRFDRIIEVHRRWAGELEATDDAQVARAAGIEVALVEGDSALEKLTYAEDFAR
                     AEERLAARMVSRTGLGFDVHAFAEGEQLWLGGLSIPHPRGLRGHSDADVVLHALTDAL
                     LGAIGAGDIGDHFPPSDPQWRGAASSIFLDHVRALIDARGGRIDHADVTIICEAPRIG
                     PHRDAMRARIADLLRVPAARISIKATTTERLGFTGRGEGIAAQAIATVRLPEET"
     gene            6056..6565
                     /locus_tag="VF589_06570"
     CDS             6056..6565
                     /locus_tag="VF589_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257279.1"
                     /translation="MIHTALAAALLVAQVSPPAAKPCLNRAEAADLALLMLPGFVDGL
                     AKTCGPRLPQTAFLRTGSGALSARIRSESAAGLPRALAAFAKTGDEPMPPGVSAATMR
                     QLVDEVAAGLADSTVKPQDCAAADEMIGALSPLPAANLGNVVAVLMQLGAKSDGPNGS
                     GLRMCAAAR"
     gene            6562..7089
                     /locus_tag="VF589_06575"
     CDS             6562..7089
                     /locus_tag="VF589_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX8257280.1"
                     /translation="MSERLDFTLPPQLVELAARVIEANRARGKRIAVAESCTGGLVAA
                     ALTETPGSSDVFEAGFVTYSDEAKVDSLGVSLDVLETFGAVSVAVAWAMAQGALKRTQ
                     ADTTVAVTGIAGPEGGSERKPVGTVVFARARRGADPKEMVADRKDFGDLGRGGVRLQA
                     ALCALELLMPVEPWP"
     gene            complement(7035..7511)
                     /locus_tag="VF589_06580"
     CDS             complement(7035..7511)
                     /locus_tag="VF589_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX8257281.1"
                     /translation="MPRHTETRNLPYTPDQMFELVADVKRYQEFLPWVAATRVRSDSD
                     TLMIADLVVGFRSLKETFTSRVTKHRPTEIVVDYIEGPLKYLHNSWTFHPDGSGGTDI
                     HFCVDFAFKSRIFETLAGQMFDRALRRMIGAFETRAHELYGQGSTGISSSSAQSAA"
     gene            complement(7519..8442)
                     /gene="lipA"
                     /locus_tag="VF589_06585"
     CDS             complement(7519..8442)
                     /gene="lipA"
                     /locus_tag="VF589_06585"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX8257282.1"
                     /translation="MSEVIAPLIAPRQRKPDWIRVKAPTSVGFAETKKLMRRLNLATV
                     CEEAACPNIGECWTKKHATVMILGDTCTRACAFCNVKTGMPRAVDPLEPEHVAVAAAE
                     LGLTHIVVTSVDRDDLPDGGGRQFVKVIEALRRNTPSTTIEILTPDFRNKAQSAVEAI
                     VEAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLESVKRHDPKIFTKSGVMVGLGEE
                     RLEVHQVMDDMRSADIDFLTMGQYLQPTPRHAKVAEFVTPQAFSAYAAIARAKGFLLV
                     AASPLTRSSYHAGDDFLKMRAAREAQLAAAR"
     gene            8536..9171
                     /locus_tag="VF589_06590"
     CDS             8536..9171
                     /locus_tag="VF589_06590"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX8257283.1"
                     /translation="MADFNALLDGYRRFRETGWSSQRDRWSRLAEGQAPKVMVIACSD
                     SRVEPALIFDASPGEMFVVRNVANLVPPFETGGGRHGISAALEFAVTQLCVEEVIVLG
                     HGFCGGCAAALTGQFAGAGHGQGGFIARWLELLDEARDQIRANHAELDRAAFRAMELE
                     AVRVSLANLRTFAWVADRESAGSLTLHGAYFAIADGVLHLLDEATGGFSPA"
     gene            complement(9532..9753)
                     /locus_tag="VF589_06595"
     CDS             complement(9532..9753)
                     /locus_tag="VF589_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257284.1"
                     /translation="MLAHPYRFDAALRGYHAVYHLGEVNHCPGCGRTHWYVGRLSAEC
                     GFCSTALPLADTGMTGTGLLRRHHWVEAA"
     gene            complement(10046..>11029)
                     /gene="gatB"
                     /locus_tag="VF589_06600"
     CDS             complement(10046..>11029)
                     /gene="gatB"
                     /locus_tag="VF589_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX8257285.1"
                     /translation="PDMRSPDEAGAYVRKLRSILRYVGSCDGNMEQGSMRADVNVSVR
                     RPGAELGTRTETKNVNSVRFIMAAIEYEAHRQVDILEGGGEIVQETRLFDPDRAETRS
                     LRSKEDAHDYRYFPDPDLLPLELSPAFLDECRASLPELPDAKRARYEAELGLSPYNAA
                     VLTAEADTARWFEALLAEGALKQGRPATQLAKAAANWLISDLFGALNRLGRTIEDSPV
                     TPAQGAELLALVADGTLSGTLAKQVFELMLETGEDPAAIVEARGLKQTSDTGAIEAAI
                     AAVMASNADKVADYRGGKDKLFGFFVGQVMKAMAGKANPQVVNELLKKALG"
BASE COUNT         1681 a         3661 c         3867 g         1820 t
ORIGIN      
        1 tgttcttggc cccggacgga agccggtccg gagctggcgc ggcgcctcaa tatcatggcg
       61 agggccgcca tgctatcggc gcttactccc cctcttctcc cttggtgtcg ccgatcccca
      121 gcgccttgag cttgcggtgc agcgccgacc gctccatccc gatgaaagtc gcggtacggg
      181 aaatgttgcc ggagaaacgc cggatctgga ccctcagata ttcgcgctcg aaggtctcgc
      241 gcgcttcccg gagcggagtg cccatgatcg ccttgaccgc ttcacccggg ctcagttgcg
      301 cctgctcgtt cagcagctcg ggcggcagca tgtcgatttc gatgcaggcg acccgcgagc
      361 tcggcgcgag gatgatcgtg cgctcgatga cgttcctgag ctgcctgacg ttgccgggcc
      421 agtcataggc ctggagcgcc gcgaccgcgt cgtcggacag ggtgggcgtt ggcaaccttt
      481 gctcggcagc gtagcgggcg gcgaaatggg tcaccaattc gggaatgtcc tcgcgccgct
      541 cggccagcgc cggcaagtgg accggcacga cgtttagccg gtagaagagg tcctcgcgga
      601 atcgcccgcc ggcaatttca tccgccagga tccgcgccgt cgacgagacc acgcgcatgt
      661 cgaccctcac cgtgcgttgg ccgcccaccc gcgtgaagct ctggtcggtg aggactcgca
      721 ggatcttggc ctgggccggg atcggcatgt cggcgatctc gtcgaggaag agcgtgccgc
      781 catgcgcctg ttcgagcagg ccgggccggg ccagctcgcc cccctcctcc accccgaaca
      841 gttcctcctc gacccgctcg ggagtcatcc gcgccgcgct gaccacgatg aacggcgcct
      901 tggcgcgcgg gctccaattg tggagcaagc gggcggcgac ctccttgccg acgccggccg
      961 gcccggtgat gagcacccgg ctgccggtcc ccgccacccg cttcagggtc gcgcggacgg
     1021 cgttgatcgc gacgctcgac ccggtgagct cggtctcctg tccgatctgc tggcgcaggc
     1081 tctcattctc gcgccgcagc cggtcggtct cggtcgctct tgcaacgagg tgcagcaagt
     1141 ggccggcttc gaacggtttt tcaatgaagt cgacggcacc ctgacgaatg gcggcgacgg
     1201 cggtgtcgag attgccatgg cccgaaatca tcagcaccgg cagcgaaggg tcgcggcgct
     1261 tcacttcctc gagcagttgc aggccatcga gcttcgagcc ctgcagccag acgtcgagca
     1321 gcaccagcga cggccggcga tcggccaggg ccgcgagcgc ctcgctgctg ttggccgccg
     1381 tcctggcttc atagccctca tcttccaaaa cccccgagac gagctcccgg atatcctgtt
     1441 cgtcgtcgac tacgagaatg tctaatgcca ttgggtcaac cgttcatcgc ctgatcttcg
     1501 gaatgatatt cgccgctccc ccccaggcgc gcgagcgttt cggcgtcgaa gatcagccgg
     1561 accaaagtcc cgccgcccgg tgcgtcgtcg aactcgatga cgccggaatg ttcttcgacg
     1621 atcttcttga cgatggcgag cccgaggccc gtgcccttga cccgcgtcgt catgtacggc
     1681 tcggtcagcc ggccgcggtc ggccgggagc ccgacaccgg tatcgctgat ctccagcgtc
     1741 acgcgccccg gcgcttcgac gatccggatc tcgatccggt cgctatcggc gtgcccccgc
     1801 tcctcattct tctgctggat cgcctcgacc ccgttcttga cgatgttggt caaggcctgg
     1861 ccgagctggc ggcggtcgca gaccagcgcc ggcgacggct ccggcccatc gaggacgaag
     1921 cggatttcgg gatgcgcgac ctcgtgcagg aacagagcct ggcgcgcgat gtcggcgata
     1981 tcctcgtcgc ggaaaacggg cttcggcatc cgcgcgaagg acgagaattc gtcgaccatc
     2041 cgcctgaggt cgccgacttg gcgaacgatg gtcccggtca gccgctcgaa ggtcgctccg
     2101 tcggagctga tctcccgccc gtatcgccgc tgcagccgct cggcggcgag ctggatcggg
     2161 gtcagcgggt tcttgatctc gtgcgcgatc cgctgcgcga cgtcggacca ggcggcgcgg
     2221 cgctggtcga gcagctgctc ggtgatgtcg tcgaaggtga gcacatggcc cccttccacc
     2281 ttgacgcgct tgacggcgag cgtgcgcggc tcgccgcccg aggagaattg gatgatgccc
     2341 tcgcgctcct cgctgtcgag ctgccggtcg agctcgggcg cgagcacggc cagcttctgg
     2401 ccgaccgcgc tcttgttgcc ggtcttgagc aaggcttcgg cggagctgtt gatgagccgc
     2461 acctcgcggt tggtgtcgac cgacaggatg ccggcggtga cgcctgacag caccgcctcg
     2521 atgaaggcgc ggcggctgtc gagctgattg ttggcgctga ccagcgcgcc ggtctgctcc
     2581 tcgaggcggc gggtcatccg gttgaatgcg ttgccgagcg tcgagacctc gtcattgacc
     2641 cgagacgtgg gcacccgcgc cgacaggtcg ccggcggtga cgcgccgcgc cgcatccacc
     2701 agctcgccca cgggccgcac cagccggtcg gccagcgtca gcgcgatcca gatcgagagc
     2761 gccacgatca gcagcgagac cagcagcagc accgcgttga agcggaactg gagggtgcgc
     2821 gagttcagca gggtcttctg ataatcgctc gccgcgctcc gcgcttcctc gatctgctgg
     2881 atcgccgccg gattcacggt gcgcgaggtg tagagataga cctcctcttc cggatcgagg
     2941 cggacgaccg cctcaacgcg gtcgccggcg tcggtgacga cgcgcgtctc ccctcccctc
     3001 agctcgctga gcaccggcgc cgggaagcgc tgttcgagcg gacggtcgtc gagattgacg
     3061 ccggcgcgca gctggaggtc gccggtccgg gaaaccgaga tcaccgccgc ctcggtaagg
     3121 tcgcggtaga cggtctggag aaacagcttt tcggcaaacg ccggcgtgtc gaggccgaac
     3181 tggttgatgt ccgccacgat atccaccgtc atcgcctgga cgtcgcgata gatgcggctg
     3241 cgattctcct cggcatagac ctgcgcgaca ttgtcggcat tttcgaggat ggtgcgcgcg
     3301 cggtcggaga accagaattg caccccctgc tgaaacagca gcgaggcgaa gatcaccacc
     3361 atcagcgtcg gcacgctggc gatcgccgag aacaaggcga ccaggcgcac gtgcagccgg
     3421 ccgcgcccgc ccatcggcga gcgcgcggcc cggcccatcg ccacccgccg ggcgatcagc
     3481 accatcagcg ccatcgccgg caccaggttg gcgaccagca gcatcgccac catcggcggc
     3541 gtcagcagcg actgcggcga gccctgctcg gcgacgatga aatagctgaa cgtcagggtc
     3601 gcgatcagcg ccagcacggt cgcaatctcg accatcggca tgatcgggct ccgccgcagc
     3661 acgatcgaaa gccgccggcg caggcggctt ctcgaatcaa caatgggcat gggcgcttcg
     3721 gcgttcacgt ggccgctcta ccacgagaat gtgacctaaa gaacacacat ctgactcaat
     3781 tgagcgcgcc gcgcgcggcg ttcggctcag gcagggacgg aaggccgctc aggagcaagt
     3841 caggcggcgg ctcgcgtcag ccacggctga tagaattcgc gaagcatcgc ccgtgcccgc
     3901 accggatcgg gttccttgtt gaaggcgttg cggaactccg ccgagccgtg caggccgcgc
     3961 gtgtaccagc cgatatgctt gcgcgcgcaa ttggcgccga tcaccgggcc gtaatgctcc
     4021 agcatcgcct catattgctc ggcgatgacg gcatattgtt cgtccatgct tgggtccggg
     4081 agccgcgctg cggtgcgcag ccagtgcatc acctgcccga gcagccacgg cttgccatag
     4141 gcgccgcggc cgatcatcag cccgtccgcg cccgattggt cgagcgccgt ctcggcatcc
     4201 tcgatcgaat tgatgtcgcc gttgacgatc accggcaccg acaccgcctc cttgacccgc
     4261 cgaacgaagc gccagtccgc ctcgccctta tagagctggc agcgggtgcg gccgtggacg
     4321 gtgatcatcc tgacgccgag gtcctcggcg atgcgggcga gctccggcgc gttgaggctg
     4381 ccatggtccc agcccatccg catcttgagc gtgaccggga ccttcaccgc tccgaccgtc
     4441 gccttgatga tcgcggtggc gagcgggatc tgccgcatca gcgccgagcc ggcgtcgccg
     4501 ttcaccacct tcttgaccgg gcagcccatg ttgatgtcga tgatcgcggc gccgcgatcc
     4561 tcgttcagct tggcggcctc ggccatctcg gcgggagagc agccggcgag ctgcatcgag
     4621 accggctcct ccaccgggtc ccacgcggct ttctgcagtg actggcgcgt ttcgcggatc
     4681 atcgcctgac tggcaatcat ctcggtgacg gtgagcccgg ccccgtagcg cttgacgatc
     4741 cgccggaacg gccggtcggt gacgccggtc atcggcgcca ggatgacggg cgcttcgatc
     4801 cggaccgggc cgatcgcaat gggagcaagg cgggacatga ggccacggca cataatgttg
     4861 atgccgctgg cgggcaaggt taggggcagc ctatgggatc gaccggacgg acaatagcgc
     4921 tgctggtcgc ggcagggagc gggagcaggg tcgggggcgg cctgcccaaa caatatgccc
     4981 tgatcgccgg caagccgctc ctcgcgtacg cgctcgacgg cctcctccat cccgccatcc
     5041 acgacgtgca agtggtcatc gcccccgggc aggaggcgct ttatgcggcg gcggccggcg
     5101 accgccccct gcccccgccc gtgatcggcg gagctacgcg ccagcaatcg gtgcgcaacg
     5161 gccttgaggc gatcgcctcg gcagggggcg tcgacagggt gttgatccac gacgcggccc
     5221 gtccgttcct ccccgtgcgc gtgatcgagg cgctgctggc gcggctggag gaagcggacg
     5281 gagcggtgcc ggtgcttgcc gtcgtcgaca gcctcgcacg cgccggcggc ggcctaggcg
     5341 agacggtgcc gcgggagggg ctggtccgtg tccagacgcc ccaggccttt cgcttcgatc
     5401 gcatcatcga ggtgcatcgg cgctgggccg gcgaattgga agcaaccgac gacgcccagg
     5461 tcgcgcgggc cgcgggcatc gaggtcgcgc tcgtagaggg ggattccgcc ttggagaaac
     5521 tgacttacgc cgaggatttc gcccgtgcgg aggagcggct cgccgcccgc atggtctcgc
     5581 gcaccggcct cggcttcgac gtccacgcct ttgccgaggg tgagcaattg tggctgggcg
     5641 gcctctccat cccccacccc cgcggcctca ggggccatag cgacgccgac gtcgtgcttc
     5701 acgcgctcac cgacgctttg ctcggcgcga tcggcgcggg cgacattggc gatcacttcc
     5761 cgccgagcga cccgcaatgg cgcggcgccg cttcgtcgat cttcctcgac catgtccgcg
     5821 ccctcatcga cgcgcgcggc ggcaggatcg accatgccga cgtcaccatc atctgcgagg
     5881 cgccgcggat cggcccgcat cgggacgcca tgcgggcgcg gatcgctgac ctgttgcggg
     5941 tgccggccgc gcggattagc atcaaggcga cgaccaccga acggctcggc ttcaccggac
     6001 gcggcgaagg catagcggcg caggcgatcg ctaccgtcag gctcccggag gaaacatgat
     6061 ccacaccgct ctcgccgccg ccctgctggt cgcgcaagtc tcgccgccgg cggccaagcc
     6121 ctgcctcaac cgggcggagg cggcggacct cgcgctgctc atgttgcccg ggttcgtcga
     6181 cggcctcgcc aagacgtgcg gaccgaggct gccgcagacc gccttcctcc gcaccggctc
     6241 gggcgcgctc tccgcccgga tcaggagtga gagcgccgct ggattgccgc gagccctggc
     6301 tgcgttcgcg aagaccgggg atgagccgat gccgccgggg gtgagcgccg cgaccatgcg
     6361 gcagctggtc gacgaggtcg ccgccggctt ggcggactcg acggtcaagc cccaggattg
     6421 cgccgcggcc gacgagatga tcggagcgct gtcgcccttg cccgccgcca atctcggcaa
     6481 cgtggtggcc gtgctgatgc agctcggcgc gaaatcggat gggcccaacg gttccggcct
     6541 tcgcatgtgc gcggccgccc ggtgagcgag cggctggact tcacgctgcc gccgcagctg
     6601 gtcgagctgg ccgccagggt catcgaagcc aatcgcgcgc gcggcaagcg gatcgcggtg
     6661 gccgagagct gcaccggcgg gctggtggcc gcggcgctga ccgagacgcc gggctcgtcc
     6721 gacgtgttcg aggctgggtt cgtcacctat tcggacgagg ccaaggtcga cagcctgggc
     6781 gtcagcctcg acgtgctcga gacgttcggg gcggtgtcgg tggcggtggc ctgggcgatg
     6841 gcgcaaggcg cgctgaagcg cacccaggcg gacacgacgg tggcggtgac cggcattgcc
     6901 gggcccgagg gcggcagcga gcgcaagccg gtcggcaccg tggtcttcgc ccgcgcccgg
     6961 cgcggcgccg accccaagga gatggtcgcg gaccgcaagg atttcggcga cctcggccgc
     7021 ggcggcgtcc gccttcaggc ggcgctttgc gcgctggaac tgctgatgcc ggtcgagccc
     7081 tggccgtaaa gctcgtgcgc gcgcgtttcg aaggcgccga tcatccgccg cagggcgcgg
     7141 tcgaacatct ggccggcgag cgtttcgaag atgcgcgact tgaacgcgaa gtccacgcag
     7201 aaatggatgt ccgtgccgcc cgagccgtca gggtggaagg tccagctgtt atgcaaatat
     7261 ttgagcggcc cctcgatata gtcgacgacg atctcggtcg ggcggtgctt ggtgacgcgg
     7321 gaggtgaagg tctccttcag cgagcggaag ccgacgacga ggtcggcgat catgagcgtg
     7381 tcgctgtccg accggacccg cgtcgccgcc acccagggga ggaattcctg gtaccgcttc
     7441 acatcggcga ccagctcgaa catctggtcc ggcgtgtagg gcaggttccg agtctcggtg
     7501 tggcggggca tcgcggcttt aacgcgcggc ggcgagctgg gcttcgcggg cggcgcgcat
     7561 tttcaggaaa tcgtcgccgg catggtagct ggagcgcgtc agcggcgagg cagcgacgag
     7621 caggaagccc ttggcgcggg cgatcgcggc ataagccgaa aaggcctgcg gcgtgacgaa
     7681 ctcggcgacc ttggcgtggc gcggggtggg ctggagatat tggcccatgg tgaggaaatc
     7741 gatgtcggcc gagcgcatgt cgtccatcac ctggtggacc tcgagccgct cctcgccgag
     7801 gccgaccatc acgccggact tggtgaagat cttcggatcg tgccgcttga cgctctcgag
     7861 caggcggagc gaggcgtagt agcgcgcgcc cgggcggatg gtgggataga gccgcgggac
     7921 ggtctcgaga ttatggttat agacgtcggg tcccgcctcg acgatcgcct cgaccgccga
     7981 ctgagccttg ttgcggaaat cgggagtgag gatctcgatg gtggtggacg gcgtgttgcg
     8041 gcgcagcgcc tcgatcacct tgacgaactg gcggccgccg ccgtcgggaa ggtcgtcgcg
     8101 gtcgaccgag gtgacgacga tatgggtgag gccgagctcg gcggcggcga ccgcgacatg
     8161 ctcgggctcg agcggatcga ccgcgcgcgg catgccggtc ttgacgttgc agaaggcgca
     8221 ggcgcgggtg caggtgtcgc cgaggatcat cacggtcgcg tgcttcttgg tccagcactc
     8281 gccgatattc gggcaggccg cttcctcgca gacggtggcg aggttgaggc gccgcatcag
     8341 cttcttggtt tcggcgaagc cgacgctggt cggcgccttg acgcggatcc agtcgggctt
     8401 gcgctggcgc ggcgcgatga ggggagcgat gacttcggac atggggggca gatagcgatc
     8461 tgaaagccca cttgccaccc ctcccctcgc ctggctagca gcggcggcga cgcttctcgt
     8521 ggggggtgaa acaggatggc cgatttcaac gcgctgctgg acggctatcg ccgcttccgc
     8581 gagactggct ggtcgagcca gcgcgaccgc tggtcccggc tcgccgaggg ccaggcgccg
     8641 aaggtgatgg tgatcgcctg ctcggacagc cgggtcgagc cggcgttgat ctttgacgcc
     8701 agccccggcg agatgttcgt ggtccggaac gtcgccaatc tggtgccgcc gttcgagacc
     8761 ggcggcggcc gccacggcat ttcggccgcg ctcgaattcg cggtcactca gctctgcgtc
     8821 gaggaagtga tcgtgctcgg ccacggcttt tgcggcggct gcgcggcggc gctgaccggg
     8881 cagttcgcgg gcgcaggcca tggccagggc ggcttcatcg cccgctggct cgagctgctc
     8941 gacgaggccc gcgaccagat ccgcgccaac catgccgagc tcgaccgggc ggcgttccgg
     9001 gcgatggagc tggaggcggt ccgggtcagc ctcgccaatc tcaggacctt tgcctgggtg
     9061 gcggatcgcg agtcggccgg ctcgctcacc ctccacggcg cctatttcgc gatcgccgac
     9121 ggcgtcctcc atctgctcga cgaggcgacg ggggggttca gcccggccta ggcagggagg
     9181 gtggtgaact tgacgaactt gtcactgtgg aggggtgttg gccggctcgg aaggtgacta
     9241 acttgtcgaa cctgtcgctg cggaggggtg ttcgcctgct tggaaggtta cgaacttgtc
     9301 gaacctgtcg ctgtgagact gtgttcgcct ggcgcaagtt gatcaggttg cggcggcctg
     9361 cattggctgg accccggcct tcgccgggga acatccggca gggtgcggcg aggatcaacg
     9421 gtgcgaaaga gccggcgtgg ccccgcaggc ggcgccaccc ggcacgctgt caggcgaaat
     9481 cctacattgc aatccccgcg gggtcggccc gccacccccg gccgaacggc gtcaggccgc
     9541 ttcgacccaa tggtggcggc ggagcaggcc ggtgccggtc atgccggtgt cggcgagcgg
     9601 aagcgcggtg ctgcaaaagc cgcattcggc cgacagccgg ccgacatacc agtgggtccg
     9661 cccgcagccg gggcaatggt tcacctcgcc gagatgatag accgcatgat agccgcggag
     9721 cgccgcatcg aaacgataag ggtgcgcaag catctggacc tcgcttcggg cctcgccgcc
     9781 ttcggctcgg ccccttccct ccctctatag cagtaggacg ggttgcgacg aagcgttgtt
     9841 tatcgtcggt gaaacgattg cccattaacg ttgcggcggc gcaacagagg catcccggcg
     9901 gccaccaccg tcatcccagc gaaagctggg atctcccttc ccctggcgat gccgacccgg
     9961 gccggcgacc gaaaggcaaa gagatttcca gcttgcgatg gaatgacgaa ttacgctgga
    10021 gtgacgaagc tgcgccgcga cggcgtcagc ccagcgcctt cttcaagagc tcgttgacga
    10081 cctgcgggtt ggccttgccg gccatggctt tcatcacctg gccgacgaag aagccgaaca
    10141 atttgtcctt gccgccgcga tagtcggcga ccttgtcggc gtttgacgcc atgacggcgg
    10201 cgatggcggc ttcgatcgcg ccggtgtcgc tggtctgctt gaggccgcgc gcctcgacga
    10261 tggcggcggg atcctcgccg gtctcgagca tcagctcgaa cacctgcttg gcaagcgtgc
    10321 ccgacagcgt gccgtcggcg accagggcca gcaattcggc gccctgggcc ggggtgaccg
    10381 gactgtcctc gatcgtccgg ccgagccggt tcaaggcgcc gaacaggtcc gagatcagcc
    10441 agttggcggc ggccttggcg agctgggtgg cggggcggcc ctgcttcagc gcgccttcgg
    10501 cgagcagagc ctcgaaccag cgggccgtgt cggcctcggc ggtgaggacg gcggcattgt
    10561 agggcgacag gccgagctcg gcctcgtagc gggcgcgctt ggcgtcgggc agctcgggca
    10621 ggctggcgcg gcattcgtcg aggaaggccg gcgacagttc gagcgggagc agatcgggat
    10681 cggggaaata gcgataatcg tgcgcatcct ccttggagcg caggctgcgc gtctcggccc
    10741 ggtcggggtc gaacaggcgg gtctcctgga cgatctcgcc gccgccttcg aggatatcga
    10801 cctggcggtg ggcctcatat tcgatcgcgg ccatgatgaa gcgcaccgaa ttgacgttct
    10861 tggtctcggt gcgggtgccg agttcggcgc cggggcggcg gacgctgaca ttgacgtcgg
    10921 cgcgcatcga gccctgctcc atattgccgt cgcacgagcc gacatagcgc aggatcgacc
    10981 ttaatttgcg gacgtaagcc ccggcttcgt cgggcgaacg catgtcggg
//
LOCUS       DASVRK010000170         8046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_281301, whole genome shotgun sequence.
ACCESSION   DASVRK010000170 DASVRK010000000
VERSION     DASVRK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281301"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..629)
                     /locus_tag="VF589_06605"
     CDS             complement(288..629)
                     /locus_tag="VF589_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257286.1"
                     /translation="MGSYADVDPVIESWVKATGSSLFTEWADVQARFFHLPGDPPFEC
                     FQISVRLPQNGQTAVTARAIDTNDHSDEGMDRTWEGPISELDSMLRAAVGTVKSWKAR
                     RRKMPDPPSPQ"
     gene            complement(930..1595)
                     /gene="purQ"
                     /locus_tag="VF589_06610"
     CDS             complement(930..1595)
                     /gene="purQ"
                     /locus_tag="VF589_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX8257287.1"
                     /translation="MKSAVIVFPGSNCDRDLAVALEQVTGTKPAMVWHRDTELPDGLD
                     LIGVPGGFSYGDYLRSGAMAARSPIMRAVKDAADRGVPVLGICNGFQVLTEAGMLPGA
                     LMRNSSIAFVCRDVALKVENSQSVFTSRYDAGEEIVVPVAHHDGNYQADPETLERLEG
                     EGRIAFRYSGEVNGSARDIAGIINESGNVLGMMPHPERMIEPAHGGTDGRRLFEGLLE
                     AVG"
     gene            complement(1606..1845)
                     /gene="purS"
                     /locus_tag="VF589_06615"
     CDS             complement(1606..1845)
                     /gene="purS"
                     /locus_tag="VF589_06615"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX8257288.1"
                     /translation="MKTRVYITLKTGVLDPQGKAIHHALEGLGFAGVNGVRAGKLIEL
                     DLEDSVSEADIEAMCRKLLANTVIENFRIERPAAA"
     gene            1957..2292
                     /locus_tag="VF589_06620"
     CDS             1957..2292
                     /locus_tag="VF589_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX8257289.1"
                     /translation="MSETPDISLTPSAAARVAAIAGRQGKPAILRLAVDGGGCAGFQY
                     RFGLADTVEAGDTQAIEGGVTLVVDEVSLDLVRGSAVDFVESLGGASFQVTNPNAASG
                     CGCGTSFSI"
     gene            2413..3057
                     /locus_tag="VF589_06625"
     CDS             2413..3057
                     /locus_tag="VF589_06625"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8257290.1"
                     /translation="MKRSLLFAAAAGVLLNSANLPASPPPLLLPIPENLAEMEVDWTA
                     AEPDFAPELQPAPPLPAAPPAPPVAAAPEPPPAAPPPPPPPDPDNGVRIVISLPLQKA
                     YVFAGGELVDTTPVSTGKRGYETPAGTFPILQKKVHHRSNKYSNAPMPYMQRLTWGGV
                     ALHAGSLPGYPASHGCIRLPRSFAKKLYGLTDFNTLVTITREKPESAEDALALI"
     gene            3119..3898
                     /gene="xth"
                     /locus_tag="VF589_06630"
     CDS             3119..3898
                     /gene="xth"
                     /locus_tag="VF589_06630"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8257291.1"
                     /translation="MKIVTYNLNGIRARLPRLLEYLDEQKPDVVCLQEIKCADEAFPA
                     KDLEGAGYGLVHHGQKGFNGVAILVRGAQPQLRRMGLPGDPDDTHSRYIEVDVGGLVV
                     ASIYLPNGNPVGTEKFQYKLAWMERLRAHSAELLAEERPTVLAGDWNVIPTDDPDDVF
                     SVRAMAHDALMQPESRQAYRRILHHGWTDAIRARHPDGAVYTFWDYTAGSWQRDAGFR
                     IDHLLLSPQAADRLLDAGVDKDYRGREKASDHAPTWVELEG"
     gene            3973..6297
                     /locus_tag="VF589_06635"
     CDS             3973..6297
                     /locus_tag="VF589_06635"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8257292.1"
                     /translation="MNFVTTWWGRVERPLQKSGAGRLPVILCAALVGVVAGLGQFGSP
                     VEDMLRSARNLARSHDASGQVVIIALDDKSLTGVQKWPWPRRYHAKLVDNLTAMGARR
                     IFFDIEFSSNSNAKDDAILAGALARSKTEVTLANLLAFDPVTGTQTNTFPIPEFRRHA
                     RQATINLGYEEDGSVWKIPYGVKMDGTFYPSFAASLAGSSIQEEQYFRLDHAIRPESI
                     PSVSAIDVLDKSAPVASIAGKDVVIGAASMQLADIFNAPGHGLISGVYLHAIAAETLM
                     KGRPTELSWLLMLSLAALMMTLASSFLRGFRMLAGYCVVPIAILAGATILEHFLIVPA
                     VLPALLTSAIGILIQSVLLLRQRMAERASVNPISGLPNLKAFRDTAPFVTDYVIVARV
                     HNFTKIVASLPDDCEKELVRQIAARLSVGRGDSPVFQSDDGTFITLFARSDSVEDHAS
                     ALHNLFREPVTFGPTRFDLIVTLGVDGEVERPLTRRLADALNAADEAFGKGLKWKMAV
                     AEGVEDTEWHLSLLGQLDEAIERKHMWVAYQPKLDLASGRLCGAEALVRWAHPKRGNI
                     SPIEFIQAAEQSGRIDRLTAFVMDDAIGAAAALAARGTFIDMSVNLSARMLGDDGLVA
                     MVSGLLHTHRFPPERLILEITETAAIDASRLAQLTRLRELGVGLSIDDYGTGLSTLEY
                     LKCIPATEIKIDGSFVQNLSTSMSDRLMVSSTINLAHALGRRVVAEGVEDPASLEALS
                     TMQCDEAQGYYIGRPMPLRQLYRALARHERRQAA"
     gene            7587..>8046
                     /locus_tag="VF589_06640"
     CDS             7587..>8046
                     /locus_tag="VF589_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257293.1"
                     /translation="MINKTILATACAAIALAVTATPAAAKWVNGVKVEDNLPRHSGDD
                     DMVNGVKVRGDGSVDDNGPRRNRGGRGGEDSVDSDDDDDNDGRGRNRRGRGSDDGVDS
                     VDGDDDGGRGRGRGRGRGSDDSVDSVDGDDDGGRGRGRGRGRGGDSVDSVD"
BASE COUNT         1301 a         2674 c         2695 g         1376 t
ORIGIN      
        1 agacgagacg gtattctttg tttctttcaa atgtgcggcc aggccgaagc gtgacgagta
       61 tagctacttt agcatcgtcc tcgaaaatag ccgtcctgtg cgtgtttata tgcagcggtc
      121 atacctggtg cccgtgttcg agtcggctcc tactggggag agcccatgac gcaagtcggg
      181 cggcagcgct gtcggctccc gccggtcgtc tggctccggg tgaattggag cggctgatgc
      241 tcggcggtgt ttgaccgata aatgtccgcg atcggtggaa agcgggccta ttgaggtgag
      301 ggcgggtcgg gcatctttcg ccggcgagcc ttccaggatt taacggtccc caccgcggcg
      361 cggagcatgc tgtcgagttc ggaaataggc ccttcccaag tccggtccat tccctcgtcg
      421 ctatggtcgt tggtatcgat agcccgtgcc gtgacggccg tttgcccgtt ttgcggcaac
      481 cgcacgctga tctggaagca ttcgaacggg ggatcgcccg gaaggtggaa aaatctcgcc
      541 tggacatccg cccattcggt gaacagcgac gatccggtcg ccttcaccca gctctcgatc
      601 acaggatcca catccgcata gctgcccatc ggcgcttgat gccgcgcggg cgggatgtcc
      661 gcaatgggat gaaacgaggc agtcgcctca actccccgcc tgcgctaaga ggcaggcatg
      721 acgaggtccg cggcggtcga acgtgctttt ggtcttgccc ggtcggggca gtgccgcagc
      781 gtcgcggaga ttatccggcg gctgcccgaa ggcgatcggc cggcggtcga agagcatttg
      841 agggcgccgt cgaccaggcg ggaaatgatc ctggtctgca gcgacgcctg gctcgccgct
      901 cgatagcggc gggacgcgat tgcggcatct cagccgaccg cttcgagcag cccctcgaac
      961 aggcggcggc cgtcggtgcc gccatgagcg ggctcgatca tgcgctcggg gtgcggcatc
     1021 atgccgagta cgttgccgct ttcgttgatg atgccggcaa tgtcgcgcgc cgagccgttg
     1081 acctcgcccg aatagcggaa ggcgatgcgg ccttcgcctt cgagccgctc gagcgtttcc
     1141 gggtcggcct ggtaattgcc gtcatgatgc gcgaccggga cgacgatctc ctcgccggca
     1201 tcgtagcggc tggtgaagac ggactggctg ttctcgacct tgagcgcgac atcgcggcag
     1261 acgaaggcga tggacgagtt gcgcatcagc gcgccgggca gcatgccggc ctcggtcagc
     1321 acctggaagc cgttgcagat gccgagcacg gggacgccgc ggtcggccgc gtccttgacc
     1381 gcgcgcatga tcggagagcg cgccgccatc gcgcccgagc gcagatagtc gccgtaggag
     1441 aagccgccgg ggacgccgat caggtcgagc ccgtcgggaa gctcggtgtc gcggtgccag
     1501 accatggccg gtttggtgcc ggtcacctgc tccagcgcga cggcgaggtc gcggtcgcaa
     1561 ttggagccgg ggaagacgat gacggcgctt ttcacgtgtc cctcctcagg cggcggcggg
     1621 ccgctcgatc cgaaaattct cgatgacggt gttggccagc aatttgcggc acatggcttc
     1681 gatgtcggcc tcgctgacgc tgtcttcaag gtcgagttcg atcagcttgc cggcgcggac
     1741 gccgttgacg ccggcaaagc cgagcccttc gagcgcgtgg tggatcgcct tgccctgcgg
     1801 atcgaggacg ccggtcttga gcgtgatgta gacgcgggtt ttcatggcgg ccgctctatg
     1861 gcgagaggcg cggcggggca agggcggagc ggcggcggtt gggcgggaaa ggggacgatg
     1921 cttgccgcgc ccggcgccgc cccctatctc atgcgtatga gcgagacccc tgacatcagc
     1981 ctgacgccct cggccgcggc ccgggtggcg gcgatcgccg ggcgccaggg caagccggcg
     2041 attctgcggc tcgccgtcga cggcggcggc tgcgcgggct tccagtatcg gttcgggttg
     2101 gccgatacgg tcgaggcggg cgacacccag gcaatcgagg gcggcgtgac tttggtggtc
     2161 gacgaggtca gcctcgatct ggtgcgcggc agcgcggtcg atttcgtcga atcgctcggc
     2221 ggcgcgtcct tccaggtcac caaccccaac gccgcttccg gctgcggctg cgggaccagc
     2281 ttctcgatct gacgctccgg ccggggggcc tgggagacaa ggccgccagt tattctggcg
     2341 gtccgggagc aagttggtta atggatcgtt tcggttccgc cggggagtcg caggctcccc
     2401 tcaaggagga agatgaagcg atcgttgctc ttcgccgcgg cggccggggt gctgctcaac
     2461 agcgcgaacc tgccggccag cccgcccccg ctgctgctgc cgatcccgga gaaccttgcc
     2521 gagatggagg tcgactggac cgcggccgag ccggacttcg ctcccgaact tcagcccgct
     2581 ccgccgcttc ccgccgctcc gcccgcgcct ccggttgcgg ccgccccgga accgccgccg
     2641 gccgcgccgc cgccgccgcc tccacccgac ccggataacg gcgttcgcat cgtgatcagc
     2701 ctgccgctcc agaaagccta tgtgttcgcc ggcggcgagc tggtcgacac cacccccgtt
     2761 tccaccggca agcgcggcta tgagactccg gcggggacct tcccgatcct ccagaagaag
     2821 gtccaccacc gctcgaacaa atatagcaac gcgccgatgc cctatatgca gcggctgacc
     2881 tggggcggcg tcgcgctcca cgccggcagc ctgcccggct atccggcctc gcacggctgc
     2941 atccggctgc cgcgcagctt cgcgaagaag ctgtacgggc tgaccgactt caacacgctt
     3001 gtgacgatca cgcgcgagaa gccggaatcg gccgaagacg cgctcgcgct gatctgagcc
     3061 gccgcgaccg cctgagaaca ggcatgacgg gccggggaag ggcggctaag ccggcgccat
     3121 gaagatcgtc acttacaacc tcaacggcat ccgggcgcgg ttgccgcggc tgctggaata
     3181 tctcgacgag cagaagcccg acgtggtctg cctccaggag atcaaatgcg cggacgaggc
     3241 gtttccggcg aaggatctgg agggcgccgg ctacggcctc gtccaccacg gccagaaggg
     3301 cttcaacggc gtcgcgatcc tggtccgggg cgcgcagccg cagcttcgcc ggatggggct
     3361 gccgggcgac ccggacgaca cccacagccg ctatatcgag gtggacgtgg gcgggctcgt
     3421 cgtcgcctcg atctacctgc ccaacggcaa cccggtcggc accgagaaat tccagtacaa
     3481 gctcgcctgg atggagcgtt tgcgcgcaca ctccgcggag ctgctggcgg aggagcggcc
     3541 gacggtgctc gcgggcgact ggaacgtgat cccgaccgac gatcccgacg atgtcttttc
     3601 ggtgcgggcg atggcgcatg acgcgctgat gcagcccgag agccgccagg cctatcgccg
     3661 catcctccac cacggctgga ccgacgcgat ccgcgcgcgc cacccggacg gcgcggtcta
     3721 tactttctgg gactatacgg ccggttcctg gcagcgcgac gcgggctttc gcatcgatca
     3781 cctgctgctc agcccgcagg ccgccgaccg gctgctcgat gccggggtcg acaaggatta
     3841 tcgcgggcgc gagaaagcca gcgaccacgc gccgacctgg gtcgagctgg aaggctgacg
     3901 ccgtggaggg gggacgccct ccgcccccga aacgttacct ttcgcttaac cttttcatgc
     3961 catgctcggc caatgaactt cgtgaccacc tggtggggcc gcgtcgagcg gccgctgcaa
     4021 aagagcggtg ccggccgctt gccggtaatc ctctgcgccg cgttggtcgg cgtggtcgcg
     4081 ggcctcggcc agttcggatc gccggtcgaa gatatgctga gaagcgcccg caacctcgcc
     4141 cgaagccacg acgcctccgg ccaggtcgtg atcatcgcgc tcgacgacaa gagcctcacc
     4201 ggcgtccaga aatggccgtg gccgcggcgc tatcatgcca agctggtcga caacctcacc
     4261 gccatgggcg cgcggcggat cttcttcgac atcgaattct cgtcgaattc aaacgcaaag
     4321 gacgacgcga tcctcgccgg tgccttggcc agatcgaaaa ccgaggtgac tcttgcgaac
     4381 ctgctcgcgt tcgacccggt caccggcacg cagaccaaca cgtttcccat tcccgagttt
     4441 cggcggcacg cacggcaggc gaccataaat ctcgggtatg aagaggatgg gtccgtatgg
     4501 aagattccct atggggtgaa aatggacggg accttctacc cgtccttcgc cgcctccctc
     4561 gcgggctcct ccattcagga ggagcaatat ttccggctcg atcatgccat tcgccccgaa
     4621 agcattccct cggtcagcgc catcgacgtg ctcgacaagt cggctccggt cgcctcgatc
     4681 gcgggcaagg atgtcgtgat tggggccgcc tcgatgcagc tcgccgacat tttcaacgct
     4741 ccgggccacg gcttgatttc gggcgtgtat ctgcacgcca tcgccgctga gaccttgatg
     4801 aaaggccgcc cgacagagct gagctggctg cttatgctgt cgctcgctgc gctcatgatg
     4861 acgctcgcgt cgagcttcct caggggcttc agaatgcttg ccggctattg cgtcgtgccg
     4921 atcgcgatcc tcgcgggcgc aacgattctc gagcatttcc tgatcgtgcc cgcggtgctt
     4981 cccgccttac tgaccagcgc catcggcatc ctcatccagt cggtgctgct gctgcgccag
     5041 cggatggcgg agcgggccag cgtcaatccc atttccggcc tgccgaacct caaggcgttc
     5101 agggacacgg cgccgttcgt gaccgattat gtgatcgtcg cgcgcgtcca caatttcacc
     5161 aagatcgtcg cctccctgcc cgacgattgc gagaaggagc tggttcgcca gatcgcggcg
     5221 cggctgtcgg tcggccgggg cgattcaccg gtgttccaaa gcgatgacgg caccttcatc
     5281 accttgttcg caaggtccga cagcgtcgag gatcatgcct cggcgctgca caatctgttc
     5341 cgcgagccgg tgacgttcgg cccgacccgg ttcgacctca tcgtcacttt gggcgtcgac
     5401 ggcgaggtgg agcgcccgct gaccaggcgg ctcgccgacg cgctcaacgc cgccgacgag
     5461 gctttcggca agggcctcaa gtggaagatg gcggtcgccg agggcgtgga agacaccgaa
     5521 tggcacctgt cgctgctcgg ccagctcgac gaggcgatcg agcgcaagca tatgtgggtc
     5581 gcctaccagc cgaagctcga tcttgcgtcg ggccggctct gcggcgccga agcgctggtt
     5641 cgctgggcgc accccaagcg cggcaatatc agcccgatcg agttcatcca ggcggccgag
     5701 cagagcggcc gcatcgaccg gctgactgcg ttcgtcatgg acgatgcgat cggcgccgcc
     5761 gccgcgctcg ccgcccgcgg caccttcatc gacatgtcgg tcaacctctc cgcccggatg
     5821 ctcggcgacg acggcctggt cgcgatggtg agcggcctcc tccacacaca tcgcttcccg
     5881 ccggagcggc tgatcctcga gatcaccgag accgccgcca tcgacgcgtc ccggcttgcc
     5941 cagctcaccc ggcttcgcga gctcggcgtc ggcctgtcga tcgacgatta cggcaccggc
     6001 ctgtcgacgc tcgaatatct gaagtgcatc cccgcgaccg agatcaagat cgacggctcg
     6061 ttcgtccaga acctctccac ctcgatgagc gaccggttga tggtgagctc caccatcaac
     6121 ctcgcccatg cgctcggccg gcgtgtcgtc gccgaaggcg tcgaggatcc ggccagcctc
     6181 gaggcgcttt cgaccatgca gtgcgacgag gcgcagggct attatatcgg gcggccgatg
     6241 ccgctccggc agctctatcg ggcgctcgcc cggcacgagc ggcgccaggc cgcctgaccg
     6301 cgccgccccg acgcgctcgc cgtccgcgat ccggcgaggc cgctcccacg accctcatcc
     6361 ttccctccgc cgccgcggcg atcgcccgtg cggttcggcg ggacttgcgc gtccggaact
     6421 gtccggcctg cccggcgcga acgtcccgcg ccagccgcgg ccggcggctc gctccagggg
     6481 cgggaagacc gaattgtacc attttgaatg attaccgcga atctaccgta ttaggttaat
     6541 aacttgctaa ccatccgttt cgggtatatc ctcctttcgt aagcgcaacg attatgggcg
     6601 cgcgaaaaaa cagggaggtc ccttatgtgg gcaatcatcc gcgcattctt ctatgcgacc
     6661 accaagatgg gcagcggcgg catcaaaggc tgattccgcg cgtccgaccg ggctgctctc
     6721 gggtcgcgcc gccagccgca acggcctaaa gccgctgccg tctcgggcgc gctcctaagg
     6781 gcgaccgggt attaatatcg gtccagacca ggagcctggc gatgctcgca tcgccaggct
     6841 cagccggttt gggcctggtt tggggcttgg ggctgctcag gctttggctg tcagggtagg
     6901 ggccgttgag gctcaagccg ttgcgactca agccgatttg gctcaagccc gttggggatc
     6961 gagctgctca ggctccggct gttaaggctt gagctgttca ggcttggtct gtcgaggctc
     7021 aacctgctgg cgcccgggcg ctatgccttt ctcgacttca gtcggacgct cggggtgata
     7081 gccgtgcggc tgcagggcat ccgaagagga gcgccagtgc ggcccttcag gaccgtgagt
     7141 gcggccctgc cacgagccct ccagtttagc cttccacgaa tcggcccttg agcggggccc
     7201 gccgaatcgg ccgccagcgc cctcccgaat cggctaacgg tgcgcccccc gaatcggggt
     7261 gccggtgcgt cctgccgcga atcggccacc tttgctcgcc gccgcaaacc cgcccgccag
     7321 tgcccctggg gctgaaccgt catgccagcg aaagctggca tctcacttct ctttgggagc
     7381 cgggcaaaga agaagggaga ttccagcttt cgctggaatg acgaggcggg cttggtggca
     7441 ggggcgcgca tccgcctcgg ccggagcggg cgccgcgaat cggtgtcggc ttggctgcat
     7501 cgaccgggtc cgaatttccc gggaataatc ttgtcgcgcc tccgttgtgg aactgcctga
     7561 acggcatttc aactggaggg atttacatga tcaacaagac gattctggcg accgcctgcg
     7621 ccgccatcgc gctcgccgtc accgccacgc cggccgcggc caaatgggtg aacggggtca
     7681 aggtcgagga caatctgccg cgccacagcg gcgacgacga catggtgaac ggcgtcaagg
     7741 tgcgcggcga cggctcggtc gacgataacg gcccgcggcg caatcgcggc ggccgcggcg
     7801 gcgaggacag cgtcgacagc gacgacgacg acgacaatga cggacgcggc cgcaaccgac
     7861 gcggccgcgg cagcgacgac ggtgtcgata gcgtcgatgg cgacgatgac ggcggccggg
     7921 gccgtgggcg cggccgaggc cggggcagcg acgactcggt cgacagcgtc gatggcgacg
     7981 acgacggcgg aaggggccgt gggcgcggcc gaggacgcgg cggcgacagc gtcgacagcg
     8041 tggacg
//
LOCUS       DASVRK010000171         5485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_468265, whole genome shotgun sequence.
ACCESSION   DASVRK010000171 DASVRK010000000
VERSION     DASVRK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468265"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VF589_06645"
     CDS             <1..1116
                     /locus_tag="VF589_06645"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8257294.1"
                     /translation="FTSYDETVYRLDMARSDAAARETSLAVMREIAGKLAFGRAAVAG
                     AKTAVREEIRARDAAQDGILVAQNRLFAPGAAIARGPVAGTQAEVARATGPALRRLYE
                     TYHVPERTILVLVGDVDPAAAEAEIAALFADWRPRGGPTLDRPAPDRGPPAIPPRRPI
                     ETALYVHRAAPTLVTIAVSAPLGGGADAGRSRDAHFLEHLAGQMLNRRLARLAATPGA
                     PFAAADHAAYDPFATVRLARIELEARDRDWRGALRAGAVELGRALADGFTQAELDAQL
                     AAARAGLARSAAPRTAAALADAIVDAAGRRIVFTEPADPAGTDAYLARIRLPDVNAAF
                     RAVWAGRGRLVFVAHHRRIPGGEAAIAAAWREAAGAR"
     gene            1206..3701
                     /locus_tag="VF589_06650"
     CDS             1206..3701
                     /locus_tag="VF589_06650"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX8257295.1"
                     /translation="MAESTYTDTNNAPVGPATVIVEIPQGSGPAQIPLDVYDPDGDRL
                     WYQMEPRPWPLFGGLEWDPDAGALCYEPFPDFIGVESFTLIVSDGRGGSIEQRVTVAV
                     GQPAPTGENHVPVAPAAIVIVDGRGDIVRQLVTVNVAPVNDTPVPGVAELNTIETAPG
                     SSLSVTLPAGLFTDAEGDAITLSLARGAPSWLSFDPIAGTLSGTVPGGVSGDFAYEIV
                     ATDPAGAVGSAPGTVWVAPADTGPAYTVGGELIVNTHREGWQTYPAVTALEGGNYVVV
                     WETPFNDLDEVFGRIYAADGTRIGAEFRFNSYTAGSQESPSLLALPGGGFVVSWTSVG
                     QDGSGGGVYGQLYGADGARAGAEFRINATTAGYQQLSSMAATGEDSFVVTWLSRPGSR
                     DIAVARRFSAVDGTPLGGEIVLDPATTGSVGQPDIAARADGSWLAVWTRSDGAYQETS
                     HVYAQRFYANGTRAGTIFQVDRAAADGVFSPSVTALAGGGYVVAWTVRGAADDPSETG
                     IHARLFSAGGAALGPEFRVNAAVADYQIHPDVTALAHGGFAIGWTSRGQDGSGDGVYT
                     RLYSAAGVALTPERLVNATVAGDQGYLALAGHENGGYGAAWMTPGQYGSGYEIAARFF
                     APADIRRAGTAGSDYYDGAGGRDTLLGYRGDDRLRGLYGDDWLAGHDGNDRAEGGRGA
                     DRLNGGAGDDTLLGDAGVDRLWGHSGHDVLAGGLGADTLFGGAGADTFLFASALDSGN
                     LPGSYDTIRDFDALDVIDLAAIDADGDAANGDTAFSSVARFTGAGAELRIFRFNGGDL
                     YKVTGDLDGDRAADFTLYVRTDGTILTAADFTG"
     gene            complement(3708..3872)
                     /locus_tag="VF589_06655"
     CDS             complement(3708..3872)
                     /locus_tag="VF589_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257296.1"
                     /translation="MIFGLAPARLLRLYFAAAVTLGAGAYVGGAVTADICAAYDSRTV
                     VGRSPGLLCL"
     gene            4120..4476
                     /locus_tag="VF589_06660"
     CDS             4120..4476
                     /locus_tag="VF589_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257297.1"
                     /translation="MSSKAYLIAAAAAALLLPASAAASAKPGHGGVPPGHLPPPGECR
                     VWKPGVPPGHQPPPTSCRAARRDAYHRGGNVVYGGREGYRGGRYYDGRYRDDRYKHDR
                     KDKSGCDAKDYRKGKC"
     gene            complement(4508..4717)
                     /locus_tag="VF589_06665"
     CDS             complement(4508..4717)
                     /locus_tag="VF589_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257298.1"
                     /translation="MSRVPIKPYLVSKGEDGQFRLVVRETRYNSQGYPLVTATPVEGA
                     FKSATAARAHAKEHFGAEAGQFASK"
     gene            complement(5158..5364)
                     /locus_tag="VF589_06670"
     CDS             complement(5158..5364)
                     /locus_tag="VF589_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257299.1"
                     /translation="MGSALFLAAIIYVAYDTAQGWRTGRIGHRALKGDRKESPHIYWF
                     LMAVNAGLLIGCLLAVLGIYGEPI"
BASE COUNT          771 a         2112 c         1815 g          787 t
ORIGIN      
        1 ttcaccagct atgacgagac cgtctaccgg ctcgacatgg cccgctccga cgccgccgcg
       61 cgcgaaacct cgctcgcggt gatgcgcgag atcgccggca agctcgcctt cggccgcgcc
      121 gccgtcgccg gcgccaagac cgcggtgcgc gaggagatac gcgcccgcga cgccgcccag
      181 gacggcatcc tcgtcgccca gaaccggctg ttcgcgcccg gcgcggcgat cgcccgcggc
      241 cccgtcgccg gcacgcaggc cgaggtcgcc cgcgctaccg ggcccgcgct tcgccggctc
      301 tacgaaacct atcatgtgcc ggagcgcacc atcctggtgc tggtcggcga cgtcgatccg
      361 gccgcggccg aggcagagat cgccgctttg ttcgccgact ggcggccgcg cggcgggccg
      421 accctggatc gtccggcccc ggatcgcggc ccacccgcaa tcccgccccg ccgcccgatc
      481 gagaccgcgc tctacgtcca ccgcgccgcc ccgaccctgg tcaccatcgc cgtgtcggcg
      541 ccgctggggg gaggggccga cgccggccgc agccgcgacg cccatttcct cgaacatctc
      601 gccggccaga tgctgaaccg ccggctcgcc cgcctcgccg ccacccccgg cgcccccttc
      661 gccgccgccg accacgccgc ctacgaccct ttcgccaccg tgcggctggc gcggatcgag
      721 ctcgaggcgc gggatcgcga ctggcgcggc gcgctgcgcg ccggagcggt cgagctcgga
      781 cgggcgctgg ccgacggctt cacccaggcc gagctcgacg cgcagctcgc cgccgcccgc
      841 gccgggctcg cccgctccgc cgccccgcgc acggccgccg cgctggccga cgcgatcgtc
      901 gacgccgccg gccgccgcat cgtcttcacc gagcccgccg atccggccgg caccgacgcc
      961 tatctcgccc gcatccgcct gccggacgtg aacgccgcct tccgcgctgt ctgggcgggc
     1021 cgcgggcggc tggtcttcgt cgcccaccac cgccgcatcc ccggcggcga ggccgccata
     1081 gccgccgcct ggcgcgaagc cgccggcgcc cgctaatccc tcccgtgcaa gcgctttttt
     1141 aacgccgccg cgcttacctc gctccgccgt cgccagcggg cgagcgggaa cttcgagggg
     1201 gaatcatggc ggaatcgacc tacaccgaca ccaacaacgc gccggtcggg cctgccaccg
     1261 tcatcgtcga aatcccccag ggcagcggcc cggcgcagat cccgctcgac gtctacgacc
     1321 ccgacggcga ccggctctgg taccagatgg agccgaggcc gtggccgtta ttcggcgggc
     1381 tcgagtggga ccccgatgcc ggcgcgctct gctacgagcc gttccccgat ttcatcggcg
     1441 tcgaaagctt tactctgatc gtctccgacg gccgcggcgg cagcatcgag caacgcgtca
     1501 cggtcgcggt cggccaaccg gcgcccaccg gcgaaaacca cgtccccgtc gcgccggccg
     1561 cgatcgtgat cgtcgacggc cgcggcgaca tcgtccggca gctcgtcacc gtcaacgtcg
     1621 cgccggtcaa cgatactccc gtccccggcg tcgccgagct gaacacgatc gagacggcgc
     1681 ccggatcgtc gctctccgtc acccttcccg ccggcctgtt caccgatgcc gagggcgacg
     1741 cgatcaccct ctcgctcgct cgcggggcgc ccagctggct cagcttcgat ccgatcgccg
     1801 gcaccctttc cggcacggtc ccgggcggcg tttcgggcga tttcgcctat gagatcgtcg
     1861 ccaccgaccc cgctggcgcc gtcggctccg cgcccggcac cgtctgggtc gctcccgccg
     1921 acaccggccc ggcttacacc gtgggaggcg agctcatcgt caacacccac cgcgagggct
     1981 ggcagaccta ccccgccgtc accgccctcg agggcggcaa ttatgtcgtt gtctgggaaa
     2041 cgcccttcaa cgacctggac gaagtgttcg gccggatcta cgccgccgac ggcacgcgga
     2101 tcggggccga gttccggttc aacagctaca ccgccggaag ccaggaatcg ccgtcgctgc
     2161 tggcgcttcc cggcggcggt ttcgtcgtga gctggacctc ggtcggccag gacggcagcg
     2221 gcggcggcgt ctatggccag ctttacggtg cggacggcgc ccgcgccggc gccgagttcc
     2281 ggatcaacgc cacaaccgcc ggctaccaac agctgtcgtc gatggcggcg accggcgagg
     2341 atagcttcgt cgtcacctgg ctgtcgcgcc cgggatcgcg cgacatcgcc gtcgcccgcc
     2401 gcttcagcgc cgtcgacggt acgccgctcg gcggggagat cgtgctcgat ccagccacca
     2461 ccggatcggt cggccagccc gatatcgcgg cccgggcgga cggctcctgg cttgccgtct
     2521 ggacgcgctc cgatggcgcc tatcaggaaa cctcccacgt ctatgcgcag cgtttctacg
     2581 ccaacggcac ccgcgccggg accatcttcc aggtcgaccg cgccgccgcc gacggcgttt
     2641 tcagcccgtc cgtgacggcg ctggccggcg gcggctatgt cgtcgcctgg acggtgcgcg
     2701 gcgccgccga cgacccaagc gagaccggca ttcacgcccg cctcttctcc gccggcgggg
     2761 ctgcgctcgg ccccgaattc cgggtcaatg ccgcggtcgc ggattaccag atccaccccg
     2821 acgtcaccgc gctcgcccat ggcgggttcg cgatcggctg gacctcgcgc ggccaggacg
     2881 gcagcggcga cggcgtctac acgcgccttt attccgccgc cggcgtcgct ctcacgcccg
     2941 agcggctcgt caacgccacc gtcgccggcg accagggcta cctcgcgctc gccggccatg
     3001 agaatggcgg ctacggcgcc gcctggatga cgcccggcca atatggcagc ggctacgaaa
     3061 tcgccgcccg cttcttcgcc cccgccgata tccgccgcgc cggcacggcg ggctccgact
     3121 attatgacgg cgccggcggc cgcgacacct tgctcggcta tcgcggcgac gaccggctgc
     3181 gcgggctcta cggcgacgac tggctggccg gccatgacgg gaatgaccgc gccgagggcg
     3241 gccgcggcgc cgatcgcctg aacggcggcg ccggcgacga tactttgctc ggcgatgccg
     3301 gcgtcgaccg gctctggggc catagcggcc acgacgtgct ggcgggaggc ctcggcgccg
     3361 acaccttgtt cggcggcgcc ggcgctgaca cctttctgtt cgcctccgcc ctcgacagcg
     3421 gaaacctgcc cggcagctac gacacgatcc gcgacttcga cgccctcgac gtcatcgatc
     3481 tcgccgcgat cgacgccgac ggcgacgccg ccaacggcga caccgccttc agctcggtcg
     3541 cgcgcttcac cggcgccggg gccgagcttc gcatcttccg cttcaacggc ggcgacctct
     3601 acaaggtgac cggcgacctg gacggcgacc gcgcggccga tttcaccctt tacgtgcgca
     3661 ccgacggcac gatcctcacc gccgccgatt tcaccggctg aacccgatca gaggcacagc
     3721 aagcccggcg atcggccgac gaccgtgcgg ctgtcatagg cggcgcaaat gtcggcggtg
     3781 accgcgccgc cgacataggc cccggcgccg agcgtgacgg ccgcggcgaa atagaggcgc
     3841 agcagccgcg ccggagccag tccgaagatc atgccatagc tttcgtcgag tcgcggggga
     3901 tcgtgccgcc gggtgcttgc agcatttccg ccgccggcgg tagcgctgcc tctgcttgac
     3961 agtgatcgac ccatccgatc acccgggggg ccgcgcttat cctgctctag ccgaagcggg
     4021 gcaggggcgg cgacggaacg ccgcgccgct cccgcgggtt cactccaggt ccggcgtgtc
     4081 gttcgcgccg cggccccaca agacagctgg agtagagcca tgtcttcaaa agcgtatctg
     4141 atcgccgccg ccgccgcggc gttgctgctt cccgcctccg ccgccgcctc cgccaaacct
     4201 gggcatggcg gcgtgccccc ggggcatttg ccgccgccgg gcgagtgccg ggtgtggaag
     4261 ccgggcgtgc cgccgggcca ccagccgccg ccgaccagct gccgcgccgc ccgccgcgac
     4321 gcttatcatc gcggcggcaa cgtcgtttac ggcggccggg aaggctatcg cggcggccgt
     4381 tattatgacg gccgctaccg ggacgatcgc tacaagcacg atcgcaagga caaaagcggc
     4441 tgcgacgcca aggactatcg caagggcaag tgctgagccc ctgcctcgga gcctccttcg
     4501 gcgcggctta cttgctggcg aattgcccgg cttcggcccc gaaatgctcc ttggcatggg
     4561 cgcgggccgc ggtcgcgctc ttgaacgcgc cctcgaccgg ggtcgccgtc accagcggat
     4621 agccctggct gttgtagcgc gtctcgcgga cgacgaggcg gaactggccg tcctcgccct
     4681 tggacaccag atagggcttg atcggaactc tcgacattgg ctgtctccag atgaaaaagg
     4741 cccctcccgc gacacggcgg aaggggccga acgatttagc cgatgatacc ggcgacgagg
     4801 cgcctcagct catggcggga aagggtgggc gagcggcgct cggtgcgccg cgtcggcttt
     4861 tgtcccgcgg cggccgcggg cgtgaatggc agcgaaagct tgggccgtac gggcccgata
     4921 gtattgactt gcattgattt ccctggtgcg cggccacgtg gccgcctatt cgtcttgaaa
     4981 ctagtgaacg ccggcgcctc gataaggtgc ccacggccgc ggccgttccg cgaaagccag
     5041 gtagcgtgaa aaggaaggag cggccgtccg gccgccgtat ccgtcgcagg cgacgttagc
     5101 ccacctgata tgggcccaaa ccccccgaca atcaaccgcc cgccgccgcg gccgctttca
     5161 gatcggctcg ccatagatgc cgaggacagc gagcaggcag ccgatcaaca gccccgcatt
     5221 caccgccatc aagaaccaat agatgtgcgg ggactccttc ctgtcgccct tcagggctcg
     5281 atggccgatg cgccccgtcc gccatccctg ggccgtgtcg taggcgacat agatgatcgc
     5341 ggcgaggaag agggcgctcc ccatgctcgg cgtcacgcgc ccggccttct cctgaacggc
     5401 cggcgcggcg ctggagccgg agcggtcgca tcaggcatgg ggccgttcct tgggcacgac
     5461 cttgtatccc gcggcgtcga gcgcg
//
LOCUS       DASVRK010000172         8446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_218866, whole genome shotgun sequence.
ACCESSION   DASVRK010000172 DASVRK010000000
VERSION     DASVRK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218866"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VF589_06675"
     CDS             <1..1052
                     /locus_tag="VF589_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8257300.1"
                     /translation="VEALTQELADQAWALIQEVEAMGGMTMAVAEGMPKRRIEEASAA
                     RAARADRGEDVIVGVNRYRRDDEPPIDILEIDNARVRSGQIDRLDRTRAERDEARAQA
                     ALDGLRDAARENTAQDGAGGGPPPPLAFGERSPSPFRGGVEANLLALSIEAARARCTL
                     GEISAALEDVFGRYDTVPKPVSGVYGGAYAGDPRWQRAGEGVEAVGRRLGRRPRILVA
                     KMGQDGHDRGANLVSSAFADLGFEVVPGPLFQTPEESARMAVEKAVDVVGASSLAAGH
                     KTLVPELIGHLRDLGRPDIKVVVGGVIPAQDYDMLREAGVQAIFGPGTNLVDAAGEVL
                     KLLGHNLPPIEEAAE"
     gene            1069..1443
                     /locus_tag="VF589_06680"
     CDS             1069..1443
                     /locus_tag="VF589_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX8257301.1"
                     /translation="MSSILLALLLASAPDDCANPMAQQEMNRCAARGFEQADAELNAQ
                     WRLTLAAVRAGDGDPIEGDTRPSGEARLREAQRAWVSFRDAHCAVHGYQARGGSLEPL
                     LLDGCRAALTRERTRQLKELTE"
     gene            1440..1805
                     /locus_tag="VF589_06685"
     CDS             1440..1805
                     /locus_tag="VF589_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257302.1"
                     /translation="MIEGEPHQKLRPRPEADNYGETLLYDYSKFLTSMSLLALGGVLS
                     LSQTGDMAGVEPNRLLVILAAVAFGGATGVQVSSGIVSARSGGREPSRWLRRWLQLGT
                     AALALGTGGFLSIFWDSLQ"
     gene            1802..2794
                     /gene="bioB"
                     /locus_tag="VF589_06690"
     CDS             1802..2794
                     /gene="bioB"
                     /locus_tag="VF589_06690"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX8257303.1"
                     /translation="MTTRTDWTRAEIAALFDLPFLDLLFEAQSLHRVHHAPNQVQLST
                     LLSIKTGGCPEDCGYCSQSAFAESGLKADKLMDPEAVLAAAAEAKAAGSGRFCMGAAW
                     REPKERDMDALCTMVAGVKALGLETCMTLGMLTRGQAERLAAAGLDYYNHNIDTSPEH
                     YGAVITTRNFQDRLDTLENVRASGMAVCCGGIVGMGETRGDRVGFLHALASLPEHPES
                     VPINALVPIRGTVLGDMLADTPLARIDDIEFVRTVAVARITMPRAMVRLSAGRESMSE
                     STQALCFLAGANSIFTGDKLLTTGNAGASADAALFAKLGLAPMTAEEPARVAAE"
     gene            2791..3015
                     /locus_tag="VF589_06695"
     CDS             2791..3015
                     /locus_tag="VF589_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257304.1"
                     /translation="MTRLSIQIIFVMLGIAWMLFVTFDLFTSIYGDCAGDRWCEGRKS
                     IAGGLVFWRGFCVTLLIFLAYRFFRKEPDV"
     gene            3008..5161
                     /locus_tag="VF589_06700"
     CDS             3008..5161
                     /locus_tag="VF589_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX8257305.1"
                     /translation="MFKKILIANRGEIACRVIRTAKRMGIASVAVYSDADARAPHVRM
                     ADESVRLGPPPASESYLNAELIIEACKATGAEAVHPGYGFLSERESFARALADNHIAF
                     IGPPPHAIAAMGDKIESKKIARDAGVNVVPGYLGDIATTDEAVTIAAEIGYPVMMKAS
                     AGGGGKGMRLAWSEQDVREGFEATKREGLASFGDDRVFIEKFIETPRHIEIQLLGDQH
                     GNIVYLGERECSIQRRHQKVVEEAPSPFVTPEMRKAMGEQAVALARAVGYYSAGTVEL
                     IVSGADPTGRSFYFLEMNTRLQVEHPVTEEVTGLDLVELMIRVAAGEKLLFVQDDVKL
                     NGWAIENRVYAEDPYRGFLPSTGRLVRYQPPAEKPSVRPEPFDAASGIALGKLRRGTP
                     PSGAEAILLGSARQGVSTSLDTNGRDQPDHLVVRVDDGVAEGGEVSIFYDPMIAKLIT
                     WAPTREQAIDLQIAALDTFVIDGIGHNVDFLSALMQHPRFREGRITTGFIAEEYPDGF
                     HGAPARERLTARLAALAGMLSTAGAERALRIEGQLGTPLEAPTDWMVRIGPLDYPVSV
                     AADRVSAGEGGMTIEMSFVPGQRLIQARIDGDTLSVGVERTILGWRLTTRGARYDARV
                     LTRREAELAGHMIAKVPPDLSRFLLCPMPGLLTALNVGEGDRVEAGQPLAVVEAMKME
                     NILRAEKAGVVKKVEAKPGDSLGVDAVILEFERAS"
     gene            complement(5209..5844)
                     /locus_tag="VF589_06705"
     CDS             complement(5209..5844)
                     /locus_tag="VF589_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX8257306.1"
                     /translation="MTVSLIGRTLLFTAAIALGACGGTGGEGNETGSANTASAAPAAT
                     GEGGDLAAAAPATGGQMAPDKTIGANLAGSPDHSTFAGAVKAAGLEETLTGPTVYTIF
                     APSNAAFQKLPGGTTEGLLKPEAKGQLTGVLTYHIVPGVVAAADLARAVETQGGTAEL
                     ATMAGSSLKVTKSGDALVITDAKGGQARIVQPDMVQSNGVLHVVDTVLMPG"
     gene            complement(5968..7371)
                     /locus_tag="VF589_06710"
     CDS             complement(5968..7371)
                     /locus_tag="VF589_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257307.1"
                     /translation="MRKFLLLASVAAMTVSLPADASSQNRDNRQADRSQNKAEARKAP
                     PRQAANANANGRARSAAKGDNRAPAAVRAKAAANTRADARADAKADDRRAQERRQQAA
                     ANDRKAQDKRQQAAANDRKVQDKRQQAAANDRKAQEQRQQAARQDQRQQAAKRDNVQD
                     RRAAEQRQQALARENQRDRMVREQREDRLARENYQDRMTREQREDVLARQRFQNRLQD
                     QRLDAGVRQRLVVSRDQRYAAAVRAQQVNRWQAAQQRFRADQVQRLRLNTLRQTTPLR
                     YAYRQNPRYRQLSGAYGIGRRLPLSTYRSYNVPIGYRSLYRDTPNSFYRYGDGYVYRV
                     NPYTQLVTGAVPMFGGAFGVGRVMPAGYDVYNVPLQYRSHYYDTPNSFYRYGDGGIYR
                     ADPTSGLITGVVALLAGDLTVGSRLPVGYGAYNLPLQYRDQYVDGPDHMYRYNDGYIY
                     QVDAETMLISSAISALV"
     gene            7996..>8446
                     /locus_tag="VF589_06715"
     CDS             7996..>8446
                     /locus_tag="VF589_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8257308.1"
                     /translation="MSSSNSDARWLDAIVLTVALWLFTLLMFLPVIVERHPGAGFVSI
                     ALDSSTIGVSMLLAMPIFLVFRKTLDWPSGARALILVAAVIFTAVFNRIFDILWTGWV
                     TQNLEASWAAMPQTIERSYDATLKYILVFSVNLALFQLSFSRRRERRQ"
BASE COUNT         1296 a         2779 c         2954 g         1417 t
ORIGIN      
        1 atgtcgaggc gctgacgcag gagctcgccg accaggcctg ggcgctgatc caggaagtgg
       61 aggcgatggg cgggatgacc atggccgtcg ccgagggcat gcccaagcgg cgcatcgagg
      121 aagcctcggc ggcgcgcgcc gcccgcgccg accgcggcga ggacgtgatc gtgggcgtca
      181 accgctaccg ccgcgacgac gagccgccga tcgatatatt ggagatcgac aatgcgaggg
      241 tgcgctcggg gcagatcgac cgcctcgacc ggacccgcgc ggagcgggac gaggcgcggg
      301 cgcaggcggc gctggatggg ctccgcgacg cagcgcgcga aaacaccgcg caggatggtg
      361 cgggtggagg gccccctcca ccgctcgcct tcggcgagcg gtccccctcc ccgttccggg
      421 gaggagttga ggccaacctc cttgcgcttt cgattgaggc cgcgcgggcg cgctgcaccc
      481 tgggcgagat cagcgccgcg ctcgaggacg tgttcgggcg ctacgatacg gtgcccaagc
      541 cggtcagcgg cgtctatggc ggcgcttatg ccggcgatcc gcgctggcaa agggccgggg
      601 aaggcgtgga ggcggtcggg cgccggctgg gccgacgccc gcgcatcctc gtcgccaaaa
      661 tgggccagga cggccacgac cgcggcgcca atctcgtttc ctcggccttc gccgatctgg
      721 gtttcgaggt cgtgcccggc ccgctgttcc agacgcccga ggaaagcgcg cgcatggcgg
      781 tcgaaaaggc ggtcgacgtg gtcggcgcct ccagcctggc cgccggccac aagacgctcg
      841 tccccgagct gatcggccac cttcgcgacc tcggccgtcc cgacatcaag gtggtggtcg
      901 gcggcgtcat ccccgcgcag gattacgaca tgctgcgcga ggccggcgtg caggcgatct
      961 tcggcccggg taccaatctg gtcgatgccg ccggggaagt gctgaagctg ctcggccaca
     1021 atctgccgcc gatcgaggaa gctgccgaat gaagcggtcc ctgcggcaat gagcagtatc
     1081 ctgctcgcgc tcctgctcgc ctccgcgccg gacgattgcg ccaacccgat ggcgcagcag
     1141 gagatgaacc gctgcgcggc gcgcggtttc gagcaggcgg atgcggagct gaacgcgcaa
     1201 tggcgactga cgctagcggc ggtgcgggcg ggcgacggag atccgatcga aggcgacacc
     1261 cgccccagcg gcgaagcgcg gctgcgggag gcgcagcgcg cctgggtgag ctttcgcgac
     1321 gcccattgcg cggtccacgg ctatcaggcg cgcggcggga gcctggagcc gttgctgttg
     1381 gacggctgcc gcgccgcact gacccgggag cggacccggc agcttaagga gctcaccgaa
     1441 tgatcgaggg cgagccgcac cagaagctga ggccccggcc cgaggccgac aattatggcg
     1501 agaccttgct gtacgattat tcgaagttcc tgacctcgat gtcgctgctg gcgctgggcg
     1561 gcgtcctttc cctgagccag accggtgaca tggccggcgt cgagcccaac cggctgctgg
     1621 tgatcctggc ggcggtcgcc ttcggcgggg cgaccggcgt ccaggtgtcg agcgggatcg
     1681 tcagcgcgcg cagcggcggg cgcgagccta gccgctggct gcgccgctgg ctgcagctcg
     1741 gcacggccgc gctcgccctc ggcaccggcg gcttcctcag catcttctgg gattccctcc
     1801 aatgacaacc cggaccgact ggacccgcgc cgagatcgcc gcgctgttcg acctcccctt
     1861 cctcgacctg ctgttcgagg cgcagagcct tcaccgtgtt caccatgcgc ccaaccaggt
     1921 gcagctttcg actttgctgt cgatcaagac cggcggctgc cccgaggatt gcggctattg
     1981 cagccagtcc gcctttgcgg agagcgggct caaggccgac aaattgatgg atccggaggc
     2041 cgtgctcgcc gccgcggcgg aggccaaagc ggccggctcg ggccgcttct gcatgggcgc
     2101 cgcctggcgc gagcccaagg agcgcgacat ggacgcgctc tgcaccatgg tggcgggcgt
     2161 caaggcgctg gggctcgaga cctgcatgac tttgggcatg ctgacccgcg gccaggcgga
     2221 acggctggcg gctgcgggcc tcgattatta caaccacaat atcgacacct cgcccgaaca
     2281 ttatggcgcg gtcatcacca cccgcaattt ccaggaccgg ctcgacacgc tcgagaacgt
     2341 tcgggccagc gggatggccg tgtgctgcgg cggcatcgtc ggcatggggg agacgcgggg
     2401 cgaccgggtc ggcttcctcc acgcgctggc gagcctgccg gagcatccgg aaagcgtgcc
     2461 gatcaatgcg ctcgtgccga tccgcggcac cgtgctcggc gatatgctgg cggacacgcc
     2521 gctggcccgg atcgacgaca tcgaattcgt ccgcaccgtg gccgtcgcca ggatcaccat
     2581 gccgcgcgcg atggtccgcc tctcggccgg ccgcgagagc atgtccgaga gcacccaggc
     2641 gctctgcttc ctcgccgggg cgaacagcat cttcaccggc gacaagctgc tgacgacggg
     2701 caatgcgggt gccagcgccg acgcggcgct gttcgcgaag ctggggctgg cgccgatgac
     2761 cgcagaggaa ccggcgcggg tggcggccga gtgacccggc tttcgatcca gatcatcttc
     2821 gtcatgctcg gcattgcttg gatgctgttc gtgaccttcg acctcttcac gagcatctat
     2881 ggagattgtg ccggcgatcg ctggtgcgaa ggtcgcaaaa gtatcgctgg cggtcttgtt
     2941 ttctggcgcg ggttctgcgt gacgcttctg attttcctcg cctatcgctt cttccgtaag
     3001 gagcccgatg tttaagaaaa tcctgatcgc caatcgcggc gagatcgcct gccgcgtcat
     3061 tcgcaccgcc aagcggatgg gcatcgccag cgtcgccgtc tattcggacg ccgacgcccg
     3121 cgcgccgcat gtgcggatgg ccgacgagag cgtgcggctg gggccgccgc cggcctcgga
     3181 aagttatctc aacgccgagc tcatcatcga agcctgcaag gcgaccgggg cggaagcggt
     3241 gcatccgggc tacggctttt tgtcggagcg cgagagcttc gcccgggcgc tggccgacaa
     3301 ccacatcgcc ttcatcgggc cgcctcccca cgcgatcgcc gcgatgggcg acaagatcga
     3361 atccaagaag atcgcgcgcg acgccggcgt caacgtcgtg ccgggctatc tcggcgacat
     3421 cgccaccacc gacgaagccg tcaccatcgc ggcggagatc ggctatccgg tcatgatgaa
     3481 ggcctcggcc ggcggcggcg gcaagggcat gcggctcgcc tggagcgagc aggacgttcg
     3541 cgaaggtttc gaggcgacaa agcgcgaggg gctggcgagc ttcggcgacg accgcgtgtt
     3601 catcgagaaa ttcatcgaaa ccccgcgcca catcgagatc cagctgctcg gcgaccagca
     3661 cggcaacatc gtctacctcg gcgagcgcga atgctcgatc cagcgccgcc accagaaggt
     3721 ggtggaggaa gcgccgtcgc ccttcgtcac tcccgagatg cgcaaagcga tgggcgagca
     3781 ggccgtcgcc ctcgcccgcg cggtcggcta ttacagcgcc ggcacggtcg agctgatcgt
     3841 ctcgggcgcg gacccgaccg gccggagctt ctacttcctc gagatgaaca cccggctgca
     3901 ggtcgagcat ccggtgaccg aggaagtgac cgggctcgat ctggtcgagc tgatgatccg
     3961 ggtcgcggcc ggggagaagc tgctcttcgt tcaggatgat gtgaagctca acggctgggc
     4021 gatcgagaac cgcgtctatg ccgaggaccc gtatcggggc ttcctgccgt ccacggggcg
     4081 gctggtgcgg taccagccgc ctgctgaaaa accctccgtt cgtcccgagc ccttcgacgc
     4141 cgcctccggc atcgctctgg gtaaactccg tcgagggacc ccgccgagcg gagccgaggc
     4201 catccttctc ggctccgctc gacagggtgt ctcgacttcg ctcgacacga acggcaggga
     4261 tcagcccgac cacctcgttg tccgagtcga tgacggcgtc gcggagggcg gcgaagtctc
     4321 gatcttctac gatccgatga tcgccaagct catcacctgg gcccccaccc gcgagcaggc
     4381 gatcgacctc cagatcgcgg cgctcgatac gttcgtgatc gacggcatcg gccacaatgt
     4441 cgatttcctg tccgcgctga tgcagcaccc gcgctttcgc gagggccgca tcaccaccgg
     4501 cttcatcgcc gaggaatatc ccgacggctt tcacggcgcg ccggcgcgcg agcggctgac
     4561 ggcgcggctg gcggcgctgg cgggaatgct ctcgaccgcc ggggcggagc gcgcgctgcg
     4621 gatcgagggg cagctcggca cgccgctgga ggcgccgacc gactggatgg tgcggatcgg
     4681 gccgctcgat tatccggtca gcgtcgccgc cgaccgcgtc agcgcgggcg agggcgggat
     4741 gacgatcgag atgagcttcg tgcccgggca gcgcctgatc caggcgcgga tcgacggcga
     4801 cacgttgtcg gtcggggtgg agcggacgat cctcggctgg cggctcacca cgcgcggcgc
     4861 ccgctatgat gcgagggtgc tgacccggcg cgaggccgag cttgccgggc acatgatcgc
     4921 caaggtgccg cccgatcttt cgcgcttcct gctgtgcccg atgccggggc tgctgaccgc
     4981 gctcaacgtc ggcgagggcg accgcgtcga ggccggccag ccgcttgcgg tggtcgaggc
     5041 gatgaagatg gagaacatcc tgcgggcgga aaaagccggc gtggtgaaga aggtcgaggc
     5101 gaagcccggc gacagcctgg gcgtcgacgc cgtcatcctg gagttcgagc gcgcgagcta
     5161 ggccttctgc cggctccggc agaagtgcgc ccagctttcg ccccgggcct agcccggcat
     5221 cagcaccgta tcgacgacgt gcagcacgcc gttcgactgg accatgtccg gctggacgat
     5281 gcgggcctgg ccgcccttgg cgtcggtgat caccagcgcg tccccggact tggtgacctt
     5341 gaggctgctg ccggccatgg tggcgagctc ggcggtgccg ccctgggtct cgacggcccg
     5401 ggcgaggtcc gccgccgcca ccacgccggg aacgatgtga taggtgagga caccggtcag
     5461 ctggcccttc gcttcgggct tgagcaggcc ttccgtcgtg ccgcccggca gcttctggaa
     5521 ggcggcgttg ctgggcgcga agatcgtata gacggtcggt ccggtcagcg tttcctcgag
     5581 cccggccgcc ttgaccgcgc cggcgaaggt cgaatggtcg ggcgagccgg cgagattggc
     5641 gccgatcgtc ttgtcgggcg ccatctgccc gcccgtcgcg ggggccgcgg cggcgaggtc
     5701 gccgccctcg cccgtggccg caggcgccgc actggccgta ttggccgaac cagtctcatt
     5761 gccctcgccg ccagtgccgc cgcaggcgcc gagcgcaatg gccgcggtga acaacaaggt
     5821 cctgccgatc agggagactg tcatctttgt actcctttaa gaaactcaag catctttacg
     5881 cgcgagcgcc gtcacggttg cgttacgagc ggcccaaagg aaaaggggcc gcgcctggcg
     5941 cagccccttc ccttttcgtc cgccgcctca gaccaaagcg gaaatggcgc tcgaaatcag
     6001 catcgtctcg gcgtcgacct ggtagatgta gccgtcattg taacgataca tatggtccgg
     6061 gccgtcgaca tattggtcgc gatactggag cggcagattg taggcgccgt agccgaccgg
     6121 gagcctgctt ccgaccgtga gatcgccggc cagaagcgcc accaccccgg tgatcaggcc
     6181 gctggtcgga tcggcccggt agatgccgcc atcgccatag cgatagaagc tgttgggcgt
     6241 gtcgtaataa tgcgagcgat actggagcgg cacgttgtag acgtcgtagc cggccggcat
     6301 cacgcgcccg accccgaagg cgccgccgaa catcgggacc gcgccggtca ccagctgggt
     6361 gtaggggttc acccgataca catagccgtc gccatagcgg tagaagctgt tgggcgtatc
     6421 acgatagagg ctgcgatagc cgatcggcac gttgtagctg cggtaggtcg acaagggcag
     6481 ccggcggccg atgccatagg ctcccgacag ctgccggtag cgcgggttct ggcgataggc
     6541 gtaacggagc ggcgtcgtct ggcggagcgt gttcagccgc aggcgctgga cctggtcggc
     6601 gcggaaacgc tgctgggcgg cctgccagcg attgacctgc tgggcgcgga cggcggcggc
     6661 gtagcgctgg tcgcgtgaga ccacgagacg ctggcggaca ccggcatcga gcctttgatc
     6721 ctgcaaccgg ttctggaagc gctggcgggc cagcacgtcc tcacgctgct cgcgcgtcat
     6781 ccgatcctgg taattttcgc gggcgagccg atcctcgcgc tgctcgcgga ccatgcggtc
     6841 gcgctggttc tcgcgggcga gggcttgctg cctttgctcg gcggcgcgcc gatcctgaac
     6901 gttgtcgcgc ttggccgcct gctggcgctg gtcctggcga gccgcctgct gacgctgctc
     6961 ctgagccttg cgatcgttgg cggcggcttg ctggcgcttg tcctgaacct tgcggtcgtt
     7021 ggcggcggcc tgctggcgct tgtcctgagc cttgcggtcg ttggcggcgg cttgctggcg
     7081 gcgctcctgg gcgcggcggt cgtcggcctt ggcgtcggcc cgcgcgtcgg cacgggtgtt
     7141 ggcggcggct ttcgcgcgga cggcagcggg cgcacgattg tcgcccttgg cggcggagcg
     7201 ggcgcggccg ttggcgttgg cattggcagc ctggcgcgga ggagccttgc gggcctcggc
     7261 cttgttctgg ctgcggtcgg cctggcgatt gtcgcgattc tggctgcttg cgtcggccgg
     7321 aagggaaacc gtcatggcgg cgacgctggc tagcaataga aacttacgca taaccctaac
     7381 tcctcttgtt gcggtaagaa cggatggggg atgagccgcg ttccggctca aatccttcgc
     7441 ctcaccgcac taagtcgaca tatgggcccg gtaagttgaa cggggtttga atagttcgtt
     7501 catgaagccg ttcatggagt gaagccgccc cgaggccttg cagattcatc gtgccggaag
     7561 ccgcttacgt cgcagcccgc aggcttcgca gcagcgttcg tcgattcgaa cgcgggagcc
     7621 gcgacggcgt gcaaaagcgg ggttattcgg caggttctgg agggcaccag gcgcggatcg
     7681 aggatgatct tgctcgcgca ccacgtacga ggatctgaaa atgactgccc ctgcccttcc
     7741 gcaacccgcc cctggccggc gccgctgatg ctcgtccagc ttcgggaaac gatggccgcc
     7801 gggcggccaa atccctcgcg cgacacattt ttgtcgcgcg aacgcgcact ttcgtctcgc
     7861 cactataggc ttaagccggt tctcgcgcga ccctggacgg atgcgacgac cgcttaccgg
     7921 atgcttatga cgagtgacag cctcgcggct ttgcggcaaa ttgggccgct ggtcaggcga
     7981 ggtcccatca cgttcatgtc gtcatccaat agcgatgccc gttggctcga cgcgatcgtc
     8041 ctcaccgtcg ctttgtggct gttcacgttg ctgatgttcc tgccggtgat cgtcgagcgg
     8101 cacccgggcg ccggcttcgt cagcatcgcg ctcgacagct cgaccatcgg cgtgtcgatg
     8161 ctgctggcga tgccgatctt cctcgtattc cgcaaaacgc tcgactggcc gtcgggcgcg
     8221 cgcgcgctga tcctggtggc ggcggtgatc ttcacggccg tgttcaaccg catcttcgac
     8281 atattgtgga ccggctgggt cacccagaat ctggaagcga gctgggcggc gatgccgcag
     8341 acgatcgagc gcagctacga tgcgacgctc aaatatattc tcgtcttctc ggtcaacctg
     8401 gccctgttcc agctgagctt ctcgcggcgg cgcgagcggc gccagg
//
LOCUS       DASVRK010000173         5333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_148440, whole genome shotgun sequence.
ACCESSION   DASVRK010000173 DASVRK010000000
VERSION     DASVRK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148440"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF589_06720"
     CDS             complement(<1..193)
                     /locus_tag="VF589_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257309.1"
                     /translation="MSTAPDTERDLLAAEYALGVLAGADRAEAERLIGADPAFAAAVA
                     EWQERLSPLIEEVDPVAPDA"
     gene            complement(190..759)
                     /locus_tag="VF589_06725"
     CDS             complement(190..759)
                     /locus_tag="VF589_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8257310.1"
                     /translation="MNDAPRATRQRGRLADDLMKVAGGDRSAFAEVYRSTSAKLFGIC
                     LRILNNRSEAEEALQEAYVNVWRKAEAFDPARSSPITWLAALARNKAIDRLRARGSRP
                     AEALGVEALALPDPAASAPERIEASEERRQLSRCIDELETNQARAIRTAFFRGATYAE
                     LAAEERVPLGTLKSWIRRGLLRLRECLER"
     gene            1246..1794
                     /locus_tag="VF589_06730"
     CDS             1246..1794
                     /locus_tag="VF589_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257311.1"
                     /translation="MGTQTKPADGALTLAEIKEFASFSKGTQRYIRRALDVGLGRRDA
                     MKRWSRDVGEAASIRAQARVYQRLEHIRDYVPDDSGLDVMEPLMGPLVTMTAFDLGQD
                     RLPDFASYRFLYERLIGACVRPWLPGAFCAAAALPHLHPEKRRALLQSISEAAATAPG
                     WSNREPTFFPEWVEKVDVTVAG"
     gene            complement(1884..2825)
                     /locus_tag="VF589_06735"
     CDS             complement(1884..2825)
                     /locus_tag="VF589_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8257312.1"
                     /translation="MGFAEAAEAELRALLGLLRTERYRFVTPTPATHARVIARPGKRE
                     ARGLRDIFGWNLPFAPDLLPGGLLDGLVAAGLAEPTGGLWRSRVRVASLGERLFVHSA
                     FPTVAVDAVFFGPDTYRFAAFLKAELPRVGGARRLVDLGAGAGAGGIVAAALLPGARI
                     TLVDVNPAALGLARVNAADAGINAEFVEAGSLDEVAGRIDLVIANPPYIMDEGDRTYR
                     DGGGMHGAGVSFDWALAAARRLEPGGRMLLYTGVALVEGEDELEAALERALPELGCAL
                     RYRELDPDIFGEELDKPPYTEVERIAAVGAVIEKAGG"
     gene            complement(2837..3073)
                     /locus_tag="VF589_06740"
     CDS             complement(2837..3073)
                     /locus_tag="VF589_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257313.1"
                     /translation="MIAGYSFGGCSQEQPEPPPGELEKFVARIDAKTQAEHAELVKAS
                     RAREEERARQSEERLRQLENAPPAALESAVKSIR"
     gene            3191..3622
                     /locus_tag="VF589_06745"
     CDS             3191..3622
                     /locus_tag="VF589_06745"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8257314.1"
                     /translation="MITRTASARYEGFGKDGRGHVSLGSGVLADQPYGFGTRFGEEPG
                     TNPEELVAAAHAACFTMALSFALAKAGFADGTLATTARVKLDRDGDGFKVSRSDLVLE
                     GTVADIDPARFAALAEEAKANCPISKLLNAEMTLEQRLNPA"
     gene            3644..4912
                     /locus_tag="VF589_06750"
     CDS             3644..4912
                     /locus_tag="VF589_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8257315.1"
                     /translation="MTSKIFEFRGAEGHQLAGRLELPEGPAFASALFAHCFTCSKDSL
                     AAVRVARALAAAGIAVLRFDFTGLGDSDGSFGERGFSGDVRDLVAAAKAMADAGAAPQ
                     LLVGHSLGGAAILAAAAELPDVAAIATIAAPFDVEHITGHFREALGEIAEHGRAEVTL
                     GGRPFTIGKAFVDDLGRHDQAARIAALGRPLLILHSPVDQVAGIENATRIYGAARHPK
                     SFVSLDGADHLLTRQPDAEFAARIIAAWASRYLPRAPALELPGSERRVIAEPTGEGRF
                     QTRIEVRGGAIYADEPVEVGGSDTGPTPYELLCAALAACTAMTMRLYAEGKKWELPPL
                     RVKVAHAALPQQPPRDRFTREILFDGPLDAERRARLLEIADRCPVHRTLERSSEVVTI
                     ESDMAPPEIAAEPASQHVEDMGELSRDAAE"
     gene            4989..>5333
                     /locus_tag="VF589_06755"
     CDS             4989..>5333
                     /locus_tag="VF589_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257316.1"
                     /translation="MSAESINTPGWPMNRKPIFDAVRVLLNRGFTPADIEVLDRACDL
                     AEAAVDPDRRPPAPPPARPAPAPSAVRAHVLGGLSEQFESGGRGPGTVSSGVGDPGGV
                     SYGVYQLSSKKGA"
BASE COUNT          790 a         1977 c         1767 g          799 t
ORIGIN      
        1 ccgcgtcggg cgccaccgga tccacctcct cgatcagcgg cgaaaggcgc tcctgccatt
       61 ccgcgaccgc ggcggcgaag gccgggtccg cgccgatcag ccgttcggcc tcggcccggt
      121 ccgcgccggc gagcacgccg agcgcatatt cggcggcgag caggtcgcgt tcggtatcgg
      181 gcgcggtgct catcgctcga gacactccct cagccggagc aggccgcgcc ggatccagct
      241 cttgagcgtc ccgagcggga cccgttcctc ggccgccagc tcggcatagg tggcgccgcg
      301 gaagaaggcc gtgcggatcg cccgggcctg gttggtttca agctcgtcga tgcagcgcga
      361 cagctgccgc cgttcctccg acgcctcgat ccgctccggc gcggacgcgg ccggatcggg
      421 gagcgccaac gcttcgaccc cgagcgcctc ggccgggcgc gatccgcgcg cgcgcagccg
      481 gtcgatcgcc ttgttgcggg cgagcgccgc cagccacgtg atcgggctgg atcgcgccgg
      541 atcgaaggcc tccgccttcc gccacacatt cacataggct tcctgcaagg cttcctcggc
      601 ttcgctcctg ttgttcaaga tacggaggca gatgccgaaa agcttcgccg acgtgctgcg
      661 atagacctcc gcgaacgccg agcggtcgcc gccggccacc ttcatcaagt cgtcggcgag
      721 ccggccgcgc tggcgggtcg cacgcggggc gtcgttcatg gggagggtct ggcacgcccc
      781 ggcaggctcg gcaagcgcgg cgcccacccc cataccagcg aaagcgcatc gtcatcccag
      841 cgaaagctgg gatctccctt cttctccgcc gggaagggaa gaagtgggat tccagcttgc
      901 gctggaatga cggggagtgc cgtggttccg cttcgtcatc cgaccactgc gaccagtccc
      961 ggccccggcg ctccccaaaa ccgccgcgca agcctttcgt cgcaaaataa aaaagctgtg
     1021 gaaaagccgc ttgcattgtc gcggcaccgc ggattcggct cgggcgctat gcggtcatgt
     1081 tgctttgatg tgacagtctg atgaagctcc gtaagcaaat ggaacaatat cgttgcagcc
     1141 catgtgcttc tctgattcaa tattaacggg aggcggggaa gtgattaagc gtcgcgtaac
     1201 caaattgggc gcatgatccc ccccgagggc aaaaggagtg ggatcatggg tacgcagacc
     1261 aagcccgccg acggcgcgct gaccctggct gaaataaagg aattcgcgag cttctcgaaa
     1321 ggcacgcagc gttacatccg ccgcgcgctc gatgtcgggc tcggccgccg ggatgcgatg
     1381 aagcgctggt cgcgcgacgt cggcgaggcc gccagcatcc gcgcccaggc gcgggtctat
     1441 cagcggctcg aacatattcg cgactatgtg ccggacgata gcggcctcga tgtgatggag
     1501 cccctgatgg gcccgctcgt caccatgacc gccttcgacc tcggccagga ccggcttccc
     1561 gacttcgctt cctaccgctt cctctacgag cggctgatcg gggcctgcgt gcggccgtgg
     1621 ctgcccggcg cgttttgcgc ggcggcggcg ctgccgcatc tccacccgga aaagcgccgc
     1681 gctttgctcc agtcgatcag cgaagccgcc gccaccgcgc cgggctggtc gaaccgggag
     1741 ccgaccttct tccccgaatg ggtcgagaag gtcgacgtca ccgtcgccgg ctgacccgag
     1801 tacgcatcgg caggcgccct cccctcgccg ggggagggcg tcgccggctt gacctctacg
     1861 cctccccgat ctccggttcc gcctcaaccg ccggccttct cgatcaccgc cccgaccgcc
     1921 gcgatccgct cgacctcggt ataaggcggc ttgtccagtt cctccccgaa tatgtccgga
     1981 tcgagctcac gatagcggag cgcgcagccc agttccggca gcgcccgttc cagcgccgcc
     2041 tcgagttcgt cctcgccctc gaccagggcg accccggtat agagcagcat gcgtccgccc
     2101 ggctccagcc gccgcgccgc cgccagcgcc cagtcgaacg acacgcccgc cccgtgcatg
     2161 ccgccgccgt cgcgataggt gcggtcgccc tcatccatga tgtagggcgg gttcgcgatc
     2221 acgaggtcga tgcggccggc gacctcgtcc aggctcccgg cttcgacaaa ttcggcgttt
     2281 attccggcat cggcggcgtt gacccgcgcg agtccgagag ccgcagggtt gacgtcgacg
     2341 agcgtaatcc gggcgccggg cagcagcgcc gccgccacga tcccgcccgc gcctgccccg
     2401 gcgccgaggt cgaccagccg ccgcgcgccg cccacccgcg gcagctcggc cttaaggaaa
     2461 gcggcgaagc gataggtgtc gggtccgaag aaaaccgcat ccaccgccac ggtcggaaaa
     2521 gcggaatgaa cgaatagcct ctcgccgagg ctggcgacgc gcacccggct cctccacaag
     2581 ccgccggtcg gctcggccag cccggccgcc acgagaccgt cgagcagacc gcccggcaac
     2641 aggtccggcg cgaacggcag gttccagccg aaaatgtcgc gcaagcctcg cgcctcgcgc
     2701 ttgccgggcc gggcgatcac ccgcgcgtga gtggccggag tcggagtgac gaagcgatag
     2761 cgctcggtgc gcagcaggcc gagcaaggcg cgcagctccg cttcggcggc ttcggcgaaa
     2821 cccatattca tccccctcag cggatcgact tgaccgcgct ctcgagcgcc gccgggggcg
     2881 cgttctcgag ctggcgcagc cgctcttcgg attggcgcgc ccgctcctcc tcccgcgccc
     2941 gcgacgcctt gacgagctcg gcatgctcgg cctgcgtctt ggcgtcgatc cgcgcgacga
     3001 acttctcgag ctcgccgggc ggcggctccg gctgttcctg gctgcagccg ccgaaactat
     3061 agccggcgat catgattgca gcgattttag ctcggctcca catgcgctca taaacacccg
     3121 aacctgcatt gcgggtgcag cccggcccgc atatgcggtg tccggctgcc ttggcttgag
     3181 gagacgaacc atgatcaccc gcaccgcatc ggcccgctat gagggcttcg gcaaggacgg
     3241 gcgcggccac gtctcgctgg gctcgggcgt gctcgccgac cagccttacg gcttcggcac
     3301 ccgcttcggc gaggagcccg gcaccaaccc cgaggagctg gtcgccgccg cccacgccgc
     3361 ctgcttcacc atggctttgt cgttcgcgct cgccaaagcg ggcttcgccg acggcacgct
     3421 ggcgacgacc gcgcgcgtca agctcgaccg cgacggcgac ggcttcaagg tctcgcgctc
     3481 agacctcgtc ctcgaaggca cggtggcgga catcgacccg gcccggttcg ccgctctggc
     3541 cgaggaggcc aaggccaatt gcccgatctc caaattgctc aatgccgaaa tgacgctgga
     3601 gcagcggctc aacccggcct agacgcgggg gcgggagggt ccgatgactt cgaagatatt
     3661 cgagtttcgc ggcgctgagg ggcatcagct cgccggccgg ctcgagcttc cggagggccc
     3721 ggccttcgct tcggccctgt tcgcccattg cttcacctgc tccaaggatt cgctggcggc
     3781 ggtgcgggtc gcgcgcgcgc tggcggcggc cggcatcgcc gtgctccgct tcgatttcac
     3841 cggcctcggc gacagcgacg gcagcttcgg cgagcgcggc ttcagcggcg acgtgcgcga
     3901 cctcgtcgcc gccgcgaagg ccatggccga cgccggcgcc gcgccgcagc tgctggtcgg
     3961 ccacagcctc ggcggcgccg ccatcctcgc cgccgccgcc gagcttccgg acgtcgccgc
     4021 gatcgccacc atcgccgcac ctttcgacgt cgagcatatc accggccatt tccgcgaggc
     4081 gctgggcgag atcgcagagc acggccgcgc cgaagtgacg ctcggcggcc ggcccttcac
     4141 catcggcaag gcgttcgtcg acgatctcgg ccggcacgat caggcggcgc gcatcgccgc
     4201 gctcgggcgg ccgctgctca tcctccactc gcccgtcgac caggtggccg ggatcgagaa
     4261 cgccacccgc atctacggcg ccgcccgcca tcccaagagc ttcgtctcgc tcgacggcgc
     4321 cgaccatctg ctgacgcgcc agcccgacgc cgagttcgcg gcccgcatca tcgccgcctg
     4381 ggcgagccgc tatctgccca gggcgccggc gctcgaactg cccggctcgg agcgccgcgt
     4441 gatcgccgag ccgaccggcg aaggccgctt ccagacccgc atcgaggtcc gcggcggcgc
     4501 catctatgcc gacgagccgg tcgaggtcgg cggctccgac accggcccca cgccctatga
     4561 attgctgtgc gccgcgctcg ccgcctgcac ggcgatgacc atgcgcctct atgccgaggg
     4621 caagaaatgg gagttgccgc cgctccgggt caaggtcgcc catgccgcgc tcccgcagca
     4681 gccgccccgc gaccgcttca cccgcgaaat attgttcgac ggcccgctcg acgccgagcg
     4741 ccgagcccgc ctgctcgaaa tcgccgaccg ctgcccggtc caccgcacgc tcgagcgcag
     4801 ctccgaggtg gtgacgatcg agagcgacat ggctccgccc gagatcgcgg ccgagcccgc
     4861 cagccagcat gttgaggaca tgggcgagct cagccgcgac gccgcggagt aagcgccgcg
     4921 cgggccggcg cccgccgcga tagtcggtgc ggggccctgt ccttgcgttg cggccgcaga
     4981 ggtccactat gtcggccgag tcgatcaaca ccccggggtg gccgatgaac cgcaagccga
     5041 tcttcgatgc cgtgcgcgtc ctgctgaacc gtggcttcac cccggcggac atcgaggtcc
     5101 tcgaccgggc ctgcgacctg gccgaggccg ccgtcgaccc ggaccggcgg ccgcccgcgc
     5161 cgcctcccgc caggccggcg ccggcccctt cggcggtgag ggcgcatgtg ctgggcgggc
     5221 tgtccgagca gttcgagagc ggcgggcgcg ggccgggcac ggtgtcgtcc ggcgtgggcg
     5281 atccgggcgg cgtctcctac ggcgtctatc agctctcttc gaaaaagggt gcc
//
LOCUS       DASVRK010000174         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_219732, whole genome shotgun sequence.
ACCESSION   DASVRK010000174 DASVRK010000000
VERSION     DASVRK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219732"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..712
                     /locus_tag="VF589_06760"
     CDS             206..712
                     /locus_tag="VF589_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEX8257317.1"
                     /translation="MFKLVTVTALLLCAAGASAQSTDFGVRAVGGFAAPGASPVAAEP
                     LRLSAPVSIGAVASIGGRWGRVTSTLRSPQHNRRVGGARNSYHIRGRAIDIARRAGVR
                     HAEIAAAFRAAGYHLVESLDEGDHSHFAFGGPTRARPFQSAATSARAASADVTQWRVV
                     SAPRTHSR"
     gene            complement(741..1220)
                     /locus_tag="VF589_06765"
     CDS             complement(741..1220)
                     /locus_tag="VF589_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257318.1"
                     /translation="MAKSAGGKSGGKKREAKASKPAGKGKPGKNAAPKPDKPAKAPKP
                     VKAAPAAQPAPRKSVNAADVVRQAKEKATALANNPAVAEVVAATLFAAAAALKNPAGA
                     RRLAADVADELGTATKGAGGKGAEFWRLALDVARRSVEAMGEGEGDATKAKGGKTAK"
     gene            1531..>1894
                     /locus_tag="VF589_06770"
     CDS             1531..>1894
                     /locus_tag="VF589_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX8257319.1"
                     /translation="MRAPVPAPAEVIGPRYFNRELSWLAFNRRVLEEACNPKHPLLER
                     LRFLSISGSNLDEFFMVRVAGLKGQQLQDVEQRSADGLTPGQQLAGIVAEVDRLVSSQ
                     QAVWASLSADLARAGIEVL"
BASE COUNT          245 a          684 c          631 g          334 t
ORIGIN      
        1 gctggcgagc agcgccgccc ctgatgattg ccgaggaccc catcgttcaa cttttctgac
       61 ggacatgccg cgtcctagca gattggcctc ggctccgcga ttctttccat caaaagcgcc
      121 agcttggtta ctcaacataa aattaacagg ttcctttggg cgttcggcct gtgctatggc
      181 gatccgcggc cgagggggtc accagatgtt caagctcgtc accgtcactg cattgcttct
      241 ctgcgcggcg ggcgcatcgg cgcaatccac cgattttggc gtgcgagcgg ttggcggctt
      301 cgctgcgcca ggcgcgtccc ccgtggccgc tgagccgctg cggctgtccg cgcctgtcag
      361 catcggcgcc gtcgccagca tcggcggacg gtggggtcgc gtgaccagca cgcttcgctc
      421 gccgcaacat aaccgccggg tcggcggggc gcgcaacagc tatcatatcc gcggccgtgc
      481 gatcgacatc gcccgccgcg ccggcgtgcg ccatgccgag atcgcagcgg cctttcgcgc
      541 ggccggttat catctcgtcg agtcgctcga cgagggcgac cacagccact tcgcgttcgg
      601 cggcccgacg cgggcgcggc cgttccagtc agcagccacc tcggctcggg ccgcgtccgc
      661 cgacgtcacg caatggcgcg tcgtctcggc cccgcgcact cactcgcgct aggatccggc
      721 ggagcttcgg cacccccctt ctatttagcg gtcttcccgc ccttcgcttt cgtcgcgtca
      781 ccctcaccct cacccatcgc ctcgacactc cgccgggcca cgtccagcgc gagtcgccag
      841 aattcggcgc ctttgccgcc ggcgcctttc gtcgcggtgc cgagctcgtc cgccacatcc
      901 gcggccaggc gccttgcccc ggcggggttc ttgagggccg cagcggcggc gaacaaagtc
      961 gccgcaacca cttcggccac tgccgggttg ttggcgagtg cggtggcttt ctccttcgcc
     1021 tggcggacaa cgtcggcggc gttcacgctc ttgcgcggag cgggttgcgc ggctggcgcc
     1081 gccttcacgg gcttgggcgc cttggccggc ttgtcaggct tgggagcggc attcttcccc
     1141 ggcttcccct tgccggccgg tttggaggct ttcgcctcgc gcttcttgcc gccgcttttg
     1201 ccgcctgccg atttcgccac gtgcctgtct cctggggttg attgcgccgc gccgggcggt
     1261 tcgttccagc atagcaacag tcccgcgcac gccacaaccg ggcgcccgtc ctgtttgcac
     1321 gcgggcgccg ggggcatata ggcgggcgat gcaagcagag cggataagca ggtaatggac
     1381 agcctggcga aacaggaagc gcccgccaag tccggcgccg ccgtggagcc ggctgcggag
     1441 gaggtcacgg ccgaagcagc gccgcccaag gcgccctcac cggcgccgcc tcggcggcgc
     1501 gggccgccca caacgactga ggcggactcc atgcgcgcgc cagtgcctgc gcccgccgag
     1561 gtgatcggac cgcgctactt caaccgcgag ctgagctggt tggcgttcaa ccggcgggtg
     1621 ctggaggagg cgtgcaaccc gaagcatccg ctgctcgagc ggctgcggtt cctgtcgatc
     1681 tctggctcca acctcgacga attcttcatg gtccgcgtgg ccgggctgaa gggccagcag
     1741 ttgcaggacg tagagcagcg ctcggccgac gggctcaccc ccggccagca gctcgccggc
     1801 atcgtcgcgg aggtcgatcg cctcgtctcg agccagcagg cggtgtgggc gtcgctctcg
     1861 gccgacctcg cgcgcgccgg gatcgaggtg ctcg
//
LOCUS       DASVRK010000175         5337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_343442, whole genome shotgun sequence.
ACCESSION   DASVRK010000175 DASVRK010000000
VERSION     DASVRK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343442"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF589_06775"
     CDS             <1..343
                     /locus_tag="VF589_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257320.1"
                     /translation="VSVDILHDGVGTLKVAVADLIDRKPERTDQKGAERLPGKVREHL
                     EALDWVEKADVRMREEGHIFVGEAFVIPKAGTRDLIAKIERAVAEAKAIDWRMHDLVV
                     QPVADLPPYKP"
     gene            404..964
                     /locus_tag="VF589_06780"
     CDS             404..964
                     /locus_tag="VF589_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX8257321.1"
                     /translation="MGTVINFEDRAVAKLRARVAAAEEANQDLIAFARGHSGAVASIH
                     EAVLAAVEAEGFDHLIHIVTQEWPQILGLDAVAVALFVGGKGMRADASGLQFVNPRVI
                     ERSISQFEGVVLRGCKRGHPLFGPACDLIRAEALVRLDSDAPLPKGLLALGQRGEQGF
                     ETRHGSELLVFLGRVLSRCMGRWLIP"
     gene            949..1872
                     /locus_tag="VF589_06785"
     CDS             949..1872
                     /locus_tag="VF589_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX8257322.1"
                     /translation="MADPLADHPARQEALRWQDHLSQSRRRSAHTVRAYAATAHRLID
                     FLAGYSGEAVTADALTRLRASDLRAFLTRRRAEGLGNSSAARELSAVRGFLGFVAEQQ
                     GSAAALPRLKGPKRPRSVPRPISPAEAVALAEDVAEDAAEPWIADRDYAVLMLLYGSG
                     LRVAEALGLTGAALPLGEALSVTGKRNKTRMVPLLAPVRHAIEAYVAACPYPAAREEP
                     LFRGARGGPLNGEMVRRAVRKARVRLGLSDRTTPHALRHSFATHLLGRGADLRSLQEL
                     LGHASLSSTQIYTAVDTAHLMDVYRNAHPRA"
     gene            complement(1879..2478)
                     /locus_tag="VF589_06790"
     CDS             complement(1879..2478)
                     /locus_tag="VF589_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX8257323.1"
                     /translation="MGDWVRELIEQSGYVGVCLLMLLETVFPPIPSEVIMSLSGLQAQ
                     RGTMTLWGAILAGTLGAMLGNIMWYWVARALGIERFYPLIDRFGRFLTLDRREVERAD
                     ALFDRYDRWVVFLGRMVPTIRSLVSIPAGLFGMGWRAFLFWSTLGTLGWTTALAVAGY
                     LLGQNYTEVDRYLGPVSTGIVAVLVLWYVFRVVTWKPRR"
     gene            complement(2471..3424)
                     /gene="gshB"
                     /locus_tag="VF589_06795"
     CDS             complement(2471..3424)
                     /gene="gshB"
                     /locus_tag="VF589_06795"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX8257324.1"
                     /translation="MSLRVAVQMDPMEGINIAGDSTFAIMLSAEARGHRLFHYEAGDL
                     SYRAGRVSALARPVSVQRVEGDYFRFGEPVRLDLGEDADVVLMRQDPPFDLAYITATH
                     LLERIHPRTLVVNDPASVRNAPEKLFVLDYAEFMPPTLITRSLDETRAFHAEHGEVVV
                     KPLYGNAGAAVFHVGRSDANLAALTELFGEVWREPFMVQAFLPDVSLGDKRIVLVDGK
                     VTGAINRLPKQGEIRSNLAAGGKAEAAELTPRELEICDRLGPELARRGLLFVGIDVIA
                     GHLTEINVTSPTGIVAIDRFNGTDTPGLIWDAIEAKVAAYG"
     gene            complement(3457..3807)
                     /locus_tag="VF589_06800"
     CDS             complement(3457..3807)
                     /locus_tag="VF589_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX8257325.1"
                     /translation="MNRRRAERGGRRGETLAALWLQLKGWRILARRARTPVGEVDLVA
                     RRGRVVAFVEVKARASEAEAGHALDDYRLRRVAAAAEALAPRFVRPGDDQRIDAIFIV
                     PRRLPRHLVNVWHG"
     gene            complement(3804..4634)
                     /gene="rsmI"
                     /locus_tag="VF589_06805"
     CDS             complement(3804..4634)
                     /gene="rsmI"
                     /locus_tag="VF589_06805"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX8257326.1"
                     /translation="MSQTLSPGLYIVATPIGNLSDLSPHAAAVLAGADLIAVEDSRVT
                     AKLLAHIGVKRPMTPYHDHNADRVRPGLIAQMAVQAVALVSDAGTPLISDPGYKLVRD
                     ARAAGVSVNTVAGPCAAVAALTLAGLPTDRFLFLGFLPAKSGARAAAIAEVAQIRATL
                     ILYESGPRLGAALAALHAGLGEREAAVVREISKKFEETVTGTLAELAARYAEAPPKGE
                     IVIVVGPPGAAEAAGEAEIDAALREAMTRLSPSRAAAEVAQALGLPRRDVYERALALK
                     "
     gene            4648..>5337
                     /locus_tag="VF589_06810"
     CDS             4648..>5337
                     /locus_tag="VF589_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX8257327.1"
                     /translation="MAEHRHPRQARRSFLGTAAAASLALLMAGCRMIPGDDRPRPPVE
                     PEPLPMPTPTPGPAPEEERNKVAVLVPLSGENAGIGTSISNAANLALLDTGGREIRIT
                     VYDTARGAQAAAAQAVSEGNGLILGPLLAEDVRAVAPVARQARVPVIAFSNDVRVAGN
                     GVYLMGFDPVQSIERVVGHARTQNMQRFAGLAPEGDYGRRSGQALIAAVEGGGGRMVA
                     METYGETSQSLR"
BASE COUNT          812 a         1869 c         1875 g          781 t
ORIGIN      
        1 ggtttcggtc gacatcctcc acgacggcgt cggcacgctc aaggtcgccg tcgccgacct
       61 catcgaccgc aagccggaac ggactgacca aaagggagcg gagcgcctgc cggggaaggt
      121 acgggagcat ctcgaagcgc tcgactgggt ggaaaaggcg gacgtgcgga tgcgcgagga
      181 ggggcatatc ttcgtcggcg aggcgttcgt gatccccaag gcaggcaccc gcgacctcat
      241 cgccaagatc gaacgcgcgg tcgccgaggc gaaggcgatc gactggcgga tgcacgatct
      301 cgtggttcag cccgtggccg atttgccgcc atacaaaccg taagcgcggg ccccgatcat
      361 ttcgccatcg catcttaacc atcatgggag taagctcccg ggcatgggaa cggtgatcaa
      421 cttcgaggac agggcggtcg ccaagctgcg cgcgcgcgtc gccgcggccg aagaggccaa
      481 ccaggatctg atcgcctttg cccgcggcca ttcgggcgcg gtcgcctcga tccacgaggc
      541 ggtgctggcg gcggtcgagg ctgaagggtt cgaccatttg atccacatcg tcactcagga
      601 atggccgcag atactcggcc tcgacgccgt cgccgtcgcc ttgttcgtcg gcggcaaggg
      661 catgcgcgcc gacgcttccg gcctgcagtt cgtcaatccg cgcgtgatcg aacgctcgat
      721 ctcgcaattc gaaggcgtgg tcctgcgcgg ctgcaagcgc ggccatcctt tgttcggccc
      781 ggcctgcgac ctcattcgcg ccgaagcttt ggtccggctc gacagcgacg ccccgcttcc
      841 caagggcctg ctggccctcg gccaacgcgg cgagcagggt ttcgagaccc gccacggatc
      901 ggaattgctc gtcttcctgg ggcgcgtcct gtcgcgttgc atgggccggt ggctgatccc
      961 ttagcggatc atcccgcgcg gcaagaggcg ctgcgctggc aggaccatct cagccaaagc
     1021 cgccggcgct cggcgcatac ggtgcgcgcc tatgccgcca ccgctcaccg gctgatcgat
     1081 ttcctcgccg gctattcggg cgaggcggtc accgccgacg ccctaactcg cctcagggcc
     1141 agcgaccttc gcgccttcct cacccgccgc cgggccgaag ggctcggcaa cagttcggcg
     1201 gcgcgggagc tttcggcggt gcgcgggttt ctcggcttcg tcgcggagca gcagggcagc
     1261 gccgcagcct tgccgcggct gaagggaccg aaaaggccgc gcagcgtgcc gcggccgatc
     1321 tcgcccgccg aagcggtcgc gttggcggag gatgtcgccg aggacgccgc cgagccgtgg
     1381 atcgcggatc gcgattatgc ggtgctgatg ctgctctacg ggtcgggtct gcgcgtcgcc
     1441 gaagcgctcg gcctcaccgg ggcggcgctg ccgctcggtg aggcgctcag cgttaccggc
     1501 aagcgcaaca agactagaat ggtgcctttg ctcgcgccgg tccggcatgc gatcgaggcc
     1561 tatgtcgccg cctgccccta tccggccgcc cgggaagagc cgctgttccg gggcgcgcgc
     1621 ggcgggccgc tcaacggcga gatggtccgc cgcgcggtcc gcaaggcgcg agtccggctc
     1681 ggcctctccg accggaccac gccgcacgcc ctccggcaca gcttcgccac ccatttgctg
     1741 ggccgcggcg ccgacctccg ttcgctgcag gagctgctcg gccatgccag cctctcctcg
     1801 acgcagatct acaccgcggt cgacacggcg cacctgatgg acgtgtaccg caacgcccac
     1861 ccgcgggcgt gaagaacgct agcgccgcgg tttccaggtg acgacgcgga acacatacca
     1921 gagcaccagc acggcgacga ttccggtcga gaccggcccc agatagcggt caacctcggt
     1981 gtaattctgg ccgagcagat agccggccac cgccagcgcg gtggtccagc cgagcgtccc
     2041 gagggtcgac cagaacagga aggcgcgcca gcccatgccg aacaggccgg cggggatcga
     2101 caccagcgag cggatggtcg gcaccatgcg gccgaggaag acgacccagc ggtcgtagcg
     2161 gtcgaacagg gcgtcggcgc gctcgacctc cctgcggtcg agggtcagga accggccgaa
     2221 gcggtcgatc agcggataga agcgttcgat gccgagcgcg cgggccaccc aataccacat
     2281 gatgttgccg agcatcgcgc ccagggtgcc ggcgaggatc gcgccccaga gcgtcatcgt
     2341 cccgcgctgc gcctggaggc cggacagcga catgatcacc tccgacggga tcggcgggaa
     2401 cacggtctcg agcagcatca gcaggcagac gccgacatag cccgattgct cgatcagctc
     2461 gcgaacccag tcacccataa gctgccactt tcgcttcgat cgcgtcccag atcagccccg
     2521 gcgtgtcggt gccgttgaac cggtcgatgg cgacgatccc ggtcggcgag gtgacgttga
     2581 tctcggtcag atggccggcg atcacgtcga tcccgacgaa caggagccct cggcgcgcca
     2641 gctcggggcc gagcctgtcg catatctcca gttcccgcgg tgtgagttcg gccgcttcgg
     2701 ccttgccgcc ggcggcgagg ttggagcgga tctcgccttg cttggggagc cggttgatcg
     2761 ctcccgtcac cttgccgtcg accagcacga tgcgcttgtc gcctagggac acgtcgggga
     2821 ggaaggcctg gaccatgaac ggctcgcgcc agacctcgcc gaacaattcg gtgagcgcgg
     2881 cgaggttggc gtcgctgcgc ccgacgtgga agacggcggc gccggcattg ccgtagagcg
     2941 gcttcaccac gacttcgcca tgctcggcgt ggaaggcccg cgtctcgtcg aggctgcggg
     3001 tgatcagggt cggcggcatg aactcggcat aatccagcac gaacagcttt tcgggcgcgt
     3061 tgcggaccga tgcgggatcg ttgaccacca acgtgcgcgg atggatgcgc tccaaaaggt
     3121 gggtggcggt gatgtaggcg aggtcgaacg gcgggtcctg ccgcatcagc acgacgtcgg
     3181 cgtcttcgcc caggtcgagc cggaccggtt cgccgaaccg gaaatagtcg ccctccaccc
     3241 gctgcacgct caccggccgc gccagggcgc tgacccggcc ggcgcgatag ctgaggtcgc
     3301 cggcctcgta atggaacagc cgatggccgc gcgcctcggc cgacagcatg atcgcgaagg
     3361 tcgaatcgcc cgcgatgttg atcccctcca tggggtccat ctggacggcg acgcgcaaac
     3421 tcatggcgct gcccctagcg gcggtccctt ggcttgtcac ccgtgccaga cgttgacgag
     3481 atggcgcggg aggcggcgcg gcacgatgaa gatagcgtcg atccgctggt cgtcgccggg
     3541 tcgcacgaag cgcggcgcca gcgcctcggc ggcggcggcg acgcggcgga gccggtaatc
     3601 gtcgagcgcg tggccggctt cggcctcgct cgcgcgcgcc ttcacttcga cgaaggcgac
     3661 cacgcgcccg cgccgcgcca ccagatcgac ctcgccgacc ggcgttcgcg cgcgccgcgc
     3721 gagaatgcgc cagcccttga gctgcagcca gagcgccgcc aaggtctcgc cgcgccggcc
     3781 gccgcgctcg gcgcgccggc ggttcatttg agcgccagcg cccgctcgta gacgtcgcgc
     3841 cgggggagcc ccagcgcctg cgcgacctcg gcggcggcgc gcgagggcga cagccgggtc
     3901 atcgcctcgc gcagcgccgc gtcgatctcg gcctcgccgg cggcttcggc cgcgccgggc
     3961 gggccaacga cgatgacgat ctcgcctttg ggcggcgcct cggcatagcg ggcggcgagc
     4021 tcggcgagcg tgccggtgac ggtctcctcg aacttcttgc tgatctcccg caccaccgcc
     4081 gcctcgcgct cgccgagccc tgcgtggagc gcggccagcg ccgcgcccag cctcgggccg
     4141 ctctcataaa ggatcaaggt cgcccggatc tgcgccacct cggcgatggc ggcggcgcgc
     4201 gcgcccgact tggcggggag gaagcccagg aacaggaagc ggtcggtcgg cagccccgcc
     4261 agagtcagcg ccgcgaccgc cgcgcacggg ccggcgacgg tgttgacgct cacccccgcg
     4321 gcgcgggcgt cgcgcaccaa tttgtagccg gggtcggaga tgagcggcgt tcccgcgtcg
     4381 gacaccaggg cgaccgcctg caccgccatc tgcgcgatca gccccggccg cacgcggtcg
     4441 gcattatgat cgtgataggg cgtcatcggc cgcttgacgc cgatatgggc gagcagcttg
     4501 gcggtgacgc ggctatcctc gaccgcgatc aggtcggctc ccgccagcac cgccgcggca
     4561 tgcgggctga ggtcggaaag gtttcctatc ggggtcgcga cgatatagag gcccggcgaa
     4621 agagtctggc tcataaggga catcgtcatg gcagagcatc ggcacccgcg gcaagcgcgg
     4681 cgctccttcc tcggcacggc ggcagccgcg agcctcgctt tgctgatggc cggctgccgc
     4741 atgatcccgg gcgacgaccg gccgcggccg ccggtggagc ccgagccgct gccgatgccg
     4801 acgccgaccc ccggtccggc cccggaggag gagcgcaaca aggtcgccgt gctggttccg
     4861 ctcagcggcg agaatgccgg catcggcacc tccatctcca acgccgccaa cctcgccttg
     4921 ctcgacaccg gcgggcggga gatcaggatc accgtctacg acaccgcccg cggcgcccag
     4981 gccgccgctg cccaggccgt gtcggaaggc aatggcctga tcctcggccc gctgctcgcc
     5041 gaggacgtgc gcgcggtcgc gccggtggcg cggcaggcgc gggtgccggt gatcgccttt
     5101 tccaacgacg tccgcgtggc cggcaacggc gtctatctga tgggcttcga tccggttcag
     5161 tcgatcgagc gggtggtggg ccatgcgcgc acgcagaata tgcagcgctt cgccggcctc
     5221 gctcccgagg gcgattatgg ccgccgttcg ggccaggcgc tgatcgccgc ggtcgagggc
     5281 ggcggcgggc gcatggtggc gatggagacc tatggcgaaa cgtcgcaatc cctgcgc
//
LOCUS       DASVRK010000176        38997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_33281, whole genome shotgun sequence.
ACCESSION   DASVRK010000176 DASVRK010000000
VERSION     DASVRK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 38997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38997
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33281"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VF589_06815"
     CDS             complement(<1..363)
                     /locus_tag="VF589_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257328.1"
                     /translation="MTMDLLIELAWKSVLCAGAALALLRLFRSRSAAEKALVAHLGLV
                     ALILLPLASLWLPSIELPAPGAVAEALPMLAPAEPAPLAMAAPAVEAAPRIDGAAATF
                     AAYFVPLLVLLAALAVAVL"
     gene            complement(360..749)
                     /locus_tag="VF589_06820"
     CDS             complement(360..749)
                     /locus_tag="VF589_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX8257329.1"
                     /translation="MIETLPPRERQVFDALFASGEASAAELEARMADPPSNSALRIML
                     SRLEKKGFVTRRLVDQKYLYAPALPQRKVKQSALRHLIKTFFDGSPVGAAAALIGMQE
                     RIEPEELDELERLIAEARREQGLEKGA"
     gene            843..1589
                     /locus_tag="VF589_06825"
     CDS             843..1589
                     /locus_tag="VF589_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8257330.1"
                     /translation="MASSFAPLRSGWMGAIIGTAGWSIPASEAGHFPADGTALERYAA
                     RFGGVEINSSFHRPHRASTWARWGESVPAGFRFAVKVPKTITHARKLVDCGELAAEFV
                     EEAQGLGDKLAILLVQLPPKLAFDEALAQRFFEHLSGLTPATIVCEPRHPSWLEAAAD
                     ALLDSLGVARVAADPAILPAANVPGGWRGLSYWRLHGSPAMYRSAYDDARLDDYARQI
                     GAEPGQAWCMFDNTAASAATGDALKLMARL"
     gene            1586..2323
                     /locus_tag="VF589_06830"
     CDS             1586..2323
                     /locus_tag="VF589_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8257331.1"
                     /translation="MTEREDRHGPSAAVAWLVHGFTAMGAVLAFLALMAILEEDWALA
                     LGWLLAALVVDGVDGSLARWAKVKARAPRIDGETLDLVVDYLNYVFVPAVLVWRAGLV
                     PERAAPWLCGLILVSALYNFTRRDLKTEDNYFRGFPALWNVVAFYLFVARPGPEAGAA
                     AVILFAALTFAPVHFVHPFRVSDYGRWLPALATAWAAATAALLWPGWNEEARSAWLAV
                     SLGAAAILLALGLARTVRGQRPERLRR"
     gene            complement(2313..6059)
                     /locus_tag="VF589_06835"
     CDS             complement(2313..6059)
                     /locus_tag="VF589_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257332.1"
                     /translation="MTVAYFRETPGVAIDGNTANASVDAVELADGRQIIVWADQVEAT
                     GAPVIRGQFLDAQGAPAGEPVNLVEGYSVVDDLVATSAGFTLSYGVPYAGQLRAFNFN
                     GAPVSPSVKLSGPYAQLTTLSDGNVLAIWGENDAGPGERLMGQLFSAEGLALGAAELL
                     VAEVYGSLDVVALADGAFVVAYSSDIEWDDFVDGELTRITTRRFDGFGEAGDASIFHG
                     NPETSDSYGNDRAGKWLNCFGPSLAATPTGYALVWTESFYESFGAGEPEPPYESSRVR
                     GRVIDQGRAYPEFDVSSAESRGVPAELSAGPDGSLFVAGAAGDDSESRTATGQALASD
                     GTLIGEQIAIGEPNARFRPDIALGRLADGDLFAIVAGRSPTGAGSFMVDLQRLTAHDF
                     ASVFRGSAGEDVAAGTELADYLVGLAGNDRLSGGGGDDHIGGGAGDDLLDGGGGGDRI
                     NGGDGDDLLGGGGGDDHIDGGDGNDALDGGDGDDRVYGGGGNDLLDGGGGVDRLAGGA
                     GDDRYIQGAGGSIVELAGQGTDTLVASTDVTTLTDEVENLDLTGGAIEGGGNDGHNVI
                     TGNSLDNRLYGGGGYDRLSGGGGDDRLDGQAEHDLLLGRAGADLLISLEGEDRLFGGS
                     GDDRYELGAGSLARVIEGGGEGFDTIVAAASHRLSAHVEALELTGSAYFGYGNDGDNI
                     ITGTSGRNRLDGGRGADELIGLGGNDTLFGADLGEAADRLDGGEGDDVYHVDLIDVIV
                     DAGCRDRVIVAGSYALQEGLETLVLAGAGDANGTGNSGSNLILGNGGDNGISGLGGND
                     TLRGAEGNDELSGGAGNDRLDAGAGADLLEGGAGNDRLAGGEGEDTGSWAGAAAAAAV
                     DLLVTEAQITGDGRDVLSGIEHLVGSAFSDLLSGDLIANRIAGGDGNDRLRGRDGNDV
                     LAGGGGDDALSGGNHDDGLSGGDGQDFVSGQNSDDLIDGGTGDDRLFGGDGADRMDGG
                     GGGDAMDGGTGDDIYVVDDAADRVIEQVGGGDDVVLAGVDHILAAEVEQLRLTGAARA
                     GTGNGLANLINGSDLDNAIAGEGGDDFIFGRSGDDRLAGGDGRDTIDAGAGRDDVSGD
                     GGEDRLSGGSEADRIDGGSEADRLFGDDGRDLLIGGGGDDLLYGGFDRDTLRGGEGAD
                     GFVFREGETGGSALTADCIADFAGAEGDRIDLHRIDASFSGAGDQAFDFIGDADFSGV
                     AGELRAFADGGYTYVEGDRDGDGAADIMLCLTGEQALAATDFIV"
     gene            6304..7920
                     /locus_tag="VF589_06840"
     CDS             6304..7920
                     /locus_tag="VF589_06840"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX8257333.1"
                     /translation="MSPFTSRAGYSFWIKAAMALLLVVLGDLLFFRQWLGATLGLFAI
                     AWLAGLLAAAPAAMRKQPSALAALAALAFALVMIEDPSPLAWGAFWCALSLAALLPRM
                     ARFGAASQMAVRLFLHGLSAIIAPWSDLNRLRRLRPSERRPRIRSLLPLLPLPLAGGA
                     LFVGLFSIANPLIANLFTAVELPPPNLDRVRIALWIALAGMVWASLRPRAGVTRPWDA
                     HVIDRGPLPGVSVASVTLSLLLFNLIFAVQNGLDIAFLWSGARLPGAMTLAGYAHRGA
                     YPLIATALLAGLFVLVTLRPGSETAARPLIRRLVVLWIGQNIFLVASSVLRTLDYIDA
                     YSLTRLRIAALAWMLLVALGLALICWRMLKERSGTWLINTNAAAAAAMLSLATIVDLG
                     AVAAHWNVRHAREVGGRGTHIDLCYLNQLGASAVLPLIELEARPLPPALRDRVAWVRI
                     GAMDGLETRQADWHSWTWRNARRLAAARAAEPAPVTLPQAPFGRKCDGSVWTPLPVPY
                     PAGTRSPSPALPSPAPAAPAEPRLTAEQGR"
     gene            7932..8639
                     /locus_tag="VF589_06845"
     CDS             7932..8639
                     /locus_tag="VF589_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8257334.1"
                     /translation="MPRTILVADDDPHIRQLLVFALAKAGLAAIEAEDGEAALAAARE
                     QSPDLVILDINMPRMDGLEVCRRLRADGGMPVLFLSSRDDEIDRVLGIELGADDYVTK
                     PFSPREVVARVQAILRRIAAHPPAVEGAFPAIRRGLLTLDIDGWQARWGEAEVPLTVT
                     EFGILRTLAGMPSKVFTRDAIIDRLRGPGFALTERTIDSHVRNLRAKFAQAGGGDVID
                     TRAGVGYRLGPCGGGSG"
     gene            8636..10204
                     /locus_tag="VF589_06850"
     CDS             8636..10204
                     /locus_tag="VF589_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8257335.1"
                     /translation="MTGGVKALIKRYWPRLRLRTILLATLLFVAALPGVGALFLRVYE
                     NTLVRQTESELIAQGAALAAAAEAAWPGSALPAARRLRPDEYRPEPPQIDLNGTPILP
                     ERPAPARGSGLDPAAVAAAGRIAPVMMRTAQTTLASIQLLDRRGTVVTGPLAGASYAR
                     LPEVAGALAGRPTTVLRHNGSYRPEYPLEWLSRASDLRIHHVRPVSAGGQVVGALLLS
                     RSPRALFRGLYEDRGKILFGIAAIFATLVVLSGLLSRGISRPIERLSAATRRLALGGG
                     GEVPEPPQTAAVEIQALYRDFADMAEAIERRSRYLRDFAHAVSHEFKTPLAGIRGGIE
                     LLQDHQASMAEAERERFLANVAADAERLAQLVSRLLQLARADMARPDPDAATAPAALR
                     SLVDAAGGRGIALRGDLPEDLPALAVPPATVEAALATLADNSLQAGATAIEIVARAEA
                     DALVMEIGDNGPGVPPSDRERIFEPFFTSRRSAGGTGLGLPIVRSLLEASRATIELVP
                     SAAGARFRIRLPLA"
     gene            complement(10223..10669)
                     /locus_tag="VF589_06855"
     CDS             complement(10223..10669)
                     /locus_tag="VF589_06855"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX8257336.1"
                     /translation="MESVIRALCTYFFLLLVFRIAGRRSLAEMSSFELVILLIISETT
                     QEAMIDSDPSMTNAGLAIITLVAAGIAMSVLKERWPRFQTIVSGRPVLLIEDGQVMRD
                     RLSEARIGLDEIMVAARANHGLERVGQVRHAILEAGGGISIIPYER"
     gene            complement(10683..10823)
                     /locus_tag="VF589_06860"
     CDS             complement(10683..10823)
                     /locus_tag="VF589_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257337.1"
                     /translation="MKAPRLGGRETVILYHYQADGPFADIPARIGIEGGPEVRFNQFV
                     YP"
     gene            complement(10820..11200)
                     /locus_tag="VF589_06865"
     CDS             complement(10820..11200)
                     /locus_tag="VF589_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257338.1"
                     /translation="MAAAEKTEDAGLEIPEGSGVTPREERFHRVGWLLLALLVLAALA
                     GLLGPGPLSSRTARAGPALSVEYERFVRNHAPADLRIRVVPPPGAGVIRLHVNRAFLE
                     ATQLDNVAPEPEAVEIGPAGIPTS"
     gene            complement(11265..11831)
                     /locus_tag="VF589_06870"
     CDS             complement(11265..11831)
                     /locus_tag="VF589_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX8257339.1"
                     /translation="MEIFETALGLDRQSMDLTIGQMALRAAVTYAVTLAIVRLGKKRF
                     MGSNSAFDVIVGIILGSTVSRGLTGNAPLGPTLAAAAAIVAMHWLVSGLALHWRGFSR
                     LAKGDARLLVRAGTPDEAALRKVHMTQGDLDEELRGHGHADASRVAEARIERSGRVSI
                     IEAAGEDRILEVAVADGVQTIRIQLARG"
     gene            12178..16341
                     /gene="rpoB"
                     /locus_tag="VF589_06875"
     CDS             12178..16341
                     /gene="rpoB"
                     /locus_tag="VF589_06875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX8257340.1"
                     /translation="MATIAPQAGELRSTASKRIRKIFGNIHEVSEMPNLIEVQRESYE
                     QFLRSRPQDGYVSGLEKTLRSVFPIRDFAGTAELDFVNYELEDPKYDTDECRQRGMTY
                     AAPMRVTLRLIVFEVDPDTETRSVLDIKEQDVYMGDMPLMTKNGTFIVNGTERVIVSQ
                     MHRSPGVLFDHDRGKTHASGKYLFAARVIPYRGSWLDFEFDAKDIVNVRIDRKRKLPV
                     TALLHALDMSSEEILNTFYNTVTFVRAPNGWQIPFVAEQWRGQKPSFDLANGETGEVV
                     FPAGQKITPRGANKAAKDGLSRLLIPTEEIFGRYSAFDLVNDSTGEIYIEAGDEVSPE
                     NLERLDRAGIDRIELLDIDHVNTGAWMRNTLKADKAEDRDQALSDIYRVMRPGEPPTR
                     ETADALFAGLFFDPERYDLSAVGRVKLNMRLDLDVEDTVTTLRKEDIIAVVKTLVDLK
                     DGKGEIDDIDNLGNRRVRSVGELLENQYRVGLLRMERAVKERMSSVDVSTVMPNDLIN
                     AKPAVAAVREFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLTRERAGFEVRDVHP
                     THYGRICPIETPEGPNIGLINSLASFSRVNKYGFIETPYRKVVDHKVTDDVVYLSAME
                     EAKHTIAQANAELSDDKHFVEEIVSSRQAGEFLMAPRDIITLMDVSPKQLVSVAASLI
                     PFLENDDANRALMGSNMQRQAVPLVRAEAPFVGTGMEETVARDSGAAIAARRSGIVDQ
                     VDATRIVVRATGEVNAGTSGVDIYTLMKFQRSNQNTCINQRPLVKVGDVVNAGDVIAD
                     GPSTEFGELALGRNVLVAFMPWNGYNYEDSILISERIVKDDVFTSIHIEEFEVMARDT
                     KLGPEDITRDIPNVGEEALRNLDEAGIVYIGAEVEPGDILCGKITPKGESPMTPEEKL
                     LRAIFGEKASDVRDTSLRLPPGVAGTVVEVRVFNRHGIDIDDRTRAIQTEEKDRLRKD
                     ADDERSILKRATYSRLREMLLGQTATAAPKGVKKGVAIDEATLESVERHEWWKFAVKD
                     DAVQANLEAVKGQYDEAEAVIRRRLEDRIEKLERGDELPPGVLKMVKVFVAVKRKLQP
                     GDKMAGRHGNKGVISRILPIEDMPFLEDGTHADIVLNPLGVPSRMNVGQIFETHLGWA
                     ARGLGKQIGQMLEDIHGKGKALEGADVTNVRNKLKQIYGENYFADLDARTDEDLMELA
                     SNLVAGVPMGTPVFDGAREANVSEMLTMAGLDPSGQVTLYDGRTGEAFDRKVTVGYIY
                     MLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALQAYGAAYTLQE
                     MLTVKSDDVIGRTKVYEAIVKGDDTFEAGIPESFNVLVKEMRSLGLNVELNSVIDPDA
                     DDQQQLAAE"
     gene            16490..20758
                     /gene="rpoC"
                     /locus_tag="VF589_06880"
     CDS             16490..20758
                     /gene="rpoC"
                     /locus_tag="VF589_06880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX8257341.1"
                     /translation="MNELSNFANPVVKPETFDQIQIGIASPERIRSWSFGEIKKPETI
                     NYRTFKPERDGLFCARIFGPIKDYECLCGKYKRMKYKGIVCEKCGVEVTVSKVRRERM
                     GHIELAAPVAHIWFLKSLPSRIGLLLDMQLKQLERVLYFEAYIVIEPGLTPLEKYQLL
                     TEDELLEAQDEYGEDAFSAGIGAEAVRVMLENLDLEGEREELLKELAETKSELKPKKI
                     IKRLKVVESFLESGNRPEWMILEVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLMELRAPDIIVRNEKRMLQESVDALFDNGRRGRTITGANKRPLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVTGPELKLHQCGLPKKMALELFKPFIYSRLDAKGLS
                     MTLKQAKKWVEKERKEVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNILSPANGKPIIVPSQDMV
                     LGLYYLSMEREGEPGENMMISDMSEVHQALNAKAVTLHSKIVSRVPQTDEQGKTYMKR
                     FETTPGRMLLGETLPKSHKVPFETVNRLLTKKEIGDVIDVVYRHTGQKETVLFADAIM
                     GLGFRHAFKAGISFGKDDMIIPHEKVGMVDETRALVKDYEQQYQDGLITQQEKYNKVI
                     DAWSRCGDQVANAMMEKIKAQPKDENGRMAPINSIYMMAHSGARGSQAQMKQLAGMRG
                     LMAKPSGEIIETPIISNFKEGLTVLEYFNSTHGARKGLADTALKTANSGYLTRRLVDV
                     SQDCVIVEEDCKTENVLDMRAIVQGGAVIASLGERILGRTPGEDIVDDKTGTVLAKEG
                     ELIDEAAATRIDEMGIQSVNIRSPLVCESEHGVCGKCYGRDLARGTPVNIGEAVGVIA
                     AQSIGEPGTQLTMRTFHIGGAAQLNETSSLEASTDGTIELRDLPTILDARGRRVAMAR
                     NGELAILDMDGRERAVHRIPYGAHLMADHGHIVSRGDRIAEWDPFMMPVITEKGGVVK
                     FQDMVEGKTLTEQVDEATGIAQRIVTEFRGSARSKEDLRPRITLLDEESGEAARYMLS
                     PGAMLSVEDGQQIGAGEVLARVSREAAKTRDITGGLPRVAELFEARKPKENAIIAKVS
                     GRVEFGKDYKAKRKIGIRPEDGGELVEYLVPKSKVIDVQEGDFVKRGDNLIGGSPDPH
                     DILEVLGVKALAEYLVSEIQEVYRLQGVKINDKHIEVIVRQMLQKVEITESGDTTFLV
                     GEQVDHQEMREANARLTNKQRKAEGKPVLLGITKASLQTRSFISAASFQETTRVLTEA
                     SVQGKIDTLDGLKENVIVGRLIPAGTGAGMNRMRIAATSRDAALRAQHRKVQASLIAA
                     NSAEEEHAAELAQGPEAAIGDDPLGEVASVGHGTDADAGEYLQT"
     gene            21021..22199
                     /locus_tag="VF589_06885"
     CDS             21021..22199
                     /locus_tag="VF589_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8257342.1"
                     /translation="METSDLRIAMFSGNYNYVRDGANQALNRLVDYLAKRGAAVRVYS
                     PTVDDPAFPPNAPVVKVPAFPIPGRSEYRAPFMLPRRAKKDLRAFRPNILHIASPEVL
                     GHRAVTLAHKLDLPVVASVHTRFETYPRYYGLSVLEPLVEAALRRLYRRCDAIFAPSD
                     SMAQLLREQRMNYNVGIWTRGIDREIFNPARRDLEWRRGLGIDDDVPVIGFVGRLVME
                     KGLDVFSETVDTLERRQVRHKVLVVGEGPARDWFEKRLPNGIFAGFQEGPDLGRAVAS
                     MDMLFNPSVTETFGNVTLEAMAVGLPVVAAIATGSESLVEDRVTGRLIRPGAIEAFAD
                     ALARYCGNEEERIAAGQAGARVSLRYGWDEVNQSLVDAYIRIIRQRAAGARMKRSPVP
                     "
     gene            22228..23658
                     /locus_tag="VF589_06890"
     CDS             22228..23658
                     /locus_tag="VF589_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX8257343.1"
                     /translation="MSDLFGDQAAAPAPPPPDTAPLADRLRPRSLDEVVGQEHLTGPE
                     GAIARQVAAGKLASMVLWGPPGTGKTTIARLLADAVGMRFVAISAVFSGVADLKKVFA
                     EAKEHVRAGRQTLLFVDEIHRFNRAQQDGFLPYVENGTVVLVGATTENPSFELNAALL
                     SRAQVLILNRLDARALGKLLDRAEALIGRPLPLTPEARDALVATADGDGRFLLNQAET
                     LFSISLSPSGERVPSEAKAGEGEFGGQDADGLPLSPTLSPGGERGNLLDAAALSALLH
                     RRVAVYDKDREGHYNLISALHKAIRGSDPQAALYYLARMLVAGEEPLYLLRRLTRAAV
                     EDIGLADPQALVQCIAAKDTYDFLGSPEGELAIVQACLYLATAPKSNATYMAQKAAWR
                     SAKETGSLMPPKNILNAPTKLMKEVGYGRGYTYDHDAPEGFSGDDYWPEEMEPQAFYR
                     PTDRGFEKRIAERIAWWEERRAELRG"
     gene            23744..25123
                     /locus_tag="VF589_06895"
     CDS             23744..25123
                     /locus_tag="VF589_06895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8257344.1"
                     /translation="MTRVATCLALVALAAPGAAQVDSAPYTRAIAAGYKASFLCSDIF
                     NAGQSEAIVEQDDLQRTYAELVPIMRNLKPVIDRQAKTVSVAFDDKLPPRIASWRPHL
                     GCAQLPIGARMESRSALPSLSVDPAVDRSDRLPWPTGDRNAEGKPRGDAAALRQLVAA
                     AFDRRSYGQGSETTALVIVQDGRIVAERYRADFTRHTSQRTWSVAKSIAGTLAGVAVQ
                     QRRLDVDAPAPIPEWQSPGDPRAAITTDQLLRMASGLHSDHAGNRTDALYFGGTAVTE
                     QATGWPLEAAPGTRFRYANNDTLLAVRGLRSALGEERYLSFPFTELFWKVGMTRTVPE
                     TDWKGNFILSSQVWSTARDLARLGLLYLQDGVWNGERILPPGWDDYVRRHGPAQPAAG
                     GGYGATFWTFPTGKGLPEDASVMRGNRGQYVVIIPSRSLVIVRRGYDGAESQFDLDRF
                     TADLLAALR"
     gene            25210..26499
                     /locus_tag="VF589_06900"
     CDS             25210..26499
                     /locus_tag="VF589_06900"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEX8257345.1"
                     /translation="MYQSDPGSLYWQGQVQEWLPRIVGGLAILIIAYILARAAKWAIA
                     KVVDRVPALKKHHEAEPGKTLGSLIGDIAFWLILLIGIMLALQPLGLSQVLEPVRQLT
                     TNVFAFLPKLLAAGLIFFVGMVIAKIVRRLVEGALLAANADGWLRRTGLVETAGTAPA
                     TPAGGPVTTTSRASISRSVGTLVFFVIMIPVAIMALDTLQLEAISGPATQMLRTILDA
                     IPLVLGALILLAIGYFVGKLAKQAIEQILPSMGFDRAVGALGLSPGSTTPSRTVGTIV
                     MLAILLFFAVKAAELLQSPIIAVMLAQLLELGGRVLFGAGIILGGFVFAKIASNLVAG
                     TGSEGWLPQILKWSIIALSVAMGLRFMGLANEIVIIAFASIIGSAAVACALAFGLGGR
                     PTAHKLLERWTEANQVPAPPRRPRTPTPPDGTQPPLV"
     gene            26542..27429
                     /locus_tag="VF589_06905"
     CDS             26542..27429
                     /locus_tag="VF589_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257346.1"
                     /translation="MTAAPTPFTRFAAIDWSGAKGRRHKGIAVAVCEAGGGAPRLVQR
                     AAPWSRTEILDWLLATAAEAPTLYGFDFSYAPPIVERGAYLPGEAGPPSDAKSFWAYV
                     DGLCEDEDLGAASFMERHHRRHFYFGAADGVKADFLHHRVCEHAFNALGGGKASTLYD
                     AIGAAQVAKASYAGMRLLHRLGGRVPVWPFDFAQDRPMDPVPAAGSLVVEIYTRVFIR
                     LAGLSGRKVRSRGLLDEALAGLGSGPASLAGEPNDHETDVIIAAAGLRRIADDPLYWS
                     PPALTPAIARTEGWTFGVL"
     gene            complement(27446..27521)
                     /locus_tag="VF589_06910"
     tRNA            complement(27446..27521)
                     /locus_tag="VF589_06910"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27486..27488),aa:Thr,seq:tgt)
     gene            27668..28303
                     /locus_tag="VF589_06915"
     CDS             27668..28303
                     /locus_tag="VF589_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257347.1"
                     /translation="MEEELAEELIAVEAEPGDEEVWAEELPVEAAESSDGEGGGSDEI
                     PADEEIYTLYLEDEVPAEDAGDGSDGEEELPADEEIYTTYIDEGEVPADEGPGEDPGN
                     LEDPVVDEGGTEVVDGEGKEIGSVDGEIDYIGGGWDELLYPTMNEGEWAYTTSAGGID
                     DPRIYMSSGSGPAPNERGEEAAQVVEVATWHTVNLVHHALVADLAPREHIA"
     gene            complement(28385..29548)
                     /locus_tag="VF589_06920"
     CDS             complement(28385..29548)
                     /locus_tag="VF589_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8257348.1"
                     /translation="MPAFTMPVLRMPSLERKHWGATIVLATLACLYLLAGLSVVGLGG
                     PDGRALARVSGNLPEAVRKTLPQAEPLQFKQVAPTDAVAINAAVPLSAAANPAATAFA
                     LLARTESDRARALQCLTAAVYYEAATEPLDGQRAVAQVVLNRVRHPAYPSSVCGVVWQ
                     GSERSTGCQFTFTCDGSLARTPMASVWARAGQVAQAALAGYVYAPVGHATHYHTNWVV
                     PYWSSSLEKIANVGTHIFYRWSNGWGRPEAFRHRHSGVEPVVSMTARKAQDPAALAAA
                     GATDALAAEAAASAAAGAGGSASVDSFQRAVLRRYVPADRSGVATVLAAQTKSGETVA
                     KSHLWALTGNSSGGGAAEAPLGAKAEASPAKPACIEGVKKAPAEGAAPAPLTC"
     gene            29830..30723
                     /locus_tag="VF589_06925"
     CDS             29830..30723
                     /locus_tag="VF589_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8257349.1"
                     /translation="MATQARVPAHVEPAQFLTDSFQRALAHWNHARLAPQFPGADWQK
                     ALDRDVQMLRLEGGLLEGLRAEVVAEAAAAPTDADGFIAWFEALKDSGPGQGDPLFPW
                     LAGEASLEEMRWFLTQEAAGEAGFDDLVAYTQVKLPTRAKLELARNYWDEMGRGNPKG
                     MHGPMLEVLIDTMAIEPDIDTTAWQSLALANAMTAMATSRRYAWHSVGALGAVELTAP
                     GRSAATAAGLRRLGFSGRDRRYFAVHAVLDVKHSEDWNREALRPLVEEDPRRATAIAE
                     GALIRLNCGARCFERYRAELW"
     gene            30988..32103
                     /locus_tag="VF589_06930"
     CDS             30988..32103
                     /locus_tag="VF589_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8257350.1"
                     /translation="MATIAPPAPPPLLLPGDHAPWFHAPALSGSPNYAFQTVAGRHVM
                     MLFLGSTRIEACAAALRLVLERRAMFDDVNACFFGVTVDREDEAKGHIAQLLPGLRFF
                     LDYSQEVSRLYGAVGKAGEGNVEYRPHWLLLDPGLRVVDRFPIDRGGEALDALQHAIA
                     APAGPAWAPVLEVPNVLEPELCHELIALYEGEGGTESGFMRDVDGKTVLIVDAAHKRR
                     SDCMIEDEALKRRLVARMNRRLSPALKKAFQFEATRIERYLVACYDASGGYFRPHRDN
                     TTKGTAHRQFAVTINLNAGDYEGGDLRFPEYGQRTYRAPTGGAIVFSCSLLHEATPVT
                     SGRRYAFLPFLYNEEAARVRESNNPYLGEGIGEYRIG"
     gene            32122..32589
                     /locus_tag="VF589_06935"
     CDS             32122..32589
                     /locus_tag="VF589_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX8257351.1"
                     /translation="MSLESVRADLAARAPDVPIIEMGQSTATVELAAQALGVEPGRIA
                     KTLAVRVGAETFLLVARGDARLDNQKTKAAFGGRPRLLGPEETLALTGHPVGGVCPFG
                     LATPLPVYCDLSLQAYDLVYPAAGSLESSVKLSPARLVELVGGRWVDLCRLPG"
     gene            complement(32600..33988)
                     /locus_tag="VF589_06940"
     CDS             complement(32600..33988)
                     /locus_tag="VF589_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX8257352.1"
                     /translation="MDDVLFDIAQERALDYIAKAHRRRVFPDAAAVAGLAAFDEPLPD
                     RPVDALDTIRLLDRAGSPATVATTGGRYFGFVTGGSLPVATAADWLAAAWDQTGTMPV
                     SSPAATRIECVAGRWVVEMLGLPPESVTGFVTGASIGNLVGLAAARRHLLLRLGHDVD
                     ADGLAGAPRLRVVAGEEVHSTVLKMLGILGFGRGNVELVPVDGQGRLRADRLPALDDR
                     TILILQAGNVNSGASDPFVPAIAAARAAGAWVHVDGAFGLWAAASPRHRHLVEGVEGA
                     DSWVTDGHKWLNTPYDSGMIVCRHADALRGAMGLAATYMPASEEVPMKDLVPDFSRRA
                     RGIIVWAALRTLGREGVAELVERCCGHAGRLAEGLRAIGFEIHNEVVLNQVVASIGDE
                     DFTARVRDQVERGGECWFGPTHWQGRAAVRLSVSSWATTDADIDRSLAAIGKAVTAVR
                     SRRRVEAAPSVA"
     gene            complement(34009..35049)
                     /locus_tag="VF589_06945"
     CDS             complement(34009..35049)
                     /locus_tag="VF589_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX8257353.1"
                     /translation="MTDLFTPVRLGRYALRNRIVMAPMTRDRSPGAVPTELVRTYYAQ
                     RATAGLIVTEGIAPEPMGHGYVDSPGLYTDAQVRAWQRVTDAVHARGGRIFAQLMHVG
                     RISHPALLDGRTPVAPSTVGGADHVVPLALEAGEISNIVAGFARAARNAVRAGFDGVE
                     IHGANGYLPHQFLATNVNLRGDEWRGSIEKRARFLIEVAAAAAAAIGADRVGVRISPG
                     NGYNDIVEADAAATYAYLLDRLDALGLAYLHVYDTRPGFDVAELVLGHYRGRLILNGG
                     YDKAGAEADLAEGRADLIAFGTAFIANPDLPARLASGAPLAEADRATFYSSGARGYTD
                     YPALAGTLAEAA"
     gene            complement(35066..35776)
                     /locus_tag="VF589_06950"
     CDS             complement(35066..35776)
                     /locus_tag="VF589_06950"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8257354.1"
                     /translation="MAQQSDIGIIGTGRMGVRLAAMFARAGRTVTLGSRSLRRATYIA
                     EALNLDGLRPGSYEDAAAAGAFLPAVFLRDGLLDQVGPLAKRLRGKLLIDISNPFNED
                     YSDFITPWDSSGAEELQKRLPETRVVGAYKNVFWEVFDRPLFEEGASDVLVTGDDAAA
                     KRELLGLAEGTPFRYLDAGPLRNARAIERMTLITGALGRQIGAYPRMNWRLLGSRGWT
                     PGQDLQGIDRLIAAPLAA"
     gene            complement(35837..36718)
                     /locus_tag="VF589_06955"
     CDS             complement(35837..36718)
                     /locus_tag="VF589_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8257355.1"
                     /translation="MDLNQTRVFVEVVRAGGFAAAGRRLHLPKSTVSARVQALEARLG
                     AQLLKRSTRQVALTSEGRAYFDAVACAVDALVDAEAATTAEDGVLSGPIRFTAPLEFP
                     RDALTTAICSFLENHPKVQFEITLTNRPVDLVAENIDLALRGGDPGGAGLIIRKVGQI
                     VFGLYASPSYIARHGRPADLAALVDHNLLLFSSPSQSRVLRAADPVGAGAARISCDNF
                     AFLRRLAARGAGITALPDALVTPQVASGALVRVLDGWNSEPSALHLVFPSRRDMTPRV
                     KAFADHLAAALGDSPIA"
     gene            complement(36822..>38997)
                     /locus_tag="VF589_06960"
     CDS             complement(36822..>38997)
                     /locus_tag="VF589_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257356.1"
                     /translation="AAPASGEAYARPGSLAIFWDRSLSRADDDHGRELALLRRYLQRV
                     RPARIELILFDSGGAERLSLGADEVVARLKTVRYRGATSFAVIGGGAPADACLLFSDG
                     LATIDRRGDFRPACPLSAISAAADADRAWLRDIARGGGAYDLIGTSADHVLESLTRAV
                     PRVAGVRSASGQPVEFVQLDAAEGGWRIVGQAPEGESLIVDLAGVEAGAAQRRYPLAG
                     GAVPTLAGAGSLWAADRAALRAASDALDREALIAFSRRYSVGSPNVSFVVLETPEDYA
                     NAGIDPPAGYPKEERRQYDALRAEVAGEKQQAAEERLGKVLAIWEEQVAWWNKRFDPR
                     ARRSKRPPEAELSVGVAPPPPPPPPPPPPEGDEAITVTGSRIPSPNLMAAAPVTVTNA
                     DSAGAAGSQGQRPGNQAVIQVEAWAPDRPYLAALDAASPQALEAVLAAQQAKHGELPA
                     FWLDVSEYYHRKGRRAEAVTLLLSALDLATRNSETLAIVAERLMRYNEIDRAIFLYER
                     LLAAEPDRPQPRRALALALARRSRSLAGEPARADLERAISLLTEVIMRPWDEDYEGIE
                     AIALMDVNRLIPRYRALGGGPLALDPRLIRLLDVDMRVLIEWNSDETDVDLWVDEPSG
                     ERAVYNNPRTAIGGRLSNDMTGGYGPEEYLLRRAPTGRFEVRADAYASDALNPNGASR
                     VTARLIRDFGRPTETEEVIDIELLPDDEEDERLIGRIDFGRR"
BASE COUNT         6182 a        13639 c        13275 g         5901 t
ORIGIN      
        1 cagcaccgcc accgccaagg ctgccaagag gacgagcaac ggcacaaagt atgcggcgaa
       61 ggtggcggcc gccccgtcga tgcgcggcgc ggcctcgaca gccggggcgg ccatcgcaag
      121 cggggccggc tcggccggcg ccagcatcgg gagcgcctcg gcgacggcac ccggggccgg
      181 cagctcgatc gaggggagcc acaggctggc gagcgggagc aggatcaggg cgacgaggcc
      241 gagatgcgcg accagcgcct tctccgccgc cgaacggctc cggaacaggc gcagcagtgc
      301 caaagcggcg ccggcgcaca gcaccgactt ccaggcgagc tcgatcagca aatccatcgt
      361 catgccccct tctccaggcc ctgctcgcgg cgcgcctcgg cgatcagccg ctcaagttcg
      421 tcgagctctt ccggctcgat ccgttcctgc atgccgatca gcgccgcggc cgcgccgacc
      481 ggcgatccgt cgaagaaggt cttgatcagg tggcggagcg ccgactgctt gaccttccgc
      541 tgcggcaaag cgggggcgta gagatatttc tggtcgacca agcggcgggt gacgaagccc
      601 ttcttctcga gccgcgacag catgatgcgg agcgcggaat tgctgggcgg gtcggccatg
      661 cgcgcctcaa gctcggcggc gctcgcctcg ccggatgcga acaaagcgtc gaacacctgg
      721 cgttcgcggg gcggcaatgt ctcgatcaag ggtgattctc ctgcaacagt tgttgcatgt
      781 tacatttgtt acatgtgcgc aagccttttg tgatcgctcc cgaaatgtgg gagcgagggc
      841 ggatggcatc ctctttcgct ccgctacgtt cgggctggat gggcgcgatc atcggcactg
      901 cgggatggag catcccggcc agcgaggcgg ggcatttccc ggccgacggg acggcgctgg
      961 aacgctatgc cgcgcgcttt ggaggcgtcg agatcaactc ctccttccat cggccgcacc
     1021 gcgcctcgac ctgggcgcgc tggggggaga gcgtgccggc cgggtttcgc ttcgcggtca
     1081 aggtgccgaa gaccatcacc cacgcacgca agctggtcga ctgcggggag cttgccgcgg
     1141 agttcgttga agaggcgcaa gggcttggcg acaagctcgc gatcctgctc gtgcaactgc
     1201 cgcccaagct cgccttcgat gaggcgctcg cccaacgttt cttcgagcat ttatcgggcc
     1261 tgacccccgc aacgatcgtc tgcgagccgc ggcatccgag ctggctcgaa gcggcggccg
     1321 acgccctgct cgacagcctc ggggtcgcca gggtcgccgc cgatcccgcc atccttcccg
     1381 ccgccaatgt ccccggcggc tggcgcgggc tcagctattg gcggctgcac gggtcgccgg
     1441 ccatgtaccg gtcggcttat gacgacgcgc ggctcgacga ttatgcccgt cagatcgggg
     1501 cggagccggg gcaggcctgg tgcatgttcg acaataccgc cgcctcggct gcgaccggcg
     1561 acgcgctcaa gctgatggcg cggctgtgac cgagcgggag gacaggcacg ggccctcggc
     1621 ggccgtcgcc tggctggtcc acggcttcac ggcgatgggg gcggtgctgg ccttcctcgc
     1681 gctgatggcg atcctggagg aagattgggc cctggcgctc ggctggctgc tggcggcgct
     1741 ggtcgttgac ggggtcgacg gatcgctggc gcgctgggcg aaggtcaagg cgcgggcgcc
     1801 gcggatcgac ggcgaaacgc tcgacctcgt cgtcgactat ctcaattatg tgttcgtgcc
     1861 ggcggtgctg gtctggcggg cggggctggt gccggagcgc gccgcaccct ggctgtgcgg
     1921 gctgatcctg gtctcggcgc tgtacaattt cacccggcgc gacctcaaga ccgaggacaa
     1981 ttacttccgc ggcttcccgg cgctctggaa cgtcgtcgcc ttctacctct tcgtggcgcg
     2041 gccggggccc gaagccggcg cggcggcggt catcctgttc gccgccctca ccttcgcgcc
     2101 cgtgcatttc gtccacccgt tccgggtcag cgattatggc cgctggctgc cggcgctggc
     2161 gaccgcctgg gcggcggcga cggcggcttt gctgtggccg ggctggaacg aggaggcgcg
     2221 gtccgcctgg ctggccgtct cgctcggcgc ggcggcgatc ctgctggcgc tcggcctcgc
     2281 ccgcaccgtc cgggggcagc ggccggagcg gctcagacga tgaagtcggt cgccgccagc
     2341 gcctgctcgc cggtgaggca cagcatgata tcggcggcgc cgtcgccgtc gcggtcgcct
     2401 tcgacatagg tgtagccgcc gtcggcaaag gcgcggagct caccggcgac gccgctgaag
     2461 tcggcgtccc cgatgaagtc gaacgcctga tcgcccgccc cgctgaagct cgcgtcgatc
     2521 cggtgcaggt cgatccgatc cccctcggcg ccggcaaagt cggcgatgca gtcggcggtc
     2581 agggcggaac cgccggtttc gccttcgcgg aacacgaagc cgtcggcgcc ctcgccgccg
     2641 cgcagcgtgt cgcgatcgaa gccgccatag agtaggtcgt cgccgccgcc gccgatcagc
     2701 aggtcccgcc cgtcatcgcc gaacaggcgg tccgcttccg atccgccgtc gatgcggtcc
     2761 gcctccgagc cgccgctgag gcggtcctcg ccgccgtcgc cggacacgtc gtcgcgcccg
     2821 gcgccggcgt cgatcgtgtc gcgtccatcg cctccggcga gccggtcgtc tcccgagcgg
     2881 ccgaagatga agtcgtcgcc gccctccccg gcaatcgcat tgtcgaggtc gctgccgttg
     2941 atgaggttgg ccagaccgtt gccggtgcct gcccgcgccg cgccggtgag gcgcagctgc
     3001 tcgacctcgg cggccaggat atgatcgacg ccggccagca cgacgtcgtc gcctccgccg
     3061 acctgctcga tgacgcggtc ggcggcatcg tcgacgacat agatatcgtc gccggtgccg
     3121 ccgtccatgg cgtcgccgcc gccgccgccg tccatccgat ccgcgccgtc gcccccgaac
     3181 agccgatcgt cgccggtgcc gccatcgatg aggtcgtcgc tattctggcc cgacacgaaa
     3241 tcctggccgt caccgccgct caagccgtca tcgtggttgc cgccgctgag cgcatcgtcg
     3301 ccgccgcctc cggccagcac gtcattcccg tcgcggccgc ggagccggtc gttgccgtcg
     3361 ccgccggcga tgcgattggc gatcaaatcg cccgacagca agtcgctgaa cgccgacccg
     3421 acgagatgct cgatgccgct caggacatcc cggccgtcgc ccgtgatttg cgcttcggtg
     3481 acaagaaggt cgaccgcggc cgcggccgcc gccccggccc aggagccggt atcttcaccc
     3541 tcgccgccgg cgagccggtc attgcccgca ccgccttcga gcaggtcggc gccggcgccg
     3601 gcgtcgagcc ggtcgttgcc ggccccgccc gaaagctcgt cattcccttc cgcgccgcgt
     3661 agcgtatcgt tgccgccgag gccgctgatc ccgttatcgc cgccattgcc gaggatcagg
     3721 ttggacccgg aattgccggt gccgtttgcg tcgcccgcgc cggccagcac gagagtctcg
     3781 agcccctcct gaagcgcata gctgccggcg acgatcaccc ggtcgcggca tcccgcatcg
     3841 acgatgacgt cgatgaggtc gacatgatag acatcgtcgc cctcgccgcc gtccagccga
     3901 tcagccgcct cgccgaggtc agccccgaac agcgtatcgt tgccgccgag gccgatcagc
     3961 tcatcggcgc cgcggccgcc gtccagccgg ttgcggccgc tcgtgccggt gatgatgttg
     4021 tccccgtcat tgccatagcc gaaataggcg ctgccggtga gctcgagcgc ttcgacatgg
     4081 gcggacaatc ggtgcgacgc ggcggcgacg atcgtatcga aaccttcgcc gccaccttcg
     4141 atcaccctgg cgagacttcc ggcgccgagc tcgtaacgat cgtcgccgga gccgccgaac
     4201 aaacggtcct ctccttcaag gctgataagg aggtcggcgc cggctctccc cagcagcagg
     4261 tcgtgctccg cctggccatc gagccggtcg tcgccgccgc ccccggaaag ccggtcatag
     4321 ccgccccccc cgtagagccg gttgtcgaga ctgttgccgg tgatgacatt atggccatcg
     4381 ttgccgccgc cctcgatcgc gccgccggtg agatcgagat tctcgacctc gtccgtgagg
     4441 gtggtgacat cggtgctcgc gaccagggtg tcggtgcctt ggccggccag ttcgacgatg
     4501 gagccgccgg cgccctggat gtagcggtcg tcgccggcgc cgcccgcgag ccggtcgacc
     4561 ccgccgccgc cgtcgagcaa gtcgttgccg ccaccgccgt agacacggtc gtcgccgtcg
     4621 cccccgtcga gcgcgtcgtt gccgtcaccg ccgtcgatat ggtcgtcgcc accgccgccg
     4681 ccgagcaagt cgtcgccgtc acctccgttg atacggtcgc ccccaccgcc gccgtcgagc
     4741 aagtcgtcgc ccgccccgcc accgatgtgg tcgtccccgc cgccgccgga cagccggtcg
     4801 ttgccggcca gcccgacgag ataatcggcg agttcggtgc cggcggcgac gtcctccccg
     4861 gcagagcctc ggaaaacgct ggcaaagtcg tgggcggtga gccgctgcag atcgaccatg
     4921 aacgaacccg ccccggtggg cgatctgcct gcaacgatcg caaaaagatc gccgtcggcg
     4981 agacgaccca gagcgatgtc cggccgaaac cgggcattgg gctccccaat ggcgatctgc
     5041 tccccgatca gggttccgtc cgatgccaga gcctgtcccg tcgcagtgcg cgactcgctg
     5101 tcgtcaccgg ccgccccggc gacgaacaag ctgccatcag gaccggcgga aagttcggcc
     5161 gggacgcccc tcgactcggc gctgctcacg tcgaattcgg gataggcccg cccctgatcg
     5221 atgacacgcc cgcgcaccct cgagctctcg tacggcggct ccggctcgcc cgccccgaac
     5281 gactcgtaga agctttcggt ccagaccagc gcgtagcccg tcggcgtggc agcgagggaa
     5341 ggaccgaaac agttgagcca cttcccggcc cggtcgttgc catagctgtc ggatgtttcc
     5401 ggattgccat ggaatatcga tgcgtccccg gcctcaccga aaccatcgaa gcggcgcgtg
     5461 gtgatccggg tcagctcacc atccacaaaa tcgtcccatt cgatgtcgct cgaataagcg
     5521 actacaaacg cgccatcggc gagcgcgacc acgtccagcg agccataaac ctcggcaacc
     5581 aacagctccg ccgcgccgag cgcgagacct tccgcggaga agagctgccc catcagcctc
     5641 tcacctggac cggcatcgtt ttcaccccaa atggcgagca cgttgccatc ggaaagggtc
     5701 gtcagctggg cataggggcc ggacagcttg acgctcggcg aaacaggcgc cccgttgaaa
     5761 ttgaaggcgc ggagctggcc agcgtagggg acaccataag agagggtgaa gccggcggaa
     5821 gtggcgacca agtcatcgac aacgctgtaa ccctccacca aatttacggg ttcaccggcc
     5881 ggcgcgcctt gcgcgtccag aaattgccca cgaatgacag gcgcgccggt cgcttcgacc
     5941 tggtcggccc agacgatgat ttgccgcccg tcggcaagtt cgacggcgtc aaccgatgca
     6001 ttggcggtat taccatcaat ggcaacgccg ggagtttccc tgaaataggc tacggtcatg
     6061 ctcgccccca catcggccaa cccgcgccgc ctcggacccg cctagcacag cggaccgctg
     6121 gaggaacaag tcgttcccgg cattgagttt tgaggttccg gcaagggact gcggcggcgg
     6181 ttcagatggc gctgcctgca caaggtctgc acgccaaacc cttcccttct cccgcgcggc
     6241 ggccgcagcc tctccatccc caaagtgcat cgagcgaacc gcacagtcag cgggagctcg
     6301 cccatgtcgc ctttcaccag ccgcgcaggc tattccttct ggatcaaggc ggcgatggcg
     6361 ctgctgctgg tggtgctcgg cgacctgctc ttcttccggc aatggctcgg cgcgacgctc
     6421 ggcctgttcg cgatcgcctg gctggccggc ctgctggccg cggctccggc cgccatgcgc
     6481 aagcagccgt cggcgctggc tgccctggcc gcgctcgctt tcgccctggt catgatcgag
     6541 gatccgagcc cgcttgcctg gggggccttc tggtgcgcgc tcagcctggc tgccttgctg
     6601 ccgcggatgg cgcggttcgg cgccgcctcg cagatggcgg tgcggctgtt cctgcacggc
     6661 ctttccgcga tcatcgcgcc gtggagcgac ctcaacaggc tgcggcggct gcggccctct
     6721 gaaaggcgcc cgcgcatccg gtcgctcttg ccgctgctcc cgctgccact cgcgggagga
     6781 gcgctgttcg tcgggttgtt cagcatcgcc aatccactga tcgccaacct gttcacggca
     6841 gtcgagctgc ccccaccgaa cctcgaccgg gtgcgcatcg ccttgtggat cgcgcttgcc
     6901 ggcatggtct gggcaagctt gcggccgcgg gcgggcgtca cccgaccgtg ggacgctcat
     6961 gtcatcgacc gggggccgct gcccggcgtg tcggtggcgt cggtgacgct gtcgctgctg
     7021 ctgttcaacc tcatcttcgc ggtccagaat gggctcgaca tcgctttcct gtggagcggc
     7081 gctcggcttc ccggtgcgat gacgctggcc ggttatgccc atcgcggcgc ctatccgctg
     7141 atcgcgaccg ccctgctcgc cgggctgttc gtgctggtga cgctgcggcc gggctccgag
     7201 acggcggcgc ggccgctgat ccggcggctg gtggtgctgt ggatcggcca gaacatcttc
     7261 ctggtggcgt cgagcgtgct gaggacgctc gattatatcg acgcttattc gctcacccgg
     7321 ctaaggatcg cggcgctggc gtggatgctg ctggtagcgc tcggcctcgc cctcatctgc
     7381 tggcggatgc tgaaggagcg gagcggcacc tggctgatca acaccaacgc ggccgccgcg
     7441 gccgcgatgc tgagccttgc caccatcgtc gacctcggcg cggtcgcggc gcattggaac
     7501 gtgcgccatg cgcgtgaagt cggcgggcgc ggcacgcata tcgacctctg ctatctgaac
     7561 cagctcggcg catcggcggt gctgccgctg atcgagctcg aagcgcggcc gctgccgccg
     7621 gccctgcgcg accgcgttgc ctgggtccgg atcggcgcga tggacgggct cgagacccgg
     7681 caggcggact ggcacagctg gacctggcgc aacgcgcgcc ggctggcggc ggcgcgggcg
     7741 gcggagccgg cgccggtgac gctgccgcag gcgcctttcg gtagaaaatg cgacggcagc
     7801 gtctggacgc cgctgccggt cccttacccc gccgggacgc gctcgccctc ccccgccctg
     7861 ccctcccccg cccccgcagc gccggcggag ccgcggttga cagcggagca gggccgatga
     7921 ttgagtgccc catgccgcgc acgatccttg tcgccgacga cgatccccac atccgccagc
     7981 tgctcgtctt cgcactcgcc aaggcggggc tcgccgcgat cgaggccgag gacggcgagg
     8041 cggcgctggc ggcggcgcgc gagcaaagcc cggatctcgt catactcgac atcaacatgc
     8101 cgcgcatgga cgggctcgaa gtgtgccggc ggctgcgcgc ggacggcggc atgccggtgc
     8161 tgttcctgtc ctcgcgcgac gacgagatcg accgggtgct gggcatcgag ctcggcgccg
     8221 acgattatgt caccaagccg ttcagcccgc gcgaagtggt ggcgcgggtg caggcgatcc
     8281 tgcgccgcat cgccgcccac ccgccggcgg tggagggcgc cttcccggcg atccggcgcg
     8341 gcctgctgac gctcgacatc gacggctggc aggcgcgctg gggcgaggcc gaggtgccgc
     8401 tgacggtgac tgagttcggc atcctcagaa cgctcgcggg gatgccgtcc aaggtcttca
     8461 cccgcgacgc gatcatcgac cggcttcgcg ggcccggctt cgccctcacc gagcgcacca
     8521 tcgacagcca tgtccgcaac ctgcgcgcca aattcgccca agccggcggc ggcgacgtga
     8581 tcgataccag ggccggggtg ggctaccggc tcggcccctg cggcggcggg agcggatgac
     8641 cggcggcgtc aaggcgctca tcaagcgata ttggccccgg cttcggctcc ggaccatcct
     8701 gctcgccacc ttgctgttcg tggccgcttt gcccggcgtc ggcgcgctgt tcctgcgggt
     8761 ctatgagaat acgctcgtcc gccagaccga atccgagctg atcgcgcaag gcgccgcgct
     8821 ggcggcggcg gcggaagcgg cgtggcccgg gagcgcgctg ccggcggcgc ggcggctgag
     8881 gcccgacgaa tatcgccccg agccgcccca gatcgacctc aacggcaccc cgatcctgcc
     8941 cgagcggccg gcgccggcgc gggggtcggg gctggatccg gccgcggtcg cggcggcggg
     9001 gcggatcgcg ccggtgatga tgcgtaccgc gcagaccacg ctcgcctcga tccagctgct
     9061 cgaccggcgc ggcaccgtcg tcaccgggcc gctggccggc gccagctacg cgcggctccc
     9121 ggaagtcgcg ggcgcgctgg cggggcggcc gaccaccgtc cttcggcaca atggcagcta
     9181 ccgccccgaa tatccgctcg aatggctgag ccgggcatcc gaccttcgca tccaccatgt
     9241 ccggccggtc agcgccggcg gccaggtcgt gggcgcgctg ctgctatcgc gctcgccgcg
     9301 ggcgcttttc cgcgggctct acgaagatcg cggcaagatc ctgttcggaa tcgccgccat
     9361 cttcgccact ttggtggtgc tgtcggggct gctgtcgcgc ggcatatcgc ggccgatcga
     9421 gcggctcagc gcggcgacgc ggcggctggc gctcggcggc ggcggcgaag tgccggagcc
     9481 gccgcagacc gccgcggtcg agatccaggc gctgtaccgc gatttcgccg acatggccga
     9541 agcgatcgag cgccgctcgc gctacctgcg cgatttcgcc catgccgtca gccacgagtt
     9601 caagacgccg ctcgcgggca tcaggggcgg gatcgaattg ctccaggacc atcaggcgag
     9661 catggcggag gccgagcgcg agcgcttcct cgccaacgtc gcggccgacg ccgagcggct
     9721 ggcgcaattg gtcagccgcc tgctccagct ggcgcgggcc gacatggcgc gacccgatcc
     9781 cgacgccgcc accgcccccg cggcgctacg gagtctggtg gacgcggccg gggggcgtgg
     9841 catcgcgctg cgcggcgacc tgccggagga cttgccggcg ctggcggtgc cgccggcgac
     9901 ggtcgaggcg gcgctggcga cccttgccga caacagcctg caggccggcg cgacggcgat
     9961 cgagatcgtc gcgcgggcgg aggcggacgc gctggtgatg gagataggcg acaacggccc
    10021 cggcgtcccg ccctccgacc gcgagcgcat cttcgaaccc ttcttcacca gccgccgcag
    10081 cgccggcggg accgggctcg gcctccccat cgtccgctcg ctgctcgaag cgagccgcgc
    10141 gaccatcgag ctggtgcctt cggccgccgg cgcccgcttc aggatccggc tgccgctggc
    10201 gtgacgcggg cggtccccgc catcaccgct cgtaaggtat gatcgagatg ccgccgccgg
    10261 cttccaagat ggcgtgcctc acctgcccga cccgctcgag cccgtggttc gcgcgcgccg
    10321 cgaccatgat ctcgtcgagc ccgatccggg cttcactcag gcggtcgcgc atcacctggc
    10381 cgtcctcgat cagcagcacg ggccgaccgg agacgatcgt ctggaagcgc ggccagcgct
    10441 ccttgaggac cgacatggcg atgccggccg cgaccaaagt gatgatggcg agcccggcat
    10501 tggtcatcga agggtcgctg tcgatcatcg cttcctgcgt ggtctcgctg atgatcagca
    10561 ggatgacgag ctcgaagctc gacatctccg ccagcgaacg ccggccggcg atgcggaaca
    10621 cgaggagcag gaagaaatag gtgcaaaggg cgcggatgac ggattccaag ggggccctct
    10681 cctcaggggt agacgaactg gttgaagcgg acctctgggc cgccctcgat cccgatccgg
    10741 gcggggatgt cggcgaacgg gccgtcggcc tgataatggt agaggatgac ggtttcgcgg
    10801 ccgccgagcc gcggcgcctt caagaggtag gtatgcccgc cgggccgatc tccacggcct
    10861 ccggctcggg cgcgacattg tcgagctggg tggcctcgag gaaggcgcgg ttgacgtgga
    10921 ggcggatgac gcccgccccg ggcggcggca ccacccggat gcgcaggtcg gccggggcgt
    10981 ggttgcgaac gaaccgctca tattcgacgc tgagggcggg gccggcccgg gcggtgcggc
    11041 tgctcagcgg ccccggtccg agcaacccgg ccagcgccgc cagcaccagc agggcgagca
    11101 gcagccagcc gacgcggtga aaccgctcct cgcgaggcgt gacgccggag ccctcgggga
    11161 tttcgaggcc ggcgtcttca gtcttctcgg cagcggccat ggacggcgct cacgacggtt
    11221 tccgccgggg attacgttat aaaggccatg tcggttccgg atcgctagcc gcgcgcaagc
    11281 tggatgcgga tggtctggac cccgtcggcc acggcgacct caaggatccg gtcctcgccg
    11341 gccgcttcga tgatgctgac tcgcccgctg cgctcgatcc gggcctcggc gacgcgcgag
    11401 gcgtcggcgt ggccgtggcc gcgcagttcc tcgtcgaggt cgccttgggt catgtggacc
    11461 ttgcgcaagg cggcttcatc gggcgttccg gcgcggacca gcagcctggc atcgcccttg
    11521 gccagccggc tgaaaccgcg ccagtgcagc gcgagcccgg agaccagcca gtgcatcgcg
    11581 acgatcgcgg ccgcggcggc cagcgtcggc ccgagcggcg cgttgccggt gagaccgcgg
    11641 ctgacggtcg agccgaggat gatgccgacg atcacgtcga aggcactgtt gctgcccatg
    11701 aagcgcttct tgccgaggcg gacgatggcg agcgtgacgg cgtaggtcac cgccgcccgg
    11761 agcgccatct ggccgatggt gaggtccatc gactgccggt ccaggccgag cgcggtttcg
    11821 aagatttcca tcgagccgtt cctctactat tgtggcgggg gagcgcagga agccgcatgc
    11881 ggcgccggtg aaaagcggga ggccggcatc atggttccgg gcgcctggaa ccgaggtccg
    11941 ggcttgacct tcgcgctccg cactcctaga tagcggccct caccacagcc cttcggaaca
    12001 ggtctcgccg tcgcgcagcg gggccaacgc attgattggc tgaggtttca taggacgctg
    12061 aagctgcggt ttccaggtgg agactcgcgg ctttttgcgt ctttcgcgtc cgcgaagaag
    12121 atggctgggc tccgctttca gggtggaacg cagcaaagac gaggcaaaaa aacatctatg
    12181 gcgaccatcg ctccccaggc cggcgaactc cgctcgaccg cgtccaagcg catccgcaag
    12241 atcttcggca acatccacga agtctccgaa atgccgaacc tcatcgaggt tcagcgcgag
    12301 agctacgagc agttcctccg ctcgcggccc caggacggct atgtgtcggg cctcgaaaag
    12361 acgctccggt ccgtgttccc gatccgcgat ttcgccggca cggccgagct cgacttcgtc
    12421 aattacgagc ttgaggatcc caagtacgac acggacgagt gccgccagcg gggcatgacc
    12481 tatgccgcgc cgatgcgcgt gacgctgcgc ctgatcgtgt tcgaggtgga ccccgatacc
    12541 gagacccgct ccgtgctcga tatcaaggag caggacgtct acatgggcga catgccgctg
    12601 atgacgaaga acggcacgtt catcgtcaac ggcaccgagc gcgtcatcgt cagccagatg
    12661 caccgctcgc cgggcgtgct gttcgaccat gaccgcggca agacccacgc gtccggcaaa
    12721 tatctcttcg ccgcgcgcgt catcccctat cgcggctcct ggctcgactt tgagttcgat
    12781 gccaaggaca tcgtcaacgt ccgtatcgac cgcaagcgca agctgccggt gacggccctc
    12841 ctgcacgcgc tcgacatgag ctcggaggag atcctcaaca ccttctacaa caccgtgacg
    12901 ttcgtgcgcg cgccgaacgg ctggcagatc ccgttcgtgg ccgagcaatg gcggggccag
    12961 aagccgagct tcgacctcgc caatggcgag accggcgaag tggtgttccc ggccgggcag
    13021 aagatcaccc cgcgcggcgc caacaaggcc gccaaggacg ggctcagccg gctcctcatt
    13081 ccgaccgagg agattttcgg ccgctattcg gcgttcgacc tggtcaacga ttcgaccggc
    13141 gaaatctata tcgaggccgg cgatgaggtt tcgcctgaga atctggagag gctcgaccgc
    13201 gccggcatcg accggatcga gctgctcgac atcgatcatg tcaacaccgg cgcctggatg
    13261 cgcaacacgc tcaaggccga caaggccgag gaccgcgacc aggcgctcag cgacatttac
    13321 cgcgtcatgc gccccggcga gccgccgacc cgcgagacgg ctgacgccct cttcgccggc
    13381 ctgttcttcg atcccgagcg ctacgacctc tcggccgtcg gccgcgtcaa attgaacatg
    13441 cgcctcgacc tcgacgtcga ggacacggtg acgacgctcc gcaaggagga catcatcgcg
    13501 gtcgtaaaga ccctcgtcga cctcaaggac ggcaagggcg agatcgacga catcgacaat
    13561 ctcggcaacc ggcgcgtccg ctcggtcggc gagctgctcg agaaccagta tcgcgttggc
    13621 ctgctccgca tggagcgcgc cgtgaaggag cgcatgtcct cggtggacgt gtccaccgtg
    13681 atgcccaacg acctcatcaa cgccaagccg gcggtggccg cggtgcgcga attcttcggc
    13741 tcctcccagc tgtcgcagtt catggaccag accaacccgc tcagcgaagt gacgcacaag
    13801 cgccgcgttt cggccttggg ccccggcggt ctcacccgtg agcgcgcagg cttcgaagtc
    13861 cgcgacgtcc acccgaccca ctatggccgc atctgcccga tcgagactcc ggaagggccg
    13921 aacatcggcc tgatcaacag cctcgcctcg ttcagccggg tcaacaaata cggcttcatc
    13981 gagaccccgt accgcaaagt cgtcgaccat aaggtgacgg acgacgtcgt ctatctgtcg
    14041 gcgatggaag aggccaagca cacgatcgcg caggccaatg ccgagctttc agacgacaag
    14101 catttcgtcg aggagatcgt ctccagccgc caggccggcg agttcctgat ggcgccgcgc
    14161 gacatcatca ccctgatgga cgtgtcgccg aagcagctcg tgtccgtggc cgcctcgctc
    14221 attccgttcc tcgagaatga cgacgccaac cgcgcgctga tgggatcgaa catgcagcgc
    14281 caggcggtgc ctttggtccg cgccgaggcg ccgttcgtcg gcaccggcat ggaagagacg
    14341 gtcgcccgcg attccggcgc cgccatcgcg gcgcgccggt cgggcatcgt cgaccaggtc
    14401 gacgcgaccc gcatcgtcgt tcgcgccacc ggtgaggtga atgcgggaac gtcgggcgtc
    14461 gacatctata ccctcatgaa gttccagcgt tcgaaccaga atacctgcat caaccagcgt
    14521 ccgctggtga aggtgggcga cgtggtgaac gccggcgacg tcatcgccga cggcccctcc
    14581 accgagttcg gcgagctcgc gctcggccgc aacgtgctgg tcgcgttcat gccgtggaac
    14641 ggctacaatt atgaggactc catcctgatc tccgagcgga tcgtgaagga cgacgtcttc
    14701 acctcgatcc acatcgagga gttcgaggtg atggcccgcg acaccaagct cgggcccgag
    14761 gacatcaccc gcgacatccc gaacgtcggc gaggaagcgc ttcgcaacct cgacgaggcc
    14821 gggatcgtct atatcggcgc cgaggtcgag ccgggcgaca tcctgtgcgg caagatcacg
    14881 ccgaagggcg aaagcccgat gacgccggag gagaagctcc tccgcgccat cttcggcgag
    14941 aaggcctcgg acgttcgtga cacctcgctg cggctgcccc cgggcgtcgc cggcacggtc
    15001 gtggaagtgc gggtgttcaa ccgtcacggc atcgacatcg acgaccgtac ccgcgccatc
    15061 cagaccgagg agaaggaccg gctccgcaag gatgcggacg acgagcgttc gatcctgaag
    15121 cgcgccacct actcgcggct tcgcgagatg ctgctcggcc agaccgccac ggcggcccct
    15181 aagggcgtga agaagggcgt ggcgatcgac gaggcgacgc tggagagcgt ggagcgccac
    15241 gaatggtgga agttcgcggt caaggacgat gccgtccagg cgaacctcga agccgtcaag
    15301 ggccagtatg acgaggccga ggcggtgatc cgccgccggc tcgaggaccg catcgagaag
    15361 ctcgagcgcg gcgacgagct gcccccgggg gtcttgaaga tggtcaaggt gttcgtcgcg
    15421 gtgaagcgca agctgcagcc gggcgacaag atggccggcc gccacggcaa caagggcgtg
    15481 atcagccgca tcctgccgat cgaggacatg ccgttcctcg aagacgggac gcatgcggac
    15541 atcgtcttga acccgctggg cgtgccgagc cgcatgaacg tcggacagat tttcgaaacc
    15601 catctcggct gggccgcgcg cggcctcggc aagcagatcg gccagatgct cgaagacatt
    15661 cacggcaagg gcaaggcgct ggaaggcgcc gacgtcacca acgtgcgcaa caagctcaag
    15721 cagatctatg gcgagaatta cttcgccgat ctcgacgcgc ggaccgacga ggatctgatg
    15781 gagctcgcct ccaacctggt cgcgggcgtt ccgatgggca ctccggtgtt cgacggcgcg
    15841 cgcgaagcga acgtgtcgga gatgctgacc atggcgggcc tcgatccgtc cgggcaggtg
    15901 acgctctatg acgggcgcac cggcgaggcg ttcgaccgca aagtgacggt gggctacatc
    15961 tacatgctga agctccacca cctcgtcgac gacaagatcc acgcgcgttc gatcggcccg
    16021 tacagcctcg tcacccagca gccgctgggc ggcaaggccc agttcggcgg gcagcggttc
    16081 ggcgagatgg aggtctgggc gctccaggcc tacggcgccg cctacacgct gcaggagatg
    16141 ctgacggtga agtcggacga cgtgatcggc cgcaccaagg tctacgaggc gatcgtcaag
    16201 ggcgacgaca cgttcgaggc cggcattccg gagagcttca acgtgctcgt caaggagatg
    16261 cgctcgctgg gcctgaacgt cgagctgaac tcggtgatcg atccggatgc ggacgaccag
    16321 cagcagctgg ccgcggagta accgcctgag ccctctcccg cgtgcgggag aggttgggtg
    16381 agggtcttct tcctttgtga acagaccctc acctcccaag cctggcggct tgggtccctc
    16441 ctctcccgca agcgggagag ggtaagaatt ctagagaggg tttgaaccca tgaatgaact
    16501 gagcaacttc gccaatccgg tcgtgaagcc ggagacgttc gaccagatcc agatcggcat
    16561 cgcctcgccg gagcggatcc gcagctggtc gttcggcgag atcaagaagc ccgagaccat
    16621 caactatcgc acgttcaagc ccgagcgcga cggcctgttc tgcgcgcgca ttttcggtcc
    16681 gatcaaggac tatgagtgcc tgtgcggcaa gtataagcgc atgaagtata agggcatcgt
    16741 ctgcgagaag tgcggcgtcg aggtgaccgt ctccaaggtc cgccgcgagc gcatgggcca
    16801 tatcgagctg gccgctccgg tcgcccatat ctggttcctg aagtcgctcc caagccgcat
    16861 cggcctgctg ctcgacatgc agctgaagca gctcgagcgc gtgctctatt tcgaagccta
    16921 tatcgtcatc gagccgggcc tgaccccgct cgagaagtac cagctcctca ccgaggacga
    16981 gctccttgag gcgcaggacg aatatggcga ggacgccttc tccgccggca tcggcgccga
    17041 ggcggtgcgc gtcatgctcg agaatctcga cctcgaaggc gagcgcgaag agcttttgaa
    17101 ggagctggcc gagaccaagt cggagctgaa gcccaagaag atcatcaagc gcctgaaggt
    17161 cgtcgagagc ttcctcgaat cgggcaaccg gcccgagtgg atgatcctgg aagtcattcc
    17221 cgtgatcccg cccgagctgc gcccgctggt gccgctcgac ggcggccgct tcgccacctc
    17281 cgacctcaac gacctctatc gccgcgtcat caaccgcaac aaccggttga agcgcctgat
    17341 ggagctgcgc gcgccggaca tcatcgtccg caacgaaaag cgcatgctgc aggagtccgt
    17401 ggacgctctg ttcgacaacg gccgccgcgg ccgcaccatc accggcgcca acaagcgtcc
    17461 gctgaagtcg ctgtccgaca tgctcaaggg caagcagggc cgcttccgcc agaatttgct
    17521 cggcaagcgc gtcgactatt cgggccgctc ggtcatcgtc accggtccgg agctcaagct
    17581 ccaccagtgc ggcctgccca agaagatggc gctcgagctg ttcaagccgt tcatctactc
    17641 gcgcctcgac gccaagggtc tgtccatgac cctcaagcaa gcgaagaagt gggtcgaaaa
    17701 ggagcgcaag gaagtctggg acatcctcga cgaggtgatc cgggagcatc cggtgctgct
    17761 gaaccgcgct ccgacgctcc accgtctcgg catccaggcg ttcgagccgg tgctgatcga
    17821 gggcaaggcg atccagcttc acccgctggt ctgcgcggcg ttcaacgccg acttcgacgg
    17881 cgaccaaatg gccgttcacg ttccgctgag cctcgaggcc cagctggaag cgcgcgtcct
    17941 gatgatgtcg accaacaaca tcctctcgcc cgccaacggc aagccgatca tcgtgccctc
    18001 gcaggacatg gtgctcgggc tctattatct gtcgatggag cgtgagggcg agcccggcga
    18061 gaacatgatg atttcggaca tgtccgaggt ccaccaggcg ctcaacgcca aggccgtgac
    18121 gctccattcg aagatcgtca gccgcgtccc gcagacggac gagcagggca agacctacat
    18181 gaagcgcttc gagacgacgc cgggccgcat gctgctcggc gagacgctcc cgaagagcca
    18241 caaggtgccg ttcgagaccg tcaaccgcct gctgaccaag aaggagatcg gggacgtcat
    18301 cgacgtcgtc tatcgccaca ccggccagaa ggagacggtg ctgttcgccg acgccatcat
    18361 gggcctgggc ttccgccatg cgttcaaggc cggcatctcg ttcggcaagg acgacatgat
    18421 catcccgcac gaaaaggtgg gcatggtcga cgagacccgc gcgctcgtga aggattacga
    18481 gcagcaatat caggacggcc tgatcacgca gcaggagaag tataacaagg tgatcgacgc
    18541 ctggagccgc tgcggcgacc aggtggccaa cgccatgatg gaaaagatca aggcgcagcc
    18601 gaaggacgag aacggccgca tggccccgat caactcgatc tacatgatgg cccattccgg
    18661 cgcccgcggt tcgcaggccc agatgaagca gctcgccggc atgcgcggcc tgatggccaa
    18721 gccgtcgggc gagatcatcg agacgccgat catctcgaac ttcaaggaag gcctcaccgt
    18781 ccttgagtat ttcaactcca cccatggtgc tcgtaagggt cttgcggaca cggcgttgaa
    18841 gaccgccaac tcgggctacc tcacccgccg cctcgtcgac gtgtcgcagg attgcgtgat
    18901 cgtcgaggag gattgcaaga ccgagaacgt gctcgacatg cgcgccatcg tccagggcgg
    18961 cgccgtcatc gcgtcgctcg gcgagcgtat cctcggccgc acgccgggcg aggacatcgt
    19021 cgacgacaag accggaacgg tgctcgcgaa ggaaggcgaa ctgatcgacg aggccgcggc
    19081 cacgcggatc gacgagatgg gcatccagtc ggtgaacatc cgttcgccgc tggtttgcga
    19141 gagcgagcat ggcgtgtgcg gcaaatgcta cgggcgcgac cttgcccgcg gcacgccggt
    19201 caatatcggc gaggctgtcg gcgtcatcgc cgcgcagtcg atcggcgagc cgggcaccca
    19261 gctgacgatg aggaccttcc acatcggcgg cgcggcccag ctcaacgaga cgtcgagcct
    19321 ggaagcctcc accgacggga ccatcgagct tcgcgacctt ccgacgatcc tggacgcccg
    19381 cgggcgccgg gtggcgatgg cgcggaacgg cgagctcgcc atcctcgaca tggacggccg
    19441 cgagcgggcg gtgcaccgta tcccctacgg tgcccacctg atggcggatc acggccacat
    19501 cgtctcgcgc ggcgaccgta tcgccgaatg ggatccgttc atgatgccgg tcatcaccga
    19561 gaagggcggc gtcgtgaagt tccaggacat ggtcgagggc aagacgctca ccgagcaggt
    19621 cgacgaagcg acgggcatcg cccagcgcat cgtcaccgag ttccgcggct ccgcgcgttc
    19681 gaaggaggat ctgcgcccgc gcatcaccct cctcgacgag gagagcggcg aggcggcgcg
    19741 ctacatgctg tcgcccggcg cgatgctgtc ggtcgaggac ggccagcaga tcggcgccgg
    19801 cgaagtgctc gcccgcgtct cccgcgaagc cgccaagacc cgcgacatca ccggcggcct
    19861 gccgcgggtg gcggagctgt tcgaggcgcg caagcccaag gagaatgcga tcatcgcgaa
    19921 ggtctcgggc cgggtcgagt tcggcaagga ctataaggcc aagcgcaaga tcggcatccg
    19981 tcccgaggat ggcggcgagc tggtcgaata tctggtgccg aagtcgaagg tcatcgacgt
    20041 gcaggaaggc gacttcgtga agcgcggcga caatctcatc ggcggctcgc ccgatccgca
    20101 cgacatcctc gaagtgctcg gcgtcaaggc gctggccgag tatctcgtgt cggagatcca
    20161 ggaagtgtat cgtctgcagg gcgtgaagat caacgacaag cacatcgagg tgatcgttcg
    20221 ccagatgctg cagaaggtcg agatcaccga gagcggcgac accaccttcc tggtcggcga
    20281 acaagtcgat caccaggaga tgcgggaggc gaatgccagg ctgaccaaca agcagcgcaa
    20341 ggccgaaggc aagccggtgc tgctcggcat caccaaggcg agcttgcaga cccgcagctt
    20401 catctcggcc gcctccttcc aggagacgac gagggtgctc accgaagcgt cggtgcaggg
    20461 caagatcgac acgcttgatg gcctcaagga gaatgtcatc gtcggccgcc tgatcccggc
    20521 gggtaccggc gccggcatga accggatgcg gatcgcggcc accagccgcg acgccgcgct
    20581 ccgcgcccag catcgcaagg tgcaggcttc gctgatcgcc gccaattcgg cggaggaaga
    20641 gcatgcggcc gagctcgccc aggggcccga ggcggcgatc ggcgacgatc cgctcggcga
    20701 ggtggcctcg gtcggacacg gcaccgatgc cgatgccggc gagtatctgc agacctgagc
    20761 ctgctcagga ttgtgacctg atcaggatcg tgactgggaa gcgccccgcc ggaccctccg
    20821 gcggggcgtt ttcgttggag gcggcatgaa gacgctgatc accttgctgg cgctgatcct
    20881 gctggtcgtg ccgatgctgg ccgggatccg gcggatgcgg cggctgcccc gcaagccgga
    20941 cgaggattga gcgccggcga gaagcccctc gtcggacaag cgattggcct ttgcccgggc
    21001 gagcggctat cggggcggcg atggagacgt ccgaccttcg catcgccatg ttcagcggca
    21061 attacaatta tgtccgcgac ggcgcgaacc aagcgctgaa ccggctggtc gactatctcg
    21121 ccaagcgcgg cgccgcggtg cgagtctatt ccccgaccgt cgacgacccc gccttcccgc
    21181 ccaacgcgcc ggtggtgaag gtgccggctt tcccgatccc cggccgctcc gaatatcgcg
    21241 cgcccttcat gctgccgcgg cgcgcgaaga aggacctccg cgcgttccgg cccaacatcc
    21301 tccacatcgc cagccccgaa gtgctcggcc accgcgcggt cacgctggcg cacaagctcg
    21361 atttgccggt ggtcgcctcg gtccataccc gcttcgaaac ctatccgcgc tattacggcc
    21421 tgtcggtgct ggagccgctg gtcgaggcgg cgctgcgccg gctctaccgc cgctgcgacg
    21481 ccattttcgc gccgtccgat tcgatggccc agctgctgcg cgagcagcgc atgaactata
    21541 atgtcggtat ctggacgcgc ggtatcgacc gggagatctt caaccccgcc cgccgcgacc
    21601 tcgaatggcg gcgcgggctc ggcatcgacg acgacgtgcc ggtgatcggc ttcgtcggcc
    21661 ggctggtcat ggaaaagggg ctcgacgtct tttcggagac ggtcgacacg ctcgagcggc
    21721 ggcaggtgcg gcacaaggtg ctggtggtcg gcgaagggcc ggcgcgcgac tggtttgaaa
    21781 aacggctgcc gaacggcatc ttcgcgggct tccaggaagg gccggacctc ggccgcgccg
    21841 tcgcctcgat ggacatgctg ttcaacccgt cggtgacgga gacgttcggc aacgtcacgc
    21901 tggaggcgat ggcagtcggc ctgccggtgg tggcggcgat cgcgaccggc agcgaaagcc
    21961 tggtcgagga ccgcgtcacc ggccgcctga tccgccccgg cgcgatcgag gccttcgccg
    22021 acgcgctcgc ccgttattgc ggcaacgagg aggagcggat cgcggccggc caggccggcg
    22081 cccgggtcag cctccgctac ggctgggacg aggtcaacca gtcgctggtc gacgcctata
    22141 tccggatcat ccgccagcgc gccgccggcg cccgcatgaa gcggagcccg gtcccgtgag
    22201 cgccgccatg gtcgcggggc cgccgcggtg agcgacctct tcggagatca ggcggccgcg
    22261 ccggcgccgc cgcctccgga caccgcgccg ctcgcggatc gcctgcggcc gagatcgctc
    22321 gacgaggtgg tcgggcagga gcatctcacc ggccccgaag gcgcgatcgc ccggcaggtt
    22381 gccgccggca agctcgcctc gatggtgctg tgggggccgc cgggcaccgg caagacgacg
    22441 atcgcgcggc tgctcgccga cgcggtcggc atgcggttcg tggcgatctc ggcggtcttc
    22501 tcgggcgtcg ccgatttgaa gaaggtgttc gccgaggcga aggagcatgt ccgcgccgga
    22561 cggcagaccc tgctgttcgt ggacgagatc caccgcttca accgcgccca gcaggacggc
    22621 ttcctccctt atgtcgagaa tgggacggtg gtgctggtcg gcgccaccac cgagaatcct
    22681 tcgttcgagc tcaacgcggc tttgctgtcg cgggcgcagg tgctgatcct caaccggctc
    22741 gatgcgcgcg cgctgggcaa gctcctcgac cgggcggagg cgctgatcgg gcgcccgctg
    22801 ccgctgacgc ccgaggcgcg ggacgcgctg gtcgcgaccg ccgacggcga cggccgcttc
    22861 ctgctcaacc aggcggagac gttgttctct atctccctct ccccttcagg ggagagggtg
    22921 ccgagcgaag cgaaggcggg agagggggag ttcggagggc aggatgccga cggactcccc
    22981 ctctccccaa ccctctcccc tggaggggag agggggaatt tgctcgacgc cgccgcgctc
    23041 tcggccctgc tccaccggcg ggtggcggtc tacgacaagg accgggaggg gcattacaac
    23101 ctcatctccg cgcttcacaa agcgatccgc ggctccgatc cgcaagccgc gctctactat
    23161 ctcgcccgga tgctggtggc gggcgaggag ccgctctacc tcctccgccg gctgacccgc
    23221 gccgccgtcg aggatatcgg cctggccgat ccgcaggcgc tcgtgcagtg catcgccgcc
    23281 aaggacacat acgacttcct gggctctccg gagggcgagc tcgcgatcgt gcaggcctgc
    23341 ctctatctcg ccaccgcccc caaatcgaat gcgacctaca tggcgcagaa agcggcgtgg
    23401 cgttcggcca aagagacggg atcgctgatg ccgcccaaga acatcttgaa cgcgccgacc
    23461 aagctcatga aggaggtcgg ctacggcagg ggttatacgt atgatcacga cgctcccgag
    23521 ggcttttccg gcgacgatta ttggccggag gagatggagc cccaggcctt ctaccgtccg
    23581 accgaccgcg gcttcgagaa gcggatcgcc gaacgcatcg cctggtggga agagcgccgt
    23641 gccgagctgc ggggctgagg ccgccgtcat tcccgcgaaa gcgggaatcc cgcttctgac
    23701 ggtcgccgca aggagaagta gcgggatccc cgctttcgcg gggatgacga gagtggcgac
    23761 ctgcctcgcc ctcgtcgcgc tggcggcgcc gggcgcggcg caggtcgatt ccgctcccta
    23821 cacccgcgcc atcgcggccg gctacaaagc cagcttcctg tgcagcgaca tcttcaacgc
    23881 cggccagagc gaggcgatcg tcgagcagga cgatctccag cgcacctatg ccgagctggt
    23941 gccgatcatg cgcaacctga agccggtgat cgaccggcag gcgaagacgg tgagcgtcgc
    24001 cttcgacgac aagctgccgc cccggatcgc ctcgtggcgc ccgcatctcg gctgcgcgca
    24061 gctcccgatc ggcgcgcgca tggaaagccg ctccgccctg ccctcgctca gcgttgaccc
    24121 ggcggtcgac cggtccgacc gcctcccctg gccgaccggc gaccgcaacg ccgagggcaa
    24181 gccgcgcggc gacgcggcgg cgctgcgcca actcgtcgcc gccgcgttcg accggcgcag
    24241 ctacggccag ggcagcgaga ccacggcctt ggtgatcgtc caggacggca ggatcgtcgc
    24301 cgaacgctat cgggcggatt tcacgcggca cacgtcgcag cggacctggt cggtcgccaa
    24361 gagcatcgcc ggcacgctgg cgggcgtggc cgtccagcag cgccggctcg acgtcgacgc
    24421 gcccgccccg atccccgaat ggcaaagccc cggcgatccg cgcgcggcga tcaccaccga
    24481 ccagctgctg cgcatggcga gcggccttca cagcgaccat gccggcaacc gcaccgacgc
    24541 gctctatttc ggcggcaccg cggtcaccga gcaggcgacc ggctggccct tggaggcggc
    24601 gccgggaacg cgcttccgct acgccaataa cgacaccctg ctcgccgtgc gcgggctgcg
    24661 ctcggcgctc ggcgaggaac gctacctctc ctttcccttc acggagctgt tctggaaggt
    24721 cggcatgacc cggaccgtcc cggagaccga ctggaagggc aatttcatcc tgtcgagcca
    24781 ggtctggtca accgcccgcg acctcgcccg gcttggcctg ctctacctgc aagacggcgt
    24841 ctggaacggc gagcgcatcc tgccgccggg ctgggacgat tatgtgcggc gccacggccc
    24901 ggctcagccg gccgcgggcg gcggctatgg cgccaccttc tggaccttcc ccacgggcaa
    24961 gggcctgccg gaggacgcct ccgtgatgcg cggcaatcgc ggccaatatg tcgtcataat
    25021 cccctcccgc agcctggtga tcgtgcggcg cggctatgac ggggcggaaa gccagttcga
    25081 cctcgaccgc ttcaccgcgg accttctcgc cgcgctccgt tgagcttgct cgggaaccag
    25141 cccgccgatc agtcatttct tacggttgcg gcacgatttg cctgatcttc ctcgaaaggg
    25201 cctgcaacca tgtaccagag cgatcccggt tcactttact ggcaggggca ggtccaggaa
    25261 tggctccccc gcatcgtcgg cgggctcgcc atcctcatca tcgcctacat cctagcgcgc
    25321 gccgccaaat gggcgatcgc caaggtggtg gaccgcgtgc cggcgctcaa gaagcatcat
    25381 gaggcggagc cgggcaagac gctcggcagc ctgatcggcg acatcgcttt ctggctgatc
    25441 ctgctcatcg gcatcatgct cgcgctgcag ccgctcggcc tcagccaggt gctggagccg
    25501 gtgcgccagc tcaccaccaa tgttttcgcc ttcctgccga agctgctggc ggccgggctg
    25561 atcttcttcg tcggaatggt catcgccaag atcgtccgcc gactggtcga gggcgccctg
    25621 ctcgccgcca acgccgacgg ctggctgcgg cggaccggat tggtcgagac ggcgggcacc
    25681 gctcccgcca cgcccgcggg cgggccggtc accaccacca gccgcgcttc gatcagccgt
    25741 tcggtcggaa cgctcgtctt cttcgtgatc atgatcccgg tcgccatcat ggccctggac
    25801 acgcttcagc tcgaggcgat ttccgggccg gccacgcaga tgctgcggac catcctcgac
    25861 gcgatcccgc tcgtgctcgg cgcgctgatc ctgctcgcca tcggctattt cgtcggcaag
    25921 ctcgccaagc aggcgatcga gcagatcctg ccttcgatgg gcttcgaccg cgcggtcggc
    25981 gcgctcggcc tctcgcccgg cagcaccacc ccgtcgcgca ccgtcggcac gatcgtgatg
    26041 ctggcgatcc tgctgttctt cgcggtcaag gcggccgaat tgctccagtc gccgatcatc
    26101 gccgtcatgc tcgcgcagtt gctcgagctc ggcggccgcg tgctgttcgg cgccggcatc
    26161 atcctcggcg gcttcgtctt cgccaagatc gcctccaacc tcgtcgccgg caccggcagc
    26221 gagggctggc tgccgcaaat cctcaaatgg tccatcatcg ccttgtcggt ggcgatgggc
    26281 ctgcggttca tgggtctcgc caacgagatc gtgatcatcg ccttcgcctc gatcatcggc
    26341 tcggcggcgg tcgcctgcgc gctcgccttc ggcctgggcg gccggccgac cgcgcacaaa
    26401 ttgctcgagc gctggaccga ggccaaccag gtgccggccc cgccgcgccg gccgcgcacg
    26461 ccgaccccgc ccgacggcac ccagccgcct ttggtctgag ccgctggccg tcgttgcggg
    26521 gagcggcgcg ccgcgcccgc aatgacggcc gcccccacac ccttcacccg ctttgccgcg
    26581 atcgactggt caggcgccaa gggccggcgc cacaagggca tcgccgtggc cgtctgcgag
    26641 gccggcgggg gcgcgccgcg cctggtccag cgcgcggcgc cttggtcgcg gaccgaaata
    26701 ctcgactggc tgctcgccac cgctgcggag gcccctaccc tctacggctt cgacttcagc
    26761 tacgccccgc cgatcgtcga gcgcggcgcc tatctgccgg gcgaagcggg gccgccgtcc
    26821 gacgccaaat ccttctgggc ctatgtcgat gggttgtgcg aggacgagga tctcggcgcc
    26881 gccagcttca tggagcggca tcaccggcgc cacttctatt tcggcgcggc cgacggggtg
    26941 aaggccgatt tcctccacca ccgcgtctgc gagcatgcct tcaacgccct cggcggcggc
    27001 aaggcctcga ccctctacga cgcgatcggc gccgcgcagg tcgccaaggc gagctatgcc
    27061 ggcatgcggc tgctccaccg gctcggcggg cgggtgccgg tctggccctt cgacttcgct
    27121 caggacaggc cgatggaccc cgtccccgcc gccggcagcc tggtcgtcga aatctacacg
    27181 cgggtgttca tccgcctcgc cgggctcagc gggcgcaagg tgcgcagccg cggcttgctc
    27241 gacgaggcgc tggccggcct cggcagcggc cccgcctcac tggcaggcga gcccaacgac
    27301 catgagaccg acgtgatcat cgccgcggcc gggctgaggc gcatcgccga cgacccgctc
    27361 tactggagcc cgcccgcgct gacgccggcg atcgcgcgca ccgaaggctg gacgttcgga
    27421 gtgctgtgag gcgggggtgc cgcgctggtg ccgcgtgtcc gactcgaacg gacgacctac
    27481 cgcttacaag gcggttgctc taccagctga gctaacgcgg cgcgaacccg caaatgcgac
    27541 aagagcggcc gcgcgtccag cccccgccgc ccgcccggat ctgggacgac cgaccccgaa
    27601 ccccgcttgg tgagcggccc gcaacctgct ataggcgcga cgagccggtc tgtctggggg
    27661 atgcgccgtg gaagaagaac tcgccgaaga attgatcgcc gtggaagcgg aacccggtga
    27721 cgaggaagtc tgggccgagg agctcccggt cgaagcggcc gagtcgagcg acggcgaggg
    27781 cggcggatcg gacgagatcc ccgcggacga ggaaatctac actttgtacc ttgaggacga
    27841 ggttcccgcc gaagatgccg gcgacggctc cgacggcgaa gaagagctcc cggccgacga
    27901 agaaatttac accacctata tcgacgaggg cgaggttccg gccgatgagg gcccgggcga
    27961 ggatcccggc aacctcgaag atccggtggt cgacgagggc ggtaccgagg ttgtcgacgg
    28021 cgagggcaag gagatcggct cggtcgacgg cgagatcgac tatatcggcg gcggctggga
    28081 cgaattgctc tatccgacga tgaacgaagg cgaatgggcc tatactacca gcgccggcgg
    28141 catcgacgat ccgcgcatct atatgagcag cggctcaggc ccggccccga acgagcgcgg
    28201 cgaggaggct gcccaggtcg tggaggtcgc cacctggcac accgtcaacc tcgtccacca
    28261 tgctctggtg gccgatctcg cgccgcgcga gcatatcgcc tgagccgaat cggctaggca
    28321 aaaccggcta gggaaagccc cctcagacag gcggggccgg cgggcgccgg cccctcccct
    28381 tttatcagca ggtcagcgga gctggggccg cgccctcggc cggcgccttc ttgacgcctt
    28441 cgatgcaggc cggcttggcc gggctcgcct cggccttggc cccgagcggg gcttcggccg
    28501 cgccgccgcc cgaactattg ccggtcagcg cccacaggtg cgacttggcg acggtctcgc
    28561 cgctcttggt ctgcgccgcc agcaccgtgg cgacgccgct gcggtcggcc ggcacgtagc
    28621 gccgcagcac cgcgcgctgg aagctgtcga cgctggccga gccgcccgcg cccgcggcgg
    28681 cggaggcggc agcctcggcg gccagcgcgt cggtggcgcc ggcggcggcg agcgcggccg
    28741 ggtcctgcgc cttgcgcgcg gtcatcgaga ccaccggctc gacaccgcta tggcggtggc
    28801 ggaacgcctc gggccggccc cagccgttgc tccagcggta gaagatgtgg gtgccgacat
    28861 tggcgatctt ctcgaggctg gagctccagt aaggcaccac ccaattggtg tgataatggg
    28921 tggcgtggcc gaccggcgca tagacgtagc cggcgagcgc ggcctgcgcg acctggccgg
    28981 cgcgcgccca gaccgacgcc atcggcgtgc gggcgagcga gccgtcgcag gtgaaggtga
    29041 actggcagcc ggtgctgcgc tccgatccct gccacacgac gccgcagacg ctgctcggat
    29101 aggcggggtg gcggactcgg ttcagcacca cctgcgcgac ggcgcgctgg ccgtcgagcg
    29161 gctcggtggc ggcttcgtaa tagacggcgg cggtcaggca ctgcagcgcg cgggcgcggt
    29221 ccgattcggt cctggcgagc aaagcgaagg cggtcgcggc cggattggcg gcggccgaca
    29281 gcggcaccgc cgcgttgatc gcgaccgcgt cggtcggcgc gacctgcttg aactggagcg
    29341 gctcggcctg cgggagcgtc ttgcgcacgg cttcgggcag gttgccgctg acccgcgcca
    29401 gcgcgcggcc gtcgggcccg ccgaggccga ccacgctgag gccggcgagc aggtaaagac
    29461 aggcgagggt cgcgagcacg atggtcgcgc cccaatgctt tcgttccaga ctaggcatcc
    29521 ggagcaccgg catggtgaat gcgggcatcc ggaacgcagg caagcgcaag gcagctgtca
    29581 gcatgaacac tccaaattgc gggcgcgccg atagagcggc acggccatcg agacaagtca
    29641 cgtccggccg gccaatggcg ccaaacgtcc gaatcaaacg ggtatctgca cacgccccgg
    29701 ctgaagctca tagcataagt agctgtacct agccaaaacg ggccgcctcg ttccggcctc
    29761 cgaacgaccc ggaacctcgc aaagccgcgc ccgttgagcc ggcagaacga actgatggaa
    29821 cccgctttga tggccaccca agcccgcgtc ccggctcatg tcgagccggc gcaattcctg
    29881 accgacagct tccagcgcgc ccttgcccat tggaaccatg cccggctggc gccgcaattt
    29941 cccggcgccg attggcaaaa ggcgctggac cgcgacgtcc agatgctgcg cctcgaaggc
    30001 ggcctcctgg aagggctgcg cgccgaggtg gtcgccgaag cggcggccgc gccgaccgac
    30061 gccgacggct tcatcgcctg gttcgaagcg ctgaaggaca gcggcccggg tcagggcgac
    30121 ccgcttttcc cgtggctggc cggtgaggcc tcgctcgaag agatgcgctg gttcctgacc
    30181 caagaagcgg cgggcgaagc cgggttcgac gatctcgtcg cctacaccca ggtcaagctg
    30241 ccgacgcgcg ccaagctcga gctcgcccgc aattattggg acgagatggg ccgcggcaat
    30301 cccaagggca tgcacgggcc gatgctggaa gtgctgatcg acacgatggc gatcgagccc
    30361 gacatcgaca ccaccgcctg gcagagcctg gcgctggcca atgcgatgac cgcgatggcg
    30421 accagccgcc gctatgcctg gcattcggtc ggcgcgctcg gcgcggtcga gctcaccgcc
    30481 cccggccgct cggccgcgac cgccgcgggc ctgcgccggc tcggcttttc cggccgcgac
    30541 cgccgctatt tcgcggtcca cgcggtgctc gacgtcaagc atagcgagga ttggaaccgc
    30601 gaggccctgc gcccgctggt cgaggaggat ccccgccgcg ccaccgccat cgccgagggc
    30661 gcgctcatcc gcctcaattg cggcgcccgc tgcttcgaac gctaccgcgc ggaactctgg
    30721 tagcggagcg ggagacgcgg aggcggctga agccacggaa agccaactgc cgctcgagca
    30781 tccgttcgtc ccgagcgaag tcgagcgacc ccgccgagcg aagccgaggc tgtgcgatcc
    30841 cgatctcgac ttcgctcgaa agggtgtctc gactgcgctc gacatgaacg gatttcacag
    30901 cccaatcgag gcagcacccg caaccttccc ccccggggga cccctccccc cttggcccac
    30961 ccgcctccgg ccgctaaagc tcccctcatg gccaccatcg caccccccgc tcccccgccg
    31021 ctgctgctgc cgggcgacca cgcaccctgg ttccacgcgc cggctctgtc gggatcgccc
    31081 aattacgcgt tccagaccgt cgccggccgc cacgtcatga tgctgttctt gggctcgacc
    31141 cggatcgaag cctgcgccgc ggcgctccgg ctggtgctcg agcggcgggc gatgttcgac
    31201 gacgtcaatg cctgcttctt cggcgtcacc gtcgaccgcg aggatgaggc caagggccat
    31261 atcgcccaat tgctcccggg gctgcgcttc ttcctcgatt acagccagga ggtcagccgc
    31321 ctctacggcg cggtcggcaa ggccggcgag ggcaatgtcg aatatcggcc gcactggctg
    31381 ctgctcgatc cgggcctgcg cgtcgtcgac cgcttcccga tcgaccgcgg cggcgaggcg
    31441 ctcgacgcgc tccagcacgc catcgcggcg cccgccggcc ccgcatgggc gccggtgctc
    31501 gaagtcccca acgtgctcga gcccgagctc tgccacgagc tgatcgcgct ttacgagggc
    31561 gaaggcggca ccgaatccgg cttcatgcgc gacgtcgacg gcaagaccgt gctgatcgtc
    31621 gatgccgccc acaagcgccg cagcgactgc atgatcgagg acgaggcgct aaagcggcgc
    31681 ctcgtcgcgc gcatgaaccg ccgcctgagc cccgccctca aaaaggcctt ccagttcgag
    31741 gcgacccgga tcgagcgcta cctcgtcgcc tgctacgacg cgtccggcgg ctatttccgg
    31801 ccgcaccgcg acaacaccac caagggcacc gcccaccgcc agttcgcggt caccatcaac
    31861 ctcaacgctg gcgactatga aggcggcgac ctgcgcttcc ccgaatatgg ccagcgcacc
    31921 tatcgcgccc cgaccggcgg cgcgatcgtc ttctcctgct cgctgctgca cgaggcgacg
    31981 ccggtcacca gcggccgccg ctatgccttc ctccccttcc tctacaatga ggaagccgcc
    32041 cgcgtccggg agtcgaacaa cccctatctc ggcgaaggca taggcgagta ccggatcggc
    32101 tagccgccgc ccccgcccgt catgagcctg gaatccgtcc gcgccgacct cgccgcacgc
    32161 gcgcccgacg tgccgatcat cgagatgggg caaagcaccg ccacggtcga gctggccgcg
    32221 caggcgctcg gcgtcgagcc gggccggatc gccaagacgc tcgccgtccg cgtcggggcc
    32281 gagaccttcc tgctggtcgc gcgcggcgat gcacggctcg acaaccagaa gaccaaggcg
    32341 gcgttcggcg gccggccgcg gctgctcggg ccggaagaga cgctcgcgct caccggccac
    32401 cccgtcggcg gcgtgtgccc gttcggcctc gcgacgccgc tcccggtcta ttgtgacctc
    32461 tcgctccagg cctatgacct ggtctatccg gccgcgggct cgctcgaaag ctcggtgaag
    32521 ctgagccctg cccggctcgt cgaattggtc ggcggccgct gggtcgatct ctgccgcctg
    32581 ccgggctgaa ccgcctcgat caagcgaccg acggcgccgc ctccacccgg cggcgcgagc
    32641 gcaccgccgt cactgctttc ccgatcgcgg cgaggctgcg gtcgatgtcc gcgtcggtgg
    32701 tcgcccagga cgaaaccgac agccgcaccg ccgcccttcc ctgccaatgg gtcggtccga
    32761 accagcattc gccgccgcgc tcgacctggt cgcggacgcg cgcggtgaaa tcctcgtcgc
    32821 cgatcgaggc gacgacctgg ttcaacacca cttcgttgtg aatctcgaag ccgatcgcgc
    32881 gcagcccctc ggcgaggcgg ccggcatggc cgcagcagcg ttcgaccagc tcggccacgc
    32941 cctcgcggcc gagcgtccgc agcgccgccc acacaatgat gccgcgcgcg cgccgcgaga
    33001 aatcgggcac gaggtccttc atcggcacct cctcgctggc cggcatatag gtggcggcaa
    33061 ggcccatcgc gccgcgcagc gcgtcggcgt ggcggcagac gatcatgccg ctgtcatagg
    33121 gcgtgttcag ccatttgtgg ccgtccgtta cccagctgtc ggcaccctcc accccttcca
    33181 cgaggtgacg gtggcgcggc gaggcggccg cccacaggcc gaaggcgccg tcgacatgca
    33241 cccaggcgcc ggcggcgcgg gcggcggcga tggccgggac gaacgggtcc gacgcgcccg
    33301 aattgacgtt gccggcctgg aggatgagga tggtgcggtc gtcgagcgct ggcagccggt
    33361 cggcgcgcag ccgcccctgg ccgtcgaccg gcaccagttc gacgttgccg cggccgaagc
    33421 cgaggatgcc gagcatcttg agaacggtcg aatggacctc ctcgcccgcc accaccctca
    33481 gccgcggcgc gcccgccagg ccgtcggcat cgacgtcatg gccgagccgg agcagcaaat
    33541 ggcggcgcgc ggcggcgagg ccgaccaggt tgccgatgct ggcgccggtg acgaagccgg
    33601 tcaccgattc cggcggcagg ccgagcatct ccaccaccca gcggccggcg acgcactcga
    33661 tccgggtcgc cgcgggcgag ctcaccggca tggtcccggt ctggtcccag gcggcggcca
    33721 gccagtccgc ggcggtggcg accggaaggc ttccgccggt gacgaagccg aaatagcggc
    33781 cgccggtggt cgccaccgtg gccgggctgc cggcgcggtc gagcaggcgg atggtgtcga
    33841 gcgcgtcgac tggccggtcc ggcagcggct cgtcgaacgc ggcgagcccg gccaccgccg
    33901 cggcgtccgg gaacacccgc cgccgatgcg ccttcgcgat atagtcgagc gcccgttcct
    33961 gcgcgatgtc gaacaacacg tcgtccatga tctgcccctc gtctgtcgtc aggccgcttc
    34021 ggccagcgtc ccggccagcg cgggataatc ggtatagccg cgcgctccgg agctgtagaa
    34081 ggtcgcccgg tcggcctcgg ctagcggagc gccgctcgcc agccgcgccg gcaagtccgg
    34141 gttggcgatg aacgcggtcc cgaacgcgat caggtcggcg cggccctcgg cgaggtcggc
    34201 ctcggcgccc gccttgtcgt agccgccgtt gaggatcagc cgcccgcgat aatggccgag
    34261 caccagctcg gcgacgtcga agcccggccg cgtgtcgtag acgtggagat aggcgaggcc
    34321 gagcgcgtcg agccggtcga gcagataggc gtaggtggcg gccgcgtccg cctcgacgat
    34381 gtcgttatag ccattgccgg ggctgatccg gaccccgacg cggtcagcgc cgatcgccgc
    34441 ggccgcggcg gcggcgactt cgatcaggaa gcgggcgcgt ttttcgatcg agccgcgcca
    34501 ctcgtcgccc cgcagattga cgttggtggc gagaaattgg tgcggcagat agccattggc
    34561 gccgtggatc tcgactccgt cgaagcccgc gcggacagcg ttgcgggcgg cgcgggcaaa
    34621 gcctgcaacg atgttcgaaa tctcgcctgc ttcgagcgcg agggggacga cgtggtcggc
    34681 cccccccacg gtcgaagggg cgaccggcgt gcggccatcg agcaaggcgg gatgggagat
    34741 gcggccgaca tgcatgagct gcgcgaagat gcggccgccg cgggcgtgga cggcgtcggt
    34801 cacgcgctgc cacgcgcgca cctgcgcatc cgtgtagagg ccgggcgagt cgacatagcc
    34861 gtggcccatc ggctcgggcg cgatgccttc ggtgacgatc aggccggccg tggcgcgctg
    34921 ggcgtaatag gtgcggacga gctcggtcgg caccgcgccg ggcgagcggt cgcgggtcat
    34981 cggcgccatg acgatgcggt tccggagcgc gtagcggcca agccggacgg gggtgaagag
    35041 gtcggtcaca ttcgcttctc ccaggtcagg ccgcgagcgg cgcggcgatg aggcggtcga
    35101 tgccctgaag gtcctgcccc ggcgtccagc cgcggctgcc gagcaggcgc caattcatgc
    35161 gcggataggc gccgatctgg cggccgagcg cgccggtgat gagcgtcatc cgctcgatcg
    35221 cgcgggcgtt gcggagcggt cctgcgtcga ggtaccggaa cggcgtccct tcggcgaggc
    35281 cgagcaattc gcgcttcgcg gcggcatcgt cgccggtcac cagcacgtcg ctcgcgcctt
    35341 cctcgaacag cggccggtcg aacacttccc agaacacgtt cttgtaagcg ccgaccaccc
    35401 gcgtctcggg gagccgcttc tgcagctcct cggcgccgct cgaatcccag ggcgtgatga
    35461 agtccgaata atcctcgttg aaggggttgc tgatgtcgat caggagcttg ccgcgcagcc
    35521 gcttcgcaag cgggccgacc tggtcgagca gtccgtcgcg caggaacacg gccggaagga
    35581 aagccccggc cgcggcggca tcctcgtacg agccggggcg gagtccgtcg aggttcagcg
    35641 cctcggcgat ataggtcgcc cgccggaggc tgcgcgatcc caaggtgacg gtgcggccgg
    35701 cgcgggcgaa catcgccgcg aggcgcaccc ccatcctgcc ggtgccgatg atgccgatgt
    35761 cgctttgctg tgccatggag cggcagatgc gccgatcggc ggcgtccgac tagccgccga
    35821 ttacgggagt caccgttcag gctattggac tatcgccgag cgcggcagcg aggtgatcgg
    35881 cgaacgcttt gacccgcggc gtcatgtcgc gccgcgatgg gaagaccagg tgcagcgccg
    35941 aaggctcgct gttccagccg tcgagcaccc gcaccaaagc cccgctcgcc acctgcggcg
    36001 tcaccagcgc gtcgggcagc gcggtgatgc cggcgccgcg cgccgcgagc cggcgcagaa
    36061 aggcgaaatt gtcgcaagag atgcgcgccg cccccgcccc gaccggatcg gcggcgcgca
    36121 gcacccggct ttgcgagggc gagctgaaca ggaggaggtt gtggtcgacc agcgcggcga
    36181 ggtccgcggg gcggccgtgg cgggcgatgt agctcgggct ggcatagagg ccgaacacga
    36241 tctggccgac cttgcggatg atgaggccgg ccccgccggg atcgccgccg cgcaacgcga
    36301 ggtcgatatt ctcggcgacg aggtcgaccg ggcggttggt gagggtgatc tcgaactgca
    36361 ccttggggtg gttctcgagg aacgaacaga tcgccgtcgt cagcgcatcg cgcgggaatt
    36421 cgagcggggc ggtgaagcgg atcgggccgg acagcacgcc gtcctcggcg gtggtggcgg
    36481 cctcggcgtc gaccaaggcg tcgaccgcgc aggcgaccgc atcgaaatag gcccgtcctt
    36541 ccgaggtcag cgcgacctgc cgcgtcgatc gcttcagcaa ttgcgcgccg agccgcgcct
    36601 cgagcgcctg gacgcgcgcg ctgaccgtcg acttgggcag atgcagccgc cgccccgccg
    36661 ccgcgaaccc cccggcccgc accacttcca cgaacacccg cgtctggttc agatccatac
    36721 ccgtcgtcca accagccgga cggcgcgtcc ggcggcgcca ggatatcgag ctttgacgaa
    36781 cctgtcagcg gtgccggcgg accaggttcc ggagcctgcc gtcaccgccg gccgaagtcg
    36841 atgcggccga tgagccgttc gtcctcctca tcgtccggca ggagctcgat gtcgattacc
    36901 tcctccgtct ccgtcggccg gccgaaatcg cggatcagcc gcgcggtcac ccgcgaggcc
    36961 ccgttcgggt tgagggcgtc cgacgcgtag gcgtcggcgc gaacctcgaa ccggccggtg
    37021 ggcgcccgcc gcaacagata ttcctccggg ccatagccgc cggtcatgtc gttggagagg
    37081 cggccgccga tcgctgtgcg gggattgttg tagaccgccc gctccccgct cggctcatcc
    37141 acccacaggt cgacgtcggt ctcatcgctg ttccattcga tgaggacgcg catgtcgacg
    37201 tcgagcagcc ggatcaggcg cggatcgagg gcgagcggcc cgccgcccaa cgcgcggtag
    37261 cgggggatca gccggttgac gtccatcagc gcgattgcct cgatcccctc ataatcctcg
    37321 tcccacggcc gcatgatcac ctcggtcagc agcgaaatcg cgcgctccag gtcggcgcgg
    37381 gcgggctcgc cggccagcga gcggctgcgc ctggccagcg cgagggccag cgcgcgccgc
    37441 ggctgtgggc gatcgggctc ggccgccagc aagcgctcgt agaggaatat cgcccggtcg
    37501 atctcattgt agcgcatcag ccgctcggcc acgatcgcca gcgtctcgct gttgcgcgtg
    37561 gcgaggtcga gcgcggagag caaaagcgta accgcctcgg cgcggcgccc cttgcggtga
    37621 taatattcgg acacgtcgag ccagaaggcg gggagctcgc catgcttcgc ctgctgcgca
    37681 gccagcacgg cctccagcgc ctgcggcgag gcggcgtcga gcgcggcgag ataggggcgg
    37741 tccggcgccc acgcctcgac ctggatgacg gcctggttgc ctggcctctg tccctgactg
    37801 ccggcagcgc ccgcgctgtc ggcattggtc acggtaaccg gcgcagcggc catcagattg
    37861 gggctcggga tgcgtgagcc ggtaacggtg atcgcctcgt caccctcagg gggcggcgga
    37921 ggtgggggcg gcggcggagg cggagctacc cccaccgaaa gctccgcttc cggtggccgc
    37981 ttggagcgcc gcgcccgcgg gtcgaaccgc ttgttccacc acgcgacctg ctcttcccag
    38041 attgcgagca cttttccgag ccgttcctcc gcggcctgct gcttctcccc ggcgacctcg
    38101 gcgcgcagcg catcatattg ccgtcgctct tcctttggat agccggcggg cggatcgatt
    38161 cctgcattgg cataatcttc cggcgtctcg agcaccacga acgagacatt ggggctgccc
    38221 accgaatagc gccgcgagaa ggcgatcaat gcctcccggt ccaatgcgtc cgatgccgcc
    38281 cgcagggccg cgcgatcagc cgcccagagc gagcccgcgc cggcgagcgt cggcaccgcc
    38341 ccgccggcga gcggataacg acgctgcgcc gcgccggcct cgacgcccgc caaatccacg
    38401 atcagcgact cgccctccgg agcctgtccg acgatccgcc agccgccctc ggccgcgtcg
    38461 agttgcacga actccaccgg ctggccgctg gccgagcgca cccccgccac ccgcggaacc
    38521 gcccgcgtga gcgactcgag cacgtggtcg gcgctggttc cgatgagatc ataggcccct
    38581 ccgccccggg cgatgtcgcg cagccacgcg cggtcggcgt cggccgcggc cgaaatcgcg
    38641 gacaaagggc aagcggggcg aaagtcgccg cgccggtcga tggtggcgag gccgtccgag
    38701 aatagcaggc aggcgtcggc gggagcgccg ccgccgatca cggcgaagct ggtcgcgccg
    38761 cggtagcgga cggtcttcag ccgcgcgacg acctcgtccg cgccaaggct cagccgttcg
    38821 gccccgccgc tgtcgaacag gatgagctcg atccgcgccg ggcgcacgcg ctgcaggtag
    38881 cgccggagca gcgccagctc gcggccatgg tcgtcgtcag cgcgcgagag cgagcggtcc
    38941 cagaaaatgg ccaggctgcc gggcctggca taagcctcgc cggaagcggg cgcggcg
//
LOCUS       DASVRK010000177         7574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_33298, whole genome shotgun sequence.
ACCESSION   DASVRK010000177 DASVRK010000000
VERSION     DASVRK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33298"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..447
                     /locus_tag="VF589_06965"
                     /pseudo
     CDS             88..447
                     /locus_tag="VF589_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(652..819)
                     /gene="rpmG"
                     /locus_tag="VF589_06970"
     CDS             complement(652..819)
                     /gene="rpmG"
                     /locus_tag="VF589_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX8257357.1"
                     /translation="MAKPATIKIKLVSTADTGFFYVTKKNPRNTTEKMTFRKYDPVVR
                     KHVDFKEAKIK"
     gene            complement(950..1867)
                     /gene="cysK"
                     /locus_tag="VF589_06975"
     CDS             complement(950..1867)
                     /gene="cysK"
                     /locus_tag="VF589_06975"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX8257358.1"
                     /translation="MKAASILETIGDTPHIRINRLFGDAEVWSKSERANPGGSIKDRI
                     ALAMVEDAEASGALKPGGTIVEPTSGNTGIGLAMVAAVKGYRLILVMPESMSLERRRL
                     MLAYGAEFDLTPKEGGMKAAIARAQELVAATPGSWMPQQFENEANIAVHVRTTAQEIL
                     RDFRDAPIDVIITGVGTGGHITACAEVLKQEWPGLKVFAVEPTLSPVISGGQPGPHPI
                     QGIGAGFVPKNLHVDAIDGVILVDPADAKEMARRAAREEGLLVGISSGATLAAIAQKL
                     PELPAGSRVLGFNYDTGERYLSVPDFLPE"
     gene            1978..2523
                     /locus_tag="VF589_06980"
     CDS             1978..2523
                     /locus_tag="VF589_06980"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8257359.1"
                     /translation="MTPIHHDCLDAFSAAECDAMIAIAAADAGEAGPVWGGAGYALDP
                     AVRSVRTVFRARSEETGWIYDRLDRLFGEAAERLGVAVAPVSEDIQIMAYGVGNHFQV
                     WHSDAGTDLQARRILSVSVELSEPGDHDGGDLEIVPVAIGRRTLPRGAARLFLSRALH
                     RVTPVTRGTRHSLVSWTGAPG"
     gene            complement(2544..3878)
                     /locus_tag="VF589_06985"
     CDS             complement(2544..3878)
                     /locus_tag="VF589_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8257360.1"
                     /translation="MPAASPFHYRDYRAFWLARLASTIGSTMLVVVIGIHVYDTARLT
                     MGIKEASFWLGMVGLAQFLPLFLLTLVAGYIADRIDRRWIVRASIALELGCAAALAAF
                     VWADSISLAPLFAVAVALGIGRAFAGPALASFAPNLVPKEILPSAIALNSIAWQAGAV
                     AGPLLGGAAYALRIALPYEVSALLFALAIGAFMVIRPIPRGNAGASHPLRAVIEGLAY
                     VRNNKIVLGAITLDLFAVLLGGATAMLPVYARDILHVGSEGLGALRAAPAVGAALTAL
                     ILARRPLRRQVGVKMFVCVAVFGLATIVFGESRVLWLSLASLFVLGAADMISVYVRSS
                     LIQLHTPDAMRGRVSAVSGLFISASNELGEFRAGLAGAAFGPVVAVVGGGALAVVVTG
                     LCAWAFPSLRRADRFVAADEAVLADQPGKADAETELPAMVAGLNPKAEKGVP"
     gene            complement(4054..4467)
                     /locus_tag="VF589_06990"
     CDS             complement(4054..4467)
                     /locus_tag="VF589_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257361.1"
                     /translation="MSDSPSDPYTFEPIPSATRRRDGWTPERQRGFIAALARIGMVEA
                     AAASIGMSRKSAYALLERAGPDSGFAAAWAAAQQQGRGNAFLAAAERSGGVEVPHFYR
                     GVRCGTDRVYNDGLLLAALQAGHAQGWDRRGPGVG"
     gene            complement(4671..5246)
                     /locus_tag="VF589_06995"
     CDS             complement(4671..5246)
                     /locus_tag="VF589_06995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX8257362.1"
                     /translation="MMASAPPPLNILAIAGSLRAASYNRALLRAAQELAPEGMAISIF
                     DLADVPLYNGDVEAAGDPEGVARFKQAISAADGVLMATPEYNHGVPGVMKNAVDWASR
                     PPGQAPLGGKPVALMGASPGITGTARGQSQLRQAFEFTNSYVMPQPELLVFKAHEKFD
                     ADGRLTDETTRRYLQRFLTAFAAWVRRFQGG"
     gene            complement(5496..5915)
                     /locus_tag="VF589_07000"
     CDS             complement(5496..5915)
                     /locus_tag="VF589_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257363.1"
                     /translation="MDSVELMQIASGKDFWVGASSSIVGASIIGIVVYVFRNALSLSR
                     AQREKRRLEDQIFEQALDSSHSLAPFAYGIVQARALRYFLLAVFVAYVGDILSFLWPF
                     NSIAYVLSLWFIFQGLRWLYKIEKSAMKFLRRDTPRL"
     gene            complement(5981..6307)
                     /locus_tag="VF589_07005"
     CDS             complement(5981..6307)
                     /locus_tag="VF589_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257364.1"
                     /translation="MATMMDGQRIIMSGVVLSNGDSEVVISEGGQTLRFRFEQNDDSI
                     SGTGEPEDISILLPAGREAGSIASGFGLARDHDRVMVNYRVDWVLPSMRQIIYTVYEE
                     QQLEGW"
     gene            complement(6325..>7574)
                     /locus_tag="VF589_07010"
     CDS             complement(6325..>7574)
                     /locus_tag="VF589_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX8257365.1"
                     /translation="PYRIVGGFRFYERAEIRDALAYLRIIAQPSDDLAFERIVNTPKR
                     GLGDKAVAKVHGFARAAGVSLFSAAAQILDTDELTAAARKSLANLVADFARWRRMLSN
                     SSPAFAGEGDQPQAGGGASSGGGAFSDGGASSGGASPAGGLPHAELARLVLDESGYTA
                     MLQADRSAESAGRLENLTELTRAMEEYDTLGAFLEHVSLVMENDAARDEEKVTIMTIH
                     AAKGLEFGTVFLAGWEEGVFPSQRSLDEGGQASLEEERRLAYVAITRARHKCFIMHAA
                     NRRIYGQWTSSIPSRFVAELPEPHVDHQTTMSGGESLWRANWSERADPFADVARGTGR
                     GPGWRRAVTGGFERTPPRVIEARASAVSLGNPGRTDIALGQRVFHSKFGYGKVAEIEG
                     NKLEIDFEQAGRKRVLDSFVSLE"
BASE COUNT         1259 a         2351 c         2761 g         1203 t
ORIGIN      
        1 cgagcgtcca ggccagcgcg gacagggcga gggcttccgg gtcgggcggg tttgtgagat
       61 tcttgagcat gccgtaggga agggcgcgtg ggacaaaaaa ttctcgttgt cgaggacaac
      121 gaactcaatc tgaagctgtt ctgcgacctg ctgcgcgccc atgccttcca agcggagccg
      181 gtgcgcgacg gccgtgaggc ggtggagcgc gcccgcgcct tcgcgcccga cctcatcgtc
      241 atggacatcc agatgccgca tatcagcggg ctcgagctga tcgagcagct caagggcgac
      301 gcgatgctga gcggcatccc gatcatggcg gtgacggcct atgcggcgaa gggcgacgag
      361 gaccggatcc gggacgccgg agccgagggc tatgtgtcga agccgatctc ggtcatccga
      421 tttatcgagg cggtgcaggg gctgctgagg gcggtggcgg agcgggaggc ggaggctgtt
      481 ccggaggcgg cggacgagcc cgagcctgcg gcggaagaag gcgccgaggg tgaagtggaa
      541 gtcggtgcgg tgggtgagcc ggagctcgga tcaggcgcgg atgtcgagat ggatccccgc
      601 attcgcgggg atgacgcagg acctaagggc gggacgccct aaggtcttgc gtcacttgat
      661 cttggcttcc ttgaaatcga cgtgcttgcg cacgacggga tcgtacttgc ggaaggtcat
      721 cttctcggtg gtgttgcgcg gattcttctt ggtcacgtag aagaagccgg tgtcggcggt
      781 gctgaccagc ttgatcttga tggttgcggg cttcgccatg gcggcctctg ttcctgctct
      841 aatcgaaaaa ataaaagcgg cgtcgccgcc gctcagatgc cgcccgcctc tgccgcgacc
      901 ggcccgcttt gtcaagggcg ggccgggcga gggcgggcgg cctcgaggcc tattcgggca
      961 ggaagtcggg taccgacaga tagcgctcgc cggtatcgta gttgaagccg aggacgcggc
     1021 ttccggccgg cagctcgggc agcttctggg cgatggcggc gagcgtcgcg cccgagctga
     1081 tcccgaccag cagcccttcc tcgcgggcgg cgcggcgggc catttccttg gcgtcggcgg
     1141 ggtcgaccag gatgacgccg tcgatcgcgt ccacgtggag gttcttcggc acgaagccgg
     1201 cgccgatgcc ctggatgggg tgggggcccg gctggccgcc ggagatgacc ggcgacaagg
     1261 tcggctcgac cgcgaacacc ttgagccccg gccattcctg cttcagcact tccgcgcagg
     1321 cggtgatgtg gccgccggtg ccgacgccgg tgatgatgac gtcgatcggc gcgtcgcgga
     1381 aatcgcgcaa tatctcctgc gcggtggtgc ggacgtggac ggcgatattg gcctcgttct
     1441 cgaactgctg cggcatccag ctgcccggcg tcgccgcgac cagctcctgg gcgcgggcga
     1501 tggcggcctt catgccgccc tccttggggg tgaggtcgaa ctcggcgcca taggccagca
     1561 tcagccgccg ccgctccagg ctcatgcttt cgggcatgac caggatcagc cggtagccct
     1621 tgaccgcggc gaccatggcg aggccgatgc cggtattgcc gctggtcggc tcgacgatgg
     1681 tgccgccggg cttcagcgcg cccgaggcct cggcatcctc caccatggcg agcgcgatcc
     1741 ggtccttgat cgagccgccg gggttagcgc gctccgactt ggaccagact tcggcgtccc
     1801 caaacaggcg gttgatgcgg atgtgcggcg tgtcgccgat cgtctcgaga atgctggccg
     1861 ctttcatgtc gctgtctcct tggtagcggg aatgggattg gaacgggctg tcgcggctcg
     1921 gcgcgagcgg ctaatcttca cgggctccgg ccgcgccgcc ggcgccggga gagataggtg
     1981 acgccgatcc accatgactg cctcgacgcc ttcagcgccg ccgaatgcga cgcgatgatc
     2041 gccatcgccg ccgccgatgc gggcgaagcg gggccggtct ggggcggcgc gggctatgcg
     2101 ctcgatccgg cggtgcggag cgtgcgcacc gtgttccgcg ccaggagcga agagacgggc
     2161 tggatctacg accggctcga ccggctgttc ggcgaggcgg cggagcggct cggcgtcgcg
     2221 gtcgcgccgg tcagcgagga catccagatc atggcctatg gcgttggcaa ccacttccag
     2281 gtctggcaca gcgacgcggg gaccgacctg caggcgcggc gcatcctctc ggtctcggtc
     2341 gagctttcgg agcccggcga ccatgacggc ggcgacctcg agattgtgcc ggtggcgatc
     2401 ggccggcgga cgctgccgcg cggcgcggcg cggctgttcc tctcccgcgc gctccaccgg
     2461 gtgactccgg tgacgcgcgg gacccgccac tcgctggtga gctggaccgg cgcgcccgga
     2521 tgagcggctg aggctggcgg cgctcacggc acgccctttt cggctttggg gttcaagccg
     2581 gcgaccatcg ccggcagctc ggtctcggcg tcggccttgc ccggctggtc ggccagcact
     2641 gcctcgtcgg cggcgacgaa gcggtcggcg cggcgcaggc tcgggaaggc ccaggcgcaa
     2701 aggccggtga cgaccaccgc cagcgcgccg ccgccgacca ccgccaccac cggcccgaac
     2761 gcggccccgg cgaggccggc gcggaactcg ccaagctcgt tcgaagccga gatgaacagg
     2821 cccgacaccg ccgacacgcg gccgcgcatc gcgtcgggcg tgtggagctg gatcagcgag
     2881 gagcgcacat agacgctgat catgtcggcg gcgccgagca cgaacaggct ggcgagcgac
     2941 agccacagca cccggctttc gccgaacacg atggtggcga ggccgaacac ggcgacgcag
     3001 acgaacatct tgacaccaac ctgacgcctg agcggccggc gagcgaggat gagcgcggtg
     3061 agcgcggcgc cgaccgcggg cgcggcgcgg agcgcgccca gcccttcgga gccgacatgg
     3121 aggatgtcgc gcgcatagac gggaagcatc gcggtggcgc cgccgagcag gaccgcgaac
     3181 agatcgagcg tgatcgcgcc gagcacgatc ttgttgttgc gcacgtaagc cagcccttcg
     3241 atcaccgcgc gcagcgggtg cgaggcgccg gcattgccgc gcgggatcgg ccggatgacc
     3301 atgaaggcgc cgatcgcgag cgcgaacagc aatgccgaaa cctcgtaggg gagggcgatg
     3361 cgcagcgcgt aggcggcgcc gccgagcagg ggcccggcga ccgcgccggc ctgccaggcg
     3421 atcgagttga gcgcgatcgc gctgggcagg atttccttgg gcaccaaatt gggggcgaag
     3481 gaggccaaag ccgggccggc gaaggcgcgg ccgatgccga gcgccaccgc gaccgcgaac
     3541 aagggcgcga gcgagatcga atcggcccag acgaacgccg ccagcgccgc cgcgcagccg
     3601 agctcgagcg cgatgctggc gcggacgatc cagcgccggt cgatgcggtc ggcgatatag
     3661 ccggccacga gggtcagcag gaacaaaggc aggaactggg cgaggccgac catgccgagc
     3721 cagaagctcg cttccttgat gcccatggtg aggcgggcgg tgtcgtagac gtggatgccg
     3781 atgacgacga ccagcatggt gctgccgatg gtgctcgcca gccgcgccag ccagaaggca
     3841 cgatagtcgc gatagtggaa gggcgaggcg gccggcatca gctccgcccc ttagggaggc
     3901 gggcttcggg cggcaaccgg agcgatgcgg gatggcggaa tgtcgcggca agcggccgcg
     3961 gcggggaaga tcttgctccc tgagggcggg ttgggggcgg gaaaggtgac aaaggtggcg
     4021 caacgggggc ggagaattgc ggtgaaccga ggctcagccg acgcccggcc cgcgccgatc
     4081 ccagccctgc gcgtgcccgg cctgcagggc ggcgaggagc aggccgtcat tatagacgcg
     4141 gtcggtgccg catcggacgc cgcggtagaa atgcgggact tcgacgccgc ccgagcgctc
     4201 ggcggcggcg aggaaggcgt tgccgcggcc ttgctgctga gccgcggccc aggcggcggc
     4261 gaagccgctg tccgggccgg cgcgttcgag cagggcgtag gccgacttgc ggctcatgcc
     4321 gatgctcgcc gccgccgcct cgaccatgcc gatccgggcc agcgccgcga tgaagccgcg
     4381 ctgccgctcc ggcgtccacc cgtcgcggcg gcgggtggcg gaggggattg gctcgaaggt
     4441 gtagggatcg gaaggcgagt cgctcatttg gaagtgtatg acgcggaggc ggggctgtag
     4501 gaaagttctt tctttgttcc agacccgtcg gcggtggcgg ccgtcattcc agcgaaagct
     4561 ggaatctctt ttgcccttga tctctgcgcc gtgcgtccgg ctctgtcaaa gaaaagtgag
     4621 atcccagctt tcgctgggat gacggtgggg cggctcggcg ctcggccgcc tcagccgccc
     4681 tgaaaccgcc gcacccaggc ggcgaaggcg gtgaggaagc gctggaggta gcggcgggtg
     4741 gtctcgtcgg tgaggcggcc gtcggcgtcg aacttctcat gggctttgaa caccagcagc
     4801 tcgggctggg gcatgacgta ggaattggtg aattcgaacg cctggcggag ctggctctga
     4861 ccgcgggcgg tgccggtgat gccgggcgag gcgcccatca gggcgaccgg cttgccgccg
     4921 agcggcgcct ggccgggcgg gcgcgaggcc cagtcgaccg cattcttcat gacgccgggg
     4981 acgccatgat tatattccgg cgtcgccatc aagacgccgt cggccgcgct tatcgcctgc
     5041 ttgaagcggg ccacgccctc gggatcgccc gcggcctcga catcgccatt gtagagcggg
     5101 acgtcggcga ggtcgaagat cgagatcgcc atgccctcgg gtgcaagctc ctgcgccgcc
     5161 cgcagcagcg cgcgattgta ggaggcggcg cgcaggctgc cggcgatggc gaggatgttg
     5221 agcgggggcg gggcggatgc catcatgcga gctcccgcac ggccggcgcg cgctgcgcgg
     5281 gcggggtgat gacgatcgat tgcagcctaa ccatcatgcg ttctccggtg ccggcagggt
     5341 cacagctaag cggccgggca aattgggcat gctcaagggt gcaccagacg aaggcagcct
     5401 agagtgggct ggcggccgga ggcaatcacg cttcagctcg aaaaatagat actttccccc
     5461 atttttgctc tccctgtcgt ccctgattga aactgttaaa ggcgcggcgt atcccggcga
     5521 aggaacttca tcgcgctttt ctcgatcttg tataaccagc gcaagccttg aaagatgaac
     5581 cacagggata aaacgtaagc gatgctgtta aacggccaca gaaagctcaa tatatctccg
     5641 acataggcca caaaaacagc taacaagaag tacctgaggg cccttgcctg aacgatgcca
     5701 taggcaaatg gggcaaggga gtggctagaa tcaagtgcct gctcaaaaat ctgatcttcg
     5761 agccgtcgct tttcccgctg cgcccgactc aacgaaagcg cgtttcgaaa tacgtacacg
     5821 acgatgccga taattgaagc cccgacgatc gatgagctcg ccccgaccca aaaatctttt
     5881 ccgctggcaa tttgcatgag ttcaactgaa tccatagggt cctctcaacc gggattaaca
     5941 gatgattcca aacgcactgc ccaaagttcg attttgggcc ttaccaccct tcgagctgct
     6001 gctcctcata gacggtgtag atgatctggc gcatgctcgg tagaacccag tcaacccgat
     6061 aattaaccat cactctgtcg tgatcgcgag cgaggccgaa gccggaggcg atcgaccccg
     6121 cctcacggcc ggcaggcagc aggatcgaga tgtcctctgg ctcacccgtt ccgctaatgg
     6181 aatcgtcatt ttgctcaaat ctaaacctaa gggtctgacc gccctcgcta atgaccacct
     6241 cgctatcacc attcgagagg accacgccgc tcattatgat gcgctgaccg tccatcattg
     6301 ttgccattca aagctccctt gatctcactc taaacttacg aaactgtcca gcacgcgctt
     6361 gcggccggct tgctcgaaat cgatctcgag cttgttgcct tcgatctcgg cgactttgcc
     6421 gtagccgaat ttggagtgga agacgcgctg gccgagcgcg atgtcggtgc ggccggggtt
     6481 gccgaggctg acggcgctgg cgcgggcttc gatgacgcgc gggggggtgc gttcgaagcc
     6541 gccggtgacg gcgcggcgcc agccggggcc gcggccggtg ccgcgggcga cgtcggcgaa
     6601 cgggtcggca cgctcggacc agttggcgcg ccagagcgat tcgccgccgc tcatcgtggt
     6661 ctggtggtcc acatggggct cggggagctc ggcgacgaag cgggacggga tggacgaggt
     6721 ccattggccg tagatgcggc ggttggcggc gtgcatgatg aagcacttat ggcgcgccct
     6781 tgtgatcgcg acataagcga ggcggcgctc ttcctcgagg ctggcctggc cgccttcgtc
     6841 caaggagcgc tgcgagggga acacgccttc ctcccagccg gcgaggaaga cggtgccgaa
     6901 ttcgaggccc ttggcggcgt ggatggtcat gatcgtgacc ttctcctcgt cgcgggcggc
     6961 gtcattttcc atcaccagcg agacgtgttc gaggaaggcg ccgagggtgt cgtattcctc
     7021 catcgcgcgg gtgagctcgg tgaggttttc gagccggccg gcgctttcgg ccgagcggtc
     7081 ggcctggagc atggcggtgt agccggattc gtcgaggacg aggcgggcga gctcggcgtg
     7141 ggggaggcct ccggcgggag aggcccctcc cgaagaggcc cctccatccg aaaaggcccc
     7201 tccgcccgaa gaggcccctc caccagcctg cggctggtcc ccctccccgg caaaggccgg
     7261 ggaggagttg gagagcatcc tgcgccatct tgcgaagtcg gcgacgaggt tggcgagcga
     7321 cttgcgggcg gcggcggtga gctcgtcggt gtcgaggatc tgggcggcgg cggagaagag
     7381 cgagacgccg gcggcgcggg cgaagccgtg caccttggcc accgccttgt cgccgaggcc
     7441 gcgcttgggg gtgttgacga tgcgctcgaa ggcgaggtcg tcggagggct gggcgatgat
     7501 gcgcagatag gcgagggcgt cgcggatctc ggcccgctcg tagaatcgga agccgccgac
     7561 gatgcggtag ggca
//
LOCUS       DASVRK010000178         7963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_531306, whole genome shotgun sequence.
ACCESSION   DASVRK010000178 DASVRK010000000
VERSION     DASVRK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531306"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF589_07015"
     CDS             complement(<1..244)
                     /locus_tag="VF589_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat biosynthesis protein F"
                     /protein_id="HEX8257366.1"
                     /translation="MTAIPRILVLAGRRAAALDSLAAAAGVTHKCLVPVAGEAMLGRV
                     LRIVEAAFPDAPVFVSVEDFATVAAEPTVARLHAGGR"
     gene            complement(311..1837)
                     /gene="rpoN"
                     /locus_tag="VF589_07020"
     CDS             complement(311..1837)
                     /gene="rpoN"
                     /locus_tag="VF589_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX8257367.1"
                     /translation="MALGPRLDLRQSQSLVMTPQLQQAIRLLALSNLEVEGFIAEELE
                     KNPLLDTGAAAASGEEEAAPEAAGPELLHDTGAEGLDRLIAGGDAVADAPLDIDYDSE
                     AFHQDSAADGMRGLDGGLGLDGIAGGSGAASGEGPHYDSFASEPVSLHDHLLGQAGEC
                     LSGKDFALAAQIIEQIDETGYFHASLLDLAQRLGVALHEAERVLAMVQTFDPAGVGAR
                     SLAECLALQAKDANRYDPAMARLIDNLDYLAKGNIGALKRICGVDDEDMVDMVRELRA
                     YDPKPGCRFTQGGGASAVVPDVFVARRGQGWAIELNGATLPKLLVNRSYYNELSTGAQ
                     DRQSKAWLSECLTSANWLMKALDQRARTIVKVATEIVKQQDGFFRNGVAHLRPLTLRT
                     VADAIGMHESTVSRVTSNKYLSCDRGLYELKFFFTSGIQSSDGGDAVSAEAVKSHIKA
                     LIAAEGLRQILSDDQLVDLLRARGFDIARRTVAKYREACGIGSSVQRRRQKAIGSHAA
                     "
     gene            complement(1848..2627)
                     /gene="lptB"
                     /locus_tag="VF589_07025"
     CDS             complement(1848..2627)
                     /gene="lptB"
                     /locus_tag="VF589_07025"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX8257368.1"
                     /translation="MNDTALIERPREDLPPGQVARGLSVVSIAKTYDRKTVLADVSLS
                     VGRGEVVGLLGPNGAGKTTCFYSIMGLVKPDSGRILLDGEDITGLPMYRRAILGLGYL
                     PQETSIFRGLTVAQNIMAVLEVSEPKRQVREERLEQLLGEFHLERLRDAPAMALSGGE
                     RRRCEIARALAADPTIMLLDEPFAGIDPISISDIRGLVTELKQRDIGVLITDHNVRET
                     LDIVDRAAIIYDGQVLFAGSPQALVANEDVRRLYLGESFAL"
     gene            complement(2772..3314)
                     /locus_tag="VF589_07030"
     CDS             complement(2772..3314)
                     /locus_tag="VF589_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX8257369.1"
                     /translation="MTKRATIWVAAILAAAAIGGPAAGQGQTSALQGHNTNAPVDVAA
                     DRIEVQDRADRAIFSGNVVARQANLTLNAGRITVAYSNTGGIEIQRIDASGGVTMRSP
                     SETARGQFAIYDLNSRIITMVGGVTLTRGASHVQGGRLVLDLNSGRAVMDGSGASPAG
                     TAQGEAGRVTGTFTVPQRGE"
     gene            complement(3320..3958)
                     /gene="lptC"
                     /locus_tag="VF589_07035"
     CDS             complement(3320..3958)
                     /gene="lptC"
                     /locus_tag="VF589_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX8257370.1"
                     /translation="MSELAERERVRRQGWAAPGGAHDYLVRLLKLALPIGIGVMMAYL
                     ALAPLTKGRDISFILDKTKVAVAKERMRVQTARYQGQDNLGRQFVLEARSAVQATSSE
                     PIVDVLGMNARLALENGPATLAADKGRYNLESERVQVIGPILFTAADGYRLQTRDVAV
                     DLNTRTMASNGRVDGQMPLGRFSADRMVADLPGREVTLTGRARLYIVQGGLR"
     gene            complement(3951..4568)
                     /locus_tag="VF589_07040"
     CDS             complement(3951..4568)
                     /locus_tag="VF589_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8257371.1"
                     /translation="MTVCFHEEDLPEGVLAPGAVAVDTETMGLQLGRDRLCVVQISDG
                     RGDEHLVRFRAGSDYAAPNLKAVLTDPERLKLYHFARFDLASIYAYLGVVAAPVYCTK
                     TASRLIRTYTDRHGLKELVKELLGHEISKQQQTSDWGAPELSDAQKDYAASDVRYLHQ
                     LKEKLDERLARERRTELAQACFDFLPHRALLDLAGWAEVDIFAHV"
     gene            4704..5636
                     /locus_tag="VF589_07045"
     CDS             4704..5636
                     /locus_tag="VF589_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEX8257372.1"
                     /translation="MPSGLAALLDDVAAIAKLAAASVDDIGAAAGRAGVKAAGVVVDD
                     TAVTPRYVTGFTPDRELPIIARIATGSIKNKLLIILPAALALSALLPWAITPLLMLGG
                     AYLCFEGAEKVWEAVSGHGHSLEETAAELGSESHEREMVAGAVRTDFILSAEIMAIAL
                     AEVASEPFATRAAILAVVAIVITVGVYGVVGLIVKMDDIGLHLAARRDGASRAVGRGL
                     VYAMPRLMAALSVIGIAAMIWVGGGIIVHGLEAFGFAGIAHVIHDTAEAAGHGVPALA
                     GTVEWTVGAAGAGVSGLVVGAAIVAVHHAVARKH"
     gene            5804..6673
                     /locus_tag="VF589_07050"
     CDS             5804..6673
                     /locus_tag="VF589_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8257373.1"
                     /translation="MSFDRGRRGDRGGRGRDKRDGFGEDSYSAGYGQDRGGFGGGDRF
                     GGGGGGGGGDRFGGGGGDRFGGGGGGGDRFGGGGGDRFGGGGGGGGFRGGGGGGFGGP
                     RGGGMPPQVVGEGKGIVKFFNSQKGFGFIVRDDGGEDVFVHISAVEQAGLTGLAEGQP
                     LEFTLVDRGGRISATDLKIEGEPMAVQERAPRDDRAGPSAGGFGGGGGDRGGPQRQLT
                     GEKASGTVKFFNAMKGFGFISRDDGQPDAFVHISAVERAGMVSLNEGDRLEFELEVDR
                     RGKTAAVNLAPIQ"
     gene            6751..7107
                     /locus_tag="VF589_07055"
     CDS             6751..7107
                     /locus_tag="VF589_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257374.1"
                     /translation="MRGSKFLLPLAPLIVAAAPAEDPNLADAECLVVLGQLGASEDAA
                     TRNAGMIGAQYYLGRLDGRAPAGDLRALLKRAAETLKPADLQPILTRCGTVLQQRGQA
                     LQAIGQALAKEAPAAK"
     gene            complement(7118..7375)
                     /locus_tag="VF589_07060"
     CDS             complement(7118..7375)
                     /locus_tag="VF589_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257375.1"
                     /translation="MLRPIIDRAGESFPHNPVARGYHVVYRENAVNHCPGCGRTHWYV
                     GRFSAECGFCSTALPLAEATARGSGGIFHGHRSVWQESDAA"
     gene            complement(7547..>7963)
                     /locus_tag="VF589_07065"
     CDS             complement(7547..>7963)
                     /locus_tag="VF589_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HEX8257376.1"
                     /translation="YIHGALQRLCELYRELGVELGELRPATGAAKEELKGRIVLAPPG
                     ALNDRWSRRLPDPITAMASGWMRVRQRARQRNVELPLIVSDHADWDELTRTIAELAPS
                     EVWVTHGREDALIHWCAMRQIKARELNLVGYEDEDD"
BASE COUNT         1166 a         2679 c         2862 g         1256 t
ORIGIN      
        1 gcctgccgcc cgcgtggagc cgggccaccg tcggctcggc ggcgacggtc gcgaaatcct
       61 cgaccgagac gaacacaggc gcgtcgggaa aggccgcctc gacgatcctc agcacgcgcc
      121 ccagcatcgc ctcgcccgcc accggcacca ggcatttgtg agtcaccccg gcggcggcgg
      181 cgagcgaatc gagggccgcg gcgcggcggc cggcgagcac caggatgcgc ggaatcgcag
      241 tcatgcgccc tcatgcacga tttgcgcgac aggggaagcg cgcgacgtgg cacgccgccg
      301 ggccggagcc tcaggcggcg tggctgccga tcgctttctg gcgccgccgc tggaccgacg
      361 agccgatccc gcacgcctcg cgatatttgg cgacggtgcg gcgggcgatg tcgaagcccc
      421 tggcgcgaag caggtcgacc agctgatcgt cggacaatat ctgccgcaag ccctcggcgg
      481 cgatcagcgc cttgatatgg cttttgaccg cctcggccga gaccgcgtcg cccccgtcgg
      541 agctctggat gccgctggtg aagaagaatt tgagttcgta caggccgcgg tcgcaggaca
      601 gatatttgtt cgacgtgacc cggctcacgg tcgattcgtg catgccgatc gcgtcggcga
      661 cggtgcgcag ggtcagcggc cgcaaatgcg cgacgccgtt gcggaagaag ccgtcctgct
      721 gcttcacgat ctcggtcgcc accttgacga tggtgcgcgc cctctggtcg agcgccttca
      781 tcagccagtt ggcgctggtg aggcattccg acagccaggc cttggactgc cggtcctgcg
      841 ccccggtcga cagttcgtta taatagctgc ggttgacgag cagcttcggc agggtggcgc
      901 cgttgagctc gatcgcccag ccctggccgc ggcgcgcgac gaacacgtcc ggcacgaccg
      961 ccgaggcccc gccgccctgg gtgaagcggc agccgggctt ggggtcatag gccctgagct
     1021 cgcggaccat gtcgaccatg tcctcgtcgt cgacgccgca gatccgcttc agcgcgccga
     1081 tattgccctt ggcgagatag tcgagattgt cgatcagccg cgccatcgcc ggatcgtagc
     1141 gattggcgtc cttggcctgg agggcgaggc attcggcgag cgaccgggcg ccgacgccgg
     1201 ccggatcgaa agtctggacc atcgccagca cccgctcggc ctcgtgcagc gccacgccca
     1261 gccgctgcgc cagatcgagc agggaagcat ggaaataacc ggtctcgtcg atctgctcga
     1321 tgatctgcgc ggccagggcg aagtccttgc ccgacaggca ttcgccggcc tggccgagca
     1381 gatggtcgtg gagcgacacc ggctcgctgg cgaagctgtc ataatggggc ccctcgcccg
     1441 atgccgcgcc cgagccgccg gcaatgccgt cgagccccag cccgccgtcg aggccccgca
     1501 tgccgtcggc ggcgctatcc tggtggaagg cctcgctgtc atagtcgatg tcgagcggcg
     1561 cgtcggccac cgcgtcgccg cccgcgatca gccggtcgag gccctcggcg ccggtgtcgt
     1621 ggagcagctc cgggccggct gcctcgggcg ccgcttcctc ctcgccgctg gcggccgcag
     1681 cgccggtatc gagcaggggg ttcttttcga gctcctcggc gatgaagcct tcgacctcga
     1741 ggttcgacag ggccagcagc cggatcgcct gctggagctg cggcgtcatt accagcgact
     1801 ggctctgacg caggtccagg cgggggccga gcgccatggc tatcccgtca cagcgcgaag
     1861 ctttcgccga gatacagccg ccgtacgtcc tcgttggcga ccagcgcctg cgggctgccg
     1921 gcgaacagca cctggccgtc gtaaatgatc gccgcgcggt cgacgatgtc gagcgtctcg
     1981 cggacattgt gatcggtgat gaggacgccg atgtcgcgct gcttgagttc ggtcacgagc
     2041 ccgcgaatgt cggagatcga gatcgggtcg atgccggcga acggctcgtc gagcagcatg
     2101 atggtcgggt ccgccgccag cgcccgcgcg atctcgcagc gccgccgctc accgcccgag
     2161 agcgccatcg ccggggcgtc gcgaaggcgc tcgaggtgga actcgccgag caattgttcg
     2221 agccgctcct cgcgcacctg gcgcttgggc tcggacactt cgagcaccgc catgatgttc
     2281 tgcgccaccg tcaggccgcg gaagatcgag gtctcctgcg gcagatagcc gaggccgagg
     2341 atcgcgcgcc ggtacatggg gaggccggta atgtcctcgc cgtcgagcag gatgcggccg
     2401 ctgtcgggct tcaccagccc catgatcgaa tagaagcagg tggtcttgcc ggcgccgttg
     2461 gggccgagca gcccgaccac ttcgccgcgc ccgacgctga gcgacacgtc ggcgagcacc
     2521 gtcttgcggt cgtaggtctt ggcgatcgac accaccgaca ggccgcgcgc gacctgcccc
     2581 ggcgggagat cctcccgcgg ccgttcaatg agggcagtgt cgttcatgcg cgctccgctg
     2641 gcttgctccg gggtttaggc gaagcaccgt gacgcctcaa ctggcaagct tcgtgcatgg
     2701 cgagtttccg ccctttgagc ggcgttagcg ccacactttt gcagccgccg gacgcggcca
     2761 tgatcaatgg cttactcgcc gcgctggggc accgtgaacg tgccggtgac gcggcctgcc
     2821 tcgccttgcg cggtgccggc cggcgatgcg ccgctgccgt ccatcaccgc ccggccgcta
     2881 ttgaggtcga gcaccagccg gccgccctgg acgtgcgaag cgccgcgggt cagcgtcacc
     2941 ccgccgacca tggtgatgat gcggctgttg aggtcgtaaa tcgcgaactg gccgcgcgcc
     3001 gtctccgacg ggctgcgcat ggtgacgccg ccgctggcgt cgatgcgctg gatctcgatc
     3061 ccgccggtgt tggaataagc gaccgtgatc cggccggcgt tgagcgtgag gttggcctgg
     3121 cgcgcgacga cgttgccgga gaagatcgcg cggtcggcgc ggtcctgcac ctcgatgcgg
     3181 tcggcggcga cgtcgaccgg cgcgttggtg ttgtggccct gcagcgccga ggtctgcccc
     3241 tgccccgcgg ccgggccgcc gatcgcggct gcggcgagga tcgcggctac ccagattgtc
     3301 gcccgcttgg tcatcccgct catctgaggc cgccttgcac gatatacaag cgcgcgcgtc
     3361 cggtcagcgt cacctcgcgg ccgggcaggt cggcgaccat gcgatcggcg gagaagcggc
     3421 cgagcggcat ctgcccgtcg acgcggccgt tgctggccat tgtccgggta ttgaggtcga
     3481 ccgccacgtc gcgcgtctgc agccggtagc cgtcggcggc ggtgaacagg atcgggccga
     3541 tcacctgcac gcgctcgctt tcgagattgt agcggccctt gtcggcggcg agcgtggccg
     3601 ggccgttttc gagggcgagc cgggcgttca tgccgagcac gtcgacgatc ggctcgctcg
     3661 aggtcgcctg caccgccgag cgcgcctcga ggacgaactg acggccgaga ttgtcctgcc
     3721 cctggtagcg cgccgtctgg acccgcatcc gctccttggc gaccgcgacc ttggtcttgt
     3781 cgagaatgaa gctgatgtcg cggcccttgg tcagcggcgc cagcgccaga taggccatca
     3841 tcacgccgat cccgatcggc agcgccagct tcaagaggcg gacgaggtaa tcgtgggcgc
     3901 cgccgggcgc ggcccagccc tgccggcgca cccgttcgcg ttcggccagc tcagacatgc
     3961 gcgaagatgt ccacctcggc ccagccggcg agatcgagca gggcgcggtg gggcaggaaa
     4021 tcgaagcagg cctgggcgag ctcggtccgc cgctcgcgcg ccagccgctc gtcgagcttc
     4081 tccttcaatt gatggaggta gcgcacgtcg gacgccgcat aatccttctg cgcgtcgctg
     4141 agctccggcg cgccccagtc ggaagtctgc tgctgcttcg atatttcgtg acccagcaat
     4201 tccttgacca attccttgag cccgtgccgg tcggtatagg tccggatcag gcgcgaggcg
     4261 gtcttggtgc aatagaccgg cgcggcgacc accccgagat aggcgtagat ggaggcgagg
     4321 tcgaagcgcg cgaagtgata gagtttgagc cgctccggat cggtgagcac cgccttgagg
     4381 ttgggcgcgg catagtcgct gccggcgcgg aagcggacga gatgctcatc cccccgcccg
     4441 tcggagatct ggaccacgca cagccggtcg cggccgagtt gcaggcccat ggtctcggtg
     4501 tcgacggcga ccgcgcccgg cgcgagcacc ccctcgggca ggtcttcttc gtgaaagcat
     4561 acggtcatag gggcatgccc ttaaggcaaa gcaccgggcg gctcaatgat gcgccaccca
     4621 cgcggctgca ccggtgccgg gcttgatcgc gccgccgcga cttgccagag gggcaccccc
     4681 gcagttcgag gaagccacca cccatgccca gcggtctcgc cgccttgctc gacgacgtcg
     4741 ccgccatcgc caagctggcc gcggcatcgg tggacgatat cggcgccgcg gccgggcgcg
     4801 ccggggtgaa ggcggcgggc gtggtggtcg acgataccgc ggtcacgccg cgctacgtca
     4861 ccggcttcac gcccgaccgc gagctgccga tcatcgcgcg gatcgccacc ggatcgatca
     4921 agaacaagct gctcatcatc ctgccggcgg cgctggcgct gagcgcgctg ctgccctggg
     4981 cgatcacgcc gctgctgatg ctgggcggcg cctatctgtg cttcgagggc gccgaaaagg
     5041 tgtgggaagc cgtttccggc cacggccaca gcctcgagga gaccgcggcc gagctcggca
     5101 gcgagagcca cgagcgcgag atggtcgccg gcgcggtccg caccgacttc atcctgtcgg
     5161 ccgagatcat ggcgatcgcg ctggccgaag tcgccagcga gccgttcgcg acgcgggccg
     5221 cgatcctcgc ggtggtcgcg atcgtgatca cggtcggcgt ctacggcgtc gtcggcctga
     5281 tcgtgaagat ggacgatatc gggctgcatc tggcggcgcg gcgcgacggc gcgtcccggg
     5341 ccgtggggcg cgggctggtc tatgccatgc cgcggctgat ggcggctttg tcggtgatcg
     5401 gcatcgcggc gatgatctgg gtcggcgggg gcatcatcgt ccatgggctc gaggcgttcg
     5461 gtttcgccgg gatcgcgcat gtcatccacg ataccgccga ggctgccggg cacggcgtgc
     5521 cggcgctggc cggaaccgtg gaatggacgg tgggcgccgc cggcgcgggc gtgagcgggc
     5581 tcgtcgtcgg ggccgcgatc gtggccgtcc accatgcggt cgcgcgcaag cattgacggc
     5641 gggttcgccc cggccgcgcg gactccgttt ttcgttcgcg gcgatgacca aattgcgtta
     5701 tacgactcgg cggtgcgccg atttcgcgcc aaaggccgtt tgcgcgtttt gcccagcgcg
     5761 gtggttctaa cagggtttcg ggcgcgggaa agactagaac ggcatgagtt tcgacagagg
     5821 gcgtcgcggt gatcgcggcg ggcgcggcag agataagcgc gacggtttcg gcgaggacag
     5881 ttacagcgcc ggttatggcc aggatcgggg cggcttcggc ggcggcgatc gcttcggcgg
     5941 cggcggcggt ggcggcggcg gcgatcgctt cggcggtggt ggcggcgacc gcttcggcgg
     6001 cggcggcggc ggcggcgacc gtttcggcgg cggtggcggc gatcgcttcg gcggcggcgg
     6061 cgggggcggc ggcttccggg gcggcggtgg cggcggcttc ggcggcccgc gcggcggcgg
     6121 catgcccccg caggtcgtcg gcgagggcaa aggcatcgtc aaattcttca actcgcagaa
     6181 gggcttcggc ttcatcgtcc gcgacgatgg cggcgaggat gtgttcgtgc acatctccgc
     6241 ggtcgagcag gccggcctca ccgggctggc cgagggtcag ccgctcgaat tcaccctggt
     6301 cgaccggggc gggcgcattt ccgcgaccga cctcaagatc gagggcgagc cgatggcggt
     6361 gcaggagcgc gcgccgcgcg acgatcgcgc cggccccagc gccggcggct tcggcggcgg
     6421 cggcggcgac cgcggcgggc cgcagcgcca gctgaccggc gaaaaggctt cgggcacggt
     6481 caagttcttc aacgccatga agggcttcgg cttcatcagc cgcgacgacg gccagccgga
     6541 cgcgttcgtc catatctcgg cggtggagcg ggccggcatg gtcagcctca acgagggcga
     6601 ccggctcgag ttcgagctcg aagtcgaccg ccgcggcaag acggcggcgg tgaacctcgc
     6661 gccgatccag taagcgcttc agcttcgcga aaggcccggc cgcgcaggag cgcgccgggc
     6721 ctttttcgta aagcagggct ggagatgatg atgcgcggtt cgaaattcct acttccccta
     6781 gccccgctca tcgtcgcggc ggcgccggcc gaggatccca accttgccga cgcagaatgc
     6841 ctcgtcgtgc tggggcagct cggcgcgtcg gaggatgccg cgactcgcaa tgcggggatg
     6901 atcggagcgc aatattatct ggggcggctc gacggccgtg ccccggccgg cgacctccgc
     6961 gcgctgctga agcgggccgc ggagacgctc aagccggccg acctgcagcc aatcctgacg
     7021 cgctgcggca cggtgcttca gcagcgcggg caggcgctcc aggcgatcgg acaggcgctc
     7081 gccaaggaag cgcccgccgc caaatagcgt gcgtcgctta ggcggcgtcg ctctcctgcc
     7141 agaccgagcg gtggccgtgg aagatgccgc cgctgccgcg cgcggtagct tcggcgagcg
     7201 gcaaagcggt gctgcaaaag ccgcattcgg ccgaaaagcg gccgacatac caatgcgtac
     7261 ggccgcagcc ggggcagtgg ttcactgcat tttcgcggta gacgacatga tagccgcggg
     7321 cgaccggatt gtgcggaaag ctctccccgg cgcggtcgat gatcggtctc agcacggcga
     7381 tattcccctg ataagcaact cccgaaccaa caaggttgcg ggtggatttt gctatgatcc
     7441 aaagctaaat cactgtcacc acgccaaatt ccgccgttcc gaaaagggac gggatggttc
     7501 gcgccggtta acggcgcttg gccgccgggt cgccggccgc cgccggtcag tcgtcctcgt
     7561 cctcatagcc gaccaggttg agctcgcgcg ccttgatctg gcgcatcgcg caccagtgga
     7621 tgagggcgtc ctcgcggccg tgggtgaccc agacctcgct cggcgccagc tcggcgatgg
     7681 tgcgggtgag ctcgtcccaa tcggcatggt cggagacgat cagcggcagc tcgacgttgc
     7741 gctggcgcgc gcgctggcgc acccgcatcc agcccgaggc catggccgtg atcggatcgg
     7801 gcagccgccg cgaccagcgg tcgttgagcg cgccaggcgg ggcgagcacg atccggccct
     7861 tcaattcctc cttcgccgct ccggtggcgg ggcgaagctc gccaagctcg acgccaagct
     7921 cccggtaaag ctcgcacagc cgctgcagcg cgccgtgaat gta
//
LOCUS       DASVRK010000179         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_221030, whole genome shotgun sequence.
ACCESSION   DASVRK010000179 DASVRK010000000
VERSION     DASVRK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221030"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF589_07070"
     CDS             complement(<1..481)
                     /locus_tag="VF589_07070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8257377.1"
                     /translation="MKAAGWALAALLAASTSACGDSPKDYAPEVTDADRAQAAEQHPQ
                     LLAEFGGAYDGPQARYLAALGGKLAAAAGLDGQCTFTLVNTDVVNAFAVPGCYIYITR
                     GLFGIVNSEAELASVLGHEVGHIVAAHSRRQQRRSLLTGLGALAVGVLTGSERLAQVA
                     "
     gene            645..836
                     /locus_tag="VF589_07075"
     CDS             645..836
                     /locus_tag="VF589_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257378.1"
                     /translation="MTYYRLYALRRPDGPLIGVDEIAAEDDVQAVRSAAARAGETPME
                     LWCGKRKVKSFPALEQQLA"
     gene            complement(952..1611)
                     /locus_tag="VF589_07080"
     CDS             complement(952..1611)
                     /locus_tag="VF589_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEX8257379.1"
                     /translation="MIPPNEMTLDELRGALAPLLPAHAVFDGWSDEALANAAAELGIP
                     AGRARLAFPDGPVQMIDGWFDAIDLAMAKAWPLDRIARLKIRERIRDLVLYRIEVINP
                     HKEALRRALAILADPRNGLTGPRLAWRAADRMWRIAGDVSTDFNHYSKRTILAGLYSA
                     TMLVYLDDESEGLATTRGFLDRRIDDVMSFEKFKAQWRGGRSRLPSLSRFLGRLRYPA
                     T"
     gene            complement(1608..2540)
                     /locus_tag="VF589_07085"
     CDS             complement(1608..2540)
                     /locus_tag="VF589_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8257380.1"
                     /translation="MHQPKSPGAPAGARYAFAALIVSNLFLALGPWMVRLADVGPVAA
                     AFWRLALAIPLLLLLARRQIRERVHPSWGIAAMIVVGGLFFAADLAAWHYGIVRTKLA
                     NATLFGNTSSFIFALYGFVVARTLPRRLQAAALALAALGAALLLGSSYELSAEHFEGD
                     LFAILAGIFYFFYLVLVDRARQTLAPLPVLALATIAGTLPLLGFALLLGERVMPGDWT
                     PLILLSLGSQVIGQGLLVYAMGHLSPVVVGLGLLTQPALTALVGWLAYDERLSLTDAA
                     GALLICAALVLIRLPARLASAGGEPHLEGDRRAP"
     gene            2593..3675
                     /locus_tag="VF589_07090"
     CDS             2593..3675
                     /locus_tag="VF589_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX8257381.1"
                     /translation="MPSLFDPISLGALICPNRILMAPLTRGRATREHVPTAIMKDYYV
                     QRARAGLIITEATGMSRQGLGWPYAPGVWTAEQMEAWKPITAAVHEAGGRIFCQLWHM
                     GRLVHSSFLGGDQPVSASPTTAPHKAHTYDGRQPYDEARALAADEIPKLLGDYARAAG
                     YAIAAGFDGVQLHAANGYLIDQFLRDATNHRSDDYGGPVENRIRLLREATQAIADIIG
                     PERTAVRLSPNGDSQGVNDSDPEPLFVAAAAALAEIGIAFLELREPGRGGTFGQGEVD
                     PVAPAIRKVFTAPLVLNSDYTHGSAEAALKAGEADAISFGRPFISNPDLVDRLARGAE
                     LSPDDRRTWYSQGADGYVDYPAMAAG"
BASE COUNT          644 a         1290 c         1349 g          539 t
ORIGIN      
        1 ccgcgacctg cgcaagccgc tccgagccgg tgagcactcc caccgcaagc gcgccgagcc
       61 cggtgagtag actacgccgc tgctgccgcc gcgaatgggc ggcgacgatg tgcccgacct
      121 cgtggccaag caccgaggcc agctccgcct cgctgttgac gatcccgaac aggccgcggg
      181 tgatgtagat atagcagccg gggaccgcga aggcgttgac cacgtcggta ttgaccaaag
      241 tgaaggtgca ttgcccgtcg agcccggcgg cggcggcaag cttgccgccg agggcggcga
      301 gatagcgcgc ctgcggcccg tcataggcgc ccccgaactc cgccagcagc tggggatgct
      361 gctcggcggc ctgggcccgg tcggcgtcgg tgacttcagg ggcgtagtcc ttgggcgaat
      421 ctccgcaggc cgacgttgaa gccgccagca gggccgccag cgcccatccg gcggctttca
      481 tgctcctccc gtccatcgca gcagctacgc acaagggctc tgcttggttg cgggtaagaa
      541 tcgactttga tttcgtcttt tgcgggcatg taaatctaag tgactcccac tccacggaat
      601 catggtaaac tgccgttaga ctcgagtcgc ggagggaaca ggccatgact tattacaggc
      661 tctacgcctt gcggcgcccg gacggcccgc tgatcggggt cgacgagatc gccgccgagg
      721 acgacgtcca ggcggtgcgg tcggcggcgg cgcgggcggg ggagaccccg atggaattgt
      781 ggtgcgggaa gcgcaaggtg aagagcttcc cggcgctcga gcaacagctc gcctgaggcg
      841 agcgagaaca aaagacccga ttgctccccg gttgcccacc ccgcagcaat ccacctacgc
      901 cccggccatc cggggcgttt tgctgtgcgg ccctgaagat cagcggcgag atcaggtggc
      961 gggatagcgc aaccggccga ggaagcggct gaggctcggg aggcggctgc ggccgccgcg
     1021 ccactgcgcc ttgaacttct cgaagctcat gacgtcgtcg atgcggcggt cgaggaagcc
     1081 gcgggtcgtc gccagcccct cgctctcgtc gtcgagatag accagcatgg tggcgctgta
     1141 gaggccggcg aggatggtcc gcttcgaata atggttgaag tcggtcgaga cgtcgccggc
     1201 gatccgccac atgcgatcgg cggcgcgcca ggcgaggcgc gggccggtaa gtccgttgcg
     1261 gggatcggcg aggatggcga gcgcgcgtcg cagcgcctcc ttatgcgggt tgatcacttc
     1321 gatccgatag aggaccaggt cgcggatgcg ctcgcggatc ttgagcctgg cgatgcggtc
     1381 gagcggccag gccttggcca tggcgaggtc gatggcgtcg aaccagccgt cgatcatctg
     1441 cacgggcccg tcggggaagg cgagccgggc gcggccggcg gggattccga gctcggcggc
     1501 ggcattggcc agcgcctcgt cgctccagcc gtcgaacacg gcgtgggccg ggagcagcgg
     1561 cgccagcgcg ccgcgaagct cgtcgagcgt catctcattg ggcgggatca tggggctctc
     1621 cgatccccct caagatgagg ctccccgccc gccgatgcaa gccgcgcagg gagccggatc
     1681 agcacgaggg cggcgcagat cagcagcgcg ccggcggcgt cggtcaggct cagccgctcg
     1741 tcataggcga gccagccgac gagggccgtc agcgccggct gggttaggag gccgaggccg
     1801 accaccaccg ggctcagatg gcccatcgca tagacgagca gcccctggcc gatcacctgg
     1861 ctgccgagcg agagcaggat caaaggcgtc cagtcgccgg gcatgacccg ctcgccgagc
     1921 agcaaggcga agccgagcag cggcaaggtg ccggcgatgg tggcgagcgc gagcaccggc
     1981 agcggcgcca gcgtctgccg cgcccgatcg accaaaacca ggtagaagaa gtagaagatg
     2041 ccggccagga tcgcgaacaa atcgccttcg aaatgctccg ccgaaagctc gtagctgctg
     2101 ccgagcagca aagccgcacc cagcgccgcg agcgccagcg ccgccgcctg caggcgccgc
     2161 ggcagcgtgc gggcgacgac gaaaccgtaa agcgcgaaga tgaagctcga cgtgttgccg
     2221 aacaaagtgg cgttggcgag cttggtgcgg acgatcccgt aatgccatgc ggcgaggtcg
     2281 gccgcgaaga acaggccgcc gacgacgatc atcgcggcga tgccccagct gggatggacc
     2341 cgctcgcgga tctggcgcct cgccagcagg agcagcagcg ggatcgccag cgccagccgc
     2401 cagaaggccg cggcgaccgg gccgacatcg gccagcctca ccatccacgg tcccaaagcg
     2461 aggaacaggt tcgacacgat cagggccgcg aaggcgtagc gggcgccggc cggagccccg
     2521 ggtgatttgg gttggtgcat ggcggctgcc gagccgctag ggctccgccc tccccgccgt
     2581 caaggagtgc cgatgccgtc tttgttcgat ccgatcagcc tgggcgcgct catctgcccc
     2641 aaccgcatcc tcatggcgcc gcttacgcgc gggcgggcga cgcgcgagca tgtgccgacc
     2701 gcgatcatga aagactatta tgtccagcgc gcgagggccg gcctgatcat caccgaagcg
     2761 accggcatga gccgccaggg gctcggctgg ccctatgcgc ccggcgtctg gacggccgag
     2821 cagatggagg cgtggaagcc gatcaccgcg gcggtgcacg aagccggcgg gcgcatcttc
     2881 tgccagctct ggcatatggg ccggctggtc cattcgagct tcctcggcgg cgaccagcca
     2941 gtctcggcct cgccgacgac ggcgccgcac aaggcgcaca cctatgacgg gcggcagcct
     3001 tatgacgagg cgcgggcgct ggcggcggac gagataccga agctgctcgg cgattatgcc
     3061 cgcgccgccg gctacgcgat cgcggcgggg ttcgacggcg tccagctcca cgccgccaat
     3121 ggctatctga tcgaccagtt cctgcgcgac gccaccaacc atcggagcga cgattatggc
     3181 gggccggtgg agaaccggat ccggctgctg cgcgaggcga cgcaggcgat cgccgacatc
     3241 atcggcccgg agcggacggc ggtgcggctg tcgccgaacg gggattcgca gggcgtcaac
     3301 gacagtgacc ccgaacccct gttcgtcgcc gccgcggcgg cgctggccga gatcggcatc
     3361 gccttcctgg agcttcgcga gcccggccgc ggcggaacgt tcggccaggg cgaggtcgat
     3421 ccggtcgcgc ccgccatccg caaggtcttc accgcgcctt tggtgctgaa ttccgactac
     3481 acccacggct ccgccgaggc ggccctgaag gccggcgagg ccgacgcgat cagcttcggc
     3541 cggccgttca tctccaaccc cgatctggtc gaccggctgg cgcgcggcgc cgagctctcc
     3601 cccgacgacc gccgcacctg gtacagccag ggcgccgacg gctatgtcga ttatcccgcg
     3661 atggcggcgg gctgaggggt tcccgctggc gcgaggagca gagccctctt cgtcattcca
     3721 gcgtaagctg gaatctccct tcttgaaggc agtgagattc cagcttgcgc tggaatgacg
     3781 ggctacccct ctctctcccc cctaaccccc gaaccgctcc cg
//
LOCUS       DASVRK010000180         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_95628, whole genome shotgun sequence.
ACCESSION   DASVRK010000180 DASVRK010000000
VERSION     DASVRK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95628"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF589_07095"
     CDS             complement(<1..749)
                     /locus_tag="VF589_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8257382.1"
                     /translation="MLKPEERRNRPALALHVPEPEARPGDEVDFSHIAIPAAGSVPRP
                     DVAASPHDMRDLAYSLVRVLDEEGKAVGPWDPRLDADTLRRMLRDMAMVRVYDDRMYR
                     AQRQGKTSFYMKSTGEEAIAVAAAHALDRDDMCFPTYRQQGLLVARDYPLVQMMCQVY
                     SNRGDPLKGRQLPIMYSSKEYGFFSISGNLATQFPQAVGWAMGSAIKGDSRIAAGWIG
                     DGATAEGDFHNAVTFAGVYRAPVILNIVNNQ"
     gene            920..1408
                     /locus_tag="VF589_07100"
     CDS             920..1408
                     /locus_tag="VF589_07100"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX8257383.1"
                     /translation="MSLARLAAAALLLMLSACAYMAPIQDASTFYVMRHLHTPAGATD
                     PDLTEEGRLYAERLAGHFAAEPPVTIFVSNTKRAQQTAAPLAARLGITPIVYDPADTG
                     GLIGEVLKEPAPVLIVGHSNTVPDIVQRLGGQRPGPLVHEDFGDVWRIRGRSTVKTRL
                     GG"
     gene            complement(1503..>2483)
                     /locus_tag="VF589_07105"
     CDS             complement(1503..>2483)
                     /locus_tag="VF589_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX8257384.1"
                     /translation="LGPEVPEEDLIWQDPVPPGRAPSDAQLSEFRSKILDSGFSVGQL
                     VKAAWASASTYRRSDHRGGANGARVRLEPQRGWAVNEPEELGRVLARLDELRGSLSMA
                     DAIVLAGTAAVERAARDAGFNVEVPFSGGRGDASQDWTDAESFAVLEPVADGFRNYLK
                     TKHSVKTEELLLDRASLLGLSVPEMTVLLGGLRVLGANYQDRPEGVFTHRKGQLTNDF
                     FVHLLDNDTFWQVVDETADEEFIGYDRGAMKEGGRQEKWRATRTDLIFGANSQLRATA
                     EVYAESGHEEKFVRDFVKAWTKVMNADRFDLPQNPKLEEPLGIAEPAAAE"
BASE COUNT          367 a          849 c          853 g          414 t
ORIGIN      
        1 cattgattgt tgacgatgtt gaggatgacc ggcgcgcggt agacgccggc gaaggtgacc
       61 gcgttgtgga agtcgccctc cgcggtggcg ccgtcgccga tccagccggc ggcgatgcgg
      121 ctgtcgccct tgatcgccga ccccatcgcc cagccgacgg cctgcgggaa ttgggtggcg
      181 agattgccgg agatggagaa gaagccgtat tccttcgagg aatacatgat cggcagctgg
      241 cgccccttca gcggatcgcc gcggttggaa tagacctggc acatcatctg gacgagcgga
      301 tagtcgcgcg cgaccagcag gccctgctgg cggtaggtgg ggaagcacat gtcgtcgcgg
      361 tcgagcgcgt gggcggcggc gacggcgatc gcctcctcac ccgtcgactt catgtagaag
      421 ctggtcttgc cctgccgctg ggcgcggtac atgcggtcgt cgtagacgcg caccatcgcc
      481 atgtcgcgca gcatccggcg cagcgtgtcg gcgtcgagcc gcgggtccca ggggccgacc
      541 gctttgccct cctcgtcgag cacgcggacc agcgaataag cgaggtcgcg catgtcgtgc
      601 gggctggcgg cgacgtcggg gcgcggcacg ctgccggcgg cggggatggc gatgtggctg
      661 aaatcgactt cgtcgccggg gcgggcttcg ggctcgggga catggagtgc gagtgccgga
      721 cgattgcggc gctcctctgg cttcagcatc ctcacacttt catttctttt cacgagcgga
      781 cccgacccgg ccgcccatcg atgcggcggc cctaatcgat tttttccggc gatgcacccc
      841 ttgtgaaccg aggttgcggg acgcgccgtt ttgcgcgacc taggccgtgt cctgcgcgct
      901 gtttctggag cccttgtcga tgagccttgc ccgcctcgcc gccgccgcct tgctgctgat
      961 gctgtcggcc tgcgcctata tggcgccgat tcaggacgcc tcgaccttct acgtgatgcg
     1021 gcatctgcac acgccggcgg gcgccaccga tcccgacctc accgaggaag ggcggcttta
     1081 tgccgagcgg cttgccggcc atttcgcagc cgagccgccg gtcactatct tcgtcagcaa
     1141 caccaagcgc gcgcagcaga cggcggcgcc gctcgccgcc cggctcggca ttacgccgat
     1201 cgtctacgac ccggccgaca cgggcggcct gatcggcgag gtgctgaagg agccggcgcc
     1261 ggtgctgatc gtcggccaca gcaacaccgt gcccgacatc gtccagcgcc tcggcggcca
     1321 gcggccgggg ccgctggtgc acgaggattt cggggacgtg tggcggatcc gggggcggag
     1381 cacggtcaag acgaggctcg gaggctagcc caccgccccg gcggagaacc ggaccgcgcc
     1441 catggacgcc actggacccc ggctttcgcc ggggaacagt caggcggccg cagcccgcaa
     1501 tgtcactccg ccgcggccgg ctccgcgatg ccgagcggtt cttcgagctt cgggttctgc
     1561 ggcaggtcga agcggtcggc gttcatcacc ttggtccagg ccttgacgaa gtcgcggacg
     1621 aacttctcct cgtggccgct ttccgcatag acctcggccg tggcgcgcag ctggctgttc
     1681 gcgccgaaga tgaggtcggt gcgggtcgcc cgccactttt cctggcgccc cccctccttc
     1741 atggcgccgc gatcgtagcc gatgaactcc tcgtcggcgg tctcgtcgac cacctgccag
     1801 aaggtgtcgt tgtcgagcag gtggacgaag aagtcgttgg tgagctggcc cttcctatgg
     1861 gtgaagacgc cttcgggacg gtcttggtaa ttggccccga gcacgcgcag gccgccgagc
     1921 agaaccgtca tctcgggcac cgacaggccg agcagcgagg cccggtcgag aagcaactct
     1981 tccgtcttca ccgaatgctt ggtcttgagg taattgcgga agccgtccgc gaccggctcc
     2041 agcacggcga agctttcggc atcggtccaa tcctggctgg catcgccgcg accgcccgag
     2101 aacggcactt cgacgttgaa gccggcatcg cgagcggccc gttcgaccgc cgccgtgccg
     2161 gcgagcacga tcgcgtctgc catcgacagc gatccgcgca gctcgtcgag cctggcgaga
     2221 acccgcccca gctcctccgg ctcgttgacc gcccagccgc gctgcggctc gagccggacc
     2281 cgggcgccgt tggcgccgcc gcgatggtcg gaccggcggt aggtcgaggc tgaagcccag
     2341 gccgccttga ccagctgccc gacgctgaac ccggaatcga ggatcttcga cctgaactcg
     2401 gacagctgag cgtcggacgg agcccggccc ggcggaaccg ggtcctgcca gatcagatcc
     2461 tcctccggaa cctccggccc gag
//
LOCUS       DASVRK010000181         6064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_409093, whole genome shotgun sequence.
ACCESSION   DASVRK010000181 DASVRK010000000
VERSION     DASVRK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409093"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="VF589_07110"
     CDS             <1..1665
                     /locus_tag="VF589_07110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX8257385.1"
                     /translation="APLKPWLDRVAAIGSTADLAKAFGELGQYGVASPFGAQVDQDLK
                     NNKVYAAYVGQGGLGLPDRDYYLDDKNAKFVEARAQYQQHVATMLRLAGVSDPEARAK
                     RIYELEKKIATTHWTRAEQRQIDKLYNPMSRTELAQKMPGFDWATYLSAAGVASEPTI
                     IVAHPSALAGAAKLVQSEPLQAWKDYLTFHTIAAAAPLLPKAFVEENFAFDGRTLQGT
                     PQLKDRWKRGVDLVGGGMGEAVGQLYVAKHFPPAAKAKADELVRNLIAAMDKRLTGLE
                     WMAPETKAKARAKLAAFTPKIGYPDTWRDYSGLNVVPGDAVGNALRSTEFEYRRNLNK
                     LGKPVDRGEWGMYPQTVNAYANPLLNEIVFPAAILQPPFFDPNADDAVNYGGIGAVIG
                     HEISHHFDDQGRKFDPKGNLADWWTPADVDRFKVHTDKLVAQYGAYEPLPGAKVNGEL
                     TLGENIADLAGLTIAYDAYRMSLKGREAPVIDGYTGDQRFFLGFGQVWRNKYRDPFLQ
                     QILTTDSHTPGHFRPNVVRNFDPWYAAFDVKDGKLYLPPEQRIRIW"
     gene            1800..3068
                     /locus_tag="VF589_07115"
     CDS             1800..3068
                     /locus_tag="VF589_07115"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8257386.1"
                     /translation="MILTGLIGGRADPLPVDAARSEWPAGSEIGEAQPFVSNIDYARL
                     DARLQQLIAGDKMVGLAVGIVENGEIRFLKGYGVTQAGSNDTVTPSTIFRWASVSKGV
                     AGDMIAKLAEQEKLSLYEPVAKYSASLRLPGGNEHRATISDLLSHRLGLFGHANDAKL
                     EDGGDARLLRANLATLNAICPPGNCHAYQNVAYDAASEIVEKVTGQTYEQAVREQLFL
                     PLGMTSATISRDGLLTARSWARPHRGGKNSKPVEVTDSYYRVPAAGGVNSSVKDLAIW
                     MRAQMGAEPGVLPPKVLANVQSPRANTPGEQARKRKFRERLSMATYGLGWRIYDYAGN
                     TVVGHHGGVTGYRSLIMFDPDRKAGVVALWNSSTSQPGGLEFEVMDMVYQLPFKDWLG
                     LDGRPGAAPATPAEPGNVAAPAVATGNLAS"
     gene            3108..3581
                     /locus_tag="VF589_07120"
     CDS             3108..3581
                     /locus_tag="VF589_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257387.1"
                     /translation="MTALRILRAAIAGAVAWWVGMQILFGQAQALLADPNLQSAKMNA
                     IYELAPPARIAVDPWLLPAGMFVTALLQAACFAFIRRGLPAGLVARGLAFGAVAWGLF
                     TPWFEFYLPWSLMLEPTLLVLVEMLCWAGIMALVGLAISLAFGRDGPRYETTGAC"
     gene            complement(3578..4273)
                     /locus_tag="VF589_07125"
     CDS             complement(3578..4273)
                     /locus_tag="VF589_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8257388.1"
                     /translation="MIRVASYNMRKAIGTDRRRRPERTLDVLNELGADVIALQEADRR
                     FGSRLSALPLRLIEEHSDYKPVRFAARDGSLGWHGNAMLVRKSVEVVDKELVHLPSLE
                     PRGAVLADLRLGGTELRVVGMHLDLSGLWRRRQAHAILAHLAARDGDPATVLMGDLNE
                     WSASGGCLRDFAQSFRFAECGRSFHSRKPIAQLDRIMVGGELEIVACGAHYSPAARRA
                     SDHLPIWAGLEPG"
     gene            complement(4343..4666)
                     /locus_tag="VF589_07130"
     CDS             complement(4343..4666)
                     /locus_tag="VF589_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX8257389.1"
                     /translation="MFIQIHTDNQIDSDNERDSRIEEKVREKLARFESRITDVEIHVS
                     DINGPRGGAADLRATLEARVNGMPPVGVADQGSTIERAIVGAAGKAVRALDHALGKFG
                     DRKGH"
     gene            4789..>6064
                     /gene="radA"
                     /locus_tag="VF589_07135"
     CDS             4789..>6064
                     /gene="radA"
                     /locus_tag="VF589_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX8257390.1"
                     /translation="MAKPRKRYVCQACGSVASRWQGQCGDCAEWNSLVEDAGAVVTPF
                     SARHDLRTGGRRVELVGLDAEIALPPRMRSGMAEFDRAVGGGLVAGSATLIGGDPGIG
                     KSTLLLQAAARVAAAGLPVAYISGEEAADQVRLRARRLGLGAAPVRLAAATSVRDILT
                     TLGEGPPPALLVIDSIQTMHSDLIEGAPGTVSQVRASAQELIRFAKESGTAVVLVGHV
                     TKDGAIAGPRVLEHMVDTVLSFEGERSHQYRILRAAKNRFGGTDEIGVFAMAEEGLAE
                     VANPSALFLTHRGEAVSGATVFPALEGTRPVLVEIQALTVRLASGATPRRAVVGWDSG
                     RLAMILAVLEARCGLSFSTAEVYLNIAGGYRVSDPAADLAVAAALVSALSERPVPAET
                     VAFGEVSLSGELRPVAHGALRLKEAAKLGFERA"
BASE COUNT          930 a         2179 c         1994 g          961 t
ORIGIN      
        1 gccccgctca agccgtggct cgaccgggtc gcggcgatcg gctccacggc cgacctggcc
       61 aaagctttcg gcgaactcgg ccagtacggc gtcgcctccc cctttggcgc gcaggtcgac
      121 caggacctca agaacaacaa agtctacgcc gcttatgtcg gccaaggcgg cctcggcctc
      181 cccgaccgcg attattatct cgacgacaag aacgccaagt tcgtcgaggc gcgcgctcaa
      241 tatcagcagc atgtcgccac catgctgcgc ctcgccggcg tctccgatcc ggaggcccgc
      301 gccaagcgca tctacgagct cgagaagaag atcgcgacca cgcactggac ccgtgccgag
      361 cagcgccaga tcgacaagct ctacaatccg atgagccgga ccgagcttgc gcagaagatg
      421 ccgggcttcg actgggcgac ctatctgagc gccgccggcg tcgccagcga gccgaccatc
      481 atcgtcgccc atccgagcgc gctcgccggc gccgccaagc tggtccagtc cgagccgctg
      541 caggcgtgga aggattatct gaccttccac accatcgccg cggccgcgcc gctgctgccg
      601 aaggcgttcg tcgaggagaa cttcgccttc gacggccgca ccctccaggg cacgccgcaa
      661 ttgaaggacc gctggaagcg cggcgtcgac ctggtcggcg gcggcatggg cgaggcggtc
      721 ggccagctct atgtcgccaa gcacttcccg cccgccgcca aggccaaggc cgacgaactg
      781 gtccgcaacc tcattgcggc gatggacaag cgcctcaccg gcctcgaatg gatggcgccc
      841 gagaccaagg ccaaggcgcg cgccaagctc gccgccttca cgcccaagat cggctatcct
      901 gacacatggc gcgattattc gggcctgaac gtcgtccccg gcgacgccgt cggcaatgcg
      961 ctgcgctcga ccgagttcga atatcggcgc aatctgaaca agctcggcaa gccggtcgat
     1021 cgcggcgagt ggggcatgta cccgcagacc gtgaacgcct acgccaaccc gctcttgaac
     1081 gagatcgtgt tcccggcggc gatcctgcag ccgcccttct tcgatcccaa tgccgacgat
     1141 gccgtcaatt acggcggcat cggcgcggtc atcggccacg agatcagcca ccatttcgac
     1201 gaccagggcc gcaagttcga ccccaagggc aatctcgccg actggtggac tccggccgac
     1261 gtcgatcggt tcaaggtcca taccgacaag ctcgtcgccc aatatggcgc ctacgagccg
     1321 ctgccgggcg cgaaggtcaa cggcgagctg acgctcggcg agaatatcgc cgatctcgcc
     1381 ggcctcacca tcgcctacga cgcctaccgc atgtccttga agggcaggga agcgccggtg
     1441 atcgacggct ataccggcga ccagcgcttc ttcctcggtt tcggccaggt ctggcgcaac
     1501 aaatatcgcg atccgttcct ccagcagatc ctgaccaccg actcgcatac gccgggccat
     1561 ttccggccga acgtcgtgcg caacttcgat ccctggtatg cggcgttcga cgtcaaggac
     1621 ggcaagcttt accttccgcc ggaacagcgt atcagaatct ggtaagcgcg aatcccgggc
     1681 cggcggcggc gctgccggcc cggatcgcgt ccagtttgta aagttcgagg agaggtctgc
     1741 tgacccggcg gatccaatat gcttcggcgg ccgcgctcgc ggtcgtcctg ctcgtcatca
     1801 tgattttgac tgggctgatc gggggacggg ccgatccgct cccggtcgac gccgcgcgtt
     1861 cggaatggcc ggccggcagc gagatcggcg aggcccagcc gttcgtcagc aacatcgatt
     1921 acgcgcgcct cgacgcccgg ctgcagcaat tgatcgccgg cgacaagatg gtcggcctcg
     1981 ccgtcggcat cgtcgagaat ggcgagatcc gctttttgaa aggctatggc gtcacccagg
     2041 ccggcagcaa tgatacggtg acgccgagta ccatcttccg ctgggcgtcg gtctccaagg
     2101 gcgtcgccgg cgacatgatc gccaagctcg ccgagcagga gaaattgtcg ctctacgagc
     2161 cggtcgccaa atattcggcc tcgctgcggc tgcccggcgg caacgagcat cgcgccacca
     2221 tctccgattt gctctcgcac cggctcggcc tgttcggcca cgccaacgat gccaagctcg
     2281 aagatggcgg cgacgcgcgg ctgctgcgcg ccaacctcgc cacgctcaac gccatctgcc
     2341 cgcccggcaa ttgccacgct taccagaatg tcgcctatga cgcggccagc gagatcgtcg
     2401 agaaggtcac cggccagacc tacgagcagg cggtgcgcga gcaattgttc ctgcccttgg
     2461 ggatgaccag cgccaccatc tcgcgcgacg gcctgctgac ggcgcgcagt tgggcccgcc
     2521 cccaccgcgg cggcaagaat tccaagccgg tcgaggtgac cgattcctat tatcgcgtgc
     2581 cggcggcggg cggggtcaat tcctcggtca aggaccttgc catctggatg cgcgcgcaaa
     2641 tgggcgccga gcccggcgtg ctgccgccga aggtgctcgc caacgtccag tcgccgcgcg
     2701 ccaacacgcc gggcgagcag gcccgcaagc gcaagttccg cgagcggctg tccatggcca
     2761 cctacgggct cggctggcgc atctacgatt atgccggcaa caccgtcgtc ggccatcatg
     2821 gcggcgtcac cggctatcgc tcgctgatca tgttcgatcc cgacaggaaa gccggcgtgg
     2881 tggcgctgtg gaacagctcg acctcgcagc cgggcgggct cgaattcgaa gtcatggaca
     2941 tggtctacca gctgccgttc aaggactggc tcggcctcga cggcaggccc ggcgccgcgc
     3001 ccgccacgcc ggccgagccc ggcaacgtgg cggcgcctgc ggtagccacc ggcaatctgg
     3061 cgagctgagc cgcgcgcgcg gctccgggcg aagggagtga acgcgacatg acggccttgc
     3121 gcatcctgag agcggcgatc gccggggcgg tcgcctggtg ggtcggcatg caaatactct
     3181 tcggccaggc gcaggcgctg ctcgccgatc cgaacctgca aagcgccaag atgaacgcca
     3241 tttacgagct cgccccgccg gcgcgaatcg cggtcgatcc gtggctgctt ccggcgggga
     3301 tgttcgtcac cgccttgtta caggccgcct gcttcgcctt catccggcgc gggcttccgg
     3361 ccggcctggt cgcgcgcggc ctcgccttcg gcgcggtcgc ctggggcctg ttcacgccct
     3421 ggttcgaatt ctacctgccg tggagcctga tgctggagcc gaccttgctg gtgctggtcg
     3481 agatgctctg ctgggccggc atcatggccc tggtcggcct cgccatctcg ctagcgttcg
     3541 gccgcgacgg cccgcgatac gagaccaccg gcgcctgcta gcccggttcc agtcccgccc
     3601 agatcggcaa atggtcggac gcccgccgcg ccgccgggct gtaatgggcg ccgcacgcga
     3661 cgatctcgag ctcgccgccg accatgatcc ggtcgagctg cgcgatcggc ttgcgcgaat
     3721 ggaagctgcg gccgcactcg gcgaagcgga aactttgcgc gaaatcgcgc aggcagccgc
     3781 cgctggcgct ccattcgttg aggtcgccca tcagcactgt cgctggatcg ccgtcgcggg
     3841 cggcgagatg ggcgaggatc gcatgcgcct gccgccgccg ccacaggccg gacaggtcga
     3901 gatgcatgcc gaccacgcgc agctccgtcc cccccagcct caagtccgcc agcaccgcgc
     3961 ccctgggctc gagcgagggc aggtggacca gctccttgtc gaccacttcg accgactttc
     4021 gcaccagcat cgcattgccg tgccagccga ggctgccgtc gcgcgcggcg aagcgcaccg
     4081 gcttatagtc gctatgctcc tcgatcagcc tcagcggcag cgccgacagc cgcgatccga
     4141 accgccggtc cgcctcctgc agcgcgatca cgtcggcacc gagctcgttc agcacgtcga
     4201 gcgtgcgctc gggccgccgg cgccggtcgg tgccgatcgc cttgcgcata ttgtagctcg
     4261 cgacccggat catcgcccgc cgcatagggg cgcttgccgc ccgcgcgcaa cgctcatcgc
     4321 cgggccggcg gccaaggccg gctcaatggc ccttgcggtc gccgaacttg ccgagcgcgt
     4381 ggtcgagcgc gcgcaccgcc ttgccggcgg cgccgacgat ggcgcgctcg atggtcgagc
     4441 cctggtcggc gacgccgacc ggcggcatgc cgttgacccg cgcctccagc gtcgcgcgaa
     4501 ggtcggcggc gccgccgcgc gggccgttaa tgtcggacac gtggatctcg acatcggtga
     4561 tccggctttc gaaccgcgcc agcttttccc ggaccttttc ctcgatgcgg ctgtcccgct
     4621 cattgtcgct gtcgatctgg ttgtcggtat ggatctggat gaacaaggtg ggtctccgtt
     4681 gattgcttgc cggtccaatg ccggggcagg gcaggccgtt ccaagccgcc gccgccgccc
     4741 gtctcaaaaa cactggctga ccgccgcgcg cgcggctagt tccggcggat ggccaagccc
     4801 cgcaaacgct atgtctgcca ggcctgcggc tcggtcgcga gccgctggca ggggcagtgc
     4861 ggcgactgcg ccgagtggaa cagcctggtc gaggatgccg gcgcggtggt gacgcccttc
     4921 tcggcgcgcc acgatctgcg caccggtggt cgccgcgtcg agttggtcgg cctcgacgcc
     4981 gagatcgcgc tgccgccgcg gatgcgcagc ggcatggccg agttcgaccg cgccgtcggc
     5041 ggcgggctgg tcgcgggctc ggccacgttg atcggcggcg atcccggcat cggcaagtcg
     5101 actttgctgc tccaggccgc cgcccgcgtc gccgccgccg ggctgccggt cgcttatatc
     5161 tccggcgagg aggcggccga ccaggtccgg ctgcgcgccc gccggcttgg cctcggcgcc
     5221 gcgccggtcc ggctcgccgc cgccacttcg gtccgcgaca tcctcaccac tttgggcgag
     5281 gggccgccgc ccgccctgct ggtgatcgat tcgatccaga ccatgcattc cgatctgatc
     5341 gagggcgctc ccggcacggt cagccaggtc cgcgcctccg cccaggagct cattcgcttc
     5401 gccaaggaga gcgggacggc cgtggtgctg gtcggccacg tcaccaagga cggcgccatc
     5461 gccggcccgc gcgtgcttga acatatggtc gacacagtgc tgagcttcga aggcgagcgc
     5521 agccaccaat atcgcatcct gcgcgccgcc aagaaccgct tcggcggcac cgacgagata
     5581 ggcgtgttcg cgatggcgga ggaggggctg gccgaagtcg ccaaccccag cgccctgttc
     5641 ctcacccatc gcggcgaggc cgtctccggc gccaccgtct tcccggcgct cgaaggcacc
     5701 cggccggttt tggtcgagat ccaggcattg accgtccgcc tcgccagcgg cgccaccccg
     5761 cgccgcgcgg tggtcggctg ggattcgggg cggctggcga tgatcctggc ggtgctggag
     5821 gcgcgctgcg gcctcagctt ctcgaccgcc gaagtctatt tgaacatcgc cggcggctac
     5881 cgggtcagcg atcccgccgc cgatctcgcc gtcgccgcgg ccctggtctc ggcgctgtcg
     5941 gagcggccgg tgccggcgga aaccgtggcg ttcggcgaag tctcgctctc gggcgagctg
     6001 cgcccggtcg cccacggcgc gctccggctc aaggaagccg ccaagctcgg cttcgagcgc
     6061 gcct
//
LOCUS       DASVRK010000182         4041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_96156, whole genome shotgun sequence.
ACCESSION   DASVRK010000182 DASVRK010000000
VERSION     DASVRK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96156"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VF589_07140"
     CDS             <1..305
                     /locus_tag="VF589_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8257391.1"
                     /translation="FTRSDHYRFVQEGIPSVFLMTGFGNGGDKQFTNFLATNYHKPSD
                     DLSQPINWEAGAKFARINYLIARDIADAPQAPLWYAGSFFGETFAAGQPKARAGGN"
     gene            complement(440..1405)
                     /gene="pip"
                     /locus_tag="VF589_07145"
     CDS             complement(440..1405)
                     /gene="pip"
                     /locus_tag="VF589_07145"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX8257392.1"
                     /translation="MADSPAQTLRTLYPPIEPYKSGHLDVGDGHGVYWELCGNPEGKP
                     AIFLHGGPGAGCNPDHRRQFDPARYNILLFDQRGCGRSTPHADLEANTTWHLVADMER
                     LREMIGVERWLVFGGSWGSTLALAYAETHAERVSELVLRGIFTLRRFEIDWFYQHGTS
                     ELFPDKWERFVAPISEGERAELVAAYHRRLTSSDRAVQLEAAKAWSQWEGETITLLPD
                     PKTAAAFTADDFAIAFARIENHYFVHHGWMEEGQLLRDAGKLKGIPGVIVQGRYDVCT
                     PARTAWDLHHAWPEAEFHLIPDAGHAYNEPGILDRLIRATDAFAG"
     gene            1493..>4041
                     /gene="clpB"
                     /locus_tag="VF589_07150"
     CDS             1493..>4041
                     /gene="clpB"
                     /locus_tag="VF589_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX8257393.1"
                     /translation="MNLEKFTERARGFLQSAQTVALRMNHQRIAPAHVLKALLEDEQG
                     MASGLIARAGGSAPAALKATDAALARIPAVSGSGANAAPALDGETIRLLDQAEQIAQK
                     AGDSYVTVERMLLALVLAKATDAGKALAEAGVKAEALNAAINELRGGRTADTQSAEDR
                     YDALKKFARDLTQAARDGKLDPVIGRDEEIRRTIQILARRTKNNPVLIGDPGVGKTAI
                     AEGLALRIANGDVPDSLKGRRLMSLDLGSMIAGSKYRGEFEERLKGVLDEVKGAEGDI
                     VLFIDEMHTLIGAGKSEGAMDASNLLKPALARGELHCIGATTLDEYRKHVEKDPALQR
                     RFQPVFIGEPTVEDTISILRGLKEKYELHHGVRITDGAIIAAATLSNRYITDRFLPDK
                     AIDLMDEAASRLRMEVESKPEEIETLDRRIIQLKIEREALKRENDRASKDRLAALEEE
                     LANLEQQSAELTGRWQAEKEKIAGESKLKEQLDQARLELEQAQRAGDLARAGELAYGR
                     IPGLEKQLAETEAATAGAMLREEVTGEDIAGVVSRWTGIPVDRMLEGEREKLLAMEDA
                     LGKRVIGQQDAVKAVASAVRRSRAGLQDPNRPLGSFLFLGPTGVGKTELTKALAEFLF
                     DDPHAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGVLTEAVRRRPYQVILFDEVEK
                     AHGDVFNILLQVLDDGRLTDGQGRTVDFANTLIILTSNLGSQYLSSLTDGQRVEEVEP
                     QVMEVVRAHFRPELLNRLDEIILFHRLAAGHMGPIVDIQIVRLGKLLADRKIRLELTD
                     GARAWLGRVGYDPVYGARPLKRAVQKHLQDPLADALLRGEVRDGQTVRVEEG"
BASE COUNT          715 a         1269 c         1458 g          599 t
ORIGIN      
        1 tgttcacccg ctccgatcac taccgtttcg tgcaggaggg aatcccctcg gtgttcctga
       61 tgaccggttt cggaaatggc ggcgacaagc agttcaccaa cttcctcgcg accaactacc
      121 acaagccttc ggacgatctc agccagccga tcaactggga ggcaggcgcc aagttcgcgc
      181 ggatcaacta tctgatcgcc cgcgacatcg ccgacgcgcc gcaggcgccg ctttggtatg
      241 cgggcagctt cttcggcgag accttcgcgg ccgggcagcc gaaggcgagg gcgggcggga
      301 attaggcctc ggaagatcgg cgtcgattga ttgcctcacg gtcgtcaccc cggacttgat
      361 ccggggtccc gcttcttctt tcgctgagtg cagaaggaga agcgggatgc cggatcaagt
      421 ccggcatgac gaagcggcgc tagcccgcga aggcgtcggt ggcgcggatc aggcggtcga
      481 ggataccggg ctcgttatag gcgtggccgg catcggggat caggtggaat tcggcctcgg
      541 gccaggcatg atgcaggtcc caggcggtgc gggcgggggt gcagacgtcg tagcggccct
      601 ggacgatcac gcccggtatc cccttgagct tgcccgcgtc gcggaggagc tgcccttctt
      661 ccatccagcc gtgatggacg aaataatggt tctcgatgcg cgcgaaggcg atcgcgaaat
      721 cgtcggcggt gaaggcggcg gcggtcttcg ggtcgggaag cagggtgatg gtctcgccct
      781 cccactggct ccacgccttg gccgcctcga gctggacggc gcggtcggag ctggtcaggc
      841 ggcggtggta ggcggcgacc agctcggccc gctcgccctc gctgatcggg gcgacgaacc
      901 gctcccattt gtccgggaac agctcggacg tgccatgctg gtagaaccag tcgatctcga
      961 agcgcctgag cgtgaagatg ccgcgcagca ccagctcgct cacccgttcg gcatgggttt
     1021 cggcataagc cagggcgagc gtcgagcccc aggagccgcc gaacaccagc cagcgctcga
     1081 cgccgatcat ctcgcgcagg cgctccatgt cggcgacgag gtgccacgtg gtgttggcct
     1141 cgaggtcggc atgcggggtc gaccggccgc agccgcgctg gtcgaacagc aggatgttgt
     1201 agcgcgcggg gtcgaactgc cggcggtggt cgggattgca gccggcgccg gggccgccat
     1261 ggaggaatat ggccggcttg ccctcggggt tcccgcacag ctcccaatag acgccatgcc
     1321 cgtcgccgac gtcgagatga ccgctcttgt aaggctcgat cggggggtag agcgttcgca
     1381 gcgtctgcgc gggcgagtcg gccaaggttc gtctccaaat atttcggcga ggcagagcac
     1441 ttgtgttgcc taaacgcaac actaaatcgg cggtgaagga gatgaggggc ttatgaacct
     1501 ggagaaattc accgaacgcg cgcgcggatt cctgcaatcg gcgcagaccg tggcgctgcg
     1561 catgaaccat cagcggatcg cgcccgcgca tgtgctgaag gccttgctcg aggacgagca
     1621 ggggatggcc tccgggctga tcgcacgggc cggaggcagc gcgccggcgg cgctcaaggc
     1681 gaccgatgcc gcgctcgcca ggataccggc ggtcagcggc agcggcgcga atgccgcgcc
     1741 cgcgcttgac ggcgagacca tccgcctgct cgaccaggcc gagcagatcg cgcaaaaggc
     1801 gggcgacagc tatgtgactg tggagcggat gctgctcgcc ctggtgctgg ccaaggcgac
     1861 cgacgcgggc aaggcgctgg ccgaggcggg cgtcaaggcc gaggcgctga acgcggccat
     1921 caacgagctt cgcggcgggc gcaccgccga cacccagtcg gccgaggacc gctacgatgc
     1981 gctcaagaag ttcgcgcgcg acctcaccca ggcggcgcgc gacggcaagc tcgatccggt
     2041 gatcggccgc gatgaggaga tccgccgcac catccaaatc ctcgcccggc gcaccaagaa
     2101 caatccagtg ctgatcggcg accccggcgt cggcaagacc gcgatcgccg aggggctggc
     2161 gctgcgcatc gccaatggcg acgtgccgga cagtctgaag ggccggcgac tgatgtcgct
     2221 cgacctcggc tcgatgatcg ccggctccaa atatcgcggc gagttcgagg agcggctcaa
     2281 aggcgtgctc gacgaggtca agggcgccga gggcgacatc gtcctgttca tcgacgagat
     2341 gcatacgctg atcggcgccg gcaagagcga gggcgcgatg gacgcctcga acctgttgaa
     2401 gccggcgctc gcccgcggcg agctgcactg catcggcgcg acgacgctcg acgaatatcg
     2461 caagcatgtc gagaaggacc cggcgttgca gcggcgcttc cagccggtct tcatcggaga
     2521 gccgacggtc gaggacacga tctcgatcct gcgcgggctc aaggagaaat atgagctgca
     2581 ccacggcgtg cggatcaccg acggcgcgat catcgcggcc gcgaccctgt ccaaccgcta
     2641 catcaccgac cgcttcctgc ccgacaaggc aatcgacctg atggacgagg cggcgagccg
     2701 cctcaggatg gaggtcgaat cgaagcccga ggagatcgag acgctcgacc ggcgcatcat
     2761 ccagctgaag atcgagcgcg aggcgctgaa gcgcgagaac gaccgcgcgt ccaaggatcg
     2821 gctggcggcg ctggaggagg agctcgccaa tcttgagcag cagtcggccg agctgaccgg
     2881 acggtggcag gctgagaagg agaagatcgc cggcgaatcg aaattgaagg agcagctcga
     2941 ccaggcgcgg ctcgagctcg agcaggcgca gcgcgccggc gatctcgctc gggcgggcga
     3001 gctggcttac ggccgcatcc ccggccttga gaagcagctc gccgagacgg aggccgcgac
     3061 cgcgggcgcg atgctgcgcg aggaagtcac cggcgaggac attgccggcg tggtctcgcg
     3121 ctggaccggc atccccgtcg accggatgct ggagggcgag cgcgagaagt tgctggcgat
     3181 ggaagacgcg ctcggcaagc gggtgatcgg ccagcaggat gcggtgaaag cggtcgcctc
     3241 ggcggtgcgc cggtcgcggg ccgggctgca ggacccgaac cggccgctcg gctcgttcct
     3301 gttcctgggg ccgaccggcg tcggcaagac cgagctcacc aaggcgctcg ccgaattcct
     3361 gttcgacgac ccgcacgcga tggtgcgaat cgacatgtcg gaattcatgg agaagcactc
     3421 ggtcgcgcgg ctgatcggcg ccccgcccgg ctatgtcggc tatgaggaag gcggcgtgct
     3481 gaccgaggcg gtgcggcggc ggccctacca ggtgatcctg ttcgacgagg tcgagaaggc
     3541 gcatggcgac gtgttcaaca tactcctgca ggtgctcgac gacggccggc tgaccgacgg
     3601 ccaggggcgg acggtggact tcgccaacac gctgatcatc ctgacctcga acctcggctc
     3661 gcaatatctg tccagcctca ccgacggaca gcgcgtggaa gaggtcgagc cgcaggtgat
     3721 ggaggtggtg cgggcgcatt tccggccgga attactcaac cggctcgacg agatcatcct
     3781 gttccaccgg ctggcggccg ggcatatggg gccgatcgtg gacatccaga tcgtccggct
     3841 gggcaagctg ctggcggacc ggaagatccg gctggagctg accgacggcg cccgcgcctg
     3901 gctcgggcgg gtcggctatg atcccgtcta tggcgcgcgg ccgctgaagc gggcggtgca
     3961 gaagcatctg caggacccgc tggcggatgc cttgcttcgc ggcgaggtgc gcgacggaca
     4021 gacggtgcgc gtggaggaag g
//
LOCUS       DASVRK010000183        16923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_593475, whole genome shotgun sequence.
ACCESSION   DASVRK010000183 DASVRK010000000
VERSION     DASVRK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16923
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593475"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..222
                     /locus_tag="VF589_07155"
                     /pseudo
     CDS             1..222
                     /locus_tag="VF589_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685269.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
     gene            complement(480..2417)
                     /locus_tag="VF589_07160"
     CDS             complement(480..2417)
                     /locus_tag="VF589_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8257394.1"
                     /translation="MTKPRHIFACALLLGAAACSNAPGGGGSAKDSYARGLAALEQGQ
                     PRTARVELMNAIQADPNNGAIRVAQARTYLSLNDGVAAEAEIERARKLGVAVDDTRHL
                     LAHALLLRGEPKRALEELNGVPAAHAGYAARLRGQALAKLGDNAGAAAAFKEAIAADP
                     KDNAAWTEVARFRRGSGDLAGAVEAADRALKINAKDIEALTLRGELTRSQYGLRAALP
                     WFDRALEIDPSNIPAMLERAATLGDLGDTKAMLAETRKVLKLSENHPMAFYLQAMIAA
                     RAKKYELARTLYQRTGGALDGEPAGMLLASAIDYQTGNVEQAANRLEKLIGMQPYNRK
                     ARRMLAAAQFKLGDNEATIRTLRPLADRPDADSYTLALIGKAIEKQGDTQAASRYLAR
                     AAQPQRRASTALWANPVSDDQLIALRRFAASKPGHAPSQVLLIGGLLSRGMGQEALDL
                     ALKLQADNPGAPDAHILVGDARGMGGDFKGAAAEYGKAANLAFNEPVAMRMIEALQRS
                     GQGPAAAKVLNLFLEQNPRSVPAQILAATGFLQARQWGEAIRIYEGLRKRLGDRDAVM
                     LNNLAWAYSEQGNYARAIPLAQKAWSLDKDNPATADTLGWLLYKSGKDKAQGLVLLER
                     ASRGAPTDAQIRQHLDAARRG"
     gene            complement(2414..3793)
                     /gene="prsR"
                     /locus_tag="VF589_07165"
     CDS             complement(2414..3793)
                     /gene="prsR"
                     /locus_tag="VF589_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEX8257395.1"
                     /translation="MNAPLKNCVAKAKLLIVEDDEGLQRQLRWAYDDYEVLVASDRNA
                     AVDLLRSQEPAVVTLDLGLPPDPDGVSEGFATLEKILSLKPDTKVIVASGHGARESAL
                     RAISSGAYDFYQKPVDIDQLGLIVRRAFQLHGIENENRRLEEQIGEDRTVLGTMITAA
                     PEMLKVARTIERVAHAGVSVMLLGASGTGKELLAKGLHQSSGRRAGAFVAINCAAIPE
                     NLLEAELFGYEKGAFTGAIKTTEGKIELAHGGTLFLDEVGDIPLPLQVKLLRFLQERV
                     IERIGGRKPIPVETRIVCATHQDLPEMIKAGSFREDLYYRLAEIVIRIPTLAERHGDA
                     TLLAKHFLNRFSQEMNPAVKGFAPSALTAINGWPWPGNVRELENRMKRAVIMAEGKLI
                     TAEDLDLGEGEDQPLPVNLKAVREEADRKAIRQALARTESNITNTAKLLGISRPTLYD
                     LLKQYGLQP"
     gene            complement(3790..5874)
                     /gene="prsK"
                     /locus_tag="VF589_07170"
     CDS             complement(3790..5874)
                     /gene="prsK"
                     /locus_tag="VF589_07170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEX8257396.1"
                     /translation="MIGFIGLWSHALAALLFGALAIWQLRHWNGDQRNRPLVAAFAVT
                     AVWAIFVALLGPHHLLSGLSECARNFAYLAFMHGIVRSAAEDERQRAVKLVYFTVAAV
                     IGLQIVVAGVLPRFEGNPIVHEALTATAYILGLTTAAGSLVLVHNLYGQASPDSRWGI
                     RLPMLALAGMWTYDLHLYTVAYLTRGPVDDLFAMRGAIIAMLVPLFTLASKRNSQWRM
                     QISRAATFQSISVMAILAYLILMMSATQALEVIGGEWVRIGQIGIIFGMTLAALIFLP
                     SGRARAWLRVILAKHFFEHRYDYRQEWLRFTRTVGLGGSQAAPLGDRVIKALADISGS
                     PGGLLLLLDDRHRLTAGARWNWHHDFGQREASDHHFIRFLETTNHVIDFHGSGGRLKS
                     GKREHVDIPAWIDPAGAAWAGVPLLHNDRLVGLVVLEHPLVRRPLDWEDFDLFRTAGI
                     QAASYLAEARSQDALANAERFDEFNRRFAFILHDIKNLVSQLSLVARNAERHAENPEF
                     RADMIATLQSSVKKMNDLLARLSPGNHSEPPRISAVDLHAILAGLVGIKKRGHPVELF
                     GEPGLSALADPGQLEQALAHLVQNAIDASGADEPVRVCCGTRGSEIVVEVIDSGAGMS
                     PDFVSHRLFQPFASTKDGGFGIGAFEARSLITAMGGRVEVESREGEGSRFTIFLPAAE
                     SESSPVYERMRA"
     gene            complement(5874..7262)
                     /locus_tag="VF589_07175"
     CDS             complement(5874..7262)
                     /locus_tag="VF589_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEX8257397.1"
                     /translation="MIRLFKHYVPHTVLLLGLIDFVTLMLAAEAGWLLRDWQIGSKIA
                     LDPGRLPNMLTFAITLQLAMTGVGVYAVESLQSLRHAAPRLLVAVSLGALLLSLVFFV
                     APPLTFWRSNLLYAMLFAIAGLIGIRIALGRSLGSEAFKRRILVLGAGPRAARLEALA
                     RRDGAGFTIAGYVAMNDGPAAVAHAVNRDAISNLSDFVVRLRASEVVLAIEERRNALP
                     LADLLRVKTTGVHVNDLSSFLERETGRVDLDSLNPSWLIFSDGFSSGRRLSSAGKRLF
                     DILVSAAILIITGPAILLTALLVKLESPGPAFFRQRRVGLYGQGFDILKLRSMRQDAE
                     VGGVAVWAQKDDPRVTRVGAIIRKLRIDELPQAWSVLKGEMSFVGPRPERPQFVADLE
                     ARLPYYAERHMVKPGITGWAQINFPYGASVEDARQKLEYDLYYAKNYTPFLDILILLL
                     TIRVVLWPEGAR"
     gene            7588..8031
                     /locus_tag="VF589_07180"
     CDS             7588..8031
                     /locus_tag="VF589_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257398.1"
                     /translation="MAGMFASAPAFACRSPKPKDQAGYLKVINGLFAAWFARDYPEFR
                     KFFLHPELDEPFASQNIFHEHYDDERPRQRGSLMFNGPSAIVQVIAVQPADSWSCGGH
                     GWAELMLVKFFLGIEAPVVAELRYLDGATLAKGEWTPQSPGAAKR"
     gene            complement(8464..8754)
                     /locus_tag="VF589_07185"
     CDS             complement(8464..8754)
                     /locus_tag="VF589_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257399.1"
                     /translation="MTTRPPFKLSRLRDIGWSRWDPIGLSGMESTPDDEYDSYLLEAA
                     GRLWNGASEEDVADYLVSVETRDMGLGDAPGVRPRAGEVVNALSGYLSELRG"
     gene            complement(8803..10239)
                     /locus_tag="VF589_07190"
     CDS             complement(8803..10239)
                     /locus_tag="VF589_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX8257400.1"
                     /translation="MAASAGSHEQRSEMFGHPRGLTVLASTEMWERFSFYGMQALLML
                     YMTQYLLLPQRADEVLGLGAFRSALVDLTGPMTDLAFAAQTFGIYSGLLYGTPLLGAW
                     LGDRVFGKTRTIIVGCLLMAAGHLAMAFEHLFLIALMLIILGAGGVIGNMAAQVGLLY
                     APDDNRRTRAFGVYLITLNMGALAAPLVIGTLGEKVGWHYGFGAAGIGMVIGLVTYLA
                     GRRHLPPDRVTARAERTRLTRSEWRRVGAVLCLLIPFMLYTAAAMQAYGIMFVWADTA
                     VDRVVLGWEVPVTWIGVVDGLLTILGVVIANRIWIRLARRGREPRDLTKIAIGYAGIA
                     IAFLFAGAIATLPLVPVALWIAFFIILDFNFGWIEPPMQSLVSRDSPASVNATMMALL
                     KASTMFSYFLLGWLGRFYEPLGAPLYWTATGALAAAALLLVLVLRKPVMTLLEPTGEF
                     GATPEVAAAADQKEALAASVIAPQPNSS"
     gene            complement(10320..10658)
                     /locus_tag="VF589_07195"
     CDS             complement(10320..10658)
                     /locus_tag="VF589_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257401.1"
                     /translation="MAYHEAPEGSVADDEREPPIEDFQVRYARLGQRFPEYGFYALTD
                     PVGPLHETGRIGDSIYDLADVAEDLDEVVWRHENIGPDDAHWHFKLWFRGYWGTPLRN
                     LSGYLHARTW"
     gene            complement(10829..11224)
                     /locus_tag="VF589_07200"
     CDS             complement(10829..11224)
                     /locus_tag="VF589_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257402.1"
                     /translation="MRAADFERDGWCLEDGEERRREAPATFWLPELTDRQSLQPGDLV
                     KLIFRLAVDNDEEPVAVERMWVIVRERVGDQYLGILDNDPRAIGENEELWSGVELPFS
                     ARHVIDIRRDPAEGSEPAPLPTRFWDRDP"
     gene            complement(11831..12349)
                     /locus_tag="VF589_07205"
     CDS             complement(11831..12349)
                     /locus_tag="VF589_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257403.1"
                     /translation="MSATVESGHRGSQAAGMTKRSRVRSAAILLAAIIGTSCGPAAPR
                     KILTVEEVVARIDELDGRTVSVAGYLPECGGNNCILYRSKADSHQWDRVKVALSANRR
                     VAIPDVPALGIGSGTNFEFDAKAAPFTHGYVVITGTITNECRFEGKPACTDRGPDLEP
                     ETIAAGGPPARS"
     gene            13159..13911
                     /locus_tag="VF589_07210"
     CDS             13159..13911
                     /locus_tag="VF589_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8257404.1"
                     /translation="MRIVGSYVSPYARKVLACLALKSVAYEIDPITPFYGNDEFERLS
                     PLRRIPLLIDGDFVLGDSSVICAYLDEAYPGPPLLPADARERARARWIEEYADTRLGD
                     IFIWGLFYQKIVHPIVWGEPGDAARIERTLSVDAPAACDWLERQLGERAVADGFLFGA
                     DIGLADIAIASFFRNAAYAGFAPDPAGWPLLAGFLERALAHDCFACLLPFEQAQLSTN
                     PQGRRAALLAAGAPLSAQTMGTRVPRRGVMRL"
     gene            13969..14430
                     /locus_tag="VF589_07215"
     CDS             13969..14430
                     /locus_tag="VF589_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257405.1"
                     /translation="MSAFAALAMMTVAPPCAPGPLTPNAPIPPPERRVDGRSSAPYGD
                     ALGDAWEMEEVACWRGIWQRRGEGRLWDGYWFHPGGERVRATLEMWRDGRSVIVVRRH
                     PGGQYCRYDGTISADWWTIEGRYTCTWERTPMPWRAEIVRLEHAEPALLRR"
     gene            14595..15518
                     /locus_tag="VF589_07220"
     CDS             14595..15518
                     /locus_tag="VF589_07220"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX8257406.1"
                     /translation="MFETKIDRDHAWPQKTRELHSHHFDSTIWNDFRFRDDDIVIGTY
                     AKSGTTWTQQIVAQLVFNGAEGINVGEMSPWMDLRLPPKEVKLPLVEAQTHRRFLKTH
                     LPVDALVFSPDAKYIYIGRDGRDVVWSMYNHHSNFVPETYQALNDTPGRVGPPLGLPP
                     ASIRDYFNEWVERDGYPFWSFWENIRSWWEIRHLPNVMLLHFSELKADMPGQIRRIAD
                     FLEIAVDEARWPAILDHCSFDYMKRNAELAAPLGGVLWQGGAQTFIHKGVNGRWRDVL
                     SADDCARYEAMAAEQLGADCARWLASGAHTG"
     gene            15662..16786
                     /locus_tag="VF589_07225"
     CDS             15662..16786
                     /locus_tag="VF589_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8257407.1"
                     /translation="MSAAGPVSARFAGIGPRNPRTRGAIAVCLLFYAFTLMVFTVAGF
                     FRGPESFRESLSIGVPMMIVDGFLSFQIYFLLGPASRLPAVPRWSLIVAGVVAIALLQ
                     AVWDTQLRLWAGSVDQAGAYLAYVRSATLNVYNTGMFAALIAFQGAYLDLSEHQRLLN
                     ASRESERDAHMLALRFQLNPHFLFNTLNAISSLVIVGRAAHAEAMIDRLSSFLRASLT
                     ADPHSLVSIDEEFEMLDSYLEIESVRFGDRLVTAIDLPPPLTRALMPPFLLQPLVENA
                     IKYAVAPSTRPVEVRIAAEEQDGRLVLTVEDDGDGCGNGSPGTGVGLANVRERLRLNY
                     GSRARLEMQQDGSGCRVRIVLPLELPDESVAAARPADVFA"
BASE COUNT         2729 a         5623 c         5736 g         2835 t
ORIGIN      
        1 aaggaccagc gcgactaaga tctgctgccc aagcggatcg aggagatcga ggcgcagatc
       61 gcccgcgccg aggctgcgct ttccgacccc gacctctaca ctcgggatgc cgggcgcttc
      121 gccgccctca ccgccgccat cgaatccgcc cgcaccgaac gcgacgcggc ggagcagagg
      181 tggctggaac tggccgagca ggtcgaggcg ctggcgggct agagcttgat gagctcacgc
      241 tagcccgctc atcctgagga gccgctgagc ttggcgaagc ggcgtctcga aggacgcaca
      301 atgatccttc gagacgggcc ttcgccttcg ctcagtccct cctcaggatg agcgaaaatg
      361 agttgcattc gaacctgctt aaatcgtcac cccggacttg atcctgggtc ccgcttcttc
      421 acgtcaccgt cgcgagaaaa agaagcggga ttccggatca ggtccggcat gacgcccctc
      481 tacccccgcc gggcggcgtc gagatgttgc cttatctgcg cgtcggtcgg cgcgccgcgc
      541 gaggcgcgtt cgaggaggac gaggccttgc gccttgtcct tgccgctctt gtagagcagc
      601 cagccgagcg tgtccgccgt cgccggattg tccttgtcga ggctccacgc cttttgcgcg
      661 agcgggatcg ccctggcgta attgccctgc tcggaatagg cccaggcgag gttgttgagc
      721 atcaccgcgt cgcggtcgcc gaggcgcttg cgaaggccct cgtagatgcg gatcgcctcg
      781 ccccactggc gcgcctggag gaatccggtc gcggcgagga tctgggccgg aacgctgcgc
      841 ggattctgct cgaggaacag gttcagcacc ttggccgcgg ccggaccctg gccggaacgc
      901 tgcagcgcct cgatcatccg catcgccacc ggctcgttga aggcgaggtt ggcggccttg
      961 ccatattcgg ccgccgcgcc cttgaagtcg ccgcccatgc cgcgcgcgtc gccgaccaat
     1021 atgtgggcgt cgggcgcgcc gggattgtcg gcctgcagct tcagcgccag gtccagcgcc
     1081 tcctggccca tgccgcgcga cagcaggccg ccgatcagca gcacctgcga gggggcgtgg
     1141 ccgggcttgc tggcggcgaa gcggcggagc gcgatcagct ggtcgtcgct gaccggattg
     1201 gcccagagcg cggtcgacgc gcggcgctgc ggctgggcgg cgcgggcgag gtagcgcgag
     1261 gcggcctggg tgtcgccctg cttctcgatc gccttgccga tcagcgccag cgtgtagctg
     1321 tcggcgtccg gccggtcggc gagcgggcgc agcgtgcgga tcgtcgcttc attgtcgccg
     1381 agcttgaact gcgcggcggc cagcatgcgg cgcgccttgc ggttgtaggg ctgcatgccg
     1441 atcagctttt cgagccggtt ggcggcctgt tcgacattgc cggtctgata atcgatcgcg
     1501 ctggcgagca gcatcccggc cggctcgccg tcgagcgcgc cgccggtgcg ctggtagagc
     1561 gtgcgcgcga gctcgtattt cttggcgcgg gcggcgatca tcgcctggag gtagaaggcc
     1621 atcgggtggt tttccgacag cttcagcacc ttccgcgtct cggccagcat cgccttggtg
     1681 tcgccgaggt cgccgagcgt ggcggcgcgc tcgagcatgg cggggatgtt gctggggtcg
     1741 atctccagcg cccggtcgaa ccagggcagc gccgcccgaa ggccatattg gctgcgggta
     1801 agctcgccgc gcagggtcag cgcctcgatg tccttggcgt tgatcttgag cgcgcggtcg
     1861 gccgcctcta cggcgccggc aaggtcgccg ctgccgcggc ggaagcgcgc gacttcggtc
     1921 caggcggcat tgtccttcgg gtcggcggcg atcgcttcct tgaaggcggc ggcggcgccg
     1981 gcattgtcgc cgagcttggc cagcgcctgg ccgcgcaggc gggcggcgta gccggcatga
     2041 gcggcgggga cgccgttcag ctcctcgagc gcgcgcttgg gctcaccccg gagcagcagc
     2101 gcatgggcga gcaggtggcg ggtgtcgtcg accgcgacgc cgagcttgcg ggcgcgctcg
     2161 atctccgcct cggcggcgac gccgtcgttc agcgacaaat aagtccgcgc ctgcgcgacg
     2221 cggatggcgc cgttattggg atcggcctgg atcgcgttca tcagctcgac ccgcgccgtg
     2281 cgcggctggc cctgctccag cgcggcgagg ccgcgggcgt agctatcctt ggcggacccg
     2341 ccgccgccgg gcgcgttcga acaggcggcg gcgcccagca ggagcgcgca ggcgaagata
     2401 tgccgaggct tggtcatggc tggagtccat attgcttgag gaggtcgtaa agcgtcgggc
     2461 ggctgatccc gagcagcttg gcggtgttgg tgatgttgct ttcggtgcgg gcgagcgcct
     2521 ggcggatcgc cttgcggtcg gcttcctcgc ggaccgcctt caagttgacc ggcagcggct
     2581 ggtcctcgcc ttcgccgagg tcgagatcct cggccgtgat cagcttgcct tcggccatga
     2641 tgaccgcgcg cttcatccgg ttctcgagct cgcggacgtt gcccggccag ggccagccgt
     2701 tgatcgcggt cagcgcgctc ggcgcgaagc ccttcaccgc cgggttcatc tcctgggaga
     2761 agcggttgag gaaatgcttg gcgagcaggg tcgcgtcgcc gtgccgctcg gccagggtcg
     2821 ggatgcggat cacgatctcg gccagccggt aatagagatc ctcgcggaag ctgcccgcct
     2881 tgatcatctc gggcaaatcc tggtgggtcg cgcagacgat gcgggtctcg accgggatcg
     2941 gcttgcgccc gccgatccgc tcgatcaccc gctcctgcag gaagcgcagc agcttgacct
     3001 ggagcggcag cgggatgtcg ccgacctcgt cgaggaacag ggtgccgcca tgggcgagct
     3061 cgatcttgcc ctcggtggtc ttgatcgcgc cggtgaaggc gcctttctcg tatccgaaca
     3121 attcggcttc gagcaaattc tcggggatcg ccgcgcaatt gatcgcgacg aacgcgccgg
     3181 cgcggcggcc gctcgactgg tggaggccct tggcgagcag ctccttgccg gtgccgctcg
     3241 cgccgagcag catcaccgac acgccggcat gggcgacccg ctcgatcgtg cgggcgacct
     3301 tcagcatctc gggcgccgcg gtgatcatgg tgccgagcac ggtgcggtcc tcgccgatct
     3361 gctcttcgag ccggcgattc tcattctcga tgccgtggag ctggaaggcg cggcggacga
     3421 tcaggccgag ctggtcgata tcgaccggct tctggtagaa atcataggcg ccggacgaga
     3481 tcgcccgaag cgcgctttcg cgcgcgccgt ggccggaggc gacgatcacc ttggtgtcgg
     3541 gcttcagcga caggatcttt tccagcgtcg cgaagccctc gctgacgccg tccgggtcgg
     3601 gcggcaggcc gaggtcgagc gtgacgacgg cgggctcctg cgatcgcagc aggtcgaccg
     3661 cggcgttacg gtcggaggcg accagcacct cgtaatcgtc ataggcccag cgcagctggc
     3721 gctgcagccc ttcgtcatcc tcgacgatca gcagcttggc cttggccacg caattcttca
     3781 ggggggcgtt catgcgcgca tccgttcata gacaggggag gattcgctct ccgcggcggg
     3841 taggaagatg gtgaagcggc tgccttcgcc ttcgcggctc tcgacttcga cccggccgcc
     3901 catggcggtg atcagcgagc gcgcttcgaa cgcgccgatg ccgaagccgc cgtccttggt
     3961 ggaggcgaag ggctggaaca ggcgatggct gacgaagtcg ggcgacatgc cggcgccgct
     4021 gtcgatcact tcgacgacga tctcgctgcc gcgggtcccg cagcagaccc gcaccggctc
     4081 gtcggcgccg ctcgcgtcga tcgcattctg gacgaggtgg gcgagcgcct gttcgagctg
     4141 gcccggatcg gccagggccg agaggccggg ctcgccgaac agctcgaccg ggtgcccgcg
     4201 cttcttgatc ccgactaggc cggcgaggat cgcatggaga tcgacggcgc ttatcctcgg
     4261 cggctccgaa tggttgcccg ggctgagccg ggcgagcagg tcgttcatct tcttcaccga
     4321 gctttgcaaa gtggcgatca tgtcggcccg gaattcggga ttttcggcgt ggcgctcggc
     4381 gttgcgcgcg accaggctca gctggctgac cagattcttg atgtcgtgga ggatgaaggc
     4441 gaagcggcgg ttgaattcgt cgaaccgctc ggcattggcg agcgcgtcct ggctgcgcgc
     4501 ctcggcgagg tagctcgcgg cctggatccc ggcggtgcga aacaggtcga aatcctccca
     4561 gtcgagcggc ctgcgcacca gcggatgctc gagcacgacc agcccgacca ggcggtcgtt
     4621 atgcagcagc ggtacgccgg cccaggccgc accggccggg tcgatccagg ccggaatgtc
     4681 gacatgctcg cgcttgcccg atttcaggcg cccgccggag ccgtggaagt cgatcacgtg
     4741 gttggtggtt tcgaggaagc ggatgaaatg atgatcgctc gcctcgcgct ggccgaaatc
     4801 gtggtgccag ttccagcgcg cccccgcggt cagccggtgg cggtcgtcga gcaggagcag
     4861 cagtccgccg ggcgagccgg aaatgtcggc cagcgccttg atcacgcggt cgccgagcgg
     4921 cgccgcctgg cttccgccaa ggccgacggt gcgggtgaag cgcagccatt cctggcggta
     4981 atcgtagcga tgctcgaaga aatgcttagc gaggatcacc cgcagccagg cgcgcgcccg
     5041 gcccgacggc aggaagatga gcgccgccag cgtcatgccg aagatgatgc cgatctggcc
     5101 gatccgcacc cactcgccgc cgatcacttc caaggcctgc gtcgccgaca tcatcaggat
     5161 cagataggcg aggatcgcca tcaccgagat cgactggaag gtcgcggcgc gcgaaatctg
     5221 catgcgccat tgcgagttgc gcttgctcgc cagcgtgaac aaagggacca gcatggcgat
     5281 gatcgcgccg cgcatcgcga acagatcgtc gaccggcccg cgggtgaggt aggcaaccgt
     5341 gtagagatgg aggtcgtagg tccacatgcc ggccagcgcg agcatcggca gccggatccc
     5401 ccagcgcgaa tcgggcgagg cctggccgta gagattgtgg accagcacca gcgagccggc
     5461 ggcggtggtg aggccgagaa tataagcggt ggcggtcagc gcctcgtgga cgatcgggtt
     5521 gccctcgaag cgcggcagca ctccggcgac cacgatctgc aggccgatca ccgcggcgac
     5581 ggtgaaatag acgagcttca ccgcccgctg ccgctcgtcc tcggcggcgc tccggacgat
     5641 gccgtgcatg aaggcgaggt aagcgaagtt gcgcgcgcat tccgacaggc cggagagcag
     5701 atgatgcggg ccgagcaggg caacgaagat cgcccagacg gcggtgaccg cgaacgcagc
     5761 gaccagcggc cggttgcgtt ggtcgccgtt ccagtggcga agctgccaga tggcgagcgc
     5821 gccgaacagc aaggcggcga gcgcatggct ccacaggccg ataaacccga tcatcagcgc
     5881 gcgccctccg gccagaggac cacgcggatg gtcaggagca ggatcaggat gtcgaggaac
     5941 ggcgtgtaat tcttcgcgta ataaaggtcg tattcgagct tctggcgggc gtcctcgacc
     6001 gaggcgccat aagggaaatt gatctgcgcc cagccggtaa tgccgggctt caccatgtgg
     6061 cgctcggcat aataaggcag gcgcgcctcg aggtcggcga cgaattgcgg ccgctcgggc
     6121 cgcgggccga cgaagctcat ctcgcccttc agcaccgacc aggcctgcgg cagctcgtcg
     6181 atgcgcagct tgcggatgat ggcgcccacg cgcgtcaccc ggggatcgtc cttctgcgcc
     6241 cagaccgcga ccccgccgac ctcggcgtcc tgccgcatcg aacgcagctt caatatatcg
     6301 aagccctggc cgtacagccc gacgcggcgc tggcggaaga aggccggccc cgggctttcc
     6361 agcttcacca gcaaggcggt gagcaggatg gcgggtccgg tgatgatcag gatggcggcg
     6421 ctcaccaata tgtcgaacag gcgcttgccc gcgctcgaca gccgccgccc cgaggagaag
     6481 ccgtcggaga agatcagcca ggacggattg aggctgtcga ggtcgacgcg gccggtctcg
     6541 cgctcgagga aggaggagag gtcgttgacg tggacgccgg tggtcttgac ccgcagcagg
     6601 tcggcgagcg gcagcgcgtt gcgccgctcc tcgatggcga gcacgacttc gctggcgcgc
     6661 agccgcacga cgaagtcgga caggttggag atggcgtcgc ggttgacggc atgggcgacc
     6721 gcggccggcc cgtcgttcat cgcgacatag ccggcgatgg tgaagccggc gccgtcgcgg
     6781 cgcgcgagcg cttcgagcct ggcggcgcgc gggccggcgc cgagcaccag gatgcgccgc
     6841 ttgaacgcct cgctaccgag cgagcggccg agcgcgatcc ggatcccgat cagccccgcg
     6901 atcgcgaaca gcatcgcgta gagcaggttg gagcgccaga aggtgagcgg cggcgcgacg
     6961 aagaagacca gcgacaggag cagcgccccg agcgacaccg cgaccagcag ccgcggcgcc
     7021 gcatggcgca gcgactggag cgattcgacc gcgtagacgc cgaccccggt catcgcgagc
     7081 tggagcgtga tcgcgaaggt cagcatgttc ggcagccgcc ccggatcgag ggcgatcttg
     7141 ctgccgatct gccagtcgcg cagcagccat ccggcctcgg ccgccagcat cagcgtgacg
     7201 aagtcgatga ggccgagcag cagcacggtg tgcggcacat aatgtttgaa caggcggatc
     7261 atcggcgccg ctccggccgc ggcatgcgcg cggcggcggc agggcgcccg gcgcctgcaa
     7321 gtgtaagctt ctccgacacg ggcagcgctg tcgcagaaat tgacgaattt tctctaaagg
     7381 cgcgcaaagg aaccggtttc cgggggcctc ggatagaatg tccagagcgc ccggatcaac
     7441 ccggcacgat tgccaccgtt cggccgcctc ggcggcgctc ttccgctttc gagccattgc
     7501 ggaggctggc agcggtcggc agactgctcg cgcttgacag ggagtggccg gacgatgcga
     7561 cggagagaga ttctggcagg agcagctttg gccggcatgt tcgcctccgc gccggccttc
     7621 gcttgccgtt cacccaagcc aaaggaccag gccggctacc tgaaagttat caatggcctg
     7681 ttcgccgcgt ggttcgctcg cgactatccg gaatttcgca agttctttct gcacccggaa
     7741 ctggacgagc cgttcgcgag ccagaacata ttccacgaac attatgatga cgagcgaccc
     7801 cgccaacgcg gttccttgat gttcaacggg ccatcggcaa ttgtgcaggt catcgcggtt
     7861 cagccagcgg actcatggtc atgtggaggc cacggttggg cggaactgat gctggtgaag
     7921 ttctttctgg ggatcgaggc gccggtggtg gccgaactcc gttaccttga cggcgcgaca
     7981 ctcgctaagg gggaatggac gccgcagtcg ccgggagcgg cgaaacgata gcgtccactt
     8041 tccatccagt gcggcgccgt ccctgccagg ctggcgcgaa ggtccgccgt gaccgattgc
     8101 ggacattccg gaacctggtc gagcagcggc ccacaggagt gaatcgcccg atggatagcg
     8161 gattgatgtt cctgttcgtg cccgagatgt tcctgctcgg gctcgcactc tctttcgccc
     8221 tccatctggc gctggagaga cgctagctgg ccgccatcgc gctcagcgcg gcgttgccgc
     8281 cggcaaggca gctcgtggcg gcgcttgttg ccggcggcgc acccgcactg gatgccgaaa
     8341 ttatgttctc gctagcaatg ctcgggggtc tcggggtgct catcgccgct ctgggcgcgg
     8401 tgtttggctg tcctgtcggt ttggcaatag gctccctgtt ccgtcgctga agcggcggga
     8461 tgttcagccg cgaagctccg aaaggtagcc gctgagggcg ttcaccacct cgccagcgcg
     8521 cgggcgaacc cccggggcgt cgccaagccc catatctcga gtttcgacgc tcaccagata
     8581 gtcggcgacg tcctcttcgc tcgcgccgtt ccacaggcgt ccagcggctt cgagcagata
     8641 gctgtcatat tcatcgtcgg gagtgctttc catgccgctg aggccgatcg gatcccaccg
     8701 gctccacccg atatcccgaa ggcgcgacag cttgaagggc gggcgagtgg tcatggttcg
     8761 gcctatcgcg gcgaaggtcc gctttccacc cattgccgag attcagctgc tgttgggctg
     8821 cggggcgata acggaagctg cgagggcttc tttctgatcg gccgcagcag cgacttcggg
     8881 cgttgcgccg aattctccag tcggttcgag cagcgtcatg accggttttc tcaggacgag
     8941 gacgagcaac aatgccgcgg cggccaatgc accggtggct gtccagtaaa gcggcgcgcc
     9001 caacggctcg tagaaccggc ccagccaccc gaggaggaaa taggagaaca tggtgctggc
     9061 cttgaggagc gccatcatcg tcgcgttgac cgaggcgggc gaatcccgcg aaaccagcga
     9121 ctgcatgggc ggctcgatcc agccgaagtt gaaatcgagg atgatgaaga aagcgatcca
     9181 aagcgcgacg ggaacgagcg gcagcgtggc gattgctccc gcgaacagga acgcaattgc
     9241 gataccggca taaccgatcg cgatcttggt caggtcgcgg ggctcgcggc cgcgccgggc
     9301 cagcctgatc cagatccggt tggcaatgac cacgccgagg atcgtcaaca ggccatcgac
     9361 cactccaatc cacgtaaccg ggacctccca gccgagcacg acccgatcga cggccgtgtc
     9421 ggcccaaacg aacatgatcc catacgcctg catggcagcc gcggtgtaaa gcatgaaggg
     9481 tattagcagg cacaggaccg cgcctacgcg gcgccactcg ctgcgcgtca gccttgtcct
     9541 ctccgcgcgc gcggtgacgc ggtccggcgg caggtgccgg cgcccggcca gataggtcac
     9601 caggccgatc accatgccga tgcccgccgc accgaagccg taatgccacc cgaccttctc
     9661 tcccagggtg ccgatcacca gcggcgcggc gagcgctccc atattgagcg tgatcaggta
     9721 aacgccgaag gcgcgcgtac ggcgattatc gtccggtgcg tagagcagcc cgacctgagc
     9781 cgccatgttg ccgatcactc cgcccgcgcc gaggatgatc agcatcaagg caatcaggaa
     9841 cagatgctcg aatgccatag ccaaatggcc tgccgccatc agcaggcagc cgacaataat
     9901 tgtccgggtc ttgccgaaga cccggtcgcc cagccacgct cccagcagcg gagtgccgta
     9961 gagcagcccc gaatagatgc cgaaagtctg ggcggcgaag gcgaggtcgg tcatcggacc
    10021 cgtgaggtcg acgagggccg agcggaatgc gccgagcccc agcacttcgt cggcacgctg
    10081 cgggaggagc agatactgcg tcatgtaaag catgaggagc gcctgcattc cgtagaatga
    10141 gaagcgctcc cacatctcgg tcgatgccag gaccgtcagg ccgcgaggat ggccgaacat
    10201 ttcgctgcgt tgttcgtgac tcccggcgct cgctgccatg ggcgcctccc cggttcacat
    10261 gcctacgccg gcggttcggc tcacactagt tatttcgtct tccgacccat agcggacgtt
    10321 caccaagtcc tggcgtggag gtagccggag aggttccgga gaggcgtgcc ccaatagcct
    10381 cgaaaccaaa gcttgaagtg ccaatgggca tcgtcgggtc cgatgttttc atgcctccag
    10441 accacctcat caaggtcctc ggcgacgtcg gcgagatcgt aaatggagtc cccgatccga
    10501 cctgtctcat gcaaaggccc gacggggtcg gtcagagcat agaagccata ttcggggaac
    10561 ctctgcccca agcgcgcata acgcacctgg aaatcctcta tcggcggctc cctctcatcg
    10621 tcggcgacag acccttcggg tgcctcatga tacgccattg agagctcatc cagggccctc
    10681 gccagctcct cgtcggatgg cgatcgccct caccgatgaa ggccaggaaa cggcgcaccg
    10741 aagcgattgt gggagtgtcc tgcatcggta tggcgctgcc acgcggaccc ccggtgttca
    10801 acctgtgctt ccggccgatg gcggaccttc aggggtcccg gtcccaaaag cgggtgggaa
    10861 gcggcgcagg ctccgatcct tccgccgggt cgcgccgaat gtcgatgaca tggcgcgcgg
    10921 agaatggaag ctcgacgccg ctccaaagct cctcattctc gccgatggcg cgaggatcgt
    10981 tatcgaggat gccgagatac tggtcgccca cccgttcgcg aacgatcacc cacatgcgtt
    11041 cgaccgcgac cggctcctcg tcattgtcca ccgctaggcg gaagatcagc ttcacgaggt
    11101 ctcccggctg gagcgattgc cggtccgtca attcgggaag ccagaatgtg gccggagctt
    11161 cgcgccggcg ctcctcgcca tcctcgaggc accacccgtc ccgctcgaaa tctgctgctc
    11221 tcattcgcga accctaagtc gggcaacgtc cgctttccac ccgttgcggc gatcccgatc
    11281 atcggtcatt ctgagaacat ggccatacgc gcgagcatcc tcatcctggc cgcagcgatg
    11341 gcgggttgtg catcgggcgc ggcactggtc gcggtgcccg atgattggca ggctgaggct
    11401 cggcgggagc acctccgcaa caatcgaacg atgatcgagt cggaagccgc ggcgtgtagc
    11461 gctcgcgggg gcaagctcgg ctaccgcggg ctggcgctgg cgccattctg cacgatgcgc
    11521 cattccgacg gcgggaaggc atgccggtcg caatcggaat gcgacggaga atgtatcgtc
    11581 gatatcgaag gtaaaccggc tgccatgcga agcttgagcc ccggagcggc ggcagtcggt
    11641 cgatgcacgg cggcctcacc gcatctgggc tgctatgtcc cggtcgacgg gggtgaagcg
    11701 ggtcaagcca tgtgtgcgga ctaacgttcg ctttccatcc cgtggcggag gctgggctga
    11761 cttcgtcggg gtcgtgccag tcgcttgaat ggccgcttcc agcgatagcg gaccgccaac
    11821 ggcgcgaagc tcatgaccgg gccggcgggc ctcctgcggc tatggtctcg ggctcgaggt
    11881 cggggcctcg gtcggtgcag gcgggctttc cttcgaagcg gcattcattg gtgatcgtgc
    11941 ctgtgatcac gacataaccg tgggtgaagg gcgcggcctt cgcatcgaac tcgaagttgg
    12001 tcccgctccc aattccgagc gccggaacgt ccgggatcgc gacccgccgg ttggcgctca
    12061 gagcgacctt gacccgatcc cattggtggg aatcggcctt gctccgatag aggatgcaat
    12121 tattcccgcc gcactcgggc aggtacccgg cgacgctcac cgtccggccg tcaagctcat
    12181 cgatccgggc gacaacctcc tccaccgtca gaatcttccg cggcgccgcc ggaccgcagg
    12241 acgtgccgat gatcgccgca agcaggatcg ccgccgatcg cacgcgggat cgcttggtca
    12301 ttccagcagc ctgactgccg cgatgtccgc tttccaccgt tgcggacatc aattcagcaa
    12361 gcagtccgaa ggcgggagcc gcagtgcttc tcgctggcgg gcgacaagtg cggcaatctc
    12421 gtcgcgaatg gcagatggtt ggttggcgag ccgagccata tgtcgtgcca ttcggcccag
    12481 cacgttcggc tccccgcctg agcttctaag aacgaggccg aacatctccg ccgcgtcgtc
    12541 tccacgtccc gcgagcgctg ccgcgacacc ggcgtagtag gccatccatg agccgtggcc
    12601 cggcgggtgc ttgcttcgca cttcatcgag gagcacgtcc gccgccgcgc tgcagaggtg
    12661 aaggtctgcg ccaggcgctg cgcttcctgc gccgccatct ccgccaggcg agcggcggcg
    12721 ggggtgaact gatcgtccga aacatagtcc acgaactcgg atgcgcgatc gccgaagtcg
    12781 aagctcagca cgcccactcc ggaccagagc caatgagcgg cgacgttgag atagcttccc
    12841 ttgctccacg cgctcggctg aaactcgacg atcgtcagcc accatccatg atctgccaac
    12901 catgtgcggg aacgcccctt gcgttgaaag ccgagcgggc gaagcgaagc cttggctgcc
    12961 tcagcgatga tcctgtcatg cggccctggc atccgatccc cctgccgcag cctatgccgg
    13021 ccggcgtccg ctttctaccc ggggcggaca ccaattcctc cccagtgggc gtggccgcaa
    13081 cggggaggct cgtgagcgcg cggcgttgcg gacgcctcaa tccgcggcca tggtctgccc
    13141 aggaggtagc gaacggaaat gaggatcgtc ggcagttatg tgagccccta tgcgcggaag
    13201 gtgctggcct gcctcgcgct caaaagcgtg gcctatgaga tcgatccgat cacgcccttc
    13261 tacggcaatg acgaattcga gcggctgagc ccgttgcggc ggatcccgct gctgatcgac
    13321 ggcgatttcg tgctcggtga ttcctcggtg atctgcgcct atctcgacga ggcctatccc
    13381 ggtccgccgc tgctgccggc cgatgcccgc gagcgggcgc gggcgcggtg gatcgaggaa
    13441 tatgccgaca caaggctcgg cgacatcttc atctggggcc tgttctacca gaagatcgtc
    13501 catccgatcg tgtggggcga gcccggcgat gccgcccgga tcgagcgtac gctgagcgtc
    13561 gatgcgcccg ccgcgtgtga ctggctcgag cggcagctgg gcgaacgcgc agtggcggac
    13621 gggttcctgt tcggcgccga catcggcctt gccgacattg cgatcgccag cttcttccgc
    13681 aatgccgctt atgccggctt cgcgcccgat ccggccggct ggccgttgct cgccggcttc
    13741 ctggagcgcg cgctcgccca cgactgtttc gcttgcctgc tcccgttcga gcaggcccag
    13801 ctcagcacca acccgcaggg ccggcgcgcg gccttgctgg cggccggagc gccgctgtcg
    13861 gcccagacga tggggacgcg ggtgccgcgg cgcggcgtga tgcggctctg aggccctgag
    13921 cggcagccac ctttgcagcc actttcagcc cgcgcgcgtt gaggccccat gtctgctttt
    13981 gccgctttgg ccatgatgac ggtggcgccg ccctgcgcgc ccggcccgct cactccgaac
    14041 gctccgatcc cgccgcccga gcgccgcgtc gacggccgct cctccgcccc ctatggcgat
    14101 gcgttgggcg atgcctggga aatggaggag gtcgcctgct ggcgcggcat ctggcagcgc
    14161 cgcggcgagg gccggctgtg ggacggttac tggttccacc cgggcggcga gcgggtccgc
    14221 gcgacgctcg aaatgtggcg cgacggccgc tcggtgatcg tcgtccgccg acaccctggc
    14281 ggccaatatt gccgctacga cggcacgatc agcgccgatt ggtggaccat cgaaggccgc
    14341 tacacctgca cctgggagcg cacgccgatg ccgtggcgcg ccgagatcgt gcggctcgaa
    14401 catgcggagc cggccttgct gcggcgctga ggcctgaggg cccggaggcg cagcttcatc
    14461 gcgggcgcgg ccgccttcgt cgcgggcgcg gcggcccggt cgcggcggac cgcatccgcg
    14521 tcgaaaccgg ctccatcctc gacgcaacgc ccggcattgg gcggccgatc gaccggcgaa
    14581 ggagccccgc ccacatgttc gagaccaaga tcgaccgcga ccacgcctgg ccgcagaaga
    14641 cccgcgagct gcacagccac catttcgatt ccaccatctg gaacgacttc cgcttccgcg
    14701 acgacgacat cgtcatcggc acctatgcca agtccggcac cacctggacc cagcagatcg
    14761 tcgcccagct cgtcttcaac ggcgccgagg gcatcaacgt cggcgaaatg tcgccctgga
    14821 tggacctccg cctgccgccc aaggaagtga agctgccgct ggtcgaggcg cagacccatc
    14881 gccgtttcct caagacccat ttgccggtcg acgcgctcgt cttctcgccc gacgccaaat
    14941 atatctatat cggccgcgac ggccgcgacg tcgtctggag catgtacaac catcactcca
    15001 acttcgtccc cgaaacctat caggcgctca acgacacgcc cggccgggtc gggccgccgc
    15061 tcggcctgcc gccggcgtcg atccgcgact atttcaacga gtgggtggag cgcgacggct
    15121 accccttctg gtcgttctgg gagaatatcc gcagctggtg ggagatccgc cacctgccca
    15181 acgtgatgct gctccatttt tccgagctca aggccgacat gcccggccag atccgccgca
    15241 tcgccgattt cctcgagatc gcggtcgacg aggcgagatg gccggcgatc ctcgaccatt
    15301 gcagcttcga ctatatgaag cgcaacgccg agctggcggc gccgctcggc ggcgtgctgt
    15361 ggcaaggcgg cgcgcagacc ttcatccaca agggcgtcaa cgggcgctgg cgcgacgtgc
    15421 tcagcgccga cgattgcgcg cgctacgaag cgatggcggc cgagcagctc ggcgccgatt
    15481 gcgcccgctg gctggcgagc ggcgctcaca cgggctgacc gccccggccg ctgattcggg
    15541 cggcgggagc ggaatggaag agtggatcgg cgggaccggc gtgtccggtg aggccgagct
    15601 agcgccttgc cgcggcggcc gaagctgata acctgcccgc ctccgccact cgagttcgct
    15661 tatgtccgcc gccggccctg tttccgcccg cttcgccggg atcggcccgc gcaacccgcg
    15721 gacgcgcggc gcgatcgcgg tgtgcctctt attctacgcc tttaccttga tggtgttcac
    15781 cgtcgccggc ttcttccgcg gtcctgaaag cttccgcgaa tccctgtcga tcggcgtacc
    15841 gatgatgatc gtcgacggct tcctgagctt ccagatctac ttcctgctgg gccccgcatc
    15901 gcggctgccg gcggtgccgc gctggtcgct gatcgtggcg ggcgtggtgg cgatcgcgct
    15961 cctacaggcc gtgtgggaca cgcagctgcg gctgtgggcg gggtcggtgg accaggccgg
    16021 cgcctatctc gcttatgtcc ggtcggcgac gctcaacgtc tacaataccg gcatgttcgc
    16081 cgcgttgatc gccttccagg gcgcctatct cgacctcagc gagcatcagc ggctgctcaa
    16141 cgcctcgcgc gagagcgagc gcgacgcgca catgctggcg ctgcgcttcc agctcaaccc
    16201 gcatttcctg ttcaacaccc tgaacgccat ttcgagcctg gtgatcgtcg gccgcgccgc
    16261 ccatgccgaa gcgatgatcg accggctgtc ctcgttcctc agggccagcc tcaccgccga
    16321 tccgcacagc ctggtcagca tcgacgagga attcgagatg ctcgattcct atctcgagat
    16381 cgaaagcgtc cgcttcggcg accggctggt gaccgcgatc gacctgccgc cgccgctgac
    16441 ccgcgccttg atgccgcctt tcctgctcca gcctctggtc gagaatgcga tcaaatatgc
    16501 ggtcgcgccc tccacccgcc cggtcgaggt ccggatcgcg gccgaggagc aggacgggcg
    16561 gctggtgctg accgtcgagg acgatggcga cggctgcggc aacggcagcc cgggcaccgg
    16621 cgtcggcctc gccaatgtgc gcgagcggct gcggctcaac tacggcagcc gggcgcggct
    16681 cgaaatgcag caggacggct ccggctgccg ggtacggatc gtgctgccgc tcgagcttcc
    16741 ggacgagagc gtggcggccg cgcggcccgc cgacgtcttc gcctgagcca ccccttccgc
    16801 ccgccgtcgc cccggtgtag catcgccgca atacacattg ggaggccgca cttggtcaga
    16861 tcgtcacgct tgttcctggc ctgcgccgcg ctcgcgctag ccgcgcccgc ctgggccgcg
    16921 ccc
//
LOCUS       DASVRK010000184        10550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_222410, whole genome shotgun sequence.
ACCESSION   DASVRK010000184 DASVRK010000000
VERSION     DASVRK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222410"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VF589_07230"
     CDS             <1..1485
                     /locus_tag="VF589_07230"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX8257408.1"
                     /translation="CGGGSSGRVDGGLPLSPTLSPEGERGQNVTRPDHAAGAEKARAT
                     AAVDRALAGFSAWSMQSIEHRAACLDRLADLLERERDALMGLAVQEAYKTIPDALGEV
                     REAVDFCRYYAAQARSGLQPIELPGPTGERNVIRMEGRGAWVCIAPWNFPLAIFLGQV
                     TAALVAGNTVVAKPAPQTPQIAAYAVGLAHSAGVPEDALVLAVGGPETGQALMEDVRI
                     AGVAFTGSTATAKRIARTLLIDDERPIIPLIAETGGINAMIVDSTALPEQVVADVVTS
                     SFRSAGQRCSALRVLLLQEEIADRTLEMLAGAMDTLVVGDPADPKTDVGPVIDQGAYD
                     RLMAHRHAKQNSWLKTVPVPEQGLFVPPTMIGVPEIENVRCEHFGPLLHVTTWKAGKL
                     GDTIARVNATGFGLTMGLHSRIARSAEAVEDVARVGNLYVNRSMIGAIVGSQPFGGEG
                     LSGTGPKAGGPHYLPRFCAERVTSTDTTSSGGNATLLSLEDVGL"
     gene            complement(1620..2492)
                     /locus_tag="VF589_07235"
     CDS             complement(1620..2492)
                     /locus_tag="VF589_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257409.1"
                     /translation="MRKTAAAIAAAILCGGGVPAPAAAAAPPAAPQCPPLAGAAEVLA
                     LTGKRYILFGEIRGTVEAPALFGDLVCVAASAGPVIVSLDLEEGQQAPLEAYLSSDGS
                     PAARAALTGTRHWSTAVDGTASGAMLALVEQLRLLKKRGLPLSLITSRRNPGGTGSAA
                     SKAMADIWMASLKARPGARLLALVGNFQAMRKPLNGGVAAQVPPALAHPPAAAHLPAA
                     ETLSLNAASVGGFAWHCQQECKVHDLRERPRKIHPRAILPIPVEDPDSRQFDRWYSPG
                     AAFTASPPVSVKEL"
     gene            complement(2528..3706)
                     /locus_tag="VF589_07240"
     CDS             complement(2528..3706)
                     /locus_tag="VF589_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016510712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8257410.1"
                     /translation="MSQANQANNASVTDAAQNLSEKSVRIASEASAWLTGNSVDILIA
                     AAAGAVIALLLLGIRGFGHRLVHDRSGAGIKWRTLVGRVLARTTLFFIVMCSAQLVAE
                     HAATPAALLRPINVLFVVAAAFQAAIWARELILGLVQHKVGEEDSHSTLGSAIGIIRL
                     LVTVALFAIAIILILDNIGVNVTGLVAGLGIGGIAIGLAAQGIFSDLFAALSIIFDKP
                     FRKGDGIKVDQLQGTVEEIGLKTTRIRSLSGEEIIISNAKLLDLQLHNFSNLEHRRFT
                     LMLGVVYQTPPEVCARIPEIVKGIVEAHPPASLVRCGMAGFGASSLDFELQFDVHTSD
                     YELAFATRAAICLDILATFSRERIEFAYPTQTTFTAAPDGTLVMPYPHVKMVATEDLK
                     "
     gene            complement(3753..4856)
                     /locus_tag="VF589_07245"
     CDS             complement(3753..4856)
                     /locus_tag="VF589_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX8257411.1"
                     /translation="MMLRYVEDQGPGITRKRRGRYWMYFGPDGERIADREEIDRLNAI
                     GMPPAYERCWFCPDPNGHMQAVGYDAKGRKQYRYHPEFRAEQESVKYERLAAFGRALP
                     KLRKRVETDMDGRSMRRDTVLAAVVRLIDTTHMRVGSEEYAQANKSFGATTLRNRHAK
                     VKGNKLVISYAGKHGVKRSITIHDRRLIRIARRTQELPGQHLFEFVNEDGGVCPVTST
                     DVNAYIKEAMGDDFSAKDFRTWGASVIAFEEIVRRVETASRIRLKNVIEPVAEALGNT
                     PAISRKSYVHPALIEALKDSGAIGGRKLPRKTAHLSAYERGLIEFLEALPKEEKKLDD
                     AAEAVARTERKSRAAAKAEVVAAAKARLEAEAV"
     gene            4962..5510
                     /locus_tag="VF589_07250"
     CDS             4962..5510
                     /locus_tag="VF589_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX8257412.1"
                     /translation="MPAIANARILFIATDGFEDSELLDPLNVLREQGATVTLASLEHD
                     EIEGDKGARVKPDALIGDIDSKDFDALVLPGGTKNPDKLRMEEQVVYTVRKFVDAGKP
                     VAAICHGPWLLAEADVIRGRTVTSWPSIRTDLRNAGANVVDREVAVDGNLVTSRKPDD
                     IPAFIQAFIGLVEKAEAAMETA"
     gene            5934..6218
                     /locus_tag="VF589_07255"
     CDS             5934..6218
                     /locus_tag="VF589_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257413.1"
                     /translation="MQLLIILALAAAGSAVRVGPSAAELAAAIRASPVVSGPVTFRTD
                     TIGSVRCHRTEEEPTEYFCRFRARAKEGSWMRRSAILAMDGGRWILLSLD"
     gene            6304..7083
                     /locus_tag="VF589_07260"
     CDS             6304..7083
                     /locus_tag="VF589_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257414.1"
                     /translation="MWFQLPLIAALVALSGPVGAPQAAQSVAPGPGAAGVSDVIEGVE
                     PPEEMIDFLGRRQLCHELREEGPPRTDDRARVMREELERLACSSLPAEEKLLRSKFAA
                     DAASIAWLDGDPATFKIGRIVATLYHGPPPVRAHRIEQRGADHRGEASYHLLIDTRAE
                     GGRSTTVTAAFGDVPSRTITLSNARFPHIDPGSIFAAVETKGPRAGMLVTIRHGYLRG
                     YCGTVEEDDRPKIALTFAATEVTGSYTDMTNCRPDSVELRF"
     gene            complement(7113..8510)
                     /locus_tag="VF589_07265"
     CDS             complement(7113..8510)
                     /locus_tag="VF589_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HEX8257415.1"
                     /translation="MRDLAFVGFLLALIGFGFRRPFLFVLAYAYVDIVAPQRLSYFML
                     NAVPVSMIVAGLAIAGWLVADDKRDFRVTGRQLLMLILLGYAGYTTIYADFPVDALFK
                     WEWVWKAMMWAIFLPFTLRTRLRLEAYLLFMTLSAAAIIIVGGIKTAASGGGYGALNL
                     MVDNNSGLYEGSTISTVAIAVIPIILWLARFGTIYPRDWRVRVFAYNLIFACLLIPVG
                     TEARTGLVCIAALVMLMLRDVRNRIGYLALVAVALAVAVPFLPQTFTSRMSTIQSYEA
                     DASASTRIAVWGWTWEYVKDRPMGGGFEAYKQNSLRVNTVATKTAGAVQTVDQSQIQD
                     SARAYHSSYFEMLGEQGFPGLILFLLIHAIGLVRMEVLRRRFRRAEGDQAWIAPLATA
                     LQHAQLIYLIGGLFVGIAFQPFVYMLVAVQIGFDTYVGRKTREQAKAPWGRKPDGPQE
                     AAPVGAPVAAVGKAG"
     gene            complement(8503..8664)
                     /locus_tag="VF589_07270"
     CDS             complement(8503..8664)
                     /locus_tag="VF589_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257416.1"
                     /translation="MIDNFALGLTHGLLILAAWRLLTRADLDDDTIPAPTPPAPRDRR
                     IGAKERPGA"
     gene            8754..9956
                     /locus_tag="VF589_07275"
     CDS             8754..9956
                     /locus_tag="VF589_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04063 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HEX8257417.1"
                     /translation="MRILHVLDHGLPLHSGYTFRTRAIVKAQLARGWEVACLTGPRHS
                     APGPDPETIDGITFFRTPQPAPAPSPLREWREVGALADRLDRLVGEWRPDQLHAHSPV
                     LTALAALRVAKRRRLPLVYEIRAFWEDAAVGNGTGREGSARYRLTRALETWAARRADA
                     VAVICEGLRRDLIARGIAPERIIVAPNGVDMSLFGNPPAPDAALAARLGIEGDVVGFI
                     GSFYDYEGLDDLIAAMPLLLERRPAARLLLVGGGPMEERLKAQAAASPAAARIHFIGR
                     VPHEEVELYYSLIDVLAYPRKAMRLTELVTPLKPLEAMAQRKLVAASDVGGHRELIED
                     GVTGTLFRAGDPAAIAEALDRLFAGRAGWDQRREAARSFVEKDRNWSSNISRYIPAYQ
                     RLTGKVLA"
     gene            9953..>10550
                     /locus_tag="VF589_07280"
     CDS             9953..>10550
                     /locus_tag="VF589_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257418.1"
                     /translation="MTSNTMKRARKIAKLPLDLPMAALAAASVAFVAYAMPDDLFSRG
                     VGLTGLPSVLAAAEPPLGATARLAVIAAGAAGTFLIVWLLLRALGKPSAKARRLAEEE
                     AEMAPPRLRRADAHPDAPSRRPLIAGLDLGEPFDALPRGRQYREPQEEPEALTQWEPS
                     LPSFMQVEPDPREEEEAEELELQAEEAFEEAEPPVEVPS"
BASE COUNT         1644 a         3446 c         3782 g         1678 t
ORIGIN      
        1 tgtgggggag gctcgagcgg cagggtggat gggggactcc ccctctcccc gaccctctcc
       61 cctgaagggg agagggggca gaacgtcacc cgccccgatc atgccgccgg ggcggagaag
      121 gcgcgcgcaa cagccgccgt cgatcgcgcc ctcgccggct tttccgcctg gtcgatgcag
      181 agcatcgaac atcgcgccgc ctgcctcgac cggctcgccg acctcctgga gcgcgagcgc
      241 gacgccttga tggggctggc cgtgcaggag gcctacaaga ccatccccga cgcgctcggc
      301 gaggtccgcg aggcggtgga cttctgccgc tattatgccg cgcaggcccg cagcggcctt
      361 cagcctatcg agttgcccgg cccgaccggc gagcgcaacg tcatccgcat ggaagggcgc
      421 ggcgcctggg tgtgcatcgc gccctggaac ttcccgctcg ccatcttcct cggccaggtg
      481 acggcggccc tggtcgccgg caacacggtc gtcgccaagc ccgccccgca gacgccgcag
      541 atcgccgctt atgcggtcgg cctcgcccac agcgcgggcg tccccgaaga cgcgctggtg
      601 ctggccgtcg gcggccccga gaccgggcag gcgctgatgg aggacgtgcg catcgccggc
      661 gtcgccttca ccggatcgac cgccaccgcc aagcgcatcg cccgcaccct gctgatcgac
      721 gacgagcgcc cgatcatccc gctgatcgcc gagaccggcg gcatcaacgc gatgatcgtc
      781 gattcgaccg cgcttcccga gcaggtggtg gccgacgtcg tcacttcctc gttccgctcg
      841 gccggccagc gctgctcggc gctccgcgtc ctgctcctcc aggaggagat tgccgaccgc
      901 acgctggaga tgctggccgg ggcgatggac acgctggtgg tcggcgatcc ggcggacccc
      961 aagaccgacg tcggcccggt gatcgaccag ggcgcctacg accggctgat ggcgcaccgc
     1021 cacgccaagc agaatagctg gctgaagacg gttcccgtgc ccgagcaagg cctgttcgtg
     1081 ccgccgacga tgatcggcgt gcccgagatc gagaatgtcc gctgcgagca tttcgggcct
     1141 ttgctgcacg tcaccacctg gaaggcgggc aagctcggcg atacgatcgc gcgggtgaac
     1201 gcgaccggct tcggcctcac catgggcctc cacagccgca tcgcccggtc cgccgaggcg
     1261 gtcgaggacg tggcgagggt cggcaacctc tacgtcaacc gctcgatgat cggcgccatc
     1321 gtcggctcgc agccgttcgg cggcgagggc ctctccggca ccggccccaa ggccggcggc
     1381 ccgcattacc tgccgcgctt ctgcgccgag cgggtgacca gcaccgacac gaccagttcg
     1441 ggcggcaatg cgacgctttt gtcgctggag gatgtcgggc tgtaggcctc gtcgagcttc
     1501 gggccggcct cgtcgatcat cgctgtgatt acctccgcac cgtcaccatc cgaattggcg
     1561 ggccatgctc gccgacttcg aagcgcatcg gatggcggac gccgcctgat gtcccgaggc
     1621 tagagctcct tgaccgacac gggcggcgaa gcggtgaagg ctgcgcctgg agagtaccag
     1681 cggtcgaact gccggctgtc gggatcctct acggggatcg gcaggatagc gcgagggtgg
     1741 atcttgcggg gccgttcccg gaggtcgtga actttgcact cttgctggca gtgccaggcg
     1801 aaaccgccca ccgaggcggc gttcaggctg agcgtctccg cggccggcag atgcgccgcc
     1861 gccggcggat gcgctagtgc gggcggcacc tgcgctgcca cgccgccgtt gagcggtttc
     1921 cgcatcgcct ggaagttgcc gacgagcgcc agaagccgcg ctcccggacg cgccttgaga
     1981 ctggccatcc agatgtcggc catggccttg cttgctgcgg atcccgtccc gcccgggttg
     2041 cgacgggagg tgatcaggga gagcggcagg ccgcgcttct tcagaagcct cagctgctcc
     2101 accaaagcga gcatcgcccc gctcgcggtc ccgtcgacgg ccgtggacca gtgccgggtc
     2161 cccgtgagcg cggcgcgcgc ggcgggcgag ccgtcggagg agaggtaggc ttccagcggc
     2221 gcctgctgac cttcctccag gtcgaggctc acgatcaccg gcccggccga ggctgccacg
     2281 cagaccaaat cgccgaacag cgccggcgcc tcgacggtgc cgcgtatctc cccgaagagg
     2341 atgtatcgct tgccggtaag cgcgaggact tccgccgcgc cggcgagcgg cgggcattgg
     2401 ggagcggccg gaggcgctgc agccgctgcc ggagcgggta ctccgccgcc gcaaaggata
     2461 gcggcagcga tggcggcggc ggtcttgcgc atggtcgaag ctcctccgcg tcgattccct
     2521 tggccaatca tttcaaatcc tcggtcgcga ccatcttcac gtgcgggtag ggcatgacca
     2581 gcgtgccgtc cggcgcggcg gtgaaggtgg tctgggtggg gtaggcgaat tcgatgcgct
     2641 cgcggctgaa ggtcgcgagg atgtcgaggc agatggcggc gcgggtggcg aaggccagct
     2701 catagtcgga cgtgtggacg tcgaactgga gctcgaaatc gaggcttgag gcgccgaagc
     2761 cggccatgcc gcagcggacc aggctggccg gcggatgcgc ctccacgata cccttcacga
     2821 tctccgggat gcgcgcgcac acctccggcg gcgtctggta gaccacgccg agcatgagcg
     2881 tgaaccggcg atgctcgagg ttcgagaaat tgtggagctg gaggtcgagc agcttggcgt
     2941 tggagatgat tatctcctcg cccgagagcg agcggatgcg ggtggtcttg aggccgattt
     3001 cctcgaccgt cccctgaagc tggtcgacct tgatgccgtc gcccttgcgg aacggcttgt
     3061 cgaagatgat cgagagcgcg gcgaacaggt cggaaaagat gccctgggcg gcgaggccga
     3121 tggcgatgcc gccgatgccg aggccggcga cgaggccggt gacgttgacg ccgatattgt
     3181 cgaggatgag gatgatcgcg atcgcgaaca aagcgaccgt cacgagtagg cggatgatcc
     3241 cgatcgccga gccgagcgtg ctgtgcgaat cctcctcgcc gaccttgtgc tggacgaggc
     3301 cgaggatcag ctcgcgcgcc cagatcgcgg cctggaaggc ggcggcgacc acgaacagga
     3361 cgttgatcgg gcggagcagg gccgccggcg tcgcggcatg ttcggcaacc agttgcgccg
     3421 agcacatgac gatgaagaag agggtggtcc gcgccagcac ccggccgacc agcgtccgcc
     3481 acttgatgcc ggcgccggag cggtcgtgga ccaggcggtg gccgaagccg cggatgccga
     3541 gcagcagcaa ggcgatgacc gcgccggccg cggccgcgat caagatgtcg acgctgttgc
     3601 cggtcagcca ggcggaggcc tcgctggcga tgcgaacgct tttctcggac agattctggg
     3661 cggcgtcggt gacgctcgca ttattggctt gattggcttg gctcatggga ggcccatcta
     3721 gggacgcgcg gcccggcttt caaaacatgg cgtcagacgg cctcggcctc gagcctcgcc
     3781 ttggcggcgg cgacgacctc cgccttggcc gcggcgcggc tcttgcgctc ggtcctggcc
     3841 accgcctcgg ccgcgtcgtc cagcttcttc tcttccttgg gcagcgcttc caggaattcg
     3901 atcaggccgc gttcgtaggc gctgaggtgg gcggtcttgc gcggcagctt gcggccgccg
     3961 atcgcgcccg aatccttcag cgcttcgatg agcgcggggt ggacatagct cttgcggctg
     4021 atcgcgggcg tgttgccgag cgcctcggcg accggctcga tgacgttctt gagccggatg
     4081 cgcgaggcgg tttcgacgcg ccgcacgatc tcctcgaaag cgatcacgct ggcgccccag
     4141 gtgcggaaat ccttggcgct gaaatcgtcg cccatcgctt ccttgatgta ggcattgacg
     4201 tcggtggacg tgaccggaca gacgccgccg tcctcattga cgaactcgaa cagatgctgg
     4261 cccggcaatt cctgggtgcg gcgcgcgatc cggatcagcc gccggtcgtg gatggtgatg
     4321 ctgcgcttga cgccgtgctt gccggcatag ctgatgacca gcttgttgcc cttcaccttg
     4381 gcgtggcggt tgcgcaaagt ggtcgcgccg aagctcttgt tcgcctgggc atattcctcg
     4441 ctgccgacgc gcatatgggt ggtgtcgatc aggcggacga ccgcggccag cacggtgtcg
     4501 cggcgcatcg agcggccgtc catgtccgtc tcgacgcgct tgcgaagctt cggcagcgca
     4561 cggccgaagg cggccagccg ctcgtatttg acgctttcct gctcggcgcg gaattcggga
     4621 tggtagcgat attgcttgcg gcccttggcg tcgtagccga ccgcctgcat gtggccgttc
     4681 gggtccgggc agaaccagca gcgctcatag gccggcggca tgccgatcgc gttcagccgg
     4741 tcgatctcct cgcggtcggc gatgcgctcg ccgtcgggac cgaaatacat ccagtaacgg
     4801 ccgcggcgct tgcgcgtgat ccccgggccc tggtcctcga cataacggag catcatcatc
     4861 ctttcgtgac agcgcgttaa ttcctcgcgc cgccgctcgt tccggaacgg ccgcgcgggc
     4921 gtccggttga tctcaaggat attcacccga ggaggagctt catgccggcc atcgcgaacg
     4981 ccaggatcct gttcatcgcc accgacggat tcgaggattc ggagctgctc gatcccctga
     5041 acgtgctgcg cgagcagggc gcgacggtga cgctcgcctc gctcgagcat gatgagatcg
     5101 agggcgacaa gggcgcccgg gtgaagccgg acgcgctgat cggcgacatc gacagcaagg
     5161 atttcgacgc tttggtcctt cccggcggca ccaagaaccc ggacaagctg cggatggagg
     5221 agcaggtcgt ctatacggtc cgcaagttcg tcgatgcggg caagcccgtc gccgccatct
     5281 gccacggccc gtggctgctc gccgaagccg acgtgatccg cggccgcacc gtcaccagct
     5341 ggccgtcgat ccgcaccgac cttcggaatg ccggcgccaa tgtggtcgac cgcgaggtgg
     5401 cggtcgacgg caatctcgtc accagccgca agcccgacga catccccgcc ttcatccagg
     5461 ccttcatcgg cctggtcgag aaagcggaag cggcgatgga gaccgcctga gtcggcgatc
     5521 aggcgggctt tcgtaatgtc ggcatgaggc gccgcgccgg agcatcttgg ttcggcgcga
     5581 cggtcgatcg cgtcggccgg cggtaccgga acgcgctcca aaactgacga agttggggcg
     5641 tttgcaagag ccgtcgcgtc agttttgtca gtttacgcgg gcgggcgggc ggatcgtgag
     5701 ttcgtcccgg tgcaggccgg acggcgctcg gcacatggcc tgatacagta agtgcgatcc
     5761 tgcacgccgg tccctcagtc gcggccaatc ggccaggtta tggcggagga gggggctgta
     5821 ggaaaatgtt tgttcttgtt tcgcccctgg cggctgtcgg acggggaccc acgcctcgga
     5881 acggccgtgc gttgccgctt gccaactatc gcaacttgca ggaacgtcgg cccgtgcagc
     5941 tcctgatcat cctggccctt gccgcggcgg ggtccgccgt tcgcgtgggg ccgtcagcgg
     6001 ccgagctcgc cgccgcgatt cgcgcatcgc cagtggtgag cgggccggtg accttcagga
     6061 ccgacacgat cgggtcggtc cgctgccacc ggaccgagga agagccgacc gagtatttct
     6121 gccggttccg ggcgagggct aaagagggca gctggatgag gcgctccgcg atcctcgcga
     6181 tggacggggg taggtggatc ctcctgtcgc tggactgagc ggagcctcgc ggcccggaac
     6241 cggtcgagag cggggcttcg gcgacggctc gccagccagg gccatgtttg atagcctgcg
     6301 gtgatgtggt tccagctgcc ccttatcgcc gccctggtcg cgctgagcgg gcctgtcggc
     6361 gccccgcagg cggcgcaaag cgttgcgccc ggtcccggcg cggcaggggt ttccgacgtc
     6421 atcgagggag tggagcctcc ggaggagatg atcgatttcc tcggccgccg ccagctttgt
     6481 catgagcttc gagaggaggg gccgccccgc accgacgacc gtgcccgggt gatgagggag
     6541 gagctggagc ggctggcatg ctccagcctg cctgccgagg aaaagcttct gcggtcgaaa
     6601 ttcgccgccg acgccgcttc tatcgcctgg ctggacggcg acccggcgac cttcaaaatc
     6661 ggcaggatcg tcgcgacgct ttatcacgga ccgcctccgg ttcgggcgca ccgaatcgag
     6721 cagaggggag ccgatcatcg cggcgaagcg agctaccacc tgcttatcga cacgcgcgcc
     6781 gaagggggcc ggtcgacgac ggtgacagcg gctttcggcg acgtgccgtc ccgtacgatc
     6841 accttgtcga atgcgcgctt tccccacatc gatccgggct cgatctttgc ggcggtcgaa
     6901 acgaaaggac cgcgtgcggg aatgctcgtc acgatccgcc acggctatct tcgtggctat
     6961 tgcgggactg tcgaagagga tgaccggcca aagatcgcgc taacgttcgc ggccacggag
     7021 gtgacgggct cgtacaccga catgacaaat tgccggccgg attcggtcga gctacgcttc
     7081 tgaagtccct cgggagggca agcggcttcc agttaccctg ccttgcccac agccgccacc
     7141 ggcgcgccga ccggcgctgc ctcctgcggc ccgtccggct tgcgccccca cggcgccttg
     7201 gcctgttcgc gggtcttgcg gccgacatag gtgtcgaagc cgatctggac cgcgaccagc
     7261 atgtagacga agggctggaa ggcgatgccg acgaacaggc cgccgatcag gtagatgagc
     7321 tgggcgtgct ggagcgcggt ggcgagcggg gcgatccagg cctggtcgcc ctcggcgcgg
     7381 cggaagcggc ggcgcagcac ttccatgcgg acgaggccga tggcgtggat caggaggaac
     7441 aggatgaggc cggggaagcc ctgctcgccg agcatctcga aatagctcga atgataggcg
     7501 cgggcgctgt cctggatctg gctctggtcg acggtctgga ccgcgccggc ggtcttggtg
     7561 gcgaccgtgt tcacgcgcag cgaattctgc ttataggcct cgaagccgcc gcccatcggg
     7621 cggtccttca catattccca agtccagccc cacaccgcga tgcgggtcga ggcggaagcg
     7681 tcggcctcgt agctctggat ggtgctcatc cggctggtga aggtctgcgg caggaacggc
     7741 acggcgacgg cgagcgcgac cgcgaccaag gcgaggtagc cgatccggtt cctgacgtcg
     7801 cgcagcatca gcatgacgag ggcagcgatg cagacgaggc cggtgcgcgc ctcggtgccg
     7861 accgggatca ggaggcaggc gaagatgaga ttgtaggcga acacgcgcac gcgccagtcg
     7921 cggggataga tcgtgccgaa gcgggcgagc cagaggatga tcgggatgac cgcgatcgcc
     7981 accgtcgaga tggtgctgcc ctcgtaaagg ccgctattat tgtcgaccat caggttcaag
     8041 gcgccatagc cgccgcccga cgccgccgtc ttgatcccgc cgacgatgat gatcgccgcc
     8101 gccgacagag tcatgaacaa cagataggct tcgaggcgga gccgggtgcg gagcgtgaag
     8161 gggaggaaga tcgcccacat catcgccttc cacacccatt cccacttgaa cagggcgtcg
     8221 accgggaaat cggcatagat ggtggtgtag ccggcatagc cgagcaggat cagcatcagc
     8281 aattggcgcc ccgtcacgcg gaaatcgcgc ttgtcgtcgg cgaccagcca gccggcgatg
     8341 gcgaggccgg cgacgatcat cgacacaggc accgcgttca gcatgaagta gctcagccgc
     8401 tgcggcgcga cgatgtcgac ataggcgtag gcgagcacga acaggaacgg ccggcggaag
     8461 ccgaagccga tcagggccag caggaacccg acgaaggcga gatcacgcac cgggccgctc
     8521 cttcgcgccg atgcggcggt cgcggggcgc gggcggcgtc ggcgcaggga tggtgtcgtc
     8581 gtcgaggtcg gcgcgggtca gcagccgcca ggcggcgagg atcagcagcc catgggtgag
     8641 cccgagcgcg aaattgtcga tcatcctggc tggccccatg cgcgctgcat cgcctcgtca
     8701 taggagggtc cggttgacgc gccgttaagt ttgtcgtggc aaagcggctg tcgatgcgga
     8761 tccttcatgt gctcgaccac ggcctgccgc tgcacagcgg ctacacgttc cgcacgcggg
     8821 cgatcgtgaa ggcgcagctg gcgcgcggct gggaggtcgc ctgcctgacc gggccgcggc
     8881 acagcgcccc cgggccggac cccgagacga tcgacggcat caccttcttc cgcacgccgc
     8941 agccggcgcc ggcgccgtcg ccgctgcgcg aatggcgcga ggtgggggcg ctcgccgacc
     9001 ggctcgaccg gctggtgggg gagtggcggc cggaccagct tcatgcccat tcgccggtgc
     9061 tgaccgcgct cgcggcgctc cgggtggcga agcggcggcg gctgcctttg gtttacgaga
     9121 tccgggcttt ctgggaagat gccgcggtcg gcaacggcac cggccgcgaa ggctcggccc
     9181 gctaccggct gacccgggcg ctcgagacct gggcggcgcg gcgggcggat gcggtggcgg
     9241 tcatttgcga ggggctgcgg cgcgacctga tcgcgcgcgg catcgcgccc gagcggatca
     9301 tcgtcgctcc caacggcgtc gacatgagcc tgttcggcaa cccgcctgcg cccgacgcgg
     9361 cgctcgcggc gcggctgggg atcgagggcg acgtggtcgg cttcatcggc tccttctacg
     9421 attatgaggg gctggacgat ttgatcgcgg cgatgccgct gctgctcgaa cggcggccgg
     9481 ccgcgcggct gctgctggtt ggcggagggc cgatggagga aaggctgaag gcgcaggcgg
     9541 cggcctcccc cgccgcggcc cggatccact tcatcggccg ggtcccgcac gaagaggtcg
     9601 agctttatta cagcctgatc gacgtgctgg cttatccgcg caaggcgatg cggctgaccg
     9661 agctggtgac gccgctgaag ccgctggagg cgatggcgca gcgcaagctg gtcgcggcct
     9721 cggacgtggg cgggcatcgc gagctgatcg aggacggcgt caccggcacc ctgttccgcg
     9781 ccggcgaccc ggcggcgatc gccgaagcgc tcgaccggct gttcgccggg cgcgccggct
     9841 gggaccagcg ccgcgaggcc gcgcggagct tcgtcgagaa ggatcgtaac tggtcgtcaa
     9901 atatttcgcg ctatattccg gcatatcaac ggttaaccgg caaggttttg gcatgacatc
     9961 gaacacgatg aaacgggcga ggaagatcgc caagctgccg ctcgatctgc cgatggcggc
    10021 attggcggcg gcgtcggtgg cgttcgtcgc ttatgccatg cccgacgatc tgttctcgcg
    10081 gggggtcggc ctcaccgggc tgccgagcgt gttggcggcc gcggagccgc cgctcggcgc
    10141 gaccgcgcgg ctggcggtga tcgccgccgg cgcggccggc acctttctga tcgtgtggct
    10201 gttgctgcgc gcgctcggca agccgtcggc caaggccagg cgcctcgccg aggaggaggc
    10261 ggagatggcg ccgccccggc tgcgccgcgc cgacgcgcat cccgacgccc cctcgcgccg
    10321 gccgctgatc gcggggctcg atttgggcga gccgttcgac gcgctgccgc ggggccggca
    10381 atatcgcgag ccgcaggagg agcctgaagc gctcacccag tgggagccga gcctgccgag
    10441 cttcatgcag gtcgaaccgg acccgcggga agaggaggag gccgaggaac tcgagcttca
    10501 ggcagaggaa gcgttcgagg aagctgagcc gccggtggag gtgcccagcg
//
LOCUS       DASVRK010000185         7625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_149615, whole genome shotgun sequence.
ACCESSION   DASVRK010000185 DASVRK010000000
VERSION     DASVRK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149615"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4681)
                     /locus_tag="VF589_07285"
     CDS             complement(<1..4681)
                     /locus_tag="VF589_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8257419.1"
                     /translation="MSDGIAVIGMACRFPDADSPEALWQTVLARRRAFRRMPAERLDL
                     ADYDEARCDPSDSIYPIEAAVLENYSFDRARHLVPASAFAAADLTHWLALDVATEALE
                     DAGLAGGADHRARTGVVVGNTLTGEFSRAGLLRYRWPYVRRQLDSALAGEGFDPARRE
                     AFLAGFEERYKAPFPPPDEESLAGGLANTIAGRIANHHDLRGGGYTVDGACASSLLAM
                     ASACRRLQCGEVDLVLAGGVDLSLDPFELVGFARNGALSKSVMRVFDRRSDGFWPGEG
                     CGFVVLMRAAQASAEGRTARAVIRGWGVSSDGQGGLTRPTQAGQAQAMERAYAAAGFD
                     AGTVPLFEAHGTGTAVGDPVEIAAIAGVRGAAGAQFPAVIGSIKANIGHTKAAAGVAG
                     LIKQVMALDRQILPAATACDDPHPLLQEQAALVTTLRESRAWPAELPLRAGVSGLGFG
                     GINIHLALQAAGGPRRRSLDAKTRRQIRSTQDSELFLFAAENSAALRDSVEAFAALAP
                     SLSRAEMADAAAALARVCGAAPERAMVVAASPAELTRRIGLLLGRLKDAAGAAQSMGN
                     ALPSPRAAADETFLDLAEGIGFGCGSEPRILFLFPGQAAPAHLSGGAWRERFESVAKL
                     YPAESRAAADGGGTEVAQPAITLASRAGLAVLGTLGVKGWAALGHSLGELSALHWAGA
                     YDGAILQRLATRRGKAMAQRCLDGGTMLAIIAPPETAIELIEGSDAVIACYNGPRDCV
                     ISGSIEDLAAIEARARSRGIGVQPLAVSHAFHSRYAAPAAAAVAALLKREQIGALGRP
                     VYSSVAGRRLEPEDDLRALLGRQVAAPVRFGEALAAAMPKADLAIEIGPGHGLTRLAA
                     ELGGPPAVALDVGGESLAGLMLAVGSVFVLGGAASPAALFEDRFTRDFDLDRPRRFLA
                     NPCESAPSSDSRAGASPAIPGRPAAADPAVLDKAPIEVLRSLLARRLELAEEAIRPDH
                     RLFADLHLNSIAVGQIVGQAARALGISPPATPTNFALATVEEAAAALAKGTQIAGDAG
                     AAFPSGVDSWVRTFQPRFLLRPLSVGERGGPWRWRVVAAEGHPLARIFDENSCATGAA
                     AVAVCLPPSPDADDLTLILEAGQAALDAGADSLLLVVQSGGGGAAAARCLSLERPDLR
                     VVVVDLPFEDSRAAGWLLDEAAAARPGYTESRYDAGGARFCPVLETVSDPHTGPVQAV
                     LGPGDVLLVSGGGSGIGAECALAVARRSGARVAVIGRSPVESDAVRATMARFQAADVL
                     AAYEPADVSDAAAAACAVDRLTARLGPVTALIHAAGINRPVPLASLDLAALSDTVAVK
                     VAGFRNLIAALDPQRLKLAVGFSSIIARLGLPGEIHYAVANEWLGIAVQAFADTNPHC
                     RCLSLEWSVWSGVGMGERLGVIEGLARIGVAALPAPLATEMFESLIERAQSSTALVVA
                     GRFGSPLTAELLRGGDPGGRFLDRVALHYPGIEIVAECRLSLESDPYLEEHALAGTAL
                     FPAVLGLEAMAQAASALRGGAVPDSLEEVEFRRPVVAGAEGAVLRVAALARDSDRTEM
                     VLRAQESG"
     gene            complement(4678..5760)
                     /locus_tag="VF589_07290"
     CDS             complement(4678..5760)
                     /locus_tag="VF589_07290"
                     /inference="COORDINATES: protein motif:HMM:NF019624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1702 family protein"
                     /protein_id="HEX8257420.1"
                     /translation="MLNRGSINIDFDPKNNSAPAGASAAVRNLLRTRLEAARLGLFGI
                     SPHEAEFDVRGFRSTTTPRRDRLETVGRSFIAGYNAAVATGRGEAALEQLPGFPEDLR
                     GFVVEGAAMGLAMLDLVTPWPRRGFAAFVAGPAKPYVYLAYVGAGWALARTSWRLRGR
                     LGRVDPLLRWLMMDGYGFHSAYFQADRSIDLHRRPSLPAGYAARAFDQGLGRGLWFAM
                     GASGGAVRSAVESFVPERRADLWSGIGLAASYAGGAGTRELTDLAQVAGGFRLELAQG
                     AAFAAAAHLEGGAIPSHTDSACQVLCGMDARSAAAITSATRPNPASDGDGFHYEAWRA
                     AIRTAIFQHQANGARGRRRCAVELSA"
     gene            complement(5916..6923)
                     /locus_tag="VF589_07295"
     CDS             complement(5916..6923)
                     /locus_tag="VF589_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257421.1"
                     /translation="MKSATIAALRSKAAFFTAGVIALSGAACAASLQADAPRDPAGRA
                     TDPKGHAAESAQARANDDLSACANVRDPALSAPGHGSVAHLARLLHGTWVRRLTIAGV
                     AVETNSFLYFDMSGPEAGKGEALMIDLINQGWDGLASPAASLPGKRQAEPAQAAEARM
                     PATTGAYWVVSMKPAAPAAASRGRSGVALALAGDYRGTGAEYPPNGFRFTETGSFYGD
                     GPGYSTVQPWRAPPMALEAAPTTQPAQSAEAFTPIDLVAVRPGTVDPAGNRIGPRAPT
                     SEGQWEMALPRLTYVVCEGEIVDRYFKIDSATPTVGGKSLKAAWHSALETGLFSRGRF
                     N"
     gene            complement(6931..>7625)
                     /locus_tag="VF589_07300"
     CDS             complement(6931..>7625)
                     /locus_tag="VF589_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257422.1"
                     /translation="IDICYAVGYNSLGSFNNRFVSLVGCSPRRIRSLAARVDPAELRR
                     LIDGRAAIASPDAPDACSIWGTTSVTSGFSGVAVVALFLGPPSNAYPVASALCEGGAY
                     LLPPVPEDSDYFVMAVGLSWHEQMVDFLLQPNPLQAAALPPIHLSASGMASPIHFHLA
                     PRQPIDAPIPPSLALRLVEQFMRLPEELPKHRVRPSQRVLGLAAAPPRPSHRPAPYDS
                     RVAGPISLTPAE"
BASE COUNT         1288 a         2683 c         2569 g         1085 t
ORIGIN      
        1 agccactctc ttgggcgcgc aagaccattt ccgtccggtc ggaatcgcgg gccaaagcgg
       61 ccacgcgcag cacggcgccc tctgcgcccg ccaccaccgg gcggcggaac tcgacctctt
      121 ccaggctgtc gggaacggct ccgccgcgta gtgcggacgc ggcctgcgcc atggcctcca
      181 gccccagcac cgcggggaaa agcgcagtac ccgcaagggc atgctcctcc aaataggggt
      241 ccgattccaa actcaggcgg cattcggcga cgatctcgat cccgggatag tggagcgcga
      301 cccggtccag gaaacgtcct ccagggtcgc cgccgcgcag gagttcggcc gtcagcggag
      361 agccgaagcg cccggcaacc accaaggcgg tgctgctttg cgcacgctcg atcaggcttt
      421 cgaacatttc cgtcgccagc ggcgccggca aggcggcgac gccgatccgg gcgaggcctt
      481 cgatgacgcc gaggcgctcg cccatgccca ccccggacca gaccgaccat tccagcgaga
      541 ggcagcggca atgcggattg gtgtccgcga acgcttgcac cgcgattccg agccattcat
      601 tggccacggc ataatggatc tcgcccggga gccccagccg cgcgatgatc gagctgaacc
      661 caaccgccag tttcagccgc tgcggatcga gggctgcgat caggttgcga aagcctgcga
      721 ccttgactgc gacggtgtcg ctcagagccg ccaaatcgag gctggcgagc ggcaccgggc
      781 ggttgattcc ggcggcgtgg atcagcgcgg tgaccggtcc aagccgcgcg gtcaggcggt
      841 cgactgcgca agctgcggca gccgcgtcgc tgacgtcggc gggctcgtag gcggccagga
      901 cgtcggccgc ctggaagcgg gccatggtgg cccgaacggc atcggattcg accggggacc
      961 gcccgatcac cgctactctg gccccgctgc gccgagccac ggcgagcgcg cactccgcac
     1021 cgatcccgct cccgccgcca ctgaccagca gcacgtctcc cggccccagg acagcctgga
     1081 ccgggccggt gtggggatcg ctcacggtct ccagcacggg acagaagcgg gccccgccgg
     1141 catcatagcg gctctccgta tagccgggcc gagccgccgc ggcctcgtcc aaaagccatc
     1201 cggcagcgcg cgaatcctcg aacggcaggt cgaccacgac cactcgcaag tcgggccgct
     1261 cgagcgacag gcaccgggcg gccgcagcgc cgcccccgcc gctttggacc acaagcagca
     1321 acgagtcggc gccggcatcc agcgctgcct gtcctgcttc gaggatcaga gtcagatcgt
     1381 cggcatcggg cgacggcggc aggcacacag caacggccgc cgctccagtg gcgcaggagt
     1441 tctcgtcgaa gatgcgcgcc agcggatgcc cttcggccgc gacgacgcgc caccgccagg
     1501 gtccgccgcg ttcgcccacg ctcaggggcc gtaggagaaa ccggggttgg aacgtgcgga
     1561 cccagctatc gactccgctc gggaaggcag cgccggcgtc gccggcaatc tgggtgcctt
     1621 tggcgagagc cgcagcggct tcttcgactg tggccagcgc aaaattggtc ggggtggcgg
     1681 gcggcgagat accgagggca cgggcggcct gcccgactat ttggccgacc gcgatggagt
     1741 tgaggtgaag atctgcgaac agccgatgat ccggcctgat cgcctcttcc gcaagttcca
     1801 ggcggcgcgc gagcaagctt cgcagcacct cgatcggcgc cttgtcgagc acggccgggt
     1861 ctgctgcggc cggtcggccc gggatggcgg gcgacgcacc ggcgcgggag tccgaggaag
     1921 gggcgctctc acaaggattg gcgaggaagc ggcgcggccg gtcaagatcg aagtcgcgcg
     1981 tgaaccggtc ctcgaacagg gccgccggcg aggccgcgcc gccgaggacg aacaccgacc
     2041 ccaccgcaag catcagccca gccaaggatt cgcctccaac atcgagcgcc actgccggcg
     2101 gcccgccaag ctcggccgcg agccgcgtca gcccatgccc cggcccgatt tctatcgcca
     2161 gatcggcctt gggcatggcc gccgccaaag cctccccgaa gcgcaccggc gccgcgacct
     2221 ggcggccgag cagtgcgcgc agatcgtcct cgggctcgag ccggcgccct gcgacggatg
     2281 agtagacggg ccgccccaaa gcgccgatct gctcccgttt caaaagcgcg gccacagcgg
     2341 cggcggccgg cgccgcgtag cgcgagtgaa aggcatgtga cactgcaagc ggctggacgc
     2401 cgatgccgcg ggaacgggcg cgcgcctcga tcgcagcgag atcctcgatc gagccggaga
     2461 ttacgcagtc gcgtggaccg ttatagcagg cgatcacggc atcgctgccc tcgatcagtt
     2521 cgatggctgt ctcgggcggc gcaatgatcg caagcatggt tccgccgtcg aggcagcgct
     2581 gcgccatcgc tttgccgcgc cgggtggcga ggcgctgcag gatggcgccg tcataggcgc
     2641 ccgcccagtg cagcgcgctc aactcgccca ggctgtggcc gagcgccgcc caacccttca
     2701 cccccagagt ccccagtacg gcaaggccgg cgcgtgatgc cagggtgatc gccggctggg
     2761 cgacctcggt gcccccgcca tccgcggcgg cgcggctttc ggcgggataa agcttcgcaa
     2821 cgctctcgaa ccgctcccgc caggctcccc cggagagatg ggcgggagcc gcctggccag
     2881 gaaacagaaa cagaatccgt ggctcggagc cgcagccgaa acctattcct tccgccaggt
     2941 cgaggaacgt ctcatccgca gccgcccgcg gggacggtaa cgcgttaccc atgctttgtg
     3001 ccgcgccagc cgcatctttc agccggccga gcagcagccc gatccgccgg gtcaactcgg
     3061 cggggctggc ggcgaccacc atggcgcgct ccggcgccgc accgcaaacc ctggccaaag
     3121 cggcagcggc atccgccatt tcggcgcgcg acaatgaagg cgcgagcgcg gcgaaggctt
     3181 cgacgctgtc acgcagcgcc gcgctattct cggcggcgaa caggaacagt tccgaatcct
     3241 gcgtgctgcg gatctggcgc cgcgttttgg catcgagcga ccgtcggcgc gggccgccgg
     3301 ccgcttggag ggccagatgg atgttgatcc cgccaaagcc caggccgctg acccccgccc
     3361 gaagcggcag ctcggccggc caggcgcggc tctccctgag cgtggtgacg agcgcggcct
     3421 gctcctgaag cagcggatgg gggtcgtcgc aggccgtggc agccggaagg atctggcggt
     3481 ccagggccat gacctgcttg atcagcccgg cgactccggc ggccgccttg gtatggccta
     3541 tgttcgcctt aatggagccg atcaccgccg gaaattgcgc gccggccgcc ccgcgcaccc
     3601 cggcgatggc cgcgatctcc accgggtctc cgaccgccgt gccggttccg tgagcctcga
     3661 acagcggaac ggtcccggca tcgaatcccg cggccgcata ggcgcgctcc atggcctggg
     3721 cctggccggc ctgggtcggt cgcgtcagcc ctccttggcc atccgaggag acgccccagc
     3781 cgcggatcac ggcgcgcgcc gtgcggcctt cggcgctggc ctgcgccgcc cgcatgagga
     3841 cgacgaaacc gcagccctcg cccggccaga agccgtccga gcgccggtcg aacactcgca
     3901 tcacgctttt cgacagcgcc ccgttcctgg cgaaaccgac aagttcgaaa ggatccaggc
     3961 tgagatcgac gccgccggcc aggaccaggt ccacctcccc gcattgcaac ctgcggcacg
     4021 ccgacgccat cgcgagcaaa gacgaggcgc aggcgccgtc gaccgtatat cccccgcccc
     4081 gcagatcgtg gtgattggcg atccggccgg cgatcgtgtt ggccagtccg ccggcaaggc
     4141 tttcttcgtc gggcggcggg aacggcgcct tgtagcgctc ttcgaagccc gccagaaacg
     4201 cttcccggcg cgcggggtcg aacccttccc cggcgagcgc ggagtcgagc tggcgccgga
     4261 catagggcca gcgatagcgc agcagcccgg cgcggctgaa ttcgccggtg agcgtattgc
     4321 ccacgacgac gccggtgcgg gcccgatggt ccgccccgcc cgccagcccg gcatcctcca
     4381 gcgcctcggt cgcgacgtcg agggcgagcc agtgagtgag gtccgcagcg gcgaaggccg
     4441 aggccggcac caggtggcgc gcccgatcga agctgtaatt ctcaaggacg gccgcctcga
     4501 tcggatagat cgaatccgac ggatcacacc gagcctcgtc gtaatcggcc agatcgagcc
     4561 gctcggcggg cattcggcgg aaggcgcgcc gccgggccaa caccgtctgc cacagcgcct
     4621 cgggtgaatc cgcatcgggg aagcggcaag ccattccgat cacggcgatt ccgtcgctca
     4681 tgcgctcaac tctaccgcac agcgccggcg gcctcgcgcc ccgttggcct gatgttggaa
     4741 gatcgcggtc cttatcgccg cccgccaggc ctcataatgg aagccgtcgc cgtccgaagc
     4801 ggggttcggc cgtgtcgccg acgtgatcgc tgccgccgac cgcgcgtcca tgccgcacag
     4861 cacctggcag gcggagtcgg tgtggctcgg aatcgcgcct ccttcgagat gcgcggcggc
     4921 ggcaaaagcg gcgccctgag cgagttccag gcggaatccg cccgccacct gcgcaaggtc
     4981 ggtcagctcc cgggttccag ctccgcctgc ataggatgcg gcgaggccga ttccgctcca
     5041 gagatccgcc cgacgctccg gaacgaagga ttcgaccgcc gagcgcaccg ctccgccgga
     5101 tgctcccatg gcgaaccaga ggccgcggcc gaggccttgg tcgaatgcac gggcggcata
     5161 tccggcgggc aggctcggcc ggcggtgcag gtcgatcgaa cggtccgctt gaaaataggc
     5221 ggaatggaag ccgtagccgt ccatcatcag ccatcgcaac agggggtcga ctcggccgag
     5281 gcggccgcgc aagcgccagg acgtgcgggc cagcgcccag cccgctccca cataagccag
     5341 gtagacatag ggcttggcgg ggcccgcgac aaaagcagcg aaaccgcggc ggggccaagg
     5401 cgtcaccaga tcgagcattg ccaggcccat cgccgctcct tctacgacga agcctcgcag
     5461 gtcctcggga aacccgggaa gctgctcgag ggcggcttcg ccgcgcccgg tggcgacggc
     5521 cgcgttatag cctgcgatga agctccgccc aacggtctcc agccgatccc gccgcggcgt
     5581 agtggtcgac cggaagcccc gcacgtcgaa ctcggcctcg tgcggagata ttccaaaaag
     5641 tccgaggcgg gcggcctcaa ggcgggtgcg gaggagatta cgaacagcgg cggacgcgcc
     5701 ggctggtgcg ctattatttt tcggatcgaa atctatgttg atagatccac gattgagcat
     5761 tgcccctgcc gcctgctcag agttgcgggc ataacggcat actattcagc gattttcaat
     5821 tgctgttttt gcacgtcgtt tccgaaattt aacctgtcaa aacccgtcga aagcattccc
     5881 ctgccgcctt ttctcttaag ctgcatcacg gcgattcaat tgaagcgccc gcgcgagaac
     5941 aggcccgtct cgagagcaga gtgccaagct gccttcagcg acttgccgcc gactgtcgga
     6001 gtggcgctgt ctatcttgaa ataccggtcg acgatctctc cttcgcagac gacgtaggtg
     6061 agtctcggca gagccatctc ccactgtcct tcggacgtcg gcgctctcgg cccgatcctg
     6121 tttcccgccg gatcgaccgt gcccggccgt acagccacca aatcgattgg ggtgaaggct
     6181 tcggcagact gcgccggctg cgtcgtcggc gccgcctcca gagccatcgg cggcgcccgc
     6241 cacggctgca cggtcgaata gcccggaccg tccccataga aactgccggt ttcggtgaaa
     6301 cggaagccgt ttgggggata ttcggcgccc gtgccgcgat aatcgcccgc cagcgccaga
     6361 gccactcctg aacggcccct gctggccgca gccggcgcgg ccggcttcat ggacaccacc
     6421 cagtaggcgc cggtggtcgc tggcatccgc gcttcggccg cttgcgcggg ctccgcttgc
     6481 cttttgcccg gcagcgaagc cgccggcgac gccagaccgt cccagccctg attgatcagg
     6541 tcgatcatca aggcctcgcc cttgccggct tcggggccag acatgtcgaa ataaaggaac
     6601 gagttcgtct ccaccgccac cccggcgatc gtcagccggc gcacccaggt gccatggagc
     6661 aaccgagcca ggtgcgcgac gctgccgtgc cccggcgcgg acagcgcggg atcccgaaca
     6721 ttggcacagg cgctcaggtc gtcgttggcc cgagcctgtg ccgattccgc ggcatggccc
     6781 ttcgggtcgg tcgccctgcc cgcgggatcc cgcggcgcgt ccgcctgcag ggaggcagcg
     6841 catgccgccc ccgacagcgc gattacgccg gcggtaaaaa aagccgcctt gctgcgcagt
     6901 gcagcgatgg tcgctgattt catagacgca tcactcggct ggagtcaggc tgatcgggcc
     6961 cgccactcga gagtcgtacg gggccggacg atggctgggg cgcggcggcg ccgcggcaag
     7021 gccgagcact cgctgcgagg gccggaccct gtgcttgggc aactcctccg gcaggcgcat
     7081 gaactgctcg acgagccgga gcgccaggga cggcgggatt ggcgcgtcga tcggctgcct
     7141 tggcgccagg tggaagtgga tcgggctcgc catgccgctg gccgagaggt ggatcggcgg
     7201 caatgcggcc gcctgcaaag gatttggttg gagcaggaaa tcgaccattt gctcgtgcca
     7261 gctgagccca acggccatga cgaagtaatc gctgtcctcc gggaccggcg gaagcagata
     7321 ggcaccccct tcacacagag cgctcgccac cggataggca ttggagggag gtcccaggaa
     7381 aagagccacc acggcgactc cggaaaagcc gctggtcaca cttgtcgttc cccagatgga
     7441 gcaggcatca ggagcgtcgg gcgacgcaat cgccgctcgg ccatcgatca gtcggcgcaa
     7501 ctcggccgga tcgactctgg ccgccagact gcgaatgcgc cgaggcgagc agccgaccaa
     7561 cgacacgaac cgattgttga agctgccgag gctgttgtac ccgacggcat agcagatatc
     7621 gatga
//
LOCUS       DASVRK010000186         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_472321, whole genome shotgun sequence.
ACCESSION   DASVRK010000186 DASVRK010000000
VERSION     DASVRK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472321"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2274)
                     /locus_tag="VF589_07305"
     CDS             complement(<1..>2274)
                     /locus_tag="VF589_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257423.1"
                     /translation="TGRDTLSGGAGFDFFYLADGDAEAGESINGGADTDRIETASARL
                     DLSGVTINELEAIQTVRGAGTAFIVDTAAHALLVNGGGGYDSLTVNDEALTAQQRNAI
                     FAQGIETITDAAGTHGPNVAPSITSSGGGSFANLSLQENGNLIAQLTAADPDAGETLT
                     WSISGGQDSAKFQINADNGVLSFLAAPDFENPTASGGGNVYGVTVRVSDGNGGFDEQN
                     VFVEITDLNNFVGNGADNRIAGDNGADTMTGYGGNDTYIVDHAGDRVVEGVGGGIDVV
                     QASVTFDLRGQEIENLTLTGFAQINGIGNHRANRLNGNSADNLLNGFGAADIMRGFNG
                     SDIYYVDDADDQVIEGLTGDADRVVTSVSYTLAALSEVERLQTSNAALTTAISLGGNE
                     YANAITGNAGVNTINGGGEADVMTGLDGSDNYYVNHAGDRVVEGLTGDADRVFASVSY
                     RLATGSEVERLQTTDAALTTAIDLFGNEYANALTGNAGENLLNGGGAADTMTGLDGSD
                     DYIVNDGSDRVVEAATGDVDRIFTSVSFRLGVGSEVERLQAANAALTTAIDLGGNEFA
                     NTITGNAGDNFINGGGAADRMTGLDGSDTYFVDDAADVVLEGLTGDIDRVFARVSYAL
                     AEDSEVERLQTTDAALTTAINLTGNEFANAITGNAGLNILDGGGGNDILTGLDGSDTY
                     LVDRAGDIVMEGLTGDFDKIRTSTSYRLAVGSEIEALLTDRSTATTAINLTGNEYGNS
                     IIGNSGINRLSGGGGNDV"
BASE COUNT          345 a          736 c          764 g          429 t
ORIGIN      
        1 acatcgttgc cgccgccgcc cgacagccgg ttgatgccgg aattgccgat gatgctgttg
       61 ccatattcgt tgccggtgag gttgatcgcg gtggtggcgg tgctgcggtc ggtcaacagc
      121 gcctcgatct ccgaacccac cgccagccgg tacgaggtgc tggtgcggat cttgtcgaag
      181 tcgccggtca gcccttccat gacaatatcg ccggcgcggt cgaccagata ggtgtcggac
      241 ccgtcgaggc cggtgaggat gtcattgccg ccgccgccgt cgaggatatt gaggccggca
      301 ttgccggtga tcgcgttggc aaattcattg cccgtgaggt taatcgcggt ggtcagcgcg
      361 gcgtccgtgg tctgcagccg ctcgacctcc gaatcctccg ccagagcgta gctcacccgg
      421 gcgaacaccc ggtcgatgtc gccggtcagg ccctcgagga cgacgtcggc ggcatcatcg
      481 acgaaataag tatccgatcc gtcgagcccg gtcatccggt cggcggcgcc gccgccgttg
      541 atgaagttgt cgcccgcatt gccggtgatg gtgttggcga actcgttgcc gccgaggtcg
      601 atggcggtgg tgagcgcggc attggccgcc tgcaaccgct ccacttcgga gccgaccccg
      661 agccggaagc tgaccgaggt gaagatccgg tcgacgtcgc cggtcgcggc ctcgacgacc
      721 cgatccgaac cgtcgttgac gatataatcg tccgacccgt cgagccccgt catggtgtcg
      781 gccgcgccgc cgccgttgag cagattctcg ccggcattgc cggtgagggc gttggcatat
      841 tcgttgccga acaggtcgat cgcggtggtg agcgcggcat cggtcgtctg cagccgctcg
      901 acctcggaac ccgtcgccag ccggtagctg accgacgcga acacgcggtc cgcatcgccg
      961 gtcagcccct cgaccacccg gtccccggca tggttgacat aataattgtc ggagccgtcg
     1021 aggcccgtca tcacatcggc ctcgccgccg ccgttgatgg tattgacgcc ggcattgccg
     1081 gtgatggcgt tggcatattc gttgccgcca agactgatcg ccgtcgtcag cgcggcattg
     1141 ctggtctgca gccgctcgac ctcggataag gccgccagcg tgtagcttac cgaagtgacc
     1201 acgcggtcgg catcgccggt cagcccctcg atgacctgat cgtcggcatc gtcgacataa
     1261 tagatatccg agccgttgaa gccgcgcatg atgtcggcgg cgccgaagcc gttcagcaga
     1321 ttgtcggcgc tattgccatt gagccggttg gcccggtgat tgccgatgcc gttgatctgg
     1381 gcgaagccgg tgagcgtgag attctcgatc tcctggccgc gcaggtcgaa ggtgaccgag
     1441 gcctgcacca cgtcgatacc gccgcccacg ccctcgacca cccggtcgcc ggcgtggtcc
     1501 acgatgtagg tgtcgttgcc gccgtagccg gtcatggtgt cggcaccatt gtcgccggcg
     1561 atgcggttgt cggcgccatt gccgacgaaa ttgtttaggt cggtaatctc gacgaacaca
     1621 ttctgttcgt caaacccgcc attgccgtcc gagacgcgca cggtgacgcc gtagacgttg
     1681 ccgccgccgg aagcggtcgg gttctcgaaa tcgggcgcgg ccaagaagct gagcacgcca
     1741 ttgtcggcgt tgatctggaa cttcgcggaa tcctggccgc cggagatcga ccaggtcagc
     1801 gtctcccccg catcggggtc ggccgcggtc agctgggcga tcagattgcc attctcctgg
     1861 aggctgagat tggcgaagga gccgccgccg ctcgacgtga tcgacggcgc gacattcggc
     1921 ccgtgcgtgc cggcggcgtc ggtgatcgtc tcgatgccct gtgcgaagat cgcgttgcgc
     1981 tgctgcgcgg tgagggcctc atcgttgacg gtcaggctgt catagccgcc cccgccattg
     2041 accagcaaag catgggcggc ggtgtcgacg atgaacgccg tgcccgcgcc gcgcaccgtc
     2101 tggatcgcct cgagctcgtt gatggtgacg ccgctgagat cgagccgcgc gctcgcggtt
     2161 tcgatgcggt ccgtgtcggc gccgccgttg atcgactcgc cggcctccgc gtcgccgtcg
     2221 gcgaggtaga agaagtcgaa gcccgcgccg ccgctcagcg tgtcgcggcc ggtg
//
LOCUS       DASVRK010000187        14465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_149932, whole genome shotgun sequence.
ACCESSION   DASVRK010000187 DASVRK010000000
VERSION     DASVRK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149932"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VF589_07310"
     CDS             complement(<1..689)
                     /locus_tag="VF589_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257424.1"
                     /translation="MAETVRFNITTDEPVQQNTGSGLDRVIIEATPPTLPNQVRITFT
                     SSEVGNGNPNDSGTMSGQDGGLAVRVQAEDGQGFVTGQISRFDDEGIRFESASPGITF
                     DVRDLIDGVERGDQFEVVLLGTSQDDRFDYADEDRNLYINGGMGNDTIRGGTGDDYLE
                     GGFGDDRLFGGFGDDRLHGGLGRDYIAGGRGDDSLVYNLSTDDMDRVDLGEGDDTVNI
                     AADENVAQIRVT"
     gene            complement(958..1572)
                     /locus_tag="VF589_07315"
     CDS             complement(958..1572)
                     /locus_tag="VF589_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257425.1"
                     /translation="MRLMIGLLGCATLAACGPSQSEGVTAELAEESAGLTIALPEAEL
                     PAPEAAPEAPGNSAAAEPAVEPEAAPPAKAAELPAPPPPPPPAEKAADKAPPEKEAVA
                     EAAAPAPAPAGADEVEAVASGEAARMRLLPARPETSVAPWAGHIRRAGFACGSVASAR
                     QLQRPDGRTLEIYKIDCSSGGSYQGTIKRGRLYFRTWTGRLDRR"
     gene            complement(1683..2147)
                     /locus_tag="VF589_07320"
     CDS             complement(1683..2147)
                     /locus_tag="VF589_07320"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8257426.1"
                     /translation="MLEEGDPVPDVKLLGMEGQSVSPADFRGNKLVLYFYPKDDTSGC
                     TREAQDFTALAGEFERTGTWILGVSKDEAKKHRKFIDKYDLKIPLATDPDGSVCEAFG
                     TWVQKSLYGRKYMGIDRATFLIDSDGVIKRVWRKVSVPGHAEAVLAAARELP"
     gene            complement(2149..4851)
                     /locus_tag="VF589_07325"
     CDS             complement(2149..4851)
                     /locus_tag="VF589_07325"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX8257427.1"
                     /translation="MVETEGYRDIDDALRRARAHSPFLKLQLDAFPAVAEALAGGSVD
                     AALAAARTAGDGAPDLAAALRRERSATALALGIGDLAGALTLEQVVEALSDLADLALE
                     QALSAIIEARTPGASPTGFAVIALGKHGSRELNYSSDIDVLFLFDPTTLPLKPREDAG
                     QAAVRIGQRLVDLLQKRDGEGYVFRVDLRLRPSSEVTPIALPVDAAISYYESSALPWE
                     RAAFIRARMVAGDPALGRYFMEAIHPFVWRRSLDFGAIGELHSITRRIRDHYAQGQRF
                     GPGFDLKRGRGGIREIEFFAQMHQLIHGGREPGLRPAATLNALSALAAAGRIPVPQAE
                     TLARVYRLLRTMEHRLQMVDDRQTHSLPAGAAALDNVAQLHGLDGAPALLDLLAEPVA
                     EVAAIYDELGEGGGDRLPHDSAALEARLARAGFDDPVAGRLRIESWRSGRARSLRTAP
                     ARGAFEAMLPVLIDAFGASPDSMRAMNRFDDVVGRLPSGVNFYRLLEARPGLAQLLAT
                     VLSHAPALAEQLGRRPQLLDGLIDASAFQPAPPAAQLIRDFARSERAGEDYQLVLDRV
                     RRRVNERRFALGVQLVVARRDPLEVAEGYSRVAEAAINVLADAAVAEFERAHGRVPGS
                     ELVILGLGRLGGEALTHASDLDLIYLFSGTHEAESDGRKPLRASDYFNRLAPRVTAAL
                     SVATAAGPLYEVDTRLRPSGKDGLLAISLPSFAEYQRERAWTWEHMALTRARPVYGSP
                     EARASLARIVEATLAMPRDPAKVTADAARMRADIAAHKPPGGPFDIKLGDGGLVDLEF
                     AVHTLQLRHRVGLSPRLEDALAALAAEGLVSDGIDPALRLLTRMLVTFRLVSPSSAEP
                     PPASRGLVAKACGAADWAALLAAHDEARQRVFELWRAVASFEGE"
     gene            4957..5106
                     /locus_tag="VF589_07330"
     CDS             4957..5106
                     /locus_tag="VF589_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257428.1"
                     /translation="MVNASSRRFVKVAPPTVHTGVGNALREAFAMDGQVRSLQMFEDL
                     LAKLD"
     gene            complement(5109..5771)
                     /locus_tag="VF589_07335"
     CDS             complement(5109..5771)
                     /locus_tag="VF589_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8257429.1"
                     /translation="MAGEKPALIDEDDVAEVPREEPVPLPDNEFKDQLGQVIPHLRAF
                     GRSLSGSRDLADDLVQETLLKAWAARKRFQAGTNMRAWTFIILRNLFLSQMRRARFKG
                     EWDELTASKLLAAPASQDRHVELGDLQRALMHLPQPQREALILVGAGGFAYEEAAEIC
                     GCAVGTIKSRVARGRVALETLLAEGKLPSRRDHETDANKTALQSIMSEVDDLSRDRDP
                     QD"
     gene            complement(5862..5999)
                     /locus_tag="VF589_07340"
     CDS             complement(5862..5999)
                     /locus_tag="VF589_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257430.1"
                     /translation="MDDGSPHIGKTEARGGTTPHMTRYVLSIGLVLVIILFALILFVW
                     R"
     gene            complement(6075..6872)
                     /locus_tag="VF589_07345"
     CDS             complement(6075..6872)
                     /locus_tag="VF589_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8257431.1"
                     /translation="MSLGQELAPHLPFVRRYARALTGTQAHGDAFVRATLEAIVAAPD
                     DFPRDVDARLGLYRTFHAIWSSANIEDDPSSLDASEAEGIAQARLARITPLSRQALLL
                     TAMEGFTPEDTGYLIGASAEEVDNLVAEALAEIERQTRAEVLIIEDEPIIAMDIETIV
                     RDLGHTVTGVAVTRDEAVSQAMARRPGLVLADIQLADDSSGIDAVKDILQEFSVPVIF
                     ITAFPERLLTGERPEPTFLITKPFQRSTVKAAIAQALFFDSATVPAA"
     gene            7173..7379
                     /locus_tag="VF589_07350"
     CDS             7173..7379
                     /locus_tag="VF589_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257432.1"
                     /translation="MTFAHDKDAGSGRKPDRAAASDAGERTPPKGRKRPVTPELGHAL
                     RNAYQRTIDEDIPPEMLDLLGKLG"
     gene            7408..8994
                     /locus_tag="VF589_07355"
     CDS             7408..8994
                     /locus_tag="VF589_07355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8257433.1"
                     /translation="MSQEQPSHEEQGWFASRFARLSTGLKMFLILSLALFPLGLIAIL
                     ASLESARDNSAGRAQHIFGRLEVKAQRINSLLSRSSGTIRAASAAIAVSPGSDVCRVT
                     LARLARAQVTTGRYALYGGGPRPRCATPGFAPAPIANPERRPSIIEISPDGRILRFAL
                     YDDADNPEGMGEFTRESLAELTLLPGTRGNFDLELLQGGRGMMLRNGYQGGPLVQSVT
                     GTEPVAGDRLQLRIRQSAVPISPVELLMILLPVMMWLGAGIIGWLILDRLLLKPLVRM
                     QRAVAAYRPGDQHLDLPAISSPAREIGELGAAFDRVTQTVARHEAELEAAVERQRRLV
                     REVHHRVKNNLQVVASLLNLHARGVANEEVAAAYASIQRRVDALAVVHRNHYAELEEN
                     RGVALRSLISELGANLRATAPASAGAMAIRIDIEPYYATQDVAVSVAFLITELVEYAM
                     FCGARAVSISLTGGRGPAALLAVESDSLIAGAECAPELTERFERIVTGLSRQLRSTLD
                     RDAERGRYALEIAVVDKAER"
     gene            complement(9127..9315)
                     /locus_tag="VF589_07360"
     CDS             complement(9127..9315)
                     /locus_tag="VF589_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257434.1"
                     /translation="MAKPPQSTPHSDIDGVHQDERRNVDTAHDLGQSAGTVEDAKRRS
                     VGRPPRADDLDRPEDSSG"
     gene            9763..9888
                     /locus_tag="VF589_07365"
     CDS             9763..9888
                     /locus_tag="VF589_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX8257435.1"
                     /translation="MRKILAIFALSTSLALAACNTVEGVGEDVQSAGEAVEDAAD"
     gene            complement(9966..11810)
                     /locus_tag="VF589_07370"
     CDS             complement(9966..11810)
                     /locus_tag="VF589_07370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX8257436.1"
                     /translation="MRDQFEPWGRTARIEGEAFARAEANEATVSDYTALISGSSLHGR
                     SGIGAFCSFSFPTSVPDYYEGVYPEEGLASFRPMTQAEKAVAREAIAAWGEACGLTFF
                     EVAPGDGDIKFMSFDLALVTPDQFDAAFAYLPGGQYGDGGFQSDVFLDFRWADDLHIQ
                     LHEIGHAIGLKHPFEGSPTLDPDRDHYGETVMSYSSDGFPGDVLGPLDRDAVQFLYGD
                     ADADGGQAASWSWDPAARRLSQTGSETADILLGIGGRDSIDGRGGDDFLNGREGADRL
                     AGGAGRDELSGGWGDDVLDGGDHADQLYGGWDADALSGGLGADLLNGGVGDDLLAGGE
                     GADTYQGHDGFDTVSYQGEARGVTVALNQGRYIEALQRWENMSEVEALIGSSFADALT
                     GDGGANRLDGGSGADRLEGGGGDDRLDGGAGLDTASFRSAAAGVRASLAVAGAQDTLA
                     GLDTLLGVERLEGSAFADALTGNAGANLLSGLDGADRLAGGAGADRLAGGAGRDFALG
                     GAGADQFLFANGDFGGAVARSADRIAGFEAAADRINLRAVDAVAGGADDRFTFVGEAA
                     FSGTAGELRWHHANGATLVSGDVDGDGKADFMIRLDGAIALTAAEFVV"
     gene            complement(11864..13552)
                     /locus_tag="VF589_07375"
     CDS             complement(11864..13552)
                     /locus_tag="VF589_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX8257437.1"
                     /translation="MEDGPGWTHPPPPREGCRVTPLIEAADMYPMLEHLVLDARQSVW
                     LSFRVFDPNTKTRSERAVAAGIEDWAQLIRDAVRRGVEVRILLADFEPVLADHLHAGS
                     WNTYRRIRELVEELGDSHPERLQLMIVQHEGELGWGWRQLLRLLLRRRIRKVVAELLA
                     KDEHDDGGFDTRPGLWRHVDWHEGKPKSWKPAPPPRLWPATYHQKFVVVDRQKAIIGG
                     LDLDERRWDDRRHRQRADQTWHDISALVEGPVVADAARHFARLWNREMPRYRDTVGEW
                     TGECGRRLMLDPLSEVPIEGIAEPERAGEAKVQLVRTLSRKSASFFATGPVHGIRELK
                     AAHRELILSAKRQLYVEAQFFRSEAAADWIEEALSSTAELEVIILVANTPEEIAFEGQ
                     TDNLAHRHGEYLQARALGRLLEKAGPQRVGLFTLAKHEAVKRSEEGFEETRGTAFGSG
                     LIHIHSKLLIADDATCLLSSANINGRSFEWDTELGFLWTEEGEAIFEFKRRLWWQLFG
                     GALPDSMDLEGWRDAARANSAAEPHERRGFVLPYQLGRARRFARPYWFIPDDLV"
     gene            complement(13630..14403)
                     /gene="tatC"
                     /locus_tag="VF589_07380"
     CDS             complement(13630..14403)
                     /gene="tatC"
                     /locus_tag="VF589_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX8257438.1"
                     /translation="MSEPDDTKAPLMEHLIELRRRLMWSFAALFAAFGVCFYFADRIF
                     AFLAQPLVKAFPPGEGKLVYTKLYEAFFVEVRVAVFAAFFLAFPVIANQIWLFVAPGL
                     YSKEKRAFLPFLIATPVLFTLGGALAYYGVMPYAFHFFLSFQGNLGGIEQEALPAVGE
                     YLSLVMHFILAFGIAFLLPVLLMLLERAGLVTRDQLKRGRRYAILVAFIIAAVATPPD
                     VLSQFMLAVPLIVLYELSLIAIWFTERRRARETTGSELL"
BASE COUNT         2279 a         5013 c         4915 g         2258 t
ORIGIN      
        1 gtcacgcgga tctgcgcgac attctcgtcg gccgcgatgt tgaccgtgtc gtcgccctcg
       61 ccgaggtcga cgcggtccat atcgtcggtg gagaggttgt agaccagcga atcgtcgccg
      121 cggccgccgg cgatataatc ccggccgagc ccgccgtgga gccggtcgtc gccaaatcca
      181 ccgaacagcc ggtcgtcgcc gaacccgcct tcgagatagt cgtcgccggt gccgccgcgg
      241 atggtgtcgt tgcccatgcc gccgttgatg tagaggttgc gatcctcgtc ggcatagtcg
      301 aaccggtcgt cctggctggt gccgagcagc acgacctcga actggtcgcc gcgctcgacg
      361 ccgtcgatca ggtcgcggac gtcgaaggtt atgcccgggc tggcgctctc gaagcggatg
      421 ccttcatcgt cgaaccggct gatctggccg gtgacgaagc cctggccgtc ctcggcctgc
      481 acccgcacgg cgaggccacc atcctggccg ctcatcgtgc cgctgtcgtt cggattgcca
      541 ttgcccactt cggagctggt gaaggtaatc cggacctggt tgggcaaggt cggcggagtg
      601 gcctcgatga tcacccggtc gagcccggag ccggtattct gctgcaccgg ctcgtcggtg
      661 gtgatgttga acctgacggt ttcagccatg gcggcttgag tttgagcgtc cctgcggttg
      721 tcggccataa tctcgatact ccctgaagcg cgcgcgcgct gtcgtcacaa gcccggcggt
      781 gccgggcgaa gggtcctttg cggacggccg gtcggccttt cccttcggcg cgagatacga
      841 agcaggcggc gaatggatgc attcgagcgg caggcggttc gccggaaggg acgatgcgaa
      901 agacgggcgc caagccgttg tgctggggcc gaaacgatcg ctgcgtgttt ggcgccctta
      961 ccgccggtcg aggcggccgg tccaggtgcg gaaatagagc cgtccgcgct tgatggtgcc
     1021 ttggtaggag ccgcccgagg agcaatcgat cttgtagatt tcgagcgtcc gcccgtccgg
     1081 ccgctgcagc tggcgcgccg aagcaacgct gccgcaggcg aagccggcgc gtcggatgtg
     1141 gccggcccag ggcgcgacgc tggtttcagg ccgggccggc agcagccgca ttcgcgccgc
     1201 ctcgcccgat gcgaccgcct cgacttcatc cgcgcccgcg ggcgccggcg ccggcgccgc
     1261 ggcctcggcc acggcttcct tctccggcgg agccttgtcg gcggccttct ccgcgggcgg
     1321 cggcggcggc ggcggggcgg gcagctccgc agccttggcc gggggcgcgg cctcaggctc
     1381 taccgcgggc tcggcagcgg cgctgttgcc cggtgcttcg ggcgcggctt cgggagcggg
     1441 cagctcagct tccggcaagg caatggtgag gccggcgctc tcctcggcca gttcggccgt
     1501 cacgccctcg gactgggagg ggccgcaggc cgccagcgta gcgcatccca ataagccgat
     1561 cataagccgc accgcgattc tcccgccttg ccaagggcga ggctagctca gtcgggcggc
     1621 ctttgcccat cccttccggc gccatgacgt cattcttgga gcgcgggtcc ggcgcccggg
     1681 gatcagggaa gctcccgcgc cgccgccagc accgcctcgg cgtggccggg aacgctgacc
     1741 ttgcgccaca cccgcttgat gacgccgtcg ctgtcgatga ggaaggtggc gcggtcgatg
     1801 cccatatatt tgcggccgta gaggctcttc tggacccagg tgccgaacgc ctcgcagacc
     1861 gagccgtccg ggtccgtcgc cagcgggatc ttgaggtcat atttgtcgat gaacttgcgg
     1921 tgcttcttcg cttcgtcctt ggagacgccg aggatccagg tgccggtacg ctcgaattcg
     1981 ccggccagcg cggtgaaatc ctgcgcctcg cgggtgcagc cggacgtgtc gtccttcggg
     2041 tagaaataaa gcaccagctt gttgccgcgg aaatcggccg ggctgaccga ttgcccctcc
     2101 attcccagga gcttcacgtc gggtaccgga tcgccttcct cgagcatgtc actctccctc
     2161 gaacgacgcg accgcgcgcc acagctcgaa caccctctgc ctcgcctcgt catgcgcggc
     2221 aagcaaagcc gcccagtcgg cggcgccgca ggccttggcg accagaccgc gcgaggccgg
     2281 cggcggctcg gccgagcttg gcgagaccag ccggaaagtc accagcatcc gggtcagcag
     2341 ccgcagcgcg gggtcgattc cgtcggagac caggccttcc gccgccagcg ccgccaaggc
     2401 gtcctccagc cgcggagaaa ggccgacgcg gtggcgcagc tgcagggtat ggaccgcgaa
     2461 ttccaggtcg acgaggccgc cgtcgccgag cttgatgtcg aacggaccgc caggcggctt
     2521 gtgcgcggcg atgtcggccc gcatcctggc cgcgtcggcg gtgaccttcg ccggatcgcg
     2581 cggcatcgcc agcgtggctt ccacgatccg cgcgaggctc gcccgcgcct ccggcgagcc
     2641 gtagaccggc cgggcccggg tcagcgccat atgctcccag gtccaggcgc gctcccgctg
     2701 atattcggcg aagctgggca gcgagatcgc cagcagcccg tccttgcccg agggccggag
     2761 gcgcgtgtcg acctcgtaga gcggcccggc ggcggtcgcg acgctgagcg cggcggtgac
     2821 gcggggcgcg agccggttga aataatcgga ggcgcgaagc ggcttgcggc cgtccgactc
     2881 ggcctcgtgg gtgccgctga acagatagat gagatcgagg tcggaggcat gagtgagcgc
     2941 ttccccgcca agccggccga ggccgaggat gacgagctcg gagcccggga cgcggccatg
     3001 ggcccgctcg aattcggcga cggcggcgtc ggcaagcacg ttgatcgccg cctcggcgac
     3061 gcgcgaatag ccctccgcca cttcgagcgg atcgcggcgc gccaccacca gctggacgcc
     3121 gagggcgaac cggcgctcgt tcacccggcg gcggacgcgg tccagcacca gctgataatc
     3181 ttcgccggcg cgctcggagc gggcgaaatc gcggatcagc tgcgccgccg gcggcgccgg
     3241 ctggaaggcg ctggcgtcga tcaggccgtc cagcaattgc ggccgccgcc cgagctgttc
     3301 tgccagagcc ggggcatggc tgagcaccgt cgcgagcaac tgcgccaatc cgggccgcgc
     3361 ctcgagcagc cggtagaaat tgaccccgct cggcagccgg ccgacgacgt cgtcgaatcg
     3421 gttcatcgcc cgcatgctgt cgggcgacgc gccgaaggcg tcgatcagca ccggcagcat
     3481 cgcttcgaac gcgccccgcg ccggcgcggt ccgcagcgag cgggcgcgtc ccgagcgcca
     3541 gctttcgatg cgcagccgcc cggcgacggg atcgtcgaaa ccggcccgcg ccagccgcgc
     3601 ttccaaagcc gcgctgtcgt gggggagccg gtcgccaccg ccttccccca attcgtcgta
     3661 gatcgccgcc acctccgcca ctggctccgc cagcaggtcg agcaaagcgg gcgcgccgtc
     3721 gaggccgtgg agctgagcca cattgtcgag cgcggcggcg ccggcgggca ggctgtgggt
     3781 ctgccggtcg tcgaccatct gcagccgatg ctccatcgtc cgcagcaacc ggtagaccct
     3841 ggcgagcgtc tcggcctggg ggaccggaat gcggccggcg gcggcgagcg cggaaagcgc
     3901 gttgagcgtc gccgccggcc gcagcccggg ttcgcgcccg ccgtggatca attggtgcat
     3961 ctgcgcgaaa aactcgatct cgcgtatccc gccgcgcccg cgcttgaggt cgaagccggg
     4021 accgaaccgc tggccctgcg cataatggtc gcggatccgc cgcgtgatcg agtggagctc
     4081 gccgatcgcg ccgaaatcga gcgagcggcg ccagacgaac gggtggatcg cctccatgaa
     4141 atagcggccg agcgcgggat ctccggccac cattcgggcg cggatgaagg ccgcccgctc
     4201 ccacggcagc gcgctcgatt cataatagct gatggcggca tcgaccggca gcgcgatcgg
     4261 cgtcacttcc gacgaggggc gcagccgcag gtcgacccgg aagacgtacc cttcgccgtc
     4321 ccttttctga aggaggtcga ccagccgctg gccgatcctg accgccgcct ggcccgcatc
     4381 ctcgcgcggc ttcagcggca gcgtcgttgg atcgaacagg aagaggacgt cgatgtccga
     4441 cgaataattg agctcgcggc tgccatgctt gccgagcgcg atcaccgcga agccggtcgg
     4501 gctcgcgcca ggcgtacggg cctcgataat ggccgacagc gcctgctcga gcgcgaggtc
     4561 ggcgaggtcg gaaagcgcct cgaccacctg ctccagcgtc agcgcgccgg cgaggtcgcc
     4621 gatgccgagc gccagcgcgg tcgcgctgcg ctcccgccgc agcgcggcgg ccaagtccgg
     4681 cgcgccatcg cccgccgtcc gggccgccgc cagcgccgca tcgaccgagc ctccggccag
     4741 cgcctcggcg accgccggga acgcatcgag ctgcaatttc aggaacggcg aatgcgcccg
     4801 cgcgcggcgg agcgcgtcgt caatgtcccg gtaaccctct gtctccacca tgccacaaaa
     4861 cagagcagtt ccgttcttgc cgcgcaacat ttgcaaccgc gatggcccgc gaacgttcta
     4921 acctgtgtga atatgatgaa cccgagaaga gaagcgatgg tcaatgcttc ctcacgccgc
     4981 ttcgtgaaag tggcgccgcc gacggtccat accggagtgg gcaatgcctt gcgcgaagcc
     5041 ttcgcgatgg acggacaggt gcgctcgctg cagatgttcg aggatctgct cgcgaagctc
     5101 gactgacctc aatcctgcgg gtcgcggtcc cggctaaggt cgtcgacctc actcatgatc
     5161 gactgcagcg ccgtcttgtt ggcgtcggtc tcatggtccc ggcgcgaggg cagcttgcct
     5221 tcggcgagca gggtctcgag cgcgacccgg ccgcgggcca cccggctctt gatcgtgccg
     5281 accgcgcagc cgcagatttc ggcggcttcc tcataagcga acccgcccgc cccgaccagg
     5341 atcagcgctt cgcgctgcgg ctgcggcaag tgcatcagcg cgcgctgcag gtcgccgagc
     5401 tcgacatgac gatcctggct ggcgggcgcg gcgagcagct tcgaggcggt caattcgtcc
     5461 cactcgccct tgaagcgggc gcggcgcatc tggctcaaga agaggttcct gaggatgatg
     5521 aaggtccagg cgcgcatgtt ggtgccggcc tggaagcgtt tgcgggccgc ccaggccttg
     5581 agcaaggtct cctggacgag gtcgtcggcg aggtcgcggc tgcccgacag cgagcggccg
     5641 aaggcgcgca gatgggggat gacctggccg agctgatcct tgaactcatt gtccggcagc
     5701 ggcaccggct cttcgcgcgg cacctcggct acatcgtcct cgtcgatcaa cgctggcttc
     5761 tcgcccgcca ttcaactgcc ccaaaaatgg tggttatgcc ctcgcgaaac cggacgccaa
     5821 cgccaggcag ccgcccgata atgtttcgga aatgtaagag gctagcgcca gacaaacaag
     5881 atgagcgcga acaggatgat gaccagcacc agcccgatcg acaggacgta gcgcgtcatg
     5941 tgcggcgtcg tgccgccgcg cgcctcggtc ttgccaatgt gggggctgcc gtcatccatg
     6001 gggtttgcgc atcctgaact aactggaact atccgggcgc aacgcccgct gcggacgccg
     6061 gttccggact ttctttacgc ggccggaacc gtcgcgctgt cgaagaacaa agcctgggcg
     6121 atggcggcct ttacggtcga gcgctggaac ggcttggtga tgaggaaggt cggctccggc
     6181 cgctccccgg tcaacaggcg ctcgggaaag gcggtgatga agatcaccgg aaccgagaat
     6241 tcctggagga tgtccttgac cgcgtcgatg cccgagctgt cgtcggcgag ctggatgtcg
     6301 gcgaggacca ggcccggccg gcgcgccatc gcctggctga ccgcctcgtc gcgagtgacg
     6361 gcgacgccgg tgacggtgtg gccgaggtcg cggacgatcg tctcgatatc catcgcgatg
     6421 atcggctcgt cctcgatgat cagcacttcg gcccgggtct ggcgctcgat ctcggcgagc
     6481 gcttcggcga caaggttgtc gacctcctcg gccgaggcgc cgatcagata gccggtatcc
     6541 tcgggcgtga agccctccat cgcggtcagc agcagcgcct ggcgggacag cggcgtgatc
     6601 cgcgccagcc gcgcctgggc gatgccctcc gcctcgctgg cgtccagcga gctcggatcg
     6661 tcctcgatat tggccgacga ccagattgcg tggaacgtgc ggtagaggcc gagccgggca
     6721 tcgacgtcgc gaggaaaatc gtcgggcgcg gcgacgatcg cctcgagggt cgcccgcacg
     6781 aacgcgtcgc catgcgcctg cgtgccggtg agcgcgcggg cgtaacggcg gacgaaggga
     6841 aggtggggcg cgagttcctg accgagcgac atgaccgtta cgactcctat gcgatacttg
     6901 aggggccttc tgattggctg gaaggcgctt ggcaagccca gccggacacc ccccggaggg
     6961 gcggacaggc tggttcggaa tcgtccgggc ccgctcctct cgaaaggctg gaaccattaa
     7021 gcgagttttt tgtttccatg ccgatccggg ccttataggt ggcgggttgc tggggtctgt
     7081 ccagtctcgg cgggaggcag ccgaggccca cggcgggtcc ctattttcac gatggagcgg
     7141 gcgcggcgac ggccgcgagg ggtatacaga atttgacctt cgcacatgac aaggatgccg
     7201 gtagcgggcg caagcccgat cgcgccgccg cgtccgatgc cggcgaacgg acgccgccga
     7261 aaggccgcaa gcgcccggtc acgcccgagc tcggacatgc gctgaggaac gcttaccagc
     7321 gcacgatcga cgaagacatt ccgcccgaga tgctcgatct tttggggaag ctcggataaa
     7381 gccccaccga cagacgttcg gaaccccgtg agccaagaac aaccgagcca cgaagaacaa
     7441 ggctggttcg catcgcgctt cgcgcggcta tcgaccgggc tgaaaatgtt cctcatcctc
     7501 agcctggcgc tgtttccgct ggggctgatc gccatccttg cctcattgga aagcgcccgc
     7561 gacaacagcg ccggccgcgc ccagcacata ttcggccggc tggaggtcaa ggcgcagcgg
     7621 atcaactcgt tgctgtcgcg aagctccggc accatccgcg ccgccagcgc cgcgatcgcc
     7681 gtcagccccg ggtcggacgt ctgccgcgtc accctcgccc ggctcgcccg cgcccaagtc
     7741 accaccggcc gctacgccct ttacggcggc ggcccgcggc cgcgctgcgc gactcccggc
     7801 ttcgcgcccg cgccgatcgc caatccggag cgccggccct cgattatcga gatcagcccc
     7861 gacggcagga tcctgcgctt cgcgctctat gacgacgccg acaatccgga gggcatgggc
     7921 gaattcaccc gcgagagcct ggccgagctc accctgctcc ccggcacccg cggcaatttc
     7981 gacctcgagc tgctgcaggg cggccgcggc atgatgctgc ggaacggcta tcagggcggc
     8041 ccgctggtcc agagcgtcac cggcaccgag ccggttgcgg gcgaccgtct ccagctccgc
     8101 atccgccaaa gcgcggtgcc gatcagcccg gtcgagctgc tgatgatcct gctgccggtg
     8161 atgatgtggc tgggcgccgg catcatcggc tggctgatcc tcgaccggct gctgctgaag
     8221 ccgctggtgc ggatgcagcg cgccgtcgcc gcctaccggc ccggcgacca gcatctcgac
     8281 ctgcccgcaa tctcctcccc cgcccgcgag atcggcgagc tcggcgccgc cttcgaccgc
     8341 gtcacccaga ccgtcgcccg ccacgaagcc gagctcgaag cggcggtcga gcgccagcgg
     8401 cggctggtgc gcgaagtcca ccaccgggtg aagaacaacc tccaggtggt ggcgtcgctg
     8461 ctcaacctcc acgcccgcgg cgtcgccaac gaggaggtcg ccgccgctta tgcctcgatc
     8521 cagcgccggg tcgacgcgct cgccgtcgtg catcgcaacc attatgcgga gctcgaggag
     8581 aatcgggggg tcgcgctgcg ctcgcttatc tccgagctcg gcgccaacct tcgcgccacc
     8641 gcccccgcct cggccggcgc gatggcgatc cggatcgaca tcgagcctta ttacgcgacc
     8701 caggacgtcg ccgtctcggt cgcctttctg atcaccgagc tggtcgaata tgcgatgttc
     8761 tgcggcgccc gcgcggtcag catctcgctc accggcggcc gcggcccggc ggccttgctg
     8821 gcggtggaat cggacagcct catcgccggg gccgaatgcg cgcccgaact caccgagcgt
     8881 ttcgagcgga tcgtcaccgg cctgtcgcga cagctccgct cgacgctcga ccgcgacgcc
     8941 gagcgcggcc gctacgcgct cgagatcgcc gtcgtcgaca aggccgagcg ctgaggcagg
     9001 gtgagccgct gtaagcggcg gtcgatccta tcaacccgtc gcccccgact gatcgggggt
     9061 gtcccttcgt caacggcccg aaggaaggca gatgccggat caagtccggc atgacgaaca
     9121 gggcggctag cccgagctgt cctccgggcg gtcgagatcg tcggcgcgcg gcgggcgtcc
     9181 gaccgagcgg cgcttggcgt cttcgaccgt ccccgcgctc tgccccagat catgggcggt
     9241 atcgacgttg cggcgctcgt cctggtggac gccgtcaatg tcggaatggg gcgtggattg
     9301 cggcggtttg gccatcggtt tcggttcctt tccccggctg aaccagccga tccggcggcg
     9361 gttgcgcctg ggtcggttat gccccggact ttttttgata ataagcgatt tttttcagct
     9421 cgtcgcggaa cccccgcccg gcttcgtcgt tctgctcttc ggatagtgac cttttgcccc
     9481 cccgctctcg ctatccagaa catgagcccg gacgcgccaa cctccccccc ccccggttgc
     9541 gcctccgggc tcaacatttt tgagctcccc cctccatcgt tgcatctccc ccgcatattt
     9601 ttgcgcggcg gctcttccgg tcgcgccgcg cttgctgcta acgtgacgga gacgaaacga
     9661 tgcgcccgtc acgcgggggg aaccaagccc ggcccggacg catttacccg tcgcctcgcg
     9721 cgccggtttc cggcgtccgc gctcgaacgt caggagacag acatgcgtaa gattctcgcg
     9781 attttcgccc tttccacgag cctcgcgctc gccgcctgca acaccgtcga aggcgtcggc
     9841 gaagacgtcc agtcggccgg cgaagcggtc gaagacgccg ccgattgagc ccatttcctt
     9901 tcggaaatcg gaaaggcgtc gcccccccgg ggcggcgcct ttttcgttgc cgcgcgcccc
     9961 ggcgctcaga cgacgaattc ggccgccgtc agcgcgatcg ccccgtcgag ccggatcatg
    10021 aagtccgctt tgccgtcgcc gtcgacgtcg ccgctcacca aagtcgcgcc attggcgtga
    10081 tgccagcgca attcgccggc cgtgcccgaa aaggcagcct cgccgacgaa ggtgaagcga
    10141 tcgtccgcgc cgccggcgac cgcatcgacc gcgcgaaggt tgatcctgtc ggccgccgcc
    10201 tcgaacccgg ctatccggtc ggcgctacgg gcgacggcgc cgccgaaatc cccgttcgcg
    10261 aacaggaact ggtcggcccc cgctccgccg agggcgaaat cgcgcccggc gccgccagcc
    10321 aggcgatccg ctccggcgcc gccggccaac cggtccgccc catcgaggcc tgagagaaga
    10381 ttggcgccgg catttccggt caatgcgtcg gcgaaggctg agccttcgag acgctcgacg
    10441 ccgagcaacg tgtccaggcc cgccagcgtg tcctgcgccc ccgcaacggc aaggctggcg
    10501 cgcacgcccg ccgcggcgga cctgaaggac gcagtgtcga gaccggcgcc tccatccagg
    10561 cgatcgtcgc cgccgccgcc ttcgagccgg tcggcgccgc tgccgccgtc caggcggttg
    10621 gccccgccgt cgcccgtcag cgcgtcggca aaggacgacc cgatcagggc ctcgacctca
    10681 ctcatatttt cccagcgctg aagcgcctcg atgtagcggc cctggttgag agccacggtc
    10741 acgccgcggg cctcgccttg gtaactgacg gtgtcgaacc cgtcatggcc ctggtaggtg
    10801 tccgcgccct cgccgccggc cagcaggtcg tcgccgacgc cgccgttcag cagatccgcg
    10861 ccgagcccgc cgctcagcgc gtccgcgtcc cagccgccat aaagttggtc ggcatggtcc
    10921 ccgccgtcca gcacatcgtc gccccagccg ccgctgagct cgtcgcggcc ggcgccgcca
    10981 gcgagccggt cggcgccttc gcggccgttg agaaaatcgt cgccgccgcg gccgtcgatc
    11041 gagtcgcgcc cgccaatgcc gagcaggatg tcggcggtct cggacccggt ctggctgagc
    11101 cgccgcgccg ccgggtccca cgaccagctt gccgcctgcc cgccgtcggc atcggcgtcg
    11161 ccgtagagga actggacggc gtcccggtcg agcggcccga gcacgtctcc cggaaagccg
    11221 tccgaggaat agctcatcac cgtctcgcca taatggtcgc ggtccgggtc cagagtgggc
    11281 gagccctcga acggatgctt gaggccgatg gcgtggccga tctcgtgaag ctggatgtga
    11341 agatcgtcgg cccagcgaaa atcgaggaac acgtcgctct ggaagccgcc gtcgccatat
    11401 tggccgcccg gcagataggc gaaggcggcg tcgaactggt ccggcgtcac gagagcgagg
    11461 tcgaacgaca tgaacttgat gtcgccgtcg ccgggagcga cttcgaaaaa ggtgaggccg
    11521 caagcctcgc cccaggcggc gatcgcctcc cgggcgaccg ctttttccgc ctgcgtcatc
    11581 gggcggaagc ttgccagccc ttcctccgga tagactccct cataataatc ggggacgctg
    11641 gtcgggaagg agaagctgca aaaggcgccg atcccggagc ggccgtgcag cgacgagccg
    11701 gagatgaggg cggtatagtc gctgaccgtg gcctcgttcg cctcggcgcg ggcgaacgcc
    11761 tcaccttcga tccgcgctgt gcggccccat ggctcgaact ggtctcgcat ggcgcccttt
    11821 cagtcctgcg gtgcccgata ttgcccgctt ccccggctcg cggctagacc agatcgtcgg
    11881 ggatgaacca ataaggccgc gcgaagcgac gggcgcgtcc gagctggtag ggaagaacga
    11941 agccgcggcg ctcgtgcggc tcggccgcgc tgttggcgcg agcggcgtcg cgccagcctt
    12001 ccagatccat cgaatcgggc agcgccccgc cgaacagctg ccaccacaaa cgccgcttga
    12061 actcgaagat cgcctcgcct tcttcggtcc acaggaagcc gagctcggta tcccattcga
    12121 aacttcggcc gttgatgttg gccgacgaga gcaggcaggt cgcatcgtcg gcgatcagca
    12181 gcttggaatg gatgtggatc agccccgatc cgaaggcggt gccgcgggtc tcctcaaacc
    12241 cctcctcgct gcgtttcacc gcctcgtgct tggcgagcgt gaacagcccg acccgctgcg
    12301 ggcccgcttt ttcaagcagc cgcccaagcg cgcgcgcctg cagatattcg ccgtggcgat
    12361 gggcgagatt gtcggtctgg ccctcgaagg cgatctcctc gggcgtgttg gcgacgagga
    12421 tgatgacttc gagctcggcg gttgagctca gcgcctcctc gatccagtcg gcggccgcct
    12481 cggagcggaa gaattgcgct tcgacgtaga gctgccgctt ggccgagagg atcagctccc
    12541 ggtgcgcggc cttgagctcg cgaatgccgt gcaccggtcc ggttgcgaag aaagaggcgg
    12601 atttgcgcga gagcgtccgg accagctgga ccttggcctc ccctgcccgc tccggctcgg
    12661 cgatgccctc gatgggcacc tcggagagcg gatcaagcat cagccggcgg ccgcactcgc
    12721 cggtccattc gccgacggtg tcgcggtagc gcggcatttc ccggttccac agccgcgcga
    12781 aatggcgggc ggcgtcggcg accaccggcc cttcgaccag cgccgaaatg tcgtgccagg
    12841 tctggtcggc gcgttgccgg tggcggcggt cgtcccagcg ccgttcgtcg aggtcgagcc
    12901 cgccgatgat cgccttttgc cggtccacca ccacgaactt ctggtgatag gtggccggcc
    12961 acagccgcgg cggcggcgcc ggcttccagc tcttgggctt gccctcgtgc caatcgacat
    13021 ggcgccacag gcccggccgc gtgtcgaagc cgccatcgtc atgctcgtcc ttggcgagca
    13081 gctcggccac caccttgcgg atgcggcggc gcagcagcag ccggagcaat tgccgccagc
    13141 cccagccgag ctcgccttca tgctggacga tcatcagctg gagccgctcg ggatggctgt
    13201 cgccaagctc ctcgaccagt tcgcggatgc ggcggtaggt gttccacgat ccggcatgga
    13261 gatggtcggc cagcaccggc tcgaaatcgg cgagcaggat gcggacctcg acgccgcggc
    13321 ggaccgcgtc gcggatcagt tgcgcccaat cctcgatgcc ggccgccacg gcgcgctcgg
    13381 agcgagtctt ggtgttgggg tcgaagacgc ggaaggagag ccacaccgat tgccgggcgt
    13441 cgagcacgag atgctcgagc atcggataca tgtcggcggc ttcgatgagc ggggtgacgc
    13501 ggcaaccttc gcgcggcggc ggcggatgcg tccagccggg cccgtcctcc accgcgagca
    13561 aggcgttgct ccgatccaga aaagggggct ccacatacat tgcgggaccg cgaggatcgg
    13621 cttcggccac tacaaaagct cactccctgt ggtctcgcgc gcccggcggc gctcggtgaa
    13681 ccagatcgca atcaacgaca gttcgtagag cacgatcagt ggcacggcga gcatgaactg
    13741 cgacagaacg tcgggcggag tggcgacggc cgcgatgatg aaggcgacga ggatggcgta
    13801 gcggcggccg cgcttcagct ggtcgcgggt gacgaggccg gcccgctcga gcagcatcag
    13861 cagcaccggc agcagaaagg cgatgccgaa ggcgaggatg aagtgcatca ccaggctgag
    13921 atattcgccg accgccggca gcgcctcctg ctcgatgccg ccgagattgc cctggaagct
    13981 caggaagaaa tgaaaggcgt agggcatcac cccgtaataa gccaaggccc cgccgagcgt
    14041 gaacagcacc ggcgtcgcga tcaggaacgg gaggaacgcc cgtttctcct tggagtagag
    14101 gccgggcgcg acgaacagcc agatctggtt ggcaatgacc ggaaaggcca ggaagaaagc
    14161 ggcgaacacc gccacccgca cctcgacgaa aaaggcctcg taaagcttgg tgtagacgag
    14221 cttgccctcg ccgggcggga acgccttgac gagcggctgg gcgagaaagg cgaaaatgcg
    14281 gtcggcgaaa tagaagcaga ccccgaacgc cgcaaacaag gcggcaaacg accacatcag
    14341 ccgccgcctg agctcgatca aatgctccat cagcggagcc ttggtgtcgt cgggctcgct
    14401 catggcagcg cccgatcgtc gccgtcggcg cgcggcggcg gcatctcgcc ctcgacggga
    14461 gcggg
//
LOCUS       DASVRK010000188         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_150191, whole genome shotgun sequence.
ACCESSION   DASVRK010000188 DASVRK010000000
VERSION     DASVRK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150191"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="VF589_07385"
     CDS             <1..103
                     /locus_tag="VF589_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX8257439.1"
                     /translation="HHKTEACFKAFGRALRQALARDGDALPSTKGVL"
     gene            complement(248..1906)
                     /gene="hisC"
                     /locus_tag="VF589_07390"
     CDS             complement(248..1906)
                     /gene="hisC"
                     /locus_tag="VF589_07390"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX8257440.1"
                     /translation="MSSLAARLARPEILALVPFDIAAQANDAFGPDAIKLDANENPFA
                     PLSEGALAAGLNRYPEPQPRRLKRALAALYGVTPDELAVTRGADDAIDILVRTFCRPG
                     LDAVSICSPTFSAYAHFAALQGARVVEARLDERFDFDADAFLAAVRSEGSLKLAFICS
                     PNNPTGNPVDPADILRVADALPDTILVLDEAYIEFSETPSLAAEAVRRPNLAVLKTLS
                     KAYGLAGARVGALIANPELVALAARALPPYPLPSLSVEAAMAALAPSRRPIHEERIAR
                     IKAERERLAEILAGSPLVTSVRNGGGNFLFLGVADPAALAEKLQALGIRARFRPNAAP
                     GGVRLTIGTEAENEAALAAFGVASHSAPARRGEIVRETKETRIAVAGDLDRPAPRRID
                     TGIAFYDHMLDQVAAHAGFSLILSCEGDLDIDAHHSLEDCAIAFGTALSRALEGRRGI
                     GRFGFSLPMDETEAHVLIDLSGRPYSVFDGSFEASHIGAYPTEMTRHVFRSLADSLGA
                     AIHVRVAGENDHHKTEACFKAFGRALRQAVARDGGGVPSTKGVL"
     gene            complement(1903..>2784)
                     /gene="hisD"
                     /locus_tag="VF589_07395"
     CDS             complement(1903..>2784)
                     /gene="hisD"
                     /locus_tag="VF589_07395"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX8257441.1"
                     /translation="PARAAGVSDIVVVTPPRPEGGLDPVLALAAELCGIEAVWTVGGA
                     QAIAALAFGAGGIGQVDKICGPGNAWVAEAKTFVASLAGGPAIDMPAGPSELLVIADD
                     GADPAMVAADLLGQAEHDASAQVLLVTPSAALAAAVEAELQSQLRTLPRAEIAAASLA
                     EARIILVADLEEAVAISNLYAPEHLSLAVAEPEALIGSVRNAGAVFAGRFAAETFGDY
                     LAGSSHVLPTDGAARAWSGVSVHTFLKAITVQTVTREAARRIAAPAAALARLEGLEAH
                     ARAADARAEAPAEKVPA"
BASE COUNT          410 a          971 c         1026 g          377 t
ORIGIN      
        1 ccaccacaag accgaagcct gcttcaaggc tttcggccgg gccctgcgcc aggcgcttgc
       61 ccgggacggc gatgccctgc cgagcacgaa gggcgttttg tgatgagatc ctccccggga
      121 cggggagggg gaccatgcgc agcatggtgg aggggggcga gcgcagcgag cggggaggcc
      181 gggggccccc tccaccaccg ccgctgcgcg gcggcgggcc ccctccccgt tccggggagg
      241 agcttgctca cagcacgccc ttggtgctcg ggacgccgcc gccgtcacgg gccaccgcct
      301 ggcgcagcgc gcggccgaac gccttgaagc aggcttcggt cttgtggtgg tcgttctcgc
      361 ccgcgacccg gacatggatg gcggcgccga ggctgtcggc gagcgagcgg aagacgtggc
      421 gggtcatctc ggtcggatag gcgccgatat ggctcgcctc gaagctgccg tcgaagacgc
      481 tgtagggtcg gccggagagg tcgatcagca catgcgcctc ggtctcgtcc atcggcagcg
      541 aaaatccgaa ccggccgatg ccgcggcggc cttccagcgc gcgcgacaag gcggtgccga
      601 acgcgatcgc gcaatcttcc aagctgtgat gggcatcgat gtcgaggtcg ccttcgcagg
      661 acaggatcag cgagaagccg gcatgcgccg ctacctggtc gagcatatgg tcgtaaaagg
      721 cgatgccggt gtcgatccgg cgcggggctg ggcggtcgag gtcgcccgcg accgcgatcc
      781 gggtctcctt ggtctcgcgc acgatttcgc cgcggcgggc gggggccgag tgggaggcga
      841 cgccgaacgc cgccagcgcg gcttcattct ccgcctcggt gccgatggta aggcggacgc
      901 cgccgggcgc cgcgttgggc cggaagcgcg cgcggatgcc gagcgcctgc agcttctccg
      961 ccagcgcggc cggatcggcg actcccagaa acaggaagtt gccgccgccg ttcctgacgc
     1021 tggtaaccag cggcgacccg gcgaggattt ccgccagccg ctcgcgctcc gccttgatgc
     1081 gcgcgatccg ctcttcatgg atcggccgcc gcgacggggc cagcgcggcc atcgccgcct
     1141 cgaccgacag gctgggcagc gggtagggcg gcagcgcgcg ggcggcgagc gcgaccagct
     1201 ccgggttggc gatcagcgcg ccgacgcgcg cgccggccag tccataggcc ttggagagcg
     1261 tcttgagcac cgcgaggttc ggccgccgga ccgcctcggc ggcgaggctc ggcgtctcgg
     1321 aaaattcgat ataggcctca tcgagcacca ggatcgtgtc cggcagcgcg tccgcgacgc
     1381 ggagtatgtc cgccggatcg accggattgc cggtcgggtt gttgggcgag cagataaagg
     1441 cgagcttcag cgagccttcg cttcgcaccg ccgccaggaa agcgtcggca tcgaaatcga
     1501 accgctcgtc cagccgcgcc tcgaccacgc gcgcgccctg cagcgccgcg aaatgggcat
     1561 aagccgagaa ggtcggcgag cagatcgata ccgcgtccag gccgggccgg cagaaggtgc
     1621 gcaccaatat gtcgatcgca tcgtcagcgc cgcgagtgac ggcaagttcg tccggcgtca
     1681 ccccgtaaag cgccgccagc gcacgcttca gccgccgcgg ctgcggctcg ggatagcggt
     1741 tgaggccggc ggcgagcgcg ccttcgctga gcggcgcgaa cgggttctcg ttggcgtcga
     1801 gcttgatcgc atcggggccg aacgcgtcgt tggcctgggc ggcgatgtcg aacgggacga
     1861 gcgccaggat ttcggggcgg gcgagccgcg cggccagcga gctcatgccg gcaccttctc
     1921 ggcgggggcc tcggctcgcg catcggccgc ccttgcatgc gcctccagcc cctcgagccg
     1981 ggcgagcgcg gccgccggcg ccgcgatccg gcgggcggcc tcgcgcgtca ccgtctggac
     2041 ggtgatcgcc ttcaaaaagg tgtggaccga gacgccgctc cacgcccggg cggcgccgtc
     2101 ggtgggaagc acatggctcg atccggccag ataatcgccg aacgtttccg ccgcgaaccg
     2161 cccagcgaac accgcgccgg cattgcggac gctcccgatc agcgcctccg gctcggcgac
     2221 ggcgagcgaa agatgctccg gcgcatacag gttggagatc gccaccgctt cttcgaggtc
     2281 ggcgacgagg atgattctcg cctcagccag cgaggccgcg gcgatctcgg cgcgggggag
     2341 ggtgcgtagc tggctttgca gctcggcctc gaccgcggcc gccagcgcgg ccgaaggcgt
     2401 gaccagcagc acctgcgcgg aggcgtcatg ctcggcttgg ccgaggagat cggcggcgac
     2461 catggcagga tcggcgccat cgtcggcgat caccagcaat tcgctcggcc ccgccggcat
     2521 gtcgatcgct gggccgcccg cgagcgaggc gacaaaggtc ttggcctcgg ccacccaggc
     2581 gttgccgggc ccgcaaatct tgtcgacctg gccgatcccc ccggcgccga aagcgagcgc
     2641 ggcaatcgcc tgcgcgccgc cgaccgtcca caccgcttcg atcccgcaca gctcggccgc
     2701 gagcgcgagt acggggtcga gcccgccctc cggccgcggc ggagtcacca ccacgatgtc
     2761 gctgacgccg gcggcgcggg cggg
//
LOCUS       DASVRK010000189         8961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_98298, whole genome shotgun sequence.
ACCESSION   DASVRK010000189 DASVRK010000000
VERSION     DASVRK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98298"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="VF589_07400"
     CDS             <1..1647
                     /locus_tag="VF589_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX8257442.1"
                     /translation="ALTYRKSRSVTTPEETVPAPPGPKKGALVRSTLVVTVVRGIDFV
                     LSFLVSVLLASRFGVSGQIDAFFLARRITVGFAETIRHLVNMVVVPAVVARQERGDQP
                     GEHGLPRQFYLFLGFIALLTLVGTFAPGPLVAFFAPGFDGERFDLTSRLTSIMMPLLA
                     IAIVSSLLMALLQANRRFFSAEASKTLQRALLVAMLFFLIPPLGIFDAAWTMLIGGVV
                     GLVYLTAVSWRFLRADPSRLLTAKAKKPSADRARGGGLVAAIILQLYYVATSLVDFAV
                     ASTLDVGSVSALEYGTRLVSILPGLVTASVYTVIYPELIRAMHDRDPIRAAAKIGEYQ
                     RLTLFIQMPVSIGLMLTAYPIIDIIFGHGQFGQANVAVTAAVTVGYAAAAIFLMPFHI
                     ATSAIYADPARSPAVDIAIICVGGFAVRAALVAAAAPFGAEGIAWAATLATALTLALA
                     MVVARRRFPHFSLAAQARDFASTGLCGLAAAAAGILLLQFLPSDVGVVDGLAVIALVG
                     SAALAVYAAAGWLLRVPELREASALVAARFGRARKKGEGA"
     gene            1644..2846
                     /locus_tag="VF589_07405"
     CDS             1644..2846
                     /locus_tag="VF589_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HEX8257443.1"
                     /translation="MTRLPSALVAGVDRPASLAVVRELGRRGVPVHGVGRDLQAVGRT
                     SRYLQRFALRPAGPITAWLPGLMAEWGSEVALGLGEEDLLALRAAGGSIPGCRILAPP
                     LEAFAKVVDKPRTLQVAESLGIDVPRTWQPRSPDEPMPDLGWPVVVKWPNPAVAKPAL
                     AAAGLPFLKTDYCAGEAALRRLLASYAPVGSLPIVQQRVAGHGLGHMLYLHEGKAVLR
                     FQHERLHEWPAEGGVSTLCRALPLSRHAAQMDKSEALLAALGWEGPAMVEYRHDPATG
                     RFVLMEVNGRFWGSQPLATACGAEFAWELYRRQMLGEDGPAPAPRTNLRARHMMRESK
                     RLVRLLAGRGKSRDPAFHPTPLADLARYLAGFVDPRTRYYVLSLDDPRPFLTDLAETG
                     RRLARKLS"
     gene            2909..5203
                     /locus_tag="VF589_07410"
     CDS             2909..5203
                     /locus_tag="VF589_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX8257444.1"
                     /translation="MRIACLGGGPAGLYFAISMKLRDPAHEVDLFERNRADDTFGWGV
                     VFSDQTVENLMANDPVSGAVIQGEFAHWDDIDVHIHGSAIRSSGHGFIGIGRKRLLAI
                     LQQRARELGVRLHFEHEASPDLADWSDYDLVIAADGSNSRIRTRYEPHFGVDIQVRRN
                     KFFWFGTAKVFEAFTFAFEQTEAGWVWAHAYRFGDGLSTFIVEMDPETWAGLGFDRMD
                     QPEAIALCERIFARYLDGNPLMSNAAHLKGPEAWLNFRRIICDRWSYGNLILLGDAAH
                     TAHFSIGSGTKLALEDAIKLAEVLNRPGLSRERALEEYREERNLEVLKLQNSARNSTE
                     WFETLGRYLHFEPIQFAYSLLTRSQRVSHENLRLRDRSWLEGVERWFQSQARGTPVND
                     PAPPMFAPYRLRGMALENRVVVSPMATYSATDGTPNDFHLVHYGTRAQGGAGLVYTEM
                     TCVSPTARITPGCTGMWSDEHVGAWRRIVDFVHANSAAKICLQLGHSGAKGSTRVGWE
                     GYDVPLEAGNWPLIAASDVPWSPANQVPRPMTRADMDLVREQFVAAVRMGLEAGFDMV
                     ELHSAHGYLLSSFITPLHNNRTDQYGGSLANRLRYPLEIFEAMRAAWPSDRPMSVRIS
                     ATDWMGELGIGPADAVEIARAFAEAGADLIDVSAGQTWADSAPVYGRMFQTPFADRIR
                     NEARVSTMAVGNIYETDHANSILAAGRADLVALARPHLIDPMWTLRAAAQQQYRGVDV
                     PPPYLGGMRQLARNFAREAEMLKA"
     gene            5227..6045
                     /locus_tag="VF589_07415"
     CDS             5227..6045
                     /locus_tag="VF589_07415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8257445.1"
                     /translation="MTGGGTGIGAAVARALAGEGAKLTLVSRRRDKLEEVAEEIRSSR
                     AKSRDPIERSRDGSADESLGSARRGVSTSLDTNGIFIAPADVTIREEVEAAFASAREA
                     NGPITILVNNAGAAAGVAFSKVTDSLWRDMLAVNLDGMFHCCQAALPDLLAAEAGRIV
                     TIASMAGLHGFPYAAPYIAAKHGAVGLTRALAAEYERTPLRVNAVCPGFVDSDMTVQS
                     ISNIKAKTGRSDDEARTELARLNPSGRLITPDEVAAEALDLILSQRNGEAVEIA"
     gene            6042..6851
                     /locus_tag="VF589_07420"
     CDS             6042..6851
                     /locus_tag="VF589_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX8257446.1"
                     /translation="MNPQTFEPEHFGWRFADGVATITLDRPERKNPLTFQSYAELRDT
                     FRDLVYEREVRAVVVTGAGGNFSSGGDVHEIIGPLTGMEMPGLLAFTRMTGDLVKAMR
                     ACPQPIVAALDGICVGAGAILAMASDIRLATPATKTAFLFTRVGLAGCDMGACAILPR
                     IIGQGRASELLFTGRVMTAEEGERWGFHNRLVAVDDILDEAQDLAHALATGPTFAHGI
                     TKTQLNTEWAVSIDVAIEMEAQAQAICMATNDFRRAYEAFADKQTPEFQGD"
     gene            6891..8057
                     /locus_tag="VF589_07425"
     CDS             6891..8057
                     /locus_tag="VF589_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8257447.1"
                     /translation="MSDRSFLDWPFFDDSHRALAEALELWCEAELSDEEPEDVDDACR
                     TLVAKLGAGGWLRYCVPAAYGGAHEALDVRSLALIRETLARHSGLADYAFAMQGLGSG
                     TITLFGSEQQKQAYLPAVARGEKIAAFALTEPGSGSDVAAMETAARLSEGGYVVDGAK
                     TYISNGGIADYYVLFARTGEAPGARGVSAFLVDRGAEGLDESERIRVIAPHPLATLRF
                     HGMRLPAEALLGTSGQGFAQAMATLDIFRTTVGAAALGFARRALDEATGRALARRLGN
                     GALADNPMIQAMLAEMVLDVDASALLVYRAAWLRDVRGERNSREAALAKLHATDSAQK
                     VIDKAVQIFGGLGVTHGVPVERLYREIRALRIYEGASEVQKVVIARRHLAGYSA"
     gene            8054..8446
                     /locus_tag="VF589_07430"
     CDS             8054..8446
                     /locus_tag="VF589_07430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8257448.1"
                     /translation="MKTLLPPGWPRPRGYANGISARGRTIFTAGVVGWDEEERFAAQD
                     LVGQFRQILRNTLAILAEGGAAPEHIVRMTWYVTDRSDYIANLREIGAVYRELIGKHY
                     PAMAVVIVAGLVEPAARIEIETTAMVPE"
     gene            8443..>8961
                     /locus_tag="VF589_07435"
     CDS             8443..>8961
                     /locus_tag="VF589_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8257449.1"
                     /translation="MILPYDSFVRDRLPPPEAQPDFLFDLPELAYPDRLNAAVELLRA
                     GPGEAPAVINDHGVWSYGELRDRSDRIARLLVEEEGLVPGNRVFLRGPNGAMLFAAWL
                     GILKAGGIVVATMPILRPGEIATILDRAQVSHAIVDSRFIGDFRQGAEQAGTVRSLIK
                     YDGDYGKGELESR"
BASE COUNT         1283 a         3341 c         2869 g         1468 t
ORIGIN      
        1 gcgcttacct accgcaaaag ccgaagcgtg acgacgccag aagagaccgt accggcgccg
       61 cccggtccca aaaagggagc attggtgcgc tcgaccctcg tcgtgacggt ggtccgcggc
      121 atcgacttcg tgctgagctt cttggtttcg gtcctgctcg ccagccgctt cggcgtcagc
      181 ggccagatcg acgccttctt cctcgcgcgc cgcatcaccg tgggtttcgc cgagacgatc
      241 cgccatctcg tcaacatggt cgtggtcccc gccgtcgtcg cccggcagga acgcggcgac
      301 cagcccggcg agcacggcct gccgcgccaa ttctatttgt tcctcggctt cattgccttg
      361 ctcaccttgg tcggcacctt cgcgccgggg ccgctggtcg ccttcttcgc cccgggcttc
      421 gacggcgagc ggttcgatct gacctcgcgc cttacgtcga tcatgatgcc gctgctcgcc
      481 atcgccatcg tcagctcgct gctgatggct ttgctccagg ccaatcgccg cttcttcagc
      541 gccgaagcca gcaagaccct gcagcgcgcc ctgctggtcg caatgctgtt cttcctgatc
      601 ccgccgctcg gcatcttcga cgccgcgtgg acgatgctga tcggcggcgt cgtgggcctg
      661 gtctatctca ccgccgtctc gtggcgcttc ctgcgcgccg acccgtcgcg gctgctgacc
      721 gccaaggcga agaagcccag cgccgatcgc gcgcgcggcg gcggtctggt cgcggcgatc
      781 atcctccagc tttattatgt cgccaccagc ctggtcgact tcgccgtcgc ctcgacgctc
      841 gacgtcggca gcgtctccgc gctcgaatat ggcacccgcc tggtcagcat cctccccggc
      901 ctcgtcaccg ccagcgtcta taccgtcatc tatcccgagc tgatccgcgc gatgcacgac
      961 cgcgacccca ttcgcgcggc cgccaagatc ggggaatatc agcggctcac cctgttcatc
     1021 cagatgccgg tctcgatcgg gctgatgcta accgcttacc caatcatcga catcatcttc
     1081 ggccatggcc agttcggcca ggccaatgtc gccgtcaccg ccgcggtcac ggtcggctat
     1141 gccgccgccg ccatcttcct gatgcccttc cacatcgcca cctccgccat ctacgccgat
     1201 ccggcgcgct cgcccgctgt cgacatcgcc atcatctgcg tcggcggctt tgccgtccgt
     1261 gcggcgctgg tcgccgccgc cgcgccgttc ggagccgagg gtatcgcctg ggcggcgacc
     1321 ctcgccaccg ccttgacgct ggcgctggcg atggtcgtcg cccgccgccg ctttccccat
     1381 ttcagtctcg ctgcccaggc gcgggatttc gcctccacgg gcctgtgcgg cctcgccgcc
     1441 gccgcggccg gcatcctcct gctgcagttc ctgccgagcg acgtcggcgt cgtcgacggg
     1501 cttgccgtga tcgcgctcgt cggcagcgcc gcccttgccg tttatgccgc cgccggctgg
     1561 ctgctgcgcg tcccggagct gcgcgaggcg tccgccttgg tcgccgcccg cttcggtcgc
     1621 gctcggaaga agggggaggg ggcgtgaccc gcttgccctc ggcgctggtg gccggcgtcg
     1681 accgccccgc ctcgctggcc gtggtgcgcg agctcggccg ccgcggcgtg cccgtccacg
     1741 gcgtcggccg cgacctgcag gcggtcggcc gcacctcgcg ctatctgcag cgcttcgcgc
     1801 tgcggccggc ggggccgatc acggcctggc tgcccggcct gatggcggaa tggggctcgg
     1861 aggtcgcgct cggcctcggc gaggaggatc tgctcgcgct ccgcgcggcc ggcgggtcga
     1921 tccccggctg ccgcatcctc gcgccgccgc tggaggcttt cgcgaaggtg gtcgacaagc
     1981 cccgcaccct ccaagtcgcg gagtcgctcg gcatcgacgt gccgcgaacc tggcagccgc
     2041 gctcgcccga cgagccgatg ccggacctcg gctggccggt ggtggtcaaa tggcccaatc
     2101 cggcggtggc gaagcccgcg ctcgccgcgg ccgggctccc tttcctcaag accgattatt
     2161 gcgccggcga ggccgccctt cgccgcctgc tggcgagcta cgcgccggtc ggttcgctgc
     2221 cgatcgtcca gcagcgcgtt gccgggcacg ggctcggcca catgctctat ttgcacgagg
     2281 gcaaggccgt gctccgcttc cagcatgagc ggctccacga atggccggcc gagggcggcg
     2341 tctccaccct ctgccgcgcg cttccgctca gccgccacgc cgcgcagatg gacaaatccg
     2401 aagccctgct cgccgcgctc ggctgggagg ggccggcgat ggtcgaatat cgccacgatc
     2461 ccgccaccgg gcggttcgtg ctgatggagg tgaacggccg cttctggggc tcgcagccgc
     2521 tcgccaccgc ctgcggcgcc gaattcgcct gggagctcta ccgccgccag atgctgggcg
     2581 aggacggccc cgcgcccgcg ccgcgaacca acctgcgcgc ccgccacatg atgcgtgaga
     2641 gcaagcgcct cgtccggctc ctcgccggcc gcggcaagtc cagggacccg gccttccatc
     2701 ccaccccgct tgccgacctc gcccgctacc tcgcgggctt cgtcgatccg cgcacgcgtt
     2761 attatgtgct cagcctcgac gatccccggc cgttcctgac cgacctcgct gagaccggcc
     2821 gccgcctcgc gcgcaagctc agctgacccg ctccgccctt gcctcccacg cccgccgccc
     2881 ctagccttgg gcgccaaggg agagcatgat gcggatcgcg tgcctgggcg gcggccccgc
     2941 cgggctctat ttcgccattt cgatgaagct gcgcgatccc gcgcacgagg tcgacctttt
     3001 cgagcgcaac cgcgccgacg acacgttcgg ctggggggtc gtcttctccg accagacggt
     3061 cgagaatctg atggccaacg atccggtctc cggcgccgtc atccagggcg aattcgccca
     3121 ttgggacgat atcgacgtcc atatccacgg cagcgccatc cgctcgtcgg gccacggctt
     3181 catcggcatc ggccgcaagc ggctgctcgc catccttcag caacgcgccc gcgaattggg
     3241 ggtgaggctc catttcgagc atgaggcgag ccccgacctc gccgattggt cggattacga
     3301 tctcgtcatc gccgccgacg gctccaacag ccgcatccgc acccgctacg agccgcattt
     3361 cggggtcgac atccaggtcc gccgcaacaa attcttctgg ttcggcacgg ccaaggtgtt
     3421 cgaggctttc accttcgcct tcgagcagac cgaggccggc tgggtgtggg cgcacgccta
     3481 tcgcttcggc gacggcctct cgaccttcat cgtcgaaatg gatccggaga cttgggctgg
     3541 cctcgggttc gaccgcatgg accagccgga ggcgatcgcg ctgtgcgagc ggatcttcgc
     3601 gcgctacctc gacggcaatc cgctcatgtc caacgccgcc catctgaagg ggccggaggc
     3661 gtggctgaac ttccgccgca tcatctgcga ccgctggtcg tacgggaatc tgatcctgct
     3721 cggcgatgct gcccataccg cccatttctc gatcgggtcc ggcaccaagc tcgcgctcga
     3781 ggatgcgatc aagctcgccg aggtgctgaa ccgccccggt ctctcccgcg agcgcgcgct
     3841 cgaagaatat cgcgaggagc gcaacctgga agtgctgaag ctccagaaca gcgcccgcaa
     3901 ctcgaccgaa tggttcgaga cgctcggccg ctacctccat ttcgagccga tccagttcgc
     3961 ttattcgctg ctcacccgct cgcagcgcgt cagccacgag aatctgcggc ttcgcgaccg
     4021 ctcgtggctg gagggggtcg agcgctggtt ccagtcgcag gcgcggggaa cgccggtcaa
     4081 cgatcccgcg ccgccgatgt tcgcgcccta ccggctgcgc ggcatggcgc tggagaaccg
     4141 cgtcgtggtc tcgccgatgg ccacctattc ggcgaccgac ggcacgccca acgatttcca
     4201 cctcgtccat tacggcaccc gcgcccaagg cggcgccggc ctggtttaca ccgagatgac
     4261 ctgcgtctct ccgaccgcgc gcatcacgcc cggctgcacc ggcatgtggt cggacgagca
     4321 tgtcggggcc tggcggcgga tcgtcgattt cgtccacgcc aattcggccg cgaaaatctg
     4381 cctgcagctc ggccattcgg gtgccaaagg cagcaccagg gtcggctggg aaggctatga
     4441 cgtgccgctc gaagccggca attggccgct catcgccgcc tccgacgtgc cctggtcgcc
     4501 cgccaaccag gtgccgcgcc cgatgacccg cgccgatatg gacctggtcc gcgagcagtt
     4561 cgtggcggcg gtgcggatgg gcctcgaggc cggcttcgac atggtcgagc tccactcggc
     4621 gcacggctat ctgctgtcga gcttcatcac tccgctccac aacaatcgca ccgaccaata
     4681 tggcggcagc ctcgccaacc gcctccgcta tccgctcgaa atattcgagg cgatgcgcgc
     4741 cgcctggcct tccgaccggc cgatgtcggt ccgcatctcg gcgaccgact ggatgggcga
     4801 gctcggcatc ggtcccgccg acgcggtcga gattgcccgc gccttcgccg aggccggcgc
     4861 cgacctcatc gacgtctcgg ccggccagac ctgggcggat agcgcgcccg tctacggccg
     4921 catgttccag acgccgttcg ccgaccgcat ccgcaacgag gcgcgggtgt cgaccatggc
     4981 ggtcggcaac atctacgaga ccgatcacgc caattcgatc ctcgccgccg gccgcgccga
     5041 tctggtcgcg ctcgcccggc cgcatctgat cgacccgatg tggacgctgc gcgccgccgc
     5101 ccagcagcaa tatcgcggcg tcgatgtgcc gcccccctat ctcggcggca tgagacagct
     5161 cgcccgcaat ttcgcgcgcg aggcggaaat gctgaaggcg tgaggctcct cggcgcccat
     5221 gcggtcgtga ccggcggcgg cacggggatc ggggcggcgg ttgcgcgggc gcttgcgggg
     5281 gaaggcgcga agctgaccct ggttagtcgc aggcgcgaca agctcgagga ggtcgcggaa
     5341 gagatccgtt cgtcccgagc gaagtcgagg gaccctatcg agcggagccg agatggttcg
     5401 gcggatgaaa gcctcggctc cgctcggcgg ggtgtctcga cttcgctcga cacgaacggg
     5461 attttcattg cgccggcgga tgtgacgatc cgggaggagg tcgaagccgc cttcgcctcc
     5521 gcccgggagg cgaacggccc gatcaccatc ctcgtcaaca acgcgggcgc ggcagcggga
     5581 gtcgcctttt cgaaagttac cgactcgctc tggcgcgata tgctggcggt gaacctcgac
     5641 ggcatgttcc actgctgcca ggccgccctc cccgacctgc tcgccgccga ggcgggacgc
     5701 atcgttacga tcgcgtcgat ggcaggcctc cacggcttcc cttatgcagc gccctatatc
     5761 gcggccaagc acggcgccgt cggcctcacc cgggcgctgg ccgccgaata tgagcggacg
     5821 ccgctgcgcg tgaacgcggt ctgcccgggc ttcgtcgaca gtgacatgac ggtgcagtcg
     5881 atctccaaca tcaaagccaa gaccggccga agcgacgacg aggcgcgcac cgagctggcc
     5941 aggctcaacc cctccggccg cctcatcaca ccggacgagg tggcggcgga ggcgcttgac
     6001 ctgatccttt cgcagcggaa cggtgaggcg gtggagatag catgaaccct cagaccttcg
     6061 agcccgagca tttcggctgg cgcttcgccg acggcgtcgc cacaatcacc ctcgaccgac
     6121 ccgagcgcaa gaacccactc accttccaaa gctatgccga gctgcgcgac accttccgcg
     6181 acctcgtcta cgaacgcgag gtcagggcgg tggtcgtcac cggcgccggc ggcaatttca
     6241 gctcgggcgg cgacgtccac gagattattg gcccgctgac gggcatggag atgccgggcc
     6301 tgctcgcctt cacccgcatg accggcgatc tcgtcaaggc gatgcgcgcc tgcccgcagc
     6361 cgatcgtcgc cgcgctcgac ggaatctgcg tcggcgccgg cgcgatcctc gccatggcct
     6421 ccgacatccg cctcgccacg cccgcgacca agacggcgtt cctgttcacc cgcgtgggcc
     6481 tcgccggctg cgacatgggc gcgtgcgcga tcctgccgcg catcatcggc cagggcaggg
     6541 cgtccgaatt gctgttcacc ggccgcgtca tgaccgccga ggaaggcgag cgctggggct
     6601 tccacaaccg ccttgtcgcc gtcgacgata tcctcgacga agcccaggac ctcgcccacg
     6661 ccctcgccac cggccccacc ttcgcccacg gcatcaccaa gacccagctc aacaccgaat
     6721 gggcggtgag catcgacgtc gcgatcgaga tggaggcgca ggcccaggcg atctgcatgg
     6781 ccaccaacga cttccgccgc gcctacgaag ccttcgccga caagcagacg ccggagttcc
     6841 agggtgattg agcgcaagcg cttcccgtac gtgcacgggg cgaaagcggc atgagcgatc
     6901 gctccttcct cgactggccg ttcttcgacg acagccaccg tgcgctcgcc gaggcgctcg
     6961 agctttggtg cgaagccgag ctttcggacg aagagccgga ggacgtggac gatgcgtgcc
     7021 gcacgctcgt cgccaagctc ggggccggcg gctggctgcg ctattgcgtg cccgcggcct
     7081 atggcggcgc tcatgaggcg ctcgacgtcc gctcgctggc gctgatccgc gagacgctcg
     7141 cgcgccattc cggcctcgcc gattacgcct tcgccatgca gggcctcggc tcgggcacca
     7201 tcaccctgtt cggcagcgag cagcagaagc aggcctacct ccccgccgtc gcccggggcg
     7261 agaagatcgc cgccttcgcg ctgaccgagc ccggctccgg ctcggacgtc gcggcgatgg
     7321 agaccgccgc ccgcctctcc gagggcggct atgtcgtcga tggcgccaag acctacattt
     7381 cgaacggcgg catcgccgat tattatgtcc tgttcgcgcg caccggcgaa gcccccggcg
     7441 ccaggggcgt ctccgccttt ctggtcgacc gcggcgccga aggcctggac gagagcgagc
     7501 gcatccgcgt catcgcgccg cacccgctcg ccaccctccg cttccacggc atgcgccttc
     7561 ccgccgaagc cctgctcggc accagcgggc agggcttcgc gcaggcgatg gccacgctcg
     7621 acatcttccg caccaccgtc ggcgccgccg ccttgggctt tgcgcggcgc gcgctcgacg
     7681 aggccaccgg ccgcgcgctc gcccgccggc tcggcaatgg cgcgcttgcc gacaatccga
     7741 tgatccaggc gatgctggcg gagatggtgc tcgacgtcga cgcctccgcc ctgctcgtct
     7801 accgcgccgc ctggcttcgg gacgttcggg gcgagcgcaa cagccgcgag gccgcgctcg
     7861 ccaagctcca cgccaccgac agcgcgcaga aggtgatcga caaggcggtc cagattttcg
     7921 gcggcctcgg cgtgacccat ggcgtcccgg tcgagcggct ctaccgcgag atccgcgcgc
     7981 ttcgcatcta tgaaggcgcg tccgaagtgc agaaggtggt gatcgcccgc cgccacctcg
     8041 ccggatattc ggcatgaaga cgctgcttcc ccccggctgg ccgcgcccgc gcggctatgc
     8101 caacggcatc tcggcgcgcg gccgcaccat cttcaccgcc ggcgtggtcg gctgggacga
     8161 ggaggagcgc ttcgccgccc aggacctggt cgggcagttc cgccagatcc tccgcaacac
     8221 cctcgccatc ctcgccgagg gcggcgctgc gcctgagcat atcgtccgca tgacctggta
     8281 cgtcaccgac cgcagcgact atatcgccaa cctccgcgag atcggcgccg tctatcgcga
     8341 gctgatcggc aagcattatc cggcgatggc ggtcgtgatc gtcgcggggc tggtcgagcc
     8401 cgccgccagg atcgagatcg aaaccaccgc gatggtgccc gaatgatcct gccttacgac
     8461 agcttcgtcc gcgaccgcct gccaccgccc gaggcgcagc ccgacttcct gttcgacctt
     8521 cccgagctcg cttatcccga tcggctcaac gccgcggtcg aactgctccg cgccggcccc
     8581 ggcgaggccc cggccgtgat caacgaccat ggcgtgtgga gctatggcga gctgcgcgac
     8641 cgctccgacc gcatcgcccg gctgctggtc gaggaggagg ggctggtccc cggtaaccgc
     8701 gtcttcctgc gcggccccaa tggcgcgatg ctgttcgccg cctggctcgg tattctcaag
     8761 gccggcggca tcgtcgtcgc caccatgccg atcctccgcc ccggcgagat cgccaccatc
     8821 ctcgatcgcg cccaggtcag ccacgccatc gtcgacagcc gcttcatcgg cgacttccgc
     8881 cagggcgccg agcaggcggg cacggtccgc tcgctgatca aatatgatgg cgattacggc
     8941 aagggcgaac tggagtcccg g
//
LOCUS       DASVRK010000190        16071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_534860, whole genome shotgun sequence.
ACCESSION   DASVRK010000190 DASVRK010000000
VERSION     DASVRK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534860"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF589_07440"
     CDS             <1..342
                     /locus_tag="VF589_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX8257450.1"
                     /translation="EFLNRLDEIILFHRLGHEHMGPIVDNQLSRVRKLLADRKVKLEL
                     TDAARAWLGRVGYDPVYGARPLKRAVQKYLQDPLADAILRGEVPDGSTIMIDEGEGAL
                     TLRTGEVRKAA"
     gene            complement(406..1932)
                     /locus_tag="VF589_07445"
     CDS             complement(406..1932)
                     /locus_tag="VF589_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX8257451.1"
                     /translation="MATVPGASAGLNGVARIDPRNADAAFDAGLDALRLGGEKDALRL
                     LAKAARFHPGDARLWQVSGLLHRALDDLDPAVKALTRAAGLARGDARIAHALARARLE
                     AGLPAVDAFEGAHRLAPLDGDVLLGLAAARIADGDSEAAMAGIEAQLRLHPGWLPGHA
                     LIARLRWMFGEREAFTASYERALAAVPRDINLWREMIVTLIHANLYEAALGAIARGRA
                     AAGPHLVFDANEAVCVAELGRTGEADRLFAALGPIGDVTIIIRRIRHLLRSGRPAEAV
                     ALADPLLAGEQAYLIWPYVSVAWRLLGDPRWQWLEGDSRLVGVYDLADQIPSLDALAG
                     RLRALHLAPGQPLEQSVRGGTQTDGPLFARIEPEIRALRKAVVAAVERHVAQLPPPDP
                     RHPTLRMRHGPIRFAGSWSVRLLAAGHHANHIHPMGWFSSALYVTLPSEAEAGPPPAG
                     WLELGRPQAELGVDLPPFRTVEPKRGRLVLFPSTMWHGTMPIAGGERMTVAFDVAPPR
                     "
     gene            complement(1932..3107)
                     /locus_tag="VF589_07450"
     CDS             complement(1932..3107)
                     /locus_tag="VF589_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8257452.1"
                     /translation="MSEPSSTTAQADPREAEADRAAARGDAATAARLLEEITAADPER
                     LEPWLKLAAMRRAQRRIDAALDAVSGALRIQPLGFMPLLLKASLLDAAGRTVEAGEIY
                     GYALAQRPDPVPAHLQRMVAQAEARHAEHLAQAGDRLAAAARTVPLATAEARRLERFR
                     TNILRTTRPYHSEPTHFHYPGLREREYHDRESFPWLARLEQATDAIRADFERVMAAER
                     AELVPYVQYPDDVPVGQWAALNRSRDWTAIHLLRNGSPVEANARHCPTVMELLPHLDQ
                     PHVARRTPNAMFSLLAPGAHIPPHNGIDNSRLVCHLPLIVPEGCWFRVGAETRPWKVG
                     EAWVFDDTIEHEAMNPTDKLRVILIVDTWHPDLSAAERAGVAAVMEATDVDPSAGGM"
     gene            complement(3191..6340)
                     /locus_tag="VF589_07455"
     CDS             complement(3191..6340)
                     /locus_tag="VF589_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8257453.1"
                     /translation="MRKTRFINQLLATTICGAALVAAPAYGQEVQDVNTAEPTTGPVE
                     NTTPDVSAQGEVLEGEGEEAIVVTGSRIPQPNLTAVSPVTVISSQEVKLQGTTRTEDL
                     LNSLPQAFASQGGNLANGATGTATVNLRGLGSARTLVLINGRRLGPGDPTFPAPDINV
                     VPAALIERVDILTGGASSVYGSDAIAGVVNFIMDTDFEGFRIDSQYSFYQHNNNNGTG
                     IIQALEDKGFGYPGGSVADGGTVDVNAVFGTDFADGRGHITAYAGYRKLTEVMQGSRD
                     YSACALQVNAATATNGGENLACGGSGTNAFGTLLTNVGNFEINPAGGLRGAFANPYNF
                     NPTNFYQRPGERYTFGAFAEYEINEAIQPYLEVMFMDDSTVAQIAPSGNFFNTGFINC
                     GAFVEGQTPGTGNAVAGANPTLDAQQAAILCQEANLQGAVADDPDTEENEYQAPSTFV
                     DPFTREEFTRGVAYIGRRNIEGGPRQDNLRHTNYRVAVGSRGELSNAWSYDAYYLFGQ
                     TNFSQAYANELSASRLANALDVITDANGNPICRQAQAGGSAPNCVPYDVFTGTVSPAA
                     LGYLQVTGLAAGEVKQTVASASFTGLLGEYGLQMPWADEGLGINVGVEYRKDQVDYRT
                     DVSFQTGDLTGQGAPSLPVNGAFDVKEIYGEARLPIVTGGFFHYLSLEAGYRYSKYDV
                     ENGTSFSTDSYKIGLDFSPIQDIRFRASYNRAVRAPNVQELFAPQRVALVGNSDPCEG
                     ETPTATQAECALTGLSAAQYGNVPANPASQYSGLIGGNPLLEPEKSTTKSAGVVLRPR
                     FLPGFAATVDYFDIEVANTITTIGVDTIISTCIATGNADFCGRINRDARGTLYLTPAG
                     FTTDLNTNIGGLETRGVDVGASYGTEIGSFGSLNMNFVGTWLDRLVTDNGVSVPYDCA
                     GFFGTQCGTPNPEWRHKARLSWNHPSGVGLSFQWRYFSGVDLDSTSDNETLAGTNTPF
                     NERIGSQSYFDLAGTFRVSDNYAFRVGVNNILDRDPPIIGSNGPTSVCPGVVCSGNTF
                     PQVYDALGRYIFAGVTLDF"
     gene            6591..7121
                     /locus_tag="VF589_07460"
     CDS             6591..7121
                     /locus_tag="VF589_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257454.1"
                     /translation="MKQHALLASLVVVAAPAAVWAAPKLPSQPPAAYSALLRCRGMTD
                     QAARLACFDSAVAALDTATRNRELVVVDREQIRRTRRSLFGLDLPNFRLFGDDAEEAD
                     EVKTIEGVVASVGSDANARYIIRLKDGSTWRQMDGNTLGRGPRAGYKVVINRTMTGSY
                     MMRVEGQPGIRVKRTV"
     gene            complement(7144..8022)
                     /locus_tag="VF589_07465"
     CDS             complement(7144..8022)
                     /locus_tag="VF589_07465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8257455.1"
                     /translation="MKPGALALALVAACAAAVAAAAATAADAGQAVASPAALEAPRFR
                     FEGNMRQGGVVLGSAPAGTVEITLDGKPVRVGADRRFILGFDRDQGPEAILAARLADG
                     RAVTETLRIAPGSWRIEHIGIARRPTQSTEEYLRLRERELARIGAARSRAPPSEGWRQ
                     RFAWPARGRISGLFGSQRIYRGEPGAYHSGVDVAAGAGAAVVAPADGIVVLAGPPAFS
                     LEGNLVIIDHGMGLNSAFLHLATQAVRQGQAVRQGQLIGTVGASGRATGPHLHWSMKW
                     NEARIDPQALAGPMRP"
     gene            complement(8019..8219)
                     /locus_tag="VF589_07470"
     CDS             complement(8019..8219)
                     /locus_tag="VF589_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEX8257456.1"
                     /translation="MLMANQGRPAKLHYMAGTFRVLSSGDHVICAVTGQRIPLEELRY
                     WSVARQEAYADAAAAVRAESNR"
     gene            complement(8233..9678)
                     /gene="xseA"
                     /locus_tag="VF589_07475"
     CDS             complement(8233..9678)
                     /gene="xseA"
                     /locus_tag="VF589_07475"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX8257457.1"
                     /translation="MNDFPSTDAGLLATEMPGDNAPAQTVSELAMRLKRTVEDAYGLV
                     RVRGEISGWKRAASGHCYLCLKDDKAVIDGVMWKATAGALRFRPEDGAEVIATGKLTT
                     YPGRSRYQIVIDRLELAGQGALMALLDKRRRALAAEGLFDDARKRRLPFLPRTIGVVT
                     SPTGAVIRDILHRLADRCPSHVVVWPVPVQGEGAAAQIAAAIRGFDAIAPGGPVARPD
                     LVIVARGGGSIEDLWAFNEEEVVRAVADCSIPIISAVGHETDTSLCDFAADVRAPTPT
                     AAAEIAVPVRAELLAQLRELGGRTARCAARAFERGGEGLANCLRHFPAPEALLAPQRQ
                     RLDDLAERLPRGLGGRLSHARADLGHAAGALRPSLLGAALRRGRERLGAVRLDAKLVE
                     RRIADGRRSLDGLWRIAALAHPNKPLEKGYVRVEDRDGRTLISAARARAAGRLRLVFG
                     DGKLDVAVGERVERPRAAPYAPPKSEQPKLL"
     gene            9677..10960
                     /gene="purD"
                     /locus_tag="VF589_07480"
     CDS             9677..10960
                     /gene="purD"
                     /locus_tag="VF589_07480"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX8257458.1"
                     /translation="MNVLLLGSGGREHALAWKLAQSPKLGKLFAAPGNPGIAEHAQLV
                     AVDPADHRGTMDFCLKNSVELIVIGPEAPLVDGLADNLRTMGFCVFGPNRVPAQLEGS
                     KGFTKDLCARRRIPTARYARVSDPLAAEAALGDFALPVVIKADGLAAGKGVVIAATTA
                     EAEAAIRDMLAGGFGAAGASLVIEEFLAGEEASFFALTDGETVVPFGSAQDHKRVGDG
                     DTGPNTGGMGAYSPASILTPEIEERVMDEIVCPTVEAMRANGTPYSGVLYAGLMLTAE
                     GPKLIEYNVRFGDPECQVLMMRLESDLLALMCAVAKGKLAEAEPPRFSDEAALTIVMA
                     ANGYPGTPEKGGAISGIEAAEAGGARIFQAGTALDGGGLVASGGRVLNVTARGATVAE
                     AQQTAYAAAAAVDFSSGFYRRDIGWREVERLGSGA"
     gene            11028..11459
                     /locus_tag="VF589_07485"
     CDS             11028..11459
                     /locus_tag="VF589_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257459.1"
                     /translation="MSRPVKFLIGLAAVILMGWIYHGPLGNGEAFVSRLEGQARSAVA
                     ATGLPGIEVRLSRDPLSRAATLSGPADEFQREGQGSLPGLNDLVREVEGISDVGWADH
                     DPADPGLPVLAELLIGIVAAYLLGVGLASLLFGRAKKESYL"
     gene            11482..12042
                     /locus_tag="VF589_07490"
     CDS             11482..12042
                     /locus_tag="VF589_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257460.1"
                     /translation="MTTVFENNLVPIIVALLIGLLIGWWMFRSVRRGRPGDRPGRDTI
                     EATPPAMRAPAPPVRPYVDGRRQDGPEGNNIADHGAAAAADVAGQVLGVSAHSQLPGA
                     SGPPDNLQMLKGVGPKLAEKLNDNGIIRFEQLARLSTNEVDMLESRLGPFKGRLARDR
                     VVEQASYLARDDRDGFEARFGKLGGP"
     gene            complement(12196..13509)
                     /locus_tag="VF589_07495"
     CDS             complement(12196..13509)
                     /locus_tag="VF589_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX8257461.1"
                     /translation="MSPAPPPLTPFPWFDVLVIALLIGLNGVFAMSELAIVSAREARL
                     KGMAKAGRRGAEAALKLAADPGRFLSTVQIGITLIGILAGAYSGASLGGPVGQRLARL
                     GIAPDDAQTLGFAVVILLTTYASLIIGELVPKQFALRGAEQIAVFMAPPMQWLAKATA
                     PLVWMLDRTSALIFRLLGLKRETENMVTAEELHLVVAEAHSAGVLEESERAIISGVVR
                     LADRPTREVMTPRTEVEWIDIDADPEAIRRKLAETPHNRLPVAQGSVDRLVGVVQSRD
                     IVKAFVAGLPLDLRALMRTAPVVPDQMDAMDALLVLRSADVPMAFVHDEYGHFDGLVT
                     PADLLAALAGGFNSDADEDPPLVEREDGSWLVSGSLSADALADTLGINLPEDRDYATA
                     AGFALWVLKHLPETGERFAFGGWNFEVVDMDGRKIDKLIASQASG"
     gene            complement(13709..14137)
                     /locus_tag="VF589_07500"
     CDS             complement(13709..14137)
                     /locus_tag="VF589_07500"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX8257462.1"
                     /translation="MVLALDAARAAAAAGEVPVGAVVARGAEILAVAGNAMRGAHDPT
                     AHAEMAALREAARRLGSSRLDDCDLWVTLEPCAMCAGAIALARIRRLYFAAPDPKGGA
                     VVHGPRLFAQPTCHHAPEVYPGLGEQAATALLRDFFRERR"
     gene            complement(14265..14537)
                     /locus_tag="VF589_07505"
     CDS             complement(14265..14537)
                     /locus_tag="VF589_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257463.1"
                     /translation="MSRKVLGILVVVVLIVIALFASGFWKADVKEGALPDVDVTAEGG
                     AMPGVDVDSKEVVVGTTETNISVPKIETEETKVDVPVIGVKDNGEE"
     gene            14727..15023
                     /gene="rpmB"
                     /locus_tag="VF589_07510"
     CDS             14727..15023
                     /gene="rpmB"
                     /locus_tag="VF589_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX8257464.1"
                     /translation="MSRICELTGKGRLVGHNVSHANNKTKRTFLPNLQNVTLMSDALQ
                     RGVKLRVSMSGLRSIEHVGGLDNWLAKTSDEKLSLKARRLKREIRKKDADAASA"
     gene            complement(15033..15992)
                     /locus_tag="VF589_07515"
     CDS             complement(15033..15992)
                     /locus_tag="VF589_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX8257465.1"
                     /translation="MRLLAALVAFPLVTTFAPPALFRDAPLPALIAIGFDPVPLDTEH
                     LARRRLGRLVYLGGWSIRSNDPRFGGISAMHVEDGRVLALSDSGSLIEFGLPGRPGPV
                     SIRPLPDGPRGDRGKLDRDAETLAVSGDKAWIAFEGRNAIWRYSRPSWRAESDAEPAP
                     MRRWPGNTGSEALLRFPDGRFAVFAEHWLRPNGSSDVVAFAGDAAVPGTPTKTLGYRP
                     PKNYRVTDAALLADGRILFLNRRFSLLHGISAKLTVAAPRLAAGSVIEGEEIAHFDAP
                     VTVDNMEALSVTREAGRTIVWMASDDNFNALQRTLLLKFELTE"
BASE COUNT         2528 a         5387 c         5724 g         2432 t
ORIGIN      
        1 gagttcctca accggctcga cgagatcatc ctgttccacc ggctcggcca tgagcatatg
       61 ggcccgatcg tcgacaacca gctcagccgg gtccggaagc tgctcgccga ccgcaaggtg
      121 aagctggagc tgaccgacgc cgcgcgcgcg tggctcgggc gcgtcggcta cgatcccgtc
      181 tacggagcgc ggccgctgaa gcgggcggtg cagaaatatc tgcaggatcc gctggccgac
      241 gcgatcctgc gcggcgaggt gccggacggc tcgacgatca tgatcgacga aggcgaaggc
      301 gcgctgactt tgcggaccgg cgaggtgcgc aaggcggcgt aaggtccggg tggtcattcc
      361 agcttccgct gggatgacgc atagccgctc gggcgacggc cgccgtcagc gcggcggggc
      421 gacgtcgaag gcgacggtca tccgctcgcc gccggcgatc ggcatggtgc cgtgccacat
      481 ggtcgagggg aacaggacca gccgcccccg cttcggctcg accgtacgga acggcggcag
      541 gtcgacgccg agctcagcct gcggccggcc cagctccagc cagccggccg gcggcgggcc
      601 cgcttcggcc tcggacggca gcgtcacata gagggccgag ctgaaccagc ccatcgggtg
      661 gatgtggttg gcatggtggc cggcggcgag cagccgcacg gaccaggagc cggcgaagcg
      721 aatcggcccg tgccgcatcc gcagcgtcgg gtggcgcggg tcgggcggcg gcaattgcgc
      781 gacgtgccgc tcgaccgccg ccaccaccgc cttgcggagc gcccggattt cgggctcgat
      841 ccgcgcgaac agcggcccgt cggtctgggt gccgccgcgg accgactgtt cgagcggctg
      901 gccgggcgcg aggtgaagcg cgcgcagccg accggcgagc gcgtccagcg acgggatctg
      961 gtcggcaaga tcgtagacgc cgaccagccg cgaatcgcct tcgagccatt gccagcgcgg
     1021 gtcgcccagc aggcgccacg cgaccgagac atagggccag atgagatagg cctgctcgcc
     1081 ggcaaggagc gggtcggcga gcgcgaccgc ctcggccggg cgtccggaac ggagcaggtg
     1141 gcggatgcgg cggatgatga ttgtgacgtc gccgatcggg cccaaggctg cgaacagccg
     1201 gtcggcctcg ccggtgcgcc cgagctcggc cacgcagacc gcctcgttgg cgtcgaacac
     1261 caggtgcggg cccgcggccg cgcgaccgcg tgcgattgcg cccagcgccg cttcgtagag
     1321 gttggcgtgg atcagggtaa cgatcatttc gcgccacagg ttgatgtcgc gcggcaccgc
     1381 ggcgagagcg cgctcgtagg aggcggtgaa agcctcccgc tcgccgaaca tccagcgcag
     1441 gcgggcgatc agcgcgtgcc cggggagcca gccggggtgc agccgcaatt gcgcctcgat
     1501 acccgccatc gccgcttccg agtcgccatc cgcgatccgc gcggcggcga ggccgagcag
     1561 gacgtcgccg tcgagcggcg ccagccggtg cgccccctcg aacgcgtcca cggcgggcaa
     1621 gccggcctcg agcctggcgc gggcgagggc atgggcgatc ctggcgtcgc cgcgcgccag
     1681 cccggccgcc cgggtcagcg cctttaccgc cggatccagg tcgtcgagcg cccggtggag
     1741 gagcccgctc acctgccaga gccgcgcgtc gccgggatgg aagcgggccg ccttcgcgag
     1801 caggcggagc gcgtccttct cgccgccgag cctcagcgca tcgagccccg catcgaaggc
     1861 cgcatcggca ttgcgtggat cgattcgcgc cacgccattg aggccggcgc tggcgccggg
     1921 aacggtggcc atcacattcc tccggcggac ggatccacgt cggtcgcttc catcacggcg
     1981 gcgacgcctg cgcgctcggc ggccgacaga tcgggatgcc aggtgtcgac gatcaggatc
     2041 acccgcagct tgtcggtcgg gttcatcgcc tcatgctcaa tcgtatcgtc gaacacccaa
     2101 gcctcgccga ccttccacgg gcgggtctcg gcgccgacgc ggaaccagca gccctccggg
     2161 acgatcagcg gcaggtggca gaccagccgg ctgttatcga tgccgttatg cggcgggatg
     2221 tgcgcgccgg gcgccagcag cgagaacatc gcgttaggcg tgcgccgggc gacatgaggc
     2281 tggtcgagat ggggcagcag ctccattacg gttgggcagt ggcgggcatt ggcctcgacc
     2341 gggctgccgt tgcgcaggag atggattgcg gtccagtcgc gcgaccggtt gagcgccgcc
     2401 cattgcccga ccgggacgtc gtcgggatat tggacatagg gcacgagctc cgcccgctcg
     2461 gcggccatga cccgctcgaa atcggcgcgg atcgcatcag tcgcctgttc gagcctcgcc
     2521 agccacggaa agctctcgcg gtcgtgatat tcgcgttcgc gcaggccggg ataatgaaaa
     2581 tgggtcggct cggaatgata ggggcgggtg gtgcgcagga tgttcgtccg gaaccgctcc
     2641 agccggcgtg cctcggcggt cgcgagcggc accgtccgcg ccgccgccgc cagccggtcg
     2701 ccggcctgcg cgagatgctc ggcatgacgc gcctccgcct gcgcgaccat gcgctgcaag
     2761 tgcgcgggta ccggatcggg ccgctgtgcg agcgcatagc cgtagatttc gcccgcctcg
     2821 acggtgcggc cggcggcgtc gagcaagctc gccttgagga gcagcggcat gaagccgagc
     2881 ggctggatcc tgagcgcgcc cgagaccgca tcgagcgccg cgtcgatccg gcgttgcgcg
     2941 cgccgcatcg ccgccagctt cagccacggc tcgaggcgct cggggtcggc cgccgtgatc
     3001 tcctcgagca gccgggccgc ggtcgcggca tcgccgcggg cggcggcacg gtcggcctca
     3061 gcctcgcgcg gatcggcctg tgcggtggtt gaactcggct ccgacatgag tcgcttctag
     3121 cgggcgcggc gcaaaagaaa acggcggacc cgaaggcccg ccgtcaagtt tggtgctgat
     3181 gtcgttcgac ttagaagtcg agggtgacac cggcgaagat gtagcggccg agggcgtcat
     3241 agacctgcgg gaaggtgttg ccggagcaaa ccacacccgg gcagacgctg gtcgggccgt
     3301 tcgagccgat gatcggggga tcacggtcga ggatgttgtt gacgccgacg cggaaggcat
     3361 agttgtcgct gacgcggaac gtgcctgcca gatcgaagta gctctgcgaa ccgatccgct
     3421 cgttgaacgg cgtgttcgtg cccgcaagcg tttcattgtc gctggtcgag tcgaggtcca
     3481 cgcccgagaa gtagcgccac tggaaggaga ggccgacgcc gctcgggtgg ttccagctaa
     3541 gccgggcctt gtgacgccat tcggggttcg gagtgccgca ctgggtaccg aagaagccgg
     3601 cgcagtcata aggcaccgaa accccgttgt cggtgaccag gcggtcaagc caggtgccga
     3661 cgaagttcat gttcagcgaa ccgaacgaac cgatctccgt gccgtagctc gcgccaacgt
     3721 cgacgccccg cgtttcgagg ccaccgatgt tggtgttgag gtcggtcgtg aagccggccg
     3781 gggtcaggta cagcgtgccg cgcgcgtcac gattgatccg gccgcagaag tcggcattgc
     3841 cggtcgcgat gcaggtgctg atgatcgtat cgacgccgat cgtggtgatg gtgttcgcca
     3901 cctcgatgtc gaaataatcg accgtcgcgg caaagcccgg caggaagcgc ggccgaagca
     3961 cgacgccagc ggacttcgtg gtcgactttt ccggctccag gagcgggttg ccgccgatca
     4021 ggccgctata ctgcgacgcc gggttggccg gaacattgcc gtactgagcc gccgagaggc
     4081 cggtgagggc gcactcggcc tgggttgccg tcggagtctc gccttcgcaa ggatcggagt
     4141 tgccgaccag ggcgacacgc tgcggagcga acagctcctg gacgttcggc gcgcggacgg
     4201 cgcggttgta gctggcgcgg aagcggatgt cctggatcgg cgagaaatcg aggccgatct
     4261 tgtaggagtc cgtcgagaag ctggtgccgt tttcgacgtc gtatttcgaa taacggtagc
     4321 cggcttcgag cgacagatag tggaagaaac cacccgtgac gatcgggagg cgggcctcgc
     4381 cgtagatctc cttgacgtcg aacgcaccgt tgaccggcag cgacggagcg ccctggccgg
     4441 tcagatcgcc ggtctggaac gacacgtcgg tacgatagtc gacctggtcc ttgcgatact
     4501 cgacgccgac gttgatgccg aggccttcgt ccgcccaggg catctggagg ccgtattcgc
     4561 cgagcaggcc ggtgaaggag gctgaagcga cggtctgctt cacttcgccg gcggcgaggc
     4621 cggtgacctg caggtagccg agagccgccg gcgacacggt gccggtgaac acgtcgtagg
     4681 ggacgcagtt cggagcgctg ccaccggcct gcgcctgacg gcagatcggg ttgccgttgg
     4741 cgtcggtgat gacgtcgagg gcgttggcga ggcgcgaggc cgagagctcg ttggcataag
     4801 cctgcgagaa gttggtctgg ccgaacaggt agtaagcgtc atacgaccag gcgttgctga
     4861 gctcgccgcg cgagccgacc gcgacgcggt agttggtgtg gcgcaggttg tcctgacgcg
     4921 gcccgccttc gatgttacga cggccgatat aggcgacgcc gcgggtgaac tcttcgcgcg
     4981 tgaaaggatc gacgaaggtc gacggagcct gatactcatt ctcttccgtg tcgggatcgt
     5041 cagccacggc gccctgaagg ttggcctcct ggcagaggat ggcggcctgc tgcgcatcga
     5101 gcgtcgggtt ggcgccggcg acggcgttgc cggtgccggg ggtctgaccc tcgacgaagg
     5161 cgccgcagtt gatgaagccg gtgttgaaga agttacccga cggggcgatc tgggcgaccg
     5221 tggagtcgtc catgaacatg acttcaagat aaggctggat ggcctcgttg atttcatatt
     5281 cggcgaacgc gccgaacgtg taacgctcgc cgggccgctg gtagaagttg gtcgggttga
     5341 agttgtaggg attggcgaag gcgccacgga ggccgccggc cggattgatt tcgaagttgc
     5401 cgacgttggt cagcagggtg ccgaaggcgt tggtgcccga gccgccgcaa gccagattct
     5461 cgccgccgtt ggtggccgtc gcggcattca cctgaagggc gcaggcgctg tagtcgcggc
     5521 tgccctgcat gacttcggtc agcttacgat agccggcata agcggtgatg tggccgcggc
     5581 cgtcggcgaa gtcggtgccg aacacggcat tgacgtcgac cgtgccgcca tcggcgacgc
     5641 tgccgccggg atagccgaag cccttgtcct cgagcgcctg gatgatgccc gtgccgttgt
     5701 tgttgttgtg ctgatagaag ctgtactggc tgtcgatgcg gaagccttcg aagtcagtat
     5761 ccatgatgaa gttcacgacg cccgcgatgg cgtccgaacc gtaaaccgac gaggcgccgc
     5821 cggtgaggat gtcgacgcgc tcgatgaggg cggccggaac gacgttgatg tccggagccg
     5881 ggaaggtggg atcgcccggg ccgaggcgcc ggccgttgat cagcaccagg gtacgggccg
     5941 aaccgaggcc gcgcaggttg acggtggcgg taccggtcgc gccgttggcg aggttgccgc
     6001 cctgcgaggc gaacgcctgc ggcaggctgt tcagaaggtc ttcggtgcgg gtcgtgccct
     6061 gcagcttgac ttcctggctg ctgatgacgg tgaccgggct caccgcggtg aggttcggct
     6121 gcggaatgcg cgaaccggta acgacgatcg cttcctcgcc ctcgccttcg aggacctcac
     6181 cctgcgccga cacgtcggga gtggtgttct caaccggacc ggtggtcggc tcggcggtgt
     6241 tcacgtcctg cacttcctgg ccgtaggcag gagctgccac aagcgccgca ccacagatgg
     6301 tcgtggccag caattggttg ataaagcgag tctttctcat ttcgttcgtc cccttaagcg
     6361 gtctggcgcc gctgcgtcac aaatcctgga acgccggcat agacgccgac aattcagcct
     6421 ccatctacgg gatgtaatca aaaggacgca atcgtcccgc caagggaaag cggccatttt
     6481 tcagcctcgt gataaatttg ccacataggt ctgtggctcc ggaccggcac gttgccatgg
     6541 ccgatctcat ccttcatatc tcggcgcctc aacctaattc gcgggacaat atgaaacagc
     6601 acgccctcct cgcctcgctc gtcgtcgtcg ccgcgccggc cgcggtctgg gccgcgccga
     6661 agctcccctc gcagccgccg gccgcctatt ccgcgctgct gcgctgccgg gggatgaccg
     6721 accaggcggc ccgcctcgcc tgcttcgaca gcgccgtggc cgcgctcgac accgcgaccc
     6781 gcaatcgcga gctcgtcgtc gtcgatcgcg agcagatccg caggacgcgg cgctcgctgt
     6841 tcgggctcga cctgccgaac ttccggctgt ttggagacga cgcggaggaa gccgacgagg
     6901 tcaagaccat cgaaggggtg gtcgcctcgg tgggatcgga cgccaacgcc cgctacatca
     6961 tccgcctgaa ggacggttcg acgtggcggc agatggacgg caacacgctt ggccgcggcc
     7021 cgcgtgccgg ctacaaggtg gtgatcaacc gcaccatgac cggcagctac atgatgcggg
     7081 tcgaggggca gccggggatc cgggtcaagc gaaccgtctg agccgctact tccagcgccc
     7141 gtttcaaggc cgcatcggcc ccgccagcgc ctgcgggtcg attcgggcct cgttccactt
     7201 catgctccag tggaggtgcg gcccggtcgc gcgtccgctc gcgccgacgg tgccgataag
     7261 ctggccctgc cggaccgcct ggccctgccg caccgcctgg gtggcgaggt ggaggaaggc
     7321 gctgttgagc cccatgccgt gatcgatgat gacgaggttg ccttcgaggc tgaacgccgg
     7381 cggccccgcg agcaccacga ttccgtcggc gggcgccacc accgcggccc ccgcgccggc
     7441 cgccacgtct actccggaat gataggcgcc cggctcgccg cgatagatgc gctgcgagcc
     7501 gaacaggccg ctgatccgcc cccgcgccgg ccaggcgaat cgctgccgcc agccctcgct
     7561 cggcggcgcc cggctgcgag cggcaccgat tcgcgcaagc tcccgctccc gcaggcggag
     7621 atattcctcg gtcgattgag tcgggcggcg ggcgatgccg atatgctcga tcctccagct
     7681 gccgggcgcg atccgcagcg tctcggtgac ggcgcggccg tcggccagcc gggcggcgag
     7741 aattgcctca ggcccctggt cgcggtcgaa gccgaggatg aagcgccggt cggcgccgac
     7801 tcgcaccggc ttgccgtcga gcgtgatctc gaccgtaccg gcgggcgcgc tgcccaacac
     7861 caccccgccc tggcgcatat tcccctcgaa gcggaagcgc ggcgcctcca gcgcggcggg
     7921 cgacgcgacg gcctgccctg catcggcagc ggttgcggct gcggctgcca ccgccgccgc
     7981 gcaggcggcg accaaggcaa gcgccagcgc gcccggtttc atcggttcga ttcggccctg
     8041 accgcggccg cggcgtccgc ataagcctcc tgccgcgcga cgctccaata gcgcagctcc
     8101 tcgagcggaa tgcgctggcc ggtgacggcg cagatcacgt gatcgccgga gctgagcacg
     8161 cggaaggtgc cggccatata gtggagcttg gcgggcctgc cctgattcgc catcagcatg
     8221 ttagttcgtc cgtcacagca gcttgggttg ttccgatttc ggcggcgcat agggggccgc
     8281 gcgcgggcgc tcaaccctct cgccgacagc gacgtcgagc ttgccgtcgc cgaagacgag
     8341 gcggagccgg ccggccgccc gcgcccgcgc cgccgagatc agcgtgcgcc cgtcgcggtc
     8401 ctcgacccgc acatagccct tttcgagcgg cttgttcgga tgggcgagcg cggcgatccg
     8461 ccacagcccg tcgagcgagc gccgcccgtc ggcgatccgg cgctcgacca gcttggcatc
     8521 gagccggacc gcgccgagcc gctcgcggcc gcggcggagg gcggcgccga gcagcgacgg
     8581 gcgaagcgcg ccggcggcgt ggccgagatc ggcgcgggca tggctgaggc ggcctcccag
     8641 cccgcgcggc agccgctcgg cgagatcgtc gagccgctgc cgctgcggag cgagtagcgc
     8701 ctcgggcgcc gggaaatggc gcaggcaatt tgcgagcccc tcgccgccgc gttcgaacgc
     8761 gcgggccgcg caacgggcgg tgcggccgcc gagctcgcgc agctgggcga ggagctcggc
     8821 gcggaccggg accgcgatct cggccgccgc ggtcggggtc ggcgcgcgca cgtcggcggc
     8881 gaaatcgcac agcgacgtgt ccgtctcgtg gccgaccgcc gagatgatcg ggatcgagca
     8941 gtcggcgacg gcgcgcacca cctcctcctc gttgaacgcc cacagatcct cgatcgagcc
     9001 gccgccgcgg gcgacgatga cgaggtcggg ccgcgcgacc gggccgccgg gggcgatcgc
     9061 gtcgaagccg cgaatcgcgg cggcgatctg ggcggccgcc ccttcgccct gcaccggcac
     9121 cggccatacg acaacgtggc tcgggcaacg gtcggcaagg cggtggagga tgtcgcgaat
     9181 caccgcgccg gtcggtgagg tcacgacacc gatggtgcgc ggcaggaacg gcaggcgccg
     9241 cttgcgggcg tcgtcgaaca gcccttcggc ggcgagcgcc cggcggcgct tgtcgagcaa
     9301 cgccatcaaa gcgccctgtc cggcgagctc gaggcggtcg atgacgatct ggtagcgcga
     9361 acggccggga taggtagtga gcttgccggt cgcgatcact tcggcgccat cctcgggcct
     9421 gaagcggagc gcgccggccg tcgccttcca catgacgccg tcgatcaccg ccttgtcgtc
     9481 ctttaggcag aggtagcaat ggccggacgc ggcgcgcttc cagccggaaa tctcgccgcg
     9541 cacgcggacc agcccgtaag cgtcctcgac cgtgcgcttg agccgcatgg ccagttcgga
     9601 gacggtctgg gcgggcgcgt tgtcgccggg catttcggtt gctaacagcc ccgcgtcagt
     9661 ggaagggaaa tcgttcatga atgtcctgtt gctcggctcg ggcgggcgcg aacatgcgct
     9721 cgcgtggaag ctcgcgcaat cgccgaagct cggtaagctg ttcgccgcgc ccggcaatcc
     9781 cggcatcgcc gaacatgcgc agctggtcgc ggtggacccc gccgaccatc gcggcacgat
     9841 ggacttctgc ctcaagaatt cggtcgagct gatcgtgatc ggccccgaag cgccgctcgt
     9901 cgacggcctt gccgacaatc tccgcacgat gggcttttgc gtgttcgggc ccaaccgggt
     9961 gccggcccag ctcgaaggct ccaagggctt caccaaggat ttgtgcgccc gccgccgcat
    10021 cccgaccgcg cgctatgccc gcgtgtcgga cccgctcgcg gccgaggccg cgctcggcga
    10081 tttcgccctg ccggtggtga tcaaggccga cgggctggcg gcgggcaagg gcgtggtgat
    10141 cgccgccacg acggccgagg ccgaggcggc gattcgcgac atgctcgccg gcggcttcgg
    10201 cgcggccggc gcgagcctgg tgatcgagga atttctcgcc ggggaggaag ccagcttctt
    10261 cgcgctcacc gacggcgaga cggtggtccc gttcggctcc gcgcaagacc ataagcgggt
    10321 cggcgacggc gacaccggac ccaataccgg cggcatgggc gcctacagcc cggcctcaat
    10381 cctgaccccc gaaatcgaag agcgggtgat ggatgagatc gtctgcccga ccgtcgaggc
    10441 gatgcgggcc aacggcactc cgtattcggg cgtactctat gccgggctga tgctgaccgc
    10501 ggaggggccg aagctgatcg aatataatgt gcggttcggc gaccccgaat gccaggtgct
    10561 gatgatgcgg ctggaaagcg acctgctggc gctaatgtgc gcggtcgcca agggcaagct
    10621 cgccgaggcg gagccgccgc gtttctcgga cgaggccgcg ctcaccatcg tgatggccgc
    10681 caatggctat ccgggcacgc ccgaaaaagg cggcgccata tcggggatcg aagcggccga
    10741 ggccggcggc gccaggatat tccaggccgg gaccgcgctc gacggcggcg ggctggttgc
    10801 cagcggcggc cgcgtgctca acgtcaccgc gcgcggcgcg acggtggcgg aagcgcagca
    10861 gaccgcttac gccgccgccg ccgcggtcga tttcagctcc ggcttctacc gccgtgacat
    10921 cggctggcgc gaggtcgagc ggctcgggtc cggcgcttga tcgcggccgc ccttcggagg
    10981 atactttccg gaaccgaaac attgaaccac ggcgaagggg aaagtcgatg tcgcgcccag
    11041 tcaagttcct gatcggcctc gccgccgtga tcctgatggg ctggatctat cacggcccgc
    11101 tcggcaatgg cgaagccttc gtcagccggc tcgaaggcca ggcgcgaagc gccgtcgcgg
    11161 cgaccgggct gccgggaatc gaggtgcgcc tcagccgcga tccgctctcc cgggccgcga
    11221 ctctgtccgg cccggcggac gagttccagc gcgaagggca agggtcgctc cccggcctca
    11281 atgatctcgt ccgcgaggtg gaggggattt cggacgtcgg ttgggccgat cacgaccccg
    11341 ccgaccccgg cctgcccgtg cttgccgagc tgctgatcgg gatcgtggcg gcctatctgc
    11401 tgggcgtcgg cctcgcctcg ctgctgttcg ggcgggcgaa gaaggaaagc tatctctgag
    11461 gcgtctcacc aaggaacgtt catgacgaca gtgttcgaga acaatctggt ccccatcatc
    11521 gtcgcattgc tgatcgggct gctgatcggc tggtggatgt tccggagcgt ccggcggggc
    11581 cgccccgggg accggccggg gcgcgacacg atcgaggcga cgccgcccgc gatgcgcgcg
    11641 ccggccccgc cggtgcgtcc ctatgtcgac gggcggcggc aggacggccc cgagggcaac
    11701 aatatcgcgg accatggcgc cgcggccgcc gccgacgtcg ctggccaagt gctgggcgtc
    11761 agcgcccatt cccagcttcc cggcgccagc ggcccgcccg acaatctgca gatgctgaag
    11821 ggggtcggcc cgaagctcgc cgaaaagctc aacgacaacg gcatcatccg cttcgagcag
    11881 ctggcgcggc tgagcacgaa cgaggtcgac atgctcgaat cgcggctcgg cccgttcaag
    11941 ggccggctgg cccgcgaccg cgtcgtcgag caggccagct acctcgcccg cgacgaccgc
    12001 gacgggttcg aggcgaggtt cggcaagctc ggcgggcctt agcgctcgct ccggtaacct
    12061 tgcatcatgc cggacttgat ccggcatccc gctttcttcc tcgcggagcc aagccccgaa
    12121 ggagcgggac cccggatcaa gtccggggtg acgattcacc caagtccggg gtgacgattc
    12181 acgcggggcg tgcgttcagc cggacgcctg gctggcgatc agcttgtcga tcttgcggcc
    12241 gtccatgtcg accacctcga aattccagcc gccgaaggcg aagcgctccc cggtctcggg
    12301 caaatgcttc agcacccata gggcgaagcc ggcggcggtg gcgtaatcgc ggtcctcggg
    12361 cagattgatg ccgagcgtgt cggccagcgc atcggcggag agcgagccgg acaccagcca
    12421 gctgccatcc tcgcgctcga ccagcggcgg atcctcgtcc gcatcggagt tgaaccctcc
    12481 ggcgagggcg gcaagcaggt cggccggggt taccagcccg tcgaaatggc cgtattcgtc
    12541 gtggacgaag gccatcggca cgtcggccga gcgaagcacc aacaaagcgt ccatcgcgtc
    12601 catctggtcc ggcaccaccg gcgcggtccg catcagcgcg cgcagatcga gcgggaggcc
    12661 ggcgacgaac gccttgacga tgtcgcgcga ctggacgacc ccgaccagcc ggtcgaccga
    12721 cccctgcgcc accggcagcc ggttgtgcgg cgtctcggcg agcttgcggc gaatcgcctc
    12781 cggatccgcg tcgatgtcga tccactcgac ctcggtgcgg ggcgtcatca cctcgcgggt
    12841 cgggcggtcg gcgaggcgga ccacgcccga gatgatcgcc cgctcgctct cctcgagcac
    12901 gcccgcgctg tgcgcttcgg cgacgaccag gtgcagctct tcggcggtca ccatattctc
    12961 ggtctcgcgc ttcaggccca gcaaccggaa aatgagcgcg ctggtgcgat cgagcatcca
    13021 caccagcggc gcggtcgcct tggccagcca ctgcatcggc ggggccatga acacggcgat
    13081 ctgttcggcg ccgcggagcg cgaactgctt gggcaccagc tcgccgatga tcagcgaggc
    13141 ataagtagtg aggaggatga cgacggcgaa gccgagcgtc tgggcgtcgt ccggggcaat
    13201 ccctagccgc gccagccttt gcccgaccgg gccgccgagg ctcgcgcccg aataggcgcc
    13261 ggcgaggatg ccgatgaggg tgatgccgat ctgcacggtc gacaggaacc gccccggatc
    13321 ggcggccagc ttcagcgccg cctcggcgcc gcggcgcccg gccttggcca tgcccttgag
    13381 ccgcgcctcg cgcgccgaga cgatggcgag ctccgacatg gcgaacacgc cgttcagccc
    13441 gatcagaagg gcgatcacga gcacgtcgaa ccaggggaaa ggcgtcaggg gcgggggggc
    13501 gggggacatg gactgccgct cccccttagc cgcgaacctc ggcgcgcgcc aacatcttca
    13561 tggggagggg attggagggg ggttcgtgga cgagaggcct tggccgatca gctcgtcact
    13621 ccggacttga tcgggggtcc ctcttcttct gctcgtcggg gtggaagggt gaagggagat
    13681 gccggatcaa gtccggcatg acgagtgctc agcggcgctc cctgaagaag tcccgcagca
    13741 gcgcggtcgc cgcttgctcg cccaggcccg gatagacttc gggcgcatgg tggcaggtgg
    13801 gctgggcgaa cagcctcggc ccgtggacga cggcgccgcc cttggggtcg ggcgcggcga
    13861 aatagaggcg gcggatgcgg gcgagggcga tcgcgccggc gcacatcgca cagggctcga
    13921 gcgtcaccca caggtcgcaa tcgtcgagcc gggaactccc gagccgccga gccgcttccc
    13981 gcagcgccgc catttcggcg tgggcggtgg gatcgtgcgc gccgcgcatc gcattgccgg
    14041 ccaccgcgag gatttcagct ccacgcgcga cgacggcgcc gaccggcacc tcgcccgccg
    14101 ccgccgcggc gcgggcagcg tcgagcgcaa gcaccatcgg ttcgggaagc ggaaacggca
    14161 ccgccgccga atagcggctg atcccgtcaa tgaccatgtc gccgggaaag gcgggtcatc
    14221 ggccgcggcg gggccaagcc tcgccgccgc ggccgcccct tggcttattc ctcgccgtta
    14281 tccttgacgc cgatgacggg gacgtcgacc ttcgtctctt cggtctcgat cttgggaacc
    14341 gagatgttgg tttcagtggt gccgacgacg acctccttcg aatcgacgtc gactccaggc
    14401 atggcgccgc cttcggcggt gacgtcgaca tcgggaagcg cgccttcctt gacatcggcc
    14461 ttccagaagc cgctcgcgaa cagagcgatg acgatcagca cgacgaccac caggatgccg
    14521 agtactttgc gtgacattat atttctcctc caagcccact gagcttcggt cggacaacgc
    14581 gccttcggca aatcggttgc actaccgcaa cgatcgcttt cgcttcccag ggggcgcggc
    14641 ggctggacgg ttgacgaaaa cgccgccgcc cgctatccgc gcctcctttc cggggacgag
    14701 cttcccctat tccaggacga gacaagatgt cgcgcatttg cgaactgacc ggcaagggcc
    14761 ggctggtggg tcacaacgtt tcccacgcca ataacaagac caagcgcacg ttcctgccca
    14821 atctgcagaa cgtgaccttg atgtcggacg cgctccagcg cggcgtgaag ctgcgcgtgt
    14881 cgatgagcgg cctgcgctcg atcgaacatg tcggcggcct cgacaattgg ctcgccaaga
    14941 cttcggacga gaagctcagc ctcaaggccc gccgcctcaa gcgcgagatc cggaagaagg
    15001 acgccgacgc ggcttcggcc tgaccggcat tcctactccg tcagctcgaa cttcagcagc
    15061 aaggtccgct gcagcgcgtt gaaattgtcg tccgacgcca tccagacgat ggtgcgaccg
    15121 gcctcgcgcg tgacgctcaa tgcctccata ttgtcgaccg ttacgggcgc gtcgaaatgc
    15181 gcgatctcct cgccctcgat gacgctgccg gcggcgagcc gcggcgccgc gaccgtcagc
    15241 ttggccgaaa tgccgtgcag caatgagaag cgccggttga gaaacaggat ccggccgtcc
    15301 gccagcaaag cggcatcggt cacacgatag tttttgggag ggcggtagcc gagcgtcttg
    15361 gtcggcgttc ctggtaccgc cgcgtcgccg gcaaaggcga ccacgtcgct cgagccgttc
    15421 ggcctcagcc aatgttcggc gaagaccgcg aaccgcccgt cgggaaagcg cagcaaagcc
    15481 tcgctgccgg tgttgccggg ccagcgccgc atcggcgccg gctcggcgtc cgattcggcg
    15541 cgccagctcg ggcggctgta gcgccagatg gcgttgcgcc cctcgaacgc gatccaggct
    15601 ttgtcgccgc tgaccgccag cgtctcggcg tcgcggtcga gcttgccgcg atcgccgcgg
    15661 ggtccgtcgg gcagcggccg gatcgacacc ggccccggcc gcccgggcag gccgaactcg
    15721 atcagcgacc cggagtcgct cagcgccagc acccggccgt cctcgacatg catggcggaa
    15781 atgccgccga agcgcgggtc gttgctccgg atcgaccagc cgccgagata gacgagccgg
    15841 cccagccgcc ggcgcgcgag gtgctcggtg tcgagcggca ccggatcgaa gccgatcgcg
    15901 atcagagccg gcagcggcgc gtccctgaac agcgccgggg gcgcgaaggt tgtgaccagg
    15961 gggaacgcga cgagcgcggc gagcaggcgc atggcgcccg ccctaaggtg cgtcacccgt
    16021 cgccgcaacg gccgcgaagg gggctggcgg cgccggattt cgatgaacgg g
//
LOCUS       DASVRK010000191        12280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_411758, whole genome shotgun sequence.
ACCESSION   DASVRK010000191 DASVRK010000000
VERSION     DASVRK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411758"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2118)
                     /locus_tag="VF589_07520"
     CDS             complement(<1..2118)
                     /locus_tag="VF589_07520"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX8257466.1"
                     /translation="MDNPAAAAPPPEAEAPPEAREAARSRYFNRELSWLAFNRRVLEE
                     ACNPAHPLLERLRFLSISGNNLDEFFMVRVAGLKGQQLLGVEEHSDDGLTPAQQLAAI
                     AREADQLTASQQAVWATLKLALAEAGIEVIGFGELDAESTAWLERYFRDQVFPVLTPQ
                     AIDPAHPFPFIPNKGFSLIFDLKRLADGEPIRELLMIPATLPRFVRLPGERTRYVAIE
                     TFIRRHTYLLFPGYEMLGCGAFRTIRDSDIEIEEEAEDLVRYYRSAIKRRRRGRVIRL
                     ELEADISDQLEAMVRTGIDASEALVFESSGFLGIADLELLVEEDRADLKFTPFTARFP
                     ERIREHGGDCFAAIRSKDIVVHHPYESFEVVVAFLRQAAEDPDVVAIKQTLYRAGKQS
                     AVIRALIDAAEAGKSVTAVVELKARFDEEQNLLWANALERAGVQVVYGFIDWKTHAKT
                     SMVVRREERGYRTYCHLGTGNYHPVTARIYTDISFFTADPRVGRDVAQLFNYITGYVE
                     PRDLELLVMSPHGLRDELCRLIDRECRNAEAGKPAMIWAKMNSLVDPTIIDKLYAASA
                     AGVEIDLIVRGICCLRPGVKGLSENIRVKSIVGRFLEHSRIWAFGNGEDLPNREALVY
                     VSSADWMPRNFDRRVEYMLKIENPTVHAQILDQVMVASLIDNEQSWRLLPDGNYERLQ
                     PSAEDQPFNLHRYFMTNPSLSGRG"
     gene            complement(2120..2740)
                     /locus_tag="VF589_07525"
     CDS             complement(2120..2740)
                     /locus_tag="VF589_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HEX8257467.1"
                     /translation="MNQIALPLDWPVADRDEDFLVSDANRAAFEHLRRWSLWPVMATL
                     LTGPRKSGRSLLGRIFVRKTGGRLFDDAQAHDEEALFHAWNSAQQARRPLLIIADAVP
                     PAWEVRLPDLKSRLMATPHIRIEEADDALLGDLIVKLLEDRGVAPPPDLAEYLVPRIE
                     RSYLAVQRIVDALDRAVLLHHRRMTLPMARRALTEAGMIHRARNRN"
     gene            complement(2749..4032)
                     /locus_tag="VF589_07530"
     CDS             complement(2749..4032)
                     /locus_tag="VF589_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX8257468.1"
                     /translation="MKLLRRVPPTAALAVALFLAGGGALYAQLEGADRGVPPVDSAST
                     LEITGIEVDTVGKNAEEARFEGWRQAQQLGWKALWAKTTGRPAAQAPGLPDSALNTMV
                     SGIIIEQEQVGPRRYVARLGLLFDRARTGALLGGGGEGGAIRHSAPMLVIPVMVTGSS
                     YQTFESRTAWQRAWARFRTGGSAIDYVRPTGSGIDPLLLNAAQSKRPGRAWWRILLDQ
                     YGAADVIVPTVHLKRLYPGGPAIGTFSARFGPDNQLIDRFTLRVANSAAIPRLLDEGV
                     RRLDLSFTRALNAGLLTPDPSLVIEEPAILSQIAAQIEAANAAEGGGGPAAPPPPPPS
                     SGLPAAPVPVGAAQMFSIQVDTPSSAAVSQAEISVSRVRGVTSALTTSLALGGTSVMR
                     VTFVGDSAALAAALQAQGWTVSGSGNTLRISRGGQ"
     gene            4093..5196
                     /gene="purM"
                     /locus_tag="VF589_07535"
     CDS             4093..5196
                     /gene="purM"
                     /locus_tag="VF589_07535"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX8257469.1"
                     /translation="MSESEPRESYTYAKAGVSIAAGNALVKAIGPLAKSTARPGADAS
                     LGGFGGFFDPKAAGYKDPLLVAANDGVGTKLKLAIDAGRHDGVGIDLVAMCVNDLIVQ
                     GAEPLFFLDYFATGKLDTGVAEAVVAGIAEGCRQAGCALIGGETAEMPGMYAPGDYDL
                     AGFCVGAVERGEQLTGDRVAAGDVILGLASSGVHSNGFSLVRRLAADKGWKLDRPALF
                     DQDRLLIEALLAPTRIYVKALLPVVRAGRINALAHITGGGLLENIPRVLSEGLRAFVD
                     ADAWPQPRLMAFLQAQGNIEPGEMARTFNCGIGMALVVAPDVAEAVAAELQAAGEQVF
                     AIGRIESGPRGCTVTGSAEAWSARGAWSAGHDG"
     gene            5189..5806
                     /gene="purN"
                     /locus_tag="VF589_07540"
     CDS             5189..5806
                     /gene="purN"
                     /locus_tag="VF589_07540"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX8257470.1"
                     /translation="MADTLKVAVLISGRGSNMKALIDYEREADPAYEVVLVAANVPEA
                     RGLVLARRFGIATWAMSHKGLERAEFDRLLDAELRRHRVEAIALAGYMRLLSDAFIAG
                     WEGRILNVHPSLLPLYKGLDTHRRALLAGDLYAGCSVHLVTAELDSGPVLAQARVRIG
                     ARDDADSLAERVLAEEHRLYPRALGAFARERLAERERGDGEARNG"
     gene            5784..6410
                     /locus_tag="VF589_07545"
     CDS             5784..6410
                     /locus_tag="VF589_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257471.1"
                     /translation="MAKRETAEQAEARAVRRRWITLGEVLAVAAVAISGLSFWNSYQE
                     RSSAEAERAAEKRSEAAEKQKAAKAAQTLLLKASGGGKALRLAPLDRGQTIQSQTIAF
                     PAALGVERIDTVIEPRIAAEWVERAAEKARPAERGPGDLRLPVAITTNFVSGGESHSD
                     TAIYDVGYRIDSGLLDSDVELLGLSLVERVPAAEAQRRLDSVWAARSK"
     gene            complement(6719..8884)
                     /locus_tag="VF589_07550"
     CDS             complement(6719..8884)
                     /locus_tag="VF589_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX8257472.1"
                     /translation="MHLAFKSALLPTVSLLAIASPAAAQSLEEEQASEEIIVTAQRGN
                     QTQVIRGGSVGVLGDKPAEDVPFSIKSYGETLILNQQPQTLGQVLENDPSVRTTYGFG
                     NAAEQFVIRGFTLFGDDIGLDGLYGLAPRQLVAPELYEQVQVLSGASAFLNGAAPGGS
                     GIGGSVNLIPKRAGSRPLSRATVNYTSDAHFGGSFDFARRFGDGAFGVRINGAARSGD
                     VSVEDEYRSAYVLGGAFDWEGERARLSLDLAYQRVRIRQLRPKVTIGTGVIPEVPRAD
                     ANYAQPWTYTTLRDVFGIVRGEYDLADNALLYASFGARDGSEQGVYGGITVTDAVTGA
                     ATGSALFVPRTDNNEAAQAGLRVNLAAGGVTHELNFGGSAIWQVNRNAFDFLGGFAGY
                     PTNLYDPPVVPLPGTGFVGGDLDDTFPVSRTRLASAFASDTIGLFGDRLLVTAGLRLQ
                     TIRSLSYSYTEGAETGRYDESAVTPVAGIVVKPVEGVSLFANRIQGLAQGPTAPIDPR
                     VVNSGEVFPPFKSTQYEVGGKLSLGRFNASLALFQTTQPSAFSTPVDPADPTGPLLFG
                     VNGEQRNRGIELSLDGEPVRGLRIIAGGSLIDAELRGTAGGLNDGNQAVGVPEILLNA
                     GVEWDLPFVRGATLTGRFVHTGEQQVDAANTLQIPAWSRLDLGARYVLALGDRPLTLR
                     ATIDNFANKRYWASSFDAFGAALLQGAPRTFKLSASFDF"
     gene            complement(8918..9100)
                     /locus_tag="VF589_07555"
     CDS             complement(8918..9100)
                     /locus_tag="VF589_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257473.1"
                     /translation="MTRHPFPLRKIFGVPLLIALLTFVGLVSALTGDGLRDALSWVAL
                     AVPVAAVGWAVCARRS"
     gene            complement(9097..10251)
                     /locus_tag="VF589_07560"
     CDS             complement(9097..10251)
                     /locus_tag="VF589_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX8257474.1"
                     /translation="MTRRSLHGWQLVHKWSSIVCTAFLLMLCLTGLPLIFHDEIDDLA
                     GGHGELALGCAELPHKSLDEMMRIAFAGRPHEVGLFMSFDEDRPVVNITTGPRPDAAA
                     EEMTIRSFDLRSGEMVGAIELNGVMDWLLELHTDLFLGLPGMLFLGAMGLLFLLAIVS
                     GVVLYAPFMRKLDFGTVRASRPRRVRWLDYHNLIGIVALGWTLVVGLTGVIHSLATPI
                     VDHWRSAGLADLIAPYEGQAAPAPRRLASLHAAVQSALAAAPGSRVQFVAFPGGGYSS
                     RYHYAVFLQGDTPLTKQLLTPALVDARTGRLDAVRPMPWYVQALQLSRPLHFGDYGGL
                     PLKILWAILDLFTIAILGSGLYLWLGKRGAAASRRLPEPGLPALAEMPAE"
     gene            10631..10966
                     /locus_tag="VF589_07565"
     CDS             10631..10966
                     /locus_tag="VF589_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEX8257475.1"
                     /translation="MAEAATNAVRPLRHEQGLRDDLCAALLTPRDAGEMSRLLTDLCT
                     PAEIRTIAERWHVARLLDETGLSYRDIHEGTGVSTTTIVRVARFLRQEPHQGYRRALD
                     ALREGGDAG"
     gene            10956..11846
                     /gene="hisG"
                     /locus_tag="VF589_07570"
     CDS             10956..11846
                     /gene="hisG"
                     /locus_tag="VF589_07570"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX8257476.1"
                     /translation="MLVDGTRIHIAIQKSGRLSDYSRGLLRDSGLRIQNGKNELTARV
                     ENFPADLMFVRDDDIPTFVSDGICEFGIVGENVLQEFALREAGARFEVVARLGFGRCS
                     LRIAAPEALDYRGAASLEGARIATSYPGLVRRFLDESGVEAEIVKMNGAVELAPRLGI
                     AGFVCDLVSTGATLEANGLRAVETVLDSEAVLVRTGKAVDARKADYGERLLSRIEGVL
                     ATRDSKYIMLNATEDSLAEISRILPGAEAPTIVPLHGRPGHFAVHAVCQESVFWETLQ
                     KLKAAGASAILVVPIEKMMM"
     gene            11843..>12280
                     /locus_tag="VF589_07575"
     CDS             11843..>12280
                     /locus_tag="VF589_07575"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX8257477.1"
                     /translation="MRRLRWSELGAGERAAALARPEQRSDPPLKAAVRAIVEDVRSRG
                     WAGLSAQAERIDGEAPRLVPVAPLAAQARRDLEPGQLGAIALAVRNIAAFHAGSLPAE
                     HVVETMPGLTVRKVWRAIDRVGLYIPGGRTPLFSTLLMLALPAR"
BASE COUNT         1917 a         3843 c         4677 g         1843 t
ORIGIN      
        1 gccccgcccg gaaagcgacg ggttggtcat gaagtaacgg tggaggttga acggctggtc
       61 ctcggcgctg ggctgcagcc gctcgtaatt cccgtccggc aggaggcgcc aactctgctc
      121 gttgtcgatc aggctcgcca ccatcacctg gtcgaggatc tgggcgtgga cggtcgggtt
      181 ctcgatcttc agcatatatt cgacgcggcg gtcgaaattg cgcggcatcc agtcggccga
      241 gctgacatag accagagcct cgcgattggg caggtcctcg ccattgccga acgcccagat
      301 gcggctgtgc tcgaggaagc ggccgacgat cgacttgacg cggatattct ccgaaagccc
      361 cttcacgccc ggacgcaggc agcagatgcc gcggacgatg aggtcgatct cgacgccggc
      421 ggcgctggcc gcgtagagct tgtcgatgat ggtcggatcg accagcgagt tcatcttggc
      481 ccagatcatg gcgggcttgc cggcctcggc gttgcggcat tcgcggtcga tcaggcggca
      541 caattcgtcg cgcaggccgt gcggcgacat gaccagcagt tcgaggtcgc gcggttcgac
      601 atagccggtg atgtagttga acaattgcgc gacatcgcgg ccgaccctcg gatcggcagt
      661 gaagaagctg atgtcggtat agatgcgggc cgtgaccgga tggtaattgc cggtgcctag
      721 gtggcagtaa gtgcgatagc cgcgctcttc gcgccggacg accatcgaag tcttggcgtg
      781 ggtcttccag tcgatgaagc cgtagacgac ctggacgccg gcgcgctcca gcgcattggc
      841 ccagagcaaa ttctgctcct cgtcgaaacg ggccttgagt tcgaccacgg cggtgacgga
      901 cttgccggcc tcggcggcgt cgatcagtgc gcggatgacg gccgattgct tgccggcgcg
      961 gtagagggtc tgcttgatcg cgacgacgtc gggatcctcg gccgcctggc ggaggaaggc
     1021 gaccaccacc tcgaagcttt catacgggtg gtggacgacg atgtccttgc tccggatcgc
     1081 cgcgaagcaa tcgccgccat gctcgcggat gcgctcgggg aaacgggcgg tgaagggcgt
     1141 gaatttgagg tcggcgcggt cctcctcgac cagcagttcg aggtcggcga tgccgaggaa
     1201 gccgctcgat tcgaacacca gggcctcgct cgcgtcgatg ccggtgcgga ccatcgcttc
     1261 gagctggtcg gaaatgtcgg cctcgagctc gagccggatc acgcggccgc ggcggcggcg
     1321 cttgatcgcg ctccgataat agcggaccag atcctccgct tcctcctcga tctcgatgtc
     1381 gctgtcgcgg atggtgcgga aggcgccgca gccgagcatc tcgtagccgg ggaagagcaa
     1441 ataggtgtgg cggcggatga aggtctcgat agcgacgtag cgggtgcgct cgcccggcag
     1501 gcggacgaag cgcggcaggg ttgccgggat catcagcaat tcgcggatcg gttcgccgtc
     1561 ggccagccgc ttcagatcga agatcagcga gaagcccttg ttaggaatga acgggaacgg
     1621 gtgggcgggg tcgatcgcct gcggcgtcag caccgggaag acctgatcgc gaaaatagcg
     1681 ttcgagccag gcggtcgact cggcgtcgag ctcgccgaag ccgatcactt cgatgccggc
     1741 ctccgccaga gcgagcttga gggtggccca gaccgcctgc tggctggcgg tgagctggtc
     1801 ggcctcgcgc gcgatcgcgg cgagctgctg ggccggggtg aggccgtcgt ccgaatgttc
     1861 ctcgacgccg agcagctgct ggcccttgag gccggcgacg cggaccatga agaattcgtc
     1921 gagattgttg ccggagatgg agaggaagcg cagccgctcg agcaacgggt gggcggggtt
     1981 gcaggcctct tcgagcacgc ggcggttgaa ggcgagccag ctcagctcgc ggttgaaata
     2041 gcggctgcgg gccgcttccc gggcctcggg cggcgcttcg gcctcgggcg gcggggcggc
     2101 ggcggctgga ttgtccatgt cagttccggt tcctggctcg atggatcatg ccggcctcgg
     2161 tcagcgcccg ccgcgccatc ggcaaggtca tccggcgatg gtgcagcagc acggcccggt
     2221 caagcgcgtc cacgatccgc tgaacggcga ggtagctgcg ttcgatccgc gggaccagat
     2281 attcggcgag gtcgggcggc ggcgcgacgc cgcgatcctc gagcagcttg acgatgaggt
     2341 cgccgagcag ggcgtcgtcg gcctcctcga tgcggatgtg gggcgtggcc atcagccgcg
     2401 acttcaggtc tggaagccgc acctcccagg ccggcgggac ggcgtcggcg atgatcagca
     2461 gcggccggcg cgcctgctgg gcggagttcc aggcgtggaa cagagcctcc tcgtcatggg
     2521 cctgggcgtc gtcgaacagg cggccgccgg tcttgcggac gaagatccgg ccgagcaggc
     2581 tgcggcccga cttgcgcggg ccggtgagca gggttgccat caccggccac agcgaccagc
     2641 gccggagatg ctcaaaggcg gcgcgattgg cgtcggagac caggaaatcc tcgtcgcggt
     2701 ccgccaccgg ccaatcgagc ggcagcgcga tctggttcat ggggtcggtt attgcccgcc
     2761 gcgcgagatg cggagcgtgt tgccggagcc ggagacagtc cagccctgcg cctgcagcgc
     2821 cgccgccagc gccgccgaat cgccgacgaa ggtgaccctc atcaccgagg tgccgccgag
     2881 cgcgaggctg gtggtgaggg cggaggtgac gccgcggacg cggctgaccg agatttcggc
     2941 ctggctgacc gcagccgagc tcggcgtgtc gacctggatg ctgaacattt gggcggcgcc
     3001 gaccggcacc ggggccgccg gaaggcctga gctgggcggc ggcggcggcg gcgcggccgg
     3061 gccccctccc ccttcggcgg cattggccgc ctcgatctgc gcggcgatct gcgagaggat
     3121 cgccggctcc tcgatcacca gcgacggatc gggggtgagc aggccggcgt tgagcgcgcg
     3181 cgtgaacgac aaatcgagcc ggcgcacgcc ctcgtcgagc aggcgcggga tggcggcgct
     3241 gttcgcgacg cgcagggtga agcggtcgat cagttggttg tcggggccga agcgggccga
     3301 gaaggtgccg atcgccgggc cgcccggata gaggcgcttc aggtggacgg tcggcacgat
     3361 cacgtcggcg gcgccatatt gatcgagcag gatgcgccac caggcgcggc ccgggcgctt
     3421 ggactgggcg gcgttgagca gcaaaggatc gatgccggag ccggtcgggc ggacatagtc
     3481 gatcgcgctg ccgccggtgc ggaagcgggc ccaggcgcgc tgccaggcgg tgcgcgattc
     3541 gaaggtctgg taggaggagc cggtcaccat caccgggatc accagcatcg gcgcggaatg
     3601 gcggatggcg ccgccctcgc cgccgccgcc gagcagggcg ccggtgcggg cgcggtcgaa
     3661 cagcaggccg agccgggcga cgtagcggcg cgggccgacc tgctcctgtt cgatgatgat
     3721 gcccgagacc atcgtattca gcgccgaatc gggcaggccc ggcgcctgcg cggccgggcg
     3781 gccggtggtc ttggcccaca acgccttcca gccgagctgc tgggcctggc gccagccctc
     3841 gaagcgcgcc tcctcggcat ttttgccgac cgtgtcgacc tcgatgccgg taatctcgag
     3901 cgtgctggcg ctgtcgaccg gcggcacgcc gcggtcggcc ccttcgagct gggcatagag
     3961 cgcgcccccg cccgccagga acagggcgac ggccagcgcg gcggtggggg gaacgcgtct
     4021 caaaagcttc acgacccgcc ttttggcgac agaaacgtgg aaatccaagc gcgatgtgat
     4081 taggcgcggc gcatgagcga aagcgagccg agagaatcct acacttacgc caaggccggc
     4141 gtctcgatcg cggccggcaa cgcgctggtg aaggcgatcg gcccgctcgc caaatcgacc
     4201 gcccggcccg gcgcggacgc ctcgctcggc gggttcggcg gcttcttcga ccccaaggcg
     4261 gcgggctaca aggatccgtt gctggtcgcg gcaaacgacg gcgtcggcac caagttgaag
     4321 ctcgccatcg acgcgggccg ccatgacggc gtcggcatcg acctggtcgc gatgtgcgtc
     4381 aacgacctca tcgtccaggg cgccgagccg ctcttcttcc tcgattattt cgcgaccggc
     4441 aagctcgaca ccggggtggc cgaggcggtg gtggcgggca tcgccgaggg ctgccggcag
     4501 gcgggctgcg cgctgatcgg cggcgagacg gcggaaatgc cgggcatgta tgcgccgggc
     4561 gattacgatt tggccggctt ttgcgtcggc gcggtcgagc gcggggagca gcttacgggc
     4621 gacagggtgg cggccggcga cgtgatcctc ggcctcgctt cgtcgggcgt tcattcgaac
     4681 ggtttctcgc tggtgcggcg gctcgcggcc gacaagggct ggaagctcga ccgccctgcc
     4741 cttttcgacc aggatcggct gctgatcgag gctttgctcg cgccgacccg gatctacgtg
     4801 aaggcgctgc tcccggtggt gcgggcgggc cggatcaacg cgctcgccca tatcaccggc
     4861 ggcggattgc tcgagaatat cccgcgcgtg ctgtcggagg ggctgcgcgc tttcgtggac
     4921 gccgacgcgt ggccgcagcc gcggctgatg gccttcctgc aggcgcaggg caatatcgag
     4981 cccggcgaga tggcgcgcac cttcaattgc gggatcggca tggccctggt ggtggcgccc
     5041 gacgtggccg aagccgtggc ggcggagctt caggcggcgg gcgagcaggt gttcgcgatc
     5101 ggccgcatcg agtccgggcc tcgcggctgc accgtcaccg gctcggccga ggcctggagc
     5161 gcgcgcgggg cctggtctgc gggccatgat ggctgacacg ctcaaggtcg cggtgctgat
     5221 ttcgggccgc ggctcgaaca tgaaggcgct gatcgactat gagcgcgagg cggacccggc
     5281 ctatgaggtg gtgctggtcg ctgccaacgt gcccgaggcg cgcgggctgg tgctggcgcg
     5341 gcggttcggg atcgcgacct gggcgatgag ccacaagggg ctggagcggg ccgagttcga
     5401 ccggctgctc gacgccgaat tgcgccgcca ccgcgtggag gcgatcgcgc tggccggtta
     5461 catgcggctg ttgtcggacg cgttcatcgc cggctgggag ggccggatcc tcaacgtcca
     5521 tccctcgttg ctgccgctct acaaggggct cgacacccac cggcgggcgc tgctggcggg
     5581 cgacctttat gccggctgct cggtccatct ggtgaccgcg gagctggaca gcgggccggt
     5641 gctcgcccag gcgcgggtcc ggatcggcgc ccgtgacgat gccgacagcc tggccgaacg
     5701 ggtgctggcc gaggaacaca ggctttatcc gcgggcgctg ggcgcgttcg cgcgggagcg
     5761 gctggcggag cgggagcggg gcgatggcga agcgcgaaac ggctgagcag gcggaagcgc
     5821 gggcggtccg gcggcgctgg atcacgctcg gcgaagtgct ggcggtggcg gcggtggcga
     5881 tttccggcct cagcttctgg aacagctatc aggagcgcag cagcgccgag gccgagcggg
     5941 ccgccgaaaa gcggtcggaa gcggccgaga agcagaaagc ggcgaaggca gcgcagacct
     6001 tgctcctcaa ggcgagcggc ggcggcaagg cgctgcggct ggcgccgctc gaccgcggcc
     6061 agacgatcca gagccagacc atcgcctttc cggccgcgct cggggtggag cggatcgaca
     6121 cggtgatcga gccgcgcatc gcggccgaat gggtcgagcg ggcggcggag aaggcgcggc
     6181 cggcggagcg cggcccgggc gacctcaggc tgccggtggc gatcacgacc aacttcgtca
     6241 gcggcggcga gagccacagc gacacggcga tctacgacgt cggctaccgg atcgattcgg
     6301 gcctgctcga cagcgacgtc gagctcttgg gcctgtcgct ggtcgagcgg gtcccggcgg
     6361 ccgaggcgca gcgccggctc gattcggtgt gggcggcgcg ttccaaatag ggcacggcca
     6421 aatagggatc ggtccggcgg gcgcggtgtc tcaggcgcgt gcgggtgcgg aaaaagggcg
     6481 cgcggagccc cttgttcccc tgcgaatgca ggggtccagg acggcctctc cactgggccg
     6541 cggcctccgc cggggaacga cccaggcgcc gaactgctag ggtgcggcgc cacttgttcc
     6601 cctgcgaatg caggggtcca ggccgacctc ccctctggcc ccggctttcg ccggggactg
     6661 gacccccgcc ttcgcggggg aacagtgctc gactggaccc ccgccttcgc ggggaacatt
     6721 agaagtcgaa ggaggccgac agcttgaagg tgcggggcgc gccctggagg agggcggcgc
     6781 cgaaggcgtc gaaggaggag gcccagtagc gcttgttggc gaaattatcg atggtggcgc
     6841 gcaaggtgag cgggcggtcg ccgagcgcga gcacgtagcg ggcgccgaga tcgagccgcg
     6901 accaggccgg gatctggagc gtgttggcgg catcgacctg ctgctcgccg gtgtggacga
     6961 atcggccggt cagcgtcgcg ccgcgcacga aagggagatc ccattcgacg ccggcattga
     7021 gcaggatttc gggaacgccg accgcctggt tgccgtcatt gagcccgccc gccgtgccgc
     7081 gaagctcggc gtcgatcagc gagccgccgg cgatgatgcg aagcccgcgc accggctcgc
     7141 cgtcgaggct gagctcgatg ccgcgattgc gctgctcgcc gttgaccccg aacagcagcg
     7201 ggccggtcgg atcggcgggg tcgaccggcg tgctgaacgc gctcggctgg gtcgtctgga
     7261 acagagcgag cgaggcgttg aagcggccga ggctgagctt gccgccgacc tcatattggg
     7321 tggatttgaa gggcgggaag acttcgccgg aattgacgac cctcggatcg atcggggcgg
     7381 tggggccctg ggcaaggccc tggatgcggt tggcgaacag ggacacccct tcgaccggct
     7441 tgacgacgat gccggccacg ggcgtcacgg cggattcgtc gtagcggccg gtctcggcgc
     7501 cctcggtgta ggaatagctt agcgagcgga tcgtctggag ccggaggccg gcggtcacca
     7561 gcaggcggtc gccgaacagg ccgatcgtgt cggaggcgaa ggcgctggcg aggcgggtgc
     7621 ggctgaccgg gaaggtatcg tcgagatcgc cgccgacgaa gccggtgccg ggcaacggca
     7681 ccaccggcgg atcgtagagg ttggtggggt agccggcgaa gccgccgagg aagtcgaagg
     7741 cgttgcggtt gacctgccag atcgccgagc cgccgaaatt gagttcgtgg gtgacgccgc
     7801 cggcggcgag gttgacgcgc aggcccgcct gggccgcttc attattgtcg gtgcgcggca
     7861 cgaacaaagc gctgccggtg gcggcgccgg tgacggcatc ggtgacggtg atgccgccat
     7921 agacgccctg ctcggacccg tcgcgggcgc cgaacgaggc atagaggagc gcgttgtcgg
     7981 cgaggtcata ttcgccgcgg acgatgccga acacgtcgcg caaggtcgtg taggtccagg
     8041 gctgggcgta attggcgtcg gcccgcggca cttcgggaat gacgccggtg cctatcgtga
     8101 ccttggggcg gagctggcgg atgcggacgc gctgataggc gaggtcgagc gacagccgcg
     8161 cccgctcgcc ttcccagtcg aaggcgccgc cgagcacata ggcgctgcga tattcgtcct
     8221 cgaccgagac gtcgccgctg cgggcggcgc cgttgatgcg aacgccgaac gcgccgtcgc
     8281 cgaagcggcg ggcgaaatcg aagctgccgc cgaaatgggc gtcggacgta tagttcacgg
     8341 tggcgcggga cagcggcctc gacccggcgc gcttcgggat cagattgacg ctgccgccga
     8401 tcccggaccc gccgggggcg gcgccgttca gaaaggcgct ggcgccgctc aatacctgga
     8461 cctgctcgta aagctcgggc gcgaccaatt ggcgcggcgc gaggccgtag aggccgtcga
     8521 ggccgatatc gtcgccgaac aaggtgaagc cgcggatgac gaactgctcg gcggcgttgc
     8581 caaagccgta ggtggtgcgc accgagggat cgttctcgag cacctggccg agggtttgcg
     8641 gctgctggtt gaggatcagg gtctcgccat agcttttgat gctgaacggc acgtcctcgg
     8701 cgggcttgtc gccgagcacg ccgacgctgc cgccgcggat cacctgcgtc tggttgccgc
     8761 gctgggcggt gacgataatc tcttcggagg cctgctcctc ctcaaggctc tgggcggcgg
     8821 cgggcgaggc gatggcgagc aaagagacgg tgggcagcag cgcggacttg aaagctaggt
     8881 gcaacggaaa atcctcttgg cgttcggttt tatgggttca ggagcggcgg gcgcagacgg
     8941 cccagcccac ggccgcgacc gggacggcga gcgcgaccca ggacaaagcg tcgcggaggc
     9001 cgtcaccggt gagcgcgctg acgaggccga cgaaggtcag caaggcgatg agcagcggca
     9061 cgccgaagat tttgcgcagc gggaacgggt gccgggtcat tcggcgggca tttcggcgag
     9121 ggccggaagg ccgggttcgg ggaggcggcg ggaagcggca gcgccgcgtt tgccgagcca
     9181 gaggtagagg cccgagccga ggatcgcgat cgtgaagagg tcgaggatcg cccacaaaat
     9241 cttgagcgga aggccgccat aatcgccgaa atggagcggc cgcgagagct ggagcgcctg
     9301 cacataccag ggcatcggcc gcaccgcgtc gagcctgccg gtgcgcgcgt cgaccaaagc
     9361 cggcgtcagc aattgcttgg tgagcggcgt gtcgccctgc aggaagacgg cataatggta
     9421 gcggctgcta tagccgccgc cgggaaaggc gacgaactgg acgcggcttc cgggcgcggc
     9481 ggcgagcgcg ctttggacgg cggcgtggag cgaggcgagc cggcgcggcg ccggcgcggc
     9541 ctggccttca taaggcgcga tgagatcggc gagtccggcg ctgcgccaat ggtcgacgat
     9601 cggggtggcg agcgagtgga tcacgccggt gaggccgacc accaaagtcc agccgagcgc
     9661 gacgatgccg atgagattgt gatagtcgag ccaccgcacc cgccgcgggc gggaggcgcg
     9721 gacggtgccg aaatcgagct tgcgcatgaa cggggcgtag aggaccacgc ccgagacgat
     9781 ggcgagcagg aacagcaggc ccatcgcgcc gaggaacagc attccgggca ggccgaggaa
     9841 caggtcggta tggagctcga gcagccagtc catcaccccg ttaagctcga tcgcgccgac
     9901 catctcgccc gagcgcaggt cgaacgagcg gatcgtcatc tcctcggcgg cggcgtcggg
     9961 ccgggggccg gtggtgatgt tcacgaccgg ccggtcctcg tcgaagctca tgaacaggcc
    10021 gacctcgtgc gggcggccgg cgaaggcgat gcgcatcatc tcgtcgagcg acttgtgcgg
    10081 gagttcggcg cagccgaggg cgagctcgcc atggccgccg gcgagatcgt caatctcgtc
    10141 gtggaagatg agcggcaggc cggtgaggca cagcatcaag aggaacgccg tgcagacgat
    10201 gctcgaccat ttgtgaacga gctgccagcc gtggagggag cgccgagtca ttcgagctgg
    10261 ttcgtgggtc gccgaagccg cttaccggcc ggagcgcagc ggcgcgtggc ggcctgagga
    10321 gccgcgatcg cgcgctaggc tggtgctggt cgtggacatt cgtcgtctct caatcggttc
    10381 gccgcgctgg cggggcgggc gcggctatca agccgaattg cgaatgattt gcaatagcgt
    10441 tgcttcgcct ccgcgtcatt ccagcgaaag ctggagccgg agacgaccgc aggtcaatct
    10501 cccttcttct tcccggcccg caagattcca gcttgcgctg gaatgacgag agggctgccg
    10561 cgaatcgggg ttgcgccgtc cggcaggcgc ggctagggcg tgccaatgta ctatcacgtt
    10621 gtaacagata atggctgagg cagccaccaa cgcggtgcgg ccgctccgtc acgagcaggg
    10681 gctgcgcgac gatctgtgcg cggccttgct gacgccgcgc gacgccgggg agatgagccg
    10741 gctgctgacc gacctttgca cgccggccga gatccggacc attgcggagc gctggcacgt
    10801 cgcgcggctg ctcgatgaga ccggcctttc ctatcgcgac atccatgagg gcaccggcgt
    10861 cagcacgacc accatcgtgc gggtcgcccg cttcctgcgc caggagccgc accagggcta
    10921 tcgccgcgcg ctcgacgcgc ttcgggaggg cggcgatgct ggttgacggc acgcgcatcc
    10981 acatcgccat ccagaagtcg ggccgcctgt ccgactatag ccgcgggctg ctgcgcgatt
    11041 cggggctgcg catccagaac ggcaagaacg agctcaccgc gcgggtggag aatttcccgg
    11101 ccgacctgat gttcgtgcgc gacgacgata tcccgacctt cgtcagcgac ggcatttgcg
    11161 agttcggaat cgtcggcgag aatgtgctgc aggaattcgc gctgcgcgag gccggggcgc
    11221 ggttcgaggt tgtggcgcgg ctcggcttcg ggcggtgcag cctgcggatc gcggccccgg
    11281 aggcgctcga ctatcgcggg gccgcttccc tcgagggcgc gcggatcgcg acctcctatc
    11341 ccggcctcgt ccgccgcttc ctggacgaaa gtggagtgga ggccgagatc gtcaagatga
    11401 acggggcggt cgagctggcg ccgcggctcg gcatcgccgg cttcgtctgc gacctcgtct
    11461 cgaccggcgc gacgctggag gcgaacgggc tgcgcgccgt cgagaccgtg ctcgacagcg
    11521 aggcggtgct ggtccgcacc ggcaaggcgg tggacgcgcg caaggccgat tatggcgagc
    11581 ggctgctcag ccggatcgag ggcgtgctgg cgacgcgcga ctccaaatat atcatgctga
    11641 acgccaccga ggattcactg gcggagatca gccgcatcct gccgggcgcc gaggccccca
    11701 ccatcgtccc gctgcacggc cggcccggcc atttcgcggt gcacgcggtc tgccaggaat
    11761 cggtgttctg ggaaacgttg caaaagctga aggccgccgg tgcgtcggcg atcctggtcg
    11821 tgcccattga aaagatgatg atgtgaggcg gcttcgctgg agcgagctgg gcgcgggcga
    11881 gcgcgcggcg gccttggcgc ggccggagca gcgcagcgac cctccgctga aggcggcggt
    11941 gcgcgcgatc gtcgaggatg tgcggagccg cggctgggcc gggctcagcg cccaggccga
    12001 gcgcatcgac ggggaggcgc cgcggctggt gccggtggcg ccgctggcgg cgcaggcgcg
    12061 gcgggatctg gagccgggcc agctcggggc gatcgcgctt gccgtgcgca atatcgcggc
    12121 tttccacgcg ggaagcttgc ccgccgagca tgtcgtggag acgatgccgg ggctgacggt
    12181 gcgcaaggtc tggcgcgcga tcgaccgggt cgggctctac ataccgggcg gccggacgcc
    12241 gcttttctcg actttgctga tgctcgccct ccccgcccgc
//
LOCUS       DASVRK010000192         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_347996, whole genome shotgun sequence.
ACCESSION   DASVRK010000192 DASVRK010000000
VERSION     DASVRK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347996"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VF589_07580"
     CDS             complement(<1..1719)
                     /locus_tag="VF589_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257478.1"
                     /translation="MAIIKVGDTYDTGGGHEVQIDAGHDGSIVAAGTEHLNFLYRLDE
                     DGSETSLVQTASFGQFTNGYVETLSDGRYALYATNHYGLGYGARVQLINRDGSPATEV
                     INPEFEGGEDRNGAGYTLTATSDGGFAFVWNDISRSSDAFEVTYPPNPHNGGTSSTTY
                     AGYDVRVRYFDATGTATTPSVVADDDVETANGATANRRALDQYINDSETLAGGGTAMV
                     YVDRRWAGASGGGADQEWQLSLQISTPGNVGEPVKIDLGAFGTAFGTYPDSLTPSASA
                     NIVPLPDGSFAVIWTEDTFVAAPVWGNYERTGTQTVIRYFNAAGEPLSDQVTLVTRGT
                     NHGNHRQTVWAEALSDGRIAIAYNVGVDGVNGNGSLDAFVGVVGPLGSSLEVTRVNDT
                     AAANTQFYTIKDLAVRSDDTVELVYRDVERANNTVIERFAVQDVGEVVQNGSAGVDTI
                     NGGGFQDVIFGLGGADRLAGGGGRDNLHGGDGNDGLRGGNHDDRLFGGAGNDVVEGGA
                     GADVMNGGAGRDTLSYATSDAGVAVRLANGAVSGGHAAGDSFAGFEDISGSAFDDRLS
                     GDGGVNA"
     gene            complement(1958..>2392)
                     /locus_tag="VF589_07585"
     CDS             complement(1958..>2392)
                     /locus_tag="VF589_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HEX8257479.1"
                     /translation="GGVDSLLGGDGNDRLEGGDDGDLLTGGAGIDTLAGGGGRDILYF
                     DDGDASAVRTQADRVVGHSRADLDRINLSRIDADTTEGADGDQAFIWLGAGAFSGAAG
                     ELRYQAINGDAYLQGDTDGDGVANFFIRVDDFTTLMRGDLVV"
BASE COUNT          352 a          806 c          823 g          411 t
ORIGIN      
        1 cgcgttgacg ccgccgtcgc ccgacagccg gtcgtcgaag gccgagccgg agatgtcctc
       61 gaagccggcg aagctgtcgc cggcggcgtg gccgcccgag accgcgccgt tggccagccg
      121 caccgccacg cccgcgtccg aggtcgcgta gctcaaggtg tcgcggccgg cgccgccgtt
      181 catgacgtcg gcgcccgcgc cgccttcgac cacgtcgttg ccggcgccgc cgaacaggcg
      241 gtcgtcgtga ttgccgcctc taaggccgtc attgccatcg ccgccgtgga gattgtcgcg
      301 gccgccgccg ccggcgagcc ggtccgcgcc gccgaggccg aagatcacgt cctgaaagcc
      361 gccgccgttg atcgtgtcga ccccggcgga gccgttctgc acgacctcgc ccacatcctg
      421 caccgcgaag cgctcgatca cggtgttgtt ggcgcgctcg acgtcgcggt agacgagctc
      481 gaccgtgtcg tcgctgcgca cggcgaggtc cttgatggtg tagaattggg tgttggccgc
      541 ggccgtgtca ttgacccgcg tcacttcgag cgaggagccg agcggaccga cgactccgac
      601 gaatgcgtcg aggctgccgt tgccgttcac gccatccacg ccgacattgt aggcgattgc
      661 gatccgcccg tccgacagcg cctccgccca cacggtttgg cggtggttgc cgtggttggt
      721 gccgcgggtg acgagggtga cctggtcgct cagcggctcg ccggcggcgt tgaaatagcg
      781 gatcaccgtc tgggtgccgg tcctttcgta attgccccac acgggcgcgg cgacaaaggt
      841 gtcctcggtc cagatcacgg cgaagctgcc gtccggcaga gggacgatgt tcgcgctcgc
      901 ggacggggtc agcgagtccg gataggtgcc gaacgcggtc ccgaacgccc cgagatcgat
      961 cttgaccggt tcgccgacat tgcccggcgt ggagatctgc agcgagagtt gccactcctg
     1021 atcggcgccg ccgcccgatg cgcccgccca gcgtctgtcg acatagacca tagccgtgcc
     1081 gccgccggcc agggtctcgc tgtcgttgat atattgatcg agagcgcgcc ggttggcggt
     1141 cgctccattc gccgtttcga cgtcgtcgtc ggcgacgacc gagggggtgg tggcggtgcc
     1201 ggtcgcgtcg aaataacgga cgcgaacgtc atagccggcg taggtggtgc tgctggtgcc
     1261 cccattgtgg gggttgggcg gataagtcac ctcgaacgcg tcgctggacc ggctgatgtc
     1321 gttccacacg aaagcgaagc cgccgtcgga ggtggcggtg agggtatagc ccgccccgtt
     1381 gcgatcctcg ccgccttcga actccgggtt gatgacctcg gtcgcggggc tgccgtcgcg
     1441 gttgatgagc tggacgcgcg caccgtagcc gaggccataa tggttggtcg cgtagagcgc
     1501 atagcgtccg tcgctgagcg tttcgacata gccgttggtg aactggccga agctcgccgt
     1561 ctgtacgagc gaggtttcgg agccgtcctc gtcgagcctg tagaggaagt tcagatgctc
     1621 ggtgccggcc gcgacgatcg agccgtcatg gccggcgtcg atctggactt catgcccacc
     1681 gcccgtgtca taggtatcgc cgactttgat gatcgccatg tcgcatcccc cccttggttg
     1741 ttcgaagcct caagagcgcg aggccggtcc agcatccccg ctgctggccc cccgctcgta
     1801 cccccgcatt gcgcttgccg tagaagcaag cccgtccgcc gaacatccgc cctgaaggga
     1861 cagcaaggga cattacgggg cactgatcga aaggaccgcc aaagcctcgc caaaataaag
     1921 gcttaggagc tggacgtcgg atcactgcct gccaccctca gaccaccagg tcgccgcgca
     1981 tcagggtggt gaaatcgtcc acccggatga agaagttggc gacgccgtca ccgtccgtgt
     2041 cgccctgcag gtaggcgtcg ccgttgatcg cctggtagcg aagctcgccc gccgcgccgc
     2101 tgaaagcgcc ggcgccgagc caaatgaagg cctggtcgcc gtccgcgcct tcggttgtgt
     2161 cggcgtcgat ccggctgaga ttgatgcgat cgaggtcggc gcggctgtgc ccgaccacgc
     2221 ggtcggcctg ggtgcgcacg gcggaagcgt cgccgtcgtc gaaatagagg atgtcgcgcc
     2281 cgccgccgcc ggccagggtg tcgatgccgg cgccgcccgt gagcaggtcg ccgtcgtcgc
     2341 cgccctcgag ccggtcgttg ccgtcgccgc cgagcaggct gtcgacgccg cc
//
LOCUS       DASVRK010000193         5507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_348048, whole genome shotgun sequence.
ACCESSION   DASVRK010000193 DASVRK010000000
VERSION     DASVRK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348048"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VF589_07590"
     CDS             complement(<1..1728)
                     /locus_tag="VF589_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257480.1"
                     /translation="MTIVRVGEVVDTGAGHRHRIDAGRDGSLVVISTLDGGYLSRIDE
                     DGSTVLLQAAPSNGNLRSGEIDTLSDGRYVVSVGGHAGFGSGALVQILDRDGSPATGL
                     IDAMVDDASRYGPGFTVTGTSDGGFAFVWNDYSAGSQQYQATHANGAIANYSGASDVR
                     IRYFDSNGTGALSTVADDDVESLSGVAVSRRAADQYVNDTETLAGGQTVFAYMDHRVV
                     GSPDGNGSHGEWEVSVQIAAPGNVGEPIKVDLGPYNEQSGPGTSPYPQAVDPAAGVNL
                     VAMPDGGFAALWTENTYAASSQGGGYTHTGWQTVIRYFDAAGNPLTDAITLVTRGLEH
                     GNHSKYVWAEAMSDGRIAIAYNVGTSGANGNGTLDAFLGVVGPLGSSIETTRVNQTAA
                     TNTQFYTVSDLAVRSDDTIELVYQNALVGANGHANHTLIERFAVGDAGEVVQNGSAGA
                     DTINGGGFQDVIFGLGGADRLAGGGGRDNLHGGDGNDGLRGGNHDDRLFGGAGNDVVE
                     GGAGADVMNGGAGRDTLSYATSDAGVAVRLANGAVSGGHAAGDSFAGFEDISGSAFDD
                     RLSGDGGVNA"
     gene            complement(2224..2622)
                     /gene="apaG"
                     /locus_tag="VF589_07595"
     CDS             complement(2224..2622)
                     /gene="apaG"
                     /locus_tag="VF589_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX8257481.1"
                     /translation="MKSLFPHAATTADVTVRVAVSFLPEQSEPVKGRWFWAYHVRIEN
                     EGQRAVQLVSREWTIVDGRGGRHEVAGEGVVGEQPVLEPGASFDYVSGCPLNTPSGHM
                     EGRYRMVGADGRTFTVAIPRFPLIAPTVTS"
     gene            complement(2654..3385)
                     /gene="recO"
                     /locus_tag="VF589_07600"
     CDS             complement(2654..3385)
                     /gene="recO"
                     /locus_tag="VF589_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX8257482.1"
                     /translation="MRLETQAIMLAVRPHGEHGAIVRALTPSDGVQPGFVRGGRSRRL
                     RPVLLPGNLIQAEYRSRTDEQLAQLSVELVHSRAPLLAEPLPAAAIDWATALTASALP
                     EGLAYPRLYQALEAVLDAVEAAPSAQGWATALVRYELLLLGELGFGLDLSECAATGAT
                     RDLAFVSPRSGRAVSQAGAGEYRDRLLPLPSFLLEGGLAGWGDVMDGLRLTGHFLARD
                     LLIERQADILAARERLVERLRRVTR"
     gene            3551..4366
                     /locus_tag="VF589_07605"
     CDS             3551..4366
                     /locus_tag="VF589_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX8257483.1"
                     /translation="MAVANPITAVSEAAPAPVRKITREDLRISLRDGWRDFMAMRGDL
                     IFVGLLYPLIGLVAATITLGGTMLPLFFPIAAGISLLGPIVAIGFYELARRREDGLES
                     DWTHFFDVRKRPSADGISAVAALLITIFAVWLAVAAALYVALWGPYVPNSIGEFLARL
                     FTTPQGWAMIVVGNLIGLGFAIVVLATSVVSLPMLVDCEVNASTAMSTSIAAFRTNFG
                     LMLRWGLLVAALLVIGSIPLFIGLALVLPWLGYSTWHLYTRLVDRDSFPHCTD"
     gene            complement(4518..4946)
                     /gene="rplS"
                     /locus_tag="VF589_07610"
     CDS             complement(4518..4946)
                     /gene="rplS"
                     /locus_tag="VF589_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX8257484.1"
                     /translation="MNLIQQLEAEAIEQFKTTKTIPEFRPGDTVRVGVRVVEGERTRV
                     QNYEGVCIARANRGLGSSFTVRKISFGEGVERVFPLYSPNIDSIEVVRRGAVRRAKLY
                     YLRGRTGKAARIAERRDTRHGPEAKAPAATATPETETAEG"
     gene            complement(4951..>5507)
                     /locus_tag="VF589_07615"
     CDS             complement(4951..>5507)
                     /locus_tag="VF589_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX8257485.1"
                     /translation="MVMRADVLARAVDRALEVRPEVPLLAMTPRGEPLTQALVRELAA
                     GPGVSILCGRFEGIDERLFEARTIRSVSVGDYVLSGGETAAIVLLDACVRLLPGVMGA
                     ASSGDEESFESGLLEYPHYTRPQEWEGRTIPEVLRSGDHAKIAAWRKQKAAADTRSRR
                     PDLMERHGDAPRPSPSGARHEDEG"
BASE COUNT          797 a         1879 c         1927 g          904 t
ORIGIN      
        1 cgcgttgacg ccgccgtcgc ccgacagccg gtcgtcgaag gccgagccgg agatgtcctc
       61 gaagccggcg aagctgtcgc cggcggcgtg gccgcccgag accgcgccgt tggccagccg
      121 caccgccacg cccgcgtccg aagtcgcgta gctcaaggtg tcgcggccgg cgccgccgtt
      181 catgacgtcg gcgcccgcgc cgccttcgac cacgtcgttg ccggcgccgc cgaacaggcg
      241 gtcgtcgtga ttgccgcctc taagtccgtc attgccgtcg ccgccgtgga gattgtcgcg
      301 gccgccgccg ccggcgagcc ggtccgcgcc gccgaggccg aagatcacgt cctgaaagcc
      361 gccgccgttg atcgtgtccg ccccggccga gccgttctgc accacctcgc cggcgtcgcc
      421 gaccgcgaag cgctcgatca gggtgtggtt ggcatgaccg ttcgccccca ccagcgcgtt
      481 ctgatagacg agctcgatcg tgtcgtcgct gcggacggcg agatcggaga cggtgtagaa
      541 ttgggtgttg gtagcggcgg tctggttgac gcgcgtcgtc tcgatcgagg aaccgagcgg
      601 gccgaccacg ccgaggaagg cgtcgagcgt gccgttgccg ttggcgccgg acgtcccgac
      661 attgtaggcg atcgcgatcc gcccgtccga catggcctcg gcccagacat atttgctgtg
      721 attgccgtgc tccaggccgc gggtgacgag ggtgatcgcg tcggtgagcg ggttgccggc
      781 ggcgtcgaaa tagcggatca ccgtctgcca gccggtgtgc gtatagcctc ccccttggga
      841 ggaggcggca taggtgtttt ccgtccacag agcggcgaag ccgccgtccg gcatcgccac
      901 caggttgacg cccgcggccg ggtccaccgc ctgcggatag ggcgaagttc ccgggccgct
      961 ctgctcgtta tacggtccga gatcgacctt gatcggctct ccgacattgc cgggcgcggc
     1021 gatctggacg gagacttccc actcaccgtg cgacccgttg ccgtcggggc tgccgacgac
     1081 gcgatggtcc atataggcga agacggtctg cccgcccgcg agggtctcgg tatcgttcac
     1141 atattgatcg gcggcgcgcc gggagaccgc gacgcccgag aggctctcga catcgtcgtc
     1201 cgcgacggtg gagagggcgc cggtaccgtt cgagtcgaaa tagcggatcc ggacgtcgga
     1261 cgcgcccgaa tagttcgcaa tggcgccgtt ggcgtgggtg gcctgatatt gctgcgaccc
     1321 ggcgctatag tcgttccaga cgaaggcgaa gccgccgtca ctggtgccgg tgacggtgaa
     1381 accggggccg tagcgggacg catcgtcgac catcgcgtcg atgagacccg tcgccgggct
     1441 gccgtcgcgg tcgaggatct ggaccagggc gccggagccg aagccggcat ggccgccgac
     1501 gctgacgacg tagcggccgt cgctgagcgt gtcgatttcg ccggagcgga ggttgccgtt
     1561 tgaaggcgca gcctggagca agaccgtcga gccgtcttcg tcgatccggg atagatagcc
     1621 gccgtccaac gtgctgatga ccaccagcga gccgtcgcgg cccgcgtcga tcctgtgccg
     1681 gtgcccggcg cctgtatcga caacctcgcc tacccgaacg atcgtcattg aatttctccc
     1741 ccgctcggcg cccgccatgg gcgctctcat cacagggtaa aggcgcggtc cgtcgcccgg
     1801 agaggtcagc ccggtcggaa ttatttcaag ggatcgcggt tttccggttc tgcgcccctg
     1861 cgaaggcagg ggcccagacc aggcgaaggc ccgccagcca agtctcgtag acgtcgcgcg
     1921 gggccgggac cgtgtgcggt gccaagcttt cacggccgct gcgatgaccg ggctgccgct
     1981 gaagcgccag cccgggactg gacccctgcc ttcgcagggg aacaggtgcg gcactcgcgc
     2041 ccctgcgaag gcaggggtcc agaccgggcg aaagcccgcc gctctcgtgg agaaaagccc
     2101 gccgctctgg tggacatgcg ccggccgcga ccctgtgtcc tccggcgcca ggattccaca
     2161 gccgctgcga tgaccgggct cagcccgggt ctgggcccct gccttcccag gggcacgaca
     2221 ccgtcagctg gtgacggtgg gggcgatcag tgggaagcgg gggatggcga ccgtgaaggt
     2281 acggccgtcg gcgccgacca tgcggtagcg gccttccatg tggccgctcg gcgtgttcaa
     2341 tgggcagccg gagacataat cgaacgaggc gccgggctcg agcaccggct gctcgccgac
     2401 cacaccttcg ccggcaactt cgtggcgtcc gccgcggccg tcgacgatgg tccattcgcg
     2461 gctgaccagc tggaccgcgc gctggccctc attctcgatg cggacatgat aggcccagaa
     2521 ccagcggccc ttcaccggct ccgactgctc gggcaggaag ctgacggcga cgcggacggt
     2581 gacgtcggcg gtggtggcgg catgggggaa cagggatttc acgggggcga ctttccactt
     2641 tcctctggcc ccgtcaacgc gtgacccgcc gcagccgctc caccagccgc tcgcgcgcgg
     2701 caagaatatc ggcctgacgc tcgatcagca ggtcgcgggc gaggaaatgg ccggtgagcc
     2761 ggagcccgtc catgacgtcg ccccacccgg ccaggccgcc ttcgagcagg aacgatggca
     2821 aggggagcag gcggtcgcga tactcgccgg cgcctgcctg gctgaccgcg cggccgctgc
     2881 gcgggctgac gaaagcgagg tcgcgggtcg ctccggtggc ggcgcattcg gataaatcga
     2941 ggccaaagcc gagctcgccg agcaggagca gctcgtagcg cacgagcgcg gtcgcccagc
     3001 cctgggcgct cggcgccgcc tcgaccgcgt cgagcacggc ctccagcgcc tggtagaggc
     3061 gcggataggc gagcccctcg ggcagagcgg acgcggtcag cgccgtcgcc cagtcgatcg
     3121 ccgcggccgg cagcggctcg gccagcagcg gggcgcggct atgcaccagc tcgaccgaca
     3181 attgcgcgag ctgctcgtcg gtgcgggatc gatattcggc ctggatgagg ttgcccggca
     3241 gcagcaccgg cctcagccgg cgcgaccggc cgccgcggac gaagcccggc tggacgccgt
     3301 cggacggggt cagcgcgcgg acgatcgcgc catgctcgcc atgcggccgg acggcgagca
     3361 ttatggcctg ggtttcgagc cgcatcgccc cagcctaggg caaagccgcc gcgctgagta
     3421 gggagcgggc ggcggcggat ggttcgaaaa ccggctcgca ggcgccgccc ggctctgcta
     3481 tagcggccgc aggcggcctc cggctcgcct tttcagccgg acggccgcac ctctggagag
     3541 gaggatgcca atggctgtcg ctaatcccat taccgccgtt tccgaagccg cgccggcgcc
     3601 ggtccgcaag atcacccgcg aggatctcag gatttccctc cgcgacgggt ggcgggactt
     3661 catggcgatg cgcggcgacc tgatcttcgt cggcctgctc tacccgctga tcggcctcgt
     3721 cgctgcgacg atcacgctcg gcggaacgat gctgcctttg ttcttcccga tcgcggccgg
     3781 catctcgctg ctgggcccga tcgtcgcgat cggattttac gagctggcgc ggcggcgcga
     3841 ggacgggctg gaatcggact ggacgcactt tttcgacgtg cgcaagcggc cgtcggcgga
     3901 cggcatcagc gcggtcgccg ctttgctgat caccatcttc gcggtctggc tcgcggtcgc
     3961 ggcggcgctc tacgtcgctc tgtggggccc ctatgtgccc aattcgatcg gcgagttcct
     4021 cgcccggctg ttcaccacgc cgcagggctg ggcgatgatc gtggtcggca atctgatcgg
     4081 gctcggcttc gcgatcgtgg tgctcgccac cagcgtcgtg tcgctgccga tgctggtcga
     4141 ttgcgaggtc aatgccagca ccgccatgtc gacctcgatc gccgccttcc gcaccaattt
     4201 cgggctgatg ctgcgctggg gcctgctggt cgcggccttg ctggtgatcg gatcgatccc
     4261 gctgttcatc ggcctcgctt tggtgctgcc gtggctcggt tactcgacct ggcatctcta
     4321 cacgcggctg gtcgaccgcg attccttccc gcactgcacc gattagggtc cgatttgggc
     4381 tgagttggcc gcattgctcc ccggcgaagg ccggggtcca gcccgaccat cctctgggcc
     4441 ccggccttcg ccggggaaca aaagaaaggg gcggccccga acgggaccgc cctatttctt
     4501 tgccgatgcc gtgaggctca gccctcggcg gtttccgttt cgggcgtggc ggtcgcggcg
     4561 ggagccttgg cttcggggcc gtggcgcgtg tcgcggcgct cggcgatacg cgccgccttg
     4621 ccggtgcggc cgcgcagata atagagcttc gcccggcgca ccgcgccccg acgaacgacc
     4681 tcgatcgaat cgatgttcgg cgaataaagc gggaagacgc gctcgacgcc ctcgccgaag
     4741 ctgatcttgc ggacggtgaa ggagctgccg aggccgcgat tggcgcgggc gatgcagacg
     4801 ccttcgtaat tctggacgcg ggtgcgctcg ccctcgacca cgcggacgcc gacgcgcacg
     4861 gtatcgccgg ggcggaattc ggggatggtc ttggtcgtct tgaactgctc gatggcctcg
     4921 gcctcgagct gctggatgag gttcatttct ctagccttcg tcttcgtgcc gcgcgccaga
     4981 gggcgatggc ctgggtgcgt ccccatgacg ctccatcagg tccggccgcc ttgaccgtgt
     5041 gtctgccgcc gccttctgct ttcgccaggc ggcgatcttc gcatgatccc ccgatcgcag
     5101 cacttcaggg atcgtgcgcc cttcccactc ctgaggtcgg gtataatgag gatattcgag
     5161 gaggccgctt tcgaagcttt cttcgtcacc gctggaagcc gcgcccatta ccccggggag
     5221 cagccgaacg caagcatcga gcagcacgat cgccgccgtc tcgccgcccg aaagcacgta
     5281 atcgccgacc gatacggaac ggatcgtccg cgcctcgaac aggcgctcgt cgatcccttc
     5341 gaaacggccg cacaggatcg agacgccggg gcctgcggca agctcccgga ccagcgcctg
     5401 ggtgagcggc tcgccgcgcg gggtcatcgc cagcagcggg acttcgggcc ggacctccag
     5461 cgcccggtcg acggcgcggg cgaggacatc ggcgcgcatc accatcc
//
LOCUS       DASVRK010000194         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_348341, whole genome shotgun sequence.
ACCESSION   DASVRK010000194 DASVRK010000000
VERSION     DASVRK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348341"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(388..1434)
                     /gene="queG"
                     /locus_tag="VF589_07620"
     CDS             complement(388..1434)
                     /gene="queG"
                     /locus_tag="VF589_07620"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX8257486.1"
                     /translation="MDGKAKLREALEAEARALGFAAFGVARADAAPLTAARLREWIAG
                     GAHGDMIWMEETAERRGSPAALWPEVRSVISLGMSYAPATDPLALAGEPERGRISVYA
                     QGADYHDVIKKALKALARWLVAQGDCELKVFVDTAPVMEKPLAEAAGLGWQGKHTNLV
                     SRADGSWLFLGAIYTNLELEPDRAHRAHCGSCNACLAACPTNAFPAPYRLDARRCISY
                     LTIEHKGPIPHEFREAIGNRIYGCDDCLAVCPWNRFADAARRNKAFAPRAELAAPALA
                     DLLALDDAAFRKVFAGSPIKRIGRNRMVRNAAIAAGNSGSAALVEPLRRLTADGDPAV
                     AEAAQWALERLPLR"
     gene            1484..2428
                     /locus_tag="VF589_07625"
     CDS             1484..2428
                     /locus_tag="VF589_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8257487.1"
                     /translation="MGLAIEAKGLVKAFDGKIAVDGIDIAVPEGSIYGVLGPNGAGKT
                     TTLRMLLGIIDPDRGSRTLLGHARPMEVASRVGYLPEERGLYPAMTSRDAIAFMGALR
                     GLPLDAGRKRATILLNDNGLADYVAKPIRTLSKGMAQTVQLLGTIVHRPKLIVLDEPF
                     SGLDAINQGRLEHLIRAEAAAGATIIFSTHVIAHAERLCERIAIIAGGKIRFEGLVSE
                     ARERLRPQVRLRARAAEGAWRLALPADAELDAGVWHFELPDRGIEPLLKALIDGGAGI
                     EELSIERPGLHDAFVAIAGAAAAHQMRDGLDDDLGEAA"
     gene            2425..3681
                     /locus_tag="VF589_07630"
     CDS             2425..3681
                     /locus_tag="VF589_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8257488.1"
                     /translation="MTRLFAAAWVIARRDFVATVWSRSFLIFLLAPILAMGFGGLIAE
                     LTSKADVAAARTVVAVAMPREDAAALRAAHDRLREALGRGPLADLEYVEPAADPGRHA
                     RKLLSSQEQGVSAVLSGSLARPTLTGPVRAIEGIEPELALLIEDTRRAAAIRGAGAAV
                     APVAIARVATAESRGGMNRVQHGLARGSQLLVFFLTLMLATMLLSNLVEEKSNKVIEV
                     LAAAVPLDAVFLGKLIAMLGVSVVGILVWGAIAAPILLLVQESFSVPVTPALGWPAFC
                     VLLVVYFAANYMLLGAVFLGIGGQASNVREIQTLSMPVTFAQVIIFLLASMVVGDNGG
                     TVTWVAAIFPLSSPLSMIALAAQSGSLWPHLLAILWQLAWIFLVIRLAARMFRLTVLK
                     SGGGDSFFAIFKSARRKGAPPPAGGG"
     gene            complement(3830..4207)
                     /locus_tag="VF589_07635"
     CDS             complement(3830..4207)
                     /locus_tag="VF589_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8257489.1"
                     /translation="MARITGLGGIFYKAADPARTQAWYQETLGVGGEWGAMFPWKQDQ
                     TGEAYSLLSPFKPETDYFAPSEAGFMINFRVDDLAAFLEELRAKGVEILGTQDEDYGK
                     FAWILDCDGIKVELWEQVGPAPA"
     gene            complement(4220..4987)
                     /locus_tag="VF589_07640"
     CDS             complement(4220..4987)
                     /locus_tag="VF589_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX8257490.1"
                     /translation="MGSVSQKAALFAALHRPGDPLILFNVWDAGSARAVAEGGAKAIA
                     TGSHSVAEAHGFGDAEMLPLELVLANAARIVGAVDLPVTVDFEGAYATEPEVGAENIR
                     RLAETGAVGCNLEDQVVGGQGLHPTALQAERIRAARRAAGSEFFINARTDMFLKAKAD
                     THDEGMVEAALERARAYADAGASGFFAPGLVDLRLLGQLCAASPLPVNFIAIPGAPGA
                     AEVAAAGVARISHGPFPYRAAMKALEAAAREAHGLTG"
BASE COUNT          741 a         1879 c         1658 g          808 t
ORIGIN      
        1 cgcgttgaac cagcgctaag accggcaagc gcgccagagc gttgaagggt cgaacggaat
       61 cgccatcatc cgttcgccct gagcctgtcg aagagggccc tgcgggctgc ggcgccgagc
      121 ttccacggcc gctgcgataa ccgggctgcc gctagagcgt cagcccgggg ctgggcccct
      181 gccttcgcag gggcacgggg ggtttgccgt tcctgtgccc ctgcggaggc aggggcccag
      241 cccggcccct gcgaaggcag gggccgccaa ccaagcgctc ttaggcgtcg cgcgggtcga
      301 gcccttcttc ttgttctgag gcgaataaga aagagcttcg acaggctcag cccgaacggc
      361 aacagggaca acttaccttt caacgctcta gcgtaggggc aagcgctcca gcgcccattg
      421 cgccgcctcc gcgaccgcgg ggtcgccatc cgccgtcagc cgccgcaacg gctccacgag
      481 cgccgcgctg ccgctgttgc cggcggcgat cgccgcgttg cggaccatcc ggttgcgccc
      541 gatccgcttg atcggcgagc cggcgaacac cttgcggaac gcggcgtcgt ccagcgccag
      601 caggtcggcc agcgccggcg cggcgagctc ggcgcggggc gcgaacgcct tattgcggcg
      661 ggcggcgtcg gcgaaccggt tccacgggca gaccgcgagg caatcgtcgc agccgtagat
      721 gcggttgccg atcgcttcgc ggaattcgtg cgggatcggc cccttatgct cgatggtgag
      781 gtaggagatg cagcgccgcg catcgagccg gtagggggcg gggaaggcat tggtggggca
      841 ggcggcaagg caggcgttgc agctgccgca atgggcccgg tgcgcccggt ccggctccag
      901 ctccaaattg gtatagatgg cgccgaggaa cagccagctg ccgtcggcgc ggctgaccag
      961 gttggtgtgc ttgccctgcc agccgagacc ggccgcttcc gccagcggct tctccatcac
     1021 cggcgcggtg tcgacgaaca ccttgagctc gcaatccccc tgcgcgacca gccagcgcgc
     1081 cagcgccttc agcgccttct tgatcacgtc gtgataatcg gcgccctgag cgtagaccga
     1141 gatccgcccc cgctcgggct cgccggccag cgccagcgga tcggtcgccg gcgcatagct
     1201 catgccgagc gagatcaccg atctcacctc cggccacagc gccgccggcg agccgcgccg
     1261 ctccgccgtc tcctccatcc agatcatgtc gccatgggcg ccgccggcga tccattcgcg
     1321 cagccgcgcg gcggtcaggg gcgccgcatc ggcgcgcgcg acgccgaacg cggcgaagcc
     1381 cagcgccctc gcctcggcct ccagcgcctc gcgcagcttt gccttgccat ccatcgcgcg
     1441 gtgccgctac cacattgaaa gccttagggg gacgggcaaa cttatggggc tggcaatcga
     1501 ggcgaagggt ctggtcaagg ccttcgacgg gaaaatcgcc gtcgacggca tcgacatcgc
     1561 cgttcccgaa ggcagcatct acggcgtgct cggccccaac ggcgccggca agaccaccac
     1621 cttgcgcatg ctgctcggca tcatcgatcc cgaccggggc agccgcaccc tgctcggcca
     1681 cgcccggccg atggaggtcg catccagggt cggctacctg cccgaggagc gcggcctcta
     1741 cccggcgatg acatcgcgcg acgcgatcgc cttcatgggc gcgctgcgcg ggctgccgct
     1801 cgacgccggc cgcaagcgcg cgaccatctt gcttaacgac aacggcctcg ccgattatgt
     1861 cgccaagccg atccgcactt tgtccaaggg catggcccag accgtccagc tgctcggcac
     1921 catcgtccac cgcccgaagc tgatcgtgct cgacgagccc ttttcgggcc tcgatgcgat
     1981 caaccagggc cggctcgaac atctcatccg ggccgaggcg gccgcgggcg cgacgatcat
     2041 cttctcgacc catgtcatcg cccatgccga gcggctgtgc gagcggatcg cgatcatcgc
     2101 cggcggcaag atccgcttcg aaggtttggt gtcggaagcg cgcgagcggc tgcggccgca
     2161 ggtgcggctg cgcgcccgcg ccgccgaggg cgcgtggcgg ctggcgctgc cggccgatgc
     2221 cgagctcgac gccggcgtct ggcatttcga gcttcccgac cgcggcatcg agccgttgct
     2281 gaaggcgctg atcgacggcg gcgcggggat cgaggaactg tcgatcgaac ggcccggcct
     2341 ccacgacgcc ttcgtcgcca tcgccggcgc cgccgccgcc caccagatgc gcgacggcct
     2401 ggacgacgat ctcggggaag ccgcttgacc cgcctattcg ccgccgcctg ggtgatcgca
     2461 cgccgcgatt tcgtggccac cgtctggtcg cgcagcttcc tcatcttcct gctcgcgccg
     2521 atcctcgcca tgggcttcgg cgggctcatc gcggagctca ccagcaaggc cgacgtcgcc
     2581 gccgcccgca cggtcgtcgc cgtggcgatg ccgcgcgaag acgccgccgc cctccgcgcc
     2641 gcgcacgacc ggctaaggga agcgctgggc agggggccgc tcgccgacct cgaatatgtc
     2701 gagccggccg ccgatcccgg ccgccatgcc cgcaagctgc tgtcgagcca ggagcagggc
     2761 gtctccgccg tcctcagcgg cagcctcgcc cggccgacgc tgaccgggcc ggtgcgggcg
     2821 atcgagggca tcgagccgga gctcgccttg ctgatcgagg acacccgcag ggccgcggcg
     2881 atacgcggcg ccggcgcggc cgtcgctccg gtggccatcg ccagggttgc gacggcggaa
     2941 tcgcgcggcg gcatgaacag ggtccagcac ggcctcgccc gcggctcgca attgctggtc
     3001 ttcttcctca ccctgatgct cgccaccatg ctgctgtcga acctggtcga ggagaaatcg
     3061 aacaaggtga tcgaggtgct cgccgccgcg gtgccgctcg acgccgtctt cctcggcaag
     3121 ctcatcgcca tgctcggcgt gtcggtggtc ggcatcctcg tctggggcgc gatcgccgcg
     3181 ccgatcctgc tgctggtgca ggaatcgttc agcgtgcccg taacgcccgc gctcggctgg
     3241 cccgctttct gcgtgctgct ggtggtctat ttcgcggcca attacatgct gctcggcgcc
     3301 gtcttcctcg gcatcggcgg ccaggccagc aacgtccggg agatccagac gctgtcgatg
     3361 ccggtcacct tcgcgcaggt gatcatcttc ctgctcgcct cgatggtggt cggcgacaat
     3421 ggcggcaccg tcacctgggt cgcggccatc ttcccgttga gctcgccgct cagcatgatc
     3481 gcgctcgccg cccagtccgg ctcgctgtgg ccgcatctcc tcgccatcct gtggcagctc
     3541 gcctggattt tcctggtcat ccgcctcgcc gcgcgcatgt tcaggctgac cgtgctgaaa
     3601 tcgggcggcg gcgactcctt cttcgccatc ttcaagtccg cccgccgcaa gggggccccg
     3661 ccgccggcgg gtggcgggtg atcaggttga tctgacccgc tcatcctgag gagccggtga
     3721 gcgaagtcga agcggcgtct cgaaggacgc actgggtcct tcgagagggg ccttcgccgg
     3781 ggctcagtcc ctcctcagga tgaaggaatt cccaagcccg ccaaattgcc taggccggtg
     3841 ccggcccgac ctgctcccaa agctccacct tgatgccgtc gcagtcgagg atccaggcga
     3901 acttaccata atcctcatcc tgggtgccga gtatctcgac ccccttcgcc ctcagttcct
     3961 cgagaaacgc ggcaagatcg tccaccctga agttgatcat gaaccccgct tcgctgggcg
     4021 cgaaatagtc ggtctccggc ttgaacggcg aaagcaggct gtaggcctcg cccgtctggt
     4081 cttgcttcca ggggaacatc gcgccccatt cgccgccgac gccgagcgtc tcttgatacc
     4141 aggcctgcgt ccgggccgga tcggcggcct tgtagaagat accgcccagc cccgtgatcc
     4201 tcgccatcgc cgcctccctt tagcccgtca gaccatgcgc ctcccgcgcc gccgcttcca
     4261 gcgccttcat cgccgcccgg taggggaagg gaccgtggct gatccgcgcc acccccgcgg
     4321 ccgccacctc cgcggcgccc ggcgcgccgg gaatggcaat gaagttgact ggcagcggcg
     4381 aggccgcgca cagctgcccg agcaggcgca gatcgacgag ccccggcgcg aagaagccgc
     4441 tcgctcccgc gtcggcataa gctcgcgccc gctccagcgc cgcctcgacc atcccctcgt
     4501 catgcgtgtc cgccttggcc ttgaggaaca tgtcggtccg cgcgttgatg aagaactcgc
     4561 tccccgccgc gcggcgggcg gcgcggatcc gctcggcctg gagcgcggtc ggatggaggc
     4621 cctggccgcc gaccacctgg tcctccagat tgcatccgac cgcgcccgtc tcggccagcc
     4681 gacggatatt ctcggcgccc acctccggct cggtcgcata cgcgccttcg aaatcgaccg
     4741 tcaccggaag gtcgaccgcg cccacgatcc gcgccgcatt ggcgagcacg agctcgagcg
     4801 gcagcatttc ggcatcgcca aagccgtgcg cctccgcgac cgaatggctg ccggtcgcga
     4861 tcgccttggc gcccccctcg gccaccgccc tggcgctgcc cgcgtcccag acgttgaaca
     4921 ggatcagcgg atcgcccggc cgatgcaacg cggcgaacag agcggctttc tggctcacgg
     4981 atcccatccc cacctcctca tcattccagc acaagctcgt cattccagcg aaagctgcct
     5041 cgtcattcca gcgcaagctg gaatctccct tcttcttggg ccggcc
//
LOCUS       DASVRK010000195         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_474617, whole genome shotgun sequence.
ACCESSION   DASVRK010000195 DASVRK010000000
VERSION     DASVRK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474617"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VF589_07645"
     CDS             complement(<1..572)
                     /locus_tag="VF589_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX8257491.1"
                     /translation="MVAHVATVAFLGLEARAVEVQVQIASGLPKFLVVGLPDKAVGES
                     RERVHAALASIGLSLPPKRITVNLSPADLPKEGSHYDLPIALGLLAAMGAIDTETLSE
                     YLAVGELGLDGRLTPSPGVLLAAMHASSRSLGLVCPAAQGSEASWAGDIEVVAAPDLI
                     ALLNHFRGSAALAPPAPGEAEPRGSGPDLT"
     gene            677..1006
                     /locus_tag="VF589_07650"
     CDS             677..1006
                     /locus_tag="VF589_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257492.1"
                     /translation="MAAMKLRERYVALGRVPAVRTCLFGLGLVLMMSGPLVGLLPGPG
                     GTIVFGAGLALALKYSEWAKRRYVRFKRRHPNKGRWADWGMRRRSAKRREALRKLRKK
                     AEALLDD"
     gene            1095..1229
                     /gene="rpmH"
                     /locus_tag="VF589_07655"
     CDS             1095..1229
                     /gene="rpmH"
                     /locus_tag="VF589_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX8257493.1"
                     /translation="MKRTFQPSNLVRKRRHGFRARKATVGGRKILAARRARGRKTLSA
                     "
     gene            1286..1552
                     /gene="rnpA"
                     /locus_tag="VF589_07660"
     CDS             1286..1552
                     /gene="rnpA"
                     /locus_tag="VF589_07660"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX8257494.1"
                     /translation="MPGFVLLVRGREDGDPAMRLGITVTKKIGGAVVRNRMKRRFRSL
                     AREILPEAGFAGADHVLVGRAGGVERPFAQLREELARALAKLRR"
     gene            1549..1761
                     /gene="yidD"
                     /locus_tag="VF589_07665"
     CDS             1549..1761
                     /gene="yidD"
                     /locus_tag="VF589_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX8257495.1"
                     /translation="MIAQLLILMAKGWQKGPSRILPSSCRYMPSCSAYAIGAIERYGA
                     LKGAWLAGRRLSRCHPWGGSGYDPVP"
     gene            1786..>2286
                     /gene="yidC"
                     /locus_tag="VF589_07670"
     CDS             1786..>2286
                     /gene="yidC"
                     /locus_tag="VF589_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX8257496.1"
                     /translation="MSDNRNMILAIALSALVLFGWSFLSDRFFPTANPPSTKMVDGKQ
                     VPLPKPLADPAADAPSAIRDRRQVLAETPRVRIESPSLRGSVNLRGAKIDDLVLTRHR
                     ENIADNSPPIRLFSPSGAPDAYYAGFGWTGEGLTLPGPDTQWTATGNRLAPGSPVTLS
                     WNNGQGQ"
BASE COUNT          344 a          795 c          805 g          342 t
ORIGIN      
        1 tgggtgagat cgggcccgga cccgcgcggc tccgcctcgc ccggcgcggg cggcgccagc
       61 gccgccgagc cgcggaaatg gttgagcaag gcgatgaggt cgggcgcggc gaccacctcg
      121 atgtcgccgg cccagctcgc ctccgaccct tgcgcggccg ggcagaccag gccgaggctg
      181 cgcgaggaag cgtgcatcgc cgccagcagc acgcccggcg agggcgtcag ccggccgtcg
      241 agcccgagtt cgccgaccgc cagatattcg gacagcgtct cggtgtcgat cgcgcccatt
      301 gccgccagca ggccgagcgc gatcggcagg tcgtaatgcg acccctcctt gggaaggtcg
      361 gccggcgaga ggttgaccgt gatccgcttg ggcggcagcg acaggccgat cgacgcgagg
      421 gccgcgtgga cccgctcccg gctttcgccg accgctttgt ccggcagacc gacgacgagg
      481 aacttgggaa ggccgctggc gatctgcacc tgcacttcga cggcgcgggc ctcgagcccc
      541 aaaaaagcta ccgtggcgac gtgcgcgacc atccagcccc tccaaaacgg actccatagt
      601 ggagtgactc cgcgtttgtg tcgatagccg ccaagcggac cgggccgtcc ttgagccgcg
      661 ccccgtcgcc ccccacatgg ccgcgatgaa gctgcgcgag cgatatgtcg ccctcggacg
      721 ggtgccggcg gtaaggacct gcctgttcgg gctcgggctc gtgctgatga tgagcgggcc
      781 tttggtcggc ctgctacccg gtcccggcgg cacgatcgtg ttcggcgccg ggcttgccct
      841 cgccctcaaa tatagcgaat gggcgaagcg gcgttatgtg cggttcaagc ggcggcaccc
      901 gaacaagggc cgctgggccg attgggggat gcggcggcgc agcgccaagc ggcgggaagc
      961 gctccgcaag ctgcgcaaga aggcggaggc cctgcttgac gattgacttt gcgggaaggc
     1021 ttgcctatcc gcgcctgcaa cgaggcggcc ccctttttcg gccgcccttt tcattagttt
     1081 tcgaggtatg agcgatgaag cgcaccttcc agccgagcaa tctcgtgcgc aagcggcggc
     1141 acggcttccg ggcccgcaag gcgaccgtgg gcggccgcaa gatcctggcg gcgcggcgcg
     1201 cccgcggccg caagacgctg tcagcctaac tacgatcagc cgccgggccg actttctcgc
     1261 ggccaatcgc ggcaagcgcg cgccgatgcc cggcttcgtg cttttggtgc gcggccggga
     1321 ggatggcgat cccgccatgc ggctcggcat caccgtcacc aagaagatcg gcggcgcggt
     1381 cgtgcgcaac cgcatgaagc ggcgcttccg ctcgctggcg cgggagatcc tgccggaggc
     1441 cggtttcgcg ggcgccgacc atgtcctcgt cggccgggcc ggcggggtcg agcggccgtt
     1501 cgcgcagctt cgcgaggagc ttgcgcgggc gctggcgaag ctccggcgat gatcgcgcag
     1561 ctgctcatcc tgatggccaa gggctggcag aagggtccgt cgcggatcct gccctcctcg
     1621 tgccgctaca tgccgagctg ctcggcctat gcaatcggcg cgatcgagcg ctatggggcg
     1681 ctgaagggcg cctggctcgc gggacggcgc ctgtcgcgat gccatccctg ggggggatca
     1741 ggctatgacc cggtaccgtg aaggccgaag cgtgaaggga agtcagtgag cgacaaccgc
     1801 aacatgatcc tggcgatcgc cctgtcggcg ctcgtgctgt tcggctggag cttcctctcc
     1861 gaccgcttct tcccgaccgc caacccgcct tcgaccaaga tggtcgacgg caagcaggtc
     1921 ccactgccca agccgctcgc cgatcccgcg gccgacgctc cgtcggcgat ccgggaccgc
     1981 cgccaggtgc tcgccgaaac gccgcgcgtg cggatcgaat cgccaagcct ccggggctcg
     2041 gtcaacctgc gcggcgccaa gatcgacgat ctggtgctca cccgccaccg cgagaacatc
     2101 gccgacaatt cgccgccgat ccggctgttc tcgccgagcg gcgcccccga cgcttattat
     2161 gcgggcttcg gctggaccgg tgagggcctg accctgccgg ggccggatac ccaatggacc
     2221 gccaccggca atcggctggc gccgggcagc ccggttacgc tgagctggaa taacggccag
     2281 ggccag
//
LOCUS       DASVRK010000196         7182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_474733, whole genome shotgun sequence.
ACCESSION   DASVRK010000196 DASVRK010000000
VERSION     DASVRK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474733"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VF589_07675"
     CDS             <1..558
                     /locus_tag="VF589_07675"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8257497.1"
                     /translation="LIGSTSGDDLTGHGGANRIDGRGGDDSLSGLQGDDELIGQGGHD
                     RLSGGDGFDIIVGGTGNDTIIGGGHRDELTGSAGADSFVFFPGQLTDARGEADVITDF
                     AATGGDLINLRLVDAKDSTPESDDAFTFIGTSAFSGNGVGGELRYQIVEGNTLVSGDI
                     DGDGTAEFYIRLNGPVELTASDFVL"
     gene            complement(606..848)
                     /locus_tag="VF589_07680"
     CDS             complement(606..848)
                     /locus_tag="VF589_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEX8257498.1"
                     /translation="MDRPSWRKPAGVGLILLLILVWGAIVATAADWLDGLPWPVHALY
                     YTVAGIVWILPLKPLLRWMETGRWRAPADAAGPGRQ"
     gene            complement(841..1392)
                     /locus_tag="VF589_07685"
     CDS             complement(841..1392)
                     /locus_tag="VF589_07685"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX8257499.1"
                     /translation="MRALALERRRSYARSLGPGLREDLEAKLARIVLPRLVGARVVAG
                     YHPMKDEISPYALLDRLGAGQRAALPWFADRDARMMFRAAPAVEPGPWGVLQPPADAE
                     ALAPDLVLVPLVLGDRRGNRIGHGKGHYDRALGHLRDGGNAVSTIGIAWETQISEEPI
                     PADPWDVPLGAIATPAEWIDCHG"
     gene            complement(1580..1900)
                     /locus_tag="VF589_07690"
     CDS             complement(1580..1900)
                     /locus_tag="VF589_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX8257500.1"
                     /translation="MASIDVEIASRRYAVACRDGEEPHLKSVAAIVDRKARDAAAALG
                     TLSESRQLLFASLLLADELKERREGSGSPAAEPDPGLAEALERLADRIESLAERLEAA
                     ASSP"
     gene            complement(1902..2108)
                     /locus_tag="VF589_07695"
     CDS             complement(1902..2108)
                     /locus_tag="VF589_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257501.1"
                     /translation="MADEQAMQAVSRIERALARIEAAAAKERPAPREDGELRELRRMH
                     QSLRGTVEDAIGQIDRLLAVREGE"
     gene            2267..4246
                     /gene="tkt"
                     /locus_tag="VF589_07700"
     CDS             2267..4246
                     /gene="tkt"
                     /locus_tag="VF589_07700"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX8257502.1"
                     /translation="MPAEPRLLANAIRALSMDAVEAANSGHPGMPMGMADAATILFTE
                     YLKYDPQDPGWHDRDRFVLSAGHGSMLIYSLLHLTGYAHPTMDEIRRFRQLGSPCAGH
                     PENFMMKGIEATTGPLGQGFAMAVGMAIAERHLNAVFGDQLVDHRTWIISGDGDLMEG
                     INHEAAGLAGHLQLGRLNVLWDNNKITIDGATDLSTSEDVQARYRASGWHVVECDGHD
                     FDSIRRALDEAVADPRPALIDCRTIIGWGAPNKMGTAATHGAALGLEEVKAARAHLVW
                     EHEPFELPDEIVDTWRAAGRRSADAHNAWRARLDGHGERDEFLRRMEGRLPADFSLDA
                     YLAGLAAEPKKVATRKASELALEAVNAALPETVGGSADLTGSNNTKTKTQKPLTRDDY
                     SGRYIYYGIREFGMAAAMNGMALHGGVIPYGGTFLVFSDYCRPAIRLSALQGARVVYV
                     MTHDSIGLGEDGPTHQPVEHVMSLRMIPNLAVYRPADAVETAECWALALERGDGPSLL
                     ALTRQNLPQVRLERSENMSARGAYRLREAQAERQVVLIATGSEVEVALAAADALEAQG
                     IGADVVSMPCWSLFDAQDGAYRAGLLPADSLKVSVEAGTTLGWERYIGPRGLAIGLDR
                     FGASAPAEDLFKRFGFTVDAIVPRILAALQKQGDQ"
     gene            4248..5261
                     /gene="gap"
                     /locus_tag="VF589_07705"
     CDS             4248..5261
                     /gene="gap"
                     /locus_tag="VF589_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX8257503.1"
                     /translation="MAVRVAINGFGRIGRLVARAILEKADSGLELVAVNDLADAKSNA
                     MLFKRDSVHGRYPGEVSADGNDLIIDGRRIKVTAERDPGKLPHGDNGVDIALECTGFF
                     ADRDKAGAHLTAGAKRVLISAPAKGVDLTVVYGVNHDKLTAEHTIVSNASCTTNCLAP
                     VAKVMNDVIGIERGLMTTIHAYTNDQKILDQIHPDMRRARAAGMSMIPTTTGAARAVG
                     EVLPELKGKLDGSAVRVPTPNVSLVDLTFTPKRDTSRDEVNGALRAASESGPLKGILA
                     YTDEPLVSIDLNHNPASSTVDSLETAVLEGKLVRVVSWYDNEWGFSNRMVDTAGAMAR
                     LIG"
     gene            5341..6552
                     /locus_tag="VF589_07710"
     CDS             5341..6552
                     /locus_tag="VF589_07710"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX8257504.1"
                     /translation="MAGFNTLDDVGDVAGKRVLVRVDLNVPMQDGRVSDDTRLRATLP
                     TVMELADKGAIVLLLAHFGRPKGQPAAEYSLAQVREAYEAVLGRPVRYVDWEGVDGAV
                     AALRGGEVAILENTRFFGGEEKNDPAVADRFAGLGDLYVNDAFSAAHRAHASTEGLAH
                     RLPAFAGRAMEAELKALEKALGAPERPVAAVVGGAKVSTKLEVLHHLVAKVDHLIIGG
                     GMANTFLAARGVGVGKSLAEHDLTGTALDILDAAERANCTVHLPYDVVVAKEFAPNPP
                     SVRTCNVHEVAADEMILDVGPAAVEALADALKTCRTLVWNGPLGAFETPPFDRATVAL
                     ARTAAALTQSGSLVSVAGGGDTVAALNHAGVAGDFTFVSTAGGAFLEWMEGKALPGVA
                     ALQLQHPQDHP"
     gene            6586..>7182
                     /locus_tag="VF589_07715"
     CDS             6586..>7182
                     /locus_tag="VF589_07715"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX8257505.1"
                     /translation="MMTQIAEGKGFIAALDQSGGSTPKALKGYGVEEDSWTSEKEMFG
                     LIHQMRCRIITSPVFTGDKVIGAILFERTMDGEAEGKPVPQLLKESGVVPFIKIDKGL
                     EAEENGVQLMKPMPELGALLARAKRLGVFGTKERSVINLANREGIAAIVRQQIEVGRE
                     VLAAGLIPILEPEINIKSPERAGADRILLEELTRALDAM"
BASE COUNT         1191 a         2466 c         2436 g         1089 t
ORIGIN      
        1 ctgatcggat cgacttccgg cgacgatctg acgggccatg gcggggctaa ccggatcgac
       61 ggccgcggcg gcgacgattc gctcagcggg ctgcagggcg atgacgagct catcggccag
      121 ggcgggcacg accggctgtc cggcggcgac gggttcgaca taatcgtggg aggcaccggc
      181 aacgatacga tcatcggggg cgggcaccgc gacgagctga ccggtagcgc gggcgccgac
      241 tcgttcgtct tcttccccgg ccaactgacc gacgcgcgcg gggaagcgga tgtgatcacg
      301 gacttcgccg cgaccggcgg cgatctcatc aatctgcgcc tcgtcgacgc caaggacagc
      361 acgcccgaga gcgacgacgc gttcaccttc atcggcacgt ccgccttttc gggaaacggc
      421 gtcggcggcg agctgcgcta tcagatcgtc gagggcaaca ccttagtgag cggcgacatt
      481 gacggcgacg gcaccgccga attctacatc cggctcaacg gcccggtcga gctcaccgcg
      541 tcggatttcg tgctctagcc ggagcgacaa gctaggcttg cggggggggc tcggtcccgc
      601 ccgcctcact ggcggccggg gccggctgcg tccgccggcg cgcgccagcg gcccgtctcc
      661 atccagcgca gcagcggctt cagtggcagg atccagacga tcccggcgac cgtgtagtaa
      721 agcgcgtgga ccggccaggg caggccgtcg agccagtccg cagcggtggc gacgatcgcg
      781 ccccagacca ggatcagcag caggatgagg ccgaccccgg ccggtttgcg ccagctcggt
      841 ctatccatgg cagtcgatcc attcggccgg ggtggcgatc gcgccgagcg gcacgtccca
      901 ggggtcggcg gggatcggct cctcgctgat ctgcgtctcc caggcgatgc cgatggtgga
      961 gacggcattg ccgccgtcgc gcaggtgccc cagcgcgcgg tcgtaatggc ccttgccgtg
     1021 gccgatccgg ttgccgcgcc ggtcgccgag caccagaggc accagcacca ggtccggcgc
     1081 cagcgcttcg gcgtcggccg gcggctggag cacgccccac gggccgggtt cgaccgcggg
     1141 cgcggcgcgg aacatcatcc gcgcgtcgcg atcggcgaac caggggaggg cggcgcgctg
     1201 ccccgccccg agccggtcga gcaatgcata ggggctgatc tcgtccttca tcggatgata
     1261 gccggcgacg acccgcgcgc cgaccaggcg cggcagcacg atccgggcga gcttcgcttc
     1321 gagatcttcg cgcagcccgg ggcccagcga gcgcgcgtag ctgcgccgcc gctcgagagc
     1381 gagagcgcgc atctgcgcct ttggaggggg tgacgggacc gccatggccg tttgccggaa
     1441 tatcctctga cgccagtacg tcaggtgggg accatgtgct tcggaccagg atcctagcag
     1501 ggacagcccc cgaggatgtc ttatcgcctc agggatgttc acatacgctc gtaccgggca
     1561 gtacccgtca agctcctatc tagggactcg aagccgccgc ctcaagcctt tccgccaggc
     1621 tctcgatacg gtcggcgagc cgctccagcg cctcggccag gcccgggtcc ggttcggccg
     1681 ccggggagcc cgagccttcg cggcgctcct tgagctcgtc ggcgagcagc aaggaggcga
     1741 acaacaattg gcgcgattcg ctcaatgtgc cgagcgcggc ggcggcatcg cgcgccttgc
     1801 ggtcgacgat cgcggccacc gatttgaggt gcggctcctc gccgtcgcgg caggcgaccg
     1861 catagcggcg ggacgcgatc tcgacatcga tcgaagccat attattcccc ctcgcggacg
     1921 gccagcagcc ggtcgatctg gccgatcgca tcctcgaccg tgccgcgcag cgactggtgc
     1981 atccggcgga gctcgcgcag ctcgccatcc tcgcgcggcg ccggccgctc ttttgccgcg
     2041 gcggcctcga tccgggccag cgcccgctcg atccggctca ccgcctgcat cgcctgttcg
     2101 tctgccatat gaacccgaat aaccgtgcgc gcacatggcg gcaagcgatt gaggcgatgg
     2161 ggcgcggttg accttggcgg ctccacgctt aaagggagcg ggcggtggga gcgcccgagt
     2221 ttccgccgcc gtcccgtccg ggacattccg agcttaaggg aatccgatgc ccgccgaacc
     2281 gagattgctc gccaatgcca tacgcgcgtt gtcgatggac gcggtcgagg cggctaatag
     2341 cggccatccc ggaatgccga tgggaatggc cgacgcggcc acgatcctgt tcacggaata
     2401 tctgaagtac gatccgcaag accctggttg gcatgatcgc gaccggttcg tgctttcggc
     2461 cggccatggc tcgatgctga tctattcgct gctccatctg accggctacg cccatccgac
     2521 catggacgag atccgccgct tccggcagct cggcagcccg tgcgcggggc acccggaaaa
     2581 cttcatgatg aaagggatcg aggccaccac cggcccgctc ggccagggtt tcgcgatggc
     2641 cgtcgggatg gcgatcgccg agcgccacct gaacgcggtg ttcggcgacc aactggtcga
     2701 ccaccgcacc tggatcatat cgggcgacgg cgacctgatg gaggggatca accacgaggc
     2761 ggccggactt gccggccatt tacagcttgg ccgccttaac gtcctgtggg acaacaacaa
     2821 gatcaccatc gacggcgcga ccgatctgtc gacctcggag gacgtccagg cccgctaccg
     2881 cgcctcgggc tggcatgtgg tcgagtgcga cggccacgat ttcgacagca tccgccgcgc
     2941 gctcgacgaa gccgtcgccg atccgcggcc ggcgctgatc gattgccgga ccatcatcgg
     3001 ctggggcgcg cccaacaaga tgggcaccgc cgccacccac ggtgccgcgc tcgggctcga
     3061 ggaagtgaag gcggcgcgcg ctcatctcgt ctgggagcat gagccgttcg agcttcccga
     3121 cgagatcgtc gacacctggc gcgcggccgg ccggcgcagc gcggacgcgc acaacgcgtg
     3181 gcgcgcgcgg ctcgacggcc atggcgagcg ggacgagttc ctgcgccgga tggagggaag
     3241 gctgccggcg gacttctcgc tcgacgccta tctggcgggg ctcgccgccg agccgaagaa
     3301 ggtcgcgacc cgcaaggcgt ccgaactcgc gctcgaggcg gtcaatgcgg cgctgcccga
     3361 gacggtcggc ggctccgccg acctcaccgg ttcgaacaac accaaaacca agacgcagaa
     3421 gccgctgacc agggacgatt attccgggcg ctacatctat tacggcattc gcgaattcgg
     3481 catggccgcg gcgatgaacg ggatggcgct gcacggcggc gtaatccctt atggcggcac
     3541 cttcctggtc ttttccgatt attgccggcc ggcgatccgg ctgtcggcgc tgcagggggc
     3601 gcgggtcgtc tatgtgatga cgcacgattc gatcggcctc ggcgaggatg ggcccaccca
     3661 tcagccggtc gagcatgtga tgagcctgcg gatgatcccc aacctcgccg tctaccggcc
     3721 cgccgacgcg gtcgagacgg ccgaatgctg ggcgctggcg ctcgaacgcg gcgatgggcc
     3781 gtcgctgctt gcgctgaccc gccagaacct gccgcaggtc cggcttgagc ggagcgagaa
     3841 tatgtcggcc cgcggcgcct accgccttcg cgaggcgcaa gccgagcggc aagtggtgct
     3901 gatcgcgacc ggctcggaag tcgaagtcgc gctcgccgcg gccgatgcgc tcgaggcgca
     3961 ggggatcgga gccgacgtgg tttcaatgcc ttgctggtcg ttgttcgatg cccaggacgg
     4021 ggcctaccgc gccggcctgc tgcccgcgga cagcctcaaa gtgtcggtcg aggcggggac
     4081 gacgctcggc tgggaacgct atatcgggcc gcgcggcctc gctatcggcc ttgaccggtt
     4141 cggcgcctcc gcccccgcgg aagatctgtt caagcgcttc ggctttaccg tcgacgcgat
     4201 cgtgccccgc atattggcgg cgcttcaaaa gcaaggagat caatgatatg gccgtcagag
     4261 ttgcgatcaa tggtttcgga cgtatcggcc ggctggtggc gcgcgccatc ctcgaaaagg
     4321 ccgatagcgg gctcgagctg gtcgccgtca acgacctcgc cgacgccaaa tccaacgcca
     4381 tgctgttcaa gcgcgactcg gtccatggcc gctatccggg cgaggtgtcg gccgacggca
     4441 acgacctcat catcgacggc cgccgcatca aggtcaccgc cgagcgggat ccgggtaagc
     4501 tgccgcacgg cgacaatggc gtcgacatcg cgctcgaatg caccggcttc ttcgccgacc
     4561 gcgacaaggc cggcgcgcac ctcaccgccg gcgccaagcg cgtcctcatc tccgcgccgg
     4621 ccaagggcgt cgacctcacc gtcgtctacg gcgtcaacca cgacaagctg accgccgagc
     4681 acaccatcgt ttccaacgcg tcctgcacca ccaattgcct cgcaccggtt gcgaaggtca
     4741 tgaacgatgt aattggcatc gaacgtggat tgatgaccac catccacgct tataccaacg
     4801 atcagaagat cctcgaccag atccacccgg acatgcgccg cgcccgcgcc gccggcatgt
     4861 cgatgatccc gaccaccacc ggcgccgccc gcgcggtcgg cgaggtgctg cccgaactca
     4921 agggcaagct cgacggctcg gcggtgcggg tgccgacgcc gaacgtcagc ctggtcgacc
     4981 tcaccttcac gcccaagcgc gacaccagcc gcgacgaagt gaacggcgcg ctgcgggcag
     5041 cctcggagag cgggccgttg aagggcatcc tggcttatac cgacgagcct ctggtctcga
     5101 tcgacctcaa ccacaatccg gcgagctcga ccgtcgacag cctcgagacc gcggtgctcg
     5161 aaggcaagct ggtccgggtc gtctcctggt acgacaatga atggggcttc tcgaaccgga
     5221 tggtcgacac cgccggcgcg atggcgcggc tgatcggctg acacgcggcc tcgatgaaca
     5281 tgagccgctc atgcttcgcc cggcccggcg gcacataggg tgagcgggca gggagcaaag
     5341 atggccggtt tcaacacctt ggacgacgtc ggcgatgtcg cgggcaagcg cgtgctcgtg
     5401 cgcgtcgacc tcaacgtgcc gatgcaggac ggccgggtca gcgacgacac gcggctgcgc
     5461 gccactttgc cgacggtgat ggagcttgcc gacaaaggcg cgatcgtcct cctgctcgcc
     5521 catttcggtc gacccaaggg gcagccggcg gctgaatatt cgctcgcgca ggtgcgggag
     5581 gcctatgagg cggtgctcgg gcggccggtg cgctacgtcg actgggaagg cgtcgacggg
     5641 gcggtggccg cgctccgggg cggcgaggtg gcgatcctcg aaaatacccg cttcttcggc
     5701 ggcgaggaga agaacgatcc cgccgtcgcc gatcgcttcg ccggcctcgg cgacctctac
     5761 gtcaacgatg ccttttccgc cgcccaccgc gcccacgcct cgaccgaggg gctcgcccac
     5821 cggctgcctg cttttgccgg ccgggcgatg gaggcggagc tgaaggcgct cgaaaaggcg
     5881 ctcggcgcgc ccgagcggcc ggtcgccgcg gtggtcggcg gcgccaaggt ctcgaccaag
     5941 ctcgaagtgc tccaccactt ggttgccaag gtcgaccacc taatcatcgg cggcggcatg
     6001 gccaacacct tcctcgccgc gcgcggcgtc ggggtcggca agtcgctcgc cgagcacgac
     6061 ctcaccggca ccgcgctcga catattggac gccgccgagc gggccaattg caccgtccac
     6121 cttccctatg acgtcgtggt ggcgaaggaa ttcgctccga acccgccgtc ggtccgcacc
     6181 tgcaacgtcc atgaagtcgc cgcggacgag atgatcctcg acgtcggccc ggctgcggtc
     6241 gaggcgctgg ccgacgcgct caagacctgc cgcacgctcg tgtggaacgg cccgctcggc
     6301 gcgttcgaga cgccgccctt cgaccgcgcc acggtggcgc tcgcccgcac cgccgccgcg
     6361 ctgacgcaga gcggctcgct ggtctcggtc gccggcggcg gcgacacggt cgcggccctc
     6421 aaccatgccg gggtggccgg cgatttcacg ttcgtctcga cagcgggcgg cgccttcctt
     6481 gaatggatgg aaggcaaggc attgccgggc gtcgcggcgc tgcagcttca gcaccctcaa
     6541 gatcacccat agccgctaag gatcgactta atgcaagata acgagatgat gacgcaaatc
     6601 gctgaaggca aagggttcat cgccgcgctc gaccagagcg gcggttcgac ccccaaggcg
     6661 ctcaagggct atggcgtcga ggaagatagc tggaccagcg agaaggagat gttcgggctg
     6721 atccaccaga tgcgctgccg catcatcacc tcgcccgtct tcaccggcga caaagtgatc
     6781 ggggcgatcc tgttcgagcg gaccatggac ggcgaggccg agggcaagcc ggtgccgcag
     6841 ctgctcaagg agagcggcgt cgtcccgttc atcaagatcg acaagggcct ggaggccgag
     6901 gagaatggcg ttcagctgat gaagccgatg ccggagctcg gcgccttgct cgcccgcgcc
     6961 aagcgcctcg gcgtgttcgg caccaaggag cggtcggtga tcaacctcgc caaccgcgaa
     7021 ggcatcgccg ccatcgtccg ccagcagatc gaggtcgggc gcgaagtgct cgctgccggc
     7081 cttatcccga tcctcgagcc cgaaatcaac atcaagagcc ccgagcgcgc cggggccgac
     7141 cgcatcctgc tcgaggagct cacccgcgcg ctcgatgcga tg
//
LOCUS       DASVRK010000197         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_225758, whole genome shotgun sequence.
ACCESSION   DASVRK010000197 DASVRK010000000
VERSION     DASVRK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225758"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VF589_07720"
     CDS             <1..809
                     /locus_tag="VF589_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8257506.1"
                     /translation="LARAGAKVFVNDIPQSLANAEQVVEEIRSAGGQAEAVCASVSDE
                     AAVAEMVGQVGTVDILVNNAGILRDRSFAKMSMADFRAVVDVHLIGSAHCTKAVWDGM
                     REQNYGRILMTSSSTGLFGNFGQANYGAAKLGLLGLARTLHLEGARYNVRVNTIAPTA
                     ATRMTEDIFPPAMLGAFDPANVVPAALFLVSEDAPSGAIVGAGAGVVQACYVTLTPGV
                     ALPEGRRTLEEVAANWERIASRDGEIVPQSGAEQALLILKTLQGEAGYSG"
     gene            1284..>2577
                     /locus_tag="VF589_07725"
     CDS             1284..>2577
                     /locus_tag="VF589_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257507.1"
                     /translation="MANFPTEGDDVLNGTAESDLIEALGGNDVINGRGGADTMRGGSG
                     NDTYYVDHVGDLAEEVAEDGDGTDNVRSMVSFVLGDFVENLTLLGIGDINGTGNGDAN
                     VIIGNAGRNRLTGGGGIDTMKGGKGNDTYVIGLGDQIFETADGGTADRVQSALSYTLR
                     ANFENLTLTGAAHISGNGNEDANLIIGNNGNNRLDGRGGADTLQGGLGNDIYVLGLGD
                     TASEAAGAGIDTVQTALTHILGANIENLTLTGTAAVNGTGNDLANVIVGNSGANRLSG
                     GLESGGAQADSLKGGNGDDVYVVGAGDKVFETSALGGVDMVESSVSYRLGANLEKLDL
                     TGSAAINGIGNSLANMLFGNGAANVLTGEAGADYIDGGSGADTMRGGTENDIYVVDSV
                     SDVVEENANEGFDTIGTLLTSMTLNIANVEGLVYVGNGS"
BASE COUNT          472 a          901 c          823 g          381 t
ORIGIN      
        1 tgctggcgcg cgcgggcgcc aaggtgttcg tcaacgacat cccccagagc ctcgccaatg
       61 ccgagcaggt ggtcgaagag attcgcagcg ctggcggcca ggccgaagcg gtctgcgcgt
      121 ccgtctccga cgaagccgcc gtcgccgaga tggtcggcca ggtcggcacc gtcgacatcc
      181 tcgtcaacaa tgccggcatc ctgcgcgacc gcagcttcgc caagatgtcc atggcggact
      241 tccgtgcggt ggtggacgtg cacctcatcg gctcggccca ttgcaccaag gccgtctggg
      301 acggcatgcg cgagcagaat tacggccgca tcctgatgac ctcctcctcg accggcctgt
      361 tcggcaattt cggccaggcc aattacggcg ccgccaagct cggcctgctc ggcctcgccc
      421 gcacgctcca ccttgaagga gccagataca acgtccgcgt caacacgatc gcgccgaccg
      481 cggcgacgcg gatgaccgag gacatcttcc cgccggcgat gctcggagcg ttcgaccccg
      541 ccaatgtcgt cccggcggct ctgttcctcg tctccgaaga cgccccgtcc ggcgcgatcg
      601 tcggcgccgg cgccggcgtc gtccaggcct gctacgtcac gctgacgccc ggcgtggcgc
      661 tgccggaggg ccggcgcacc ctcgaagagg tcgccgccaa ctgggagcgc atcgccagcc
      721 gcgacggcga gatcgtcccg caatccggcg ccgagcaggc gctgctgatc ctgaagaccc
      781 tgcagggcga agcgggatat agcggctgaa gcggggatag ggcggcgagc cgaggtagga
      841 atcacctctt gccgctcatc ctgaggagcc ggtgagcccg tcgaaccggc gtctcgaagg
      901 acgcaccttg tccttcgaga cgggccttcc cccggatcag gtccggggtc agtccctcct
      961 caggatgagc gggttagcct aagacgagcc cccgaccccg cgtcccgttt cgaaccaccc
     1021 cgtctttcgc ggaacccgct cgacttgccg ccgccaagtc gaggagagcc cgcaccttgc
     1081 ggccgattag tcaatttgca gatgatcagc cgatccgatc atgttaaatg agcggcttac
     1141 cggagtcggc cggatatcgg ccgacgctcc tcgggggggc gcctgttcca gcgccggtgt
     1201 gagtctttcg accgacggcg ggcgcggccc gcgcgatcgg tttcgggccg ttcgcgccca
     1261 aagctatggg ggggagacga tcgatggcga attttccgac tgaaggcgac gacgtactca
     1321 acggcaccgc cgagtccgac ctgatcgagg cgcttggcgg caacgacgtc atcaacggcc
     1381 gcggcggcgc cgacaccatg cgcggcggca gcggcaacga cacttattat gtcgaccatg
     1441 tcggcgacct cgccgaggaa gtcgccgagg atggcgacgg caccgacaat gtcaggagca
     1501 tggtgagctt cgtgctcggc gatttcgtcg agaatctgac gctgctcggc attggcgaca
     1561 tcaacggcac cggcaatggc gatgccaacg tcatcatcgg caatgccggc cgcaaccgcc
     1621 tgaccggcgg cggcggcatc gacacgatga agggcggcaa gggcaacgac acttatgtga
     1681 tcggcctcgg cgaccagatt ttcgagaccg ccgacggcgg caccgccgac cgcgtgcaga
     1741 gcgcgctcag ctacacgctg cgcgccaatt tcgagaacct cacgctcacc ggcgccgccc
     1801 acatcagcgg caacggcaat gaggacgcca acctcatcat cggcaataac ggcaacaacc
     1861 ggctcgacgg ccgcggcggc gccgacacgc ttcagggcgg cctcggcaac gacatctacg
     1921 tgctcggcct cggcgacacc gccagcgagg ccgccggtgc cgggatcgac accgtccaga
     1981 ccgccctcac ccacattctc ggcgccaata tcgagaatct gaccctcacc ggcaccgccg
     2041 ccgtcaacgg caccggcaac gacctcgcca acgtcatcgt cggcaatagc ggcgccaacc
     2101 ggctcagcgg cgggctcgag agcggcggcg cccaggccga ctcgctgaag ggcggcaatg
     2161 gcgacgacgt ctatgtggtc ggcgcgggcg acaaggtgtt cgagaccagc gcgctcggcg
     2221 gcgtcgacat ggtcgaaagc tcggtcagct accggctcgg cgccaatctc gagaaactcg
     2281 acctcaccgg cagcgccgcc atcaacggca tcggcaattc cctcgccaac atgctgttcg
     2341 gcaacggcgc cgccaacgtc ctcaccggcg aggcgggcgc cgactatatc gacggcggca
     2401 gcggcgccga caccatgcgc ggcggcaccg agaacgacat ctacgtcgtc gatagcgtat
     2461 ccgacgtggt cgaagagaat gccaatgaag gcttcgacac gatcggcact ttgctcacgt
     2521 ccatgactct caacatcgcc aatgtcgaag gcctggtcta tgtcggcaac ggcagct
//
LOCUS       DASVRK010000198         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_536385, whole genome shotgun sequence.
ACCESSION   DASVRK010000198 DASVRK010000000
VERSION     DASVRK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536385"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..918)
                     /locus_tag="VF589_07730"
     CDS             complement(61..918)
                     /locus_tag="VF589_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8257508.1"
                     /translation="MGFDRGRRGERGGRGRDKRDGFGDENFSGFDDRGGGGFGGGGSG
                     GGYRGGGGGDRFGGGGGGGYGGGGGGGYRGGGGGGFGGGGGGGGFRGGGGGGGGGMPP
                     QVVGQGKGVVKFFNAQKGFGFVVRDDGGEDVFVHISAVEQAGLTGLADGQPLEFTLVD
                     RGGRISATDLKIDGEPMPVSDRAPPREPREGGPGGGGYGGAPGGDRGGPQRQLTGEKA
                     QGTVKFFNAMKGFGFISRDDGQPDAFVHISAVERAGMSSLNEGDRVEFELEVDRRGKT
                     AAVNLSSLG"
     gene            complement(1083..2015)
                     /locus_tag="VF589_07735"
     CDS             complement(1083..2015)
                     /locus_tag="VF589_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEX8257509.1"
                     /translation="MPSGLAALLDDVAAIAKLAAASIDDVGAAAGRAGAKAAGVVIDD
                     TAVTPRYVTGFTPDRELPIIARIAKGSLKNKLLILLPAALALSAFLPWAITPLLMLGA
                     AYLCFEGAEKVWEAVSEPKHSLAEDAAELSSADHEQKMVSGAVRTDFILSAEIMAIAL
                     NEVADENIWTQGMILAVVAVAITVGVYGLVGLIVKMDDIGLHLTERRNRGSQALGRGL
                     VHAMPKVMAALSTIGIAAMIWVGGGIIVHGLEEFGVAGPGHLIHDVAAAAGHAVPALH
                     AAVEWVVAAAGAGLVGLVVGAPIVFVHHKVARKH"
     gene            2232..2849
                     /locus_tag="VF589_07740"
     CDS             2232..2849
                     /locus_tag="VF589_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8257510.1"
                     /translation="MTVHFHEEDLPEGVLAPGPVAVDTEAMGLLPGRDRLCLVQISDG
                     RGDEHLVRFNPGSDYGAPNLKAVLADPERLKLYHFARFDLAIMQAYLGVMAGPAYCTK
                     TASRLIRTYTDRHGLKELIKELLQIDISKQQQTSDWGAPELSDAQKDYAASDVRYLHQ
                     MKEKLDERLVREGRMPLARACFDFLPHRALLDLAGWAEVDIFAHV"
     gene            2842..>3467
                     /gene="lptC"
                     /locus_tag="VF589_07745"
     CDS             2842..>3467
                     /gene="lptC"
                     /locus_tag="VF589_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX8257511.1"
                     /translation="MSDLAEDRRRAKRGWAAPGGMHDWLMRFLKVALPAGVGLLLAYL
                     LLSPLSKEKEISFLLDKNKVDVARERLKTQKAEYRGLDDKGRAFSIQAAQAVQAKSSD
                     PVVDIGGMAARIQLQEGPATITADKARYNMDTQKVDVLGPILITAPDGYRLETSDVQV
                     DLNTHRLGSDAGVEGRMPLGRFSADQMRADLPSRKVVLSGRARLHIVQG"
BASE COUNT          561 a         1278 c         1097 g          531 t
ORIGIN      
        1 aaatgcccgg cgcgctctgg cgagcggccg ggctgattca ttccgccgaa gcgcggctgc
       61 ttagccgagc gagctcaggt tcaccgctgc ggttttgccg cggcggtcta cttccagctc
      121 gaactcgacc cggtcgccct cgttgaggct cgacatgccg gcacgctcaa ccgccgagat
      181 gtgcacaaac gcatccggct ggccgtcgtc gcggctgatg aagccgaagc ccttcatggc
      241 gttgaagaac ttcaccgtgc cctgggcctt ttcgccggtg agctggcgct gcgggccgcc
      301 gcggtcgccg ccgggcgcac cgccgtagcc gccgccgccc ggaccgcctt cgcgcggctc
      361 gcggggaggc gcacgatcgg aaaccggcat cggctcgccg tcgatcttga gatcggttgc
      421 cgaaatccgg ccgccgcgat cgacgagcgt gaactcgagc ggctggccat cggcaaggcc
      481 ggtaaggccc gcttgctcaa ccgccgagat gtgcacgaac acgtcttcgc caccatcgtc
      541 acggacaacg aagccgaagc ccttttgagc gttgaagaac ttaacgacgc ccttgccctg
      601 gccgacgacc tggggaggca tcccgccgcc accgccgccg ccgccgccgc ggaacccgcc
      661 gccgccgccg ccgccgccga agccgccacc gccgccgccg cggtaaccgc cgccgccgcc
      721 gccgccgtag ccgccaccgc caccgccgcc gaatcggtcg ccgccgccgc cgccacgata
      781 gccgccgccg gatccgccgc cgccgaagcc gccgccgccg cgatcatcaa atccgctgaa
      841 attctcgtcg ccgaaaccgt cgcgcttgtc tctgccgcgc ccgccgcgct caccgcggcg
      901 ccctctgtcg aaacccatgc ccgtctatac tttccgcgcc cctgccttca ctcgcaaacc
      961 accgcgcggg gctgtcgcgc aaacggtctc atgcgccccc gaaaatggcg caccgccgac
     1021 tcatataacc caaatcgtcc cgagccgcga gcgcttttcc acaggcgtct tcgagcgcga
     1081 gctcaatgct ttcgagcgac cttgtgatgg acgaagacta tcggagcgcc gacgacgagg
     1141 ccgacgaggc cggctccggc cgccgcgacc acccattcca ccgccgcgtg gagtgccgga
     1201 acggcgtgtc ccgccgcggc cgccacgtcg tggataaggt gcccgggacc ggcgactccg
     1261 aactcctcga gcccgtggac gatgatcccg cccccgaccc agatcatcgc cgcgatgccg
     1321 atggtggaga gtgctgccat caccttgggc atggcgtgga ccaggccgcg cccaagcgcc
     1381 tgcgagcccc ggttacggcg ctcggtgaga tgaaggccga tatcgtccat cttcacgatc
     1441 aggccgacga gcccgtagac tcccaccgta atcgccaccg cgaccaccgc caggatcatc
     1501 ccctgggtcc agatattctc gtcggccacc tcgttgagcg cgatcgccat gatctcggcc
     1561 gacaggatga aatcggtccg gaccgcgccg ctgaccatct tctgctcgtg atcggcgctg
     1621 ctcaattccg ccgcgtcctc ggcgaggctg tgcttcggct ccgacaccgc ctcccacacc
     1681 ttttcggcac cctcgaagca gagataggcc gcgcccagca tcagcagcgg cgtgatcgcc
     1741 cagggcagga aggcgctcaa cgccagcgcc gccggaagca ggatgaggag cttgttcttc
     1801 agcgatccct tggcgatccg agcgatgatc gggagctcgc ggtcgggtgt gaagccggtg
     1861 acgtagcgcg gcgtcaccgc cgtatcgtcg atcaccacgc ccgccgcctt ggcgcccgcc
     1921 cggccggcgg cggcgccgac gtcgtcgatc gaggcggcgg ccagcttggc gatggcggcg
     1981 acgtcgtcga ggagggcggc gagaccggac ggcatggcgg gagggctcct ggttgctcgc
     2041 gctgctgtgg cggggcgggg cgtcaggatc aagtgcgggc atcgaccgtc attccggcga
     2101 aggccggaat ccatgaccac cgtcgtcacc gactctccac gggccctgtt catggatccc
     2161 ggccttcgcc gggatgatga gctggcacga cggtcgttga gcgttaagct tcatcggcct
     2221 atgcgatagg catgacagtg catttccatg aagaggacct gcccgagggc gtccttgccc
     2281 ccggcccggt cgccgtcgac accgaggcga tggggctcct tccgggccgc gaccggctgt
     2341 gcctcgtgca gatctccgac gggcgcggcg acgagcatct cgtccggttc aatcccggca
     2401 gcgactatgg cgcgcccaac ctcaaggccg tcctcgccga cccggagcgc ctcaagctct
     2461 accatttcgc ccgcttcgac ctcgccatca tgcaggccta tctcggggtc atggccggcc
     2521 cggcctattg caccaagacc gcctcgcgcc tgatccgcac ctataccgac cggcatggcc
     2581 tgaaggagct gatcaaggag cttctccaga tcgacatctc caagcagcag cagacctcgg
     2641 actggggcgc gcccgaattg tcggacgccc agaaggatta tgccgcctcg gacgtgcgct
     2701 acctccacca gatgaaggag aagctggacg aacggctggt tcgcgaaggc cggatgccgc
     2761 tcgccagggc ctgcttcgac ttcctgccgc accgggcgct gctcgatctc gccggctggg
     2821 ccgaggtcga catcttcgct catgtctgat ctcgccgagg accggcgccg ggccaagcgc
     2881 ggctgggccg ccccgggagg aatgcacgac tggctgatgc gcttcctcaa ggtggcgctc
     2941 ccggccggag tcgggctgct cctcgcctat ctcctcctct cgccgctctc caaggagaag
     3001 gagatcagct tccttctcga caagaacaag gtcgacgtcg cgcgcgagcg gctgaagacc
     3061 cagaaggccg aatatcgcgg cctcgacgac aagggccgcg ccttctcgat ccaggcggcg
     3121 caggcggtcc aggccaaatc ctccgatccg gtggtcgaca tcggcggcat ggccgcccgg
     3181 atccagctcc aggaaggccc cgccacgatc accgccgaca aggcgcgcta caatatggac
     3241 acccagaagg tcgacgtgct cggcccgatc ctgatcaccg cacccgacgg ctatcggctc
     3301 gagaccagcg acgtgcaggt cgatctcaac acccaccggc tcggcagcga cgctggtgtc
     3361 gagggccgga tgccgctcgg ccgcttctcg gccgatcaga tgcgcgccga tctgccgtcg
     3421 cgcaaggtcg tgctgagcgg cagggcgcgc ttgcatatcg tgcaggg
//
LOCUS       DASVRK010000199         4093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_36437, whole genome shotgun sequence.
ACCESSION   DASVRK010000199 DASVRK010000000
VERSION     DASVRK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36437"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VF589_07750"
     CDS             <1..590
                     /locus_tag="VF589_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8257512.1"
                     /translation="GWDPALTLSVNISPAQLKDPWLAQKLVKLLVETGFPAERLEVEI
                     TESSLFENLGIAQSIVGSLKNQGIRIALDDFGTGYSSLAHLRALPFDRIKIDRSFVTS
                     INDNPESAAIVNAITRLAESLNLAVTAEGIEHELIEARLRSIGNYKGQGWLFGKPMPA
                     QAVQSLLAERGLLPMRVVEPPRRRMNNRDRLRRHH"
     gene            647..1450
                     /gene="dapF"
                     /locus_tag="VF589_07755"
     CDS             647..1450
                     /gene="dapF"
                     /locus_tag="VF589_07755"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX8257513.1"
                     /translation="MARRFHKMHGLGNDFVIFDARDAPIDMDAARARAIADRMTGIGC
                     DQLILLEPSEAADFKMRIFNADGGEVGACGNAARCVALLVGGERRIETGGGVISGSAA
                     GGEASVDMGEPRFGWDEIPLAYPLDTSALPVGWEALQSPFAVNVGNPHVVFFVEDAGA
                     VDLARLGPEIEHDPLFPERINVNVAQMEDGAIRLRVWERGAGLTRACGTGACAAAVAA
                     ISRKLATSPVEVRLPGGRLGIEWRAGGSIRMTGAASHVFTGELELEAFG"
     gene            1447..2757
                     /gene="mtaB"
                     /locus_tag="VF589_07760"
     CDS             1447..2757
                     /gene="mtaB"
                     /locus_tag="VF589_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX8257514.1"
                     /translation="MSGPELITLGCRLNFAESEAMRRAALGEDDLIIVNSCAVTNEAV
                     RQTRQAIRRAKSARPDARIVVTGCAAQIEPETFAAMPEVSRVVGNREKFDISSFQRKL
                     ESPFPLEEGNEVPAFAGTTRGPDIVVSDIMAVRETAPHLAAGFADRARAFVEVQNGCD
                     HRCTFCIIPYGRGNSRSVPAGLVVEQVQALVDEGYREVVLTGVDLTSYGPDLPGAPTL
                     GMLIERILRHVPALPRLRLSSLDSVEIDPRLEEIIAGEPRLMPHLHLSLQAGDDMILK
                     RMKRRHSRAEAVATVARLKAKRPDIAIGADLIAGFPTETEAMAENSLRLIDDCDIVMG
                     HIFPYSPKRGTPAARMPQVPHAEVKARARRLREATARRRALWLAGLVGTRQRVLAERN
                     GRGHAENFAPVLTGRIAGEGKDVGKVLDVTITGMDGFMLVGEMA"
     gene            2754..3710
                     /gene="ftsY"
                     /locus_tag="VF589_07765"
     CDS             2754..3710
                     /gene="ftsY"
                     /locus_tag="VF589_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX8257515.1"
                     /translation="MSDAPWLERLTAGFRKTSDRLGDNLTGLFTKAALDDAILDDIEE
                     ALIASDLGPATAAKVRERLAGERFERGLSEAAVREIVAEEIAKILAPVAVPLEIDAFP
                     RPQVILVVGVNGSGKTTTIAKLAHLFLEQDYGVLLAAGDTFRAAAIGQLRVWAERIGV
                     PIITGPEGGDPSAIVYDGVKKATAEGIDVLIVDTAGRLQNKKGLMDELAKIRRVLGRL
                     NPEAPHNVVLVLDATTGQNALSQIEVFKEVSGVTGLVMTKLDGTARGGVLAAAAERYR
                     LPIHAIGVGEGMGDLRPFDADEAARAIAGVRAAEDGAGGAAA"
     gene            3707..>4093
                     /locus_tag="VF589_07770"
     CDS             3707..>4093
                     /locus_tag="VF589_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEX8257516.1"
                     /translation="MSENEQEKASAGAKLAIDLGPLVIFFAFNAWKGIFFATGAFMLA
                     IVAAMLVSKLKYRHISPMLWFSGVMVLVLGSITIWLHNETFIKIKPTIYYSTVAALLL
                     FGMYTGRNLLKAVLGTAYPGLSERGWQ"
BASE COUNT          649 a         1383 c         1440 g          621 t
ORIGIN      
        1 gcggatggga cccggcgctg accctgtcgg tcaatatctc cccggcccag ctcaaggacc
       61 cgtggctggc gcagaagctg gtcaagctgc tggtcgagac cggcttcccg gccgagcggc
      121 tcgaagtgga gatcaccgaa agctcgctgt tcgagaatct cggcatcgcg cagtcgatcg
      181 tcggcagcct caagaaccag ggcatccgca tcgcgctcga cgatttcggg accggctatt
      241 cctcgctggc gcatttgcgc gcgctgccgt tcgaccggat caagatcgac cgcagcttcg
      301 tcacctcgat caacgacaat cccgaaagcg ccgccatcgt caacgccatc acccgcctcg
      361 ccgagagcct caacctggcg gtcaccgccg aaggcatcga gcatgagctg atcgaggcgc
      421 ggctgcgctc gatcggcaat tacaagggcc agggctggct gttcgggaag ccgatgccgg
      481 cgcaggcggt gcagtcgctg ctcgccgagc gcgggctgct gccgatgcgc gtggtcgagc
      541 cgccgcgccg gcgcatgaac aatcgcgacc ggctccgccg ccaccactga tcggagccgc
      601 cgccggtccg ctggacttgc cgcccccgcc tccctagatc gcggccatgg cgcgccgctt
      661 ccataaaatg cacgggctcg gcaacgattt cgtgatcttc gacgctcgcg acgcgcccat
      721 cgacatggat gcggcgcgcg cgcgcgccat cgccgaccgg atgaccggca tcggctgcga
      781 ccagctgatc ctgctcgaac cttccgaggc cgccgacttc aagatgcgca tcttcaacgc
      841 cgacggcggc gaggtcggcg cgtgcggcaa tgccgcgcgt tgcgtcgcgc tgctggtcgg
      901 cggcgagcgc aggatcgaga ccggcggcgg cgtcatcagc ggctcggccg cgggcggcga
      961 ggcgtcggtg gacatgggcg agccgcgttt cggatgggac gagatcccgc tcgcttaccc
     1021 gctcgacacc tcggcgctgc cggtcggctg ggaggcgctg caaagcccgt tcgcggtcaa
     1081 tgtcggcaac ccgcatgtcg tcttcttcgt cgaggatgcc ggcgcggtcg acctcgcccg
     1141 gctcggccca gagatcgagc acgatccgct gttcccggag cggatcaacg tcaatgtcgc
     1201 ccaaatggag gacggcgcca ttcgcctgcg cgtgtgggag cgcggcgcgg gcctgacccg
     1261 cgcctgcggc accggcgcct gcgccgccgc ggtggcggcg atcagccgca aactagcaac
     1321 ctcgccggtc gaggtccgtc tccccggtgg gcggctcggc atcgagtggc gcgccggcgg
     1381 ctcgatccgg atgaccgggg cggccagcca cgtcttcacg ggcgagctcg agcttgaggc
     1441 gttcggatga gcggacccga gctgatcacg ctcggctgcc gattgaactt cgccgaaagc
     1501 gaggcgatgc ggcgcgcggc attgggcgag gacgacctca tcatcgtcaa cagctgcgcc
     1561 gtcaccaacg aggcggtgcg ccagacccgc caggcgatcc gccgcgccaa gtcggcgcgt
     1621 ccggacgcgc ggatcgtggt caccggctgc gccgcccaga tcgagccgga gacgttcgcg
     1681 gccatgccgg aagtgagccg ggtggtgggg aatagggaga agttcgatat ttcgtcattc
     1741 cagcgaaagc tggaatctcc cttccccctt gaagaaggaa atgaggttcc agctttcgct
     1801 ggaacgacgc gtgggcccga cattgtcgtc tccgacatca tggcggtgcg cgagaccgcg
     1861 ccgcatctcg ccgccggctt cgcagaccgg gcacgggcct ttgtcgaggt gcagaatgga
     1921 tgcgaccatc gctgcacctt ttgcatcatc ccttatggcc gcgggaacag ccgctcggtc
     1981 ccggccgggc tggtggtgga gcaagtgcag gcgctggtcg acgagggcta tcgcgaggtg
     2041 gtcctgaccg gggtcgacct caccagctac gggcctgacc ttccgggcgc accaacccta
     2101 ggtatgctga tcgagcggat cctgcgccac gtgcccgccc tcccccgcct gcgcctgtcc
     2161 tcgctcgaca gcgtcgagat cgatccgcgc ctcgaggaga tcatcgccgg cgagccgcgg
     2221 ctgatgccgc acctccatct cagcctgcag gccggcgacg acatgatcct gaagcggatg
     2281 aagcgccgcc acagccgcgc cgaggcggtg gcgacggtgg cgcggctgaa ggcgaaacgg
     2341 ccggacatcg cgatcggcgc cgacctcatc gccggcttcc cgaccgagac cgaggcgatg
     2401 gccgagaaca gcctgcgcct gatcgacgat tgcgacatcg tcatgggcca tatcttccct
     2461 tattcgccca agcggggcac gccggcggcg cggatgccgc aggtgccgca tgcggaggtc
     2521 aaggcgcggg cgcggcggct gcgcgaggcg accgcgcgcc gtcgcgcttt gtggctcgcg
     2581 ggcctcgtgg ggacgcgcca gagggtgctg gcggagcgca acggccgcgg ccatgccgag
     2641 aatttcgcgc cggtcctcac cggccggatc gccggcgagg gcaaggatgt gggcaaggtg
     2701 ctggacgtga cgatcacggg gatggacggc ttcatgctgg tgggagaaat ggcttgagcg
     2761 acgcgccctg gctggagcgg ctgacggcgg ggttccggaa gacctccgac cggctcggcg
     2821 acaacctgac cggcctgttc accaaggcgg cgctggacga cgcgattctg gacgacatcg
     2881 aggaggcgct gatcgcctcc gacctcgggc ccgcgaccgc cgccaaggtg cgcgagcggc
     2941 tggccggcga gcggttcgag cgcgggcttt cggaagccgc ggtgcgcgag atcgtggccg
     3001 aggagatcgc caagatcctg gcgccggtgg cggtgccgct cgagatcgac gccttcccgc
     3061 ggccgcaggt gatcctggtg gtcggcgtca acgggtcggg caagaccacc accatcgcca
     3121 agctcgccca cctcttcctc gaacaggatt atggagtgct gctcgccgcc ggcgacacgt
     3181 tccgcgcggc cgcgatcggc cagctccggg tctgggccga gcgcatcggc gtgccgatca
     3241 tcaccgggcc ggagggcggc gacccgtcgg cgatcgtcta tgacggggtc aagaaagcca
     3301 ccgccgaggg catcgacgtg ctgatcgtcg acacggccgg gcggctccag aacaagaagg
     3361 gcctgatgga cgagcttgcc aagatccgcc gcgtgctcgg ccggctgaac ccggaggcgc
     3421 cgcacaacgt cgtgctggtg ctcgacgcca ccaccggcca gaacgcgctc agccagatcg
     3481 aggtgttcaa ggaggtgtcg ggcgtcaccg ggctcgtcat gaccaagctc gacggcaccg
     3541 cccgcggcgg cgtgctcgcg gcggcggccg agcgctaccg cctgccgatc cacgcgatcg
     3601 gggtgggcga gggcatgggc gacctgcgcc cgttcgacgc cgacgaggca gcgcgggcga
     3661 tcgccggcgt ccgcgcggca gaagatgggg cgggaggagc cgcggcatga gcgagaacga
     3721 acaggaaaaa gcgtcggcgg gcgccaagct cgccatcgac ctcgggccgc tggtcatctt
     3781 cttcgcgttc aacgcctgga agggcatctt cttcgcgacc ggcgccttca tgctggcgat
     3841 cgtcgcggcg atgctcgtct ccaagctcaa atatcgccac atctcgccga tgctctggtt
     3901 ctcgggcgtg atggtgctgg tgctggggtc gatcaccatc tggctacaca acgagacctt
     3961 catcaagatc aagccgacca tctattattc gacggtggcg gcgctgttgc tgttcggcat
     4021 gtataccggc cgcaacctgt tgaaggcggt gctcggcacc gcctatccgg ggctgtcgga
     4081 gcgcggctgg cag
//
LOCUS       DASVRK010000200        18799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_475495, whole genome shotgun sequence.
ACCESSION   DASVRK010000200 DASVRK010000000
VERSION     DASVRK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 18799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18799
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475495"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VF589_07775"
     CDS             complement(<1..257)
                     /locus_tag="VF589_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257517.1"
                     /translation="MTEFNGTDGNDELEGSESADTMFGHAGNDSLYGGYGDDQLDGGD
                     GEDFLFGFADDDSMFGAAGDDQLLGSVGNDRLFGGDGNDFL"
     gene            complement(337..2718)
                     /locus_tag="VF589_07780"
     CDS             complement(337..2718)
                     /locus_tag="VF589_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257518.1"
                     /translation="MDEYGTEGDDLFVGDDEGNWYEALGGDDELRGNGGQDDLYGDAG
                     ADLIFGGADNDSLEGGRGSDRLSGGDGDDLLTGGWSFDSADRDKLFGGAGNDVIFGSN
                     GAADELHGEEGADELYGYLGNDQLLGGAGSDYLSDREGADRYDGGEDEGLDWEGSYSF
                     IGDRAQIGGAGGTDNMVVDLRSGIIVNDGFGNRESISGVESLGGGTLFADKFYADDRA
                     NHIVAANRDIVMGFGGDDLVSFSGAVGNFDGGAGVDSLLYMYGSKLIAGAGRGDPPQT
                     LYTDNGVVIDLLAGTVRDGFGETGTIANVENIGGSHGGADDILRGDNKDNVIRGLGGA
                     DVIDGRGGNDTISYWQPEYYGHYGYWHRGEGAVLVDLAAGYAAETALTSGTVTAATPS
                     FGRDSLAGIENIVGTGVGDRLLGNAGNNLIAPDSGSDHVDGRAGTDTVSYDGVRGAVA
                     IDLAAGVGRQRGSGAPAVARVTDEGEHIMVEADDAAASLDRLLNIENAIGSAHGDRLA
                     GTAGSNRLDGGRGDDMLVRSGGGDILVGGLGSDTADYSDAGAAVTIDLAAGSGSVRGI
                     AAADRLLGIENAIGTAFADSLTGSRAANRLDGGAGIDRLDGGAGNDTYVVDDRRDLIA
                     DSAGVDRVEASFSLTLAAGLEQLVLTGAADLGGTGNDAANALTGNAGGNLLAGRGASD
                     RIEGGAGRDAFLFDSALAGAGVDTILDFASADDSIRLDRSFFTGFAETGTLRLAAFRD
                     GARAVDDSDRILYDEATGRIFYDADGSGAAAAILFAKVTPGTDLAAADFVIVG"
     gene            complement(2807..4183)
                     /gene="chrA"
                     /locus_tag="VF589_07785"
     CDS             complement(2807..4183)
                     /gene="chrA"
                     /locus_tag="VF589_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX8257519.1"
                     /translation="MSEPASAHGHNIPLSEAVRVWARIAALSFGGPAGQIAIMHRILV
                     EEKRWIGEQRFLHALNYCMLLPGPEAQQLATYIGWLLHKTKGGLIAGGLFVLPGFLAI
                     MALSWLYAAYGNVGAVEALFFGLKAAVLAIVLEAVRRIGSRALKNNAMRFIAASAFVA
                     IFFFAIPFPLIILAAGMIGYAGRRAGLAAFRGGGGGHGPAGGTAVADADTALGEHVPA
                     HARPSLGWSLRIASVFLFLWLAPVLALVAVLGPDNVFAAIALFFSKMAVVTFGGAYAV
                     LAYVAQQAVETYGWLRPGEMLDGLGMAETTPGPLIIVTQFVGFMGAFRQATGLPPMLA
                     GTLGGILTTWVTFIPCFLWIFLGAPFIEKLRGNEALSSALSAITAAVVGVVLNLAVWF
                     ALHTLFARVREVRAGPFDFDAPILASIDPAAAFLTLAAIVAVFRFRVGTLWLLAGCAA
                     AGLALRSL"
     gene            complement(4180..5823)
                     /locus_tag="VF589_07790"
     CDS             complement(4180..5823)
                     /locus_tag="VF589_07790"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8257520.1"
                     /translation="MKPVWTLEQVTDNLARWNAAWPVGTIDYSYYIFPPGHHQQPVVG
                     FSAFSPAQRTALERIFDLVSDITPLDFREVASNGQEPGEGNERIGFLNVNSSNYFFTG
                     RTRVDFPESDGDALSPIRGADIEINMHRAGPRGGWAIGDSNFNTLNHEVLHSLGLLHP
                     GDYNGDSGHAKDYETLAEYMQDSNQYTVMSYWAAAETGADHVGGGRVRFSATPLLHDI
                     ATLQALYGANMTTRTGNTTYGFNSNAGRASFDLDRDPYLIFAIWDAGGTDTLDLSGFT
                     GSSRIDLAEGAFSSGGDMTDRFAIAHGAVIENAVGGSGADTLLGNAAANRLDGGGGGD
                     RMVGGFGDDVYIVDHSGDRAVENPGGDSGADLVKASVDLTLGARLDNLLLTGAAAIDG
                     TGNALANRITGNNAANALSGDRGGDLIDARGGGDLVAGGAGNDRLRGGGGEDVFLFDA
                     ALSRTSNVDGLLDFSVAQDTIRLDRDIFTGFGRDGRIGAGEFALGARAGGSNDRILYD
                     RDTGRLFYDADGDGAAAAILFAKVTPGTNLTAADFEAYI"
     gene            6123..7838
                     /locus_tag="VF589_07795"
     CDS             6123..7838
                     /locus_tag="VF589_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257521.1"
                     /translation="MALFAGTSANENIGPSFVTESVFSNPAGARPSMADDVINGGGGD
                     DVIDASGGDDIVVGGLGADKAILGQGDDIFRARLGDGHDVVNGGSGYDSYEWTGSDEP
                     LESDYDSAGLELLASGIGLLDHSSGTLNDIERLRFDLGDGIDDLVSLYNHAGSSLERI
                     EIVDAEVVFVEGDIAGERLTVRQQGALLTIAGLQAVVTIENLGDGSLYIAAQDGADSI
                     DLTALGGSGRRVRVEAGDGNDLILAGARAEYLGGGDGSDRISYARSPEGVEIGYEGIA
                     GVGGWAEGDVVFAGESLTGSAFADILGGTGSGNILAGGAGDDRLDALGGFDTLNGGAG
                     GDKLDGGTGGDLMFGGAGDDQYRVDHSGDRMVERAGGGIDLAISAISLTLPAHVENLL
                     FLEGAGPLTGRGNGPANEIHGNEDDNVLQGLGGADVLNGEAGNDLLNGGVGADVTSGG
                     LGDDRIYGGAGADLHRGGFGLDRFFFDTALGGGVDTIDDFVAADDTIMLARERFGEIA
                     AGTLAADAFVEGNAAQDAEDRLIYHKPTGRIFFDADGAGGAAQVLFARVDPATELSNL
                     DFRGY"
     gene            complement(8149..9507)
                     /locus_tag="VF589_07800"
     CDS             complement(8149..9507)
                     /locus_tag="VF589_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257522.1"
                     /translation="MVRLLALAAAAPLAACSAPAPAAPAPAAPDPAFAWVEQATAHFY
                     NAPLRLAWERPGGDWVDAAGLRHGARPFASAALAREGVHDIDVTNLVRAHGADFRVIG
                     RLADIASREAGSGPPLLIVQRGDALGHEAAADVGMDPTTHESRPAAPALKLSDGFYLR
                     FDQPADPSIRRAILRLTVTKVWSGEAIPTQIYRPDPRPAPRAMPDVPLGSEVDVVMRV
                     SGAGWRKGRGWAQSAAHARLGAGGALEAWIPPGGDTALSAIYTIPPAERRELMCLRVD
                     MTIGRDWTAAWGGKFPGLANTGQGDGAADQAGWGGRGADGTRWSARTNRYGHDDSHAF
                     AADWLALGSYAYRVNAETSHGDAVPLAWPVPKGRRFAYDQCVRLNTPGAADGWVAYWI
                     DGRPAGALTGVAWRSHAGAATLPSEIWADIYEGGTGYGAVPHGRHSVTLHALTLSAKR
                     LP"
     gene            complement(9576..10676)
                     /locus_tag="VF589_07805"
     CDS             complement(9576..10676)
                     /locus_tag="VF589_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HEX8257523.1"
                     /translation="MADEQQADGQKQQPRQEPHPPEEKFALTSSRHFPEWLAGTGGSI
                     AFTTYQAGKLFLVGVKPDGRLSVFERTFPRSMGLAVSADGRTLALATQYQIQRFDNVL
                     PPGETDAKGFDALYAPHAGWVTGDVDAHDVAFGPDDRPIFVNTLFGCIAEVADGHSFR
                     PIWKPPFVSRLAAEDRCHLNGFAVADGQPVYATAVSRSDVADGWRDRRTDGGVVVEIA
                     SGEIVCSGLSMPHSPQLHDGRLWLLNSGAGEFGYVDLASGRFEPVAFCPGYARGLAFA
                     GGHALIGLSLARENRTFSGLPLDAALAARDVAPRCGLAIVDLASGDMTGWVRIEGVVR
                     ELYDVSFLPGTRRPALVGFKTDEVKHIISIAD"
     gene            complement(10890..11186)
                     /locus_tag="VF589_07810"
     CDS             complement(10890..11186)
                     /locus_tag="VF589_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257524.1"
                     /translation="MKFSALLAASALLVAGPAAASGKEEIEKANPSNRAAERICKRWI
                     ETGSRLTRTRVCATRAEWQAISDEQQKMVAQHQQNKRTIDSQLGRDAYQQCLRC"
     gene            11465..11791
                     /locus_tag="VF589_07815"
     CDS             11465..11791
                     /locus_tag="VF589_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257525.1"
                     /translation="MDRISAVGAAIVLVACVREPAAGSVDGETATTPNDRAVAQQAER
                     CGIKPDQIIWTTDRSGERYASITPNGDLDGLSFQNLKCILDWGQRTGARVGFISEPPE
                     SAEKAE"
     gene            11791..11973
                     /locus_tag="VF589_07820"
     CDS             11791..11973
                     /locus_tag="VF589_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257526.1"
                     /translation="MDDDPDASLEPGDAISGCLLRLLAFVGLLAIVFAVVAYWGYMRS
                     VERTLGPHTERRAPRL"
     gene            12194..13369
                     /locus_tag="VF589_07825"
     CDS             12194..13369
                     /locus_tag="VF589_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX8257527.1"
                     /translation="MKWFGRKGGAASAGAPRAALRPLLLRGWTGQAPGEPWPRSYEAQ
                     VREAYLSNPVAQRAVRLVAEGAASASVYAVEAAAAAEGGLGGSGARGAAAAALATPGL
                     LETAAAQLLLHGNAFVQIVQDAEGAPAELFALRPERVSVEADAAGWPAAYVYKAGEAR
                     TRLPARDGLGRPALVHLRAMHPLDDHYGLGCLGAAAGAVAVHNAASRWNKALLDNAAR
                     PSGALLYEGGEGELGADQYQRVKAELEDYYQGADNAGRPLLLEGGMRWQAISLSPADM
                     DFVGLKAAAAREIALAFGVPPMLLGLPGDSTYANYREANRALWRLTILPLADKILAGI
                     SAALAAWWPGVRLAVDVDQITALAEDRERLWAQVSAADFLTPEEKREMLGFAASGGG"
     gene            13505..13822
                     /locus_tag="VF589_07830"
     CDS             13505..13822
                     /locus_tag="VF589_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6127 family protein"
                     /protein_id="HEX8257528.1"
                     /translation="MTEANATMLALLVGQAERQGADLVTLRALIEEASEIGAERALGT
                     LGLRDARARRDMDELRELLQAWRDAKKSAARAAIAWMLRIGLALLVLAMAVKLGLHDL
                     VRR"
     gene            13819..14214
                     /locus_tag="VF589_07835"
     CDS             13819..14214
                     /locus_tag="VF589_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEX8257529.1"
                     /translation="MRFAGYAAVFGHPDRGGDVVRAGAFARAVKRGVEAVPLLWQHEP
                     GRPIGRIEYLKEDGRGLRVIGRLSAGAAGREAAALLKDKAVSGLSFGYRVREARGEKP
                     RELVELELVEVSLVTFPMQPKARVHMVGP"
     gene            14241..14834
                     /locus_tag="VF589_07840"
     CDS             14241..14834
                     /locus_tag="VF589_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257530.1"
                     /translation="MEWIAAGLAAMAAPAAAPPPSEYKCHAVAGEYSISSHPIAGGAF
                     AVRGTITPRFLRSHRKWLSTASVIVQGKPGRDAVGVQLIDEGGRNRGRDAVRSVALFL
                     SWYKDGKAGRKYVSSLSREDPVPFAIAIDGNGMARLQIGTGVREMQIDAGGLSEVAMS
                     CSTGTFEFDRMEWTGVSPGAAETDGRRSDAEPVEVIR"
     gene            complement(14802..15500)
                     /locus_tag="VF589_07845"
     CDS             complement(14802..15500)
                     /locus_tag="VF589_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HEX8257531.1"
                     /translation="MRLDSDGARHFPAALTPDDLTALDRLFAAHLPDARPGVRLGRLP
                     ALPPLIGAADAIARAALGALAQPVRAVLFDKSPARNWALGWHQDRTIAVEFASPAGAT
                     PRGHPPRFLEIPGYTGWTLKGGIPHVVPPFEILARMLTLRLHLDHVGDRNAPLLIAPG
                     SHRLGRILEADIAGIVAAHGSLACRAARGDIWAYAAPILHASEAAAEPARRRVLQLYY
                     SADPLADDLDWFGV"
     gene            15715..16839
                     /locus_tag="VF589_07850"
     CDS             15715..16839
                     /locus_tag="VF589_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX8257532.1"
                     /translation="MYEVKADPLEASFEALERESEDVAQLREEMAQLKSRLDAQAVAA
                     ARPALSGAKAEKSPFVESYLRKGLEAGVELKALQGTSDAAGGYAVPEEIDAAIDRTLT
                     AISPIRAIANVVKVGSAGYRKLVTTGGTPSGWVSEVAARPETDTPAFTEIAPPFGELY
                     ANPAASQAMLDDAAFDVEAWLAQEIATEFARAEGAAFVAGSGVNRPRGFLAAPNAATA
                     DGVRPFGTLQFVASGAAGGFVAANPQDRLIDLVQALRPPYRQGAVFVMNSATAARIRK
                     FKTADGAFLWQPGLTAGQPDTLLGYPVVEAEDMPDVAADSLSIAFGNFKAGYLIAERA
                     ETQILRDPYTHKPFVHFYATKRIGGQVSNSEAIKLMKFAA"
     gene            16959..17225
                     /locus_tag="VF589_07855"
     CDS             16959..17225
                     /locus_tag="VF589_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257533.1"
                     /translation="MADAFANVADAVSAPATRAAAVAPHDVNALADVPKALFVGTGGH
                     LMVRGVGGGGDVLFKNVAAGTVLPFRALYVRATGTTAADIVALY"
     gene            17225..17968
                     /locus_tag="VF589_07860"
     CDS             17225..17968
                     /locus_tag="VF589_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257534.1"
                     /translation="MSALGIGLGLALRGRRGGFDPVSLFPGGTAGAFYQIGPGYVWQD
                     MAGTVPAAAGDPVGLLVDRSGNGNHAVQANAAQRPVLRQDAGGRYWLEFDGIDDFLRA
                     GFALAQPWERISALRQTGWTLARHLLGGANVNAGVLQQFGAAANLRLFSGTASSVCNV
                     EPALGADEVISELHDGAASGIAVNNGAPTVADYGAAACGGVTIGGSSAGSSFGNFRLY
                     GLAMRGGPLAAATRTSLRGWMAARAGFTL"
     gene            18018..18602
                     /locus_tag="VF589_07865"
     CDS             18018..18602
                     /locus_tag="VF589_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257535.1"
                     /translation="MLILEPIAAPGAAVAEAKAYLRIELAAEDALVARLIAAAAELCE
                     RFTGQVLLARGFSDMVPVSPAWRRLGATPVRAIVGVEAVAADGTATALGVGDYAIDID
                     AHGDGWVRVTAAGEAKRVRVSGEAGIAADWTALPETLRQGILRLAGHLYAHRDGAADA
                     GPPAAVSALWRPWRRMPFAIGGRRETASPHAWRS"
     gene            18614..>18799
                     /locus_tag="VF589_07870"
     CDS             18614..>18799
                     /locus_tag="VF589_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257536.1"
                     /translation="MLERLLEHGNAKARARAERRAAELAELIGNELPRGATASAAAEG
                     VAVAGRGMSRRFALEPAL"
BASE COUNT         2865 a         5698 c         7483 g         2753 t
ORIGIN      
        1 ttcaggaagt cgttgccgtc gccgccgaac agccggtcgt tgccgaccga gccgagcagt
       61 tgatcgtcgc cggccgcgcc gaacatggaa tcgtcgtcgg caaagccgaa caggaagtct
      121 tcgccgtcgc cgccgtcgag ctggtcgtct ccatacccgc cgtacaggga gtcgttgccg
      181 gcatggccga acatcgtatc ggcagactcg gagccttcga gttcgtcgtt gccgtcggtg
      241 ccattgaatt cagtcacgtg agttccccca aaaaatacag gatgtttctg atctaagccg
      301 gagtgtccga ggatcgcgat gccgcggtga ggcgcctcag ccgacgatga cgaagtccgc
      361 ggcggcaagg tcggtgccgg gcgtcacctt cgcgaacagg atggcggcgg cggcgccgct
      421 gccgtcggca tcgtagaaga tgcggccggt ggcctcgtcg tagaggatgc ggtcgctgtc
      481 atccaccgcg cgggcgccgt cgcggaaggc ggcgaggcgg agcgtgccgg tctccgcgaa
      541 gccggtgaag aaggagcggt ccaggcggat gctatcgtcg gcggacgcga agtcgaggat
      601 cgtgtcgacg cccgcgccgg cgagcgcgct gtcgaacagg aaggcgtcgc ggccggcccc
      661 gccctcgatc cggtcgctgg cgccgcggcc ggcgaggagg ttgccgccgg cattgccggt
      721 gagcgcgttg gcggcatcgt tgccggtgcc gccgaggtcg gcggcgccgg tgaggacgag
      781 ctgctcgagg ccggccgcga gggtcaggct gaagctggct tcgacgcggt ccacgccggc
      841 ggaatcggcg atgaggtcgc ggcggtcgtc gacgacgtag gtgtcgttgc cggcgccgcc
      901 atcgagccgg tcgatgccgg cgccgccgtc gaggcggttg gcggcgcggc tgccggtgag
      961 gctgtcggcg aaagcggtgc cgatcgcatt ctcgatgccg agcaggcggt cggccgccgc
     1021 gatgccgcgg accgagccgg agccggcggc gaggtcgatg gtgacggcgg cgccggcgtc
     1081 cgaataatcg gcggtgtcgc tgccgaggcc gccgaccaaa atatcgccgc cgccggagcg
     1141 gaccagcatg tcgtcgccgc ggccgccgtc gaggcggttg gagccggcag tgccggcgag
     1201 ccggtcgccg tgggcggagc cgatcgcgtt ctcgatgttg agcagccggt cgagcgacgc
     1261 ggcggcgtcg tctgcctcga ccatgatatg ctcgccctcg tccgtgacgc gggctacggc
     1321 gggcgcgccc gatccgcgct ggcggcccac gccggcggcg aggtcgatcg cgaccgcgcc
     1381 cctgacgccg tcatagctga ccgtgtcggt gccggcgcgt ccgtcgacat ggtcgctccc
     1441 ggaatcgggc gcgatcaaat tgttgccggc attgccgagc aggcggtcgc cgacgccggt
     1501 gccgacgatg ttttcgatgc cggcgaggct gtcgcggccg aagctgggcg tggcagcggt
     1561 gacggtgccc gaggtgagcg ccgtctccgc cgcatagccg gccgccagat cgaccaggac
     1621 ggcgccttcg ccgcgatgcc aatagccgta atgtccgtaa tattcgggct gccagtagct
     1681 gatcgtgtcg ttgccgccgc ggccgtcgat cacgtcggcg ccgccgaggc cgcgaatgac
     1741 attgtccttg ttgtcgccgc gcaggatgtc gtcggcgccg ccgtgcgagc cgccgatatt
     1801 ctcgacattc gcaatggtgc cggtctcgcc gaagccgtcg cgcacggtgc cggcgaggag
     1861 atcgatgacg acgccgttgt cggtgtagag cgtctgcggc ggatcgccgc gcccggcgcc
     1921 ggcgatcagc ttgctgccgt acatgtagag gaggctgtcg acgccggcgc cgccatcgaa
     1981 attgccgacc gcgccgctga acgagacgag atcgtcgccg ccgaagccca tgacgatatc
     2041 gcggttggcg gcgacgatgt gattggcgcg atcgtcggcg tagaatttgt ccgcgaacag
     2101 ggtgccgccg ccgagcgact cgaccccgct tatgctttcg cgattgccga agccgtcatt
     2161 gacgatgatt ccggacctca gatcgacgac catgttatcg gtcccgccgg caccgccgat
     2221 ctgggcgcga tcccctatga acgagtagga gccctcccaa tcgaggccct cgtcctcgcc
     2281 gccgtcatac cgatcggcgc cctcccgatc cgacagatag tcgctgccgg cgcctccgag
     2341 cagctggtca ttgccgagat agccgtagag ttcgtctgcg ccctcctcgc catgaagctc
     2401 gtcggcggca ccgttcgatc cgaagatcac gtcgtttccc gcgcctccga acagcttatc
     2461 ccggtcggcg gagtcgaagc tccagccgcc cgtcagcagg tcgtcgccgt cgcctccgga
     2521 aagccgatcg ctgccacggc cgccctcgag actatcgttg tcggcgccgc cgaagataag
     2581 gtcggcgccg gcatccccat agagatcatc ctggccgcca ttgcccctga gttcgtcatc
     2641 gccgccgagc gcttcgtacc aattgccctc atcgtcgccg acgaacaggt cgtcgccttc
     2701 ggtgccatac tcgtccattt actcgattcc ccacacgtcc ccgagcgacg gattgcacga
     2761 ggggtgcgtc cttgtcgatt gaaataaaaa gggaagcgca ggccggtcag aggctccgca
     2821 aagcgaggcc ggcggcggcg cagccggcga gcagccacaa agtgccgacg cggaagcgga
     2881 acacggcgac gatggcggcg agggtgagga aagcggcggc tggatcgatc gaggcgagga
     2941 tcggcgcgtc gaaatcgaac gggccggcgc ggacctcgcg cacgcgggcg aataaagtgt
     3001 ggagcgcgaa ccagacggcc aggttgagga cgacgccgac cacggcggcg gtgatcgcgg
     3061 acagagccga ggatagagcc tcgttgccgc gcagcttctc gatgaatggc gcgccgagga
     3121 agatccagag gaagcagggg atgaaggtga cccaggtggt gagaatgccg ccgagcgtgc
     3181 cggccagcat cggcggcagg ccggtcgcct ggcggaaggc gcccatgaag ccgacgaact
     3241 gggtgacgat gatcagcggg ccaggcgtgg tctcggccat gccgagcccg tcgagcatct
     3301 cgccggggcg cagccagcca taggtctcga ccgcctgctg agcgacgtag gcgagcaccg
     3361 cataggcgcc gccgaacgtg accaccgcca tcttggagaa gaagagcgcg atggcggcga
     3421 agacgttgtc agggccgagg acggcgacga gggcgagcac cggcgccagc cacaggaaca
     3481 ggaagaccga ggcgatgcgc agcgaccagc cgaggctggg gcgggcgtgg gcggggacat
     3541 gctcgccaag cgcggtgtcg gcgtcggcga ccgccgtgcc gccggccggg ccgtggccgc
     3601 cgccgccgcc gcgaaaggcg gcaaggccgg cgcggcggcc ggcatagccg atcatgccgg
     3661 cggcgaggat gatcagcggg aaggggatgg cgaagaagaa gatggcgacg aatgcggaag
     3721 cggcgatgaa gcgcatggcg ttgttcttga gcgcgcgcga gccgatccgg cgcaccgcct
     3781 cgagcacgat cgccagcacc gcggctttga ggccgaagaa caaagcctcg accgcgccga
     3841 cattgccata agcggcgtag agccaggaca gggccatgat ggcgaggaag cccggcagca
     3901 cgaacaggcc gccggcgatg aggccgccct tggtcttgtg gagcagccag ccgatatagg
     3961 tggcgagctg ctgcgcctcg gggccgggga gcagcatgca ataattgagc gcgtgcagga
     4021 aacgctgctc gccgatccac cttttctcct cgacgaggat gcggtgcatg atcgcgatct
     4081 ggccggcggg accgccaaag ctcagcgccg cgatccgcgc ccagacgcgg accgcttcgg
     4141 acagcggaat gttatggccg tgggccgaag ctggctcgct cagatgtagg cctcgaaatc
     4201 ggcggcggtg agattggtgc ccggcgtcac cttggcgaac aggatggcgg cggcggcgcc
     4261 gtcgccgtcg gcatcgtaga agaggcggcc ggtgtcccgg tcgtagagga tgcggtcgtt
     4321 ggagccgccg gcgcgggcgc cgagcgcgaa ctcgccggca ccgatgcggc cgtcgcggcc
     4381 gaagccggtg aagatgtcgc ggtcgagccg gatggtgtcc tgggcgacgg agaaatccag
     4441 gaggccatcg acattggagg tgcggctgag cgcggcgtcg aaaaggaaga cgtcctcgcc
     4501 gccgccgccg cgcagccggt cgttgccggc gccgccggcg acgaggtcgc cgccgccgcg
     4561 cgcgtcgatg aggtcgccgc cgcgatcgcc ggagagcgca ttggcggcgt tgttgccagt
     4621 gatacggttg gcgagcgcgt tgccggtgcc gtcgatggcg gcggcgccgg tgaggagcaa
     4681 attgtcgagc cgggcgccga gggtcaaatc gaccgaggcc ttgacgaggt cggcgccgct
     4741 gtcgccgccg gggttttcga ccgcgcggtc gccgctatgg tcgacgatgt agacgtcgtc
     4801 gccgaagccg ccgaccatgc ggtcgccgcc gccgccgccg tcgagccggt tggcggcggc
     4861 gttgccgagc aatgtgtcgg cgccggagcc gccgaccgca ttctcgatca cggcgccgtg
     4921 ggcgatggcg aagcggtcgg tcatgtcgcc gccgctcgag aaggcgcctt cggcaaggtc
     4981 gatgcgcgag gagccggtga agccggacag gtcgagcgtg tcggtgccgc cggcgtccca
     5041 gatggcgaag atgagatagg ggtcgcggtc gagatcgaag ctggcgcggc cggcgttgga
     5101 attgaagccg taggtggtgt tgccggtgcg ggtggtcatg ttggcgccgt agagggcctg
     5161 gagcgtggcg atgtcgtgca agagcggcgt ggcggagaag cgcacgcggc cgccgccgac
     5221 atgatcggcg ccggtttccg cggccgccca ataggacatc accgtatatt ggttgctgtc
     5281 ctgcatatat tcggcgagcg tctcgtaatc cttggcgtgg ccgctgtcgc cattatagtc
     5341 gcccgggtgg agcaggccga gcgagtgcag cacctcgtgg ttcaaggtgt tgaagttgga
     5401 atcgccgatc gcccagccgc cgcgcgggcc ggcgcggtgc atgttgatct cgatatccgc
     5461 gcctcggatg gggctgagcg cgtcgccatc ggattcgggg aagtcgacgc gggtgcggcc
     5521 ggtgaagaaa taattgctgg aattgacgtt gaggaagccg atccgctcgt tgccctcgcc
     5581 cggctcctgg ccgttgctcg cgacctcgcg aaagtcgagc ggggtaatgt cggagacgag
     5641 atcgaagatg cgctcgagcg cggtgcgctg ggccggcgaa aaggcgctga agccgacgac
     5701 cggctgctga tgatggccgg gcgggaagat gtagtagcta taatcgatcg tgccgaccgg
     5761 ccaggcggcg ttccagcggg cgagattgtc ggtgacctgt tcgagcgtcc agacgggctt
     5821 catcgcaatc ccttccacga agatgcgcga tagtgatgtg aagccgggcg catgacaatg
     5881 gcgattcgcc gcaaccgctc aagaaagggc ctgttcgcag cgatttgcga cgaatgtcgc
     5941 tggacacccg cgaacgtcac gggcggcggc cggcaggttt tgcgaccgaa gccgcggcgg
     6001 ggcctgccaa tatgacgctg aacgcggcga agcgattcga cgcggggggc cgcttcggct
     6061 tattctgacc cgtttccgat cgagcctcgg cccggcccgg ctcgcaacga ggggagatgg
     6121 acatggcttt attcgcgggc accagcgcga acgagaacat cggaccttcg ttcgtgacgg
     6181 aaagcgtgtt cagcaacccg gcgggcgcaa gaccctcgat ggccgacgac gtcatcaacg
     6241 gcggcggcgg cgacgatgtg atcgatgcga gcggcggcga cgatatcgtg gtcggcgggc
     6301 tcggcgccga caaagcgatc ctcggccagg gcgacgacat cttccgggcg cggctcggcg
     6361 acggccatga tgtggtcaat ggcggctcgg gctacgattc ctacgaatgg accggctcgg
     6421 acgagcccct cgagtccgat tatgacagcg ccggcttaga gctgctcgcc agcgggatcg
     6481 gcttgctcga ccattcgagc ggaaccctca acgacatcga acggctgcgg ttcgatctgg
     6541 gcgacggcat cgacgacctc gtctcgcttt acaaccacgc cggcagcagc ctcgagagga
     6601 tcgagatcgt cgacgccgag gttgtcttcg tggaaggcga catcgccggc gagcggctga
     6661 ccgtgcggca acaaggcgcg ctgctgacca tcgccggcct gcaggcggtc gtgacgatcg
     6721 agaatctcgg cgacggctcc ttgtacatcg ccgcccagga cggcgccgac agcatcgacc
     6781 tgaccgcgct cggcggctcc gggcggcgcg tgcgcgtcga ggccggcgat ggcaacgacc
     6841 tcatcctggc aggcgcgcgg gcggagtatc tgggcggtgg ggacggcagc gacaggatca
     6901 gctatgcgcg ctcgcccgag ggcgtggaga tcggctatga gggcattgcg ggcgtcggcg
     6961 gatgggccga aggcgacgtg gtgtttgccg gggagagcct caccggctcg gccttcgccg
     7021 acattctcgg cgggaccggg tccggcaaca tattggcggg cggggccggg gacgaccggc
     7081 tcgacgcgct cggcgggttc gacacgctca acggcggcgc cggcggcgac aagctggacg
     7141 gcggcacggg tggcgacctg atgttcggcg gcgccggcga cgaccaatat cgggtcgacc
     7201 attcgggcga ccgcatggtc gagcgcgcgg gcggcggcat cgacctcgcg atcagcgcga
     7261 tatcgctgac gctccccgcc catgtcgaga atttgctctt cctggagggc gccggcccgc
     7321 tgaccggccg gggcaacggc cccgccaacg agatccacgg caatgaggac gacaatgtgc
     7381 tgcaggggct gggcggcgcc gacgtgctga acggcgaggc cggcaacgat ttgctgaacg
     7441 gcggcgtcgg cgccgacgtg acgtcgggcg gcctcggcga cgaccggatc tatggcggcg
     7501 ccggagcgga cctgcatcgc ggcggcttcg gcctcgaccg cttcttcttc gacacggcgc
     7561 tcggcggcgg cgtcgacacg atcgacgact tcgtggccgc cgacgacacg atcatgctcg
     7621 cgcgggagcg gttcggcgaa attgcggcgg gaacgctggc cgccgacgcg ttcgtggagg
     7681 gcaatgccgc ccaggatgcc gaggaccggc tgatctatca caagccgacc ggccgcatct
     7741 tcttcgacgc cgacggcgcc ggcggggcgg cccaggtgct gttcgcgcgg gtcgatccgg
     7801 cgacggagct tagtaatttg gacttcaggg ggtattgaga ggggggggga gagcctcggc
     7861 tggtcatccc ggatcccccc gcaataccag gtccccgcgc catcccagcg aaagctggga
     7921 tctctaaatc ctcccctgct tttgcagggg agggggaccg tccgtaggac ggtggagggg
     7981 cggcgcgcag cgcctctttg cccaagcccg aagagctcgc ttcgctcgcg cccctccacc
     8041 atgctgcgca tggtccccct cccccggaaa gccgggggag gagctaagag gcccctccac
     8101 cgcgctgcgc gcggtcccct ccgccggaaa gccgggggag gaatttcact acggaagccg
     8161 tttggcgctt agggtgaggg cgtggagggt gacactgtgg cggccgtggg ggacggcgcc
     8221 gtagccggtg ccgccttcgt aaatgtcggc ccagatttcg gaggggaggg tggcggcgcc
     8281 ggcgtggctg cgccaggcca cgccggtcag cgcgccggcc gggcggccgt cgatccaata
     8341 ggcgacccag ccgtcggcgg cgccgggggt gttgaggcgg acgcattgat cgtaggcgaa
     8401 gcggcggccc ttgggcaccg gccaggcgag cgggacggcg tcgccgtggc tggtctcggc
     8461 attgacccgg taagcgtagc tgccgagcgc gagccagtcg gcggcgaagg catggctgtc
     8521 gtcatggccg tagcggttgg tgcgggccga ccagcgggtg ccgtcggcgc cgcggccgcc
     8581 ccagccggcc tggtcggcgg cgccgtcgcc ctggccggta ttggcgaggc cggggaactt
     8641 gccgccccag gccgcggtcc agtcgcggcc gattgtcatg tcaacgcgca ggcacatgag
     8701 ctcgcggcgc tcggccgggg ggatggtgta gatggcggag agcgcggtgt cgccgccggg
     8761 cgggatccag gcttcgagcg cgccgcctgc cccaagccgg gcatgggcgg ccgactgcgc
     8821 ccagccgcgg cccttgcgcc agccggcgcc ggagacgcgc atcaccacgt cgacctcgga
     8881 gccgagcggc acgtcgggca tggcgcgcgg ggcgggacgc ggatcggggc ggtagatttg
     8941 ggtggggatc gcttcgcccg accagacctt ggtgacggtg aggcggagga tggcgcggcg
     9001 gatcgagggg tcggccggct gatcgaagcg gaggtagaaa ccgtcggaaa gcttcagcgc
     9061 gggggcggcg gggcggcttt catgggtggt gggatccatg ccgacgtcgg cggcggcctc
     9121 gtggccgagc gcgtcgccgc gctggacgat gagcaagggc ggaccggagc cggcctcgcg
     9181 gctggcgata tcggcgaggc ggccgattac gcggaagtcg gcgccgtggg cgcggaccag
     9241 gttggtgaca tcgatgtcgt ggacgccttc gcgggcgaga gcggcggagg cgaatgggcg
     9301 ggcgccgtgg cggaggccgg cggcgtcgac ccagtcgccg cccggccgct cccaggcgag
     9361 ccgcagcggc gcgttgtaga aatgcgcggt ggcctgctcg acccaggcga aggcgggatc
     9421 gggcgcggcc ggggcgggcg ccgcgggcgc gggggccgaa caggcggcga gcggcgcggc
     9481 ggcggcaagg gcaaggagac ggaccatgcg ccagcgctag cagaaggcga ttaccgaaag
     9541 ctttgcggcc cgtcggcgcg ggaggcgggg ggcggtcagt cggcgatgga gatgatgtgc
     9601 ttcacctcgt cggtcttgaa gccgaccagg gcggggcggc gggtgccggg aaggaagctc
     9661 acatcataga gttcgcgcac gacgccttcg atgcggaccc agccggtcat gtcgcccgag
     9721 gcgagatcga cgatggcgag gccgcagcgc ggcgcgacgt cgcgggcggc cagcgccgcg
     9781 tcgagcggaa ggccggagaa ggtgcggttc tcgcgcgcca gcgacaggcc gatgagggca
     9841 tggccgccgg cgaaggcgag gccgcgggca tagccggggc agaaggcgac cggctcgaac
     9901 cggcccgagg cgagatcgac atagccgaac tcgccggcgc ccgaattgag cagccacagc
     9961 cggccgtcat ggagctgcgg cgaatgcggc atcgacaggc cggagcagac gatctcgccc
    10021 gaagcgattt cgacgacgac gccgccgtcg gtgcggcgat cgcgccagcc gtcggcgacg
    10081 tcggagcgcg acacggcggt cgcataaacg ggctgcccgt cggcgaccgc gaagccgttc
    10141 aaatggcagc ggtcctcggc ggcgaggcgg gagacgaagg gcggcttcca gatcggacgg
    10201 aagctgtgac cgtcggcgac ctcggcgatg cagccgaaca atgtgttgac gaagatcggc
    10261 cggtcgtccg gcccgaaggc gacgtcatgg gcgtcgacgt cgccggtcac ccagccggcg
    10321 tggggggcgt agagcgcgtc gaagcccttg gcgtcggttt cgcccggcgg cagcacattg
    10381 tcgaagcgct ggatctgata ttgggtggcg agcgcgagcg tacgcccgtc cgcgctgacc
    10441 gcgagcccca ttgaccgcgg gaaggtgcgt tcgaacaccg acaggcggcc gtcgggcttg
    10501 acgccgacca ggaacaattt gccggcctgg taggtggtga aggcgatcga gccgccggtg
    10561 ccggcgagcc attcgggaaa gtggcgcgag ctggtgagcg cgaacttttc ctcgggcggg
    10621 tgcggttcct ggcgcggctg ctgcttttgc ccgtccgcct gctgctcgtc cgccatctcg
    10681 ctcccttcgc cgccgtcgac gagcgaacct agcagcgccg gcgggcggtg gacagggggc
    10741 ggatcgggcg gaggcgcggg gtgcgggaca ggtgggcggc ggcggccggg cggcggctca
    10801 ggcgtcgcgg caggcgcgcg ccggctgggg gcggcgccgt tcgggcggaa gaattggcta
    10861 gcggcgggct gggccgccgc cgcgcgcggt cagcagcgca ggcattgctg ataggcgtcg
    10921 cggccgagct gactgtcgat cgtgcgtttg ttctgctggt gctgggcgac catcttctgc
    10981 tgctcgtcgg aaatggcctg ccattcggcg cgggtggcgc agacgcgggt gcgggtgagg
    11041 cggctgccgg tctcgatcca gcgcttgcag atccgctcgg cggcgcggtt ggaaggattg
    11101 gccttttcga tctcttcctt gccgctggcg gcggcggggc cggccaccag caaagcggac
    11161 gccgccaaca gagcggagaa tttcatgtcg cctcctgtcg ttccaactga tcgagccggg
    11221 ccacagtatc acgcgtcggg cggttcgcct aacaagcccc ggacgcgggt ttcccggggg
    11281 ccgcggcggc tggagataag agcgtatctg gtaaaacagc ttgacagcgt gacgctgtta
    11341 tggtaccgaa tcgtcaagct ggaaaattgt agctgaggtc gcggagcggg ccaagggctg
    11401 gtgcgctgtt gctctatgcc gcccacgtga agcggaagct tgaacccgtc ggggagccgg
    11461 agtgatggat cggatctcag ccgtgggagc cgctatcgtg ctggtggcgt gcgtccgcga
    11521 acctgctgcc ggctctgtcg acggtgagac ggcgaccaca cctaacgatc gcgctgtcgc
    11581 gcagcaggcg gagcgatgcg gaatcaagcc cgaccagatc atctggacga cggatcgctc
    11641 gggggagcgt tacgccagca tcactccgaa cggcgatcta gacggcctgt cattccagaa
    11701 cctcaaatgc atcctcgatt gggggcagcg aacgggtgct cgggttggct ttatctccga
    11761 accgcctgaa tcggcggaga aggcggagtg atggatgacg atccggatgc aagcctggag
    11821 ccgggcgacg cgatttcagg atgtctgttg aggttattgg cctttgtggg actgctcgcc
    11881 atcgtcttcg ccgtcgtcgc ctattgggga tatatgagaa gcgtcgagcg caccctgggt
    11941 ccgcacaccg agcgaagggc accgagactg tagctctagc tcatgtggcg actgaatcag
    12001 ccgaggggag aattgtttgt tgccgcccac gaaagaggcg aggctcataa ccggaagggt
    12061 aaacaacact tgacagcgtg acgctgttat ggtacccaat agtcaagctg gaaaattgta
    12121 gctggttcgc gaggatcgag gaaaaggccg gggcggcgac gccttccggc ctttttggcg
    12181 tgggggtgac aggatgaagt ggttcggcag gaaggggggc gcggcttcgg cgggcgcacc
    12241 gcgggcggcg ctgaggccgt tgctgctgcg gggctggacc ggccaagcgc cgggcgagcc
    12301 gtggccgcga tcctatgagg cgcaggttcg cgaagcctat ctctccaacc cggtggcgca
    12361 gcgggcggtg cggctggtcg cggaaggcgc ggcctcggcc agcgtctatg cggtcgaggc
    12421 cgccgcggcc gccgaagggg gattgggggg atcgggcgcg cgcggtgcgg cggcggcggc
    12481 gctggcgacg ccggggctgc tggagaccgc ggcggcgcag ctgctgctgc acggcaatgc
    12541 gttcgtgcag atagttcagg atgccgaggg ggcgccggcg gaattgttcg cgctgcggcc
    12601 ggagcgggtc agcgtcgagg ccgacgcggc gggctggccg gcggcctatg tctacaaggc
    12661 gggcgaggcg cggacgcggc tgccggcgcg ggacgggctc gggcggccgg ccctcgtcca
    12721 tttgcgggcg atgcacccgc tcgacgatca ttatgggctc ggctgcctcg gcgcggcggc
    12781 gggcgcggtg gcggtgcaca atgccgccag ccgctggaac aaggcgctgc tcgacaatgc
    12841 cgcgcggccg tcgggggctt tgctgtacga gggcggcgag ggcgagttgg gggcggacca
    12901 gtatcaaagg gtcaaggccg agctcgaaga ttattaccag ggcgcggaca atgccgggcg
    12961 gccgctgttg ctcgagggcg ggatgcgctg gcaggcgatc agcctgtcgc cggccgacat
    13021 ggatttcgtc gggctgaaag cggcggcggc gcgggagatc gcgctcgcct tcggagtgcc
    13081 gccgatgctg ctcgggctgc cgggggattc gacctacgcc aattatcgcg aggccaaccg
    13141 ggccttgtgg cggctcacca tcctgccgct ggcggacaag atattggccg ggatttcggc
    13201 ggctctggcg gcgtggtggc cgggggtgag gctggcggtg gacgtcgacc agatcaccgc
    13261 gctggccgag gaccgcgagc ggctgtgggc gcaggtgagc gcggccgatt tcctgacccc
    13321 ggaggagaaa agggagatgc tgggatttgc ggcgtcgggc ggggggtgat gtggctgcgg
    13381 gggctgcggg gccggaaggg gaaggcggat cccggctttc gccgggatga cggagagctt
    13441 tcgctgggat gacgtgggtt cgggtgacgg ggtgcctgcg aggggcgtgg tttgggagtg
    13501 gagtatgacg gaagcgaatg cgacgatgtt ggcgctgctg gtcggacagg ccgagcggca
    13561 gggggccgat ctcgtcacct tgcgcgcgct gatcgaggag gcgagcgaga tcggggcgga
    13621 acgggcgctg gggacgctgg ggctgcgcga cgccagggcg cggcgcgaca tggacgaatt
    13681 gcgcgagctg ctgcaggcct ggcgcgacgc caagaaatcg gcggcgcggg cggcgatcgc
    13741 ctggatgctg cgcatcggcc tcgccctgct ggtgctggcg atggcggtga agctcgggct
    13801 tcacgatctg gtgcggcgat gaggttcgcg ggctatgccg ccgtgttcgg ccatcccgac
    13861 cgcggcggcg acgtggtgcg ggcgggagcg ttcgcgcgcg cggtgaagcg gggggtggag
    13921 gcggtgccgt tgctgtggca gcacgagccg gggcggccga tcgggcggat cgaatatctg
    13981 aaggaggacg gacgcgggct gcgggtgatc gggcggctgt cggcgggcgc cgccgggcgc
    14041 gaagcggcgg ctttgctcaa ggacaaggcg gtgagcggcc tcagcttcgg ctaccgggtg
    14101 cgcgaggcgc ggggggaaaa gccgcgcgag ctggtcgagc tggagctggt ggaggtgagc
    14161 ctggtaactt tcccgatgca gccgaaggcg cgggtccata tggtggggcc ctgaggccgt
    14221 tacgaggcga gggtgcgatg atggaatgga ttgcagccgg gctcgcagcg atggcagccc
    14281 ccgccgccgc gccgccgccg agcgagtata agtgccatgc ggtcgcgggc gaatattcga
    14341 tatcgtcgca ccccatcgcg ggcggggcgt tcgcggtgcg cggcacgatc acgccgcgct
    14401 tcctgcgcag ccaccggaaa tggctgtcga ccgcatcggt gatcgtccag ggcaagcccg
    14461 gccgggatgc ggtgggggtg cagctgatag acgagggcgg gcgcaaccgc ggccgcgacg
    14521 cggtgcgatc ggtcgccctg ttcctcagct ggtacaagga cggcaaagcc gggcgcaaat
    14581 atgtttcgag cctgtcgcgg gaagacccgg tgccgttcgc cattgcgatc gacgggaacg
    14641 gcatggcgcg gctgcagatc ggcaccggcg tccgcgaaat gcagatcgac gcgggggggt
    14701 tgagcgaggt ggcgatgtcc tgctcgaccg ggacgttcga gttcgaccgg atggaatgga
    14761 cgggagtgtc gcccggcgcc gcggaaacgg atggccggcg atcagacgcc gaaccagtcg
    14821 aggtcatccg ctagcgggtc ggcgctgtaa tagagctgga ggacgcggcg gcgggcgggc
    14881 tcggcggcgg cttcggaggc gtggaggatc ggcgccgcat aggcccagat gtcgccgcgg
    14941 gcggcgcggc aggcgaggct gccatgggcc gcgacgatgc cggcgatgtc ggcttccagg
    15001 atgcggccga gacggtggct gccgggcgcg atcagcagag gggcgttgcg gtcgccgaca
    15061 tggtcgaggt ggaggcgcag ggtgagcatg cgcgcgagga tttcgaacgg cggcacgacg
    15121 tgggggatgc cgcccttgag cgtccagccg gtgtaaccgg ggatttcgag gaagcgaggc
    15181 gggtggcctc gcggggttgc tcccgccggg gaagcgaact cgaccgcgat ggtgcggtcc
    15241 tggtgccagc cgagcgccca gttgcgggcc gggctcttgt cgaaaaggac ggcgcggacg
    15301 ggctgggcga gcgcgccgag cgcggcgcgc gcgatggcgt cggcggcgcc gatcagcggc
    15361 ggcagggcgg ggaggcggcc gagccggact ccgggacggg cgtccggcag gtgcgcggcg
    15421 aacaggcggt cgagcgccgt gaggtcgtcg ggggtgaggg cggcggggaa gtgccgggcg
    15481 ccgtcggagt cgagccgcat cggatgggga tagccggtcc gggggcgaag ggaacgggga
    15541 tttgacgttg gctgccgggg ggtggccggg ggaaggaagc gggatcccgg atcaagtccg
    15601 ggatggcgtg ggtcggatca agtccgggat gagaagaagc gggatcccgg atcaagtccg
    15661 ggatgacgaa ggggtccacg gcgtgggcat ggttcaacga gcgggagacg ggaaatgtat
    15721 gaagtgaagg cggatccgct ggaggcgtcg ttcgaggcgc tggagcggga gagtgaggat
    15781 gtggcgcagc tgagggagga gatggcgcag ctcaaaagcc ggctggacgc gcaggcggtg
    15841 gcggcggcgc ggccggcgct gagcggggcg aaggcggaga aatcgccgtt cgtcgaaagc
    15901 tatttgcgca aggggctcga ggccggggtc gaactgaagg cgctgcaggg caccagcgat
    15961 gcggccggcg gctatgcggt gccggaggag attgatgcgg cgatcgaccg gacgctgacc
    16021 gcgatctcgc cgatccgggc gatcgccaac gtcgtgaagg tcgggtcggc gggctatcgc
    16081 aagctggtca cgaccggcgg cacgccgtcc ggctgggtgt cggaagtggc ggcgcggccg
    16141 gagaccgaca cgccggcctt caccgagatc gcgccgccgt tcggcgagct ttacgccaat
    16201 ccggcggcga gccaggcgat gctcgacgat gccgcgttcg acgtcgaggc gtggctggcg
    16261 caggagatcg cgaccgaatt cgcgcgggcc gagggggcgg cgttcgtcgc cggcagcggc
    16321 gtcaaccggc ccaggggctt cctggcggcg ccgaacgcgg cgaccgcgga cggggtgcgg
    16381 ccgttcggga cgctgcagtt cgtggcttcg ggcgcggccg gcgggttcgt ggcggccaac
    16441 ccgcaggacc ggctgatcga cctcgtccag gcgctgcggc cgccttaccg gcagggcgcg
    16501 gtgttcgtga tgaattcggc gacggcggcg cggatccgca agttcaagac cgcggacggg
    16561 gcgttcctgt ggcagccggg gctgacggcg gggcagccgg acactttgct cggctatccg
    16621 gtggtcgagg cggaggacat gcccgacgtc gccgccgaca gcctctcgat cgcgttcggc
    16681 aatttcaagg cgggctatct gatcgccgag cgggcggaga cgcagatcct gcgcgatccc
    16741 tatacccaca agccgttcgt gcatttctac gcgaccaagc ggatcggcgg acaggtcagc
    16801 aattcggaag cgatcaagct gatgaagttc gcggcctagc gtgccctcac ctcccaccgc
    16861 tggcgcggtg ggcccctccc tctccccgaa cgggagaggg gtatttgtgc gccgttccgg
    16921 tggtggccgg ggcggcgctt ttgtctggga gagacgagat ggctgatgca ttcgcgaacg
    16981 tcgccgatgc agtgagcgcg ccggcgacgc gggcggcggc ggtggcgccg cacgatgtca
    17041 acgctttggc ggacgtgccc aaggcgctgt tcgtcggcac gggcgggcat ttgatggtgc
    17101 gcggcgtggg cggcggcggc gacgtgctgt tcaaaaacgt ggcggcgggc acggtgctgc
    17161 cgttccgcgc gctttatgtg cgggcgaccg gcacgacggc ggccgatatc gtggcgcttt
    17221 actgatgagc gcgctcggga tcgggctcgg cctcgcgctt cgcgggcggc gcggcgggtt
    17281 cgacccggtg tcgctgttcc ccggcgggac ggcgggcgcc ttttaccaga tcgggccggg
    17341 ctatgtctgg caggatatgg cggggacggt gccggcggcg gccggcgacc cggtcgggct
    17401 gctggtcgac cggtccggca acggcaacca tgcggtgcag gcgaacgcgg cccagcggcc
    17461 ggtgctgcgg caggatgcgg gcgggcgcta ttggctcgaa ttcgacggga tcgacgattt
    17521 cctgcgcgcc ggcttcgcgc tggcgcagcc gtgggagcga atctcggcgc tgcggcagac
    17581 cgggtggacc ctcgcccggc atttgctggg cggggcgaac gtcaatgctg gcgtgctgca
    17641 gcaattcgga gcggcggcga acctcaggct gttcagcggc accgcctcgt cggtgtgcaa
    17701 cgtcgagccg gcgctcggcg cggacgaggt gatttcggag ctccatgacg gcgccgccag
    17761 cggcatcgcg gtcaacaatg gcgcgccgac cgtcgccgat tacggggcgg ccgcctgcgg
    17821 cggggtgacg atcggcggat cgagcgcggg atcgtcgttc ggcaatttcc ggctgtacgg
    17881 gctggcgatg cgcggcgggc cgctggcggc ggcgacgaga acgagcctgc gcggctggat
    17941 ggcggcgcgc gcgggcttca cgctttgagg agagacgaga tgagccagcg cgaagagcgg
    18001 gcggcgggag cggcgccatg ctgattctgg agccgatcgc cgcgccgggc gcggccgtgg
    18061 ccgaagccaa ggcctatctg cgcatcgagc tggccgcgga ggacgcgctg gtggcgcggc
    18121 tgatcgcggc ggcggcggag ctgtgcgagc ggttcaccgg gcaggtgctg ctggcgcgcg
    18181 ggttcagcga catggtgccg gtgagccccg cctggcggcg gctcggcgcg acgccggtgc
    18241 gggcgatcgt cggcgtcgag gcggtggcgg cggacgggac tgccacggcg ctcggcgtgg
    18301 gcgattatgc gatcgacatc gacgcccatg gcgacggctg ggtgcgggtg accgcggcgg
    18361 gggaggcgaa gcgggtgcgg gtgagcgggg aggcggggat cgccgccgac tggacggcgc
    18421 tgcccgagac gctgcggcag gggatattgc ggctggcggg gcacctttac gcgcatcgcg
    18481 acggagccgc cgatgcgggg ccgccggcgg cggtgtcggc gctgtggcgg ccgtggcggc
    18541 ggatgccgtt cgcgatcggc gggcggcgcg aaactgcatc gccgcacgcc tggcggagct
    18601 gaaggaggcg gcgatgctgg agcggctgct ggagcatgga aatgccaagg cccgcgcgcg
    18661 ggccgagcgc agggcggcgg agctggcgga gcttattggg aacgagctgc cgcggggggc
    18721 gaccgcctca gcggcggcgg agggcgtggc cgtggcgggg cggggcatga gccgccggtt
    18781 cgcgctcgag ccggcgctg
//
LOCUS       DASVRK010000201         6576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_413327, whole genome shotgun sequence.
ACCESSION   DASVRK010000201 DASVRK010000000
VERSION     DASVRK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413327"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VF589_07875"
     CDS             complement(<1..1584)
                     /locus_tag="VF589_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX8257537.1"
                     /translation="MAATPQAETGLAPARLWALFRRRWPILVAIVVATTLAAFLYSSL
                     VLARDPTFKASATLDVSPSRAELEYANQFVRNTGLQSAGVLTQTYAEYAMSRPVLASV
                     IDEYIAKVPAALQPQPQTGLGLRRMWNSLNYGGVPVQNPREGLIDALTEATRVATVNG
                     TYLLRIDVEWDNRRLAAWFANEITDRLMAAAAVRTELPADRLNRQLAERLAQTRATLE
                     DKQRQAVATRGSLGVADIPAQRQAIVEERLAEEARLTGEMAQLSSSTAQVGVLQRQAG
                     GKLSASTPAVDQALALERPRAAGLAQAAAQRRARIAGLNGQLDRVARAEATISALDRD
                     IAILQVEATALAERASQVQLDRLAVGPTLRVVERATPPLVRDSPKVMVNTAMGFIAGC
                     ALAGMIMLLAPAPATVAAVRRRRTTVRSDAGPRSYPGVLKAPRSGGSFSAKESRDIKA
                     KLESWLAEPLMQPGRPLYVLGAGQDADATALFNLLRAFLQARGERVAAISALHHELPP
                     AGAADGDERVRPLIFCGGLA"
     gene            complement(1616..2380)
                     /locus_tag="VF589_07880"
     CDS             complement(1616..2380)
                     /locus_tag="VF589_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX8257538.1"
                     /translation="MRNLFALSALAALLTGCFASTNYDLPAPIALTQGSRLAAQQANL
                     RIQEQLGAGGDFHPGDLVRISFPYLPTLNSDQRVQPSGFISPPLLDPIQTRGLTAAQL
                     QQRLEQLYEPKLERPSVELAMVEYNQKPAPPEYFVLGEVIRPGAIAYREGTTLLEAIA
                     RAGGSNRQAKLSTVVVVEPRGEQMLARLVDVQALFSGHDDGRARTLDYISPYTVIIVP
                     PTNLVLSADRSETIRRVLGFTGFGTGFRIGLGGENN"
     gene            complement(2497..3639)
                     /locus_tag="VF589_07885"
     CDS             complement(2497..3639)
                     /locus_tag="VF589_07885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8257539.1"
                     /translation="MRPLRLAFLLPHFRPGGAERVVLNLLRALDRGRFTPHLFLSHAE
                     GAFLDLLPDDVQPVELGAARARHLPRRLRHTLGAAGIDIAYSATNAMNLALLASAIGR
                     GGGNALRIVSEHTSPDAYLAEAKHPGLRRLLMRHLYPRADAIAVPTPGIAEELGKVLG
                     RADLPTLVLPNPVVDAVRLRDRPGTAGKVRIISAGRLVPAKGFDLLLDACAMLDRRGV
                     DFELAIFGEGPERAALQARIGELNLAGKASLAGHASDLGAELGRADLFVLASRREGFG
                     NVIVEAMAARVPVLATACDGPRSLISDGENGFLVRPGDTEALAEAMESLVNSPERRAS
                     VIGAAAATANRFEVFEATGIFERALLDLAARARPGAVPRGDTSRLP"
     gene            complement(3636..4721)
                     /locus_tag="VF589_07890"
     CDS             complement(3636..4721)
                     /locus_tag="VF589_07890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8257540.1"
                     /translation="MIPERLKTVLAAELSEEDRAAFDAFVGGSPYSAYQQSRAWAEHA
                     PRARRQDFLYFLCRDGEALVGAGVVRRSRLGPLARLATLQRGPVVHDVERLGAVVAAL
                     KHALRQAGFSTLVLGPRIGGDERAEALRILKGQGLRPLPADRQSLHTATGKIDLGRGE
                     EAVLAGFKQRGRRQIRAAAKQGVTVRPAVSAEDLAAYQAVLSDFQRRKSRYETAGLPD
                     VLQQAAIVRANGGAILIAEREGAVIGGHAFVVQGADAIWLSMATSDLDPAIPRSYALL
                     WEAIRLARTMGCRSYDLAGMSEGESGDEGEASRDQFKGAFAPQIDRLVPAHVAPLRPL
                     RHLVFFNGRQIYRGLSRSLRHLRSRAA"
     gene            4792..5814
                     /locus_tag="VF589_07895"
     CDS             4792..5814
                     /locus_tag="VF589_07895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8257541.1"
                     /translation="MLVPFHLSESWFDCWFAAYRNTPAAYLPVAEGRLYHVQDKRKVG
                     PLSFKVTRGATNLQTAYFDICGTGDAEAAAGAAARLIEATGADMLEFDYVLKDALLYR
                     AAETWAGSGWTSIEPLGRTALVDCSGRFDDWLAGRRKSDRRAWLKSERQMPERGLSYE
                     LVTGAAEAESAIPELLDVEASGWKGAAGSAIRHNPEDAAFYASLTRRAAEQGALHLAM
                     VRHEGRAVAFDYGILSGDRCLALKASYREEYKVQSIGHVAAMKHVRDMFARDGIRYYD
                     NMGNGLTPQRHKVRFATHYQLFYRIRVFAPTARGLLLREACRAYRGARAVRDRIRPKP
                     SFEPSL"
     gene            5847..>6576
                     /locus_tag="VF589_07900"
     CDS             5847..>6576
                     /locus_tag="VF589_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8257542.1"
                     /translation="MTQWASIKGVGAAAGLALWMAIGGTAGAALGLACVAAALAGGHR
                     LMIAPPGVPILTYHSVSPDPAWLPWSKEISVHPDTFERHLRTLRAMGCAVIPSLELVE
                     RRGSGRPLPANSVVLHFDDGYLDNWLFAVPLLRRYAMPAAFFASLDFIAPGEAVRDGG
                     ADCSGYMNWAELKAIDADSSFEVEAHGVDHARVAISERIVDKVTADNWRRLAWVQWAR
                     MEGPKHDWYRAEAPPAAPIGTLVPE"
BASE COUNT         1013 a         2237 c         2361 g          965 t
ORIGIN      
        1 cgcgagcccg ccgcagaaga tgagcggccg cacccgctcg tcgccatcgg ccgcgccggc
       61 cgggggaagc tcatgatgga gcgccgagat ggccgcgacg cgctcgccgc gcgcctggag
      121 gaacgccctg aggaggttga acaaggcggt ggcgtcggca tcctggccgg cgccgagcac
      181 gtagagcggg cggccgggct gcatcagcgg ctcggccagc cagctttcga gcttggcctt
      241 gatgtcgcgg ctctccttgg cgctgaagct cccgcccgac cgcggcgcct tcagcacgcc
      301 gggataggag cgcgggccgg cgtcggaccg gacggtggtg cgccggcggc ggaccgccgc
      361 gaccgtcgcc ggcgccggcg ccagcagcat gatcatgccg gcgagcgcgc agccggcgat
      421 gaagcccatc gccgtgttga ccatcacctt gggcgaatcg cgcaccagcg gcggcgtcgc
      481 gcgctccacc acccgtaggg tcgggccgac cgcgagccgg tcgagctgca cctggctcgc
      541 gcgttcggcg agcgcggtcg cttcgacctg gaggatggcg atgtcgcggt cgagcgcgct
      601 gatcgtggct tcggcccgcg ccacccgatc gagctggccg ttgaggccgg cgatgcgcgc
      661 ccggcgctgg gccgcggcct gggcgagccc cgccgcccgc ggccgctcca gcgcaagcgc
      721 ctggtcgacc gccggagtgg aggccgacaa tttaccacca gcctgacgct gcagcacccc
      781 gacctgcgcc gtactcgacg acaattgcgc catttcgccg gtgagccgcg cttcctcggc
      841 cagccgctcc tcgacgatcg cctggcgctg ggcgggaatg tcggcgacgc cgagcgagcc
      901 gcgggtcgcc accgcctgcc tttgcttgtc ctcgagcgtg gcgcgggtct gcgccagccg
      961 ctcggccagc tgacggttga ggcggtcggc gggcagctcc gtgcggaccg cggccgccgc
     1021 catcagccgg tcggtgatct cgttggcgaa ccatgcggcg agccggcgat tgtcccactc
     1081 gacgtcgatg cgcagcaaat aggtgccgtt cacggtcgcc acgcgggtcg cctcggtgag
     1141 cgcgtcgatc agcccctcgc gcgggttctg gaccggcacg ccgccataat tgagcgagtt
     1201 ccacatccgg cgcaggccga gcccggtctg cggctgcggc tggagcgcag ccggcacctt
     1261 ggcgatatat tcgtcgatga cggaggcgag gaccggccgc gacatcgcat attcggcata
     1321 ggtctgggtc agcacgcccg cgctttggag gccggtgttg cgtacgaact ggttggcata
     1381 ttcgagctcg gcgcgggacg gcgagacatc gagcgtcgcc gacgccttga aagtggggtc
     1441 gcgggcgagc acgagcgagc tgtagaggaa cgccgccagc gtggtcgcga cgacgatcgc
     1501 gaccaggatc ggccagcgcc gccggaacaa ggcccatagc cgcgccggcg cgagacccgt
     1561 ctccgcctgg ggggtcgcgg ccatcagcat aagcctcgag gcatgagcgc atgcatcagt
     1621 tgttctcgcc tccgaggccg atcctgaagc cggtgccgaa gccggtgaag ccgaggacgc
     1681 gtcgaatcgt ctcgctgcgg tcggcgctca gcaccagatt ggtcggcggc acgatgatca
     1741 ccgtataggg gctgatataa tcgagcgtgc gcgcgcggcc gtcgtcatgg ccgctgaaca
     1801 gcgcctggac atcgaccagc ctcgccagca tctgctcgcc gcgcggctcg accacgacca
     1861 cggtgctgag cttggcttga cggttcgacc cgccggcgcg cgcgatagcc tccaggaggg
     1921 tggtgccttc gcgataggcg atggcgccgg ggcggatgac ctcgccgagc acgaaatatt
     1981 cgggcggcgc cggcttctga ttatattcga ccatcgccag ctccaccgac ggtcgctcca
     2041 gcttcggctc gtaaagctgc tcaaggcgct gctggagctg ggcggcggtg aggccgcggg
     2101 tctggatcgg atcgagcagc ggcgggctga tgaagccgga aggctgaacg cgctgatcgg
     2161 aattcagggt cggcagatag gggaagctga ttcggacgag gtcgcccggg tggaaatcgc
     2221 cgcccgcgcc gagctgctcc tggatgcgca gattggcctg ctgcgccgcc agccgcgacc
     2281 cctgggtaag agcgatcggc gcgggaagat catagttcgt gctcgcaaag cacccggtaa
     2341 ggagcgccgc caatgcggat aacgcgaaca ggttcctcaa caacactcgc agccaccttc
     2401 cacatcggcg ccagcaaaac tcgcggacgc gccccgcatg gtatagcgga gaagaaattt
     2461 attccggtta aaccggtgaa gctacggcga ttttccttag gggagccggc ttgtgtcccc
     2521 gcgcgggacg gcgccggggc gcgcccgggc ggcgagatcg agcagcgcac gctcaaaaat
     2581 ccctgtggct tcaaagacct cgaaccggtt ggcggtggcc gccgccgccc ctatgaccga
     2641 ggctcggcgc tcgggcgaat taaccaatga ttccatcgct tcggccagcg cttcagtgtc
     2701 cccgggccgc accaggaagc cgttttcgcc atcggatatc aggctgcggg ggccgtcgca
     2761 ggcggtagcg agcaccggca cgcgggccgc catcgcctcg acgatgacgt tgccgaagcc
     2821 ctcgcggcgc gaggccagga cgaacaggtc ggcgcggccg agctccgcgc cgagatcgga
     2881 agcgtggccg gcgaggctcg ctttgccggc gaggttcaac tcgccgatcc gggcttggag
     2941 ggcggctcgc tcgggacctt ccccgaagat cgccagctcg aaatcgactc cgcgccggtc
     3001 gagcatggcg caggcgtcga gcaggaggtc gaaacccttg gccgggacca ggcggccggc
     3061 cgatatgatc ctcacctttc cggcggtccc ggggcgatcg cgcaggcgca ccgcgtccac
     3121 caccgggttg ggcaggacca atgtcggcaa gtcggcccgc cccagcacct tcccgagttc
     3181 ttcggctatg ccgggcgtgg gcacggcgat ggcgtcggcg cgcgggtaga gatggcgcat
     3241 caataaccgg cgcaaccccg gatgcttggc ctcggcgaga taggcgtcgg gggaggtgtg
     3301 ctcggagacg atccgcagcg cgttgccgcc gccgcgcccg atcgcgctcg ccagcagcgc
     3361 cagattcatg gcgttggtcg ccgaataagc gatgtcgatg ccggcggcgc cgagcgtgtg
     3421 gcgcagccgg cgcggcaggt ggcgcgcgcg ggcggcgccg agctcgaccg gctgcacgtc
     3481 gtccggcagc aaatcgagaa aggcgccctc ggcgtgggac aggaacagat gcggcgtgaa
     3541 gcggccgcga tcgagcgcgc gcaggaggtt gagcaccacc cgctccgcgc cgccgggccg
     3601 gaagtggggg aggagaaagg cgagccgcag cggcctcacg cggcgcggct tcgaagatgg
     3661 cgcagcgagc ggctgaggcc gcgatagatt tgccggccgt tgaagaagac gaggtgccgc
     3721 agcggtcgca gcggcgcgac gtgcgccggc accagccggt cgatctgggg ggcgaaggcg
     3781 cctttgaact ggtcgcggct cgcctcgccc tcatctccgc tctcgccctc cgacatcccg
     3841 gccagatcgt aggaccggca acccatcgtt cgggcgaggc ggatcgcttc ccacaacaaa
     3901 gcataagagc gcgggatcgc gggatcgaga tcggacgtgg ccatcgacag ccagatcgcg
     3961 tcggcgccct ggacgacgaa ggcgtggccg ccgatcaccg ccccctcgcg ctcggcaatc
     4021 aggatggcgc cgccattggc gcggacgatc gcggcctgct ggaggacgtc ggggaggccg
     4081 gccgtctcgt agcgcgattt gcgccgctgg aagtcgctaa gcaccgcctg gtaagcggcc
     4141 agatcctcgg ccgagacggc gggccgcacc gtcaccccct gcttggcggc tgcgcggatc
     4201 tgccgccgcc cgcgttgctt gaagcccgcc agcaccgctt cttccccgcg cccgaggtcg
     4261 atcttgccgg tggcggtgtg cagcgattgc cgatccgcgg gcagcggtct cagaccctgc
     4321 cccttgagga tgcggagagc ctccgcgcgc tcgtcgccgc cgatccgcgg ccccagcacc
     4381 agagtcgaga agcccgcctg ccgaagcgcg tgcttcagcg ccgcgacgac cgcgcccagc
     4441 cgctcgacat cgtgcaccac cggcccccgc tgcagcgtcg ccagccgcgc cagcggcccg
     4501 agccggctgc gccgcaccac cccggcgccg accagcgctt cgccgtcccg gcacaggaag
     4561 tagaggaagt cctgacgccg cgcgcgaggt gcatgctcgg cccaggcgcg gctctgctga
     4621 taggccgaat aagggctgcc gccgacgaag gcgtcgaagg cggcgcgatc ctcctccgac
     4681 agctccgccg ccagcaccgt tttgagacgt tcagggatca cgccgccgcc gctcttgctc
     4741 tttgcggccg cagcctagca gaaggaaccg cgccgatcgc gacaggagca tatgctggtt
     4801 ccatttcatc tctctgaaag ctggtttgac tgctggttcg ccgcttaccg caacaccccc
     4861 gccgcttatc tgccggtggc ggagggacgg ctttatcacg tccaggacaa gcgcaaagta
     4921 gggccgctca gtttcaaagt gacacgcggg gcgaccaatc tccagaccgc ctatttcgat
     4981 atttgcggaa ccggggacgc cgaggcggcc gcgggcgcgg cggcgcggct gatcgaggcg
     5041 accggcgccg acatgctgga attcgactat gtgctgaaag atgcgctgct ctaccgggcc
     5101 gcggagacct gggccggcag cggctggacc agcatcgagc cgctcgggcg gacggcgctg
     5161 gtcgattgca gcggccgctt cgacgattgg ctcgccgggc gccgcaaatc ggaccgccgc
     5221 gcctggctca agagcgagcg gcagatgccg gagcgggggc tcagctacga gctggtcacc
     5281 ggggcggccg aggccgagag cgcaatcccc gagctgctcg acgtcgaagc ttcggggtgg
     5341 aagggggcgg cggggagcgc gatccgccat aatccagagg acgcggcttt ctatgccagc
     5401 ttgacgcggc gggcggcgga acagggcgcg ctgcatctgg cgatggtccg ccacgaaggg
     5461 cgcgcggtcg ccttcgacta tggcattctc agcggcgacc gctgcctggc gctcaaggcc
     5521 tcctaccggg aggaatataa ggtgcagagc atcggtcacg tggccgcgat gaagcacgtc
     5581 cgcgacatgt tcgcccgcga cgggatccgc tattacgaca atatgggtaa cggcctcacc
     5641 ccgcagcggc acaaggtccg cttcgccact cattaccagc ttttctatcg gataagggtg
     5701 ttcgcgccga ctgcgcgcgg gctgctgctg cgcgaggcgt gccgggccta tcgcggcgcg
     5761 cgcgcggtgc gcgaccggat ccggcccaag ccgagcttcg agccgagcct gtgaccgcga
     5821 gccgcaagca ggatcgtcgg cgccgcgtga cccaatgggc gtcgatcaag ggggtcggcg
     5881 cggcggccgg gctagcgctc tggatggcga tcggcggcac ggccggcgcg gcgctcggct
     5941 tggcctgcgt tgcggccgcc ctcgccgggg ggcaccggct gatgatcgcg ccgccgggcg
     6001 tgccgatcct cacttatcat tctgtcagcc ccgacccggc ctggctgccc tggtcgaagg
     6061 agatttcggt ccatcccgac acgttcgagc ggcacctgcg cacgctccgc gcgatgggct
     6121 gcgcggtgat cccgtcgctg gagctggtcg agcggcgcgg cagcggccgg ccgcttccgg
     6181 ccaattcggt cgtcctccat ttcgacgacg gctatctcga caattggctg ttcgcggtgc
     6241 cgctcctccg ccgctacgcg atgccggccg ccttcttcgc ctcgctcgat ttcatcgcgc
     6301 cgggcgaggc ggtgcgggac ggcggcgcgg actgcagcgg ctatatgaat tgggccgaac
     6361 tcaaggcaat cgatgccgat tcttcgttcg aggtcgaggc gcatggcgtc gaccatgccc
     6421 gcgtcgcgat ctcggagcgc atcgtcgaca aggtgaccgc cgacaattgg cggcggctgg
     6481 cgtgggtgca atgggcgcgg atggaggggc cgaagcacga ttggtaccgg gccgaggcgc
     6541 cgcccgcggc cccgatcggc acgcttgttc cggaga
//
LOCUS       DASVRK010000202         6646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_349666, whole genome shotgun sequence.
ACCESSION   DASVRK010000202 DASVRK010000000
VERSION     DASVRK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349666"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF589_07905"
     CDS             complement(<1..183)
                     /locus_tag="VF589_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257543.1"
                     /translation="MKRPDVSGLTQPGDWSTACAAAATWPAADASAFFTSHFETARVG
                     PGKAFATGYFEPQIAGS"
     gene            complement(591..1238)
                     /locus_tag="VF589_07910"
     CDS             complement(591..1238)
                     /locus_tag="VF589_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEX8257544.1"
                     /translation="MTVEIVLLAMVALFVGLRLYAVLGRRTGHEQQPVTRPEAVGAAE
                     KAAPVTDLAPERTEGSGLVYQERAASGIRGIIAADPGFDVARFLEGAQAAYRMILEAF
                     WKGDREELTHLVGDEVRASFIGAIDEREAASHRLDNRLVAIERAQIEDAELNGRTASI
                     TVRFDADIAAVTRNEAGEVIAGSLTDAVDTHDVWTFRRTLGSSDPNWLLVETDEA"
     gene            1423..1944
                     /gene="secB"
                     /locus_tag="VF589_07915"
     CDS             1423..1944
                     /gene="secB"
                     /locus_tag="VF589_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX8257545.1"
                     /translation="MAEVRAAEDDSRPQVNGDDTAPQVGILSQYVKDLSFENPNAPAV
                     YQWQGQPQIEVQFNIGAQSVGQDMHEVALKIEVAARSESRTAFQVELVYAGLFALRNI
                     PEEQLQPFLLAEAPRIIFPFARRIVSETVQDGGFPPLLLDPIDFGALYMQRAAEAQAQ
                     ASGGQAGEITGQA"
     gene            1966..3540
                     /gene="murJ"
                     /locus_tag="VF589_07920"
     CDS             1966..3540
                     /gene="murJ"
                     /locus_tag="VF589_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX8257546.1"
                     /translation="MSLLKATSTIGGFTMVSRVAGFARDMIMARLLGAGLAADAFFLA
                     FQLPNIFRRLFAEGAFSAAYVPMFSRRLHAEGGRESAEEFSSDVLSVFVPVLIVFAAL
                     FELAMPGVIWLMASEYEDVDGKFELTVSLARITFPYIILISLVSLLTGILNSLSRFAP
                     GASFPIFLNLVLIAALFVGDALRGGTGNDEILAYTLAVAVSIGGVIQLLWLWFWTRRE
                     GVRLHIRRPRLTPEVRELGIIILPAVFGAGVYQINQLVQLFFVTRLEDGALSYLNYAD
                     RLNQLPLGIIGVALGTAILPTLSRFIGGGNKEGADRIQSDAIELAMLLTLPAAAALAV
                     CAQPFVTAFFLGGRFDAGDVAITARVITALVAGLPAYVLIKVLTPGYFARKDTKTPVY
                     AALAALAVFVVFNLLFIERFGIVGLAAATAVGAWLNSLLLYLILVRRGHYRIDARLGA
                     RLLRQLVAAAAMVAVLWLLRDALAGAFSGGILERVLAIGALVGSGSLVYFGIAFAIGA
                     VDRERLVMFKRRKAPQ"
     gene            3540..4538
                     /gene="trpS"
                     /locus_tag="VF589_07925"
     CDS             3540..4538
                     /gene="trpS"
                     /locus_tag="VF589_07925"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX8257547.1"
                     /translation="MRVVSGIQPTGNLHLGNYLGAIQRWVKMQDEADCFIFLADLHAI
                     TVYIDPAELAGNVREMAAALIACGVDPDKVVLFNHARVPAHSELAWILNCTARIGWLN
                     RMTQFKEKSGKNREGASVGLFAYPVLQAADVLLYQATHVPVGEDQKQHIELIRDIATK
                     FNTDYGTELFTRPDPLIGAHAARIMSLRDGGAKMSKSDPSDMSRINLTDDSDTVGQKV
                     RKAKTDPEPLPETMEELGARPEARNLLAIYAALEGSEPAEVLGRFAGQGFGAFKPALA
                     ELMVETLRPIKAKLDGLREDPAELDSILERGAERAAAAAKPTLDGAYRAVGLRRGA"
     gene            4663..5322
                     /locus_tag="VF589_07930"
     CDS             4663..5322
                     /locus_tag="VF589_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX8257548.1"
                     /translation="MSLSKNLMTLVAPVALLALSACATGFSTQVSRFQAMPAPQGQSF
                     YIQAERPENRGGLEFGRYADLVRRHLVGQGFTEAASPGTATLAVTLDYGVDNGQTKVV
                     STPGFGGYGGFYDPFYPRFYYGRRHPFFYGWRDPMWDSALAYPDVHSYTVFTSYLDMD
                     IRRTADGQSLFEGTAKARSRTDQLQVLVPNLVEAMFTGFPGSSGETVKITVPPPGHQR
                     G"
     gene            5461..5613
                     /locus_tag="VF589_07935"
     CDS             5461..5613
                     /locus_tag="VF589_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257549.1"
                     /translation="MANTLPRRFVMVAAPSVHAGIGDALRKAFHRPDLWSIKMFEKLI
                     GTLDRK"
     gene            complement(5731..>6646)
                     /gene="dnaA"
                     /locus_tag="VF589_07940"
     CDS             complement(5731..>6646)
                     /gene="dnaA"
                     /locus_tag="VF589_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX8257550.1"
                     /translation="FNPLFLHGGTGRGKTHLLHAIGHEFRARRPGAKIVYMSAEKFMV
                     EFVAALRANETIRFKQQLRSADLLMIDDVQFIAGKESTQEEFFHTMNEIISAGRRLVI
                     SSDRAPQDLDGIEPRILSRLSWGLVADINQADLELRLNIIIKKLEALPDVKVPHEVMM
                     FLAKRISSNVRELEGALNRIAAYAMMSGRPIDLDFVGEVLANILRANQRRISIDEIQS
                     RVSDHYRIRKAEMTSARRAREVARPRQVAMYLSKQLTPRSLPEIGRRFGGRDHTTVIH
                     AVRQIEKLRAQDAELDADIRLLTRQLEN"
BASE COUNT         1045 a         2266 c         2210 g         1125 t
ORIGIN      
        1 cgagccggcg atctgcggct cgaaatatcc ggtggcgaag gccttgcccg gccccacccg
       61 cgcggtctcg aaatggctcg tgaagaaggc ggacgcatcg gcagccggcc aggtcgcggc
      121 cgcggcgcag gcagtcgacc agtcgccggg ctgggtgagg ccggaaacgt ccgggcgctt
      181 catcagcgag ggacagctta ttcggaaagc ggcgagcgcg gatcgagccg ctgcctcggt
      241 gaccccgagc cgcgccaacg aaggccccgg cctgaccccg gccgatacgg cgtcggcaac
      301 cgggggcggg gctggcggga gcggccgctg cggcggcggc ggcgggcgat cgggcgggat
      361 cagccgcggg gcgcagctcg ccagcgcgac caaggcggca tagaggcagg cggagcgaag
      421 cgagaaccgg gcaacgccgt cggcatcgtc gccgcgccgc gcgccgcccg tgggcgcgcc
      481 cctgatcccc ctactcgaca ccccgccact ataggcgacg aagctcgcca agggaaaggg
      541 gcgagccctg gggcacgaaa gcggcgacag cggccgccgc ggcggcggga tcaggcttcg
      601 tcggtctcga ccagcagcca gttcgggtcg gaactgccga gggtgcggcg gaaggtccag
      661 acgtcgtggg tgtcgacggc gtcggtcagc gagccggcga tcacttcgcc cgcctcgttg
      721 cgggtgacgg cggcgatatc ggcatcgaag cggaccgtga tgctggcggt gcggccgttc
      781 agttcggcgt cctcgatctg ggcgcgctcg atcgccacca gtcggttgtc gaggcggtga
      841 ctggcggctt cgcgctcgtc gatcgcgccg atgaaggagg cgcgcacctc gtcgccgacc
      901 agatgcgtca gctcctcgcg gtcgcccttc cagaaggctt cgaggatcat gcgataggcg
      961 gcctgggcgc cttcgaggaa gcgggcgacg tcgaagccgg gatcggcggc aatgatgccg
     1021 cggatgccgg aggcggcgcg ctcctgatag acgaggccgc tgccttcggt gcgctcgggc
     1081 gcgagatcgg tgacgggcgc ggccttttcg gcggcaccga cggcttcggg gcgggtcacc
     1141 ggctgctgct cgtgcccggt gcggcggccc agcacggcgt agagccgcag gccgacgaac
     1201 agggcgacca tcgccagcag gacaatttca accgtcaccc attctactcc gcacagggat
     1261 cgaaactcct acataggctt agtgcgccga tgattcaaat gcgcagcggg gcgccggtgc
     1321 cggcgcgcgg caggttgcgc gtccgggcgg ctcctgctac ccgcaggcgc gttccggccg
     1381 acgcccggcc gagcccctcc aaacttcctc gaaggtattg aaatggccga agttagagcc
     1441 gccgaagacg atagccggcc gcaggtcaat ggcgacgaca ccgcccccca ggtcgggatt
     1501 ctctcgcaat atgtgaagga cctttcgttc gagaatccga acgcgcccgc cgtctaccaa
     1561 tggcagggcc agccgcagat cgaagtccag ttcaacatcg gcgcccagtc ggtcggccag
     1621 gacatgcacg aggtcgcgct gaagatcgag gtcgccgcgc gttccgaaag ccgcaccgcc
     1681 ttccaggtcg aactcgtcta tgccggcctg tttgcgctcc gcaacatccc cgaagagcag
     1741 ctccagccct tcctgctggc cgaagcgccg cggatcatct tccccttcgc ccgccgcatt
     1801 gtctccgaaa ccgtgcagga cggcggcttc ccgccgctgc tgctcgaccc gatcgacttt
     1861 ggcgcgctct acatgcagcg cgccgccgag gcgcaggcgc aggccagcgg cggccaggcc
     1921 ggagagatta ccggacaagc ctgagcgtaa cgggggggtc cgcgcatgag cctgctcaag
     1981 gccaccagca cgatcggcgg cttcacgatg gtgagccggg tcgcgggctt tgcgcgtgac
     2041 atgatcatgg cgcggctgct gggcgcggga ctcgccgccg acgccttctt cctggctttc
     2101 cagctaccca acatcttccg gcggctgttc gcggaaggcg ccttttcggc cgcctacgtg
     2161 ccgatgttca gccgccgcct ccacgccgag ggcgggcggg aatcggccga agagttcagc
     2221 agcgacgtgc tctcggtgtt cgtgccggtg ctgatagtgt tcgcggccct gttcgagctc
     2281 gcaatgccgg gcgtgatctg gctgatggcc tcggaatatg aggacgttga cggtaagttc
     2341 gagctcaccg tttcactggc gcggatcacc ttcccctaca tcatcttgat cagcctcgtc
     2401 tcgctgctca ccggcatcct caactcgctt tcgcgtttcg cccccggcgc ctcctttccg
     2461 atcttcctca atctggtgct gatcgccgct ttgttcgtgg gcgatgcgct tcgcggcggc
     2521 accggcaatg acgagatcct cgcttatacg ctggcagtcg ccgtctcgat cgggggcgtc
     2581 attcagctgc tctggctgtg gttctggacc cggcgcgagg gcgtgcggct ccacattcgc
     2641 cgtccccggc tgacgccgga ggtgcgcgag ctcggcatca tcatcctgcc cgccgtgttc
     2701 ggcgccggcg tctaccagat caaccagctc gtccaattgt tcttcgtgac ccggctcgag
     2761 gacggcgcgc tcagctacct caattatgcc gaccggctga accagctccc gctcggcatc
     2821 atcggcgtgg cgctcggcac ggcgatcctc ccgacgctgt cgcgcttcat cggcggcggc
     2881 aacaaggaag gcgccgaccg gatccagtcg gacgcgatcg agctcgcgat gctgctcacc
     2941 cttcccgccg cggccgctct ggcggtgtgc gcccagccgt tcgtcaccgc ctttttcctc
     3001 ggcggccgct tcgacgccgg cgacgtcgcc atcaccgcgc gcgtgatcac cgcgctggtc
     3061 gcggggcttc ccgcttatgt gctgatcaag gtgctcaccc ccggctattt cgcgcgcaag
     3121 gacaccaaga cgccggtcta tgcggcgctg gcggcgctgg ccgtcttcgt ggttttcaac
     3181 ctgctcttca tcgagcgttt cggcatcgtc ggcctcgccg ccgcgaccgc ggtcggcgca
     3241 tggctcaaca gcctgctcct ctacctgatc ctggtccggc gcggccatta ccggatcgat
     3301 gcccggctcg gagcgcgcct gctccgccag ttggtcgccg ccgcggcgat ggtggcggtg
     3361 ctgtggctgc tgcgcgatgc cctggccggc gccttttccg ggggcatcct ggagcgcgtg
     3421 ctggcgatcg gtgcgctggt cgggtccggc tcgctggtct atttcgggat cgccttcgcg
     3481 atcggcgcgg tggacaggga aaggctggtc atgttcaagc gccgcaaggc tccgcaatga
     3541 tgcgcgtcgt ctcgggcatc cagccgaccg gcaacctcca cctcggcaat tatctcggcg
     3601 ccatccagcg ctgggtgaag atgcaggacg aggccgattg cttcatcttc ctggctgacc
     3661 tccacgccat caccgtctat atcgacccgg ccgagcttgc cggcaacgtc cgcgagatgg
     3721 cggccgcgct gatcgcctgc ggggtcgacc cggacaaggt cgtgctgttc aaccacgccc
     3781 gcgtccccgc ccattccgag ctcgcctgga tcctgaactg caccgcccgc atcggctggc
     3841 tcaaccgcat gactcagttc aaggagaaat cgggcaagaa ccgcgaaggc gccagcgtcg
     3901 gcctgttcgc ctatccggtg ctgcaggcgg ccgacgtgct gctctaccaa gccacccacg
     3961 tccccgtcgg cgaggaccag aagcagcata tcgagctgat ccgcgacatc gcgaccaagt
     4021 tcaacaccga ctatggcacc gagctgttca cccggcccga cccgctgatc ggcgcgcatg
     4081 cggccaggat catgtcgctc cgcgacggcg gcgccaagat gtccaagtcc gacccgtccg
     4141 acatgagccg gatcaacctc accgacgaca gcgacacggt cggccagaag gtgcgcaagg
     4201 cgaaaaccga tccggagccg ctgcccgaga ctatggagga actgggtgct cggccggagg
     4261 cacggaatct cctcgccatc tatgccgcgc tcgaaggcag tgagccggcc gaggtgctcg
     4321 gacgcttcgc cggccagggt ttcggcgcgt tcaagccggc gctggcggag ctgatggtcg
     4381 agacgctgcg gccgatcaaa gccaagctcg atgggctgcg cgaggatccg gcggagctcg
     4441 actcgatcct cgagcgaggc gccgagcgcg cggccgcggc cgccaagccg acgctcgacg
     4501 gcgcctatcg tgcggtgggg ctccgcaggg gggcgtaagc gcccgcagcc gtcggagagc
     4561 cgggactcgg ctcgtcgttc ttcagcaacc gttcagcgat tggcgctaag ctgaagttcc
     4621 tgtatatcct tcacattccc gatcccgaga aaggtcgcaa acatgtccct ctcgaagaac
     4681 ctcatgacgc tcgtcgcgcc ggtcgcgctg ctcgcgctga gcgcctgcgc caccggcttt
     4741 tcgacccagg tgtcgcgctt tcaggcgatg ccggcgccgc agggccagag cttctatatc
     4801 caggccgagc gccccgaaaa tcgcggcggc ctcgaattcg gccgctatgc cgacctggtg
     4861 cgccgccatc tcgtcggcca gggcttcacg gaagcggcct cgcccgggac ggcgacgctg
     4921 gcggtaacgc tcgattatgg cgtcgacaat ggtcagacca aggtcgtgag cacgccgggc
     4981 ttcggcggct atggcggctt ttacgatccc ttctatccgc gcttctacta cggccgccgc
     5041 catcccttct tctacggctg gcgcgatccg atgtgggatt ccgccctcgc ctacccggac
     5101 gtgcacagct acaccgtctt caccagctat ctcgacatgg acatccgccg caccgccgac
     5161 ggccagtcgc tgttcgaagg caccgccaag gcgcgctcgc ggaccgacca gctgcaggtc
     5221 ctggtcccga acctggtcga ggcgatgttc accggcttcc ccggcagttc cggcgagacg
     5281 gtcaagatca cggtcccgcc gcccggccac cagcgcggct aagccacgga gcagattgca
     5341 ggaatcggcg cggcgggcgc gtcccgccgc cgtccgccgc tccggccgca cgtcccatcg
     5401 ccgccgcgca acctaatgta aatcaacgtg ttatgtgatg aacaagctga ggagtgagca
     5461 atggcaaaca cacttccccg gcgctttgtc atggtggctg cgccgagcgt acacgccggc
     5521 attggcgacg cgcttcgtaa agcgttccat cggcccgacc tgtggtcgat caagatgttc
     5581 gagaagctga tcggcacgct cgaccggaaa tgatcccctc cagggggccg acgcaaaaag
     5641 gggagagacg ggccaccgtc tctccccttt ccgtactggg cctacatcgc ccctggcaaa
     5701 tgggcctaca tcgcccggat taacagccgc ctaattttcc agctgacggg tcagcagccg
     5761 gatgtcggcg tcgagttccg cgtcctgcgc gcgcaacttc tcgatctgcc ggacggcgtg
     5821 gatgacggta gtatggtcgc gcccgccgaa ccgccggccg atctccggca gcgagcgtgg
     5881 cgtgagctgc ttcgacagat acatggcgac ctggcgcggg cgcgcgacct cgcgggcgcg
     5941 gcgggccgag gtcatctccg ccttgcggat gcggtaatgg tccgacaccc ggctctggat
     6001 ctcgtcgatt gaaatgcggc gctggttggc gcgaaggatg ttggccagca cctcgccgac
     6061 gaagtcgagg tcgatcggcc gaccgctcat catcgcataa gcggcgatcc ggttgagcgc
     6121 gccttcgagc tcgcggacgt tggagctgat ccgcttggcg aggaacatca tgacctcgtg
     6181 cggcaccttc acgtcgggca gcgcttcgag cttcttgatg atgatgttga gccgcaattc
     6241 gagatcggcc tggttgatgt ccgccaccag gccccaggag aggcgcgaga ggatgcgcgg
     6301 ctcgatgccg tcgagatcct gcggcgcgcg gtccgacgag atcaccagcc gccgcccggc
     6361 cgagatgatc tcgttcatgg tgtggaagaa ttcctcctgg gtcgattcct tgccggcgat
     6421 gaactggacg tcgtcgatca tcaggagatc ggccgagcgc agctgctgct tgaaccggat
     6481 cgtctcgttg gcgcgcaagg cggcgacgaa ctcgaccatg aacttctcgg ccgacatgta
     6541 gacgatcttg gcgccgggcc tgcgggcgcg aaattcgtgt ccgatcgcgt gcagcaggtg
     6601 ggtcttgccg cggccggtgc cgccgtggag gaaaagcggg ttgaac
//
LOCUS       DASVRK010000203        14560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_37262, whole genome shotgun sequence.
ACCESSION   DASVRK010000203 DASVRK010000000
VERSION     DASVRK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 14560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14560
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37262"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VF589_07945"
     CDS             complement(<1..1452)
                     /locus_tag="VF589_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HEX8257551.1"
                     /translation="MKQGLAGLVSMLLIGAAPAAAAPASAAPANAAPANAAEALRGTA
                     LKSGLLPVHVDNKQGRILLSLPAPDAEGISGRYLYVTSLRTGLGSAPIGLDRALSAGS
                     KLLAFRRIGKKMVAEIENPRFRAAGAPAPEQAAARESFAYSTIWMGDIAAETNDGRLL
                     VDVSGLLTRDAMGIAQALKGGGEKDYKLVPELTVADPGSVKVFPENIEMEARQTFVSA
                     EPGAEARNIAPAQGILSFIVRHSLVKLPAPGYTPRQFDPRTGGFATQILDYAAPLGQS
                     IVQELANRFRLEKIDPAAPRSRVRKPIVFHIDRSAPEPIRTALLEGTGWWKRAFEEAG
                     YIEAFEVRMLPEGADPLDVRYNVVNWVNRATRGWSYGQVIEDPRTGEIVKSQVLLGSL
                     RVRQDMLIFEALVGADRVGTGGPNDPVVAALARLRQLAAHEVGHALGLAHNFAASSQG
                     RFSVMDYPAPRVTVSNGAPDLSDAYGVGLGRWDM"
     gene            1634..3778
                     /locus_tag="VF589_07950"
     CDS             1634..3778
                     /locus_tag="VF589_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8257552.1"
                     /translation="MKAFALFLTTTALAALPAAARAADAPVQGTDHVHTEEPEEIVVT
                     ANFIRDLDLLAGTEVLTGTELVRDLRPQLGDTLARLPGVSATSFSPGASRPVLRGFQG
                     ERIRVLTDGVGSIDVSNTSADHAVTIDPLTAERIEVLHGPAVLLFGGQAIGGAVNVLD
                     RRIPRRVPDEVIHIDALASYGSAADEQSVGGSIDIPLGGGFVGHVDGSYRNTDDLKVG
                     GFVLSPRLRAEQLAVAAEEQAEGHAEEAAEARELANLRGRIPNSATETKTLGAGLALI
                     GDRGSLGFSLSWYDSNYGVPARPGAEHHHEKPEGEHEEAEQGGHDEHGEAPVTIGLEQ
                     FRGDVRGEFEVGGGFVDKLRLRLAAADYEHTEFEGEEVGTIFRTQGMEGRFELVQADR
                     AGWRGVSGVQYFARDFAAVGAEAFVPPNETSQLGLFTLQEFDLGKAGIEAAGRWERTR
                     VGSNEVGVARTFNAYSGAFGGSYAVTPRIKIGANVSRSERAPAAEELFSNGPHIATQA
                     FEIGDPDLQKEKSWGAEAYVRADTRDFNLSLSAFANWFDDYIFEAATGEEQDELPVFR
                     YFQRDARYYGFEGEISATLFRAGGFRFVGDVVADYVHASIKEGGPVPRIPPLRVLAGI
                     EAQSESIDGRIEAEWSADQDRVAAFETPTDGHLLVGASVAWRPWGKRNPTTFLVSANN
                     IFDVDARRHASFTKDYVPLAGRDIRLSARFSF"
     gene            complement(3768..4763)
                     /locus_tag="VF589_07955"
     CDS             complement(3768..4763)
                     /locus_tag="VF589_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8257553.1"
                     /translation="MSKHDKSATYSRASDAGASDRRLWTWGAGAAGAALAATAVVNHR
                     RARRAERDNPPLGQFVTVDGVRLHYVERGEGEPLLLLHGNGAMIEDWEASGLLDELAG
                     SHRVIAIDRPGFGHSDRPRTTIWTPAAQAKLIAAALPRIGVKRPLVVGHSFGTMVALA
                     LALDHPSLVNGLVLLGGYYYPSARADVLFASQPAVPVLGDVMRYTISPLLGAALQPKI
                     RQKIFAPAPVPDKFELFPMEMALRPSQIRATAAEAALMVPAAAALAGRYADLGLPLTL
                     AAGRGDRLVDPLAQSARLHEAVAGSRLELIEGSGHMVHYTAMPRVAELIRDAAKS"
     gene            5021..5314
                     /locus_tag="VF589_07960"
     CDS             5021..5314
                     /locus_tag="VF589_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257554.1"
                     /translation="MIMTQARGRMAALALIALSFGAAASPVQARQSDAQIRQSIIRQS
                     IANYSGNCPCPYNTMRNGRRCGGNSAYSRPGGESPKCFADDVSQSEVNAYRRR"
     gene            5392..5760
                     /locus_tag="VF589_07965"
     CDS             5392..5760
                     /locus_tag="VF589_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8257555.1"
                     /translation="MKIEGGCHCKAVRFEAEVAEQVEVLDCNCSICAMTGFRHLMVPH
                     ERFRLLAGQGALTSYRFGTGAADHLFCGICGVKSFYQPRSHPEAWSVNLNALDDSGSL
                     RISVKAFDGRNWEAARAALD"
     gene            5802..6872
                     /gene="alr"
                     /locus_tag="VF589_07970"
     CDS             5802..6872
                     /gene="alr"
                     /locus_tag="VF589_07970"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8257556.1"
                     /translation="MGAAIGSAVNPPHRPLRLTIDSAALVSNWRWLARMSAGAACGAA
                     VKADGYGVGAAEVARHLASAGCRDFFVATWAEAEAVMPALAGESLSVLHGVGEDDVAY
                     AAASPARPVVNTPLQAKRWREHGQGRPCDVMIDTGMNRLGLSAEQVAEGALDGLAVDT
                     LMSHLACADEDVAANARQRGAFLKVAAKVAAKRLSLANSAGICLGPDYAFDLTRPGLA
                     LYGGIPRAEAEGHIRQVVRVEAEIVQRRRIRSGDSVGYNATFTADGDCELAILNLGYA
                     DGYLRDFSGTGRARIGGGFAPVVGRVSMDLTAIRVDAAPELREGDWVELDFDLPAASA
                     QSGLSQYELLTTLGARYQRLWS"
     gene            6869..7666
                     /locus_tag="VF589_07975"
     CDS             6869..7666
                     /locus_tag="VF589_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8257557.1"
                     /translation="MKAGVLAPVIAAFAAIGATVIGLFATVGRVALFAWTTVIRALIP
                     PYYPLRLLEQLLHIGWYSLPVVGLTAVFTGAALAQQIYVGGSRFNAQSTVPAIVVIGI
                     VRELGPVLVGLMIAGRVSSAMAAEIGTMRVTEQLDAMATLSTDPYRFLIAPRLFASVI
                     ALPLLVLVANAIGVLGGFILGVYKLGFNATAYLETSRRYMQEDDLVMSMVKAAVFGFF
                     IALMGCYHGFRSAGGAAGVGKATTNAVVSAFILILLANLLITVAAFG"
     gene            7663..8421
                     /locus_tag="VF589_07980"
     CDS             7663..8421
                     /locus_tag="VF589_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8257558.1"
                     /translation="MSAPPKIAIRGLAKRFDDNVVFDGVDLDVQPAESIGVIGQSGMG
                     KSVLIKCILGLIEPDAGSILIDGEEMVGAGERKWDKCRAKFGMLFQGSALFDSLPVWR
                     NVTFGLTQGRLQNPARMRAIAEENLERVGLGRDVLYLRPSELSGGMQKRVALARAIAP
                     RPEIIFFDEPTTGLDPIRADVINDLIVSLVEDLGVTALTITHDMASARKVAHRLAMLH
                     GGRIIWQGSRDRLYDSGNPFVDQFVQGRAEGPIH"
     gene            8470..9189
                     /locus_tag="VF589_07985"
     CDS             8470..9189
                     /locus_tag="VF589_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8257559.1"
                     /translation="MIEAHLSKLRARDELSEEEERAIRGAVSEVREFPADKTIIRAEQ
                     ELSVSTLLLDGLMCRYKDLRDGQRQITELHVAGDFADLHSMTLKRLDHNVMTLTPCRA
                     AIVPHDRLKAITEQHPHLTRVYWFSTNMDAAIHREWAVSLGRRAALSRVAHLFCELYV
                     RLGIVGLADDTGYALGLTQTDLAECLGLTSVHINRTLKELRERGLVEFRGGRVTIHDR
                     AGLERVAEFNPSYLYLERRRR"
     gene            complement(9212..10807)
                     /locus_tag="VF589_07990"
     CDS             complement(9212..10807)
                     /locus_tag="VF589_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8257560.1"
                     /translation="MLEFLDIFSRARDLAPHGYCLLWDPALVWTHVLADALIAAAYFS
                     IPVAIVRFVRGRSDLEFSWIFWLFALFILACGTTHLMAIWTLWNPDYGWEALIKVVTA
                     IASVGTAIVLWPLIPKALAIPSPLQLKEANVQLSERVRERDDALDRLKREIQDRERAE
                     AALVQAQKMEAVGQLTGGIAHDFNNLLTVVLGNLDMIRRSTAEPRTGRLVDSALQAAA
                     RGAKLTGQLLAFSRTQRLELRPVLVAPLIRGMRELLSHTLGPGVALELALDDAEVPVM
                     ADANQLELAILNMAINARDAMPGGGTLTIAARPRQLGDDPDLPDGEYLHLTVSDTGAG
                     MDAETAARAFDPFFTTKAVGQGTGLGLSMVYGVARQSGGIARIQSEPGAGTTLSILLP
                     RTGEAAIEPAQPDQASGPAGDGAAARILVVDDDADVRQVLVDTLAELGHRTSAASSGQ
                     EGLDLLQEESFDLLVLDFAMPGMNGAEVARVVRQARPEQKILFVTGYADTAIEAAAGD
                     APVLRKPFRQEDRAAAVNRALAG"
     gene            complement(10904..12247)
                     /gene="rimO"
                     /locus_tag="VF589_07995"
     CDS             complement(10904..12247)
                     /gene="rimO"
                     /locus_tag="VF589_07995"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX8257561.1"
                     /translation="MVMTLPSPPKVGMVSLGCPKALVDSERILTKLRSDGYQLSPDYD
                     AADVVLVNTCGFLDSAKEESLAAIGEAIAENGRVIVTGCMGREAEVIRARFPEVLAVT
                     GPHQYEQVVGAVHEAAPMPPSAFLNLVPDGGLKLTPRHYSYLKISEGCNHRCSFCIIP
                     SIRGDLASRRPDAILREAEKLIAAGTRELLVISQDTSAYGVDLRHAEWNWKGAPVRAH
                     MTDLARELGKLGAWVRLHYVYPYPHVDRVIPLMAEELVLPYLDIPFQHAAPSVLKAMK
                     RPANEAKVLERIHAWRAICPDIAIRSSFVVGFPGETEADFQYLLDWLDEARLDRVGAF
                     RFEPVEGAAANALPGAVPEEVKEERYARLMERTAAISAAKLQAKVGRNIDVIVDLADE
                     EGGATGRSKADAPEIDGEVHLRDAHGARPGDILRVLVEDADEHDLFGVPVSPAAS"
     gene            12473..>14560
                     /locus_tag="VF589_08000"
     CDS             12473..>14560
                     /locus_tag="VF589_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8257562.1"
                     /translation="MALGSNSINQVELSGNPDIDALLTGHRWANVVVTYSFPTSQGEY
                     SYHAITGFESFNVEQRAATDQIVANLNAVCGLNIVPGGPGSNIRLAEATTIDYTQVDA
                     EPAHGPGGENDSAEASPPQPDASYRVGDTWYNHSDYNNPVAGTFAYAGGLMHEIGHAV
                     GLAHGHETDEIEHSHGVHPKPALPRDHDTQEYSIMTYRQYVGEADPNDGNNGRDHAQS
                     LMINDIAALQYLYGAYYGHRSNNSVYSWSPTTGEMSIDGVGQGVPWIVELADGTLKST
                     VFRTIWDGGGIDTYDFSNYRTDLLVDLNPGGWSTISEDQLALLGNDGMRDQFARGNSA
                     NALLHQGNLQSIIENATGGSGNDRLVGNVVGNLLLGNPGRDTLFGNEGNDRLNGGTGA
                     DALIGGDGIDQASYVGAFVDGVTADLIFATANSGDAEGDSYDSIENLQGTSFDDDLRG
                     HDGENVLEGGFGGDDQLYGRAGNDLLIGADGDDLLEGGAGADALDGGGGNDTASYLFA
                     DSRILADLLFDFRFTNEGDALGDTYVGVENLIGSRFDDDLRGDDAANILSGRGGTDGF
                     NGRGGYDTLIGGRGSDLFFLQDLAYAGQFAGFQYDRVVETPDGGVDTVFVTAFDNPDD
                     FSTSGYTLGDNVEYGQIRGSVAFNLTGNALDNVLTGHGAANRLTGHAGSDYISGNGGI
                     DILIGGAGDDTYVL"
BASE COUNT         2330 a         4943 c         4994 g         2293 t
ORIGIN      
        1 catgtcccag cggccgagcc cgaccccata agcgtccgac agatcggggg cgccgttgga
       61 aaccgtcacc cgcggcgccg gataatccat caccgagaag cggccctggc tgctcgccgc
      121 gaaattgtgg gcgaggccga gcgcgtgccc gacctcgtgc gcggcgagct ggcgcagccg
      181 cgccagcgcc gcgacgaccg gatcgttggg cccgcccgtg ccgaccctgt cggccccgac
      241 caaagcctcg aagatcagca tgtcctggcg gacccgcagc gagccgagca ggacctggct
      301 cttgacgatc tcgccggtgc ggggatcctc gatcacctgc ccgtaggacc agccgcgagt
      361 cgcccggttg acccagttga cgacattgta gcggacgtcg agcgggtccg cgccctcggg
      421 cagcatgcgg acttcaaacg cctcgatata gcccgcctcc tcgaacgccc gcttccacca
      481 gccggtcccc tccaacaagg cggtgcggat cggctcaggc gccgagcggt cgatatggaa
      541 gacgatcggc ttcctgaccc gcgagcgcgg cgcggcggga tcgattttct ccagccggaa
      601 gcggttggcc agctcctgga cgatcgactg accgagcggc gcggcataat cgagtatctg
      661 ggtcgcgaag ccgccggtgc gcgggtcgaa ttgccggggc gtgtagccgg gcgccggcag
      721 cttcaccagc gagtggcgga cgatgaagct caatatgccc tgcgcgggcg cgatgttcct
      781 ggcctcggcg ccgggctcgg ccgacacgaa agtctggcgc gcctccatct cgatattctc
      841 gggaaacacc ttgaccgagc cggggtcggc gacggtgagc tccggcacca gcttatagtc
      901 tttctcgccg ccgcctttca gcgcctgcgc gatccccatc gcgtcgcggg tgagcagccc
      961 cgacacgtcg acgagcagcc ggccgtcatt ggtctcggcg gcgatgtcgc ccatccagat
     1021 cgtcgaataa gcaaaggatt cgcgcgcggc ggcctgctcc ggcgccggcg cgccggcggc
     1081 gcggaagcgg ggattctcga tctcggccac catcttcttg ccgatccggc ggaaggcgag
     1141 cagcttcgat ccggcgctca gcgcgcggtc gaggccgatc ggcgccgagc cgagcccggt
     1201 ccggagcgag gtgacataga ggtagcgccc cgaaatgccc tcggcgtcgg gcgcgggcag
     1261 cgacagcagg atgcgcccct gcttgttgtc gacatggacc ggcagcagac ccgatttgag
     1321 cgccgtcccg cgcagcgcct cggccgcgtt cgccggagcc gcgttcgccg gagccgcgga
     1381 ggccggcgcg gcggcggcag gcgcggcgcc gatcagtagc atcgagacca gcccggcaag
     1441 accctgtttc attcgcgaaa tcccttccgc gcatcgccta gcgcagccgc ggcgaagctg
     1501 tcgagcgcgg taatggccgc agctaggcga cgagccgtgg ctgccaagcg accggagcgg
     1561 agccgcgtaa acacacgacc ttgatacggt gtaacatccc gtctaccgcg ctcgctcccc
     1621 ctcggagttc ctgatgaaag ctttcgcgtt gtttctgacg accaccgccc ttgccgcgct
     1681 gcccgccgcg gcgcgcgcgg ccgacgcccc cgtacagggc accgaccatg tccataccga
     1741 ggagccggag gagatcgtcg tcacggcgaa cttcatccgc gacctcgatt tgctcgccgg
     1801 caccgaagtc ctcaccggca ccgagctggt aagggacctg cgtccccagc tcggcgacac
     1861 gctggcgcgg cttcccggcg tgtcggcgac gtcgttcagc cccggcgcgt cgcggccggt
     1921 gctgcgcggc ttccagggcg agcgcatccg ggtgctgacc gacggcgtcg gctcgatcga
     1981 cgtgtcgaac acgtcggcgg atcatgcggt gacgatcgat cccctcacgg ccgagcggat
     2041 cgaagtcctt cacggtcccg ccgttctgct gttcggcggc caggcgatcg gcggcgccgt
     2101 caacgtcctc gaccggcgca tcccgcgccg cgtccccgac gaggtgatcc acatcgatgc
     2161 gctcgcctcc tacggctcgg cagccgacga gcagagcgtc ggcggatcga tcgacattcc
     2221 gctcggcggc ggcttcgtcg gccatgtcga cggcagctat cgcaacaccg acgatctcaa
     2281 ggtcggcggc ttcgtgctgt cgccgcggct tcgcgccgag cagctcgcag tcgccgccga
     2341 ggagcaagcg gaaggccatg ccgaggaggc ggccgaggcg cgcgaactcg cgaacttgcg
     2401 cgggcgcatc cccaacagcg cgaccgagac caagacgctc ggggcgggat tggccctgat
     2461 cggcgatcgc gggagcctcg gcttttcgct gagctggtac gacagcaatt atggcgtccc
     2521 cgcccgcccg ggcgccgagc accatcatga gaagccggag ggcgagcacg aggaagcgga
     2581 gcaaggcggc catgacgagc atggcgaggc gccggtcacg atcgggctcg aacagtttcg
     2641 cggcgacgtt cggggcgagt tcgaggtcgg cggcggcttc gtcgacaagc ttcgcctgag
     2701 gctcgccgcg gccgattacg agcataccga atttgagggc gaggaagtcg gcaccatctt
     2761 ccgcacccag ggcatggaag gccggttcga gctggtccag gcggaccggg ccggctggcg
     2821 cggcgtcagc ggcgtccaat atttcgcgcg cgatttcgcg gcggtcggcg ccgaggcgtt
     2881 cgtgccgccc aacgagacca gccaattggg cctgttcacg ctccaggagt tcgatctcgg
     2941 caaggccggc atcgaagcgg cggggcgctg ggaacgcacc cgcgtcggct cgaacgaggt
     3001 cggcgtggcc cggaccttca acgcctattc gggcgcgttc ggcggttcct acgcggtgac
     3061 gcccaggatc aagatcggcg ccaatgtctc gcgatcggag cgggcgccgg cggccgagga
     3121 actattctcg aacggccccc atatcgcgac ccaggcgttc gagatcggcg accccgacct
     3181 gcaaaaggag aagagctggg gcgccgaagc ctatgtccgg gccgatacgc gcgacttcaa
     3241 cttgagcctc agcgccttcg ccaactggtt cgacgattat atcttcgagg ccgcgaccgg
     3301 ggaggagcag gacgagctgc cggtattccg ctatttccag cgcgacgccc gctattacgg
     3361 cttcgagggc gagatttcgg caaccttgtt ccgggcgggc ggcttccgct tcgtcgggga
     3421 cgtcgtcgcc gattacgtgc acgcctcgat caaggagggc ggcccggtgc cgcgcatccc
     3481 gccgctccgg gtgctggccg ggatcgaggc gcagtcggaa tccatcgacg ggcggatcga
     3541 ggccgaatgg tccgccgacc aggaccgggt cgcggcgttc gagacgccga ccgacgggca
     3601 tctgctcgtc ggcgcctcgg tggcgtggcg gccgtggggc aagcgcaacc ccacgacctt
     3661 cctagtgtcc gccaacaaca ttttcgatgt cgacgcccgc cgccacgcga gcttcaccaa
     3721 ggactatgtg ccgctcgccg gccgcgacat ccggctcagc gcccgcttca gcttttagca
     3781 gcgtcccgga tcagctccgc gacccggggc atggccgtat aatggaccat atggccggag
     3841 ccctcgatga gctcgagccg gctgccggcg accgcctcgt gcagccgggc cgattgggcg
     3901 agcggatcga ccagccggtc gccgcggccg gcggcgaggg tcagcggcag cccgagatcg
     3961 gcatagcgcc cggccagggc cgccgcggcc ggcaccatca aggccgcttc ggccgcggtg
     4021 gcgcggatct ggctcgggcg cagcgccatc tccatgggga aaagctcgaa tttgtccgga
     4081 acgggagcgg gcgcgaagat cttctggcga attttgggct gcagcgcggc gccgagcagc
     4141 ggcgagatcg tgtagcgcat gacgtcgccc agcaccggga ccgcaggctg ggaggcgaac
     4201 agcacgtcgg cgcgggctga ggggtaataa tagccgccga gcagcaccag gccattgacc
     4261 aggctggggt gatcgagcgc cagcgccaag gccaccattg tgccgaagct gtggccgacc
     4321 accagcgggc gcttgacgcc gatccgcggc agcgccgccg cgatcagctt ggcctgcgcc
     4381 gccggagtcc agatcgtggt gcgggggcgg tcactatggc cgaagccggg gcggtcgatg
     4441 gcgatcacgc ggtgcgagcc ggcgagttcg tcgagaaggc cggaggcttc ccaatcctcg
     4501 atcatggcgc cgttgccgtg cagcagcagc aagggctcgc cctcgccgcg ctcgacataa
     4561 tggaggcgga cgccgtcgac ggtgacgaat tgcccgagcg gcgggttgtc gcgctcggcc
     4621 cggcgcgcgc gacggtgatt gacgaccgcc gtcgcggcga gcgccgcgcc ggccgcgcct
     4681 gcgccccagg tccagagtcg gcggtcggat gcgccggcgt cgctcgcgcg ggaataggtg
     4741 gcactcttgt cgtgcttgct cataacggcc tttcgatgat ggatgtgttt ggtagccgaa
     4801 cgaggcggcg cgcctaaggt tgccccattc tcgctgccgc ctgcggctgg acaccgtccg
     4861 cattcggccg gaacggagca gattgatgcc gctcccggcc gccgcgccgc cctactcgct
     4921 ccgctttgga tcatgatcga attctgaccg attccttcgg cctttagcct ttcccccggg
     4981 gccggccgat ccggcgcggc ccggaatggg gaggcacaac atgatcatga ctcaggcgcg
     5041 cggccgcatg gccgcgcttg cgctcattgc gctgagtttc ggcgcggccg cgtcgccggt
     5101 ccaggcccgg caatccgatg ctcaaatcag gcagtcgatc atcaggcaat cgatagccaa
     5161 ttattccggg aactgcccct gtccctacaa caccatgcgc aacggccgcc gctgcggcgg
     5221 caacagcgct tacagccggc ccgggggaga atcgccgaaa tgcttcgccg acgacgtcag
     5281 ccagtcggaa gtgaacgcct accggcggcg ctgatgccct gcctcgggat caggcttgcg
     5341 gaagctgcat ctggtgttgc aatggcagtc acggtcccga gggaggcttg attgaagatc
     5401 gagggcgggt gtcactgcaa ggcggtgcgg ttcgaagccg aggtcgccga gcaggtcgag
     5461 gtgctcgact gcaattgctc gatctgcgca atgaccggct ttcgccacct catggtcccg
     5521 cacgaacgct tccggctgct ggccgggcag ggggcgctca cgagctatcg gttcggcacg
     5581 ggcgcggccg accatttgtt ctgcggcatt tgcggcgtga agagctttta ccagccgcgc
     5641 tcccacccgg aggcctggag cgtcaacctc aatgcgctcg acgactccgg ctcgctgcgg
     5701 atcagcgtca aggcgttcga cgggcgcaac tgggaagccg ctcgcgcggc gctcgattag
     5761 ctccaggcct cgcgccccgc ggctgcggtt ggacgggagc gatgggggcg gctatcggct
     5821 cggcggtgaa cccgccccat cgcccgctcc gcctgaccat cgacagcgcc gccctcgtct
     5881 cgaactggcg gtggctggcg cggatgagcg ccggggcggc ctgcggcgcg gccgtcaagg
     5941 cggacggcta cggcgtcggc gccgccgaag tggcgcggca tcttgccagc gccggctgcc
     6001 gggacttttt cgtcgccacc tgggccgagg ccgaggcggt gatgccggcg ctggccgggg
     6061 agtcgctgtc ggtgctccac ggcgtcggcg aggacgatgt cgcttatgcc gccgcgtcgc
     6121 cggcgcggcc ggtggtgaac accccattgc aggcgaagag gtggcgtgag cacgggcagg
     6181 ggcggccgtg cgacgtgatg atcgacaccg gcatgaaccg gctcggcctg tcggcggagc
     6241 aggtggcgga gggcgcgctg gacgggctcg ccgtcgacac cctgatgagc catctcgcct
     6301 gcgcggacga ggatgtcgcc gccaatgcgc ggcagcgcgg cgccttcctg aaggtggcgg
     6361 cgaaggttgc ggccaagcgg ctcagcctcg ccaacagcgc cggcatctgc ctcggccccg
     6421 attatgcgtt cgacctcacc cgtcccggcc tggcgcttta cggcggcatc ccgcgggcgg
     6481 aggcggaggg gcatatccgc caggtggtga gggtcgaggc cgaaatcgtg cagcgccgcc
     6541 gcatccgctc cggcgacagc gtcggctata acgccacctt caccgccgat ggcgattgcg
     6601 aactcgccat cctcaacctc ggctatgccg acggctattt gcgggacttt tccggcaccg
     6661 gccgcgcccg catcggcggc ggtttcgcgc ccgtcgtggg ccgcgtctcg atggacctga
     6721 ccgcgatccg ggtcgacgcg gcgcccgagc ttcgcgaagg cgattgggtc gagctcgact
     6781 tcgacttgcc cgccgcgtcc gcccagtccg gcctctcgca atatgagttg ctgaccacgc
     6841 tcggcgcccg ctatcagcgg ctctggtcat gaaagccggc gtcctcgctc ctgtgatagc
     6901 ggccttcgcg gccatcggcg cgacagtcat cgggctgttc gcgacggtcg ggcgcgtggc
     6961 cctgttcgcc tggacaaccg tgataagggc gctgatcccg ccttattacc cgctgcgcct
     7021 gctcgaacaa ttgctccaca tcggctggta ttcgctgccg gtcgtgggcc tcaccgcggt
     7081 gttcaccggc gcggcgctcg cccagcaaat ctatgtcggc ggctcgcgct tcaacgccca
     7141 gtcgaccgtg ccggcgatcg tggtcatcgg catcgtccgc gagctcgggc cggtgctggt
     7201 cggcctgatg atcgccggcc gcgtctcctc ggcgatggcg gcggagatcg gcaccatgcg
     7261 cgtcaccgag cagctcgacg cgatggcgac gctcagcacc gacccttacc gtttcctgat
     7321 cgccccgcgc ctgttcgcct cggtgatcgc tttgcctttg ctggtgctgg tcgcgaacgc
     7381 gatcggggtg ctcggcggct tcatcctcgg cgtctacaag ctcggcttca acgccaccgc
     7441 ctatctcgaa acctcgcgcc gctacatgca ggaagacgac ctcgtcatgt cgatggtgaa
     7501 ggcggccgtg ttcggcttct tcatcgcttt gatgggctgc tatcacggct tccgctcggc
     7561 cggcggggcg gcgggcgtcg gcaaggcgac caccaacgcc gtcgtctcgg ccttcatcct
     7621 gatcctgctc gccaacctcc tcatcaccgt cgcggcgttc ggatgagcgc gccccccaag
     7681 atcgcgatcc ggggcctcgc caagaggttc gacgacaatg tcgtcttcga cggcgtcgac
     7741 ctcgacgtgc agcccgccga atcgatcggg gtgatcggcc agtccggcat gggcaagtcg
     7801 gtgctgatca aatgcatcct cggcctgatc gagcccgacg ccgggtcgat cctgatcgac
     7861 ggcgaggaga tggtcggcgc cggcgagcgc aaatgggaca agtgccgggc caagttcggc
     7921 atgctgttcc agggcagcgc gttgttcgat tcgcttccgg tctggcgcaa cgtgaccttc
     7981 ggcctcaccc aggggcggct gcagaacccg gcgcggatgc gcgccatagc cgaggagaat
     8041 ctggagcggg tcgggctcgg gcgcgacgtc ctctatctgc ggccctcgga gctttcgggc
     8101 ggcatgcaga aacgggtcgc gctggcccgg gcgatcgcgc cgcgtcccga gatcatcttc
     8161 ttcgacgagc ccaccaccgg cctcgatccg atccgcgccg acgtcatcaa cgacctcatc
     8221 gtcagcctgg tcgaggatct cggcgtcacc gcgctcacca tcacccacga catggcgagc
     8281 gcgcgcaagg ttgcccaccg cctggcgatg ctccatggcg gccgcatcat ctggcagggc
     8341 tctcgcgacc ggctttacga cagcggcaac ccgttcgtgg accagttcgt tcagggccgt
     8401 gccgagggtc cgatccacta ggcgcggagc ggccgcggca ggaacccgtt cgggcccccg
     8461 ggagggcgag tgatagaagc gcatctgtcg aagctgagag cgcgggatga gctttccgag
     8521 gaggaggagc gggcgattcg cggcgcggtt tccgaggtca gggaattccc cgccgacaag
     8581 acgatcatcc gggcggagca ggaattgtcg gtcagcaccc tgctgctcga cgggctgatg
     8641 tgccgctaca aggatctgcg cgacgggcag cggcagatca ccgagctcca cgtcgccggc
     8701 gatttcgcag acctgcacag catgaccctg aagcggctcg accataatgt catgacgctg
     8761 acgccctgcc gcgcggcgat cgtcccgcac gaccggctga aggcgatcac cgagcagcat
     8821 ccgcacctca cccgcgtcta ttggttctcg accaacatgg atgccgccat ccaccgcgaa
     8881 tgggcggtct cgcttggccg gcgcgcggcc ctgtcgcggg tcgcgcacct gttctgcgag
     8941 ctttacgtcc gcctcggcat cgtcggcctc gccgacgaca caggctatgc gctcggtctc
     9001 acccagaccg accttgccga atgcctcggc ctcacctcgg tccatatcaa ccgcaccttg
     9061 aaggagcttc gcgagcgcgg cctggtcgag tttcgcggcg gccgggtgac gatccacgac
     9121 cgggccggac tggagcgggt cgccgagttc aaccccagct atctctacct ggagcggcgc
     9181 cgccgctgac ccggcgtcag gggcgggatc ctcatcccgc gagagccctg ttcaccgcgg
     9241 ccgctctatc ctcctggcgg aagggcttcc tcagcaccgg cgcgtcgccc gcggccgctt
     9301 cgatggcggt gtcggcatag ccggtgacga acaggatctt ctgctccggc cgcgcctgcc
     9361 ggacgacccg ggcgacctcg gctccgttca tccccggcat cgcgaagtcc agcaccagca
     9421 gatcgaagct ctcctcctga aggaggtcca ggccttcctg gccgctggag gcggcgctgg
     9481 tccggtggcc gagctcggcg agggtgtcga ccaggacctg gcgcacgtcg gcgtcgtcgt
     9541 cgaccacgag gatcctcgcc gcggccccgt cgcccgccgg gccggaggct tgatcgggct
     9601 gcgccggctc gatcgcggcc tcgccggtgc ggggcaggag gatggagagg gtggtgccgg
     9661 cgccgggctc gctctggatc cgcgcgatgc cgcccgattg ccgcgccacc ccatagacca
     9721 tgctgaggcc gagcccggtg ccctggccga cggccttggt ggtgaagaag ggatcgaacg
     9781 cccgcgccgc cgtctccgcg tccatgccgg cgccggtgtc ggagacggtg aggtggagat
     9841 attcgccgtc tggaagatcg ggatcgtcgc ccagctggcg cggccgcgcg gcgatggtga
     9901 gggtaccgcc gcccggcatc gcgtcacggg cgttgatcgc catattgagg atggcgagct
     9961 cgagctggtt ggcgtccgcc atgaccggga cttcggcatc gtcgagcgcc agctccagcg
    10021 ccaccccggg ccccaaagta tgcgagagaa gctcgcgcat gccgcggatc agcggcgcga
    10081 caaggacggg gcgcagctcc aggcgctggg tgcgggagaa agcgagcaat tgcccggtaa
    10141 gcttggcgcc gcgcgcggcc gcctgcagcg cgctgtcgac cagccggccg gtgcgcggct
    10201 cggcggtcga gcgccggatc atgtcgagat tgcccagcac caccgtcagc agattgttga
    10261 aatcatgggc aatgcctcca gtgagctgtc cgaccgcctc catcttctgg gcctgcacca
    10321 aagcggcctc ggcgcgctcg cgatcctgga tctcgcgctt cagccggtcg agggcgtcgt
    10381 cgcgctcgcg gacgcgctcg ctcaactgca cgttggcctc cttgagctgg agcggcgagg
    10441 ggatggcgag cgccttgggg atgagcggcc agagcacgat cgcggtgccg accgaggcga
    10501 tggcggtgac caccttgatc agcgcctccc agccataatc ggggttccac aaggtccaga
    10561 tcgccatcaa atgggtggtg ccgcaggcga ggatgaacag ggcgaacagc cagaatatcc
    10621 agctgaactc caggtcgctg cggccgcgca cgaagcgcac gatcgcgacg gggatcgaga
    10681 aataggcggc ggcgatcagc gcatcggcga ggacgtgggt ccagaccaga gccgggtccc
    10741 acaggaggca gtaaccgtgc ggcgcgagat cgcgggcgcg gctgaagata tccagaaatt
    10801 ccagcatcaa agccctcctg aacgggagct atgaccggcg gcgggcaggc gtgcaatgcg
    10861 tgcttcatgc ccctgacgaa gggggacgag cctcagcgct tgctcaggaa gctgcggggg
    10921 agaccggcac tccgaacagg tcgtgctcgt cggcgtcctc gaccagcacc ctgaggatgt
    10981 cgccgggcct ggcgccatgc gcatcgcgca ggtggacctc gccgtcgatc tcgggcgcgt
    11041 cggccttgga gcggccggtg gcgccgccct cttcgtccgc caggtcgacg atgacgtcga
    11101 tattgcggcc gaccttggcc tggagcttgg cggcggagat ggcggcggtg cgctccatca
    11161 gccgggcgta gcgctcttcc ttaacttcct ccggcaccgc gccggggagg gcgttggcgg
    11221 cggcgccttc gaccggctcg aaccggaagg cgccgacgcg gtcgagccgc gcttcgtcga
    11281 gccagtcgag cagatattgg aaatcggcct cggtctcgcc ggggaagccg accacgaagg
    11341 aggagcggat cgcgatgtcg gggcagatgg cgcgccaggc gtggatccgc tccagcacct
    11401 tggcttcgtt ggccgggcgc ttcatcgcct tcagcaccga cggggctgca tgctggaacg
    11461 gaatgtcgag ataggggagg accagctcct cggccatcag cgggatcacc cggtcgacat
    11521 gcggataggg gtagacgtag tggagccgca cccaggcacc gagcttgccc agctcacggg
    11581 cgaggtcggt catgtgggcg cggaccggcg cgcctttcca gttccattcg gcgtggcgca
    11641 agtcgacgcc gtaagccgag gtgtcctggc tgatcaccag caactcacgg gtgccggcgg
    11701 cgattagctt ctccgcctcg cgcaggatcg cgtcggggcg gcggctggcg aggtcgccgc
    11761 ggatcgacgg gatgatgcaa aagctgcagc gatggttgca gccctcggaa atcttcagat
    11821 agctgtaatg gcgcggggtc agcttgaggc cgccgtcggg caccaggttc aggaaggcgg
    11881 acggcggcat cggcgcggct tcgtggaccg cgccgacgac ctgctcatat tggtgcggcc
    11941 cggtgacggc gagcacttcg gggaaccggg cgcggatcac ctcggcctcg cggcccatgc
    12001 agccggtgac gatgacgcgg ccattctcgg cgatcgcctc gccgatcgcg gccaggctct
    12061 cctccttggc gctgtcgagg aagccgcagg tattgaccag gacaacatcg gcggcgtcgt
    12121 aatcgggcga gagttggtag ccgtccgaac gcagcttggt gaggatccgc tcggaatcga
    12181 ccagcgcctt ggggcagccg agcgaaacca tgccgacctt gggcggggag gggagcgtca
    12241 ttaccatggg aggcgcccac atagtgtgat ccgccgcttt ttcctaggcc tgcctggcgg
    12301 ggacggggcg gcggcgatcc cctcgaaaat ttgcggccgc gcctttaaca gcagcgcagc
    12361 ccgagactta gcccaatcat caggcttccg tcagcacttc atcagggcga ggctgttccc
    12421 tccctcgtcc ggccgccgca cgctctgatc gaaacgggcg cgggggggcg gcatggcttt
    12481 aggttcgaac tcgatcaatc aggtggagct gagcggcaat cccgacatcg acgcgctgct
    12541 gacaggccat cgctgggcga acgtggtcgt gacctattcg tttccgacca gccagggcga
    12601 atattcctac catgcgatca ccggcttcga atcgttcaac gtggaacagc gcgcggcgac
    12661 ggaccagatc gtcgccaatt tgaacgccgt ctgcggcctc aacatcgtcc ccggcgggcc
    12721 gggctccaac atccggctcg ccgaggccac caccatcgac tatacgcagg tcgacgccga
    12781 acccgcccat gggccgggcg gcgagaacga ttccgccgag gcgtcgcctc cccaacccga
    12841 tgcgtcgtac cgcgtcggcg acacctggta caaccacagc gactataaca atccggtcgc
    12901 cgggactttc gcctacgcgg gcgggctgat gcacgaaatc ggccatgcgg tcggcctcgc
    12961 ccacggccat gagacggacg agatcgagca tagccacggc gtgcatccca agccggcttt
    13021 gccccgggat catgacacgc aagaatattc gatcatgacc taccgccaat atgtcggcga
    13081 ggccgacccc aatgacggta acaatgggcg cgaccatgcc cagtcgctga tgatcaacga
    13141 catcgccgcg ctgcaatatc tgtacggcgc ctattacggc caccggagca acaattcagt
    13201 ctattcgtgg agtccgacca ccggcgaaat gtcgatcgac ggcgtcggcc agggggtgcc
    13261 gtggatcgtc gagctcgcgg atgggacgct caagagcacc gtctttcgca ccatctggga
    13321 cggcggcggc atcgacacct acgacttctc caattaccgg accgacctcc tcgtcgacct
    13381 caaccccggc ggctggagca cgatcagcga agatcagctc gccctcctgg gcaatgacgg
    13441 aatgagggac cagttcgcgc gcggcaacag cgccaacgcg ctgctccatc agggcaacct
    13501 ccagtcgatc atcgagaacg ccaccggcgg ctccggcaac gaccgcttgg tcggcaacgt
    13561 cgtcggcaat ctgctgctcg gcaatccggg ccgggacacg ctgttcggca atgaaggcaa
    13621 tgaccgcctg aacggcggca cgggtgcgga cgcgctgatc ggcggcgacg gcatcgacca
    13681 ggcgagctat gtcggcgcct tcgtcgacgg ggtcacggca gacctgatat tcgccacagc
    13741 caatagcggc gacgccgagg gcgacagcta cgattcgatc gagaacctgc aaggcaccag
    13801 cttcgacgac gacctccgcg gccacgatgg cgagaacgtc ctcgagggcg gcttcggcgg
    13861 cgacgaccag ctttacggcc gcgccggcaa cgacctgctg atcggcgccg acggcgacga
    13921 cctgctcgaa ggcggcgcag gcgccgacgc cctggacggg ggcggcggca acgacaccgc
    13981 aagctacctt tttgcggact cgcgcatcct ggccgacctg ctgttcgact ttcgcttcac
    14041 caacgaaggg gatgcgctcg gcgataccta tgtgggcgtc gagaacctga tcggatcgcg
    14101 gttcgacgac gatctgcgcg gcgacgatgc ggccaacatc ctgtcgggcc gcggcggcac
    14161 cgacgggttc aacggccgcg gcggctacga cacgctgatc ggcggccgcg gcagcgacct
    14221 gttcttcctc caggaccttg cctatgccgg gcagttcgcg ggcttccaat atgaccgcgt
    14281 cgtcgaaacc cccgacggcg gcgtcgatac cgtgttcgtc accgcgttcg acaatcccga
    14341 cgatttctcc acctccggct acacgctcgg cgacaatgtc gaatatggcc aaataagggg
    14401 ctcggtcgcc ttcaacctca ccggcaacgc gctcgacaac gtcctcaccg gccacggcgc
    14461 cgccaaccgg cttaccggcc acgccggcag cgattatatc agcggcaatg gcggcatcga
    14521 catcctgatc ggcggggccg gcgacgacac ctatgtgctg
//
LOCUS       DASVRK010000204         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_537370, whole genome shotgun sequence.
ACCESSION   DASVRK010000204 DASVRK010000000
VERSION     DASVRK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537370"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VF589_08005"
     CDS             complement(<1..622)
                     /locus_tag="VF589_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX8257563.1"
                     /translation="MSSPQPDEAVLAANLDQALGFTIPGRHARGRLVRLGPVLQEILA
                     AHDYPAPIARILSEALTLTGLLGAMLKDVGGQLTLQAQTEAGIIDLLVCDYRAGELRG
                     YIRFDRERLAEAPVLPSLFALFGKGYLAITFDQEVSGERYQGIVPLEGNSLADAAQSF
                     FGQSEQIPSLVRLAVNDTGHIAGGLLVQHLPEGEEGRERLHTRLDHP"
     gene            complement(671..1861)
                     /locus_tag="VF589_08010"
     CDS             complement(671..1861)
                     /locus_tag="VF589_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8257564.1"
                     /translation="MAITPLMPVYPRSPVRPVRGEGVYLYGEAGEQYLDFASGIAVNL
                     LGHGHPHLTEAIQRQAATLIHVSNLYGSPQGEAYARRLCERTFADTVFFTNSGAEAVE
                     GAIKTARRYHHHHGRPEKNVLITFKNAFHGRTLGTISATDQEKLRDGFAPLLPGFTVV
                     EFDNLEAAEAAVTEHTAGFLIEPIQGEGGIRPASHDFIRGLRRLADENDLMLVFDEVQ
                     CGMARTGKLFAYEHYGVEPDILASAKGIGGGFPMGAFLATEKAAAGMAIGTHGSTYGG
                     NPLAMAAGQAVLDVVDTPEFLEHVAQIGERLRGALEQMIPNHDHLFESVRGIGLMLGV
                     RMKTDSRAFVGYLREHGLLTVAAGDNVMRVLPPLVIEEAHVTEFVERLSEAARRYDMP
                     QVAA"
     gene            complement(2264..>2844)
                     /locus_tag="VF589_08015"
     CDS             complement(2264..>2844)
                     /locus_tag="VF589_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257565.1"
                     /translation="ALISAAIMASPAAAQYYSRPSYGQHSQLDWRLDRVQYAIEQAYR
                     SGRISPSEASRLRAEADWIEQVHYRHRRHGLSYTEVRNLQGRIETLRRHLRIESRDWN
                     GYASNGGYGQGGYGGYGQGGYGGYGGYPSGGYGQVGYGQGGYGQGGYGQGGYGQGGYG
                     QGGYYPNDDRYDDGDRYDDDDRGGYPDPYDDD"
BASE COUNT          444 a          945 c          995 g          460 t
ORIGIN      
        1 cgggatggtc gagccgcgtg tggagccgct cgcgcccctc ctcgccctcg ggcagatgct
       61 ggaccagcag ccctccggcg atatggccgg tatcgttgac ggcgagccgg accaggctcg
      121 gtatctgctc ggactggccg aagaagcttt gcgccgcgtc ggcgaggctg ttgccttcga
      181 gcggcacgat gccctgatag cgctcgcccg acacctcctg gtcgaaggtg atggcgagat
      241 agcccttgcc gaacaaggcg aacagcgacg gcagcaccgg cgcttcggca agccgctcgc
      301 gatcgaagcg gatatagccg cgcagctcgc cggcgcgata gtcgcagacg agcaggtcga
      361 tgatgcccgc ttcggtctgc gcctgcagcg tgagctgccc gccgacgtcc ttgagcatgg
      421 cgccgagcag cccggtcagc gtcagcgcct cggacagtat ccgcgcgatc ggcgccggat
      481 aatcgtgcgc cgccaatatc tcctgcagca ccgggccgag ccgcacgagg cggccgcgcg
      541 cgtgtcggcc ggggatggtg aagccgagcg cctggtcgag attggcagcc agcaccgctt
      601 cgtcgggttg tggagagctc atcggcgcca tgtggccagc ccctgcccgc ggttcaatat
      661 ccccgccggc tcaggccgcc acctgcggca tgtcgtagcg ccgcgcggct tcggacaggc
      721 gctcgacgaa ttccgtgaca tgcgcctcct cgatcaccag cggcggcagc acgcgcatca
      781 cattgtcgcc ggccgcgacg gtcagcaggc cgtgctcgcg caggtagccg acgaaggcgc
      841 ggctgtcggt cttcatgcgc acgcccagca tcagcccgat gccgcggacg ctttcgaaca
      901 gatggtcgtg attggggatc atctgctcga gcgcgccgcg cagccgctcg ccgatctggg
      961 cgacatgctc gaggaattca ggcgtatcca caacgtccag caccgcctgg ccggccgcca
     1021 tggcgagcgg gttgccgcca taggtcgatc cgtgggtgcc aatcgccatg ccggcggccg
     1081 ctttctcggt ggcgagaaag gcgcccatcg ggaagccgcc gccaatcccc ttggccgagg
     1141 ccaggatgtc gggctcgacg ccgtaatgct cgtaggcgaa cagcttgccg gtgcgggcca
     1201 tgccgcactg gacctcgtcg aacaccagca tgaggtcgtt ctcgtcggcg agccggcgca
     1261 ggccccggat gaagtcgtgg ctggccgggc ggatgccgcc ctcgccctgg atcggctcga
     1321 tcagaaagcc ggccgtatgc tcggtaaccg ccgcttcggc cgcttcgaga ttgtcgaact
     1381 cgaccacggt gaagcccggc agcaaaggcg cgaagccgtc gcgcagcttt tcctggtcgg
     1441 tggcgctgat cgtgccgagc gtgcggccgt gaaaggcgtt cttgaaggtg atgaggacgt
     1501 tcttctcggg ccggccgtga tgatggtggt agcggcgcgc ggtcttgatc gcgccctcga
     1561 ccgcctcggc ccccgaattg gtgaagaaga cggtgtcggc gaaggtccgc tcgcacagcc
     1621 gccgggcata ggcctcgcct tgcgggctgc catagaggtt ggagacgtgg atcagcgtcg
     1681 ccgcctggcg ctggatcgcc tcggttagat gcgggtggcc gtggccgagc agattgaccg
     1741 cgatgccgct cgcgaaatcg agatattgct cgcccgcctc gccatagaga tagacccctt
     1801 cgccccgcac cggccgcacc ggcgaacggg ggtaaacggg catgagcggg gtgatggcca
     1861 tgacttcctc gatcgggcaa ggtggagcga aaaaggcgac ccgcggcggc cgccctcagg
     1921 tcaggcgcta tactgatcgg ccgggcggcg cgtcaatcgg agcctcgttg aagggtcgtg
     1981 cgcgcgaaac ctggcatctc gcttcctggg agcctgcgcc acgtgcccct gcgaaggcag
     2041 gggtccagac ccgggttgcc gcttcagcgg cagcccggtc atcgcaccgg cggtggaagc
     2101 agggcgccgt agcccgcacg gttccggact cgcgcgatgt ctccgagggc tcgatggacg
     2161 ggctttcgcc cgggctgggc ccctgcctcc gcaggggcac cagaaacgaa agacccggcg
     2221 gggaagaaga agggagattc cagctttcgg tggaatgacg agctcagtcg tcgtcgtaag
     2281 ggtcagggta gccgccgcgg tcgtcatcgt cgtagcggtc gccgtcgtcg tagcggtcgt
     2341 cgttcggata gtagccgccc tgaccgtagc cgccttggcc atagccgccc tgcccataac
     2401 cgccctggcc gtagccgcct tggccgtacc cgacttggcc gtaaccgccg gaggggtagc
     2461 cgccgtagcc gccgtaaccg ccctgcccgt agccgccata accgccctgc ccatagccgc
     2521 cgttactggc atagccgttc cagtcgcggc tctcgatgcg caggtgccgg cgcagagtct
     2581 cgatccgccc ctgcaggttg cggacttcgg tataggacag gccatggcgg cggtggcgat
     2641 aatggacctg ctcgatccag tcggcctcgg cgcgcaggcg cgacgcctcg gacggcgaga
     2701 tccggcccga gcggtaggcc tgctcgatgg catattggac gcggtcgagc cgccagtcga
     2761 gctgactatg ctggccgtag ctcgggcggc tataatattg ggcggccgcc gggcttgcca
     2821 taatggcggc ggagatcaag gctg
//
LOCUS       DASVRK010000205        14507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_101110, whole genome shotgun sequence.
ACCESSION   DASVRK010000205 DASVRK010000000
VERSION     DASVRK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 14507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14507
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101110"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF589_08020"
     CDS             complement(<1..481)
                     /locus_tag="VF589_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX8257566.1"
                     /translation="MSFPWAEPYEPKSGFMRWIDQRLPLPRLVFNAVGAGYPVPRNLN
                     HFWNFGVLAGVALTIQIVTGIILAMHYAPAGAMAFDSVEHIMRNVNQGWLMRYAHANG
                     ASFFFIVTYIHIFRGLYYGSYKAPREMVWLLGLVIFLLMMATAFMGYVLPWGQMSFWG
                     "
     gene            complement(493..1080)
                     /gene="petA"
                     /locus_tag="VF589_08025"
     CDS             complement(493..1080)
                     /gene="petA"
                     /locus_tag="VF589_08025"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX8257567.1"
                     /translation="MATAQQSDGTPPAGAGAEAAAEPGVRRRDFINIAAVSFAGVGAV
                     ATLYPLINQMNPSADVLALASIEVDVSQIQPGQAVKTIFRKQPLFIRHLTPPEISQAN
                     AVPVGELRDPQSLAERTAEGKPQWLITMGVCTHLGCVPLGAAEGENKGDYGGYFCPCH
                     GSHYDTAARIRRGPAPTNLEVPEYAFKSDTIVQIG"
     gene            1389..1556
                     /locus_tag="VF589_08030"
     CDS             1389..1556
                     /locus_tag="VF589_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257568.1"
                     /translation="MEDRKQRPEAEDETRPQRGGEAATTRFGMEDGDNTDVPATGGPA
                     ADEAGEQDGGG"
     gene            complement(2032..2163)
                     /locus_tag="VF589_08035"
     CDS             complement(2032..2163)
                     /locus_tag="VF589_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin EcnA/B family protein"
                     /protein_id="HEX8257569.1"
                     /translation="MRKILAIVAISSSLILAACGTVRGAADDVESVGDCVDGREGNC"
     gene            2359..2721
                     /locus_tag="VF589_08040"
     CDS             2359..2721
                     /locus_tag="VF589_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HEX8257570.1"
                     /translation="MSAGQNAWQAGMPASVRSRIEADIPGLRFAESIEPPLDFAHLFT
                     KSRAHLEEQLVRLRSALAPAGMIWVSWPKKASNVATDITEDTIREVALPLGLVDVKVC
                     AVDETWSGLKLVIRRSLR"
     gene            complement(2740..3159)
                     /locus_tag="VF589_08045"
     CDS             complement(2740..3159)
                     /locus_tag="VF589_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8257571.1"
                     /translation="MAEIKTKPTQANVDEFMAAVAKPGRRADAEVLRDMMERVTGEPA
                     TIWGPTIVGFGTCHYRHDSGREGDMCRVGFSPRSANLVLYVGGFPEYEALLGKLGKHK
                     RSQACLYVNKLADVDMPVLEEIVRRSFNASDDVDQGV"
     gene            3296..3724
                     /locus_tag="VF589_08050"
     CDS             3296..3724
                     /locus_tag="VF589_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257572.1"
                     /translation="MDTASPASPPRWFRAVALLAVIWNAVGVATYLSSVGLFGDPMAG
                     LSETERAAASSMPGWITGLFATGTFAGLAGSVGLLLRKAWALPVLIVSLVALLVLEGW
                     VLFLSDAVERFGVAVPVMVSAGAILLAWLASHARQRGWLS"
     gene            3754..4203
                     /locus_tag="VF589_08055"
     CDS             3754..4203
                     /locus_tag="VF589_08055"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX8257573.1"
                     /translation="MRLALFEPDQAGNVGTILRLGACLGVPVDVIEPCGFPWSDKALK
                     RAGMDYAAMANVTRHADWAAFGARLAGRLALFTTAGAVRLPEARFEPGDTLLFGSESR
                     GAPEAVHERADLRVRIPQAEGTRSLNLAVAAGIGVAEALRQTHLWAG"
     gene            complement(4434..5999)
                     /locus_tag="VF589_08060"
     CDS             complement(4434..5999)
                     /locus_tag="VF589_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257574.1"
                     /translation="MRLKRSNARLAAAATDGNDRLVGTNQAEILAGEAGDDWIYGRGG
                     DDTLDGGDGDDRLDGGLGFDRLNGGLGNDTYVLATAGDRVVEALDAGRDTVRAAISYT
                     LTANVETLLLVGGALDGTGNGLANLIVGNSAANRLDGRGGADRMSGGDGDDRYLVDDL
                     GDIVREAADGGSDTVESRVSHRLAANVENLTLLGARAIDGTGNSLFNHIVGNSADNVL
                     DGGVPAEDAHGGNDILEGGAGNDTYVLHMIPNTSGSSDTIIIEAAGQGIDTILTDVRL
                     QGDLPLNVENLTMFGNEDVDADGNGLSNRIVGNGRTNALDGKGGEDTLIGGAGDDIYM
                     NVNAAGADTVVERAGGGHDAVYAVGTYVLAANVEDCFAWSRDVKFTGNGLDNYMGGSA
                     GEDVLVGGAGEDWLSGRAGSDVLTGGTGTDKFSFSYVPDGQVDRITDFHAGSEKIVLW
                     NDFIPAGPAGSLAAGAFRTGTAAQDADDRIIYNKANGNIWFDADGNGSGAAVLLARVD
                     AGLALSHTNFEVV"
     gene            6296..7483
                     /locus_tag="VF589_08065"
     CDS             6296..7483
                     /locus_tag="VF589_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257575.1"
                     /translation="MRTLTNILGAAALLCAMAPAAAPAAQPLPQNPASAEASIDGLWR
                     GEVGGRTVLACFNATIGSFYAPADRKLIMLWAEQPLRWAEKDERDPANPRWNLRLQGN
                     DRLAGERAAGRDAQALTLARLPAPAAEDADSDATGPCRSLAYHRERLEPLAVRERPAR
                     LGTAGYVEIVAAPASYPDAVRYQSFRVPGDAPGTAAVNALLARMIDLRGPGESEWSLC
                     LAAAGNSGGDFHAGLAPVRIGRRFVAAEEEREASCGGAHPSSGSYPRLFDLVEGREVA
                     MRDWLNPRALDGELLDERSEPNPDAEPNVLPLSAALRRVLVSKAGKPEPECAGTVDEE
                     TYWTVGVGRDALLFYPHLARAVRACGEEIALPFTAMAPFLSAEGKTALARLRADPAVF
                     AQP"
     gene            7529..8392
                     /gene="hemF"
                     /locus_tag="VF589_08070"
     CDS             7529..8392
                     /gene="hemF"
                     /locus_tag="VF589_08070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX8257576.1"
                     /translation="MIEMDEQQQAARAWFESLRERICAEFEAIEREAGSAAAFEYLPW
                     DRLDPDGSPGGGGVRGLMKGQVFEKVGVNVSTVGGRFSPEFARTIHGAEEDPSFFATG
                     ISLVAHMANPHVPAVHMNTRFLVTTKSWFGGGADLNPPLPYEEDTADFHARLRAACAA
                     HDPTYYPRFSKWAEDYFFIPHRGVSRGVGGIFYDHLECAERGQGAGFDEHFAFTRDVG
                     EAFLDIFPRIVRRRMNTPFSAEDKARQLEWRGRYAEFNLIYDRGTLFGLRTGGNVDAI
                     LMSLPPLATWG"
     gene            complement(8727..9344)
                     /locus_tag="VF589_08075"
     CDS             complement(8727..9344)
                     /locus_tag="VF589_08075"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX8257577.1"
                     /translation="MPTDISSDWQQLALYAGGAALLLILLQRVPVIGRIVRFGLSFGL
                     LALCLFLLLQHAPYEPMLAPLADRLGLDRQEVVGGAVRVPMAPDGHFWANATLNDTKH
                     RMMIDSGATITAISEQTARASGIAHDATLVPVVLKTANGLARARTGSVRELRVGDIVA
                     SDLKIVITPALGDLDVLGMNFLSKLASWRVEGRTLILVPERPEAA"
     gene            complement(9427..10377)
                     /locus_tag="VF589_08080"
     CDS             complement(9427..10377)
                     /locus_tag="VF589_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8257578.1"
                     /translation="MRLVALLAWLLAFAAQPALAEVSPTLRSLEQRLAALAIENPGEY
                     GIAALDLATGATISFNGDRPFPMASTMKVAVAAAYLAEVDAGRRSLDDTVAGASAGRL
                     MDAMITRSDNRATDLLMATLGGPASIDRWLRAHNLSGIRVDRTIAQLLGDRRDLSDLR
                     DSSTPAAMLGLLRVIDAGVALKADSRVLLLDMMRRCKTGSNRIRGILPAGARVENKTG
                     TLNNYTGDVGFLTTPTGQRIAVAFFARGGSNRPAVIATAARAIYDAFGAEAQTPKRPA
                     WMPATQPLRAAMPQRLAPAPACPAPRLGQPAGTRPAACTQ"
     gene            10719..11072
                     /locus_tag="VF589_08085"
     CDS             10719..11072
                     /locus_tag="VF589_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8257579.1"
                     /translation="MDSPSAVSALGALAQEHRLALFRLLVRAGGEGMAAGAIAEALAV
                     PNSSLSFHLAQLHKAGLVRQERRHRSIIYRADYAVMNDLVGYLMENCCAGADCGAAPE
                     CGAGTGPAQLERTDI"
     gene            11069..11575
                     /locus_tag="VF589_08090"
     CDS             11069..11575
                     /locus_tag="VF589_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX8257580.1"
                     /translation="MKRLHVHVGVENLDRSVSFYSTLFGAGPSVLKGDYAKWMLDDPR
                     VNFAISAGNHAAKGVEHLGIQAESEDELAEVYGRLKAADRPMLEEGATTCCYARSEKS
                     WIADPDGVVWEAFLTNGEATVYGDSPALAALAEKDAETSGCCDAATPQGVCCEPKPQL
                     AADAPCCG"
     gene            11575..12666
                     /gene="arsB"
                     /locus_tag="VF589_08095"
     CDS             11575..12666
                     /gene="arsB"
                     /locus_tag="VF589_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX8257581.1"
                     /translation="MAAQPDAATLTDAAPPRPGMTRFERYLSIWVALCIAAGIALGAA
                     APGLFALIASAELARVNLPVALLVWLMIVPMLLKIDFAALGQVRQHMRGVGVTLFINW
                     AVKPFSMALLGAIFVGWLFRPLLPADQIDSYIAGLILLAAAPCTAMVFVWSNLCEGEP
                     HYTLSQVALNDVIMLFAFAPLVGLLLGVASIAVPWDTLLLSVLLYILVPVVVAQLIRR
                     RRLAVGGQPALDALLRRLGPVSLAALLTTLVLLFGFQGEAILAQPLVIALLAVPILIQ
                     VYFNAGLAYWLSRRFGVAWCVAAPAALIGASNFFELAVAAAISLFGLKSGAALATVVG
                     VLVEVPVMLSVVAIVKRTRGWYERADAVS"
     gene            12774..13370
                     /locus_tag="VF589_08100"
     CDS             12774..13370
                     /locus_tag="VF589_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8257582.1"
                     /translation="MPLTEVPPGHVGAVVTYLEMTVRPLPRPIPASPLRLAAWRPLVL
                     GKYRALFRRVGAPWLWFSRLAMDDAALAERTGEVWAVEDADGNEVGIVELDFRAPGEC
                     LIAFLGLVPELAGKGHGSWLFGEVLALAWRPGVERVHVHTCTLDHPAALKAYLKAGFT
                     AYQRAFESFADPRLLGLLPRGAAPQIPLVGPDASASRR"
     gene            complement(13325..14074)
                     /locus_tag="VF589_08105"
     CDS             complement(13325..14074)
                     /locus_tag="VF589_08105"
                     /inference="COORDINATES: protein motif:HMM:NF018496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HEX8257583.1"
                     /translation="MKFSMSAVWDDTRQMLAANASLVAAVAGVFLFLPTLAFGYFVPE
                     PTGGRDGPTLEMLMQHMRANWPLLLLVNLIGLIGNLALFILTLDRDDPTVGGAIKAAA
                     LLLPVYFLTSFLSGLVIFVGLVLFIVPGLYLMARLAVAGPAIVAERLRNPVDALRRSV
                     AVTKGNGWAILALLLVLILAYFVLSLAGVYVLGSIFLLIGRAAGAEGLGEMLLLIFST
                     ALGAAFYTVLMVLLASLYRRLAEASGPTSGI"
BASE COUNT         2245 a         4945 c         4966 g         2351 t
ORIGIN      
        1 cgccccagaa gctcatctgc ccccagggga ggacatagcc catgaacgcg gtcgccatca
       61 tcagcaggaa gatgacgagg ccgagcagcc acaccatctc gcgcggcgcc ttgtaggagc
      121 cgtaataaag gccgcggaaa atgtggatgt aggtgacgat gaagaagaag gacgcgccgt
      181 tggcatgggc gtagcgcatc agccagccct ggttgacgtt gcgcatgatg tgctcgaccg
      241 aatcgaacgc catcgcgccg gccggcgcat aatgcatggc gaggatgatg ccggtgacga
      301 tctggatggt gagcgccacc ccggcgagca cgccgaaatt ccagaaatgg ttcaagttgc
      361 gcggcaccgg atagccggcg ccgaccgcgt tgaacaccag ccgcggcagc ggcagccgtt
      421 ggtcgatcca gcgcatgaag ccggacttag gctcgtaagg ttctgcccag gggaagctca
      481 tctgttctta cctcagccga tctggacgat ggtgtcggac ttgaatgcat attccggcac
      541 ttcgagattg gttggcgccg ggccgcggcg gatgcgcgcg gcggtatcgt aatgcgagcc
      601 gtggcagggg cagaaatagc cgccataatc gcccttattc tcgccctccg cggcgcccag
      661 cggcacgcag cccaaatggg tgcagacgcc catggtgatc agccattgcg gcttgccctc
      721 ggcggtgcgc tcggcgagcg actgcggatc gcgcagctcg ccgaccggga ccgcattggc
      781 ctggctgatc tcgggcgggg tcaaatggcg gatgaacaag ggctgcttgc ggaagatggt
      841 cttgaccgcc tggccaggct ggatctggct gacatcgacc tcgatcgagg cgagcgcgag
      901 cacgtcggcc gacgggttca tctggttgat gagcggatag agcgtggcga ccgcgccgac
      961 gccggcgaag ctcaccgcgg cgatgttgat gaagtcgcgg cggcggacgc ccggttcggc
     1021 cgccgcctcc gcgcccgcgc ccgccggggg cgtgccatca ctctgttgcg ccgtcgccat
     1081 catgatcctt cagaagcgaa accagacagg atgtgccaaa agccgccgac ggccggagac
     1141 gctggccggg gcgacgcttc gcacgggcgc cgcggcgaga ggggccggcc tgccgggccc
     1201 cttccgtggc ggcttgccgg gcctgatagc cgccgaagcc gcgctttgcc aacagcgatt
     1261 ttgacggagg cgtgacaggg cggcggactc cacgctcaga ggcggcggcg gcgaggctcg
     1321 gccggccggc tttgaaccgc gggcgtcgcc ggaggttgag accggcagcg aagccaggag
     1381 acacggatat ggaggaccga aagcaacgcc ccgaggccga ggacgagacc cggccgcagc
     1441 gtggcggcga ggccgcgacc acccggttcg gcatggagga cggcgacaat accgacgttc
     1501 ccgcgaccgg cggcccggcc gcggacgagg cgggagagca ggacggcggc ggctagccct
     1561 tctcccgctt gccggagaag ggctctgacg gccagcttcc ggaggagctt acccaaggcc
     1621 gcagcgctgc acgggcagtt ctagcagaaa gaaaaagacc ctcaccctag ccctctcccg
     1681 cgagcgggag agggggaatt catgcccctg cgaaggcagg ggcccagacc gggcgaagcc
     1741 cgccagccaa gctctcgtag aaatcgcgag tctggggccg tgtgggctac ggcgccaagc
     1801 tcccacagcc gctgcgatga ccgggccgcc gccgaagcgg caacccggac ccgggcccct
     1861 gccttcgcag gggcaccatg ctctcgcttc tgatgggggc ccgcctccgg cccagctacg
     1921 atgcgagcgg aagcggtcac gatcgcaggc ttgcgaccgt tcgtgcttcc gcccggttgc
     1981 ccgaaccggc gcccgccccc attcagggat tcgggccgcc agtccgctgg atcagcaatt
     2041 gccctcgcgt ccgtccacgc aatccccgac cgattcgacg tcgtcggcgg ctccgcgaac
     2101 cgtgccgcag gctgcgagga tgagactgct cgagatcgca acgatggcca gaatcttacg
     2161 catgatatcg tctcctaagg cctgaacgcg cagtgcccaa atgtcgggat gcgaggcgaa
     2221 agctagaatg tcttgcaggc gggcttggtt ccacctgcgt cacgacccgg ttagatagaa
     2281 ttctaacctt ccaacgcttc cccaggagcg accatggagc acggctattc aggaacgccc
     2341 ctcgccagga agctgggcgt gagcgccgga caaaatgcgt ggcaggccgg gatgcccgcc
     2401 tcggttcgca gccggatcga ggccgatatt cccggcttga ggttcgccga gtcgatcgag
     2461 cctccgctcg actttgccca cctgttcacc aagtcgcggg cgcatctgga ggagcagctt
     2521 gtccggcttc gaagcgcgct cgctccggcc ggcatgattt gggtgtcctg gccgaagaag
     2581 gcatcgaacg tggcgaccga catcaccgaa gacacgatac gcgaggtcgc gctgccgctt
     2641 ggcctggtcg acgtcaaagt ctgtgcggtc gacgagacct ggtcgggact gaagctggtg
     2701 atccgccgat ccttgcgctg accccctgat cgcgcccggt tacacgccct gatccacgtc
     2761 gtcgcttgcg ttgaaggagc gccggacgat ctcctccagc acgggcatgt ccacgtcggc
     2821 cagcttgttg acgtaaaggc acgcctgcga ccgtttgtgc tttccgagct ttccaagcag
     2881 cgcctcatat tcgggaaaac cgccgacata gaggacgagg ttcgccgagc gcggcgagaa
     2941 accgacccgg cacatgtcgc cctcgcgccc gctgtcgtgg cgatagtggc aggttccgaa
     3001 gccgacgatc gtcggccccc atatcgttgc cggctcgccg gtcacgcgct ccatcatgtc
     3061 gcgcaggact tcggcgtcgg cgcggcgccc cggctttgcg accgccgcca tgaactcatc
     3121 gacattcgct tgcgtcggct tggtttttat ctccgccatg gacatccctt cgagtcgttg
     3181 ccgcagctat cgtggtcgaa agcgggtatt aggcaaatcg tccgcactcc cggccggttg
     3241 cgccgcatat ctccagggcg cacaagccat tccgcttacc atcgggagaa ggtacatgga
     3301 cactgcatcc cctgcatcgc ctccgcgatg gtttcgggca gtcgcgctgc tggccgtgat
     3361 ctggaacgcg gtcggcgtcg ccacgtattt gagctcggtc ggcctgttcg gcgatccgat
     3421 ggcggggctc agcgagaccg aacgggcggc ggcctcgtcg atgcccggct ggatcacggg
     3481 cctgttcgca accggcacct tcgccggcct cgccggcagc gtggggctcc tcctccgcaa
     3541 ggcctgggcg ctgccggtgc tgatcgtctc gctggtggcg ttgctggtgc tggaggggtg
     3601 ggtcctattc ttgagcgacg cggtggagcg gttcggcgtc gcggtgccgg tcatggtcag
     3661 cgcgggggcg atcctgctgg catggctggc gagccacgcg cgccaacgag gctggttgtc
     3721 gtgagcctgt tctagcgccg gcgatcagcg accatgcgcc ttgccctttt cgagcccgac
     3781 caggccggca atgtcggcac catcctgcgg ctcggcgcgt gcctcggcgt gccggtcgac
     3841 gtcatcgagc cgtgcgggtt tccgtggagc gacaaggcgc tgaagcgggc cggcatggac
     3901 tatgccgcga tggcgaacgt cacccgccat gctgattggg cggcattcgg ggcgcggctg
     3961 gcgggccggc tggcgctgtt cacgaccgcc ggcgcggtgc ggctgccgga ggcgcggttc
     4021 gagcccggcg acaccttgct gttcggctcc gaaagccgcg gcgcgccgga ggcggtgcac
     4081 gagcgggcgg acctgcgggt gcggatcccg caggccgagg gcacgcgctc attgaatttg
     4141 gcggtggcgg cggggatcgg cgtcgccgag gcgctgcggc agacgcattt gtgggccgga
     4201 tgattcggag gcgcaaggag gagggcaatc ggttcggtgc gggagaagaa ggtggggcgc
     4261 gcgaagaggc gcggaggcgc ggcgatatcc atgagcgctt ggctgacggc ccctgccttc
     4321 gcaggggcac aagaacgcat gcgccgcgcg ccgtggacat cgccggccac gccgtcgtgc
     4381 cgagcatcga aacggtcctc ccggccgggc gagagcgtcg cccggccttt tcctcagacc
     4441 acttcgaaat tggtatggga gagcgcaagc ccggcatcga ccctcgccaa cagcacggcc
     4501 gcgccggagc cgttgccgtc cgcgtcgaac cagatgttcc cattcgcctt gttgtagatg
     4561 atccggtcgt cggcatcctg tgccgccgtc cccgttcgga aggccccggc ggccagcgag
     4621 ccggccgggc cggcggggat gaaatcgttc cagagaacga tcttttcgct gccggcgtgg
     4681 aagtcggtga tccggtcgac ctgtccatcc gggacgtaag agaaggagaa tttgtcggtc
     4741 cccgtgccgc cggtcagcac gtccgaaccg gcgcggcccg acaaccagtc ctcgccggca
     4801 ccgccgacga gaacgtcttc cccggcgctc ccgcccatat aattgtcgag cccgttaccg
     4861 gtgaatttca cgtcccgcga ccaggcgaag caatcctcga cgttggcggc caacacatag
     4921 gtgccgacgg cataaacggc gtcatggccg ccgcccgccc gctcgaccac cgtatccgcg
     4981 cccgcggcgt ttacattcat gtaaatgtcg tcgcccgcgc cgccgatcag ggtgtcttca
     5041 ccacccttgc cgtcgagggc gttggtgcgg ccgttaccga cgatccggtt cgagaggccg
     5101 ttgccgtcgg cgtcgacgtc ttcgttcccg aacatcgtca ggttctcgac gttgagcggg
     5161 agatcgccct gcaaacggac gtcggtgagg atcgtgtcga tcccctggcc cgccgcttcg
     5221 atgatgatcg tgtccgaaga tcccgacgtg ttcgggatca tgtgaaggac gtaggtgtcg
     5281 tttcctgctc ctccctccag gatgtcgttg ccgccgtgcg catcctcggc cggaactccg
     5341 ccgtcgagaa cgttgtcggc ggagttgccg acgatgtggt tgaaaaggct gttcccggtg
     5401 ccgtcgatcg cgcgcgcgcc gaggagcgtg agattctcca cgttcgcggc gaggcggtgc
     5461 gaaaccctgc tctcgaccgt atccgaacca ccgtcggcgg cctcgcggac gatgtcgccc
     5521 agatcgtcca cgaggtagcg atcgtcgccg tcgccgccgc tcatccgatc cgcgccgccc
     5581 cggccgtcga gccggttggc ggccgaattg ccgacgatga ggttggccag gccgttcccg
     5641 gtgccgtcga gcgcgccgcc gaccagaagg agcgtctcca cgttcgcggt cagcgtgtag
     5701 cttatcgccg cgcggaccgt gtccctgccc gcgtcgagcg cctctacgac ccggtcgccg
     5761 gccgtcgcga ggacataggt gtcgttgccg aggccgccgt tcagccggtc gaacccgaga
     5821 ccgccgtcga ggcgatcgtc gccgtcgcct ccatcgagcg tgtcgtcgcc gccgcggccg
     5881 tagatccagt cgtcacccgc ctcgccggcc agaatttcgg cttgattggt gccgaccagc
     5941 cggtcgttcc cgtcggtggc cgccgccgcc agcctggcgt tgctccgctt caaacgcatt
     6001 ccatcctccg ccacccggcg gtccgccgaa aaatccacga tcattattcc ccccgatcga
     6061 gcgccctggc gcccgttgcg gcaggaatag cggctatcgc cctcaagaca agcaagttct
     6121 tgccggcgcg gacggaagga attgccggcc tgattctaga ggacccggat tctcggcggc
     6181 gcccctcccc ggcaaaggcc ggggaggaat cggcggtgct ctccttacct gcgtcgcgcg
     6241 ggcgattgct ttcgcggctt cgccggcgct acctcgtggc gaggagggcc aggccgtgcg
     6301 gacactcacc aacatcctcg gtgctgccgc gctgctctgc gccatggcgc cggccgccgc
     6361 gccggcggct cagccattgc cccaaaatcc cgcctccgcc gaggcatcga tcgacggcct
     6421 ctggcgcggc gaggtcggcg gccggacggt gctggcctgc ttcaacgcga cgatcggctc
     6481 cttctacgcg ccggccgacc ggaagctcat catgctgtgg gccgaacagc cgctgcgctg
     6541 ggcggaaaag gacgaacgcg atcccgccaa tccacgctgg aacctgcgct tgcagggcaa
     6601 tgaccgcctc gccggggagc gggccgccgg ccgcgacgcg caggcgctga cgcttgcccg
     6661 gctgcccgcc cccgctgcgg aagatgccga tagcgatgcc acaggtccgt gcaggagcct
     6721 cgcttatcat cgcgaacggt tggagccgct cgcggtgcgg gagcggccgg cgaggctcgg
     6781 caccgccggc tatgtcgaga tcgtcgccgc gcccgcttcc tatccggacg cggtccgcta
     6841 ccaaagcttc cgagttccgg gggacgcgcc cggcaccgcc gcggtcaacg ccctgctcgc
     6901 ccgcatgatc gatcttcgcg ggccggggga aagcgaatgg agcctgtgcc ttgccgccgc
     6961 gggcaatagc ggcggcgact tccacgccgg gctggcgccg gtccgcatcg gccgccgctt
     7021 cgtcgccgcc gaggaggagc gcgaggcaag ctgcggcggc gcccaccctt cttccggcag
     7081 ctacccccgc ctgttcgacc tcgtcgaagg ccgagaggtg gcgatgcgag actggctcaa
     7141 tccgcgcgcg ctcgacggcg agctgctgga cgaacgaagc gagccgaacc ccgatgccga
     7201 gccgaacgtg cttccgctgt cggctgcgct ccgccgcgtc ctcgtcagca aggccggaaa
     7261 gcccgagccc gagtgcgccg gcacggtcga cgaggagact tactggacag tcggcgtcgg
     7321 ccgcgatgcc ctcctcttct acccccatct agcccgcgcg gtgcgtgcct gcggggagga
     7381 aatcgccctt cccttcacgg cgatggcgcc gttcctcagc gcagagggga agacggcgct
     7441 cgcccgcctc cgcgccgatc ccgccgtttt cgcccagcct tagcgcattg cttgcatcgc
     7501 ccgccgccgt gcggcaatcg gccacttcat gatcgagatg gacgaacagc agcaggcggc
     7561 gcgcgcctgg ttcgaaagct tgcgtgagcg gatttgcgcg gaattcgaag cgatcgagcg
     7621 cgaggcgggg agcgccgcgg cgttcgaata tctgccctgg gaccggctcg acccggacgg
     7681 ttcgcccggc ggcggcgggg tgcgcgggct gatgaagggc caggtgttcg agaaggtcgg
     7741 cgtcaacgtt tcgacggtgg gcggccggtt cagcccggaa ttcgcgcgca ccatccacgg
     7801 cgccgaggag gatcccagct tcttcgcgac cggcatcagc ctggtcgccc atatggccaa
     7861 cccgcacgtg ccggcggtgc acatgaacac ccgcttcctg gtcaccacca agagctggtt
     7921 cggcggcggc gccgacctta atccgccgct cccttacgag gaagacaccg ccgatttcca
     7981 cgcccggctg cgcgccgcct gcgccgccca cgacccgact tactatccgc gcttctcgaa
     8041 atgggccgag gattatttct tcatcccgca ccgcggggtg agccgcgggg tgggcggcat
     8101 attctacgat catctcgaat gcgctgagcg cggccagggc gcgggcttcg atgagcattt
     8161 cgccttcacc cgcgacgtcg gcgaggcgtt cctcgacatc ttcccgcgca tcgtccggcg
     8221 gcggatgaac acgccttttt cggccgagga caaggcgcgg cagctcgaat ggcgcggccg
     8281 ctatgccgag ttcaacctga tctacgaccg cggcaccctg ttcggcctca ggaccggcgg
     8341 caatgtcgac gcgatcctga tgagcctgcc cccgctcgcc acctgggggt gaggcgcggc
     8401 tagtgcattg aagggtaagt tgtccctgtt gccgttcggg ctgagccttt cgtctttcgc
     8461 ctcggaacaa gaagaaagcc ccgacccgcg cgatgcctga gcgcgctcgg ctggcggccc
     8521 ctgccttcgc gggggcacag gaacggcaaa ccccaggtgc ccctgcgaag gcaggggccc
     8581 agacccgggt tgccgcttca gcggcagtcc ggttatcgca acggccgtgg gagctcggcg
     8641 ccgcagcccg cagggttccc ttcgacaggg ctcagggcga acggatgatg gtgattccat
     8701 tcgaccttca acgctctaag cgctgctcag gcggcttcgg ggcgctccgg aaccaggatc
     8761 agcgtgcgtc cctcgacccg ccaggaggcg agcttcgaga ggaagttcat gccgagcacg
     8821 tcgagatcgc cgagcgcggg cgtgatcacg atcttgaggt cgctggcgac gatgtcgccg
     8881 acccggagct cccggacgct gccggtgcgg gcgcgggcga gtccgttggc ggtcttgagc
     8941 acgaccggca cgagcgtagc gtcgtgggcg atgccgctgg cccgcgccgt ctgctcggaa
     9001 atggcggtga tggtggcgcc gctgtcgatc atcatcctat gcttggtgtc gttgagggtg
     9061 gcgttggccc agaaatggcc gtccggcgcc atcggcaccc ttaccgcgcc gccgaccacc
     9121 tcttgccggt cgaggccgag ccggtcggcc agcggcgcga gcattggctc atagggcgcg
     9181 tgctgcagga gcaggaacag gcacagagcg agcagcccga aggagaggcc gaaacgcacg
     9241 atccggccga tcaccggcac ccgctggagc aggatcagca gcagcgccgc tccgcctgcg
     9301 tagagggcga gctgctgcca gtcggagctg atgtcggtcg gcatggctgg ctgattaacc
     9361 cctcacgtgc ccgcctgcct tcgcaggggc accaggaacg gccaacccca agcctgcacc
     9421 tggaagctat tgcgtgcagg ctgccgggcg ggtgccggcg ggctggccga ggcgcggcgc
     9481 cgggcaggcg ggagcgggag ccagacgctg cggcatggcc gcgcgcagcg gctgggtcgc
     9541 cggcatccaa gccggccgtt tcggcgtctg cgcctcggcg ccgaacgcgt cgtaaatggc
     9601 gcgggcggcg gtggcgatca cggccggccg gttcgatccg ccgcgggcga agaaggcgac
     9661 cgcgatgcgc tggccggtgg gcgtcgtcag gaagccgacg tcgccggtat aattgttcag
     9721 cgtgccggtc ttgttctcga cccgcgcgcc ggccgggaga atgccgcgga tgcggttcga
     9781 gccggtcttg cagcgccgca tcatgtcgag cagcagcacg cggctgtcgg ccttgagcgc
     9841 gacgcccgcg tcgatcaccc gcagcaggcc gagcatcgcc gccggcgtgc tcgaatcgcg
     9901 caggtcgctg aggtcgcggc ggtcgccgag cagctgggcg atggtgcggt cgacgcggat
     9961 gccgctgaga ttgtgggcgc gcagccagcg gtcgatcgag gccggcccgc cgagcgtcgc
    10021 catcaggagg tcggtcgcgc gattgtcgct gcgcgtgatc atcgcgtcca tcagccggcc
    10081 cgcgctcgcg cccgcgacgg tgtcgtcgag gctgcgccgg ccggcgtcga cctcggccag
    10141 gtaagcggcc gcgaccgcga ccttcatcgt gctcgccatc gggaacggcc ggtcgccgtt
    10201 gaagctgatc gtcgccccgg tcgcgaggtc gagcgcggca atgccatatt cgccgggatt
    10261 ttcgatggcc agcgccgcga gccgctgctc gagcgagcgc agggtcggcg agacttcggc
    10321 gagggccggc tgggcggcaa aggcgaggag ccaggcaagc aaagcaacca aacgcatcgt
    10381 aagcaccccc caaatcgggc ccgcggcgcc ccacccgctg ggtcgcccgg ccgctgactc
    10441 gcaggatagg gctttccgcc gcggataata ctaacccgtt tgactatagg gactcgttcg
    10501 tcgctcttcg cgcccgctcc gccgcaggag gtcccgaatc gaggccctgc ccgtcattcc
    10561 agcgaaagct ggaatctcac ttttcttggc tcggctgtcg aaggaaaaaa gggagatccc
    10621 agctttgctg ggataacgat cgtgcgggtg gcggcagcaa ccgcttgact ttcgccctgg
    10681 cctccctcat ttcgacttat atcgaagcga ggtcgcgaat ggactcaccc tctgcagtct
    10741 cagcactcgg cgcgctcgcg caggagcatc ggctcgcttt gttccggctg ctcgtccggg
    10801 ccggcggcga gggcatggca gcgggcgcga tcgcggaagc cttggcggtc ccgaattctt
    10861 cgctctcctt ccacctcgcg cagctccaca aagccgggct ggtgcgccag gaaaggcggc
    10921 accgctcgat catctaccgc gccgactatg cggtgatgaa cgacctcgtc ggctatctca
    10981 tggagaattg ctgcgccgga gccgattgcg gcgccgcccc ggagtgcggc gccggcaccg
    11041 gtccagccca gctcgaaagg acagacatat gaagcgcctg cacgttcacg tcggggtcga
    11101 gaatctcgac cgctccgtct ccttctactc gaccttgttc ggcgccggcc ccagcgtcct
    11161 caagggcgat tatgccaaat ggatgctcga cgatccgcgg gtgaatttcg ccatctccgc
    11221 cggcaaccat gccgccaaag gcgtcgagca tctcggcatc caggccgaaa gcgaggacga
    11281 gctcgccgaa gtctatggcc gcctcaaggc cgccgaccgg ccaatgctgg aggaaggcgc
    11341 aaccacctgc tgctacgccc gatcggagaa gagctggatc gccgatcccg acggggtcgt
    11401 ctgggaggcc ttcctcacca acggcgaagc gaccgtctac ggcgacagcc ccgcgctcgc
    11461 ggcgctggcg gagaaggatg cggaaacatc aggctgctgc gacgcggcca cgccgcaggg
    11521 cgtctgctgc gaacccaagc cgcagcttgc cgccgacgcc ccctgctgcg gctgatggcg
    11581 gcccagcctg acgcggcgac gctgacggac gcggccccgc cgcgtccggg catgacgcgg
    11641 ttcgagcgct atctgtcgat ctgggtcgcg ctctgcatcg ccgccggcat cgccctcggc
    11701 gccgccgcgc ccggcctgtt cgcgctgatc gcgtcagccg agctcgcccg cgtcaacctg
    11761 ccggtggcgt tgctggtctg gctgatgatc gtgccgatgc tgctcaagat cgacttcgcc
    11821 gcgctcgggc aggtgcgcca acatatgcgg ggcgtcggcg tcaccttgtt catcaactgg
    11881 gcggtgaagc ccttctcgat ggcgttgctc ggcgcgatct tcgtcggctg gctgttccgg
    11941 cccctgctcc cggccgacca gatcgacagc tacattgccg ggctgatcct gctcgccgcg
    12001 gcgccgtgca ccgcgatggt gttcgtctgg tcgaacctgt gcgagggcga gccgcattat
    12061 acgctcagcc aggtcgcgct gaacgacgtc atcatgctgt tcgccttcgc gccgctggtc
    12121 gggctgctgc tcggcgtcgc ctcgatcgcg gtgccgtggg acacgttgct gctgtcggtg
    12181 ctgctctaca tcctggtgcc ggtggtcgtc gcccagctga tccggcgccg gcgcctcgcc
    12241 gtcggcggcc agccggcgct cgacgccttg ctccgccgcc tcggcccggt gtcgctggcc
    12301 gctttgctca ccacgttggt gctgctgttc ggcttccagg gcgaagcgat cctcgcgcag
    12361 ccgctggtca tcgccctgct cgccgtgccg atcctcatcc aggtctattt caacgccggc
    12421 ctcgcttatt ggctgagccg ccgcttcggc gtggcctggt gcgtggcggc gccggccgcc
    12481 ttgatcggcg ccagcaactt cttcgagctc gccgtcgccg ccgcgatcag cctgttcggc
    12541 ctcaagtccg gcgcggccct ggccacggtc gtcggcgtat tggtggaggt gccggtgatg
    12601 ttgtcggtgg tggcaatcgt caagcgcacg cgaggctggt acgaacgcgc cgatgcggtg
    12661 tcgtgaggtt cacgcccttg ttgcggagtg catgcgggtg agcgagaagc ccctccacca
    12721 cccggctttg ccgggcggtc cccctccccc aggcgagctg ggggaggatt gagatgccgc
    12781 tgacggaggt tccccctggc catgtcggcg cggtcgtcac ttatctcgag atgacggtcc
    12841 gccccctgcc ccggccgatt cccgcctcgc cgctcaggct ggcggcgtgg cggccgcttg
    12901 tcctcggcaa atatcgcgcg ctgttcaggc gtgtcggggc gccgtggctg tggttttcaa
    12961 ggctcgcaat ggacgatgcg gcgctcgccg agcgcaccgg cgaggtctgg gcggtcgagg
    13021 atgcggacgg aaacgaggtc ggcatcgtcg agctcgattt ccgcgcgccc ggcgaatgcc
    13081 tgatcgcgtt tctcgggctg gtcccggagc ttgccggcaa aggccatggc tcgtggctgt
    13141 tcggcgaggt gctggcgctg gcctggcgcc ccggcgtcga gcgggtgcat gtccatacct
    13201 gcacgctcga tcatccggcg gcgctgaagg cgtatctgaa ggccggcttc accgcctatc
    13261 agcgcgcctt cgaaagcttt gccgatccgc ggctgctcgg gctgctgccg cggggcgcgg
    13321 cgcctcagat cccgctggtc ggccccgacg cctccgccag ccgccggtag agcgaagcga
    13381 gcaggaccat cagcaccgta tagaaggcgg cgccgagcgc ggtgctgaag atcagcagca
    13441 gcatctcgcc cagcccctcg gcccccgcgg cgcggccgat cagcaggaaa atggagccga
    13501 gcacgtagac gccggcgagg ctcagcacga aataagcgag gatcagcacc agcagcaaag
    13561 cgaggatcgc ccaaccattg cccttggtga ccgcgacgct gcgccgcaga gcatcgaccg
    13621 ggttgcggag cctctccgcc acgatcgccg gccccgccac cgccagccgg gccatcagat
    13681 agaggccggg gacgatgaac aggaccagcc cgacgaagat gaccaggccg gagaggaagg
    13741 aggtcaggaa atagaccggc agcagcaaag ccgccgcctt gatcgcgccg ccgacggtcg
    13801 gatcgtcccg gtccaaagtg aggatgaaga gggcgagatt gccaatgagg ccgatcaaat
    13861 tgaccagcag cagcagcggc cagttcgcgc gcatatgctg catcagcatc tccagcgtcg
    13921 gaccgtcgcg gccgccggtc ggctcgggaa cgaaatagcc gaacgccagc gtcggcagga
    13981 acaggaacac ccccgccacc gccgccacca gcgacgcgtt ggcggccagc atctgccttg
    14041 tgtcgtccca gaccgcgctc atcgaaaact tcatgccttg gtccccttcg ccgggaaacg
    14101 cgcttcccgc cccgctccgc ggagccgcca tagccgcgcc cgggcgaggc tatcaagcga
    14161 gccctaacgg cggggcggcg ttctgagtgc ccctgcgaag gcaggggccg ccagccaagc
    14221 tctcgtggac atcgcccaag cccggaaccg tgcgagctac cgcgccaggc ttccacagcc
    14281 gctgcgatga ccaggctgcc gctgaagcgg caacccgggg ctgggcccct gccttcgcag
    14341 gggcatttgg cgcaggcttg gggtttgccg ttcttgtgcc cctgcgaagg caggggccca
    14401 gcccggcccc tgcgaaggca ggggccgcca gccaagctct cgtggatatc gcgtagcccg
    14461 gaaccgtgcg tgctacggcg ccaggcttcc acagccgctg cgatgac
//
LOCUS       DASVRK010000206         9658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_227444, whole genome shotgun sequence.
ACCESSION   DASVRK010000206 DASVRK010000000
VERSION     DASVRK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227444"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="VF589_08110"
     CDS             complement(<1..144)
                     /locus_tag="VF589_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257584.1"
                     /translation="MATAPTTNYTGRISQIIGAVVDVSFDGNLPAILNALETDNNGAR
                     LVLE"
     gene            complement(232..1119)
                     /locus_tag="VF589_08115"
     CDS             complement(232..1119)
                     /locus_tag="VF589_08115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX8257585.1"
                     /translation="MASLKALKGRIASVKSTQKITKAMKMVAAAKLRRAQQAAEAGRP
                     YAERLVAVMASLASRVTVGPESPKLLAGTGSDQTHLLVVCTSDRGLAGAFNSNIVRAA
                     RKKADDLTRQGKTVYFYLVGRKGRAVIGRLFPKQIIHQYDTSAIKQVSFGDAAAIAAE
                     ITEMFANGRFDVAHLFYARFQSALVQEPMALQIIPVPVAVAGSAGDQAGASAAVEYEP
                     EEEEILAALLPRNVAVQIFRAMLENAASEQGSRMTAMDNATRNAGDMINRLSIQYNRT
                     RQAAITTELVEIISGAEAL"
     gene            complement(1193..2722)
                     /gene="atpA"
                     /locus_tag="VF589_08120"
     CDS             complement(1193..2722)
                     /gene="atpA"
                     /locus_tag="VF589_08120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX8257586.1"
                     /translation="MDIRAAEISKVIRDQIASFGTEAQVSEVGTVLSVGDGIARIHGL
                     DNVQAGEMIAFPNGIQGMALNLEADNVGAVIFGNDSAIKEGDTVSRTGTIVDVPVGKG
                     LLGRVVDGLGNPIDGKGPIETTERRRVETKAPGIIPRKSVHEPVQTGLKALDALVPVG
                     RGQRELIIGDRQTGKSAVALDTFINQRTANAGEDESQKLYCIYVAIGQKRSTVAQIVR
                     ALEENGAMEYSIVVAATASEPAPLQFLAPYTGCTMGEYFRDNGMHAVIVYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNEANGSGSLTALPIIETQAGDV
                     SAYIPTNVISITDGQIFLETELFYQGIRPAINVGLSVSRVGSAAQTKAMKKVAGSIKL
                     ELAQYREMAAFAQFGSDLDASTQRLLNRGARLTELLKQPQFQPMPMEEQVVSIFAGVN
                     GFIDTIPTKEVVRFESALLSHVRSDHPQVLAKIRDTKALDDETTGQLKTIVGDFAKAF
                     G"
     gene            complement(2725..3279)
                     /locus_tag="VF589_08125"
     CDS             complement(2725..3279)
                     /locus_tag="VF589_08125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX8257587.1"
                     /translation="MEHSGGIQASLAGRYATALFELARDHKRLDAVAESLAALRAALA
                     QSPELKELTASPLIGRDEATRAILAAAGSMGIDPLTTNFLGVLARNRRLGQLPQVIRA
                     FNMLAANHRGEITAEVTSAHALDGEQVTALKSNLKSRLGRDVAVDLTVDPTILGGLVV
                     KVGSQMIDGSIRTKLNNLAMAMKG"
     gene            complement(3419..3850)
                     /locus_tag="VF589_08130"
     CDS             complement(3419..3850)
                     /locus_tag="VF589_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257588.1"
                     /translation="MRIVILAALLALSGCGRAAADGDGNGGAPITAAAPANSSAAAPA
                     GGTFPPDFPAADATSNGSACYLYLMIAAGAADAATGFDAPAMEQAAGQWRALLLQTMA
                     EEEVRQFTAGETNFRLQTPPAERNAAARWCVENAPEVDPAG"
     gene            complement(3865..4314)
                     /locus_tag="VF589_08135"
     CDS             complement(3865..4314)
                     /locus_tag="VF589_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257589.1"
                     /translation="MINSFPKPLLAAALLAIAPLAAAAAYAPEVSATAAAAEGSAQRR
                     AILDALRPGVVKAMRGPVEFVVERLEVKDGWALVLAQPQRPGGGRIDPASVLPADQLE
                     FTDGLRIDALLRFKGGKWVPEDFKVGATDVWYVCYPGAPKALTGCGD"
     gene            4511..4639
                     /locus_tag="VF589_08140"
     CDS             4511..4639
                     /locus_tag="VF589_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257590.1"
                     /translation="MTYRIEGLPENPLPDLPELSEAATIHPRNSAHGCLAARVERH"
     gene            4651..5727
                     /gene="ada"
                     /locus_tag="VF589_08145"
     CDS             4651..5727
                     /gene="ada"
                     /locus_tag="VF589_08145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEX8257591.1"
                     /translation="MMRAEAGTIDEDWAWARAFARDRTADGLFVMGVLTTGIYCRPSC
                     AARHPRRENVRFFACGADAAATGLRACLRCKPDDVTREAAALERAYRLLEAAEEPPAL
                     AALAAAAGYSPHHFHRLFKRATGVTPAQYARGRRAGEMARSLAKEERVTDAIYAAGYS
                     GPGRFYADAGARLGMTPSAWRDGGRGETIRWTTAQTDLGAMLVAATAKGICRLSFDEG
                     EAELRRRFPNARIEPGGSEMAELAERAVAAVNAPERPHDLPLDVRGTAFQEAVWRELS
                     RIPPGESLSYAALAARAGKPGAARAAGSACGANSVAVLIPCHRARRGDGSLGGFAYGL
                     DRKTALLKRERGLKRERGLERERG"
     gene            complement(5734..7266)
                     /locus_tag="VF589_08150"
     CDS             complement(5734..7266)
                     /locus_tag="VF589_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8257592.1"
                     /translation="MLCRLLLAMIALFAAVPAGAAWREASSDHFVIYSEDSEAALRKF
                     ATRLERYDAAMRLMYGVPDTDMGPASRLTVYAVGNLKAVQRLAPGNDNFIIGFYIPRA
                     GGSVAFIPRRLSSDASFLRAETVLLHEYAHHFLYQNHAAAYPAWFSEGFAEFNATARF
                     EEDGSVGLGVPAEHRAYSLFAGDPLPLDKMMGQLPSRMNSHQREALYGRGWVLTHFLT
                     FEPSRKGQLANYLTALNSGKGGVEAATAAFGDLGKLSGELDGYVKRQRLPYTRVNADD
                     SMIGKIAIRSLSPGEAAMMDVRIVSRRGVNLKQAKALVPEARRAAAPFPNDPAVQTWL
                     AETEYDAGYYAEAEAAADRALAVDPKSIEALIYKGRVKMAVAEDKKATDAATWKEVRR
                     WLAAANRADPDDPEPLILFYSSFGAQGVKPTANAVVGLNQAYALAPQDNDLRFMAAYQ
                     FLVDRKAREARVALAPIAFNPHATEAGKLAAAMIEQIDTGNIDGALKAGEAPAKEKAA
                     GT"
     gene            7426..7611
                     /locus_tag="VF589_08155"
     CDS             7426..7611
                     /locus_tag="VF589_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257593.1"
                     /translation="MDDKDRRPATDPPARGSKIVWIIGAILLLLVLFLGIRGCDYASR
                     ETNYQESANGTVDAPDV"
     gene            complement(7626..>9658)
                     /locus_tag="VF589_08160"
     CDS             complement(7626..>9658)
                     /locus_tag="VF589_08160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX8257594.1"
                     /translation="WEPDRLETAEIEEARLRPLLHIYDAPPIGAPLRRLIEWTADYYL
                     APVASVLRMALPSSSALDGARTVTEYRATGMVPERLTPQREQALERIAGRQGLVRELA
                     LAADVSDGVIRGLIKSGAIEALTVSVDDPFPRPDPDYHPPRLEPAQRSAARAFVDAVQ
                     AAEFAPFLLDGVTGSGKTEVYFEAVAENIRMGRQTLVLLPEIALTEPFLKRFALRFGC
                     EPVAWHSGLRQSQRRRAWRSIATGEARVVVGARSALFLPYPNLGLIVVDEAHETSFKQ
                     EEGVQYHARDVAVMRAKLEKVPVILASATPAIETRQMAEIGVYAEVKLPDRYGAAEMP
                     DIAAIDLIQDPPPRGRWLAPTLVRELETNLERGEQSLLFLNRRGYAPLTLCRACGHRF
                     QCPNCTAWMVEHRLVHRLACHHCGHMMPPPRACPECKEEDSLVPVGPGVERIADEVAA
                     LFPEARTAIVTSDTIWSPAKAAEFVGRMEAGAIDIVIGTQLVTKGYHFPNLTLVGVVD
                     ADLGLSGGDLRAAERSFQQISQVSGRAGRGEKPGRVLVQTHEPGAPVISALVGGDAEG
                     FYAAETQARREAAMPPFGRLAGIIVSAEDLGEATETARLIGRTAPKVENMAVFGPAPA
                     PLAMLRGRHRQRLLVHAARSLDVQEVIRDWLGALDWPRGVRVAVDVDPYSFL"
BASE COUNT         1455 a         3195 c         3445 g         1563 t
ORIGIN      
        1 ctcgaggacc aggcgggcgc cgttgttgtc ggtctcgagg gcgttcagga ttgccgggag
       61 gttgccatcg aacgacacgt cgacgacggc gccgatgatc tggctgatgc ggccggtgta
      121 gttggtggtg ggggcggttg ccattgctgt attccctgcc ttctttcctt gtgtccccgc
      181 gaaggcgggg acccagactg ggctcctgcc ttcgcaggag cacaaaaacg tttagagcgc
      241 ctcggcgccc gagatgattt caaccagttc ggtggtgatc gccgcctgcc gcgtccggtt
      301 atactggatc gagaggcggt tgatcatgtc gccggcgttg cgcgtcgcat tgtccatcgc
      361 ggtcatgcgg ctgccctgct cggacgcggc gttttcgagc atcgcgcgga agatctggac
      421 ggcgacgttg cgcggcagga gcgcggcgag gatttcctcc tcctcgggct catattccac
      481 ggcggcgctg gcgccggcct ggtcgcccgc cgaaccggcg acggccaccg gaaccgggat
      541 gatctggagc gccatcggct cctgcaccag cgccgactgg aagcgggcgt agaacaggtg
      601 ggcgacatcg aagcggccgt tggcgaacat ctcggtgatc tcggcggcga tcgcggcggc
      661 gtcgccgaag ctcacctgct tgatcgcgct ggtgtcatat tgatggatga tctgcttcgg
      721 gaacagccgg ccgatcacag cgcgtccctt gcgcccgacc agatagaaat agaccgtctt
      781 gccctggcgg gtgaggtcgt cggccttctt acgggcggcg cgcacgatgt tggagttgaa
      841 cgcgccggca aggccgcggt ccgacgtgca gacgaccagc aggtgcgtct ggtcgctgcc
      901 ggtgccggcg agcagcttcg ggctctcggg gccgaccgtg acccggctgg cgaggctcgc
      961 catcaccgcc accaggcgct cggcataggg acgcccggcc tcggccgcct gctgggcgcg
     1021 cctgagcttg gcggcggcca ccatcttcat cgccttggtg atcttctggg tcgacttgac
     1081 cgaggcgatc cgtcccttga gtgccttgag agaggccatt tagctttcct cttgtgctcc
     1141 cgcggaggcg ggagcccagt ctgggttcct gccttcgcag gaacacatgt gcttatccaa
     1201 atgccttcgc gaaatcgccg acgatggtct tgagctgccc ggtcgtctcg tcgtcgagcg
     1261 ccttggtgtc gcggatcttg gccagcacct gcgggtgatc gctgcgcacg tggctgagca
     1321 aagcgctctc gaaccgcacg acctccttgg tcgggatggt gtcgatgaac ccgttgacgc
     1381 cggcgaagat cgagacgacc tgctcttcca tcggcatcgg ctggaattgc ggctgcttca
     1441 acagctcggt gagccgcgcg ccgcggttga ggaggcgctg ggtcgaggcg tcgaggtcgg
     1501 agccgaactg ggcgaaggcc gccatctcgc gatactgggc gagctcgagc ttgatcgagc
     1561 cggcgacctt cttcatcgcc ttggtctggg cggcggagcc gactcgcgac accgagaggc
     1621 cgacgttgat ggccgggcgg atgccctggt agaacagctc ggtctccagg aagatctggc
     1681 cgtcggtgat cgagatgacg ttggtcggga tgtaggccga aacgtcgccc gcctgggtct
     1741 cgatgatcgg aagcgcggtc agcgagccgg agccgttggc ctcgttcatc ttggccgcgc
     1801 gctcgagaag cctcgagtgg aggtagaaga cgtcgcccgg ataggcctcg cggcccggcg
     1861 ggcggcgaag cagaagcgac atctggcgat aggccacggc ctgcttggaa aggtcgtcgt
     1921 agacgatgac ggcatgcatt ccgttgtcgc ggaaatattc gcccatcgtg cagccggtgt
     1981 agggcgccag gaactggagc ggggcgggtt cggaggcggt ggcggcgacg acgatgctgt
     2041 actccattgc gccattctct tcgagcgcgc ggacgatctg agcgaccgtg gagcgcttct
     2101 ggccgatggc gacgtagatg cagtaaagct tctggctctc atcctcgccg gcattggcgg
     2161 tgcgctggtt gatgaaggta tcgagcgcga cggcggactt gccggtctgg cggtcgccga
     2221 tgatcagctc gcgctggccg cggccgacgg gcaccagcgc gtcgagcgcc ttgaggccgg
     2281 tctgcaccgg ctcgtgcacc gacttccggg gaatgatgcc cggcgccttg gtctcgaccc
     2341 ggcggcgctc ggtcgtctca atcgggccct tgccgtcgat cgggttgccg agaccgtcca
     2401 cgacgcggcc gagcaggccc ttgccaacgg ggacgtcgac gatggtgccg gtgcggctga
     2461 ccgtgtcgcc ttccttgatg gcgctgtcat tgccgaagat cacggcgccg acattgtccg
     2521 cctcgagatt gagggccatg ccctggatgc cgttcgggaa ggcgatcatc tcgccggcct
     2581 ggacattgtc gaggccgtgg atgcgggcga tgccgtcgcc gaccgacagc acggtgccga
     2641 cttcgctgac ctgcgcttcg gtgccgaagc tggcaatctg gtcgcgaatg accttcgaaa
     2701 tttctgcggc gcggatgtcc atgattagcc tttcatcgcc atggcgagat tgttcaattt
     2761 ggtgcggatg gagccgtcga tcatctgcga gccgaccttg acgacgagcc cgcccaagat
     2821 ggtgggatcg accgtgagat caaccgcgac atcgcggccg agacgagatt tcaggttgct
     2881 tttcaacgcc gtgacctgct cgccgtcgag cgcgtgggcg gaggtgactt cggcggtgat
     2941 ctcgccgcgg tgattggcgg ccagcatgtt gaaggcgcgg atcacctgcg gcagctgtcc
     3001 cagccggcgg ttgcgggcga gcacgccgag gaaattggtg gtgagcgggt cgatgcccat
     3061 cgagccggcg gcggcaagga tggcgcgggt cgcctcgtcg cggccgatca gcgggctggc
     3121 ggtcagctcc ttgagctccg gcgactgggc gagcgcggcg cgcaacgcgg cgaggctctc
     3181 ggcgaccgcg tccagacgct tgtgatcgcg ggccaattcg aacaaggcgg tcgcgtagcg
     3241 gcctgccaga cttgcctgta tgccgccgga atgctccacg cgcgctcatg cctccaagtg
     3301 ggttcaccca tgcgaagaga ctggcgcaat gagcgccccc gccgggtcgc gcggccctta
     3361 gcaggggcaa aagagggatg caaggcgagc cgcggcgaca gcggtcggcg acttcccgtc
     3421 agcctgcggg gtcgacttcc ggcgcgttct cgacacacca gcgggcggcg gcattgcgct
     3481 cggcgggcgg ggtttgcagg cggaaattgg tctcgcccgc cgtgaactgc cggacctcct
     3541 cctccgccat cgtctggagc agcagggcgc gccactggcc cgccgcctgc tccatcgccg
     3601 gcgcatcgaa gccggtggcg gcgtccgccg cgccggcggc gatcatcaga tagaggtaac
     3661 aggccgagcc gtttgacgtg gcatccgcag ccggaaagtc gggcgggaag gtgcccccgg
     3721 cgggcgccgc ggcgctcgaa ttggccggag ccgccgccgt gatcggcgct ccgccattgc
     3781 catctccgtc ggccgccgcg cgaccgcagc cgctgagcgc gagcaaggcg gcgaggatga
     3841 cgattcgcat aggatatccg atcctcaatc gccgcagccg gtcagcgcct tgggagcgcc
     3901 cggatagcag acataccaga cgtcggtcgc gcccaccttg aagtcctcgg gaacccactt
     3961 gccgcccttg aagcggagca gcgcatcgat cctgaggccg tcggtgaatt cgagctggtc
     4021 cgccggcagc accgaggccg gatcgatccg cccgccgccg ggccgctgcg gctgcgccag
     4081 gaccagcgcc cagccgtcct tcacctccag ccgttcgacg acgaactcga ccggcccgcg
     4141 catcgctttg accacgccgg ggcgaagcgc gtcgaggatc gcgcgccgct gggcgctgcc
     4201 ctcggcagcc gccgccgtgg cgctcacctc cggcgcgtaa gctgcggcgg cggccaaggg
     4261 cgcaatggca agcaatgcgg ccgcgagaag cggcttcgga aaggaattga tcatgcggct
     4321 gccccctgtt tcgcctccgt ccggaggagg cgcgccgcgt cagggacgct ggaatccgac
     4381 tcgtgtcaag gcgcggcgcc tgtcgatccc gggcgacacc gggctgcgcc gtggactgga
     4441 gttttgatcg caagcggcgg gacgcgcgcg ccaatgcaat cgggcatcga ggctgcgaaa
     4501 ggagcccgcc atgacatatc gcatcgaggg tttgccggag aacccgcttc cggacttgcc
     4561 cgagctgtcc gaggccgcca ccatccaccc ccgcaactcc gcgcacggct gcttggccgc
     4621 gcgggtggag cgacactgat ggcgagcgtc atgatgaggg cagaggccgg aacgatcgac
     4681 gaggactggg cctgggcccg ggccttcgcc cgcgaccgga cggcggacgg gctgttcgtg
     4741 atgggggtgc tgaccaccgg catctactgc cgcccgtcct gcgcggcccg gcacccgcgc
     4801 cgtgagaatg tgcgcttctt cgcttgcggg gcggacgcgg cggcgaccgg gctgcgcgcc
     4861 tgcctgcgct gcaagcccga cgacgtcacc cgcgaggcgg cggcgctgga gcgcgcctat
     4921 cgcctgctcg aagcggcgga ggagccgccc gcgctcgccg ccctcgccgc cgcggccggc
     4981 tattcgccgc atcatttcca ccggctgttc aagcgcgcga ccggcgtcac ccccgcccaa
     5041 tatgcgcgcg ggcgccgggc gggggagatg gcgcgcagcc tggcgaagga ggagcgggtg
     5101 acggatgcga tctacgcggc gggttattcc ggcccgggcc gcttctatgc ggacgccggt
     5161 gcccggctcg gcatgacccc gtccgcctgg cgcgacggcg ggcgcggcga gacgatccgc
     5221 tggacgaccg cccagaccga tctcggcgcg atgctggtag cggcgaccgc caagggcatt
     5281 tgccggctgt cgttcgatga aggcgaggcc gagctgcggc gccgcttccc caatgcgagg
     5341 atcgagcccg gcggcagcga gatggccgag ctcgccgagc gggcggtggc cgcggtgaac
     5401 gcgccggagc ggccgcacga cctgccgctc gacgtccgcg gcaccgcctt ccaggaggcg
     5461 gtgtggcgcg agctctcccg cattccgccc ggcgagagcc tgagctatgc ggcgctggcg
     5521 gcgagggcgg ggaagcccgg cgcggcgcgc gcggcgggct cggcttgcgg tgccaatagc
     5581 gtcgccgtgc tgatcccctg tcaccgcgcg cggcgggggg atggctcgct cggcggcttc
     5641 gcctacggcc tcgacaggaa gacggctttg ctcaagcggg agcgtgggct caagcgggag
     5701 cgtgggctcg agcgggagcg cggctgacac cggtcaggtg ccggcggctt tctccttggc
     5761 cggcgcttcg cccgccttga gcgcgccgtc gatattgccg gtgtcgatct gctcgatcat
     5821 cgcggccgcc agcttgcccg cttcggtggc gtgcgggttg aaggcgatcg gcgccaaagc
     5881 gacgcgcgct tcccgggcct tgcggtcgac caggaactga taggccgcca tgaagcgcag
     5941 gtcgttgtcc tgcggagcca gcgcataggc ctggttgagc cccacgaccg cattggcggt
     6001 gggcttcacg ccttgcgcgc cgaagctgct gtagaagagt atcagcggct cgggatcgtc
     6061 cggatcggcc cggttggccg ccgccagcca gcgccggacc tccttccagg tggccgcgtc
     6121 ggtcgccttt ttgtcctcgg ccaccgccat tttcacgcgg cccttgtaga tgagggcctc
     6181 gatcgatttc gggtccaccg ccagagcgcg atcggcggcg gcctcggcct ccgcataata
     6241 gccggcgtca tattccgtct ccgccagcca ggtctgcacg gcgggatcgt tcggaaaagg
     6301 cgcggccgcg cgacgcgcct ccggcaccag cgccttcgcc tgcttgaggt tgaccccgcg
     6361 gcgcgagacg atgcggacgt ccatcatcgc cgcctcgccg gggctgagcg aacggatcgc
     6421 gatctttccg atcatcgaat cgtcggcatt gacccgggtg taggggaggc gctgccgttt
     6481 gacgtagccg tcgagctcgc ccgacagctt gccgaggtct ccgaaggcgg cggtcgccgc
     6541 ttcgaccccg cctttgccgc tgttcagcgc ggtcaaatag ttggcgagct ggccctttcg
     6601 gctcggttcg aaggtaagga aatgggtcag tacccagcct cgtccgtaaa gcgcctcccg
     6661 ctggtgcgag ttcatcctcg acggcaattg ccccatcatc ttgtcgagcg gcagcggatc
     6721 gcccgcgaac agcgaataag cgcgatgctc ggccggcacg ccgaggccga ccgagccgtc
     6781 ttcctcgaac ctggcggtcg cgttgaactc ggcgaagccc tcgctgaacc aggcgggata
     6841 ggcggccgcg tggttctggt agaggaagtg gtgggcatat tcgtggagga ggacggtctc
     6901 ggcgcgcagg aaagaggcat cgctactcag ccggcgcggg atgaaggcga ccgaccctcc
     6961 ggcgcgcggg atgtagaagc cgatgatgaa attgtcgttg ccgggtgcca gccgctggac
     7021 cgccttcaga ttgccgaccg catagacggt cagcctgctg gccggtccca tatctgtgtc
     7081 gggaacgccg tacatcaacc gcatcgcggc gtcgtagcgt tcgagccggg tggcgaattt
     7141 ccggagcgcc gcctcgctat cctcggaata gatgacgaag tgatcgctgc tcgcctcgcg
     7201 ccaggctgcg cccgcgggga cggccgcgaa cagggcgatc atggccagca ggagccgaca
     7261 gagcatcgct tcctccctca actttcgtcg cggcgacggc atgatattcc acagtatttt
     7321 cagatgttca acaagcctcc ccgcggatgt cgccccgggt gcggagccag ggccgtccgg
     7381 aacgttttct gccaccggtc attggcggaa aaagggagag atcggatgga cgacaaggac
     7441 cgcaggccgg cgaccgaccc gccggcgcgg ggcagcaaga tagtctggat catcggcgcg
     7501 atcctgctgc tgctcgtact gttcctgggc atacgcggct gcgactatgc cagccgcgag
     7561 accaattatc aggaaagcgc caacggaacg gtggatgctc ccgacgtcta gcggcgcgcg
     7621 cccggctaga ggaagctgta gggatcgaca tccaccgcga cgcggacgcc gcgcggccag
     7681 tcgagcgcgc ccagccagtc gcggatcacc tcctgcacgt cgagcgatcg cgccgcgtgg
     7741 acgagcaggc gctggcggtg gcggccgcgc agcatcgcca gcggggccgg ggccgggccg
     7801 aacaccgcca tattctctac cttgggggcg gtgcggccga tcagccgcgc ggtctcggtc
     7861 gcttcgccga gatcctcggc cgagacgatg atgcccgcga ggcgcccgaa gggcggcatc
     7921 gccgcctccc gccgggcctg cgtctcggcc gcataaaatc cttcggcatc gccgccgacc
     7981 agcgcgctga tcaccggcgc gccgggctca tgggtctgga ccaggacccg ccccggcttt
     8041 tcgccccgcc ctgcgcggcc cgacacctgg ctgatctgct ggaagctgcg ctcggccgcg
     8101 cgcaggtcgc cgcccgacag cccgagatcg gcatcgacca cgccaaccag ggtcaggttg
     8161 gggaagtgat agcccttggt gaccagttgg gtgccgatga cgatgtcgat cgcgcccgct
     8221 tccatccggc cgacgaactc cgccgccttg gccggcgacc agatcgtatc ggacgtgacg
     8281 atcgcggtcc gcgcctcggg gaacagggcc gcgacctcgt cggcgatccg ctccacgccg
     8341 ggcccgaccg gcaccaggct gtcctcctcc ttgcattcgg ggcaggcgcg gggcggcggc
     8401 atcatgtggc cgcaatggtg gcaggcgagc cggtggacga gccggtgctc gaccatccag
     8461 gcggtgcaat tcgggcattg gaagcggtgg ccgcaggccc ggcagagcgt cagcggcgca
     8521 tagccgcgcc ggttgaggaa aagcagcgac tgctcgcccc gctccagatt ggtctcgagc
     8581 tcgcggacca gcgtcggcgc gagccagcgg ccgcgcggcg gcgggtcctg gatgaggtcg
     8641 atcgcggcga tgtcgggcat ttcggcggcg ccgtagcggt cgggaagctt cacttcggca
     8701 tagacgccga tctcggccat ctgccgggtc tcgatggcgg gcgtcgctga agcgaggatc
     8761 accggcacct tttcgagctt ggcgcgcatc accgcgacgt cgcgggcatg atattggacg
     8821 ccttcctcct gcttgaagct ggtctcgtgc gcctcgtcga cgacgatcag cccgaggttc
     8881 ggataaggga ggaacaaggc cgagcgcgcg ccgaccacca cccgcgcctc gccggtcgcg
     8941 atcgagcgcc aggcgcggcg gcgctgcgat tggcgaaggc cggaatgcca ggccaccggc
     9001 tcgcagccga agcggagcgc gaagcgcttc aggaacggct cggtaagcgc gatctcgggc
     9061 aacagcacca gcgtctggcg gcccatacgg atattctcgg cgaccgcctc gaaatagact
     9121 tcggtcttgc ccgatccggt gacgccatcg agcaggaaag gcgcgaactc ggccgcctga
     9181 acggcgtcca cgaacgcgcg tgccgccgaa cgttgggccg gctcgagccg gggcggatga
     9241 taatcgggat cggggcgggg gaaggggtcg tcgaccgaga ccgtgagcgc ctcgatcgcg
     9301 cccgatttga tcagcccccg gatgacgccg tcggaaacgt cggcggccag cgcgagctcg
     9361 cgcaccaggc cctgccgccc ggcgattcgc tccagcgcct gctcccgctg cggcgtcagc
     9421 cgctccggga ccattccggt ggcgcgatat tcggtcacgg tccgggcgcc gtcgagggcg
     9481 ctcgacgacg gcaaggccat ccggagcacg gacgcgaccg gcgccagata ataatcggcg
     9541 gtccactcga tcagccggcg gagcggcgct ccgatcggcg gcgcgtcgta gatatggagg
     9601 aggggacgga gccgcgcttc ctcgatctcc gccgtctcca gccgatccgg ctcccacg
//
LOCUS       DASVRK010000207         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_414221, whole genome shotgun sequence.
ACCESSION   DASVRK010000207 DASVRK010000000
VERSION     DASVRK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414221"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF589_08165"
     CDS             <1..232
                     /locus_tag="VF589_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257595.1"
                     /translation="MPLYYFNIHCDEFETTDLVGESCATFAQVRGEAIRAARDLVLQD
                     LLSGRVPHQGWIEVEDEDHRAVLSLPLSAAAS"
     gene            complement(325..1299)
                     /locus_tag="VF589_08170"
     CDS             complement(325..1299)
                     /locus_tag="VF589_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8257596.1"
                     /translation="MARVALLSNPRSTGNRSLLPRVRSFCAEHKDIFHYEVEHVEQIG
                     QALKTIARVKPNVLVINGGDGTVQAALTELYHGGHFGDRRPPVAVLPNGKTNLIALDL
                     GAEGDPIAALERVLELARGDMAPHIVERELIQLSDGSVGARPVLGMFLGGAGLADIIL
                     FCRHKIYPLGLPNGISHVLTFLAVVVSILFGVRAGFLPPRPTPVKVSVLKHGELQGQF
                     ALLMVTTLQRLLLNREMPGTGRTLGAMQMMAIERSPSTLVRVLVAAVRGRLGRSSVSG
                     VHVERGDEIRIEGDRSSVILDGELFEASIDRPIVLKPTLPVPFLRLAA"
     gene            complement(1302..1808)
                     /locus_tag="VF589_08175"
     CDS             complement(1302..1808)
                     /locus_tag="VF589_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX8257597.1"
                     /translation="MLKWISLAATGSLALVTPAAAAGAVLGPDAAACRAGSNVPAVLV
                     DVRGFKNRSGKLRVQIYGGNPSEFLAKGKKLKRIDLPVTATRMPVCVALPKAGRYAVA
                     VRHDADANGKSSRQDGGGFSRNPKISLVSMKPDYGQVAIAVGNGVKPVPVVLNYLQGL
                     SIKPIASN"
     gene            2130..3338
                     /gene="lptF"
                     /locus_tag="VF589_08180"
     CDS             2130..3338
                     /gene="lptF"
                     /locus_tag="VF589_08180"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX8257598.1"
                     /translation="MGRLTDRYLARLITVPLIATLVLSAMLLLLEKMLRLFDFVVSEG
                     GPVNVVWRMLANMIPEYLSLGIPIGLMLGILLAFRRLALTSELDSLRAVGLGYGRLLR
                     VPYLFAIALMALNVAIVGFVQPKARYAYEELRFELRSGALGASIKVGEFNNLGKRMTL
                     RIDRSDDEGRHLHGVFVRAEAADGRTLAVTADQGTFLATDQPDTILLRLSNGRLVHDA
                     PNYDSPRVLSFASHDLPIDLPAIETFRGRGDGNEELTIPELVRIGGNEAVDERLRDST
                     RANFHFRMVEIAMMLLLPLVAVALAVPPKRSTSGLGIFVSIVMVVTYHKINQYAEQMA
                     AQDRLDPVLALWTPFAIFAALIVWMYWTLAHRPGGQPIGALERVFAKFGKGVRALLGV
                     TRRRRAVEGG"
     gene            3335..4432
                     /gene="lptG"
                     /locus_tag="VF589_08185"
     CDS             3335..4432
                     /gene="lptG"
                     /locus_tag="VF589_08185"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX8257599.1"
                     /translation="MINVQFFPSKRIALYMAKTFLLRSLAVLAALVIILQTLDLLGES
                     GRILRQAGNGDAQLWYYVSLRLPQLIERFLPFSALLGALITLATLNQNSEIVSMKAAG
                     ISAHQIIAPLVLASILVAAAAFAFQERVVTRATATLNAWQAVEYGPVPTGSRVSSNVW
                     VRRGDDLILARQVNGRGSGTRLRGVTIYARTGETLSALITAPRARPAEGGWLLEEVER
                     FDVARTRTERLPTLLVARGVEPDQFALSNVKANEQNFATLRASIEDLKAAGIPTGALE
                     SGLWHKLSGPLSAVLMPLLAAVAAFGLARTGQLFLRAVAGMALGFAYFVIDNFALAMG
                     NLGAYPPMLAAWAPFALFLLIGETVLIRTEE"
     gene            4429..>5197
                     /locus_tag="VF589_08190"
     CDS             4429..>5197
                     /locus_tag="VF589_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX8257600.1"
                     /translation="MSPSPPDGEAPLTPAPLTPAEVTRGDVARGAGLAGLARAGALIE
                     ALAQPLFVWLFGLATYGLYVVLWGAINLIENVVDLALTAALQRVVPAAEAEAEAHAAV
                     KLALLVTVIPAAFVALAIALNAEAVAALFSAAPGDRASLPQAIRLFAWALPLWTFIEV
                     ATSAARARRAFGPEIRLRIVWEQAARILFALGFFALGFDSLGLILAHLGSLALTALLC
                     VPLLARYYDLGLLARAPVRPALARSLIGSGLALLPSNL"
BASE COUNT          732 a         1973 c         1621 g          871 t
ORIGIN      
        1 catgccgctt tattatttca atatccattg cgacgaattc gagaccaccg acctggtcgg
       61 cgaaagctgc gccacgttcg cgcaggtccg cggcgaggcg atccgcgccg cccgcgacct
      121 cgtcctccag gacctgctca gcggccgcgt gccccaccag ggctggatcg aggtggagga
      181 cgaagaccat cgcgccgtcc taagcttgcc gctgagcgcc gccgcctcct gagcgccgcc
      241 gtcacatgat attgaagact gccgagttcc gcgccgtgag gctgacgccg atcccgcagt
      301 gacggcgggt ccggcagggc tcgatcaggc ggccagcctc aggaacggca ccggcagggt
      361 cggcttgagc acgatcggcc ggtcgatgct ggcctcgaac aattcgccgt cgaggatcac
      421 gctcgaacgg tcgccctcga tcctgatctc gtcgccgcgc tcgacatgga cgcccgacac
      481 gctcgagcgg ccgagccggc cgcgcaccgc agccaccagc accctgacca aggtcgacgg
      541 gctgcgctcg atcgccatca tctgcatcgc gcccaaggtc cgccctgtgc ccggcatctc
      601 gcggttgagc agcagccgct gcagcgtcgt caccatcaac agcgcgaact ggccctggag
      661 ctcgccatgc ttcagcaccg acactttgac cggcgtcggc cgcggcggca ggaagccggc
      721 ccgcaccccg aacaggatcg agacgaccac cgccaggaaa gtcagcacgt ggctgatccc
      781 gttggggagg ccgagcggat agattttgtg gcggcagaac aggatgatgt cggccagccc
      841 ggcgccgccg aggaacatgc cgagcaccgg ccgcgcgccg acgctgccgt cggaaagctg
      901 gatcaactcg cgctcgacga tgtggggcgc catgtcgccg cgggccagct ccagcacccg
      961 ctccagcgcg gcgatcgggt cgccctcggc gccgagatcg agcgcgatga ggttggtctt
     1021 gccgttgggc agcaccgcca ccggcggccg ccggtcgccg aaatggccgc catggtagag
     1081 ctcggtcagc gccgcctgca cggtgccgtc gccgccgttg atcacgagca cgttgggctt
     1141 cacccgcgcg atcgtcttca gcgcctggcc gatctgctcg acatgctcca cctcatagtg
     1201 gaaaatgtcc ttatgctcgg cgcaaaagct cctcacccgg ggcagcagcg accggttgcc
     1261 ggtcgagcgc ggattggaaa ggagcgcgac ccgggccatg ctcagttgct ggcgatcggc
     1321 ttgatcgaca gcccctggag gtagttgagc acgaccggca cgggcttgac gccgttgcca
     1381 accgcgatcg cgacctgtcc gtaatcgggc ttcatgctga ccaacgaaat tttggggttg
     1441 cgcgagaagc cgccgccatc ctggcgcgac gacttgccgt tggcgtcggc gtcgtggcgg
     1501 acggcgaccg cgtagcggcc ggccttcggc aaagcgacgc agactggcat gcgggtggcg
     1561 gtgaccggca ggtcgatccg cttcagcttc ttgcccttgg cgaggaactc ggacgggttg
     1621 ccgccgtaaa tctgcacccg caatttgccg ctgcgattct tgaagccgcg cacgtcgacc
     1681 agcacggccg gcacattcga tccggcgcgg caggcggcgg cgtccggccc gagcacggcc
     1741 ccggccgcgg cggcgggggt caccagcgcc agcgagcccg tcgccgccaa tgaaatccat
     1801 ttcaacatcg tcgatcgctc ccgacagcta cggccagctt cccgcaggac gcggccccta
     1861 tgtccaaaag cccccatcgc cggccaagat gggggagggt tgcggccaaa ttgtggtgac
     1921 gcggcgtcga aaaggtgagc gccgcccgct tcgcggcggg atgcggcgac ccgcagtggc
     1981 cgccgcgctt gatcccttcg ggacggcgct tctcctttga tccccccgct cccgccgcta
     2041 taggctcccc ggcagccggg ccgctccgtc cggaccattc cgcctgggga cggccccggc
     2101 gaacgaggac gaggcacggc aagttgatca tcggcaggct gaccgaccgc tatctggcga
     2161 ggctgatcac ggtgccgctg atcgccaccc tcgtcctgtc ggcgatgctg ctgctgctcg
     2221 aaaagatgct gcggctgttc gatttcgtcg tttcggaagg cgggccggtc aacgtcgtct
     2281 ggcggatgct cgccaacatg atccccgaat atctctcgct cggcattccg atcgggctga
     2341 tgctcggcat cctgctcgct ttccgccggc tcgctctgac ctcggagctg gacagcctgc
     2401 gcgcggtcgg gctcggctat ggccggctgc tgcgcgtgcc ctacctgttc gccatcgcgc
     2461 tgatggcgct caacgtcgcc atcgtcggct tcgtccagcc caaggcccgc tacgcttatg
     2521 aggagctgcg cttcgaattg cgctcgggcg cgctcggcgc ctccatcaag gtcggcgaat
     2581 tcaacaatct cggcaagcgc atgacgctcc gcatcgaccg cagcgatgac gagggccgcc
     2641 acctccacgg cgtcttcgtc cgcgccgagg ccgccgacgg ccgcacgctc gccgtcaccg
     2701 ccgaccaggg caccttcctc gccaccgacc agcccgacac catcctgctc cgcctgtcca
     2761 acggccgtct cgtccacgac gcgccaaatt acgattcgcc gcgcgtgctc agcttcgcca
     2821 gccacgatct gccgatcgac ctgcccgcga tcgagacctt ccgcggccgc ggcgacggca
     2881 atgaggagct caccatcccg gaattggtgc ggatcggcgg caacgaggcg gtcgacgagc
     2941 ggctgcgcga cagcacccgc gccaatttcc atttccgcat ggtcgagatc gcgatgatgc
     3001 tgctgctgcc gctggtcgcg gtcgcgctcg ccgtgccgcc caagcgctcg acctcggggc
     3061 tcggcatctt cgtctcgatc gtgatggtgg tgacctacca caagatcaac caatatgccg
     3121 agcagatggc ggcgcaggac cggctcgatc cggtgctcgc tttgtggacc ccgttcgcga
     3181 tcttcgccgc cctcatcgtg tggatgtact ggacgctcgc ccacaggccc ggcggccagc
     3241 cgatcggcgc gctcgagcgc gtcttcgcca agttcggcaa gggcgtccgc gccctgctcg
     3301 gagtcacccg ccgccgccgc gcggtcgagg gcggatgatc aacgtccagt tcttcccgtc
     3361 gaagcgtatc gccctctaca tggcgaagac cttcctgctg cgcagcctcg ccgtgctcgc
     3421 cgccctcgtc atcatcctcc aaacgctcga cctgctcggc gaatccggcc gcatcctgcg
     3481 ccaggccggc aatggcgatg cccagctctg gtattatgtc tcgctgcggc tgccgcagct
     3541 gatcgagcgc ttcctgccct tctcggcttt gctcggcgcg ctgatcacgc tcgccacatt
     3601 gaatcagaat agcgagatcg tcagcatgaa ggcggccggc atctcggccc accagatcat
     3661 cgcgcctttg gtgctggcca gcatcctcgt cgccgccgcc gccttcgcct ttcaggagcg
     3721 ggtggtgact cgcgcgacgg cgacgctcaa cgcctggcag gcggtcgaat acgggccggt
     3781 gccgaccggc tcgcgagtct cgtccaacgt ctgggtgcgg cgcggcgacg accttatcct
     3841 cgcccgccag gtcaacggcc gcggctccgg cacccggctt cgcggcgtca ccatctacgc
     3901 ccgcaccggc gagacgttga gcgccctcat caccgccccg cgcgcgcggc cggccgaggg
     3961 cggctggctg ctcgaggagg tcgagcgctt cgacgtcgcc cgcacccgca ccgagcggct
     4021 gccgaccctg ctggtcgcgc gcggcgtcga gcccgaccag ttcgcgctct ccaacgtcaa
     4081 ggccaacgag cagaatttcg ccaccctgcg cgcctcgatc gaggatctga aagccgccgg
     4141 catcccgacc ggagcgctcg aatcgggcct gtggcacaag ctttccggcc cgctttcggc
     4201 ggtgctgatg ccgctgctcg ccgccgtcgc cgccttcggc ctcgcccgca ccggccaatt
     4261 gttcctgcgc gccgtcgccg ggatggcgct cggcttcgcc tatttcgtga tcgacaattt
     4321 cgcgctcgcg atgggcaatc tcggcgccta cccgccgatg ctcgccgcct gggcgccgtt
     4381 cgcattgttc ctcctcatcg gcgagacggt gctcatccgg accgaggagt gagcccttcc
     4441 ccgccggacg gcgaggcccc cctcaccccc gcccccctca ccccagccga agtcactagg
     4501 ggcgacgtcg cccgcggcgc cggccttgcc ggcctcgccc gcgccggcgc gctgatcgag
     4561 gcgctcgctc agcctttgtt cgtctggctg ttcggcctcg ccacctacgg cctctatgtc
     4621 gtcctgtggg gcgccatcaa cctcatcgag aatgtcgtcg acctcgccct caccgccgcc
     4681 ctccagcgcg tcgttcccgc cgccgaggcc gaggccgagg cccatgccgc ggtcaagctc
     4741 gccctcctgg tcacggtgat ccccgccgcc ttcgtcgcgc tcgccatcgc gctcaacgcc
     4801 gaggccgtcg ccgctttatt ctccgccgcg cccggggatc gcgcctcgct gccgcaggcg
     4861 atccgcctgt tcgcctgggc cctgccctta tggaccttca tcgaggtcgc gacctccgcc
     4921 gcccgcgccc gccgcgcctt cggccccgag atccgcctcc gcatcgtctg ggagcaggcc
     4981 gcccgcatcc tgttcgcgct cggcttcttc gccctgggct tcgacagcct cggcctgatc
     5041 ctcgcccatc tcggctcgct cgcgctcacc gccttgctct gcgtgccgct cctcgcccgc
     5101 tattacgacc tcggcctcct cgcccgcgcc cccgtccgcc ccgccctcgc ccgcagcctg
     5161 atcggctccg gcctcgccct cctcccctcc aacctcg
//
LOCUS       DASVRK010000208         7387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_39761, whole genome shotgun sequence.
ACCESSION   DASVRK010000208 DASVRK010000000
VERSION     DASVRK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39761"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF589_08195"
     CDS             <1..187
                     /locus_tag="VF589_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257601.1"
                     /translation="HAGAAAADANDRIIYNAANGSLYYDADGSGAAAQILFARVTAGT
                     SLSYADFVAYSDPPAGV"
     gene            331..948
                     /locus_tag="VF589_08200"
     CDS             331..948
                     /locus_tag="VF589_08200"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8257602.1"
                     /translation="MLILSRPAAAALLLAIAPIVSVPAQAESAAAESSIEARAEQLKP
                     GQYVWNPELAPEGPIELVISLPMQMAYVYRSGILIGASTISSGREGYETPLGRFTILQ
                     KSKVHRSNRYNSAPMPYMQRLNWYGVALHGGEIPGYAASHGCVRLPMKFAQKLFGATT
                     LGASVFVANEAPSSPEAALELARSAGPVPAGRGRMKEDMVADGAP"
     gene            1065..1268
                     /locus_tag="VF589_08205"
     CDS             1065..1268
                     /locus_tag="VF589_08205"
                     /inference="COORDINATES: protein motif:HMM:NF041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HEX8257603.1"
                     /translation="MKGLAAAALLAGLAAPAAAQQTGNDTGIATETQERRSEGQTNEF
                     PWDLMGLLGLAGLLGLRRRDDHR"
     gene            1317..1541
                     /locus_tag="VF589_08210"
     CDS             1317..1541
                     /locus_tag="VF589_08210"
                     /inference="COORDINATES: protein motif:HMM:NF041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HEX8257604.1"
                     /translation="MKTTRFSAIAAAAALAALAPAAAPAQGGAVDTSYGNPQPQEERG
                     FPWGLLGLLGLAGLIPRKRGHDHVDNRTRP"
     gene            1609..3228
                     /locus_tag="VF589_08215"
     CDS             1609..3228
                     /locus_tag="VF589_08215"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEX8257605.1"
                     /translation="MRDFSLLLDGLVYTRSRNAKLKLIGDYLKRTPDPDRGYALAALT
                     GELNLPAVKAAAIRGITEERVDPTLFYMSWDYVGDLAETVALLWPKGIAQPDDLDDGT
                     LRISSVVERLWSLGRAEAPGALAAMLDHLDASGRYALLKLATGELRIGVSARLAKTAF
                     AEAYGLDLDAVEEVWHGLSQPYAELFAWAEGKGEQPSARDLPVFRPFMLAHSLDEARV
                     SLDDYAAEWKWDGIRVQAVRAGGETRLYSRAGDDITRSFPDVAAAFDVPGVVDGELLV
                     KGGQQGADAHGGAAASFNALQQRLGRKIVSAKMLGDYPAFVRLYDILFDGDEDLRPLP
                     WSERRARLEAFVPRLDPDRFDLSALIAADSFEALEEIRAGARDAAIEGVMLKRRDSPY
                     VAGRRAGLWYKWKRDPLTADCVLMYAARGSGKRSSYYSDYTFGCWSAAPEEGGGELLP
                     VGKAYFGFTDEELMWLDRFVRNHTLQRFGPVREVEKSLVLEVAFDSIHPSKRHKSGLA
                     MRFPRISRIRTDKPAAEADTIANLQALVPKD"
     gene            complement(3232..4233)
                     /locus_tag="VF589_08220"
     CDS             complement(3232..4233)
                     /locus_tag="VF589_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8257606.1"
                     /translation="MSGGNSRRPRLGFLGTGWIGRHRMEAMLAGGEVEAAAIADPSPE
                     MATEAAKLAPEAQVVGTLDDLLALGLDGVVIATPSALHAEQSIQALEAGAAVFCQKPL
                     GRTAAETRAVVEAARAADRLLAVDLSYRFTEGMARIRELLRAGELGKVFAADLVFHNA
                     YGPDKAWFYDPALSGGGCVMDLGVHLVDLALWALDFPAVTGVAASLAAEGEPLGGRRD
                     KVEDYAVATLELEGGATVRLACSWRLQAGRDAIVEASFYGTGGGAALRNENGSFYDFT
                     AELYRGTGCETLASPPDAWGGRAAADWAARLASGQRFDAEAERLVEVADVLDRIYGR"
     gene            complement(4230..5228)
                     /locus_tag="VF589_08225"
     CDS             complement(4230..5228)
                     /locus_tag="VF589_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8257607.1"
                     /translation="MRGEGAAGKTPAAATMRAAVLAGPGQISIEEVARPEPGPGQVRL
                     RLEGCGVCASNLTPWEGPEWMQFPGEPGGLGHEGWGVIDAVGEGVEGLRPGDRVAALS
                     YKSYAEYDVAEAAAVVPLPDALAGRPFPGEPLGCAMNIFRRSDIQPGQTVAIVGIGFL
                     GAILTRLATDAGARVIAVSRRPFSLDIARRFGAAETIPMDDHNAIIERVKALTGGRFC
                     ERVIEAVGKQWPLDLAAELTAERGKLIVAGYHQDGPRQVNMWLWNWRGIDVINAHERD
                     PKAYAEGIREAVAAVASGRLDPSPLYTHSYPLDRLGEALDATRDRPDGFLKALVTP"
     gene            complement(5312..6154)
                     /gene="fghA"
                     /locus_tag="VF589_08230"
     CDS             complement(5312..6154)
                     /gene="fghA"
                     /locus_tag="VF589_08230"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX8257608.1"
                     /translation="MTLETHSTAKSFGGTQGVYSHFSRETATRMTFSVYLPPQAEAGA
                     KLPVVWYLSGLTCTHANVTDKGGFQRVCAELGLIFVAPDTSPRGDRVPDDEGYDFGHG
                     AGFYVDATEEPFAANYRMYSYVMGELPELVAANFPADMGAQAITGHSMGGHGALTLAL
                     SNPGRFRSVSAFAPIVAPGQVPWGQKAFNGYLGEDRSGWRGHDAVALIEDGARLPDLL
                     VDQGSADPFLVKELRPELLARACEQAGIPLTLRLQEGYDHSYYFISSFMEDHLRWHAE
                     RLRA"
     gene            complement(6228..7340)
                     /locus_tag="VF589_08235"
     CDS             complement(6228..7340)
                     /locus_tag="VF589_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX8257609.1"
                     /translation="MKTKAAIAVAPNRPLEIHEVDLDGPRAGEVLVEILATGVCHTDA
                     YTLEGKDSEGIFPSILGHEGAGIVREVGAGVTSVAVGDHVIPLYTPECRQCKSCLSQK
                     TNLCTSIRATQGKGLMPDGTSRFRLGSETIHHYMGCSTFSNFTVMPEIAVAKVRPDAP
                     FDKICYIGCGVTTGVGAVIWTAGVEPGANVVVFGLGGIGLNVIQGAKLVGANRIVGID
                     VNPAKRAMAQAFGMTDFINPAEIGMDEVVPAILDLTDGGADYSFDCTGNTDVMRQALE
                     CCHRGWGTSIIIGVAEAGREIATRPFQLVTGRVWKGSAFGGARGRTDVPRIVDWYMDG
                     KINIDDLITHTMPLDQINSAFDLMHAGESIRSVVVY"
BASE COUNT         1129 a         2510 c         2588 g         1160 t
ORIGIN      
        1 ccacgccggc gcggcggccg ccgacgcgaa cgaccggatc atctacaatg ccgccaatgg
       61 cagcctctat tacgatgccg acggctccgg cgcggcggcc cagatcttgt tcgcccgggt
      121 cactgccgga acgtccttga gctacgccga tttcgtggcc tattccgacc cgcccgcggg
      181 cgtctagcaa ttgccggcga attcggaggc gggcatggca ccgatccgcc tcgggcgcgt
      241 tatcggggca ggtgaagaag atcggccgat ctcgcggaca gtatcgccag tatcggtcag
      301 cttccttcga gtaacggaag agaagtcggt atgcttattc tatcgcgtcc tgcggcggcg
      361 gcgttgctgc tggccattgc tcccatcgtt tcagtcccgg cacaggcgga gagcgccgct
      421 gcggagagct cgatcgaagc ccgcgcggag cagctcaagc ccggccaata tgtgtggaat
      481 ccggagctcg cgcccgaagg cccgatcgaa ctggtgatca gcctgccgat gcagatggcc
      541 tatgtctatc gctcgggcat tttgatcggc gcatctacca tatcgagcgg ccgggagggg
      601 tatgagaccc cgctcggccg tttcacgatc ctccagaaga gcaaggtcca tcgctcgaac
      661 cgctacaata gtgcgccgat gccgtacatg cagcgtttga actggtacgg tgtcgccctc
      721 cacggcggcg agattcccgg ctatgccgcc tcgcacggct gcgtccggct gccgatgaag
      781 ttcgcgcaga agctgttcgg cgccaccacg ctcggcgcct ccgtgttcgt cgccaacgag
      841 gctccgtcct ccccggaagc ggcgctggaa ctggcacggt ccgcggggcc ggtgcccgcc
      901 gggcggggcc gcatgaagga ggacatggtg gccgacggcg cgccctgacg cgcagccagg
      961 cgaactgatc cagatcaatc acttagaccg gaacgcaagc gttccatacc ggttaggctc
     1021 gtatcttcac ctcatccgaa caggagggta atatgcgagc aagaatgaaa ggcttggccg
     1081 ccgcggcgtt gctggccggc cttgcggctc cggccgcggc gcagcagacc ggcaacgaca
     1141 ccggcatcgc caccgaaaca caggaacgcc gctccgaagg ccagactaac gaatttccgt
     1201 gggacctgat gggcctgctc ggcctcgccg gcctgctcgg cctcaggcgg cgggacgacc
     1261 atcgctaagc cgcccgccca tgcgcaactg atagctcccg caacaggaga gaagccatga
     1321 agaccacccg cttttccgcc atcgccgcag ccgccgcttt ggccgctttg gcgcccgccg
     1381 ccgctcccgc gcagggcggt gccgttgaca cctcctacgg caacccgcag ccgcaggagg
     1441 agcgcggctt cccctggggc ctgctcggcc tcctcggcct cgccggcctg atcccgcgca
     1501 agcgcggcca cgaccatgtg gacaatcgca cccgcccctg accggctgcc ctgacccgca
     1561 ccccccgcgc ccccggagcc aagcgcgcac gggggagttg cactggcgat gcgcgacttc
     1621 tccttattgc tcgacgggct cgtctacacg cgctcgcgca atgccaagct taagctgatc
     1681 ggcgattact tgaagcgcac gcctgatccg gaccgcggct atgcgctggc ggcgctgacc
     1741 ggcgagctca acctgccggc ggtcaaggcg gccgcgatcc gcggcatcac cgaggagcgg
     1801 gtcgatccga ccctgttcta catgagctgg gactatgtcg gcgacctcgc cgagaccgtc
     1861 gccctgctgt ggccgaaggg catcgcgcaa ccggacgacc tcgacgacgg gacgctgcgc
     1921 atctccagcg tggtcgagcg gctgtggagc ctcggccggg cggaggcgcc gggcgcgctc
     1981 gccgccatgc tcgaccatct cgacgccagc ggccgctacg ctttgctgaa gctcgccacc
     2041 ggcgagctca ggatcggcgt ctcggcgcgg ctcgccaaga ccgccttcgc cgaggcctat
     2101 ggcctcgatc tcgatgccgt cgaggaggtc tggcacggcc tcagccaacc ttatgccgag
     2161 ctgttcgctt gggccgaggg caaaggcgag cagccgagcg cgcgcgacct gccggtgttc
     2221 cggccgttca tgctcgccca ttcgctcgac gaggcgcggg tgtcgctgga cgattatgcg
     2281 gccgagtgga aatgggacgg catccgcgtg caggcggtgc gggcgggcgg cgagacccgg
     2341 ctctacagcc gcgccggcga cgacatcacg cgcagcttcc ccgacgtggc ggcggcgttc
     2401 gatgtgccgg gcgtggtcga cggcgagctg ctggtcaaag gcgggcagca gggcgccgac
     2461 gcgcatggcg gcgccgcggc cagcttcaac gcgctccagc agaggctcgg gcgcaagatc
     2521 gtctcggcga agatgctggg cgactatcca gccttcgtgc ggctttacga catcctgttc
     2581 gacggcgacg aggatctgcg gccgctgccc tggtcggagc ggcgggctcg gctcgaggcg
     2641 ttcgtgccgc ggctcgatcc cgatcggttc gatctctcgg cgctgatcgc ggccgacagc
     2701 ttcgaggcgc tggaggagat acgcgccggc gcgcgcgacg cggcgatcga gggcgtcatg
     2761 ctgaagcggc gcgacagtcc ctatgtcgcc gggcgcaggg cggggctctg gtacaaatgg
     2821 aagcgcgacc cgctcaccgc cgactgcgtg ctgatgtatg cggcccgcgg cagcggcaag
     2881 cgctcctctt attattcgga ctatacgttc ggctgctgga gcgcggcgcc cgaggagggc
     2941 ggcggcgagc tgctcccggt cggcaaggcc tatttcggct tcaccgacga ggagctgatg
     3001 tggctcgacc ggttcgttcg caaccacacg ttgcagcgct tcgggccggt gcgcgaggtc
     3061 gagaagagcc tggtgctcga agtcgcgttc gattcgatcc acccgagcaa gcgccacaaa
     3121 tcgggcctcg ccatgcgctt cccccggatc tcgcggatcc gcaccgacaa gccggcggcg
     3181 gaagccgaca cgatcgccaa cctccaggcg ctggtgccga aggactgagg cctagcgccc
     3241 gtaaatgcgg tcgagcacgt cggcgacctc gaccaggcgt tcggcctccg catcgaaacg
     3301 ctggcccgag gcgaggcggg cggcccagtc ggcggcggcg cggccgcccc aggcgtcggg
     3361 cggggaggcg agcgtctcgc agcccgtgcc gcggtaaagc tcggcggtga aatcgtagaa
     3421 ggagccgttt tcgttgcgga gcgccgcgcc gccgccggtg ccgtagaagc tcgcctcgac
     3481 gatcgcgtcg cggccggcct ggagccgcca ggagcaggcg agccgcaccg tcgcgccgcc
     3541 ctcaagctcg agcgtcgcca ccgcataatc ctcgaccttg tcgcggcgcc cgcccagcgg
     3601 ctcgccctcg gccgccaggc tcgcggcgac gccggtgacg gcgggaaagt cgagcgccca
     3661 gagcgcgagg tcgaccaggt ggacgccgag gtccatcacg cagccgccgc ccgacaaagc
     3721 cgggtcgtag aaccaggctt tgtcgggccc ataggcgttg tggaagacga ggtcggcggc
     3781 gaacaccttg cccaattcgc ccgcccgcag cagctcgcgg atccgggcca tgccctcggt
     3841 gaagcggtag gagaggtcga cggcgagcag ccggtcggcg gcgcgggcgg cctcgaccac
     3901 ggcgcgggtt tcggcggcgg tgcggccgag cggcttctgg cagaacaccg ccgcgccggc
     3961 ctcgagcgcc tggatcgatt gctcggcgtg gagggcgctg ggcgttgcga taaccacgcc
     4021 gtcgagaccg agcgctagca ggtcgtcgag cgtgccgacg acctgcgcct ccggcgcgag
     4081 cttcgccgcc tcggtcgcca tttcggggga ggggtcggcg atcgcggccg cctcgacttc
     4141 gccgccggcg agcatcgctt ccatgcggtg gcggccgatc cagccggtgc cgaggaagcc
     4201 gagccggggg cggcgggaat ttcccccgct cacggcgtga ccagcgcctt taaaaagccg
     4261 tccgggcggt cgcgggtggc gtcgagcgcc tcgccgagcc ggtcgagcgg atagctgtgg
     4321 gtgtaaagcg gggaggggtc gagccgcccg gaggcgaccg ccgcgaccgc ctcgcgtatg
     4381 ccttcggcat aggctttggg gtcgcgctca tgggcgttga tgacgtcgat cccgcgccag
     4441 ttccacagcc acatattgac ttggcgcggc ccgtcctggt ggtagccggc gacgatcagc
     4501 ttgccgcgct cggcggtgag ctcggcggcg aggtcgagcg gccattgctt gccgaccgcc
     4561 tcgatcaccc gctcgcaaaa gcggccgccg gtcagcgcct tgacccgctc gatgatcgca
     4621 ttgtggtcgt ccatcgggat cgtctcggcg gcgccgaagc ggcgggcgat gtcgagcgag
     4681 aaggggcggc gcgacaccgc gatgacgcgg gcgccggcgt cggtcgcgag ccgggtcagg
     4741 atcgcgccga ggaagccgat gccgacgatc gcgacggtct ggcccggctg gatgtcgctg
     4801 cggcggaaga tgttcatcgc gcagccgagc ggctcgccgg ggaacggccg cccggcgagc
     4861 gcgtcgggca gcggcacgac ggcggcggct tcggcgacgt catattcggc gtagcttttg
     4921 taggagagcg cggcgacgcg gtcgcccggt cttagccctt cgaccccttc gccgacggcg
     4981 tcgatcacgc cccagccttc atggccgagg ccgccgggct cgccgggaaa ttgcatccac
     5041 tccggccctt cccagggcgt gaggttggac gcgcagacgc cgcagccttc gagccgcagg
     5101 cggacctggc ccgggcccgg ctcgggccgc gccacctcct cgatgctgat ctggcccggg
     5161 ccggccagca cggccgcccg catggtcgcg gcggcgggcg ttttgcccgc ggcgccttcc
     5221 ccccgcatct cagtcaaacc cgatcctttc agctccggaa tgtcgttgcc tttacgatcc
     5281 acgcctccaa aggtttcttc gccccgaggg gtcaggcgcg gagccgttcg gcgtgccagc
     5341 gcaggtgatc ctccatgaag ctggagataa aataatagct gtggtcgtag ccctcctgca
     5401 ggcgcagggt gagcgggatg ccggcttgtt cacaggcgcg ggcgagcagc tcgggccgca
     5461 gctccttgac gaggaacggg tcggccgagc cctggtcgac cagcaagtcg gggagccgcg
     5521 cgccgtcctc gatcaaagcg accgcgtcgt ggccgcgcca gcccgagcgg tcctcgccga
     5581 gatagccgtt gaacgccttc tggccccacg gcacctggcc cggcgcgacg atcggcgcga
     5641 acgccgagac gctgcggaag cggccggggt tgctcagcgc cagcgtgagc gcgccatggc
     5701 cgcccatcga atggccggtg atcgcctgcg cgcccatgtc ggccgggaag ttggccgcga
     5761 ccagctcggg gagctcgccc atgacatagg aatacatgcg gtaattggcc gcgaacggtt
     5821 cctcggtcgc atcgacgtag aagccggcgc cgtggccgaa atcatagcct tcgtcgtcgg
     5881 gcacgcggtc gccgcgcggg ctggtgtcgg gcgcgacgaa gatcagcccg agctcggcgc
     5941 agacccgctg gaagccgcct ttgtcggtga cgttggcgtg ggtgcaggtg agccccgaca
     6001 gataccagac gaccggcagc ttggcgcctg cctcggcctg cggcggcagg tagacgctga
     6061 acgtcatccg cgttgccgtc tcgcggctga aatggctgta gacgccctgc gtcccgccga
     6121 acgactttgc ggtggaatgg gtttcgagtg tcacaattgc gttccccggc gaaggccggg
     6181 gtccagaagg accccgggtc tgggtcccgg ccttcgccgg gacacgatta gtaaaccacc
     6241 acgctcctga tgctctcgcc cgcatgcatc aaatcgaacg ccgagttgat ctggtcgagc
     6301 ggcatggtgt gggtgatgag gtcgtcgatg ttgatcttgc cgtccatata ccagtcgacg
     6361 atccgcggga cgtcggtgcg gccgcgggcg ccgccgaagg cggagccttt ccagacgcgg
     6421 ccggtgacca gctggaacgg gcgggtggcg atctcgcggc cggcctcggc gacgccgatg
     6481 atgatgcttg tcccccagcc gcggtggcag cattcgagcg cctggcgcat cacgtcggta
     6541 ttgccggtgc agtcgaagct gtagtcggcg cccccgtcgg tgagatcgag gatcgcgggc
     6601 acgacctcgt ccatcccgat ttcggcgggg ttgatgaaat cggtcattcc gaaggcctgg
     6661 gccatggcgc gcttggccgg attgacgtcg atcccgacga tccggttggc cccgaccagc
     6721 ttggcgccct ggatgacgtt gaggccgatc ccgccgagcc cgaagacgac gacgttagcg
     6781 cccggctcga cgcccgccgt ccagatcacc gcgccgacgc cggtcgtcac tccgcagcct
     6841 atgtagcaga tcttgtcgaa cggagcgtcc gggcggacct tcgccaccgc gatctcgggc
     6901 atcacggtga agttcgagaa ggtcgagcag cccatataat ggtggatggt ctcggagccg
     6961 aggcggaatc ggctggtgcc gtccggcatc aggcccttgc cctgcgtcgc gcggatcgag
     7021 gtacacaggt ttgtcttctg gctgaggcac gacttacact gccggcattc tggcgtgtag
     7081 agcgggatga cgtggtcgcc gaccgcgacg gaggtcacgc ccgcaccgac ttcccggacg
     7141 attcccgcgc cctcatggcc gaggatggag gggaagatgc cttccgaatc cttgccctcc
     7201 agcgtgtagg cgtcggtgtg gcagactccc gtggcgagga tctcgaccag cacttcgccc
     7261 gcccgcgggc cgtccagatc gacctcgtgg atctccagcg gacggttcgg cgcgacggcg
     7321 atggcagctt tggtcttcac ttgtctctcc tgaagcggcg gtcagcaaga atggccttgg
     7381 cggcatt
//
LOCUS       DASVRK010000209         9197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_231364, whole genome shotgun sequence.
ACCESSION   DASVRK010000209 DASVRK010000000
VERSION     DASVRK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231364"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..589
                     /locus_tag="VF589_08240"
     CDS             161..589
                     /locus_tag="VF589_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8257610.1"
                     /translation="MLLRKRERLIRRLIVVEDEPLVAFDNERLLEDAGYEVVATVDNL
                     ADAARAIAEEELHLVLTDMTLNGDGDGLDVARAAAGRSIPVLFVSGNFPAGGEGLCIG
                     WLVKPYNDKMLRSALDAIEATLQGRPVKKLPNGLSLYEQA"
     gene            668..2047
                     /locus_tag="VF589_08245"
     CDS             668..2047
                     /locus_tag="VF589_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8257611.1"
                     /translation="MTELASTAPAERRGFVATMGPYLRKRPLAALLLGVSSGFPLALL
                     LGTMTFWLTKVGIDKKTIGFAVGLTTPYTLKFLWAPFIDRVKLPLLTGLFGQRRAWLF
                     FIMALLFVSVWMLGASDPGRSLGTFAFWAIVTAFLGATQDIVIDAYRIEILPDEELPH
                     GTANNQFGYRLGAFAAGVGTIAMASDEGFGLGWATAYGLTAFCVVPGIIAAFWAGRGL
                     HDKVLAKEPPMSAGKWLETTIVGPFREFLRRRGAILILLFVLIYKLGDAMGQIMLNPM
                     IVELGFSDTEFLVINKGVGFWGLIVGIALAAPFMAWLGMGRSLFASGVLMMLSNLMFA
                     ILASQGHSNLWLTIAVATEQVTSGIGLTVFVTYLSGLSNLAYTATQFALLSSFAAVGR
                     TWLSSPAGIAAEEFGWVGFWIMTMAVAIPGLVLLWLLWRRGFVVETVRQPKSEADETV
                     SEVPKPAAA"
     gene            complement(2182..2559)
                     /locus_tag="VF589_08250"
     CDS             complement(2182..2559)
                     /locus_tag="VF589_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8257612.1"
                     /translation="MDLNQVTLVAHDYERSVAFYRLLGLRLIVDAPPRYARFECGNGA
                     TFSIHVEPGRPPASGIVYFETEDLDARAAALEAAGLAFLSAPRDQSWLWREARLLDPA
                     GNEICLYHAGPNRRFPPWRVEGS"
     gene            2908..5769
                     /locus_tag="VF589_08255"
     CDS             2908..5769
                     /locus_tag="VF589_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8257613.1"
                     /translation="MRQWKAIRATGISIGAMAGLLAAHSAANAQASTVPEQPLESGAT
                     AGSEPNEEEAIVVTGSRIRRDPLSLDAPVTFVDREDIERTGLVSTAEILQRLPSSGGA
                     LNSRFNNSGNFGNPPDGGGVGAGAAEVDLRYLGSKRVLVLIDGLRFVNGASASGVPGS
                     TDLNAIPESMIERIEVLQDGASAIYGSDAIAGVVNIITKKRQDGLLASAQLGIYDEGD
                     GFTQNYQLSYGLGSEESGTNIVVGGNYVKQGAVSSADRDISQFPTPGATSCLEGGCSS
                     GTPLGRFIVLGQDLTLRGPVIGRVPVYDPENPTGGDFKAFGDADRFNFAPFNFIQTPV
                     ERYGAFVNLEQRITENVDLSVKAIYNHRNSQNQAAPLPLFVGPDAGNGNLLDTIVIDE
                     TNPFNPFGVTLDTSNYAFIGRRFVENGPRRYNQKVETYYVTGTLDGSFDIGGRDWYWD
                     VNGLWGRNKAKQTVLGNVNAANLARALGPVADCTGACVPFNIFGGAGSITQSMIDYVA
                     FTQRDSSEQELWDVSANLSGSLFELPGGPLGVAIGVEHRDQEGRFDPDAIVAAGLGSD
                     IPALPTRGGFNVDEVYAELNAPLLADRPFFDLLEVSVAARLSDYSTSGSTTTFKAGVN
                     WKPIEDLRLRASWAEGFRAPSIGELFGTPSRFDQEIIDPCSAVNGAIPAAVRENCIAA
                     GVPANGSYVQANPQLPVVTGGNEALAPETSESWVFGAVYSPSWAPRLSLEANYFDITV
                     DGAIQSIDAEVLLNRCASTADPLSCAAIDRTGSGAISQIRGLLQNIASIETDGVDFTF
                     NYKTPETSAGTFGLFWSNTFLFNYTVTVPSTDGVTVIDREGTEQGSPDQAFPKYKSTA
                     ILDWDYDFFGASVTGRYISGVEEEDGNKLGRRLYIDAQVRFDVETNGRDDGFGFAVGV
                     NNLFGTDPPACFTCGLNNMDPTTYDVPGRFVYARASYRM"
     gene            complement(6068..6469)
                     /locus_tag="VF589_08260"
     CDS             complement(6068..6469)
                     /locus_tag="VF589_08260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX8257614.1"
                     /translation="MSIDRRTFVAAAGLVAAAPVAALAEPGKKPPLYGLIGKMTAQPG
                     RRDQLIAILVEGVSAMPGCLSYIVAEDPADPNLIWIAEAWESKEAHAASLSLPSVRAA
                     IAKGRPLIAGMAAVAETAPVGGHGLALPAGR"
     gene            complement(6520..>9197)
                     /locus_tag="VF589_08265"
     CDS             complement(6520..>9197)
                     /locus_tag="VF589_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257615.1"
                     /translation="RNLLEGGDGEDELNIRTVAGAESTILTSLGGRGVFKGSSDTGPP
                     FLDFAGIETISLYLLSGPDTLQIDNAAITGLEFLYVGLGAHENDFTPDGEIDTIILRG
                     GAARDTVVLSEASGAVDVRGMGPDRIVVDYLEGMDTLTLSGGGGSDSIDASAGNTPSK
                     LILIGGGGNDRLLGSAAPDELFGEDGNDIVTGGAGGDLVILGAGDDQAFWNAGDGDDA
                     LNAGGGNDQLILAGSIDDFWEIGLAAGGFKVSGLYGSVRASETEIVSFRGTHSNTYSI
                     GTLVGSGLNRLNFTPDAEMAVFELTGTSGNDHFDLDVIDDALVVLGLGAELWIDGGPI
                     AVRVHGGDGNDVMDTRGAAASSFVFFGGIGDDIFFASESVDQVLGDSGQDTVDYSGSV
                     AGVTVTLGGVDAVGQGFGGSADQDVYSDIENASGSAFDDLLSGNRFKNVLAGAGGDDQ
                     LYGREEDDRLVGGAGSDRTDGGIGNDVHVIDRASDVVIERVNEGTDRILAQASYALAA
                     DAHVEILSTASNAGLAAINLTGNGFANTLIGNAGANRLNGGRGIDTLSGLGGNDLYVV
                     DHADDRVVEAAGGGADRVFASVSYTLAAPAQVETLSTLSSAATTAIDLTGNGFANRLV
                     GNAGANLLNGGGGADSLAGLGGDDRYVVDSQSDAVAERAGDGDDRIYASASYALAAGS
                     HVEILSTINSAAAAAINLAGNALANSLIGNAGDNLLHGGAGNDSLAGLGAADALFGGA
                     GTDRLVGGAGADNFHFDTTLNEAANVDLIADFTTDDAIHLNRGIFAGIGADGTLAPSA
                     FRAGTAATDASDRIIHDSATGNLYYDADGEDGAAQILFGRVAPGIVLAAGDFVAYSSP
                     ANLAPAESGGFDAAMARSFPLLLEERLAPPDCQFA"
BASE COUNT         1492 a         3025 c         3140 g         1540 t
ORIGIN      
        1 ttggggggct tgggcaaggc gggctccgac cggtagagga gggctgtact cggacagccg
       61 gaaaattatt cgcgcccaag cgactggtcg ccccggcacc gcttgcctcc atccttcgtt
      121 acgcagtcaa accgattatc cggaggcggg attccagtag atgttgttgc gtaagcgcga
      181 aaggctaatc cggcggctca tcgtggtcga ggacgagccg ctggtggcgt tcgacaatga
      241 acggctgctc gaggatgccg gctatgaggt ggtcgcgacg gtcgacaatc tcgccgacgc
      301 ggcgcgggcg atcgccgagg aagagctcca cctcgtgctg accgacatga cgctcaacgg
      361 cgacggcgac gggctcgacg tcgcccgcgc ggcggccggg cggagcatcc cggtgctgtt
      421 cgtctccggc aatttcccgg ccgggggcga gggcctgtgc atcggttggc tggtcaagcc
      481 ctataacgac aagatgctgc gctcggcgct cgacgcgatc gaggcgacgc tgcaggggcg
      541 gccggtgaag aagctgccga acggcctcag cctttacgag caggcttagg ccggggcgtt
      601 ccggttgggt ggcatggccc ccatcaaagg gactatcgtt ccggttcccg cacgctagaa
      661 aagcggaatg acagaattag ccagtaccgc gcccgccgag cggcgcggat tcgtcgccac
      721 catgggcccc tatcttcgca agcgcccgct cgcggctttg ctgctcggcg tttcgagcgg
      781 cttcccgctt gccttgctgc tcggcacgat gaccttctgg ttgaccaagg tcggcatcga
      841 caagaagacg atcggcttcg cggtcggcct caccacgccc tatacgctga aattcctgtg
      901 ggcgccgttc atcgaccggg tgaagctgcc gctcctcacc ggcctgttcg gccagcggcg
      961 ggcgtggctg ttcttcatca tggccctgct gttcgtctcg gtatggatgc tgggcgcgag
     1021 cgatccgggc cgaagcctcg gcaccttcgc cttctgggcg atcgtcaccg ccttcctcgg
     1081 cgccacccag gacatcgtca tcgacgccta tcgaatcgag atcctgcccg acgaggaatt
     1141 gccgcacggc accgccaaca accagttcgg ctaccggctc ggcgccttcg ccgccggcgt
     1201 cggcacgatc gcgatggcgt cggacgaggg tttcggcctc ggctgggcga ccgcctacgg
     1261 cctcaccgcc ttctgcgtcg ttcccggcat catcgccgcc ttctgggcgg gccgcggcct
     1321 ccacgacaag gtgctcgcca aggagccgcc gatgtcggcc ggcaagtggc tcgagacgac
     1381 catcgtcggg ccgttccggg agttcctgcg aaggcggggc gcgatcctga tcctgctgtt
     1441 cgtgctgatc tacaagctcg gcgacgcgat gggccagatc atgctgaacc cgatgatcgt
     1501 cgaattgggc ttcagcgaca ccgagtttct ggtgatcaac aagggggttg gcttctgggg
     1561 gctgatcgtc ggcatcgcgc tggcggcccc gttcatggct tggctcggca tgggccgatc
     1621 gctgttcgca tcgggcgtgc tgatgatgct cagcaacctc atgttcgcga tcctcgcctc
     1681 gcagggccat tccaatctgt ggctgacgat cgcggtggcg accgagcagg tgaccagcgg
     1741 catcggcctt accgtgttcg tgacctattt gtcgggactg tcgaaccttg cctacaccgc
     1801 cacccagttc gccttgctgt cgtccttcgc cgccgtcggg cggacctggc tgtcgtcgcc
     1861 ggccggcatc gccgcggagg agttcggctg ggtcggcttc tggatcatga ccatggcggt
     1921 cgcgatccca gggctggtgc tgctctggct gctgtggcgg cgcggcttcg tcgtcgagac
     1981 ggtgcgccag ccgaagagcg aggcggacga gacggtgtcg gaggtgccca agccggctgc
     2041 cgcttagggg gtgctcggcg ggttgcgccg tcattcgagc ggaagctggt acgtcattcc
     2101 agcggaagct ggaatctccc ttcttctgcc ccggccgcag gattccagct tgcgctggaa
     2161 tgacggggag gtgctgacgg ctcaggatcc ttcgacgcgc cagggcggga agcggcggtt
     2221 ggggccggca tgatagaggc agatttcgtt gccggcgggg tcgagcaggc gggcttcccg
     2281 ccacagccag gattggtcgc ggggggcgga gaggaaggcg aggccggcgg cttcgagggc
     2341 ggcggcgcgg gcgtcgagat cttcggtctc gaaatagacg atgccacttg cgggcgggcg
     2401 gccgggctcg acatggattg agaaggtggc gccattgccg cactcgaagc gggcgtaacg
     2461 cggcggggcg tcgacgatca gccgcaggcc gagcaggcgg tagaaagcga ccgagcgctc
     2521 ataatcgtgc gcgacgagcg tcacctggtt caagtccatg aggctttgtc gacgccccgg
     2581 acgcgactgg caagcggcgg cgcggcttcg tcattccagt gaacgctgga atctcctccc
     2641 cgtcattcca gcgccccccg tcattccagc gcaagctgga atgttgcggc ccgggacaga
     2701 agaagggaga ttccagcttt cgctggaatg acggttaggc tgcaatcacg cgtcgcgagg
     2761 tgtaacgcgg gcgcaacgat cgtggggaaa atgacacggc ttgcggctcc ggagccacat
     2821 tccgcttcct cgcgcggcgg ccgcgtagtt taacccaggt tggcggcacg cgaatggacg
     2881 cgggcgcgaa acaaagcggg gcgagacatg cggcagtgga aggcgatccg ggcgacgggc
     2941 atctcgatcg gcgcgatggc ggggctgctc gccgcgcatt cggccgccaa cgcgcaggcc
     3001 tcgaccgtgc ccgaacagcc gctcgaaagc ggcgccaccg ccggctccga gcccaatgag
     3061 gaggaggcga tcgtcgtcac cggatcgcgc atccgccgcg atcccttgtc gctcgacgca
     3121 ccggtgacct tcgtcgaccg cgaggatatc gagcgcaccg gcctcgtctc gaccgccgaa
     3181 atattgcagc ggctgccgag ctccggcggc gcgctcaaca gccggttcaa caatagcggc
     3241 aatttcggca acccgcccga cggcggcggc gtcggcgccg gcgcggccga ggtcgacctg
     3301 cgctatctcg gctcgaagcg ggtgctggtg ctgatcgacg ggctgcgctt cgtcaacggc
     3361 gcctcggcga gcggcgtgcc gggctcgacc gacctcaacg ccatccctga aagcatgatc
     3421 gagcggatcg aagtgctgca ggacggcgcc tcggcgatct acggatcgga cgcgatcgcc
     3481 ggcgtggtca acatcatcac caagaagcgc caggacgggc tgctcgcctc ggcccagctc
     3541 ggcatttacg acgagggcga cgggttcacc cagaattacc agctgagcta cgggctcggc
     3601 agcgaggaaa gcggcaccaa catcgtcgtc ggcggcaatt atgtgaagca gggagcggtg
     3661 agctcggccg accgcgacat ctcgcaattc ccgacgccgg gcgcgacttc ctgcctcgag
     3721 ggcgggtgca gctcgggaac gccgctcggc cggttcatcg tgctcggcca agacctgacc
     3781 ctgcgcgggc cggtgatcgg gcgggtgccg gtgtacgacc ccgaaaaccc gaccggcggc
     3841 gacttcaagg cgttcggcga tgccgaccgc ttcaacttcg cgccgttcaa cttcatccag
     3901 accccggtcg agcgctacgg cgccttcgtc aatctcgagc agcgcatcac cgagaatgtc
     3961 gacctgtcgg tgaaggcgat ctacaatcac cgcaactcgc agaaccaggc ggcgccgctg
     4021 ccgctcttcg tcgggccgga cgccggcaac ggcaatctgc tcgacacgat cgtgatcgac
     4081 gagaccaacc cgttcaaccc gttcggggtg acgctcgata ccagcaatta cgccttcatc
     4141 ggccgccgct tcgtcgagaa cgggccgcgc cgctacaacc agaaggtcga gacttattat
     4201 gtgaccggca cgctcgacgg ctcgttcgat atcggcgggc gcgactggta ttgggacgtg
     4261 aacggcctgt gggggcgcaa caaggccaag cagaccgtgc tcggcaacgt caacgccgcc
     4321 aacctcgccc gcgcgctcgg cccggtggcg gactgcaccg gcgcgtgcgt gccgttcaac
     4381 atcttcggcg gcgccggctc gatcacccag tcgatgatcg attacgtcgc cttcacccag
     4441 cgcgacagca gcgagcagga attgtgggac gtttcggcga acctcagcgg cagcctgttc
     4501 gagcttccgg gcgggccgct cggagtcgcg atcggcgtcg agcatcgcga ccaggagggg
     4561 cggttcgacc ccgacgcgat cgtcgcggcc gggctcggct ccgacattcc ggcgctgccg
     4621 acgcgcggcg gcttcaacgt cgatgaggtc tatgcggagc tgaacgcgcc gctgctcgcc
     4681 gaccggccgt tcttcgacct gctcgaagtg tcggtcgcgg cgcggctgtc cgattattcg
     4741 acctcgggct cgaccaccac cttcaaggcg ggggtaaact ggaagccgat cgaggacctg
     4801 cgcctcaggg cgagctgggc cgagggcttc cgcgcgccga gcatcggcga attgttcgga
     4861 acgccgtcgc gcttcgacca ggagattatc gacccctgct cggcggtgaa cggcgcgatt
     4921 ccggccgcgg tgcgcgaaaa ttgcattgcc gcgggcgtcc ccgccaacgg ctcctacgtc
     4981 caggccaacc cgcagctgcc ggtggtgacc ggcggcaacg aggcgctggc gccggaaacg
     5041 tcggaaagct gggtgttcgg cgcggtctac agcccgtcct gggcgccgcg gctgtcgctc
     5101 gaggccaatt atttcgacat cacggtcgac ggcgcgatcc agtcgatcga cgccgaagtg
     5161 ctgctcaacc gctgcgcctc gaccgccgac ccgctgagct gcgcggcgat cgaccgcacc
     5221 ggatcgggcg cgatctcgca gatccgcggc ctcctccaga atatcgccag catcgagacg
     5281 gacggcgtcg acttcacgtt caactacaag acgccggaga ccagcgcggg cacgttcggc
     5341 ctgttctgga gcaacacctt cctgttcaac tatacggtga cggtgccgtc gaccgacggc
     5401 gtcaccgtca tcgaccgcga agggaccgag cagggcagtc cggaccaggc cttcccgaag
     5461 tacaagtcga ccgcaatcct cgactgggat tacgactttt tcggcgcttc ggtgaccggc
     5521 cgctatatca gcggcgtcga ggaggaggac ggtaacaagc tcgggcggcg gctctatatc
     5581 gacgcgcagg tgcggttcga cgtcgagacc aacgggcgcg acgacggctt cggcttcgcg
     5641 gtcggcgtca acaacctgtt cggcaccgat ccgccggcct gcttcacctg cggcctcaat
     5701 aatatggacc cgaccaccta cgacgtgccg ggccggttcg tctacgcgcg ggccagttac
     5761 cgcatgtagg cgcggcgcga acggcatgtg cgggcgggca ttgtgcccct gcgaaggcag
     5821 gggcccagag ccgggttgcc gcttcagcgg cagcccggtt ttcgcagcgg ctggggaagc
     5881 ttggcgccgt aggacgtacc gtcccggact cgcgcggtgt ctacgagctt ggttggcggc
     5941 ccctgccttc gcaggggcac caagaacggc aaaccccaag cctgcgccac gtgccgccaa
     6001 catcgccaga agcgacaccc gcaggcttcg cgagatcgcc tgacactgct cggctggaaa
     6061 gcggacgtca gcggccggcc ggcaacgcga ggccgtgccc gccgaccggg gcggtttcgg
     6121 ccacggccgc catgccggcg atcaggggcc ggcccttggc gatcgccgct ctgaccgaag
     6181 gcagcgaaag cgaggccgca tgggcctcct tgctttccca tgcctccgct atccagatca
     6241 ggttcgggtc cgccggatcc tcggcgacga tatagctgag gcatccgggc atcgccgaga
     6301 ccccctccac caggatcgcg atcagttggt cgcggcggcc gggctgtgcg gtcatcttgc
     6361 cgatcaagcc gtaaagaggc ggcttcttgc cgggctcggc cagggccgcg acgggggcgg
     6421 cggcaaccaa tccggccgct gccacgaatg ttctgcgatc gatgctcatc ggcgtggact
     6481 agccgccggg aagcgccgca acaactccgg ccgcttcgct caggcgaact ggcaatccgg
     6541 cggcgccagc ctctcctcga gcagcaaggg gaaggaacgg gccatcgccg cgtcgaagcc
     6601 gccgctttcg gcgggggcca ggttggcggg actggaataa gcgacgaaat ccccggccgc
     6661 gaggactatg ccgggagcga cgcgcccgaa caggatctgg gcggcgccgt cctcgccatc
     6721 ggcgtcgtag tagaggttgc cggtcgccga atcatggatg atcctgtcgc tggcgtcggt
     6781 cgccgcggtc ccggcgcgga aggcggaggg ggcgagcgtg ccgtcggcgc cgatgccggc
     6841 gaagataccc cgattgaggt ggatggcgtc gtcggtggtg aagtcggcga tgaggtcgac
     6901 gttggccgct tcgttcaggg tcgtgtcgaa gtggaaattg tccgccccgg cgccgccgac
     6961 caaccggtcg gtcccggcgc cgccgaacag cgcgtccgcg gcgcccagcc cggccagcga
     7021 gtcgttcccg gcgccgccgt gcaataggtt gtcgccggca ttgccgatga ggctgttggc
     7081 aagcgcgttg ccggcgaggt tgatggcggc ggcggcggcg ctgttgatcg tgctcaatat
     7141 ttccacatgg gagccggcgg cgagcgcata gctggcgctg gcgtagatgc ggtcgtcgcc
     7201 gtcgccggcg cgctcggcca ccgcatcgct ttggctgtcg acgacgtagc gatcgtcgcc
     7261 gccgaggccg gcgagcgagt cggcgccgcc gccgccgttc agcagattgg cgccggcatt
     7321 gccgaccaag cggttggcga agccgttgcc ggtgaggtcg atggcggtgg tggcggcgct
     7381 gctgagggtg ctgagggttt cgacctgggc gggcgcggcg agcgtgtagc tgacgctggc
     7441 gaacacccgg tcggcgccgc cgcccgcagc ctcgaccacg cggtcgtcgg catgatcgac
     7501 gacatagagg tcgttgccgc cgaggccgct gagcgtgtcg atgccgcggc cgccgttcaa
     7561 ccggttcgcg ccggcattgc cgatcagggt gttggcgaag ccgttgccgg tgaggtttat
     7621 cgcggcgagg ccggcattgc tggcggtgct caaaatctcg acatgcgcat ccgccgcgag
     7681 cgcgtagctg gcctgcgcga ggatccggtc cgtgccctcg ttcacgcgct cgatcacgac
     7741 gtcgctggcg cgatcgatga cgtggacgtc gttgccgatg ccgccgtcgg tgcgatcgga
     7801 gccggcgccg ccgacgagcc tgtcatcctc ttcccgcccg taaagctggt cgtcgccgcc
     7861 cgcgccggcc aggacgttct tgaaccggtt gccggacagc aagtcatcga acgcggagcc
     7921 gctcgcattc tcgatgtcgc tataaacatc ctggtcggca ctgccgccga acccttggcc
     7981 cacggcgtcg acaccgccca acgtgaccgt aacgccggcc acagaaccgc tatagtccac
     8041 tgtgtcttgg ccgctgtcgc ccaatacctg atcgacgctc tcactagcga aaaagatatc
     8101 gtcgccaatc ccgccgaaga atacgaaaga agatgcagcg gcaccgcgag tgtccatgac
     8161 atcgttgccg tcacctccat ggaccctcac cgcgatcggg ccgccatcga tccacagctc
     8221 cgccccaagc ccgagaacga ccaacgcatc gtcgatcaca tcaagatcga aatgatcatt
     8281 acctgaagtt ccggtcagct cgaacacagc catctcggcg tcgggcgtga agttcagccg
     8341 attgagcccg ctgccgacca aagtaccgat actgtaagtg ttgctatgtg ttcctctgaa
     8401 cgacacgatc tcggtttcac tagctcgaac cgagccgtat aagccgctaa ccttgaagcc
     8461 tccggcggcg aggccgatct cccagaaatc gtcgatactg ccggcgagga taagttgatc
     8521 gtttcccccg ccggcattga gcgcgtcgtc gccgtcgccg gcgttccaga acgcctgatc
     8581 gtcgccggcg ccgaggatga cgaggtcgcc gccggcgccg ccggtgacga tgtcgttgcc
     8641 gtcctcgccg aacaattcgt ccggggcggc cgaaccgagc agccggtcgt tgccgccgcc
     8701 gccgatcagg atgagcttgg acggcgtgtt gccggcgctc gcatcgatcg aatcgctgcc
     8761 gccgcctccg gagagggtga gcgtgtccat gccctccaga tagtccacca cgatccgatc
     8821 cgggcccatg ccgcggacgt ctaccgcgcc cgacgcttcg ctgaggacga cggtgtccct
     8881 ggccgcgccg ccgcgcagga tgatggtgtc gatctcgccg tcgggcgtga agtcgttctc
     8941 gtgcgcgccg aggccgacat agaggaattc cagcccggtt atcgccgcat tgtcgatctg
     9001 gagggtgtcc gggcctgaca gcaggtagag cgagatcgtc tcgatgccgg cgaaatcgag
     9061 aaagggcggg ccggtatcgc tgctgccctt gaagacgccg cggccgccca gtgaggtgag
     9121 gatggtgctc tcggcgccgg cgacggtccg gatgttgagc tcgtcctcgc cgtctccgcc
     9181 ttcgaggaga ttgcggc
//
LOCUS       DASVRK010000210        18361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_479982, whole genome shotgun sequence.
ACCESSION   DASVRK010000210 DASVRK010000000
VERSION     DASVRK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 18361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18361
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479982"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..733)
                     /locus_tag="VF589_08270"
     CDS             complement(137..733)
                     /locus_tag="VF589_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8257616.1"
                     /translation="MDDDRDVRRSISFMLGAAKLSSRPFASGTDFLASLQELEPGCIL
                     LDIRMPEVDGFEVMSELARQDVEWPVIVMTGHGEVSIAVRAMKLGAVDFIEKPFDEGM
                     LLVSLERAFALLEDRREQAARKRHAVEQIGRLTGRERDVLQGLMAGLPNKMLARRFDI
                     SLRTVEMHRANMMDRMQVSSLAEALTLAVRAGLPPLES"
     gene            complement(1024..1893)
                     /gene="ligD"
                     /locus_tag="VF589_08275"
     CDS             complement(1024..1893)
                     /gene="ligD"
                     /locus_tag="VF589_08275"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8257617.1"
                     /translation="MSERIVEGVRLSNPDKILYPDQGITKGELADYYVAVAERMLPHV
                     ALRPVTMVRCPTGQEKKCFYQRHAGSGVLEQLKPVKVPGFEEDYLYIEDLPGLIAMVQ
                     MGTLEIHPWGVTVAEPARPDRIIFDLDPDEGLGFDAVVAAAIEVRERLRALGLESFVK
                     TTGGKGLHVVVPVEPVTEWREAKAWSRTLSERIAADAPDKYLIKISKAERVGRIFIDY
                     LRNDPTSTAVAPYSTRSRPGAPVAVPLFWEELERPLDPAAFTIATVPERIRRTEDPWA
                     EMAASRQRLPDLD"
     gene            complement(1890..2933)
                     /locus_tag="VF589_08280"
     CDS             complement(1890..2933)
                     /locus_tag="VF589_08280"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX8257618.1"
                     /translation="MFDRLSVKPGLAPMEAKSVDRLPGGDGWQYEPKWDGFRCLVFRD
                     GAEVELQSKSGKPLGRYFPEVAAAVAALPADRFVLDGELIITLGGILSFDALQMRLHP
                     AESRIRKLARDTPAQLMLFDCLLGANGESLVEEPLAARRRALEAFHADAGAADLQLSP
                     YTEDQALAQSWLDMAGGALDGVVAKRRDGRYEPGERAMLKIKQLRTADCVVGGFRYAA
                     KGREVGSLLLGLYNKEGKLDHVGFTSAIPAEQKPELTRRLEALVEPPGFTGNAPGGPS
                     RWSNERSGDWQPLRPELVAEVRYDHVTGNRFRHGTKFLRWRPDKAPAQCRMDQLEHEA
                     RPAELAATLEGAA"
     gene            3160..3747
                     /locus_tag="VF589_08285"
     CDS             3160..3747
                     /locus_tag="VF589_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEX8257619.1"
                     /translation="MLQAGAAAGLVTAARPVFATVAPFQPQIDPRLKARALLALEARR
                     HMVRKTDVIGITDFSRPSREARFYIVDVASGRVSSHLVAHGRGSDPLHVGYLQMFSNE
                     SGSEATSRGGYVTGDYYQGKYGYSMRLRGLDRSNSNAEPRAIVIHQAWYAEPQVVADH
                     GKLGRSEGCFAFGASSHSEVLSRLGPGRFLYADKV"
     gene            3810..5294
                     /locus_tag="VF589_08290"
     CDS             3810..5294
                     /locus_tag="VF589_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8257620.1"
                     /translation="MDEAPPPESDYDAALLQPGRNCLRIARAGRASLIVDAADYYRIA
                     REAMLGARSRILLIGWDIDTRVALVEKEEAAGAPNCLGPLLSWLSRRRPKLEIYVLAW
                     DEGVLSLPGRGTTLLRMLEWWMNDHVTFKFDSSHPLDASHHQKILVIDDAIAFCGGID
                     ITADRWDTRGHKDDDPGRRRPFTRRCYEPWHDTIMAVDGDAARALGDLGRLRWKIATD
                     CQLSPVDDACRGDLWPEALDPNFRDVDVAIARTRGKEGNISEVREIEALFVDLIAAAR
                     RFVYFETQYFASRVVTEAIARRLEEPDGPEFVVVNPKTAYGWLDEAVMSPAREALVRA
                     LAERDRHGRFRIYTPVTGRGSDIYVHAKVMIVDDVFLRVGSANLNNRSMGLDSECDVL
                     IDGRPRDDVRATIGKLRCDLLAEHLGLEQADVAACFEETGSLIAAIEQLRGSGRTLVP
                     FEPREPNAAEKKLARSELLDPEGPDELFERRARPGLLSGLRRRR"
     gene            complement(5298..6563)
                     /locus_tag="VF589_08295"
     CDS             complement(5298..6563)
                     /locus_tag="VF589_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8257621.1"
                     /translation="MYHSLSRSALPLLLACAAMPAAAQLETGEAPTKEQVATGEVPAT
                     PFDEAETVYRSFGGTMVAPAWSKPAAEDLLAYVDRSGEDGLDPADYAPDALRAALAGG
                     EQAALDRAATDSFLRVSADLALGNVPMAARDDWHLKDNDLTGAQQFTLMRKAIQDGTV
                     RDTLASLLPTHPQYAELKGVLATTSDKTTADKVRVNLDRWRWLPRDLGRRYVIVNVPA
                     YTVALVEDGHVVARHKAVVGAPKTATPQLGATATGVILNPWWEVPKSITPEVQGKRGY
                     VRVKAGEGWRYRQPPGPANALGRMKVVMPNNYAIYLHDTPSKSAFNKPARAFSHGCIR
                     TQDPLAFAELLLGNPEWDRAAIDAAIASGKTVEAKATTPTPVYITYFTAAAAQTGKIV
                     SYKDVYGRDGSVVVALNQRAGTALASAGE"
     gene            6848..7240
                     /locus_tag="VF589_08300"
     CDS             6848..7240
                     /locus_tag="VF589_08300"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEX8257622.1"
                     /translation="MNDEQSQVAIPLLEERLITGKREVETGRVRVRTVIDEHETIVRD
                     TLARGSVEVERVPMHVEVSEVPQPREEGDVLIVPVVQEVLVVEKKLILTEELRLHRKT
                     STEDYAQPVTLRSQRAVVEREETSGETT"
     gene            7258..8202
                     /locus_tag="VF589_08305"
     CDS             7258..8202
                     /locus_tag="VF589_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX8257623.1"
                     /translation="MSRTVTAMFDSRPEAEAAKARLISSDIDADSIRIIDKSYSGSSG
                     GSESSDGQGFWASLKDMFMPDEDRHAYGEAIERGGFLLCAQVDETDADRAISMLDETD
                     SVDFDQREQQWRSDGWQGQYAGASGFGGGTSSSFQGDANTGFDQSKRSQTVAEEHIPI
                     VEEELRVGKREVSRGGARVRSYIRETPVHEQINLREEHVSVERRPVNETLRAGDLEGN
                     DILRERNIEMTETAEESVVAKEARVREELVVRKTAEEHVENIDDTVRRTEVEVDEGAA
                     GSGDRSAFGSFGNQQTESGGGFSESRSDLERTDRDKGF"
     gene            complement(8261..8692)
                     /locus_tag="VF589_08310"
     CDS             complement(8261..8692)
                     /locus_tag="VF589_08310"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX8257624.1"
                     /translation="MNTRTFALIWGIVFLLAGASGFIPGLWHPAPAHYPDLVVDSFYG
                     DALGLFPVNILHNIVHLLFGVWGLMAHKSLPAAKNYARSVAVIYAVLMVAGFVPGLNT
                     MFGLVPLFGNDPWLHLFLLLPAAYFGFVHRDRADDRTADRV"
     gene            complement(8764..8961)
                     /locus_tag="VF589_08315"
     CDS             complement(8764..8961)
                     /locus_tag="VF589_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257625.1"
                     /translation="MNHQELDWLDQRATTEFQMAQQAPNLHAARPHYAMAVQYLGRAV
                     ELRRRLRSSARKREGVLTEPK"
     gene            complement(9029..9664)
                     /locus_tag="VF589_08320"
     CDS             complement(9029..9664)
                     /locus_tag="VF589_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8257626.1"
                     /translation="MSHIVLLGDSIFDNGVYVPGEPDVVRQLQATLPEGWRATLLAVD
                     GAVTRSVKAQLERLPADATHLVVSVGGNDALGESHVLGAAVRSVGEGVALLAAAQDRF
                     AGDYREMLEAVLARGLPTALCTIYDTPASAPNHRIIKTGISIFNDVITRAAFAAALPL
                     IDLRLICAEDDDYANPIEPSAKGGDKIARAIASLVAGGAGAVSTVVARAPG"
     gene            complement(9676..11202)
                     /locus_tag="VF589_08325"
     CDS             complement(9676..11202)
                     /locus_tag="VF589_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8257627.1"
                     /translation="MNNFTSFDSRAETERLFERLGLQLPREGAIEARSPIDGRTIGRV
                     AEQGAGDVAEAVEQAHQAHLAWRLVPPPRRGELVRLFGQVLREEKETLGRLVSLEAGK
                     ILQEGLGEVQEMIDICDFAVGLSRQLYGLTIATERPGHRMMETWHPLGVVGVISAFNF
                     PVAVWAWNACLAFVCGDGVVWKPSEKTPLCGAAVQALFERAAERFGDAPAHLSMVVQG
                     GRAAGEALVDDRRVALVSATGSTRMGREIAPRVAARFGRSLLELGGNNGMVVAPTADL
                     DLAERAITFAAVGTAGQRCTTLRRLFVHDEVYDELVPRLKQVWAGVPVGNPLADGVLV
                     GPLIDGAGFDAMQAALEQARGEGGRLEGGERLYPEDAPEAWYVRPAIVQMQDQSAVVL
                     RETFAPILYVLRYATLDEAIDYHNAAPQGLSSSIFTNDLREAELFLSAAGSDCGIVNV
                     NIGPSGAEIGGAFGGEKETGGGRESGSDAWRNYMRRSTNTINYSRELPLAQGINFAMP
                     "
     gene            11451..12401
                     /gene="cysD"
                     /locus_tag="VF589_08330"
     CDS             11451..12401
                     /gene="cysD"
                     /locus_tag="VF589_08330"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX8257628.1"
                     /translation="MTQLLQAREFDMNAAAEPSRLTHLQRLEAESIHILREVAAEAEK
                     PVMLYSIGKDSAVMLHLARKAFYPAPPPFPLLHVDTTWKFRAMYEMRERAAQEAGMEL
                     LVHHNPEAKEKGINPFDHGPLHTDMWKTEGLKQALDKYGFDAAFGGARRDEEKSRAKE
                     RVFSFRSANHRWDPKNQRPELWRLYNARKAKGESMRVFPISNWTELDVWQYIHLENIP
                     IVPLYFSAPRPTVERDGLLLMVDDDRFRLREGEQPVERSVRFRTLGCYPLTGAVESEA
                     KTLPDIIREMLLTTTSERQGRAIDHDQAASMEKKKQEGYF"
     gene            12401..14314
                     /gene="cysN"
                     /locus_tag="VF589_08335"
     CDS             12401..14314
                     /gene="cysN"
                     /locus_tag="VF589_08335"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX8257629.1"
                     /translation="MATQVQESSYKADELIAEDIGAYLEQHQHKSLLRFITCGSVDDG
                     KSTLIGRLLYDSKMIFEDQLAALEADSKRHGTQGQEIDFALLVDGLAAEREQGITIDV
                     AYRFFATDKRKFIVADTPGHEQYTRNMVTGASTAELAVILIDARKGVLTQTRRHSFLV
                     NLIGIKNVVLAINKMDLVDYSQARYEEILAEYRAFASEIGIGNFVAIPISGLRGDNIT
                     ERSATMPWYVGPTLLAHLETVQLDEEHLRTSSFRMPVQWVNRPNHEFRGFSGMIAGGT
                     VRKYDAVRVLPSGRTSTVSRIVTLDGDLDSAVAGQSVTITLADEVDCSRGDVIVAADQ
                     PLEVADQFEATIVWMDDEAMLPGRPYWLKIGTTTVSATVNEPKYQVNVNTLEQLAAKT
                     LELNAIGVANLQLDRAIPFTPYAENHDLGGFILVDKLTNATVGAGMIHFALRRSHNIH
                     WQHLEVDRNAHARLKGQKAKVLWFTGLSGAGKSTIANLVEKKLHDMGRHTFLLDGDNV
                     RHGLNKDLGFTDADRVENVRRVGEVARLMADAGLIVMCAFISPFRAERRMVREMMPEG
                     EFAEVWVDTPLAEAERRDVKGLYKKARSGALANFTGIDSPYEEPENAELRIDTTKMSP
                     EEAAEHIVAELLR"
     gene            14311..15036
                     /locus_tag="VF589_08340"
     CDS             14311..15036
                     /locus_tag="VF589_08340"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX8257630.1"
                     /translation="MTDADLAARIAEGAGAILLAVRAAGLIEGKALGAAGDRIANAFI
                     LDALQAQRPDDAVLSEESVDTTDRLEQERVWIVDPLDGTREYSEGREDWAVHIALAIG
                     GRPALGAVALPAQGLVLRSDAPPALPALADGPVRLLVSRTRPPAEAERVAEALGAELV
                     PMGSAGAKAMAVLLGQGDVYLHAGGQYEWDNCAPAAVALGAGLHATRLDGSPLEYNRA
                     DPKLPDLLICHRSLAERVRAALA"
     gene            15042..16109
                     /locus_tag="VF589_08345"
     CDS             15042..16109
                     /locus_tag="VF589_08345"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX8257631.1"
                     /translation="MAGQRIQPVILSGGSGTRLWPLSVPERPKQLLALTGEETMIQLT
                     AERVRDAERYAAPIAVCGERHADQVEAQLTAIGTPPALVIVEPSARNTAPAIALAALA
                     AAPDDCLLVMPSDHVVADVAAFGAVVERALAAAADGWIVTFGIEPDRPETGYGYIFRG
                     EALAEGVNEVERFVEKPDRATAEAYLAAGQCSWNSGIFLFRAGDYLEALERHAPDILA
                     AVRAAVAGGRREAGRLYPEASAFARSPSISIDYAVMEKVERAAVAPVSMGWSDIGSWD
                     ALYDLIEKDAHGNALSGEVVAIGGSGSLIRTTGPKVAAIAVNDLIILATPEAVLVVPR
                     GESQRVKEAVEAIERLGGGAK"
     gene            complement(16154..16993)
                     /locus_tag="VF589_08350"
     CDS             complement(16154..16993)
                     /locus_tag="VF589_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEX8257632.1"
                     /translation="MTAVRLTPKRVEKPWGRRDLAMFGTVPYEAEPVGEIWFEDPAGA
                     ARPLLVKYLFTSEKLSIQVHPGDADARAAGYPSGKDEAWVVLGADAGAVIGIGLRRPV
                     TPDELRAAALDGSIEQLLDWRPAAAGDVYYSPAGTIHALGQGLALIEVQQNVDLTYRL
                     YDYGRPRELHLDESIAVANPVPYEPPFAPRAEGEGRLILAEGPAFVLERWTGLIEGRL
                     SAAAAPLWLIPVAGTGRVAGEPLEPGEVWMVEAETALALDPGADLLIAYAEPKARADL
                     LKS"
     gene            17205..>18361
                     /locus_tag="VF589_08355"
     CDS             17205..>18361
                     /locus_tag="VF589_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8257633.1"
                     /translation="MASSSFLVDRGGSEPLVLPQSAEEQHSFVHAVRALGSSKELLRL
                     KLYSSLMAVDIAGLALAFMLASWIRLGSPLHGEGIDTLAVVLPLFIGIALNSRAYSIE
                     VLKAPRVGIRRALQSLVFALVAVIGIIFYLKASETFSRLLFAIGGASAVGFVALGRWG
                     LGHFAGRRCGWSFTNEVLLVDGLQVFPRDGEIVLFADQVNLSPRTDDPELLDRIGRLL
                     KNCDRVLVACPAERRAAWSAMLKGTDVDVEVLVPELDGLGALDLRRFGDRSTVLVGCG
                     PLGLRDRVAKRGLDLAIALPATLLLLPLMLIVGIAIKLESAGPVFFSQPRVGRGNRIF
                     HVLKFRSMRADSADSAGVRSTARGDDRITRIGAFIRRTSIDELPQLINVVLG"
BASE COUNT         3047 a         6183 c         6212 g         2919 t
ORIGIN      
        1 gtcagccggt cacttgcgcg atcatggtcg cggaggatgg cggcgatgaa atcggcctcg
       61 gcgtcggccg cgacgattgc gaaccgcggc tcggtcgcgg cggctcggcg gtagcggcgc
      121 gaaccctccg ccaatctcag ctttccagcg gcggcagccc ggcccgcacc gccagggtca
      181 aggcttcggc cagactggat acctgcatcc ggtccatcat gttcgcccgg tgcatttcga
      241 ccgtgcgcaa gctgatgtcg aagcgcctcg ccagcatctt gttcggcagg ccggccatca
      301 ggccttgcag cacgtcacgc tcccggccgg tgaggcggcc gatctgttcg accgcatggc
      361 gcttgcgcgc ggcctgctcg cggcgatcct cgagcaaagc gaaggcgcgt tcgaggctga
      421 ccagcagcat gccttcgtcg aatggctttt cgatgaagtc gaccgcgccg agcttcatcg
      481 cgcggacggc gatcgaaact tcgccatgac cggtcatcac gatcaccggc cattccacgt
      541 cctgacgcgc cagctcgctc atgacttcga agccgtccac ttccggcatg cggatgtcga
      601 gcaatatgca gccaggctcg agctcctgga ggctggcgag gaagtcggtt ccgctggcga
      661 aggggcgcga gctaagcttg gcggcgccca gcatgaagct gatcgaacgg cggacgtcgc
      721 gatcatcgtc cacgacatga acatattggg atgacagaac atctatcctt caggacagca
      781 aggcccacgc cgctcgataa tgagcttccg ccgccccaag tcaaccggtc ggaggctgct
      841 cgccggccgc cggccgatgc ccaagaaata aggcggcggg agtggggttc ccgccgcctt
      901 gtggcttgca tcaggaccag ttgatgcacc cctgttagac accatcacgg gtgaaggggg
      961 caaggctcca gcataccgta ttcgccgcat atgcggtttg cacgaggtct ccgccggagg
     1021 ggctcagtcg agatcgggca gccgctggcg cgacgccgcc atctcggccc acgggtcctc
     1081 cgtccgccgg atccgctccg gcacggtcgc gatggtgaag gcggcgggat cgagcggccg
     1141 ctcgagctcc tcccagaaaa gcggcactgc caccggagcg cccggccggg accgggtcga
     1201 gtaaggcgct acggcggtcg aggtcgggtc attgcgcaaa tagtcgatga agatacggcc
     1261 gacccgctcc gccttggaga tcttgatgag atatttgtcg ggtgcatcgg cggcgatccg
     1321 ctccgacaag gtgcgcgacc aggccttggc ctcgcgccat tcggtcaccg gctcgaccgg
     1381 cacgaccacg tggaggccct tgccgccggt cgtcttgacg aagctttcca ggcccagcgc
     1441 ccgaagtcgc tcgcggacct cgatcgcggc ggcgaccact gcgtcgaagc cgaggccttc
     1501 gtccggatcg aggtcgaaga tgatccggtc cggccgcgca ggctcagcga ccgtgacgcc
     1561 ccaggggtgg atttcgagcg tgcccatctg caccatcgcg atcagcccgg gcaggtcctc
     1621 gatgtagaga taatcctctt cgaaccccgg caccttcacc ggcttcaact gctcgagcac
     1681 gcccgagccg gcgtggcgct ggtagaagca tttcttctct tggcccgtcg ggcagcgcac
     1741 catcgtcacc ggccgcaagg cgacgtgcgg cagcatccgc tcggccacgg cgacataata
     1801 atcggcgagc tcgcccttgg tgatgccctg atcggggtag aggatcttgt cggggttgct
     1861 gagccggacc ccctcgacga tgcgctcgct catgccgccc cctccaatgt cgccgccagc
     1921 tcggccgggc gcgcctcatg ttcgagctgg tccattcggc actgggccgg ggccttgtcc
     1981 gggcgccagc ggaggaactt ggtgccgtgg cggaagcggt tgccggtgac atggtcgtaa
     2041 cggacttccg ccaccagctc cggccggagc ggctgccagt cgccggagcg ctcgttgctc
     2101 caccggctcg ggccgccggg ggcgttgccg gtgaagcccg gcggctcgac cagcgcctcg
     2161 agccgccgcg tgagctccgg cttttgctcg gccggaatgg ccgaggtgaa gccgacatgg
     2221 tcgagcttgc cttctttatt gtagagcccc agcagcagcg atccgacctc cctgcccttg
     2281 gcggcgtagc ggaagccgcc gaccacgcag tcggcggtgc ggagctgctt gatcttcaac
     2341 atcgcgcgct cgcccggctc gtagcggccg tcgcggcgct tggcgacgac gccgtcgagc
     2401 gccccgccgg ccatatccag ccacgattgg gcgagcgcct gatcctcggt ataaggggag
     2461 agctgcaggt cggccgcgcc ggcatcagca tggaaggctt cgagcgcgcg ccgccgcgcc
     2521 gccagcggct cctcgaccag cgattccccg ttggcgccga ggaggcaatc gaacagcatc
     2581 agctgggcgg gcgtatcccg cgccagcttc ctgatccggc tctcggcagg gtggagccgc
     2641 atctgcagcg catcgaacga gaggatgccg ccaagcgtga tgatgagctc gccgtccagc
     2701 acgaaccggt cggccgggag cgccgccacc gccgccgcca cttcgggaaa gtagcggccg
     2761 agcggcttgc ccgatttcga ctggagctcg acctcggccc cgtcgcggaa cacgaggcag
     2821 cgaaagccgt cccattttgg ctcatattgc cagccgtccc cgcccggcag ccggtcgacc
     2881 gatttggcct ccatcggcgc cagtccaggc ttcacgctca agcgatcgaa caaggccatc
     2941 tccttgtgcc atcgccatag acgctccccc gcgtggccgg ttcctttgag gccggggcat
     3001 accggacgtt aagctatgtt ccccattatg cggcgaaacg cgtcagggct gaggcgaacg
     3061 cgagtcgcgg cgccgccagt tcgttgagag agcgagacaa aaactgtaca aaggccgggt
     3121 tatccgggga gggttacgac catcattacg cgtaggaact tgcttcaggc gggagcggcc
     3181 gccggcctcg tcaccgccgc acgcccggtc ttcgcgaccg tggcgccctt ccagccgcag
     3241 atcgatccgc gcctcaaggc gcgcgccctg ctcgcgctcg aggcgcggcg tcacatggtc
     3301 cgcaagacgg acgtgatcgg catcaccgat ttcagccgcc cgtcgcgcga ggcacgcttc
     3361 tatatcgtcg acgtcgccag cggccgggtc agttcgcacc tggtggcgca cggccgcggc
     3421 tccgatcccc tgcatgtcgg ctacctccag atgttctcga acgagagcgg gtcggaggcg
     3481 acgtcgcggg gcggctacgt caccggtgat tattaccagg gcaagtacgg ctattcgatg
     3541 cgcctcaggg ggctcgaccg gagcaacagc aacgccgagc cgcgcgccat cgtcattcac
     3601 caggcctggt atgccgagcc gcaggtggtc gccgaccatg gcaagcttgg gcgcagcgag
     3661 ggctgcttcg ccttcggggc ctccagccat tcggaagtgc tctcgcgcct cggcccgggc
     3721 cgcttcctct acgccgacaa ggtctgagct ctaccgagcg cggccgttac gggcgcgaaa
     3781 cccggccgcc gctgcggcgt tccgcatgag tggacgaggc acctccgccc gaatctgatt
     3841 atgatgcggc tttgctccag ccgggccgca attgcctgcg catcgcccgg gcgggccggg
     3901 cgtcgctgat cgtcgatgcc gccgattatt accgcatcgc gcgcgaggcc atgctcggcg
     3961 cccgctcccg tatcctgctg atcgggtggg acatcgacac ccgagtggcc ctggtcgaga
     4021 aggaagaggc ggcgggcgcc cccaattgcc tggggccctt gctgtcctgg ctgtcgcgca
     4081 ggcgaccgaa gctcgaaatc tacgtgcttg cctgggatga gggcgtgctg agcctgcccg
     4141 gccgcggcac cactttgctg cgcatgctgg aatggtggat gaacgatcac gtcaccttca
     4201 agttcgacag ctcgcacccg ctcgacgcta gccaccacca gaagatactc gtgatcgacg
     4261 atgccattgc gttttgcggc ggcatcgaca tcaccgccga ccgctgggac acgcgcggcc
     4321 acaaggacga cgacccgggg cgccgccggc ccttcacccg ccgctgctac gaaccctggc
     4381 acgacaccat catggccgtg gacggcgatg ccgcccgggc gctcggcgat ctcggccggc
     4441 tgcgctggaa gatcgcgacc gattgccagc tgtcgccggt cgacgatgcg tgccgcggcg
     4501 acctctggcc ggaggcgctc gatcccaatt tccgcgacgt cgacgtcgcg atcgcgcgca
     4561 cccgcgggaa ggaaggcaac atcagcgagg tgcgcgagat cgaggccttg ttcgtcgatc
     4621 tgatcgcggc ggcgcggcgg ttcgtctatt tcgagactca atatttcgca tcgcgcgtgg
     4681 tgacggaagc aatcgcgcgc cggctcgagg agccggacgg gcccgaattc gtggtcgtca
     4741 atccgaagac ggcttatggc tggctcgacg aagcggtgat gagcccggcc cgcgaagcgc
     4801 tggtgcgggc gctggccgag cgcgaccggc acggccggtt ccggatctac accccggtga
     4861 ccgggcgggg ctccgacatc tatgtccatg ccaaggtgat gatcgtcgac gacgttttcc
     4921 tgagggtcgg ctcggccaat ctcaacaacc ggtcgatggg cctcgacagc gaatgcgacg
     4981 tgctgatcga cggccgcccg cgcgacgatg tccgcgccac gatcggcaag ctgcgatgcg
     5041 atctcctggc cgagcatctc ggcctcgaac aggccgatgt cgcggcctgc ttcgaggaga
     5101 cggggtcgct gatcgcggcg atcgagcagc tgcgcggcag cgggcggacg ctggtgccgt
     5161 tcgagccgcg cgagccgaat gccgccgaga agaagctcgc caggagcgag ctgctcgatc
     5221 ccgaagggcc ggacgaactg ttcgagcggc gggcgcggcc ggggctgctg tccgggctca
     5281 ggcgacggcg ctagccctca ttcgccggcg gaggccaaag cggtgccggc gcgctggttg
     5341 agcgcgacca cgacgctgcc gtcgcgcccg taaacgtcct tgtagctgac gatcttgccg
     5401 gtctgggccg cggcggcggt gaaataggtg atgtagaccg gcgtcggcgt cgtggccttc
     5461 gcttcgaccg tcttgcccga agcgatcgcc gcgtcgatcg ccgcccggtc ccattccgga
     5521 ttgccgagca gcaattcggc gaaggcgagc ggatcctggg tgcggatgca gccgtggctg
     5581 aacgcgcgcg cgggcttgtt gaacgccgac ttggacggcg tgtcgtggag atagatggcg
     5641 taattgttgg gcatcaccac cttcattcgg ccgagcgcgt tggccggccc cggcggctgc
     5701 cggtagcgcc agccctcacc ggccttgacc cgcacatagc cgcgcttgcc ctgcacttcc
     5761 ggggtgatgc tcttgggcac ttcccaccac gggttgagaa tgacgccggt cgcggtcgcg
     5821 ccgagctgcg gcgtcgccgt cttgggcgcg ccgaccaccg ccttgtggcg ggcgacgacg
     5881 tggccgtcct cgaccagggc cacggtgtaa gcggggacgt tgacgatcac atagcgccgg
     5941 ccaaggtcgc gcggcagcca ccgccagcgg tcgagattga cccgcacctt gtcggcggtc
     6001 gtcttgtcgg aggtcgtggc gagaacgccc ttgagctcgg catattgcgg gtgggtcggc
     6061 agcagcgagg ccagcgtgtc gcgaacggtg ccgtcctgga tcgccttgcg catcagcgtg
     6121 aattgctggg cgccggtgag gtcgttatcc ttgaggtgcc aatcgtcgcg ggccgccatc
     6181 ggcacgttgc cgagcgcgag atccgccgag acgcgcagga agctgtcggt agccgcccgg
     6241 tcgagcgcgg cctgctcgcc gcccgccagc gccgcccgca gcgcgtccgg cgcataatcg
     6301 gcgggatcga gcccatcctc cccgctgcgg tcgacataag cgagcaggtc ctcggccgcg
     6361 ggcttgctcc aggccggcgc caccatcgtg ccgccgaacg agcgatagac cgtctccgct
     6421 tcgtcgaaag gcgtggccgg aacttcgccg gtcgcgacct gctccttggt cggggcctcg
     6481 cccgtttcca gttgcgcggc cgccggcatc gccgcgcagg cgagcaggag cggtaaagcg
     6541 gagcgtgata ggctgtgata catgactgtc cctctaaccc agcctcttgg ccgaagccag
     6601 ctttcgccaa gctgaccgcg acgcggcgag cgatttccgc gccgcctgct tccctggcgc
     6661 aacaccctga ggggcaacat atttgtcaca gccgccggcg cgacaaccgc agaatcggcg
     6721 cccgactcga cttgacctaa attaaggacg gctcggtagc agaccggtgc cggggcgcga
     6781 cgagccgctg gaacgggtct gcccacgacc ggttgaaggg ctttccgaaa cccaaccgac
     6841 aggccgcatg aacgacgagc agagccaggt tgctattcct ttgctggagg agcggctgat
     6901 caccggcaag cgcgaggtcg agaccggccg cgtccgggtc cggaccgtga tcgacgagca
     6961 tgagacgatc gtgcgcgaca cgctcgcccg cggcagcgtc gaggtcgagc gcgtgccgat
     7021 gcatgtcgag gtctcggaag tgccgcagcc gcgtgaggag ggcgacgttc tgatcgtgcc
     7081 ggtcgtgcag gaagtgctgg tggtcgagaa gaagctgatc ctcaccgagg agctgcggct
     7141 gcaccggaag accagcaccg aagactatgc gcaacccgtc acgctgcgtt cgcagcgggc
     7201 ggtggtggag cgcgaagaga cgagcgggga aacaacctaa gcaaggagta agacctaatg
     7261 tcgcgtaccg taaccgccat gttcgacagc cgccccgagg ccgaagccgc caaggcccgc
     7321 ctcatctctt ccgatatcga tgccgacagc atccgcatca tcgacaaaag ctattccggc
     7381 agttccggcg gttcggaatc gtccgacggc cagggtttct gggcctcctt gaaggacatg
     7441 ttcatgcccg acgaggatcg ccacgcttat ggcgaagcga tcgagcgcgg cggtttcctg
     7501 ctctgcgccc aggtcgacga gaccgacgcc gaccgcgcca tctcgatgct cgacgaaacc
     7561 gacagcgtcg atttcgacca gcgcgagcaa caatggcgta gcgacggctg gcagggccaa
     7621 tatgccggcg cgtcgggctt cggcggcggc acctcgtcca gcttccaggg cgacgccaat
     7681 accggtttcg accaaagcaa gcgctcgcag acggtggcgg aagagcatat cccgatcgtc
     7741 gaggaagagc tccgcgtcgg caagcgcgag gtcagccgcg gcggcgcccg cgtccgatcc
     7801 tacatccgcg agactccggt ccacgagcag atcaacctgc gcgaggagca tgtcagcgtc
     7861 gagcggcggc cggtcaacga gacgcttcgc gccggggatc tcgaaggcaa tgacatcctt
     7921 cgcgagcgca acatcgagat gacggaaacg gctgaagagt cggtcgtcgc caaggaagcg
     7981 cgggtccggg aagagctggt cgtgcgcaag accgccgaag agcatgtcga gaatatcgac
     8041 gacaccgtgc gccgcaccga agtcgaagtc gacgagggcg ccgccggatc gggcgaccgt
     8101 tccgcgttcg gcagcttcgg caatcagcag accgagagcg ggggcggctt cagcgaatcc
     8161 cgttccgacc tcgagcggac ggaccgcgac aaaggcttct gatctccgga tcgaagcgaa
     8221 agcgagaggg cggccccagg gccgccctct tttttttgcg tcaaacgcgg tcggccgtgc
     8281 ggtcgtctgc ccggtcgcgg tgaacgaagc cgaaataggc ggccgggagg agaaggaaga
     8341 ggtgcagcca gggatcgttg ccgaacagcg ggaccagacc gaacatcgtg ttcaaccccg
     8401 gtacgaaccc cgcaaccatc agtaccgcgt agatgacggc gacgctgcgc gcataatttt
     8461 tcgcggcggg gaggctctta tgagccatca ggccccagac gccgaacagc aggtggacga
     8521 tgttgtgcag gatgttgacc gggaagaggc cgagcgcatc cccgtagaag gaatccacta
     8581 ccagatcggg ataatgcgcg ggagccggat gccagaggcc gggaatgaag ccggaggcgc
     8641 cggcgagcag aaacacgatg ccccagataa gcgcgaaggt tcgggtattc atcagaagtc
     8701 ctcctgttgg cgttttacgc gcacccggtt cgttttttca ccgtcccccc aagcgcctag
     8761 cgctcatttc ggttccgtca gaaccccctc tcgtttgcgc gcgctgcttc gcagccggcg
     8821 cctcaattcc accgcgcggc cgagatattg gaccgccatc gcataatgcg ggcgagccgc
     8881 gtgcaggttc ggcgcctgct gggccatctg aaattcggtg gtggcccgct ggtcgagcca
     8941 atcgagctcc tgatggttca tgaccctctc cagattatcg ttctggagca acattatgcc
     9001 gaatgttccg gccccgcact aaccttgatc agcccggcgc ccgggccacc actgtcgaca
     9061 ccgcccctgc cccgccggcc accaaactgg cgatcgcgcg ggcgatcttg tctccgccct
     9121 tggctgaagg ctcgatcggg ttggcgtaat cgtcatcctc agcgcagatc aggcgcaggt
     9181 cgatcagcgg caacgcggcc gcgaaggcgg cccgggtgat gacgtcgttg aagattgata
     9241 tgccggtctt gatgatccgg tgattgggcg ccgaagcggg cgtgtcgtag atggtgcaga
     9301 gcgccgtcgg cagccccctc gccagcaccg cctccagcat ctcccgataa tcgccggcga
     9361 agcgatcctg cgcggcggcc agcaaggcca cgccctcacc gactgatcga acagccgcgc
     9421 cgaggacgtg ggattcgccg agcgcatcgt tgccgccgac gctgacgacg aggtgggtgg
     9481 cgtcggccgg caagcgctcg agctgcgcct tcacgctgcg ggtgacggcg ccgtccaccg
     9541 ccagcaaagt cgcgcgccag ccctcaggaa gagtcgcctg caattgtctc accacgtccg
     9601 gctcgccggg cacatagacg ccgttgtcga agatggaatc gccgaggagg acgatgtggc
     9661 tcatcgcccc ccaactcacg gcatcgcgaa gttgatcccc tgggcgagcg gcagttcgcg
     9721 gctgtaattg atcgtgttgg tggagcggcg catgtagttg cgccaggcat cggagccgct
     9781 ttcgcggccg ccgcccgtct ccttctcgcc tccgaacgcg ccgccgatct cggcgcctga
     9841 cggaccgata ttgacgttga cgatgccgca gtcggagccg gcggcggaga ggaacagctc
     9901 cgcctcgcgc agatcgttgg tgaagatcga ggagctgagc ccctgcggcg ccgcattatg
     9961 atagtcgatc gcctcgtcca gcgtcgcata gcggagcacg tagaggatcg gcgcgaaagt
    10021 ttccctgagc acgacggcgc tctggtcctg catctggacg atggccggcc gcacgtacca
    10081 ggcctccggc gcgtcttcgg gatagagccg ctcgccgccc tcgagccggc cgccctcccc
    10141 tcgggcctgc tcaagcgccg cctgcatcgc atcgaagccg gcgccatcga tgagcgggcc
    10201 gaccaggacg ccgtcggcca gcggattgcc gaccggcacg ccggcccaga cctgcttcag
    10261 gcgggggacg agttcgtcat agacttcgtc gtggacgaac agccgccgca atgtggtgca
    10321 gcgctggccg gcggtgccca ctgcggcgaa ggtgatcgcc cgctccgcca gatcgaggtc
    10381 ggcggtcggc gccaccacca tgccgttatt gccgcccagc tccagcaagc tgcgcccgaa
    10441 cctcgccgcc acccgcggcg cgatctcgcg gcccatccgg gtcgagccgg tcgccgagac
    10501 cagagcgacg cggcgatcgt cgaccagcgc ctccccggct gcgcggccgc cttggacgac
    10561 catggacaga tgcgcaggcg cgtccccgaa ccgttcggcc gcacgctcga acagcgcctg
    10621 cacggccgcg ccgcagagcg gcgtcttctc cgagggtttc cagacgacgc cgtcgccgca
    10681 gacgaaggca aggcaggcgt tccacgccca taccgccacg ggaaagttga aggcggagat
    10741 cacccccacg acgccgagcg ggtgccaggt ctccatcatc cggtgccccg gccgctcggt
    10801 ggcgatggtg aggccgtaga gctggcgcga gaggccgacc gcgaagtcgc aaatatcgat
    10861 catctcctgg acctcgccga gaccctcctg aaggatcttg cccgcctcga gactgacgag
    10921 ccggccgagc gtctccttct cctcgcgcag cacctgcccg aacaggcgca ccagctcgcc
    10981 gcggcggggc ggcggaacga ggcgccaggc gagatgcgct tggtgggcct gctccaccgc
    11041 ttcggccacg tcgcccgcgc cttgctcggc gacccggccg atcgtcctgc cgtcgatcgg
    11101 cgagcgggcc tcgatcgcgc cctcccgcgg cagttggagg ccgagtcgct cgaacagccg
    11161 ctcggtctcc gcgcgggaat cgaaggatgt gaaattattc aaggcggcct ccgctggctg
    11221 gcgacggacg cccctatcaa gcggcggcgg ccgcacaagg ggcgtctccc tgtcctgggg
    11281 cgacagcgcg ggcccgtctg ttctattgta agaagctcgc ttcgccttta tggcgtcagc
    11341 gaaggaaaga cgccgtcgcg gctacgcggc ggcttttttg ggggtcgcgg acgttgcctt
    11401 tcggcaagcg gcaggatcgc ctctttccgc ccattgccca cgagaccagc gtgacccagc
    11461 tattgcaagc ccgcgagttc gacatgaatg cagccgccga gccgagccgc ctcacccacc
    11521 tgcagcggct ggaggcggaa agcatccaca tcctccgcga agtcgcggcc gaggccgaaa
    11581 agccggtgat gctctattcg atcggcaagg attcggcggt gatgctgcac cttgcgcgca
    11641 aggccttcta cccggcgccg ccgcccttcc ctctgctcca cgtcgacacg acctggaagt
    11701 tcagggcgat gtacgagatg cgcgagcgcg ccgcccagga ggccggcatg gagctcctcg
    11761 tccaccacaa tccggaggcc aaggagaagg ggatcaaccc gttcgaccat ggtccgctcc
    11821 acaccgacat gtggaaaacc gaagggctga agcaggcgct cgacaaatat ggcttcgacg
    11881 cggccttcgg cggcgcgcgg cgcgacgagg agaagagccg cgccaaggag cgggtattct
    11941 cgttccgatc ggccaaccac cgctgggacc cgaagaacca acgccccgag ctgtggcggc
    12001 tctacaatgc ccgcaaggcc aaaggcgaga gcatgcgggt gttcccgata tccaactgga
    12061 ccgagctcga cgtgtggcaa tatatccacc tcgagaatat cccgatcgtg ccgctctatt
    12121 tctcggcgcc gcgcccgacc gttgagcgcg acggcctgct cctgatggtc gacgacgacc
    12181 ggttccggct gcgcgagggc gaacagccgg tcgagcggtc ggtgcgcttc cggacgctcg
    12241 gctgctatcc gctcaccggc gccgtcgaga gcgaggcgaa gacgctgccc gacattattc
    12301 gcgagatgct gctcaccacc acctcggaac gccagggccg cgcgatcgac cacgaccagg
    12361 ccgccagcat ggagaagaag aagcaggagg gctatttctg atggccaccc aggttcagga
    12421 atcctcctac aaggcggacg agctcatcgc cgaggacatc ggcgcctatc tggagcagca
    12481 ccagcataag agcctgctgc gcttcatcac ctgcggcagc gtcgacgacg gcaagtcgac
    12541 cctgatcggg cggctgctct acgattcgaa gatgatcttc gaggaccagc tcgccgccct
    12601 cgaagccgat tccaagcggc acgggacgca ggggcaggag atcgactttg ctttgctggt
    12661 cgacgggctc gccgccgagc gcgagcaggg tatcaccatc gacgtcgcct accgcttctt
    12721 cgccaccgac aagcgcaagt tcatcgtcgc cgacacgccc ggccacgagc aatatacgcg
    12781 caacatggtg accggcgcct cgaccgccga gctcgccgtg atcctgatcg acgcgcgcaa
    12841 gggtgtgctg acgcagaccc ggcggcacag ctttctggtc aatctgatcg gcatcaagaa
    12901 cgtcgtcctc gccatcaaca agatggatct cgtcgattac agccaggccc ggtacgaaga
    12961 gatactggcc gaatatcgcg cctttgcgag cgagatcggg atcggcaatt tcgttgccat
    13021 ccccatttcg ggcctgcgcg gcgacaacat caccgagcgg tcggcgacaa tgccctggta
    13081 tgtcgggccg accttgctgg cgcacctcga gacggtgcag ctcgacgagg agcatctgcg
    13141 cacctcctcc ttccggatgc ccgtccagtg ggtcaaccgc cccaaccacg aatttcgcgg
    13201 cttcagcggc atgatcgccg gcggcacggt ccgcaaatat gacgcggtgc gggtgctgcc
    13261 ttcgggccgc acctcgaccg tgagccggat cgtcaccctc gacggcgacc tcgacagcgc
    13321 cgttgccggc cagtcggtca caatcacgct cgccgacgag gtcgattgct cgcgcggcga
    13381 cgtcatcgtg gcggcggacc agccgctcga agtcgccgac cagttcgaag cgacgatcgt
    13441 gtggatggac gacgaggcga tgctgccggg gcggccttat tggctgaaga tcggtaccac
    13501 caccgtcagc gccaccgtca acgagcccaa ataccaggtc aacgtcaaca cgctcgagca
    13561 gctcgccgcc aagacgctcg aattgaacgc gatcggcgtc gccaatctgc agctcgaccg
    13621 ggcgatcccg ttcacgcctt atgcggaaaa tcacgacctc ggcggcttca tcctggtcga
    13681 caagctgacc aacgccacgg tcggcgccgg catgatccat ttcgcgctcc gccggtcgca
    13741 caacatccac tggcagcatc tcgaggtcga ccgcaacgcc catgccaggc tcaaggggca
    13801 gaaggcgaaa gtgctgtggt tcaccggact ttcgggcgcc ggcaaatcga ccatcgccaa
    13861 tctcgtcgag aagaagctgc acgacatggg gcggcacacc ttcctgctcg acggcgacaa
    13921 tgtccgccac gggctcaaca aggatctcgg cttcaccgac gccgaccggg tcgagaatgt
    13981 ccgccgcgtc ggcgaggtcg cgcggctgat ggccgatgcc gggctgatcg tgatgtgcgc
    14041 gttcatctcg ccgttccggg ccgagcggcg gatggtccgt gagatgatgc cggagggcga
    14101 gttcgccgaa gtctgggtcg acaccccgct ggccgaagcg gagcgccgcg acgtcaaggg
    14161 gctgtacaag aaggcgcgca gcggcgcgct cgccaatttc accggcatcg acagccccta
    14221 tgaggagccg gaaaatgccg agctccggat cgacaccacc aagatgagcc cggaagaagc
    14281 ggccgagcat atcgtcgcag agctgctgcg atgaccgatg ccgaccttgc cgcccgcatc
    14341 gccgagggcg cgggcgcgat cctgctcgcg gtgcgcgccg ccgggctgat cgaaggcaag
    14401 gcgctgggcg cggcgggcga ccggatcgcc aacgccttca tcctcgacgc gctgcaggcg
    14461 cagcggccgg acgacgcggt gctttcagag gagagcgtcg acacgaccga tcggctggag
    14521 caggagcggg tctggatcgt cgatccgctc gacggcaccc gcgaatattc cgaaggacgc
    14581 gaggattggg cggtgcatat cgcgctggcg atcggcggcc gcccggcgct gggcgcggtc
    14641 gcgctgccgg cgcagggact ggtgctgcga tcggatgcgc cgccggcgtt gcccgcgctt
    14701 gcggacgggc cggtgcggct gctggtcagc cgcacgcgcc cgcccgccga agccgaacgg
    14761 gtcgccgaag cgctcggcgc cgagctcgtg ccgatgggtt cggccggggc gaaggcgatg
    14821 gcggtgctgc tcggccaggg cgacgtctat ctccacgcag gcgggcagta tgaatgggac
    14881 aactgcgcgc cggccgcggt ggcgctcggc gccggcctcc acgccacccg gctcgacggc
    14941 tcgccgctcg aatataaccg ggccgacccg aagcttcccg acctgctcat ctgccaccgc
    15001 agccttgccg agcgcgtccg ggccgcgctc gcctgacctc gatggcgggg cagcgcatcc
    15061 agccggtgat cctctccggc gggtccggca cccggctctg gccgctgtcg gtgccggagc
    15121 gccccaagca attgctggcg ctgaccggcg aagaaaccat gatccagctt accgccgagc
    15181 gggtgcggga cgcggagcgc tatgcggcgc cgatcgcggt gtgcggcgag cgccatgccg
    15241 atcaggtcga ggcgcagctg accgcgatcg gcaccccccc ggcgctggtg atcgtcgagc
    15301 cgagcgcccg caataccgcc cccgccatcg cgctcgcggc gctggcggcg gctccggacg
    15361 attgcctgct ggtgatgccg agcgaccatg tcgtcgcgga cgtggcggcc tttggcgcgg
    15421 tcgtcgagcg ggcgctcgcc gccgccgcgg atggctggat cgtcaccttc ggcatcgagc
    15481 ccgatcgccc ggagaccggc tatggctata tcttccgcgg ggaggccctc gccgagggtg
    15541 tcaacgaggt cgagcgtttc gtcgagaagc cggaccgggc gacggccgaa gcctatctgg
    15601 cggcggggca atgttcgtgg aacagcggca tcttcctgtt ccgggcgggc gactatctgg
    15661 aggcgctgga gcggcacgcc cccgacatat tggcggcggt gcgggctgcg gtggccgggg
    15721 gacggcgcga agccggccgg ctctaccccg aggcgagcgc gttcgcccga tcgccgtcca
    15781 tctcgatcga ctatgcggtg atggaaaaag tggagcgggc ggcggtggcg ccggtcagca
    15841 tgggctggtc ggacatcggc agctgggacg cgctctacga cctcatagag aaagatgcgc
    15901 atgggaacgc cctgtctggg gaggtcgtcg cgatcggcgg cagcggctcg ctgatccgca
    15961 cgaccgggcc gaaagtggcg gcgatagcgg tcaatgattt gatcatcctg gcgacgccgg
    16021 aggcggtgct ggtcgtgccc cgaggggaga gccagcgggt caaggaggcg gtcgaggcga
    16081 tcgagcggct tgggggcggc gcgaagtaga agggagatcc cagctttcgc tgggatgacg
    16141 cttactcggg ccgttacgac ttcaacaggt cggcgcgggc cttgggctcg gcgtaggcga
    16201 tgaggaggtc ggcgccggga tcgagggcga gggcggtttc ggcctcgacc atccagactt
    16261 cgcccggctc gagcggctcg ccggcgaccc ggccggtgcc ggcgacgggg atcagccaca
    16321 gcggagcggc ggcggcgctg agccggccct cgatgagccc agtccagcgt tcgaggacga
    16381 aggccgggcc ttcggcgagg atcaggcgcc cctcgccttc cgcgcgcggc gcgaacggcg
    16441 gctcgtaagg gaccggattg gcgaccgcga tgctctcgtc gaggtgaagc tcgcgcgggc
    16501 ggccataatc gtagagccgg taggtgaggt cgacattctg ctggacctcg atcagcgcca
    16561 acccctggcc gagcgcgtgg atggtcccgg caggggaata atagacgtcg ccggccgcgg
    16621 ccggacgcca atcgagcaat tgctcgatcg acccgtcgag cgcggcggcg cgcagctcat
    16681 cgggcgtgac cggacggcgc agcccgatgc cgatcaccgc gcccgcgtcg gcgcccagca
    16741 ccacccacgc ctcgtccttg ccgctcggat agccggcggc gcgcgcgtcg gcatcgcccg
    16801 gatggacctg gatcgagagc ttttccgagg tgaacagata tttgaccagc agcggccggg
    16861 cggcgccggc cggatcctcg aaccagattt cgccgaccgg ctccgcttcg tacggtaccg
    16921 taccgaacat cgccaggtcg cgccgccccc agggcttttc gacccgcttc ggtgtcagcc
    16981 ggaccgccgt cactccactt caccccgcta gttggcgtct ggaccgaaac aatgctaagg
    17041 cggtccatag acaagcctgc ccgtctagcc aacggtgcag attcgtcgcc tagacggggg
    17101 cattggtcgc ggcgcgcttg agagcagggc cttaagcctt tgtgtttttg cctgtttcgc
    17161 cctagggtga atcgccagtt ctggacagct tgtaagggga aggcgtggcc agctcatcgt
    17221 tcttggtcga tcggggcgga agcgagcccc tcgtcctgcc gcagtcggcg gaggagcagc
    17281 attcgttcgt ccacgcggta cgggcgctcg gcagctccaa ggaactgctc cggctgaagc
    17341 tttactcgtc gctgatggcg gtcgacattg ccggcctggc gctcgccttc atgttggcga
    17401 gctggatccg cctcggcagc ccgctgcacg gcgaaggcat cgacacgctg gcggtggtgc
    17461 tgccgctgtt catcggaatc gcgctcaaca gccgggccta ttcgatcgaa gtgctgaagg
    17521 cgccgcgagt cggcatccgc cgcgcgctcc agtcgctggt gttcgcgctg gtcgcggtga
    17581 tcggcatcat cttctacttg aaggcgagcg agaccttctc gcgcctgctg tttgcgatcg
    17641 gcggcgcgag cgcggtcggc ttcgtcgcgc tcgggcgctg gggcctcggc catttcgccg
    17701 gccgccgctg cggctggagc ttcaccaacg aagtgctgct ggtcgacggt ctgcaggtgt
    17761 tcccgcgcga cggcgagatc gtgctgttcg ccgaccaggt gaacctcagc ccgcgcaccg
    17821 acgaccccga attgctcgac cggatcgggc ggctgctcaa gaattgcgac cgcgtgctgg
    17881 tcgcctgccc ggccgagcgc cgcgccgcct ggtcggcgat gctgaaaggc accgacgtcg
    17941 acgtcgaagt gctggtgccc gagctggacg gcctcggcgc gctcgacctc aggcggttcg
    18001 gcgaccgttc gaccgtgctg gtcggctgcg gcccgctcgg gcttcgcgat cgcgtcgcca
    18061 agcgcgggct ggaccttgcc atcgcactgc ccgccaccct gctgctgctg ccgctgatgc
    18121 tgatcgtggg cattgccatc aagctcgaat cggccggccc ggtcttcttc agccagccgc
    18181 gggtcgggcg cggcaatcgc atcttccacg tgctgaagtt ccgcagcatg cgcgccgatt
    18241 ccgccgattc cgccggggtc cggtcgaccg cccgcggcga cgaccgcatc acccggatcg
    18301 gcgccttcat ccgccgcacc agcatcgacg agctgccgca gctgatcaac gtggtgctgg
    18361 g
//
LOCUS       DASVRK010000211         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_105154, whole genome shotgun sequence.
ACCESSION   DASVRK010000211 DASVRK010000000
VERSION     DASVRK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105154"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VF589_08360"
     CDS             complement(<1..750)
                     /locus_tag="VF589_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8257634.1"
                     /translation="MQAAFDGRAALLASNSPQTIESHRVLGSWSRPADLSGFNEGINS
                     GHALDTKLGYPRRMAPALTSTARIIVVDDDDSVRDALLEYLSGRGYGVRAAAHAPAFD
                     ALWAAERAELVIVDVMMPGEDGLSLCRRLAPLGVPILMLSALDGVTDRVVGLETGAWD
                     YLSKPFEPAELLARVRALLRRPGSGGAAARGQEEVTFAGWWLDVAQRRLRDPAGRPLS
                     LTASEHALLAVFVERPGRVLSRDALLDAARGL"
     gene            complement(1065..1292)
                     /locus_tag="VF589_08365"
     CDS             complement(1065..1292)
                     /locus_tag="VF589_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX8257635.1"
                     /translation="MANGQVTYKVVIEPLSEEDGGGYLATVPELPGCMSDGETPAEAL
                     VNVQDAIATWIHCARKMGRTIPEPQLHRSAA"
     gene            complement(1294..1548)
                     /locus_tag="VF589_08370"
     CDS             complement(1294..1548)
                     /locus_tag="VF589_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX8257636.1"
                     /translation="MARGNKLLDRMRANPRDWRIEDVGTLCGAFGLDFDRPPGGSHYG
                     VSDPTQEMHVTIPFARPIKPVYIRRLVKYVDAVLAAREDG"
     gene            complement(1578..2285)
                     /locus_tag="VF589_08375"
     CDS             complement(1578..2285)
                     /locus_tag="VF589_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8257637.1"
                     /translation="MTLRELVIAYVQYPAIIAYFALSVAALGLFALKPAPLLPTLVAA
                     VAAAVAYPLVWYVLHRWVLHSRWMFKVPLLASTWKRIHYDHHQDPNHLEVLFGALHTT
                     LPAIAIASAPLGYLIGGFGGACIAFATGLLTTCFYEFCHCIQHLAYKPKSRWLAEMKK
                     RHMAHHFHDETGNFGITTFFWDKLFGTYYDRTERPAKSPTVFNLGYTPEVAEKWPHVA
                     QLSGGVATGHPRERVRK"
     gene            complement(2469..>3332)
                     /locus_tag="VF589_08380"
     CDS             complement(2469..>3332)
                     /locus_tag="VF589_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257638.1"
                     /translation="SLALTALLCVRLLARFFDLRLLLRAPVDAHLVWSLLGTGLALLP
                     GARGATAAGLFEIARKIATVPLIVRQAFHYVMSPLASAQARADRAAIAPLYRFACRVS
                     AALVVPLAGLVIFAGADILSVYRPEAAAALPLLVILVLARSAEAILGPAVAIVEMTGH
                     RALPLINSAAAAALWAALAWWLVPGSGATGMAIATGAATFLAVAAAAAELRLTERLSP
                     FDRRLFAALGLALAGVALMALAESLAGGPARFASILLIWAATSWCALRFGLQLGDREA
                     LGGLARRLKLV"
BASE COUNT          585 a         1068 c         1207 g          472 t
ORIGIN      
        1 cagccctcgc gccgcatcga gcagcgcatc ccggctgagc acccgccccg gccgctcgac
       61 gaagacggcg agcaaggcat gctcgctcgc cgtaagcgac agcggccggc cggcggggtc
      121 ccgcagccgc cgttgggcga catcgagcca ccagccggca aaggtcactt cttcctgccc
      181 ccgcgccgcc gcgccgccgc tgcccggccg ccgaagcagc gcccgcaccc gcgcgagcag
      241 ctcggccggt tcgaacggct tggaaagata gtcccacgcg cccgtttcca ggccaaccac
      301 tcggtccgtc acgccgtcga gcgcgctcag catcaggatc gggacaccga gcggggcgag
      361 ccggcggcag agcgacagac cgtcttctcc gggcatcatt acatcgacga tcaccagctc
      421 ggcgcgttcg gccgcccaca gggcgtcaaa ggccggggcg tgggccgccg cgcgaacgcc
      481 gtagccacgc ccgctcaaat attcgagcag agcgtcacgg acgctgtcat catcgtcaac
      541 gacgatgatc cgtgcggtgc tggtcagggc gggagccatg cgccgaggat accccagctt
      601 tgtatcaagg gcatgtccag aattgatacc ttcgttaaag ccgctcaggt ccgcaggtcg
      661 agaccaagag ccaagaaccc ggtggctctc gatcgtttgc ggtgagttgg aagccagcaa
      721 agcagctcgc ccgtcgaagg cggcctgcat cgctgagtct gcttcagagg tgtcgagcag
      781 ataggctgga cggcagcttc tgcgctaagc tgacaaccgg tcagccttcg cccgctcccg
      841 cggatgcccg gtcgcgacgc ccccggacag ctgcgccacg tgcggccatt tctcggccac
      901 ttcgggcgtg taggccaggt tgaagaccgt cgggctccct tggtcgcctc aggacaggct
      961 tcgccggccg ctccgtgcgg tctgccggga tccccgccca gcacttcgcg ggggtggcta
     1021 tcaagtgccc atcagtcctg cagcttgatc ggcggcgcct cgcgtcaggc cgcggagcgg
     1081 tgaagctgcg gctccggaat ggttcgcccc atcttgcggg cgcagtggat ccacgtggcg
     1141 atcgcatctt gaacgttgac aagggcttcc gctggcgttt ctccgtcgga catgcagccg
     1201 ggcaactccg gcacagtcgc gagatagccc ccgccatcct cttcggacag cggctcgatc
     1261 accaccttgt aggtcacctg gccgttcgcc acctcaacca tcctctctgg ccgcgagcac
     1321 cgcgtccaca tacttgacga gcctccgaat atatactggc ttgatcgggc gggcgaaggg
     1381 aatcgtgacg tgcatttctt gcgtcggatc gctgacgcca tagtgagagc cgcccggagg
     1441 acgatcgaaa tcaaggccga acgcgccgca cagcgtgccg acatcctcga ttcgccagtc
     1501 gcgcggattg gctcgcatgc ggtcgagaag cttgttgccg cgagccacgc gccaggaccg
     1561 cgccccgctc ggaagcccta cttccgcact cgctcccgtg gatgccccgt cgccaccccc
     1621 ccggacagct gtgccacgtg cggccacttc tccgccacct cgggcgtgta gcccaggttg
     1681 aacaccgtcg ggctcttcgc cgggcgctcg gtgcggtcgt aataggtgcc gaagagcttg
     1741 tcccagaaga aggtggtgat tccgaaattg ccggtctcgt cgtggaaatg atgggccatg
     1801 tggcgcttct tcatctcggc cagccagcgg ctcttcggct tgtaggcgag atgctggatg
     1861 cagtggcaga attcataaaa gcaggtggtg agaaggccgg tcgcgaaggc gatgcaggcg
     1921 ccgccgaagc cgccgatcag atagcccaag ggcgccgagg cgatggcgat cgccggcaaa
     1981 gtggtgtgga gcgcgccgaa caggacttcg aggtggttgg ggtcctggtg gtggtcgtaa
     2041 tggatgcgct tccaggtcga ggcgagcaga ggcaccttga acatccagcg gctgtgcagc
     2101 acccagcggt ggagcacgta ccacaccaac ggataagcga ccgcggccgc gaccgcggcg
     2161 acgagggtcg ggagcagcgg tgccggcttc agcgcgaaca ggccgagcgc ggcgaccgag
     2221 agcgcgaaat aagcgatgat cgccggatat tggacatagg cgatgacgag ctcgcgcagc
     2281 gtcatcttgt cgagatggtg cgagcgcttc caaaagccgg gacgcgtgga ttgggcgagc
     2341 ttcactctgt catacctcgg aaaaagggcc gtacctcgga aaattgccgc gggcgcacgg
     2401 gagccgaagc tcctgcacat ccatatgtgt ccttgtcatg aattcgcaat gaggcggatt
     2461 ttgggcgctc agacgagctt gaggcggcgg gcgaggccgc cgagcgcctc gcggtcgccg
     2521 agctggaggc cgaagcggag cgcgcaccag ctggtcgccg cccagatgag gaggatggag
     2581 gcgaagcggg cggggccgcc ggcgaggctt tcggccagcg ccatcagggc gacgccggca
     2641 agcgcgaggc cgagcgcggc gaacaggcgg cggtcgaacg gcgacaggcg ctcggtcagg
     2701 cggagctcgg cggcggcggc ggcgacggcg aggaaggtgg cggcgccggt cgcgatcgcc
     2761 atgccggtcg cgcccgagcc cggcaccagc caccaggcga gcgccgccca gagcgcggcg
     2821 gcggcggcgc tgttgatgag cggaagcgcg cggtggccgg tcatctcgac gatcgcgacg
     2881 gcgggcccga ggatggcttc ggcggagcgg gcgaggacga ggatgacgag caaggggagg
     2941 gcggcggcgg cctcgggccg gtagacgctc aatatgtcgg cgccggcgaa gatgacgagg
     3001 ccggcgagcg ggacgaccaa agcggccgag acgcggcagg cgaaacggta gagcggggcg
     3061 atggcggcgc gatcggcgcg ggcctgggcg gaggcgagcg ggctcatcac ataatggaag
     3121 gcctgacgga cgatcagcgg tacggtcgcg atcttgcggg cgatctcgaa caggccggcg
     3181 gcggtggcgc cgcgggcgcc gggcaggagc gcgaggccgg tgccgagcag gctccagacg
     3241 aggtgggcgt ccacgggggc gcggaggagc aggcgcaggt cgaagaagcg ggcgaggagg
     3301 cggacgcaga gcagggcggt cagcgccagc ga
//
LOCUS       DASVRK010000212         6260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_541547, whole genome shotgun sequence.
ACCESSION   DASVRK010000212 DASVRK010000000
VERSION     DASVRK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541547"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VF589_08385"
     CDS             complement(<1..1302)
                     /locus_tag="VF589_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8257639.1"
                     /translation="MPSFARELEQTLHTALAEASGRKHEYATLEHLLLALIEDSHASK
                     VMSACGVDLGELREAVRLYLDNELEALKVEGDTDPSPTSGFQRVVQRAILHVQSSGRD
                     EVTGANVLVALFSERESYAVYFLQQQDMSRLDAVSYISHGVGKGETSSEPPEVKGTEE
                     TKQEDGKKKSESALKQFTVNLNEKALDGRVDPLIGRGPEVDRTIQILCRRSKNNPLYV
                     GDPGVGKTAIAEGLARKIVEGQVPDVLKPAVIYSLDMGALLAGTRYRGDFEERLKAVV
                     TELEKLPDAILFIDEIHTVIGAGATSGGAMDASNLLKPALSGGTIRCIGSTTYKEFRN
                     HFEKDRALLRRFQKIDVNEPTVEDTIKILAGLRSAFEGHHSVKYTPDAIKSAVELSAR
                     YINDRKLPDKAIDVIDEAGASQMLVTEAKRKKTIGVKEIEAV"
     gene            complement(1427..1624)
                     /locus_tag="VF589_08390"
     CDS             complement(1427..1624)
                     /locus_tag="VF589_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX8257640.1"
                     /translation="MDLDELFPNKPSDPLSQLAAQDLDPLSVDELHERIAALEAEIAR
                     TKAKAEASVSFRADADAIFKK"
     gene            1707..2720
                     /locus_tag="VF589_08395"
     CDS             1707..2720
                     /locus_tag="VF589_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX8257641.1"
                     /translation="MNTATTPIRTTMMAIDPDEAGGPEVLKPVERPVPKPGPGEVLIE
                     VAAAGVNRPDVMQRLGRYPPPPGAPTIPGLEVAGRVVAVGAGELPQWIGKPVCALVSG
                     GGYAEYCTAPSGQCLSVPGTFTMVEAAALPETFFTVWANLFHHSYLGREDTLLVHGGT
                     SGIGIAALLLGQIFELNVIVTAGSDEKCRRALEQGAAWAINYRTHDFVEEVRRLMEGR
                     GVDVVLDMIGGDYVARNLACVADEGRHVSIAAQRGATAEIPLWEVMRRRLTLTGSTLR
                     ARSAEYKATLASEIRREVWPHLEVGRIRPVIDSVYPLARAAEAHARMESSEHVGKIVL
                     QVK"
     gene            2985..3866
                     /locus_tag="VF589_08400"
     CDS             2985..3866
                     /locus_tag="VF589_08400"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX8257642.1"
                     /translation="MKALAGELGPADLVRKRLSKVDKAVGPSGAPRRRYRTIWISDVH
                     LGTRGCNDRLLIDFLDHVDSDTLYLVGDIIDGWRMKKRYYWPERHNAIVRRIMKRAKR
                     GTEVVYIPGNHDEMFRQFAGMNFGGVRIRNKAVHVTADGRRLLVLHGDEFDAVVMAHK
                     WLAFAGDAAYEFLMKLNVVVNAVRRRLDLPYWSLSKHAKRKVKNAVEFISRFEEAVAH
                     EARAKKVDGVVCGHIHTAEERRYGDIVYYNDGDWVESCTALVEHGDGRMEILHWADEI
                     AARKAPPAAAAPFQAAA"
     gene            3866..4885
                     /locus_tag="VF589_08405"
     CDS             3866..4885
                     /locus_tag="VF589_08405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8257643.1"
                     /translation="MRILIVTDAWAPQVNGVVRTLQSVRSELEAAGHRVDLIAPDQFA
                     SIPCPTYPEIRLAIARGRSVGERIAAAAPDAIHIATEGPLGLAARRWCLRRGIPFTTA
                     YHTQFPDYLAGRTRLPAGLFWRYIRWFHAPARAVLVSTPTMARQLAAHGLPHTRHWGR
                     GVDLSVFRPDAPPHPALVEVRRPLNLYVGRVSAEKNIGAFLDCPCPGSKVVVGDGPAL
                     AGLKRRYPEAAFMGALHGAELASAYAAADVLVFPSRTDTFGLVMIEALACGTPVAAYP
                     VPGPVDVLRGGVGAIDPELGAAVAAALRCSRAECAAYGATFSWAASARQFAAALVPWK
                     AADAA"
     gene            5014..5709
                     /locus_tag="VF589_08410"
     CDS             5014..5709
                     /locus_tag="VF589_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HEX8257644.1"
                     /translation="MAQPLMPHATASWLVDNTALTFEQIADFCGLHILEVQAIADDTA
                     ATKLTGRDPLRAGELTQEEIERGQADPGYRLRIQKEPDKVRRTKGPRYTPVSKRQDKP
                     DGIAWIIRNHPEVTDGQMSKLIGTTRTTISAIRDRSHWNIANIQPKDPVTLGLTSQRE
                     LDAVVAKAAKAAGFEAPQDTRLAGDREALIEELRREREQHHRDAEAALAAEAAGEPAD
                     AGADQGFVDPFRS"
     gene            5687..>6260
                     /locus_tag="VF589_08415"
     CDS             5687..>6260
                     /locus_tag="VF589_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8257645.1"
                     /translation="MSIPSGAEPSARSNAGGATLDVSLADAGEAYSHKGLTYPFGRGG
                     PEPGQVIALAEGVGWTRLPVPGSLKHINIWLLDDGEAGGIAIVDTGLDSPVSREGWEA
                     LLVGPLAGRRVTRVLCTHFHPDHVGLAGWLTRRFAAPLWMSREEWLFARLLTADIRDA
                     PPPEAVAYWRAAGWSEERIGAEAEKGWGRFA"
BASE COUNT          962 a         2172 c         2097 g         1029 t
ORIGIN      
        1 cacagcctcg atttccttga cgccgatggt cttcttccgc ttggcctcgg tgaccagcat
       61 ctggctggcg ccggcttcgt cgatcacgtc gatcgccttg tcgggcagct tgcggtcgtt
      121 gatgtagcgc gccgacagct cgaccgcgga cttgatcgca tccggcgtgt atttgacgga
      181 gtgatgcccc tcgaaggccg agcgcaggcc ggcgaggatc ttgatcgtgt cctcgaccgt
      241 gggctcgttg acgtcgatct tctggaagcg ccggagcaaa gcccggtcct tctcgaaatg
      301 gttgcggaac tccttgtagg tggtcgagcc aatgcagcgg atcgtcccgc ccgacaaagc
      361 gggcttcaag aggttcgacg catccatggc gccgccggac gtggcgccgg cgccgatcac
      421 ggtgtggatc tcgtcgatga acaggatcgc gtcgggcagc ttctcgagct ccgtcaccac
      481 cgccttcagc cgctcctcga aatcgccgcg gtagcgggtg ccggcgagca gcgcccccat
      541 gtcgagcgag tagatcaccg cgggcttcag cacgtcgggc acctggcctt cgacgatctt
      601 gcgcgcgagg ccctcggcga tggcggtttt gccgacgccg ggatcgccca catagagcgg
      661 gttgttcttc gagcggcggc agaggatctg gatggtgcgg tcgacttcgg gcccgcggcc
      721 gatcagcgga tcgacccggc cgtccagcgc cttttcgttg aggttgacgg tgaactgctt
      781 cagcgcggat tcggacttct tcttgccgtc ctcctgcttg gtctcctcgg tgcccttgac
      841 ctccggcggc tcggacgagg tctcgccctt gccgacgccg tggctgatgt agctgaccgc
      901 atcgaggcgg ctcatatcct gctgctgcag gaaatagacg gcatagctct cgcgctccga
      961 gaacaaagcg acgagcacgt tggcgccggt cacttcgtcg cggcccgacg actggacgtg
     1021 gaggatggcg cgctggacga cgcgctggaa gccgctggtc ggcgagggat cggtgtcgcc
     1081 ctccaccttc agggcttcga gctcgttgtc gagataaaga cggacggcct cgcgcaattc
     1141 gccgaggtcg acgccgcagg cggacatcac cttggaggcg tggctgtctt cgatgagggc
     1201 gagcaacaga tgctccaacg tcgcatattc atgcttgcgg ccgctcgctt ccgcgagggc
     1261 ggtatggagc gtctgttcga gttcgcgggc aaatgacggc atttcaaact cctacctgcc
     1321 cgcgtgggtc acgggcgact tgccctcaat gtcgtgccgc gtcggcccag catcaagcac
     1381 gtctcacaaa taaccgggga gaaccgcgtc ggtttcgccg cggcgctcat ttcttgaaga
     1441 tcgcgtcggc atcggcgcgg aagctgaccg aggcttcggc cttggctttg gtgcgcgcga
     1501 tctcggcctc gagcgcggcg atgcgctcgt gaagctcgtc gaccgacagg ggatcgagat
     1561 cctgggcggc gagctggctc agcgggtcgc tcggcttgtt cggaaagagc tcgtccagat
     1621 ccatcgccgg cagaatcccc cggaacgggc gcttgtgtca acaagatcgg gcgggctacg
     1681 gccgaaagcc agcggagcgt gacccgatga acactgccac cacccctatt cgcactacca
     1741 tgatggcgat cgatcccgac gaggcgggcg gacccgaggt gctgaagccg gtcgagcggc
     1801 cggtcccgaa gcccggcccg ggagaggtgc tgattgaggt ggccgcggcc ggggtcaacc
     1861 gccccgacgt gatgcagcgc ctcggccgct acccgccgcc gcccggcgcg ccgaccatcc
     1921 ccggcctcga agtggccggg cgcgtggtcg cggtcggcgc cggcgaactg ccgcaatgga
     1981 tcggcaagcc cgtctgcgcg ctcgtgtcgg gcggaggcta tgccgaatat tgcaccgccc
     2041 cgtcgggcca gtgcctgtcc gttcccggca ccttcaccat ggtcgaggcg gcggcgctgc
     2101 cggagacctt cttcaccgtc tgggccaatc tcttccacca ttcctatctc ggccgcgagg
     2161 acacgttgct ggtccatggc ggcaccagcg ggatcggcat cgccgcgctg ctgctcggcc
     2221 agattttcga gcttaacgtc atcgtgactg cgggctcgga cgagaaatgt cgccgcgcgc
     2281 tcgagcaagg cgccgcctgg gcgatcaact accgcaccca cgatttcgtc gaggaggtgc
     2341 ggcggctgat ggaggggcgc ggcgtcgacg tcgtgctcga catgatcggc ggcgattatg
     2401 tcgcccgcaa cctcgcctgc gtcgccgacg agggccggca cgtctcgatc gcggcgcagc
     2461 gcggcgcgac cgcggagatt ccgctctggg aagtgatgcg ccgccgcctg accctcaccg
     2521 gctccacgct ccgcgcccgc tcggccgagt ataaggcgac gctcgccagc gagatccggc
     2581 gcgaagtgtg gccgcatctc gaagtcggcc gcatccgccc ggtgatcgat agcgtctacc
     2641 cgctcgcccg cgcggccgag gcccatgccc ggatggaaag cagcgagcat gtcggcaaga
     2701 tcgtgctcca ggtgaagtga cgatcgttct gaggtgaggt gacgcccccc gcggcgtcga
     2761 catgaggtga cgcccccccc cgcgccatcc ccgcgaaagc ggggatctca cttcttcttc
     2821 ctgtcgagac aactgccaga aaagcgattt cagcttgcgc tggaatgacg ttgttggctt
     2881 tcatcgtcac atcgatcgtc acggaaacgt cacaccgccc cgatccatat tgtcacaatc
     2941 aactgcgata cgctgacagt cgaacccacg aggggagacg gcgtatgaag gcgttggcag
     3001 gggaactcgg gccggcggac ctggtccgca agcgcctttc gaaagtcgac aaggcggtcg
     3061 ggccgtcggg ggcgccgcgg cggcggtacc ggactatctg gatctccgac gtccatctcg
     3121 gcacgcgcgg ctgcaacgac cggctgctga tcgacttcct cgaccatgtc gattctgaca
     3181 cgctctacct cgtcggcgac atcatcgacg gctggcggat gaagaagcgt tattattggc
     3241 ccgagcggca caacgcgatc gtccggcgca tcatgaaacg cgccaagcgc ggcaccgagg
     3301 tcgtctacat tcccggcaac catgacgaga tgttccgcca gttcgccggc atgaacttcg
     3361 gcggggtgcg gatccgcaac aaggcggtgc atgtcaccgc cgacggaagg cggctgctgg
     3421 tcctccacgg cgacgagttc gacgcggtgg tgatggccca taagtggctc gccttcgcgg
     3481 gcgacgccgc ttacgaattc ctgatgaagc tgaacgtggt ggtgaacgcc gtccgccgcc
     3541 gcctcgattt gccttactgg tcgctctcca agcacgccaa gcgcaaggtc aagaatgcgg
     3601 tcgagttcat ctcccgcttc gaggaggcgg tcgcgcacga ggccagggcc aagaaggtgg
     3661 acggggtggt ctgcggccac atccacaccg ccgaagagcg ccgctacggc gacatcgttt
     3721 attataatga cggcgactgg gtcgaaagct gcaccgcctt ggtcgagcat ggcgacgggc
     3781 ggatggagat actccactgg gctgacgaga tcgccgcccg caaagcgccg cccgccgccg
     3841 ccgcgccctt ccaggcggcc gcctgatgcg tatcctgatc gtcaccgacg cctgggcgcc
     3901 gcaggtcaac ggcgtcgtca ggacgctgca atcggtgcgg agcgagctcg aagcggccgg
     3961 ccaccgcgtc gacctgatcg cgcccgacca gttcgcctca atcccctgtc ccacttatcc
     4021 cgagatccgg ctggcgatcg cgcgcggccg cagcgtcggc gagcggatcg cggcggcggc
     4081 gccggacgcc atccacatcg ccaccgaagg gccgctcggc ctcgccgccc gccgctggtg
     4141 cctcaggcgc ggcattccct tcaccaccgc ctaccatact cagttccccg attatctcgc
     4201 cggccgcacc aggctcccgg ccggcctgtt ctggcgctac atccgctggt tccatgcgcc
     4261 cgcccgcgcg gtcctggtct cgacgccgac catggcgcgc cagctcgccg cgcacggcct
     4321 tccccacacg cgccactggg gccgcggcgt cgacctgtcc gtcttccgcc ccgacgcgcc
     4381 gccgcatccc gcgctcgtcg aggtccgccg cccgctcaac ctctatgtcg ggcgcgtctc
     4441 ggccgagaag aatatcggcg ccttcctcga ttgcccctgt cccggcagca aggtggtggt
     4501 cggcgacggg cccgcgctgg cggggctgaa gcgccgctac cccgaagccg ccttcatggg
     4561 cgcgctgcac ggcgccgagc tcgcctcggc ctatgccgcc gccgacgtgc tcgtcttccc
     4621 cagccgcacc gacacgttcg gcctcgtcat gatcgaagcg ctggcgtgcg ggacgccggt
     4681 cgcagcttat ccggtgccgg gaccggtcga cgtgctgcgg ggcggcgtcg gcgcgatcga
     4741 cccggagctc ggggctgctg tcgcggccgc gctccgctgc agccgggccg aatgcgcggc
     4801 ttatggcgcg accttcagct gggcggcgag cgcccgccag ttcgccgccg ctttggtccc
     4861 ctggaaggcg gccgacgcgg cctgattcgg gcgctccgtc ctcgcgcggg gcaactcttc
     4921 ccttgcctcg cgggggtggg cggattacat cgccggggca atgttgcggc cgctccggca
     4981 cggggcggcc cttattttat ctggaggatc aaagtggccc agccgctcat gccgcatgcg
     5041 accgcctcct ggctggtcga caataccgcg ctgacgttcg agcagatcgc cgacttttgc
     5101 gggctccaca tcctcgaagt gcaggcgatc gccgacgaca ccgccgccac caagcttacc
     5161 ggccgcgatc cgctccgtgc cggcgagctc acccaggaag agatcgagcg cggccaggcc
     5221 gatccgggct atcgcctgcg catccaaaag gagcccgaca aggtccgccg caccaaaggt
     5281 ccgcgctaca cgccggtgtc caagcggcag gacaagcccg acggcatcgc ctggatcatc
     5341 cgcaaccatc cggaagtgac cgacggccag atgtcgaagc tgatcggcac cacccgcacc
     5401 accatctcgg cgatccgcga ccgcagccac tggaacatcg ccaacatcca gcccaaggat
     5461 ccggtgacgc tcggcctcac ctcgcagcgc gagctcgacg ccgtcgtcgc caaggccgcc
     5521 aaggccgccg ggttcgaggc gccgcaggac acgcgtctcg ccggcgaccg cgaggcgctg
     5581 atcgaggagc tgcggcgcga gcgcgagcag catcaccgcg acgccgaagc ggcgctcgcg
     5641 gccgaagcgg ccggcgagcc cgccgacgcc ggtgccgacc agggctttgt cgatcccttc
     5701 aggagctgag ccctccgccc gcagcaatgc gggtggggcg acgctggacg tctcgctcgc
     5761 cgacgcgggc gaggcctata gccataaggg cctgacctac ccgttcgggc gcggcgggcc
     5821 ggagccgggg caggtgatcg cgttggccga aggcgtcggc tggacgcggc tcccggtgcc
     5881 gggctcgctc aagcatatca acatctggct gctcgacgat ggcgaggccg ggggcatcgc
     5941 gatcgtcgac accggcctcg acagcccggt ctcgcgcgaa gggtgggagg cgctgctggt
     6001 cggcccgctc gccggccgcc gcgtcacccg cgtcctttgc acgcactttc accccgatca
     6061 tgtcggcctc gccggctggc tgacgcggcg cttcgcggcg cctttgtgga tgagccggga
     6121 ggaatggctg ttcgcccgcc tgctgactgc cgacattcgc gacgccccgc cgcccgaggc
     6181 ggtcgcttat tggcgcgccg ccgggtggag cgaggagcgg atcggcgccg aagcggaaaa
     6241 gggctggggg cggttcgccg
//
LOCUS       DASVRK010000213         8054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_153109, whole genome shotgun sequence.
ACCESSION   DASVRK010000213 DASVRK010000000
VERSION     DASVRK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153109"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..381
                     /locus_tag="VF589_08420"
     CDS             109..381
                     /locus_tag="VF589_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HEX8257646.1"
                     /translation="MTIQDTNKGDAEALALGALGWTLAEPARAERLLALTGLTPADLR
                     ARIGEPPLLAAILRFLEAHEPDLIACADALGVAPARLASARREFEA"
     gene            423..1016
                     /locus_tag="VF589_08425"
     CDS             423..1016
                     /locus_tag="VF589_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8257647.1"
                     /translation="MLTHFADWVDEAHDLRFALDAPGFREALTHRDTGQVVAEERIWP
                     LLDGFFDSEMHRQNVVPGAVEALARIGEAADIVILTNIGDEYEAGRVRQLEAFDIRHR
                     VLCNRGGKGRPVVELIESMRPSVTVFVDDLPVHHESVAKHAPQVWRLHMIAEPIVAAH
                     MPAAPHAHARIDDWDAAADWILERFAEGQAPGRGERT"
     gene            1013..1765
                     /locus_tag="VF589_08430"
     CDS             1013..1765
                     /locus_tag="VF589_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8257648.1"
                     /translation="MSGTIFITGATAGIGAAAARRFAGAGWKVIATGRRRDRLDSLVA
                     ELGPERVHPVVLDITDHDAVDAALAALPAPFAGIDLLLNNAGLAPPMAPLHETELGAL
                     RRAIDTNIVALVALTRKLLPGLIARRGAIVNLSSVAATYPYRGGAVYGGSKAFVSQFT
                     LNLRSDLHGTGVRVTAIEPGLVKTEFTIVRTGGDVAASEALYRGIDPMTAEDIADTIF
                     WVASQPPHININSIELMPVTQSFAGFTVARED"
     gene            1920..4148
                     /locus_tag="VF589_08435"
     CDS             1920..4148
                     /locus_tag="VF589_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257649.1"
                     /translation="MLSSINYHLSLVDTFDLGALDGVSDAIGLSSGGFAVAGDRLLNG
                     DNETLIVDAAVNVTANTPDHVGLTPMLAELSNGNLVIAADDGLDIFFKIVDSAGNQVR
                     ASTGIGESGNDVLAPDVAGGLAGDRFVIVYVDNNGGQNNAVEFNIRNNDGSAGEGGTV
                     TALAGAHDLAPSVASLADGGFAVAWHRVVAGGTELRYAVYNSNGSVRLADTLFDGSGT
                     INRNASVVALDTGGFAIAYEDNSGGEGDIEISFARFGSGGAFVDSHNVFSNENDDTAP
                     TTARLANGLIAVGALNDSPLGGGSGIAILDETGAMRTALTGHSEGNNSFVGVAGLSLG
                     RVAQLYTDVETNEVAARVFNVVRSQASDDQGDRMVGTEFIDQLSAGGGDDVLRGGLNA
                     DILNGGSGNDAFEYDTFEVGAGEVIDGGAGTDRIHAFSTISLLRASLVSIERIDFAFD
                     VAQRIEISVLAEDFGNGLAADLRVGFGDPVLGHTLRVLMAEDTSLNLSRLQLGFFDPE
                     DFLDSRLIEVHGDGDAERITGTAIRDHISGAGGNDILAGGGAPDILRGGGGNDFYTVD
                     HQGEAIELAGQGTDTVRSDVAYTLGDNIERLQLTGAAGVTGRGNTLANILVGNDGANT
                     LNGGGGRDTLTGGGGGDRFLFTNRLDPAANVDRVTDFAAGVDDLRLDDAVFAGLAPGA
                     LAADAFRIGTRAIDASDRIVYNPETGALFFDADGTGGAAQIRFATLAPGIALTAADFL
                     VF"
     gene            4218..4685
                     /locus_tag="VF589_08440"
     CDS             4218..4685
                     /locus_tag="VF589_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8257650.1"
                     /translation="MTDDIAARLAGQNIVLPEPAAPVAAYVPAVRVGNLLHVSGQLPF
                     DDGKLMTGRLGEDKDVAYGYAAARACGIMLLAQIKKALGDLHKVERIVKLGVFVNSAA
                     GFTDQPKVANGASELMEQVFGEPGRHARSAVGVPSLPLGAAVEVDAIVAIKGS"
     gene            4657..5394
                     /locus_tag="VF589_08445"
     CDS             4657..5394
                     /locus_tag="VF589_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX8257651.1"
                     /translation="MRSSPSRAADRAGERLTELIALPFAHRGLHGGGIVENSRAAFAA
                     AIARGHGIELDVQASRDGDAFVFHDQSCDRLTLATGPIAARSSAELSRLELRGAGERL
                     ATLPDILALIAARVPLLIEVKTARDVAPLCRSVAGALADYRGAAGVMSFDPRVGAWFA
                     RHAPHRLRGLVVTEMNRKNLRGRMERRLALARARPHFLGYDIRDLPSPFAAAARARGI
                     AVCTWTVRTAAEGARAAEHADQIIYEE"
     gene            5413..6546
                     /locus_tag="VF589_08450"
     CDS             5413..6546
                     /locus_tag="VF589_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8257652.1"
                     /translation="MSGEILARIGDGVASFAAGDWDGCAGAGNPFLGHAFLSALEDSG
                     SASGRTGWQPVPIAIDGPDGRPAAVMPAYAKSHSQGEYVFDHGWASAYERAGGHYYPK
                     LQIAVPFTPVPGRRLLTAEPALAPALIKAAEAVVRSNGLSSAHATFIDPDEVRLFERA
                     GWLIRVDSQFHWHNRGYATFEDFLATLASRKRKAIRKERAAALAGLTVEHVTGGAIEE
                     RHWDHFWRFYQDTGARKWGRPYLNRRFFTLLGERMADRVLLIFALRDGVPIAGALNMI
                     GADTLYGRYWGATEEVPHLHFEICYYQAIDAAIARGLARVEAGAQGEHKLARGYEPVP
                     TYSAHYLLDPSLRAAVADYLESERRAVVNEIDILHGMTPFKKG"
     gene            complement(6533..7384)
                     /locus_tag="VF589_08455"
     CDS             complement(6533..7384)
                     /locus_tag="VF589_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX8257653.1"
                     /translation="MRLRSLLFVPGDRPDRMHKALGAGADALILDLEDAVAPAAKPEA
                     RRAVAEFLGANSAAPLWVRVNPLDGPECDRDLQAILGSHPHGIVLPKAEGGASVAELA
                     RRLTERGNVAAQILAIATETPAAMFQLGTYGGAKRLAGLTWGAEDLPAAIGASTSREP
                     DGRFTPPYELARSLCLFGAAAAGVAPIETVYPAFRDLDGLAAYAARARRDGFTGMMAI
                     HPDQVPVINAAFTPSPAEVEHARAIVAAFEAQPGAGALSLAGSMIDRPHLVQAQRVLA
                     AAGASPS"
     gene            complement(7381..7881)
                     /locus_tag="VF589_08460"
     CDS             complement(7381..7881)
                     /locus_tag="VF589_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8257654.1"
                     /translation="MTGRYYDEWTVGDRLQHQPHRTVTETDNLLISALTHNPQPLHLD
                     AEYAAGTEFGRIVVNGTFTFALMVGLSVGDTTLGTLVANLGYDKVRMPKPVFVGDTLR
                     AETEVIALKDSKSRPEAGIVTFEHRLLNQNGELVCVCERTALMQRRGPLHQPAAGPPP
                     RCGEET"
BASE COUNT         1230 a         2998 c         2637 g         1189 t
ORIGIN      
        1 cgcagcaggt cgcagaacag cttcagattg agttcgttgt cctcgacaac gaggattttt
       61 tgtcccaacg cgcctgcctt cggagaaagc cccccggcac cttaggcgat gacgattcag
      121 gacacaaata aaggcgacgc cgaagccctc gcgctgggcg cgctcggctg gaccctcgcc
      181 gagcccgccc gcgccgagcg gctgctcgcg ctcaccggac tcaccccggc cgacctccgc
      241 gcccggatcg gcgagccgcc tttgctcgcc gccatcctgc gtttcctcga agcccacgag
      301 cccgacctca tcgcctgcgc cgatgccctc ggcgtcgccc cggcccggct cgcttcggcc
      361 cgccgcgagt tcgaagcgtg aggccgctgc tcatctccga ttgcgacgag gtgctgctcc
      421 acatgctcac ccatttcgcg gactgggtgg acgaggcgca cgacctccgc ttcgcgctcg
      481 acgcccccgg cttccgcgaa gcgctcaccc accgcgacac cggccaggtc gtcgccgagg
      541 agcggatctg gccgctgctc gacggcttct tcgacagcga gatgcaccgc cagaacgtcg
      601 tccccggcgc ggtcgaggcg ctggcgcgga tcggcgaggc cgccgacatc gtcatcctca
      661 ccaatatcgg cgacgaatat gaagccggcc gcgtccgcca gctcgaagcc ttcgacatcc
      721 gccaccgcgt gctctgcaat cgcggcggca agggccgtcc ggtggtcgag ctgatcgaga
      781 gcatgcggcc gagcgtcacc gtcttcgtcg acgatctccc cgtccaccac gaatcggtgg
      841 ccaagcacgc gccccaggtc tggcgcctcc acatgatcgc cgaaccgatc gtcgccgcgc
      901 acatgccggc cgctccccac gcccacgccc ggatcgacga ttgggacgct gcggccgact
      961 ggatcctgga gcgcttcgcc gaagggcagg cgcccggccg aggagagcgc acatgagcgg
     1021 aaccatcttc atcaccggcg ccaccgccgg catcggagcg gccgcggcgc ggcgcttcgc
     1081 cggcgccggc tggaaggtga tcgcgaccgg ccgccgccgc gaccggctcg acagcctcgt
     1141 cgccgagctc ggccccgagc gcgtccatcc ggtggtgctc gacattaccg accacgacgc
     1201 cgtcgacgcc gcgctcgccg ccctgcccgc tccgttcgca ggcatcgacc tgctgctcaa
     1261 caatgccggt ctcgcgccgc cgatggcgcc gctgcacgag accgagctcg gcgcgctccg
     1321 ccgcgcgatc gacaccaata tcgtcgcctt ggtggcgctg acccgcaagc tgctgccggg
     1381 gctgatcgcg cgccgcggcg cgatcgtgaa cctcagttcg gtggcggcga cctaccccta
     1441 tcgcggcggc gccgtctatg gcggcagcaa ggccttcgtc agccagttca ccctgaatct
     1501 gcgctccgac ctccacggca ccggcgtgcg cgtcaccgcg atcgagcccg gcctggtcaa
     1561 gaccgaattc accatcgtcc gcaccggcgg cgacgtcgcc gcttccgaag ccctctatcg
     1621 cggcatcgac ccgatgaccg ccgaggacat tgccgacacc atcttctggg tcgcctcgca
     1681 gccgccgcac atcaacatca acagcatcga gctgatgccg gtcacccaat ccttcgccgg
     1741 cttcacggtc gcccgcgagg actgaccgca gccctacgcg gcgatgcggg cgaaacggct
     1801 cgtcccgtcc agaggcagct tggtcgcgca gcgttcgcca ggcagcgtcg ccggtctagg
     1861 ctcgcaggat agcagccgtc ccggccgagc ggagccggac cgacccaggg gggtgcgcca
     1921 tgctgagctc gatcaactat catctgagcc ttgtcgatac gttcgatctc ggcgccctcg
     1981 acggcgtcag cgacgccatc ggcctttcca gcggcggctt cgcggtcgcc ggcgaccggc
     2041 tcctgaacgg cgacaacgaa accctgatcg tcgacgccgc cgtcaacgtc accgccaata
     2101 ccccggacca tgtcggcctc acgcctatgc tggccgagct cagcaacggc aacctcgtca
     2161 tcgccgccga cgacggcctc gatatcttct tcaagatcgt cgactcggcc ggcaaccaag
     2221 tccgggcctc gaccggcatc ggcgagagcg gcaacgacgt gctcgccccc gatgtcgccg
     2281 gcggcctcgc cggcgaccgc ttcgtgatcg tctatgtcga caataatggc ggccagaaca
     2341 acgccgtcga attcaacatc cgcaacaatg acgggagcgc cggcgagggc ggaaccgtca
     2401 ccgccctcgc cggcgcccac gatctggcgc cgagcgtcgc cagcctcgcc gacggcggct
     2461 tcgccgtcgc ctggcaccgc gtcgtcgccg gcggcaccga gctgcgctac gccgtctaca
     2521 attcgaacgg cagcgtccgc ctcgccgaca ccctgttcga cggcagcggc accatcaacc
     2581 gcaacgcaag cgtggtcgcg ctcgacaccg gcggcttcgc catcgcctat gaggacaata
     2641 gcggcggcga aggcgacatc gagatcagct tcgcgcggtt cggttccggc ggcgccttcg
     2701 tcgacagcca caacgtcttc tcgaacgaga atgacgatac cgcgccgacc acggcccggc
     2761 tggcgaacgg cctcatcgcc gtcggagccc tcaacgacag cccgctcggc ggcgggtcgg
     2821 gcatcgccat cctcgatgaa accggcgcga tgcgcaccgc gctcaccggc cattccgagg
     2881 gcaataacag cttcgtgggc gtggccggcc tctcgctcgg ccgagtcgcc cagctctaca
     2941 ccgacgtcga aaccaatgag gtcgccgcca gggtgttcaa cgtcgtgcgc agccaggcga
     3001 gcgacgacca gggcgaccgc atggtcggca ccgagttcat cgaccagctc agcgcgggcg
     3061 gcggcgacga cgtgctgcgg ggtgggctca acgccgacat cctcaacggc ggctccggca
     3121 acgatgcgtt cgaatatgac acgttcgagg tcggcgcggg cgaggtcatc gacggcggcg
     3181 ccggcaccga ccgcatccat gccttctcca cgatcagcct gctgagggcg tcgctggtgt
     3241 cgatcgagcg gatcgacttc gccttcgacg tcgcccagag gatcgagatc agcgtgctgg
     3301 ccgaggattt cggcaacggg ctcgccgccg atctccgggt cgggttcggc gaccccgtcc
     3361 tgggccacac gttgcgcgtc ctgatggcgg aagacacgtc gttgaacctg tcgcggctcc
     3421 agctcggctt cttcgacccc gaagacttcc tcgactcccg cctcatcgag gtccacggcg
     3481 acggcgacgc ggagcggatc accggcaccg ccatacgcga ccacatcagc ggcgcgggcg
     3541 gcaacgacat cctcgccggc ggcggcgcgc cggacatatt gcgcggcggc ggcggcaacg
     3601 acttctacac cgtcgaccat cagggggagg cgatcgagct cgccggtcag ggcaccgaca
     3661 ccgtccgctc ggacgtggcc tatacgctcg gcgacaatat cgagcggctg cagcttaccg
     3721 gcgccgccgg cgtcacgggc cgcggcaaca ccctcgccaa catcctcgtc ggcaatgacg
     3781 gcgccaacac gctgaacggc ggcggcgggc gcgacacgct gaccggcggc ggcggcggcg
     3841 accggttcct gttcaccaac cggctcgatc cggccgccaa tgtcgaccga gtcaccgatt
     3901 tcgccgccgg cgtcgacgat ctgcgcctcg acgatgccgt gttcgcgggc ctcgcgcccg
     3961 gcgctctggc ggccgacgcc ttccgcatcg gcacccgcgc gatcgacgct tcggaccgga
     4021 tcgtctacaa tcccgaaacc ggcgctttat tcttcgatgc cgacggcacc ggcggggcgg
     4081 cccagatccg cttcgccacc ctcgcccccg gcatagcttt gacggccgcc gacttcctcg
     4141 tcttctgagg gcgcgcgcgc caagggtcca ttctgcgcct tgaccggccg cccccggcgg
     4201 tgcttacggc tgtcggcatg accgacgata tcgcagcgcg ccttgcaggc cagaacatcg
     4261 tgctccccga acccgccgcc cccgtcgccg cctatgtgcc ggcggtgcgg gtcggcaatc
     4321 tgctccacgt ctcgggccag ctcccgttcg atgacggcaa gctgatgacc gggcggctcg
     4381 gcgaggacaa ggacgtcgct tacggctatg ccgccgcccg cgcctgcggg atcatgctgc
     4441 tcgcccagat caaaaaggcg ctgggcgacc tccacaaggt cgagcggatc gtcaagctcg
     4501 gcgtgttcgt caattcggcg gccggcttca ccgaccagcc caaggtggcg aacggcgcct
     4561 ccgagctgat ggaacaggtg ttcggcgagc ccggccgcca tgcccgcagc gcggtcggcg
     4621 tgccgtcgct gccgctcggc gcggcggtcg aagtcgatgc gatcgtcgcc atcaagggca
     4681 gctgaccggg ccggcgagcg gctcacggag ctgattgccc tacccttcgc ccatcgcggc
     4741 ctccacggcg gcggcatcgt cgagaatagc cgcgccgcct tcgccgccgc catcgcccgc
     4801 ggccacggca tcgagctcga cgtccaggcg agccgcgacg gcgatgcctt cgtgttccac
     4861 gaccaaagct gcgaccggct gacgctggcg accggcccga tcgccgcccg cagctccgcc
     4921 gagctcagcc ggctggagct tcgcggcgcc ggcgaaaggc tggccacgct tccggatatt
     4981 ctggcgctga tcgccgcccg cgtgccgctg ctgatcgagg tcaagacggc acgggacgtg
     5041 gcgccgctct gccgctcggt cgccggcgcg ctcgccgatt atcgcggcgc ggccggcgtg
     5101 atgtcgttcg acccgcgggt cggcgcctgg ttcgcccgcc acgcgccgca tcgcctgcgc
     5161 ggcctcgtcg tcaccgaaat gaatcggaaa aacctgcgcg ggcggatgga gcggcgactg
     5221 gcgctggcgc gggcgcggcc ccatttcctc ggttatgaca tacgcgacct cccttccccc
     5281 ttcgccgccg cggctcgcgc gcggggaatt gcggtctgca cttggacggt gcggacggcg
     5341 gcggagggcg cgcgcgcggc cgaacatgcc gaccagatca tctacgagga atgacccaag
     5401 cgaggctggc cgatgagcgg tgagatcctc gcccgtatcg gcgacggcgt cgccagcttc
     5461 gcggccggcg attgggacgg ctgcgccggt gccggcaatc ccttcctcgg ccacgccttc
     5521 ctgtcggcgc tcgaggattc gggcagcgcc agcggccgca ctggctggca gcccgtgccg
     5581 atcgccatcg acggccccga tggccgcccc gccgccgtga tgcccgctta cgccaagagc
     5641 cacagccagg gcgaatatgt gttcgatcat ggctgggcca gcgcctatga gcgcgccggc
     5701 ggccattatt atccgaagct ccagatcgcg gtcccgttca cgcccgtccc cgggcggcgg
     5761 ctgctgaccg ccgagcccgc cttggcgccg gctttgatca aggcggccga ggcggtagtc
     5821 cggagcaacg gcctgtcctc ggcccacgcc accttcatcg atcccgacga agtacggctt
     5881 ttcgagcgcg ccggctggct gatccgcgtc gacagccaat tccactggca caatcgcggc
     5941 tatgcgacct tcgaggattt cctcgccacc ctcgcctcgc gcaagcgtaa ggcgatccgc
     6001 aaggagcgcg ccgcggcgct ggccgggctg accgtggaac atgtcaccgg cggcgccatc
     6061 gaggagcgcc actgggacca tttctggcgc ttctaccagg atacgggcgc gcgcaaatgg
     6121 ggccgcccct atctcaaccg ccgcttcttc accctgctcg gcgagcgcat ggccgaccgc
     6181 gtcctgctga tcttcgcgct ccgcgacgga gtgccgatcg ccggcgcgct caacatgatc
     6241 ggcgcggaca cgctttacgg ccgctattgg ggcgcgaccg aggaggtgcc gcacctccat
     6301 ttcgagatct gctattatca ggcgatcgac gccgccatcg cccgcggcct cgcccgggtc
     6361 gaggccgggg cccagggcga gcacaagctt gcgcggggct acgagccggt gccgacctat
     6421 tcggcccatt atctgctcga cccgagcctg cgcgccgccg tcgccgacta tctcgaaagc
     6481 gagcgccgcg cggtggtcaa cgagatcgat atcctgcacg gcatgacccc gttcaagaag
     6541 ggctagctcc ggcggcggcg aggacgcgct gggcctggac gaggtgcggg cggtcgatca
     6601 tgctcccggc gagcgacagc gcccccgccc ccggctgcgc ctcgaacgcg gcaacgatgg
     6661 cgcgggcatg ctcgacctca gccggcgacg gcgtgaaggc ggcgttgatc accggcacct
     6721 ggtcgggatg gatcgccatc atgccggtaa agccgtcgcg ccgcgcgcgg gcggcataag
     6781 cggccagccc gtcgaggtcg cgaaaggcgg gatagacggt ctcgatcggc gccacgcccg
     6841 ccgccgcggc gccgaacagg cacagcgagc gcgccagctc gtaaggcggc gtgaagcgcc
     6901 cgtccggctc gcgcgaggtc gaagcgccga tcgccgccgg caaatcctcc gccccccagg
     6961 tgaggccggc cagccgcttg gcgccgccat aagtgccgag ctggaacatc gcggccgggg
     7021 tttcggtggc gatggccagg atctgggcgg cgacgttgcc gcgctcggtc agccggcgcg
     7081 ccagctcggc gacggaagcg ccgccctcgg ccttgggcag cacgatgccg tgcgggtggc
     7141 tgccgaggat cgcctgcagg tccctgtcgc attccgggcc gtctagcggg ttgacccgca
     7201 cccacagagg cgccgccgaa ttcgcgccca ggaactcggc caccgcccgc cgcgcctccg
     7261 gcttggccgc cggcgcgacc gcatcctcga gatcgaggat caacgcgtcc gccccggcgc
     7321 ccaaggcctt gtgcatccgg tccggcctgt ccccaggcac gaacagcagc gagcgcagcc
     7381 tcatgtttcc tccccgcaac ggggaggggg accagccgca ggctggtgga gggggccccg
     7441 cctctgcatc agcgcagtcc gctcgcacac gcagaccagc tcaccattct ggttcagcag
     7501 gcggtgctcg aacgtgacga tccccgcctc gggccgtgac ttcgaatcct tgagcgcgat
     7561 cacttccgtc tccgcgcgca gcgtgtcgcc gacgaacacc ggcttcggca tccgcacctt
     7621 gtcgtagccg agattggcga ccagcgtgcc gagcgtcgtg tcccccaccg acaagccgac
     7681 catgagcgcg aacgtgaaag tcccgttgac gacgatccgc ccgaattcgg tcccagcggc
     7741 atattcggcg tcgagatgga gcggctgcgg attgtgggtc agggccgaaa tgaggaggtt
     7801 gtccgtctcg gtgacggtcc ggtgcggctg atgctgcagc cggtcgccga ccgtccattc
     7861 gtcataatag cgcccggtca tcttaattcc tccccggcac ggggaggggg accgcgccgc
     7921 gaagcggcgg ggtggagggg ggctcgcgcc tggcacgccg ccccctccac cgcacttcgt
     7981 gcggtccccc tccccgtgcc ggggaggaat taagatgacc gggcgctatt atgacgaatg
     8041 gacggtcggc gacc
//
LOCUS       DASVRK010000214         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_232000, whole genome shotgun sequence.
ACCESSION   DASVRK010000214 DASVRK010000000
VERSION     DASVRK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232000"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..777)
                     /locus_tag="VF589_08465"
     CDS             complement(67..777)
                     /locus_tag="VF589_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257655.1"
                     /translation="MDDRRGGPGDIPTAERMRDVGTAMASRMGQAVTGNRARDQSGAE
                     ARLTEADVAALTGDWPRGPRNIIEQMLRQYGPPNEGTRYRLIWYYNGPWKRTEVTRDE
                     IAHNFPAPHTDYITNWIDYPVPVDLATELTRYDGSCLIDRTAGEAGARCDSEAANFIT
                     LNFMHEIATGRKSVAAAREAYASQMAAYLVGRPAPYAERLLFDPPAGGTVDPDEAVLA
                     PQVEQVKEKVKDLFRGDG"
     gene            1159..>5075
                     /locus_tag="VF589_08470"
     CDS             1159..>5075
                     /locus_tag="VF589_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257656.1"
                     /translation="MTFAATLSVNSLNGANGFTLSGPTDLGSGYSVDSAGDVNGDGFD
                     DLIIGTFGQYGPPGYSYVVFGRGTALPDVIDLAALDGTNGFRLSQEESFSGTSVSSAG
                     DLNGDGYGDLLVGTNGTDDEGPNSAGDSYVVYGKAGGFAADLQLGALNSFNGFRLDAL
                     DLGFDSSGSYVSSAGDVNNDGLDDILVSVTGTTGSDTGSVGYVVFGSQTPLPVNFSLA
                     SLDGVQGFRIDNMGEARAVSSAGDINNDGIDDFVIGDSSADGGSGRSYVVFGRNGSFG
                     PSIDLAALNGSDGFALTGAAEGDYSGTSIASAGDVNGDGYDDLIVGANGAGAYDGAAY
                     VVFGKPGFEAELDLGALSDADGFTILGTEYSAAGYAVSGAGDVNGDGYDDLLVAAPSI
                     TGTTGSAYVVFGNKDGLFGGLANLDGSNGFRISDIPVGENGGIAVAAAGDVNGDLLDD
                     IIVGSSAAGESYVIYGRIPTGDPTLRVGSDADQTIHGSNTVAGFDYLYGEGGDDIIIP
                     YYGSDQARGGDGIDILYVDYSQLGSVSFNTNIVTSPSGSVTFYRIRGLAPAGGESSNV
                     EYRDFERFFVIGSATANNDLRGGSGGDYLVGGLGSDTIYTFGEPQLDPGPANSGDDIV
                     DVGKSGATAATSGTDNVQMGSGGDDLLIVDWSAATDAVNFAAGSGNSQDYFSSGSGVF
                     LGSSVRLNFTGVERFVVTTGTGNDAITTGDNADTVSAGAGNDAINSRKGRDTIDGGSD
                     IDSWSADFTPQTSAVILDLNDAGTTTLAGGTTVRNIESLLNFNTGSGHDVLTTFASGA
                     PGDTIDTGGGNDRVTLFSAATAATTGTDNVGLGTGGNDLLVVNWSAATEAVNFAAGSG
                     TTQDYFSAGSGVFLGTSVRLHFTGVERFAVTTGSNNDTITTGDNADSIFSGGGNDTLD
                     SRAGAAVINGGAGIDRWAADLSASGAAILLNLNLAGAQALAGGSSITAIEALTLTTGT
                     ANDVITTRAAGTEGDTLTTGAGDDTATLYSAATAALTGTDNTHMGTGGNDLLVVDWSA
                     ATEAVGFAAGSANTQDYFSSGSGVFLGSSVRLNFTGVERFAVTTGSGNDAITTGDNND
                     SIGSGGGNDTLDSRAGIATINGGAGTDRWAADLSAFGGAIILNLNMAGAQALADGSSI
                     TAIEALTLTTGAGNDVITTRSAGSEGDILNTGLGDDTATLFSAGTAVTTGTDNVGLGT
                     GGNDLLVVDWSAATEAVNFAAGSGTTQDYFSAGSGVFLGSSVRLHFTGVERFAVTTGS
                     ANDTITTGDNADSISSGGGNDTLDSRAGTATINGGAGTDRWAA"
BASE COUNT          836 a         1844 c         1582 g          813 t
ORIGIN      
        1 cccgcaagat tccagcttgc gctggaatga cgggccgggc ccggcccgcc tcagcccacg
       61 ctcgcctcag ccgtcgccgc ggaacaggtc cttcaccttc tccttcactt gctcaacctg
      121 cggcgcgagt accgcctcgt cggggtcgac cgtgccgccc gccggcgggt cgaagagcag
      181 ccgctcggcg taaggcgcgg gacggccgac cagataggcc gccatttgcg aggcataggc
      241 ttcgcgcgcc gctgccacgc ttttccggcc ggtcgcgatc tcgtgcatga agttgagggt
      301 gatgaagttg gccgcctcgc tgtcgcagcg ggcgcccgct tcgccggcgg tgcggtcgat
      361 caggcagctg ccgtcgtagc gcgtgagctc ggtggcgagg tcgaccggaa ccggatagtc
      421 gatccagttg gtgatatagt cggtgtgcgg ggccgggaaa ttgtgggcga tctcgtcgcg
      481 ggtcacctcg gtccgcttcc aggggccgtt ataataccaa atgaggcggt agcgcgtgcc
      541 ttcgttgggc ggcccatatt ggcgcagcat ctgttcgatg atgttgcgcg ggcccctggg
      601 ccagtcgccc gtcagcgccg cgacgtcggc ttcggtcagc cgggcctcgg cgccggattg
      661 gtcgcgggcc cggtttccgg tcaccgcctg gcccatgcgg ctggccatcg cggtgccgac
      721 atcccgcatc cgctccgccg tggggatgtc gccggggccg ccgcgtcgat cgtccatctc
      781 attgtccttt ttcgacgtct cccctgccga acgcctcccc ggcggtaagg ttcgctcgac
      841 tcgccgtcat tccagcgaaa gctggaatct cccttcccct tcccgacccg caagattcca
      901 gcttgcgctg gaatgacgag ggggcgctgg aatcacgacg ggggcgctgg aatgacgagg
      961 ggggcgactt catagtcccc ccaccttctt aacctatgtt ccccgcccag aaatggctcg
     1021 acaatttgac catttaggca gatacggcgc ttctagtggg gaatcagcag tttaggccca
     1081 gtgctttgga tggcggcccg gatcagtctc cccaatggtc gccgtccggc tgaggacgtg
     1141 cgcgcggggg ggccgacaat gacgttcgca gctacgcttt ccgtaaactc gctcaacggc
     1201 gccaatggct tcaccctcag cggccccacc gacttgggga gcggctactc ggtcgactcc
     1261 gccggcgacg tcaatggcga cggcttcgac gacctcatca tcggcacatt cggtcaatac
     1321 ggcccgcccg gctacagcta tgtcgtgttc ggccgaggga cggccctccc cgacgtgatc
     1381 gaccttgccg cattggacgg caccaacggt ttccgcctca gccaggaaga aagcttttcc
     1441 ggaacctcgg tttcctcggc cggcgacctc aatggcgacg gctatggcga cctgctggtc
     1501 ggcaccaacg gcaccgacga cgagggcccc aattccgccg gcgattccta tgtcgtctac
     1561 ggcaaagcgg gcggcttcgc cgccgacctc cagctcggcg ccttgaacag cttcaacggc
     1621 ttccggctcg acgccctcga tctcggcttc gactccagcg gcagttatgt atcgtcggcc
     1681 ggcgacgtca acaatgacgg cctcgacgac atcctcgtat cggtgaccgg caccaccggc
     1741 tccgacactg gaagcgtcgg ctatgtggtg ttcggcagcc agacgccgct gccggtgaat
     1801 ttcagcctcg cctcgctcga cggcgtgcag ggctttcgca tcgacaatat gggcgaggcc
     1861 cgcgcggtct cgagcgcggg cgatatcaac aatgacggca tcgacgattt cgtcatcggc
     1921 gacagctccg ccgacggcgg cagcggccgc tcctatgtgg tgttcggccg caacggcagc
     1981 ttcgggccga gcatcgatct ggccgcgctc aacggcagcg acggcttcgc gctgaccggc
     2041 gccgctgaag gcgattatag cggcacctcc atcgcctcgg cgggcgacgt caatggcgac
     2101 ggctatgacg atctgatcgt cggcgcgaat ggagccggcg cttatgacgg cgcggcctat
     2161 gtcgtgttcg gcaagcccgg tttcgaggcg gagctcgatc ttggcgcgct cagcgacgcc
     2221 gacggcttca ccatcctcgg caccgaatat tccgccgccg gctatgcggt gtccggagcc
     2281 ggcgacgtca acggcgacgg ctatgacgat ttgctcgttg ccgctcccag cattaccggg
     2341 acgaccggct ccgcttatgt ggtgttcggc aacaaggacg gcctattcgg cgggctcgcg
     2401 aacctcgacg gcagcaacgg cttccgcatc tccgacatac cggtcggcga gaatggcggt
     2461 atcgccgtcg ccgccgcggg cgacgtcaac ggcgaccttc tcgacgacat catcgtcggc
     2521 tcgtcagccg ccggcgagag ctatgtgatc tacgggcgga tcccgaccgg cgatccgacg
     2581 ctgcgggtcg gctccgacgc cgaccagacc atccacggca gcaacaccgt cgccggcttc
     2641 gactatctgt acggcgaagg cggggacgac atcatcatcc cttattacgg cagcgaccag
     2701 gcgcgcggcg gcgacggcat cgacatcctg tacgtcgact atagccaatt gggctcggtc
     2761 tccttcaaca ccaatatcgt caccagcccg agcggcagcg tgaccttcta ccgcatccgc
     2821 gggctggcgc ccgccggcgg tgaatcctcg aacgtggaat atcgcgattt cgagcgcttc
     2881 ttcgtgatcg gcagcgcgac cgccaacaac gatctgcgcg gcggctcggg cggcgattat
     2941 ctcgtcggag ggctcggcag cgacactatc tacaccttcg gcgagcctca gctggacccc
     3001 ggccccgcca attcgggcga cgatatcgtc gacgtgggca aaagcggcgc gaccgccgcc
     3061 acctccggca ccgacaacgt ccagatgggc agcggcggcg acgaccttct gatcgtcgac
     3121 tggtcggccg ccaccgacgc ggtcaatttc gccgctggct cgggcaacag ccaggattat
     3181 ttctcctcgg gctcgggcgt cttcctcggc agttcggtgc ggctcaactt caccggcgtc
     3241 gagcgcttcg tggtcaccac cggcaccggc aacgacgcta tcaccaccgg cgacaatgcc
     3301 gacaccgtga gcgccggcgc gggcaacgac gcgatcaaca gccgcaaggg ccgcgacacg
     3361 atcgacggcg gcagcgacat cgacagctgg tccgcggatt tcacacccca gacgagcgcc
     3421 gtgatcctcg acctcaatga cgctggcacg accaccctgg ccgggggaac gaccgtccgg
     3481 aatatcgaga gcctgctgaa cttcaacacc gggtccggcc acgacgtcct caccaccttc
     3541 gccagcggcg ctccggggga cacgatcgac accggcggcg gcaacgaccg cgtcaccctg
     3601 ttcagcgccg ccaccgccgc caccaccggc accgacaatg tcggcctcgg caccggcggc
     3661 aacgacctgc tggtcgtcaa ctggtcggcc gccaccgaag cggtcaactt cgcggcggga
     3721 tcgggcacca cccaggatta tttctcggcc ggctccggcg tcttcctcgg cacctcggtc
     3781 cggctccact tcaccggcgt cgagcgcttc gcggtcacca ccggctccaa caacgacacc
     3841 atcaccaccg gcgacaatgc cgacagcatc ttctcgggcg gcggcaacga cacgctcgac
     3901 agccgcgccg gcgcggcggt catcaacggc ggcgccggca tcgaccgctg ggccgccgac
     3961 ctctccgcct ccggcgccgc gatcctgctc aacctcaacc tggccggagc ccaggcgctg
     4021 gccggcggca gcagcatcac cgccatcgaa gcgctcaccc tcaccaccgg caccgccaac
     4081 gacgtcatca ccacccgcgc cgctggcacc gaaggcgaca cgctcaccac cggtgccggc
     4141 gacgacaccg ccaccctgta cagcgccgcc accgccgccc tcaccggcac cgacaatacc
     4201 catatgggca ccggcggcaa cgacctgctc gtcgtcgact ggtcggccgc caccgaggcg
     4261 gtcggcttcg cggccgggtc cgccaacacc caggattatt tctcgtcggg ctctggcgtc
     4321 ttcctcggca gctcggtccg gctcaacttc actggcgtcg agcgcttcgc ggtcaccacc
     4381 ggttcgggca acgacgcgat caccaccggc gacaacaatg acagcatcgg gtcgggcggc
     4441 ggcaacgaca cgctcgacag ccgcgccggc atcgcaacca tcaacggcgg cgccggcacc
     4501 gaccgctggg ccgccgattt gtcggcgttc ggcggagcca tcattctgaa cctcaatatg
     4561 gccggagccc aggcgctcgc cgacggcagc agcatcacgg cgatcgaggc gctgaccctc
     4621 accaccgggg ccggcaacga cgtcatcacc acccgctccg ccggcagcga gggcgacatc
     4681 ctcaacaccg ggctcggcga cgacaccgcc accctgttca gcgccggcac cgccgtcacc
     4741 accggtaccg acaatgtcgg gctcggcacc ggcggcaacg acctgctcgt ggtcgactgg
     4801 tccgccgcca ccgaagcggt caatttcgcg gcgggatcgg gcacgaccca ggattatttc
     4861 tcggcgggct cgggcgtgtt cctcggcagc tcggtgcggc tccacttcac gggcgtcgag
     4921 cggttcgccg tcaccaccgg ctcggccaac gacaccatca ccaccggcga caatgccgac
     4981 agcatctcgt ctggcggcgg caacgacacg ctcgacagcc gcgccggcac cgcaaccatc
     5041 aacggcggcg ccggcaccga ccgctgggcc gccga
//
LOCUS       DASVRK010000215         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_40497, whole genome shotgun sequence.
ACCESSION   DASVRK010000215 DASVRK010000000
VERSION     DASVRK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40497"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VF589_08475"
     CDS             complement(<1..374)
                     /locus_tag="VF589_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX8257657.1"
                     /translation="MFRAGAEACLPGRLLPRHLPEPPPGRTILLAVGKAAIGMAAAAE
                     REWTGELAGLAVAPYGSQGSLRRIETMTAAHPVPDGASLAAGERLLALAGEAGADDLV
                     LVLLSGGASALAAAPAPGLTLEA"
     gene            464..1156
                     /locus_tag="VF589_08480"
     CDS             464..1156
                     /locus_tag="VF589_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HEX8257658.1"
                     /translation="MPLALAARRTLKAFGIAVLALLAFFVLYFAAALVGALIPANSDW
                     REPKDGVRIFLRTNGVHTWIMVPTVRPEMDWRPWVPAAHIADPRYAGNYLAFGYGNRD
                     FYLNTPTWWDLRPRTALAAAFGGGPALVHAEYEHDPRRDEWHRPLTISPEEYRRLAAF
                     IRASFKTGADGRTRPVLGRGYGRADIFYAAGGRYDMFRTCNEWTGEALRAAGIRTGIW
                     TPLERSIMWRLE"
     gene            complement(1163..1627)
                     /locus_tag="VF589_08485"
     CDS             complement(1163..1627)
                     /locus_tag="VF589_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257659.1"
                     /translation="MADGGAGLLASAAPVSPGASSGRDREVSLLRLHLLRANYLLWAV
                     AGLFFALPPLLDPDPAARGMIDSMFGGLWAMGVLGVRYPLQMLPIFLFEFVWKTIWLL
                     VFGLPQWLAGDGSERLGEDLIGIGNGPILFGLIIPWGYVWRHYVREPAERWR"
     gene            complement(1620..2315)
                     /locus_tag="VF589_08490"
     CDS             complement(1620..2315)
                     /locus_tag="VF589_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257660.1"
                     /translation="MNLFLWAHIAGGSLALLAGAAAVAARKGGALHARAGTWFAISML
                     VLGVTATILAQADDDTGLGLGGIITCYFVATSWMTARRRDGTSGRFEVAAGALALASA
                     AAITWSAWAGDASTPAGAGPLFGLAALCLLGGIGDLRAVVAGKLTPVQRLSRHLWRMC
                     FAFFIATGSFFLGQQDVMPKEVRGAPILFVLALAPFAVMLFWLVRLRFAKAIARSKRT
                     GLMFIGRRAQPDG"
     gene            complement(2312..2782)
                     /locus_tag="VF589_08495"
     CDS             complement(2312..2782)
                     /locus_tag="VF589_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257661.1"
                     /translation="MSNGTIDERTAAPDHGVSNARLIALRALYALIVVGLAFFVWPSF
                     LAQLPAPAHFHGVAMTMLAAFSILCAVGIRYPLQMLPILFWELLWKGLWLLLIALPRW
                     MAGTMDAATTQTAIDCAAVVLVLAAVPWGYVARHYVSKPAERALRPAAAAAGAA"
     gene            complement(2796..3059)
                     /locus_tag="VF589_08500"
     CDS             complement(2796..3059)
                     /locus_tag="VF589_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8257662.1"
                     /translation="MAIAVKLDDVLYARRMTLTELAERVGITIVNLSILKTGKARAIR
                     FSTLEAICRELQCQPGDILEFTADQGSGIDGADDQDTGSRAAA"
     gene            complement(3062..3583)
                     /locus_tag="VF589_08505"
     CDS             complement(3062..3583)
                     /locus_tag="VF589_08505"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX8257663.1"
                     /translation="MPRSYSAALPIAFVVLRIMIVLNWLLGAAILVLLFLMPNERWII
                     SAFELSPSPEARRVVMGLRAVAAVGLVTIPLNYIVLKRLLAMVGSVRAGNPFVAANAQ
                     RLQAIAWALLALQLLSLVIGGIAKAVSSPGHPVDLDAGFSVNGWLAVLLTFVLARVFA
                     EGTLMREDLEGTV"
     gene            complement(3764..>4096)
                     /locus_tag="VF589_08510"
     CDS             complement(3764..>4096)
                     /locus_tag="VF589_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8257664.1"
                     /translation="EQGVIQRRVALVDPEAIGLGLTVFVEIEAGDHSAEWLARFAAAV
                     TQMPEVMEVYRMAGDVDYLLRIAVPNMGAYDDFYRRLIGEVPIKNVTSRFAMERVKAT
                     TAYPIAIP"
BASE COUNT          715 a         1479 c         1338 g          564 t
ORIGIN      
        1 gcctccaggg tcagcccggg cgccggcgcc gccgccagcg ccgaagcgcc gccgctcagc
       61 agcaccagca cgaggtcgtc cgcccccgct tccccggcca gcgccagcag ccgctcgccg
      121 gccgcgaggc tggcgccgtc cggcaccgga tgcgccgccg tcatcgtctc gatccgccgc
      181 agcgatcctt gggagccata aggagccacc gctagccccg ccagctcgcc cgtccactcc
      241 cgctcggccg ctgccgccat cccgatcgcc gccttgccca ccgccagcag gatcgtgcgc
      301 cccggcggcg gctccggcag gtgccggggc agcagccggc cgggcaggca cgcctcggcg
      361 ccagcgcgaa acatcgtctc caaatcccgg cgcgcgctca ccggccgagc gtgcccgcac
      421 aagcccgctt catcaacccg cgcgatgcgg ctaagctggg gcgatgccgc ttgccctcgc
      481 cgcccgacgc acgctcaagg ctttcggcat cgccgtcctg gcgctgcttg catttttcgt
      541 cctctatttc gcggccgccc tggtgggggc gctgattccg gccaactcgg actggcgcga
      601 gccgaaggac ggcgtccgca tcttcctgcg caccaacggc gtccatacct ggatcatggt
      661 gccgaccgtg cggccggaga tggactggcg cccgtgggtg cccgccgccc acatcgccga
      721 tccgcgctac gccggaaact atctcgcctt cggctacggc aatcgcgatt tctacctcaa
      781 caccccgacc tggtgggacc tgcgcccgcg cactgctttg gcggccgcgt tcggcggcgg
      841 gccggctctg gtccatgccg aatatgagca tgatccgcgc cgcgacgaat ggcaccgccc
      901 gctcaccatc agccccgagg aatatcgccg gctcgccgcc ttcatccgcg ccagcttcaa
      961 gaccggtgcc gatggccgca cccgccccgt gctcggccgc ggctacggcc gggcggacat
     1021 cttctacgcg gccggcggcc gctatgacat gttccgtacc tgcaacgaat ggaccggcga
     1081 ggcgctccgc gccgccggca tccgcaccgg catctggacc ccgctcgagc gcagcatcat
     1141 gtggcggctc gaatgattgg cctcaacgcc agcgctccgc cggctcccgc acatagtggc
     1201 gccagacata gccccagggg atgatcagcc cgaacaggat cgggccgttg ccgatcccga
     1261 tcagatcctc acccagccgc tccgagccgt cgcccgccag ccattgcggc aggccgaaca
     1321 cgagcagcca gatcgtcttc cacacgaatt cgaacaggaa gattggaagc atctgcagcg
     1381 ggtagcgcac gcccaacacg cccatcgccc ataagccgcc gaacatgctg tcgatcattc
     1441 cgcgcgccgc cggatcggga tcgagcagcg gcggcagcgc gaaaaacaaa ccggcgacgg
     1501 cccacagcag ataattggcg cggagcaggt gcagccgcag cagcgagact tcgcgatcgc
     1561 gcccgctact cgcgccaggc gaaacgggag cggcggaagc aagcaggccc gcccctccgt
     1621 cagccatctg gctgggcccg ccgcccgatg aacatcagcc cggtccgctt cgaccgcgcg
     1681 atcgccttgg cgaagcgcag gcgcaccagc cagaacagca tcaccgcgaa cggcgcgagc
     1741 gcgaggacga acaggatggg cgcgccgcgc acctccttcg gcatcacgtc ctgctggccc
     1801 aggaagaagg atcccgtggc gatgaagaaa gcgaagcaca tccgccagag gtgacgcgaa
     1861 aggcgctgga ccggcgtcag cttgcccgcg acgaccgccc tcaagtcgcc gatcccgcca
     1921 agcaggcaca aggcggccag gccgaataac ggtccggcgc cggccggcgt ggacgcatct
     1981 ccggcccagg ctgaccaggt gatggcggcg gcggaagcca gcgccagcgc gccggcggcg
     2041 acctcgaacc ggccgctggt cccgtcgcgg cgccgcgccg tcatccagga ggtggcgacg
     2101 aaatagcagg tgatgatgcc gcccaggccc agcccggtat cgtcatcggc ctgcgcgagg
     2161 atcgtggccg tcacgcccag caccagcatt gagatggcga accaggttcc ggcgcgggcg
     2221 tggagcgctc cgcccttgcg cgccgcaacc gccgccgcgc cggcgaggag ggcgaggctt
     2281 ccgcccgcga tgtgcgccca caggaagagg ttcatgccgc gcccgccgcg gcggcggccg
     2341 gacgcaacgc ccgctcggca ggcttcgaca cataatggcg cgcgacatag ccccagggca
     2401 ccgccgccag caccagcacc accgcggcgc agtcgatcgc cgtctgggtg gtggccgcgt
     2461 ccatcgtgcc ggccatccag cgcggcaggg cgatcagcag cagccacagg cctttccaga
     2521 gcagctccca gaaaaggatc ggcagcatct gcagcggata gcggatgccg accgcgcaca
     2581 gaattgagaa ggccgcgagc atggtcatcg cgacgccgtg aaagtgcgcc ggcgccggga
     2641 gctgggcgag gaaggagggc cagacgaaga aggccagccc tacgacgatg agcgcgtaga
     2701 gcgcgcgcaa ggcgatcagg cgcgcgttcg acactccgtg atcgggggcc gctgtgcgct
     2761 cgtcgatggt gccgttgctc atggtctgct ccttctcagg cggccgctcg cgacccggtg
     2821 tcttgatcgt ccgcgccgtc gatgccgctg ccttggtcgg cggtgaattc gaggatgtcg
     2881 cccggctggc attggagctc gcggcagatc gcctcgagcg tcgagaaacg gatcgcgcgc
     2941 gccttgccgg tcttcaggat cgacaggttg acgatggtga tgccgacccg ctcggcgagc
     3001 tcggtgagcg tcatgcgccg cgcgtagagc acgtcgtcca gcttgaccgc gatggccatc
     3061 gtcagaccgt cccctcgagg tcttcgcgca tcagcgtgcc ttcggcgaac acccgcgcca
     3121 gcacgaaggt gagcagcacg gccagccagc cgttgacgga gaagcccgcg tcgagatcga
     3181 ccgggtgccc cgggctcgaa accgctttgg cgatcccccc gatgacgagg ctgagcagct
     3241 gcagcgcgag cagggcccag gcgatcgcct gcaatctttg cgcattggcg gcgacgaacg
     3301 ggttgccggc gcggaccgat ccgaccatcg cgagcagccg cttgaggacg atataattga
     3361 gcgggatcgt gaccaggccg accgccgcca ccgcgcgcag ccccatcacc acccgcctcg
     3421 cctcgggcga gggcgaaagc tcgaacgcgc ttatgatcca gcgctcgttc ggcatcagga
     3481 acagcaggac gagaatggcc gcgccgagca gccagttgag cacgatcatg atccgcagca
     3541 ctacgaaggc gatcggcaag gcggcggaat aggatcgggg catcggcttt ggctccagcg
     3601 gctcttaata tcggtaaacg ataaagcatt tatcgaatgt cgtcaaacat tcccgtcatt
     3661 ccagcgcaag ctggaaactt gcggccggga agaagaaggg agataccagc tttcgctgga
     3721 atgacgggag gagattccag ccttctgccc cgtcagcgcc tcgtcagggt atcgctatcg
     3781 gataggcggt ggtcgccttc acccgctcca tcgcgaagcg cgaggtgacg ttcttgatcg
     3841 gcacctcgcc gatcagccgc cggtagaaat cgtcataggc gcccatgttc ggcaccgcga
     3901 tgcggagcag atagtcgacg tcgccggcca tgcgatagac ttccatcact tccggcatct
     3961 gcgtcaccgc cgcggcgaag cgcgcgagcc attcggccga atggtcgccg gcctcgatct
     4021 cgacgaacac ggtgaggccg agcccgatcg cctcgggatc gaccagcgcc acccggcgct
     4081 ggatcacgcc ttgctc
//
LOCUS       DASVRK010000216         4413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_105898, whole genome shotgun sequence.
ACCESSION   DASVRK010000216 DASVRK010000000
VERSION     DASVRK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105898"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..480
                     /locus_tag="VF589_08515"
     CDS             181..480
                     /locus_tag="VF589_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257665.1"
                     /translation="MASNNPSFPIRTAVVSWFDPGGQLHLRVYSSDGYNVTERCNDGE
                     GWVSGFSMPGSDISATCWQDSEGVHLRVYCTFQDKTVEWCYDQNSDWYQGAYTTT"
     gene            610..2289
                     /locus_tag="VF589_08520"
     CDS             610..2289
                     /locus_tag="VF589_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX8257666.1"
                     /translation="MKFPLKELLIILYLAAGYGAVGDRIGSLGLSLPLLLYLGLFGFL
                     TACLLLAAYVRNNALRWGYAALLSASALFLDSSERILGDHLSYDAFINLLNSAGFAGD
                     ALGQHRAPIAAAAFGALLLFAGIALKPRRSPPLAFAAAVAPIAGIAFLAALLFVRGGE
                     GARGLPGAFTALSYSSLSAYEGAGWGGERRPVALPRAAGPAGRDIVLIVDESVAANYL
                     DLGHPDGVRSGLNEPRAGLAIRNYGYAAAITNCSVGTNLTLRFGGTRSDYQRINANMP
                     SVWDYARKAGLGTVYIDAQRTGGQLHNLMTAEELAGVDRHIQFDGVPVQRRDMAAADA
                     LAGLLNNGTREFILVNKVGAHFPIHDKYPDAFMRYRPALPRGGHEDVSDTGSRAGFGG
                     TAEDWRRYRNAYRNTLLWNVGGFFDRLLARTDLSRATLIYTSDHGQDLHERGRPGLNT
                     HCSSEPVIEEGLVPLVVIEGAGASALNWDRHLAANRNRVSHYQLFPTLLALMGYDEAP
                     VRAVYGPSLVERSTDALTFNTLFNARLGREPEWKRIELAKVTAPPPSDRGP"
     gene            complement(2310..2687)
                     /locus_tag="VF589_08525"
     CDS             complement(2310..2687)
                     /locus_tag="VF589_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX8257667.1"
                     /translation="MTDAKREPILRLVPRPADINANGHIFGGWVLSQMDIAAGIVASR
                     RAEGPVATVAIEAMEFIAPILLHDIISVYAHVERTGRTSMGLRIEVVASRDRGKQEVK
                     VTEALFTFVALDESNRPKPLPAQ"
     gene            complement(2674..3057)
                     /locus_tag="VF589_08530"
     CDS             complement(2674..3057)
                     /locus_tag="VF589_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257668.1"
                     /translation="MPRHDPIPPRAERTAIAWARYKKLMGWMVVAAVVAVLLSLVYLK
                     SSGGPVPIHMIIATVAGVGFSVLLGTALMGLVYFSNNSGHDEGTHRGGGTAMGSGDED
                     VHRSGTSMGFPSEGAARHKDHDDRR"
     gene            3132..3560
                     /locus_tag="VF589_08535"
     CDS             3132..3560
                     /locus_tag="VF589_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8257669.1"
                     /translation="MTIGAILGGKGDEVVSVGCETIVRDAVARLADKRIGAMPVLREG
                     EVVGIISERDIINCINTDGAAMLDWKVDRVMTTPPITVTREVSVLAALSMMTKRRIRH
                     LPVLEGTSVIGIVSIGDLVKYRIDRIEQEAEAMRAYIQSA"
     gene            complement(3557..>4413)
                     /locus_tag="VF589_08540"
     CDS             complement(3557..>4413)
                     /locus_tag="VF589_08540"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8257670.1"
                     /translation="FAGAGRLFSYGGTMVAIQFFWFLQSQSDVFIAGRLLDPHRLGLY
                     TTALFLTQILAAKFVPPLNEVAFAAYSRMQADRDMMAGAFVKAARLIMLVTLPFYFGL
                     AVTVEPLVLTFLGPKWAGIVAIVPTLALAMPFLTLQILFAPATNALGRPGIALATGVV
                     GAAILPTAFLIGIGHGLAGLAYAWLGGMALLTLATVALSAPAIGLNLGELLRALRPGF
                     LASAAMAAAVLALDGALPPMPPQPRLAILVAAGAALYGGLLLAFARPLVVELVALIRG
                     RRGEPAAA"
BASE COUNT          739 a         1543 c         1454 g          677 t
ORIGIN      
        1 ctcggctatg tgatggaata tatcccggag ccggaggagg agcaggagga ggaagactga
       61 cgggtgccgg tcgcgccgct gtggattcgt ccccacttgc aagtgtcgag gcaaaggatg
      121 agtgttcctc cagcgcaacg attcgttcgt tcgcctgcca cgcagatagg ggagggaaat
      181 atggcttcca acaacccgag ctttccgatc aggaccgcgg tcgtcagctg gttcgatccg
      241 ggagggcagc tccatctccg ggtctattcg agcgacgggt acaatgtgac cgaaagatgc
      301 aacgacggcg agggctgggt gtccggcttc agcatgcctg gatcggacat ctcggcaacc
      361 tgctggcagg attcggaagg cgtccacctg cgtgtctatt gcaccttcca ggacaagacc
      421 gtcgaatggt gctacgatca aaattccgac tggtaccagg gcgcctacac caccacctga
      481 ggcaaggctc gccccggtcg atgaagggtc cggccgcggg gccggacgcg ccctcgctcc
      541 gcccgcttaa ccaagcctta tccgagcgcc gctaagcgtc ggacggtctg gggaagcgga
      601 agggcgccgg tgaagttccc gctcaaggag ctgctgatca tcttgtacct cgccgccgga
      661 tatggggcgg tcggggacag gatcggctcg ctcggcctgt ccctgccgct gctgctctat
      721 ctcggcctgt tcggcttcct caccgcctgc ctgctgctcg cggcctatgt ccgcaacaac
      781 gcgctgcgct ggggctatgc cgccctgctc tcggcctcgg ccctgtttct tgattcgtcc
      841 gaacggatcc tcggcgatca tctcagctac gacgccttca tcaacctcct gaactcggcc
      901 gggttcgccg gcgacgcgct cggccagcac agggcgccga tcgctgcggc ggcgttcggc
      961 gccctgctgc tgttcgccgg catcgcgctg aagccgcggc ggtcgccgcc gctggccttc
     1021 gccgccgccg tagcgccgat cgccggcatc gccttcctcg ccgcgctgct gttcgtgcgc
     1081 ggcggcgagg gcgcccgcgg cctcccggga gccttcaccg cgctcagcta ttcgagcctg
     1141 tcggcctacg aaggcgccgg ctggggcggc gaacgccggc cggtggcgct gccccgcgcc
     1201 gccggcccgg ccgggcgcga catcgtcctc atcgtcgatg agagcgtcgc cgccaattat
     1261 ctcgacctcg gccacccgga cggcgtcagg tccgggctca acgagcccag ggccggcctc
     1321 gcgatccgca attacggata tgccgccgcg atcaccaatt gcagcgtcgg caccaacctc
     1381 accctgcgct tcggcggcac ccggtccgat taccagcgca tcaacgccaa tatgccctcg
     1441 gtgtgggatt atgcgcgcaa ggccgggctc ggcaccgtct atatcgacgc ccagcggacc
     1501 ggcgggcagc tccacaatct gatgacggcg gaagagctcg ccggcgtcga ccggcacatc
     1561 caatttgacg gcgtgcctgt gcagcgccgc gacatggccg cggccgatgc gctggccggg
     1621 ctgctcaaca acggcacccg cgagttcatc ctcgtcaaca aggtcggcgc gcatttcccg
     1681 atccacgaca aatatccgga cgccttcatg cgctaccggc ccgccctgcc gcgcggcggc
     1741 cacgaggatg tcagcgacac cggcagccgc gccggcttcg gcggcaccgc cgaggattgg
     1801 cggcgctacc gcaacgccta tcgcaacact ttgctgtgga acgtcggcgg cttcttcgac
     1861 cggctgctgg cgcgcaccga tctttcgcgg gcgacgctca tctacacgtc cgaccatggc
     1921 caggatctgc acgagcgcgg ccggcctggg ctcaacaccc attgcagcag cgagccggtg
     1981 atcgaggagg ggctggtgcc gctggtggtg atcgagggcg ccggcgcctc ggcgctcaac
     2041 tgggatcgcc acctcgccgc caatcgcaac cgggtcagcc actaccaatt gttccccacc
     2101 ttgctggcgc tgatgggcta tgacgaggcg ccggtccggg cggtctacgg cccctcgctg
     2161 gtcgaaaggt ccaccgacgc gctgaccttc aataccctgt tcaacgcgcg gctcggtcgc
     2221 gagcctgagt ggaagcggat cgagcttgcc aaggtcacgg cgccgccgcc gtccgaccga
     2281 ggcccctgac ggactgtccg atccggcagc tactgcgccg gcagcggctt cggccggttg
     2341 ctctcgtcca atgcgacgaa ggtgaacagc gcctcggtca ccttcacttc ctgcttgccg
     2401 cggtcgcggc tcgcgacgac ctcgatgcga agccccatcg aggtgcgccc ggtccgctcg
     2461 acatgcgcgt agaccgagat gatgtcgtgg aggaggatcg gcgcgatgaa ctccatcgcc
     2521 tcgatcgcga cggtggcgac cggcccctcg gcgcgccggg acgcgacgat gccggcggcg
     2581 atgtccatct ggctcagcac ccagccgccg aaaatgtggc cgttggcgtt gatgtcggct
     2641 gggcgaggga cgagccgcag gatcggctcg cgcttagcgt cggtcatcgt gatccttatg
     2701 gcgggcggcg ccctcgcttg ggaagcccat cgacgtaccg cttcgatgaa catcctcgtc
     2761 gcccgagccc atcgcggtgc cgccgccgcg atgggtgccc tcgtcatggc cgctattgtt
     2821 gctgaaatag acgaggccca tcaaagccgt gccgagcagc accgaaaatc cgactccggc
     2881 gacggtcgcg atgatcatat ggatcgggac cggcccgccc gagcttttga gatagacgag
     2941 cgagagcagc accgcgacca ccgccgccac caccatccag cccatcagct tcttgtagcg
     3001 cgcccaggcg atggcggtgc gctccgcccg cggcggaatc gggtcgtgtc tgggcatgct
     3061 tccctttgcg ggacggtcat gtgatcatca aggcctgagt cggcgtgaaa acgagaaacg
     3121 gaggccggga catgacgata ggcgcgattc tgggcgggaa gggcgatgaa gtcgtcagcg
     3181 tcgggtgcga gacgatcgtg cgcgacgcgg tcgcgcgcct ggccgacaag cgaatcggcg
     3241 ccatgcccgt gctccgcgag ggcgaggtgg tcggcatcat ttccgagcgc gacatcatca
     3301 actgcatcaa taccgacggc gcggcgatgc tcgactggaa ggtcgaccgg gtgatgacga
     3361 cgccgccgat caccgtcacg cgcgaggttt cggtgctggc cgccttgtcg atgatgacca
     3421 agcgccggat ccgacacctg ccggtgctcg agggcacaag cgtgatcggc atcgtctcga
     3481 tcggcgacct cgtcaaatac cggatcgacc ggatcgagca ggaagccgag gcgatgcgcg
     3541 cctatatcca gagcgcctag gccgcggccg gctcgcccct gcgcccgcga atcagcgcca
     3601 cgagctccac caccagcggc cgcgcgaaag ccagcagcag cccgccgtac agcgccgccc
     3661 cggccgccac caggattgcc agccgcggct gcggcggcat cggcggcagc gccccatcga
     3721 gcgccagcac cgccgccgcc atcgccgccg aagcgaggaa gcccggccgc agcgcgcgca
     3781 gcagctcgcc gaggttcagc ccgatggcgg gggcggacag cgccaccgtc gccagcgtca
     3841 gcagcgccat cccccctagc caggcgtaag cgagcccggc cagcccatgg ccgatgccga
     3901 tcaggaacgc ggtcggcagg atcgccgcgc ctacgacgcc ggtcgccagc gcgatgccgg
     3961 gccggccgag cgcgttggtc gccggcgcga acaatatctg cagcgtcagg aacggcatcg
     4021 ccagcgccag cgtcggcacg atcgccacga tccccgccca tttcggcccg aggaaggtca
     4081 gcaccagcgg ctccaccgtc accgccaagc cgaaatagaa aggcagggta accaacatga
     4141 tcagccgcgc cgccttgacg aaggcgccgg ccatcatgtc ccggtccgcc tgcatccgcg
     4201 aataggccgc gaacgcgacc tcgttgagcg gcggcacgaa cttggccgcc aggatctggg
     4261 tcaggaacaa ggcggtggtg tagaggccga gccggtgcgg atcgagcagc cggccggcga
     4321 tgaacacgtc ggactggctc tgcaggaacc agaagaactg gatcgccacc atcgtcccgc
     4381 catagctgaa cagccgcccg gcgccggcga atc
//
LOCUS       DASVRK010000217         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_232394, whole genome shotgun sequence.
ACCESSION   DASVRK010000217 DASVRK010000000
VERSION     DASVRK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232394"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF589_08545"
     CDS             complement(<1..585)
                     /locus_tag="VF589_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX8257671.1"
                     /translation="MAELALVARSDQPQARQEVPPAEAPAGAAEVSAQPPRGRKKRIV
                     GGAGALLLLAGAGLGYQYNAVWAHRETTDNAYVRADITPVAAKVEGYVARLAVGDNQA
                     VEAGDVLMVIEPADYEARVAKAEAELASARANLVTLAAARRSASAQVGAQGGAIGEAS
                     ARVAAARAAAGRAAADEVRFAELAAKGWATRARLD"
     gene            693..941
                     /locus_tag="VF589_08550"
     CDS             693..941
                     /locus_tag="VF589_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257672.1"
                     /translation="MRMRHLEEMAIFARVGDLGSISGAELLRSGDFLHVLPAYKLVDQ
                     PTVYALYAGRTALSPKIGVFLDFLSELAAKVPARPDTS"
     gene            1004..1495
                     /locus_tag="VF589_08555"
     CDS             1004..1495
                     /locus_tag="VF589_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257673.1"
                     /translation="MKHLRSVAGMTLGLCLAACGPGGEAGNAQDSGNAAEAGASAAAE
                     PTVGNDAAAAAGNATAAAAPGLPGCPFRETTNWVGSIEGGKLLVNGSVDLLMGGFQPR
                     LIERPGSAPPVLRLDLALAPGGTTELSNKVRYEKSGSPSYRRGEIYCGGEQVAEFDMI
                     LVG"
     gene            complement(1504..1935)
                     /locus_tag="VF589_08560"
     CDS             complement(1504..1935)
                     /locus_tag="VF589_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEX8257674.1"
                     /translation="MLIGLLALVAAAWFTGAASYINLAEHPARLGLDDAALLRQWQPS
                     YKAALPMQAGLAMLGGALAIWAWARDGDPLWLAGALLLLANWPFTALIIMPTNQRLLA
                     MAPEEAGPGSRAMLLKWGRLHAVRTLLGAAATFLMVGAMLD"
     gene            2082..2732
                     /locus_tag="VF589_08565"
     CDS             2082..2732
                     /locus_tag="VF589_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX8257675.1"
                     /translation="MPRRWNPRPVRRRRAYAGRGWPTAWTAFRSRRVRLSLSEVALTL
                     LLGLALGLAAPYWQARWLPGSDPFAGARPETPNPWAESLRSRAILEPQEGAPAPASAS
                     PSRPATWASDGIVDVIDGDTFDYRGERIRIADIDTPETHPARCAHEAQLGERATQRLA
                     ELLGQGPFLLQAADRDEDRYGRKLRIVTRDGRSLGGVLVAEGLARPWEGRRRSWCG"
     gene            complement(2745..4043)
                     /locus_tag="VF589_08570"
     CDS             complement(2745..4043)
                     /locus_tag="VF589_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8257676.1"
                     /translation="MFTYRFASFAIAFTLATAGAAAPPRESGPIEVIDLPAGRDDGML
                     YIDEETAPVARKADELLDQILSGSGEAGQDLLRPASPLYTALRRGLVRYRQKWGDLPE
                     VEIPAGPTLRVGQEGPRVELLRRRLGLPAGTKFDKALAAAVREYKDAHGLSPDSLVGA
                     ETIASLNLGAAHYERLVAINLERTRSLPVGDRQRYILVDAAAARLWMYENGEVKGTMK
                     VIVGTAASKTPMMAAMIRYADVNPYWNVPPDLTQKRIAPRVLSEGLPYLKGERYEVLS
                     DWTDDAVPVDPAGVDWKAVADGSEVLRVRQLPGVANSMGDIKFMMPNDYGIYLHDTPD
                     KALFEKNDRWLSNGCVRVEDARRLAAWLFGKMPEGQSRDVEERVDLDEPVPVYITYLT
                     AAPADKGGGIAFRADPYERDAPVLARLKASSPIMDTASRD"
BASE COUNT          619 a         1496 c         1464 g          623 t
ORIGIN      
        1 gtcgagccgg gcgcgggtcg cccagccctt cgccgccagc tcggcgaagc gcacttcgtc
       61 ggcggcggca cggcccgcgg ccgcgcgggc ggcggcgacg cgggcgctcg cctcgccgat
      121 cgcgccgccc tgcgcgccca cctgggccga ggccgaacgc cgggcggcgg cgagcgtcac
      181 gaggttggcg cgcgcgctgg cgagctcggc ctcggccttg gcgacgcgcg cttcataatc
      241 ggcgggctcg atcaccatca gcacgtcgcc ggcctcgacc gcctggttgt cgccgacggc
      301 gagcctggcg acatagcctt cgaccttggc cgcaaccggg gtgatgtcgg cccggacata
      361 ggcgttgtcg gtggtctctc gatgcgccca gaccgcatta tattgatagc cgagcccggc
      421 acccgcgagc agcagcagcg cgccggcgcc gccgacgatg cgcttcttgc ggccgcgggg
      481 cggctgcgcg gaaacctcgg cggctcccgc gggcgcctcg gcgggcggga cttcctgccg
      541 cgcctggggc tgatcgctgc gcgccaccaa tgcgagttcg gccatcacac ttcccctgat
      601 tacgtaaacg tcaacccgtt atggcgcgtc ggtcggcctt ccagtagacg gccaatccgg
      661 gatacagcgt tccagctgtg gaacggtggg ggatgcggat gcggcatctg gaggagatgg
      721 cgatcttcgc gcgagtcggc gatctcggct cgatttccgg cgccgagctg ctgcggagcg
      781 gcgacttcct ccacgtcctc ccggcataca aattggtcga ccagccgacg gtctacgccc
      841 tttatgccgg ccgcaccgcc ctctcgccca agatcggcgt gttcctcgat ttcctcagcg
      901 aactcgccgc caaggtgccc gcccgtccgg acacgtcttg atgcaaagcg agcggcaccg
      961 ccgggccggc cgcgggttgt catcgccaac ggaggagaga gggatgaagc atcttcggag
     1021 cgtggccggc atgacgctcg gcctgtgcct ggccgcctgc ggccccggcg gcgaagcagg
     1081 caatgcacag gattcgggca acgcggccga ggcgggcgct tcggcggcag cggagccgac
     1141 ggtggggaac gacgcggccg cagccgccgg caacgccaca gccgcggcgg cgccgggcct
     1201 gccgggctgc ccgttccgcg agaccaccaa ctgggtcggg tcgatcgaag gcggcaagct
     1261 gctggtcaac ggaagcgtcg acctgctgat gggcggcttt cagccgcggc tgatcgagcg
     1321 tccgggctcg gccccgccgg tgctgcggct cgacctcgcc ctcgcgccgg gcggcaccac
     1381 cgagctcagc aacaaggtcc gctacgagaa gagcggatcg ccgagctatc gccgcggcga
     1441 gatctattgc ggcggggagc aggtggcgga gttcgacatg atcctggtcg gctgaggcgc
     1501 cgctcagtcc agcatcgcac cgaccatcag aaaagtcgcg gcagcgccga gcagggttcg
     1561 caccgcgtgc agcctgcccc atttgagcag catcgcccgg ctgccggggc ccgcctcctc
     1621 cggcgccatg gcgagcaggc gttggttggt cggcatgatg ataagcgcgg tgaacggcca
     1681 gttggcgagc agcagcagcg ctccggccag ccagagggga tcgccatccc tcgcccaggc
     1741 ccagatcgcc agcgctccgc ccagcatcgc cagcccggcc tgcatcggca gcgccgcttt
     1801 gtagctcggc tgccactggc ggagcaaagc ggcatcgtcg agaccgagcc gggccggatg
     1861 ctcggccagg ttgatgtaag aagcggcgcc cgtgaaccag gcggccgcca cgagcgccag
     1921 caagccgatc agcatccgcc gtctcccatc cagcccgtgc cactatggcc gaggccggag
     1981 gttccaggca agacggctcc cggcgctcgt tccgctcgcc gtcgatccgt ttcgtagcag
     2041 ccttttacca agccttgaga tacggccgct agcgttggcg ggtgccgaga cgctggaacc
     2101 cccgacctgt ccgccgccgc cgcgcctatg ccggacgcgg ctggccgacg gcgtggaccg
     2161 cctttcgaag ccggcgggtc cggctttcgc tgtccgaggt cgccctcacc ctgctgctcg
     2221 gcctcgcgct cggcttggcg gcgccttatt ggcaagcacg ctggctgccc ggctcggacc
     2281 cgttcgccgg cgcccgcccc gaaacgccca acccctgggc cgaatcgctg cgctcgcgcg
     2341 ccatcctcga gccgcaggag ggagcgccgg cgccggcctc ggcgtccccc agccgccccg
     2401 ccacatgggc ctcggacggg atcgtcgatg tgatcgacgg cgacactttc gactatcgcg
     2461 gcgagcggat ccgcatcgcg gatatcgaca cgcccgaaac ccatccggcg cgctgcgcgc
     2521 atgaagcaca gctcggcgag cgggcgacgc agcggctcgc cgagctgctc gggcagggtc
     2581 cgttcctgct ccaggcggcc gaccgcgacg aggatcgcta cggccgcaag ctcaggatcg
     2641 tcacccgcga cggccggtcg ctcggcgggg tgctggtcgc ggaagggctg gcgcgtccgt
     2701 gggaaggcag gcgccgcagc tggtgcggct gagaacacag tggatcagtc gcgcgaagcg
     2761 gtgtccatga tcgggctcga cgccttcagc cgggccagca ccggcgcgtc gcgctcatag
     2821 ggatcggcgc gaaaggcgat gccgccgccc ttgtccgccg gcgccgcggt caaataggtg
     2881 atgtagaccg gcaccggctc gtcgaggtcg acgcgctcct cgacgtcgcg ggactgcccc
     2941 tccggcatct tgccgaacag ccaagccgcc agccgccgcg catcctcgac cctgacgcag
     3001 ccgttgctga gccagcggtc gttcttctcg aacagcgcct tgtcgggcgt atcgtgaagg
     3061 tagatgccat agtcgttggg catcatgaac ttgatgtcgc ccatcgaatt ggcgacgccg
     3121 ggcagctggc ggacgcgcag cacctcgctg ccgtccgcga ccgctttcca gtcgaccccg
     3181 gccgggtcga ccggcaccgc gtcgtcggtc cagtcggaga gcacttcgta gcgttcgccc
     3241 ttgaggtatg ggaggccctc gctgagcacg cgcggggcaa tccttttctg ggtgaggtcg
     3301 ggcggcacgt tccaataggg attgacgtcg gcatagcgga tcatcgccgc catcatcggc
     3361 gtcttcgacg ccgcggtgcc gacgatcacc ttcatcgtgc ctttgacctc gccattctcg
     3421 tacatccaca gccgcgccgc ggcggcgtcg accagaatgt aacgctggcg gtcgccgacg
     3481 ggcagcgagc gcgtgcgctc gagattgatc gccaccagcc gctcatagtg cgcggcgccg
     3541 aggttgagcg aggcgatggt ttccgcgccc accaggctgt cgggcgagag cccgtgcgcg
     3601 tccttatatt cgcgcaccgc cgccgccagc gccttgtcga acttggtccc cgcgggaagg
     3661 ccgagccggc gccggagcag ctcgacccgc ggaccctcct ggcccacgcg cagcgtcggt
     3721 ccggccggaa tctcgacctc aggcaggtcg ccccatttct ggcggtagcg gacgaggccg
     3781 cggcgcaagg cggtgtaaag cgggctcgcc gggcgcagca gatcctggcc ggcctcgccc
     3841 gagcctgaca agatctggtc gagcaattcg tcggccttgc gcgcgaccgg agcggtctcc
     3901 tcgtcgatat agagcatgcc gtcgtcgcgg ccggcgggaa ggtcgatcac ctcgatcggc
     3961 ccgctttcgc gcggcggcgc ggccgcgccg gccgtggcaa gcgtgaacgc gatcgcgaac
     4021 gaagcaaatc ggtacgtaaa cactgacccc aatccccctt cgcgaacgcc tatgcgcccc
     4081 cgatacttaa cgcgctccga ataagcggca gggcggggcc cgggccaagc gctattccgc
     4141 agggcgcccc tgacgcgcgg cggggcgagt ctgttcgatc aaaggtggcg gcgaaggtgg
     4201 tg
//
LOCUS       DASVRK010000218         9881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_480725, whole genome shotgun sequence.
ACCESSION   DASVRK010000218 DASVRK010000000
VERSION     DASVRK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480725"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..721)
                     /locus_tag="VF589_08575"
     CDS             complement(86..721)
                     /locus_tag="VF589_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257677.1"
                     /translation="MTPAARVQAAIELLDEIIAAAGGGGAAADTIVSAYFRTRRYAGS
                     KDRRAVRELVYRTIRRSGEIPASGRAALLGLTEEEPALRDLFDGSAHGPAPPLPDEPV
                     AAARAVPAWIEPRFDPLIEAGEFHPLLERAPLDLRANRLRGTIEDALAAFPDASPTPL
                     SPLGLRLPEGTQVEPSPAWRSGLVEVQDEGSQLLALATARRKSRRSGRGPG"
     gene            complement(731..2188)
                     /gene="guaB"
                     /locus_tag="VF589_08580"
     CDS             complement(731..2188)
                     /gene="guaB"
                     /locus_tag="VF589_08580"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX8257678.1"
                     /translation="MDISLGLTFDDVLLRPAESNVLPSQADTRTRVTKEIALSIPILS
                     SAMDTVTEADMAIVMAQLGGIGVLHRNLSVEGQAAAVRAVKRYESGMVVNPITMTAEQ
                     TLADALELMKRHKISGIPVTDAGGRLVGILTNRDVRFAENPKQPVAELMTRDNLVTVG
                     PGVTQEEARRQLHQRRIEKLLVVDEQQRCIGLITVKDIEKAVNYPSATKDATGRLRVA
                     AATTVGDKGFERSEALIDAECDLIVIDTAHGHNRDVGVAVERIKKLSNSVQVVAGNVA
                     TAEATKALIGAGADAVKVGIGPGSICTTRIVAGVGVPQLTAILEAAGEAARSGTPVIA
                     DGGVRTSGDVAKALAAGASTVMIGSLLAGTEEAPGEIFLYQGRSYKSYRGMGSVGAMA
                     RGSADRYFQQDIKDQLKLVPEGIEGQVPYKGHASDVIHQLVGGVKAAMGYTGSATIED
                     LQRRAQFVRITNAGLRESHVHDVSITREAPNYPTR"
     gene            complement(2266..3075)
                     /locus_tag="VF589_08585"
     CDS             complement(2266..3075)
                     /locus_tag="VF589_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX8257679.1"
                     /translation="MDTARLRLCRSLLFLPASNPRAIDKARTLEADMIVLDLEDAVKP
                     EEKATARTAAETAVGEGFGDRLAAIRMNTVGSEYYGEDVVAIRSSSAEFVILPKAESR
                     EQVHDTRRLVERPVLAMVETPAGVLAARAIAAEAAALVAGTNDLAAELGIPVSADRRG
                     LTLGLQTIVLAARAAGIPAFDGVYNGLDDDEGLARQCEEGRAFGFDGKSLIHPSQIAT
                     ANRLFGPSEAELDAAARLVAAATGGAERHEGRMIEAMHVAQAEALLAKGRR"
     gene            3151..4134
                     /locus_tag="VF589_08590"
     CDS             3151..4134
                     /locus_tag="VF589_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8257680.1"
                     /translation="MEVTAMFFVLVLTIIYLMASIKIVRQGYQYTIEYFGRFTTVARP
                     GFNFYPAFFYRVGRKVNMMEQVLDIPGQEIITKDNAMISTDGVVFFQVLDAAKAAYEV
                     SDLYNAILNLVTTNLRTVMGSMDLDETLSKRDEINARLLAVIDDATTPWGVKITRIEV
                     KDIRPPTDIVNSMARQMKAEREKRASILEAEGMRASEILRAEGQKQSQILQAEGRREA
                     AFREAEARERAAEAEAKATQMVSDAIAGGNTQAINYFVAQKYIEAVARFATSPNAKTI
                     LFPVEATQLIGTLGGIGELAREALGERAAPPPAPAPAPAPRRAGPFEEVKR"
     gene            4131..4601
                     /locus_tag="VF589_08595"
     CDS             4131..4601
                     /locus_tag="VF589_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX8257681.1"
                     /translation="MNLDGIEPHWLWLIAAALLGIAELLVPGVFLVWLAAAAAVTGFA
                     ALLLGIPPAFQFALFALLAIAAVYFGRRWYAANPVESSDPLLNDRAARLVGQTVVVVS
                     AFEDGRGRVKVGDSVWAAMGPDAEVGARVRVTGAQGTCLRVEKVEPAGGTLPAR"
     gene            complement(4800..5033)
                     /locus_tag="VF589_08600"
                     /pseudo
     CDS             complement(4800..5033)
                     /locus_tag="VF589_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
     gene            complement(5146..5775)
                     /locus_tag="VF589_08605"
     CDS             complement(5146..5775)
                     /locus_tag="VF589_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257682.1"
                     /translation="MIQPLMPSKFRRLALAPSAALCGLTMAVPSPIAAAGAAESDRDS
                     YVLLAPGSRSSTMSGSIEDMNRARALRTGQEGLLYVRQGGVAIRDSATLRQAQAIFEP
                     QQALGARQGELGSRQAALGRRQASLGKEQARLGLQQASASPGRGDELGRRQDALGRQQ
                     DELGEQQDVLGRQQDALGREQDRLGRIADAKFRALVADATRRGVAQRVD"
     gene            complement(5978..6118)
                     /locus_tag="VF589_08610"
     CDS             complement(5978..6118)
                     /locus_tag="VF589_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX8257683.1"
                     /translation="MDPIAKRTAIAAAIGAAIAIPVPFVGPLFGAAVGAGIGYFSAKR
                     RG"
     gene            complement(6208..6657)
                     /locus_tag="VF589_08615"
     CDS             complement(6208..6657)
                     /locus_tag="VF589_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257684.1"
                     /translation="MTEEHEPRLFADDAAVRRIGEGLIACSLTRPDWTHEAHLAACLY
                     ILTERPDIAPERDLPGLIRRFNESVGGVNSDTEGYHETITQLYIKGVRAFLARTDATL
                     PLATKVNLLLRAEEGRRDWPLRFYSAERLFSKEARLGWVEPDLNALA"
     gene            complement(6743..8374)
                     /locus_tag="VF589_08620"
     CDS             complement(6743..8374)
                     /locus_tag="VF589_08620"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX8257685.1"
                     /translation="MATRVPAHGLAAQGIETSAQLHWNLGTAALVEQAVQRGEGVLAK
                     DGPLVVETGKHTGRSAKDKFTVRDAETESSLWWDNNKAMSPDHFAALKADFLAELARK
                     ETLFVADLHGGSQPEHRVNVRVINELAWHNLFIRTLLVRPEAAELDGFAPDFTIIDLP
                     SFVADPARHGCRSETVIAVNLSEKLILIGGTKYAGEMKKSVFGVLNYLLPPKGVMPMH
                     CSANIGPAGDTAVFFGLSGTGKTTLSADPARTLIGDDEHGWSDTAVFNFEGGCYAKMI
                     RLSAEAEPEIYATTKRFGTVLENVVIDPATRELDLDDPSLAENSRGAYPIDFIPNCSD
                     RNMGPVPRNIVMLTADAFGVLPPIARLTPDQAMYHFLSGYTAKVAGTEIGVTEPEATF
                     STCFGAPFMPRHPSVYGNLLKERIARGHVSCWLVNTGWTGGKYGTGKRMPIKATRALL
                     NAALDGSLERAEFRIDENFGFEVPVSVPGVHAAILEPRETWADKAEYDATAAKLVKLF
                     IDNFAKFEPHVDAGVRDAAPRAQAQAQQEPTATAA"
     gene            8541..9248
                     /locus_tag="VF589_08625"
     CDS             8541..9248
                     /locus_tag="VF589_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8257686.1"
                     /translation="MSVTIALVDDDRNILTSVSIGLQAEGFITRVYTDGETALKALLD
                     NPPDLAVLDVKMPRMDGMELLRRLREKSALPVIFLTSKDDELDEALGLAMGADDYIAK
                     PFSQRLLIARIRAILRRTEMRATPVEEQPAEEPPQIVRGRLAMDPARHRVTWDGKDVT
                     LTVTEFMILEALAQRPGVVKSRNQLMDVAYQDDIYVDDRTIDSQIKRLRRKFRAVDGE
                     FGAIETLYGVGYRFAEE"
     gene            9235..>9881
                     /locus_tag="VF589_08630"
     CDS             9235..>9881
                     /locus_tag="VF589_08630"
                     /inference="COORDINATES: protein motif:HMM:NF025130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor N-terminal transmembrane
                     domain-containing protein"
                     /protein_id="HEX8257687.1"
                     /translation="MPRNEERALTLRWTGRLSLTKRILAVNIFALAILAGSLFYLDSF
                     RGRLTAARLAQARSEAVLIADVLAVAAPGTRRALLRELGAESGTRLRVYGRDGAKRMD
                     SWAGARPTYELRDPARESWDKDVARLMDNGFDAVVGARQPPPLVTPGVDRLAAWPEAA
                     AALATGAPATALRRAPEGTAYVSAAAGTEIEGGAALLLTVNARDIRRVVRSERMTL"
BASE COUNT         1482 a         3280 c         3508 g         1611 t
ORIGIN      
        1 gaagcgttcg ggcgccgcca ttcatctttg gtatcggatt gcgagcatat cctgtgggga
       61 aggccgtcgg gtccgggccg tctgctcagc ccggcccacg accggaccga cggctttttc
      121 gtcgcgcggt ggcgagcgcc agcaactggc tgccctcgtc ctggacttcg acgagcccgg
      181 accgccaggc ggggctcggc tcgacctgcg tcccctccgg caggcgaagg ccgagcggcg
      241 acagcggcgt cgggcttgcg tcggggaagg ccgccagcgc gtcctcgatc gtgccgcgaa
      301 ggcggttggc gcgaaggtcg agcggtgcgc gctcgagaag ggggtggaac tcgcccgcct
      361 cgatcagcgg gtcgaaacgg ggttcgatcc aggcgggcac ggcgcgggcg gcagcgacgg
      421 gctcgtccgg tagcggcgga gcgggcccat gggccgagcc atcgaacagg tcgcgaagcg
      481 ccggctcctc ctcagtgagg ccgagcaggg cggcgcggcc cgaggcgggg atctcgccgg
      541 agcggcggat tgtgcgatag accaattcgc gcaccgcgcg acggtccttg gagccggcat
      601 agcggcgggt ccggaaatag gccgagacga tggtgtcggc ggcggctccg cccccaccgg
      661 ccgcggcgat gatctcgtcg agcagctcga tcgcggcctg cacccgcgcg gcgggggtca
      721 cggcgatatg ctagcgggtg gggtaattgg gcgcctcgcg ggtgatcgag acgtcgtgga
      781 catggctttc gcgcaggccc gcattggtga tgcggacgaa ctgggcgcgg cgctggaggt
      841 cctcgatggt ggcggagccg gtatagccca tcgcggcttt gacgcccccg accagctggt
      901 ggatgacgtc gctggcatgg cccttatagg ggacctgacc ctcgatcccc tccggcacca
      961 gcttcagctg gtccttgatg tcctgctgga aatagcggtc ggccgacccg cgcgccatcg
     1021 cgcccaccga gcccatgccg cgatagctct tgtaggagcg gccctggtaa aggaagattt
     1081 cgcccggcgc ctcctcggtg ccggcgagca gcgatccgat catgacggtc gaggcgccgg
     1141 ccgccagtgc cttggcgacg tcgccgctgg tccggacgcc gccgtcggcg atgacggggg
     1201 ttccgctccg cgccgcctcg ccggccgcct cgagaatggc ggtgagctgc ggcacgccga
     1261 cgccggcgac gatgcgggtg gtgcaaatcg agccgggacc gatgccgacc ttgaccgcgt
     1321 ccgccccggc tccgatcagc gccttggtcg cctcggccgt ggcgacgttg ccggcgacga
     1381 cctgaaccga gttggagagc ttcttgatcc gctcgaccgc gacgccgacg tcgcgattgt
     1441 ggccgtgggc ggtgtcgatg acgatgaggt cgcattcggc gtcgatcagc gcttcggagc
     1501 gctcgaaccc cttgtcgccg accgtggttg cggcggcgac gcgcaggcgt ccggtcgcgt
     1561 ccttggtcgc ggagggataa ttgacggctt tctcgatgtc cttgacagtg atgaggccga
     1621 tgcagcgctg ctgctcgtcc accaccagca atttctcgat ccggcgctgg tgaagctgcc
     1681 gccgcgcctc ctcctgggtg acgccgggac cgacggtgac gagattgtcg cgagtcataa
     1741 gctccgcgac cggctgcttg gggttttcgg cgaagcggac gtcgcgattg gtgaggatgc
     1801 cgaccagcct gccgccggcg tcggtgacgg ggatgcccga aatcttgtgg cgcttcatca
     1861 gctcgagcgc gtcggcgagg gtctgctcgg cggtcatcgt gatcgggttc accaccatgc
     1921 cgctctcgta gcgcttcacc gcccgtaccg ccgccgcctg cccttcgacc gagaggttgc
     1981 ggtgaagtac gccgatgccg ccgagttgcg ccattacgat cgccatgtcg gcttcggtga
     2041 cggtgtccat ggccgacgac aggatcggga tgctgagcgc gatctccttg gtcacccggg
     2101 ttcgggtgtc cgcctggctc ggcagcacgt tcgattcggc gggccggagc agcacgtcgt
     2161 cgaaggtgag cccgaggctg atgtccatgg atgcgtctcg cgtgtcgggt ttcggtgccg
     2221 ctacataggg atggaatgtg acaagcgcca gcggctggcc ggccgctatc gccgtccctt
     2281 ggcgagcaag gcttcggcct gggcgacgtg catcgcctcg atcatgcggc cctcgtgacg
     2341 ctcggcgccg ccggtggcgg cggcgaccag ccgggcggcg gcgtcgagct cggcctcgct
     2401 cggcccgaac aggcggttag cggtcgcgat ttggctcggg tggatgagcg acttgccgtc
     2461 gaagccgaac gccctcccct cctcgcactg gcgggccagc ccctcatcgt cgtcgagccc
     2521 gttatagacg ccatcaaagg cggggatgcc ggcggcgcgc gcggcgagga cgatggtctg
     2581 gagccccaaa gtcagcccgc gccggtcggc cgagaccggg atgccgagct cggcggcaag
     2641 gtcgttggtg ccggcgacca aggcggccgc ttcggcggcg atcgcccggg cggcgagcac
     2701 gccggccggg gtttcgacca tcgccagcac cggtcgctcg accagccggc gcgtatcgtg
     2761 gacctgctcg cggctctcgg ccttgggcag gatcacgaac tcggccgagc ttgagcggat
     2821 cgcgaccacg tcctccccgt aatattcgct gccgaccgtg ttcatccgga tcgcagccag
     2881 acggtcgccg aacccctcgc ccaccgccgt ctccgcggct gtgcgggcgg tcgccttctc
     2941 ctccggcttc acagcatctt cgaggtcgag cacgatcatg tccgcctcca gcgtccgcgc
     3001 cttgtcgatc gcgcgcgggt tggaggcggg caggaacagg agggagcgac aaaggcgcag
     3061 gcgggcggtg tccatcgcct ccgattctgc ttggaagagg ggccgcttgt ctacatgata
     3121 tcgtcggcaa gcgttcggga ggctaaggcg atggaagtga cggcaatgtt cttcgtcctg
     3181 gtgctgacca tcatctacct gatggccagc atcaagatcg tccgtcaggg ctatcaatat
     3241 acgatagaat atttcggccg cttcaccacg gtcgcgcgcc cgggcttcaa tttctacccg
     3301 gccttcttct accgcgtcgg ccgcaaggtg aacatgatgg agcaggtgct cgacataccg
     3361 ggccaggaga tcatcaccaa agacaatgcg atgatctcga ccgacggcgt ggtgttcttc
     3421 caggtgctgg acgcggccaa ggcggcctac gaagtgtccg acctctacaa cgccatcctc
     3481 aatctggtca ccaccaacct caggaccgtg atgggctcga tggacctcga cgagaccctg
     3541 tcgaagcgcg acgagatcaa cgccagactg ctggcggtga tcgacgacgc gaccacgcct
     3601 tggggcgtca agatcacccg cattgaggtc aaggacatcc ggccgccgac cgacatcgtc
     3661 aattcgatgg cgcgccagat gaaggccgag cgcgagaagc gcgcctcgat cctagaggcc
     3721 gaaggcatgc gcgcctcgga gatcctccgt gccgaaggcc agaagcagtc gcagatcctg
     3781 caggccgagg gccgccgcga ggccgcgttc cgcgaagccg aggcgcgcga gcgggcggcc
     3841 gaagccgaag ccaaggcgac ccagatggtc tccgacgcca tcgccggcgg caacacccag
     3901 gcgatcaact atttcgtcgc ccagaaatat atcgaggcgg tggcgcgctt cgccacctcg
     3961 cccaacgcca agacgatcct gttcccggtc gaggccacgc aattgatcgg cacgttgggc
     4021 gggatcggcg aactcgcccg cgaagcgctc ggcgagcgcg ccgcgccgcc gccggcaccg
     4081 gcccccgccc cggcgccgcg tcgcgccggc ccgtttgaag aggtcaagag atgaacctcg
     4141 acgggatcga gccgcactgg ctgtggctga tcgccgcggc cctgctcggc atcgccgaat
     4201 tgctggtgcc gggcgtgttc ctggtctggc tggccgccgc cgccgcggtg accggcttcg
     4261 cggccttgct gctcggcatc ccgcccgctt tccagttcgc cttgttcgcg ttgctcgcca
     4321 tcgccgccgt ctatttcggc cgccgctggt atgccgccaa cccggtcgag agcagcgatc
     4381 cgcttctcaa cgaccgcgcc gcccgcctgg tcggccagac ggtggtggtg gtgagcgcgt
     4441 tcgaggacgg ccgcggccgc gtcaaggtcg gcgacagcgt gtgggcggcg atgggtcccg
     4501 acgccgaagt aggcgcacgg gtccgggtga cgggggcgca ggggacctgc ctccgggtcg
     4561 agaaggtcga gccggcgggc gggacgctgc cggcccggta gagccgctta gtaagcggga
     4621 ggctttcaca gggctgggcc ccagccttcg ccggggcaca agtggcctgc agatcagtcg
     4681 tcgcggcccc agcgctgcat cagggcggcg ccggcggcgg cggcgacgcc gaccgcgacc
     4741 gcaccgaggc cgatcgtggt ccagttgaac aagcggcttt cgccgaggaa ctggccgcct
     4801 ttctcctcct cgagcagcac cgccgcgtcg ccattggcgg acagccgcac cttgttgcct
     4861 tcgatgccgg cgatcagtcc gccagagata taatggtggt ggctctggtg cgagccgctg
     4921 tcggccttgg tcatcttgat gcggtggccc tcgaccttgt ccacggtgcc gatatggacg
     4981 ccgtcggccc cgataatttc catatgctcg cgaacctggc tgatgtcggc catcaatcgt
     5041 ctcctgctgg cggtggtttc gaagccataa cgataactgg cggcgatgga tgcgccgctc
     5101 gggatggccg gtcaagtccg gcgtgacgag tttgaagaga ccgcttcagt cgacgcgctg
     5161 ggccacgccg cgccgggtgg cgtcggcgac cagggcgcgg aacttggcgt cggcgatgcg
     5221 gcccaggcgg tcctgctcgc ggccgagcgc gtcctgctgg cggccgagca cgtcctgctg
     5281 ctcgccgagc tcgtcctgct gccgtccgag cgcgtcctgc cggcggccga gctcgtcccc
     5341 gcgccccggc gacgcgctcg cttgctgaag gccgagccgg gcctgctcct tgccgaggct
     5401 cgcctggcgc cggccgagcg ccgcctggcg cgaacccagt tcgccctgtc gcgcgccgag
     5461 cgcctgctgc ggctcgaaaa tggcttgggc ctggcgcaac gtcgcggagt cgcggatggc
     5521 gacgccgccc tggcgcacgt agagcagccc ctcctggccc gtccgcagcg cacgggcgcg
     5581 gttcatatcc tcgatcgatc cgctcatcgt gctgctccgg ctccccggag caaggaggac
     5641 ataggagtcg cggtcggact cggccgcacc cgcggccgcg attggcgaag ggacggccat
     5701 ggtcaggccg caaagcgcgg ccgacggggc caaagcgagg cggcggaact tgctgggcat
     5761 gagcggctgg atcacatgtc gtcctttcga ttgcggcgct ggccgatacg atcggcgacg
     5821 ctgcaacttt cgggccagcc cgcccctatg gcgccgcgca cggcaagagg gacctcgctg
     5881 ttcgctggcg gacacttcag ggttcggatt cgaacaactc gcggcccgtt tcgtgacgcc
     5941 gccttgatcc ggaccgcgtc cgcaggcgct gtccgcctca gcctcgccgc ttggccgaga
     6001 aatagccgat gccggccccg accgccgctc cgaacagcgg cccgacgaac ggcaccggaa
     6061 tggcgatcgc cgcgccgatg gccgccgcaa tcgccgtccg cttcgcaatg ggatccatgg
     6121 cctttacctc ctgaagctgt attataggta taatacagct tctcgtctag ccctggctta
     6181 tctactcccg gccgtgctcg gcaccaatca agccaaagca ttcagatcgg gctccaccca
     6241 cccaagccgc gcctccttcg agaacagacg ctcggcgctg tagaagcgga gcggccaatc
     6301 ccgcctgccc tcctccgccc gcagcaaaag attgaccttc gtggcgagcg gcagcgtggc
     6361 atcggtgcgg gcgaggaagg cgcggacgcc cttgatgtag agctgggtga tcgtctcgtg
     6421 atagccttcg gtgtcgctgt tgacgccgcc gacgctctcg ttgaagcggc ggatgaggcc
     6481 ggggaggtcg cgctcggggg cgatgtcggg gcgctccgtc aggatataga ggcaggcggc
     6541 gagatgcgcc tcgtgggtcc agtctgggcg ggtcaagctg caggcgatca gcccctcccc
     6601 gatccgcctt accgccgcgt cgtcggcgaa caggcgcggt tcgtgttcct cggtcatgtg
     6661 ttcctccgtt gaaggcatag aaaaagggcg cgccggcctg tcgccgacgc gccctttcgg
     6721 gtcctcttga gtggctaaag cgtcaggcgg cggtcgcggt gggctcctgt tgggcttgcg
     6781 cctgcgcgcg cggcgcggcg tcgcggacgc cggcgtcgac atggggctcg aacttggcga
     6841 aattgtcgat gaacagcttg accagcttgg ccgcggtggc gtcgtactcg gccttgtcgg
     6901 cccaggtctc gcgcggctcg aggatggcgg catggacgcc ggggacgctc accggcactt
     6961 cgaagccgaa attctcgtcg atgcggaatt cggcgcgctc gaggctgccg tcgagggcgg
     7021 cgttgagaag cgcgcgggtg gccttgatcg gcatgcgctt gccggtgccg tatttgccgc
     7081 cggtccagcc agtattgacc agccagcagg agacgtggcc gcgggcgatc cgctccttca
     7141 acagattgcc gtagacgctc gggtggcgcg gcatgaaggg cgcgccgaag caggtcgaga
     7201 aggtcgcctc gggctcggtg acgccgatct cggtgccggc gaccttggcg gtgtagccgg
     7261 agaggaaatg atacatggcc tggtcgggcg tgagccgcgc gatcggcggc agcacgccga
     7321 acgcgtcggc ggtgagcatg acgatgttgc gcggcaccgg ccccatgttg cgatcggagc
     7381 aattggggat gaaatcgatc ggataggcgc cgcggctatt ctcggcgagg ctcggatcgt
     7441 cgaggtcgag ctcgcgcgtc gccgggtcga tgacgacatt ttccagcacg gtgccgaagc
     7501 gcttggtggt ggcgtagatt tccggctcgg cctcggccga caggcggatc atcttggcgt
     7561 agcagccgcc ctcgaagttg aacacggcgg tgtcggacca gccatgctcg tcgtcgccga
     7621 tcagggtgcg ggcgggatcg gccgagagcg tggtcttgcc ggtgcccgaa aggccgaaga
     7681 agacggcggt gtcgccggcg gggccgatat tggccgagca gtgcatcggc atcacgccct
     7741 tgggcggcag caggtagttg aggactccga acaccgactt cttcatctcg ccggcatatt
     7801 tggtgccgcc gatcaggatc agcttctcgc tcagattgac ggcgatcacg gtctcggagc
     7861 ggcagccgtg gcgggcggga tcggcgacga agctcggcag gtcgatgatc gtgaaatcgg
     7921 gcgcgaagcc gtcgagctcg gcggcctcgg ggcgcaccag caaggtgcgg atgaagaggt
     7981 tgtgccaggc gagctcattg atgacgcgca cgttgacgcg gtgctcgggc tgcgagccgc
     8041 catgaaggtc ggcgacgaac aaggtctcct tgcgcgccag ctcggcgagg aagtcggcct
     8101 tgagggcagc gaaatggtcg gggctcatcg ccttgttatt gtcccaccag aggctgctct
     8161 cggtctcggc gtcgcggacc gtgaacttgt ccttggcgga gcggccggta tgcttgccgg
     8221 tctcgaccac cagcggtccg tccttggcaa gcacgccctc gccgcgctgc accgcctgct
     8281 cgacgagggc ggcggtcccc aaattccagt ggagctgagc ggacgtttcg atgccctggg
     8341 cggcaagtcc atgggcgggc acgcgagtgg ccaagtcgat ctcctgtttc ctgcacgact
     8401 gaaggcggca cgaaacgcgc cgcaacgaag ttgccgcccg catatccaac gagatgcgcc
     8461 ccgtcaaacc gaggggaccc gcggttgcgg ctccgtcccg ctttggataa agccgggcgg
     8521 atgcgaccag gggcgttcga atgtcggtaa cgatcgcgct ggtcgacgat gaccgcaaca
     8581 tcctcacctc ggtgtcgatc ggactccagg ccgagggttt catcacccgg gtctatacgg
     8641 acggggagac ggcgctgaag gcgctgctcg acaacccgcc cgacctcgcc gtcctcgacg
     8701 tcaagatgcc gcggatggac ggcatggagc tgctccggcg gctgcgtgag aagagcgccc
     8761 tccccgtcat cttccttacg tccaaggacg acgagctcga cgaggcgctc ggcctcgcca
     8821 tgggcgccga cgattatatc gccaagcctt tctcgcagcg cctgctcatc gcccgcatcc
     8881 gcgcaatcct gaggcggacc gagatgcgcg ccacgccggt cgaggagcag ccggccgagg
     8941 agccgccgca gatcgtgcgc gggcggctcg ccatggaccc cgcccgccac cgcgtcacct
     9001 gggacggaaa ggacgtgacg ctgaccgtga ccgagttcat gatcctggag gcgctcgcgc
     9061 agcggccggg ggtggtgaaa tcgcgcaacc agctgatgga cgtggcctac caggatgaca
     9121 tctatgtcga cgaccgcacc atcgacagcc aaatcaagcg cctgcgccgc aagttccgcg
     9181 cggtcgacgg cgagttcggc gcgatcgaga cgctctacgg cgtcggatac cgctttgccg
     9241 aggaatgagg agcgggcgct gacgctgcgc tggaccgggc ggctgtcgct caccaagcgg
     9301 atcctcgccg tcaacatctt cgcgctggcg atcctggccg gcagcctgtt ctatctcgac
     9361 agcttccgcg gccggctcac cgccgcgcgg cttgcccagg cgcgcagcga agcggtgctg
     9421 atcgccgacg tgcttgcggt ggcggcgccg gggacgcggc gcgccttgct gcgcgagctg
     9481 ggcgccgaat cgggcacccg gctgcgggtc tacggccggg acggcgccaa gcggatggac
     9541 agctgggccg gcgcgcgccc gacctacgag ctgcgcgacc cggcgcgcga gtcctgggac
     9601 aaggatgtgg cgcggctgat ggacaacggc ttcgacgccg tggtcggagc gcggcagccg
     9661 ccgccgctgg tcacgccggg ggtcgaccgg ctggcggcct ggcccgaagc ggccgcggcg
     9721 ctggcgaccg gcgctccggc caccgccctc cgccgcgcgc ccgaaggcac cgcctatgtc
     9781 tcggccgcgg cggggacgga gatcgagggc ggggcggcgc tgctgctcac cgtcaatgcc
     9841 cgcgacatcc gccgggtcgt gcgcagcgag cgcatgaccc t
//
LOCUS       DASVRK010000219         6940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_418263, whole genome shotgun sequence.
ACCESSION   DASVRK010000219 DASVRK010000000
VERSION     DASVRK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418263"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VF589_08635"
     CDS             <1..489
                     /locus_tag="VF589_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8257688.1"
                     /translation="IRIENVYIPAGGDIPDRNENPKFGQKLDFIERMTRWSEDCRCPT
                     LLVGDFNVAPLECDVWSHKQLLNVVSHTPVEVEALTRLQQSGEWVDLGRRFFPAPGRL
                     HTWWSYRSPDWTRNDRGRRLDHMWATPDVAALAVGHKVHEPCRNWTRPSDHVPLVTEF
                     AV"
     gene            486..1535
                     /gene="ribA"
                     /locus_tag="VF589_08640"
     CDS             486..1535
                     /gene="ribA"
                     /locus_tag="VF589_08640"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX8257689.1"
                     /translation="MSGGRDLARAIDALRRGWPVVIDGSGFLAVETADDAGLAAFDAG
                     GPADLLISGNRAVTLKLANQRDAVPTGPVRIVRSPWLDLAEATAVADPALDLKTPLKG
                     PFRTIPLGCDKAALAAITLTRRAGLLPALFIDGDAGADAISIDAAAVAAFDKSPSLAI
                     ASRAKLPTRAAEQAEIVAFRSEEDAAEHVALLIGQPNGQPPLVRLHSECLTGDVLGSL
                     KCDCGPQLDAAIAEIRRAGWGILLYLRQEGRGIGLVNKLRAYALQDQGFDTVDANHRL
                     GFGDDERDYGVAARMLAKLSQTEVALLTNNPRKAAGLRAEGIDVVRQVPLKVGANAFN
                     RDYLDTKRARSGHQL"
     gene            complement(1546..2217)
                     /locus_tag="VF589_08645"
     CDS             complement(1546..2217)
                     /locus_tag="VF589_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX8257690.1"
                     /translation="MTFGAIIFDFDGVIVDSEVVANAALAELLTEAGHPVTADHALAY
                     YSGLRWSDCHRRIEEESGLSFDPDQLGDMLDQAIAARVADILAIEGIQKFLAAQAHRS
                     LAIASSSEKDWLDASLARLGLGHYFEERVYSAAGLARGKPHPDIYLGVAERLGIAPPR
                     CLVIEDHPVGVAAGAAAGMTVIALLAGGHIGPGHGDRARAAGAHHVARDYAEVAEILA
                     RLERE"
     gene            complement(2219..2887)
                     /locus_tag="VF589_08650"
     CDS             complement(2219..2887)
                     /locus_tag="VF589_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX8257691.1"
                     /translation="MSEASAAGRLGEVRAAIARSAALAGRSQGEVALIAVSKTHPPEA
                     IVPLLEAGHRIFGENRVQEAQAKWPELRERYEGVELHLIGRLQSNKADEVAELFDAIH
                     SLDRPSLIPALAAAMAKAGRRPDCFIQVNIGDEAQKGGCAVAELPALLDAAREAGLPV
                     RGLMCIPPAELEPAPYFALLAKLARRHALPGLSMGMSADFETAVTIGATHVRVGTALF
                     GERG"
     gene            2910..3461
                     /locus_tag="VF589_08655"
     CDS             2910..3461
                     /locus_tag="VF589_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX8257692.1"
                     /translation="MHRRQPLPRLWLMTDERQGDALCSALERLPRGSGVVFRHYGLPT
                     PERRRLFGQVRAVARRRRLVLLLAGTPRLAAAWGADGSHGRGSHAPLRGQVRTAPVHD
                     VRDMIAAARAGADLLFVSPAFGTRSHPGAAPLGRVRFGLLARQARGPVVALGGMTPSR
                     FRGLAAFRIYGWAAIDAWSAGPA"
     gene            complement(3458..4159)
                     /locus_tag="VF589_08660"
     CDS             complement(3458..4159)
                     /locus_tag="VF589_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257693.1"
                     /translation="MGGASLVALSLLLAPAFAASNQPQKAKKARSAPSFGSSGFTPAV
                     ADPRLAAEFARRGLQANSFRFTPSSASPDKSKAVRVAVRARATTAAEAVRSAAATAET
                     SASPVTAITPTAYNLGVAVGWKQFALSGDVAKVQGGPIPGGREAAEVGVSYLGKKFTG
                     RVAVGAERTDGNQLRIMPQESSYSLDVGGSYSIARNVDVTGGVRYKIQRERLEPLADE
                     RRDSQAVYVGTAFRF"
     gene            complement(4345..6435)
                     /locus_tag="VF589_08665"
     CDS             complement(4345..6435)
                     /locus_tag="VF589_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8257694.1"
                     /translation="MTNILNGPRLAALVAVTLGAPAGTAAAQAQVPAEAAPALPAAAE
                     GARSYAPADFARFSPKTALDMLRQVPGFTIQEGDDERRGLGQAATNVLINGSRFSGKS
                     NDVVTEVGRIAAANVVRIDIVDGATLDVPGLSGQVANIVTAASGVSGSFAWRPQYRPK
                     FDKLRLTNGEASVSGAALGFDYTLSMANESYIGAEEGPELVFTPDGTVTDRRAETLTY
                     EGEVPRLGASLRREAGGTLLNLNAAVALDYQDLREVSLRSFPGLVDRDRRLEERRRKW
                     EYELGGDYEFGLGGGRLKLIGLRRFEHTPFRQRLTIDFADERASAGDLFTQTADEAET
                     IARAEYRWQAGAADWQVSAEGALNILDIANSLARLDAAGQWVPVPLPGSAARVDEKRG
                     EVMLSYGRPLSPVLSLQASLGGEYSQLSQSGENGLARTFLRPKGLISVAWKASPRLDV
                     SAKLERVVGQLNFFDFVASSNVSAGTVNAGNPNLVPPQSWDAEVEATRNLGRWGTATA
                     RVYGRLISDIVDVIPVGDDGQSPGNLDSATLYGVQMTGTFNFDPLGWRGAKLDLDLQF
                     QRSSLEDPVTGRNRGINDTMTRRIVANLRHDVPGTPWAWGAGYDEYRQSAGYRLDQRL
                     QFLNAPGILSAFIEHKDVLGLTVRGTVENLIGQNVQLTRDFHDGRRTGPILFTEDRNR
                     DIGPILTLAISGKI"
     gene            6603..6818
                     /locus_tag="VF589_08670"
     CDS             6603..6818
                     /locus_tag="VF589_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257695.1"
                     /translation="MSKNNHNHNAMQFTQNQNNEMSDDPGDGSRTSFTLGKDGTSPVE
                     KKPPNDAQDRSTIEAFGEEGAGIAAKE"
     gene            6824..>6940
                     /locus_tag="VF589_08675"
     CDS             6824..>6940
                     /locus_tag="VF589_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8257696.1"
                     /translation="MSDTETLEMIHEDQLPGHESQLEPKPDWEPRYPGSGRLV"
BASE COUNT         1039 a         2373 c         2474 g         1054 t
ORIGIN      
        1 ataaggatcg agaacgtcta catccccgcc ggcggcgaca ttccggaccg caacgaaaat
       61 cccaagttcg gccagaagct cgacttcatc gagcggatga cgcgctggtc ggaggattgc
      121 cgctgcccga ccctgctcgt cggcgacttc aacgtggcgc cgctcgaatg cgacgtctgg
      181 agccacaagc agctcctgaa cgtcgtcagc cataccccgg tcgaggtcga ggcgctgacg
      241 cggctccagc agagcggcga gtgggtcgat ctcggccgcc gcttctttcc cgcgcccggc
      301 cggctccaca cctggtggag ctaccgctcg ccggattgga ccaggaacga ccgcggccga
      361 cggctcgacc atatgtgggc gacgcccgac gtcgccgcgc tcgcggtcgg ccacaaggtc
      421 cacgagccct gccgcaactg gacgcgtccg tcggaccacg tgccgctcgt caccgagttc
      481 gcggtgtgag cggaggccgc gacctcgccc gcgccatcga cgcgttgcgg cggggctggc
      541 cggtcgtcat cgacggctcc ggcttcctcg cggtcgagac ggccgacgat gccggcctcg
      601 ccgccttcga tgcgggcggg cccgccgacc tcctaatttc cggcaaccgg gcggtaacgc
      661 tgaagctcgc caaccagcgc gacgcggttc cgaccgggcc ggtacggatc gtccgctcgc
      721 cctggctcga ccttgccgag gcgaccgccg tcgccgatcc ggcgctcgac ctcaaaacgc
      781 cgctgaaggg gccgttccgc accatcccgc tcggctgcga caaggcggcg ctggcggcga
      841 tcaccctcac ccggcgggcc ggcctgctgc cggcgctgtt catcgacggc gacgcgggcg
      901 ccgacgccat ctcgatcgat gcggccgcgg tggccgcttt cgacaagtcg ccctcgctcg
      961 ccatcgcgtc gcgcgccaag ctgccgacgc gggccgcgga gcaggcggag atcgtcgcct
     1021 tccggtcgga ggaggatgcg gccgagcatg tcgccttgct gatcggccag ccgaacggcc
     1081 agccgccgct ggtgcggctg cacagcgaat gcctgaccgg ggacgtgctg gggtcgctca
     1141 aatgcgattg cgggccacag ctcgacgccg cgatcgcgga gatccggcgg gcgggctggg
     1201 ggatattgct ctatcttcgc caggaggggc gcggcatcgg cctcgtcaac aagctgcgcg
     1261 cttacgcgct ccaggaccag gggttcgaca cggttgacgc caaccaccgg ctcggcttcg
     1321 gcgacgacga gcgcgattat ggggtggcgg cgcggatgct ggcgaaattg tcgcagaccg
     1381 aggtcgcctt gctgaccaac aacccgcgca aggcggcggg gctgcgggcg gaagggatcg
     1441 acgtggtccg gcaagtgccg ctgaaggtcg gcgccaatgc gttcaatcgc gactatctcg
     1501 acaccaagcg ggcgcggagc gggcaccaat tgtgacgccg cggcgtcact cgcgctccag
     1561 ccgggcgagg atttcggcca cttcggcata atcgcgtgcg acgtgatgcg ctccggcggc
     1621 gcgcgcgcgg tcgccgtggc cggggccgat gtggccgccg gcgaggaggg cgatcacggt
     1681 catgccggcg gccgcgccgg cggcgacgcc gaccggatgg tcctcgatga ccaggcaccg
     1741 gggcggagcg atgccgagcc gctcggcgac gccgagatag atgtcggggt gaggcttgcc
     1801 gcgcgcgagg ccggcggcgc tgtagacgcg ctcctcgaaa tagtggccga ggccgagccg
     1861 ggccagcgaa gcgtcgagcc agtccttctc gctcgacgag gcgatggcga gcgagcggtg
     1921 cgcctgggcg gcgaggaatt tctggatacc ctcgatggct aggatgtccg ccacgcgggc
     1981 ggcgatcgcc tggtcgagca tgtcgccgag ttggtccgga tcgaaggaga ggccgctctc
     2041 ctcctcgatc cggcggtggc agtcgctcca gcgcaggccg gaataataag cgagggcgtg
     2101 gtcggcggtg acgggatggc cggcttcggt cagcaactcc gcgagcgcgg cgttggcgac
     2161 gacctcgctg tcgacgatga cgccgtcgaa atcgaagatg atcgccccga aggtcatgtc
     2221 agccgcgctc gccgaacaag gcggtgccga cgcggacatg ggtggcgccg atcgtcaccg
     2281 cggtctcgaa gtcggccgac atgcccatgc tgagcccggg cagcgcgtgc cggcgggcga
     2341 gcttggcgag caaggcgaaa taaggcgccg gttcgagctc ggccggcggg atgcacatca
     2401 agccacggac cgggaggccg gcctcgcggg ccgcatcgag cagggcggga agctccgcca
     2461 ccgcgcagcc gcctttttgc gcctcatcgc cgatattgac ctggatgaag cagtccggcc
     2521 gccgccccgc tttggccatg gcggcggcta gcgcggggat gagggatggg cggtcaagcg
     2581 agtggatcgc gtcgaacagc tcagcgacct cgtccgcttt gttcgactgg agccgcccga
     2641 tcaggtgcag ctccacgcct tcgtagcgct cgcgaagctc cggccatttc gcctgcgcct
     2701 cctggactcg attctcgccg aagatgcggt ggccggcctc gagcagcggc acgatggctt
     2761 cgggcgggtg ggtcttcgag accgcgatca gcgccacctc gccctgcgag cggccggcca
     2821 gcgccgccga gcgcgcgatc gcggcgcgca cctcccccaa tcttccggcg gctgatgcct
     2881 cgctcatgga cgctgctata gggagcgcta tgcaccgccg ccagcctctg ccccgcctct
     2941 ggctgatgac cgacgagcgg cagggggacg ctttgtgcag cgcgctcgag cggctgccgc
     3001 gaggctcggg cgtggtgttc cgccattacg gacttcccac cccggagcgt cgccggctgt
     3061 tcgggcaggt gcgggcggtg gcgcggcggc ggcggctggt gctgctgctg gccggaaccc
     3121 cgcgtctggc cgcggcgtgg ggcgccgatg gcagccatgg ccgcgggtcc catgccccgc
     3181 tgcgggggca ggtccgcacc gcgccagttc acgacgtgcg cgatatgatc gcggcggccc
     3241 gagcgggggc ggacctgctg ttcgtctcgc cggcgttcgg tacgcggtcg catccgggcg
     3301 cggcgccgct cgggcgggtg cggttcggcc tgctcgcgcg gcaggcgcgc gggccggtgg
     3361 tggcgctggg cggaatgacg ccgagccgct ttcgggggct ggcggccttc aggatctacg
     3421 gctgggctgc gatcgacgcg tggagcgccg gaccggctta gaagcggaaa gcggtgccga
     3481 cgtagaccgc ctggctgtcg cggcgctcgt cggccagcgg ctcgagccgc tcgcgctgga
     3541 tcttgtagcg gacgccgccg gtcacatcga cgttgcgcgc gatcgaatag gagccgccga
     3601 catcgagcga atagctgctt tcctgcggca tgatgcgcag ctgattgccg tcggtacgct
     3661 cggcgccgac cgcgacccgg ccggtgaact tcttgccgag atagctgacg ccgacctcgg
     3721 ctgcttcgcg gccgccgggg atcgggccgc cctggacctt ggcgacgtcg cccgaaagcg
     3781 cgaactgctt ccagccgacc gcgacgccca gattataggc ggtcggcgtg atcgccgtaa
     3841 ccggactggc ggaggtttcg gccgtcgccg ccgccgagcg gaccgcttcc gcggcggtgg
     3901 tcgcgcgggc gcggacggcg acgcggacgg ccttgctctt gtccggggaa gccgatgagg
     3961 gcgtgaagcg gaagctgttc gcctgcagac cgcggcgggc gaattcggcc gcgagccggg
     4021 gatcggcgac cgcgggcgtg aagccgctgg agccgaacga gggcgcggac cgcgccttct
     4081 tcgccttttg cggctggttc gaagcggcga atgctggggc cagcagcagg gatagagcga
     4141 caagcgacgc cccacccatt gcagcaatgc cacctagctt catcacgtct gtcgactccg
     4201 tcgtcgcccg tggatgtagt atgagtgcaa acccctgacc agcggactgg tgcccgttag
     4261 gcacgaatcg tttggcgttg ttgcacattc cccacaggaa gcttgcgccc cgatgaacaa
     4321 gcgggtcaac ggcggccccg gccgtcagat cttgccgctg atcgccagcg tcaggatcgg
     4381 cccgatatcg cggttcctgt cctcggtgaa cagaatcggc ccggtccgcc tgccgtcgtg
     4441 gaaatcgcgc gtcaactgca cattctggcc gatcaggttc tcgaccgttc cgcggacggt
     4501 gaggccgagc acgtccttat gctcgataaa ggcgctgagg atgccgggcg cgttcaggaa
     4561 ctggagccgc tggtcgagcc ggtagcccgc cgactggcga tattcgtcgt agccggcgcc
     4621 ccaggcccag ggcgtccccg gcacgtcgtg gcgcagattg gcgacgatgc gccgggtcat
     4681 ggtatcgttg atgccgcggt tccggcccgt caccggatcc tcgagcgacg aacgctggaa
     4741 ctggaggtcg aggtcgagct tggccccgcg ccagccaagc ggatcgaagt tgaaggtacc
     4801 ggtcatttgc acgccgtaga gcgtcgcgct gtcgagattg cccggcgact ggccgtcgtc
     4861 gccgaccggg atgacgtcga cgatgtcgct gatcaggcgg ccgtagaccc gcgccgtcgc
     4921 ggtgccccag cggccgaggt tgcgggtcgc ctcgacttcg gcgtcccagc tttgcggcgg
     4981 gacgaggttc gggttgccgg cattgacggt gccggcgctg acgttcgacg aggcgacgaa
     5041 gtcgaagaag ttcagctggc cgacgacccg ctcgagcttg gccgatacgt cgagccgcgg
     5101 cgaggctttc caggcgaccg aaatcaggcc cttgggccgc aggaaggtcc gtgccaaccc
     5161 gttctcgccg gactggctga gctgcgaata ttcgccgccg agcgaggcct gcagcgacag
     5221 aacggggctc agcggccgcc cgtagctcag catgacctcg ccgcgtttct cgtccacgcg
     5281 cgccgccgat ccgggcagcg gcaccggcac ccactggccg gccgcatcga gccgggcgag
     5341 gctgttggcg atatcgagta tgttgagcgc gccttccgcc gacacctgcc aatcggccgc
     5401 gccggcttgc cagcgatatt cggcgcgggc gatcgtctcg gcctcgtcgg cggtctgggt
     5461 gaacaaatcg ccggcgctcg cgcgttcgtc ggcgaagtcg atcgtcagcc gctgccggaa
     5521 gggcgtatgc tcgaaccgcc tcaatccgat cagcttcagc cggccgccgc cgaggccgaa
     5581 ctcatagtcg ccgccaagct catattccca tttgcggcgc cgctcctcca gccgccggtc
     5641 gcggtcgacg aggccgggga agctgcgcag cgagacctcg cgcagatcct ggtagtcgag
     5701 cgccaccgcc gcgttgaggt tgaggagcgt cccgccggcc tcgcgcctga ggctggcgcc
     5761 gagccgcggc acctcgccct cataggtcag ggtctcagcg cgccggtcgg tcaccgtccc
     5821 gtccggtgtg aacaccagct ccgggccttc ttcggcgccg atatagctct cattcgccat
     5881 gctcagcgta tagtcgaacc ccagcgccgc gccgctgacc gaggcttcgc cgttggtgag
     5941 ccggagcttg tcgaatttgg gccgatattg cgggcgccag gcgaagctgc cgctgacgcc
     6001 ggacgccgcg gtgacgatgt tggcgacttg gcccgacagg cccggcacgt cgagcgtcgc
     6061 gccgtcgacg atgtcgatgc ggacgacgtt ggcggcggcg atccggccga cctcggtcac
     6121 gacgtcgttc gacttgcccg agaatcggct gccgttgatc agcacgttgg tggcggcctg
     6181 gccgagcccg cgccgctcgt cgtcgccttc ctggatggtg aagcccggca cctggcgcag
     6241 catgtccagc gccgtcttgg ggctgaagcg ggcgaaatcg gccggggcat aactccttgc
     6301 cccctcggcg gcggcgggaa gcgccggcgc agcctccgcc ggcacctgcg cctgggctgc
     6361 ggctgtgccg gccggtgcgc cgagcgtcac ggcgaccagc gccgccagcc tcggtccgtt
     6421 caggatattc gtcatccgct ccccttcgct gccgccgcgg ggccaagcgc atcgaagacc
     6481 cgcacgcagc aagacttcga tgagcctcat cgggcgaccg accaacggca cggcgcctcg
     6541 catcggcccg tccgccggaa ctggcgcctt cgctttcggt tgttcggaca aggagattcg
     6601 cgatgagcaa gaacaaccac aaccacaacg ccatgcaatt cacccagaac cagaataacg
     6661 agatgtcgga cgatccgggc gacggcagcc gcaccagctt cacgctcggc aaggacggca
     6721 ccagcccggt cgagaagaag cctcccaacg acgcgcagga ccgatcgacc atcgaagcgt
     6781 tcggcgagga aggcgcgggc atcgccgcca aggagtaagc gccatgtccg acaccgaaac
     6841 cctcgagatg atccacgagg atcagctgcc cgggcatgaa tcgcagctcg agcccaagcc
     6901 cgattgggag ccgcgctacc cgggctcggg ccggctcgtg
//
LOCUS       DASVRK010000220         7600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_232941, whole genome shotgun sequence.
ACCESSION   DASVRK010000220 DASVRK010000000
VERSION     DASVRK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232941"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1414)
                     /locus_tag="VF589_08680"
     CDS             complement(101..1414)
                     /locus_tag="VF589_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8257697.1"
                     /translation="MIYPAGRLILAAAAIAPLALVIGVAVPVYWFAGLALLVFLLAMA
                     GVDALAGARPADVDIVYEGPRAVTVGETFEVAIDASFRGASPAAAQAALSADPLLEAP
                     QGYRRPVAIAGGAGQASLPFTATRRGIARLSGVWLRWRGPLGLVWKQRRLTLDQEIIV
                     TPDIRPVREKSAQMLHRDAAHGLTPQLQVGEGAEFEALTEYRQGMDRRSIDWKQSARH
                     TKLMAKEFRTERNNNVILAIDTGRVMCEPLAGVPRVDRAVAAALLTAFVALKDGDRVG
                     LFGFDSHPRVTSKAVAGARSFALLQRLAAGIDYSTAETNYTLGLATLATGLNRRSLIV
                     IFTEFADTTSAELMLNAVGALLKRHLVLFVVMRDQELEDYAGAEPREPEDVTRAVTAA
                     ALLRERRLVMSRLRHLGVHVLEAGHDQSGPALVNAYLDLKRRDVL"
     gene            complement(1411..2367)
                     /locus_tag="VF589_08685"
     CDS             complement(1411..2367)
                     /locus_tag="VF589_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8257698.1"
                     /translation="MNVTDVKSLADGIRSEIGRAIVGQDEAVELMLIALFSSGHILLE
                     GPPGTAKTFLAQCFARALGLDYGRIQFTPDLMPGDIVGSNLFNFQTSQFTLTRGPIFC
                     ELLLADEINRTPPKTQAALLEAMQERSVTIDGATHRLSDRFMVIATQNPIEQQGVYPL
                     PEAQLDRFLFKHVMAYPSLEEERRIVAVHGARFGAPRPGEWGVETRVGAAEVAAAIAG
                     VAAVPMVEEVVDYVVSLIRATRDSAEIASGASPRAAAMLAGAARARAALEGRDYVIPD
                     DVKALAPAVLRHRLILSPAAEIEGRPVDQVVARLLDQVEAPR"
     gene            complement(2364..3569)
                     /locus_tag="VF589_08690"
     CDS             complement(2364..3569)
                     /locus_tag="VF589_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX8257699.1"
                     /translation="MTASATGETFDVKVVIGLIGAGVIAFAAFLLLAAYADDFRSGLD
                     GRPHPLSLSATGFKGLTQLLEATGARPRLIRSGDGLDTEDLVVAALDETLAEGALAAL
                     TDRRESQATLLILPKWSTAPDELHPGWVRRIGPHSPGDVAELLEGVADVTVSHDEVPA
                     GGAAVGGNYLEGVRVPAPALSQTIAGEDLTPLLTHAGGGILLARLGEGPLYILADPDL
                     MSNHGLRDARTARAALDILALLNSTGAEGIAFDLTLNGFGRKPSLLKLLLEPPFLVLT
                     LALAAAALLAGLHGAFRFGPERREERVIALGKGALVENSAGLIRSARREHRAGGAYAE
                     LVRETAAREAGAAGLTGAELDAYLDRLSRAGGPRFSALAARIAAARDRDELVSAARAL
                     FQWKKDISS"
     gene            complement(3569..4198)
                     /locus_tag="VF589_08695"
     CDS             complement(3569..4198)
                     /locus_tag="VF589_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX8257700.1"
                     /translation="MANETAEAETGSEFAAAHRDLVADESIQFAFSDFKPPEVPAWLK
                     WLGEFLYEIFPLLKILFWAGVAAAVIAILYVVARRLSGSDWPWRRRRDDEPAPATDWR
                     PEEAPARALLGEADALAARGRFSEAAHLLLFRSIEEIDARRPRLVRPALTSRDIAGEP
                     AIPPGPRQAFASIVMTVERSLFGGRPLGEPDWRQCRAAYEEFAFAGAWR"
     gene            4354..5091
                     /locus_tag="VF589_08700"
     CDS             4354..5091
                     /locus_tag="VF589_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257701.1"
                     /translation="MDAKIDMGRVVSRGFQVIGRHALPFIGLALLLGVLPTFLTYRFL
                     IGNLEIGAAMFLSPFYWLSTLISLICTYLLQATLVRSSILELSGRGPDVGGSLAMAVK
                     LLLPMIGIAILTTMIVGIGMMLLVVPGIIAYIMLIVAVPVLVEERNGVIGSMQRSRAL
                     TKGSRWRILVLLLLFGIIYFAVSMLATFALGAIGIGSALDAMAVVQSLTGGIVALIVG
                     AMLASLYVELRTVKEGATPDGLADIFA"
     gene            complement(5117..5914)
                     /gene="trpA"
                     /locus_tag="VF589_08705"
     CDS             complement(5117..5914)
                     /gene="trpA"
                     /locus_tag="VF589_08705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX8257702.1"
                     /translation="MNDRYGVLFERLRARVEGAFGAFVMLGDPDLETSAAILDALVEG
                     GADMIEVGIPFSDPVADGPTIQAAAERALARGTTPADCFALLAAFRARHPHVPVGVLT
                     YANIVLARGRPAFYRASAAAGVDSVLVADVPAFEADPFVAAARAEGVAPVMIAATNTP
                     PPTLRRLAELGQGYTYCVARAGVTGAGAEMRLDHGDLFAALAECGAPPPVLGFGISTP
                     AHVRAALASGAAGVISGSAIVKLIEGGAASLPAVRTFVAAMKAATAG"
     gene            complement(5911..7086)
                     /gene="trpB"
                     /locus_tag="VF589_08710"
     CDS             complement(5911..7086)
                     /gene="trpB"
                     /locus_tag="VF589_08710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX8257703.1"
                     /translation="MLMSGRFGAYGGAYVPEILMPALEELEASFLAAQQDADFQAELA
                     ELLSKYAGRPTPLTRCRNLGSDRARIYLKREDLLHGGAHKTNQVLGQALLARRMGKTR
                     IIAETGAGQHGVATALAGALFGLETRIYMGAHDVERQRLNVFRMQLMGAEVVPVESGG
                     RTLKDAVNEALRDWTASFADTHYLLGTVAGPHPFPLMVREFQRVIGKEARAQILAEEG
                     RLPDAVIACVGGGSNAMGAFADFVDDEDVELIGVEAAGRGLDGAEHGATLLRGRPGVL
                     HGAETYVLQDADGQIADSWSISAGLDYPAVGPEHAHLKESGRAEYVGCTDQDALDAFA
                     LLARSEGIVCAFESAHALAHALKLADAATEDTLLLVNLSGRGDKDMEQAQRLLKFGA"
     gene            complement(7196..>7600)
                     /locus_tag="VF589_08715"
     CDS             complement(7196..>7600)
                     /locus_tag="VF589_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HEX8257704.1"
                     /translation="VQLHGEEDAGYIKALKALLPEGCEIWAAGAVGRDVPEPRIGSDR
                     TLFDTKVGGRSGGTGMAFDWSRIARRRDLGQSLLAGGLKPANARAASQVGAFALDVGS
                     GVEMAPGRKDAAKLHAFFEALRRPVRGDVQTC"
BASE COUNT         1128 a         2639 c         2735 g         1098 t
ORIGIN      
        1 accgatttgc gctcggcgcg gaccagcagc gcctcgagcc gccgccactc gtcctcgcgc
       61 gcggccctga agcgccggga atcggccgaa ggcagcgcgc tcacagcaca tccctccgct
      121 tgaggtcgag ataggcgttg accaaagccg gccccgattg gtcgtgcccg gcttcgagga
      181 cgtggacgcc gaggtggcgc agccggctca tcaccagccg ccgctcgcgc agcagcgcgg
      241 ccgcggtgac ggcgcgggtg acgtcctcgg gctcgcgcgg ctcggcgccg gcataatctt
      301 ccagctcctg gtcgcgcatg accacgaaca ggacgaggtg gcgcttcagc agcgcgccga
      361 ccgcgttcag catcagctcg gcgctggtcg tgtccgcgaa ttcggtgaag atcacgatca
      421 gcgaccggcg gttgagcccg gtcgcgagcg tcgccaggcc gagcgtgtaa ttggtttccg
      481 ccgtcgaata gtcgatcccg gcggcgagcc gctggagcaa ggcgaaggag cgcgccccgg
      541 cgaccgcctt gctcgtcacg cgcgggtgtg aatcgaaacc gaacagcccg acccggtcgc
      601 cgtccttcag cgccacgaag gcggtgagca gggcggcggc gaccgcgcgg tcgacccgcg
      661 gcacaccggc cagcggctcg cacatcaccc gccccgtatc gatcgccagg atcacgttgt
      721 tgttgcgctc cgtacggaat tccttggcca tcagcttggt gtgccgggcc gactgcttcc
      781 agtcgatgct gcggcggtcc atgccctggc gatattcggt cagcgcctcg aactccgcgc
      841 cctcgccgac ctgcaattgc ggcgtgagcc cgtgcgcggc gtcgcggtgg agcatttgcg
      901 cgctcttctc gcgcaccggg cggatgtcgg gcgtgacgat tatctcctgg tcgagcgtca
      961 gccggcgctg cttccagacc agcccgagcg gcccgcgcca gcgcagccag acgccgctca
     1021 gccgcgctat gccgcgccgc gtcgccgtaa acggcagcga ggcctggccg gcgccgcccg
     1081 ctatcgcgac cggccggcga tagccttgcg gcgcctccag cagcgggtcg gcggagagcg
     1141 ccgcctgggc cgcggcgggc gaggcgcccc ggaagctcgc gtcgatcgcc acctcgaaag
     1201 tctcgccgac ggtgacggcg cgagggcctt cgtagacgat atccacatct gccgggcgcg
     1261 cgccggcgag cgcgtcgacc cccgccatgg cgagcaggaa cacgagcagg gcgaggccgg
     1321 cgaaccaata aacgggcacg gccacgccga tcaccagcgc gagcggggcg atcgccgctg
     1381 cggcgaggat caggcggccg gcgggataga tcaacgcggc gcctcgacct ggtcgagcaa
     1441 gcgggcgacg acctggtcga ccggccggcc ctcgatctcg gcggccggcg agaggatcag
     1501 gcggtggcgc agcaccgccg gggcgagcgc cttgacgtcg tcggggatga catagtcgcg
     1561 cccctccagc gccgcccgcg cgcgcgccgc cccggccagc atggcggcgg cgcgcgggct
     1621 ggcgccgctc gcaatctccg ccgaatcgcg ggtcgcgcgg atcagggaaa cgacatagtc
     1681 gacgacttct tccaccatcg gcaccgccgc caccccggcg atggcggcgg ccacctcggc
     1741 cgcgccgacc cgggtctcga cgccccattc gccggggcgc ggcgcgccga agcgggcgcc
     1801 gtggacggcg acgatccttc tttcctcctc cagcgaggga taggccatca catgcttgaa
     1861 caggaaacgg tcgagctgcg cctcgggcaa tggatagacg ccctgctgct cgatcggatt
     1921 ctgggtcgcg atcaccatga agcggtcgga cagccggtgg gtggcgccgt cgatcgtcac
     1981 cgaccgctcc tgcatcgcct cgagcagggc cgcctgggtc ttgggcgggg tgcggttgat
     2041 ctcgtcggcg agcagcagct cgcagaagat cgggccgcgg gtgagcgtga actggctggt
     2101 ctggaagttg aacaggttcg atccgacgat gtcgccgggc atcaggtccg gcgtgaactg
     2161 gatgcggcca taatcgaggc ccagcgcgcg ggcgaagcat tgggcgagga aggtcttggc
     2221 ggtgcccggc ggaccttcga gcaggatgtg gccggaggag aataaggcga tcagcatcag
     2281 ctcgaccgcc tcgtcctggc cgacgatggc gcggccgatc tcgcttctta tgccgtcggc
     2341 gagcgatttg acgtcggtta cattcacgag ctgatgtcct tcttccactg aaacaatgcg
     2401 cgggcggcgc tgacgagttc gtcgcggtcg cgcgcggcgg cgatgcgggc ggcgagcgcg
     2461 ctgaaccggg gcccgcccgc gcgcgacagg cggtcgagat aggcgtcgag ctcggccccc
     2521 gtcaggccgg cggctccggc ctcgcgggcg gcggtttccc ggaccagctc ggcataggcg
     2581 ccgccggcgc gatgctcgcg ccgcgcgctg cggatcaggc cggcgctatt ctcgaccagc
     2641 gcgcccttgc cgagcgcgat cacgcgctct tcgcgccgct ccggcccgaa ccggaaggcg
     2701 ccgtgcaggc cggcgagcag ggccgcggcg gcgagggcga gcgtgagcac gaggaagggc
     2761 ggctccaaca ggagcttgag gaggctcggc ttgcggccga agccgttgag ggtgagatcg
     2821 aaggcgatgc cttcggcgcc ggtcgagttg agcagcgcca ggatatcgag cgcggcgcgg
     2881 gcggtccgag cgtcgcggag gccgtgattg ctcatcaggt ccgggtcggc gagaatgtag
     2941 agcggaccct cgccgagacg ggcaagcaat atcccgccgc cggcatgggt cagcagcggc
     3001 gtcaggtcct cgcccgcgat cgtctggctg agcgccggtg ccggcacccg cacgccctcc
     3061 agatagttcc ccccgaccgc ggcgcctccg gccggcacct cgtcgtggga tacggtcacg
     3121 tcggccacgc cttcgagcaa ctcggcgacg tcccccggag agtgcggtcc gatccgcctc
     3181 acccagccag gatggagctc gtccggagcc gtcgaccatt tgggaaggat cagcaaagtg
     3241 gcctggcttt cgcggcggtc ggtgagcgcc gccagcgcgc cttccgcgag cgtttcgtcg
     3301 agcgccgcga ccaccagatc ctccgtgtca aggccgtcgc ccgaccggat cagccgcggc
     3361 cgggcgccgg tcgcctcgag caattgggtc agccctttga agccggtcgc ggagagcgag
     3421 agcgggtgcg ggcgcccgtc gagtccgctg cgaaagtcgt cggcgtaggc cgccagcagc
     3481 aagaaggcgg cgaaggcgat cacgccggcg ccgatcaggc cgatcaccac cttgacgtcg
     3541 aacgtctcgc cggttgcgct cgcggtcatc agcgccaggc tccggcgaag gcgaattcct
     3601 cataagcggc gcggcactga cgccagtcgg gctcgccgag cggacgcccg ccgaacaggc
     3661 tgcgctcgac cgtcatcacg atcgaagcga acgcctgccg cggccccggc gggattgccg
     3721 gctcgccggc aatgtcgcgg ctggtgagcg ccggccgcac cagcctgggc cggcgggcgt
     3781 cgatttcctc gatgctccgg aacaggagga ggtgggccgc ttcggagaaa cggccgcgcg
     3841 cggcgagcgc gtccgcctcc ccaagcagcg cccgcgccgg cgcctcctcg ggccgccagt
     3901 cggtcgcggg ggcgggctcg tcgtcccggc gccggcgcca gggccagtcg gagccggaga
     3961 ggcgccgggc gaccacgtag agtatcgcga tgacggcggc ggcgacgccg gcccaaaaca
     4021 ggatcttcag cagcgggaag atctcgtaca ggaactcgcc gagccacttg agccaggccg
     4081 gcacttcggg cggcttgaag tcggagaagg cgaattggat cgactcgtcc gcgacgaggt
     4141 cgcgatgcgc ggccgcgaat tcggagcccg tctccgcttc agccgtctcg ttcgccacgc
     4201 catcccctcg cgccgttccg ccggccggcc gcatctaggc gggcccgcgc gagcggcgca
     4261 aggacttcgc atttccggct ggacagagcg gccgcaatcg gaaagcttgg gggctcggca
     4321 acaggggagg cggatatggc cacggcggcg gcaatggatg caaagatcga catgggcagg
     4381 gtcgtctcgc gcggctttca ggtgatcggg cggcacgcgc tccccttcat cgggctcgcg
     4441 ctgctgctcg gcgtgctgcc gaccttcctc acctaccgtt ttctgatcgg caacctcgag
     4501 atcggggcgg cgatgttcct gagccccttt tactggctga gcacgctgat atcgttgatc
     4561 tgcacctatc tgctccaggc gacgctggta cgctcgtcga tccttgagct cagcgggcgc
     4621 ggtccggacg tcggcggcag cctggcgatg gccgtcaagc tgctcctgcc gatgatcggc
     4681 atcgccatcc tcacgaccat gatcgtgggt atcggcatga tgctgctcgt cgttccgggc
     4741 atcatcgcct acatcatgct gatcgtggcc gtcccggtgc tggtcgagga gcgcaacggc
     4801 gtcattggga gcatgcagcg cagccgcgcg ctgaccaagg ggtcgaggtg gcggatctta
     4861 gtcctcctcc tgcttttcgg tatcatttat ttcgcggtct cgatgctggc tacgttcgcc
     4921 ctcggtgcga tcgggatcgg cagcgccctc gacgcgatgg cggtggtgca gtcgctgacc
     4981 ggcggcatcg tggcgctgat cgtcggggcc atgctggcat cgctctatgt cgagctgcgc
     5041 accgtcaagg aaggcgccac gccggacggc ctcgccgaca ttttcgccta gctcagggcg
     5101 cgagggggcg gccgggtcag ccggcggtcg ccgccttcat ggccgcgacg aaggtgcgga
     5161 cggcgggcag cgacgcggcg ccgccctcga tcaatttgac gatcgccgaa cccgaaatca
     5221 ccccggccgc gccggaggcg agcgccgcgc ggacatgggc gggggtcgaa atgccgaagc
     5281 cgagcaccgg cggcggcgcc ccgcattcgg ccaaagcggc gaacaggtcg ccatggtcga
     5341 gccgcatctc ggccccggcg ccggtcacgc cggcgcgggc gacgcaatag gtatagccct
     5401 ggccgagttc ggccagccgg cgcagggtcg gcggcggcgt attggtggcg gcgatcatca
     5461 ccggcgccac cccttcggcg cgggcggcgg cgacgaaagg gtcggcttcg aatgccggaa
     5521 cgtcggcgac cagcacgctg tccaccccgg ccgccgccga ggcgcggtag aaagccgggc
     5581 gtccccgcgc cagcacgatg ttggcgtaag tgagcactcc caccggcacg tgcgggtggc
     5641 gcgcgcggaa ggcggcgagc agggcgaagc agtcggccgg ggtggtcccc cgcgccagcg
     5701 cccgctccgc cgccgcctgg atggtcgggc cgtcggcgac cggatcggaa aaggggatgc
     5761 cgacctcgat catgtcggcg ccgccctcca ccagcgcgtc gaggatggcg gcgctcgtct
     5821 ccagatccgg gtcgccgagc atcacgaaag cgccgaacgc gccctcgacg cgcgcgcgaa
     5881 gccgctcgaa caggacgccg tagcggtcgt tcacgcgccg aacttcagga gccgctgcgc
     5941 ttgctccata tccttgtcgc cgcggcccga gaggttgacc agcagcaagg tgtcctcggt
     6001 ggcggcatcg gcgagcttca gcgcatgggc cagcgcatgg gcggactcaa aggcgcagac
     6061 gatcccttcg gagcgcgcca ggagggcgaa ggcgtcgagc gcgtcctggt cggtgcagcc
     6121 gacatattcg gcgcggccgc tttccttgag gtgcgcatgc tcgggcccga ccgcgggata
     6181 atcgagcccg gccgagatcg accagctgtc ggcgatctgc ccgtcggcat cctgcagcac
     6241 ataggtttcg gcgccgtgca gcactcccgg ccgcccgcgc agcaaggtgg cgccatgctc
     6301 ggcgccgtcg agcccgcgcc cggccgcctc gacgccgatc aattccacgt cttcgtcgtc
     6361 gacgaagtcg gcgaaggcgc ccatcgcgtt ggagccgccg ccgacgcagg cgatcaccgc
     6421 gtcgggcagg cgcccttcct ccgccaatat ctgcgcgcgc gcctccttgc cgatcacgcg
     6481 ctggaactca cgcaccatca gcgggaaggg atgcgggccg gccacggtgc cgagcagata
     6541 atgggtgtcg gcgaagctcg ccgtccagtc gcgcagcgcc tcgttgaccg cgtccttgag
     6601 cgtccggccg ccgctctcca ccggcaccac ttcggcgccc atcagctgca tgcggaacac
     6661 gttcagccgc tgccgctcga cgtcatgcgc gcccatgtag atgcgggtct cgaggccgaa
     6721 caaggcgccc gccagcgcgg tggcgacgcc atgctggccg gcgccggtct cggcgatgat
     6781 ccgggtcttg cccatgcgcc tggcgagcag cgcctggccc agcacctggt tggtcttgtg
     6841 ggcgccgccg tggagcaggt cctcgcgttt caaatagatg cgcgcgcggt cgctgccgag
     6901 gttgcggcag cgcgtcagcg gcgtcggccg gccggcatat ttggacagca gttcggccag
     6961 ctcagcctgg aaatcggcat cctgctgcgc ggcgaggaag ctcgcctcga gctcctccag
     7021 cgccggcatc aggatttcgg ggacataggc gccgccataa gcgccgaagc ggccgctcat
     7081 cagcatgacc gctcccccaa gccgtcaccc ccgcggaagc gggggtcccg cttcgcgtgt
     7141 ggtgtggaag aagaagaagt gggattcccg ctttcgcggg aataacgaag agggctcaac
     7201 atgtctggac atccccgcgc acggggcggc gcaaggcttc gaagaaggcg tggagtttgg
     7261 cggcgtcctt gcggccgggc gccatctcga cgcccgagcc gacgtcgagc gcgaaggcgc
     7321 cgacctggct ggcggcgcgg gcattggcgg gcttgaggcc gccggcgagc aggctttggc
     7381 caaggtcgcg gcggcgggcg atgcgcgacc agtcgaacgc catgccggtc ccgcccgatc
     7441 ggccgccgac cttggtgtcg aacaaggtcc ggtcggagcc gatgcgcggc tccggcacgt
     7501 cgcggccgac cgcgccggcc gcccagattt cgcagccttc ggggagcagc gccttcagcg
     7561 ccttgatgta gccggcatcc tcctcgccgt ggagctggac
//
LOCUS       DASVRK010000221         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_418475, whole genome shotgun sequence.
ACCESSION   DASVRK010000221 DASVRK010000000
VERSION     DASVRK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418475"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="ispG"
                     /locus_tag="VF589_08720"
                     /gene_synonym="gcpE"
     CDS             complement(<1..786)
                     /gene="ispG"
                     /locus_tag="VF589_08720"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX8257705.1"
                     /translation="MSIRPWRDIARRPSRQIMVGNVPVGGDAPVTVQTMTNTSTADAR
                     ATIDQIRRCEEAGADIVRVSCPDVESTAALKQIVRAANVPIVADIHFHYKRALEAADA
                     GAACLRINPGNIGSADRVREVVNAAKANGCAIRIGVNAGSLERDLLEKYGEPCPEALV
                     ESALDHIRLLEDHDFRDYKVAVKASDVFLAVAAYQQLAEAVDCPLHLGITEAGGLRGG
                     TVKSAIGIGSLLWFGIGDTIRVSLSAEPEEEVRVGFEILKSLGI"
     gene            894..1826
                     /locus_tag="VF589_08725"
     CDS             894..1826
                     /locus_tag="VF589_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8257706.1"
                     /translation="MNDDPRKAGWKLVIHGGAGILERHLISPADEADVRAALNHALGT
                     GAELLEGGGSALDAVEAAVRELEDDPHFNSGRGAVFTFQGTNELDAAIMDGRTRAAGA
                     VAGVSGTRNPVSLARAVMENSPHVMLSGRGADQYSREQGLEQVEPEYFAVPERRRQLE
                     EMKSRGDWFDADMKYGTVGAVALDRHGHVAAATSTGGITGKRWGRIGDSPLIGAGTYA
                     DDRAGAVSATGSGEFFIREAVGHEICARIRFLGDTAQAAADTVMAEVGELGGTGGVIV
                     VTPRGEMVFSFNSVGMYRGMASAEGATVAIFGDE"
     gene            complement(1925..2455)
                     /gene="pal"
                     /locus_tag="VF589_08730"
     CDS             complement(1925..2455)
                     /gene="pal"
                     /locus_tag="VF589_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX8257707.1"
                     /translation="MYRNGFKLAALAAALALGACAKRAPDQLPPPPSDGGGGMDAGAG
                     DTSGAGMDGTAVPGSQAHFVQNVSSDRVLFDTDSHTLDDRSRAILDTQAAWLQQYPNV
                     GVSIEGHADERGTREYNLALGDRRANAAKNYLAARGVSAARMTTISWGKERPAAEGSD
                     PSAWAQNRRAVTVLPQ"
     gene            complement(2484..>3015)
                     /gene="tolB"
                     /locus_tag="VF589_08735"
     CDS             complement(2484..>3015)
                     /gene="tolB"
                     /locus_tag="VF589_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074574.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEX8257708.1"
                     /translation="YILYSMAVGGNTDIYRVSVNGGPSQRLTSAPGIDVGGSYSPDGR
                     RIVFESDRSGRQQLYVMNADGSDQRRISFGGGGYATPEWSPRGDLIAFTKLAGNFRVG
                     VMTPEGGGERLLTNSWQDEAPTWSPNGRVIQFFRTSQGRGGSSSVWQVDLTGVNERRI
                     PTPLSGSDPAWGPLRQ"
BASE COUNT          459 a         1025 c         1059 g          472 t
ORIGIN      
        1 gatgcccagg ctcttgagga tttcgaagcc gacccggacc tcctcctccg gctcggcgga
       61 gagcgagacc cggatggtgt cgccgatgcc gaaccaaagc agggagccga tcccgatcgc
      121 gctcttgacg gtgccgccgc gaaggccgcc ggcctcggtg atgccgaggt ggagcgggca
      181 atcgaccgct tcggcaagct gctgataggc ggcgacggcg aggaagacgt ccgacgcctt
      241 caccgccacc ttatagtcgc ggaaatcatg gtcctcgagc agccggatat gatcgagcgc
      301 gctctcgacg agggcttccg ggcagggctc gccatatttt tcgaggaggt cgcgctccag
      361 gctgccggca ttgacgccga tccggatcgc gcagccattg gccttggccg cgttcaccac
      421 ctcgcgcacc cggtcggccg agccgatatt gccagggttg atgcgcaagc aggccgcgcc
      481 ggcatcggcg gcctcgagcg cgcgcttata gtgaaagtgg atgtcggcga cgatcggcac
      541 attcgccgcc cgcacgatct gcttcagcgc cgccgtcgat tcgacgtcgg ggcaggagac
      601 ccggacgatg tccgccccgg cttcctcgca gcgccggatc tggtcgatcg tcgccctggc
      661 gtcggcggtg gacgtgttgg tcatggtctg gaccgtcacc ggcgcgtcgc cgccgaccgg
      721 aacgttgccg accatgatct ggcgcgaagg gcggcgcgca atgtcgcgcc agggacggat
      781 ggacatgggc accctataga cgcttgagat tacaaggcca aggcggccgg cttgctctgg
      841 tcaaccaccc ttattctgct atgcggcctg ccgaccgtca ctggagctgc cgaatgaatg
      901 acgaccctcg caaggccggc tggaagctcg tcatccatgg gggagccggc atcctcgagc
      961 ggcacctgat ttcaccggcg gacgaggcgg acgtccgcgc ggcgctcaac catgcactcg
     1021 ggaccggagc ggagttgctc gaaggcggcg ggtcggccct cgacgcggtc gaagcggcgg
     1081 tgcgggagct cgaggacgat ccgcacttca actccggccg gggcgccgtc ttcaccttcc
     1141 agggcaccaa cgagctcgac gccgcgatca tggacggccg cacgcgcgcg gccggcgcgg
     1201 tcgccggcgt cagcggaacc cgcaacccgg tgagcctcgc ccgcgcggtg atggagaaca
     1261 gcccgcacgt gatgctgagc ggccgcggcg ccgaccaata ttcgcgcgag caggggctgg
     1321 agcaggttga gccggaatat ttcgccgtcc ccgaacgccg gcgccagctg gaggagatga
     1381 agagccgcgg cgactggttc gacgccgaca tgaaatacgg cacggtcggc gcggtcgcgc
     1441 tcgaccggca cggccatgtc gccgccgcga cctcgaccgg cggcatcacc ggcaagcgct
     1501 gggggaggat cggcgattcg ccgctgatcg gcgccggcac ctatgccgac gaccgcgccg
     1561 gcgccgtgtc ggcgaccgga tcgggcgaat ttttcattcg cgaagccgtc ggccacgaaa
     1621 tctgcgcccg catccgtttt ctcggcgaca ccgcgcaggc cgccgcggac acggtcatgg
     1681 cggaggtcgg cgagctcggc ggcaccggtg gggtgatcgt ggtcacgccg cgcggcgaga
     1741 tggtgttctc gttcaattcg gtgggcatgt accgcggcat ggcgtcggcc gaaggcgcga
     1801 cggtcgccat tttcggcgac gaatagccac ctgtcgttcc ccgggagccg ttccccggcg
     1861 aaggccgggg tccagcccta ccagccctac cagcctgggc cccggccttc gccggggcac
     1921 gacttcattg cggcagcacc gtcaccgcgc gccgattctg ggcccaggcg ctcggatcgg
     1981 agccttccgc ggccggccgc tccttgcccc agctgatcgt cgtcatccgg gcggccgaaa
     2041 ccccgcgcgc ggcaaggtaa ttcttggcgg cgttggcgcg ccggtcgccg agcgccagat
     2101 tatattcgcg cgtgccgcgc tcgtcggcat ggccctcgat cgagacgccg acattgggat
     2161 attgctgaag ccacgccgcc tgcgtgtcga ggatcgcgcg ggagcggtcg tcgagcgtgt
     2221 ggctgtcggt gtcgaacagc acccggtcgg agctgacatt ctggacgaaa tgcgcctggc
     2281 tgccgggcac tgcggtgccg tccatgcccg caccgctcgt gtcgccggcg ccggcatcca
     2341 tgccgcctcc gccgtccgac ggcggcggcg gcagctggtc cggcgcgcgt ttggcgcagg
     2401 cgccgagcgc gagcgccgcg gccagcgccg caagcttgaa gccgtttctg tacatgttca
     2461 cctcctgata cgcatgggat agatcactgg cgcaatgggc cccacgccgg gtcggagccg
     2521 ctgagcgggg tcggtatgcg gcgctcgttg acgccggtaa ggtcgacctg ccagacgctc
     2581 gacgagccgc cgcggccctg gctggtgcgg aagaactgga taacgcggcc gttgggcgac
     2641 caggttggcg cctcgtcctg ccagctgttg gtgagcagcc gctcgccgcc gccctcgggc
     2701 gtcatcactc ctacccggaa attgccggcg agcttggtga aggcgataag gtcgccgcgc
     2761 gggctccatt cgggagtcgc atagccgccg ccgccgaagc tgatccgccg ctgatccgat
     2821 ccgtcggcgt tcatcacgta gagctgctgc cggcccgagc gatcgctttc gaacacgatc
     2881 cgccggccgt cgggggaata gctgccgccc acgtcgatgc cgggcgcact ggtgagccgc
     2941 tgcgaaggcc cgccgttcac ggaaacccgg tagatgtcgg tgttgccgcc gaccgccatc
     3001 gaatagagga tgtag
//
LOCUS       DASVRK010000222        10115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_41114, whole genome shotgun sequence.
ACCESSION   DASVRK010000222 DASVRK010000000
VERSION     DASVRK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41114"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /gene="greA"
                     /locus_tag="VF589_08740"
     CDS             <1..317
                     /gene="greA"
                     /locus_tag="VF589_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8257709.1"
                     /translation="ERQGQVEAQIADIDDRLSRAMVIDPTTLSGDKVVFGATVHLLDE
                     NDKPVKYQIVGQTEADAKIGRISYSSPLGRALIGRSVGEEVEVSAPSGDKYYSIEKVE
                     FI"
     gene            375..1016
                     /locus_tag="VF589_08745"
     CDS             375..1016
                     /locus_tag="VF589_08745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8257710.1"
                     /translation="MRPPDSWKRARLTLGIAGVAAFAWLVISTAGLGDVAMMWAGFIP
                     ARVGGAGGAEAMAPLALTPLTATLVHIGFLHLALNLVILLVCGRPVENILGWQAILIL
                     FLAGAYAAAAAHYGLDPSDMSPMVGVSGSVSAVLGAYAMLFSRFKVKVANPTLGLWLN
                     ILWLGAAWILFQLAVGFTFERPGAPTGVAAHIGGFLAGLVLAKPLLLFRYRRA"
     gene            1907..2176
                     /locus_tag="VF589_08750"
     CDS             1907..2176
                     /locus_tag="VF589_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257711.1"
                     /translation="MELAIAVVIFMVGLGLWFWAISLLTKLHGLALAITGVIVTIVTL
                     AITIALVRQGEEHIYAALAFMLSGIAALAIITLSCAKAGSTEERE"
     gene            complement(2447..2689)
                     /locus_tag="VF589_08755"
     CDS             complement(2447..2689)
                     /locus_tag="VF589_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX8257712.1"
                     /translation="MSNKQSKMHLVFGGRVRDPQGLDFVDLEAVDLVGLYPDYASALE
                     AWRSNAQRTVDDAEMKYVVVHLHRLLEPDLDGQPQP"
     gene            complement(2959..3534)
                     /locus_tag="VF589_08760"
     CDS             complement(2959..3534)
                     /locus_tag="VF589_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8257713.1"
                     /translation="MIKFGWAAAGIAAASLAAPAPAQVQNQDPGRFIQSLAGTGLGAL
                     RGEKSAARGKFRSLLAQHFAVEAIGDRLIRRWRPKISQAQYAAYKAALPGFIVGTYAD
                     RLHAYADASVKVVRVQSQGNNAAVLTQIANPGSRPVSAIWSVTKVGGGYKVTNLVVSG
                     VNVTMAQTADFDSYVQRNGFDALVEFMRRRG"
     gene            complement(3653..4165)
                     /locus_tag="VF589_08765"
     CDS             complement(3653..4165)
                     /locus_tag="VF589_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257714.1"
                     /translation="MSITDTANDTGTTTNNGADGGGRVSAVTGKARDKASAAYSAARE
                     RTGSAYGTVRQGASQAKSRTADGIETNPVGALIGGLAIGALVAAMLPKSRREAELLGD
                     VGRRINDTAREAARAATEAGKGKLDELGLNRDTAKQKLSDIAGGAGEALKTSAGAAAQ
                     TVKSGQQQPQ"
     gene            complement(4162..4563)
                     /locus_tag="VF589_08770"
     CDS             complement(4162..4563)
                     /locus_tag="VF589_08770"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX8257715.1"
                     /translation="MKGPTPEAELTRAQAEVEAAKKRLASSVGALQYRLSPGTLMNNA
                     WDGVREKGGTVADDALQAVKDRPMTVSGVVAAFFIFLARDPLWRFVSRLFRPRTPAEE
                     GTIKADLDHHDKNFDLTAPTVERSTSQGVNA"
     gene            complement(4560..4943)
                     /locus_tag="VF589_08775"
     CDS             complement(4560..4943)
                     /locus_tag="VF589_08775"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8257716.1"
                     /translation="MDARTQNPVDPSIGDLIHQLVDDGRSLVGAEVNLYKQVALYRAG
                     KAKTGIAALVAGGLLAFAGLIAFMVGLVMGLADLIGPVAGGLVVLAVTGIVAFILARW
                     GAAKMSALSGDPEEKAALKAGEQRA"
     gene            complement(4946..5713)
                     /locus_tag="VF589_08780"
     CDS             complement(4946..5713)
                     /locus_tag="VF589_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257717.1"
                     /translation="MPPRNPELPEGTDHIVNGAMETGAGTSASTGSSGDAFGGATAGG
                     GTTGAAGGGASSGFIGGGEAAGLGDGTGGTAFGGGTGGGGGGDFGGGSGGSGGGTPVK
                     QQLRQSAQTLKGQATDKVRSYADDGKQRATSALDDFAQVVNETAQTIDERLGAEYGEY
                     ARRAAGSISDFSARLRDKEVDELFDDARNVVRKSPALAIGTAAALGFALVRLIKSGMP
                     EEERDVQFTPDSTGTAGTGAGTQPQAPVVGTPSGTGV"
     gene            5867..7141
                     /gene="eno"
                     /locus_tag="VF589_08785"
     CDS             5867..7141
                     /gene="eno"
                     /locus_tag="VF589_08785"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX8257718.1"
                     /translation="MTAIIDVHARQILDSRGNPTVEVEILLEDGSMGRAAVPSGASTG
                     AHEAVEKRDGDDRRWLGKGVGQAVESVRGEIAEAVTGLDAEDQAEIDALMIDLDGTDN
                     KARLGANAILGVSLAVAKAAADARGLPLYRYVGGVAANLLPTPMMNILNGGEHADNAI
                     DFQEFMVMPVGAESLAEAVRCGAEIFHTLKKGLSEAGLSTAVGDEGGFAPALGRTADA
                     LDFIMKSVEQAGYRPGEDVLLALDCAATEYYHGGAYRMEGEGRTFSSGEMVDYLADLA
                     GRYPIASIEDGMAEDDWDGWKALTERLGGKVQLVGDDLFVTNPKRLAMGIERGIANSI
                     LIKVNQIGTLSETLEAVAAAHRAGYTSVMSHRSGETEDTTIADLAVASNCGQIKTGSL
                     ARSDRLAKYNQLIRIEEELGSAARYAGGSMFR"
     gene            7151..8188
                     /locus_tag="VF589_08790"
     CDS             7151..8188
                     /locus_tag="VF589_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257719.1"
                     /translation="MILPLLMLAAGTDLRPANMAQCRAEKAVAVTVEQLTAAAGQFEG
                     KCVTVSGPAWRRSMYSGVDGFYLTYRLGPDGWTGPEGRRRRVGLNFTGPWAGLESLRS
                     LSVTGVADSCERMRRVLDAEDEAEERLRKRTGSQAMTIRMLSGYCHFYPGPVVWVTDF
                     KQDVSRLYERRVGEEARRSVGSLVEVAPQWPRYSGLLALARQARKALGDRDRQRLTEL
                     FAKGGNPRPELEWLLEKPGTPFRELHDPDFDAPLAIFADAAGQYGVPAAAVPTLDGYV
                     CFCRTGDCQDKWPIAEIDTRMNPDHPYACFDVDTDGAGAVYLESRRGYAPLREPPGSA
                     FRPSGGKPGGR"
     gene            8610..9212
                     /locus_tag="VF589_08795"
     CDS             8610..9212
                     /locus_tag="VF589_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257720.1"
                     /translation="MEGFCHYATGPYLWLRGLRIGKPLAATRLVGEAMRAKVGDLAPA
                     PSSWPQLGFVRDHARRYLGALRRNARRTFLKLHVARERPIGSASEIERLAFGPHRGFA
                     ALPRLGVKPQTAIFVLRKDLADATSEPGSDDYDSHVCFCTQPDCSNRWPISAIDADNR
                     EDRPYVCARFGSYMIGPGRVVPAFTSELGRHGLPEPPSGR"
     gene            9276..9593
                     /locus_tag="VF589_08800"
     CDS             9276..9593
                     /locus_tag="VF589_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX8257721.1"
                     /translation="MAAGTRNTFELIKRAALPAVAVLIIANFLGYAIVGPNGVLSWGD
                     YRRQKAERAVELAQLEQEKTRLAHRAELLDPRKADPDLAEEMVRSELGLVRPDEVIIP
                     IQE"
     gene            9739..>10115
                     /locus_tag="VF589_08805"
     CDS             9739..>10115
                     /locus_tag="VF589_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8257722.1"
                     /translation="MAKAASSKRANRPDATAAPPPTSDLRPSRERPPEPARHQADRAE
                     LLEFYRQMLLIRRFEERAGQLYGLGLIGGFCHHYIGQEAVAVGLQSALEVGKDSVITG
                     YRDHGHMLAYGIDPKVIMAELTGR"
BASE COUNT         1521 a         3376 c         3558 g         1660 t
ORIGIN      
        1 aggagcggca gggccaggtc gaggcgcaga tcgccgacat cgacgaccgg ctgagccgcg
       61 cgatggtgat cgacccgacc accctcagcg gcgacaaggt cgtgttcggc gccaccgtcc
      121 atctgctcga cgaaaatgac aagccggtca aatatcagat cgtcggccag accgaggccg
      181 acgccaagat cggccgcatc tcctattcct cgccgctcgg ccgcgcgctc atcggccgca
      241 gcgtcggcga ggaggtcgag gtctcggcgc cgtcgggcga caaatattat tcgatcgaga
      301 aggtcgagtt catttagcct tatggcgatc gccgatcgcc gcgtcccggc acccacgacc
      361 gcgagcgccc actgatgcgt ccgcccgaca gctggaagcg ggcgcggctc acgctcggca
      421 tcgccggggt cgccgccttt gcctggctgg tcatttcaac cgcgggcctg ggcgatgtcg
      481 cgatgatgtg ggccggcttc atcccggcgc gggtcggcgg ggccgggggc gcggaggcga
      541 tggcgccgct ggcgctgacg ccgctcaccg ccaccctggt ccatatcggt ttcctgcacc
      601 tcgccttgaa cctcgtcatc ctgctggtct gcggccgccc ggtcgagaat atcctcggct
      661 ggcaggcaat cctgatcctg ttcctcgccg gcgcctatgc cgccgccgcc gcccattacg
      721 ggctcgatcc gtcggatatg tcgccgatgg tgggggtgag cggctcggtt tcggcggtgc
      781 tcggcgccta tgcgatgctg ttcagccgct tcaaggtgaa ggtggcgaac ccgaccctcg
      841 gcctctggct caacatcctc tggctgggcg ccgcctggat cctgttccag ctcgcggtcg
      901 gcttcacctt cgagcggccg ggcgcgccga ccggcgtcgc cgcccatatc ggcggcttcc
      961 tcgccgggct ggtcctcgcc aagcccctgc tcctgtttcg ctaccggcgc gcgtaaggga
     1021 ccagtggttg gtcggggctg gaccccggct ttcgccgggg aacgctgttc gagcgtgaca
     1081 cgggctaggc ccggtcggcc ggccagggac ggtcagccag agcgcggcgg ccagcatcag
     1141 cgcgcacccg gtaacgacgg tccagtagac gcgcggggtc tcggcgcggc cgtaatcgtc
     1201 gaggccgatc cgcaccttgc gcgtctgcag gaggcggacc agccgcaagg cgaacatcgg
     1261 aataaggagg aacccaacgg ccggcgggtc cccgccggtt cggtcgctga ggaacatcgc
     1321 gccgatgtag acgggcaaca ggatcgcgaa ggtgaacagc ccgaaccaat attccgccgg
     1381 ctcggcgccg cgctcgcgga catgctcacc aaagccccag cgcccggtct gcagcgcgcg
     1441 cgacgaacgc ggcgtcggcg agcagcagca gggcgatcaa gaccagccat ccgttcatgg
     1501 catagcggct tcctgccggc cgcccgacgc cgggcgcgct cgcggcggac cggcgccgcc
     1561 gcctccgtcg ctccgcgacg atatttcgaa gtccgaaacg gtgcaatacc ggagtatcac
     1621 tgtattgctg taatttagta cgcccactaa tctgttttcg atgggtaggg ggaagcatcg
     1681 ctcgatggcg gcgccttcgt cgccggcccg gcgccgatga gacgatcgtt gtgatcgcgt
     1741 tcgacggcgg gataatcttc ggggggtagt gccgctttgt gaccggacgc cgtcatcagc
     1801 ttgggcgggc aagccccgca tcgatccgat gcttcgcgcc ctgcgccctt tcttctcgcg
     1861 ttgcgggcca ggagcgcgac tttcggcagg attggaaagg agcggaatgg aacttgcgat
     1921 agcggttgtc atcttcatgg tcggccttgg actttggttc tgggcgattt ccctgctcac
     1981 gaagctgcat ggtttggcgc tcgccattac cggcgtcatc gtcaccatcg taacgctcgc
     2041 gatcacgatc gcgctggttc ggcagggcga ggagcatatc tatgcggcgc tggccttcat
     2101 gctgtcgggg atcgcggccc tggcgatcat cacgctgagc tgcgccaagg ccgggagcac
     2161 cgaagagcgg gagtgagcgc gttcattcct gccgcggcca catgagcccc atgccgccaa
     2221 gatggatcgg aaccggccgc aagcccatgc acaagatccc acgtgccgca tatcctcaac
     2281 tggccttccc gctaatcatc ctcgccacgc tatgggcgtt gccgattgat aggggatagt
     2341 cggcgcactt cggctagtgc tggaaggtgc cttcggaaag gtgcttgtcg gtgagccacc
     2401 gggcttcccg ccgcccgaca ttgtgtgagc ggcggcgggg ctggcgtcag ggttggggtt
     2461 ggccgtcgag gtcgggctcg agcaggcggt ggaggtggac gacgacatat ttcatctcgg
     2521 catcgtcgac cgtgcgctgg gcgttggagc gccacgcctc gagcgcgctg gcataatcgg
     2581 ggtagaggcc gaccaggtcg accgcctcga gatcgacgaa gtccagcccc tgcggatcgc
     2641 ggacgcggcc gccgaaaacg agatgcatct tgctctgctt gttgctcatg cgctgccctt
     2701 agccggttgg ttttgcgacg tcatccggga acaacccggc tcgggttcct gggttgcggg
     2761 gcggtagcgg gattcccggg ctggcgcttt agcggcagtc cggtcatcac agcggctggg
     2821 aaggcttggc accgcatacg gtcccggact tgcgcggtgt ctatgagagc ttggctggcg
     2881 gcccctgcct tcgcaggggc ccagaacccg ttcgcgggaa tgacggaggg gctcggcagg
     2941 cggcagggcc tgagcggatc agccgcggcg gcgcatgaat tcgacgaggg cgtcgaagcc
     3001 gttgcgctgg acgtaggaat cgaagtcggc ggtctgcgcc atggtgacgt tgacgccgct
     3061 gacgacgagg ttggtgacct tgtagccgcc gccgaccttg gtgaccgacc agatcgcgct
     3121 gaccggccgc gagccgggat tggcgatctg ggtcagcacc gcggcattgt tgccctggct
     3181 ctggacccgc accaccttga cgctggcgtc ggcataagcg tgcagccgat cggcataggt
     3241 accgacgatg aagccgggca aagcggcctt gtaggcagca tattgggcct ggctgatctt
     3301 cgggcgccag cgccggatca gccggtcgcc gatcgcctcg accgcgaaat gctgcgcgag
     3361 caggctgcgg aacttgccgc gggccgcgct cttctcgccg cgcagcgcgc cgaggccggt
     3421 gccggcgagg ctctggatga agcggccggg atcctggttc tggacctggg ccggggcggg
     3481 ggcggcgagg ctcgcggcgg cgatgccggc ggcggcccat ccgaatttga tcatgagtaa
     3541 ggctccatgg cagtgccgga acgcggcggc gggacgcgcg aaagcgcccc cgaccggggc
     3601 gattcctgaa cggaaccgcc taagcggggg cgccttgctt cgcgctgaac gattattgcg
     3661 gctgctgctg cccgctcttc accgtctgcg ccgccgcgcc ggcggaggtc ttgagcgcct
     3721 cgccggcgcc gccggcaatg tcgctcaatt tctgcttggc ggtgtcgcga ttgaggccga
     3781 gctcgtcgag cttgcccttg cccgcctcgg tggcggcgcg ggcggcctcg cgggcggtgt
     3841 cgttgatgcg ccgcccgaca tcgcccagca actccgcctc gcgccggctc ttggggagca
     3901 tggcggcgac cagggcgccg atggcgaggc cgccgatcaa ggctccgacc ggattggttt
     3961 cgatgccgtc ggcggtgcgg ctcttggcct gcgaggcgcc ctggcggacc gtgccatagg
     4021 ccgagccggt gcgctcgcgg gccgcgctat aggcggcgct ggccttgtcg cgcgccttgc
     4081 cggtaacggc cgagacgcgg ccgccgccgt cggcgccgtt gttggtggtg gtgccggtgt
     4141 cgttggcggt gtccgtaatg ctcatgcgtt cactccttgg ctggtcgacc gttcaaccgt
     4201 cggggcggtg aggtcgaaat tcttgtcatg atggtcgaga tcggccttga tcgtgccttc
     4261 ctcggcgggg gtgcgcggcc ggaacagccg cgagacgaac cgccagagcg gatcgcgcgc
     4321 gaggaagatg aagaaggcgg cgacgactcc cgaaacggtc atcggccggt ccttcaccgc
     4381 ctgcagcgca tcgtccgcga ccgtcccgcc cttctcgcgg acgccgtccc aggcattgtt
     4441 catcaaggtg ccggggctga gccggtattg cagcgcgccg acgctggagg cgagccgctt
     4501 cttggccgcc tcgacctccg cctgggcgcg ggtcagctcg gcttcgggcg tgggtccctt
     4561 catgctctct gctccccggc cttgagggcc gctttttcct cggggtcgcc ggagagcgcc
     4621 gacatcttgg cggcgcccca gcgggcgagg atgaaggcga cgatgccggt caccgccagc
     4681 acgacgaggc cgccggcgac cgggccgatc aggtcggcaa ggcccatcac cagcccgacc
     4741 atgaaggcga tcagcccggc gaaagcgagc aggccgccgg cgaccagggc ggcgatgccg
     4801 gtcttggcct tgccggcgcg gtagagggcg acctgcttgt agaggttgac ctccgccccg
     4861 accagcgagc gaccgtcatc caccagctgg tggatgaggt ccccgatcga cggatcgacc
     4921 ggattctgcg tccgggcgtc caaggtcaga cgcccgtgcc cgagggggtg ccgacgaccg
     4981 gcgcctgcgg ctgggtgccg gcgccggtgc ccgcggtgcc ggtgctatcc ggcgtgaact
     5041 gcacgtcgcg ctcctcctcc ggcatgccgg acttgatgag gcgcaccagc gcgaagccga
     5101 gcgcggcggc ggtgccgatc gcgagcgccg ggctcttgcg cacgacgttg cgggcgtcgt
     5161 cgaacaattc gtcgacttcc ttgtcgcgca ggcgcgccga gaaatcggag atggagccgg
     5221 ccgcgcggcg ggcatattcg ccatattcgg cgccgagccg ctcgtcgatc gtctgggcgg
     5281 tctcgttcac gacctgggcg aaatcgtcga gcgcgctggt cgcccgctgc ttgccgtcgt
     5341 ccgcgtagga gcggaccttg tcggtggcct gccccttcag ggtctgtgcg ctctggcgaa
     5401 gctgctgctt gaccggagtg ccgccgccgg agccgccgga gccgccgccg aagtctccgc
     5461 cgccgccgcc gccggtgccg ccgccaaagg cggtgccgcc ggtgccgtcg ccgaggccgg
     5521 ccgcttcgcc gccgccgatg aagccggagc ttgcgccgcc gcccgcagcg cccgtcgtgc
     5581 cgccgccggc cgtcgcaccg ccaaaggcgt caccgctcga tccggtgctc gcgctggtgc
     5641 cggcgccggt ctccatggcg ccgttgacga tgtggtcggt gccttcgggc aattcgggat
     5701 tgcgaggggg catagctgtt cctccatact ctgtgggtgt acgcattcga gccagaaaca
     5761 cacggctcgc ggcttacgtt ccgaggcgga aaggaaagtt gcgtcgccaa agccgcgccg
     5821 ataagcggcg caggctccct ataaactcga agcgaggccg taacaaatga ccgcgatcat
     5881 cgatgtccac gcccgccaga ttctcgacag ccgcggcaac cccactgtgg aggttgagat
     5941 attgctggag gacggcagca tgggccgggc cgcggtgccc tcgggtgcgt cgaccggcgc
     6001 tcatgaagcg gtcgaaaagc gcgacggcga cgaccgccgc tggctcggca agggcgtcgg
     6061 ccaggcggtc gagtcggtgc gcggcgagat cgccgaggcg gtgaccgggc tcgacgccga
     6121 ggaccaggcc gagatcgacg ctctgatgat cgacctcgac ggcaccgaca acaaggcgcg
     6181 gctcggcgcc aatgcgatcc tcggcgtcag cctcgccgtc gccaaggcgg ccgccgacgc
     6241 gcgcgggctg ccgctctacc gctatgtcgg cggcgtcgcc gcgaacctgc tgccgacgcc
     6301 gatgatgaac atattgaacg gcggcgaaca tgccgacaac gccatcgact tccaggaatt
     6361 catggtgatg ccggtcggcg ccgaaagcct ggccgaggcg gtgcgctgcg gcgccgagat
     6421 cttccatacg ctgaagaagg ggctgagcga ggcgggcctg tcgaccgcgg tcggcgacga
     6481 gggcgggttc gcacccgcgc tcgggcggac cgccgacgcg ctcgacttca tcatgaagtc
     6541 ggtcgagcag gccggttacc ggcccggcga ggacgtgctg ctcgcgctcg attgcgccgc
     6601 caccgaatat tatcatggcg gcgcctaccg gatggaaggg gaggggcgga ccttctcctc
     6661 gggcgagatg gtcgattatc tcgccgacct cgccggccgc tacccgatcg cctcgatcga
     6721 ggacggcatg gccgaggacg attgggacgg ctggaaggcg ctgaccgagc ggctcggcgg
     6781 caaggtccag ctggtcggcg acgatctgtt cgtgaccaac cccaagcggc tggcgatggg
     6841 catcgagcgc ggcatcgcca attcgatcct gatcaaggtc aaccagatcg gcacgctgtc
     6901 ggaaacgctg gaggcggtcg cggccgcgca ccgcgccggc tacacctcgg tcatgtcgca
     6961 ccgttcgggc gaaaccgagg acacgaccat cgccgacctc gccgtcgcca gcaattgcgg
     7021 gcagatcaag accggcagcc tcgcccgctc ggaccggctc gccaaatata atcagctcat
     7081 ccgcatcgaa gaggaactgg gctcggccgc ccgctatgcg ggagggagca tgttccgcta
     7141 agctgcgggc atgattctgc cgttgctgat gctggcggcg gggaccgatc tccggcccgc
     7201 gaacatggcc caatgccgtg cggagaaggc tgtcgcggta acggtcgagc agcttaccgc
     7261 cgcggccggg cagttcgagg gcaagtgcgt gacggtgagc gggccggcct ggcgtcggtc
     7321 catgtacagc ggcgtcgacg gcttctacct gacctatcgg ctggggccgg acggctggac
     7381 cgggccggag gggcgtcgtc gtcgcgtcgg ccttaacttc accggcccgt gggcggggct
     7441 cgaaagcctg cggtcgctga gcgtcacggg cgtcgccgac agctgcgagc gcatgcgacg
     7501 cgtgctggat gcggaggacg aggccgagga gcggctgcgc aagcgaaccg gcagccaggc
     7561 catgacgatc cggatgctca gcggatattg tcatttctat ccggggccgg tcgtctgggt
     7621 gaccgatttc aagcaggacg tatcgagatt gtacgagcgg cgcgtcgggg aggaggcccg
     7681 gcggtcggtc ggcagtctcg tcgaggtcgc gccgcaatgg cctcgctatt cgggcctgct
     7741 cgctttggcc cggcaggctc gcaaggcgct gggcgatcgc gatcggcagc gccttaccga
     7801 attgttcgcg aagggcggca acccgaggcc ggagctcgaa tggctgctcg agaaaccggg
     7861 aacgccgttc cgcgagctgc acgaccccga tttcgacgcg ccgctcgcaa tcttcgccga
     7921 cgcagccgga caatatggag ttcctgccgc cgcggtgccg acgctcgacg gttacgtctg
     7981 cttttgccga accggcgact gccaggacaa gtggccgatc gccgagatcg acacgcggat
     8041 gaatcccgat catccctatg cctgcttcga cgtggatacc gacggcgcgg gcgccgttta
     8101 tctggaatcg cgccgaggtt atgccccgct ccgggagccg cccgggtcgg cgttccgccc
     8161 gagcggcggg aagccgggcg gccggtgatc gccgggctgg ccggtttgct cgccgccctt
     8221 gccgccccgg caggcgccgc ctcgccggcg ctcgctgccg aagccaaagc tcgcgaacgg
     8281 tgcgatcccg cgcgggcgga gccggcgacg atcgcggcga tcatggccga ccggccgggc
     8341 tatgaggggc gctgcgtcgc cgtcacggcg atcagcaacg gctatctgct gttcacgtcc
     8401 gtcgagggct attaccgggc gggaccgctg gtgggcggcg atcccgatgc caatccggcc
     8461 gaccgggagc ggcttgggct cgacaattgg tcgatgatgc agccggtgcg cggcggcggc
     8521 ctccatcatg tcaccgcgat cggccgggtc gaggattgcg ggcggatcac cggcatgttc
     8581 aaggcggcgg cgggcgagaa tgatttcgtg tggagggctt ctgccattat gcaaccggtc
     8641 cctatctctg gctgcgcggc ctccggatcg gaaagccgct cgcggccacc cggctggtcg
     8701 gcgaggcgat gcgggccaag gtcggcgacc tcgcgcccgc gccttcgtcc tggccgcaac
     8761 tcggcttcgt gcgggaccat gcgcggcgct atttgggcgc gctgcggcga aatgcccggc
     8821 gcactttcct caagctccac gtcgcgcggg agcgcccgat cggcagcgcg tcggagatcg
     8881 agcgtctcgc ctttgggccc caccgcgggt ttgccgcgct tccgcgcctt ggggtgaagc
     8941 cgcagacggc gatcttcgtc ctcaggaagg atctcgccga cgcgacgagt gagccgggca
     9001 gcgacgacta cgactcccat gtttgcttct gcacccagcc ggactgctcg aaccggtggc
     9061 cgatcagcgc catcgatgcc gacaaccgtg aggatcggcc ctatgtctgc gccagatttg
     9121 gatcctacat gatcggtccg ggccgggtgg tgccggcctt cacgtccgag ctcggccgcc
     9181 acggcctgcc cgagccgccg tcgggccggt gacatatttg cacgcagcgt cgaaaagggc
     9241 ttgattcggc ggcgctttcc tgattctgtt aaccaatggc ggcgggcacc aggaacacct
     9301 ttgaactgat caagcgggcg gcgcttcccg cggtcgcggt gctgattatc gccaatttcc
     9361 tcggctatgc gatcgtcggc ccgaacggcg tcctctcctg gggcgattac cgccggcaga
     9421 aagcggagcg ggcggtcgag ctcgcccagc tcgagcagga aaagacccgg ctcgcccacc
     9481 gcgccgaatt gctggatccg cgcaaggccg atcccgacct cgccgaagaa atggtgcgca
     9541 gcgagctcgg cctcgtccgc cccgacgaag tcatcatccc gatccaggaa taagcggccc
     9601 cgccgctggc gagccttccc gatagccggc ccccgctagc agcttcgacg cggccatgac
     9661 catggccgga gccatgttgc gcgccccggc gtttgtcgcc atagagccgc caaatcactg
     9721 caccggaaag gaagggcctt ggccaaggca gcatcgtcga aacgcgccaa tcgaccggac
     9781 gcgacggccg cgccgccccc aacctccgac cttcgcccca gtcgcgagcg cccgcccgag
     9841 ccggcgcggc accaggccga ccgcgccgaa ttgctcgaat tctaccgcca gatgctgctc
     9901 atccgccgct tcgaggagcg cgccggccag ctttacgggc tcggcctgat cggcggcttc
     9961 tgtcaccact atatcggcca ggaggcggtc gcagtcgggc ttcagtcggc gctcgaggtc
    10021 ggcaaggaca gcgtcatcac cggctaccgc gaccatggcc acatgctcgc ctacggaatc
    10081 gatcccaagg ttattatggc ggagctcacc gggcg
//
LOCUS       DASVRK010000223        19881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_543285, whole genome shotgun sequence.
ACCESSION   DASVRK010000223 DASVRK010000000
VERSION     DASVRK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 19881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19881
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543285"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1841)
                     /locus_tag="VF589_08810"
     CDS             complement(90..1841)
                     /locus_tag="VF589_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX8257723.1"
                     /translation="MSRKREAAPLAPTPTADRLPRRLADFELVCDALDYAAKGGRGLN
                     FHDARGVLARAYSYAELREDALAAAYRLIAEGIKPGDRVALIAETGAEFASLFFGVIY
                     AGAWPVPLPLPTSFGGRDSYIDQLAIQLKSSDPSLLFYPAELTGMAGAAASAVGVKGV
                     DWETFGRWSGPAAALPRPHPDDIAYLQYSSGSTRFPHGVAVTHRALLNNLAAHAHGVE
                     VIETDRCVSWLPWYHDMGLVGCLLSPVANQVSADYLKTEDFARRPLAWLDLISRNAGT
                     TLSYSPTFGYDICARRIGSQSKVAERFDLSRWRVAGNGADMIRPDVMQAFVDAFADAG
                     FNARSFTPSYGLAEATLAVSIMPVGEGIVVELVEETLLAGGGPAERDRPQRYRAIVNC
                     GKPVRDMEVAIRADDGGALPERAIGKVYARGPSIMNGYFRDDVATEACLDGDGWLDTG
                     DMGYMSGGYLYIVGRAKDMIIINGRNHWPQDIEWAVEQLPGFKAGDIAAFSITTPGGE
                     ETPAVLVQCRTSDVVERARLRTEIRERVRSITGVNCVIELVPPRTLPRTSSGKLSRAK
                     ARNLYLSGEIQPYDIAA"
     gene            1964..2518
                     /locus_tag="VF589_08815"
     CDS             1964..2518
                     /locus_tag="VF589_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX8257724.1"
                     /translation="MAQSWRVKPQRHNRPRPALDGEALERLALFYVGRYATTRARLAS
                     YLTRKLRERGWEGDAPAPVAAVVERLSSLGYVDDGAFATARAASLQRRGFGERRVAQA
                     LQAAGIAEADAAEARQDAREGALTSALRFAERKRLGPYAAAPPDRRDREKGFGAMLRA
                     GHSPAIARQVLDLGPGQFPDCDTE"
     gene            2615..3202
                     /locus_tag="VF589_08820"
     CDS             2615..3202
                     /locus_tag="VF589_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX8257725.1"
                     /translation="MLADENASHPDGGDGARPGLEGEAAEALIKLTGTMKWFDATRGF
                     GFLVSDQAKGDVLVHFSVLKEHDRRSLPEGAVVECLVAEQERGLQARKILSIDLSHAV
                     VPELSRPRTGGDRIDPSALLDEAGAFEPVRVKWFNRLKGYGFLVREEDDTQDIFVHME
                     TIRRGGLADLTPDEPLRARVAMGRKGPLAVEVEPR"
     gene            3199..3699
                     /locus_tag="VF589_08825"
     CDS             3199..3699
                     /locus_tag="VF589_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX8257726.1"
                     /translation="MKAGLPLVAVASAMVLLGCRGEPGTAATQAAGSQPGRPTLPVVA
                     LQIRSGERTHDFSVEVARTEEEQARGLMFRESLAPGAGMLFPFDGPRPASFWMKNTLI
                     PLDMIFVRADGLIARIAARTVPHSLESVGVGEPVVAVLEIAGGRAAELGIREGDRVAW
                     PGGPTL"
     gene            3760..4140
                     /locus_tag="VF589_08830"
     CDS             3760..4140
                     /locus_tag="VF589_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEX8257727.1"
                     /translation="MGLLKQIFTWWEGATIGTALFSWRHGEKVGSDGLGNAYFRSKKG
                     DRRWVIYNGSNDASRIPPDWYAWMHGMIDGLPDEALPPARKFQKPPKGNLTGTPDAYR
                     PSGALERGGQRQAASGDYQAWTPE"
     gene            4144..4674
                     /locus_tag="VF589_08835"
     CDS             4144..4674
                     /locus_tag="VF589_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX8257728.1"
                     /translation="MRGLLRDNLLEAAVGLLVVLLAGWFILFAWNRTGGGARDSIQVK
                     AMFPAANGVSVGTDVRVAGLKVGSVAAQRLDPASYQAEVTLALNRSVNIPSDSSAAIT
                     AEGLLGGTYIALQPGGAPTRLKDGDTILDTQGSVDMMSLVGSLINRSGGEQAEPAAPD
                     AAPAQGLGTMEEPAAK"
     gene            4842..5075
                     /locus_tag="VF589_08840"
                     /pseudo
     CDS             4842..5075
                     /locus_tag="VF589_08840"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
     gene            complement(5111..5863)
                     /gene="aat"
                     /locus_tag="VF589_08845"
     CDS             complement(5111..5863)
                     /gene="aat"
                     /locus_tag="VF589_08845"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX8257729.1"
                     /translation="MAPLDPDLLLRAYAIGVFPMADSRNAQEVFWVEPRRRAIIPLHK
                     FQMSRSLRKTIRSGRFTVTRDKAFAAVVAHCAAREETWINAEIERSFNRMHRLGHAHS
                     IECWQDEQLVGGLYGIKLGAAFFGESMFSLRTDASKVALAWLVARLRVGGFSLLDCQF
                     MTDHLRSLGAVEISQKDYVSMLSAALAGEGLGAGGGAAFGAGDGDAFGAELERRGAEF
                     GALERLLAADSSRTVDAAASGWVIAQLLAQTS"
     gene            complement(5929..6276)
                     /gene="arsC"
                     /locus_tag="VF589_08850"
     CDS             complement(5929..6276)
                     /gene="arsC"
                     /locus_tag="VF589_08850"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832239.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX8257730.1"
                     /translation="MKATIYHNPRCGTSRKTLELLEQEGAEVTVVDYLKHPPSKDELR
                     RLYDRAGISPREGLRAKEQLAKDLDLVEGAVSDDALLEAMVANPVLINRPLVETEKGV
                     RLCRPQELVREIL"
     gene            complement(6289..7164)
                     /locus_tag="VF589_08855"
     CDS             complement(6289..7164)
                     /locus_tag="VF589_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257731.1"
                     /translation="MSAGRKLLAAALALLAGGCGLGEDGMVLLPDPAWRASIVARSGD
                     GFTVPDGLWWANGRLYMADEGGSAIRVWTGKGRSVALANAADGLRSPEDLAVDGQGNV
                     FFTDDEAGGVRGVDPSGRTVLLARRSAALGATEGIALAPSGAILVGDADGHRVLGISR
                     TGQVSVVVKGITKPESMAFDEAGNLYIADNEDDVLYLLRPDGSLHRPLANRDGFSPET
                     IWYARGALYISDSANGKLHVYSPEDGLSTIAVFGGDLAQVAGVTVDADGAIYLAVQTD
                     LKQKRGYLLRLDRGG"
     gene            complement(7161..7961)
                     /locus_tag="VF589_08860"
     CDS             complement(7161..7961)
                     /locus_tag="VF589_08860"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8257732.1"
                     /translation="MLTGLDRGLYQLLNGLAGRSPLFDTLVALPLESNLVKAGVIGAC
                     FIFAWHGAGDDAAIKRRRRILIVTALASVMAIATTKLISTSVFLPRPYVLSQKTYLLD
                     DGALTQAPLLPYRVPLNERNGAEHDELKRGEIEQNDLGSFPSDHAGFYATLAAGIALA
                     SRGAGLVAIGWLLAVTLASRVITGQHSPLDLAGGAAIGLAILAALQWLASRWGSRLTE
                     PITAWTIRHPALSASLAFLFVFEATNALDDVRMILDAAKTIAALGGAA"
     gene            complement(8124..9464)
                     /locus_tag="VF589_08865"
     CDS             complement(8124..9464)
                     /locus_tag="VF589_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX8257733.1"
                     /translation="MAAAPGEAGSGQTARAYAAAVLYTLAALAFRLALDPYLAGVQYI
                     TFFAAVIAIAYLYGIGPGLVAAILGALASWYFLVPERYSFRIDDPAQGNTLILFFVVA
                     CATVVLLGRARGVTRELLATERALGRSEQRYRDMFEQVNDIVFTADLDQNITDCNQAA
                     ARAVGAPREALIGRNIAEFVSPEGYEETSRRLREKLERGGNTQYDIEVRSRTGRQLHW
                     EINSGLAADADGRVIGLFAIGRDVTERRAAEQRQQLLVNELNHRVKNTLAIVQALAQQ
                     SFKGAGLPPAMREAFEGRLSALSAAHNLLTDQHWQAASIRQIVRDGASFPGAGEDRVT
                     MEGPDLLLPPQTAVGLALAVHELTTNAVKYGALSVAEGRVAITWRTEGERLNLVWEEQ
                     GGPPVEPPRRSGFGTRLIERALAAELGGRAVLSFRPEGVRCEVDAPLPHLPEAG"
     gene            complement(9465..10289)
                     /locus_tag="VF589_08870"
     CDS             complement(9465..10289)
                     /locus_tag="VF589_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8257734.1"
                     /translation="MSSDQNYDTAAIMGGLYGDGIIGLKGAFPPAWADAMREDIERLY
                     EEAKAVPGGALPRGPQRFYVEVQPERVGGFVDIVTHPWFVAVSEAVLGPDYKIVEIGF
                     DIPFPGAADQPWHRDFFVPEATTKGRRLNSLAFNLTAVDTVPEMGPFEIALGTQWDQF
                     EGCDKGMFPPRELWPRYEGRAVQKMPQRGDMAARSALTIHRGTANRSNAARPVLVVGV
                     DAPDATNSQHHDLQVTRAYAATLPPRVLDHLIYRTVDALETVVQHHVIEGLLEPAY"
     gene            10616..10759
                     /locus_tag="VF589_08875"
     CDS             10616..10759
                     /locus_tag="VF589_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257735.1"
                     /translation="MEVEREPVTTEKETVIIERGGGGGVIAAIAFLILVLAVLAYFGM
                     LPI"
     gene            complement(10773..10961)
                     /locus_tag="VF589_08880"
     CDS             complement(10773..10961)
                     /locus_tag="VF589_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257736.1"
                     /translation="MTLPPTGSDETRATPPAEQQGAEGGGPKRRKQRVRAGLAFGIGS
                     AAIVAALLYANRPKRKDG"
     gene            complement(11052..11894)
                     /locus_tag="VF589_08885"
     CDS             complement(11052..11894)
                     /locus_tag="VF589_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257737.1"
                     /translation="MVQPEADLPAPVFSGSSFPAPALPAPVANPGSGAAASESLGWMP
                     WAVGLAALLALLALVMLRRRRDAFAGGGYLRERAARVPAPAPRPVPPPAPVAVPRAPQ
                     PRIELDFQPDRVVLTPDKAEIHYRLTVRNAGDAAAADIRVAIDMFNAGAGQEAELAAF
                     FAEQQLLGSDGGEWRLAPGQEEKLQGVASMANADIRGIELQGRRLFIPLVGFNFLCGD
                     GGGGRVQTATSYLVGREADPPGEKMGPFRLDTGPRVYRKVGQRPNLPPPPKEKKRVRV
                     RLEA"
     gene            12429..12629
                     /locus_tag="VF589_08890"
     CDS             12429..12629
                     /locus_tag="VF589_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257738.1"
                     /translation="MSKSSDSNKSNTVNSGGAPKPTQEERDREFADITGNVRLKGEDP
                     SPDDRGETTSREPAPAIDIDNS"
     gene            complement(12657..13586)
                     /gene="dmeF"
                     /locus_tag="VF589_08895"
     CDS             complement(12657..13586)
                     /gene="dmeF"
                     /locus_tag="VF589_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEX8257739.1"
                     /translation="MTHSHSDDRWQHDHAFLGEHHARNELRTRLVIALTLAMMVAEVT
                     AGVMFGSMALLADGLHMATHAGALALAAAAYAYARRHLHNPRFAFGTGKVGDLAGFTS
                     AVTLAIAALLIGWESAERLVEPVMIDFDEAIAVACLGLAVNLVSAWLLHGGGGQRHDH
                     GDHQHHHHHHHDHNLRAAYLHVLADALTSVLAIAGLVAGSRFGLVWMDPAIGILGALV
                     IARWSWGLMRDSGAVLLDAAGDPRLAEAIRAKLETGGDLVPDLHVWRVGPGHMAAVLT
                     VVSHAPESPSAYKARIAHLKSLCHITIEVHRCE"
     gene            14292..14669
                     /locus_tag="VF589_08900"
     CDS             14292..14669
                     /locus_tag="VF589_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257740.1"
                     /translation="MKKTITAIIIAGTVALGGCARNYEDDGALQGVGTGAAVGALAGA
                     GVGAVVGGISPIEGALVGAAVGGIAGAIWADQDGDGQADGYYQEGTYYPGRPPAAASA
                     PPYQAPYQAPPPEPVRRSGERGR"
     gene            14682..16271
                     /locus_tag="VF589_08905"
     CDS             14682..16271
                     /locus_tag="VF589_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8257741.1"
                     /translation="MNQSNVGAGRQRWPAQISIRGLCLAAALAAAALPAYVPAFAQGS
                     PGAVAAALKSSAKSDRELRAFYQARGHRPLWFAAGPASPDAERVVDLIRSASLDGLDP
                     GRYKPKALAEAVAKARGGSPKAVGKADAMLSRAFAAYVRDVRRPRNVGMVYVDRELAP
                     VQPGVRAVLDAAAAAPSLAAHLDGIAWMNPLYGRLRSGLLAYRERWARLPQAAIAPGP
                     VLRADGDRVRMLRHRLGLPADGSFDEGLTSALRSFQAAHGLPDDAVAGPRTLAALNAD
                     PRGQERLIRLNMERARGLPANPGRRYILVDAASARLWAYEDGRVVDTMRVIVGKPTEQ
                     TPMMAGLIRYAMVNPYWNIPPDLVRNRVAPAVLKSGISYVKSKRYEVRSDWAEDSPVV
                     NPKDVDWAAVAAGRQELPVRQLPGRDNAMGKMKFMLPNQLGIYLHDTPEKALFKEEER
                     RFSSGCVRVEDAPRLARWLFGKPLKVPARGSERRVDLPRPVPVYITYLTAAPAEQGIA
                     FSADAYNRDARALGARSMAAR"
     gene            complement(16529..16858)
                     /locus_tag="VF589_08910"
     CDS             complement(16529..16858)
                     /locus_tag="VF589_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEX8257742.1"
                     /translation="MPQILNYHAHIYFDPPELAAAQALAGEARERFGVSVGRFHERAV
                     GPHPRGSCQLTVAPERFGEFAQWAAVSRGELTLFVHATTGDDLADHTRHVIWFGASEP
                     LDLSIFG"
     gene            16946..17500
                     /locus_tag="VF589_08915"
     CDS             16946..17500
                     /locus_tag="VF589_08915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX8257743.1"
                     /translation="MDGPPAPTSTAALRAGPRRGIAAALLTRPRARDFGRMALEAVWL
                     VPLLLAIGWLGDLMHWRPTFDATIARLALVAIVAPALGEELLFRAALLPNPAPARWPL
                     GAIAASVALFVLWHPIQALVFGPHWAETVLDPWFLAAVAATGIALARLYLATGSIWPC
                     VILHWLVVVGWKALLAGPSPWLPS"
     gene            complement(17505..17975)
                     /locus_tag="VF589_08920"
     CDS             complement(17505..17975)
                     /locus_tag="VF589_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257744.1"
                     /translation="MLAVSAPAGAAEWALQPGDGGRGARLTFGLGEPVSYRFECAAGD
                     VIVTQTGVTKLLDVKTRSQVDDGAQVLPPGAAMMGLFGGTGEPGFMPAAAVKNPAGGW
                     DLTIRLPGNDKRLKAVGRSEMISLFTTGYTMAVSMDAAARAIWQRFMERCATGR"
     gene            18129..18758
                     /locus_tag="VF589_08925"
     CDS             18129..18758
                     /locus_tag="VF589_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8257745.1"
                     /translation="MACPAEAIIGTYQRHARAWDRARSRALDERRWLDKFADLLPQGA
                     QILDIGCGGGEPLARHLIERGYRLAGIDASPPLIETCRDRFPDHDWHVADMRELALGR
                     QFDGLLAWDSFFHLAPADQRRMFPIFRGHAAPGAALLFTSGPAHGEAIGSFEGEPLYH
                     GSLDPDEYRDLLARSGFTVVAHQVEDPECGHHTLWLARMQAVAISAPAH"
     gene            18769..19167
                     /locus_tag="VF589_08930"
     CDS             18769..19167
                     /locus_tag="VF589_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8257746.1"
                     /translation="MIVRTATCRCGQLRATCTGEPVRISVCHCLACQKRSGSAFAVQA
                     RWPDDRVELAGEPSSWSQTGDSGSLATFLFCANCGATLAYASDAMPGLTAIAVGAFAD
                     PNFPPPEYSVWEVRKHGWVAITGEGIEHFE"
     gene            19402..>19881
                     /locus_tag="VF589_08935"
     CDS             19402..>19881
                     /locus_tag="VF589_08935"
                     /inference="COORDINATES: protein motif:HMM:NF033947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cistern family PEP-CTERM protein"
                     /protein_id="HEX8257747.1"
                     /translation="MTLTKSFAIAAAMLLSTAANAGTINVTPSDIGNSFTVGFDGFAN
                     DSVIDGLSAEATFTLTEATGSSYTFDYDIANTSGGAIDASRVSIFGFNTDPTISGATS
                     TGLFSEVNTNANVPSGFGQVDVCFKGSGDGNSCSGGGGTGVWMGDSTSGSFTLTFDQA
                     "
BASE COUNT         3011 a         7162 c         6639 g         3069 t
ORIGIN      
        1 atggcttttc cgcggccgac cttagccgcg caagcgttag agaagcgtta gggctatcgc
       61 ggcgccgcgt cggtccgggc cggcggcggt caggccgcga tgtcgtaggg ctggatttcg
      121 ccggagagat agaggttgcg cgccttggcc cgcgagagct tgccggagct ggtgcggggc
      181 agggtgcgcg gcggcaccag ctcgatcacg caattgaccc cggtgatcga ccgcacccgc
      241 tcgcggatct cggtgcgcag ccgcgcgcgc tcgaccacgt cggaggtacg gcactggacc
      301 agcaccgccg gcgtctcttc gcctccgggc gtggtgatcg agaaggcggc gatgtcgccg
      361 gccttgaagc ccggcaattg ctccaccgcc cattcaatgt cctgcggcca atggttgcgg
      421 ccgttgatga tgatcatgtc cttggcgcgg ccgacaatgt agaggtagcc gcccgacatg
      481 tagcccatgt cgccggtgtc gagccagccg tcgccgtcga ggcaagcctc ggtcgcgacg
      541 tcgtcccgga aatagccgtt catgatcgaa ggcccgcggg catagacctt gccgatcgcg
      601 cgctcgggga gggcgccgcc gtcgtcggcg cggatcgcca cctccatgtc gcggaccggc
      661 ttgccgcaat tgacgatcgc ccggtagcgc tgcgggcggt cgcgctcggc ggggccgccg
      721 ccggcgagca aggtctcctc caccagttcg acgacgatcc cttcgccgac cggcatgatc
      781 gacaccgcca gcgtcgcctc ggcaagccca tagcttggcg tgaagctcct ggcgttgaag
      841 ccggcgtcgg cgaaggcgtc gacgaacgcc tgcatcacgt ccggccggat catgtcggcg
      901 ccgttgcccg ccacccgcca gcgcgacagg tcgaaccgct cggcgacctt gctctggctg
      961 ccgatccggc gtgcgcagat gtcgtagccg aaggtcggcg aatagctcaa agtcgtaccg
     1021 gcgttgcggc tgatgaggtc gagccaggcg agcggacggc gcgcgaaatc ctcggtcttg
     1081 agataatcgg ccgaaacctg gttcgcgacc ggcgacagca ggcagccgac caggcccatg
     1141 tcgtgatacc atggcagcca ggagacgcag cggtcggtct cgatcacttc gacgccatgg
     1201 gcgtgcgcgg cgaggttgtt caggagggcg cgatgggtga ccgcgactcc gtgcggaaag
     1261 cgggtcgatc cgctcgaata ttgcagatag gcgatgtcgt cgggatgggg gcggggcagg
     1321 gcggcggccg gccccgacca tcggccgaag gtctcccaat cgactccctt gacgccgacc
     1381 gccgacgcgg cggctccagc catcccggtg agttctgccg gatagaaaag caggctcggg
     1441 tccgagcttt tcaactggat cgcaagctgg tcgatatagc tgtcgcggcc cccgaacgag
     1501 gtcggcagcg gcagcggcac cggccaggcg ccggcataga tgacgccgaa gaacaaggat
     1561 gcgaactccg ccccggtctc cgcgatcaat gctacccggt cgcccggctt gatgccctcg
     1621 gcgatcagcc ggtaggccgc ggccagcgca tcctcgcgca gctcggcata gctataggcg
     1681 cgggcgagaa ccccgcgggc gtcgtggaaa ttgaggccgc ggccgccctt ggccgcgtaa
     1741 tcgagggcat cgcagacgag ctcgaaatcg gccagccggc gcggcaggcg gtcggccgtc
     1801 ggggtcggcg caagcggcgc cgcttcgcgc ttgcgcgaca acattcggtc ctggggctga
     1861 tgcagcacta tgcgcgaatt cctcggcttt ccgggcctcg ttccgggggt tgcaacctgg
     1921 ctcctccttt cggcacgagg cacgttcata ttccggcccg actatggcac aatcatggcg
     1981 cgtgaaaccc cagagacata atcggccccg tccggcgctc gacggagagg cgctcgagcg
     2041 gctcgcgctc ttctatgtcg gccgctacgc caccacccgc gccaggctcg catcctatct
     2101 cacccgcaag ctcagggagc gcggctggga gggcgacgct ccggcgccgg tagcggccgt
     2161 cgtcgagcgc ctctccagcc tcggctatgt cgacgacggc gccttcgcga ccgcccgggc
     2221 ggcgtcgctc cagcggcgcg ggttcggcga gcgccgcgtc gcccaggcgc tgcaggcggc
     2281 cggcatcgcc gaagccgacg cggccgaggc ccgccaggac gcccgcgagg gagcgctgac
     2341 ctcggcgctg cgctttgccg agcgcaagcg gctcggcccc tatgccgccg ctccgcccga
     2401 ccggcgcgat cgcgagaagg ggttcggcgc gatgctgcgc gccggacact cgccggccat
     2461 agctcgccag gtgctcgatc tcggccccgg ccaattcccg gattgcgaca ctgaatgaaa
     2521 ttccctgtcg gaaaagttca cattccagca aaagccatga tactcatcgc gaagttcgtg
     2581 ttagattagg cgttaaatga cctcacgccg gccaatgctt gcagacgaaa acgcctcgca
     2641 tccggacggc ggcgacggcg cgcgccccgg tttggagggc gaggccgccg aggccttgat
     2701 caagctcacc gggaccatga aatggttcga cgcgacccgc ggtttcggct tcctggtcag
     2761 cgatcaagcc aaaggcgacg tcctcgtcca cttcagcgtc ctcaaggagc atgaccggcg
     2821 gagccttccc gaaggggcgg tggtcgaatg cctggtcgcc gagcaggaaa gggggctgca
     2881 ggcccgcaag atcctctcga tcgacctcag ccatgccgtc gtcccggagc tcagccgtcc
     2941 gcggaccggc ggcgatcgga tcgatccgtc cgctttgctc gacgaggccg gggcgttcga
     3001 gccggtccgg gtcaaatggt tcaaccgcct caaaggctat ggcttcctcg tgcgcgagga
     3061 ggatgatacg caggacatat tcgtccacat ggaaaccatc cgccgcggcg ggcttgccga
     3121 tctcacgccg gatgagccgc tccgcgcccg cgtcgcgatg gggcgcaagg gcccgctcgc
     3181 ggtcgaggtc gagccgcgat gaaggctgga ctgccgctgg tcgcggtggc gtcggcgatg
     3241 gtgctgctcg gctgccgggg cgagccgggg acggcggcaa cccaggcggc gggctcgcag
     3301 cccggccgcc cgacgctccc ggtcgtggcg ctccagatcc gcagcggcga gcgcactcat
     3361 gatttcagcg tcgaggtcgc ccgcaccgag gaggagcagg cgaggggcct catgttccgc
     3421 gaaagcctcg cccccggcgc cggcatgctg ttcccgttcg atgggccccg gccggcgagc
     3481 ttctggatga agaacacgct gatcccgctc gacatgatct tcgtccgcgc cgacggcttg
     3541 atcgcccgga tcgccgcgcg caccgtgccc cactcgctcg aatcggtggg ggtgggcgag
     3601 ccggtcgtcg cggtcctcga aatcgccggc ggccgcgccg cggagctcgg catccgcgag
     3661 ggcgaccggg tcgcgtggcc cggcggcccc accctctaag ccgttgccga ggcggacaga
     3721 gcttgcgcac cgccgccgtt cggcgctagg cgggccgcga tgggcctgct caagcaaatc
     3781 ttcacctggt gggaaggtgc gacgatcggc accgctttgt tcagctggcg ccatggcgaa
     3841 aaggtcggca gcgacggcct cggcaatgcc tatttccgct cgaaaaaggg cgatcgccgc
     3901 tgggtgatct ataacgggtc caacgatgcc agccgtatcc cgcccgattg gtacgcctgg
     3961 atgcacggca tgatcgacgg actgccggac gaagcgctgc cgccggcccg caagttccag
     4021 aagccgccca agggcaatct caccgggacg cccgacgcct atcgcccctc cggagcgctg
     4081 gagcgcggcg ggcaacgcca ggcggcgagc ggcgactatc aggcctggac gcccgaataa
     4141 gccatgcggg gccttctccg ggacaatctt cttgaagcgg cggtcgggct tctggtcgtg
     4201 cttctggccg gctggttcat cctgttcgcc tggaaccgca ccggcggcgg cgcgcgcgat
     4261 tcgatccagg tgaaggcgat gttcccggcc gccaacggcg tgtcggtcgg caccgatgtc
     4321 cgcgtcgccg ggctcaaggt cggcagcgtc gccgcgcagc ggctcgatcc cgcaagctac
     4381 caggccgaag tgacgctggc gctcaaccgc tcggtcaaca tcccgtccga cagcagcgcc
     4441 gcgatcaccg ccgaagggct gctcggcggc acctatatcg cgctccagcc cggcggcgcg
     4501 ccgacccggc tcaaggacgg cgacaccatc ctcgacaccc agggttcggt cgacatgatg
     4561 agcctggtgg gctcgctcat caaccgctcc ggcggcgagc aggccgagcc ggccgcgccc
     4621 gacgccgcgc cggcccaagg cctcggcacc atggaagagc cggcggcaaa gtgacccggc
     4681 tccttctgat cgctccgctc gccggcctcg cgctcctcgc cgcctgcaat tccggcgacg
     4741 tgccggcgcc caagcagccg accaaggccg aggccgaacc ggagccggag acggtcgaca
     4801 tcccccagac ggtcaccgcc atcgcccccg gaacgacccc gatggctgag cggaccgccg
     4861 tcgtcgggct gctcaacaag cgcaacggcc tctcgcgcga cctgacgctg aagcccggcc
     4921 aggcgatgcg cgtgggcgac gtggtgctgc gcctgcgcgc ctgcgagacc accgcgccgc
     4981 acgagattca gcagctcacc ggcgccttcg tccagctcga cgtgcgcaat cctcagggac
     5041 agttccagcg cgtcttttcg ggatggctgt acaaggaggc gccgtcgctg aacgtcgtgg
     5101 agcacccgat ctacgacgtc tgggccaaga gctgcgcgat gacccatccg gacgccgccg
     5161 cgtccaccgt acgagaggaa tcggcggcga gcagccgctc gagtgcacca aattcagcgc
     5221 cgcgccgttc cagctccgct ccaaaggcgt cgccgtctcc agcgccgaag gccgcgccgc
     5281 cgcccgcgcc cagcccttcg cccgccaagg cagcggacaa catcgagaca taatccttct
     5341 ggctgatctc gaccgcgccg aggctgcgca gatggtcggt catgaactgg cagtcgagca
     5401 gcgagaatcc gcccacccgc agccgcgcca ccagccaggc gagcgccacc ttggaggcgt
     5461 cggtccgcag cgagaacatg ctctcgccga aaaaggcggc gccgagcttg atgccgtaaa
     5521 gccctccgac caattgctcg tcctgccagc attcgatcga atgggcgtgg cccagccggt
     5581 gcatgcggtt gaagctgcgc tcgatctcgg cgttgatcca ggtctcctcg cgggccgcgc
     5641 aatgggcgac gaccgcggcg aaggccttgt cgcgcgtgac cgtgaagcgt ccggagcgga
     5701 tcgtcttgcg cagcgatcgc gacatctgga atttgtgcag cgggatgatc gcgcgccgcc
     5761 gcggctcgac ccagaaaacc tcctgcgcgt tgcggctgtc ggccatcggg aagacgccga
     5821 tcgcataggc gcgcagcagc aggtccggat caagcggggc catcgccccc gcttgtcgcg
     5881 ggcgcaccgc cccgtcaacc gcttccccgc ccgggggccg ccgccgcgct agaggatttc
     5941 ccgcaccaat tcctgcgggc ggcacagccg gacgcccttt tcggtctcga ccagcggccg
     6001 gttgatcagc accgggttgg ccaccatcgc ctccagcagc gcgtcgtcgc tgaccgcgcc
     6061 ttcgactagg tcgagatcct tggcgagctg ctccttcgcc ctcagcccct cgcgcggcga
     6121 aatcccggcg cggtcgtaaa gccgccgcag ctcgtccttg gacggcgggt gcttcagata
     6181 gtcgacgacc gtcacctcgg cgccttcctg ctccaataac tccagcgtct tacgcgacgt
     6241 gccgcatctc ggattgtggt agatggtggc tttcatggct tcgctccctc agccgccgcg
     6301 gtcgagccgc agcagatagc cgcgcttttg cttgaggtcg gtctgcaccg ccagatagat
     6361 tgcgccgtcc gcgtcgaccg tcacccccgc cacctgcgcc agatccccgc cgaacaccgc
     6421 gatcgtcgaa agcccgtcct cgggcgagta aacgtgcagt ttcccgttgg cgctgtcgct
     6481 gatgtagagc gccccgcgcg cataccagat ggtttccggc gagaagccgt cgcggttggc
     6541 gagcggccgg tggaggcttc cgtccggccg cagcaaatag aggacgtcgt cttcattgtc
     6601 ggcgatatag aggttgccgg cctcgtcgaa cgccatgctt tccggcttgg tgattccttt
     6661 caccaccacc gatacctgcc cggtccggct gatgccgagc acgcggtgcc cgtcggcatc
     6721 gcccaccagg atagcgcccg atggggcgag tgcgatccct tcggtggcgc cgagcgcggc
     6781 cgagcggcgg gcgaggagga cggtccggcc ggacgggtcg acgccgcgga cgccgccggc
     6841 ttcgtcgtcg gtgaagaaga cgttgccttg cccgtccacc gccagatcct cgggcgaacg
     6901 cagcccgtcc gccgcattcg ccagcgcgac cgagcggccc ttgccggtcc agacgcggat
     6961 cgcgctgccg ccctcgtcgg ccatgtagag cctgccgttc gcccaccaca ggccgtccgg
     7021 cacggtaaag ccgtcgccgc tcctggcgac gatgctcgcc cgccaggccg ggtcggggag
     7081 caggaccatg ccgtcctcgc ccagcccgca gccgccggcg agcagcgcca gcgccgccgc
     7141 gagcagcttg cgcccggcgc tcatgcggcg cctcccagcg cggcgatggt cttggcggca
     7201 tcgaggatca tccgcacgtc gtcgagcgca ttggtcgcct cgaagacgaa caggaaggcg
     7261 aggctggccg acagcgccgg atgacggatc gtccaggccg tgatcggctc ggtgaggcgg
     7321 cttccccagc ggctcgccag ccattgcagc gcggcgagga tcgccagccc gatcgccgcg
     7381 ccgccggcaa ggtcgagcgg cgaatgctgg ccggtgatca cccggctcgc cagcgtcacc
     7441 gccagcagcc agccgatcgc gaccagcccg gcccctcgcg aggcgagcgc gatgccggcc
     7501 gccagcgtgg cgtagaagcc ggcatggtcg ctggggaagg agccgaggtc gttctgctcg
     7561 atctccccgc gcttgagctc gtcatgctcg gcgccgttgc gctcgttcag cggcacccga
     7621 tagggcagca gcggcgcctg cgtcagcgcg ccgtcgtcga gcagataggt cttctgcgac
     7681 agcacatagg gccgcggcag gaacacgctg gtcgagatca gcttggtcgt ggcgatcgcc
     7741 atcaccgagg ccaaagcggt gacgatcagg atccgccgcc gccgcttgat cgccgcgtca
     7801 tcgcccgcgc cgtgccaggc gaagatgaag caggcgccga tcacgccggc cttgaccagg
     7861 ttcgattcga gcggaagcgc caccagagtg tcgaacaaag gcgagcgtcc ggccagcccg
     7921 ttcagcagct ggtacagccc gcggtcgagc ccggtcagca cgccgcctct ccccaaccag
     7981 ctcctgacga cattctgctc acgcgatacg gcgccccacg aggaaacgag ccatatccgt
     8041 tctcacaagc gcggcgcttt gggaatcggc ttcgtcggcc gcggctccgg ccgctgtgcc
     8101 gcttaaggac agcgtgaagg ccgtcagccg gcttccggca agtggggaag cggcgcatcg
     8161 acctcgcacc gcaccccctc cggcctgaag ctcagcaccg cgcgcccgcc gagctccgcc
     8221 gccagcgccc gctcgatgag ccgggtgccg aaccccgacc gccgcggcgg ctcgaccggc
     8281 ggcccgcctt gctcttccca aaccagattg agccgctcgc cctcggtccg ccaggtgatc
     8341 gccacccgcc cttccgccac cgacagcgcg ccatatttga ccgcgttggt ggtgagctcg
     8401 tggacggcga gcgccagccc caccgccgtc tgcggcggca gcagcaggtc cgggccctcc
     8461 atcgtcacgc ggtcctcgcc cgcgcccggg aagctcgctc catcgcggac gatctgccgg
     8521 atcgacgccg cctgccaatg ctgatcggta agcagattgt gcgcggccga aagcgccgaa
     8581 agccgccctt cgaacgcttc gcgcatcgcc ggcggcagcc ccgcgccctt gaacgattgt
     8641 tgggccagcg cctgcacgat cgccagcgtg ttcttgaccc ggtggttgag ctcgttgacc
     8701 agcagctgct gccgttgctc ggccgcgcgc cgctcggtca cgtcgcggcc gatcgcgaac
     8761 aggccgatca cccggccgtc ggcgtcggcg gcgagccccg aattgatctc ccagtgaagc
     8821 tgccgcccgg tccggctgcg cacctcgata tcatattgcg tgttgccgcc ccgctcgagc
     8881 ttctcgcgga gcctgcgcga ggtctcctca tagccttccg ggctgacgaa ctcggcgatg
     8941 ttgcgcccga tcagcgcctc gcgcggcgcg cccaccgccc gcgccgccgc ctggttgcag
     9001 tcggtgatat tctggtcgag atcggcggtg aagacgatgt cgttcacctg ttcgaacatg
     9061 tcgcggtagc gctgctcgct ccggcctagc gcgcgctcgg tggccagcag ctcgcgcgtc
     9121 accccgcgcg cgcgcccgag cagcacgacg gtggcgcagg cgaccacgaa gaacaggatc
     9181 aaagtgttgc cctgggcggg atcgtcgatc cggaacgaat atcgctccgg aaccaggaaa
     9241 taccagctcg ccagcgcgcc caggatcgcg gccaccaggc cggggccgat cccgtaaaga
     9301 taggcgattg cgatcaccgc cgcgaagaag gtgatatact ggacgcccgc cagatagggg
     9361 tcgagcgcca gccggaaggc gagcgcggcc agcgtgtaca gcaccgccgc ggcgtaggcg
     9421 cgggcggtct gccccgaccc ggcttcgccc ggcgctgccg ccatctagta agccggctcg
     9481 agcaggccct cgatgacgtg gtgctggacc accgtctcga gcgcgtcgac cgttcggtag
     9541 atgagatggt cgagcacccg cggcggcagc gtcgcggcat aagcgcgcgt cacctgcagg
     9601 tcgtgatgct gcgaattggt ggcgtcgggc gcgtcgacgc cgaccaccag caccggccgc
     9661 gccgcgttcg agcggttggc ggtgccgcga tggatggtga gcgccgatcg cgccgccatg
     9721 tcccctcgct gcggcatctt ctgcaccgcc cggccttcat agcgcggcca taattcgcgc
     9781 ggcgggaaca tgcccttgtc gcagccttcg aactggtccc attgcgtccc cagcgcgatc
     9841 tcgaacgggc ccatttccgg caccgtgtcg accgcggtga ggttgaaggc gagcgaattg
     9901 aggcggcggc ctttggtggt ggcttcgggc acgaagaagt cgcggtgcca gggctggtcg
     9961 gcggcgcccg ggaaggggat gtcgaagccg atctcgacga tcttgtaatc gggcccgagc
    10021 accgcttccg ataccgccac gaaccagggg tgggtgacaa tatcgacgaa gccccccacc
    10081 cgctccggct gcacctcgac gtagaaacgc tgcggcccgc gcggcagcgc gccgcccggc
    10141 accgccttgg cttcctcgta aagccgctcg atatcctcgc gcatggcatc ggcccaggcc
    10201 ggcggaaagg cccctttgag gccgatgatc ccgtcgccgt aaagcccgcc catgatcgcc
    10261 gcggtgtcgt agttctgatc gctgctcatc tcacgcctcg ctgctcctgg gcggcagcct
    10321 aaccgtcttc agccgattct gcgagtgctg acatggactt ggccggacca ttgcatgacc
    10381 ttcgccgctg cgcccttgtt cgtcatcccg gcgaaagcgg ggatcccgct tctcctttgt
    10441 tcccagcagc ttgataaaag ccgggattcc cgctttcgcg ggaatgacgg ggtcggaggt
    10501 ccttatgcaa aggtccggct tggcaggctt gctcgtccgt cggtcggggg agcggcggga
    10561 acagcgcatc gggcagggcg tttcacccct gtaacatttt ttcgaggagg ccgagatgga
    10621 agtcgaacgc gagcccgtga cgaccgaaaa ggaaacggtg atcatcgagc gcggcggcgg
    10681 cggcggcgtg atcgccgcga tcgcctttct gatcctggtt ctggccgttc tggcctattt
    10741 cggaatgctg ccgatctaag cgaaggccgg cttcagccgt ccttgcgctt ggggcggttg
    10801 gcgtacagca aggcggccac gatcgccgcc gatccgatcc cgaaggcaag gccggcgcgg
    10861 acccgctgct tgcgccgttt cggaccgccg ccttcagctc cctgctgttc cgccgggggc
    10921 gtggcgcgcg tctcgtctga accggtcggc ggcaaggtca tgctttcact ccagcgtatg
    10981 ggcctcgcgc cataaccgct ttgcccgccc ttcggctagg ggtggcgccc cgcctagcga
    11041 tcaggtcagg atcaggcttc gagccggacc cgcactcgct tcttttcctt gggcgggggc
    11101 ggcaggttcg ggcgctggcc gaccttgcgg tagacgcggg ggccggtatc gagccggaac
    11161 ggccccatct tctcgcccgg cggatcggct tcgcggccga ccaaatagga ggtcgccgtc
    11221 tgcaccctgc cgccgccgcc gtcgccgcag aggaagttga accccaccag cgggatgaac
    11281 aggcgccggc cctgaagctc gatgccgcgt atgtccgcat tcgccatcga agcgacgccc
    11341 tgcagctttt cctcctgccc cggggcgagc cgccattcgc ccccgtccga tcccagcaat
    11401 tgctgctctg cgaagaaagc cgccagctcg gcttcctggc ccgcgccggc gttgaacatg
    11461 tcgatcgcga cgcggatgtc cgccgccgct gcatccccgg cattcctgac cgtcagccgg
    11521 taatggatct cggccttgtc cggggtcagc accacccggt ccggctggaa gtcgagctcg
    11581 atccgcggct gcggcgccct cggcaccgcc acgggcgcgg gcggcggcac cgggcgcggg
    11641 gcaggggccg gcacccgtgc ggcgcgctcg cgcaggtagc cgccgccggc aaaagcgtca
    11701 cgccggcggc gcagcatgac gagggcgagc agcgccagca gcgccgccag cccgaccgcc
    11761 cacggcatcc agccgagcga ctccgacgcg gccgccccgc tgccgggatt ggcgaccgga
    11821 gcgggcaggg ccggagcggg gaagctagag ccggaaaaaa cgggggcggg aagatcggcc
    11881 tccggctgga ccacgtccgg cgcagcggcg gaagtttccg tgcggtcggc gggcggtccg
    11941 gctgccgggg ccgcgttcct cgcgggtgcc ggttccgcgc gggcgggagc ctcacgggca
    12001 ggttcgcggg cgggcgccgg ggccggcctg gtctcggtcc gttcaggcgc gggggggggg
    12061 gcggcgacgg gcggcggcgc tgtggcctgc tcgggcgcgg gggcaggcgt cgttcgcgtg
    12121 ccgggcaatt cgaaattgcg cagctccgga ttgccgaccg tgttcgactc ctgcccctgc
    12181 gcggcggcga caggcgtcag ccataggaac gtggcgagca aaatcctccg gtggcggtac
    12241 atcgagcgga caagttcctg gagaagcgaa acatctcctc tattcccctg cctcggccgc
    12301 gtccacctcg gaagcgggtc aagccgcgcc cgccatggct cgccgcctcc ccggcgcggc
    12361 tcgtccgcac cggccgcacc ggtggcggtc tcgttcgttc ggacggccga atcccaaggg
    12421 agaacgacat gtccaagagc agcgattcga acaaatccaa caccgtcaac agcggcggcg
    12481 cgcccaagcc tactcaggag gagcgcgacc gcgagttcgc cgacatcacc ggcaatgtcc
    12541 ggctgaaggg cgaggacccg tcgcccgacg accgcggcga gaccaccagc cgcgagcccg
    12601 ccccggcgat cgacatcgac aatagctgag gccgccggcg agggcgcggc aggccctcac
    12661 tcgcagcgat gcacctcgat cgtgatgtgg cacaggcttt tgaggtgggc gatccgggcc
    12721 ttgtaggcgc tcgggctttc gggcgcgtgg ctgaccaccg tcaacaccgc cgccatatgg
    12781 cccggcccca cccgccacac atgcaggtcg gggacgaggt cgccgccggt ctcgagcttc
    12841 gcccggatcg cctccgccag ccgcggatcg ccggccgcgt cgagcagcac cgcgcccgaa
    12901 tcgcgcatca acccccacga ccagcgcgcg atcaccagcg cgccgaggat gccgatcgcg
    12961 ggatccatcc agacgaggcc gaaccggctc cccgcgacca gcccggcgat cgccagcacc
    13021 gaggtcagcg catcggccag cacatggaga taggcggcgc gcagattgtg gtcgtgatgg
    13081 tgatgatgat ggtgctggtg gtcgccatgg tcgtgccgtt ggccgccgcc gccatggagc
    13141 agccaggcgc tgacgaggtt cacggccagg ccgaggcagg cgaccgcgat cgcctcgtcg
    13201 aaatcgatca tgaccggctc caccagccgc tcggcgctct cccacccgat caacaaggcg
    13261 gcgatcgcca gcgtcaccgc gctggtgaag ccggcgaggt cgccgacctt gccggtgcca
    13321 aaggcgaagc gcggattgtg gagatgccgc cgggcatagg cgtaggcggc cgcggccagg
    13381 gccagcgcgc cggcatgggt cgccatgtgc agcccgtcgg ccagcagtgc catcgatccg
    13441 aacatcacgc cggcggtgac ttccgcgacc atcatcgcca gcgtcagcgc gatcaccagc
    13501 cgggtgcgaa gctcgttcct ggcatgatgc tcgccgagga aggcgtgatc gtgctgccag
    13561 cggtcgtcgg aatgcgaatg cgtcatcgcg ggcgggggcg gaggggatcg gtcatcaacg
    13621 gcctatagcc tccgaatcgg actggatttc aatcgcctga gcatagccca cgtccaaccg
    13681 gctggaagca ccctccagaa accaccgact attcgcccgc ctccggccct aatagcggga
    13741 gcgaagcgca tgagcgggtc gaaagccgcc gcggcggcgg caaggtcgtg ctcgagctct
    13801 gagggggccg cggaagcgct ccagccgtcg ggatggagcg ctcctcgcgt accccggcag
    13861 cctcaccccg atcgcgcccg cggtcaagcc gccgttgggg ccgcgtctcg ccgcggtacg
    13921 aggggcgttg gaaaacttaa cgccgtctga cgactccatt cagcctttcc taaaccgaat
    13981 cagtgcataa gcgcatttgt cggcaagtgc cggggcatgc gtctggccga gtatgtccaa
    14041 ttcgccccgg tgtttccggc ccctcgtgtc gcgtatcgag ggcggggccg gcattcctta
    14101 gggagtgccg gccccgactt tatttggcct ccgccacccc ccttagccgg gcgccgtcgc
    14161 cttcatccga tccggaacga tttacggctc gccccgttag gcgatttgcg gatggagctt
    14221 gctggcccgc gcgtatcgtc gcgcgtccgg cttgaagcgg ctccggatcc gcctgtatca
    14281 gggaggctcc tatgaaaaag acaatcaccg ccataatcat cgccggcacc gtcgcgctcg
    14341 gcggctgcgc ccgcaattat gaagacgacg gcgcgctgca gggcgtcggc accggcgccg
    14401 cggtcggcgc gctcgccgga gccggcgtcg gcgccgtggt cggcggcatc agcccgatcg
    14461 agggtgcatt ggtcggcgcc gcggtcggcg gcattgccgg cgcgatctgg gccgaccagg
    14521 acggcgacgg ccaggccgac ggctattatc aggaaggcac ctattatccg ggccgcccgc
    14581 ctgccgccgc gtcggcgccg ccttaccagg cgccctatca ggcgccgccc cccgagccgg
    14641 tccgccgctc cggtgagcgg ggccgctaag caacgccccc gatgaaccaa tccaatgtgg
    14701 gcgccggccg gcagcgttgg ccggcgcaga tctcgatccg cgggctgtgc ctcgccgcgg
    14761 ccctcgcggc ggcggcgctg ccggcctatg tgccggcctt cgcgcagggt tctccgggcg
    14821 cggtcgccgc cgcgctcaaa tcctcggcca aatcggaccg cgagctgcgc gccttctatc
    14881 aggcacgcgg ccatcggccg ctctggttcg ccgccggccc cgcctcgccc gatgcggagc
    14941 gggtcgtcga tctgatccga agcgccagcc tcgatgggct cgatcccggc cgctacaagc
    15001 cgaaggcgct ggccgaggcc gttgccaagg cgcgcggcgg ctcgcccaag gcggtcggca
    15061 aggccgacgc gatgctgtcg cgcgccttcg ccgcttatgt ccgcgacgtt cgccgcccgc
    15121 gcaacgtcgg catggtctat gtcgaccgcg agctcgcgcc cgtccagccc ggcgtccgcg
    15181 ccgtgctcga cgccgccgcc gccgcgccgt cgctggccgc ccatctcgac ggcatcgcct
    15241 ggatgaaccc gctttacggc cgcctccgga gcggcctcct cgcctatcgc gagcgctggg
    15301 cgcggctgcc gcaggcggcg atcgcgcccg gccccgtcct gcgcgccgac ggcgaccgcg
    15361 tgcgcatgct tcgccaccgc ctcggccttc ccgccgacgg cagcttcgac gaagggctca
    15421 cctccgcgct ccgctctttc caggccgccc acggccttcc cgacgacgcc gtcgcgggcc
    15481 cacgcacgct cgccgccctc aacgccgacc cgcgcggcca ggagcggctg atccggctca
    15541 acatggagcg cgcccgcggg ctgcccgcca acccggggcg ccgctacatc ctggtcgatg
    15601 ccgcctcggc gaggctctgg gcttatgagg acggccgcgt cgtcgacacg atgcgggtga
    15661 tcgtcggcaa gccgaccgag cagacgccga tgatggccgg cctcattcgc tatgcgatgg
    15721 tcaatcccta ttggaacatc ccgcccgatc tggtccgcaa ccgggtcgcg cccgcggtgc
    15781 tcaaatccgg catctcctac gtcaagtcga agcgctacga agtacgctcc gactgggccg
    15841 aggattcgcc ggtggtgaac ccgaaggacg tcgactgggc cgcggtcgcc gccggccggc
    15901 aggagcttcc ggtccggcaa ttgcccggcc gcgacaatgc catgggcaag atgaagttca
    15961 tgctgcccaa ccagctcggc atctacctcc acgacacgcc cgaaaaggcc ttgttcaagg
    16021 aggaggagcg ccgcttcagc tcaggctgcg tccgcgtcga ggatgcgccg cggctggccc
    16081 gctggctgtt cggcaagccg ctcaaggtgc ccgcgcgcgg gtccgagcgg cgcgtcgatc
    16141 tgccgcgccc ggtgccggtc tacatcacct acctcaccgc cgccccggcc gaacagggca
    16201 tcgccttcag cgccgacgcc tataatcgcg acgctcgcgc cctcggcgcc cgctcgatgg
    16261 cggcgcgctg agcgggcgct accaacctgc agcgttgcag ccgggactca cctgcggcaa
    16321 aaacgccgcg cccccgggtt taccgctggc gcggccgtga cgtttgattc actctcattg
    16381 gcgataccgt tgccgacgag gtgtcgtgtt atgattttga tcgctcttcc gataatgacc
    16441 ggcgccatgc tggccttcat gttcatcgcg accgtctaca agatgccccg cgacctctag
    16501 ccgggcttcg ccgccggcgg cgggcgcctc agccgaagat cgacaggtcg agcggctcgc
    16561 tcgcgccgaa ccagatcacg tgccgtgtgt ggtcggcgag atcgtcgccc gtggtcgcat
    16621 ggacgaacag ggtgagctcg ccgcggctca ccgccgccca ttgcgcgaat tcgccgaacc
    16681 gctcgggcgc caccgtcaat tggcagctgc cgcgcgggtg cggcccgacg gcacgctcgt
    16741 ggaaccgccc caccgacact ccgaaccgct cccgcgcctc cccggcgagg gcctgcgcgg
    16801 ccgccagttc gggcggatcg aaatagatat gggcgtgata gttcaggatc tgcggcatcc
    16861 accccagatc ggcgctcccc gccccggttc aaggtcgcct tgcgttcggg cttgaaccgc
    16921 gccgccggcg cggtgaaggt ttcacatgga tggcccgccc gcacccacca gcaccgccgc
    16981 ccttcgcgcc gggccgcggc ggggcatcgc cgctgcattg ctgacgcgtc cgcgcgcccg
    17041 cgacttcggg cggatggcgc tcgaggccgt ttggctggtg ccgctgctgc tcgcgatcgg
    17101 ctggctcggc gacctgatgc actggcgccc gactttcgac gccaccatcg cccgccttgc
    17161 cctcgtcgca atcgtcgcgc cggcgctggg cgaggagttg ctgttccgcg ccgctttgct
    17221 gcccaatccc gcgcccgccc gctggccgct cggggcgatc gccgcgtcgg tcgccttgtt
    17281 cgtcctctgg catcccattc aggcgctcgt cttcggcccg cattgggcag agacggttct
    17341 cgacccgtgg ttcctcgccg cggtggcggc caccggcatc gcgctggcgc ggctctacct
    17401 ggcgaccggc tcgatctggc cgtgcgtgat cctccactgg ctggtggtgg tcggctggaa
    17461 agccctgctt gccggcccgt cgccctggct gccgtcctga accttcaccg gccggtcgcg
    17521 cagcgctcca tgaaccgttg ccagatcgcc cgcgctgccg cgtccatgga cacggccatc
    17581 gtatagccgg tcgtgaaaag cgagatcatc tcgctcctgc cgaccgcctt cagccgcttg
    17641 tcgtttccgg gcaggcggat ggtgaggtcc cagccgccgg ccggattctt caccgccgcc
    17701 gccggcatga atcctggctc gccggtcccg ccgaacaggc ccatcatcgc cgcgccgggc
    17761 ggcaacactt gcgccccgtc gtcgacctgg cttcgggtct tcacgtccag aagcttggtc
    17821 actccggttt gggtgacgat cacgtcaccg gccgcgcatt cgaaccgata ggagaccggt
    17881 tcgcccaggc caaaggtcag gcgggcgccg cggccgccgt cgccgggctg gagcgcccac
    17941 tcggccgctc cggccggcgc cgacacagcc agcatccgcg cgacaccgaa gactttgacc
    18001 taacgcgaca tgactgctcc ccctccagat acgcgcagcc gagttgcgct gcgatgcccg
    18061 cctccgtcaa gcgtccgccg atgccgcgct tcgttgccgc cgtcggcccc gcccgctaga
    18121 gttcgccgat ggcatgtccg gcggaggcga tcatcggcac ctaccagcgc catgcccgcg
    18181 cctgggaccg cgcccgaagc cgcgcgctcg acgagcggcg ctggctcgac aagttcgccg
    18241 atctactccc ccaaggcgcc caaatcctcg acatcggctg cggcggcggc gagccgctcg
    18301 cccgccactt gatcgagcgc ggatatcggc tggccggcat cgacgcgtcg ccgccgctca
    18361 tcgagacctg cagggaccgc tttcccgatc acgattggca cgtcgccgac atgcgcgagc
    18421 ttgccctcgg ccgccaattc gacggcttgc tggcctggga cagtttcttc cacctcgccc
    18481 ccgccgacca gcgccggatg ttccctatct tccgcggcca cgccgcgccg ggcgccgcct
    18541 tgctcttcac cagcggcccc gcgcatggcg aagcgatcgg aagcttcgag ggcgagccgc
    18601 tctaccatgg cagcctcgat cccgatgagt atcgcgacct gctcgcgcgc agcggcttca
    18661 ctgtcgtcgc ccatcaagtc gaggaccccg agtgcggcca ccatacgctc tggctggcca
    18721 ggatgcaggc cgtggcaata agcgcccccg cgcactagaa gcggacctat gatcgttcgc
    18781 acagcaacct gccgctgcgg ccaattgcgg gcgacctgca cgggcgaacc ggtccgcatc
    18841 tcggtctgcc attgcctcgc atgccagaag cgcagcggca gcgcgttcgc ggtccaggcg
    18901 cgctggcccg acgatcgcgt ggagctggcc ggcgagcctt cctcctggtc gcaaacgggc
    18961 gacagcggct cgctcgccac gttccttttc tgcgccaatt gcggcgccac gctcgcttac
    19021 gccagcgacg ccatgcccgg tctcaccgcg atagcggtcg gagccttcgc cgacccgaat
    19081 ttcccgccac ccgaatattc ggtgtgggag gtgcgcaaac atggctgggt cgcgataacg
    19141 ggcgaaggca tcgagcattt cgaatagcgg acgcgggcaa aagccgtatt tgcagcaaca
    19201 taggccttgc gccggcgggg aagatcgctt gctccacctt gccccaaggc cgctcgccgg
    19261 ctccggcggc aattaacgat ttgccgcctg ctgtcccggc gcgagacgcc ccgaaacggc
    19321 cgcttgcatt caccgccatc tggcgcaggg ggaaggcaag aaccggatgt agcggttctt
    19381 ctgtaaacag gagtacccct tatgactctc accaaatcct ttgcgatcgc ggcggcgatg
    19441 ctgctctcca cggcggctaa cgcaggcacg atcaacgtca ccccgtccga tatcggcaat
    19501 tccttcaccg tcggcttcga cggcttcgcc aacgattcgg tgatcgacgg cctcagcgcc
    19561 gaggcgacct tcacgctcac cgaagcgacc ggttccagct acaccttcga ctatgacatc
    19621 gccaacacgt cgggcggcgc gatcgacgcc tcgcgcgtct cgatcttcgg cttcaatacc
    19681 gatccgacca tctcgggcgc gacctcgacc ggcctgttca gcgaggtcaa caccaacgcc
    19741 aacgttccga gtggcttcgg ccaggtcgac gtctgcttca agggctccgg cgacggcaat
    19801 agctgctcgg gcggcggcgg caccggcgtc tggatgggcg acagcaccag cggctccttc
    19861 accctgacct tcgaccaggc g
//
LOCUS       DASVRK010000224         4629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_355462, whole genome shotgun sequence.
ACCESSION   DASVRK010000224 DASVRK010000000
VERSION     DASVRK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355462"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..924
                     /locus_tag="VF589_08940"
     CDS             184..924
                     /locus_tag="VF589_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257748.1"
                     /translation="MKAAACGLALALALVAAPIRAARIEPASGAAVTTFATTFPVSLE
                     REIDGWMLGAYADWTTRPPGPPPLRRPLAGPRRESQSAAFRAAGLSDLDVSAMKSFSI
                     GRRLSLDLAAELRLPTGSERDGLGAGRTEAMIDAGLSTTAGRWELWTGAARRLRGASE
                     LHSGRDVWDIYGGAEGRVGARATLRYEIERRQAAYSGGPAELSARLSLRRKLARGISL
                     TFAAASESDEFGSERLATVALRWQLRPH"
     gene            936..1949
                     /locus_tag="VF589_08945"
     CDS             936..1949
                     /locus_tag="VF589_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX8257749.1"
                     /translation="MQVYHAAAAGVLVLAGAAPAHAEWRHEVGEVSIVANGGVSLLGA
                     LVDEPDEPGGFEADGDIDAYGSLNLEWTSNSGLILGGFGSGDTADNRPDTLKNDRLYT
                     YVASEWGRAEVGLTSGPARRMTFYAPVVGSGQVRGDFARYAGRSALLWPVDTRQSFKF
                     AYFSPPIGNVRFGASWSPKVERFDAVQRNAFELGAQYEQPVGDWVLGASAAYVHGDAD
                     TPGLKDINSWSLGFQARRGKLVIGGAYVDRGDSNLFIEDFGQSEVNLGVAWRATGWAV
                     ALSAARTTSELIDNDLVGLGGTYDVGRHVTLTSDVVVMREQDTLGDTRSGLVWVAGVD
                     LKI"
     gene            2038..2484
                     /locus_tag="VF589_08950"
     CDS             2038..2484
                     /locus_tag="VF589_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257750.1"
                     /translation="MAYLEGNAGNNTIRGTANADNIEGKGGNDRLYGLGGNDDLEGDA
                     GDDWLFGGAGNDILEGGLGNDRLSGGGGRDIFEMDDPVTGRDLITDFQDGLDRIKVEF
                     EGDENFSDLRIREDANGNAIVAWDGGSVKLVGIGSEQLSAADFIFG"
     gene            complement(2800..3543)
                     /locus_tag="VF589_08955"
     CDS             complement(2800..3543)
                     /locus_tag="VF589_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257751.1"
                     /translation="MTISDETLFAWLDGTLPPDEQQRIALIIAEDPALAERVERQAQL
                     AEAARDSFAADLDEPIPDAWIAAIDAALPQAQAGQVASLAAAREKKRARWTSWQAGGA
                     IAAALVLGLFLGNGASGPGGDLVVEQGGALMAAAEVGDALDGARSGVPVQLAGGRSLD
                     VQISLRTPQGGFCREALLSAPGGSDRMLACREDQGWRIAGLAHEAAGGTGYETVSGEG
                     ALDGLVDRLGAEPLDAAAEQAAIQRGWKR"
     gene            complement(3540..4043)
                     /locus_tag="VF589_08960"
     CDS             complement(3540..4043)
                     /locus_tag="VF589_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8257752.1"
                     /translation="MTGELTPKLREELIAFLPRLRRFTRSLAGGNAEAGDDLAQAAIA
                     RALANIGGFEEGTRLDSWMYRIAQNLWIDSRRSERTRGIQVSDEALAYVAGDDGRRVT
                     EARLTLEATRVAIEALPDEQRAAVMLVLVDGLSYQEASASLSIPIGTIMSRIHRARAT
                     LEAKVFG"
     gene            4176..>4629
                     /locus_tag="VF589_08965"
     CDS             4176..>4629
                     /locus_tag="VF589_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257753.1"
                     /translation="MKTGRTMTAALALMLALAAAADARRGRGGSSGGSSDSGGGDVEA
                     VVDDSRSSFDADDRADAAEDAADDRADAAEDVADDRGDAAEDAADDAADAQTETADDR
                     ADAAEDAADDAADAQEDAADDRADAQEDAADDAADAQEDAADDRADAAE"
BASE COUNT          683 a         1538 c         1727 g          681 t
ORIGIN      
        1 cggggacgcg ggcacggcgt cgacagcgac gacgatgcga gccaaggcgg ccagggccgc
       61 gggcgcgggc gcggtcgcgg cggccgggcc ggcgaagctc ccagcaccga cgattgagcc
      121 gaagggtcgg ggcggcggcg atgggtcgcc gctccggtca ttccccgatc cggagccgat
      181 ccgatgaaag ctgcagcttg cggccttgcc ctcgcgctgg cgctcgtggc cgcgccgatc
      241 cgcgccgcgc ggatcgagcc ggcgtcgggc gcggcggtca ccaccttcgc caccaccttt
      301 cccgtttcgc tggagcgcga gatcgacggc tggatgctcg gcgcctatgc cgattggacg
      361 acgcgcccgc ccgggccgcc gccgctgcgc cgcccgctcg ccgggccgcg gcgcgagtcg
      421 caaagcgccg ccttccgtgc cgccggcctg tccgacctcg acgtgtcggc gatgaaatcc
      481 ttttcgatcg ggcggcggct gtcgctcgac cttgccgccg agctcaggct gccgaccggg
      541 tcggagcgcg atgggctcgg cgccgggcgc accgaggcga tgatcgatgc gggcctcagc
      601 accacggccg ggcggtggga gctgtggacg ggcgcggcgc ggcggctgcg cggcgcttcc
      661 gagctccatt cgggccgtga cgtctgggac atctatggcg gcgccgaggg gcgggtcggc
      721 gcgcgcgcga cgctgcgata cgagatcgag cggcgccagg cggcttatag cggcgggccg
      781 gcggagctgt cggcgcggct cagccttcgg aggaagctcg cgcgcggcat cagcctcacc
      841 ttcgcggcgg ccagcgaaag cgacgagttc ggaagcgagc ggcttgccac cgtggcgctc
      901 cgctggcagt tacggcctca ttgaaggggg gaattatgca agtttatcat gcggctgcgg
      961 ctggcgtgct ggtgctggcg ggggccgcgc cggcgcatgc cgaatggcgg cacgaggtcg
     1021 gcgaagtctc gatcgtcgcc aatggcggcg tgtcgctgct cggggcgctg gtcgacgagc
     1081 cggacgagcc tggcggcttc gaggcggacg gcgatatcga cgcctacgga tcgctcaacc
     1141 tcgaatggac atcgaattcc ggcctcatcc tcggcgggtt cggatcgggc gacaccgccg
     1201 acaaccggcc cgacacgctc aagaacgacc ggctctacac ctatgtcgcg agcgaatggg
     1261 ggcgggccga agtggggctg acgagcgggc cggcgcggcg gatgaccttc tatgcgccgg
     1321 tcgtgggatc gggccaggtg cgcggcgatt tcgcccgcta tgccggccgc agcgcgctgc
     1381 tgtggccggt cgacacgcgg caatcgttca agttcgctta tttctcgccg ccgatcggca
     1441 acgtccgctt cggcgcctcc tggtcgccca aggtcgagcg gttcgacgcg gttcagagga
     1501 acgcgttcga gctcggcgcg caatatgagc agccggtcgg cgactgggtg ctgggcgcga
     1561 gcgcggccta cgtccatggc gacgccgaca cgcccgggct caaggacatc aacagctgga
     1621 gcctcggctt ccaggcgcgg cgcggcaagt tggtgatcgg cggcgcttat gtggaccgcg
     1681 gcgacagcaa tttgttcatc gaggatttcg gccagtccga ggtcaatctc ggcgtcgcct
     1741 ggcgcgcgac cggttgggcg gtggcgctgt cggcggcgcg cacgacctcg gagctgatcg
     1801 acaacgatct cgtcgggctc ggcgggacct acgatgtcgg ccgccacgtc acgctgacca
     1861 gcgacgtggt ggtgatgcgc gagcaggata cgctcggcga cacccgctcg ggcctcgttt
     1921 gggtggccgg agtcgacctc aagatctgaa gccgaaaaaa tcgtcggcgg tgcggaataa
     1981 gcgttcgcga atcccgttgg aacaatggct tcagcgctaa caaagggaga cacgaacatg
     2041 gcatatctgg aaggcaatgc gggcaacaac accatccgcg gcaccgcgaa cgcggacaac
     2101 atcgaaggca agggcggcaa cgaccggctc tacggcctcg gcggcaatga cgacctcgaa
     2161 ggcgatgccg gcgacgactg gctgttcggc ggcgccggca acgacatcct cgaaggcggc
     2221 ctcggcaacg accgtttgag cggcggcggc gggcgcgaca ttttcgagat ggacgatccg
     2281 gtcaccggcc gcgatctcat caccgacttc caggacgggc tcgacaggat caaggtcgag
     2341 ttcgagggcg acgagaattt cagcgacctt cgcatccgcg aggatgccaa cggcaatgcc
     2401 atcgtcgcct gggacggcgg ctcggtgaag ctggtcggca tcggctccga gcagctgtcg
     2461 gcggctgatt tcatcttcgg ctgaggtaag ccggagcggg cctatggcgc ccccgctgcc
     2521 caggaggcgg cgggggcgtt tttttgtggg tcgggtcggg acggttgtag cggttgcagt
     2581 tccgttcagg ctgagcctgt cgaagccctt tctttcttat tcacggcaag aaggaaggcc
     2641 cccttcgacg ccgcctgcgg cgtcgctcag gacaggcttc gacaggctca gggcgaacgg
     2701 gtggtggggg tttgggttac tgcccgcaaa ggccgggggc ccagaggggg ttggggctgg
     2761 accccggcct tcgccgggga acgactcccg ttcgacgcgc tagcgcttcc agccccgctg
     2821 gatggcggct tgctcggcgg cggcgtcgag gggttcggcg ccgaggcggt cgaccaggcc
     2881 gtcgagggcg ccttcgccgc taacggtctc gtagccggtg ccgcccgcgg cttcgtgggc
     2941 gaggccggcg atgcgccagc cctgatcctc gcggcaggcg agcatgcggt cgctgccgcc
     3001 gggggccgac agcaaagcct cgcggcagaa gccgccctgg ggcgtccgga ggctgatctg
     3061 gacgtcgagc gagcggccgc cggcgagctg gaccgggacg ccgctgcggg cgccgtcgag
     3121 cgcgtcgccc acttcagccg ccgccatcag cgcgccgccc tgctcgacga cgaggtcgcc
     3181 gccggggccg ctcgcgccgt tgccgaggaa caggcccagc accagggccg cggcgatcgc
     3241 gccgccggcc tgccagctgg tccatcgcgc gcgcttcttc tcgcgagcgg cggcgaggct
     3301 ggcgacctgc ccggcctgcg cctgcggcag ggcggcgtcg atggccgcga tccaggcgtc
     3361 gggaatcggt tcgtcgagat cggcggcgaa gctgtcgcgg gcggcctcgg cgagctgcgc
     3421 ctggcgttcc acccgctccg ccagcgccgg atcctcggcg atgatgaggg cgatgcgctg
     3481 ctgctcgtcg ggcggaagcg tgccgtcgag ccaggcgaac agggtttcgt cgctgatcgt
     3541 catccgaaca ccttcgcttc cagcgtcgcc cgcgcccggt ggatgcggct catgatcgtg
     3601 ccgatcggga tggagaggga ggccgaggct tcctggtagg aaaggccgtc gaccaggacc
     3661 agcatcaccg ccgcgcgctg ctcgtcgggc agcgcctcga tcgccacgcg ggtcgcctcg
     3721 agcgtcagcc gcgcctcggt gacgcggcgg ccgtcatcgc cggcgacata ggccagcgcc
     3781 tcgtcggaga cctggatgcc gcgcgtccgc tccgagcggc gcgagtcgat ccagagattt
     3841 tgcgctatcc tgtacatcca gctgtccagc ctcgttcctt cctcgaaccc gccgatattc
     3901 gcgagggcac gggcgatcgc cgcctgggcg aggtcgtccc ccgcttcggc atttccgccc
     3961 gccagcgagc gcgtgaagcg ccgcagccgg ggcaggaagg cgatcaactc ctcgcgtagc
     4021 ttcggtgtca gttccccggt cattcgtgat cgtcacccta taacggagcc ccgccaagct
     4081 tattccgcgc cccggaatat tccctagccg ccgccgttct gttttggaat tgatagagaa
     4141 tgctgtagaa agagcaggtt ggtggggatc gaaccatgaa gactggcagg acaatgacgg
     4201 cggcgctggc gctgatgctc gcgcttgcgg cggcggcgga cgcgcggcgc gggcgcggcg
     4261 gcagcagcgg cggcagcagc gattcgggcg gcggcgacgt cgaggcggta gtcgacgatt
     4321 cgcgctcgag cttcgatgcc gacgaccgcg ccgacgcggc cgaggatgcg gcggacgatc
     4381 gcgcggatgc ggccgaggat gtggcggacg atcgcggcga cgcggccgag gacgccgccg
     4441 acgatgctgc cgacgcccaa accgagacgg ctgacgaccg cgccgacgcc gccgaagatg
     4501 cggccgacga cgccgccgat gcgcaggaag atgcggccga cgaccgcgcc gacgcccagg
     4561 aagatgccgc cgacgacgct gccgacgcgc aggaggatgc cgccgacgac cgcgccgacg
     4621 ccgccgagg
//
LOCUS       DASVRK010000225        11449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_419297, whole genome shotgun sequence.
ACCESSION   DASVRK010000225 DASVRK010000000
VERSION     DASVRK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11449
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419297"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="rsmA"
                     /locus_tag="VF589_08970"
     CDS             complement(<1..661)
                     /gene="rsmA"
                     /locus_tag="VF589_08970"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX8257754.1"
                     /translation="MAALPPLREVIARHGLSASKALGQNFLLDGQLLARIARIPGPLE
                     GERVYEVGPGPGGLTRALIEAGAKVTAVERDRRCFPALAEVAAAAEGKLEIVEGDALA
                     VDEAALAGRGAHVVANLPYNVGTALLVRWLGGEAWPPWWRSLTRMFQLEVAERIVAKP
                     DTGAYGRLAVLAQWRSEPKLAMKVHRSAFVPPPKVMSAVVHIVPKATPQGVRAATLER
                     LT"
     gene            complement(661..1644)
                     /gene="pdxA"
                     /locus_tag="VF589_08975"
     CDS             complement(661..1644)
                     /gene="pdxA"
                     /locus_tag="VF589_08975"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX8257755.1"
                     /translation="MPLAVALGDPAGVGPEIIAKSWERRQAADLPAFFAVGDADAVAA
                     VWRGPVQPISDPSEAAGCFAEALPVIRVSAGSPIDPGRPDMEGARNSLDALELAVGLA
                     RSGAAAGLVTGPVSKSQLYAIGFNHAGQTEFVAERCGVSSELVAMMLVGPTLRTVPVT
                     VHTAFRDVPDVLTTEAIVAKARATIRGLQRQFGIEQPRIAVAGLNPHAGEDGALGREE
                     IEVIAPAIERLREDGFDVTGPYAADTMFHAKARSRYDAALCMYHDQALVPVKTLHFDE
                     GVNMTLGLPIVRTSPDHGTAFGIAGKGVADPGAMIAALKTASACARRIAQA"
     gene            complement(1663..3003)
                     /locus_tag="VF589_08980"
     CDS             complement(1663..3003)
                     /locus_tag="VF589_08980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8257756.1"
                     /translation="MKLGTLGRAALLAAGSVAAVAAVAQSAGDPGSSATNLNIPANPE
                     FFGTADPNVVKATAIVNGEVITATDIDQRLALIALANGGRIPADELPRLRAQVLRNLV
                     DETLQIQAAAASEIKVEQKEIDTYFAQYSKNFNQTPESFVAYLRAQGSSDRSIKRQIH
                     GEMAWTRLQRRQIEPFVNVGEEEVQTIIERLKASKGTQEYRIGEIFLPSTPETAAEAQ
                     ANATRIVQQLRGGASFVAYARQFSEASTAAVGGDLGWVRAEQLPEPLAVAARALPVGR
                     VSDPIAVPGGFSIVAVQDTRQVLTTDPRDAVLSLKQLSVSFAQGTSREQAGAKVEALA
                     KTSQTMGGCGGAEQAASTLGAEVVSSDQVKVRDLPPALQEIMLKLNIGQSTNPFGSFE
                     QGVRVLVLCGRDDPVATDGPSFDQVYAQLNEERVNRRARRYLRDLRRDAVVDYR"
     gene            complement(3059..5266)
                     /gene="lptD"
                     /locus_tag="VF589_08985"
     CDS             complement(3059..5266)
                     /gene="lptD"
                     /locus_tag="VF589_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX8257757.1"
                     /translation="MKTLNFLCRTALPLLLCAPGILHAQETADPAAPAAERQVDFSSD
                     ELVYDTSVEVVTATGNVRMTSEGNNLRADRVIWNRQSGEVRAEGNVRIVNPQGDAAYG
                     DSIVLTDTLRDGMVQNLLLVLEDGGRLAADRGERREGYTTLFRAAYSPCAVVDENGCP
                     KDPTWTITAVQVVHDPVENRIRYQGASLNLFGTPIVALPGLSHPDGSQGGGSGLLVPD
                     ISYSRSNGFELAVPYYLKLAPNRDATITPHLYTGVLPMIEGRYRQLTELGAFQLGGFL
                     TYGSRLPLDDPEFGRDRGIRAYLEGNGKFQLDPLWSVTASGRYVTDRTFLQRYDISND
                     DRLRSVVEAERIDENSYISIAGWAFQGLRVTDRAGQQPVALPAIDARWRLEDPVWDGR
                     IEVQANSLSILRTEGQDTQRAFASARWDRRSITPWGQELTLTAYGRGDIYHANDTELT
                     TARIYRGDEGWNGRFIGALAADVRWPLIGEFMGGIQRLTPRIQFVASPPTPNLDIPNE
                     DARAVDLEDSNLFALNRFPGYDRWEDGARVTYGAEWALDLPRVAVSTVVGQSYRLDQD
                     EEIFPAGTGLSDRWSDFVGRTSVKIGRRVDLVHRFRLDKDNFAVRRNELDAVVGGQQT
                     YAMIGYLRLNRDIDPTIEDLRDREELRLGGRVQVAGYWSLFGSAVVDLTNRDEDPASE
                     ADGYEPVRHRLGVAYDDDCLEVGVTWRRDYETTGDFRRGNTFLFRVALKNIGR"
     gene            5440..6921
                     /locus_tag="VF589_08990"
     CDS             5440..6921
                     /locus_tag="VF589_08990"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX8257758.1"
                     /translation="MKVSFATQRPSGAYALAIPVWSEDTLAARLAGLDDGARTLATRA
                     ADAQRFEREAAVIAETFVTEGDMARRLLLVGLGGRRDDDAIYEKAGGALTAKLLTSGE
                     TRLVIDLDGLGLDGEAAARIAFGAAARGWRYDIYRTKLGKKSKPTLEEIVIVGAEGDA
                     DGEWQRKAALFEGLALTRELVTEPANIIYPETFVERCRAALDGTGVEIEVMDENRMRE
                     LGMGALLGVAQGSVRPPRLLAMRWNGAADAAARPVVLVGKGITFDTGGISIKPSLGME
                     AMKWDMGGAGAVAGTMKALATRKARAYVVGICVLAENMPDGNAQRPGDVVTSMSGQTI
                     EVINTDAEGRLVLADAMTWAQREHKPEVMIDLATLTGAMIISLGHEYAGLFSNDDGLA
                     GKLVAAGQASGDKLWRMPLGDVYDKLMDSAIADMKNSGPREGGSITAATFLQRFVDEG
                     VKWAHLDIAGMVWANKAGHLYDKGATGYGVALLDRFVADNYEG"
     gene            6976..7410
                     /locus_tag="VF589_08995"
     CDS             6976..7410
                     /locus_tag="VF589_08995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX8257759.1"
                     /translation="MLVDFYHLATTPLERVLPSICEKVLAGGERLLVVAEADLIGQLD
                     TLLWSYAPGSFLPHGRAGGPAPGEQPVLLSEDPEPLNGATNIALADGRWRDEALAFAR
                     AFYFFDAGQLDAARASWRALKDKPEVERRYWKQDGGKWVQGP"
     gene            complement(7407..8486)
                     /locus_tag="VF589_09000"
     CDS             complement(7407..8486)
                     /locus_tag="VF589_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEX8257760.1"
                     /translation="MRVRAWALALLAAGAALAAQPAGRPGIAYPEVKPGAELRFPRDH
                     GAHPDYRTEWWYFTGWLETDAGPLGFQITFFRSRPAVDPANPSAFAPRQIVFAHAALS
                     DPTSRRLLHDQRAARAGFGLADAGTGDAAVRLLDWSLVRRPDGAFQARIPAKGFTLDL
                     VLRPTQRTLPNGAGGYSRKGPAPSQASYYYSMPHLAASGSVTRGGRRVPVRGSAWLDR
                     EWSSAPLPPEAVGWDWAGINLDGGGALMAFQVRGKDGRALYAGGTLRRPNGSRLALGP
                     GEVRFVPRRRWRSPATGATYPVEADFVVRLPEGERRFALRPLFDAQELDGRGGGMPAY
                     WEGAVTTTGGRGYLELTGYAAPLTL"
     gene            complement(8486..11032)
                     /locus_tag="VF589_09005"
     CDS             complement(8486..11032)
                     /locus_tag="VF589_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8257761.1"
                     /translation="MAERAAAGGAWRWLIAGEWRAFPLRFLLAGLAIAVGVALAFTVH
                     VINRSAADAFGEAVRSVSGDADLQVRAAGGTGFDERLYPKLFELAAVADASPVVQLGA
                     TIGKERVALLGIDVLRALAVTPNLLGRRLTGTDGGTSSRPAMSDAAFDMTALYLSETA
                     QARTGLRPGQPAVVQVGPNSHVFIVAGDLPGVPAGQVVAMVDIAAAQWRFGRLGRLDR
                     VDLKLADGLGEAAARREIAAALPAGAVLSDAENESRRGDSLSRAYRVNLDMLALVALL
                     TGGFLVYSAQSLSVARRLRQFALLRTLGLQERALRAQLIGEGALLGIAGSLAGLALGY
                     GLAALAMRFVGGDLGGGYFGGASPDLHFAPAAAFLFLGFGILTAIGGSYAPARQAARI
                     APADALKNAGDIVDPRRRPAWIPAAILLLMGAGAAFLPAVGRLPVFGYAAVGLVLAGG
                     VAAMPWLARHLLRPLARFDSKLPPFELALRRLLGAPSQAAVALGGIVASTGLMIAMAV
                     MVTSFRGSVDEWLDSFLSADLYVAAGGEQDLFDPELQARLAGAPGVASIAFSKAVRLT
                     LDPALPSVELIVRPVGGPGYSLPLIGEAPARGRGTAFWYSEPAARLYGVEAGDSVRLP
                     IGGGADGYVAGVWSDYVRQQGAIVIDSGDYAALTGDRLRSEAAVELAPGASAAKVGEA
                     IRRRLPPGIAGRVQLAEPETLRRLALSLFDRSFAITYVLEAIAILIGLAGVAATMSAQ
                     TIARIREFGMLRHIGMSPRAIVAMLAGEGALLGLIGIAAGTALGLAISQILIHVINPQ
                     SFNWTMETRLPWGLLLGVAGALVLTSAGTAVLAGRRAVSTDAVRAVREDW"
     gene            complement(11025..>11449)
                     /locus_tag="VF589_09010"
     CDS             complement(11025..>11449)
                     /locus_tag="VF589_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8257762.1"
                     /translation="IGFVFQAFHILPHLTLAQNVALPLVLQRQPRAAALDEAAALLSA
                     VGLGDRLRSYPKELSGGELQRVAIARALVHRPALILADEPTGNLDPETADQILNLLTG
                     AVKQRGAAMLLVTHSPAAAGRADRRLLLTRHGLVASDG"
BASE COUNT         1774 a         4062 c         4012 g         1601 t
ORIGIN      
        1 cggtgaggcg ttccagagtc gccgcgcgca cgccctgcgg cgtcgccttc ggaacgatgt
       61 ggaccaccgc cgacatcacc ttgggtggcg ggacgaaggc ggagcggtgg accttcatcg
      121 cgagcttcgg ctcggaccgc cactgggcga ggacggcgag gcggccatag gcgccggtgt
      181 cgggcttcgc gacgatccgc tcggcgactt ccagctggaa catgcgggtg aggctgcgcc
      241 accagggcgg ccaggcctcg ccgccgagcc agcggacgag caacgcggtg ccgacattat
      301 agggaagatt ggcgacgaca tgggcgccgc gcccggcgag cgcggcctcg tccaccgcga
      361 gcgcgtcccc ctccacgatt tcgagcttgc cctccgccgc tgccgccact tcggccagcg
      421 ccggaaagca gcgccggtcg cgctcgaccg cggtcacctt tgccccggcc tcgatcagcg
      481 cccgcgtcag gccgccgggg cccgggccga cctcatagac gcgctcgccc tccagcggac
      541 cgggaatgcg ggcgatgcgg gccagcagct ggccgtcgag caggaaattc tggccgagcg
      601 ccttcgaggc gctgaggcca tgccgcgcga tcacttcgcg cagcggcggc agcgccgcga
      661 tcacgcctgc gcgatgcggc gggcgcaggc ggacgccgtc ttcagcgccg cgatcatcgc
      721 cccgggatcg gccacgccct tgccggcgat gccgaacgcg gtgccgtggt cgggcgaggt
      781 gcggacgatc ggcaggccca gcgtcatgtt gaccccttcg tcgaaatgca gcgtcttcac
      841 cggcaccagc gcctgatcgt gatacatgca cagcgcggca tcgtagcggc tccgcgcctt
      901 ggcgtggaac atggtgtcgg cggcataggg gccggtgacg tcgaacccgt cctcgcgcaa
      961 tcgctcgatc gccggcgcga tcacctcgat ctcctcgcgg cccagcgcgc cgtcctcgcc
     1021 ggcatgcggg ttgaggccgg cgacggcgat cctcggttgc tcgatgccga actggcgctg
     1081 cagcccgcgg atcgtcgccc gcgccttggc gacgatcgct tcggtcgtca gcacgtccgg
     1141 cacgtcgcga aacgcggtgt ggaccgtgac cggcacggtg cgcaaagtcg gcccgaccag
     1201 catcatcgcg accagctcgc tcgagacgcc gcagcgctcg gccacgaact cggtctggcc
     1261 ggcatggttg aagccgatcg cgtaaagctg cgacttggag acgggcccgg tgaccagccc
     1321 ggcggcggcg ccggagcggg cgaggccgac ggcgagctcg agcgcgtcga gcgaattgcg
     1381 cgctccctcc atgtcggggc ggcctgggtc gatcgggctg ccggcgctga cccggatgac
     1441 cggcagcgcc tcggcgaagc agccggccgc ctcggacggg tccgagatcg gctggaccgg
     1501 gccgcgccac accgccgcca ccgcatcggc atcgccgacg gcgaagaagg ccggcaggtc
     1561 tgccgcctgc cgccgctccc atgacttggc gatgatctca ggtccgacgc ccgccggatc
     1621 tcccagcgcg actgcaagcg gcatcggcaa gatcagtctc tcctagcgat aatcgaccac
     1681 cgcgtcgcgg cgaagatcac gcagatagcg gcgcgcgcgc cgattgacgc gctcttcgtt
     1741 caattgcgca tagacctggt cgaacgaggg tccgtcggtc gccacgggat cgtcgcggcc
     1801 gcacagcacc agtacgcgca cgccctgctc gaacgagccg aacgggttgg tcgattggcc
     1861 gatgttgagc ttcagcatga tctcctggag cgccggcggc agatcgcgaa ccttgacctg
     1921 gtcgctcgag accacttcgg cgccgagcgt cgaggccgct tgctcggcac cgccgcagcc
     1981 gcccatggtc tggctggtct tggcaagcgc ctcgaccttg gcgccggcct gctcgcgcga
     2041 agtgccctgc gcgaagctca ccgagagctg cttgaggctc agcaccgcgt cgcgcggatc
     2101 ggtcgtcagc acctggcgcg tgtcctgcac ggccacgatc gagaagccgc ccggaaccgc
     2161 gatcggatcg ctcacccggc ccaccggcag ggcgcgcgcc gcgacggcga gcggctcggg
     2221 cagctgctcg gcacggaccc agccgagatc gccgccgacg gcggccgtcg aggcttccga
     2281 gaattgccgg gcataggcca cgaacgaagc gccgccgcga agctgctgca cgatccgggt
     2341 cgcattggcc tgcgcctcgg ccgccgtttc cggggtggag ggaaggaaga tctcgccgat
     2401 gcgatattcc tgggtgccct tcgacgcctt caggcgctcg atgatggtct ggacctcttc
     2461 ttcgccgaca ttgacgaacg gttcgatctg gcggcgctgg agtcgagtcc aggccatctc
     2521 gccgtggatc tggcgcttga tcgaccggtc ggacgagccc tgcgcgcgaa gataggcgac
     2581 gaagctctcc ggcgtctgat tgaagttctt cgaatattgc gcgaaataag tgtcgatctc
     2641 tttttgctcg accttgatct cgctggcggc ggcggcctgg atctgcagcg tttcgtcgac
     2701 caggttgcgc agcacctgcg cccgcagccg cggcagctcg tcggcgggga tgcggccgcc
     2761 attggcgagc gcgatcagcg ccagccgctg gtcgatgtcg gtcgcggtga tcacctcgcc
     2821 attgacgatc gcggtcgcct tgacgacgtt cggatcggcc gtgccgaaga attccggatt
     2881 ggcgggaatg ttgaggttgg tggcggagct gcctggatcg cccgcgctct gcgccaccgc
     2941 ggccacggcc gcgaccgatc cggccgccag caaagctgcc ctccccaaag tgccgagctt
     3001 catcattcct ccaataaaag caggcatttc aactgcccca aatatgctga acggcagctt
     3061 agcgcccgat gttcttgagc gccacccgga acaggaaagt gttgccgcgc cggaagtccc
     3121 cggtggtctc ataatcgcgc cgccaggtca cccctacctc aaggcaatcg tcgtcatagg
     3181 caacccccaa tcggtgccgc accggctcat agccgtcggc ctcggaagcc ggatcctcat
     3241 cccgattggt gaggtcgacg accgccgacc cgaacagcga ccaatagccc gccacctgaa
     3301 cacggccgcc gagcctcagc tcctcgcggt cgcgcaggtc ctcgatagtc ggatcgatgt
     3361 cgcggttgag gcggagatag ccgatcatcg cataggtctg ctggccgccg acgacggcgt
     3421 cgagctcgtt gcggcgcacc gcgaaattgt ccttgtcgag ccggaagcgg tggacgaggt
     3481 cgacgcggcg cccgatcttg acgctggtgc ggccgacgaa gtcggaccag cggtcggaaa
     3541 ggccggtgcc ggccgggaag atctcctcgt cctggtcgag ccggtagctt tggccgacga
     3601 ctgtgctgac cgcgacgcgg ggcaggtcga gcgcccattc cgcgccatag gtgacgcggg
     3661 cgccgtcctc ccaccggtca taaccgggga agcggttgag cgcgaagagg ttcgaatcct
     3721 cgaggtcgac cgcccgcgca tcctcattgg gaatgtcgag attgggcgtg ggcggcgagg
     3781 cgacgaactg gatgcgcggg gtcagccgct ggatgccgcc catgaactcg ccgatcagcg
     3841 gccagcgcac gtccgccgcc agcgcgccga tgaagcggcc gttccagccc tcgtcgccgc
     3901 gatagatgcg ggcggtggtg agctcggtgt cgttggcgtg gtagatgtcg ccgcggccat
     3961 aagcggtgag cgtcagctcc tgtccccagg gcgtgatgct gcgccggtcc cagcgcgcgc
     4021 tcgcgaaggc gcgttgggtg tcttgaccct cggtgcggag gatcgagagg ctgttcgcct
     4081 gcacttcgat gcggccgtcc cagaccggat cctcgagccg ccagcgcgcg tcgatggcgg
     4141 gtagggcgac cggctgctgg ccggcgcggt cggtgacgcg caggccctgg aacgcccagc
     4201 cggcgatcga gatatagctg ttctcgtcga tccgctccgc ctcgacgacg ctgcgcagcc
     4261 ggtcgtcgtt ggaaatgtcg tagcgctgga gaaaggtgcg gtcggtgacg tagcggcccg
     4321 acgccgtcac gctccacagc gggtcgagct ggaacttgcc attgccttcg agataggcgc
     4381 ggatgccgcg gtcgcggccg aattcggggt cgtcgagcgg cagccgcgag ccgtaggtca
     4441 ggaagccacc aagctggaac gcgccgagct cggtgagctg gcggtagcgg ccctcgatca
     4501 tcggcagcac cccggtatag aggtgcggcg tgatggtggc gtcgcggttg ggcgcgagct
     4561 ttagataata aggaacggcg agctcgaacc cgttgctgcg gctgtagctg atgtccggca
     4621 cgagcaggcc gctgccgccg ccctggctgc cgtcggggtg ggagaggccg ggcagggcga
     4681 cgatcggcgt cccgaacaaa ttgaggctgg cgccctggta gcggatccgg ttctcgaccg
     4741 gatcgtggac cacttggacg gcggtgatgg tccaggtcgg atctttgggg cagccattct
     4801 cgtcgaccac ggcgcaaggc gagtaagcgg cgcggaacag ggtcgtatag ccttcccgcc
     4861 gctcgccccg gtcggcggcg agccggccgc catcctcgag caccagcagc agattctgga
     4921 ccatgccgtc gcggagcgtg tcggtgagga cgatgctgtc gccataagcg gcgtcgccct
     4981 gcgggttgac gatgcggaca ttgccttcgg cgcgcacttc gccgctctgc cggttccaga
     5041 tcacccggtc ggcgcgcaga ttattgcctt cgctggtcat gcgcacgttg ccggtcgccg
     5101 tcacgacctc gacgcttgtg tcgtagacga gctcgtcgga gctgaaatcg acctgccgct
     5161 ccgccgccgg agcggccgga tcggcggtct cctgcgcatg gaggatgccg ggcgcgcaaa
     5221 gcagcagggg caaagccgtg cggcagagga agttgagcgt cttcactgtc gttcgctgac
     5281 ttttgccccc gccgcattgg cactcgcgac gatggagcct atcgcaccgg aactcgttcc
     5341 ctgcaatggc ggaagctggc ccgtgccctt tgccagcgac ctcgctcttg cctatcaggc
     5401 gtccacccga aatttccgac caggaggtct cttcattcca tgaaagtcag tttcgccacc
     5461 cagcgcccga gcggcgccta tgcgctcgct atccccgtct ggagcgaaga cacgctggcc
     5521 gcgcggctcg ccggcctcga tgacggcgcc cgcacgctcg cgacccgcgc cgccgacgcc
     5581 cagcgtttcg agcgcgaggc ggcggttatc gccgaaacct tcgtcaccga gggcgacatg
     5641 gcgcggcggc tgctgctggt cggcctcggc ggccgccgcg acgacgacgc gatctatgag
     5701 aaagccggcg gcgcgctgac cgccaagctg ctgacctcgg gcgagacccg gctggtgatc
     5761 gatctcgacg gcctcggcct cgacggcgag gcggcggcgc ggatcgcgtt cggcgcggca
     5821 gcccgcggct ggcgctacga catctaccgc accaagctcg gcaagaagag caagccgacg
     5881 ctggaggaga tcgtgatcgt cggcgccgag ggcgacgccg acggcgagtg gcagcgcaag
     5941 gccgccttgt tcgaagggct tgcgctgacc cgcgaactcg tcaccgagcc cgccaacatc
     6001 atctaccccg agaccttcgt cgagcgctgc cgcgccgcgc tggacggcac gggcgtcgag
     6061 atcgaagtca tggacgaaaa ccggatgcgc gagctcggca tgggcgcgct gctcggcgtc
     6121 gcccagggtt cggtgcggcc gccgcggctg ctggcgatgc gctggaacgg cgccgccgac
     6181 gccgccgcac ggccggtcgt gctggtcggc aagggcatca ccttcgacac cggcggcatc
     6241 tcgataaagc cgtcgctggg catggaagcg atgaagtggg acatgggcgg cgccggtgcc
     6301 gtggccggca cgatgaaggc gctcgccacc cgcaaggcgc gcgcctacgt ggtcggcatc
     6361 tgcgtgctcg ccgagaacat gcccgacggc aacgcgcagc ggccgggcga cgtcgtgacc
     6421 tcgatgtccg gccagaccat cgaggtgatc aacaccgacg ccgagggccg gctggtcctc
     6481 gccgacgcga tgacctgggc gcagcgcgag cataagcccg aagtgatgat cgacctggcc
     6541 acgctgaccg gcgcgatgat catctcgctc ggccacgaat atgccggcct gttcagcaac
     6601 gacgacggtc tcgccggcaa gctcgtcgcg gcgggccagg cgtccggcga caagctgtgg
     6661 cggatgccgc tcggcgacgt ctacgacaag ctcatggact cggcgatcgc cgacatgaag
     6721 aatagcggcc cgcgcgaggg cggctcgatc accgccgcca ccttcctgca gcgcttcgtc
     6781 gacgaagggg tgaagtgggc gcatctcgac attgccggca tggtctgggc gaacaaggcc
     6841 ggccatctct acgacaaggg cgcgaccggc tacggcgtcg ccctgctcga ccggttcgtg
     6901 gccgacaatt acgaggggtg aggctggtaa gaggggtgag gctggccgcc gcgctcccgg
     6961 caagggcgta aggtgatgct ggtcgatttc taccatctcg ccacgacgcc gctcgagcgg
     7021 gtgctgccga gcatctgcga gaaggtgctg gccggcggcg agcggctgct ggtggtcgcc
     7081 gaagctgacc tgatcggcca gctcgacacg ctcctgtgga gctatgcgcc gggctcgttc
     7141 ctcccgcacg ggcgggcggg cggtccggcg ccgggcgagc agccggtgct gctttcggaa
     7201 gaccccgagc cgctcaacgg cgcgaccaac atcgcgctcg ccgacggccg ctggcgcgac
     7261 gaggcgctcg ccttcgcccg cgccttctat ttcttcgatg ccggccagct cgacgccgcg
     7321 cgcgcgagct ggcgcgcgct caaggacaag cccgaggtgg agcgccgcta ctggaagcag
     7381 gacggcggca aatgggtgca ggggccctaa agcgtcagcg gcgctgcata gccggtgagc
     7441 tcgagatagc cgcggccgcc ggtcgtggtg acggcgcctt cccaataagc cggcatgccg
     7501 ccgccgcgcc catcgagctc ctgcgcgtcg aacaagggcc tcagcgcaaa gcgccgctcc
     7561 ccctcgggca gccggacgac gaagtccgcc tcgaccgggt aagtggcgcc ggtcgcgggc
     7621 gagcgccagc gccggcgcgg cacgaagcgc acttcgccag gccccagcgc aagccggctg
     7681 ccgttgggcc gacgcaacgt cccgcccgca taaagggcgc gcccgtcctt gccgcgcacc
     7741 tggaacgcca tcagcgcgcc gccgccgtcg aggttgatcc ccgcccaatc ccacccgact
     7801 gcttcgggcg gcagcggcgc cgaggaccat tcgcggtcga gccaggcgct ccctcgcacc
     7861 ggcacccgcc ggccgccgcg cgtcaccgac cccgacgcgg cgagatgcgg catcgaataa
     7921 taatagctag cctggctggg cgccggaccc ttgcggctgt agccgccggc gccgttaggc
     7981 agcgtccgct gggtgggccg cagcaccagg tccagcgtga agcccttggc ggggatccgt
     8041 gcctggaacg cgccgtcggg cctgcgcacc agcgaccaat cgagcagccg caccgccgca
     8101 tcgccggtgc cggcgtccgc gagaccgaag ccggcccgcg ccgcccgctg gtcgtggagc
     8161 agccgccggc ttgtgggatc ggacagagcg gcgtgcgcga acacgatctg gcgcggcgcg
     8221 aacgcgcttg ggttggcggg atccacggcg gggcgcgagc ggaagaaggt gatctggaac
     8281 ccgagtgggc ccgcgtccgt ctcgagccag ccggtgaaat accaccattc ggtacggtaa
     8341 tcgggatggg cgccatgatc gcgcgggaag cggagctcgg cacccggctt cacttccgga
     8401 taggcgatcc ccggccgccc cgccggctgc gccgccagcg ccgcgccggc cgccagcagg
     8461 gccagtgccc aggcccgcac ccgcatcacc aatcctccct aaccgcgcgg acggcgtcgg
     8521 tgctgaccgc gcgccggccc gccagtaccg ccgtcccggc cgaagtgagg acgagcgccc
     8581 ccgcgacccc cagcagcagc ccccagggca ggcgcgtctc catcgtccag ttgaacgatt
     8641 gcgggttgat gacgtggatc aggatctggc tgatcgcgag gccgagcgcg gttccggcgg
     8701 cgatcccgat cagccccagc agcgcgccct cgccggccag catcgcgacg atcgcgcgcg
     8761 ggctcatgcc gatatggcgg agcatgccga attcgcggat gcgggcgatg gtctgcgcgg
     8821 acatggtcgc cgcgactccg gccagcccga tcaggatggc gatggcctcg agcacatagg
     8881 tgatggcgaa gctgcggtcg aacaggctga gcgcaagtct ccgcagcgtc tccggctccg
     8941 ccagttgcac gcggccggcg atgcccggcg ggagccgcct tcggatcgct tcgccgacct
     9001 tggccgccga ggctcccggc gcgagctcga ccgcggcctc gctccgcagc cggtcgccgg
     9061 tcagcgcggc atagtcgccc gaatcgatca cgatcgcgcc ttgctgccgc acataatcgc
     9121 tccacacccc ggcgacatag ccgtccgccc cgccgccgat cggcaggcgc acgctgtcgc
     9181 ccgcttcgac gccgtagagc ctcgccgccg gctccgaata ccagaaggcg gtgccgcggc
     9241 cccgggccgg cgcctcgcca atcagcggca gcgaatagcc cggcccgccg accggccgca
     9301 cgatcagctc gaccgagggg agggcggggt cgagcgtcag ccgcaccgcc ttgctgaagg
     9361 cgatgctggc gacccccgga gcgcccgcga gccgcgcctg caattcgggg tcgaacaaat
     9421 cctgctcgcc cccggccgcg acatagaggt cggcgctgag gaagctgtcc agccattcgt
     9481 cgaccgagcc gcggaagctg gtcaccatca ccgccatcgc gatcatcagc ccggtcgagg
     9541 cgacgatccc gcccagcgcc accgccgcct gcgacggcgc gcccagcagc cgccgcagcg
     9601 cgagctcgaa cggcggcagc ttcgaatcga accgggccag cggccgcagc aggtggcgcg
     9661 ccagccacgg catcgccgcc acgccgccgg ccagcaccag cccgaccgcg gcatagccga
     9721 acacaggcag ccggccgacc gcgggcagga aagccgcgcc cgcgcccatc agcagcagga
     9781 tcgccgccgg aatccaggcc ggtcgcctgc gcgggtcgac gatgtcgccg gcattcttga
     9841 gcgcgtcggc gggggcgatc cgcgccgcct gccgcgccgg cgcgtagctg ccgccgatgg
     9901 ccgtcaggat gccgaagccg aggaacagga aagcggccgc gggcgcgaaa tggaggtcgg
     9961 gcgacgcgcc gccgaaatag ccgccgccaa ggtcgccgcc gacgaatcgc atcgccagcg
    10021 cggcgaggcc gtagccgagc gcgagccccg ccagcgatcc ggcgatgccg agcaacgcgc
    10081 cttcgccgat cagctgcgcg cgcagcgccc gttcctgcag ccccagcgtg cgcagcaagg
    10141 caaactggcg cagccgccgc gcgaccgaca aggattgcgc cgaatagacc aggaagccgc
    10201 cggtcagcag cgcgaccaaa gccagcatgt cgagattgac gcggtaggcg cgcgacaggc
    10261 tgtcgccgcg gcggctttca ttctcggcat cgctcagcac cgcgcccgcc ggcagggcgg
    10321 cggcgatctc ccgccgcgcc gccgcttcgc cgaggccatc ggccagcttg aggtcgaccc
    10381 gatcgagcct gccgagcctc ccgaaccgcc attgcgccgc cgcgatgtcg accatggcga
    10441 ctacctggcc ggcgggcacc ccgggcaggt cgccggcgac gatgaagacg tggctgttcg
    10501 ggccgacctg caccacagcg ggctgccccg gccgcagtcc cgtccgcgcc tgcgccgtct
    10561 ccgacaaata gagcgcggtc atgtcgaagg cggcgtccga cattgccggg cggctgctcg
    10621 tcccgccgtc cgtccccgtg agccgccgcc cgagcaggtt gggcgtgacc gcgagcgccc
    10681 tcagcacgtc gatgccgagc agcgcgacgc gctccttgcc gatggtggcg ccgagctgca
    10741 ccaccgggct ggcatcggcc accgccgcca gctcgaacaa tttcgggtag agtctctcgt
    10801 cgaagccggt gccgccggcg gcgcggacct gcaaatcggc gtcccccgac acgctccgca
    10861 ccgcctcacc gaaggcgtcg gcggccgagc ggttgatgac gtggaccgta aaggcgagcg
    10921 cgacgccgac cgcgatggcg aggccggcca gcaggaagcg cagcggaaac gcccgccact
    10981 cgccggcgat cagccagcgc cacgcgccgc ccgcggccgc gcgctcagcc atccgacgcc
    11041 accagcccgt gccgcgtcag cagcagccgc cggtccgcgc gtcccgccgc agccgggctg
    11101 tgggtgacga gcagcatcgc cgccccacgc tgcttcaccg ccccggtcag caggttcagg
    11161 atctggtcgg cggtctcggg atcgagattg ccggtcggct cgtcggcgag aatcaaagcg
    11221 ggccggtgca ccagagcgcg ggctatggcg acgcgctgga gctcgccgcc cgacaattcc
    11281 ttgggatagc tgcgcagccg gtcgccgagg ccgaccgcgc tgagcaacgc cgccgcctcg
    11341 tcgagcgccg ccgcccgcgg ctgccgctgc aggaccagcg gcagcgcgac attctgcgcc
    11401 agtgtcagat gggggaggat atggaaggcc tggaacacga agccgatcc
//
LOCUS       DASVRK010000226         8872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_107396, whole genome shotgun sequence.
ACCESSION   DASVRK010000226 DASVRK010000000
VERSION     DASVRK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107396"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1248
                     /gene="murG"
                     /locus_tag="VF589_09015"
     CDS             13..1248
                     /gene="murG"
                     /locus_tag="VF589_09015"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX8257763.1"
                     /translation="MMPSRHYVLAAGGTGGHMVPAHALAEELMLRGHRCALITDDRGA
                     RIPGLFDKVQVHILPAGRLGGGPFGWIRAARNILTGRAMASRLYETFRPSAVIGFGGY
                     PALPALLAAQKNGVPTLIHEQNAVLGRVNRFVARRVDAIATAYPEVDRLSPKHHAKTH
                     LVGNPVRDEVLAIREQPFPPLTENGVFRVLVTGGSQGASILSDVVPEGLALLPESLRR
                     RLQVTQQCRAEDIEQVRARYANHGIPADLATYLTDMPERLAWSHLVIARAGASTIAEL
                     TAAGRPAILIPLPTATDDHQTANAREMAKSGGARVMRQTKLSAAQIADAEIVPAGHER
                     KHTAGRFTPLELSRQMQKLALQPGALANAAERAKAVGRPHAARDLADLVERTGRDPAA
                     DALVADEMVLRTMPQGAYA"
     gene            1327..2739
                     /gene="murC"
                     /locus_tag="VF589_09020"
     CDS             1327..2739
                     /gene="murC"
                     /locus_tag="VF589_09020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX8257764.1"
                     /translation="MRGVGTDIGTIHFIGIGGIGMSGIAEVMHILGYKVQGSDVAEGY
                     VVEGLRKAGIPISIGHDPDNLGDAAVVVTSTAIRKDNPEVVCAYERRIPVVRRAEMLA
                     ELMRLKATVAVAGTHGKTTTTSMVAALLDAGGIDPTVINGGIINAYGSNARLGKSDWM
                     VVEADESDGSFLRLDGTIAVVTNIDPEHLDHYGSFEKVKDAFVEFVENVPFYGAALLC
                     VDHPEVQTIIPRLRDRRVVTYGFAAQADVRGDNVVPFPGGNRFDVTIRSRTGELRTLI
                     GVELPMPGRHNVQNALAAIGVAAELGIADEVIVRGFEKFDGVKRRFTKVGEIDGATII
                     DDYGHHPVEIKAVLAAAREGAKGRVIAAVQPHRYSRLRDLMDEFQGAFNDADIVYVAP
                     VYPAGEEPIEGVDNHALVEGLKMRGHRAARTVDGPEDLCRQLRDIAARGDMVICLGAG
                     DITKWAAGLADGIRQSRLNK"
     gene            2736..3740
                     /gene="murB"
                     /locus_tag="VF589_09025"
     CDS             2736..3740
                     /gene="murB"
                     /locus_tag="VF589_09025"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX8257765.1"
                     /translation="MSVPEAKHPFVSSEVETPSRAKPREEAQPRRADIPALAGSAEPG
                     GSLADFIWFRTGGPAEWLVRPKDVVDLSRFLAALDPAVPVLPVGVGSNLIVRDGGVPG
                     VVVRLPKSFAKVEAEPGHRVRAGGGAMGITVASKARDAGIAGLEFLRGIPGTAGGAVR
                     MNAGAYGREVRDILIEATVVLRDGSVETWPAERFGYTYRHSELSAGAVVVEALFEGVP
                     GDAKAIGAEMDRIAEEREASQPLRSRTGGSTFKNPRGIKAWQAIDAAGCRGLKIGCAQ
                     VSEKHCNFLLNLGNASAADIEALGEEVRRRVLEHSGVALEWEIQRVGLGRRGAGEEGV
                     "
     gene            3737..4675
                     /locus_tag="VF589_09030"
     CDS             3737..4675
                     /locus_tag="VF589_09030"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX8257766.1"
                     /translation="MTRAKLHVAVLMGGWSAEREVSLSSGTGVADALESLGHQVSRID
                     MDRNVARVLEEIRPDVVFNALHGTPGEDGTIQGLLDLMGIQYTHSGLVTSVIAIDKEL
                     TKQQLVPHGIRMPGGEIVASASLHERDPMARPYVLKPVNEGSSVGVAIVTHAGNYGNP
                     IGRDVEGPWQQFDRLLAEPFIRGHELTVAVLGDEALAVTELKPTQGFYDYDAKYTDGL
                     TQHVCPAEIPDEIAEAAMAIALRAHRALGCKGTSRSDFRWDDERGLDGLYLLEVNTQP
                     GMTPLSLVPEQAKYRGMSYAELVQRIVDDALAGERP"
     gene            4672..5640
                     /locus_tag="VF589_09035"
     CDS             4672..5640
                     /locus_tag="VF589_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX8257767.1"
                     /translation="MTARIARGSSAAKPRSKPVARGRSGSGSGSGSKKQQQQQPGMLD
                     SLPVSAEAIRRVSLWILFAMLLALAIAVAAALRVPQMIGNSIGESVGQAGFVVRRVEP
                     KGLDRMNPMQVYLIAGDQLDRAMPLVDLDGTRARLLRFGWVHDARVSRRWPDTLVVDI
                     VERKPAAIWQHNQRLILIDPDGVVLEEVKLEAMPDLPLVIGPAANRHAGELNQLLSVS
                     PEIKPMMAGATWIGGRRWDLRFQSGEVLALPEGLAAARKALSTFAKMDKTSQLLGRGF
                     ARFDMRIPGKFIVRLSNVPGTTVPVLEPETPPAAAGSGPIDPATTI"
     gene            5637..6905
                     /gene="ftsA"
                     /locus_tag="VF589_09040"
     CDS             5637..6905
                     /gene="ftsA"
                     /locus_tag="VF589_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX8257768.1"
                     /translation="MRPKAPPPARKLVTALDIGSSKVSALIAEPAEDGSLKILGTGQR
                     ESRGVKRGFVADMEKSEAAIREAVEQAERIAGTNIDEVFVGWSAGGLVSTVASVEVDI
                     GGRRIEQHDIDDLLGAGKSSINPEGRMLLHALPALYTLDGLTGVKQPMGLHAGKLAVD
                     IHVISAEPSPVRNLDLCVRSAHLGVTAIVASPVAAGKACLSEEERELGVALVELGAGV
                     TNVSVFAGGMLVGLKSIAMGGIDITDDIASAFGTRRLEAERMKCFYGSATTSPRDNHD
                     MIELVPIAGAEEGTESARITRAQLIAVIRQRLEHNMGEIATALKELGFSGPFGRQVVL
                     TGGGSELKGMADYAQGVLGRAVRIGRPKMPGLPEAHSGPAFATLAGLAQFAASDELDL
                     RQLSAAETNVQKPEGGNVVQRLIAAFRTQY"
     gene            7013..8479
                     /gene="ftsZ"
                     /locus_tag="VF589_09045"
     CDS             7013..8479
                     /gene="ftsZ"
                     /locus_tag="VF589_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8257769.1"
                     /translation="MSIDFIRPEVDELRPRISVIGVGGAGGNAVANMINSDVQGVDFI
                     VANTDAQALNASAADRRIQLGLKITQGLGAGSRPEIGKAAAEETIAEIEKALEGAHMC
                     FIAAGMGGGTGTGAAPVIAKAARDRGILTVGVVTKPFSFEGSRRGRSADAGIDELQQH
                     VDTLIVIPNQNLFRIANPNTTFKEAFAMADEVLQQGVRGITDLMVMPGLINLDFADVR
                     SVMGEMGKAMMGTGEASGDNRAIEAAEKAIANPLLDGVSMKGAKGVIISITGGEDMRL
                     MEVDEAASHIKELVDPDANIIWGSAFNNDLEGKIRVSVVATGIDAEANVQAAPAKVFA
                     FPNARGVEAVKPAPRPVPVPEPQIIAEAAPQATPAAIELNLQEEEIEVAEQPMAATPM
                     EEDSDELVLGSGDMLAGETEEEEADAGEPARGTRAWLAQQETAPPAAARAAGGTLFER
                     MSNIARGAAKAQVEEEAPARSARDPLDIPRFLNRQNNQ"
     gene            8605..>8872
                     /locus_tag="VF589_09050"
     CDS             8605..>8872
                     /locus_tag="VF589_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8257770.1"
                     /translation="MTKSKRAGLALLAAGLLSGTGLTAPAAAQYAAQPYRPAPVPYRP
                     APAPAPYREDAGTALTRHLRTLADQPRSLHALSGAGRAALELGDP"
BASE COUNT         1484 a         3088 c         3022 g         1278 t
ORIGIN      
        1 cggagagcgg cgatgatgcc ttcgcgccac tatgtcctcg ccgccggcgg caccggcggc
       61 catatggtgc cggcccatgc gctggccgag gaactgatgc tgcgcggcca tcgctgcgcg
      121 ctcatcaccg acgatcgcgg cgcccgcatt ccgggcctgt tcgacaaggt tcaggtccac
      181 atccttcccg ccggccggct cggcggcggc ccgttcggct ggatccgggc ggcgcgcaac
      241 atcctcaccg gccgcgccat ggcctcgcgc ctctacgaga ccttccgccc ctcggcggtg
      301 atcggcttcg gcggctatcc ggcgctgccg gcgctgctgg cggcgcagaa gaacggcgtg
      361 ccgaccctga tccacgagca gaatgcggtg ctcggccggg tcaaccgctt cgtcgcgcgc
      421 cgcgtcgacg ccatcgccac cgcttatccc gaggtcgacc ggctgtcgcc caagcaccac
      481 gccaagaccc atttggtcgg caaccccgtc cgcgacgagg tcctcgccat acgcgagcag
      541 ccgtttccgc cgctgaccga gaatggcgtg ttccgcgtgc tggtgacggg cggcagccaa
      601 ggcgcctcga tcctgtccga cgtcgtgccc gaggggctgg ccctgctccc ggagagcctg
      661 cgccgccggc tccaggtcac ccagcaatgc cgggccgagg atatcgagca ggtgcgggcg
      721 cgctatgcca atcacggcat cccggccgac cttgccacct atctcaccga catgccggaa
      781 cggctcgcct ggtcgcacct cgtcatcgcg cgcgccggcg cctcgaccat cgccgagctg
      841 accgccgccg gccggccggc catcctgatc ccgctgccga cggcgaccga cgaccaccag
      901 accgcgaacg cgcgcgagat ggccaaatcg ggcggcgcgc gggtgatgcg ccaaaccaaa
      961 ttgagcgccg cccagatcgc cgacgccgag atcgtgccgg ccgggcacga gcgcaaacac
     1021 accgccgggc ggttcacgcc gctcgagctt tcgcgccaga tgcagaagct cgcgctccag
     1081 cccggcgccc tcgccaatgc cgccgagcgc gccaaggcgg tcggccggcc ccatgccgcg
     1141 cgcgacctcg ccgacctggt cgagcggacc gggcgcgacc ccgccgccga cgcgctcgtc
     1201 gccgacgaga tggtgctccg tacgatgccg caaggcgcct acgcatgacg gggtgccgtg
     1261 catgaggggt gcccttcgac cggctcagga tgagcggctg ccgacacccc ccatcggaag
     1321 cctccgatga ggggtgtcgg caccgacatc ggcaccatcc acttcatcgg cattggcggc
     1381 atcggcatgt cgggcatcgc cgaagtgatg cacatcctcg gctataaggt gcagggctcg
     1441 gacgtcgccg aaggctatgt cgtcgagggg ctgcgcaagg cgggcatccc gatcagcatc
     1501 ggccacgatc ccgacaatct cggcgacgcg gcggttgtgg tgacctcgac cgcgatccgc
     1561 aaggacaacc cggaagtcgt ctgcgcctat gagcggcgca tcccggtcgt ccgccgcgcc
     1621 gagatgctgg ccgagctgat gcggctgaaa gccaccgtcg cggtcgccgg cacccacggc
     1681 aagaccacca ccacctcgat ggtcgcggcc ctgctcgacg ccggaggcat cgatccgacc
     1741 gtgatcaacg gcggcatcat caacgcttat ggctccaacg cacggctcgg caagagcgac
     1801 tggatggtgg tcgaggccga cgagagcgac ggcagcttcc tgcggctcga cggcacgatc
     1861 gcggtcgtca ccaacatcga tcccgagcat ctcgaccatt atggatcgtt cgagaaggtc
     1921 aaggatgcgt tcgtcgagtt cgtcgagaac gtgcctttct acggcgccgc gctgctgtgc
     1981 gtcgatcatc ccgaggtgca gacgatcatc ccgcggctgc gcgaccggcg ggtggtgacc
     2041 tacggcttcg ccgcccaggc cgacgtgcgc ggcgacaatg tcgtgccgtt ccccggcggc
     2101 aatcggttcg acgtcacgat ccgcagccgc accggcgagc ttcggaccct gatcggcgtc
     2161 gagctgccga tgcccgggcg gcacaatgtc cagaatgcgc tggcggcgat cggggtcgcg
     2221 gccgagctcg gcatcgccga cgaggtgatc gtgcgcggct tcgagaagtt cgacggcgtc
     2281 aagcgccgtt tcaccaaggt cggcgagatc gacggcgcga ccatcatcga cgattacgga
     2341 caccatccgg tcgagatcaa agcggtgctc gcggccgcgc gcgagggcgc gaaggggcgg
     2401 gtgatcgccg ccgtccagcc gcaccgctac tcgcgcctcc gcgacctgat ggacgagttc
     2461 cagggcgcct tcaacgacgc cgacatcgtc tatgtcgcgc cggtctatcc ggccggcgag
     2521 gagccgatcg agggcgtcga caaccatgcg ctggtcgagg ggctgaagat gcgcggccac
     2581 cgcgccgccc gcaccgtcga tggccccgag gacttgtgcc gccagctgcg cgacattgcg
     2641 gcgcgcggcg acatggtgat ctgcctcggg gcgggcgaca tcacgaagtg ggcggcgggc
     2701 ctcgccgacg gcatccgcca gtcgaggctc aacaaatgag tgtcccggag gccaagcatc
     2761 cgttcgtgtc gagcgaagtc gagacgccct cccgagcaaa gccgagggag gaagcgcagc
     2821 caaggcgagc ggacattcca gcgcttgccg gatcggccga gcccggcggc agcctcgccg
     2881 acttcatatg gtttcgcacc ggcggcccgg ccgaatggct ggtgcggccg aaggacgtcg
     2941 tcgacctgtc gcgcttcctc gccgcgctcg atcccgccgt cccggtgctg ccggtgggcg
     3001 tcggatcgaa cctcatagtc cgcgacggcg gggttcccgg cgtagtggtt cggctcccga
     3061 aaagcttcgc caaagtggaa gccgagccgg gccaccgggt gcgcgcgggc ggcggggcga
     3121 tggggatcac cgtcgcctcc aaggcgcgcg acgccggcat cgccggcctc gaattcctgc
     3181 gcggcattcc cggcaccgcc ggcggagccg tgcggatgaa cgccggcgcc tatggccgcg
     3241 aagtgcgcga catattgatc gaagccacgg tcgtgctccg cgacggcagt gtcgaaacct
     3301 ggccggccga acgcttcggc tacacctacc ggcatagcga gctttcggcc ggggcggtgg
     3361 tggtggaagc cttgttcgaa ggggtcccgg gagacgccaa ggcgatcggc gccgagatgg
     3421 acaggatcgc cgaagagcgc gaagcgagcc agccgctgcg gagccgaacc ggcgggtcga
     3481 ccttcaagaa tccgcgcggc atcaaggcct ggcaggcgat cgacgccgcc ggctgccgcg
     3541 gcctcaaaat tggctgtgca caagtctctg aaaagcattg caacttcctc ctgaatttgg
     3601 gcaatgctag cgccgcggat atcgaggcgc tcggcgagga agtgcggcgg cgggtgctcg
     3661 aacattcggg cgtcgcgctg gaatgggaaa tccagcgcgt tgggctcggt cgccggggag
     3721 cgggggaaga gggggtatga cgagggccaa gcttcacgtc gcggtgctga tgggcggctg
     3781 gtcggccgag cgcgaagtct cgctgtcgag cggaaccggg gtcgccgacg cgctcgaaag
     3841 cctcggccac caggtcagcc gcatcgacat ggaccgcaac gtcgcccggg tgctggagga
     3901 gatccggccc gatgtggtgt tcaacgcgct ccacggcacg cccggcgagg acggcaccat
     3961 ccagggcctg ctcgacctga tgggcatcca atatacccat agcggcctcg tcacctcggt
     4021 gatcgcgatc gacaaggagc tgaccaagca gcagctcgtg ccgcacggca tccgcatgcc
     4081 cggcggcgag atcgtcgcca gcgcgagcct ccacgagcgc gacccgatgg cgcggcctta
     4141 tgtgctgaag ccggtcaacg aaggctcgtc ggtgggcgtg gcgatcgtca cccacgcggg
     4201 caattacggc aacccgatcg ggcgcgacgt ggaagggccg tggcagcaat tcgaccgcct
     4261 gctcgccgag ccgttcatcc gcggccacga gctcaccgtc gcggtgctcg gcgacgaggc
     4321 gctggccgtc accgagctca agccgaccca gggcttttac gattacgacg ccaaatatac
     4381 ggacggcctc acccagcatg tctgcccggc ggaaataccg gacgagatcg ccgaggcggc
     4441 gatggcgata gcgctgaggg cgcaccgcgc gctcggctgc aagggcacgt cgcggtccga
     4501 tttccgctgg gacgacgagc gcggcctcga cggcctttac ctgctcgagg tcaacaccca
     4561 gcccggcatg accccgctga gcctggtgcc ggagcaggcg aaatatcgcg gcatgagcta
     4621 tgccgagctg gtccagcgga tcgtcgacga cgcgctcgcg ggagagcggc catgaccgcc
     4681 cgcatcgccc gtggctcgtc cgccgccaag ccgcgctcga agccggtcgc ccgcggccgg
     4741 agcgggagcg ggagcgggag cgggagcaag aagcagcagc aacagcagcc cggcatgctc
     4801 gattccctgc cggtctcggc cgaggcgatc cggcgcgtca gcctgtggat cctgttcgcc
     4861 atgctgctcg cgctcgccat tgcggtcgcg gcggcgcttc gggtgccgca gatgatcggc
     4921 aattcgatcg gcgaaagcgt cggccaggcc ggcttcgtcg tccggcgggt ggagccgaag
     4981 gggctcgacc ggatgaaccc gatgcaggtc tatctgatcg ccggcgacca gcttgatcgg
     5041 gcgatgcctt tggtcgatct ggacggcacg cgcgcccgcc tgctgcgttt cggctgggtc
     5101 catgacgcgc gtgtgtcgcg gcgctggccg gatacgctgg tggtcgacat cgtcgagcgc
     5161 aagccggccg cgatctggca gcacaatcag cggctgatcc tgatcgatcc cgacggagtg
     5221 gtgctcgagg aggtgaagct cgaggcgatg cccgatctgc cgctggtgat cggccccgcc
     5281 gccaaccgcc atgccggcga gctcaaccag ctgctctccg tcagccccga gatcaagccg
     5341 atgatggccg gcgcgacctg gatcggcggc cggcgctggg acctgcgctt ccagtccggc
     5401 gaagtgctgg cgctccccga aggtctggct gcggcgcgca aggcgctgtc gaccttcgcg
     5461 aagatggaca agaccagcca attgctcggc cgcggtttcg cgcgtttcga catgcggatc
     5521 cccggcaagt tcatcgtccg cctgtcgaac gtccccggca ccacggtgcc ggtgctcgaa
     5581 ccggaaacgc cgcccgccgc cgccggcagc ggcccgatcg atccggcgac cacgatttga
     5641 gacccaaggc cccccctccc gcgcgcaagc tcgtcaccgc gctcgacatc ggctcgtcga
     5701 aagtctcggc gctgattgcc gagccggccg aggacggctc gctcaagatc ctcggcaccg
     5761 gccagcgcga gagccgcggc gtcaagcgcg gcttcgtcgc cgacatggag aagagcgagg
     5821 ccgcgatccg cgaggcggtc gagcaggccg agcgcatcgc cggcaccaat atcgacgagg
     5881 tgttcgtcgg ctggtcggcg ggcggcctcg tcagcaccgt cgccagcgtc gaggtcgaca
     5941 tcggcggccg caggatcgag cagcacgata tcgacgacct gctcggcgcc ggcaagtcga
     6001 gcatcaatcc ggaaggccgg atgctgctcc atgcgctgcc cgcgctctac acgctggacg
     6061 gcctcaccgg ggtcaagcag ccgatggggc tgcatgccgg caagctcgcg gtcgacatcc
     6121 acgtcatttc ggccgagccg tcgccggtcc gcaacctcga tctgtgcgtg cgctcggccc
     6181 atctcggggt gaccgcgatc gtcgcttcgc cggtcgcggc cggcaaggcc tgcctcagcg
     6241 aagaggagcg cgagctcggc gtcgccctgg tcgagctcgg cgccggcgtc accaacgtct
     6301 ccgtcttcgc cggcggcatg ctggtcggcc tcaagtcgat cgcgatgggc ggcatcgaca
     6361 tcaccgacga catcgcgtcg gcgttcggca cgcggcggct cgaggccgag cggatgaaat
     6421 gcttttacgg atcggcgacg acaagcccgc gcgacaatca cgacatgatc gagctggtcc
     6481 cgatcgcggg cgcggaggag ggcacggaat ccgcccgcat cacccgcgcc caactgatcg
     6541 ccgtcattcg ccagcggctc gagcataaca tgggcgagat tgctaccgca ctgaaagagc
     6601 ttggtttttc aggcccgttc ggccgtcagg tggtgctgac cggcggcgga tccgaactca
     6661 agggcatggc cgattacgcg cagggcgtgc tcgggcgggc ggtgcgcatc ggccggccca
     6721 agatgccggg cctgccggag gcgcatagcg gcccggcttt cgccacgctt gccggcctcg
     6781 cccagttcgc cgcgtccgac gaactcgacc tgcggcagct ttccgccgca gaaacgaatg
     6841 tgcagaaacc ggagggcggc aatgtcgttc aacgactgat cgcggcattt aggactcagt
     6901 attaaggaaa attacaaggc tgttaacttt ttgctcgcgc ccaagaatcg catcgtgcaa
     6961 aagaatccga acggtgcttc gcgagcgccg ctgtaccagg ggagagaagc gtatgagtat
     7021 cgatttcatc cgtccggagg ttgacgaact tcgcccccgg atcagcgtca tcggcgtcgg
     7081 cggagccggc ggcaatgcgg tcgccaacat gatcaacagc gacgtccagg gggtcgactt
     7141 catcgtcgcc aacaccgacg cgcaggcgct caacgcttcg gcggcggacc ggcgcatcca
     7201 gctcggcctc aagatcaccc agggcctggg ggcaggctcg cggcccgaaa tcggcaaggc
     7261 cgccgcggaa gaaacgatcg ccgaaatcga aaaggcgctc gagggcgcgc acatgtgctt
     7321 catcgcggcc ggcatgggcg gcggcaccgg caccggcgcc gctcccgtca tcgccaaggc
     7381 ggcgcgcgac cgcggcatcc tgaccgtcgg cgtcgtcacc aagccgttca gcttcgaggg
     7441 cagccgccgc ggccgttcgg ccgacgccgg catcgacgag cttcagcagc atgtcgacac
     7501 cctgatcgtc atcccgaacc agaatctgtt ccgcatcgcc aacccgaaca cgaccttcaa
     7561 ggaagcgttc gccatggcgg acgaagtgct gcagcagggc gtgcgcggca tcaccgacct
     7621 gatggtcatg cccggcctca tcaacctcga tttcgccgac gtccgctcgg tcatgggcga
     7681 aatgggcaag gcgatgatgg gcaccggcga agcctcgggc gacaatcgcg ccatcgaggc
     7741 ggccgaaaag gcgatcgcca atcctctgct cgacggcgtc agcatgaagg gcgccaaggg
     7801 cgtcatcatc tcgatcaccg gcggcgagga catgcgcctg atggaagtcg acgaggccgc
     7861 cagccacatc aaggagctgg tcgatcccga cgccaacatc atctggggct cggcgttcaa
     7921 caacgacctc gaaggcaaga tccgggtgtc cgtggtcgcc accggcatcg acgccgaagc
     7981 caacgtccag gcggcgccgg ccaaggtgtt cgccttcccg aacgctcgcg gcgtcgaagc
     8041 cgtgaagccc gcgccccggc cggtgccggt gccggagccg cagatcatcg ccgaggccgc
     8101 gcctcaggcg actcccgccg cgatcgagct caacctccag gaggaggaga tcgaagtagc
     8161 cgagcagccg atggcggcca cgccgatgga ggaagacagt gacgaactcg tgctcggcag
     8221 cggcgacatg ctggccggcg agaccgagga agaagaagcc gatgccggcg agcccgcgcg
     8281 cggaacccgt gcctggctgg cgcagcagga aacggctccg cccgcggcag cccgcgcggc
     8341 cggcgggacc ttgttcgagc gcatgtcgaa catcgcccgc ggcgccgcca aggcgcaggt
     8401 cgaggaggag gctcctgccc gctccgcccg cgatccgctt gatatcccac gattccttaa
     8461 tcgccagaac aatcagtaac ttggcttaag ttccagtcgg tgccaccggt tcatcgccgg
     8521 tcggcaccga tttggcgcga acgtccgcgc cagccattgc cggtacagct tctctcaggc
     8581 ttatcggcgg gcatgacttc cgcaatgacg aagagcaaac gggctggcct ggcactgctg
     8641 gcggcgggcc tgctgtcggg caccgggctg accgcgcccg ccgccgcgca atatgcggcc
     8701 cagccttatc gtcccgcccc cgtgccctat cgcccggccc ccgcgccggc gccctatcgc
     8761 gaggatgccg gcaccgcgct cacgcggcat ctgcgcacgc tcgccgacca gccgcgcagc
     8821 ctccatgcct tgagcggcgc cggccgcgcc gcgctcgaac tgggcgaccc gc
//
LOCUS       DASVRK010000227         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_234049, whole genome shotgun sequence.
ACCESSION   DASVRK010000227 DASVRK010000000
VERSION     DASVRK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234049"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="VF589_09055"
     CDS             complement(<1..1795)
                     /locus_tag="VF589_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX8257771.1"
                     /translation="MIAEAGLAALWLAAALSLLQLAFGCLALSSCSSLLGEGEGGPST
                     ARRAVPLPEASSGRNSVFAAVRPLAAAQALLVALSFLALILLFLRTDLSVALVAANSH
                     SAKPWLYKFAGTWGNHEGSMLLWVAVLGLAGGAVALFERRLAERTLVATLAAQAAIGL
                     GFYAFLLFASNPFERLSPAPPQGQGLNPLLQDPGLAFHPPTLYFGYVGISVAFSFAVG
                     ALVARDVGAGFARAMRPWVLGAWIFLTLGITAGSYWAYYELGWGGWWFWDPVENASLM
                     PWLAATALLHSVTVLATRDSLRAWTLMLAVVAFSMSMVGTFLVRSGILTSVHSFAVDP
                     QRGAFILVLLTLYIGGALLLFGLRVGTVREGATFEPVSREGALVANNLLLSAILGVVL
                     VGTLYPLAVEAATGEKLSVGPPYFNSAAGPIALALIAVMAAGPLIRWRRDRAGDVVKR
                     IAAPLLITAAALAASLLLAPGIGLLPRLGLVLAAGTAAASLAPLWGRNLKRTPLFTWG
                     MVVAHLGIAVSLAGMASESAFTRETLVAAAPGETVAVGPYRVRFDSVEPVAGPNWTAI
                     EGRLTASRGGADFLLRPQARTFSSPPTETSEA"
     gene            complement(1792..2226)
                     /gene="ccmE"
                     /locus_tag="VF589_09060"
     CDS             complement(1792..2226)
                     /gene="ccmE"
                     /locus_tag="VF589_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX8257772.1"
                     /translation="MKAKNQRLTLLALAIAAVLAAVLLAMSALKDQAAYFYAPADVAR
                     KGLPLDRAVRIGGMVEGGSIRRSPDGVTIDFVVGDETEAKIRVRFTGIAPDLFKENSG
                     VVAEGRFEPGGRFVATEILAKHDENYMPPQLAGEKHKSETLQ"
     gene            complement(2223..2354)
                     /gene="ccmD"
                     /locus_tag="VF589_09065"
     CDS             complement(2223..2354)
                     /gene="ccmD"
                     /locus_tag="VF589_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX8257773.1"
                     /translation="MNPWPFVVASYALTLIGVGGLTAWSFAAMRRAEAEAEALRRDR"
     gene            complement(2351..>2596)
                     /locus_tag="VF589_09070"
     CDS             complement(2351..>2596)
                     /locus_tag="VF589_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEX8257774.1"
                     /translation="NLPIIHYSVLWWRTLHQGQSVGLTGSSIHGSMLWPLLASAFGFS
                     LLFGAIVLMRMRAMLASGKVEARLKRMAASPSPGAAS"
BASE COUNT          481 a          975 c          843 g          297 t
ORIGIN      
        1 ccgcctcgct ggtttcggtc ggcggcgagc tgaaggtgcg cgcctgcggc cgcagcagga
       61 aatcggcgcc gccgcgcgaa gcggtcagcc gcccctcgat cgcggtccag ttcggccccg
      121 ccaccggctc gaccgaatcg aaccgcactc gatacggccc gaccgccacc gtctcgccgg
      181 gcgccgcggc caccaaggtc tcgcgggtga aggcgctctc cgaagccatg ccggccaggc
      241 tgacggcgat gccgagatgg gcgaccacca tcccccaggt gaagagcggc gtccgcttga
      301 ggttgcggcc ccacagcggc gccaggctgg cggcggcggt gcccgcggcc agcacaaggc
      361 cgagccgcgg cagcagcccg atgccgggcg cgagcagcaa cgacgccgcc agcgcggccg
      421 ccgtgatcag caacggcgcc gcgatccgct tcacgacgtc gccggcccgg tcgcgccgcc
      481 agcggatcag cggcccggcc gccatcaccg cgatcagcgc cagcgcgatc ggcccggccg
      541 cgctgttgaa ataaggcggc ccgaccgaaa gcttctcccc cgtcgccgct tccaccgcga
      601 gcggatagag cgtgccgacc agcaccacgc cgaggatcgc cgacagcagc agattgttcg
      661 ccaccaaggc gccctcgcgg ctgaccggct cgaacgtcgc gccctcgcgc accgtcccga
      721 ccctcagccc gaacagcaag agcgccccgc cgatatagag ggtgagcagg accaggatga
      781 acgcgccccg ctgcgggtcg acggcgaagc tgtggacgct ggtgaggata ccggagcgca
      841 ccaggaacgt gccgaccatc gacatcgaga aagcgaccac cgccagcatc aatgtccacg
      901 cccgcaggct gtcgcgggtg gcgagcacgg tcaccgaatg gagcaaggcg gtggcggcga
      961 gccagggcat cagcgaggca ttctcgaccg ggtcccagaa ccaccagccg ccccagccga
     1021 gctcataata ggcccaatag ctgccggcgg tgatgcccag cgtcaggaag atccacgcgc
     1081 cgagtaccca cggccgcatc gcccgcgcga agcccgcgcc gacgtcgcgc gccaccaaag
     1141 cccccaccgc gaaggagaag gcgaccgaga tgccgacata gccgaaataa agcgtcggcg
     1201 gatggaaggc gaggccggga tcctgcagca aggggttcag cccctgcccc tgcggaggcg
     1261 ccggactgag gcgctcgaac ggattggagg cgaacagcag gaaggcgtag aagccgagcc
     1321 cgatcgccgc ctgcgccgcc agtgtcgcga ccagggtccg ctcggcgagc ctgcgctcga
     1381 acaaggcgac cgcgccgccg gcaaggccga gcaccgccac ccagagcagc attgagccct
     1441 catgattgcc ccaggtcccg gcgaatttgt aaagccacgg cttggccgaa tggctgttgg
     1501 ccgcgaccag cgccaccgac aaatcggtgc gcaggaacag caggatcagc gcgagaaacg
     1561 acaaagccac cagcaacgcc tgcgccgccg ccagcggccg caccgcggcg aaaacagaat
     1621 tcctccccga gctcgcctcg gggaggggga ccgcgcgacg cgcggtggag gggccccctt
     1681 caccctcccc gagcaagctc gagcaggaac tgagcgcgag acatccgaac gccagctgca
     1741 gcagcgacaa ggcggccgcc agccacaaag cggcaaggcc cgcctcggcg atcattgcag
     1801 cgtctcgctc ttatgcttct cgccggccaa ttgcggcggc atgtaattct cgtcatgctt
     1861 ggcgaggatc tcggtcgcga cgaagcgccc gccgggctcg aaccgccctt ccgccaccac
     1921 gccgctattc tccttgaaca ggtccggcgc gatgccggtg aagcggaccc ggatcttggc
     1981 ctcggtctcg tcgccgacca cgaagtcgat ggtcaccccg tccgggctgc ggcggatcga
     2041 tccgccctcc accatgccgc cgatccgcac cgcccggtcg agcggcagcc ccttccgcgc
     2101 cacatcggcc ggcgcgtaga aataggcagc ctggtccttc agcgccgaca tcgccagcag
     2161 caccgccgcc agcaccgccg cgatcgccag ggccagcaat gtcagccgct ggttcttggc
     2221 cttcaccggt cccgcctcag cgcctcggcc tcggcctcgg cgcggcgcat cgcggcgaag
     2281 ctccacgcgg tcagcccgcc gacgccgatc agggtcagtg catagctcgc caccacgaac
     2341 ggccaggggt tcacgacgcc gctcccgggc ttggcgaagc ggccatccgc ttcagccgcg
     2401 cctcgacctt gccgctggcc agcatcgccc gcatccgcat cagcacgatc gctccgaaca
     2461 gcagcgaaaa gccgaacgcc gaggccagca gcggccacag catcgagcca tggatcgacg
     2521 atccggtaag gccgacgctc tgcccctggt ggagcgtgcg ccaccacaaa accgaataat
     2581 ggatgatcgg aaggtt
//
LOCUS       DASVRK010000228         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_543674, whole genome shotgun sequence.
ACCESSION   DASVRK010000228 DASVRK010000000
VERSION     DASVRK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543674"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /gene="tatB"
                     /locus_tag="VF589_09075"
     CDS             complement(<1..298)
                     /gene="tatB"
                     /locus_tag="VF589_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX8257775.1"
                     /translation="MFDIAPTELLIVALVALVVIGPKDLPRVLRTVGHWVGRARGMAR
                     HFRSGLDTMMREAELEEMEAKWRTENERIMRDFPSTGADPTPLPPAAAAQQGPAP"
     gene            complement(320..568)
                     /locus_tag="VF589_09080"
     CDS             complement(320..568)
                     /locus_tag="VF589_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8257776.1"
                     /translation="MGGLSLWHWLVVGVIILLLFGKGRFSEMMGDVAKGLKSFKKGMT
                     DEEEATPPPPRRIVEGDRTVDAAAERSRTADPEDRREP"
     gene            complement(677..1258)
                     /gene="scpB"
                     /locus_tag="VF589_09085"
     CDS             complement(677..1258)
                     /gene="scpB"
                     /locus_tag="VF589_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX8257777.1"
                     /translation="MTGGLGDDVRSVEATLFASDNPMTAAEIADYVGPDVDVSEALAI
                     LGEHYAGRGIELVERGKRWHFQTASDLAHILRRERDEPRKLSRAAVETLAIIAYHEPV
                     SRAEIEAIRGVQISKGTLDVLMEAGWVRPAGRREAPGRPLTYATTAGFLAHFGLAGRR
                     DLPGIDDLRAAGLLDPIEAAMDQLELESGRDED"
     gene            complement(1255..2016)
                     /locus_tag="VF589_09090"
     CDS             complement(1255..2016)
                     /locus_tag="VF589_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX8257778.1"
                     /translation="MDFDDDFDERPAVAAADRLTLDLDGWEGPLDLLLTLARTQKVDL
                     ARISILALTDQYLAFIHDAKSLKLEIAADYLVMAAWLAYLKSCLLLPRDAGADPSPEE
                     LAMRLQLRLQRLSAMREAGARLLGRDRLGRDVFARGAPEGLRTIRKAAWQVELYDLIA
                     AYGTIRARTEPAVHIVGRRPVMTLEEALLRVERLIGTSVRWVEIRSFLPETPDPEYRR
                     SALASSFVAALELARQGRLELEQESPFAPLYVRAA"
     gene            complement(2059..>2370)
                     /locus_tag="VF589_09095"
     CDS             complement(2059..>2370)
                     /locus_tag="VF589_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8257779.1"
                     /translation="VIADVIRGRIGYDGLLMSDDLGMHALSGGFGARARGVVEAGCDL
                     ALHCSGDMDEMVSVASALGEISEKGRERLNRAMATVADAGASADYAELAAKRDALLAY
                     A"
BASE COUNT          365 a          855 c          794 g          356 t
ORIGIN      
        1 cgggagcggg gccctgctgg gcggcggcgg ccggcggcag cggcgtcggg tcggcgccgg
       61 tggaggggaa gtcgcgcatg atgcgctcat tttccgtccg ccactttgcc tccatctcct
      121 cgagctcggc ctcgcgcatc atcgtgtcga gccccgagcg gaagtggcgg gccatgccac
      181 gcgcgcgccc gacccaatgg ccgacggtcc gcaacacgcg cggcaggtcc ttgggcccga
      241 tcaccaccaa agcgaccagc gccacgatca acagctcggt gggtgcgata tcgaacatgc
      301 gcccgcgctc agcggccggt cagggctcgc gccgatcctc gggatcggcg gtgcggctgc
      361 gctcggccgc ggcgtcgacg gtgcggtcgc cctcgacgat ccggcgcggc ggcggcgggg
      421 tcgcctcctc ctcgtcggtc atgcccttct tgaagctctt gagccccttg gcgacatcgc
      481 ccatcatttc cgagaaacgc cccttgccga acagcagcaa gatgatgacc ccgacgacga
      541 gccaatgcca aaggcttaaa ccacccatat ccaatctcca ttgcgcggcg agacgcgctc
      601 ttccgcccgc ctctagcacg atccctctga aaaagatgtt gcaaatcggc gtcgtgcgga
      661 tcgcccggcc cagcctttaa tcctcgtcgc ggcccgattc cagttcgagc tggtccatgg
      721 ccgcttcgat cggatcgagc aagccggcgg cgcgcagatc gtcgatgccg ggcaggtcgc
      781 ggcggccggc gaggccgaaa tgggcgagga agccggccgt ggtcgcatag gtgagcggcc
      841 gcccgggcgc ctcgcgccgc cccgccggcc gcacccagcc ggcttccatc agcacgtcga
      901 gcgtaccctt cgagatctgg acgccgcgga tcgcctcgat ctcggcgcgg ctcaccggct
      961 cgtgataggc gatgatcgcc aacgtctcga cggcggcgcg gctgagcttg cgcggctcgt
     1021 cgcgctcgcg ccgcaggatg tgggcgaggt cggacgcggt ctggaaatgc cagcgtttgc
     1081 cgcgctcgac cagctcgatg ccgcgcccgg cataatgttc gccgaggatc gccagcgcct
     1141 cggaaacatc gacgtcgggg ccgacatagt cggcgatctc cgccgccgtc atcggattat
     1201 cggatgcgaa taaggtcgcc tccaccgagc gcacatcgtc gccgaggccg ccagtcacgc
     1261 agccctcacg taaagcggcg cgaacgggct ttcctgctcc agctccagcc gcccctgccg
     1321 ggccagttcg agcgcggcga cgaagctcga ggcgagcgcc gagcggcgat attccggatc
     1381 cggcgtctcg ggcaggaacg agcggatctc cacccagcgg acgctggtcc cgatcagccg
     1441 ctcgacccgc agcaaagcct cttcgagcgt catcaccggc cgccgcccga cgatgtgcac
     1501 ggccggctcg gtgcgggccc ggatggtgcc gtaagcggcg atgaggtcgt aaagctcgac
     1561 ctgccacgct gccttacgaa tggtgcgcag cccttccggc gcgccgcggg cgaacacgtc
     1621 gcgccccagc cggtcgcggc ccagcagccg cgcgcccgcc tcgcgcatcg ccgacagccg
     1681 ctgcagccgg agttgcagcc gcatggcgag ctcctcgggg ctgggatcgg ccccggcgtc
     1741 gcgcggcagc agcaggcagg atttgaggta ggcgagccag gccgccatca ccaaatagtc
     1801 ggcggcgatc tccagcttca gcgacttggc gtcgtggatg aaggcgagat attggtcggt
     1861 gagcgcgagg atcgagattc tggcgaggtc gaccttctgg gtccgcgcca gcgtcagcag
     1921 caggtcgagc gggccttccc agccgtcgag gtcgagcgtc agccggtcgg ccgcggccac
     1981 cgccggccgc tcgtcgaaat cgtcgtcaaa gtccacgcga ggcagcttat gccgagcggc
     2041 cgcgcttgcg aagcccctct atgcgtaagc gagcaacgcg tcgcgcttgg cggccagctc
     2101 ggcataatcg gcgctcgcgc cggcgtcggc gaccgtggcc atcgcccgat tgagccgctc
     2161 acgccctttt tcgctgatct ccccaagtgc gctggcgacg ctcaccattt cgtccatgtc
     2221 gcccgagcaa tgcagcgcga ggtcgcagcc cgcctcgacc acgccgcgcg cgcgcgcgcc
     2281 gaagccgccg ctgagcgcgt gcatgccgag atcgtccgac atgagcaggc cgtcatatcc
     2341 gatccgcccg cggatcacgt ccgcgattac
//
LOCUS       DASVRK010000229         5272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_234227, whole genome shotgun sequence.
ACCESSION   DASVRK010000229 DASVRK010000000
VERSION     DASVRK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234227"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1339
                     /locus_tag="VF589_09100"
     CDS             257..1339
                     /locus_tag="VF589_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257780.1"
                     /translation="MARELSEIADDGAAFLFAAGCLRAVLGLAVSDRLRSLGAAARPF
                     PPSTWSILTMNGILARPRLLGLICAAAAVGLGIAYMSAAGAPSNYRLMNLAALVLGAT
                     AWLALGRVAGSSLAVAGPVTFALAAALLLTALFGSAADGASRWVSLGPLHLQVSLIVL
                     PAMLVLYARRPDSAGTAGMIVAALALALQPDRAMAGVLAAALLALLASKRGGLPLLAA
                     AGAILAFGWTFLSPDALPAVPYVDRILYTAFDVHLLAGLAVVTGAAALVLPAAAAWTL
                     ASERPALLAFGGCWLAIVVAAALGNYPTPLVGYGGSAVLGYLLSVALLPNGAREARPV
                     RLPASSGTDGRSSDPGISELRAPSLT"
     gene            complement(1336..4890)
                     /locus_tag="VF589_09105"
     CDS             complement(1336..4890)
                     /locus_tag="VF589_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8257781.1"
                     /translation="MALNDIAAPPGAAGFLAGGGEAGAMLRSFDWTASPLGPPEGWPQ
                     PLKTLVGVMLAANQPMFLAWGPTRTFLYNDAYAEILAAKHPAALGGDFLEVWHEIRGD
                     LLPIVEEAYAGRPVQMDDIELLMHRRGYPEETHFAFSYTPVRDEAGAVAGFFCPCVEI
                     TEQVFAGRRQAFRLALEQRLRDLSGASEVIAVASEALGQYLDVAQVAYAEVEPDGDTA
                     IIEREWNNGEMASNAGRHRLDDFGPAFIADLRRGDTIAIPDVRLDPRTSSPEALAAFA
                     KTSIRAFLNVPVVKAGRLAAVLAVHSQIPRAWTAEEIVLTEEIAERTWAAAERARAEA
                     ELRTSETRLQSSLRAAERKAAELGAVLESMPDAVYIGTAEGITHANRPALEQLGFASY
                     EDLNRHVATLAEEIQTRDAETGALIALEEQAFTRAFRGERVIQDVRVRHRITGEDRIV
                     RCAAAPVTMDGQVIAAVAVNTDITEAKLAEAELRSSEARLAFLDRLAAETASLANADA
                     VLATTTRLLGEHLKLSVCAYADMDEDEDGFTIRGDWAAAGSTSIVGHYSLADFGKLAV
                     KNLGAGLPLVVNDNLRELALEEAATFQSIGIAATICMPLVKEGRLTALMAIHDRVPRI
                     WTEAELSLLREVTARSWAHVERVAAIAELRASEARYRTLFETVDAGFCVVEMIFDESG
                     RPVDYRFVEANPAFERQTGLNDAVGRTAGELVPDLEPHWFELYGRVALTGEATRFEEG
                     STAMGRWFDVHALRVGDPDARRVAILFNDISKRRAAEEGLRELNDTLEQRVAERTAEL
                     EQAQEALRQSQKLEAMGQLTGGVAHDFNNLLTPIVGSLDMLVRRELGSERERRLIDGA
                     LQSAERAKTLVQRLLAFARRQPLQPSAVDIKALVGGMAELIASTSGPKIDVRVRLPDD
                     LPAAVADANQLEMAVLNLAVNARDAMPEGGVLTLSAAAETVGPGRRSALRPGDYVRLS
                     VEDTGIGMDEATLARAVEPFFSTKGIGKGTGLGLSMAHGLASQLEGGLTLSSRPGEGT
                     RVDLWLPVSLVDAKSEGQTVGARARPAGRGTALLVDDEDLVRMSTADMLMDLGYAVVE
                     AASAEAALQLVEDGLGPDLLVTDHLMPGMTGLELVQTLRAERPGLPVLIISGYAEAEG
                     IASEFARLTKPFRIAELGESLSGLSAAEQGAATSEPCG"
     gene            complement(4931..>5272)
                     /locus_tag="VF589_09110"
     CDS             complement(4931..>5272)
                     /locus_tag="VF589_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257782.1"
                     /translation="AALLAQAIGDMPSTDFDLAKFKPRPVECGSAGLGGEIVVCGRRR
                     PEPPWLAPRAGKAALPPAEIKLLGDMSVGVEAVQRDTPAFAGEQTLPGGFPPSLQKLH
                     PGPNPVYRGRI"
BASE COUNT          717 a         1898 c         1817 g          840 t
ORIGIN      
        1 cgcagggtta tctggaggcc gagtggcaac agcccgccca aagcggccgc ccgccgcgtc
       61 acgcctaccg gctgacggcc gcgggccagc ggctcgccag ggccgaggcg gcgccaaccg
      121 gcgaggctgc gacgcttccg aactggcggc cggggcggac cgcatcgtga cggcgcttgc
      181 ccgccgcctc gcgtcgcgca gcttggcggc agtgtcgcat ctgctcccac cgcatctgtc
      241 gccctgggct cgcgcaatgg cgcgcgagct gtccgaaata gccgatgacg gggccgcctt
      301 cctgttcgcg gccggctgcc tgcgtgccgt tctcgggctg gccgtcagcg accggcttcg
      361 gtcgctcggg gccgcggccc gcccattccc accttcaacc tggagcatcc tcaccatgaa
      421 cggcattttg gcgcgacctc gtttgctcgg cctcatctgc gcggcggccg ccgtcggtct
      481 gggcatcgcc tacatgtcgg cggcgggggc gccgtccaac tacaggctga tgaacctcgc
      541 agccctggtg ctcggcgcga cggcatggct cgcgctcggc cgggtcgcgg gttcatccct
      601 cgccgttgcc ggaccggtga cgttcgcgct cgcggccgcg ctgctgctga cggcgctgtt
      661 cggctccgcg gccgacggcg cctcgcgatg ggtgagcctc gggccgctcc acctgcaggt
      721 cagcctcatc gtcctgcccg ccatgctcgt cctctacgcg cgccgaccgg actcggccgg
      781 gacggccggc atgatcgtcg ccgccctggc gctggccctg cagcccgacc gtgcgatggc
      841 cggcgtgctg gccgcggcgc tcctggccct gctcgcctcg aagcggggcg ggcttccgct
      901 gctggccgcg gccggcgcaa tccttgcctt cggctggact ttcctcagcc ccgatgcgct
      961 cccggcagtg ccttatgtgg atcgcatcct ctacaccgcg ttcgacgttc acctgctcgc
     1021 cggcctcgcc gtggtgaccg gagcggccgc actcgtcctc cccgcggcgg ctgcgtggac
     1081 gctggcgagc gagcggccgg cgctgctggc gttcggcggc tgctggctcg ccatcgtcgt
     1141 ggcggcggcg ctcggcaatt acccgacccc gctcgtcggc tatggcggga gcgcggtact
     1201 cggctatttg ctcagcgtcg cgctgttgcc gaacggcgcc cgtgaggccc gccctgtgcg
     1261 tctgcccgcg tcgtccggaa cggacggccg gagctctgac cccggcatct cagagctgcg
     1321 cgcgccgagc ctgacttagc cgcagggctc gctggtcgcc gcgccctgct cggcggcgga
     1381 taggccggag aggctttccc cgagttcggc aatgcggaac ggtttggtca ggcgcgcgaa
     1441 ttcggaggca atgccctccg cttccgcata gccggagatg atgaggaccg ggaggccggg
     1501 gcgctcggcg cgaagcgtct ggaccagctc cagcccggtc atgcccggca tgaggtgatc
     1561 ggtgacgagg aggtcgggac cgagtccgtc ttcgaccagc tgcagcgccg cttcggccga
     1621 ggccgcctcg accacggcat agcccagatc catcagcatg tcggccgtgc tcatccgcac
     1681 cagatcctcg tcgtcgacca gcaaagccgt gccgcggccg gccggccggg cgcgcgcgcc
     1741 gacggtctgc ccctccgact tcgcatccac cagactgacc gggagccaga ggtcgacccg
     1801 ggtgccctcg ccggggcggc tcgagagcgt cagcccgcct tcaagctggg aggcgaggcc
     1861 gtgcgccatc gacagcccca gcccggtgcc cttgccgatg cccttcgtcg agaagaacgg
     1921 ctcgaccgcg cgggcgagcg tcgcctcgtc catgccgatg ccggtgtctt ccacggagag
     1981 gcggacataa tcgcccggcc gcagcgccga ccggcggccc ggccccaccg tctcggcagc
     2041 ggccgagagg gtgagaacgc cgccctcggg catggcgtcg cgcgcgttca cggccaaatt
     2101 cagcaccgcc atttcgagct ggttcgcgtc ggccaccgcc gccggaagat cgtccggaag
     2161 cctgacccgc acgtcgatct tggggcctga ggtgctggcg atgagctcgg ccatgccgcc
     2221 gaccagcgcc ttgatgtcga cggcgctcgg ctgcagcggc tgccgccttg cgaaggccag
     2281 cagacgctgg accagggtct tggcgcgctc ggcggactgc aaggcgccgt cgatcaggcg
     2341 ccgctcccgc tcgctgccga gttcgcggcg cacgagcatg tcgaggctgc cgacgatcgg
     2401 cgtcagcaga ttgttgaaat cgtgcgccac gccgccggtg agctggccca tcgcctccag
     2461 cttctggctc tgccgcaacg cctcctgcgc ctgttcgagc tccgccgtgc gctcggcgac
     2521 gcgctgctcg agcgtgtcgt tgagctcgcg caggccctcc tcggccgccc ggcgcttgga
     2581 gatatcgttg aacaggatcg cgacccggcg ggcgtcggga tcgccgacgc gcagcgcatg
     2641 aacgtcgaac cagcggccca tcgcagttga accctcctcg aaacgcgtcg cctcgccggt
     2701 gagcgcgacc cgcccgtaaa gctcgaacca gtgcggctcg aggtcgggga ccaattctcc
     2761 ggcggtcctg ccgaccgcgt cattgagccc ggtctgccgc tcgaaagccg ggttcgcctc
     2821 gacgaaacga tagtcgaccg ggcgcccgct ctcgtcgaag atcatctcga cgacgcaaaa
     2881 gcccgcatcg accgtctcga acaaagtgcg gtaacgcgct tcgctggcgc gcaattccgc
     2941 gatcgccgcg acccgctcga catgcgccca ggaccgcgcc gtcacttcgc ggagcaggct
     3001 cagctccgcc tcggtccaga tgcggggcac gcggtcgtgg atcgccatca aagcggtgag
     3061 gcggccttcc ttgaccagcg gcatgcagat cgtggcggcg atgccgatgc tttggaaggt
     3121 cgcggcttcc tccagcgcca gctcgcgcag attgtcgttg acgaccagcg gcagtccggc
     3181 gcccagattc ttcaccgcca gcttgccgaa atcggcgagg ctgtaatggc cgacgatgct
     3241 ggtcgacccc gccgccgccc agtcgccgcg gatggtgaag ccgtcttcgt cctcgtccat
     3301 gtcggcatag gcgcagaccg acagcttcaa atgttcgccc agcagccgcg tcgtcgtggc
     3361 gaggaccgca tcggcgttgg cgagcgaggc ggtttcggcc gcgagccggt cgaggaaagc
     3421 gagccgggcc tcactgctgc gcagttcggc ttcggcgagc ttggcctcgg tgatgtcggt
     3481 gttgaccgcc accgcggcga tcacctgccc gtccatggtg accggcgccg ccgcgcagcg
     3541 cacgatgcgg tcttcgccgg tgatccggtg ccgcacgcgc acgtcctgga tgacgcgctc
     3601 gccccggaac gcgcgcgtga aggcctgttc ttcgagcgcg atcagcgctc cggtttcggc
     3661 gtcccgggtc tgaatctctt cggccaacgt ggcgacgtgg cggttgagat cctcgtagct
     3721 ggcgaagccg agctgctcca gcgcgggccg gttggcgtgg gtgattccct cggcggtgcc
     3781 gatatagacg gcgtccggca tgctctcgag cacggcgccg agctcggccg ccttgcgctc
     3841 cgccgcccgc aaggatgact gaagccgcgt ctcgcttgtc cttagctccg cctcggcgcg
     3901 cgcccgttcg gcggccgccc aggtgcgctc ggcaatctct tccgtgagca cgatctcctc
     3961 tgccgtccag gcgcgcggta tctggctgtg gaccgcgaga accgcggcca gccgccccgc
     4021 cttgaccacc ggcacgttca ggaaggcgcg gatcgaagtc ttcgcaaagg cggcgagcgc
     4081 ctcgggcgac gaggtgcgcg ggtcgagccg cacgtcgggg atggcgatcg tgtcgccacg
     4141 ccggaggtcg gcgatgaagg ccgggccgaa atcgtcgagc cggtgacggc cggcgttgct
     4201 cgccatctcg ccattgttcc actcgcgctc gatgatcgcg gtgtcgccgt ccggctcgac
     4261 ctcggcatag gcgacctgcg ccacatcgag gtattggccg agcgcctcgg acgcgacggc
     4321 gatcacctcg cttgcgccgg acaggtcgcg cagccgctgt tcgagcgcga ggcggaacgc
     4381 ctgccgccgc ccggcgaaca cctgctcggt gatctcgacg caggggcaga agaagccggc
     4441 caccgcgccc gcctcgtcgc gcaccggcgt gtaggaaaag gcgaagtgcg tttcttccgg
     4501 atagccgcgg cggtgcatga ggagctcgat gtcgtccatc tggaccggcc ggccggcata
     4561 ggcctcctcg acgatcggca gcaggtcgcc gcggatttcg tgccagacct cgaggaagtc
     4621 tccgccgagg gcggccggat gcttggcggc caggatctcg gcataagcgt cgttgtagag
     4681 gaatgtgcgc gtcggtcccc aggcgaggaa catcggctgg ttggccgcga gcatgacgcc
     4741 gaccaaagtc ttcaacggct gcggccaccc ttcgggcggc cccagcggcg aggcggtcca
     4801 gtcgaagctc cgcagcatcg caccggcttc gccgccgccg gccagaaagc ctgcggcccc
     4861 cggcggggcg gcgatatcgt tcaacgccat aacagcaacg ctcccgacgg cggtctatcc
     4921 gactgcggct ctagattcgc ccgcgataga ctgggttagg gccggggtga agcttttgga
     4981 gcgagggagg aaatccgccg ggcagcgtct gttccccggc gaaagccggg gtatcccgtt
     5041 gcacggcttc gacccctacg gacatatcgc cgagcagctt gatctccgcc ggtggcagcg
     5101 cggccttccc cgctcggggc gccaaccaag gcggctctgg acgccgtctg ccgcagacga
     5161 cgatctcacc ccctaacccc gccgagccgc attccacggg ccggggcttg aactttgcca
     5221 gatcgaagtc ggtgctcggc atgtcgccga tcgcctgtgc gagaagggca gc
//
LOCUS       DASVRK010000230         7116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_234520, whole genome shotgun sequence.
ACCESSION   DASVRK010000230 DASVRK010000000
VERSION     DASVRK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234520"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VF589_09115"
     CDS             <1..470
                     /locus_tag="VF589_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257783.1"
                     /translation="MLVGKWTYRSFRNDPSLVGGDAAAALGLIFGEGVFDLDMDGEDR
                     FRGGLGMGTGYALTLSGTVLRGAGSALEFAIVGLGLQGTATEGWRYDYRCNPGYLWPE
                     GVEQVPSLVGTVVRVNPQGPASPAGYTGSFIAVRQPEARAPRTLRRSALTVGL"
     gene            987..1526
                     /locus_tag="VF589_09120"
     CDS             987..1526
                     /locus_tag="VF589_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257784.1"
                     /translation="MSEPMPARLPIDVLLEEVLDGGGRAFVPVPVRARRDGWTPERQR
                     GFIGRLALCGCVSRSARAVGMTKKSVYNLRERPDAGSFAAAWDRALGWGDRRMDDIAI
                     ERALHGEVRPYFYGGVKCGESVRHDNRLLLAALNRQDRRDGTANCPASIDAKEFEDLL
                     KQLHHIPADTENKGDFPPI"
     gene            1589..2035
                     /gene="queF"
                     /locus_tag="VF589_09125"
     CDS             1589..2035
                     /gene="queF"
                     /locus_tag="VF589_09125"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX8257785.1"
                     /translation="MNPIHLGQSSPLPASPEEARLDYVPNPRPGRAYLVRFAAPEFTS
                     LCPVTGQPDFAHIVIDYAPGETIVESKSLKLFLGAFRNHCGFHEDVTVGIAERLVAEM
                     KPLWLRIGGYWYPRGGMPIDVFWQSGDPPAGLWVPDQGVAAYRGRG"
     gene            2038..3078
                     /locus_tag="VF589_09130"
     CDS             2038..3078
                     /locus_tag="VF589_09130"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF006083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX8257786.1"
                     /translation="MDNPRIVVHGAGSVGCFIGGAWLAAGAKVGFLGRERIAAELAEH
                     GLSLSDQDGWRIHLARDLIDYSAKPAVLKTADIVALAVKSSGTEAAAREIARHARPGT
                     PVISFQNGIGNPDALKRLLPKMRVLRGMVPYNVVRLGPGRYHRASWGDLMAEDDAATQ
                     ALAAKIGSRPGRLLLAKDMLPVAWGKLLLNLNNAVNALSGRTLLEELRERDYRRVLAA
                     AMIETLELLTAAGIEPAKMGPIPPRLLPHAIGANDFMVKNLLLRVMKIDPKARSSMAD
                     DLAAGRPTEIDHLNGEVVKLARSLGREAPVNAAITALIKEAETGNERSWSPRELYIHV
                     LQGHKRAALFGY"
     gene            complement(3082..5028)
                     /locus_tag="VF589_09135"
     CDS             complement(3082..5028)
                     /locus_tag="VF589_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX8257787.1"
                     /translation="MASRPLPELTVRGVLLGGVITLLFTAANVYMGLRVGLTFATSIP
                     AAVISMAVLRMFATSNILENNIVQTVASAAGTLAAICFILPGLVMVGWWQGFPFWTTA
                     FVCALGGLLGVMFSIPLRRALVADSPLPYPEGRAAAEVLKIGEGSRAGDAESNAGLKI
                     MAAATLVSAGFAALVYTRLAVAEATRFFKVGASATGIAGGLSFALLGAGHLVGLTVGL
                     AMFAGLVIGWGVATPWLTALVPQTGTVEEFAIGIWATQVRFIGAGVIGVAAIWTLLRI
                     IGPILKGIASAMAASRARRSGGEGLPLQERDIPVGIVALVSGAAMIPIAFLLADFASG
                     GPLAGITVPLVAVTLVFILLAGAFIASVCGYMAGLIGASNSPISGVGILSILAASVLL
                     GIFFAPGLGEGGEQALIAYALFTTAIVFGVATISNDNLQDLKTGQLVGATPWRQQVAL
                     IFGVMFGSLVIPPVLDLLNAAYGFAGAPGAGANALPAPQAALVSALASGVLGGDLDWS
                     LIGIGAAVGVGIVIVDELLSRTGRLRLHPLGVGIGIYLPMATTMTVVVGAVVGHLYDR
                     RVERRTANAERAKRMGVLAATGMIVGESLFNVGYAALVVGSGSEAPIALVGDEFAGAA
                     AVGGTLLFALLAWWLYRRSARAGS"
     gene            complement(5031..5543)
                     /locus_tag="VF589_09140"
     CDS             complement(5031..5543)
                     /locus_tag="VF589_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257788.1"
                     /translation="MAILNPEHLLAQANRLIQPPGAGPPRQVDLRRAISAAYYAAFHT
                     ALIAAADEFVGVTLRHTREYALVFRSVDHRGLKLVCEEVVKSTPKPGYAAYIPTGGFG
                     AEIIDFADAFPNLQEQRHSADYDPLGRFRTSDAKSVIVTARAIIGRFRAASAADRKAF
                     LFLLLFPPRR"
     gene            complement(5524..5811)
                     /locus_tag="VF589_09145"
     CDS             complement(5524..5811)
                     /locus_tag="VF589_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257789.1"
                     /translation="MSDVRAAARKAAISSLNDDFVEEVLVEPAIGSDGDEILRITIVL
                     NEGAIDKIDESQFLTTLLSIKRTLRDVGDERGSVVTYATTAELEDDGDTES"
     gene            complement(5864..>7116)
                     /locus_tag="VF589_09150"
     CDS             complement(5864..>7116)
                     /locus_tag="VF589_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX8257790.1"
                     /translation="WTLTAFPVEFLSLGGLGPGVPVRATMTDFGPTLLAKVLDLNATQ
                     ESSLGLVFRYADDAGLPLLDLKDLRAVISWLVSDEGKPDLKGLGGLSASTAGVLLREL
                     IGLEELGGDVFFGEPAWEPADLMRTAADGRGVISAVELPAVQDRPALFSTFLMWLLAE
                     LFEDLPEVGDLDKPKLVFFFDEAHLLFTGASKAFLESVTQTVRLIRSKGVGIFFVTQN
                     PIDIPAEVAGQLGTRIQHALRAYTPRDQRAIKAAAETFRVNPKLDVETAITELKVGEA
                     LVSVLQEDGSPSPVERTLVKPPRSRLGPLTERERAVIQSISPAAGKYEAAVDRESAEE
                     ILKARTEAAAAAAAADKSKAEAAKQEAERLKVEARQRRAERADPSFQDQMVRQVGKAV
                     QRQVVNRIAGQLVRGLLGGLFKGR"
BASE COUNT         1188 a         2527 c         2332 g         1069 t
ORIGIN      
        1 ggatgttggt tgggaaatgg acctaccgta gcttcaggaa cgatccttcc ctcgtcggcg
       61 gggatgccgc cgccgcgctc ggcctgattt tcggtgaagg agtgttcgac ctcgacatgg
      121 acggcgagga tcgctttcgc ggcggcctcg gtatgggaac cggctacgcg ctgacgcttt
      181 ccggaaccgt cctgcgcggc gccgggagcg ccttggaatt cgccatcgtc ggcctcggcc
      241 tccaggggac ggcgacggag ggctggcgtt acgattatcg ctgcaacccc ggctatctct
      301 ggcccgaagg ggtggagcag gtcccgtcgc tggtcggcac cgtcgtgcgg gtcaacccgc
      361 aggggccggc ctcgcccgcc ggctacacgg gatccttcat cgcggtgcgc cagccggagg
      421 cgcgggcgcc gcgcaccctg cgccgctcgg ctctgacggt ggggctgtga gcccgcggct
      481 cttgcaccgg gttcgtctgg cggcgtgcgg cccctccgcc ctggcggctc ttttcctcgc
      541 cgcatcgccc gctttggctg cgggggaacc gcgcatggct gagattcgca ccggcgacgg
      601 ccccgcctgg gtagcgctcc ccgacgatga atcgctgccc tcgcgtggcc gaaactatct
      661 cgaccatggc ggctgcgccc atgcggtctg cccgcgcggt tccgctcccg ggcgcaagga
      721 ccgccgagcg gatcgccctt agggatcacg cgcgcagccc tccagatcga ccgcacccgc
      781 actacacgga ctacataacc gccactcttt acccttggct cgaagcgccg tcatcacagg
      841 acgggtccac acgaaatcac cgccttcttc atccgcgggc agtcgttcga gcgtgtcgac
      901 agccatccgg acgatactcg gcctgtcctc ccgcaatgca attttctgtt ttcctacaga
      961 tcaccatatc ggctaagttg attcgcatgt ctgaaccgat gcccgcccgc ctgcctatcg
     1021 acgtcctcct cgaagaagtg ctcgacggtg gcggccgtgc cttcgttccg gtcccggtgc
     1081 gcgcacggcg cgacgggtgg acgccggaac ggcagcgggg gtttatcgga cggctcgctc
     1141 tgtgcggctg cgtgtcgcgc tcggcgcgcg cggtcggcat gaccaagaag agcgtctaca
     1201 atctgcgcga gcggccggat gcggggagct tcgccgccgc ctgggaccgg gcgctcggct
     1261 ggggcgaccg tcgaatggac gatatcgcga tcgagcgggc cttgcacggc gaagtccgcc
     1321 cctatttcta cgggggcgtc aaatgcggcg agagcgtgcg gcacgacaac cggctgttgc
     1381 tggctgccct gaaccggcag gaccgccggg acggcacggc gaactgcccg gcgagcatcg
     1441 acgccaagga attcgaggat ctgctcaagc agcttcacca tattcctgcc gacactgaaa
     1501 acaaaggaga tttcccgccg atatgagggg gttttggtgt acttccagac gcgatgacag
     1561 cggcgccggt tccgactaag gcgaggcgat gaacccgatc catctcggcc agtcgagccc
     1621 gctccccgcc tcacccgagg aagcccggct cgattatgtc cccaatcccc gccccggccg
     1681 cgcctatctg gtccgcttcg ccgcgcccga attcacctcg ctctgcccgg tcaccggcca
     1741 gcccgatttc gcccatatcg tgatcgatta cgcgcccggc gagacgatcg tcgaatccaa
     1801 gtcgctgaag ctgttcctcg gcgcgttccg caaccattgc gggttccacg aagacgtgac
     1861 ggtcggcatc gccgagcggc tcgtcgccga gatgaaaccg ctgtggctgc gcatcggcgg
     1921 ctattggtat ccgcgcggcg gcatgccgat cgacgtcttc tggcaatcgg gcgatccgcc
     1981 cgccggtctg tgggtgccgg accagggcgt cgccgcctat cgcgggcggg gctaggcttg
     2041 gacaatccgc ggatcgtcgt ccacggcgcc ggctcggtcg gctgcttcat cggcggcgcc
     2101 tggctggccg cgggcgccaa ggtcggcttc ctcggccgcg agcggattgc cgccgagctc
     2161 gccgagcatg gcctgtcgtt gagcgatcag gacggctggc gaatccacct cgcccgcgac
     2221 ctcatcgact attcggccaa gcccgccgtg ctgaaaacgg ccgacatcgt cgcgctcgcg
     2281 gtcaagagca gcggcaccga agcggcggcg cgcgagatcg cccgccacgc ccgccccggc
     2341 acgccggtga tcagcttcca gaacggcatc ggcaatccgg acgcgctgaa gcggctgctc
     2401 ccgaagatgc gcgtgctgcg cggcatggtc ccctacaatg tcgtccgcct gggccccggc
     2461 cgctaccacc gcgctagctg gggcgacctc atggccgagg atgacgcggc cacccaggcc
     2521 ctcgccgcga agatcggcag ccgcccgggc cggctgctcc tcgccaagga catgctcccc
     2581 gtcgcctggg gcaaattgct gctcaacctc aacaatgcgg tcaacgctct gtccggccgc
     2641 accttgctcg aggaactacg cgagcgcgac tatcgccgcg tcctcgccgc cgcgatgatc
     2701 gagacgctcg agctgctgac cgccgccggc atcgagcccg ccaagatggg gccgatcccg
     2761 ccgcggctgc tcccgcacgc gatcggggcc aacgacttca tggtcaagaa cctgctcctc
     2821 cgggtgatga agatcgaccc caaggcgcgc tcgtcgatgg ccgacgatct cgccgccggc
     2881 cgcccgaccg agatcgacca tctgaacggg gaagtggtga agctcgcgcg ctcgctgggc
     2941 cgcgaggcgc cggtcaacgc cgccatcacc gcgctgatca aggaagcgga gacggggaac
     3001 gagcgaagct ggtccccgcg cgagctctac atccacgtgc tccagggcca caagcgcgcc
     3061 gccctgttcg gctactgacg ctcagctccc ggcgcgcgcg ctgcggcgat agagccacca
     3121 agcgagcagg gcgaacagca aggtgccgcc gaccgccgcc gcgccggcga actcgtcgcc
     3181 gaccaaagcg atcggcgctt cgctgccgct gccgaccacc agcgccgcat agccgacatt
     3241 gaacaggctc tcgccgacga tcatgccggt cgcggcgagc acgcccatcc gcttggcccg
     3301 ctcggcattg gccgtgcggc gctcgacgcg gcggtcgtag agatggccca ccaccgcccc
     3361 gaccacgacc gtcatggtcg tcgccatcgg caaatagatg ccgatcccga ccccgagcgg
     3421 gtggagccgc agccgcccgg tccggctcag caattcgtcg acgatcacga tcccgacgcc
     3481 gaccgccgcg ccgatcccga tcaggctcca gtcgaggtcg ccgccgagca cgcccgaggc
     3541 gagcgccgac accaaagccg cctgcggcgc cggcaaggca ttcgcgcccg ccccgggagc
     3601 gccggcaaag ccgtaggcgg cgttgagcag gtccagcacc ggcggaatca ccagcgatcc
     3661 aaacatcacc ccgaagatca gggcgacctg ctgccgccac ggcgtcgcgc cgacgagctg
     3721 cccggtcttc agatcctgca gattgtcgtt ggagatggtg gcgacgccga acacgatcgc
     3781 ggtcgtgaac aaggcgtagg cgatcagcgc ctgctcgccg ccctcgccca gacccggcgc
     3841 aaagaagatg ccgagcagca ccgaggcggc gaggatcgag aggatgccga cgccggagat
     3901 cgggctgttc gaggctccga tcaggcccgc catatagccg cagaccgagg cgatgaaggc
     3961 gccggccagc aggatgaaca ccaaagtcac cgccaccaac ggaaccgtga tccccgccag
     4021 cggcccgccc gacgcgaagt cggcgagcag aaaggcgatc gggatcatcg cggcgccgga
     4081 gaccaaagcg acgatgccga ccggtatgtc gcgctcctgc agcggcagcc cctcgccgcc
     4141 cgagcggcgc gcgcgcgagg ccgccatcgc gctggcgata cccttgagga tcgggccgat
     4201 gatccggagc aaggtccaga tcgccgccac gccgatcacg ccggcgccga tgaagcgcac
     4261 ctgggtcgcc cagatcccga tcgcgaattc ctcgaccgtc ccggtctgcg gcaccaaagc
     4321 ggtcagccac ggcgtcgcca cgccccagcc gatcaccagc ccggcgaaca tggcgagccc
     4381 gaccgtcagc ccgaccagat gccccgcccc gagcaaggcg aagctgaggc cgcccgctat
     4441 cccggtcgcc gacgccccga ccttgaagaa gcgcgtcgcc tcggcgaccg ccagtcgggt
     4501 atagactagg gccgcgaaac ccgccgagac caaagtcgcc gcggccatga tcttgagccc
     4561 ggcattgctc tcggcgtcgc cggcgcgcga gccctcgccg atcttcagca cttcggcggc
     4621 ggcgcgccct tccggataag gcagcggcga atcggcgacc aaagcgcgcc gcagcgggat
     4681 cgagaacatc acgccgagca gcccgcccag cgcgcagacg aaggcggtgg tccagaacgg
     4741 aaagccctgc caccagccga ccatcaccag gcccggcagg atgaagcaga tcgccgccag
     4801 cgtgcccgcc gccgaagcga cggtctggac gatattgttc tcgaggatgt tggaggtggc
     4861 gaacatccgc agcaccgcca tcgagatcac cgccgccggg atcgaggtcg cgaaggtgag
     4921 gccgacgcgc aggcccatat agacgttggc cgcggtgaac agcagagtga tgaccccgcc
     4981 cagcaggacg ccgcggacgg tgagttcggg gaggggacga gaagccacca tcatcgcctc
     5041 ggcgggaaga gcaaaaggaa aaggaacgcc tttcggtccg ctgccgacgc cgcccgaaag
     5101 cggccgatta tggcgcgcgc ggttacaatg acgcttttcg cgtccgaggt gcgaaacctc
     5161 ccgagcgggt catagtccgc cgaatgccgc tgctcttgga gattcgggaa agcgtccgca
     5221 aaatcgatga tctccgctcc aaagccgccc gtcgggatgt aggcggcgta gcctggcttg
     5281 ggggtcgact tcacgacttc ctcgcagacc agcttcaatc cacggtgatc cacactccgg
     5341 aagacgagcg catattcccg cgtgtgcctc agcgtcactc caacgaactc gtccgccgcg
     5401 gcgatcagcg cagtgtggaa ggctgcataa taagcagccg aaatagctcg ccgtaagtcc
     5461 acctggcggg gcggacccgc accgggcggc tggatgagac ggttagcctg agcaagcaga
     5521 tgctcaggat tcagtatcgc catcgtcttc cagttccgcg gtcgtggcgt aggtgacgac
     5581 cgacccgcgc tcgtctccca cgtctcgcaa ggtccgcttg atcgataaca aagtcgtgag
     5641 gaactggctc tcgtcgatct tgtcgatcgc accttcgttg aggacgatcg tgatgcgcag
     5701 gatctcatcg ccgtcggacc cgatggccgg ctcgaccagg acttcctcga cgaaatcgtc
     5761 attgagagag cttatggcag ccttgcgtgc ggcggctcta acgtcgctca tcgcggtcat
     5821 gccctgactt tagccaatcg gccgtgtgag cgcattctaa ttctcagcgc cccttgaaaa
     5881 gccccccgag cagcccgcgc acgagctgcc ccgcgatccg gttcaccacc tggcgctgca
     5941 cggccttgcc gacctggcgc accatctggt cctggaagga cgggtcggcc cgctcggccc
     6001 ggcgctgccg agcttcgacc ttgagccgct ccgcttcctg cttggcggcc tcggccttgg
     6061 atttgtccgc cgccgccgcg gcggccgccg cttcggtgcg ggccttcaat atctcctcgg
     6121 cgctttcgcg atcgaccgcg gcctcgtatt tccccgcggc gggggagatg gactggatca
     6181 ccgcccgctc gcgctccgtc agcggcccca gccgcgaacg gggcggcttc acaagcgtcc
     6241 gctccaccgg cgacggcgag ccgtcctcct ggagcaccga gaccaaagct tcgccgacct
     6301 tcagctcggt gatcgcggtc tcgacgtcga gcttcgggtt gacccggaac gtctcggcgg
     6361 ccgccttgat cgcccgctgg tcgcgcggcg tataggcgcg cagcgcatgc tggatgcggg
     6421 tgccgagctg acccgccact tcggccggaa tgtcgatcgg gttctgcgtc acgaagaaga
     6481 tgccgacgcc cttcgaccgg atcagccgca ccgtctgggt caccgattcg aggaacgcct
     6541 tgctcgctcc cgtgaacagc aggtgcgcct cgtcgaagaa gaagaccagc ttcggcttgt
     6601 cgaggtcgcc gacctcgggc aggtcctcga acagctcggc gagcagccac atgaggaagg
     6661 tggagaacag cgcgggccgg tcctggacgg cgggcagctc gacggcgctg atcacgccgc
     6721 gcccgtcggc ggcggtgcgc atcaggtcgg ccggttccca cgcgggttcg ccgaagaaga
     6781 cgtcgccgcc gagctcctcg aggccgatga gttcgcgcag cagcaccccc gcggtgctcg
     6841 cggacagccc gccgaggccc ttgaggtccg gcttgccctc gtcgctcacc agccagctga
     6901 tgacggcgcg gaggtccttg aggtccagca gcgggaggcc ggcgtcgtcg gcgtagcgga
     6961 agaccagccc gagcgagctc tcctgcgtgg cgttgaggtc gagcaccttg gccagcagcg
     7021 tcggcccgaa gtcggtcatg gtggcccgca ccggcacgcc cgggccgagg ccgccgaggg
     7081 agaggaactc caccgggaac gccgtgagtg tccagt
//
LOCUS       DASVRK010000231         6781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_356289, whole genome shotgun sequence.
ACCESSION   DASVRK010000231 DASVRK010000000
VERSION     DASVRK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356289"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VF589_09155"
     CDS             <1..536
                     /locus_tag="VF589_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257791.1"
                     /translation="PAGWRQLVVAAPREAAAAASAGLPSAAHRRLALLMIRAEAGDAL
                     EAIAATLQDDPPAVLGPHYTGREIVIGYAGPAEAAAAAIRLAAEAGAAVGGHYGVAQP
                     VRDPFGGELRIGGDAVALAAGAAASAPPGSACVTADFAAALAASREADVHSELIGELD
                     ARDGSEPVDLYALKPRL"
     gene            complement(531..1124)
                     /locus_tag="VF589_09160"
     CDS             complement(531..1124)
                     /locus_tag="VF589_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8257792.1"
                     /translation="MGLGRVRLYAAGAHLLRQGDRPTAIYLIILGRCHALLYGADGQM
                     VLLHELGPGDLFGALGGLDAVEEDADVVAVEEVRSFLLEAGILVGLAERHGCIGLALS
                     RILLKRLRATSTRMFERAALSASGRVCAELLRRARAGEGLAIRPLPVLAELAMQVGTT
                     RETASRTVSALERRGILRREPDALIVVAPGRLEAEML"
     gene            complement(1306..1382)
                     /locus_tag="VF589_09165"
     tRNA            complement(1306..1382)
                     /locus_tag="VF589_09165"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Pro,seq:cgg)
     gene            complement(1468..2781)
                     /locus_tag="VF589_09170"
     CDS             complement(1468..2781)
                     /locus_tag="VF589_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8257793.1"
                     /translation="MRHIAVIGSGPAGYYSAEALQKAFGEAVRIDVIDRLPVPYGLIR
                     FGVAPDHQSIKAVYRRYEKVALSGNVRFVGNVLVGRDITIAELIELYDAVILATGAPV
                     DRPLGIPGADLPGVVGSAAFVGWYNGHPEFADLDPPLDVKAAAVIGNGNVALDVARIL
                     SKTRAEFQGSDIVGHALDTLERSEIRLIHVLGRRGPHQIAMTPKELGELGYLEAAAPR
                     VEPADFPPEGDDALLEPGMRKSVGHLRSFASLPQDKPKTIEFDFFAMPIAVEGDGRAE
                     RLIVERTRLTEDLRSVGTGERYAIDCGLIVSCIGYQTPPIEGVPYEHGRGRFANHDGV
                     IEAGPDGGLYCVGWARRGPTGTIGTNRPDGYEVADKIFATLGEGVSGKFGRPGLDALL
                     DGRGVDVVTFRDWQKIEAAEAARARDGSPREKFTAIADMLAARGA"
     gene            complement(2878..3129)
                     /locus_tag="VF589_09175"
     CDS             complement(2878..3129)
                     /locus_tag="VF589_09175"
                     /inference="COORDINATES: protein motif:HMM:NF044559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257794.1"
                     /translation="MQWAFLKAAVDCAETNGELRAVAAGPFEHLLGSHGEHYIAQVES
                     RCREDGKFARMTTAAWRHRMGDDVWRRVQAIQAAVPDPL"
     gene            3527..3922
                     /locus_tag="VF589_09180"
     CDS             3527..3922
                     /locus_tag="VF589_09180"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEX8257795.1"
                     /translation="MNKAILALAAAAGLAGASSAQPPIPAPEAEASIPFAHQRIRSFR
                     SLDDRSVYIRVGSRLRGEWYHATTAVPCRELPYALAIGVDRFSTTGTLDRFSTLLVDG
                     ERCPLQSLVRSGPPPRKVKSDRYRSARNS"
     gene            complement(4071..4394)
                     /locus_tag="VF589_09185"
     CDS             complement(4071..4394)
                     /locus_tag="VF589_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX8257796.1"
                     /translation="MNKPLSVDLPHNLGAEEAKRRMQGGIGRLKDHVPGGSAQVESRW
                     EGDRMYLDVRAMGQEVTGHIDVEERKVRLELMVPPVLALFAGKIEGLIRARGTELLED
                     KSKRG"
     gene            4563..5831
                     /locus_tag="VF589_09190"
     CDS             4563..5831
                     /locus_tag="VF589_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX8257797.1"
                     /translation="MLRFHWLKRLRQSERGNVLVIGAASLPLLMGATALAVDTIQLSL
                     WKRQMQRAADSAAIAGAYALDQGTPIAAAIANDLDEHETPDATDAVATGSFTGGTQAA
                     ETCAVRGGDPCYGQSVQVTLSAAPRLPFISFFTGAASPITAKAVAALAPDGRYCMISL
                     YDGDEPGITAGGNGKVDLNCGAGTNSESDTAIVADGSSEFEASPLGSVGNIGTGNNFI
                     GNPIIRPFSRPIRDPLRHIQDPAPDPSDCTTAFDDSPGQVQEIPDGACYSSMIIQGHP
                     KFLGEVYVYGGDVTINSTARITDDGASTLILTGPDGEAGNMHVNGNAVMTLTAPTSGP
                     YSGVVLYRDRRASVATVTLNGGATLNLTGAMYFPTTDLTILGNFVLNSECLQLVGRKL
                     NFQGTTDIDNECDTNDGTDPFQISRVRIVG"
     gene            5836..6324
                     /locus_tag="VF589_09195"
     CDS             5836..6324
                     /locus_tag="VF589_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257798.1"
                     /translation="MLRGSIIWPGGLRGDDRGTSVVELGLLLPLLTMLIVNIVDLSMG
                     VSRRFEIHQAVHRTMELAAAANGFPIDAETGEYDYSALKVHAAAAAGVEEGDVDLRWW
                     LECDGVEQDEYYMQCPEGDPSARYLQVRVEAAYTPVFSISAAGLLGDQITMSAESAVR
                     IE"
     gene            6321..>6781
                     /locus_tag="VF589_09200"
     CDS             6321..>6781
                     /locus_tag="VF589_09200"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8257799.1"
                     /translation="MKQKINHFRRDQKGAGAMEFALAAPVLFTMIVGISQMGTLFFAN
                     AGLSHAVSEGARIAANFPTPADDVIKAAILDKRFGLVEENIVGEPTIVPGTDTATSMP
                     FLDISLSYEVPLNFIFFTGPEVTLTETRRVFRQIETPAPVDEDDEDEDEGE"
BASE COUNT         1112 a         2402 c         2241 g         1026 t
ORIGIN      
        1 ccccggccgg gtggcggcag ctagtcgtcg ccgcgccgcg cgaagccgcc gccgccgcct
       61 cggccggcct cccctccgcc gcgcaccggc ggctggcgct gctcatgatc cgcgctgaag
      121 cgggcgacgc gctggaggcg atcgcggcca ccctccagga cgatccgccg gccgtgctcg
      181 gccctcatta taccggccgc gagatcgtca tcggctatgc gggaccggcc gaagcggccg
      241 cggcggcgat caggctcgcc gcggaagccg gcgcggcggt cggcggccat tacggcgtcg
      301 cgcagccggt gcgcgatccg ttcggcggcg agctccggat cggcggggac gccgtcgccc
      361 tggccgccgg cgccgcagct tcggcgccgc cgggctcggc ctgcgtcacg gccgatttcg
      421 ccgccgcttt ggccgcatcg cgcgaggctg acgtgcattc cgagctgatc ggcgagctcg
      481 acgcgcgcga cggcagcgag ccggtcgatc tctacgcgct gaagccgcgg ctataacatc
      541 tcggcttcga gccgcccggg cgcgaccacg atcagcgcgt ccggttcccg gcggaggatc
      601 ccgcgccgct cgagcgcgct gaccgtgcgc gaggcggtct cgcgcgtcgt ccccacctgc
      661 attgcaagct ccgcgagcac cggcaacgga cggatggcga gcccctcgcc ggcgcgtgcg
      721 cgcctgagca attccgcgca cacccgcccg ctggcggaga gcgccgcccg ctcgaacatg
      781 cgcgtcgacg tcgcgcgcag ccgtttcagc aatatccgcg acagcgcgag cccgatacag
      841 ccgtgccgct ccgccagccc gaccaggatg ccggcctcga gcaggaatga gcgcacctcc
      901 tcgaccgcga ccacgtcggc atcctcctcg accgcgtcga gcccgcccag cgccccgaac
      961 aggtcgcccg gccccaattc gtggagcagc accatctgcc cgtccgcgcc gtagagcagg
     1021 gcatggcatc ggccgaggat gatcagatag atggcggtcg gccggtcccc ctgccgcagc
     1081 agatgcgctc cggccgcgta gagccggacc cgccccagcc ccaatatccg ttccgaaagg
     1141 tcgggagcgc agccgagggc ggccctgacg gcgttgagat cgacggaatc gaacagaagc
     1201 tcctccgcgc tttcgcccga gactaaagcg atccgtcggc aaaatacatg ggcccggcgc
     1261 caggagcggc tgccgatcca gcgatatgaa cggctgaagg taaaatggtc ggggagagag
     1321 gattcgaacc tccggcccct gcctcccgaa gacagtgctc taccaggctg agctactccc
     1381 cgaccggtgc gtccggcccc gctaagggac ccaccgggag acaggtggcg ggtctatagg
     1441 gagggcggcc ggggagcgca agcgcgctca cgcgccgcgc gccgccagca tgtcggcgat
     1501 ggcggtgaat ttctcgcgcg ggctgccgtc gcgggcgcgg gcggcctcgg cggcctcgat
     1561 cttctgccag tcgcggaagg tgacgacgtc gaccccgcgc ccgtcgagca gggcgtcgag
     1621 cccgggccgg ccgaacttgc cgctgacgcc ctcgccgagg gtcgcgaaga tcttgtccgc
     1681 cacctcatag ccgtcgggcc ggttggtgcc gatcgtcccg gtcggcccgc gccgcgccca
     1741 gccgacgcaa tagaggcccc cgtccggccc ggcttcgatc acgccgtcat ggttggcgaa
     1801 gcggccgcgg ccatgctcgt aaggcacgcc ctcgatcggg ggggtctggt agccgatgca
     1861 gctcacgatg aggccgcagt cgatcgcgta ccgctcgccg gtcccgaccg agcgcagatc
     1921 ctcggtcagc cgcgtgcgtt cgacgatcag ccgctcggcc cggccatcgc cctcgaccgc
     1981 gatcggcatg gcgaagaagt cgaattcaat cgtcttgggc ttgtcctgcg gcagcgaagc
     2041 gaagctgcgc aagtggccga ccgacttgcg catgccgggc tcgagcaggg cgtcgtcgcc
     2101 ctcgggcggg aaatctgccg gctcgacgcg cggggcggcc gcttcgagat agccgagctc
     2161 gcccagctcc ttgggcgtca tcgcgatctg gtgcggcccg cgccgcccga gcacgtggat
     2221 cagccggatt tcgctgcgct cgagcgtgtc cagcgcatgg ccgacaatgt ccgagccctg
     2281 gaattcggcg cgtgtcttgg acaggatgcg ggcgacgtcg agcgccacgt tgccgttgcc
     2341 gatcaccgcc gccgccttga cgtccagcgg cggatcgagg tcggcgaatt caggatggcc
     2401 gttataccag ccgacgaagg cggccgagcc gaccacgccg ggcaggtccg cgcccgggat
     2461 gccgagcggc cggtcgacgg gcgccccggt cgccaggatc acggcatcgt aaagctcgat
     2521 gagctcggcg atcgtgatgt cgcggccgac cagcacgttg ccgacgaacc gcacattccc
     2581 gctcagcgcc actttctcgt agcggcggta gaccgccttg atcgactggt ggtcgggcgc
     2641 gacgccgaag cggatgaggc cgtagggcac cggtagccgg tcgatcacgt cgatccgcac
     2701 cgcctcgcca aacgcctttt gcagcgcttc ggcgctgtaa tagccggcgg ggcccgaacc
     2761 gatcaccgca atatgccgca tccccgcctc ccgatgttaa gcggcgatgc tagccgaagg
     2821 gccggccgag gcaaggggcg cggcggacgg gaacgggaag gtgcctgaac cggaggctca
     2881 aagcgggtcg gggacggccg cctgaatggc ttgcacgcgt ctccacacgt cgtcgcccat
     2941 ccggtgccgc caggcggcgg tggtcatgcg ggcgaatttg ccgtcttcac ggcagcggct
     3001 ttcgacctgg gcgatataat gctcgccatg agaacctagc aaatgttcga acgggccggc
     3061 ggcgacggcg cgcagctcgc cattcgtttc ggcgcagtcc accgcggcct tcaggaatgc
     3121 ccattgcaca tccgcacctg ttcccctgcg aaggcagggg tccagacccg gccattcttc
     3181 ctggatgaag gccgatatgt ttacaccaat gagagcggcc tcaacggcgc agatgaatcc
     3241 gactggcgcg acctgtaccc gacctccgtc tgacaggctg cgcctggtct gggcccctgg
     3301 cttcgcaggg gcgcggtcag cgcccatcat cactcatcca tccggtgagc ctgtcgaacc
     3361 atgaacaggc cggattgacc ccggcaatcc tgaaacattc cggggcatgt ttcacctgct
     3421 cattctcgaa ggactggtgc actgagccgc tacgaagcgc acggggccgg cccccagccc
     3481 ccggtcagcc gccgttcacg gcgggccgtg taggaacggc ggcatgatga acaaagcgat
     3541 tctggcactt gccgcagcgg ccggccttgc cggcgcatcc tccgcgcagc ctcccattcc
     3601 cgcgcccgag gccgaggcga gcattccctt cgcgcaccag cgcatccgca gcttccgctc
     3661 gctcgacgac cgctcggtct acatccgggt cggcagccgc ctgcgcggcg aatggtatca
     3721 tgcgaccacg gccgtgcctt gccgcgagct tccctacgcc ctggcgatcg gcgtcgaccg
     3781 cttcagcacg accggcacgc tcgaccggtt cagcaccctg ctggtggacg gcgagcgctg
     3841 tccgctccag tcgctggtga ggagcgggcc gccgccgcgg aaggtcaagt ccgaccgcta
     3901 tcgcagcgcc cgcaacagct agcgcgttcg agcgtcagct tgtcccgtcc ccgttcaggc
     3961 tgagcctgtc gaagcccatt cccttctccc ccttcggagc agggaagaga ggcccttcga
     4021 caagctcagg gggaaaggat tggggcgatt ccctccaacc cttgagcgcc ctacccccgc
     4081 ttgctcttgt cctcgagcag ctcggtgccg cgggcgcgga tcaggccttc gatcttgccg
     4141 gcgaacaaag ccaagacggg cggcaccatc agctcgaggc ggaccttgcg ctcctcgacg
     4201 tcgatatggc cggtgacttc ctgacccatg gcgcggacgt cgagatacat gcggtcgccc
     4261 tcccagcgcg attcgacctg ggcgctgccg ccgggaacat gatccttgag gcggccgatc
     4321 ccgccttgca tccgccgctt ggcctcttcg gcgccgagat tgtgcgggag gtcgaccgag
     4381 agcggcttgt tcatgccgcc ggtctagccc gcgccgcccg ccgcgcacaa gcacggccgc
     4441 gacgggctct cgcgggacag ggcaaagatt cgattaagca tctcttctaa cccgcggtaa
     4501 atcaccagcg cctactccct cgtcggctga gatatttttc cggaccggtc gagggaccat
     4561 cgatgcttag attccattgg ctcaaacggc ttcgccaaag cgagcggggc aacgtcctcg
     4621 tcatcggtgc ggcctcgctg ccgctgctga tgggcgcgac cgcgctggcc gtcgacacca
     4681 tccagctgag cctgtggaag cgccagatgc agcgggcggc ggattcggcg gcgatcgccg
     4741 gcgcctatgc gctcgaccag ggcacgccga tcgctgccgc gatcgccaac gatctcgacg
     4801 agcatgagac gcccgacgcc accgacgccg tcgcgaccgg cagcttcacc ggcgggacgc
     4861 aggcggccga gacctgcgcg gtgcgcggcg gcgacccctg ctacggccag agcgtccagg
     4921 tgacgctgag cgccgcgccg cggctgccgt tcatcagctt cttcaccggc gccgccagcc
     4981 cgatcacggc caaggccgtg gccgcgctcg cgcccgacgg ccgctattgc atgatctcgc
     5041 tgtacgacgg cgacgagccg ggcatcaccg ccggcggcaa cggcaaggtc gatctcaatt
     5101 gcggcgcggg caccaactcc gaatccgaca cggcgatcgt cgccgacggc tcgtcggaat
     5161 tcgaagcctc gccgctaggc tcggtcggca atatcgggac cggcaacaat ttcatcggca
     5221 atccgatcat aaggccgttc tccaggccga tccgggatcc gctgcgccac atccaggacc
     5281 ccgcgcccga cccgtccgac tgcacgacgg ctttcgacga cagccccggc caagtgcagg
     5341 agatacccga cggcgcctgc tacagctcga tgatcatcca gggccatccg aaattccttg
     5401 gcgaggtcta tgtctacggc ggcgacgtga cgatcaactc gacggcgcgc atcaccgacg
     5461 acggggcctc gacgctgatc ctgaccgggc ccgacggcga ggccggcaac atgcacgtca
     5521 acggcaacgc cgtgatgacc ctcaccgcgc caacctcggg accctatagc ggagtggtct
     5581 tgtaccggga ccggcgcgcc agcgtggcca cggtcacgct caacggcggc gccaccctca
     5641 acctcacggg agccatgtac tttccgacca ccgacctcac catccttgga aatttcgtgt
     5701 tgaactcgga atgcctccag ctggtcggcc gcaagctcaa cttccagggc accaccgaca
     5761 tcgacaatga gtgcgacacc aacgacggga cggacccgtt ccagatcagc cgggtgagga
     5821 tcgtgggctg atccgatgct gaggggatcc atcatctggc caggcggcct tcgcggcgac
     5881 gatcgcggca ccagcgtggt cgaattgggg ctgctgctgc cgttgctgac gatgctgatc
     5941 gtgaacatcg tcgatctgtc gatgggcgtg tcgcgccggt tcgagatcca ccaggcggtc
     6001 caccgcacga tggagctcgc cgccgccgcc aacggcttcc cgatcgacgc cgagaccggc
     6061 gagtatgatt acagcgcgct caaagtgcac gccgccgcgg cggcgggggt cgaggagggc
     6121 gatgtcgacc tgcgttggtg gctggaatgc gacggcgtcg agcaggacga atattatatg
     6181 cagtgccccg agggagaccc gtcggcgcgc tacctgcaag tccgggtcga ggcggcctat
     6241 acgccggtgt tcagcatatc ggcggccggc ctcctcggcg accagatcac catgtcggca
     6301 gaatcggcgg tacgcatcga atgaaacaga aaatcaacca ctttcgccgc gaccagaaag
     6361 gcgccggcgc catggaattc gcgctggccg cgccggtgct gttcacgatg atcgtcggca
     6421 tctcgcagat gggcaccttg ttcttcgcca atgcggggct cagccacgcg gtgtcggaag
     6481 gcgcgcgcat tgccgccaat ttcccgacgc ccgcggacga cgtgatcaag gcggcgatcc
     6541 tcgacaagag gttcggcctg gtcgaggaga atatcgtcgg cgagcccacg atcgtgcccg
     6601 gcaccgacac cgcgacgagc atgccgttcc tggacatcag cctcagctac gaggtgccct
     6661 tgaacttcat cttcttcacc gggcccgagg tgacgctcac cgagacccgc cgcgtcttca
     6721 ggcagatcga gactccggcg ccggtggatg aagacgacga ggatgaagac gagggcgagg
     6781 a
//
LOCUS       DASVRK010000232        14046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_597722, whole genome shotgun sequence.
ACCESSION   DASVRK010000232 DASVRK010000000
VERSION     DASVRK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597722"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VF589_09205"
     CDS             <1..1210
                     /locus_tag="VF589_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257800.1"
                     /translation="AYVVFGKAARSGNIDLGVLAAAQGFIVQGDADGDRAGRAVSTAG
                     DVNGDGFDDILVGAFAGDDGGPEAGEAYVVFGRAPVESVTRIGSFAGQTIRGGGGDDW
                     LDGRDGADRLFGAGGDDELIGGTGNDLLGGGSGIDSLRGGSGNDSLNGGGGGDTMNGG
                     SGNDRHYVDSAGDLVIEAAEGGTDRMFASVSYALADGAHVEMLTTIANSGTASIHLSG
                     NSGANRILGNNGGNILKGRSGNDDLNGLGGNDRIEGGEGKDLLAGGAGTDRFVFDTAP
                     GANDTDRISDFVAADDSILLNRLVYGAIAGDGMLAASQFHSGKAAADASDRIIHDADT
                     GIIFYDADGAGGAAQTAFARVTAGIALTRSDFIGFTSGGAMAATAPMAAEAGFTGFTA
                     MQAIGWADLP"
     gene            complement(1170..1805)
                     /locus_tag="VF589_09210"
     CDS             complement(1170..1805)
                     /locus_tag="VF589_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8257801.1"
                     /translation="MARARLSAFSSVFDPMRTSAVLRQPRSGCERRHVRRISEAAVEM
                     QQSIDLLLMLAREERARSPSAHVPLLPLVEKIVLAESARHDAGDFEVTVAVPADCTSR
                     VDARVAAAIIGNLIANVFQHTRPGRLGIAVEGTDLLISDSGEGIPAEMLQSISEDCAA
                     PKLRGLGLSIVSRLCRVHRIPLRIEPTPAGTRVAVGLLLREGPPSRSPASR"
     gene            complement(1787..1954)
                     /locus_tag="VF589_09215"
     CDS             complement(1787..1954)
                     /locus_tag="VF589_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257802.1"
                     /translation="MGDLKQWEKVQRLWDSGFRFGSLGQGGDGERFPDKLGEVDDFIR
                     RNPDHPWRVRG"
     gene            2814..4367
                     /locus_tag="VF589_09220"
     CDS             2814..4367
                     /locus_tag="VF589_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8257803.1"
                     /translation="MKRRRKLLRAAGLIGLAAAATAVGLAAAPLPRPEVRFSRPGWIG
                     PVAIVDVETGTVRRGKALRIERGRIAAVTDMAAVPPRDRPAMLNAGGAYVTPGLWDMH
                     AVLTRYAGALEHPLYLGYGVTRLRSILDCPAEGKASLYACGSHKRRWNRDVRAGRLIG
                     AITLGSGSFPVAGPRRLHRDLPDFYGAATPAEARRLVRFIAGQPAGPDHIKTYDGLTR
                     DSFFALMAEAKARGIEVSGHVPAAVRVSEAAAAGLKAVAHARALPIGCSTREGEIMRL
                     RSAARPAAQWMRLALQSQHPAACAALWRTLTRRGTFVSPTLITRYSETRAGLAELRGD
                     PATQRATPPLFEWIWSEDIAPVEARRPEEEAVYAAFYRAAARRTAEAEQAGVRLLAGS
                     DTGDAFVAPGVGLHQEMALWRRAGTPPAAILRAATIDAAAYFGQSHRIGRIAPGYVAD
                     LVFTGGNPLVDLGTLREPQAVLQEGRLYDRRALADARGAAEQAAASWRFPMHVLRDLM
                     RNPLGFTGS"
     gene            4476..4835
                     /locus_tag="VF589_09225"
     CDS             4476..4835
                     /locus_tag="VF589_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257804.1"
                     /translation="MNGPDWIKKAERVARTLGATTAGERRASHSVYVILLHDPSGIGR
                     WGLYVGQTSRDPDLRFDQHKAGHKSSRAVRRFGVRLLPHLVNHLNPMTGWESLELEAA
                     LAEGFRHAGIPWIEGGH"
     gene            complement(5078..7057)
                     /locus_tag="VF589_09230"
     CDS             complement(5078..7057)
                     /locus_tag="VF589_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257805.1"
                     /translation="MATLRGTAGGDTLSGTPENDFLDGLGGNDTLTGNNGDDTARGGI
                     GDDKLFGNLGNDILDGNVGNDTLDGGFGNDKLTGGDGADILIFNGGLDNLYGQAGDDI
                     IRIVGSGGGRVGISGGDGFDTFDARGATAGFFAQHAEGKTASEFELYYLAHFSGSWTG
                     SATAETFWCGGGNDTLFAGGGNDIVHGETGDDALVGDAGNDLLDGGTGFDLVYYNGYA
                     VSITASLTTNRGRGDTLGTDTFESIEGLVGSTVADTLTGSVSGDKLFGEAGNDRLLGV
                     DGNDELHGGEGNDTIFGGDGDDLMLGGLGTDTFDGGAGSDTVDYRHETTGVGIVLGGA
                     QTGAAAGDNLNNIDNVTGTNFADTLRGNNLANKLTGLAGENVLSGLGGVDVLTGGTSA
                     DTLDGGTGADTMMGSSGDDIYLVDSAEDVAFEYAFAGNDSVRSRAADYTLGANVELLA
                     LLGPALNGTGNETANGITGNTGANALTGLGGDDVIDGAGGADRIAGGAGNDVLTGGSG
                     RDIFVLDTAPDAAANVDDLADFRPIDDVIHLDRAVFGGIAADGALHGAAFKAGAAAGD
                     ASDRIIYNVGSGEIFYDADGTGGAAQVLIAKVDAGTPVTRADFVAYSAPAAAAAAPAA
                     AAWEAESDPTATMGLAGRDALLWRPEALGGDLQIA"
     gene            complement(7275..11138)
                     /locus_tag="VF589_09235"
     CDS             complement(7275..11138)
                     /locus_tag="VF589_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257806.1"
                     /translation="MATITGTSGNDVIDEAAEGGSAAAGSTINALAGDDRIIALRKTP
                     YSDGVGPIHITDTIDGGDGTDSYAVTFHNGWNISTAGHREHFSGGIGDWFAEFTSVER
                     FDVTAYLHGVAILGDGDDRVDVRLAGHSGTGNYGMGGGDDEALLSRGTANVNMGAGSD
                     RLVLDHAYANARTYMNVALSGDLASGYSGTYYRTDGAGVYAEISFSGVEHFTITTVAT
                     GSYTGDDIVTGNGNDVISTGAGDDFIRVARGADVVDGGDGVDALGIDLSDETAGVTIN
                     LLSSGAQQTGGTGSISGVESFVGTVTGSAQSDVLIEGVHHYNAQFSTGAGNDRVQVSR
                     GTDSVAMGSGFDRLVLDHSTANARTYMSVALSGDLASGYSGTYYRSDGAGVYAEVSFS
                     GVEHFTITTVASGGYTGDQIVTGNGNDIISTGAGDDLITVGRGADAVNGGAGVDALGI
                     DLSDESTGVTINLLLASGAQQTGGTGSVAAVESFLGAVAGSAHNDVLREGSHDFDSTF
                     ETGAGDDLVQVSNGDDVVNMGAGTDRLVVDYSYTNTHLNLGGSQPFSGTFAIGYAGEF
                     SLWGGAKTIFSGVEHFTVTTAGGLDYVVVGDGDDIVSTGAADDRVTAGRGVNQVDGGD
                     GTDALGIDFTGRASGVSLDLLLTGAQQAAGPGSVANFESFAGQVTGTEFDDLFREGVQ
                     AFSAVFETGAGNDLVQVSNGNDTVNMGAGTDRLVVDYSYTNTHLNLGGNPPFSGTLAN
                     GYAGEFSLWGGAKTIFSGVEHFTVTTAGGLDSVRVGDGDDIVSTGAADDWVTAGRGVN
                     QVDGGAGIDALSIDFTGRASGVSINLLAVGAQQAAGPGSVANFESFHGNVVGTRFNDV
                     FREGIHAFNATFDTGGGNDLVEVSRATDVVNMGGGSDRLVIDYSYVNGHFYGENQPFT
                     GTLAGGYSGQFYLGGAYAQTTFTGVEHFTITTAGQSDSVTVGDGNDIISTGLGADTIR
                     AAGGNDVLSGGEGNDILDGGAGADRMSGGLGDDSYAVDLGTDRATEAADAGIDTVTSQ
                     VDFRLGANVEHLILAGTARSGTGNGLDNNLTGNAAVNVLNGAGGADRLAGGAENDIYY
                     VDIAGDVIVEALGEGTDSVRSIGTYTLGDNVENLILTGLRVIGGTGNDLANGITGNAA
                     ANLLQGGGGDDRIVGGEGNDRLHGGLGGDQLSGGLGADSFGFDSALGAGNVDRLIDFS
                     ATDDTIRLDRAVFTGIGANGRLAASAFEEGTVALDAADRILYDAKSGSIFYDADGAGG
                     AAAILFARVAVNTALTEADFLAFMG"
     gene            complement(11280..13670)
                     /locus_tag="VF589_09240"
     CDS             complement(11280..13670)
                     /locus_tag="VF589_09240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8257807.1"
                     /translation="MPDPTLERDAVRLFEELLEIPEAERDAWLAKTAADRPELLSRLQ
                     SLREADRRAALRTGAGTEPLEGEQPPPERIGAYRIAERIGRGGMGSVYRAERATGDFA
                     HVVAIKIIKPGLLSEALVERFQRERQLLARLSHPNIARLYDGGEAEAGSPYIVMEYVD
                     GLPLLAWAEEQRLGRDERRRLFLDICAAVGFAHRHLIVHRDLTPSNVLVTREGVVKLI
                     DFGIARPADVEAEAGEAPRSRSIESLSLTPGFAAPERMTGAGVTTAADIYSLGRLLSR
                     LIPPGPQDRELAAIIAKATAADPANRYPTAEALSADVAAWQHGFPVRAYRTGRRYWLG
                     KFVRRHRLSVAAGAAALILLVAALGLTARAYAQAEAARRAEAARFADLRSLANYMVFD
                     LQGMLERVVGNAEARAALADRAQAYLSALAASPDADDGLKLEAARGLVALAYVQGVPA
                     QPNLGQADRARANLEQALKLLGGLQAAPAATAPPMAEALTARSMVEANIDADPKAAAK
                     TQQQAAALLERVPAEARGAPWHMARRRLRKAQTEVAVIAQEIDEIARLAGLLESDIKA
                     WPPAMQRAREAEFDRALAAHFRGYHGYFTDRLEASVQAMLDAERRLVALERTRPNDPL
                     VLFTLMWNGYVGYGAASGLPARAGEARRFLEVAERTSERLIQIEPNDHALKSFAGNVR
                     QIQSQALAAEGRHRQAIALQKQVIDLFEAALTPARRAAPLNRLTVAQLTLGNIAAGAG
                     DPALACSGYRAAQASIAELVRRDELLGFVESYREGVARNVGRCQRGAAAGDLEVLG"
     gene            complement(13663..>14046)
                     /locus_tag="VF589_09245"
     CDS             complement(13663..>14046)
                     /locus_tag="VF589_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX8257808.1"
                     /translation="VRLIQISQNNLSDRAHFIALASRMMRHILVDHARQKAAAKRRHV
                     KVEICTRIDGEQRLDLNRLDSALIRLGAIDEQLMELVEMRYFGGMTVADVSIVTGLSE
                     PTIKRRWQTARAWLTDALENPIDDA"
BASE COUNT         2127 a         4887 c         4792 g         2240 t
ORIGIN      
        1 ggcctacgtc gtgttcggca aggccgcccg ttcgggcaac atcgatcttg gcgtgcttgc
       61 cgcggcccaa ggcttcatcg tccagggcga cgcggacggc gaccgcgccg gccgggccgt
      121 gtccacggcc ggcgacgtca atggcgacgg cttcgacgac atcctcgtgg gtgcgttcgc
      181 cggcgacgat ggcggccccg aggccggcga ggcgtacgtc gtcttcggga gggcgccggt
      241 cgagagcgtc acccggatcg gctccttcgc cggccagacc atccgcggcg gcggcggcga
      301 cgattggctc gacggccgcg acggcgcgga tcgcctgttc ggcgccggcg gcgacgacga
      361 gctgatcggc ggcaccggca acgacctgct cggcggcggg tccggcatcg acagcctcag
      421 gggcggctcc gggaacgaca gcctcaacgg cggcggcggc ggcgatacga tgaacggcgg
      481 ctcgggcaac gaccgccact atgtcgacag cgcgggagac ctcgtcatcg aggccgccga
      541 gggaggcacc gaccgcatgt tcgccagcgt gagctacgcg ctcgccgatg gcgcccatgt
      601 cgagatgctg accaccatcg ccaattccgg cacggcgtcg atccacctct ccggcaattc
      661 gggcgccaac cggatattgg gcaacaatgg cggcaacatc ctgaagggcc ggagcggcaa
      721 tgacgatctg aacgggctcg gcggcaacga ccggatcgag ggcggggaag gaaaggattt
      781 gctggccggc ggagccggaa ccgaccgctt cgtgttcgac accgcgccgg gcgcgaacga
      841 taccgaccgc atcagtgact tcgtcgccgc cgacgattcg atattgctga atcggcttgt
      901 ctatggcgcc atcgccggcg acgggatgct ggccgcctcc caattccaca gcggcaaggc
      961 ggccgcggac gcgagcgacc ggatcatcca tgatgcggat accggcatca tcttctacga
     1021 tgccgacggg gccggcggag ccgcgcagac agcgttcgcc agggtgacgg cgggaatcgc
     1081 gctaacccga tccgacttca tcggcttcac cagcggcggc gccatggcgg cgaccgcgcc
     1141 gatggccgcc gaggcggggt tcaccggctt caccgcgatg caggcgatcg gctgggcgga
     1201 ccttccctga gcagcaatcc gaccgcgacc cgggtgcctg cgggcgtcgg ttcgatccgg
     1261 agcgggatgc ggtgtacgcg gcaaagccgc gacacgatcg acaggccgag gcctctcagc
     1321 ttcggagcgg cgcagtcctc cgaaatcgac tgaagcattt cggctgggat gccctcgccc
     1381 gagtccgaga tcaggagatc ggttccctcc accgcgatgc cgaggcggcc gggccgggtg
     1441 tgctggaaca cgttcgcgat gagattgccg atgatggccg cggcgacgcg tgcatcgacc
     1501 cggctggtgc agtcggccgg gacggcgacc gtcacctcga aatcgccggc atcatgccgc
     1561 gcgctctcgg ccaggacgat tttctcgacg agcggcagca gcggcacgtg cgccgaaggt
     1621 gatcgcgctc gttcctcgcg cgccagcatg agcagcaggt cgatcgactg ctgcatctcc
     1681 acggccgctt ccgagatgcg gcggacgtgg cggcgttcac atccacttcg cggttggcgc
     1741 aggacggccg atgtccgcat tgggtcgaag acggacgaga atgcgctcag ccgcgcacgc
     1801 gccatgggtg gtccgggttg cgcctgataa agtcgtcgac ctccccgagt ttgtcgggga
     1861 accgctcacc gtcgcctccc tggccaagac tgccgaaccg gaagccgctg tcccagagcc
     1921 gctgcacctt ctcccattgc ttgaggtcgc ccatcttcgg cgctttgaag gatcgcccca
     1981 tttcgctgag gcgccgcgcg cattgtgggc atagccggtc tttaccggcc ccatctttgc
     2041 cggccccatc cttccccgga gggagcttcc acgatttacg acagtgaaag caggcgtgcg
     2101 caatcctctg cctctgcggc ggctctggga cgggtcggcg attggccatc ctggaagatg
     2161 gcgaccttcg tgaatgtccg caatgggtcg aaagccgact caccgacctg ccgggtgaac
     2221 ctctccaagg atcaagaacc cacctcgcgg gaccgaatgg cagtagccgt ggataccgcc
     2281 ttcaaagcgt tcgcaatctg ataccaagcc aacagcggtg acttggcgcg gttccctgcg
     2341 ggcagcggca agtgtgctgt cgtcaccata gatgccaagc cggtgcccag ccactcgctc
     2401 gatccgatag gcacccggga tttctgcgtc ctcagccgat gcaaacaggg cacgcccggt
     2461 ccaccagcct cgaacagcaa cgcattcccc actgtgctgg cggtagttcc gagcaaggtc
     2521 ggtcaaagcc acgctagatg cccgtgcgag attgcagaaa gcaggatcgg ttgcgaggct
     2581 aacgatatcc ccctcaggct cgacaaggtc ggtttcaggg ccgctcccaa aagctccagc
     2641 taagaagaac caaactgcca acataggatc aatatcctca caacggctga ggtccgcaat
     2701 gggtcgctaa cggacattcg gcctttggtc agatttcaga ccgacaccct acccgctttc
     2761 acatcgcctt gacggcgggc aggctagcca ctcgctaaac cgggggagag agcgtgaaac
     2821 gaaggcgcaa attgctgcgg gcggcggggc tgatcgggct ggcggcggcc gcgaccgccg
     2881 tgggtctcgc ggccgcgccg cttcccaggc ccgaggtgcg cttcagccgc ccgggctgga
     2941 tcggcccggt cgccatcgtc gacgtggaga cgggcacggt ccggcgcggc aaggcgctcc
     3001 gcatcgagcg cggccgcatc gccgcggtga ccgacatggc cgccgtgccg ccgcgtgacc
     3061 ggcccgcgat gctcaacgcc ggcggcgctt atgtgacgcc cggcctgtgg gacatgcacg
     3121 cggtcctgac ccgctatgcc ggagcgctcg agcatccgct ctacctcggc tacggcgtca
     3181 cccgcctgcg ctcgatcctc gactgccccg ccgaaggcaa ggcgagcctc tacgcctgcg
     3241 gctcgcacaa gcggcgctgg aaccgggacg tccgggcggg ccggctgatc ggcgccatca
     3301 ccctgggctc gggcagcttc ccggttgccg ggccgcgccg gcttcaccgc gacctgcccg
     3361 atttctacgg cgccgccacc ccggccgagg cccggcggct ggtgcggttc atcgccgggc
     3421 agccggccgg ccccgaccac atcaaaacct atgacgggct aacgagagac tcgttcttcg
     3481 cgctgatggc cgaggcgaag gcgcgcggga tcgaggtcag cgggcatgtt cccgcggcgg
     3541 tccgggtctc cgaggccgcc gccgccgggc tcaaggcggt cgcccatgcg cgggcgctcc
     3601 cgatcgggtg ctcgacgcgg gaaggcgaga tcatgcgcct gcgctcggcc gcccggccgg
     3661 cggcgcaatg gatgcggctg gcgctccagt cccagcatcc agcggcctgc gcggcgcttt
     3721 ggcgaacact gacgcgccgc ggcactttcg tgtcgcccac cctcatcacg cgctattcgg
     3781 aaacgcgggc cgggcttgcc gagctcaggg gcgatccggc gacgcagcgg gccacgccgc
     3841 cgctgttcga atggatctgg agcgaggaca tcgcccccgt cgaagcgcgg cggccggagg
     3901 aggaggcggt ctacgccgcc ttctatcgcg cggccgcgcg ccgcaccgcc gaggccgagc
     3961 aagcgggcgt ccggctgctc gccggcagcg acaccggcga cgccttcgtc gcgcccggcg
     4021 tcgggctcca tcaggaaatg gcgctgtggc gaagggccgg caccccgccc gccgccatct
     4081 tgcgcgcggc cacgatcgat gccgccgcct atttcggaca gtcgcaccgc atcggccgga
     4141 tcgcgcccgg ctatgtggcc gatctggtgt tcaccggcgg caacccgctc gtcgaccttg
     4201 gaaccctgcg cgagccgcaa gcggtgttgc aggaggggcg gctgtacgac cgcagagcgc
     4261 tggcggacgc gcgcggcgcg gcggagcagg cggcggcgag ctggcgcttt cccatgcatg
     4321 tgctgcgcga tctgatgcgc aacccgctcg gcttcaccgg ctcatgatcc tggggatcgt
     4381 tcggcctgcc acgcagcggc gcggagggcc ccatcccagc ctccgcggtg gggctcgttg
     4441 cggccagtcg tccggcgctt taggttccgg tggagatgaa cggccccgac tggatcaaga
     4501 aagctgaacg ggtcgcccga actttaggcg ccaccactgc gggggagcgg cgcgccagcc
     4561 attcggtcta cgtcatcctg ctgcatgacc cgtccgggat cggaaggtgg ggcctgtatg
     4621 tcgggcagac ctcgcgcgac cccgatctgc gcttcgacca gcacaaggcc ggtcacaaat
     4681 cgagcagggc ggtcaggcgg ttcggagtga ggttgctgcc gcatctcgtc aaccacctca
     4741 acccgatgac aggctgggag tcgctcgaac tggaggcggc gctggcggag ggtttccgcc
     4801 acgccggcat cccttggatc gagggcggtc actgatgccg ccaggcgcct ggtgcggctg
     4861 cgaagagcag gcacctctcg cgacatcgag ccagccttcg gtcgggcagg cgctcacccg
     4921 atcgcagccg ctgcgctgaa gggtttcccg accgacctac tcccggggac ccgattctgg
     4981 gcgcgtcccc tcccggcagc gtggaaggca ttgcggcgcc ggattggaga gcaagccttg
     5041 ccatggccgc agcgcgatgc cgcggccccg ctcccggtca ggcgatctgc aggtcgccgc
     5101 caagcgcctc cggccgccac agcagcgcgt cgcgtccggc caggcccatc gtcgccgtcg
     5161 gatcgctctc ggcttcccag gcggccgccg cgggcgcggc agcggcggcg gccggcgccg
     5221 aataagcgac gaaatcggcg cgcgtcacgg gagttccagc atcgaccttg gcgatcagca
     5281 cctgggccgc gccgccggtg ccgtcggcat cgtagaatat ctcgccgctg ccgacattgt
     5341 agatgatccg gtcggaagcg tcgccggccg ccgcgccggc cttgaacgcg gccccatgaa
     5401 gcgcaccgtc ggcggcgatg ccgccgaaca ccgcgcggtc gagatggatg acgtcatcga
     5461 tggggcggaa gtcggcgaga tcgtcgacat tggcggccgc gtctggggcg gtgtcgagca
     5521 cgaatatgtc gcggccgctg ccgccggtca ggacatcgtt gccggcccca ccggcgatcc
     5581 tgtcggcgcc gcccgcgccg tcgatgacat cgtcgccgcc gaggccggtc agcgcgttcg
     5641 cacccgtgtt tccggtgatc ccgttggcgg tctcgttgcc ggtcccgttg agcgccggtc
     5701 cgagcagcgc cagcaactcg acgttcgcgc cgagcgtgta atccgccgcc cgcgagcgca
     5761 cgctgtcatt gcccgcgaac gcgtattcga aggccacgtc ttcggcgctg tcgaccagat
     5821 agatgtcgtc gccactggag cccatcatgg tatcggcgcc ggtgccgccg tcgagcgtgt
     5881 cggccgaggt cccgccggtc agcacgtcga cgccgccgag cccgctgagg acgttctcgc
     5941 cggcgaggcc ggtcagcttg ttcgccagat tgttgccgcg caaagtgtcg gcgaagttgg
     6001 tgccggtgac attgtcgata ttgttgagat tgtcgccggc cgcggcgccg gtctgggcgc
     6061 cgccgagcac aatgccgacg cccgtcgttt catggcggta atcgaccgtg tccgagcccg
     6121 cgccgccgtc gaacgtgtcg gttccaaggc ccccgagcat cagatcgtcg ccgtcgccgc
     6181 cgaagatcgt gtcgttgccc tcgccgccgt ggagctcgtc attgccgtcc acgcccaaca
     6241 ggcggtcgtt gccggcctcg ccgaacaatt tgtcccccga gaccgagccg gtcagcgtat
     6301 cggcgacggt gctgccgacc aggccctcaa tgctttcgaa cgtgtcggtg ccgagcgtat
     6361 cgccgcgccc gcgattggtg gtgaggctgg cggtgatcga aaccgcgtag ccgttataat
     6421 aaaccaggtc gaagccggtg ccgccgtcga gcaggtcgtt gccggcatcg ccgaccagcg
     6481 catcgtcgcc ggtctcgccg tggacgatgt cgttgccgcc gccggcgaaa agggtgtcgt
     6541 tgcccccgcc gcaccagaag gtctcggccg tggccgagcc ggtccacgag ccgctgaaat
     6601 gggcgagata ataaagctcg aattcgctcg cggttttgcc ctcggcatgc tgggcgaaga
     6661 agccggcggt cgcgccgcgc gcgtcgaacg tgtcgaaacc gtcgccgccc gaaatcccga
     6721 cccggccgcc gccgctgccg acgatcctga tgatgtcgtc gccggcctgg ccgtagaggt
     6781 tgtcgagccc gccgttgaag atcaatatat ccgcgccgtc gccgccggtg agcttgtcgt
     6841 tgccgaagcc gccgtcgagc gtgtcgttgc cgacattgcc gtcgagaatg tcgttgccga
     6901 gattgccgaa cagcttgtcg tcgccgatgc cgccgcgcgc ggtgtcgtcg ccattgttgc
     6961 cggtcagcgt gtcgttgccg ccgagcccgt cgaggaaatc gttctcgggt gtgccgctca
     7021 gcgtgtcccc gcccgcagtg cctctcaaag tcgccatgat gtctctcccc ctcataagaa
     7081 caggcgtcga aaaccgacgc ttcggatcag tgggagagag atttattttg cagcaggcaa
     7141 agcggacatt cggatgggcg gacgcggtcg cttcgcgcgg acgcctccgc cgtcattccc
     7201 gcgaacgcgg gatcccgctt cccttcctcc aggccggaag cgggactccc gctttcgcgg
     7261 gagtgacgaa ccgatcaccc catgaaggcg aggaagtccg cctcggtcag cgcggtgttg
     7321 acggcgaccc gcgcgaacag gatcgcggcc gccccgcccg ccccgtccgc atcgtagaag
     7381 atcgagccgg acttggcgtc gtagaggatg cggtccgcgg catcgagcgc gaccgtgccc
     7441 tcctcgaacg ccgaagccgc cagccggccg ttcgccccaa tcccggtgaa caccgcccgg
     7501 tcgagccgga tcgtgtcgtc ggtggccgag aaatcgatca gccggtcgac attgcccgcg
     7561 ccgagcgcgc tgtcgaagcc gaagctgtcg gcgccgaggc cgccgctcaa ttggtcgcca
     7621 ccgaggccgc cgtgcagccg gtcattgccc tcgccgccga caatgcgatc gtcgccgccg
     7681 ccgccctgca gcaaattggc ggccgcgttg ccggtgatgc cgttggcgag atcgttgccg
     7741 gtgccgccga tcacgcgcag gccggtcagg atcagattct cgacattgtc gccgagcgtg
     7801 taggtcccga tgctgcggac gctgtcggtc ccctcgccca aagcctcgac gatcacgtcg
     7861 ccagcgatat cgacataata aatatcgttc tccgctccgc ccgcgaggcg gtcggcgccg
     7921 ccagcgccgt tcagcacgtt gaccgccgca ttgccggtaa gattgttgtc gagcccgttg
     7981 ccggtgccgc tccgcgcggt tcccgcgagg atcagatgct cgacgttcgc gccgagccgg
     8041 aaatcgacct ggctggtgac ggtgtcgatg cccgcatcgg ccgcctcggt cgcgcggtcg
     8101 gtgccgagat cgacggcgta gctgtcgtcg ccaaggccgc ccgacatacg gtcggcgccg
     8161 gcgccgccgt cgagtatgtc gttgccctcg ccgcccgaaa gaacgtcgtt tcctccggcc
     8221 gccctgatcg tgtcggcacc gagaccggtc gaaatgatgt cgttgccgtc gccgacggtg
     8281 acgctgtcgc tctggccggc ggtggtgatg gtgaaatgct cgaccccggt gaaggtggtc
     8341 tgcgcgtagg cgccgccaag gtagaactgg cccgaatagc ccccggccag cgtccccgtg
     8401 aacggctggt tctcgccgta gaaatggccg ttgacgtagc tatagtcgat caccagccgg
     8461 tcgctgccgc cgcccatgtt gacgacgtcg gtggcgcgcg acacctccac caggtcgttg
     8521 ccgccgccgg tgtcgaaggt ggcgttgaag gcgtggatgc cttcgcggaa cacatcgttg
     8581 aagcgggtcc cgaccacatt gccgtggaag ctttcgaagt tcgccaccga acccggcccc
     8641 gccgcctgct gcgcaccgac ggcgagcaga ttgatcgaaa ccccgctggc gcggccggtg
     8701 aagtcgatgc tgagcgcatc gatcccggcg ccgccatcaa cctggttgac gcccctgccg
     8761 gcggtgaccc aatcgtcggc cgcaccggtc gagacgatgt cgtcgccgtc gccgacgcgg
     8821 acgctgtcga ggccgccggc cgtcgtgacg gtgaaatgct cgacgccgct gaagatggtc
     8881 ttggcgccgc cccaaagcga gaattcgccc gcatagccgt tggccaaggt gccgctgaac
     8941 ggcgggttgc cgccgaggtt caaatgggtg ttggtgtagc tataatcgac caccagccgg
     9001 tcggtaccgg cgcccatgtt cacggtgtcg ttgccgttgg agacctgcac cagatcgttg
     9061 ccggcgccgg tctcgaagac cgcgctgaag gcctgaacgc cttcgcggaa caggtcgtca
     9121 aactcggtgc cggtcacctg gccggcaaag ctttcgaagt tcgccacgct gcccggcccc
     9181 gccgcctgct gcgcgccggt gagcaggagg tccagtgaaa cgccgctcgc ccggccggtg
     9241 aagtcgatgc cgagcgcgtc ggtcccgtcg ccgccatcga cctggttgac gcctctgccg
     9301 gcggtgaccc gatcgtcggc cgcaccggtc gagacgatgt cgtcgccgtc gccgacgacg
     9361 acatagtcga ggccgccggc cgtcgtgacg gtgaaatgct cgaccccgct gaaaatggtc
     9421 ttggcgccgc cccaaagcga gaattcgccc gcatagccaa tggcgaaggt gccgctgaaa
     9481 ggctggctgc cgccgaggtt caggtgggtg ttggtgtagc tatagtcgac caccagccgg
     9541 tcggtgccgg cgcccatgtt gacgacatcg tcgccattcg aaacctgcac cagatcgtcg
     9601 ccggcgccgg tctcgaaggt ggagtcgaag tcgtgactgc cttcgcgcag cacgtcattg
     9661 tgggcggatc cagccaccgc gccgaggaag ctctccacag cggcgacgga gccggtgccg
     9721 ccggtctgct gcgcccccga cgcgagcaac aagttgatcg tgacgcctgt gctctcgtcg
     9781 gacaggtcga tgccgagcgc gtcgacgccg gcgccgccat tgaccgcgtc ggcgccgcgc
     9841 ccgaccgtga tcaaatcgtc gcccgcgccg gtcgagatga tgtcgttgcc gttgccggtg
     9901 acgatctggt cgcccgtata gccgccgctg gccacggtcg tgatcgtgaa atgctcgacg
     9961 ccgctgaagc tgacctcggc atagaccccc gcgccgtcgc tgcggtaata ggtgcccgaa
    10021 tagccggacg cgaggtcgcc cgacagcgcc acgctcatat aggtgcgggc gttggcggtg
    10081 gaatggtcga gcaccagccg gtcgaagccg gaccccatcg cgacgctgtc ggtgccgcgc
    10141 gacacctgca cgcggtcgtt gccggcgccg gtcgagaatt gcgcattata gtggtggacg
    10201 ccctcgatca ggacatcgct ctgggcggag ccggtcacgg tcccgacgaa gctctcgacg
    10261 ccgctgatgg agccggtccc gccggtctgc tgcgcccctg agctgagcag gttgatcgtg
    10321 acgccggcgg tctcgtcgga caggtcgatg ccgagcgcgt cgacgccgtc gccgccgtcc
    10381 accacgtcgg cgccgcgcgc cacccttatg aaatcgtcgc ccgcgccggt cgagatgacg
    10441 tcgttgccgt taccggtgac gatgtcgtcc cccgtatagc tgccggtggc caccgtcgtg
    10501 atcgtgaaat gctcgacgcc gctgaagctg atctcggcat aaacccccgc gccgtcggtg
    10561 cggtagtagg tgcccgaata gccggacgcg aggtcgcccg agagcgcgac gttcatatag
    10621 gtgcgggcgt tggcataagc atggtcgagc accagccggt cgctaccggc gcccatgttc
    10681 acattggccg tgccacggct cagcagcgcc tcgtcgtcgc cgccgcccat cccgtaattg
    10741 cccgtgcccg aatggccggc gaggcgaacg tcgacccggt cgtcgccgtc gcccaggatc
    10801 gcaacgccgt ggaggtaggc ggtgacgtcg aaccgctcga ccgaggtgaa ctcggcgaac
    10861 cagtcgccga ttccgccgct gaaatgctcg cgatgtccgg cggtcgagat attccagcca
    10921 ttgtggaacg tcaccgcata gctgtcggta ccgtcgccgc cgtcgatcgt gtcggtgatg
    10981 tggatcggcc cgacaccgtc ggaatagggc gtcttgcgaa gcgcgatgat gcggtcgtcg
    11041 ccggccagcg cattgatggt gctgccggct gccgccgagc ccccttcggc agcctcgtca
    11101 atcacgtcat tgccgctggt cccggtgatg gtcgccatgt cgtcgccccc ttatcccctg
    11161 ccgccccgca tgacggctta cctggatatg cgtgggatcg gcggaaacgg gatcatcggc
    11221 cggaagattt atttcgattg agggacttgg atcggccggg ccggttgatt tcgctagggt
    11281 cagcccagca cctcgagatc gcccgcggcc gcgccgcgct ggcagcggcc gacgttccgg
    11341 gcgacacctt cgcggtaact ttcgacgaag ccgagcagct cgtcccgccg cacgagctcc
    11401 gcgatcgagg cctgcgcggc gcgatagccg gagcaggcga gcgccgggtc gcccgccccg
    11461 gccgcgatgt tgccgagcgt caattgcgcc accgtcagcc ggttgagcgg cgctgcccgg
    11521 cgggccggcg tcagcgccgc ttcgaacagg tcgatcactt gcttctggag cgcgatggcc
    11581 tggcggtggc gtccctcggc ggcgagcgcc tgcgactgga tctgccgcac attgccggcg
    11641 aacgacttca gcgcatggtc gttgggctcg atctggataa gccgctcgct ggtccgctcg
    11701 gccacctcga ggaagcggcg cgcctcgccc gcccgcgccg gcaggcccga ggccgcgcca
    11761 tagccgacat agccgttcca catcaaagtg aagagcacga gcggatcgtt ggggcgcgtc
    11821 cgctcgagcg cgaccagccg ccgctcggcg tcgagcatcg cctgcacgct ggcttcgagc
    11881 cggtcggtga aatagccgtg atagccgcga aaatgcgccg ccagcgcgcg gtcgaattca
    11941 gcctcgcggg cgcgctgcat cgccggcggc catgccttga tgtcgctctc gagcaggccc
    12001 gcgagccgtg cgatctcgtc aatctcctgc gcgatcaccg ccacttcggt ctgcgccttg
    12061 cgcaggcgcc ggcgggccat gtgccagggc gccccgcgcg cctccgccgg cacccgctcg
    12121 agcaaagccg ccgcctgctg ctgggtcttg gcggcggcct tggggtcggc gtcgatattc
    12181 gcctccacca tcgagcgggc cgtcaacgct tccgccatcg gcggcgcggt cgcggccggc
    12241 gcggcctgaa gcccgccgag cagcttcagc gcctgctcca gattggcgcg ggcgcggtcg
    12301 gcctggccga ggttgggttg cgccggcacg ccctgcacat aagcgagcgc gaccagcccc
    12361 cgtgccgcct cgagcttcag cccgtcatcg gcgtccggcg aggcggcgag cgcggacaga
    12421 taggcctgtg cccggtccgc cagcgccgcc cgcgcctcgg cattgccgac cacccgctcc
    12481 agcatgccct gaaggtcgaa caccatataa ttggcgaggc tgcgcaggtc cgcgaaccgc
    12541 gccgcctccg cgcgccgcgc cgcttccgcc tgggcgtagg cgcgggcggt gaggccgagc
    12601 gcggcgacga gcaggatcag ggccgccgcg cccgccgcaa cggaaagccg gtgccgccgg
    12661 acgaacttgc ccagccaata gcgtcttccg gtccgatagg cgcgcaccgg aaagccgtgc
    12721 tgccacgccg cgacgtcggc actgagcgcc tcggcggtcg gatagcggtt ggccggatcc
    12781 gccgccgtcg ccttggcgat gatcgccgcg agctcgcggt cctgcggccc cggcgggatc
    12841 agccggctaa gcagccgccc gagcgaatag atgtccgccg ccgtcgtcac gccggcgccg
    12901 gtcatccgct cgggcgccgc gaagcccggc gtaaggctga ggctttcgat cgagcgcgag
    12961 cgcggcgcct cgcccgcctc cgcctcgacg tcggcgggcc gggcgatgcc gaaatcgatc
    13021 agcttcacca ccccctcgcg cgtcaccagc acgttcgagg gcgtgaggtc gcggtggacg
    13081 atcaggtggc ggtgcgcgaa gccgactgcg gcgcagatgt cgaggaacag ccgccgccgt
    13141 tcgtcgcgtc cgagccgctg ctcctccgcc caggcgagca gcggcaggcc gtcgacatat
    13201 tccatgacga tatagggcga tccggcctcg gcctcgccgc catcgtagag ccgggcgatg
    13261 ttcgggtgcg acagccgcgc cagcaattgc cgctcgcgct ggaagcgctc gaccaacgcc
    13321 tcggagagca ggcccggctt gatgatcttg atcgcgacca cgtgcgcgaa gtcgccggtc
    13381 gcccgttcgg cgcggtagac gcttcccatg ccgccgcggc cgatccgctc ggcgatccgg
    13441 tacgccccga tccgctcggg cggcggctgc tcgccctcga gcggctcggt gccggcgccg
    13501 gtgcgaagcg ccgcccgccg gtcggcctcg cgcagcgact gcagccggct cagcagctcc
    13561 ggccggtcgg cggccgtctt ggccagccag gcgtcgcgct cggcttcggg tatttcgagc
    13621 agctcctcga acagccggac agcgtcgcgc tcgagcgtgg ggtcaggcat cgtcgatcgg
    13681 attttcgagc gcgtcggtga gccaggcgcg cgccgtctgc cagcgccgtt tgatggtcgg
    13741 ctccgacagc ccggtcacga tgctcacgtc cgccacggtc atgcccccga aatagcgcat
    13801 ctccaccagc tccatgagct gctcgtcgat ggctcccaat ctgataagcg cggaatcgag
    13861 gcggttgaga tcaagccttt gctcgccgtc gatccgggtg cagatctcga ccttgacgtg
    13921 gcggcgcttg gccgccgcct tctggcgggc atgatcgacc aggatgtggc gcatcatgcg
    13981 cgaggcgagc gcgatgaaat gagcgcggtc cgataaattg ttctggctga tctggatgag
    14041 ccgcac
//
LOCUS       DASVRK010000233         6926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_544315, whole genome shotgun sequence.
ACCESSION   DASVRK010000233 DASVRK010000000
VERSION     DASVRK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544315"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VF589_09250"
     CDS             <1..916
                     /locus_tag="VF589_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8257809.1"
                     /translation="APGADLVTIPSLRGAVVAVGDRRVSDMEDIPEGAWFLHGDRGLT
                     YASAVPEGSRIVEGRWWPADYRGPPLISLDVEAARAIGLKVGDRLTVAILGREIEARI
                     ASLREINWDTMGFNFVIIFSPGTLEAAPHSFMATAAMPEAQERPFARAVANAFPSVSA
                     IRVKEVVETVAGMLGQLSVAVRAAASVAILAGIAVLIGALAASRRARVYDSVMLKLLG
                     ATRARILAAQALEFAALALVVSAVAFALGAASGWYVVVKVLELDWAPDWGIVLATLAA
                     GALVTLAIGLLGSLPALAARPARALRTL"
     gene            958..1617
                     /locus_tag="VF589_09255"
     CDS             958..1617
                     /locus_tag="VF589_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8257810.1"
                     /translation="MIDLYTAATPNGWKVSITLEELELPYRLHVVDLAAGEQKQRPFT
                     DINPNGRIPAIVDRANGDFPIFESGAIMLYLAERAGRLLPADEKGRSRAVQWLMFQMG
                     GVGPMMGQANVFTRYFPEPLPSVIDRYRREGRRLFDVLDGHLASHEYLAGDYSIADIA
                     NWSWVHTHEWPGIDITGLEHLKRWLDAIAARPAVQKGVATPPRTRDADQTVATARKML
                     A"
     gene            1648..3168
                     /locus_tag="VF589_09260"
     CDS             1648..3168
                     /locus_tag="VF589_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8257811.1"
                     /translation="MNEMTAIAAQPAYSEGVQQRLRRAPALFIDGEWVASSHDKTLAV
                     DDPSTGREISAIADASDADVDRAVAAARRAFDDGRWSGLAPYRRERLVSRLADLIEEN
                     IAELAELESIDNGKPRSASQGYDIPRAVATLRYMAGWATKLSGEYIEPATMPSGAVHA
                     YVRREPIGVCAQIVPWNFPLMMAVQKIAPALAAGCTLVLKPAEQTSLTALRLADLVAE
                     AGFPAGVFNLVTGLGETAGDRMVRSPLVDKVAFTGSTQVGKLINIAATDTLKRVTLEL
                     GGKSPVIVLPDVDIARTAAGAARSIFANAGQVCIAGSRLFAHRSIFDELLEAVAANAG
                     EWKVGPSLAPDTAMGPLVSTEQRDRVMDYIDQGRKAGASVLSGGDAPSGPAYGDGYYV
                     NPTILVDVKPEMSVVREEIFGPVLTAQRFDDLDAVAAAANDTEYGLAASIWTRDVSAM
                     HKLAARIKAGMVWGNTNSAADTALPFGGFKQSGFGRESGRHGIEAYTELKTVAIAL"
     gene            3170..4018
                     /locus_tag="VF589_09265"
     CDS             3170..4018
                     /locus_tag="VF589_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257812.1"
                     /translation="MLRALLALALAAVVALPAAAQSPAAQSPAAPTPAERAQLDRVLE
                     RGRLIHAIDRAAWVVTDDFMAKAPAAVKAAARGYVVEPSAEGFAVTFYDDVSGRRSAL
                     YVGRVAGGRVVGSEVIAAARRAPLTPAQTRLVKAREAAATADYQSCTGAFNVTAIPPA
                     FPAAPLDVYLLSAQVNDREYPAGGHFRLVLAPDGKLVSSRKFTNSCLSLSGAPDSGGP
                     VAALMVTHLLDPLPTEIHVFTALSAGKPLYVGAGDRVWSVTGEAITLIGNIGEEMKDA
                     PATDPR"
     gene            complement(4197..4439)
                     /locus_tag="VF589_09270"
     CDS             complement(4197..4439)
                     /locus_tag="VF589_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX8257813.1"
                     /translation="MLTYALRLADRYDGMVMATCPDVPEVLALGRDSEEARDEAVRAL
                     EAALEGYEAEGREFPPPRACGTCSVTTTRFGELAPA"
     gene            complement(4727..4990)
                     /locus_tag="VF589_09275"
     CDS             complement(4727..4990)
                     /locus_tag="VF589_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257814.1"
                     /translation="MRVLALCALPLLSGCLSAAVDLVTLPVKAASTAVGAAGKAVDLA
                     TTSQAEADQKRGRAVRLREQCIGEEQRRAARANRRPDYGRCKG"
     gene            5180..5350
                     /locus_tag="VF589_09280"
     CDS             5180..5350
                     /locus_tag="VF589_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257815.1"
                     /translation="MNMSDLEYYRSRIAAEEIAAQRATHPLAAESHKRLAEEYASLIV
                     ATEGDAMRTATG"
     gene            complement(5564..6283)
                     /locus_tag="VF589_09285"
     CDS             complement(5564..6283)
                     /locus_tag="VF589_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8257816.1"
                     /translation="MSPLILRLETYVPLPEQDRASIDLLATRSVREVPARRDLLREGE
                     LPRALILILEGWACRYKALPDGRRQIVAFLVPGDLCNHHFALQRAMDHSIGAITPIKV
                     AELPRDEFARLAGERAPIAEGLTWDQLVEVAVQREWTLNVGQRTAFERISHLLCEMFL
                     RLRAVGLTSGDSCDFPLTQADIADAAGLTAVHVNRTLQDLRREGLIELQNRTLTIPDL
                     DALMAAGMFNPNYLNLARRAG"
     gene            6805..>6926
                     /locus_tag="VF589_09290"
     CDS             6805..>6926
                     /locus_tag="VF589_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8257817.1"
                     /translation="MFARTDRLLLRPGWAEDAPALYAAIADEAIVRNLASAPWP"
BASE COUNT         1086 a         2614 c         2132 g         1094 t
ORIGIN      
        1 cgcgcccggc gccgacctcg tcaccatccc ctcgctgcgc ggcgcggtgg tcgcggtcgg
       61 cgatcgccgc gtctccgaca tggaggatat tccggaaggc gcctggttcc tccacggcga
      121 ccgcggcctc acttatgcga gcgcggtgcc cgaagggagc cggatcgtcg aaggccgctg
      181 gtggccggcc gattatcgcg ggccgccgct catctcgctc gacgtcgagg ccgcccgcgc
      241 gatcgggctc aaggtcggcg accgcctcac cgtcgccatc ctcggccgcg agatcgaggc
      301 ccggatcgcc tccctgcgcg agatcaactg ggacacgatg ggcttcaact tcgtcatcat
      361 cttcagcccc ggcacgctcg aagcggcgcc gcacagcttc atggccaccg ccgccatgcc
      421 cgaagcgcag gagcggccgt tcgcgcgcgc cgtcgccaac gccttcccga gcgtcagcgc
      481 gatccgggtc aaggaggtgg tcgagaccgt cgccggcatg ctcggccaat tgtcggtcgc
      541 ggtccgcgcc gccgcctcgg tcgcgatcct cgccggcatc gccgtgctga tcggcgcgct
      601 cgccgccagc cgccgcgccc gcgtctatga cagcgtcatg ctgaaattgc tcggcgccac
      661 ccgcgcccgc atcctcgccg cccaggcgct cgaattcgcc gcgctcgcgc tcgtggtcag
      721 cgcggttgcc ttcgcgctcg gcgccgcgtc gggctggtat gtcgtggtga aggtgctgga
      781 gctcgattgg gcgccggact ggggcatcgt cctcgccact ttggccgccg gcgccttggt
      841 caccctcgcc atcggcctcc tcggctcgct ccccgcgctc gccgcccgcc cggcccgggc
      901 attgcggacg ctctaggccg gtcctatttc gcaaccttcg aaccgggagc cccgccgatg
      961 atcgacctct acaccgccgc cacgcccaat ggctggaagg tgtcgatcac gctcgaggag
     1021 ctcgaactgc cctaccgcct ccacgtcgtc gacctcgccg ccggcgagca gaagcaaagg
     1081 ccgttcaccg acatcaaccc caatggccgc atccccgcca tcgtcgaccg cgccaatggc
     1141 gatttcccga tcttcgaatc gggcgcgatc atgctctatc tcgccgagcg cgccggccgg
     1201 ctgctccccg ccgacgagaa gggccgcagc cgcgccgtcc agtggctgat gttccagatg
     1261 ggcggcgtcg gcccgatgat gggccaggcc aacgtcttca cccgctattt ccccgagcca
     1321 ttgccgagcg tgatcgaccg ttaccggcgc gagggccggc ggctgttcga cgtgctcgac
     1381 ggccacctcg cctcccacga atatctcgcc ggcgactatt cgatcgccga catcgccaac
     1441 tggtcctggg tccacaccca cgaatggcca ggcatcgaca tcaccggcct cgagcacctc
     1501 aaacgctggc tggacgcgat cgccgcccgc cccgccgtcc agaaaggcgt cgccacccca
     1561 ccccgcaccc gcgacgccga ccagaccgtc gcaaccgccc gcaagatgct ggcctgattt
     1621 tcctccccgc gatttgaagg aagccccatg aacgagatga ccgccatcgc cgcccagccc
     1681 gcttattccg aaggcgtcca gcagcggctg cggcgcgcgc cggctctgtt catcgacggc
     1741 gaatgggtcg cctcgagcca cgacaagacg ctggccgtcg acgatccctc caccggccgc
     1801 gaaatatccg ccatcgccga cgccagcgat gccgatgtcg accgcgccgt cgccgccgcc
     1861 cgccgcgcct tcgacgacgg ccgctggtcc ggcctcgccc cctatcgccg cgagcgcctg
     1921 gtcagccgcc tcgccgacct catcgaagag aatatcgccg agctcgccga actcgaatcg
     1981 atcgacaacg gcaagccgcg ctcggccagc cagggctacg acatcccgcg cgcggtcgcg
     2041 actttgcgct acatggccgg ctgggcgacc aagctcagcg gcgaatatat cgagcccgcc
     2101 acaatgccct cgggcgccgt ccacgcttat gtccggcgcg agccgatcgg cgtctgcgcc
     2161 cagatcgtgc cgtggaactt cccgctgatg atggcggtgc agaagatcgc gcccgcactc
     2221 gccgccggct gcacattggt cctgaagccc gccgagcaga ccagcctcac cgcgctccgc
     2281 ctcgccgatc tcgtcgccga agccggcttc ccggccggcg tgttcaacct cgtcaccggc
     2341 ctcggcgaga ccgcgggcga ccgcatggtc cgctcgcctc tggtcgacaa ggtcgctttc
     2401 accggctcga cccaggtcgg caagctcatc aacatcgccg ccaccgacac gctgaagcgg
     2461 gtgacgctcg agctcggcgg caagtccccg gtcatcgtcc ttcccgacgt cgacatcgcc
     2521 aggaccgcgg ccggcgccgc ccgctcgatc ttcgccaatg ccggccaggt gtgcatcgcc
     2581 ggctcgcgcc tgttcgccca ccgctcgatc ttcgacgaat tgctggaggc cgtcgccgcc
     2641 aatgccggcg agtggaaggt cggccccagc ctcgcccccg acacggcgat gggccctttg
     2701 gtctcgaccg agcagcgcga ccgggtgatg gactatatcg accagggccg caaagccggc
     2761 gccagcgtcc tcagcggcgg cgacgcgccg tccggccccg cttatggcga cggctattac
     2821 gtcaacccca ccatcctcgt cgacgtgaag ccggaaatgt cggtggtccg cgaggagatt
     2881 ttcggtccgg tgctcaccgc ccagcgcttc gacgatctcg acgccgtggc ggcggccgcc
     2941 aacgacaccg aatatggcct cgccgcctcg atctggaccc gcgacgtctc ggcgatgcac
     3001 aagctcgccg cccggatcaa agccggcatg gtctggggca acaccaattc ggccgccgac
     3061 accgcgctcc ccttcggcgg cttcaagcaa tcgggcttcg gccgcgaatc gggccgccac
     3121 ggcatcgagg cctataccga actcaagacc gtcgcgatcg cgctctgaca tgctgcgcgc
     3181 gctcctcgcc ttggcgttag cggcggtcgt cgcgcttccg gccgccgccc aaagccccgc
     3241 cgcccaaagc cccgccgccc caacccccgc tgaacgcgcc cagctggacc gcgtgctgga
     3301 gcgcggccgg ctcatccacg ccatcgaccg cgcggcctgg gtcgtcaccg acgatttcat
     3361 ggccaaagcc ccggccgccg tcaaagcggc cgcgcgcggc tatgtggtcg agccgtcggc
     3421 ggagggcttc gccgtcactt tctacgacga tgtcagcggg cgccgttcgg ccctctatgt
     3481 cggccgggtc gccggcggcc gcgtcgtcgg cagcgaagtc atcgccgccg cccgccgcgc
     3541 gccgctcacc cccgcgcaga cccgcctggt gaaggcgcgc gaggccgcgg ccacggccga
     3601 ctaccaatcc tgcaccggcg cgttcaacgt caccgccatc ccgcccgcct tccccgcggc
     3661 gccgctcgac gtctatctgc tgagcgcgca ggtgaacgac cgcgaatatc cggccggggg
     3721 ccatttccgg ctcgtgctcg cgcccgacgg caagctcgtc tcctcgcgca agttcaccaa
     3781 ttcctgcctc agcctgtccg gggcgcccga ttccggcggc ccggtcgccg cgctgatggt
     3841 cacccacctg ctcgatccgc tgccgaccga gatccacgtc ttcaccgcgc tcagcgccgg
     3901 caagccgctc tatgtcggtg ccggcgaccg ggtgtggagc gtcaccggcg aggcgatcac
     3961 cctgatcggc aatatcggcg aggaaatgaa ggacgccccc gccacggacc cgcgctgacc
     4021 tggcggccgc ccgttcgttc gcccatcaag gcacgcaccg tcataccagc gaaagctggg
     4081 atctcccttt tcttggaccc gcttaaggta agagagattc cagctttcgc tggaatgacg
     4141 gtctagattt gctggattga gggccgtgcc ggacgctcgt ccgccgccgc cgcaggtcag
     4201 gcgggcgcca gctccccgaa ccgtgtcgtg gtgacgctgc acgtgccgca ggcccgcggc
     4261 ggcgggaatt cgcggccctc ggcctcataa ccttccagcg ccgcttccag cgcccgcacc
     4321 gcctcgtccc gcgcctcttc ggagtcgcgg ccgagcgcga gcacttcggg cacgtccggg
     4381 caggtggcca tcaccatccc gtcatagcgg tccgcaagcc tcagcgcata agtcagcatg
     4441 ccggtctcct tcaggttacc aggttccacg cgctccgaaa tttcggacca ccggcgcggc
     4501 acgcatattc aatgtcgggt gccgccagct tacgatgcgg cctttccctg tctttgatga
     4561 tgaggaagat gatgcggatg gcgttcgcgc gcgagtcccc gaatcggcaa ctcggctcac
     4621 gccggagccg tttcggcgcg gaaattaacc gctccgggcg tgatcgtccg gcctggaacc
     4681 cgcgcgcctt gccgcgcggc ccgtgaatca gctccgcctc aagcctctag cccttgcacc
     4741 ggccgtaatc gggccgccga ttcgcgcgcg ccgcccgcct ttgctcctcg ccgatacatt
     4801 gttcgcgcag ccgcaccgcg cggccccttt tctggtcggc ttcggcctgg ctggtggtgg
     4861 cgaggtcgac cgctttgccg gccgccccga ccgcggtcga cgccgccttc accggcagcg
     4921 tcaccaggtc caccgccgcg ctgaggcagc ccgacagcag cggcaacgca cataaagcca
     4981 gaacccgcat cgccatctcc ctggaagccc gctcacccta atcgccgacc gaagctgatc
     5041 gccaactgaa cctgccgccg gctccccttt ccccgcgccc cgttgccctg aatcaacagc
     5101 tgctgaccca tgttagtggc ggggtaatcc ccgatccgct attattggcc ctcagccgag
     5161 tcccgggggg gtcacttcga tgaacatgtc cgatctggaa tattatcgct cacgtatcgc
     5221 cgccgaggag atcgccgccc agcgcgcgac ccatccgctc gcggccgaaa gccacaagcg
     5281 cctcgccgag gaatatgcca gcctgatcgt cgccaccgaa ggcgacgcga tgcgcaccgc
     5341 caccggctga tcggggcggg cgggccggtg gcctcccgtc attccagcga aactactccg
     5401 tcattccagc gaaagctgaa atctcgttcc ccttggcgat ccgaagaaaa aaaagggaga
     5461 tcccagcttt cgctgggatg gcggggatcc gctgggatgg cgcgatgcgc tcggatgacg
     5521 gggatgcgcc gggaggacgg cgcgctctcc aacgagcagc cccctaaccc gcccgccgcg
     5581 cgaggttcag gtaattggga ttgaacatcc ccgccgccat cagcgcgtcg agatcgggga
     5641 tggtcagcgt ccggttctgg agctcgatta gcccctcgcg ccgcaaatct tgcaacgtgc
     5701 ggttgacgtg caccgcggtc agccccgccg cgtcggcaat gtcggcctgg gtcagcggaa
     5761 aatcgcaact gtcgccgctg gtcagtccca ccgcgcgcag ccgcaggaac atctcgcaca
     5821 acagatgcga gatccgttcg aacgccgtcc gctggccgac gttcagcgtc cattcgcgct
     5881 gcaccgccac ctcgaccagc tggtcccagg tcagcccctc ggcgatcggc gcgcgctccc
     5941 cggccagccg cgcgaattca tcgcgcggca gctcggccac cttgatcggc gtgatcgccc
     6001 cgatgctgtg gtccatcgcc cgctgcagcg cgaaatgatg gttgcacagg tcgcccggaa
     6061 ccaggaaggc gacgatctgc cgccgcccgt cgggcagcgc cttgtagcgg caggcccagc
     6121 cctccaggat caggatcagc gcgcgcggca gctcgccctc gcgcagcaaa tcgcgccggg
     6181 caggcacctc gcgcaccgat cgcgtcgcca gcaggtcgat gctcgcgcgg tcttgctcgg
     6241 ggaggggaac ataggtttcg agccggagga tcagcgggct caccagtcta attgcggttt
     6301 cgatgcggca ccttagcgtg ggcgctgacc agcgtagctt aactatgtta gcgaggtgaa
     6361 gcggtagttg ccgcaagccg ccaagcagcg tcccgtccgg cgccactaac cgcagccccg
     6421 gcgcgcgatc ggggtcctgc cgctcatcta agttaatgag aaaactggaa tcgttaaccc
     6481 gtttatcgac gccgaggata cagcgggcga ggtggtctac agcctcccgt tcaaccacgc
     6541 cctcagcatc atccccgaag gcgccgccac ggcctcgtga gccgcgacgg agcggcatcc
     6601 tgcccaatgg cgatcaccgc tcatcctgag aagggactga gccaggcgaa atgatcgggt
     6661 gagcagtacc ccgtactgcc cagccacgtc cgggggacgt ggcgacccaa tcattcccct
     6721 tttttcttgt ctccctcaca tcattgtcgc cgcgccgtaa ccctgatcgg gcaaggcggg
     6781 cgcgaaaagg aagaggaaga caagatgttc gcgagaacgg acaggttatt gttaaggccg
     6841 ggctgggcgg aggacgcccc tgccctttat gcggccatcg ccgacgaagc gatcgtgcgc
     6901 aacctggcga gtgcgccgtg gcccta
//
LOCUS       DASVRK010000234         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_420523, whole genome shotgun sequence.
ACCESSION   DASVRK010000234 DASVRK010000000
VERSION     DASVRK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420523"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VF589_09295"
     CDS             <1..1178
                     /locus_tag="VF589_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX8257818.1"
                     /translation="MNAPGFALPEPAYFNPIAYGADGPGDIVSWTINFIFFDGKMRGL
                     FSLLFGASLLLITDAAEAKGERAARVHYSRMLWLAVFGSLHFYLIWWGDILSLYASLG
                     MLAWFFHDQPPRALIRWGTALVLVQFLAFALLTLSFFEASAAALAPGADAAAVGDWTA
                     MKAEFAVPDAAELARTMALYTGSYAGIVGFRLSEELFEPLTTLIFYGWETLGYMLLGM
                     AALKTGFLTGEWSVASYRRVAAIGFAVTVPAYALLAWLLWQDGFSVQMLFALAMTVPI
                     LLRPVMVVAIAALVILITRGGGALVDWIAAAGRAAFTNYLGTSLLMTGLFYGYGLGFY
                     GTLSRAELWLVVIAAWALMLLWSKPWLDRYLYGPFEWVWRSLARGKLQPVRRRSAAT"
     gene            complement(1183..1614)
                     /locus_tag="VF589_09300"
     CDS             complement(1183..1614)
                     /locus_tag="VF589_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8257819.1"
                     /translation="MAEEAEAIVDWAAFSRTRSELGPGFVRILGYFREDGEKSVASIE
                     EAMQHKDAAALVIPAHKLKSEARQFGAEPLGELAEDIEYAARRAVEMRIFPDDLLPAV
                     ARLRPLYAQTMELFERETNPLVERRPGFGRSTAGNQDFGRI"
     gene            complement(1702..3048)
                     /gene="der"
                     /locus_tag="VF589_09305"
     CDS             complement(1702..3048)
                     /gene="der"
                     /locus_tag="VF589_09305"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX8257820.1"
                     /translation="MAKLPTVAIVGRPNVGKSTLFNRLVGRKLALVDDRPGVTRDRRE
                     AEASLLGLEFRVIDTAGYEDEDPETLPGRMRAQTTAAVREADVALFLIDARAGLTPLD
                     EEIGRWLRSETTPVVLAANKAEGRAGEAGIMEAYALGLGEPIAMSAEHGEGVVDLFEH
                     IRPYVERGEDEAEEEEGEGPLKLAIVGRPNAGKSTLINRMLQQERLITGPEAGITRDS
                     IAIDWAWQGRPVRLIDTAGMRKKAKVEDKLEKLSVADARRAVDFAEVVVLLLDGTRGL
                     ESQDLRIADNVLQEGRALVVAINKWDVAEHPSSLFNGIKGALDEGLSQARGVPLLTVS
                     GMTGKGIDQLLAAAFEIREIWSKRVSTGQLNRWFEQAVENNPPPAPGGRRIKLRYITQ
                     VNTRPPSFVLFGTRLDQLPDSYLRYLINGMRKELGFGAVPVRLTARASRNPFDKDR"
     gene            complement(3095..>4128)
                     /locus_tag="VF589_09310"
     CDS             complement(3095..>4128)
                     /locus_tag="VF589_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8257821.1"
                     /translation="GGSKRARLASAPVVGGGRIYTIDTQATVRAFDAATGGTVWSTRI
                     AGAGNNPRSLFGGGVSFANGRVYASNGTGQAAAIDAATGAVIWKVTPGGPLRGEPTVD
                     ENNIYVVSQDNQLFALNPQNGETRWTESGTVELAGVFGTAAPAASAGTVVAGFSSGEL
                     TAYRYENGRVVWQDALARTSISTSVSSLSDIDADPVIDNGRVYAVGQGGRMVALELNT
                     GQRIWEINVAGISTPWVAGEWIFVVTDQAQLLAIARASGRVKWMTQLPRWRDAEDKEG
                     PVSWTGPVLAGDRLIVASSEGHLTNVSPVDGSFLSTVSTKAPVSLSPVVAGNTLYVLH
                     DNGQLSAWR"
BASE COUNT          571 a         1453 c         1384 g          720 t
ORIGIN      
        1 cgatgaacgc ccccggcttc gcgctgcccg agccggctta tttcaacccg atcgcctacg
       61 gcgcggacgg accgggcgac atcgtctcct ggacgatcaa cttcatcttc ttcgacggca
      121 agatgcgcgg gctgttctcg ctcctgttcg gcgcgagcct gctgctcatc accgacgctg
      181 cagaagccaa aggcgagcgc gccgcgaggg tgcattacag ccgcatgctg tggctggccg
      241 tgttcggctc gctgcacttc tacctgatct ggtggggcga catcctgagc ctctacgcgt
      301 ccctgggcat gctcgcctgg ttcttccacg accagccgcc gcgggcgctg atccgctggg
      361 gcacggcttt ggtgctggtc cagttcctgg ccttcgccct gctcacgctc agcttcttcg
      421 aagcgagcgc cgcggccctg gctccgggcg ccgatgcggc ggcggtcggc gactggaccg
      481 ccatgaaggc ggaattcgcg gtccccgacg cggccgaact cgcccggacc atggcgctct
      541 acaccggcag ctacgccggc atcgtcggct tccgcctgag cgaagagctg ttcgagccgc
      601 tcaccaccct gatcttctac ggctgggaga cgctcggcta catgctgctg ggcatggcgg
      661 ccctcaagac cggcttcctg accggcgagt ggagcgttgc gagctaccgg cgcgtcgccg
      721 cgatcggttt cgccgtcacc gtccccgctt acgccctgct cgcctggctg ctctggcagg
      781 atggcttcag cgttcagatg ctgttcgcgc tggcgatgac ggtgccgatc ctgctccgtc
      841 cggtgatggt ggtcgcgatc gccgctctgg tcatcctcat cacccgcggc ggcggcgcgc
      901 tggtcgactg gatcgccgcc gccggccgcg ccgccttcac caattacctc ggcaccagcc
      961 ttctcatgac cggcctcttc tacggctacg ggctcggctt ctacggcacg ctcagccgag
     1021 ccgaattatg gctggtggtg atcgccgcat gggcgctgat gctactctgg tccaagccgt
     1081 ggctggatcg ctacctttac ggcccgttcg aatgggtgtg gcggagcctc gcccgcggca
     1141 agctccagcc ggtgcgccgc cgtagcgccg cgacctagcc gatcagatcc ggccgaaatc
     1201 ctgattgccg gcggtgctgc ggccgaatcc agggcggcgt tcgaccagcg gattggtctc
     1261 gcgctcgaac agctccattg tctgagcgta gagcggccgc aggcgggcca cggccgggag
     1321 caggtcgtcg gggaagatcc gcatctcgac cgcccgtcgg gcggcatatt cgatgtcttc
     1381 ggcgagctcg ccgagcggct cggcgccgaa ctggcgggcc tccgacttca atttatgggc
     1441 ggggatcacc agggcggccg cgtccttgtg ctgcatcgct tcctcgatgc tggcgaccga
     1501 cttttcgcca tcctcgcgaa aatagccgag gatgcggacg aagccgggcc cgagttcgct
     1561 gcgggtgcgc gaaaaggccg cccaatccac gatcgcctcg gcttcctcgg ccacccttgt
     1621 tcctcttgtc atccggttcg ctcagcccct cttacggcgc ggggagtaaa caggtcattg
     1681 acaggggcct cgccgtcgct ctcaccggtc cttgtcgaac gggttgcgcg acgcgcgcgc
     1741 cgtcagccgg accggcaccg cgccgaagcc gagctccttg cgcatgccgt tgatcaaata
     1801 gcgcagatag gaatcgggca gctggtcgag gcgggtgccg aacagcacaa acgatggcgg
     1861 ccgcgtattc acctgggtga tatatctgag cttgatgcgg cggccgccgg gcgccggcgg
     1921 cgggttgttc tcgaccgcct gctcgaacca gcgattgagc tggccggtcg acacgcgctt
     1981 cgaccagatt tcgcggatct cgaaggcggc ggcgagcaat tgatcgatgc ccttgccggt
     2041 catgcccgac acggtcagca gcggcacgcc gcgggcctgg ctgaggccct cgtcgagcgc
     2101 ccccttgatg ccgttgaaca ggctgctcgg atgctccgcc acgtcccatt tgttgatcgc
     2161 gacgaccaaa gcgcgccctt cctggagcac attgtcggcg atcctgagat cctgcgattc
     2221 gagcccgcgc gtcccgtcga gcagcagcac gacgacctcg gcgaagtcca ccgcccggcg
     2281 ggcgtcggcg accgagagct tttccagctt gtcctcgacc ttggctttct tgcgcatccc
     2341 ggcggtgtcg atcagccgca ccggccgccc ctgccaggcc cagtcgatcg cgatcgaatc
     2401 gcgggtgatg ccggcctcgg gcccggtgat cagccgctcc tgctgcagca tgcggttgat
     2461 cagcgtcgat ttgccggcgt tcggacgtcc gacgatggcg agcttgagcg gcccctcgcc
     2521 ttcctcctct tccgcctcgt cctcgccgcg ttcgacatag gggcggatat gctcgaacag
     2581 atcgaccacg ccttcgccat gctcggcgct catcgcgatc ggctcgccca ggcccagcgc
     2641 ataggcctcc atgatgccgg cctcgccggc gcggccctcg gccttgttgg cggcgagcac
     2701 gaccggcgtg gtctcgctcc gcagccagcg gccgatctcc tcgtcgagcg gggtcagccc
     2761 ggcgcgggcg tcgatcagga acaaggcgac gtcggcctcg cgcaccgcgg ccgtggtctg
     2821 cgcgcgcatc cgccccggca gcgtctcggg atcttcatcc tcgtagccgg cggtgtcgat
     2881 cacgcggaat tcgaggccga gcaggctcgc ctcggcctcg cgccggtcgc gcgtgacgcc
     2941 gggacggtcg tcgacgaggg cgagcttgcg cccgaccagc cggttgaaca gcgtggactt
     3001 gccgacattg ggtcggccga cgatcgcgac ggtgggcagc ttggccatca ggcggccgcc
     3061 ctgccgcctt ggaccaagcc ggtcaaccgc tgcgtcagcg ccaggcgctc agctggccgt
     3121 tatcgtggag cacgtagagc gtgttccccg ccaccaccgg cgagagcgac accggcgcct
     3181 tggtcgacac cgtcgatagg aagctgccgt caaccggcga gacgttggtc agatgccctt
     3241 ccgaactggc gacgatcagc cggtcgccgg ccagcaccgg accggtccag ctgaccgggc
     3301 cctccttgtc ctcggcgtcg cgccagcgcg ggagctgcgt catccacttc acgcggccgc
     3361 tggcgcgggc gatggcgagc aactgcgcct ggtcggtgac cacgaagatc cactcgcccg
     3421 cgacccaggg cgtcgagatg ccggcgacgt tgatctccca gatgcgctgg ccggtattga
     3481 gctcgagcgc gaccatgcga ccgccctggc cgacggcata gacgcggcca ttgtcgatca
     3541 ccggatcggc gtcgatgtcg gagaggctgg agaccgaggt cgagatgctg gtccgggcga
     3601 gcgcgtcctg ccacacgacg cggccattct cgtagcggta ggcggtgagc tcgcccgacg
     3661 agaagccggc caccaccgtg cccgccgacg ccgccggggc ggcggtgccg aacacgccgg
     3721 ccagttcgac ggtgccggat tcggtccagc gggtttcgcc attttgcggg ttgagcgcga
     3781 acagctggtt gtcctggctg accacataga tgttgttttc atcgaccgtc ggctcgccgc
     3841 gcagcgggcc gcccggcgtc accttccaga tgacggcgcc ggtggcggcg tcgatcgccg
     3901 ccgcctggcc ggtgccgttg ctggcgtaga cgcggccgtt ggcgaagctg acgccgccgc
     3961 cgaacaacga gcgcgggtta ttgcccgcgc ccgcgatccg ggtcgaccag acggtgccgc
     4021 cggtggcggc gtcgaaggcg cgtaccgtcg cctgggtgtc gatcgtgtag atcctgccgc
     4081 cgcccaccac gggcgcggaa gcgaggcgcg cgcgcttgct gccgccct
//
LOCUS       DASVRK010000235         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_154308, whole genome shotgun sequence.
ACCESSION   DASVRK010000235 DASVRK010000000
VERSION     DASVRK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154308"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VF589_09315"
     CDS             <1..950
                     /locus_tag="VF589_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX8257822.1"
                     /translation="EWQQLEGILQVAERKSVRALSDDDLLALPVLYRGALSSLSVARE
                     TSLDLELVTYLEGLCARAYFFVYGVRTSPWRRMGVFFGSDWPEAVRSLWKETIVAMLV
                     MLAGALAGFLLVAGDPSWFGAFVPEELAGGRTFDAPAEELRSTLYDRGEGTDLLGAFA
                     ALLFTHNSQVAILAFALGFAFGIPTLLLLVYNGTTLGAFVALFVSHGLGAELGGWLII
                     HGSTELFAIALAGAAGLRIGSAVVFPGERARMAAAAVAGRTAAIAMAGVLIMLFVAGL
                     LEGIGRQTITSDAARYAIGIGVLMLWFGFYYLPRRRADG"
     gene            943..1827
                     /locus_tag="VF589_09320"
     CDS             943..1827
                     /locus_tag="VF589_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX8257823.1"
                     /translation="MAKAKAMRGDAEAKVRRSLVTPEGVDLSLRLAEPGQRFAAFLID
                     LLIMAGLLIAATLLIVLGTATIGVQSAGLALILWLLGFFLLRNFYFIVLEMGPRAATF
                     GKRAIGLRVVARSGGRLTADAVIARNLLRELEFYLPMSFLVYRAAEGSADAAMAAFGF
                     GWTCIFLFFPFFNRDRLRVGDLLAGTWVINAPKRRLGTELLGAAAAGDAPAYSFTAEQ
                     LEAYGVFELQTLENVLRRANPDAMGTVVYTIRNRIGAVQHGGDEEFLRAYYEALRLRL
                     ERQLLFGRRRRDKHDLAG"
     gene            complement(1831..2313)
                     /locus_tag="VF589_09325"
     CDS             complement(1831..2313)
                     /locus_tag="VF589_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8257824.1"
                     /translation="MTIAEWCLFGAVMLYLLTIAPAKAIGHRDFDNASPRDPAFYEHP
                     VRKRALGAHINGLETFPFFAAAVLLAEFRQAPQPWIDALALGFLAARIAFVAAYLADK
                     ATLRTMLWNVAMAFNIGIFFLPAVDLQGLAVVMAIALGWALLLWPILAAVERGRAARR
                     "
     gene            2495..3205
                     /locus_tag="VF589_09330"
     CDS             2495..3205
                     /locus_tag="VF589_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8257825.1"
                     /translation="MDKERKVAIVTGASQGIGAALVDAYLERGYRVVAVSRSIKPSAD
                     PDVLTIAGDIGDPATGRRAVDEGLARFGRIDTLVNNAGIFVAEPFTRYTAGQYAEVMS
                     TNVNGFFFITQPAIEAMEKQGSGHVVSITTALVDHPSAKVPSVLASLSKGGIAAASKS
                     LAIEYATRGIRVNAVSPGVIKTPMHPAETHEFLSALHPVGRMGEIEDVVGAVLFLETA
                     PFVTGEVLHVDGGQSAGF"
BASE COUNT          475 a         1088 c         1139 g          544 t
ORIGIN      
        1 cggaatggca gcagctcgaa ggaattttgc aggtggccga gcgcaagtcg gtgcgcgcgc
       61 tcagcgacga cgacttgctc gcgctgccgg tgctgtaccg gggcgcgctc tcctcgctgt
      121 cggtggcgcg cgagacctcg ctcgacctcg agctcgtcac ctatctcgag gggctgtgcg
      181 cgcgcgccta tttcttcgtc tacggggtca ggacctcgcc atggcggcgg atgggcgtct
      241 tcttcggctc cgactggccc gaagccgtcc gcagcctgtg gaaggaaacc atcgtcgcaa
      301 tgctggtgat gctggcgggc gcgttggccg gcttcctgct ggtcgccggc gatccgagct
      361 ggttcggcgc cttcgttccc gaggagctgg ccggcgggcg caccttcgat gcaccggccg
      421 aggagcttcg aagcacgctc tacgaccgcg gcgaggggac cgacctgctc ggggccttcg
      481 ccgccttgct cttcacccat aacagccagg ttgcgatcct ggcgttcgcg ctcggcttcg
      541 ccttcggcat ccccaccctg ctgctgctcg tctataacgg caccaccctc ggcgccttcg
      601 tcgccttgtt cgtgtcccac gggctcggcg cggagctcgg cgggtggctg atcatccacg
      661 gctcgaccga attgttcgcg atcgcgctgg ccggcgcggc gggcctccgg atcggatcgg
      721 cggtcgtgtt ccccggcgag agggcgcgca tggccgcggc ggcggtggcc gggcggaccg
      781 ccgccatcgc catggccgga gtcctgatca tgctgttcgt cgccggcctg ctcgaaggca
      841 tcgggcggca gacgatcacc agcgatgcgg cccgctacgc gatcggcatc ggcgtgctga
      901 tgctctggtt cggcttctat tatctgccgc ggcggcgggc cgatggctaa ggccaaggcg
      961 atgcgcggcg acgcggaggc caaggtccgg cgcagcctcg tcacgccgga aggggtcgac
     1021 ctcagcctgc ggctcgccga gcccggccag cgcttcgccg cctttttgat cgatcttctg
     1081 atcatggccg ggctgctgat cgccgccact ttgctgatcg tgctcggcac cgcgacgatc
     1141 ggcgtccaga gcgcggggct cgccctcatc ctgtggctgc tcggtttctt cctgctccgg
     1201 aatttctact tcatcgtgct ggaaatgggg ccgcgcgcgg ccaccttcgg caagcgcgcg
     1261 atcgggcttc gcgtggtggc gcgcagcggc ggccgcctga ccgccgacgc ggtgatcgcg
     1321 cgcaacctgc tgcgcgagct ggaattctat ttgccgatgt cattcctcgt ctaccgggcc
     1381 gccgaaggct cggccgacgc ggcgatggcg gcgttcggct tcggatggac ctgtatcttt
     1441 ctgttctttc cgtttttcaa ccgggaccgg ctgcgggttg gggacctgct tgccggcacc
     1501 tgggtgatca atgcgcccaa gcgccggctc ggcaccgaat tgctcggcgc agcggcggcc
     1561 ggcgacgcgc ccgcgtacag cttcaccgcc gagcagctcg aggcttatgg cgtgttcgag
     1621 ctgcagacgc tggagaatgt gctgcgcagg gccaatccgg acgcgatggg cacggtcgtc
     1681 tacaccatcc gcaaccggat cggcgcggtg cagcatggcg gcgacgagga attcctgcgc
     1741 gcttattacg aggcgctgcg gctccggctc gagcgccagc tgctgttcgg ccgccgccgc
     1801 cgcgacaagc acgacctcgc cgggtgagga tcagcgccgg gccgcccggc cgcgctcgac
     1861 ggcggccagg atcggccaga gcagcagcgc ccagccgagc gcgatcgcca tcaccaccgc
     1921 cagcccctgg aggtcgacgg cggggaggaa gaagatgccg atgttgaacg ccatggcgac
     1981 gttccatagc atggtgcgca aggtggcctt gtcggcgaga taggcggcga cgaaggcgat
     2041 gcgggcggcg aggaagccga gcgccagcgc gtcgatccat ggctgcgggg cctgcctgaa
     2101 ttcggccagc agcaccgcgg cggcgaagaa ggggaaggtt tcgaggccgt tgatatgggc
     2161 gccgagcgcg cgcttcctga ccggatgctc gtaaaaggcc gggtcgcgcg gcgaagcatt
     2221 gtcgaagtcg cggtggccga tcgccttggc cggggcgatg gtcagcaaat agagcatgac
     2281 ggcgccgaac aggcaccatt ccgcgatcgt cattgcagtc tccccttggc ccatgccggc
     2341 ggtgcattgc agcaggtccg gccgggaatt gccagccgcc ggcaccgcat tcttcgccga
     2401 atatttccgc gggggcgtaa ccgcggcggg ccggcgcgag aaatgccgat cgggcgccga
     2461 tcgaagcggc gcgagcatcg gaaaggaccg catcatggac aaggaacgca aagtcgccat
     2521 cgtcaccggc gcatcccagg gcatcggcgc ggcgctggtc gacgcctatc tggagcgcgg
     2581 ctaccgcgtg gtcgccgtgt cgcgctcgat caagccgtcc gccgacccgg acgtgctgac
     2641 gatcgccggc gacatcggcg atccggcgac cggccggcgg gcggtcgacg aagggctggc
     2701 gcgcttcggg cggatcgaca ctttggtcaa caatgccggc atcttcgtgg cggagccgtt
     2761 cacccgctat accgccggtc aatatgctga ggtcatgtcg accaacgtca acggcttctt
     2821 cttcatcacc cagccggcga tcgaggcgat ggagaagcag ggcagcggcc acgtcgtcag
     2881 catcaccacg gccctggtcg accatccgag cgccaaggtg ccgtcggtgc tcgcctcgtt
     2941 gagcaagggc gggatcgcgg cggccagcaa gagcctggcg atcgaatatg ccacccgcgg
     3001 gatccgcgtg aacgccgtct cgcccggcgt catcaagacg ccgatgcacc cggccgagac
     3061 gcacgaattc ctgtcggcgc tgcacccggt cgggcggatg ggcgagatcg aggacgtggt
     3121 cggcgccgtg ctgttcctcg agaccgcgcc gttcgtgacc ggcgaggtcc ttcacgtcga
     3181 cggcggccag tccgccggct tctgatcgac ccccgcgaac gccaaggaga cacgtcatgc
     3241 cgatcg
//
LOCUS       DASVRK010000236         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_42467, whole genome shotgun sequence.
ACCESSION   DASVRK010000236 DASVRK010000000
VERSION     DASVRK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42467"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..583)
                     /locus_tag="VF589_09335"
     CDS             complement(17..583)
                     /locus_tag="VF589_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX8257826.1"
                     /translation="MDALLTTFIAAALAEIGDKTQLLVIALAARYGRPKPVLAGVAVA
                     ALLNCAIAALGGTLIHDFITLRAISLLVALALVFTGVSGLIRSGQREVGANWKEDAFA
                     TTVACFFVLELGDKTQFLTAALAARYDSLVLAGLGATLGVVAANAPGALLGDRLTAFV
                     SLRMVRMTIAILFLIAGFIVGVDALRLV"
     gene            complement(621..941)
                     /locus_tag="VF589_09340"
     CDS             complement(621..941)
                     /locus_tag="VF589_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257827.1"
                     /translation="MRAEQQDAMMLVRAELCRRLDRLEAPAGRRSPAELSGAIAAIGH
                     LAAAYGLTPVVRLAEAAQRALAQGGCPAALYLDRLHDAIGCTRNDEQAGEAMIASVSV
                     RLTA"
     gene            1117..1686
                     /locus_tag="VF589_09345"
     CDS             1117..1686
                     /locus_tag="VF589_09345"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8257828.1"
                     /translation="MQATATPDEIIEQAVAAIGGGEAAMRAALDDLPAPIYTTDRDGR
                     VTFFNQACIGFAGRTPVTGEDRWCVTWKLYTTDGAALPHEQCPMAVAIRERRPVRGAE
                     AIAERPDGSRVSFVPYPTPLFDAAGSLIGAVNMLIDVTVSRQADHLRAQAVRCRRLAA
                     SVNDRPAVATLTAMAAEYEDQARALQRPH"
BASE COUNT          324 a          654 c          743 g          263 t
ORIGIN      
        1 ggcggcggcg cggccgtcag accagccgca gcgcgtcgac accgacgata aagccggcga
       61 tcaggaacag gatggcgatg gtcatgcgca ccatgcgcag cgagacgaag gcggtgaggc
      121 ggtcgccgag caacgcgccc ggagcgttgg cggcgaccac gccgagggtg gcgccgagcc
      181 cggccagcac cagcgaatcg tagcgggcgg cgagcgcggc ggtgaggaac tgggtcttgt
      241 cgccgagctc gagcacgaag aagcaggcga cggtggtggc gaaggcgtcc tccttccaat
      301 tggcgccgac ctcgcgctgg ccgctgcgga tgaggccgct cacgccggtg aagaccagcg
      361 ccagcgcgac cagcagcgag atggcgcgca aagtgatgaa atcgtggatc aaggtgccgc
      421 cgagcgcggc gatcgcgcaa ttgaggagcg cggcgacggc gactccggcc agcaccggct
      481 tcggacggcc gtaacgcgcg gcgagcgcga tcacgagcag ctgcgtcttg tcgccgatct
      541 cggcgagggc ggcggcgatg aaggtggtga ggagggcgtc catcgggctc gcttcggcgc
      601 tcgcttcggc gcccgggggt tcaggcggtg agccgcaccg ataccgaagc gatcatcgcc
      661 tcgccagcct gttcgtcgtt acgggtgcag ccgatcgcat cgtggaggcg atcgagatag
      721 agcgcggccg ggcagccccc ttgcgccagc gcgcgctggg cggcttcggc gaggcgcacg
      781 acgggcgtga ggccgtaagc ggcggcgaga tggccgatgg cggcgatcgc gccggaaagc
      841 tcggcgggcg agcggcggcc ggcgggcgct tcgagacggt cgagccgccg gcacagctcg
      901 gcccggacca gcatcatcgc atcctgttgt tccgcccgca tcgcacggct cccctggtgt
      961 ccccaaggag agcatgcgcc gcgcgggtta cagagcggtt aacgaatgag gcggccggcg
     1021 gcggggcggc ggcgctttct cggggaactt tcggggctga tctgggttaa catctggcca
     1081 cgggggcact ccatccaccg cgtatgccgg actgaaatgc aagccacagc cacgcccgac
     1141 gaaatcatcg aacaggcggt cgccgcgatc ggcggcggcg aggccgcgat gcgggccgcg
     1201 ctcgacgacc tgccggcgcc gatctacacc accgaccggg acgggcgggt gaccttcttc
     1261 aaccaggcct gcatcggctt tgccggccgc accccggtta ccggcgagga ccgctggtgc
     1321 gtgacctgga agctctacac caccgatggc gcggcgctgc cgcacgagca atgcccgatg
     1381 gcggtggcga tccgcgagcg gcggccggtg cgcggcgccg aggcgatcgc cgagcggccg
     1441 gacggcagcc gggtgagctt cgtcccctat ccgacgccct tgttcgacgc cgcgggctcg
     1501 ctcatcggcg ctgtcaacat gctgatcgac gtcaccgtca gccgccaggc cgatcacctg
     1561 cgcgcgcagg cggtgcgctg ccggcggctg gcggcctcgg tcaacgaccg gccggcggtg
     1621 gcgacgctga ccgcgatggc ggccgaatat gaggaccagg cgcgggcgct gcagcggcca
     1681 cattgaggtg gcggtggggg cggcgggccg gtcgattcca tggccgcctc gcttctgttc
     1741 gatcctgcca agaagaagtg ggatgccatg atcgggtccc ctaatgtgct gtttgggggg
     1801 acaagcccac gaccattatt caggatgagc ggcaagggcg ctgaccgtgc ccctgcgaag
     1861 gcaggggccc agaccaggcg cagcctgtca aacgagggcc gcgccagcgc gggttcatcc
     1921 gctcgacggt gccgagcttc cacagccgct tcgcatatcc gaccttcatc caaagaaaac
     1981 gacc
//
LOCUS       DASVRK010000237         4190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_482488, whole genome shotgun sequence.
ACCESSION   DASVRK010000237 DASVRK010000000
VERSION     DASVRK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482488"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VF589_09350"
     CDS             <1..1048
                     /locus_tag="VF589_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257829.1"
                     /translation="NTILLMRHYFLWARITATNPATGEITLDEPVEFFGNGVIGNANN
                     IGTFNNNNTQPVTEQPMYLLEDATITGTGGFESKGASIARTACRNVSVHLGSNKGLYG
                     MGGNAVGDSDFYFENITALNKLWDVAAQSYRSTFGFGTATLLMTEPPTGNDQAEKSKG
                     ASSIGENSRQITITGGTIDATAFTGDTGLLHFNVCRGSSMAFLTVNVPNASGAMISVG
                     SAVDYWVGTVRPNINDNSFRCVDLQARPGAGVGDVSFLHVSDLRSDGTTATDLKRFSL
                     LGGTFRGQATKQSLLRGQDHLIGPDVRIESNGVNVDNAGSYATFDRCHIAGGITFTDG
                     ALQSQSTIINVTTD"
     gene            complement(1165..1893)
                     /locus_tag="VF589_09355"
     CDS             complement(1165..1893)
                     /locus_tag="VF589_09355"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX8257830.1"
                     /translation="MKHIGILAHSAEGATLCYRTAWMEGVRRLGPHHHPEITLTGVAM
                     HHAMPAWESGDLASLRALFMADAAKLAAAGADFFILPDNTAHIALEAPGEPFPIPCLH
                     IGETVADQAVREGRRRIAILGTNWTMEGPVYPAAFARRGLAWEVPEPEDRALIHRIIF
                     DELCLGVFTNASRAAFVAVIDRMAQRGCDAAALVCTEIPLLVTPEASPLPTLDSTRLL
                     AAAAVEVAVGQRPMPDWRGGSVGG"
     gene            complement(1934..2716)
                     /locus_tag="VF589_09360"
     CDS             complement(1934..2716)
                     /locus_tag="VF589_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8257831.1"
                     /translation="MRLDQDIAAIVTGAASGLGEATARALAAKGVRVAVFDRDREKGE
                     RVAAEIGGIFALTDVTSDEAVEAAFAAARAAHGQERICVNCAGVANAVKTVGRDKETK
                     AVKRYPIHQFELAVQINLIGTFRVLAASAAGMAELDPLEDGEKGVIINTASVAAQDGQ
                     IGQAAYSASKAGVLGMALPIARDLMSEGIRVNTILPGVFKTPMVAMMPPNVQDALAAQ
                     VPFPKRLGQPEDYARLALFMAENSYLNGEYVRLDGAIRMAPR"
     gene            2899..>4190
                     /locus_tag="VF589_09365"
     CDS             2899..>4190
                     /locus_tag="VF589_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8257832.1"
                     /translation="MTSGIRKWALRAAAAPLLALAISPSAASAETAPALRAALQAAAE
                     PGLARFYGPRHYRPIWVKNGFLVPEAARMIAMTRDAELDGMVSARELASELERAVNRA
                     GSGDPRDQAYAELMLSRALVGYVETLRRPADAGAGMVYADSAVAPSVPFRTAILSDAA
                     AAPSLSAHLDKVSTVNPLYSQLRSALATLRDQPARVFDAAATSGFGEDPRQIERRLKL
                     NLERARALPVSQSGRYVLVDAVSARLWLYEDGKPRDSMKVIIGKPSEPTPMVASMIRT
                     AVLNPYWNVPPDLVRKKVAPKVLEEGLGYLKKSRYEVLSDWTENAVAVDPETIDWQAV
                     AAGHEELRVRQLPGAGNSMGDMKFMFPNEFGVYLHDTPDKALFAQADRKLSSGCVRVE
                     DAARLAKWLFGTTPRAPSSAPEQVVALPEPVPVYITYLT"
BASE COUNT          676 a         1401 c         1472 g          641 t
ORIGIN      
        1 gaacacgatt ctgctcatgc ggcactactt cctgtgggct cgcattacgg ccaccaatcc
       61 ggcgaccggt gagatcacgc tggacgagcc ggtggagttc ttcggcaacg gggtgatcgg
      121 caacgcgaac aatatcggta ccttcaataa taacaacacg cagccggtga ccgagcagcc
      181 gatgtacctt ctcgaagacg ccaccatcac tggcaccggc gggttcgaat cgaagggagc
      241 ctccatcgcg cggacggctt gccgcaacgt gtcggtgcat ctcggctcca acaaaggtct
      301 gtacggcatg ggcggcaacg ccgtcggcga cagcgatttc tacttcgaga acatcacggc
      361 cctgaacaaa ttgtgggacg tcgccgccca aagctaccgg tccacattcg ggttcgggac
      421 ggccacgctc ctgatgaccg agcctcccac gggcaatgat caggccgaga aaagcaaagg
      481 ggccagcagc atcggggaga acagccggca gatcacgatc acgggcggga ccatcgatgc
      541 tacggcgttc accggcgaca cgggcctgct gcatttcaac gtctgccgtg gaagctcgat
      601 ggctttcctc accgtcaacg tgccgaatgc gtcgggagcg atgatcagcg tgggctccgc
      661 cgtggactat tgggtgggca ccgtcaggcc gaacatcaac gacaacagct tccgctgcgt
      721 tgatctccag gcgcgcccgg gcgcgggtgt gggggacgtc agcttcctcc acgtctcgga
      781 ccttcggtcg gacggcacga cggctacgga cctgaagcgc ttcagcctgc tcggcgggac
      841 cttccgcggc caggcgacga agcaaagcct gttgcggggg caggaccatc tgatcggccc
      901 cgacgtgagg atcgagtcga acggcgtcaa cgtcgacaat gcaggatcct acgccacgtt
      961 cgaccgctgc cacatcgccg gcggaataac gttcaccgat ggcgcgctgc agagccaaag
     1021 caccatcatc aacgtgacca ccgactgacg aggcggccac gttcgctcgg ccgcaggtcg
     1081 cggctgacga atagcggggg aaggcggtcc gcaccgcctt cccccttttt tttgcgagta
     1141 cgatcgggcc gccgggggtg ccggctatcc gccgacgctg ccgccccgcc agtccggcat
     1201 cggccgctgg ccgaccgcca cctcgaccgc cgccgccgcc agcaggcggg tggaatcgag
     1261 cgtcgggagc ggcgaggctt ccggcgtcac gagcagcggt atttcggtgc agaccagggc
     1321 ggcggcgtcg cagccgcgct gcgccatccg atcgatgacc gccacgaagg cggcgcgcga
     1381 ggcgttggtg aagacgccga ggcagagctc gtcgaagatg atgcggtgga tgagcgcgcg
     1441 gtcctcgggc tcgggcacct cccaggcgag gccgcggcgg gcgaaggcgg ccgggtagac
     1501 cggcccctcc atggtccagt tggtgccgag gatggcgatc cgccgccgcc cctcgcgcac
     1561 cgcctggtcg gccaccgtct cgccgatgtg gaggcagggg atcgggaacg gctcgcccgg
     1621 cgcttcgagc gcgatatggg cggtgttgtc gggcaggatg aagaaatcgg cgccggccgc
     1681 ggcgagcttg gcggcatcgg ccatgaacaa agcgcggagc gaggcgaggt cgccggattc
     1741 ccaggccggc atggcatggt gcatcgccac gccggtgagc gtgatctcgg gatgatgatg
     1801 gggcccgagc cggcgcacgc cctccatcca ggcggtgcgg tagcataagg tcgccccttc
     1861 cgcgctgtgg gcgaggatgc cgatatgctt catgccgttc cccccggcgc ggtcggcgcg
     1921 gcgggatgac ggctcaccgc ggcgccatcc ggatggcgcc gtcgagccgc acatattcgc
     1981 cgttcagata gctgttctcg gccatgaaga gggcgagccg cgcatagtcc tcgggctggc
     2041 cgagccgctt cgggaacggc acctgcgcgg ccagcgcgtc ctggacgttg ggcggcatca
     2101 tcgccaccat cggcgtcttg aacacgcccg gcaggatggt gttgacgcgg atgccctcgc
     2161 tcatcaggtc gcgcgcgatc ggcagcgcca tgccgagcac gcccgccttg gacgcgctgt
     2221 aggcggcctg gccgatctgg ccgtcctggg cggcgaccga ggcggtgttg atgatcacgc
     2281 ccttctcgcc atcctcgagc ggatcgagct cggccatgcc cgcggccgag gcggcgagca
     2341 cgcggaaggt gccgatgagg ttgatctgga cggcgagctc gaactggtgg atcgggtagc
     2401 gcttgaccgc cttggtctcc ttgtcgcggc cgaccgtctt caccgcgttg gcgacgcctg
     2461 cgcaattgac gcagatccgc tcctggccgt gggcggcgcg ggcggcggcg aaggcggcct
     2521 cgaccgcttc gtccgacgtg acgtcggtga gcgcgaagat gccgccgatc tcggcggcga
     2581 cgcgctcccc tttctcgcgg tcgcggtcga acacggcgac cctgacgcct ttggcggcga
     2641 gcgcgcgggc ggtcgcctcg ccgagcccgg aggctgcgcc ggtgacgatg gcggcgatat
     2701 cctggtctaa tctcatgctt ggctcccgtt cgcgtgcgcg ccgctcctag ggcgggacgg
     2761 ggcggttgga caaggctcgg cggcgcccca tatcggaggg cgcagcgtcc tgaagcgggg
     2821 cggcgccgac aagccgtgga ccccgaaggg catatgatgc gtttcggctg cagtaacgag
     2881 taacaaaaga gcaaaaaagt gacatcaggg atcagaaagt gggcgttgcg cgccgccgcg
     2941 gcgccgctgc tagcactcgc catatcgccg tccgcggctt cggccgagac cgcgcccgcg
     3001 ctccgggccg cgttgcaggc ggccgccgag ccgggcctcg cccgtttcta cggcccgcgc
     3061 cactatcgcc cgatctgggt caagaacggt ttcctcgtgc ccgaggcggc gcggatgatc
     3121 gccatgacgc gcgatgccga gctcgatgga atggtttcgg cgcgcgagct cgcgtccgag
     3181 ctcgagcggg cggtcaaccg cgccggatcg ggcgacccgc gcgaccaggc ttatgccgag
     3241 ctgatgctgt cgcgggcctt ggtaggctat gtcgagacgc tgcgccgccc ggccgacgcc
     3301 ggcgccggca tggtctatgc cgacagcgcg gtcgcgccct cggttccgtt ccgcaccgcc
     3361 atcctcagcg acgccgccgc ggcgccgtcg ctctccgccc acctcgacaa ggtttccacg
     3421 gtcaacccgc tctacagcca gctgcgcagc gcgctcgcga ccttgcgcga ccagccggcg
     3481 cgggtgttcg acgccgcggc gacgagcggc ttcggcgagg atccgcgcca gatcgagcgg
     3541 cggctgaagc tcaacctgga acgcgcgcgg gcgctgccgg tctcgcagag cggccgctat
     3601 gtgctggtcg acgcggtcag cgcgcggctg tggctctacg aggacggcaa gccgcgcgac
     3661 agcatgaagg tgatcatcgg caagcccagc gagccgacac cgatggtggc gagcatgatc
     3721 cgcaccgccg tgctcaaccc ttattggaac gtgccgcccg acctcgtccg caagaaggtc
     3781 gccccgaagg tgctcgagga gggcctcggc tacctcaaga agagccgcta cgaagtgctg
     3841 tccgactgga ccgagaatgc ggtcgcggtc gatccggaga cgatcgactg gcaggcggtg
     3901 gcggccggcc acgaggaatt gagggtgcgc cagctgcccg gcgccggcaa ttcgatgggc
     3961 gacatgaagt tcatgttccc caatgagttc ggcgtctacc tgcacgacac gcccgacaag
     4021 gccttgttcg cgcaggccga ccgcaagctg agctcgggct gcgtccgggt cgaggatgcg
     4081 gcgcggctgg cgaaatggct gttcgggacg acgccgcgcg cgccttcgtc ggcccccgag
     4141 caggtggtgg cgctgcccga gccggtgccg gtctacatca cctatctgac
//
LOCUS       DASVRK010000238         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_109274, whole genome shotgun sequence.
ACCESSION   DASVRK010000238 DASVRK010000000
VERSION     DASVRK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109274"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF589_09370"
     CDS             complement(<1..345)
                     /locus_tag="VF589_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257833.1"
                     /translation="MGRPAGMIEFSPPPAEGLPLACRPSGGTDQSGPALDLLAAERPR
                     LEDRLLVHGALLLRGFGLGSVADFQRLVALFCDGDPLDYAGGASPRSRIAGASGVYTS
                     TDYPAAMALSLHN"
     gene            486..1364
                     /locus_tag="VF589_09375"
     CDS             486..1364
                     /locus_tag="VF589_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257834.1"
                     /translation="MAYFSFTDASNETFVIRVTDRDDIAHARALLAGTTTDAARVGGT
                     IIKVPADYNIGWSYKLAPDSIMFYDASIEVADSTMRYIETHLSEVGGHLLPGRQWAGW
                     STTLTSELTVRSGSWANDILHGQTEVDDLMFGRGGNDILFSYGGNDHLSGGDGNDILK
                     AARGDDKLGGGDGNDILEADWGDDFLHGGRGSDVLIGGGGDDVFLAGRFSDCDRDVIA
                     DFEGGRQLEDQIRFDRTWLREIGDVNDDGRVNRRDIAAAFEEKGEDLVLDLRGSSITL
                     ENAAGTQLHVNDFLIG"
     gene            1439..>3228
                     /locus_tag="VF589_09380"
     CDS             1439..>3228
                     /locus_tag="VF589_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8257835.1"
                     /translation="MRHGPVATTFCASALLLGGCAMNDGVTTAPAPPPAAAGPSPALA
                     AAPARYSAKTFFETTSYTMAAPAGFGFSADGSRILVNSDQSGVFNAYALAVAGGAAAP
                     LTSSADNATFAQSFFPADDRMLFTFDKGGNELNHVYVREADGSVRDLTPGDKVKADFL
                     GFSADGKTFWVTSNERDPEMFDVYAYDVDGYGRRMLFRNQGYAVGDISPDGRYLALVK
                     ERTSADNNLYLADLSAKSAPKLITTHKGNVSHAVYDFSRDGRTLVYATDEAGEFNQAW
                     AYDLASGGKKPMIAADWDVMFVQYSPSGRYRVHALNSDGSTDLTITARDGRPVALKGI
                     PDGDVGGVRFNRDETMVAFTVASDTSPADIFVADLATGQARRLTKALNPAIDEAQLVE
                     ATVARFKSYDGLEVPGILYKPREASASNKVPAVVFVHGGPGGQSRRGYSAMVQHLVNN
                     GYAVYAINNRGSSGYGKTFFHLDDRRHGDVDLKDVVASKGFLQSMDWVDGERIGIMGG
                     SYGGYMVAAALAFEPQAFDVGVNIFGVTNWVRTLQSIPPWWESMREALFDEMGDPATD
                     GERHRAISPLFHAKNIVKPMLVVQGANDPRV"
BASE COUNT          568 a         1109 c         1055 g          496 t
ORIGIN      
        1 gttgtggagc gacagcgcca tggccgccgg ataatcggtc gaggtgtaga cgccgctcgc
       61 cccggcgatg cggctgcgcg gcgaggcgcc gccggcataa tcgagcgggt cgccgtcgca
      121 gaacaaagcg accagccgct ggaaatcggc gaccgagccg aggccgaagc cgcgcaggag
      181 cagcgcgccg tggacgagca gccgatcctc aaggcgcggg cgttcggcag cgagcaagtc
      241 gagcgccgga ccggattgat cggttccgcc ggagggtcgg caggcgagcg gcaagccctc
      301 cgccggaggc gggctgaact cgatcatccc tgccggtctt cccatccttg ccgccttgtt
      361 ccggggcgaa gaacaaggga cgggcaagca ggttcaatcc gggattttgc ggcgccgcct
      421 ttgcgcttgc cgcgaccccg cctcaggccg taaattgccg caccatttta gagggggact
      481 cagcgatggc ttatttcagc tttaccgacg cttcgaacga gacgttcgtg atccgggtca
      541 ccgaccggga cgatatcgcg catgcgcggg cgctgctggc cgggaccacg accgacgcgg
      601 cgcgcgtcgg cggcaccatc atcaaggtcc ccgccgacta taatatcggc tggtcctaca
      661 agctcgctcc cgacagcatc atgttctacg acgcgtcgat cgaagtcgcc gactcgacca
      721 tgcgctatat cgagacgcac ctcagcgaag tcggcgggca ccttctcccg ggccgccaat
      781 gggcgggatg gtcgaccacg cttaccagcg aactcaccgt ccgttccggc agctgggcca
      841 acgacatcct gcatggccag accgaggtcg acgacctgat gttcggccgc ggcggcaacg
      901 acatattgtt cagctatggc ggcaacgacc atctctccgg aggcgacggc aacgacatat
      961 tgaaagccgc gcgcggcgac gacaagctgg gcggcggcga cggcaacgac atactcgagg
     1021 ccgattgggg cgacgacttc ctccacggcg gccgcggcag cgacgtgctg atcggcggcg
     1081 gcggcgacga cgtcttcctc gccggcaggt tcagcgactg cgaccgcgac gtgatcgccg
     1141 atttcgaggg cggccggcag ctggaggacc agatccgctt cgaccgcacc tggctccgcg
     1201 agatcggcga cgtcaatgac gacggcaggg tcaaccgccg cgacatcgcc gccgcgttcg
     1261 aggaaaaggg cgaggatctg gtgctcgacc tccgcgggtc gtcgatcacg ctcgagaatg
     1321 cggccggcac gcagctgcac gtcaacgact tcctcatcgg ctgatccccg ccccacgaaa
     1381 cagggctggt caagctcccg ccggtgtatt atcttactaa gacacaacgg gaggaattat
     1441 gagacatggc cctgtcgcga ccactttttg cgcctccgcg ctcctgctcg gcgggtgcgc
     1501 gatgaacgac ggcgtgacga cggcgccggc cccgccgccc gcggcggcgg ggccctcccc
     1561 ggccctggcc gcggcccccg cgcgctactc agccaagact ttcttcgaga cgaccagcta
     1621 cacgatggcg gcgccggccg gcttcggctt ctcggcggac ggcagccgga tcctggtcaa
     1681 cagcgaccaa tccggcgtgt tcaacgccta tgcgcttgcc gtggcgggcg gcgcggcggc
     1741 gccgctcaca agctcggccg acaatgccac cttcgcgcaa agcttctttc ccgccgacga
     1801 ccggatgctg ttcaccttcg acaagggcgg caacgagctg aaccacgtct atgtgcgtga
     1861 ggccgacggg tcggtgcgcg acctcacgcc gggcgacaag gtcaaggccg actttctcgg
     1921 cttcagcgcc gacggcaaga ccttctgggt gaccagcaac gagcgcgacc cggagatgtt
     1981 cgacgtctac gcctatgacg tggacggcta cgggcgccgg atgctgttcc gcaaccaggg
     2041 ctatgccgtc ggcgacatct cgcccgacgg ccgctacctc gccttggtca aggagcggac
     2101 cagcgccgac aacaacctct acctcgccga cctcagcgcc aagtcggcgc cgaagctgat
     2161 caccacgcac aagggcaatg tcagccacgc cgtctacgat ttcagccgcg acggccgcac
     2221 cctcgtctat gcgaccgacg aggccggcga gttcaaccag gcctgggcct acgacctggc
     2281 gagcggcggc aagaagccga tgatcgcggc ggattgggac gtgatgttcg tccaatattc
     2341 gccgtccggc cgctaccgcg tgcacgcgct caacagcgac ggatcgaccg acctcaccat
     2401 caccgcccgc gacggccgcc cggtggcgct gaagggcatc cccgacggcg acgtcggcgg
     2461 cgtccgcttc aaccgcgacg agacgatggt cgccttcacc gtcgcctcgg acacgtcgcc
     2521 cgccgacatc ttcgtcgccg acctcgccac cggccaggcg cggcggctca ccaaggcgct
     2581 aaatccggcg atcgacgagg cgcagctggt cgaagcaacg gtggcccggt tcaagagcta
     2641 tgacgggctg gaagtgcccg gcatcctcta caagccgcgc gaggcgagcg cgtcgaacaa
     2701 ggtcccggct gtggtgttcg tccatggcgg cccgggcggc cagagccggc gcggctattc
     2761 ggcaatggtc cagcacctcg tcaacaatgg ctatgccgtc tacgcgatca acaatcgcgg
     2821 ctcgtccgga tacggcaaga ccttcttcca cctggacgac cgccgccatg gcgacgtcga
     2881 cctcaaggac gtggtcgcct ccaagggctt cctgcagtcg atggactggg tcgacggcga
     2941 gcggatcggc atcatgggcg gctcctatgg cggctatatg gttgccgccg ccttggcgtt
     3001 cgagccgcag gcgttcgacg tcggcgtcaa catcttcggt gtgaccaact gggtgcgcac
     3061 cttgcagtcg atcccgccgt ggtgggaatc gatgcgcgag gctttgttcg acgagatggg
     3121 cgatcccgcc acggacggcg agcggcaccg tgcgatctcg ccgctcttcc acgccaagaa
     3181 tatcgtcaag ccgatgctgg tcgtccaggg cgccaacgac ccgcgggt
//
LOCUS       DASVRK010000239        10392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_236065, whole genome shotgun sequence.
ACCESSION   DASVRK010000239 DASVRK010000000
VERSION     DASVRK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236065"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1589
                     /gene="nusA"
                     /locus_tag="VF589_09385"
     CDS             3..1589
                     /gene="nusA"
                     /locus_tag="VF589_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX8257836.1"
                     /translation="MANSVASEKMIDKGIVIEAMEDAIQRAARARYGAENDIRAKLDA
                     TSGDLRLWRVVEVVEAVDDYFKQVSLEDAQKLQPGAVVGDYIVDPLPPIEFGRIAAQA
                     AKQVIFQKVRDAERERQYEEFKDRAGEVITGVVKRVEFGHVVVDLGRAEGVIRRDQQI
                     PREMVRVGDRIRSLILSVRREARGPQIFLSRAHPDFMKKLFAQEVPEIYDGIIEIKAA
                     ARDPGSRAKIGVISHDSSIDPVGACVGMKGSRVQAVVQEMQGEKIDIIPWSPDLATFV
                     VNALQPATVSRVVIDEEENRIEVVVPDDQLSLAIGRRGQNVRLASQLTTSAIDIMTEA
                     DASEKRQREFIERSELFQNELDVDETLAQLLVAEGFGALEEVAYVELDEIASIEGFDE
                     ELAGELQSRAAEALERREEASREERRALGVEDALAEMPYLNEAMLVTLGKAGIRTLDD
                     LADLATDELVQKKRPEQRRQRESNRPEDKGGILADYGLSDEQGNEIIMAARAHWFEDD
                     DAAASGPADQGEDAVAETSQ"
     gene            1687..2349
                     /locus_tag="VF589_09390"
     CDS             1687..2349
                     /locus_tag="VF589_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HEX8257837.1"
                     /translation="MLSGEHGDRGALIRLALGPDGMVAPDVRAKAPGRGAWIGVDRPT
                     LEAAQAKGKLKGALARAFKTGEASAPADLGEKIEAALRQAALDRLGLEARAGTLLSGS
                     DKIETAARRGQVHLLIHAADAGEDGSRKLDQAWRTGGGGENGAENGLRQGLVFPAERA
                     ILSLALGRQNVVHAALIDRAAAARVRHALDRWRAFIGPNEELSAASAGSPQVSAVDVN
                     EG"
     gene            2351..4885
                     /gene="infB"
                     /locus_tag="VF589_09395"
     CDS             2351..4885
                     /gene="infB"
                     /locus_tag="VF589_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX8257838.1"
                     /translation="MSETTDKPKLGARAPLGLKRTVETGKVKQSFSHGRSNTVVVEVK
                     KRRILGRPGEAPEPAKAAEPAPPPAPVAKALPPAAPAPQPQRPASDTLSRRELQDRLL
                     REAEEARMNALEEARRREDRQAQAATEEERRRAEDNRRAEEEGRVRGEEEARRAAEEA
                     ERAASQPASAEAPAAGAPAVEEDEDRRPAHARPKGFTPVKRPEPARPARGPRDDRRQS
                     GKLTVTRALDDGEGGARARSLAALKRAREKEKRHHQQSGPAAKQVRDVVVPEAITVQE
                     LANRMAERGADLVKALFKLGTPVTVTETIDQDTAELLIEEFGHRMVRVSEADVDIQAN
                     DDVDAPETLQPRPPVVTIMGHVDHGKTSLLDAIRGANVVSGEAGGITQHIGAYQVQLP
                     DKSKITFLDTPGHEAFTEMRARGANVTDLVILVVAADDGLRPQTVEAINHTKAAGVPM
                     IVAINKIDKEGARPQRVREELLQHEIVVEDLGGDVQDVEVSALQKTNLDKLLEAISLQ
                     AEILELKANSSRSAEGAVIEAKLDKGRGPLATVLVQRGTLKVGDIFVVGAISGKVRAM
                     IDDKGRQVKEAGPSVPVEVLGLSGVPSAGDTFTVVENDARAREVAAYRQGVLDRKRTT
                     SAPVSLENMFAAKSDQAVIYPLVVKADVQGSAEAIVNALNRISNEDIKVRILHSGVGA
                     ITESDVTLASASGAPIIGFNVRPNAKAREIAERASVEFQYYDVIYHLTDAVRAAMAGE
                     LGPLMIENVLGRAEVKEVFPAGKKDKAAGLLVLEGILRKGLHARLTREDVIVSKTTIS
                     SLRRFKDDVPEVRAGLECGVLLADTNDIKPGDHLEVFEVEQRERTL"
     gene            4916..5290
                     /locus_tag="VF589_09400"
     CDS             4916..5290
                     /locus_tag="VF589_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8257839.1"
                     /translation="MTVQIVEIAGHKMALLPVAEYERLIDIAEDKADALAAAQAERRR
                     LEGEEYLPAELVDRILGGENPLKVWRKHRGMTLETLADEAGTQPSMLSRIENGKLQGS
                     PGLWRRLAEALKVSADDILPGA"
     gene            5293..5688
                     /gene="rbfA"
                     /locus_tag="VF589_09405"
     CDS             5293..5688
                     /gene="rbfA"
                     /locus_tag="VF589_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX8257840.1"
                     /translation="MASDSEGRSVRLLRVGEQVRHALAEVLMRGDVHDETLASHPVSV
                     TEVRMSPDLRHATVFVKPLLGKDEPVVLKALRTNTAFLQSEVARRVNLKYAAKLKFLA
                     DESFDEGSHIDGLLRSPKVARDLRADEEE"
     gene            5685..6227
                     /locus_tag="VF589_09410"
     CDS             5685..6227
                     /locus_tag="VF589_09410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8257841.1"
                     /translation="MNLAAAMAEGELRLEPLAEAHREPLRAACAEDREIWRIYAISYD
                     PDHFDASFDALLARPNGRGFAIFDGEALVGMSCYIGIEPERGVLEIGNTYYVPKMRGT
                     GLNRRVKELMIGRAVYCDFRRIEFRVDSRNARSQAAMAKLGAVREGVIRAERITWTGH
                     VRDTVLFSILAGEWERVRAR"
     gene            6217..6891
                     /locus_tag="VF589_09415"
     CDS             6217..6891
                     /locus_tag="VF589_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8257842.1"
                     /translation="MPDNVVFRIATWLIPLVFAIVLHEISHGWVANAFGDPTAKRLGR
                     LSPNPIRHVDPVGTVALPLLLAVSGAPVFGWAKPVPVVARKMRNPRLNMMLVALAGPG
                     MNLALALVAAFALALTLAVGGEGPGFRFLAANLENFLAINVFLAVFNLLPIPPFDGGH
                     VVEGLLPRRLARPYRRLGRFGFPLLILLLVVVPLVAPDADLVERVVVPPVRALIEFIA
                     SLAGLG"
     gene            6896..7903
                     /gene="truB"
                     /locus_tag="VF589_09420"
     CDS             6896..7903
                     /gene="truB"
                     /locus_tag="VF589_09420"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX8257843.1"
                     /translation="MHGWLVIDKPVGIGSTQVVGAVKRALREGGYAKVKVGHGGTLDP
                     LASGVLPIALGEATKLSGRMLHADKVYDFTIRFGEETDTLDAEGVAVAFSDVRPALGE
                     VEAVLAAFTGEIEQVPPAYSALKVEGKRAYDLARAGEEVEMKSRRVTVHGLEILPGTG
                     RGTARSAVEGAHRVTGPAEPGPLHHPRLDAGDGPPPRSGEELDQVTLTARVSKGTYIR
                     SLARDIARALGTVGHVTMLRRTRAGPFTLGEAISLDKLSGLAKARQLELAILPLTAGL
                     DDIPALPVTPDQARLLRQGRKLAGIAAQPGLHLATDEHVPVAIVELSEGELRVVRGFN
                     L"
     gene            7919..8188
                     /gene="rpsO"
                     /locus_tag="VF589_09425"
     CDS             7919..8188
                     /gene="rpsO"
                     /locus_tag="VF589_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX8257844.1"
                     /translation="MSVTAERKQEIISDNARGSGDTGSPEVQVAILTERINNLTSHFK
                     THAKDNHSRRGLLMMVNKRRSLLDYIRKEDHERYTALIAKLGLRK"
     gene            8413..>10392
                     /gene="pnp"
                     /locus_tag="VF589_09430"
     CDS             8413..>10392
                     /gene="pnp"
                     /locus_tag="VF589_09430"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX8257845.1"
                     /translation="MFDMKKVEIEWGGKTLTLETGRVARQADGAVLATLGETVVLCAV
                     TAAKNVKEGQDFFPLTVHYQEKYSAAGRIPGGFFKRERGATEKETLTSRLIDRPIRPL
                     FPEGFYNEILVIAHVLSYDGENEPDILAMIAASAALTLSGVPFMGPIAAARVGYQNGE
                     YQLNPSLDEIKEGELDLAVAGTYDAVLMVESEAKELSEDIMLGAVMFAHEQSKPVIDA
                     IIRLAEQAAKEPWELASDENQGEILGKLRELAGTGLAQAYKLVGKAERVAAIAAAKTP
                     FKDAFADADPQTKLKAGKLGKKLEAEIVRTAILKEGRRIDGRDTKTVRPIDAMVGFLP
                     RTHGSALFTRGETQAIVTTTLGTKDAEQMIDGLNGLNYEHFMLHYNFPPYSVGEVGRF
                     GAPGRREVGHGKLAWRAIHPLLPTKDEFPYTIRVLADITESNGSSSMATVCGASLALM
                     DAGVPLKRPVAGIAMGLILEGDEFAVISDILGDEDHLGDMDFKVAGTEAGITTMQMDI
                     KIAGITEEIMRVALDQARDGRAHILAEMAKALDQTRGELSAHAPRIETMQIDKSKIRE
                     VIGTGGKVIREIVAETGAKVDIDDEGLIKISSSDVNQIEAARRWIQGIVEEPEVGKVY
                     TGKVVNLVDFGAFVNFMGGKDGLVHVSEIRNERVEK"
BASE COUNT         1850 a         3466 c         3590 g         1486 t
ORIGIN      
        1 cgatcgcgaa ctcggtcgcg tcggaaaaga tgatcgacaa gggcattgtc atcgaggcga
       61 tggaggacgc gatccagcgc gccgcccgcg ctcgctacgg cgccgagaac gacatccgcg
      121 ccaagctgga cgcgacctcc ggcgaccttc gcctgtggcg ggtcgtcgag gtggtcgagg
      181 cggtcgacga ttatttcaag caggtcagcc tcgaggacgc ccagaagctg cagccggggg
      241 cggtcgtcgg cgattatatc gtcgacccgc tgccgccgat cgagttcggc cggatcgccg
      301 cccaggccgc caagcaggtc atcttccaga aggtccgcga cgccgagcgc gagcgccaat
      361 atgaagaatt caaggaccgc gccggcgagg tcatcaccgg cgtcgtcaag cgcgtcgagt
      421 tcggccacgt cgtcgtcgat ctcggccgcg ccgaaggagt catccgccgc gaccagcaga
      481 tcccgcgcga aatggtccgc gtcggcgacc gcatccgcag cctgatcctg tcggtccgcc
      541 gcgaagcccg cggcccgcag atcttcctgt cgcgggccca ccccgatttc atgaaaaagc
      601 tgttcgccca ggaagtgccc gaaatctacg atggcatcat cgagatcaag gcggccgccc
      661 gcgaccccgg cagccgcgcc aagatcggcg tgatcagcca cgacagctcg atcgatccgg
      721 tcggcgcctg cgtcggcatg aagggcagcc gcgtccaggc agtggtgcag gagatgcagg
      781 gcgagaagat cgacatcatc ccctggtcgc ccgacctcgc caccttcgtc gtcaacgcgc
      841 tgcagccggc cacggtcagc cgcgtcgtca tcgacgagga ggagaaccgg attgaggtgg
      901 tggtgcccga cgaccagctc agcctcgcca tcggccggcg cgggcagaat gtgcggctgg
      961 cgagccagct caccacctcc gcgatcgaca tcatgaccga ggccgacgcc agcgagaaac
     1021 ggcagcgcga gttcatcgag cgctcggagt tgttccagaa cgagctcgac gtcgacgaga
     1081 cgctggcgca gctgctggtc gccgagggct tcggcgcgct cgaggaggtc gcctatgtcg
     1141 agctcgacga gatcgcctcg atcgaagggt tcgacgagga gctggccggc gagctgcaga
     1201 gccgcgccgc cgaagcgctc gagcgccgcg aggaggcctc ccgcgaggag cgccgcgcgc
     1261 tcggcgtcga ggatgcgctg gccgagatgc cgtacctgaa cgaggcgatg ctggtgacgc
     1321 tcggcaaggc cgggatccgg acgctcgacg acctcgccga cctcgccacc gacgagctgg
     1381 tccagaagaa gcggcccgag cagcgccgcc agagggagag caaccggccc gaggacaagg
     1441 gcggcatcct ggccgattat ggcctgtcgg acgagcaggg caacgagatc atcatggcgg
     1501 cccgcgcgca ttggttcgaa gacgatgacg cggccgcgtc ggggcccgcc gatcaagggg
     1561 aggacgcggt tgcggaaacc tcccaatgag cggctgacgg gtcaggcccg aagcaacgcg
     1621 gaagcagaaa ctgggccccg gcattcgccg gggaacagtt cgtcgcacgt ccctgagcgc
     1681 aagtgcatcc tttcgggcga gcatggcgat cgcggcgcgc tgatccgcct ggcgctgggg
     1741 ccggacggga tggtcgcgcc cgacgtgcgc gccaaggcgc ccggccgcgg ggcgtggatc
     1801 ggcgtcgacc ggccgacgct cgaggcggcg caggcgaagg gcaagctcaa gggcgcgctc
     1861 gcccgcgcgt tcaagaccgg cgaggcgagt gcgcccgccg atctcggcga gaagatcgag
     1921 gccgcgctgc ggcaggcggc gctcgaccga ttggggctgg aggcgcgcgc cggaaccctg
     1981 ctgagcggct ccgacaagat cgagaccgcc gcccggcgcg gccaggtcca cctcctcatc
     2041 cacgccgccg atgccggcga ggacggcagc cgcaagctcg accaggcctg gcggaccggc
     2101 gggggcggcg agaacggcgc cgagaacgga ctgcgccaag ggctggtttt cccagcggaa
     2161 agagccatat tgtcattagc gcttgggcgg caaaatgtgg tacatgccgc attgatcgac
     2221 cgggccgccg cagcgcgtgt gcgtcacgcg ctcgatcggt ggcgcgcttt tatcggaccg
     2281 aatgaagagc taagcgccgc ttcggcgggt tcgccgcaag tgtcggctgt ggatgtgaac
     2341 gaaggataat atgagcgaga cgacagacaa gccgaagctc ggagcgcgcg cgccgctggg
     2401 cctgaaacgg accgtcgaga ccggcaaggt gaagcagagc ttcagccatg gccgctcgaa
     2461 cacggtcgtg gtcgaagtga agaagcgccg catcctcggc cgcccgggcg aggcgcccga
     2521 gccggccaag gcagccgagc ccgcgccgcc gcccgcgccg gtcgccaagg cgctgccgcc
     2581 ggccgccccc gcgccgcagc cgcagcgccc ggccagcgat acgctgagcc gccgcgagct
     2641 gcaggaccgc ctgctgcgcg aggccgagga agcgcgcatg aacgcgctcg aggaagcgcg
     2701 ccgccgcgag gatcgccagg cccaggccgc gaccgaggaa gagcgccgcc gcgccgagga
     2761 caatcgccgc gccgaggaag aaggccgcgt ccgcggcgag gaagaagccc gccgagccgc
     2821 cgaggaagcc gaacgcgccg cctcgcagcc ggcctccgcc gaagcgccgg cggccggcgc
     2881 cccagcggtc gaggaagacg aggaccgccg ccccgcgcat gcccggccga agggcttcac
     2941 gccggtcaag cggccggagc ccgcgcgccc ggcgcgcggc ccgcgcgacg atcgccgcca
     3001 gtccggcaag ctcaccgtca cccgcgcgct cgacgatggc gagggcggcg cccgcgcccg
     3061 ttcgctcgcc gcgctgaagc gcgcccgcga aaaggaaaag cgccaccacc agcagtccgg
     3121 ccccgccgcc aagcaggtcc gcgacgtcgt cgtgccggag gcgatcaccg tccaggagct
     3181 cgccaaccgc atggccgagc gcggcgccga cctcgtgaag gcgttgttca agctggggac
     3241 cccggtgacc gtcaccgaga ccatcgacca ggatacggcc gagctgctga tcgaggaatt
     3301 cggccaccgt atggtgcgcg tgtccgaagc cgacgtcgac atccaggcca atgacgatgt
     3361 cgacgcgccg gagacgctgc agccgcggcc gccggtcgtc accatcatgg gccatgtcga
     3421 ccacggcaag acctctttgc tcgacgcgat ccgcggcgcc aacgtcgtct ccggcgaggc
     3481 cggcggcatc acccagcata tcggcgccta tcaggtgcag ctgccggaca agtcgaagat
     3541 caccttcctc gacacgcccg gccacgaagc ctttaccgag atgcgcgcgc gcggcgccaa
     3601 cgtcaccgac ctcgtcatcc tggtcgttgc cgccgatgac ggattgcgcc cgcagacggt
     3661 ggaggcgatc aaccacacca aggcggccgg cgtgccgatg atcgtggcga tcaacaagat
     3721 cgacaaggaa ggcgcgcgtc cgcagcgggt gcgcgaggag ctgctccagc acgagatcgt
     3781 ggtcgaggat ctgggcggcg acgtccagga cgtcgaagtg tccgcgctcc agaagaccaa
     3841 tctcgacaag ttgctcgagg cgatctcgct gcaggccgag attttggagc tcaaggccaa
     3901 ctccagccgc tccgccgaag gcgcggtgat cgaagccaag ctcgacaagg gccgcggccc
     3961 gctcgccacc gtgctggtcc agcgcggcac gctcaaggtc ggcgacatct tcgtggtcgg
     4021 cgccatctcg ggcaaggtcc gcgcgatgat cgacgacaag ggcaggcagg ttaaggaagc
     4081 cggcccttcg gtgccggtcg aagtgctcgg cctgtcgggc gtaccgtccg cgggcgacac
     4141 cttcacggtg gtcgagaacg acgcccgcgc ccgcgaggtc gcggcctatc gccagggcgt
     4201 gctcgaccgc aagcgcacca ccagcgcgcc ggtcagcctc gagaatatgt tcgcggccaa
     4261 gtccgaccag gcggtgatct atccgctggt cgtcaaggcc gacgtccagg gctcggccga
     4321 ggcgatcgtc aatgcgctca accgaatctc gaacgaggac atcaaggtcc gcatcctcca
     4381 ttcgggggtc ggcgccatca ccgagagcga cgtcactttg gcgagcgcgt cgggagcgcc
     4441 gatcatcggc ttcaacgtcc ggcccaacgc caaggcgcgc gagatcgccg agcgggcgag
     4501 cgtcgagttc caatattatg acgtcatcta ccacctcacc gacgcggtgc gggcggcgat
     4561 ggcgggcgag ctcgggccgc tgatgatcga gaacgtgctc ggccgcgccg aggtcaagga
     4621 ggtcttcccg gccggcaaga aggacaaggc ggccggcctg ctggtgctcg aaggcatctt
     4681 gcgcaagggg ctccatgccc gtttgacgcg cgaggacgtc atcgtttcga agaccacgat
     4741 ctcgtcgctg cggcgcttca aggacgatgt ccccgaagtg cgcgccggcc tcgaatgcgg
     4801 cgtgctcctg gcggacacga acgacatcaa gccgggcgac catctcgaag tcttcgaagt
     4861 cgagcaacgc gagcgcacgc tctaaggctt gggcgagtag cgagtgtcct acggcatgac
     4921 tgtgcagatc gtggaaattg caggtcacaa gatggcgctg ctccccgtcg ctgaatatga
     4981 gcggctgatc gatatcgccg aggacaaggc ggatgcgttg gcggcggcgc aggcggagcg
     5041 gcggcggctg gagggcgagg aatatctccc cgccgagttg gtcgatcgca tccttggtgg
     5101 tgagaacccg ctcaaagtct ggcggaagca tcgcggcatg acgctcgaaa ccctggcgga
     5161 cgaagccggg acccagcctt cgatgctttc gaggatcgaa aatggcaagc tacagggatc
     5221 gccggggctg tggcggaggc tcgccgaagc gctgaaggtc tccgccgacg acatcctgcc
     5281 gggcgcctga cgatggcttc cgattcagaa ggccgctccg ttcgcttgct ccgcgttggc
     5341 gagcaggtgc gccatgcttt ggccgaggtg ctgatgcggg gcgacgtcca tgacgagacg
     5401 ctggctagcc acccggtctc ggtcaccgag gtgcggatgt cgccggacct gcgccacgcg
     5461 accgtgttcg tgaagccttt gctcggtaag gacgagccgg tcgtgctgaa ggcgctgcgc
     5521 accaacaccg cctttttgca gagcgaagtg gcgcggcggg tgaacctcaa atatgcggcc
     5581 aagctcaagt tcctggccga cgagagcttc gacgaaggca gccatatcga cggcctgctg
     5641 cgctcgccca aggtggcgcg cgacctccgc gccgacgagg aggaatgaac ctcgccgccg
     5701 cgatggccga gggcgagcta cggctcgagc ccttggccga ggcgcatcgc gagccgctga
     5761 gggcggcttg cgccgaggat cgggagatct ggcggatcta cgccatctcc tacgatcccg
     5821 accatttcga cgcgagcttc gatgccttgc tggcgcggcc gaacgggcgc ggcttcgcga
     5881 tcttcgatgg cgaggcgctg gtcgggatga gctgctatat cggcatcgag ccggagcggg
     5941 gcgtgctcga gatcggcaac acttattacg tcccgaagat gcgcggcacc ggcctcaacc
     6001 ggcgcgtgaa ggagctgatg atcggccgcg cggtctactg cgacttccgc cggatcgaat
     6061 tccgggtcga cagccgcaac gcccgctcgc aggcggcgat ggcgaagctc ggcgcggtgc
     6121 gggaaggcgt gatccgggcc gagcggatca cctggaccgg gcatgtccgc gataccgtgc
     6181 tgttctcgat cctggccggc gaatgggagc gcgtccgtgc ccgataatgt cgtgttccgg
     6241 atcgccacct ggctgatccc gttggtgttc gcgatcgtcc tccacgaaat ctcgcacggc
     6301 tgggtcgcca acgccttcgg cgaccccacc gccaagcggc tcgggcggct cagccccaac
     6361 ccgatccgcc atgtcgatcc ggtcgggacg gtcgcgctgc cgctgctgct ggcggtgagc
     6421 ggcgccccgg tgttcggctg ggcgaagccg gtgccggtgg tggcgcgcaa gatgcgcaac
     6481 ccgcggctca acatgatgct ggtggcgctg gctgggcctg ggatgaacct ggcgctggcg
     6541 ctcgtcgccg ccttcgctct ggcgctcacg ctcgccgtcg gcggcgaagg gccgggcttc
     6601 cgcttcctcg ccgccaacct tgagaatttc ctggcgatca acgtgttcct cgcggtgttc
     6661 aacctgctgc cgatcccgcc gttcgacggc ggccacgtcg tcgaagggct gctgccgcgg
     6721 cggctggcgc ggccgtatcg ccggctcggc cggttcggct ttccattgct gatcctgctg
     6781 ctggtggtgg tgccgctggt cgcccccgac gccgacctgg tcgagcgggt ggtggtgccg
     6841 ccggtgcggg cgctgatcga attcattgcc tcgctcgccg gcctgggctg aggcgatgca
     6901 cggctggctg gtgatcgaca agccagtcgg catcggctcc acgcaagtgg tcggggccgt
     6961 gaaacgcgcg ctgcgcgagg gcggctatgc caaggtcaag gtcggccatg gcggcacgct
     7021 cgacccgctg gcgagcggcg tgctgccgat cgcgctcggc gaggcgacca agctcagcgg
     7081 gcgcatgctc catgccgaca aggtgtacga cttcaccatc cgcttcggcg aggagaccga
     7141 cacgctcgat gccgaggggg tggcggtggc cttctccgac gtgcggccgg ccttgggcga
     7201 ggtggaggcg gtgctggcgg cgttcacggg cgagatcgag caggtgccgc cggcttattc
     7261 ggcgctcaag gtggagggga agcgggctta cgatctggcg cgggccggcg aggaggtcga
     7321 gatgaagagc cggcgggtga cggtgcacgg gctggaaatc ctccccggaa cggggagggg
     7381 gaccgcacga agtgcggtgg agggggccca ccgcgtcacc ggtccggcgg agccgggccc
     7441 cctccaccat ccccggctgg acgccgggga tggtccccct ccccgttccg gggaggagct
     7501 ggatcaggtg acgctgaccg cgcgcgtctc gaagggcacc tacatccggt cgctggcgcg
     7561 ggacattgcg cgcgcgctcg gcacggtcgg ccacgtcacc atgctgcggc ggaccagggc
     7621 cgggccgttc accctcggcg aggcgatttc gctggacaaa ttgagcgggc tcgctaaggc
     7681 ccggcagctt gaactagcaa tattgccgct gacggcgggg ctggacgaca tcccggctct
     7741 acccgtcacc cccgaccagg caaggctgct ccgacagggg cggaaattgg ccgggatcgc
     7801 cgcacagccg gggctccacc ttgcaaccga cgaacatgtt ccggttgcga tcgtggagct
     7861 tagcgaaggg gaacttcgcg tggtgcgcgg cttcaaccta tagaataagg agtccccgat
     7921 gtcggtcact gccgaacgca agcaagagat tatcagcgac aacgcccgcg gatcgggcga
     7981 caccggatcg cccgaggtcc aggtcgccat cctcaccgag cgcatcaata acctcaccag
     8041 ccacttcaag acgcacgcga aggacaatca ttcccgccgc ggcctcctga tgatggtcaa
     8101 caagcgccgc tccctgctgg attacatccg caaggaagat cacgagcgct acaccgcact
     8161 catcgcgaag ctcggccttc gcaagtgacg caatcgttcg gcccgcctcg gcgggccgat
     8221 tacgtattgc gtcatcccgg cggaggccgg gatccagact gggctcctgc tttcgcagga
     8281 gcacaatagc cagactgggc tcctgctttc gcaggagcac aatagccagc aagcaatccc
     8341 gcgaggctgg caggaccaga caggtccaca caggccccgg ccgcatcggg cggccggacg
     8401 gaaggaaatc aaatgttcga tatgaagaaa gtagaaatcg agtggggcgg aaaaaccctg
     8461 acgctcgaaa ccgggcgcgt tgcccgccag gccgatggcg ccgtgctcgc caccttgggc
     8521 gagaccgtcg tcctttgcgc cgtcaccgcc gccaagaatg tcaaggaagg gcaggacttc
     8581 ttcccgctga ccgtccacta tcaggaaaaa tattcggccg ccgggcgcat cccgggcggc
     8641 ttcttcaagc gcgagcgcgg cgccaccgaa aaggaaacgc tgaccagccg cctgattgac
     8701 cggccgatcc ggccgctctt ccccgaaggc ttctacaacg agatcctcgt gatcgcgcat
     8761 gtgctgagct acgatggcga gaacgagccc gacatcctgg cgatgatcgc cgcgtcggcg
     8821 gccctgaccc tgtcgggcgt ccccttcatg ggcccgatcg ccgccgcccg cgtcggctat
     8881 cagaatggcg aatatcagct caacccgtcg ctcgacgaga tcaaggaagg cgaactggac
     8941 ctcgcggtcg ccggcaccta cgacgccgtg ctgatggtcg aatcggaagc caaggagctt
     9001 tcggaggaca tcatgctcgg cgccgtcatg ttcgcgcacg agcagtccaa gccggtgatc
     9061 gacgccatca tccgcctcgc cgagcaggcc gccaaggagc cgtgggagct cgccagcgat
     9121 gaaaatcagg gcgagatcct cggcaagctg cgcgaactcg ccggcaccgg gctcgcacag
     9181 gcctataagc tggtcggcaa ggccgagcgc gtcgccgcca tcgccgccgc caagacgccc
     9241 ttcaaggacg cctttgccga cgccgatccg cagaccaagc tcaaggccgg caagctcggc
     9301 aagaagcttg aagccgagat cgtccgtacc gcgatcctga aggaaggccg ccgcatcgac
     9361 ggccgcgaca ccaagaccgt gcgtccgatc gacgccatgg tcggcttcct gccgcgcacc
     9421 cacggctcgg cgctgttcac gcgcggcgag acccaggcga tcgtcaccac cacgctcggc
     9481 accaaggacg ccgagcagat gatcgacggt ttgaatggcc tcaattacga gcatttcatg
     9541 ctccactata acttcccgcc ttattcggtc ggtgaagtcg gccgcttcgg cgctccgggg
     9601 cggcgcgagg tcggtcacgg caagctcgcc tggcgggcga tccacccgct gctgccgacc
     9661 aaggacgagt tcccctatac gatccgcgtg ctggccgaca tcaccgagtc gaacggctcc
     9721 tcgtcgatgg cgaccgtctg cggcgcctcg ctggcgctga tggatgcggg cgttccgctg
     9781 aagcggccgg tggcaggcat cgccatgggc cttatcctcg aaggcgacga gttcgcggtc
     9841 atctccgaca tcctcggcga cgaggaccat ctcggcgaca tggacttcaa ggtggcgggc
     9901 acggaagccg gcatcaccac gatgcagatg gacatcaaga tcgcgggcat caccgaagag
     9961 atcatgcgcg tcgcgctcga tcaggcgcgt gacggccgcg cgcacatcct ggctgaaatg
    10021 gccaaggcgc tcgaccagac ccgcggcgag ctttcggcgc acgcgccgcg catcgagacg
    10081 atgcagatcg acaagtcgaa gatccgcgaa gtcatcggca ccggcggcaa ggtcatccgc
    10141 gagatcgtcg cggagaccgg cgccaaggtc gacatcgacg atgagggcct gatcaagatc
    10201 agctcgtccg atgtcaacca gatcgaagcg gcgcgccgct ggatccaggg cattgtcgag
    10261 gagcccgagg tgggcaaggt ctacaccggc aaggtcgtca acctggtcga tttcggcgcc
    10321 ttcgtgaact tcatgggcgg caaggacgga ctcgtccacg tctccgagat ccgcaacgag
    10381 cgggtcgaga ag
//
LOCUS       DASVRK010000240         7499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_482945, whole genome shotgun sequence.
ACCESSION   DASVRK010000240 DASVRK010000000
VERSION     DASVRK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482945"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VF589_09435"
     CDS             complement(<1..731)
                     /locus_tag="VF589_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257846.1"
                     /translation="MTNPSPTPRPPAPPAAEPPASAPFTPVELRARHDGWTPTRQREF
                     VEALAICGCVVEAAARTGMTEQSAYRLRLRPDAGPFAAAWDAALVVGKTPLASIAWDR
                     AINGTATPIFYKGEQVGERRHYSDKLLLHLLGNTELTGRRAAARDAVLADWDGWMEGL
                     EHGFPQPEPQPEPEPEPDGPPQRIITGNYWMEDGIWYTDKTPAPGYTGTIIGGCITRR
                     RTLDEKELAEAAWQLEEWEQEILKE"
     gene            complement(932..1339)
                     /locus_tag="VF589_09440"
     CDS             complement(932..1339)
                     /locus_tag="VF589_09440"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8257847.1"
                     /translation="MDETRADGPAAAPAGEAREGVRRLISARIDDTNVYDTDGGKIGT
                     LYSVHIDRWSGQIEYAVLSFGGFLGLGQSYHPVPFQMLSVNEDRGGYTVHLGRSVLEG
                     GPSYRPDTAPPWDSTYARRISDYYGIPPRSGTF"
     gene            complement(1367..1924)
                     /locus_tag="VF589_09445"
     CDS             complement(1367..1924)
                     /locus_tag="VF589_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HEX8257848.1"
                     /translation="MPAAFRRARSAEAAASVRKRTPACPPPDRSRNGSPPGDVSLAPG
                     NSGKRHPLKPLDSIFAGLARSRFRSRFRLGPRERAYLAAKGPETIRAHAADFIAARLA
                     PAEPANDGRQTPMRGHPIFIAQHATATCCRACLAKWHNIPAGRALTPAEQQHVLAALD
                     RWLAAQPAPEPLGEPPAGTTRPALG"
     gene            1963..2535
                     /locus_tag="VF589_09450"
     CDS             1963..2535
                     /locus_tag="VF589_09450"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX8257849.1"
                     /translation="MNMLSRILTAAALLAVAAPAAAQEVVPLGRFDSIQLNGGGSVRL
                     RHGPVQRVTLIRGSTRYSHVRVEGRERELVIDACNGRCPRRYDLEIEIVTPDVAAVAV
                     NGGGEIVVASGFPRQESVAAAVNGGGAIDLRALPVREAAASVHGGGSLKVRPAASLAA
                     SVMGGGEIRYWGNPEVASSINGGGLVRRGD"
     gene            complement(2706..3404)
                     /locus_tag="VF589_09455"
     CDS             complement(2706..3404)
                     /locus_tag="VF589_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8257850.1"
                     /translation="MIDIRPFGTLGQADHGWLNARHHFSFANYHEPARMGWGAIRVWN
                     DDEIAAKSGFPPHPHSDMEIITYVRQGAITHQDSMGNKGRTGAGDVQVMSAGTGVRHA
                     EYNLEDEQTNLFQIWVETDQRGAEPSWGAREFPKHERDGSFVTLASGFADDAEALRIN
                     AAARVMGATLKAGEAAELTLDPARHVYLVAVNGPVEVNGVRAEARDGVAVTGEATLNI
                     RALDETEIVLVDAR"
     gene            complement(3572..4201)
                     /locus_tag="VF589_09460"
     CDS             complement(3572..4201)
                     /locus_tag="VF589_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEX8257851.1"
                     /translation="MTKVLIIDSAATGEASVSHKLTDALAERLSRRDPSVHIVRRDIG
                     AAPIPHLTAETVGVIRGAPAETDAARQAAALSDALVAELKEADLIVIGAPMYNFGMAS
                     TLKAWFDHVLRAGITFRYSEAGPEGLVKGKRAVVIEARAGVYSEGPAAAMDHQEPHIR
                     TLLGFMGVTDDEFVRAEKLAFGPDAAAAAIAEAVDRLDGFARTELALAA"
     gene            4328..5266
                     /locus_tag="VF589_09465"
     CDS             4328..5266
                     /locus_tag="VF589_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8257852.1"
                     /translation="MERSGVDLMDVLAFVRVVETGAFARAAERMDLSKSILSRRVARL
                     EDQLGARLLTRSAQGAQPTDIGEAYYARAANVLAELEAAQEVVADAVTQVAGPLRITA
                     PLSFGSQHLAPALAEFAKLHPRVEFDVSLNDRTVDLIGGGYDMAVRIGNLPDSSLVAR
                     RIAPVRAAVLASPGYIAERGRPLHPRDLADHDLLFYANIPAYEQWRFRVGNHWEHVKG
                     HARFRADNGEMLREAACAGLGIVVLPSFIASGSIREGKLEVLLRDYPFEEIGLHLVMP
                     PGRASTARVRALIDFLAARFGPEPSWDPCWMADGGC"
     gene            complement(5290..5910)
                     /locus_tag="VF589_09470"
     CDS             complement(5290..5910)
                     /locus_tag="VF589_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX8257853.1"
                     /translation="MGGIKAKALKASIDALAGREPLFAAALACSGYPEPRVNPPGYPT
                     LLRTIIGQQVSLASARAIWTKLEAALGEAERPESMAAASDEALRGAGLSRQKMAYARS
                     LADEILSGRLDLQNLAEDDEEAIAELVRVKGIGRWSAEIYLLFAEGRPDIWPAGDLAI
                     QVAVGRILELPERPTEKQVRDLAEPWRPHRGAAAIFAWHHYKTPVL"
     gene            complement(5927..6673)
                     /gene="rlmB"
                     /locus_tag="VF589_09475"
     CDS             complement(5927..6673)
                     /gene="rlmB"
                     /locus_tag="VF589_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX8257854.1"
                     /translation="MRRKARQAAKPDNRPRFWGRHAVAAALANPERRIVRISVTREAA
                     AEFDFAPGVPVTYADAADLGRLVPRDAPHQGIVAEVERLPDRLLADLLDEAEDGRPLL
                     VLDQVTDPHNVGAILRSAAAFDALGIVTQDRHAPAESGALAKAASGALETMPWVRVVN
                     LARALDEMAQAGFWRVGLTGEADTSLAEAMGPPRIALVLGAEGEGMRQNTEAHCDALA
                     RLPISDRVESLNVSNAAAVALYAVRVASAS"
     gene            6764..7498
                     /locus_tag="VF589_09480"
     CDS             6764..7498
                     /locus_tag="VF589_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257855.1"
                     /translation="MATGYIASEARPAGRLFYSAMGLLIAAIVFAGFARTYYLSHWLT
                     PPARTPEITPLLHLHALVFTGWILLQAAQPALVARGRVGLHRALGYGGAGLAFAVWLL
                     GNSAAVEAIEAGYKGVGDPYAFYAITFFSIQAFGIIVALGIARRRDAEAHKRLMLLSS
                     AAILEAAVGRLPLDLVEATAPLSFYLGADLVIAAGILHDLAVRGRVHAVWAWGGGLLV
                     ASQILRVAIMDTGPWLAFARAMAALV"
BASE COUNT          989 a         2376 c         2913 g         1221 t
ORIGIN      
        1 tcctccttga ggatctcctg ctcccactcc tcgagctgcc aggcggcctc ggcgagctcc
       61 ttctcgtcca gcgtccgccg gcgggtgatg cagccgccga tgatcgtgcc ggtatagccc
      121 ggcgccggcg tcttgtcggt gtaccagatg ccgtcttcca tccagtagtt gccggtgata
      181 attcgttgcg gcggcccgtc cggctccggc tccggctccg gttggggttc cggttgggga
      241 aaaccatgtt cgaggccttc catccagccg tcccaatcgg cgagcacggc gtcgcgggcg
      301 gcggcgcggc ggccggtcaa ttcggtattg ccgagcaggt ggagcagcag cttgtcgcta
      361 taatggcggc gctcgccgac ctgttcgcct ttgtagaaga tcggcgtcgc ggtgccgttg
      421 atggcgcggt cccaagcgat cgacgccagc ggagtcttgc cgaccaccag ggccgcgtcc
      481 caggcggcgg cgaagggtcc cgcgtccggc cggagacgga gccggtaggc gctctgctcg
      541 gtcatgccgg tccgggccgc ggcttcgacg acgcagccgc aaattgcgag cgcctcgacg
      601 aactcgcgct ggcgggtggg cgtccagcca tcgtggcggg cgcggagttc gacgggggtg
      661 aagggggcgg aagcgggagg ctccgcggcg gggggcgccg gtgggcgcgg ggtggggctt
      721 gggttcgtca tagccgagac agcagaccat attggttctc tgtaggaaag ttcttttttt
      781 gttctgagga ggggtcgcat tcctcccctg cctttgcagg ggagggggac cgcccgtagg
      841 gcggtggagg ggcttcttca ggcggggtga ggcgctacgt tcctcctcgc gaaagtagag
      901 gcccctcccc caagctgcgc tgggggagcg tctagaacgt gccgctgcgg ggggggatgc
      961 cgtaataatc ggagatgcgg cgggcgtagg tggaatccca aggcggggcg gtgtcggggc
     1021 ggtagctggg gccgccttcc agcaccgagc ggccgagatg gacggtgtag ccgccgcggt
     1081 cctcgttgac gctcagcatc tggaatggga ccggatggta gctctggccg aggccgagga
     1141 agccgccgaa cgacagcacc gcatattcga tctggccgga ccagcggtcg atatggacgc
     1201 tgtagagagt gccgatcttg ccgccgtctg tgtcgtagac gttggtgtcg tcgatccgcg
     1261 ccgaaatgag gcgcctgacg ccctcgcggg cctcgccggc aggggcggcg gccgggccgt
     1321 ccgctcgcgt ctcgtccatc gcttgctcct ctcgccaatc gcggtttcaa ccgagggcag
     1381 ggcgggtggt tccggcgggc ggttcgccaa gcggttcagg cgccggttgg gcggcgagcc
     1441 agcggtcgag ggcggcgagg acatgctgct gctcggcggg ggtgagcgcg cggccggcgg
     1501 ggatgttgtg ccatttggcg aggcaggcgc ggcagcaggt ggcggtggcg tgctgggcga
     1561 tgaagatggg gtggccgcgc atcggggtct ggcggccgtc attggcgggt tcggccggag
     1621 cgagccgggc ggcgatgaag tcggcggcgt gggcgcggat cgtctccggt cccttagcgg
     1681 cgagataggc gcgctcgcgc gggccgaggc ggaagcggct gcggaagcgc gagcgggcga
     1741 ggccggcgaa gatcgaatcg agcggtttca gcgggtggcg ctttcccgag tttcctggtg
     1801 cgagggaaac gtcgcctggc gggctgccgt tccggctcct gtccggcggc ggacaggcag
     1861 gagtccgctt ccggacagac gccgcggctt ccgcgctccg cgcgcggcgg aaggcggctg
     1921 gcacggctct tgctgcgaaa ggaggcgaag ctgaacaggg agatgaacat gctatcgagg
     1981 attttgacgg cggcggcgct gctcgcggtc gcggctccgg cggcggcgca ggaggtggtg
     2041 ccgctgggcc ggttcgattc gatccagctc aacggcggcg gcagcgtcag gctgcgccat
     2101 ggcccggtcc agcgggtgac tttgatccgg ggcagcaccc gctattcgca tgtccgcgtc
     2161 gagggccgcg agcgggaatt ggtgatcgac gcctgcaacg gccgctgccc gcggcgctac
     2221 gacctcgaga tcgagatcgt gacgcccgac gtggctgcgg tggcggtgaa cgggggcggc
     2281 gagatcgtgg tggcgagcgg ctttccgcgg caggaatcgg tggcggcggc ggtcaatggc
     2341 ggcggcgcga tcgacctgcg cgcgctgccg gtgcgcgagg cggcggcgtc ggtgcatggc
     2401 ggcggcagcc tcaaggtgcg gccggccgcc agcctcgccg cctcggtgat gggcggcggc
     2461 gagatccgct attggggcaa tcccgaagtc gccagctcga tcaacggcgg cggcctggtg
     2521 cggcgggggg actgagcggc gccgggcggc cgggtggtcg gctaaagctt tttcgggctg
     2581 acgaggcgct gctccccggc gaaagccggg gtccagctgc gctggagtct ggaccccggc
     2641 cttcgccggg gaacagtccg gaatacgctt tgaagggcgg cgccgggtgg cgccgccctc
     2701 ccggattagc gggcgtcgac gaggacgatc tcggtctcgt cgagggcgcg gatattgagc
     2761 gtcgcctcgc cggtcacggc gacgccgtcg cgggcctcgg cgcggacgcc gttgacctcg
     2821 accgggccgt tgaccgcgac cagatagacg tgccgggccg gatcgagcgt gagctcggcg
     2881 gcctcgcccg ccttcagcgt cgcgcccatc acgcgggcgg cggcgttgat cctgagcgcc
     2941 tcggcatcgt cggcaaagcc gctcgctaaa gtgacgaagc tgccgtcgcg ctcatgcttc
     3001 gggaattcac gggcgcccca gctcggctcg gcgccgcgct ggtcggtctc gacccagatc
     3061 tggaacaaat tggtctgctc gtcctcgaga ttatattcgg cgtggcggac gccggtccct
     3121 gcggacatca cctggacgtc gccggcgccg gtgcggccct tattgcccat cgaatcctga
     3181 tgggtgatcg cgccctggcg gacataggtg atgatctcca tgtccgaatg cgggtgcggc
     3241 gggaagccgg acttggcggc gatctcgtcg tcgttccaga cccggatcgc gccccagccc
     3301 atgcgggcgg gctcgtgata attggcgaag gagaaatggt ggcgggcgtt gagccagcca
     3361 tggtcggcct ggccgagcgt gccgaaggga cggatgtcga tcatgggatt tcctccgaaa
     3421 acccgccgga gcttgggctc cggcgggcga attgcctgga atgtggggcc ttgccggccc
     3481 ggtctcaacg ggggagtcac aacgggtgat ccgcccgccc cgcccgaccg ggggcatagc
     3541 cgggcgaggc ggacggatcg ggcgccggcg gttacgcggc cagcgccaat tcggtgcggg
     3601 cgaagccgtc gagccggtcg accgcctcgg cgatcgccgc cgcggcggcg tcgggaccga
     3661 aggccagctt ctcggcgcgg acgaattcgt cgtcggtgac gcccatgaag ccgagcaggg
     3721 tgcggatgtg cggctcctga tggtccatcg ccgcggccgg gccctcgcta tagacgcccg
     3781 cgcgggcctc gatcaccacc gcgcgcttgc ccttgaccag gccctcgggg cccgcctcgg
     3841 aatagcggaa ggtgatgccg gcgcgcagga catggtcgaa ccaggccttg agcgtcgaag
     3901 ccatgccgaa attgtacatc ggcgccccga tcacgatcag gtcggcttcc ttgagctcgg
     3961 cgaccaaagc gtccgaaagc gcggcggctt ggcgggcggc gtcggtctcg gcgggggcgc
     4021 cgcggatgac gccgacggtc tcggcggtga ggtgcgggat cggcgcggcg ccgatgtcgc
     4081 ggcggacgat gtgcaccgaa gggtcgcgcc ggctcagccg ttcggcgagc gcgtcggtga
     4141 gcttgtgcga caccgaggcc tcgccggtgg cggcgctgtc gatgatgaga accttggtca
     4201 tgttgcctcc tgaacctccg gcttcacggg ccgggatctg cttgctgaaa gccagataag
     4261 cgcgtagcag cgttgcgagt agccgttcgc ggcgcattgt ggtgttgcaa tatatggaac
     4321 gctgaatatg gaacgctcgg gggtcgacct gatggatgtg ctcgccttcg tgcgggtggt
     4381 ggagaccggc gccttcgccc gcgccgccga acggatggac ctgtcgaaat cgatcctaag
     4441 ccggcgggtg gcgcggctcg aggatcaatt gggcgcgcgg ctgctgacgc gcagcgcgca
     4501 gggcgcgcag ccgaccgaca ttggcgaggc ttattatgcg cgcgcggcca acgtgctggc
     4561 cgagctcgaa gcggcgcagg aggtggtggc ggacgcggtg acgcaggtgg cggggccgct
     4621 gcggatcacc gcgcccttgt cgttcggaag ccagcatctc gcgcccgcgc tggccgagtt
     4681 cgccaagctc caccccaggg tggaattcga cgtgtcgctg aacgaccgca cggtcgacct
     4741 gatcggcggc ggctacgaca tggcggtcag gatcggcaat ttgccggatt cctcgctggt
     4801 ggcgcgccgc atcgcgccgg tgcgggcggc ggtgctggcc agccccggct atatcgccga
     4861 gcgcggccgg ccgctccacc cgcgcgacct ggccgaccat gacctgctct tctacgccaa
     4921 catcccggct tatgagcaat ggcggttccg ggtcggcaac cattgggagc atgtgaaggg
     4981 ccatgcccgc ttccgcgccg acaatggcga gatgctgcgc gaggcggcct gcgccgggct
     5041 cggcatcgtc gtgctgccga gcttcatcgc ctcgggttcg atccgcgagg gcaagctcga
     5101 agtgctgctg cgggattatc cgttcgagga gatcgggctg cacctggtga tgccgccggg
     5161 ccgcgcctcg accgcgcggg tgagggcgct gatcgatttc ctcgccgccc gcttcggccc
     5221 ggagccgagc tgggacccgt gctggatggc ggacgggggg tgttgacccc gtcgaggtag
     5281 ctggatgcgc taaaggaccg gcgtcttgta atggtgccag gcgaagatgg cggcggcgcc
     5341 gcggtggggg cgccagggtt cggcgaggtc gcggacctgc ttttcggtgg ggcgttcggg
     5401 gagctccaag atgcggccta ccgctacctg gatggcgagg tcgccggccg gccagatgtc
     5461 ggggcggcct tcggcgaaca gcaaatagat ttccgccgac cagcggccga tccccttgac
     5521 gcggacgagc tcggcgatcg cctcctcgtc gtcctcggcg agattttgaa ggtcgagccg
     5581 gccgctgagg atttcgtcgg cgaggctgcg ggcataggcc atcttctggc gcgagaggcc
     5641 ggcgccgcgc agcgcctcgt cgctggccgc ggccatgctc tcgggacgct cggcctcgcc
     5701 gagcgccgct tcgagcttgg tccagatcgc gcgcgcgctg gcgaggctga cctgctggcc
     5761 gatgatggtc ctgagcaaag tcgggtagcc gggcgggttg acccgcggct cgggatagcc
     5821 cgagcaagcg agggcggcgg cgaacagcgg ctcgcgcccg gctagcgcgt cgatcgaggc
     5881 tttgagcgcc ttcgccttta tgccgcccat ggccgcgccc cggagctcat gaagcggagg
     5941 caacccgcac cgcgtaaagc gccaccgccg ccgcgttcga gacgttgagg ctttccaccc
     6001 ggtcgctgat cggcaggcgc gccagcgcgt cgcaatgggc ctcggtattc tggcgcatgc
     6061 cctcgccctc ggcgcccagc accaaggcga tgcgcggcgg ccccatcgct tcggcgaggc
     6121 tcgtgtcggc ctcgccggtg aggccgaccc gccagaagcc ggcctgcgcc atctcgtcga
     6181 gcgcgcgggc gaggttcacc acccgcaccc acggcatggt ctcgagcgcg ccgctggcgg
     6241 ccttggcgag cgcgcccgat tcggcgggcg cgtggcggtc ctgggtgacg atgccgagcg
     6301 cgtcgaacgc ggcggccgag cgcaggatgg cgccgacatt gtgcggatcg gtgacctggt
     6361 cgagcaccag caaaggccgg ccgtcctcgg cctcgtcgag gagatcggcc agcagccggt
     6421 cgggcaggcg ctcgacctcg gcgacgatgc cctgatgggg cgcgtcgcgc ggcaccagcc
     6481 ggccgaggtc ggcggcgtcg gcgtaggtga ccggcacgcc gggcgcgaag tcgaactcgg
     6541 cggcggcctc gcgcgtgacc gagatgcgga cgatccggcg ctcgggattg gcgagcgcgg
     6601 cggcgaccgc gtggcggccc cagaagcggg ggcggttgtc gggcttggcg gcctggcggg
     6661 ctttgcggcg catggcgcgt gcatggcgtt ccgcgtgcgg gctggcaagc gggtctctga
     6721 accggcggcg cgcgcgggcg ttcggggcag gaagggaggc aggatggcga ccggttatat
     6781 tgcgagcgag gcgcggccgg cggggcggct tttctacagc gcgatggggc tgctcatcgc
     6841 ggcgatcgtg ttcgccggtt tcgcacgcac ttattatctg tcgcactggc tgacgccgcc
     6901 ggcgcggacg cccgagatca cgccattgct ccaccttcac gccttggtgt tcaccggctg
     6961 gatattgctt caggcggcgc agccggcgct ggtcgcgcgg gggagggtgg ggctgcaccg
     7021 cgcgctcggc tatggcggcg ccggccttgc cttcgccgtg tggctgctcg gcaattccgc
     7081 cgcggtcgag gcgatcgagg ccggctacaa gggcgtcggc gacccttatg ccttttacgc
     7141 gatcaccttc ttctcgatcc aggcgttcgg gatcatcgtc gcgctcggca tcgcccggcg
     7201 ccgcgatgcc gaggcgcata aaaggctgat gctgctgtcc agcgccgcga tcctggaggc
     7261 ggcggtcggc cggctgccgc tcgacctggt ggaggcgacg gcgccgcttt ccttctatct
     7321 gggcgccgac ctcgtcatcg ccgccggcat ccttcacgac ctcgcggtgc ggggccgggt
     7381 gcatgcggtg tgggcgtggg gcggcgggct gctggtggcg agccagatct tgcgggtggc
     7441 gatcatggat acggggccgt ggctggcctt cgcccgggcg atggcggcgc tcgtctgag
//
LOCUS       DASVRK010000241        24620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_110151, whole genome shotgun sequence.
ACCESSION   DASVRK010000241 DASVRK010000000
VERSION     DASVRK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 24620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24620
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110151"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VF589_09485"
     CDS             <1..192
                     /locus_tag="VF589_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8257856.1"
                     /translation="PLLDGGHLLFYTIEGVRRKPLRPEAQEWAFRTGLAALLALMIFV
                     TLNDLASFGVWTRLGGLIG"
     gene            328..3051
                     /gene="bamA"
                     /locus_tag="VF589_09490"
     CDS             328..3051
                     /gene="bamA"
                     /locus_tag="VF589_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX8257857.1"
                     /translation="MGTMLGGFAVPAHAQDAQAAPEASAPAQDPAAPVAPPAATPADA
                     QAPVAGGGVPAAAAPIVAPAPTGTVRSITVTGNQRLETETVVSYMPLRVGEPYTREAL
                     DEALRDLYATELFADVQIRDNLGALTVEVRENPVINRIILEGNKRIKDAKIAPEIRLA
                     PRQIFTRSKVRADVARIIELYRRQGRFAANVEPKIVQLEQNRVDLVFEINEGPRSKVR
                     SINILGNARFSDGRLRSEMETRQTSLLSFLGSADTYDPDRLAYDQQKLRQFYLTEGYA
                     DFRVVSAVAELTPDRRDFIITYVVEEGDRYKFGDVKVTSGIRDFNPETLAKGLPMKTG
                     DWYDAKEIEDTVTSLTETAGVFGYAFAQVQPQFERNKETLTMSVNFQIAEAPRVYVER
                     IDINGNTVTRDKVIRREFRLAEGDPFNSIQVKRSRDRIQSLGFFQDNLEIEQQPGSTP
                     DRVVLAVDVEEKSTGDIQLSAGFSSLERFLVNASIRQRNFMGKGQEVRAAINYSSYSK
                     SIELGFTEPYLFDRNIALGGDIYRRDYRSFNLLGGERNTTYEQVTTGGQIRSGLPLTE
                     NMSLALRYSLNFDEVSLDESQFFSDLDGDGVATCDPILAGRYLCQAIGDRTTSAAGYS
                     FVYDTLDNRLRPTAGHRMILSQDFAGLGGTTKYLRTRAEASKYWGIGDSGFVLSGRVE
                     GGYIHSFEKSDDEGEDPVRLTDRFFLGEPQIRGFDIRGVGPRIQRIPYLFERDEDGNA
                     VLNTDPDQITDDALGGRAYYLARAELEIPLGAKGRELGLRPSVFVDVGALFGLKNPQT
                     LDLEPDDPALTRPILSSEGNFQCVEAQGNDVIQIPVGGTCPTGTQLFANRLAPFKEIY
                     VGDTPKPRISVGFGVNWNSPFGPFRVDIAKALTKAEGDDTKLFTFNVGTAF"
     gene            3051..3644
                     /locus_tag="VF589_09495"
     CDS             3051..3644
                     /locus_tag="VF589_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX8257858.1"
                     /translation="MTKFALGAALAALSIAIPAAAPAQRIGAAVVAVVDTGRISRECT
                     ACRAATAQLQAQQNTLRTRAQTLQQQIRAEGQPIATAVNALGTRQPDAALQQRITALQ
                     TRERSAQQELSTAQVNLQSTEAHVNQQILTRLLPIVGRVAQARGANLAVERGATLFAA
                     PALDITAEVMTQLNQQLPTVSVTPLPQQQQQQRPQGR"
     gene            3644..4117
                     /gene="fabZ"
                     /locus_tag="VF589_09500"
     CDS             3644..4117
                     /gene="fabZ"
                     /locus_tag="VF589_09500"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX8257859.1"
                     /translation="MTDAGRAAIGPLDIRRVMAALPHRYPMLLVDRIEEIVPDRSITA
                     IKAVSINEDFFNGHFPGRPIMPGVLIVEALAQAAGVLAVESLGLAGSGKLVYFMAIDG
                     AKFRQPVEPGVLLRLEVEFVQKRSSVCKFAGRALVEGKLAAEANFTAMIADPPAG"
     gene            complement(4122..4817)
                     /locus_tag="VF589_09505"
     CDS             complement(4122..4817)
                     /locus_tag="VF589_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8257860.1"
                     /translation="MSLVRASGLALPDRLDPTDLVLEAGTLTCLVGPNGSGKTSLLHA
                     LAGIGAPAGRVEIGGVDPASLGPAHRSRALTFLPASRDVKWPLTAIDLIRLGASGPIH
                     DSLAALELGGLADRRIDRLSTGERGRVLIARALAPRPRLLLLDEPVANLDPLWQLRLM
                     EHLRALVRREGAAALVAMHDLDLAARFGDRLIVMDRRRIAADGEPHGLLEGPAMREVF
                     GIERSDGGWRPVG"
     gene            complement(4814..5776)
                     /locus_tag="VF589_09510"
     CDS             complement(4814..5776)
                     /locus_tag="VF589_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX8257861.1"
                     /translation="MTARLLILAALAVAFFLSLAWPWQSLAGLIEQDTELALALLVEL
                     RLPRALLALVYGATLGASGAAVQALFANPLASPDLTGTTSGAALGAVVTAYLLGFAAP
                     LALSIGSVAGALGALLLLLLLAGRRAETATLLLSGLAISALAGALTTLALALAPSPFA
                     FYDAYDWLMGSLVDRSLAQASFAAVPAFVTIALLLRTRNALDSLVLGEEVAESLGHDI
                     GRLRIRIVCLTALGVGSCVAICGAIGFIGLVAPIFARRLTRGHPGRAIVPAALLGGLL
                     LLAADLVTRAAPLGRTLPIGVVTALLGAPFFLWLVAHMRWRLSQ"
     gene            complement(5773..6576)
                     /locus_tag="VF589_09515"
     CDS             complement(5773..6576)
                     /locus_tag="VF589_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257862.1"
                     /translation="MRAFAFASAASLAFTATAGAAPADAAPRRVASLNLCTDELLLML
                     AREDQILSISFLSQLRAESPLWKRARRHRRNDGSLLSVAGLRPDLVLTMGGGARDRTG
                     IAGRLGIRTLDLPFPRSLDDVEVSLRRVAAALERPAAGALLTARIRQLRRARPARQAE
                     TIWLGGGGRSLAANGLGAQWMALAGFGQRPLRGDIVPLEHLLVRPPAILLRSDYRSGQ
                     YSGEQRWLRHPLAKGIARSRTIVTDGRAWTCMGPLMIAEIARLRATMTP"
     gene            complement(6545..8452)
                     /locus_tag="VF589_09520"
     CDS             complement(6545..8452)
                     /locus_tag="VF589_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8257863.1"
                     /translation="MHILFLISAAAAPAPVPVPAAEPSDAIVVTAARLPVAGEEAPAS
                     VTVFDAAEIEALGEPFALDYVRLSPGASVSVSGAEGSQAQIRIRGAEANQTLLFVDGI
                     AFNDVASDNQARFETFAADGLERIEVIRGPQSALWGSEALGGVIALGTPDPLAGRRGS
                     GLVELGSRDSGRAAAAFALGGPQGGVAGTAAWSRSDGIDIFGGGAGDFDGYESRSLSL
                     KGVLRPLPDVELGVVGRAIRHDNDYDGTDENFLRADTLDASEAETYAGRVWAQAGLGE
                     DAPWSVKAEAQHLDNQNRNFDGAAHVNDSFGRRTRFGVQAVRRFATGAARHALIAAVE
                     REDEDFATRDRLFGGGSDVDHSRGRTALVGEWRAEWSPRVTTDMAVRRDDFNRFEDET
                     TIRAAATVEVAEGLSLFAAYGEGIAQPGFAELFGFAPNTGFVGNAELTPERSKGYEAG
                     LRWRGRSFSAELAAFSNDLTDEIVYEQLTPFPAPTYRYVNSDGKSRRRGIEASVEWRP
                     AAGLSVAANYSFLDAREPDLGAGVPPRETRRPRHSANLFATWTADRLSLAGSASYIGR
                     RRDVDFDTFEEVRLDDYVLVGARAAYALTPQLELYGRIENGLGADYQDVFGYNTPGRS
                     VYAGLRVRLGR"
     regulatory      complement(8547..8703)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8811..9041
                     /gene="rpmE"
                     /locus_tag="VF589_09525"
     CDS             8811..9041
                     /gene="rpmE"
                     /locus_tag="VF589_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX8257864.1"
                     /translation="MKTDAHPDYHMIKVQMTDGTVFETRSTWGKEGDTLQLDIDPKVH
                     PAWTGGHGKMLDTGGQVARFNKRFGGLTLGKK"
     gene            9127..9717
                     /locus_tag="VF589_09530"
     CDS             9127..9717
                     /locus_tag="VF589_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8257865.1"
                     /translation="MLKPVQHDDAAGVPVLETERLILRAPGADDLDASTAMWSNPDVV
                     RHIGGTAFTREEVWSRILRARGLWAMLGYGYWAVYERASGRFVGDVGFADFHRALQPS
                     IEGVPEMGWVLDPWCHGRGYASEAVRAALDWAEQTLPAPEYACIIDPANAPSIGVALK
                     FGFQQIAATEYKGGPTLVFRRPGLPARAAAAPAASA"
     gene            complement(9676..11427)
                     /locus_tag="VF589_09535"
     CDS             complement(9676..11427)
                     /locus_tag="VF589_09535"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX8257866.1"
                     /translation="MRLPLPLLALLGALALLLPGAARADERILHFLSDVTVERDGTLE
                     VAETIRLRAEGDEIRRGILRDFPTRYTDRLGRRVTVGFEMVRVERDGKAEPWAREGLS
                     NGVRIRIGDADVLLDPGEHVYVIRYRTTRQLGFFDTFDELYWNATGTGWTFPIDVAEA
                     VIRLPSPAAFGDRAVYTGPEGSTARYAEVVAERPGEIRFRTTAPLYAYEGLTVAAAWP
                     KGVVEPPAAGTKAGWWLRDNGPLALGILGLFGVLAYYFHAWRRAGRGPQAGTVVPLFT
                     PPDGLSPAAVRHLWRMGSDNRTFAAALIDLGVRGCLRLREEDGGFFSGRKTYIERREG
                     ADTAALPTPEAGMMSALFGGGDSLLMDDKYHATFRAARNALDHALDKAHKGKRFLTNW
                     HWSVGGVLLMAGASAVTALVLAALDGPDIEGARGLPMLIPLLALPVVLSAFWWMKAPT
                     REGRALMDRIEGFRRYLSITEEERLETLHPPEKTPELFERYLPYAIALDVENEWAERF
                     ATVLAAAAAAGQAGTMSWYSGRSDPWNDTGGFVDRVGSSLTSSVSSASTAPGSSSGSS
                     GGGSSGGGGGGGGGSGW"
     gene            complement(11427..11996)
                     /locus_tag="VF589_09540"
     CDS             complement(11427..11996)
                     /locus_tag="VF589_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8257867.1"
                     /translation="MDIGGWIVVAVVAVVLLWLIVIYNRLVRLRALVKEGWSGITVQL
                     RRRADLIPNLVSTVEGYATHERDTLNEVTAHRTDPMKARGVEATAQADTAFSAMLGRL
                     FAVAEDYPDLKADANFRQLQDELAEVEEELQGARRYYNATVRDLNTQVQSFPDVLVAK
                     PIGFSEEPFYADEDPSIQAAPKVSFGARA"
     gene            complement(12148..12543)
                     /locus_tag="VF589_09545"
     CDS             complement(12148..12543)
                     /locus_tag="VF589_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257868.1"
                     /translation="MLKPITLALAACALIAGPAQAQRTDPETRLAREIEGRVAGEPVD
                     CINLRDIRSSRIIDRTAIVYETGGGTIYVNRPDGGASSLDRWDTLVTDTPSSRLCDID
                     VVKLYDTSTRMQTGFVFLGEFVPYRKVRR"
     gene            complement(12611..13723)
                     /locus_tag="VF589_09550"
     CDS             complement(12611..13723)
                     /locus_tag="VF589_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX8257869.1"
                     /translation="MDKPLAGIKVVELARILAGPWAGQLLADLGAEVVKVERPGSGDD
                     TRGWGPPFVKGEGSAAYFHGCNRGKSSLAVDLETQAGQERVKALVAEADILIENFKVG
                     GLARFGLDYAALSALNPRLIYCSITGFGQDGPYAPRAGYDFMIQGMGGIMSLTGESGG
                     EPQKIGVAYADIFTGLYSAAAILAALNGRRATGKGCHIDMALLDTQVAVLANQAMNYL
                     VSGETPRRMGNVHPNIAPYQIFAAADGHLIVAVGNDGQFHKLCELVGLPELIADARFA
                     ANAGRVREREALTAALAPALARRPRDEWLAVFAAAGVPAGPINDVAEVFADPQVIARG
                     LRIERDGVPGVASPIVIDGKRQVAERGSPGLGGGGE"
     gene            complement(13841..15031)
                     /locus_tag="VF589_09555"
     CDS             complement(13841..15031)
                     /locus_tag="VF589_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8257870.1"
                     /translation="MAEATALPFDWADPFALDAQLSDEERLVRDTAEGYAQERLQPRV
                     TQAYLEENFEREIMSEMGELGLLGATIAPEYGGAGLNYVSYGLVARAVERVDSGYRSA
                     MSVQSSLVMHPINAYGSEEQRRKYLPKLASGEWVGCFGLTEPDAGSDPGSMLTRAQKI
                     DGGYRLSGSKMWITNSPIADVFVVWAKSDAHDGRIKGFVLEKGMKGLSAPKIGEKLSL
                     RASITGEIVMDGVEVPEENLLPNVSGLKGPFGCLNRARYGIAWGSMGAAEACYHAART
                     YTLDRKQFGRPLAATQLVQMKLANMITEITLGLQAALRVGRRMDEGELIPETISLIKR
                     NNVGKALDIARVARDMHGGNGISAEYQVMRHAANLETVNTYEGTHDEHGLILGRAITG
                     IAAF"
     gene            complement(15175..15654)
                     /locus_tag="VF589_09560"
     CDS             complement(15175..15654)
                     /locus_tag="VF589_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257871.1"
                     /translation="MDRKKRNWAIAAAAAVAIAGAGYYSARPEEAPKAKAREKTVEEQ
                     RVEELVNLMSAGSGGGGDTAQAPPAMDLPPMAAPVPMPEGEAFDMDAAARSYRRSAET
                     ADAADYYNCDGDCTGLGAGMTMDGSEAPTLGEGFGRTAPLPEARPLPEPGPLDPGPE"
     gene            complement(15971..16429)
                     /locus_tag="VF589_09565"
     CDS             complement(15971..16429)
                     /locus_tag="VF589_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257872.1"
                     /translation="MLEVKLTEGGWFEGLPASQSGVLQQLLADGSTEEQVAELWLSTP
                     GAASTAGFGAMGAIQSFYHNVKREFVAFACGDARYEDERAQALQIWKDQGKVGLVSMV
                     AAIVASTVGLAAAAVVPVIALLFSLVAKVGLNAFCASCAPAADGATAPPA"
     gene            complement(17115..17204)
                     /locus_tag="VF589_09570"
     tRNA            complement(17115..17204)
                     /locus_tag="VF589_09570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17168..17170),aa:Ser,seq:tga)
     gene            17314..18366
                     /locus_tag="VF589_09575"
     CDS             17314..18366
                     /locus_tag="VF589_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX8257873.1"
                     /translation="MRLISHAMAIALAAFPPYGAGGTAALAQSASLSQAGFQSYLPRL
                     RAEAERAGVTRSTLDRVFPTLEFSQRTVELDRAQPGGVTNPNAIPPFAPYRDRHVTSD
                     LINRGRSRYSANTGQLNAIGRRYGVTPSVLVAIWGHETSYGAVTGDFDLLNSLASLAY
                     EGRRRQLFTDEFIATLKMMDRGYSRSQLKGSWAGATGYPQFLPSVFLRLAVDGDGDGR
                     ANIWSSQADALASIANYLSNAGWKPGVPWGVAVNVPAGFNRGPVATRLMSPRCPRVHQ
                     RHSRWLSVGEWRRLGIVPQSGRLPPESELASLIEPDGPGRTAYLLTTNYRTILDYNCS
                     NFYALSVGLLADEVSR"
     gene            18459..19631
                     /locus_tag="VF589_09580"
     CDS             18459..19631
                     /locus_tag="VF589_09580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX8257874.1"
                     /translation="MRKALYFGLITTALALPATAAAPPFETPAPVAYMVDLSSGAVLY
                     AKDPDRRIPPASMAKMMTTHLAFHLIKKGDVKLQQMCDVRPETWRQWHGPKAGSTMFL
                     STGERVSVENLLHGIVTLSGNDATVVLAECIAGTEPAFVALMNAEAKRLGMTNSNFGN
                     TNGWPDEGVTVTTARDLAKLAAATILETPDLYKAFYQKESFTWGRTLGGNQPITQGNR
                     NPLLGKVAGADGLKTGHTAEAGYGFTGSAEQNGRRIIMVVAGLSSYNQRIDESVKFMN
                     WGFRAWKSQPLFTRGETVANANVQLGGEDQVALLAPRDLAVTLPAGLVSGNDMRVKVV
                     YDGPLKAPIAKGQHVANLIVQTGDTPPQSMPLVAAEAVEEAGFFGRMWNGLMSLFA"
     gene            19633..20259
                     /gene="tmk"
                     /locus_tag="VF589_09585"
     CDS             19633..20259
                     /gene="tmk"
                     /locus_tag="VF589_09585"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX8257875.1"
                     /translation="MVGGRFISLEGGEGAGKSTQLRRLADALRARGREVVETREPGGS
                     PGAEAIRALLLEGEPDRWTPEAEALLFAAARADHVARNIRPALARGAWVLSDRFLDSS
                     LAYQGGAGGVGAQAIRALHAIGSGGLLPDRTLLLELPAAAAAGRAGDRDRGAADRIGG
                     READYHAEVAAAFAALAAEEPARFRRIDASGAPEAVTERLLGAIADLS"
     gene            20256..21242
                     /locus_tag="VF589_09590"
     CDS             20256..21242
                     /locus_tag="VF589_09590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX8257876.1"
                     /translation="MTPLYGHAAAVAAFKHGLASGRLHHAWLISGPGGVGKATFAAKA
                     ALRVLAEGAGPTVDAPGLDVPDGHRMAHLVEAGSHPDLMRLERLPKESGELARSITVD
                     QVRGLARLFGTTATFSPWRVVIIDAVDDLEPGGANALLKNLEEPPANGLFLLVSHAPE
                     RLLPTIRSRCRQLRLSRLDDDAMTSALRAALPDADHAEIAALAAVGEGSPGRAIAFRG
                     LDIGALDAAMLELVAEGDPTNARRSKLASALALKPAQPRYEAFLARAPALIAAESKSR
                     RGPALVEALRLYERASELAGTAVRLSLDPQTVAFELAGMLAALADPAARRTG"
     gene            21276..22838
                     /gene="metG"
                     /locus_tag="VF589_09595"
     CDS             21276..22838
                     /gene="metG"
                     /locus_tag="VF589_09595"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX8257877.1"
                     /translation="MATPFYITTAINYPNGRPHVGHAYEAIAADAIARFQRLMGRDVI
                     FQTGTDEHGLKMAQTARDRGVEPAELADEMSSYFRDMDAKLNISCDRFIRTTEADHHR
                     ASQAIWQAMADSGDLYLGRYEGWYSVRDEAYYDEKEIVTAESGEKLSPQGTLVEWTVE
                     ESWFFRLSKYQEPLLRHYRDNPDFIRPEARRNEILRFVEGGLSDLSVSRTSFDWGVKV
                     PGSDRHVMYVWVDALTNYITGVGYPDLESAAYKRYWPADLHIIGKDIVRFHAVYWPAF
                     LMSAGIELPRQVFGHGFVLHRGEKMSKSLGNVVDPLDLAAAFGVDPLRYFLLREVTFG
                     QDGSYSAEAIVTRVNADLANSFGNLAQRTLSFIAKNCDGILPQGGKGDPADQALLDLV
                     AEVTRREMPAHFEALALSQGIEAWLRAVFACNQYIDTQAPWALRKTDPERMGAVLAAL
                     FQAIGDLAIAIQPVIPAAAAALLDWMGIPNGERDFAALGDSGRYERLAASGFRLGLPS
                     PIFPRLDMPADA"
     gene            22922..23431
                     /locus_tag="VF589_09600"
     CDS             22922..23431
                     /locus_tag="VF589_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257878.1"
                     /translation="MRTLASTFGSRAEAEAAGRRLESIGIARDRIILKDVGEAGGEPG
                     GASGAAAAGVFVSVKVTTDQVAGASEILKSSRPAGDGAGAVSSGREAASPPAPSPDVQ
                     PRVAPAAAPKPDPVPRAASQAAATPPHAPGAATIAGLSRAQLIRYFALFCVALVAAFL
                     LGAGLGLIL"
     gene            complement(23428..24264)
                     /locus_tag="VF589_09605"
     CDS             complement(23428..24264)
                     /locus_tag="VF589_09605"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX8257879.1"
                     /translation="MPRTVTARFASEEEAERALAAISAEVPLRDSAVIGSGPAGALTL
                     DSLQLTPEERSACEAQVAKGGYLLVAQVASESRGAAVLRLLGGISGGGAPEGAAAPAP
                     APAPAAEAAPPPPPAPERRVEAPEAVAAPLAAAAAATVEEEERIPIVEEELRIGKREV
                     VRGRSRVHTHIAEIPVQEDVELLHEETRLERRPVNRRLTEEEVAEGGLLQERVIEITE
                     MREEAVVTKEAFVREELVVTKSVHRRVERIEETVRRTEVETERLEPETAAAGGGGRAD
                     GR"
     gene            complement(24264..>24620)
                     /locus_tag="VF589_09610"
     CDS             complement(24264..>24620)
                     /locus_tag="VF589_09610"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEX8257880.1"
                     /translation="VEAGRLRVRVSVDEREERVPVTLTHDEVVIERVPKNIPLSELPS
                     VRLEGNTTIIPVVEEVVVVEKRLMLIEEIHVRRKAASSTEEVAVTLRSEQASIERDGT
                     GGGGDVAGAGHSEEEG"
BASE COUNT         3941 a         8963 c         7966 g         3750 t
ORIGIN      
        1 ccgctgctcg acggcgggca tctcctcttc tacacgatcg agggtgtgcg ccggaaaccg
       61 ctcaggccgg aggcgcagga atgggcgttc aggaccgggc tggcggcgct gctcgctctg
      121 atgatctttg ttaccctcaa cgacctggcg tcttttggag tgtggacgag gcttggcggg
      181 ttgatcgggt gagccgcttc gggcagggcg gtaacacagg gtaataaagg ccggggaaat
      241 aacctggccg caaggctcaa agggcgggga cgcgtgacag cgatcaaagc attttcaagt
      301 agtaagcggc tgtgctcggc ccttttgatg ggtacgatgc tgggcggctt cgccgtgccc
      361 gctcacgccc aggacgcgca ggccgcgccc gaggcttcgg ccccggcgca ggatcctgcc
      421 gcgccggtgg caccgcccgc cgcaacgccg gcggacgcgc aggcgccggt cgccggcggc
      481 ggcgtgcccg cggccgccgc gccgatcgtg gccccggcgc cgaccggcac ggtccgctcg
      541 atcaccgtca ccggcaacca gcggctggag acggagacgg tcgtctccta catgccgctc
      601 cgggtcggcg agccctatac gcgcgaggcg ctcgatgagg cgctgcgcga cctttatgcg
      661 accgaattgt tcgccgacgt tcagatccgc gacaacctcg gcgcgctgac ggtggaggtc
      721 cgggagaatc cggtcatcaa ccgcatcatc ctcgaaggca acaagcgcat caaggacgcc
      781 aagatcgcgc ccgaaatccg gctggctccg cgccagatct tcacccggtc caaggtccgc
      841 gccgacgtcg cccgcatcat cgagctgtat cgccgccagg gccgcttcgc cgcgaacgtc
      901 gagcccaaga tcgttcagct cgagcagaat cgcgtcgacc tcgtcttcga gatcaacgaa
      961 gggccgcgct ccaaggtgcg cagcatcaac atcctcggca acgcgcgctt ttccgacggc
     1021 cggttgcggt ccgagatgga gacccgccag accagcctgt tgagcttcct cggttcggcc
     1081 gacacctacg atcccgaccg gctcgcctac gaccagcaga agctgcgtca attctacctg
     1141 accgaaggct atgccgactt ccgcgtcgtc tcggcggtgg ccgagctcac ccccgaccgg
     1201 cgcgacttca tcatcaccta cgtcgtcgag gaaggcgatc gctacaagtt cggcgacgtc
     1261 aaggtcacca gcggcattcg cgacttcaat ccggagacgc tggccaaggg cctgccgatg
     1321 aagaccggcg actggtacga cgccaaagag attgaagaca ccgtcacctc gctgaccgag
     1381 acggccggcg tgttcggcta cgccttcgcg caggtccagc cgcaattcga gcgcaacaaa
     1441 gaaactctca ccatgtcggt gaacttccag attgcggaag cgccgcgcgt ctatgtcgag
     1501 cgcatcgaca tcaacggcaa cacggtgacg cgcgacaagg tcatccgccg cgagttcagg
     1561 ctggcggaag gcgatccgtt caacagcatc caggtgaagc gttcccgcga ccgcatccag
     1621 tcgctgggct tcttccagga caatctcgag atcgagcagc agcccggctc cactccggac
     1681 cgcgtcgtgc tggccgtcga cgtcgaagag aaatcgaccg gcgacatcca gctgtcggcg
     1741 ggcttctcga gcctcgaacg cttcctggtc aacgcctcga tccgccagcg caacttcatg
     1801 ggcaagggcc aggaagttcg cgccgcgatc aactattcga gctattcaaa gtcgatcgaa
     1861 ctcggcttta ccgagcccta tctgttcgac cgcaacatcg cgctcggcgg cgacatctac
     1921 cggcgcgact atcgcagctt caacctgctc ggcggcgagc gcaacacgac ttatgagcag
     1981 gtgacgaccg gcggccagat ccgctcgggc ctgccgctca ccgagaatat gtcgctggcg
     2041 cttcgctaca gcctcaattt cgacgaggtg tcgctggacg aaagccagtt cttctccgat
     2101 ctcgacggcg atggcgtggc gacctgcgac cccatcctgg ccggccgcta tctgtgccag
     2161 gcgatcggcg accgcacgac ctcggcggct ggctattcct tcgtctatga cacgctcgac
     2221 aaccggcttc gcccgaccgc cggccatcgc atgatcctga gccaggattt cgcgggcctc
     2281 ggcggcacca cgaaatatct gcgcacccgc gccgaagcga gcaaatattg gggcatcggc
     2341 gacagcggct tcgtgctgtc gggccgcgtc gaaggcggct acatccacag cttcgagaaa
     2401 agcgacgacg agggggagga tccggtccgc ctcaccgatc gcttcttcct cggcgaaccg
     2461 cagatccgcg gcttcgacat tcgcggcgtc ggcccgcgca tccagcgcat cccctatctg
     2521 ttcgagcgcg acgaggacgg caacgccgtg ctcaacaccg accccgacca gatcaccgac
     2581 gacgcgctcg gcgggcgggc ttattacctc gcccgcgctg agctcgaaat cccgctcggc
     2641 gccaagggcc gcgagctcgg gctgcggccg tcggtcttcg tcgacgtcgg cgccctgttc
     2701 gggctgaaga atccgcagac gctcgatctc gaacccgacg accccgcgtt gacgcggccg
     2761 atcctgtcgt ccgagggcaa cttccagtgc gtcgaggcgc aggggaacga cgtcatccag
     2821 attccggtcg gcggcacctg cccgacgggc acccagctgt tcgccaaccg gctcgccccg
     2881 ttcaaggaaa tctatgttgg cgacacgccc aagccgcgca tctcggtggg ctttggggtc
     2941 aactggaact cgcccttcgg gccgttcagg gtcgatatag ccaaggcgct taccaaggcc
     3001 gaaggggacg acaccaaact cttcacattc aacgtaggaa ccgcattctg atgaccaaat
     3061 tcgctctcgg cgcggccctt gccgcgctct cgatcgccat ccccgccgcc gctccggcgc
     3121 agcggatcgg cgctgccgtg gtagccgtgg tcgacacggg tcgcatctcc cgcgaatgca
     3181 cggcctgccg cgcggcgacc gcgcagctcc aggcccagca gaacacgctg cgcacccgcg
     3241 cccagacgct ccagcaacag atccgcgccg aaggccagcc gatcgcgacc gccgtcaatg
     3301 cgctcggcac ccgccagccg gacgcggcgc tgcagcagcg gatcaccgcg cttcagaccc
     3361 gcgagcgcag tgcccagcag gagctgtcga cggcccaggt caacctgcaa tcgaccgagg
     3421 cccacgtcaa ccagcagatc ctcacccggc tgctgccgat cgtcggccgg gtcgcgcagg
     3481 cgcgcggggc caatttggcg gtcgaacgcg gcgcgacttt gttcgccgcc cccgccctcg
     3541 acatcaccgc cgaagtgatg acgcagctca atcagcaact cccgaccgtc agcgtcacgc
     3601 cgctgccgca gcaacagcag cagcagcgcc cgcagggccg ctgatgacgg acgccggcag
     3661 ggccgccatc ggcccgcttg acatcaggcg ggtgatggcg gcgctgccgc accgttatcc
     3721 gatgctcctc gtcgaccgga tcgaggagat cgtccccgac cgatcgatca ccgcgatcaa
     3781 ggcggtgtcg atcaacgagg atttcttcaa cggccacttc ccgggccgcc cgatcatgcc
     3841 gggcgtgctg atcgtcgagg cgctggccca ggcggcgggc gtgctcgcgg tcgaatcgct
     3901 cggcctcgcc ggctccggca agctcgtcta tttcatggcg atcgacggcg cgaaattccg
     3961 ccagccggtg gagcccggcg tgctgctccg cctcgaagtc gagttcgtcc agaagcgcag
     4021 cagcgtctgc aagttcgccg gccgtgcgct ggtcgagggc aagctcgcgg ccgaagccaa
     4081 tttcaccgcc atgatcgccg acccgccagc gggctagaca tctagccgac cgggcgccag
     4141 ccgccgtcgg accgctcgat cccgaacacc tcccgcatcg ccggcccttc gagcagcccg
     4201 tgcggctcgc catccgcggc gatgcggcgg cggtccatca cgatcagccg gtcgccgaac
     4261 cgcgcggcaa gatcgaggtc gtgcatcgcg accagggccg ccgcgccctc gcgccggacc
     4321 agcgcccgca aatgctccat cagccgcaac tgccagagcg ggtccaggtt cgccaccggc
     4381 tcgtcgagca gcagcagccg cggccgcggc gccagcgcgc gcgcgatcag cacgcggccg
     4441 cgctcgccgg tcgagagccg gtcgatcctc cggtcggcga ggccgcccag ctcgagcgcc
     4501 gccagactat cgtggatcgg cccgctcgcg cccagccgga tgaggtcgat cgcggtgagc
     4561 ggccatttga cgtcgcgcga ggcgggaagg aaggtcagcg cccgcgagcg gtgtgcgggg
     4621 ccgaggctcg ccggatccac gccgccgatc tcgacccggc cggcgggcgc gccgatgccg
     4681 gcgagggcgt ggagcaggct ggtcttgccg ctgccattgg ggccgaccag acaggtgagg
     4741 gtgcccgcct cgagcacgag gtcggtaggg tcgaggcggt ccggcagcgc cagcccggag
     4801 gcgcggacga ggctcattgg gagagcctcc agcgcatatg cgccaccagc cacaggaaga
     4861 agggcgcgcc gagcaaggcg gtgacgacgc cgatcggcag cgtccgtccg agcggcgccg
     4921 cccgtgtgac gaggtcggcg gccagcagca gcagcccgcc gagcaacgcc gccggcacga
     4981 tcgcccggcc cggatggccc cgcgtcagcc gccgcgcgaa aatcggggcg accaggccga
     5041 tgaagccgat cgcgccgcag atcgcgacgc acgagccaac ccccaaggcg gtcaggcaga
     5101 cgatgcggat gcgcagccgc ccgatatcgt gccccagcga ttccgccacc tcctcgccga
     5161 gcacgaggct gtcgagcgca ttgcgggtgc ggagcagcag cgcgatggtg acgaaagcgg
     5221 ggaccgcggc gaagctcgcc tgggcgaggc tgcggtcgac cagcgaaccc atcagccaat
     5281 cataggcgtc gtaaaaggcg aagggggagg gggcgagcgc cagcgcgagc gtggtgaggg
     5341 cgcccgccag cgcgctgatc gccagccccg acagcaacaa ggtggcggtc tcggcccgcc
     5401 gcccggccag caggaggagc aggagcaagg cgcccagcgc cccggcgacc gagccgatcg
     5461 acaaagcgag cggggccgcg aagccgagca gataggccgt caccaccgcc cccagcgccg
     5521 ctccgctggt ggtgccggtg aggtcgggcg aggccagcgg gttggcgaac agggcctgga
     5581 ccgcggcccc ggaggctccc aatgtggcgc catagaccaa agcgagcagc gcccgcggca
     5641 gccgaagctc gaccagcagg gccagcgcga gctcggtatc ctgctcgatc agccccgcca
     5701 gcgactgcca cggccaggcg agcgacagga aaaaggcgac ggcgagcgcg gccagaatca
     5761 gcagccgcgc cgtcatggcg tcatcgtcgc gcgaagccgc gcgatctcgg cgatcatcag
     5821 cgggcccatg caggtccagg cgcggccgtc ggtgacgatc gtccgcgacc gcgcaatacc
     5881 cttagcgagc gggtgcctca gccagcgctg ctcgcccgaa tattggccgc tgcgatagtc
     5941 gctgcgcagc aggatggcgg gcgggcggac gagcaaatgc tcgagcggga cgatgtcgcc
     6001 gcggagcggc cgctggccga agccggcgag cgccatccat tgggcgccga gtccgttcgc
     6061 ggccaggctc cgcccgccgc cgccgagcca gatcgtctcc gcctggcgcg cgggccgggc
     6121 gcgccgcaat tgccggatgc gggcggtcag cagcgcgccc gccgccggac gctccagcgc
     6181 cgccgccacc cgccgcagcg atacctcgac gtcatcaagg ctgcgcggga agggcaggtc
     6241 gagcgtgcgg atgccgagcc tcccggcgat gccggtgcgg tcgcgcgcgc cgccgcccat
     6301 ggtcagcacc agatcggggc ggagcccggc gacggagagc aggctgccgt cgttgcgccg
     6361 atgccggcgc gcccgcttcc acagcggcga ttccgcccgc agctgcgaca ggaagctgat
     6421 cgaaaggatc tggtcctcgc gtgccagcat cagcagcagc tcgtccgtgc acaggttgag
     6481 cgacgccacc cggcgcgggg cggcatcagc cggcgcggcg ccggccgttg cggtgaacgc
     6541 gaggctagcg gccgaggcga acgcgaaggc ccgcatagac cgaccgcccc ggcgtattgt
     6601 agccgaagac gtcctgataa tcggcgccga gcccattctc gatccggccg taaagctcca
     6661 attgcggcgt cagcgcataa gcggcccgcg cgccgaccag cacataatcg tcgagccgga
     6721 cctcctcgaa agtgtcgaag tcgacgtcgc ggcgccgccc gatataggaa gccgagccgg
     6781 cgaggctgag ccggtccgcc gtccaggtgg cgaagagatt ggcgctgtgc cttggccgcc
     6841 gcgtctcgcg gggcggcacg cccgcgccca gatcgggctc gcgcgcgtcg aggaagctgt
     6901 aattggcggc gacgctgagg cccgccgccg gccgccactc gaccgacgcc tcgatgcccc
     6961 ggcggcggct ttttccgtcc gaattgacgt agcggtaggt gggcgcgggg aagggggtca
     7021 gttgttcgta gacgatctcg tcggtgaggt cgttcgagaa agcggcgagc tcggccgaga
     7081 agctccgccc gcgccacctg agacccgcct cgtaaccttt cgaccgctcc ggcgtaagct
     7141 ccgcattgcc gacgaagccg gtgttcggag cgaagccgaa cagctcggcg aagccgggct
     7201 gggcgatgcc ctcgccataa gcggcgaaca gcgagagccc ttccgccacc tcgaccgtcg
     7261 cggcggcgcg gatcgtcgtc tcgtcctcga accggttgaa gtcgtcgcgg cggaccgcca
     7321 tgtccgtggt gacgcgcggg ctccactccg cccgccactc gccgaccaaa gcggtgcgcc
     7381 cgcggctatg atcgacatcg ctgccgccgc cgaacaaccg gtcgcgggtg gcgaaatcct
     7441 catcctcccg ctccaccgcc gcgatcaaag cgtggcgggc cgcgcccgtc gcgaaccgcc
     7501 gcaccgcctg cacgccgaag cgggtgcgcc ggccgaagct gtcgttgacg tgggccgcgc
     7561 cgtcgaaatt tcggttctga ttgtcgagat gctgagcttc ggccttgacg ctccagggcg
     7621 cgtcctcgcc aagcccggcc tgcgcccaga cgcggccggc ataggtctcc gcctcggagg
     7681 cgtcgagcgt gtcggcgcgc aggaaattct cgtcggtgcc gtcatagtcg ttgtcgtggc
     7741 ggatcgcgcg cccgaccacg cccagctcga cgtcggggag cgggcgcagc acgcctttga
     7801 ggctgaggct gcgactctca tagccgtcga aatccccggc gccgccgccg aatatgtcga
     7861 tgccgtcgct gcgcgaccag gcggcggtcc cggcgacgcc gccttgcggg ccgccgagcg
     7921 cgaaggccgc cgccgcgcgc cccgaatcgc ggctgccgag ttcgaccagg ccgctgccgc
     7981 gccgcccggc gagcggatcg ggcgtcccca gcgcgatcac gcctcccagc gcttccgatc
     8041 cccacaaggc cgattgcggc ccgcggatca cctcgatccg ctcgagcccg tcggcggcga
     8101 aagtctcgaa ccgcgcctga ttgtcggacg cgacgtcgtt gaaggcgatg ccgtcgacga
     8161 acagcaaggt ttggttggcc tcggcgccgc ggatccggat ctgcgcctgg gagccctcgg
     8221 cgcccgacac cgacaccgaa gcgccgggcg acagccgcac ataatcgagc gcgaacggct
     8281 cgcccagcgc ttcgatttcg gcggcgtcga agaccgtgac cgaagccggc gcctcctcgc
     8341 ccgcgaccgg caggcgcgcg gcggtgacga cgatggcatc ggacggctcg gccgccggca
     8401 cggggacggg cgccggcgcg gccgcggcgc tgatcaggaa caggatgtgc atgtcaacct
     8461 ccacgaaacg acaggattgc cgtctcggag gtatcgcggg cgcaatgcgc ccccgacgcc
     8521 acgcaccgca ggctggaccc ggccgggtgc ggacgacgag cggaggcagt tttctggctt
     8581 acggctcgcc gcctgctttg gaacaggagg cttgccgctt acagttgcgg gcacagcgcc
     8641 ggtctttcac cggcttgcct tttaaccgcc gtttccggcg gtaccttcgc tgcggccggg
     8701 gctaatcccg cgccgcacgt cttgtcaact ttcggttgag acggtagagc gcccgccaga
     8761 ccttcccagg aggctggtcg gaaccagccg aaactcagga aaaatcagcc atgaagaccg
     8821 acgcccatcc cgattaccac atgatcaagg tgcagatgac cgacggcacc gtgttcgaga
     8881 cccgctccac ctggggcaag gaaggcgaca cgctgcagct cgacatcgac cccaaggtcc
     8941 acccggcctg gaccggcggc cacggcaaga tgctcgacac gggcggccag gtcgcgcgct
     9001 tcaacaagcg ctttggcggc ctcaccctcg gcaagaaata agcccgggca ttcggtctcg
     9061 gctcgatcgt caccttgaac tcgtttcagg gtccatccgg cgggccccgc agaggccggt
     9121 agctggatgc tgaaaccagt tcagcatgac gatgcggccg gcgtcccggt cctcgagacc
     9181 gagcgcctca tcctccgcgc ccccggcgcc gacgatctcg acgcctccac ggctatgtgg
     9241 agcaatcccg acgtcgtccg ccacatcggc ggcaccgctt tcacccgcga agaggtgtgg
     9301 tcgcggatcc tgcgcgcccg cggcctctgg gcgatgctcg gctacggcta ttgggcggtc
     9361 tatgagcgcg ccagcggccg cttcgtcggc gacgtcggct ttgccgattt ccaccgcgcg
     9421 ctccaaccgt cgatcgaggg cgtgcccgaa atgggctggg tgctcgatcc ctggtgccac
     9481 ggccgcggct atgccagcga ggcggtccgc gccgccttgg actgggccga gcaaaccctc
     9541 cccgcgcccg aatatgcctg catcatcgat cccgccaacg cgccgtcgat cggggtggcg
     9601 ctgaaattcg gcttccagca gatcgccgcg accgaatata aaggcggccc gactttggtc
     9661 ttccggcgcc cgggcctacc agcccgagcc gccgccgccc ccgccgcctc cgcctgagga
     9721 gccgccgccc gacgagccgc tgctcgaacc cggcgccgtg gaggccgaag acacgctgct
     9781 ggtcagcgac gagccgaccc ggtcgacgaa gccgccggtg tcgttccagg gatcgctgcg
     9841 tcccgaatac cagctcatag tccccgcctg gcccgcggcc gccgccgccg ccagcacagt
     9901 ggcgaaccgc tccgcccatt cattctcgac gtcgagcgcg atcgcatagg gcagatagcg
     9961 ctcgaacagc tcgggcgtct tttccggcgg atgcagcgtc tccagccgct cctcctcggt
    10021 gatcgacaga tagcggcgga agccttcgat ccggtccatc agcgcgcgcc cctcgcgggt
    10081 cggcgccttc atccaccaaa aggcgctgag cacgaccggc agcgccagca gcgggatcag
    10141 catcggcaag ccccgggcgc cctcgatgtc cggtccatcg agcgccgcca gcaccagggc
    10201 cgtcaccgcg gaggcccccg ccatcaggag cacgccgccc accgaccaat gccagttggt
    10261 caggaagcgc ttgcccttgt gggccttgtc gagcgcgtga tcgagcgcgt ttcgcgcggc
    10321 ccggaaggtg gcgtgatatt tgtcgtccat cagcaggctg tcgccgccgc cgaacaaggc
    10381 cgacatcatc cccgcctcgg gcgtgggcag cgccgccgta tcggcgccct cgcgccgctc
    10441 gatataggtc ttgcggccgg agaagaagcc gccatcctcc tcgcgcagcc ggaggcagcc
    10501 gcgcacgccg agatcgatca aagcggcggc gaaggtgcgg ttgtccgatc ccatccgcca
    10561 caaatggcgg acggcggcgg gggacagccc gtcgggcggc gtgaacagcg gcaccaccgt
    10621 ccccgcttgc gggccgcggc cggcgcggcg ccaggcgtgg aaataataag ccagaacgcc
    10681 gaacagcccc aatatgccga gcgcgagtgg gccgttgtcg cgcagccacc agcccgcctt
    10741 ggtgccggcc gccggcggct ccaccacgcc cttgggccac gccgccgcca cggtcaggcc
    10801 ttcataggcg tagagcggcg cagtcgtgcg gaagcgtatc tcgccgggcc gctccgccac
    10861 cacctcggcg tagcgggcgg tcgacccttc cgggccggta tagaccgcgc ggtcgccgaa
    10921 cgccgccggg gagggcaggc ggatgaccgc ttcggcgacg tcgatcggga aggtccagcc
    10981 ggtgccggtc gcgttccagt aaagctcgtc gaaggtgtcg aagaagccga gctgccgcgt
    11041 ggtgcggtag cggatcacat agacatgctc gcccgggtcg agcagcacgt cggcatcgcc
    11101 gatcctgatc cgaacgccgt tgctcaggcc ctcgcgcgcc cacggctcgg ccttgccgtc
    11161 gcgctcgacc ctgaccattt cgaacccgac cgtcacccgc cgcccgagcc ggtcggtgta
    11221 gcgcgtcgga aagtcgcgca ggatgccgcg cctgatctcg tcgccctcgg cgcggaggcg
    11281 gatcgtctcc gctacctcca acgtgccgtc gcgctcgacc gtcacgtcgc tcaggaaatg
    11341 gaggatgcgc tcgtcggccc gcgccgcccc tggaagcagc agcgccagcg cgccgagcag
    11401 cgccagcagc ggaagcggca gccgcatcag gcgcgggcgc cgaagctaac tttcggcgcg
    11461 gcctggatcg acggatcctc gtcggcgtag aacggctctt cggaaaagcc tatcggcttg
    11521 gcgaccagca cgtcggggaa cgattgcacc tgcgtgttga ggtcgcgcac cgtcgcattg
    11581 taatagcggc gcgcgccctg cagctcttcc tcgacctcgg cgagctcatc ctggagctgg
    11641 cggaagttgg cgtcggcctt aaggtcggga taatcctcgg cgaccgcgaa cagccggccc
    11701 agcatcgcgc tgaaggcggt gtcggcctgc gccgtcgcct cgacacccct ggccttcatc
    11761 gggtcggtgc ggtgggcggt gacttcgttg agcgtgtcac gctcgtgcgt ggcatagcct
    11821 tcgacggtgc tgaccagatt ggggatgaga tcggcgcggc gcctcagctg gacggtgatc
    11881 ccgctccagc cctccttcac cagcgcccgc agccgcacca gccgattgta gatgacgatc
    11941 agccacagca acacgaccgc gaccaccgcg acgacgatcc aacccccaat gtccatcccg
    12001 gcctccccgc tgagccaggc aaacatagat tggcttggcc gctccggcta gcccgcggcg
    12061 ccgattgcgc cgtccgcacc gcgaaagctc ggatgagccc caccgtcacc ccagcgaaag
    12121 ctgggatctc acttccttct cagcgggtca gcggcgaacc ttccggtagg gcacgaactc
    12181 gccgaggaac acgaagccgg tctgcatgcg cgtcgaggtg tcgtaaagct tgaccacgtc
    12241 gatgtcgcac agccgcgacg aaggcgtgtc ggtcaccaaa gtgtcccacc ggtcgagcga
    12301 actggcgccc ccatcggggc ggttgacgta gatcgtgccg ccgcccgtct cgtagacgat
    12361 ggcggtgcgg tcgatgatcc ggctcgagcg gatgtcgcgc aggttgatgc agtcgaccgg
    12421 ttcgccggcg acgcggcctt cgatctcgcg ggcgagtcgg gtctcggggt cggtgcgctg
    12481 cgcctgcgcc ggaccggcga tcagcgcgca cgcggcgagg gcaagagtga ttggcttcag
    12541 catgattcgg ctccttgctc cccgaatatc gaaggccggc ttaacggccg ctgacatcgc
    12601 agccacgggg ctattcgccg ccgccgccga gccccggact cccgcgctcc gccacctgcc
    12661 gcttgccgtc gatgacgatc ggcgaggcca cgcccggcac gccgtcccgc tcgatccgca
    12721 gcccgcgcgc gatcacctgc ggatcggcga acacctcggc gacgtcgttg atcggccccg
    12781 ccgggacgcc cgcggcggcg aagacggcga gccattcgtc gcgcggccgg cgggcgaggg
    12841 caggggcgag cgccgcggtc agcgcctcgc gctcccgcac ccgcccggca ttggcggcga
    12901 agcgcgcgtc cgcgatcagc tccggcaggc ccacgagctc gcacaactta tggaattgcc
    12961 cgtcattgcc gaccgcgacg atcagatgcc cgtcggcggc ggcgaaaatc tggtaggggg
    13021 cgatgttggg atggacgttg cccatccgcc gcggcgtctc gcccgagacg agatagttca
    13081 tcgcctggtt ggcgagcacc gcgacctggg tgtcgagcaa ggccatgtcg atgtggcagc
    13141 ccttgccggt cgcgcgccgg ccgttgagcg ccgccaggat cgccgccgcc gaatagaggc
    13201 cggtgaagat gtcggcatag gcgacgccga tcttctgcgg ctcgccgccg gactcaccgg
    13261 tcaggctcat gatcccgccc atgccctgga tcatgaagtc gtagcccgcc ctcggcgcat
    13321 agggcccgtc ctggccgaag ccggtgatcg agcaatagat gagccgcggg ttcagcgccg
    13381 acagcgccgc atagtcgagt ccgaagcggg ccagcccgcc gaccttgaaa ttctcgatca
    13441 ggatatcggc ttccgccacc agcgccttca cccgctcttg acccgcttgg gtctcgaggt
    13501 cgacggccag cgagctcttg ccgcgattgc agccatggaa ataagcggcg ctgccctcgc
    13561 ccttgacgaa aggcggcccc caaccgcgcg tgtcgtcgcc cgagcccggc cgctcgacct
    13621 tgaccacctc cgcgccgagg tcggcgagca gctgcccagc ccacggcccc gcgaggatgc
    13681 gggcaagctc tacgactttg atgccggcga gcggcttatc catatcgcgt caccccgggc
    13741 ctatctcacg tcacccccgc gaaagcgggg gtcccgcttc tcttccttcc accgcgcccc
    13801 aaaagaagcg ggattcccgc tttcgcggga atgacgaggc tcaaaaagcc gcaatccccg
    13861 tgatcgcccg gccgaggatc agcccgtgct cgtcgtgcgt gccctcatag gtgttgaccg
    13921 tctcgaggtt ggcggcgtgc cgcatcacct gatactcggc ggaaatgccg ttgccgccat
    13981 gcatgtcgcg ggcgaccctg gcgatgtcga gcgccttgcc gacattattg cgcttgatga
    14041 ggctgatcgt ctccgggatg agctcgcctt cgtccatgcg ccgcccgacc cggagcgcgg
    14101 cctgcagccc gagcgtgatc tcggtgatca tgttggcgag cttcatctgg acgagctggg
    14161 tcgcggccag cggccggccg aactgcttgc ggtccagcgt ataggtccgg gcggcatggt
    14221 agcaggcctc ggccgcgccc atcgagcccc aggcgatgcc gtagcgggcg cggttgaggc
    14281 agccgaacgg ccccttgagg ccgctgacgt tgggaagcag attctcctcc ggcacttcga
    14341 cgccgtccat cacaatctcg cccgtgatcg aggcgcgcag gctcagtttc tcgccgatct
    14401 tgggcgcgct gaggcccttc atccctttct cgagcacgaa gcccttgatc ctgccgtcat
    14461 gcgcgtcgga cttggcccac accacgaaga cgtcggcgat cggcgaattg gtgatccaca
    14521 tcttggagcc cgacagccga tagccgccgt cgatcttctg cgcgcgggtc agcatcgatc
    14581 cggggtcgga gccggcgtcg ggctcggtga ggccgaagca gccgacccat tcgccgctcg
    14641 ccagcttcgg cagatatttg cgcctctgct cctcgctgcc ataagcgttg atcgggtgca
    14701 tcacgagcga ggattgcacc gacatggccg agcgatagcc gctgtcgacc cgctccaccg
    14761 cgcgcgcgac caggccgtag ctgacataat tgagccccgc cccgccatat tcgggggcga
    14821 tggtggcgcc gagcaggccg agctcgccca tctcgctcat gatctcgcgc tcgaaattct
    14881 cctcgagata ggcctgcgtc acccgcggct gcagccgttc ctgggcgtag ccctccgcgg
    14941 tgtcgcggac cagcctctcc tcgtcggaaa gctgcgcgtc gagcgcgaac ggatcggccc
    15001 aatcgaacgg gagggcggtc gcttcggcca tcatatcctc atttcaggca aggccggaac
    15061 gcggcggcgg ccgcccggca gggactgcgc ccgcagctac cgccgcccgc cgcgatgatc
    15121 aaggatgttg cggccccggc tgggacctcg tcgtgtcggg cagggtcagg tcggctattc
    15181 cggcccgggg tcgagcggcc ccggctccgg cagcggcctt gcttcgggga gcggtgcggt
    15241 cctgccaaag ccttcgccga gcgtcggcgc ctcgctcccg tccatcgtca taccggcgcc
    15301 gaggccggtg caatcgccgt cgcaattata ataatcggcg gcgtccgcag tctcggccga
    15361 ccggcggtag ctcctggcgg ccgcatccat gtcgaacgcc tcgccctccg gcatcggcac
    15421 cggggcggcc atcggcggca gatccatggc agggggcgcc tgcgccgtat cgccgccgcc
    15481 gccggagccc gccgacatca gattgaccag ctcttcgacc cgctgctctt cgaccgtctt
    15541 ctcgcgggcc ttggccttcg gagcttcctc gggacgcgcc gaataatagc ccgcgccggc
    15601 gatcgctacc gccgccgccg ccgcaatcgc ccaattccgc ttcttcctgt ccacttgccc
    15661 cggctctccg aaaaacaccg cccgattagc agagaacccg cccgccgcca ccgggaaacg
    15721 gcggctaggg gcggtcgggc cgtgcccggg gaagaaaggg tgctggcgga tgctgtggcg
    15781 ctacgcctcc gggtagaggt actgaggcgg gccatagact cgagcaccct cgattactgg
    15841 cggaacacga ttcgagccgc cgcgggagga tggcaggcgg atcgcggttg agaggtttgg
    15901 tagtcaggcg gaaaactgaa cctgtgcccg cgcgcttcca gcgcgacgtt cgtcattgct
    15961 caatgggtgg tcaggcgggt ggggctgtgg ctccatctgc agccggggcg cacgacgcgc
    16021 aaaaagcatt gaggccgacc ttggcaacta gagaaaacag gagagcgatg acaggcacca
    16081 cagcagctgc cgctaggcct accgtgcttg ccacgatagc tgcaaccatg ctcaccagac
    16141 ccacttttcc ctgatccttc cagatttgaa gcgcctgtgc ccgttcatcc tcatatcggg
    16201 catcgccaca cgcgaatgcg acaaactccc gcttcacgtt gtggtaaaag ctctggatag
    16261 ctcccatcgc accaaatccg gccgtggatg ctgctccggg agtggagagc catagttcgg
    16321 cgacttgctc ttcggtgctg ccgtcagcca aaagctgctg aagcactccg gattggctgg
    16381 ctgggagacc ttcgaaccaa ccgccctctg tgagtttaac ctccaacatc acaggagccc
    16441 cttaatatat tcagctgaaa cagcgtgtgt agtctggtga agagtttggg gatcgatccc
    16501 tccgatgata ataccacctg ctgcacccgt gggagagtat cctcctgtta tcatagcaca
    16561 aactgtcctg ccgtcagctg tgaacacggg tccgccagac tgtcccggcc gagtttgagt
    16621 gttcaacacg acatgtttca ctttaacccc gcccgccccc aatagaacgc gagcacccac
    16681 catgctcgac tgttgcgtca ggacaagcct gccatgatcc gcatgagggt agcctagccc
    16741 gaggacgggt gtgccgggga atatctcgtc cgtgcttcca aggacgtaac caaacgagat
    16801 tgagggaggt tgggacgacc atttctaaaa tcgctacatc atggatcggg tcataggtcg
    16861 cgactttcag cgggatggcc tgcacttgct ggtcggtggt gtcttgataa tcgttgagcg
    16921 aattggttcg acctatcact gctaccaagc cctggtcgct cgggcctacc acgtgggcgg
    16981 cggtagcgaa tcgcttagga tcgacagcaa agcacgtgcc gagaagcccg actgctccac
    17041 cggcgcctcg ccgagcgagc ggcagcacga cccttccgat gtgtaagtcc aatgatcctt
    17101 tcccttctat atcctggcgg atggggtggg attcgaaccc acggtgggcg tgaacccacg
    17161 gcggttttca agaccgctgc cttaaaccac tcggccaccc atccgggccg cttggcctta
    17221 agcgccaagc ccgcgcgctt gcaagcagcc ggggaaccag cccggcattt gcgcgcttgt
    17281 cggccaagac ccgcgaccat aaagggcacc tgtatgcgtc ttattagcca cgccatggcg
    17341 atcgccctcg cggcctttcc cccttatggc gccggcggca ccgccgcgct cgcgcagagc
    17401 gcctcgctga gccaggccgg cttccaatcc tatttgcccc ggctccgcgc cgaggccgag
    17461 cgcgccgggg tcacccgctc gaccctcgac cgcgtcttcc cgacgctcga gttcagccag
    17521 cgcaccgtcg agctcgaccg cgcccagccc ggcggcgtca ccaaccccaa cgccatcccg
    17581 cccttcgcgc cctatcgcga ccgccacgtc acctccgacc tcatcaaccg cggccgatcg
    17641 cgttattccg ccaacaccgg ccagctgaac gcgatcggcc ggcgctacgg cgtcacgccg
    17701 tcggtgctgg tcgcgatctg gggccatgag accagctacg gcgccgtcac cggcgatttc
    17761 gacctgctga actcgctcgc cagcctcgcc tacgaaggcc gccggcgcca gctcttcacc
    17821 gacgagttca tcgccaccct caaaatgatg gaccgcggct attcgcgctc gcagctcaag
    17881 ggcagctggg ccggcgccac cggttatccg caattcctgc cctcggtgtt cctgcgcctc
    17941 gcggtcgacg gcgacggcga cggccgcgcc aacatctggt cgagccaggc cgacgcgctc
    18001 gcctcgatcg ccaattatct ctccaacgcc ggctggaagc cgggcgtgcc gtggggggtg
    18061 gcggtcaacg tccccgccgg cttcaaccgc ggcccggtcg ccacccggct catgtcaccg
    18121 cgctgcccgc gcgtgcacca gcgccacagc cgctggctca gcgtcggcga atggcgccgg
    18181 ctcggcatcg tgccgcagag tgggcggctg ccgccggaga gcgagctggc cagcctgatc
    18241 gagcccgacg gccccggccg caccgcctat ctcctgacca ccaactaccg caccatcctc
    18301 gactataatt gctcgaattt ctacgcgctg tcggtcggcc tgctcgccga cgaggtttcg
    18361 cgctgatcgc gctggcgcag cccgaatcga cccgttaagg atagggccga cgccagtggc
    18421 gtgcgggctc aacttcttca gggtagattt caaccatgat gcgtaaggcg ctgtatttcg
    18481 gtctcatcac taccgctctc gcccttcccg ccaccgccgc cgcgccgccg ttcgagacgc
    18541 cggcgccggt cgcctatatg gtcgatctct cgtccggcgc ggtgctttac gccaaggatc
    18601 cggatcgccg catcccgccc gcctcgatgg ccaagatgat gaccacccat ctcgccttcc
    18661 acctgatcaa gaaaggcgac gtcaagctcc agcaaatgtg cgacgtgcgc cccgaaacct
    18721 ggcgccagtg gcatgggccg aaggcgggct cgaccatgtt cctctcgacc ggcgagcggg
    18781 tcagcgttga gaatctgctg cacggcatcg tcaccctctc gggcaacgac gccaccgtcg
    18841 tcctcgccga atgcatcgcc ggtaccgagc cggccttcgt cgccctgatg aacgccgagg
    18901 ccaagcgcct cggcatgacc aattccaatt tcggcaacac caatggctgg cccgacgagg
    18961 gcgtcaccgt gacgacggcg cgcgacctcg ccaagctcgc cgccgccacc atcctggaga
    19021 cgcccgacct ctacaaggcc ttctaccaga aggagagctt cacctggggc cgcacgctgg
    19081 gcggcaacca gccgatcacc cagggcaatc gcaatccttt gcttggcaag gtcgccggcg
    19141 ccgacggcct caagaccggc cataccgccg aggcaggcta cggcttcacc ggctcggccg
    19201 agcagaatgg ccgccgcatc atcatggtcg tcgccggcct ctccagctac aaccagcgca
    19261 tcgacgagag cgtcaagttc atgaactggg gcttccgcgc ctggaagtcg cagccgctgt
    19321 tcacgcgcgg cgagaccgtc gccaacgcca acgtccagct cggcggcgag gaccaggtcg
    19381 ccctgctcgc gccccgcgac ctcgccgtca cgctccctgc cggcctcgtc agcggcaacg
    19441 atatgcgcgt caaggtggtc tatgacggcc cgttgaaggc gccgatcgcc aagggccagc
    19501 atgtcgcgaa cctgatcgtc cagaccggcg acacgccgcc ccagtcgatg cctttggtcg
    19561 ccgccgaggc ggtcgaggaa gcgggcttct tcggccgcat gtggaacggc ctgatgtcgc
    19621 tcttcgcctg atttggtggg cggccgcttc atcagcctgg agggcgggga gggggccggc
    19681 aagtcgaccc agctgcgccg cctcgccgac gcgcttcgcg cccgcggccg cgaggtggtc
    19741 gagacccgcg agcccggcgg cagccccggc gccgaggcga tccgcgcttt gctgctcgaa
    19801 ggcgagcccg accgctggac gccggaggcc gaagctttgc tgttcgccgc cgcccgcgcc
    19861 gatcatgtcg cccgcaatat ccgcccggcg cttgcccgcg gcgcttgggt cctctccgac
    19921 cgcttcctcg attcctcgct cgcttatcag ggcggggcag gcggcgtcgg cgcccaagcc
    19981 atccgcgccc tccacgccat cggcagcggc ggcctgctgc cggaccggac tttgctgctc
    20041 gagcttcccg ccgccgccgc cgccggccgc gccggcgacc gcgaccgcgg cgccgcggac
    20101 cgcatcggcg ggcgcgaagc ggactatcac gccgaggtcg ccgccgcctt cgccgcgctt
    20161 gccgcggaag agcccgcccg cttccgccgc atcgacgcga gcggcgcccc ggaggccgtg
    20221 accgagcgcc tgctcggcgc tatcgcggac ctctcatgac ccccctgtac ggccacgccg
    20281 ccgccgtcgc cgccttcaag cacggcctgg cgagcggccg gctccaccat gcctggctga
    20341 tcagcggccc gggaggggtc ggcaaggcga cgttcgccgc caaggcggcg ctgcgcgtgc
    20401 tcgccgaagg ggccggaccc acggtcgacg cacccggtct cgacgttccc gacggccacc
    20461 gcatggccca cctcgtcgag gccggcagcc acccggacct gatgcggctc gagcggctgc
    20521 cgaaggagag cggcgaactc gcccgcagca tcactgtcga ccaggtgcgc ggcctcgccc
    20581 gcctgttcgg caccaccgcc actttctcgc cttggcgcgt cgtcatcatc gacgcggtcg
    20641 acgatctcga gccgggcggc gccaacgcgc tcctcaagaa tctcgaggag ccccccgcca
    20701 acggcctgtt cctgctggtc agccacgccc ccgagcggct gctgccgacg atccgctcgc
    20761 gctgccgcca gctccgcctc tcccggctcg acgatgacgc catgacgtcg gcgctccggg
    20821 ccgcgcttcc cgacgccgat cacgccgaga tcgccgccct cgccgcggtg ggggagggct
    20881 ctcccggccg cgcgatcgcc tttcgcggcc tcgacatcgg cgcgctcgac gcggcgatgc
    20941 tcgaactggt cgccgaaggc gacccgacca acgcgcgccg ctccaagctc gcctcggcgc
    21001 tggcgctgaa gcccgcccag ccgcgctacg aggccttcct cgcccgcgcg ccggctttga
    21061 tcgcggccga atccaagagc cgccgcggcc ccgcgctggt cgaagcgctc cgcctctacg
    21121 agcgcgcctc cgagctcgcc ggcaccgccg ttcgcctctc gctcgacccg cagaccgtgg
    21181 ccttcgagct cgccggcatg ctcgccgcgc tcgcggatcc ggcggcgcgg aggacaggat
    21241 agcgcgcgtc tcgaaacgtc actaaggcgc ctgccatggc gacacccttc tacatcacca
    21301 ccgcgatcaa ctatcccaac gggcggccgc atgtcggcca cgcttatgag gcgatcgccg
    21361 ccgatgccat cgcccgcttc cagcggctga tgggccgcga cgtcatcttc caaacgggga
    21421 ccgacgagca cggcctcaaa atggcccaga ccgcccgcga tcgcggcgtc gagcccgccg
    21481 agctagcgga cgaaatgtca tcctacttcc gcgacatgga cgcgaagctc aatatttcgt
    21541 gcgaccgttt catccgcacc accgaggccg atcaccatcg cgccagccag gcgatctggc
    21601 aggccatggc cgattccggc gacctctatc tcggccgcta cgaaggctgg tactcggttc
    21661 gcgacgaagc ttattatgac gagaaagaga tcgtcaccgc cgaaagcggc gagaaattgt
    21721 cgccgcaggg cacgctcgtc gaatggacgg tggaggagag ctggttcttc cgcctctcca
    21781 aatatcagga gccgttgctc cgccactatc gcgacaaccc cgacttcatc cgtcccgagg
    21841 cgcgccgcaa cgagatattg cgcttcgtcg agggcggcct ctcagacctc tccgtctcgc
    21901 gcaccagctt cgactggggc gtcaaggtgc ccggcagcga ccgccacgtc atgtacgtat
    21961 gggtcgacgc gctcaccaac tacatcaccg gcgtcggtta tcccgacctg gaatcagcgg
    22021 cttacaagcg atactggccg gccgatctcc acattatcgg caaggacatc gtccgcttcc
    22081 acgccgtcta ctggccggct ttcctgatgt cggccggcat cgagctgccc cgccaggtgt
    22141 tcggccacgg cttcgtcctc caccgcggcg agaagatgtc caagtcgctc ggcaacgtcg
    22201 tcgacccgct cgacctcgcc gccgcgttcg gggtggatcc gctgcgctat ttcctgctgc
    22261 gcgaagtcac cttcggccag gacggcagct atagcgccga agcgatcgta acccgcgtca
    22321 atgccgatct cgccaacagc ttcggcaatc ttgcccagcg cactttgtct ttcatcgcaa
    22381 agaattgcga cggcatattg ccgcagggag ggaagggcga ccccgccgac caggcattgc
    22441 tcgatttagt cgccgaggtc acgcggcgcg agatgccggc ccatttcgaa gcgctggcgc
    22501 tcagccaggg catcgaagcg tggctgcgcg ccgtcttcgc ctgcaaccaa tatatcgaca
    22561 cccaggcccc atgggcattg cgcaagaccg atcccgaacg gatgggcgcc gtcctcgccg
    22621 ccttgttcca ggcgatcggc gacctcgcca tcgcgatcca gccggtcatc ccggccgccg
    22681 ccgccgcctt gctcgactgg atggggatcc ccaacggaga gcgcgacttc gccgctttgg
    22741 gcgattccgg ccgctatgaa cggctcgccg cgtccggatt ccggctcggc cttccgagcc
    22801 cgatcttccc gcggctggat atgcccgcgg acgcttgagc tccgcccggc ggaactcgcg
    22861 gggcttcccg gacttctacg aggcaaggcc aggctcttcc cggccagcag aagagatgtc
    22921 catgcgcacc ttagcttcca cgttcggcag ccgggctgaa gccgaagcgg caggccgccg
    22981 gctcgaatcg atcggcatcg cccgcgaccg catcatcctc aaggatgtgg gcgaggcggg
    23041 gggcgaaccc ggaggcgcgt cgggagcagc cgccgccggc gtcttcgtca gcgtcaaggt
    23101 caccaccgat caggtcgccg gggctagcga aatcctgaag tcgagccgcc cggccggcga
    23161 tggggccggc gctgtttcct cgggtcggga ggcggcgtcc ccgcccgcgc cgtcgccgga
    23221 cgtgcagccc cgcgtcgcgc ctgcggccgc cccgaagccg gacccggtgc cgcgcgccgc
    23281 ctcgcaagcc gccgcaacgc ctccgcacgc gcccggtgcc gccaccatcg ccggcctcag
    23341 ccgggcgcag ctcatccgct atttcgcttt gttctgcgtg gctttggtgg cggccttcct
    23401 gctcggcgcc gggctcggcc tgatcctcta gcgcccgtcc gcgcgcccac cgccgccggc
    23461 cgccgccgtc tccggctcga gccgctcggt ctcgacctcg gtccgccgca ccgtctcctc
    23521 gatccgctcg acccgccggt ggacgctctt ggtcaccacc aattcctcgc gcacgaacgc
    23581 ctccttggtg acgaccgctt cctcgcgcat ttcggtgatc tcgatcacgc gctcctggag
    23641 cagcccgcct tccgccactt cctcctcggt gagccggcgg ttgaccggcc gccgctcgag
    23701 ccgcgtctcc tcgtggagca gctcgacatc ctcctgcacc gggatttcgg cgatatgggt
    23761 gtggacccgc gagcggccgc gcacgacctc gcgcttgccg atgcgcagct cttcctcgac
    23821 gatcgggatc cgctcctcct cctcgacggt cgccgcggcg gcggccgcca gcggtgccgc
    23881 caccgcttcg ggcgcctcca cccggcgctc cggcgcggga ggcgggggag gggccgcctc
    23941 ggccgcggga gccggagcgg gagcgggagc cgccgcccct tccggcgcac cgcctccgct
    24001 gatcccgccg agcaggcgca gcacggccgc gccgcggctc tcactggcga cctgcgccac
    24061 taacagatag ccgcccttcg ccacctgcgc ctcgcaggcg gaacgctcct cgggcgtcaa
    24121 ttgcaggctg tccagcgtca gcgcgccggc ggggccgctt ccgatcaccg ccgaatcgcg
    24181 caacggcacc tcggcggaga tggcggcgag ggcgcgctcg gcctcttcct cgctggcaaa
    24241 gcgggcggtg acggtcctcg gcatcagcct tcctcctcgg aatgtccggc tccggcaaca
    24301 tcgccgccgc cgccagtgcc gtcgcgttcg atcgacgcct gctcggagcg caaggtcacc
    24361 gcgacctcct cggtcgagct cgccgccttc cgccgcacat gtatctcctc gatcagcatc
    24421 agccgtttct cgaccaccac tacttcctcg accaccggga tgatggtggt gttgccttcg
    24481 agcctgacgc tgggcagctc ggacaagggg atattcttcg gcacccgctc gatcaccacc
    24541 tcgtcatggg tgagcgtgac cgggaccctt tcctcgcgct cgtcgaccga cacgcggacc
    24601 cgcagccgcc cggcctcgac
//
LOCUS       DASVRK010000242         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_483337, whole genome shotgun sequence.
ACCESSION   DASVRK010000242 DASVRK010000000
VERSION     DASVRK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483337"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VF589_09615"
     CDS             <1..678
                     /locus_tag="VF589_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257881.1"
                     /translation="LAGGGGDDSLSGLGGSDVLIGGAGTDRSDGGAGDDAHAVDSAAD
                     VVVERAGEGNDRVVAGADYGLRAGVHVEALAAAAGTAAIDLAGNELANRIDGNDGANI
                     LQGRGGADGLEGGGGADRFAFRAAEHAGLGAGRDTVLDFEGAGAAGGDRIDLASIDAV
                     QGGSDNAFALIGNAAFSAPGQLRVTYNPTLNVTTISGNTDADADAEFQIALTGNQVAL
                     NAADFVL"
     gene            complement(701..3886)
                     /locus_tag="VF589_09620"
     CDS             complement(701..3886)
                     /locus_tag="VF589_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257882.1"
                     /translation="MPVAHYPQREIVEVADFVREAVKGTASSEAYPDMVRLADGRHVL
                     VWSEPAYNDQPSGTWLQFLDSDGVPQGEPAKIGAGDAPVSVTATSDGFILSYRDGSLR
                     GFDGDGQAETGILDLADTKVDVATLADGRIVAAYVEDQVVKVQIFSEDLQPLGAPIAV
                     DGADLSRVEVAALGDGGFAVAYEDEGGRVSSRKYDADGAPVSGVVHHGASDAPEFSSG
                     RPSISASGAGGYAVVWTEASFHDGPHSISDIDEQVQLQLVGPDGTKVTGPILINENDT
                     GGFVIPTIRELADGGFYVTWGRSYLPDDVDYSGTNFDYGLAGRGFDSSGNPVLGEVML
                     SERGSIVEPLGAIGQAANGDIFVAFDFSDRSRDGSIELARLRQYQGSELLLGSEGKDN
                     LTGGEGRELLFGDGGDDTLAGGGDNDIVLGGDGADRLFGDDGNDRIDGGVGNDHLNGG
                     VGDDEIFGGAGNDRLFGGPGLDRLDGGTGSDTYVVSNRNTVVHDTGGGEDIIVARVDF
                     SLTSAIGVADLRLAGAAVTGEGNESANRLIGNGLANTLLGLAGGDSLAGEGGDDRLFG
                     NDGDDLLKGGAGMDRLNGGAGNDRLEGGEGDDILYVTQAGDSDDLNTLVGGAGNDRYV
                     IDSRVDRFEIVEEANGGIDRVVAYQNTVLAAEVEELELLGGATEGTGNALDNLIIGNA
                     GDNILNGAGGADRLVGGAGDDEYWVVDGLDTIEDSSGYDRVLAFRSFVLSDGLEELEL
                     QNGGDFNGTGNGSANRIEGNSGDNVLAGSGGDDRLIGRRGDDELRGGSGHDLLDGGVG
                     NDTLLGQSGIDTASYRAAAGAVTIDLAITIAQDSGGAGFDRIAGVENLVGSNFDDRLA
                     GGEVRNSLDGGTGDDTLIGRGGNDLLLGGDGSDDIRGGDGADQLIGGSQFDELNGEGG
                     ADVIRGGFDIDRLSGGAGDDRLFGNAGDDRIAGGEGLDQLMGGAGDDLFAFDDGDTGA
                     ARATADRVLDWDEGDRIDLSAVDADRSAGGDQAFSFIGNDAFGGTAGELRSQTIAGDL
                     YLQGDMDGDGNADLMVRVDDLAAITAADLIL"
     gene            4119..4325
                     /locus_tag="VF589_09625"
     CDS             4119..4325
                     /locus_tag="VF589_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257883.1"
                     /translation="MATAFKQIARLSGLLAIALLLVAAIVVAGFLFFLLLGTFGSAAS
                     LLVLAMLVTTGTVLFWLVVAAGNL"
     gene            complement(4353..4739)
                     /locus_tag="VF589_09630"
     CDS             complement(4353..4739)
                     /locus_tag="VF589_09630"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8257884.1"
                     /translation="MACHCTGCQRMTGSAFSLSSLFVSDGFRVTAGETVIGGLRGAAR
                     HHFCPHCMSWLFTRPEGMDDFVNVRSTMLDDPGSDPPFIETWTREKLPWAVTAATHSF
                     ETEPDPDRFPELLAAFADRARAQPPR"
     gene            complement(4807..5142)
                     /locus_tag="VF589_09635"
     CDS             complement(4807..5142)
                     /locus_tag="VF589_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8257885.1"
                     /translation="MLLTPEMCRAARALLNWKTPQLASASGLSIATVRRFEAGGMVRP
                     PSVDAMLNALQDAGLEFIPAGGKSLAGGPGIRTMPMPEPEVAAAEEALELEEPLGEAF
                     EDMQVESIV"
     gene            complement(5213..5380)
                     /locus_tag="VF589_09640"
     CDS             complement(5213..5380)
                     /locus_tag="VF589_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX8257886.1"
                     /translation="MGEFTDKMKGAANQAAGNLKQGSSNERVREEGAAQETKGEAQEV
                     KGKVKGVINKL"
     gene            complement(5476..>5731)
                     /locus_tag="VF589_09645"
     CDS             complement(5476..>5731)
                     /locus_tag="VF589_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX8257887.1"
                     /translation="IDQLSLSELFVRYQSIEGANAPGSAIGRGTVSSSSGGSTSTGGS
                     TGGTPVPAPAALWLFAAGIAGLGWQFGRKGSARLPEARFA"
BASE COUNT          905 a         1968 c         1864 g          994 t
ORIGIN      
        1 ctggcgggag gcggcggcga cgacagcctg agcggcctcg gcggcagcga cgtgctgatc
       61 ggcggcgccg gcaccgaccg cagcgacggc ggcgccggcg acgatgccca tgccgtcgac
      121 agcgcggccg acgtcgtcgt cgagcgggcg ggcgaaggca atgaccgggt cgtcgccggc
      181 gccgattacg gcctgcgcgc cggcgtccat gtcgaagccc tggccgcggc cgcgggaacg
      241 gcggcgatcg accttgccgg caacgaactc gccaaccgca tcgacggcaa cgatggggcc
      301 aacatcctcc agggccgcgg cggagcggac gggctggaag gcggcggcgg ggcggaccgg
      361 ttcgcatttc gcgcggccga gcatgccggc ctcggcgccg ggcgcgacac cgtcctcgat
      421 ttcgaagggg ccggcgcggc cggcggcgac cgcatcgact tggcttcgat cgacgccgtt
      481 cagggcgggt ccgacaatgc cttcgcgctg atcggcaacg ccgccttcag cgcgccgggc
      541 caattgcggg tgacctataa tccgaccctc aacgtcacca cgatctccgg caatacggac
      601 gcggacgccg acgccgaatt ccagatcgcg ctcaccggca accaggtggc gctgaacgcg
      661 gccgacttcg tcctgtagcg acgggtgcgg gcgcggcggc ttagaggatc agatcggccg
      721 cggtgatggc ggcgagatcg tcgacccgga ccatgaggtc ggcgttgccg tcgccatcca
      781 tgtcgccttg aagatacaga tcgccggcga tggtctggct gcggagttcg ccggcggtgc
      841 cgccgaaggc atcgttgccg atgaaggaga aagcctggtc gccgccggcc gatcgatcgg
      901 catcgaccgc gctgaggtcg atccggtcgc cctcgtccca gtcgagcacg cgatccgcgg
      961 tggcgcgggc ggcgccggta tcgccgtcgt cgaacgcgaa cagatcgtcg ccggccccac
     1021 ccatcagctg gtccaatcct tcgccgcccg cgatccggtc gtcgccggca ttgccgaaca
     1081 gccgatcatc cccggcgccg ccgctcagcc tatcgatgtc gaagccgccc cggatcacat
     1141 ccgcgccgcc ctcgccattg agctcgtcga attgcgagcc cccgatcagc tgatcggcac
     1201 cgtcgccccc gcggatatcg tcggagccat cgccgccgag cagcaggtcg ttgccgccac
     1261 gcccgatcag cgtgtcgtcg ccggtgccgc cgtccaggct gttcctcacc tcgccgcccg
     1321 ccaatctgtc gtcgaaattc gatcccacca gattttcgac gcccgcgatc cgatcgaagc
     1381 cggcgccgcc gctatcctgg gcgatggtga tcgcgagatc gatcgtgacc gcacccgccg
     1441 ccgcgcgata gctggcggtg tcgattccgc tctggccgag caaggtgtcg ttgccaacgc
     1501 cgccgtccag cagatcgtgg cccgagccgc cccgaagctc gtcatcgcct ctgcgtccga
     1561 tcagccgatc gtcgccgccg cttccagcga gcacattgtc gccgctattg ccttcgatgc
     1621 ggttggccga tccattgccg gtgccgttaa agtcaccgcc gttctgcagt tccagctctt
     1681 ccagcccgtc ggagaggacg aagctgcgga acgccagaac gcggtcatag ccgctcgagt
     1741 cctcgatcgt gtctaaacca tccaccaccc aatattcgtc gtcgccggct ccgccgacca
     1801 gccggtccgc gccgccagcg ccattcagaa tgttgtcacc cgcgttgccg atgatcaggt
     1861 tgtcgagcgc attgccggtg ccctcggtcg cgccgccgag cagctccagc tcttcgactt
     1921 cggcggccag gacagtgttc tggtaagcca ccacgcgatc tatgccgccg ttcgcctctt
     1981 cgacgatttc gaacctgtcc acgcgggagt cgatcacata gcgatcgttg ccggctcctc
     2041 ccaccaatgt attcagatca tcggaatcgc ccgcctgcgt cacgtagagg atgtcgtccc
     2101 cttccccgcc ctcgagacga tcattgccgg cgccgccgtt caggcggtcc atccccgcgc
     2161 cgcccttgag cagatcgtca ccgtcgttgc cgaacagccg gtcgtcgccg ccctcgcctg
     2221 ccaggctgtc gccgccggcc aggccgagca aagtattggc cagtccgtta ccgatcaggc
     2281 gattggccga ctcattgccc tcacccgtca ccgcggcgcc cgcaagccgc agatcggcga
     2341 ccccgatcgc cgatgtgagc gagaaatcca cgcgcgcgac gatgatatcc tcgccgccgc
     2401 ccgtgtcgtg cacgaccgta ttccgattgc tgacgacgta ggtgtccgag cccgtgccgc
     2461 cgtccaggcg gtcgagaccc ggcccaccga acagacggtc gttgccggcg ccgccgaaga
     2521 tctcgtcgtc gccgacgcct ccgttcaaat ggtcgttgcc aaccccgccg tcgatacgat
     2581 cgttgccgtc atcgccgaac aagcggtccg cgccgtctcc gccaagcacg atgtcattgt
     2641 cgccgcctcc ggcgagagtg tcgtcaccgc catctccgaa caggagttcc ctgccctcgc
     2701 cgccggtgag attgtccttg ccttcgctac ccagcagaag ctcgctgcct tgatattggc
     2761 gcagcctggc gagctcgatc gaaccgtccc tgctacggtc cgagaagtcg aaggcgacga
     2821 agatgtcgcc attggcagcc tgaccgatcg cgcccagcgg ttcgacgatg ctgccgcgct
     2881 cgctcagcat cacctcgcca aggaccggat tgcccgatga gtcgaagcca cgtccggcga
     2941 ggccataatc gaagttggtg ccggaatagt cgacatcgtc gggcaggtag ctgcgccccc
     3001 acgtcacgta gaaaccgccg tctgccaatt cccgaatagt ggggatgacg aaaccgcccg
     3061 tgtcgttctc gttgatgagg atcggcccgg tgactttcgt gccgtcgggc ccgacaagct
     3121 gcagctgaac ctgctcgtcg atgtcgctga tggagtgcgg gccatcgtgg aagctggcct
     3181 cggtccagac gacggcgtag ccgcctgccc cgctggccga aatcgagggc ctgccgctgg
     3241 aaaattccgg tgcgtcggac gcgccatgat ggaccactcc gctgacggga gcgccgtcag
     3301 catcgtattt gcgggacgag acccggccgc cctcatcctc ataggcgacg gcgaagccgc
     3361 catcgccgag cgccgctacc tcgacccggg aaaggtcagc tccatccacc gcaataggcg
     3421 caccgagcgg ttgcaggtct tccgaaaaaa tttgaacctt gacgacctga tcctcgacat
     3481 aggcggcaac gatgcgcccg tcggccaagg tggcgacatc gaccttcgtg tccgccagat
     3541 cgagtatgcc ggtttcagcc tggccatcgc cgtcgaagcc gcgcagggag ccgtcccggt
     3601 agctgaggat gaacccgtcg gacgtggccg tgaccgaaac cggcgcgtcg ccggccccga
     3661 tctttgccgg ctccccctgc ggaacgccgt cgctgtcaag aaactgaagc caggtcccac
     3721 ttggctgatc gttataagcc ggttccgacc agaccagcac atgccgcccg tcagccaggc
     3781 gcaccatgtc gggataggct tcgctgctgg cggttccttt caccgcttcg cgaacgaagt
     3841 cggcaacttc cacgatctcg cgctggggat aatgagcaac cggcatggga cctcggaatt
     3901 ttgagaaaag acgcttttat ccttgtgagt tatatggcgc tttgtcgatc cagcatgtgc
     3961 cggccagggt catccgttgg aggcggcggc tccggtctcc gctcgcgatc aggcactccc
     4021 ggcgagccgg gcgtgaaggt ccgagatcgg ggccgaatgt ccgcaatggc tggaagacga
     4081 accttcatcg aagctatggc ggtcgccgga ggcgtggcat ggcaacggcg ttcaagcaga
     4141 tcgcgcgatt atccgggctt ctggcgatcg ccttgctgct ggttgccgcc atcgttgtgg
     4201 ccggtttctt gttcttcctc ttgctcggga cgttcggctc ggctgcgtcg ctgctcgtcc
     4261 tggctatgct ggtgacaacc ggaaccgttt tattctggct cgtggtggcc gcgggcaatc
     4321 tctagcgctg cctcccagcg gttcggcggc actcagcggg gcggttgcgc gcgcgcccgg
     4381 tctgcgaaag cggcgagcaa ttccgggaag cggtcggggt cgggctcggt ctcgaagctg
     4441 tgggtcgcgg cggtgacggc ccagggcagt ttctcgcgcg tccaggtctc gatgaagggc
     4501 gggtcggagc cggggtcgtc cagcatggtc gagcggacgt tgacgaaatc gtccatcccc
     4561 tccgggcgcg tgaacagcca gctcatgcag tgcggacaga aatggtggcg ggcggcgccg
     4621 cgaaggccgc cgatcacggt ctcgccggcg gtgacgcgga agccgtcgct gacgaaaagc
     4681 gagctcaatg agaaggcgct gccggtcatg cgctgacagc ccgtgcagtg gcaggccatg
     4741 gtgacgaggg gcgcggatcc gatcgtaaag cggaccgccc cgcatcggca ttcgccctgg
     4801 atttcctcag acaattgatt ccacctgcat atcctcgaag gcttcgccca atggctcttc
     4861 gagctccagc gcctcttcgg cggccgccac ttcgggttcg ggcatcggca ttgttcggat
     4921 acccgggccg ccggccagcg acttaccgcc ggctgggatg aattcgagcc ccgcatcctg
     4981 gagcgcgttc agcatggcgt ccaccgaggg cggccggacc atgccgcccg cctcgaagcg
     5041 ccgcaccgtg gcgatgctca agcccgacgc ggaggcaagc tgaggcgtct tccagttgag
     5101 gagcgcgcgg gcggcgcggc acatttcggg ggtcaaaagc atggctgggg ttcgcatggc
     5161 cgagcggacc cggcaacata ttgccgagtc cgccgagacg ctttggaagc ggttagagct
     5221 tgttgatcac ccctttgacc ttgcccttca cttcctgggc ctcgcccttg gtttcctgcg
     5281 cggcgccctc ttcgcgtacg cgctcgttgc tgctgccctg cttgagattg ccggcggcct
     5341 ggttggcggc gcccttcatc ttgtcggtga actcacccat catctgtctc ctggttgatc
     5401 caggttagaa catgtcgggg cggcggtccg ttccaccgaa gcggcgcggg agccttggag
     5461 ccgcgtgccg ggggttcagg cgaagcgggc ttcgggcagg cgggccgagc ccttgcggcc
     5521 gaactgccag ccaagcccgg cgatgccggc ggcgaacagc cacaaggcgg ccggggcggg
     5581 aaccggggtt ccgccggtgc tgccgccggt gctggtgctg ccgccgctgc tggagctgac
     5641 ggtgccgcgt ccgatcgccg agccgggagc gttggcgccc tcgatcgact gatagcggac
     5701 gaacagctcg ctcagcgaaa gctggtcgat c
//
LOCUS       DASVRK010000243        16301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_422833, whole genome shotgun sequence.
ACCESSION   DASVRK010000243 DASVRK010000000
VERSION     DASVRK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16301
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422833"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..323
                     /locus_tag="VF589_09650"
     CDS             66..323
                     /locus_tag="VF589_09650"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX8257888.1"
                     /translation="MPDQLNQPDAIAQLSFEAALKRLEEIVRKLESGEAPLDESIELY
                     AEGDRLKQQCEARLQAAQARIEKIQFARDGSPSGTAPFDDV"
     gene            316..1230
                     /locus_tag="VF589_09655"
     CDS             316..1230
                     /locus_tag="VF589_09655"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX8257889.1"
                     /translation="MSDLTDRPAPEGLAAAMAEVGAEIDGMFSESLAVPPDARARLYE
                     AMRHAAIGGGKRLRPLLVRAACGLFAVDPARALRVGLAVECIHTYSLIHDDLPCMDDD
                     DLRRGKPTVHKAFGECTAILAGDSLHALAFEILADEATHEDPFVRAELVAELARAAGP
                     AGMAGGQMMDLIATETRPDLPAVTRLQQLKTGALIGFCLEAGAIMGRVPPEGRTRLRG
                     YARDVGLAFQIADDLLDAEGEEAKTGKRVGKDQQAGKETFVSLLGPERARRQAELLVA
                     QATEYLQSYGAEADLLRAIARFAVERDH"
     gene            1268..1771
                     /gene="coaD"
                     /locus_tag="VF589_09660"
     CDS             1268..1771
                     /gene="coaD"
                     /locus_tag="VF589_09660"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX8257890.1"
                     /translation="MTRTGVYPGTFDPVTLGHMDIIRRGAKLVDRLVIGVTTNPSKSP
                     MFTVEERIAMVRREIAEVEGAIDVVSFDSLLMDFAERQRASVIVRGLRAVADFEYEYQ
                     MAGMNQQLNDRIETVFLMADVSLQPIASRLVKEIAIYGGDIRKFVTPTVAEQVRARVD
                     EIGRKGS"
     gene            1895..2677
                     /locus_tag="VF589_09665"
     CDS             1895..2677
                     /locus_tag="VF589_09665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8257891.1"
                     /translation="MQFKTLFALVAGLTCAGSLAAQAPQRLPTSSKPVPAPAATSPLV
                     TPALLPENTLHLDLSSGGRVTIQLRPDHAPNYVQRVKTLTRQGFYNGIIFHRVIDGFM
                     AQTGDPTGTGTGGSPLPDVAAEFNPLPHVRGAVAAARATDVNSANSQFYIMLAPRLSL
                     DGEYTVIGRVVAGMAFVDAIQKGEPPANPSLIVRASIGSDNVPPLTPAELQAAAQAAA
                     QRRAAAAPAMIGQPPAATTGEAAPDTAEPATEKQPEAADTPQ"
     gene            2687..3715
                     /gene="queA"
                     /locus_tag="VF589_09670"
     CDS             2687..3715
                     /gene="queA"
                     /locus_tag="VF589_09670"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX8257892.1"
                     /translation="MRVELFDFELPPERIALRPASPRDSARMLVVRGGSLDDRSVLEL
                     PAQLRPGDLLVFNDTKVIPAQLEGRRGEARIGATLHKREGPRQWRAFVRNARRVREGD
                     AIDFGGGVVATASGRGEDGSWLLSFAGDEPVELLLERAGRMPLPPYIAARREADADDV
                     ADYQTMFAREQGAVAAPTAALHFTDRLMEALAAAGIGHATLTLHVGAGTFLPVKAEDT
                     DDHRMHSEWGRIDAVTADRLNAARAAGGRIVAVGTTSLRLLESAADAEGRVRPFEGDT
                     AIFITPGYRFRAVDGLLTNFHLPRSTLFMLVSALMGRESMQAAYAHAVARSYRFYSYG
                     DASLLLPG"
     gene            complement(3712..4107)
                     /locus_tag="VF589_09675"
     CDS             complement(3712..4107)
                     /locus_tag="VF589_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257893.1"
                     /translation="MRLALALALLATSSAGLASQAGDKKPFEEPGAREEWQEDQPLRP
                     GDKWIRVRMYVDKAGKPTKCRTIKSNIRSDSLRFQVCQAMMRDWHIEPVIENGVAVET
                     SVERMMIMPGPATRAAEEAARKRAKAQRR"
     gene            4212..5351
                     /gene="tgt"
                     /locus_tag="VF589_09680"
     CDS             4212..5351
                     /gene="tgt"
                     /locus_tag="VF589_09680"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX8257894.1"
                     /translation="MTCPRISFSVEATDGAARSGTLSMIRGDIRTPAFMPVGTAATVK
                     AMKPADVRASGADIILGNTYHLMLRPGAERVARLGGLHRFMGWDRPILTDSGGYQVMS
                     LADLRKLSEEGVSFKSHLDGSAHVLTPERSLEIQRLLGADIVMAFDECTPFPATCDEA
                     ASSMERSMRWAKRSRDAFDAGEAHASAAALFGIQQGSMAEDLRRISADKLTDIGFDGY
                     AVGGLAVGEGQPEMLRVLDFAVPMLPADRPRYLMGVGKPDDIVGAVERGIDMFDCVLP
                     TRSGRTGQAFTWTGPKNLKNARFAEDQGPLDPDCGCPVCATWSRAYIHHLIRSGEILG
                     AMLMTEHNLNFYQQLMAALRAAIAEGRLAAFAAAFRDRYGARAPT"
     gene            5463..7946
                     /locus_tag="VF589_09685"
     CDS             5463..7946
                     /locus_tag="VF589_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX8257895.1"
                     /translation="MTNPINVFTGYSDGDVDTNRSGNPTLESIVEARYSRRQTMMSGV
                     AALTTAALSTSFLAACSGGSNAEPATDLDVSAGANGRTSSGRPVNLTGSASAGADGYR
                     WEQVSGPAVELANANTPTASFIAPSVAESTPLVFRFTAEGAGASNNARTIVRVDPARL
                     GFEAVPKNKADRVTVPEGYTATVLYRTGDPIKPGVPNYRNNGTDSNFDGRAGDHHDAL
                     YYYGLSAAGGPDAAGNSRGLLVMNHENITQVYLHPNGPTPAPRPEAEALKEMEAHGVA
                     VVEVSRSGAGTWRYHRESALNRRITPLTEVDISGPARGSAQLKTAFSPDATRGRGTIN
                     NCANGYTEWSTYLTCEENWAFYFRRDSGDDAMRSPAELVSLGRYGIRQGNGGNYAWST
                     VQPQDANSNVYSRWNASVNAAAPADGSGDFRNEPNQFGWVVEIDPYDPAKAPRKRTAL
                     GRFAHEGAWPGRFVAGRRPAIYMGDDSRGEYLYKFVSATPWSADDAQAADRLATGDKY
                     LDQGTLYVARFEADGTGRWVPLVFGQGGLNPGNALYPFRDQADVLINARLAGDAVGAT
                     RMDRPEWTAINPHNGEMYLTLTNNNASLRPLTGTDPANPRHYNDPKTGEDGSVSPQVG
                     NPNGHIIRLREAGDTTEATSFRWDIYLFGAGADLNPANVNISGLDASNDFSSPDGLWF
                     ARPTNASGAVNPVLWIQTDDGAYTDVTNNQMLAAMPGQVGDGRPRRITNVGADGSMAA
                     QRTIVGRAPGANLRRFLVGPIEAEVTGVDTTPDGRTLFVNIQHPGEDGDLTELTSSWP
                     ATQSGESARARPRSATVVITRDDGGVVGL"
     gene            complement(7962..8534)
                     /locus_tag="VF589_09690"
     CDS             complement(7962..8534)
                     /locus_tag="VF589_09690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX8257896.1"
                     /translation="MTMITERTVLLPIDMQAAFDDPEWPRRWNKDVDRNGRALLAAWR
                     AAGRPIIHVRHDSVEPGSTLAPGTAGNAFRPGFEPLPGEPLVTKSVNSAFIGTDLDLR
                     LKRLRADKVVAFGITTDMCVSTTVRTGANMGWQMVLVPDACDCFELPDGRGGLIPAEQ
                     IHAAHVATLAFEFCELASLAELAQPQARAA"
     gene            complement(8597..10036)
                     /locus_tag="VF589_09695"
     CDS             complement(8597..10036)
                     /locus_tag="VF589_09695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8257897.1"
                     /translation="MTFADLGLSDELLRAVQESGYDVPTPIQRQAIPSVLMNRDIVGI
                     AQTGTGKTAGFVLPMIDILAQGRSRARMPRSLILEPTRELAMQVAENFEKYGKYHKLN
                     MALLIGGTNMADQIKKLERGVDVLIATPGRLMDLFGRGKIMLNGCGLLVIDEADRMLD
                     MGFIPDIEEICTKLPTTRQTLLFSATMPAPIKKLADKFLSNPKTIEVSRPASSNLLID
                     QRLIKVDARGKRDALGDLLRREEVTTAIVFSNRKTTVRELTTSLQRGGFKAGPIHGDM
                     EQPERIRELERFKAGEINILVASDVAARGLDIKGVSHVFNYDVPWQPDDYIHRIGRTG
                     RAGKTGTAITLATSADAEAIAAIEKLIGSKIPALEGSPQRTEEREQEARPAREESAAE
                     KPARRSREKQTAEPKARKRDETRPPREDRQPRTERAPREERQPEADRAPREEREPPRQ
                     ESGQAGEPALVDEGWNGPVPSFLGTGFGG"
     gene            complement(10136..10588)
                     /locus_tag="VF589_09700"
     CDS             complement(10136..10588)
                     /locus_tag="VF589_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257898.1"
                     /translation="MIDWSALALLLASIGGAEIEPSAHEPLQLAQVTIRRQIIVRVPV
                     RDGKTDPRASAPIGWKEKDGPRCVPAKLIVGASLLGQNSVDLLLRDNSRIRAKLESRC
                     PALDYYYGFYISPNADGQICADRDAIRSRVGGQCEIEGFKTLQAKHPR"
     gene            10688..12157
                     /locus_tag="VF589_09705"
     CDS             10688..12157
                     /locus_tag="VF589_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8257899.1"
                     /translation="MANPPPPALLEELERRLGPKGFTRAAADLAPWLEDWRKRYRGAA
                     AAMLSPADAGEVADIVRLCAGARVPLVPQGGNSSMAGGATPAPDGAALLLSLRRMNRI
                     RSISAGTNVAVAEAGVILTALHAAADAVGRRFPLTLGAKGSATIGGLVSTNAGGTQVL
                     RWGTMRGLVQGIEAVLPDGSLLSALTPLRKDNRGYDLRQLLIGAEGTLGVVTAATLRL
                     VPAIGSRTVAWVGLESPAEALDLLRLLENAVGDAVESFELVPRSALELVLRHVPGTRA
                     PLQGDHDWHVLVEAVAPMVAPSQEKALGRALAAALELGLIEDATIAASEAQAQAFWRI
                     RDSISEAEQKDGPAAKHDLSVEVAAMPGFMVEASAAVEGRFPGTRVIAFGHLGDGNVH
                     FNVRAPVGSGAEWLEREATGVTMFVHDLVTAAGGSISAEHGIGQMRLAELARLGDPAR
                     LAAMRAIKRALDPLGIMNPGKLVPASDVMAPPSQESLAE"
     gene            12233..13024
                     /locus_tag="VF589_09710"
     CDS             12233..13024
                     /locus_tag="VF589_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX8257900.1"
                     /translation="MATAAPTQQLPLFYHQLEPLSSNVHADYRARQVDNALFAVNAHA
                     VPITVDEFVSAQRFYPIVFSAGDSPVPLVLMGLNDGVNVFMGDDGKLLGEAYVPAYIR
                     RYPYMLARVRPEAQELSLCFDPTSGLVGPYDDGNALFEDGKPSETTKSVLKFCEEFEL
                     AAQRTTAFVNDLKEANLLMDGEVSLQPTGAEKPFLYRGFQMVSDEKLRDLRGDQLRKM
                     SQNGMLPLIHAHLFSLGIVREIFGKQVSQGKMPRQETGIPATADA"
     gene            complement(13190..14059)
                     /locus_tag="VF589_09715"
     CDS             complement(13190..14059)
                     /locus_tag="VF589_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX8257901.1"
                     /translation="MAESPAEPPFTRIGPENPASPVVLSVPHAGRAYSDALLRASRLP
                     RSKLEALEDRLVDRLVWRAVAAGAPALIASVPRAEIDLNRDEREIDAAMVAPPPPART
                     LMASPRTRGGLGLVPSRISGAGSIWLQRLPEAELAQRIETIHRPYHAALADMLEQARR
                     SFGVAILLDCHSMPPRSGGDAGGAAIVFGDRYGTSIRPDLLHAALAAAERSGFTTACN
                     APYAGGFITTRHGRPAAGIHALQIEIDRSLYLDRELREPGPGFDAASHLVAAVVAAVG
                     ARALDTRQAIAAE"
     gene            14211..14588
                     /locus_tag="VF589_09720"
     CDS             14211..14588
                     /locus_tag="VF589_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8257902.1"
                     /translation="MPKILLAEDDESMRLYLARALERVGYKVTTVDRGTAALPLLEAE
                     SFDLLLTDIVMPEMDGIELAQRAAVIAPEMRVMFITGFAAVALKAGSSAPTAKVLSKP
                     FHLRDLVAEVDRIFQSEDQHGRL"
     gene            14701..14775
                     /locus_tag="VF589_09725"
     tRNA            14701..14775
                     /locus_tag="VF589_09725"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14733..14735,aa:Val,seq:gac)
     gene            14943..>16301
                     /locus_tag="VF589_09730"
     CDS             14943..>16301
                     /locus_tag="VF589_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257903.1"
                     /translation="MGQGRAKQKARREREKLSKPISRSRFNLLAIGTRMSPAHYLYEE
                     VAYWGDLDERVIGVIARDNTDDDYYWSLLARDRNGRFRAADMDASLRSAGYATVALRE
                     RIAKAVTEDDLALLGTQGDETNQATDLLAVPPGTKPEYLHPNFKLLLDTPGRAPARVV
                     FKELGPWLAPSDPHFVKEFQTKHFDQRLWELYLWAVFRELGYDVTQPEAPDFHIVSPR
                     GEFTVEATTCAPSEGGVLADHPDPKTPEEMKAFIQDYMPMKFGNALTGKLNKKNAGGE
                     SYWQRGPGAGKPFVVAVADFHKPGGDGEIGSMTYTQEALWLYLYGSRIEWEFVDEKLT
                     IRTVKIDDHTYKTKTIETGFFNLEGAENISAVVFSNAGTLAKFDRMGVDAGFAPDGHR
                     YVRMGFRLDPDPNAVVGKTFIEEVVADNEERWSDELQVFHNPRAKFPLPLEAFAGATQ
                     HRF"
BASE COUNT         2614 a         5610 c         5465 g         2612 t
ORIGIN      
        1 acgagctgaa acgtcgcagt cgcagccgct tgacccccgc acccgccacc gccaatgctc
       61 ccgccatgcc cgaccagctc aaccagcccg acgccatcgc ccaactctcg ttcgaagccg
      121 ccttgaagcg gctcgaggag atcgtgcgca agcttgaaag cggcgaggcg ccgctcgacg
      181 agtcgatcga gctgtacgcg gagggcgacc gcctcaagca gcaatgcgag gcccggctcc
      241 aggcggccca ggcgcggatc gagaagatcc agttcgcccg cgacggcagc ccgagcggca
      301 ccgcgccctt cgacgatgtc tgacctcacc gaccgtccgg caccggaggg tctggcggcg
      361 gcgatggcgg aggtcggcgc cgagatcgac ggcatgtttt ccgaatcgct cgcggtgccg
      421 cccgatgcgc gcgcgcgcct ctacgaagcg atgcgccacg ccgcgatcgg cggcggcaag
      481 cggctgcggc cgctgctggt ccgcgccgcc tgcgggttgt tcgcggtcga tccggcgcgc
      541 gcgctccggg tcgggctggc ggtcgaatgc atccacactt actcgctgat ccacgacgat
      601 ctgccgtgca tggacgatga cgatcttcgc cgcggcaagc cgaccgtcca caaggcgttc
      661 ggcgaatgca ccgcgatcct ggccggcgat tcgctccacg cgctcgcgtt cgagatcctc
      721 gccgacgagg cgacgcacga ggatcccttc gtccgcgccg agctggtcgc ggagctcgcc
      781 cgcgcggccg ggcccgcggg catggccggc ggccagatga tggacctcat cgccaccgag
      841 acccggcccg atttgcccgc cgtcacccga ctgcagcagc tcaagaccgg ggcgctgatc
      901 ggcttctgcc tcgaagccgg ggcgatcatg ggccgcgtgc cgcccgaggg acggaccagg
      961 ttgcgcggct atgcgcgcga cgtcggcctc gccttccaga tcgccgacga cctcctcgat
     1021 gccgagggcg aggaggccaa gaccggcaag agggtcggca aggaccagca ggcgggcaag
     1081 gagacgttcg tgtcgctgct cgggccggag cgggcgcggc ggcaggcgga gctgctggtc
     1141 gcccaggcca ccgaatatct ccagagctac ggcgccgaag ccgacctgct ccgcgccatc
     1201 gcccgctttg ccgtcgagcg ggatcactga taaggacccg gccgggccgt caccgaaggg
     1261 ggatctcatg acacgcaccg gcgtctaccc gggcaccttc gatccggtca cgctcggcca
     1321 catggacatc atccgccgcg gtgccaagct ggtcgaccgg ctggtgatcg gcgtcaccac
     1381 caacccgtcc aaatcgccga tgttcaccgt cgaggagcgg atcgcgatgg tgcggcgcga
     1441 gatcgccgag gtcgaggggg cgatcgacgt ggtgagcttc gattcgctgc tgatggattt
     1501 cgccgagcgc cagcgcgcct cggtgatcgt ccgcgggctc cgggcggtcg ccgatttcga
     1561 atatgagtat cagatggcgg gcatgaacca gcagttgaac gaccgcatcg agacggtctt
     1621 cctgatggcc gacgtgtcgc tgcagccgat cgcctcgcgg ctggtcaagg agatcgcgat
     1681 ctacggcggc gacatacgca agttcgtcac gcccaccgtc gccgagcagg tgcgcgcgcg
     1741 ggtggacgag atcggccgca agggcagctg atcggccgcc ggctcatcga cggttcaggc
     1801 gcggcgatgg atggggcggc cgtccgcggc catgtttccg cttcgcgcgg aatgcggtta
     1861 aggtaagcgg gcaagattcg gggcgagggt ttcaatgcag ttcaagactt tgttcgcact
     1921 ggtggcgggg ttgacgtgcg ccggatcgct ggccgcacag gcgccgcagc gtctgccgac
     1981 gtcgagcaag ccggtgccgg cgccggccgc gacctcaccg ttggtgacgc ccgcgctcct
     2041 gcccgagaac acgcttcacc tcgacctttc gagcgggggc cgcgtgacga tccagctgcg
     2101 cccggaccat gcccccaatt acgttcagcg ggtcaaaacg ctgacccgcc agggcttcta
     2161 caacggcatc atcttccatc gggtaatcga cggcttcatg gcccagaccg gcgacccgac
     2221 cggcaccggc accggcggct ccccgctccc cgacgtcgcc gccgagttca acccgctgcc
     2281 gcatgtgcgc ggggcggtgg cggcggcgcg ggcgaccgac gtcaacagcg ccaacagcca
     2341 attctacatc atgctggccc cgcgcctctc gctcgacggc gaatataccg tgatcgggcg
     2401 ggtcgttgcc ggcatggctt tcgtcgacgc gatccagaag ggcgagccgc cggccaaccc
     2461 gtcgctgatc gtgcgggctt cgatcgggtc ggacaatgtg ccgccgctca ccccggccga
     2521 acttcaggcg gctgcgcagg cggctgccca gcgccgggcc gcggcggcgc cggcgatgat
     2581 cgggcagccg cccgccgcca cgaccggcga ggccgctccg gacaccgccg agccggccac
     2641 ggagaagcag ccggaggccg ccgacacgcc gcaatgaggc ggcgccatgc gcgttgagct
     2701 gttcgacttc gagctcccgc cggagcgaat cgcccttcgt ccggcgagcc cacgcgacag
     2761 cgcccggatg ctggtcgtgc gcggcggcag cctcgatgac cgaagcgtcc ttgagcttcc
     2821 cgctcagctc cggccgggcg acctgctcgt tttcaacgat accaaagtga tcccggctca
     2881 gctcgagggg cggcgcggcg aagcacggat cggcgccacg ctccacaagc gcgaggggcc
     2941 gcgccaatgg cgggcgttcg tccgcaatgc ccgccgcgtc cgcgaggggg atgcgatcga
     3001 tttcggcggc ggggttgtcg ccacggcttc cgggcgcggc gaagatggca gctggttgct
     3061 gagcttcgcc ggcgacgagc cggtcgagct attgctggag cgagcggggc ggatgccgct
     3121 gcctccctat atcgcagccc ggcgcgaggc cgatgcggac gatgtcgccg actatcagac
     3181 catgttcgct cgcgagcagg gagcggtcgc cgcgccgacg gccgcgcttc atttcaccga
     3241 ccggctgatg gaggcgctcg ccgcggccgg catcggccat gcgacgctta cgctccatgt
     3301 cggcgccggc accttccttc cggtcaaggc cgaggatacc gacgatcacc gcatgcatag
     3361 cgagtggggc cggatcgacg ccgtcaccgc cgatcgcctc aacgcagccc gcgctgccgg
     3421 cgggcgcatc gtcgccgtcg gcaccacctc gctgcggctg ctcgaaagcg ccgcggacgc
     3481 ggaggggagg gtgcggccgt tcgaggggga caccgccatc ttcatcacgc ccggctaccg
     3541 cttccgcgcg gtcgatggtc tcctcaccaa cttccatttg ccgcgctcga ccttgttcat
     3601 gctggtttcc gcgctgatgg gacgcgagtc catgcaggcg gcctatgccc acgccgtcgc
     3661 ccgcagctac cgcttctaca gctacggcga cgccagcctg ctgctgccgg gctagcggcg
     3721 ctgcgccttg gcgcgtttgc gggccgcttc ctcggccgcc cgggtggccg ggccgggcat
     3781 gatcatcatc cgctcgaccg aggtctcgac cgccacgcca ttttcgatca ccggctcgat
     3841 atgccagtcc cgcatcatcg cctggcagac ctggaaacga aggctgtcgc tgcggatatt
     3901 gctcttgatc gtccggcatt tggtgggctt gccggccttg tccacataca tgcgcacgcg
     3961 gatccatttg tcgccggggc ggagcggctg gtcttcctgc cattcctcgc gcgcgcccgg
     4021 ctcctcgaat ggcttcttgt ccccggcctg cgaggcgagg ccggcggagc ttgtggcgag
     4081 caaggcgagt gcaagtgcaa ggcgcatcga atatccctct gttccggcag cttatcagct
     4141 tcctttcagc gaacaagcgg ctcccggccc agccggcttc ctgcgcccca cttttccgct
     4201 agagagcccg catgacctgc ccccgcatct ccttttcggt cgaggcgacc gacggcgccg
     4261 cccgttcggg cacgctgtcc atgatccgcg gcgacatcag gacgcccgcc ttcatgccgg
     4321 tcggcaccgc cgccacggtg aaggcgatga agccggccga cgtccgcgca tcgggcgccg
     4381 acatcatcct cggcaacacc tatcacctga tgctgcggcc cggcgccgag cgggtggcgc
     4441 ggctcggcgg cctccatcgc ttcatgggct gggaccggcc gatcctcacc gacagcggcg
     4501 gctatcaggt gatgagcctc gccgatctgc gcaagctcag cgaggagggg gtgagcttca
     4561 aaagccatct cgacggctcg gcccatgtcc tgacgccgga gcggtcgctg gagatccagc
     4621 ggctgctcgg cgccgacatc gtgatggcgt tcgacgaatg cacgcccttc ccagcgacgt
     4681 gcgacgaggc cgcttcctcg atggagcggt cgatgcgctg ggcgaagcgc tcgcgcgacg
     4741 cgttcgacgc cggcgaggcg catgcctccg ccgccgcctt gttcggcatc cagcagggtt
     4801 caatggcgga ggatcttcgc cgcatctccg ccgataagct gaccgacatc ggcttcgacg
     4861 gctatgcggt cggcgggctc gcggtcggcg aggggcagcc ggagatgctg cgcgtgctcg
     4921 atttcgccgt gccgatgctg ccggccgacc gcccgcgcta tctgatgggg gtcggcaagc
     4981 ccgacgacat cgtcggcgcc gtcgagcgcg ggatcgacat gttcgactgc gtccttccca
     5041 cccgctccgg ccgcaccggc caggccttca cctggaccgg cccgaagaac ctcaagaacg
     5101 cgcggttcgc cgaggaccag gggccgctcg accccgattg cggctgcccg gtctgcgcga
     5161 cctggagccg cgcctatatc caccatctca tccgctcggg cgagatactt ggcgcgatgc
     5221 tgatgaccga gcacaacctc aatttctatc agcagctgat ggccgctttg cgcgccgcga
     5281 tagccgaggg ccggctcgcc gccttcgccg cggccttcag ggaccgttat ggggcacggg
     5341 cgccaacata aacgtaacat atccgtatcg cggctgacgc cgaactgatc cggcttcgcc
     5401 acaaccctcc gtcatggctg tgctaacgcg attcttcgcg gccacgcttt gagggggaaa
     5461 gaatgaccaa tccaatcaac gtcttcaccg gctattcgga cggcgacgtc gacaccaacc
     5521 gctccggcaa cccaaccctc gaaagcatcg tcgaagcccg ctattcccgc cgccagacta
     5581 tgatgagcgg cgtcgccgcc ctcaccaccg ccgccctctc caccagcttc ctcgccgcct
     5641 gcagcggcgg cagcaacgcc gagcccgcca ccgacctcga cgtcagcgcc ggcgccaacg
     5701 gccgcaccag ctccggtcgc ccggtcaacc tcaccggaag cgccagtgcg ggcgccgacg
     5761 gctatcgctg ggagcaggta tcgggtcccg ccgtcgagct cgccaacgcc aacacgccga
     5821 ccgccagctt catcgccccc agcgttgccg aatccacccc gctcgtcttc cgcttcaccg
     5881 ccgagggagc gggggcttcc aacaatgccc gcaccattgt ccgggtcgat ccggcgcggc
     5941 tcggcttcga agccgtgccc aagaacaagg ccgaccgcgt caccgtgccc gaaggctaca
     6001 ccgccaccgt gctctaccgc accggcgacc cgatcaagcc gggcgtcccc aattaccgca
     6061 acaacggcac cgactcgaat ttcgacggcc gcgccggcga ccatcatgac gcgctttatt
     6121 attatggcct gtccgccgcc ggcggccccg acgcggccgg taacagccgc gggctgctgg
     6181 tgatgaacca tgagaatatc acgcaggtct acctccaccc gaacggccca acgcccgccc
     6241 cgcggcccga ggcggaggcg ctgaaggaaa tggaagcgca tggcgtcgcc gtcgtcgagg
     6301 tgagccgcag cggcgcgggg acgtggcgct accatcgcga atcggcgctc aaccggcgca
     6361 tcacgccgct gaccgaagtc gatatcagcg gccccgcccg gggaagtgcg cagctgaaga
     6421 cagccttctc gcccgacgcc acgcgcggcc gcggcaccat caacaattgc gccaacggct
     6481 ataccgaatg gagcacctac ctcacctgcg aggagaattg ggccttctac ttccgccgcg
     6541 actccggcga cgacgccatg cggagcccgg ccgagctggt atcgctgggc cgctacggca
     6601 tccgccaggg caatggcggc aattacgcct ggagcaccgt ccagccgcag gatgcgaaca
     6661 gcaatgtcta ttcgcgctgg aacgcttcgg tgaacgcggc cgcgccggcc gacggcagcg
     6721 gcgacttccg caacgaaccc aaccagttcg gctgggtcgt cgagatcgat ccttatgatc
     6781 cggccaaggc accgcgcaag cgcaccgcgc tcggccgatt cgcgcatgag ggcgcttggc
     6841 ccggccgctt cgtcgccggc cgtcgccccg caatctatat gggcgacgat tcccgcgggg
     6901 aatatctgta caagttcgtc tcggccacgc cctggtccgc ggacgacgcg caggcggccg
     6961 accggctggc aaccggtgac aaatatctcg accagggcac gctttacgtc gcccgcttcg
     7021 aagcggacgg caccggccgc tgggtgcccc ttgtgttcgg tcagggcgga ttgaaccccg
     7081 gcaatgccct ctatccgttc cgggaccagg ccgacgtgct gatcaatgcg cggctcgcgg
     7141 gcgacgccgt cggcgcgacc aggatggacc ggccggaatg gaccgcgatc aatccgcaca
     7201 atggcgagat gtacctgacc ctcaccaaca acaacgcctc gttgcgcccg ctcaccggca
     7261 ccgacccggc caacccgcgc cactataacg atccaaagac gggcgaggac ggctcggtct
     7321 cgccgcaggt cggcaatccc aacggccaca tcatccggct tcgcgaggcg ggcgacacga
     7381 ccgaggcaac cagcttccgc tgggacatct acctgttcgg cgcgggcgcc gacctcaacc
     7441 ccgccaacgt caacatctcc ggcctcgacg ccagcaacga cttttcgagc cccgacgggc
     7501 tgtggttcgc gcggccgacc aacgcctccg gcgccgtcaa cccggtgctt tggatccaga
     7561 ccgacgacgg cgcctatacc gacgtcacca acaaccagat gctggcggcg atgccggggc
     7621 aggtcggcga tggccgtccg aggcggatca cgaatgtcgg cgcggacggc tcgatggcgg
     7681 cgcaacgcac gatcgtgggg cgggcgccgg gcgccaacct caggcgcttc ctggtggggc
     7741 cgatcgaggc cgaagtcacc ggcgtcgaca ccacgccgga cggccgtacc ttgttcgtca
     7801 acatccagca tcccggcgag gacggcgacc tcaccgagct caccagcagc tggccggcca
     7861 cccagagcgg cgaatccgcg cgggcgcggc cgcgctcggc gaccgtggtc atcacccgcg
     7921 acgatggcgg tgtggtgggg ctctgagcgg cgaaaccagc ctcaggcagc ccgtgcctga
     7981 ggctgggcga gctccgcgag cgaggccagt tcgcagaatt cgaacgcgag cgtcgcgacg
     8041 tgggcggcgt ggatctgctc ggccgggatc aggccgccgc ggccgtcggg tagctcgaag
     8101 cagtcgcagg cgtcggggac cagcaccatc tgccagccca tattggcgcc ggtgcgcacg
     8161 gtggtcgaaa cgcacatgtc ggtggtgatc ccgaacgcca ccactttatc ggcgcggagc
     8221 cgcttcagcc gcaggtcgag gtcagtgccg atgaaggccg agttgacgct cttggtgacg
     8281 agcggctcgc cgggcagcgg ctcgaagccg gggcggaagg cgttgccggc agtgcccggt
     8341 gccagcgtgg agccgggctc gaccgaatcg tgccgcacgt ggatgatcgg ccggcccgcg
     8401 gcgcgccagg cggcgagcag ggcgcggccg ttgcggtcca catccttgtt ccagcggcgc
     8461 ggccattcag ggtcgtcgaa cgcggcttgc atgtcgatcg gcagcaacac ggtccgctcg
     8521 gtgatcatgg tcattgaact tctccccgtg atcgatcggc actcggctga gcggctcgag
     8581 acgtgacccg ctgctgttag ccgccgaagc ctgtgcccaa gaagctcggc accgggccgt
     8641 tccagccttc gtcgaccagc gccggctccc cggcttgccc gctctcctgc cggggcggct
     8701 cccgctcctc acgcggcgcg cgatccgctt cgggctggcg ctcctcgcgc ggcgcgcgct
     8761 ccgttctggg ctggcgatcc tcacgaggcg gcctggtttc gtcgcgcttg cgcgccttcg
     8821 gctccgccgt ctgcttctcg cgcgaccgcc gggcaggttt ctctgccgcc gattcctcgc
     8881 gcgcgggtct ggcctcttgc tcgcgttctt cggtgcgctg gggcgagcct tcaagcgccg
     8941 ggatcttgga tccgatcagc ttctcgatcg cggcgatcgc ctcggcgtcg gcgctggtgg
     9001 cgagggtgat cgcggtgccg gtcttgccgg cgcggccggt gcggccgatc cggtggatat
     9061 aatcgtccgg ctgccacggc acgtcatagt tgaagacgtg gctgacgccc ttgatgtcga
     9121 gcccgcgcgc ggcgacgtcg gaggcgacca ggatgttgat ctcgccggcc ttgaaccgct
     9181 cgagctcgcg gatccgctcc ggctgctcca tgtcgccgtg gatcggcccg gccttgaagc
     9241 cgccgcgctg gaggctggtg gtgagctcgc ggaccgtcgt cttgcggttg gagaagacga
     9301 tcgcggtggt gacttcctcg cgccgcagaa ggtcgccgag cgcatcgcgc ttgccgcgcg
     9361 cgtccacctt gatcagccgc tggtcgatca gcaggttgga ggaggccggg cgggacacct
     9421 cgatcgtctt cgggttcgac aggaacttgt cggccagctt cttgatcggc gccggcatgg
     9481 tcgccgagaa gagcaatgtc tgccgcgtcg tcggcagctt ggtgcagatc tcctcgatgt
     9541 ccgggatgaa gcccatgtcg agcatgcggt cggcctcgtc gatgaccagc aggccgcagc
     9601 cgttgagcat gatcttgccg cgtccgaaca ggtccatcag ccgccccggc gtcgcgatca
     9661 gcacgtcgac gccgcgctcc agcttcttga tctggtcggc catgttggtg ccgccgatca
     9721 gcagcgccat gttgagcttg tggtacttgc cgtacttctc gaaattctcc gccacctgca
     9781 tcgccagctc gcgggtgggc tcgaggatca gcgagcgcgg catgcgggcg cggctccggc
     9841 cttgggcgag gatgtcgatc atcggcagca cgaaaccggc ggtcttgccg gtgccggtct
     9901 gggcgatgcc gacgatgtcg cggttcatca gaaccgatgg gatcgcctgg cgctggatcg
     9961 gggtcggcac gtcatagccc gattcctgca ccgcgcggag taattcgtct gaaaggccga
    10021 ggtcggcaaa ggtcataagg attccggaaa attgcgtctt gaagtgaagc cgctttgccc
    10081 attcgcttgg gcgaagcgat acgcccctgt caaggcgggg ccggtcgagg ctgggtcagc
    10141 ggggatgctt cgcctgcagg gttttgaagc cttcaatctc gcattgcccc cccacccggg
    10201 aacggatggc gtcgcgatcg gcgcagatct gcccgtcggc gttggggctg atgtaaaaac
    10261 cgtaataata atccagcgcc gggcagcggc tctcgagctt ggctcgaatc cggctgttgt
    10321 cgcggagcag gagatccacg ctattctgtc ccagcaggga cgcgccgacg atcagcttcg
    10381 cgggcacgca gcgcgggccg tctttctcct tccagccgat cggagccgag gcgcgcgggt
    10441 cggtcttgcc atcgcgcacc ggcacccgga cgatgatctg gcggcgaatc gtcacctgcg
    10501 cgagctggag cggctcgtgc gcggaaggtt caatctccgc tccgccgata ctcgccagca
    10561 aaagcgccag cgcgctccaa tcgatcaaag tcccggctcc atcgcctacc gccatccgag
    10621 ctagcgcttg aacatgcgat taactttttg ctcgccccga tgccagctag taggcgctcc
    10681 gaagcatatg gccaatcctc ccccacccgc tctgttggaa gagcttgaac gccggctcgg
    10741 cccgaagggg ttcacccgcg ccgccgcgga tctcgcgccg tggctcgagg attggcggaa
    10801 gcgctaccgc ggtgcggccg cagccatgct gtcgccggcc gatgccggag aagtggccga
    10861 catagtccgg ctctgcgcgg gtgcgcgcgt gccgctggtg cctcagggcg gcaacagctc
    10921 tatggcggga ggggcgacac cggctccgga cggcgctgct ttgctcctgt cgctgcggcg
    10981 catgaatcgg atacgatcga tctcggcagg caccaacgtt gccgtcgcgg aggcgggcgt
    11041 gatcctcacg gcgctgcacg cggctgccga tgccgtgggc cgccgctttc ctctgacgct
    11101 cggggcaaaa gggagcgcca cgatcggcgg tctcgtctcg accaacgccg gagggacgca
    11161 ggtcctccgc tggggcacca tgcgcggatt ggtccagggc atcgaagccg tgctcccgga
    11221 cggatcgttg ctgagcgcgc tgacgccgct caggaaagat aatcgcggct acgatttgcg
    11281 gcagttgctg attggcgccg agggtacgct cggtgtggtt accgcggcga ctctgaggct
    11341 ggtcccggcg atcggttcgc gcaccgtggc gtgggtgggg ctcgagtctc cggccgaggc
    11401 gctcgacctt ctccggctac tcgaaaatgc cgtcggcgac gcggtggaaa gcttcgaact
    11461 ggtgccgcgc tcggcgctcg aactggtcct ccgccacgtc ccgggaaccc gcgcccccct
    11521 tcagggcgat catgactggc acgttctcgt cgaagcagtg gcgccgatgg tcgcgcccag
    11581 ccaggaaaag gcgctcggac gggctctcgc cgccgcgctc gagcttggtt tgatcgagga
    11641 tgcgaccatc gcggccagcg aggctcaggc gcaggccttt tggcgcatcc gcgacagcat
    11701 ctccgaagct gagcagaaag acggtcccgc ggcaaagcac gacctctccg tcgaagtggc
    11761 ggcgatgccc ggcttcatgg tcgaggcctc cgcggcggtc gaaggccggt ttccggggac
    11821 gcgggtgatc gccttcggcc atctcggcga cggcaacgtc catttcaacg ttcgggcgcc
    11881 ggtcgggagc ggcgcggaat ggctcgagcg ggaggcgacc ggcgtcacca tgttcgtcca
    11941 tgatctcgtc accgcggccg gcgggtcgat ctccgccgag cacggcatcg gccagatgcg
    12001 cctggcggag cttgcgcgac tcggcgatcc tgcccggctg gccgcgatgc gcgcgatcaa
    12061 gcgggcgctc gacccgctcg ggatcatgaa ccccggtaag ctggtcccag cctccgacgt
    12121 aatggcgccg ccatctcagg agagccttgc ggaatagccg cttcacccat agacggtggc
    12181 gctgaaaccg gcggccgtct gggctgcttc atcaacttgc cggagaatca tcatggcgac
    12241 cgcagcgccg acgcaacaat tgccgctttt ctaccatcag ctcgagccgc tctcgagcaa
    12301 tgtccacgcc gactatcggg cgcgccaggt cgataacgct ctgttcgccg tcaatgccca
    12361 tgctgtgccg atcacggtcg acgagttcgt ctcggcccag cgtttctatc cgatcgtctt
    12421 ctcggctggg gatagtccgg tgccgttggt gttgatgggc ctcaacgacg gtgtcaacgt
    12481 gttcatgggc gacgacggca agctgctcgg cgaggcctat gtgccggcct atatccgccg
    12541 ctacccctat atgctcgccc gcgtccgccc cgaggcccag gaattgtcgc tctgcttcga
    12601 tccgaccagc ggcctggtcg gtccctatga cgacggcaat gcgctgttcg aggacggcaa
    12661 gcccagcgag accaccaaga gcgtgctcaa attctgcgag gagttcgagc tcgccgccca
    12721 gcgcaccacc gccttcgtca acgacctcaa ggaagccaat ctgctgatgg acggcgaagt
    12781 ctcgctgcag ccgaccggcg ccgaaaagcc gttcctctat cgcggcttcc agatggtctc
    12841 cgacgagaag ctgcgcgacc tgcgcggcga ccagctccgc aagatgagcc agaacggcat
    12901 gctgccgctg atccacgcgc atctcttctc gctcggcatc gtccgcgaga ttttcggcaa
    12961 gcaggtttcg caaggcaaaa tgcccaggca ggagacgggg ataccggcca ccgccgacgc
    13021 ctgatcgggg cgcgcagaat ctccttttga tccgtcttga aaggcggcgc ggccgcccta
    13081 tctaggatcc gaccgctgcc gtttcccccc tttacggcag tgcgttacgc ccttttgggc
    13141 gttcctccct gaaccttggc caccccgaat tttcggggtg gtcttttttt tactcggccg
    13201 cgatcgcctg gcgcgtgtcg agcgcccgcg cgccgaccgc ggcgacgacc gcggcgacga
    13261 ggtgcgaggc ggcgtcgaag ccgggcccgg gctcgcgcag ctcccgatcg aggtagagcg
    13321 agcggtcgat ctcgatctgc agcgcatgga tgcccgcagc cgggcggccg tggcgggtgg
    13381 tgatgaagcc gcccgcataa ggcgcgttgc aggcggtggt gaagccggac cgctcggcgg
    13441 cggcgagcgc ggcgtggagg aggtcgggcc ggatgctggt gccgtagcgg tcgccgaaca
    13501 cgatcgcggc gccgccggcg tcgccgccgc tgcgcggcgg catcgaatgg caatcgagca
    13561 ggatcgcgac gccgaagctc cgccgcgcct gctccagcat gtcggcgagc gcggcatgat
    13621 aggggcggtg gatggtctcg atgcgctggg cgagctccgc ctccggcagc cgctgcagcc
    13681 agatcgaccc ggcgccgctg atccgcgacg gcaccagccc gagcccaccc cgggtccgcg
    13741 gcgacgccat cagggtgcgc gccggcggcg gcggcgccac catcgcggcg tcgatctcgc
    13801 gctcgtcccg gttgagatcg atctcggccc gcggcacgct cgcgatcagc gccggcgcgc
    13861 cggccgcgac cgcgcgccag accaggcggt cgaccagccg gtcctcgagc gcctcgagct
    13921 tcgagcgcgg cagccgcgag gcgcggagca aggcgtccga ataagcgcgg ccggcatggg
    13981 gcaccgaaag caccaccggc gaggcgggat tttcggggcc gatgcgcgtg aacggcggct
    14041 cggcggggga ttcggccatt agcaatgaaa ctagggagcg tcccccgatg gcgcaatcgc
    14101 cttccggcag gcggggcggc gctcgcggcg gctggaaaag gcatgccgct tagggcataa
    14161 gcgaaaggcg gcggcgaaca gcgcgtcgcc tagcctgtag cggagcgcgg atgccgaaaa
    14221 tcctcctggc cgaagatgac gagtcgatgc ggctctatct ggcgcgcgcg cttgagcggg
    14281 tcggctacaa ggtcaccacc gtcgaccgcg gcaccgcggc gctgccgctg ctcgaggcgg
    14341 agagcttcga cctgctgctg accgacatcg tcatgcccga aatggacggc atcgagctcg
    14401 cccagcgggc ggcggtgatc gcgcccgaga tgcgggtgat gttcatcacc ggcttcgccg
    14461 cggtggcgct caaggccggc agcagcgcgc ccaccgccaa ggtgctgtcg aagccgttcc
    14521 acctgcgcga cctggtcgcc gaggtcgacc gcatcttcca gtccgaggac cagcacggcc
    14581 gcctctgagc gcggggggcc ggcgcggtgc gccgaccggg tccaccgaag cgggagcgag
    14641 gggaagcacg ggcaaagcct gcttgcgccc ccgaaaaggc ccggctaaag gcgcggtcgt
    14701 gggcgtgtag ctcagtggta gagcactgtg ttgacatcgc aggggtcgca agttcaatcc
    14761 ttgccacgcc caccattttt tattggctga gtgaagtggc gccgcgttag gcggtgcggc
    14821 tcgtttgtac tccggtgcaa ttccggtgac ggcacctgga acgagagctt cgatgccccg
    14881 cgatgatcgt ttcgatcgcc tgccgcgcca ccggcgcgac agcatccagg atggagttct
    14941 aagtgggcca aggaagggcg aagcagaagg caaggcgcga gcgggagaag ctcagcaagc
    15001 ccatttcacg atcgcggttc aacctcctcg ccatcggaac gcgcatgtcc ccagcgcact
    15061 acctctacga agaggtcgcc tactggggcg accttgatga gcgtgtgatt ggcgtcatcg
    15121 cgcgggataa taccgacgac gactactatt ggtcgctgct cgcccgggac cgcaatggga
    15181 ggttccgcgc ggccgatatg gacgcgagtc tacgctccgc tggctacgcg acagtggcgc
    15241 ttcgagaacg gatcgcgaag gccgtcacgg aggacgacct cgcgttgctt ggcacacagg
    15301 gcgacgagac gaaccaggcc accgacctcc tcgcggttcc acccggcacg aagccggaat
    15361 acctgcaccc gaacttcaag ctgctgttgg acactccagg ccgcgcacct gcccgcgttg
    15421 tgttcaagga gctggggccg tggctcgcgc cgagcgatcc gcacttcgtt aaggagttcc
    15481 agaccaagca ttttgaccaa cgcttgtggg agctttacct ctgggcggtc ttccgggagc
    15541 tcggctacga cgtgacgcag ccggaggccc ccgatttcca catcgtgtcg ccccggggag
    15601 agttcacggt ggaggcaacc acgtgcgcgc cgtcggaggg aggcgtgctt gccgaccatc
    15661 ccgatccgaa gacgccagag gagatgaagg ccttcattca agactacatg ccgatgaagt
    15721 tcggcaatgc gttaacaggc aagctcaaca agaagaacgc tggcggcgag agctactggc
    15781 aacggggacc cggagcgggg aagcccttcg tcgtagcagt cgctgacttc cacaagccgg
    15841 gcggcgacgg cgagattggc tcgatgactt atactcagga ggcgctgtgg ctttacctgt
    15901 atggcagccg gatcgagtgg gagttcgtcg acgagaagct gaccatccgc actgtgaaaa
    15961 tcgacgacca tacgtacaag acgaagacaa ttgagacagg cttctttaac ctggaaggtg
    16021 cggagaacat ctcggccgtc gtgttttcga acgcgggaac gctcgcgaag ttcgatcgga
    16081 tgggggtcga cgccggcttt gctcccgacg gccaccgcta cgttaggatg ggcttccgcc
    16141 tcgaccccga cccgaacgcc gtcgtgggaa agaccttcat cgaggaggtc gtcgctgaca
    16201 atgaggagcg ttggtccgac gaactccagg tgttccacaa ccctcgcgca aagttccccc
    16261 ttccgctaga agcattcgcc ggcgccaccc agcaccggtt c
//
LOCUS       DASVRK010000244         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_483827, whole genome shotgun sequence.
ACCESSION   DASVRK010000244 DASVRK010000000
VERSION     DASVRK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483827"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF589_09735"
     CDS             complement(<1..244)
                     /locus_tag="VF589_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257904.1"
                     /translation="MEGEPVEALQPVERGLLRVMRIQALAAATILLGAGAVGEAVLRD
                     RTGFPPGLILAPLLLLLAYLVLVVPGRRYRALGYRFD"
     gene            complement(249..935)
                     /gene="thiE"
                     /locus_tag="VF589_09740"
     CDS             complement(249..935)
                     /gene="thiE"
                     /locus_tag="VF589_09740"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX8257905.1"
                     /translation="MTDEEEALALDPDFPHLFDRGERAPCQLYLVSPLEVGGDFAERL
                     AAALDRGPVAAFQFRVKGVDQHQAARLAEPLQRICADREIAFIVNDDMGLAKRIGADG
                     VHLGQGDGDPREARALLGPTAQLGITCHASRHLAMEAGEAGADYVAFGAFYPTSTKET
                     LHRPEPSILGWWSRLFEIPCVAIGGITPANAAPLVEAGADFLAVCGSVWNVPEGPGAA
                     VGAFADLLRG"
     gene            complement(932..1546)
                     /locus_tag="VF589_09745"
     CDS             complement(932..1546)
                     /locus_tag="VF589_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8257906.1"
                     /translation="MIRRFLARQFARPSGWVGRWWIGPWLNRIGRAMNRLALEQLELR
                     PGDDVLEVGFGGGELLGRILAATSGEVFAVDISAAMVERARRRFGGKERLHLHRASAA
                     GLPIADGAVDKACSVNALYFWEDPAVPLRELARVLRPGGRLVLCFQSPEAVRAWPGHV
                     HGFAAYEVEAIIGHMEQAGFGPPRVTHGSHASVGDFVCLTSERT"
     gene            complement(1554..>1916)
                     /locus_tag="VF589_09750"
     CDS             complement(1554..>1916)
                     /locus_tag="VF589_09750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053630.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX8257907.1"
                     /translation="EVNIKSPERAEADRILLDELTKALDALPGDDKVMLKLSIPAEAG
                     LFQPLVDHPRVLRVVALSGGYSREEACGELARNPGMIASFSRALLEDLRHQMTDREFD
                     EALGKAIDEIYQASTVKS"
BASE COUNT          315 a          709 c          636 g          256 t
ORIGIN      
        1 catcgaagcg atagccgagt gcgcggtagc gccgcccggg cacgaccagc acgagataag
       61 cgagcagcag cagcagcggc gccaggatca ggcccggggg aaagccggtg cggtcgcgca
      121 ggaccgcctc gccgaccgcg cccgccccca gcaggatggt ggcggccgcc aaagcttgga
      181 tccgcatcac ccggagcagg ccgcgctcga ccggctgcaa cgcctccacc ggctcgccct
      241 ccaccggctc agccccgcag cagatcggcg aaggcgccga ccgcggcgcc aggcccttcc
      301 ggcacgttcc agacggaccc gcacacggcc aggaaatcgg cgcctgcctc caccaaaggc
      361 gcggcgttgg ccggcgtgat cccgccgatc gccacgcagg gtatctcgaa cagccgcgac
      421 caccagccga ggatcgacgg ctcaggccgg tggagggtct ccttggtcga ggtcgggtaa
      481 aaagcgccga aggcgacata atcggcgccg gcctcgcccg cctccatggc gaggtggcgg
      541 ctggcatggc aggtgatgcc gagctgcgcc gtcggcccga gcagcgctcg cgcctcgcgc
      601 gggtcgccat cgccctggcc gagatggacg ccgtcggcgc cgatccgctt ggcgaggccc
      661 atgtcgtcgt tgacgatgaa cgcgatctcg cgatccgcgc aaatgcgctg gagcggttcg
      721 gcgagccgcg ccgcctgatg ctgatccacg cccttcaccc ggaactggaa cgcggccacc
      781 ggccccctgt cgagcgccgc cgctagccgc tcggcgaaat cgccgccgac ttcgagcggc
      841 gagacgaggt aaagctggca cggcgcccgc tcgccgcggt cgaacaagtg cgggaaatcg
      901 ggatcgagcg cgagcgcctc ttcctcgtcg gtcatgtccg ctcgctggtc aggcacacaa
      961 aatctccgac actggcatgg ctgccatggg tcacacgcgg cgggccgaat cccgcttgct
     1021 ccatatgccc tattatcgct tcgacttcat aagcggcaaa tccgtggaca tggccgggcc
     1081 aggcgcggac cgcctcgggc gactggaagc acaggacgag ccgcccgccc ggccgcagta
     1141 cgcgcgccag ctcgcgcaac ggaacggccg gatcctccca gaaatagagg gcattgacgc
     1201 tgcaggcctt gtcgacggcg ccgtctgcga tgggcaggcc cgcggcggag gcgcggtgga
     1261 gatggagccg ctccttcccg ccgaaccggc gccgcgcccg ctccaccatc gccgccgaga
     1321 tgtccaccgc gaacacctcc cctgacgtcg ccgccaggat ccggccgagc aattcgccgc
     1381 cgccgaaacc tacctccagc acatcgtcgc ccggccgtag ctcgagctgt tccagcgcca
     1441 gccggttcat cgcccggccg atgcggttca gccacggccc gatccaccag cgtcctaccc
     1501 acccgctcgg ccgcgcaaac tgccgggcga ggaagcggcg gatcacgccc gccctagctc
     1561 ttgacggtcg aggcctgata gatttcgtcg atcgccttgc cgagcgcctc gtcgaactcg
     1621 cggtcggtca tctggtggcg cagatcctcg agcaatgcgc gcgagaagct ggcgatcatg
     1681 cccggattcc gtgcaagctc gccgcacgcc tcctcgcgcg aatagccgcc cgacagcgcg
     1741 accacgcgca gcacgcgggg atggtcgacc agcggctgga acaggcctgc ctcggccggg
     1801 atcgagagct tcagcatgac cttgtcgtcg ccggggagcg cgtcgagcgc cttggtgagc
     1861 tcgtcgagca ggatccggtc agcctcggcc cgctcggggc tcttgatgtt gacctc
//
LOCUS       DASVRK010000245         5521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_359443, whole genome shotgun sequence.
ACCESSION   DASVRK010000245 DASVRK010000000
VERSION     DASVRK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359443"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..100
                     /locus_tag="VF589_09755"
     tRNA            24..100
                     /locus_tag="VF589_09755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Arg,seq:cct)
     gene            124..1062
                     /locus_tag="VF589_09760"
     CDS             124..1062
                     /locus_tag="VF589_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX8257908.1"
                     /translation="MSEDWPDIPYAPWRETCSALHLYAQIVGKYRLARTPWVNHSWHA
                     TLYVGGRGLTTGPVPEPGRTVEIEFDLIDHRLVGRTSDGRAEGFALGAGTAADFYRRF
                     LEMLDSLGASTKISRLPNEIPDALPFDADHTQRPYDADSVARFFGALTRADAVFRQFR
                     TGFIGKVSPAHLFWGSFDLAVTRFSGRPAPLHPGGIPALPDAVTREAYSHEVSSAGFW
                     PGGGGVDYPAFYSYAYPSPQGFGDARVKPAEAFFEPALGEFLLPYDAVRNAADPEAML
                     LDFLETSYAAAADLGGWDRAAVDCARGEPLKPRALG"
     gene            1128..1742
                     /gene="folE"
                     /locus_tag="VF589_09765"
     CDS             1128..1742
                     /gene="folE"
                     /locus_tag="VF589_09765"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX8257909.1"
                     /translation="MSETSPEDGDLVQASPKIPVPREVQEAVRTLIRWAGDDPDREGL
                     IDTPARVARAWREYARGYAEDPALHLSRTFDEVGGYDEIVLLKDIPFQSHCEHHLAPI
                     IGKAAIAYLPSNRVVGISKLARVLHGFARRLQVQERLTAQVADCIWEHLEPHGVAVVI
                     EASHACMTARGVNTPGVMMTTSRMMGTFRKDERSRREVLALMGY"
     gene            complement(1758..2300)
                     /locus_tag="VF589_09770"
     CDS             complement(1758..2300)
                     /locus_tag="VF589_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8257910.1"
                     /translation="MGAPKTPTPQIAVSARSVVVNPWWNVPRSIAGSIRPGAGKGYVV
                     LGGAIRQRPGPGNALGKVKIDMPNPHSINHHDTPTKPLIAESSRAFSHGCIRVKDIDR
                     LAAELLELDRGGAGEVRQALEGSATRTVALTRERPVYLVYFTADAGPGGEIVSYEDPY
                     GRDAKLLAGLNRPIQLASAR"
     gene            complement(2783..2953)
                     /locus_tag="VF589_09775"
     CDS             complement(2783..2953)
                     /locus_tag="VF589_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257911.1"
                     /translation="MRFYAGKVAAFAASIGSSLLTMASVIAAIPAPAAATPIPAPAQA
                     APLWSAQALADL"
     gene            3238..4542
                     /locus_tag="VF589_09780"
     CDS             3238..4542
                     /locus_tag="VF589_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8257912.1"
                     /translation="MTATTSSEPTTPLRRVVMASMIGTTIEWYDFFLYNSAAALVFNR
                     LFFPQYDPLTGTLLAFATYALGFVARPLGGIVFGHFGDRIGRKRLLMLSLILMGVATV
                     LIGMLPTYAQIGIAAPAALIALRLMQGFAVGGEWGGAVLMAAEHGDAARRGFWASWPQ
                     AGVAAGFLLSAGMLAAISGMLSEADFLAWGWRVPFLASFLLVVVGWYIRNRVAESRLF
                     ETALEEAEAPPRMPALEVFRERPKALLLGAGLRVGENISYYILTAFSLTFLVEISEES
                     RALALNALLAGAAVQFFAIPSFAALSDRIGRRLVYAFGAFGLAAWSFALFPLLASGDN
                     LSIVAALVVGLVLHGAMYGPQAAFITELFPTRIRYSGVSIAYQLTSIVAGSLAPIIAL
                     TLYRHYDSAIPVALYVAVTCTVSGVSALLARETRGVELADIR"
     gene            4616..>5521
                     /locus_tag="VF589_09785"
     CDS             4616..>5521
                     /locus_tag="VF589_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX8257913.1"
                     /translation="MNFLSVAFDDMNAYAFLKTLYGGALHTPNIDRVMAMGTTFENGF
                     SQVAVCNASRTAVLTGLDPGVSGVHMNYDRWAEVVDPGATLPALLRDAGYNTSLIGKV
                     FHDETTSDLVLDAISDFHFRAPESSYESSTVLTTRPLPGPVEEQGDYINVSQAIDLLN
                     GAGTDPFAMFLGIAKPHLGWVVPQEYFDLYPLDEIKLPFNVEGDLSDVPAFMKALVFD
                     ETHQQVVDANAWKTALQGYFASISFADAMLGRVLDTLEANGQLGETAILLWTDHGYHL
                     GDKDNWHKFTLWEEAARAPFVLALPG"
BASE COUNT          820 a         1812 c         1927 g          962 t
ORIGIN      
        1 ccggcctaac ccggttggcg gtcggccccg tagctcagca ggatagagca tcagattcct
       61 aatctgaggg ccacaggttc gaatcctgtc ggggtcacca ttttttgagg cggggagcgg
      121 cgcatgagcg aagattggcc cgatatcccc tacgccccgt ggcgcgagac ctgtagcgcg
      181 cttcacctct acgcccagat cgtcggcaaa taccggctcg cgcggacgcc gtgggtcaac
      241 catagctggc acgcgacgct ctatgtcggc ggccgggggc tcacgacggg gccggtgccg
      301 gagccggggc gcacggtgga gatcgagttc gacctcatcg accaccgtct ggttggacgg
      361 accagcgacg ggcgtgctga gggcttcgcg ctgggggcgg ggacggccgc cgatttctac
      421 cggcgcttcc tggagatgct ggatagcctc ggcgcgagca cgaagatcag ccggctaccg
      481 aacgaaatac cggacgcgct gccgttcgac gcggaccaca cgcagcggcc ctatgacgcg
      541 gactcggtcg cccgcttctt cggcgcgctc acccgggccg atgcggtgtt ccggcagttc
      601 cggaccggct tcatcggcaa ggtcagcccg gcccatttgt tctggggcag cttcgacctt
      661 gccgtcacac gcttttcggg ccggccggcg ccgctccatc ccggcgggat cccggcgctg
      721 cccgacgcgg tgactcgcga ggcctatagc catgaagtct cgtccgccgg cttttggccg
      781 ggcggcggcg gcgtcgatta tccggctttc tattcctacg cctatccctc gccccaaggc
      841 ttcggggacg cgcgggtgaa gccggccgag gctttcttcg aacccgcgct cggcgagttc
      901 ctgctgcctt atgacgcggt gcggaatgcg gcggacccgg aagccatgct gctggacttt
      961 ctcgagacca gctacgcggc cgccgccgac ctcggcggct gggaccgggc cgcggtcgac
     1021 tgcgcgcgcg gcgaaccgct gaagccgcgc gcgctcggct gacgggagtt cgcaaagccg
     1081 ggcggcccgt gtatcgggtg gcgcccggcc aacaacgaga gaccgaagtg agcgagacga
     1141 gccccgaaga cggcgacctg gtccaggcga gccccaagat accggtgccg cgcgaggtgc
     1201 aggaggcggt ccgcaccctc attcgctggg cgggcgacga tcccgaccgc gagggcctca
     1261 tcgacacgcc ggcgcgggtg gcgcgcgcgt ggcgggaata tgcccgcggc tatgccgagg
     1321 accccgccct ccatctcagc cgcaccttcg acgaggtcgg cggctatgac gagatcgtgc
     1381 ttctgaagga cattccgttc cagtcgcatt gcgagcatca tctggcgccg atcatcggca
     1441 aggcggcgat cgcctacctt cccagcaacc gcgtcgtcgg catctccaag ctcgcccgcg
     1501 tcctccacgg tttcgcgcgc cggctgcaag tgcaggagcg gctgacggcg caggtcgccg
     1561 actgcatctg ggagcatctc gagccgcacg gcgtggcggt ggtgatcgag gcgagccacg
     1621 cgtgcatgac cgcgcgcggc gtcaacacgc cgggcgtgat gatgaccacc agccggatga
     1681 tgggcacctt ccgcaaggac gagcgcagcc gccgcgaagt gctcgcgctg atgggttatt
     1741 aggcggcgcc ggaccaccta ccgcgcgctg gcgagctgga tcgggcggtt gaggccggcg
     1801 aggagcttgg cgtcgcggcc atagggatcc tcgtagctga cgatctcgcc gcctggcccg
     1861 gcgtcggcgg tgaaatagac cagatagacc ggccgctcgc gggtcagcgc cacggtgcgg
     1921 gtggccgatc cctccagcgc ctggcggact tcgccggcac cgccgcggtc gagctcgagc
     1981 agctcggcgg cgaggcggtc gatatccttc acccggatgc agccgtggct gaacgcgcgc
     2041 gacgattcgg cgatcaaggg cttggtcggc gtgtcgtggt ggttgatgct gtgcgggttc
     2101 ggcatgtcga tcttgacctt gccgagcgca ttgcccgggc cgggccgctg ccggatggcg
     2161 cctcccaaga cgacatagcc cttgccggcg ccgggacgga tgctgccggc gatgctgcgc
     2221 ggcacgttcc accacggatt gacgaccacc gagcgggccg agacggcgat ttgcggcgtc
     2281 ggcgtcttcg gggctcccac cagacggtgt aggtcgagac cggcttgccc tcgtcgatga
     2341 ccgccagcgt gtagctcggt acgttcacgt agatatggtc ggcgccgagc gcgcgcggca
     2401 tccagcgcca gcactcgaga ttggcgcgga gccggccctt cagcgccgcg tcgggcgccg
     2461 cggcgtaagc ggcgcgaagc gcggcatagc gcgggtcctg gggcagcagg gtgcgcagcc
     2521 acggccggac gcggttctgg cgcagcgcgg cctggagatt ggcggagagc tggttgagat
     2581 cgacgtcgcg ttcgatgtgc cagccgaagc ggctcggatc ctcgatccgg ccgcgggcat
     2641 agtcggtggc gagcgccagc gcgagcgagg tggcgatccg gtcggtcgcg gcgctgtcgc
     2701 cgccggccgc gacggtggcg aggccggcga ggtcgtaggc ggaaggatcg agcccttcga
     2761 gctcggccgc cttgacctcg gcttaaagat cggcgagcgc ctgcgccgac cagagcggcg
     2821 ccgcctgggc gggcgcgggg atcggcgtcg ccgcggcggg cgcggggatg gcggcgatca
     2881 ccgacgccat ggtcagcaag ctcgaaccga tcgatgcggc gaaggccgcg actttccctg
     2941 cgtagaaccg catgatgaat atccccatta tgttttttcg aacggctgcc gaacaatccg
     3001 cgtgcagcga tgatcgcgcc ctaacggggg tccgcgccga tacacgggat atgagatgga
     3061 ggcgggagcg gaggcgacca gatcatcgca ttcgttccgg gctcccggtt cgtcgctcgc
     3121 cgcggcagct tggtccatga tggcggcttg gtcgcggcgc aggcgagttt gagccgccgc
     3181 ttgccttgtg caggcgggcc ggtaggttcg gtgaaggccg agcgggggat cgatcaggtg
     3241 acagccacaa catcaagcga gccgacgacg ccgctccgcc gggtggtaat ggccagcatg
     3301 atcggcacca cgatcgaatg gtacgatttt ttcctgtaca attcggccgc ggctttggtc
     3361 ttcaaccgct tgttcttccc gcaatatgac ccgctgaccg gaaccctgct cgcctttgct
     3421 acctacgcgc tcggctttgt ggcgcggccg cttggcggca tcgtcttcgg ccatttcggc
     3481 gaccggatcg ggcgcaagcg cctgctgatg ctgagcctga tcctgatggg cgtggcgacg
     3541 gtgctgatcg ggatgcttcc gacctatgcc cagatcggca tcgccgcgcc ggcggctttg
     3601 atcgccttga ggctgatgca gggctttgcg gtcggcggcg aatggggcgg cgcggtgctg
     3661 atggcggccg agcatggcga cgctgcccgc cgcggcttct gggcgagctg gccgcaagcg
     3721 ggcgtggcgg ccggcttcct gctctccgcc ggcatgctcg cggcgatcag cggcatgcta
     3781 agcgaggcgg atttcctcgc ctgggggtgg cgggtgccgt tcctggcttc gtttttgctg
     3841 gtcgtcgtgg gctggtacat ccgcaaccgg gtggccgaaa gccgcctgtt cgaaaccgcc
     3901 ctcgaagagg ccgaagcgcc gccgcgcatg ccggcgctcg aagtgttccg cgagcggccc
     3961 aaagccctct tgctgggggc ggggctgcgg gtcggcgaga atatctctta ctacatcctc
     4021 accgccttct cgctgacctt cctggtggag atatcggagg agagccgcgc gctggcgctc
     4081 aacgccctgc tggcaggggc ggcggtgcag tttttcgcga ttccctcctt cgcagcgctt
     4141 tcggaccgga tcgggcggcg cctcgtctac gccttcggcg ccttcggcct cgccgcctgg
     4201 agcttcgcct tgttcccgct gctggcgagc ggcgacaatc tgtcgatcgt cgcggcactg
     4261 gtcgtgggcc tggtgctcca cggcgccatg tacggcccgc aggccgcctt catcaccgag
     4321 ctgttcccga cccggatccg atattcgggc gtgtcgatcg cctaccaatt gacctcgatc
     4381 gtcgccggct cgctcgcgcc gatcatcgcc ctgacgctgt accgccatta tgattcggcg
     4441 atcccggtcg ccctctatgt cgccgtcact tgcacggtga gcggcgtcag cgcattgctc
     4501 gcgcgcgaga cgcggggggt ggagctggcg gatatccgtt gaggggccgg cgatggacgc
     4561 aagcccctgt tcatgctaat aagctattga ccggcaaacg ctttttgggg ggtccatgaa
     4621 ttttctgtcg gtcgcgttcg acgacatgaa cgcctatgcg ttcctgaaga cgctttatgg
     4681 cggcgccctc catacgccca atatcgatcg cgtgatggcg atgggcacga cgttcgagaa
     4741 cggcttctcg caagtcgcgg tctgcaacgc ctcgcgcacg gcggtgctga ccggcctcga
     4801 tcccggcgtg tccggcgtcc acatgaatta cgaccgctgg gccgaggtgg tcgatccggg
     4861 cgcgacgctg ccggcgctgc tccgggatgc gggctacaat accagcctga tcggcaaggt
     4921 cttccacgac gagaccacct ccgacctggt gctcgacgcg atcagcgact tccactttcg
     4981 cgcgcccgag agcagctatg aaagctcgac cgtgctgacg acccggccgc tgccgggacc
     5041 cgtcgaggag cagggcgact atatcaatgt cagccaggcg atcgacctgc tgaacggggc
     5101 aggcaccgat cccttcgcca tgttcctcgg catcgccaag ccgcatctcg gctgggtcgt
     5161 tccgcaggaa tatttcgatc tctatcccct cgacgagatc aagctgccgt tcaacgtcga
     5221 gggcgatctg tcggacgtgc cggctttcat gaaggcgctg gtgttcgacg agacccatca
     5281 gcaggtggtc gacgccaatg cctggaagac ggcgctgcag ggctatttcg ccagcatctc
     5341 cttcgccgac gcgatgctcg ggcgggtgct cgacacgctc gaggccaacg ggcagcttgg
     5401 cgagaccgcg atcctgctct ggaccgacca cggctatcat ctcggcgaca aggacaattg
     5461 gcacaaattc accttgtggg aagaggccgc ccgcgcgccg ttcgtgctgg cgctgccggg
     5521 c
//
LOCUS       DASVRK010000246         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_111672, whole genome shotgun sequence.
ACCESSION   DASVRK010000246 DASVRK010000000
VERSION     DASVRK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111672"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2654
                     /locus_tag="VF589_09790"
     CDS             <1..2654
                     /locus_tag="VF589_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015474 - autotransporter activity
                     [Evidence IEA];
                     GO_process: GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX8257914.1"
                     /translation="VSTAGYAANGITSYSVLGTSTITIDKVTTAGERSGGVRARGGTG
                     ATVIANVVETHGYGIDAGAVTGTVLVKVNEVTTTGSAYAEGIQLVSIFGSGRIESGTI
                     STIGKNSSGIGLLLGGQPGYAGDAVVISGSIATQGENSDGIRGEAYVGSLTIESGSIT
                     TAGYEADGIDALAGGNVTIASEAIATSGIISDGIRAVSQLSPFGGEPGSVSIASESVS
                     VSGDSSRGIVGLAEGDVTIVSGAVATSGGTAIVESLELVCDDETGYCNYETVRRPGPR
                     PVGILAKSNRGSVTITSGSVTTRGRGAHGIHAITEAGAITIASGDISVLGGNASAIRA
                     ESYSGDIAVTIDGDVSAARAPAVEATTGGGVRVTVANGGLLRSSGDQFALVANAASAD
                     VQVAAGGTLAGSVSLTGGADTLTVAGLFDARGESRFGDGADLLTNEGTLAVRSGTVRF
                     AGLERFRNAGLITLADGSADDVLDLTGTAFEGAAGSAVRFDVVTGAGGAAAADQLILG
                     DVSGVTEVQVALQGPATLGTRADLITVSGDSPDGAFVLDPAFGDAGFLRYRLVGVGTG
                     TIALATAPDREIFEAGRIAPAAANLWRHGAEAWAAQRLAARDSGEAGRGVWGQAFTGG
                     QTVEDLGYGTSFDGETYGETLSTKEKYSGLQFGGDLIGGPVRLGVTAGYGDADLRLRE
                     SGNEIKAKGFNVGVYGGWSNRLLFVDLLGKIDFADIEFDARSAGARRDFDATIYGLRA
                     DAGLRLGGETFFAEPMVSLAWTNASIDDFEVDQGEFRLGGGSGLLGQAGLRAGGRWAL
                     GGGYALSPSGGLFAVKELAGGNGLGFHSGTASLAIEGDRPGLYARAEGGLTLSSGSGW
                     DFSLRGQGDFGGDVSGAAVRAGFNWRW"
BASE COUNT          401 a         1028 c         1002 g          397 t
ORIGIN      
        1 atgtttcgac cgccggctat gccgccaacg gcatcaccag ctattccgtc ctcggcacca
       61 gcacgatcac catcgacaag gtgacgaccg cgggcgagcg ctccggcggc gtccgcgcgc
      121 gcggcggcac cggcgcgacc gtgatcgcca acgtcgtcga gacccacggt tacggcatcg
      181 atgcgggcgc ggtgaccggt acggtgctgg tcaaggtcaa cgaggtgacc accaccggca
      241 gcgcctatgc cgagggcatc cagctggtca gcatcttcgg ctcgggccgg atcgagagcg
      301 ggacgatctc gacgatcggc aagaattcca gcggcatcgg gctgctgctc ggcggccagc
      361 cgggctatgc gggcgacgcg gtggtgatca gcggcagcat cgccacccaa ggcgagaata
      421 gcgacggcat ccgcggcgaa gcctatgtcg gctcgctgac catcgagagc ggatcgatca
      481 cgaccgccgg ctatgaagcg gacggcatcg acgcgctggc cggcggcaac gtcaccatcg
      541 ccagcgaggc gatcgccacc tcgggcatca tctccgacgg catccgcgcg gtcagccagc
      601 tgtcgccgtt cggcggcgag cccggctcgg tcagcatcgc cagcgagagc gtgtcggtga
      661 gcggcgattc ctcgcgcggc atcgtcggcc tggccgaggg cgacgtcacc atcgtcagcg
      721 gcgcggtcgc caccagcggc ggcaccgcca ttgtggagag cctggagctc gtctgcgacg
      781 acgagaccgg ctattgcaat tacgagacgg tgcgccggcc ggggcctcgg ccggtgggca
      841 tcctcgcgaa atccaaccgc ggctcagtca ccatcaccag cggctcggtg accactcgcg
      901 gccgcggggc gcacggcatc cacgcgatca ccgaagcggg ggccatcacc atcgccagcg
      961 gcgacatcag cgtgctcggc ggcaacgcca gcgcgatccg cgccgaaagc tattcgggcg
     1021 acatcgctgt caccatcgac ggcgacgtgt cggccgcgcg cgctccggcg gtcgaggcga
     1081 ccaccggcgg cggcgtgcgc gtgacggtgg cgaatggcgg cctgctgcgc agctccggcg
     1141 accagttcgc tttggtggcg aacgccgctt cggccgatgt ccaagtggcc gccggcggca
     1201 ccctcgcagg ttcggtgagc ctcaccggcg gtgccgacac gctcaccgtc gccggcctgt
     1261 tcgacgcgcg cggcgagagc cgcttcggcg acggcgcgga tctgctgacc aacgaaggca
     1321 ccctcgccgt ccggtccggc acggtgcggt tcgccggcct cgagcgattc cgcaacgccg
     1381 gcctcatcac cctcgccgac ggaagcgccg acgacgtgct cgacctcacc ggcaccgcct
     1441 tcgaaggcgc ggccggcagc gcggtccgct tcgacgtcgt caccggcgcc ggcggcgctg
     1501 cggcggccga ccaactgatc ctcggcgacg tcagcggcgt caccgaggtg caggtcgcgc
     1561 tgcagggccc ggcgacgctc ggcacccgcg ccgaccttat caccgtcagc ggcgacagcc
     1621 cggacggcgc cttcgtgctc gaccccgctt tcggcgatgc cggcttcctg cgctaccgcc
     1681 tggtcggcgt cggcaccggc accatcgcgc tcgccaccgc gcccgaccgc gagattttcg
     1741 aagccggccg catcgccccc gccgccgcca atctgtggcg gcacggcgcc gaggcctggg
     1801 cggcgcagcg gctcgccgcg cgcgattccg gcgaggccgg gcgcggtgtc tggggccagg
     1861 ccttcaccgg gggccagacc gtcgaggacc ttggctacgg cacgagcttc gatggcgaga
     1921 cctatggcga gacgctgtcc accaaggaga aatatagcgg cctccagttc ggcggcgacc
     1981 tgatcggcgg cccggtgcgg ctcggcgtca ccgccggcta tggcgacgcc gacctgcggc
     2041 tccgcgagtc cggcaacgag atcaaggcga agggcttcaa cgtcggcgtc tatggcggct
     2101 ggagcaaccg gctgttgttc gtcgacctgc tgggcaagat cgacttcgcc gacatcgaat
     2161 tcgacgcccg ctcggccggc gcccgccgcg acttcgatgc gactatctac ggcttgcggg
     2221 ccgatgccgg cctgcggctg ggcggcgaga ctttcttcgc cgagccgatg gtgtcgctcg
     2281 cctggaccaa cgcgtcgatc gacgacttcg aagtggatca gggcgagttc cggctcggcg
     2341 gcggcagcgg cctgctcggc caggccggcc tccgcgccgg ggggcgctgg gcgctgggcg
     2401 gcggctatgc gctcagcccc tcgggcggcc tcttcgccgt aaaggaactg gcgggcggca
     2461 acggcctcgg cttccattcg ggcaccgcca gcctcgccat cgagggggac cggcccggcc
     2521 tctacgcccg cgccgaaggc ggcctcaccc tgagcagcgg cagcggctgg gacttctcgc
     2581 tgcgcggcca gggcgatttc ggcggcgacg tgtcgggggc ggcggttcgc gcgggcttca
     2641 actggcgttg gtagccgcgc catgagccag cccgaagcgg gcgcccccgc cgccgcgccc
     2701 gagacccgga tcgagctgtc gcgcggcttt gccggctggc tgcggcagat gggctgcagc
     2761 ctcgccttta cgtcttatca aaccggcaaa ctcttcctgg tcggcctgct ccgcgacggc
     2821 cgcgtctc
//
LOCUS       DASVRK010000247        12503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_359636, whole genome shotgun sequence.
ACCESSION   DASVRK010000247 DASVRK010000000
VERSION     DASVRK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12503
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359636"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VF589_09795"
     CDS             complement(<1..415)
                     /locus_tag="VF589_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8257915.1"
                     /translation="MALTAIAVNCSLKRSSGDDSSTDKMIGLIVSELKKKGVAFLETI
                     RIADHEVKPGVTSDEGEGDDWPDLRRRILAADILIFGTPIWMGQASSIAKRVVERMDA
                     FLSETDAAGRMPSYGRVAVLAVVGNEDGAHQVSAQL"
     gene            complement(538..4707)
                     /locus_tag="VF589_09800"
     CDS             complement(538..4707)
                     /locus_tag="VF589_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257916.1"
                     /translation="MLYGKTAGLGTLDLAALDASDGFFLQGTEDDRISGEQVSAAGDL
                     NGDGFADVVVGAPRYSYPTYYGGMVYVVYGGDDRSGTVQLAELSGQEGFTLAGTGYDS
                     WTGSTLAPVGDFNGDAIGDLIVGNTSGEAYLVFGKRGGLESSSIVSADGFTIQGAQFS
                     GYRIDGAGDFNGDGLGDLIIGLPGDDTAASNAGRAVILLGKRRGFQDVDLSALDAEDG
                     YFIQGDGAADQVGWTVSSVGDFNGDALDDIVTGTVEGHLYIVFGTNGDTSTIGLAGLD
                     ASAGLLITGTHLDSWTKLAAAGDVNGDGFDDVMITARSPQSQPGGQIFIVFGKANGSG
                     PIDLDNLVESDGFAIQAGGNGEYVGKDMASAGDVNGDGCSDLLISTRSDADTKYLGTT
                     YVIYGLTPDEAVTRTGTAAGQTLAGGAGDDRLDGRAGDDSLYGNGGIDVIIGGTGHDR
                     LNGGLGDDRLTGGNGNDIYVLDSKLDRTTELAGGGVDTVRSNFNFTLRAEFEALELLG
                     SGNLSGIGNGAANRIVGNRGDNFLDGRGGADRMSGGAGDDQFIVDVAEDVVTEKENGG
                     TDTIKSAASFVLGANVENLILTGSAAIDAAGNAAANRLSGNSAANVIDGGEGADRMTG
                     GAGNDRYVVDDAGDVIVEAANGGTDAVESSVSHRIAATVENLTLTGSSDIDGDGNAAA
                     NSIAGNAGANRLNGREGADTLAGGAGDDTYIVDHAGDSLVEALAAGTDRVNSTVSHTL
                     GANFEILTLAGTAAIAGTGNALANAITGNAAANALRGDGGNDTLDGKAGGDSMHGGAG
                     SDLYVVDHGRDAVTELAGAGIDTVESSVTHTLDANVERLVLTGAAAIGGVGNALANVL
                     TGNAAANILNGGGGNDLMTGGAGDDTYVVDRTSDRVVELSAGGVDLVRSSASYVLADH
                     VENLALTGGASASATGNALANQLTGNSGNNLLDGLGGSDSMAGGNGNDTYIVGGAGDI
                     VIEAASGGVDRVMTSLSHSLSEGVEQLTLTGTANVDASGNSLANLLTGNGGRNRLDGR
                     AGADRMVGAGGNDVYVVDHAGDQAVESAAGGVDLVESAASYTLGRELEQLTLTGNGSI
                     DATGNAIANLLTGNNAANRMDGGVGVDTMSGGAGDDSYVVDNVGDRIVELQGRGFDTI
                     ESSVSYSVYGYVEQLTLVGTAAIDAKGNSLSNALTGNSADNLLDGGAGRDRLAGGDGN
                     DTYIVDHADDVIVEAESQGTADLVKSIVSYQLSDHVEELVLGGSGAINGTGNGSDNRI
                     TGNAAANQIGGGGGNDSLLGGAGADTFRFDTALDSLANVDAILDFRVVDDTIRLNQGV
                     FGTLPLGTLRASAFRAGDAAADADDRIVYDADSGRIFYDAEGSGVGAALLFATVTAGT
                     ALTNADFVIQG"
     gene            5162..5503
                     /locus_tag="VF589_09805"
     CDS             5162..5503
                     /locus_tag="VF589_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257917.1"
                     /translation="MDQIYWLGRTRAAAAMARKAAGAEARLAHLELAGRYSIKAATAA
                     AAARKRAPAQAEQPRLAAPSADPLRYDQLEQGARYLAGKAGGDSERRRHLGMANRYAQ
                     MAQDEIDHEGR"
     gene            5505..5729
                     /locus_tag="VF589_09810"
     CDS             5505..5729
                     /locus_tag="VF589_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257918.1"
                     /translation="MTIAPEPRQMESGGPSERPGTVERAFQLARSGDCRSLGEIVTRL
                     KREQHDGVDAHFAGPSIKRELRRICEANRD"
     gene            5793..6416
                     /locus_tag="VF589_09815"
     CDS             5793..6416
                     /locus_tag="VF589_09815"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX8257919.1"
                     /translation="MKAALAALALALALGGCREALSPTAEYRPEEAGTVDHALCLLGF
                     TAVPLRKLVTGHHLVQAKLNGRDGIFLLDTGANASVLDAGAAADFGVGGGAAVPGAAV
                     GLGGAMKARQSAIESLAIGPVPIRRDRIMTADLGQVVKVLGPLSGGAIHGIIGQDVME
                     EHRAVIDVARPILYLTPEDAQPTPVPAERCRAEAGEGEPAANRSATS"
     gene            6494..7255
                     /locus_tag="VF589_09820"
     CDS             6494..7255
                     /locus_tag="VF589_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8257920.1"
                     /translation="MVSPWITKMEQFTCFSGDERARLETLSAARQEDYAARQDIVREG
                     DTPSEIHIVLSGLATRYAILPDGSRQIMAFLIPGDMCDAEVFVLSEMDHGIGALAPTR
                     CAVVPARTMKGLLREVSPLAEALWWSTMTDSAVLRQRIVDHGRRDARERIAHLFYEML
                     IRYRMVGLAPDNRFEFPVTQDEIADATGLTPVHVNRMVQQLRDGGLIEMKGKVLRVTD
                     PEGLKQVARFNANYLHLADGPRGEEGSSRALPQPS"
     gene            complement(7273..7521)
                     /locus_tag="VF589_09825"
     CDS             complement(7273..7521)
                     /locus_tag="VF589_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257921.1"
                     /translation="MPRFHLHQRGGVRDFTDEDGQDLPGLTAAHQHAIDGIRSILADE
                     ILHGRLPLGERIDIEDEAGRVLLSVDFAESFRLEPSSA"
     gene            complement(7535..8287)
                     /locus_tag="VF589_09830"
     CDS             complement(7535..8287)
                     /locus_tag="VF589_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8257922.1"
                     /translation="MTDSSPLQPMLDKLERHVRFQEEDRAAMLALPHTLRTVEPSKYI
                     VREGDRAENACLLRSGFAFRHKTVGDGGRQIVAIHMKGDLVDLQNSLLRTADHSVQAL
                     TEAEIALIPRRAIVELCARNAIVAQAMWLDTLVDGSVFREWIANVGRRDARSRTAHVL
                     CEFAIRQESAGLGERGEYELPMTQEQLADVLGLTPVHVNRTLKSLEADGLISRNKRSV
                     TIGSWENLRAAGDFSPAYLHLELIEDGSAARS"
     gene            8532..8816
                     /locus_tag="VF589_09835"
     CDS             8532..8816
                     /locus_tag="VF589_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257923.1"
                     /translation="MPRFFFNVRDEAGFAEDEEGLNLGRAEEARDIAIDGARSILSDQ
                     ARQGRVNLRGRVEVADAGGVPLFSVDFGEAVEVVAGPLDIPDPAAAGRDP"
     gene            8813..9535
                     /locus_tag="VF589_09840"
     CDS             8813..9535
                     /locus_tag="VF589_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8257924.1"
                     /translation="MIGEALVKHLGLIGHLDKADEAALLGIKGEIRDVARDEDLLREG
                     ERPGEAVVVLAGFLQRYTLTAQGERQVHSFYMATDTPCLETLHIDYMDNNLGAVVPSR
                     VGAVAHAELFRVMDLRPNLLALIWRETLIQGAIFREWLMRNSRMLAHVQLAHFFCEML
                     TRARAAGIADGDSCELPLTQQDLADVLGMTAVHVNRTLMMLRSAGLIEFRAGRLIVRN
                     WDELVEIAEFDPHYLHLRRPAQ"
     gene            complement(9529..9966)
                     /locus_tag="VF589_09845"
     CDS             complement(9529..9966)
                     /locus_tag="VF589_09845"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEX8257925.1"
                     /translation="MSRASLFLAAALLAALPAAADACSVSTAGIAFGAYNPRSATARD
                     SAGTINVDCPRNSDVVISVSAGRSGTFTQRRMTNGTSNLNYNLFTNAGRSIVLGDGSG
                     GTATFSIAKLRGAASRTFYGRIPGSQNVSAGAYAETLIVTVSY"
     gene            complement(9953..12289)
                     /locus_tag="VF589_09850"
     CDS             complement(9953..12289)
                     /locus_tag="VF589_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HEX8257926.1"
                     /translation="MARSSSPARRTKLWLRPLLLLIAPGAAQAAELASAPQPMLLEVT
                     LNGQSMGEPWLVLRDPAGGTWIAAALLGKSGLRIGGCESANFEGESYCRVDKLPAMTL
                     TASDAEQRLALQAEPGLFERQTASLAPPAAGEMSDPGTGAFLNYELLAETARGKLRAG
                     GAFEAGLYTPHGVGIATFVADVGGGATRLTRLETNWTHDDPENLTSIRIGDSVTRAGP
                     HAAPIRFAGIQYGRNFAVRPAFITMPLPSVEGSAAMPSVVDIYVNNSLRGSRTVTPGP
                     FELTDVPVRSGGGTVDVVTRDLLGREVVTSLSYYAGADMLQRGLHDFSYELGFERRGF
                     GYKSHSYGALSASTTHRFGLTDHVTIEGHVQASRSRQAASGAVTAAWPGIGLVSVSAA
                     GSRGRHGIGGALGAAFERRTEGLSLGLRSELTTARYTYAGLPAGYEIPRLTVQGFADL
                     PVKRMTVGVNVLHRDFRYKPEETLLGAFATLPLGRLGSLQLYARRTALGQSETSFGGH
                     FVLPLGGRRSASASYEHRRRGSLASATFQSDPPVGNGSGFRVAATTGAVDSARAEYRL
                     QTQYAAFGAEVAYAEGGAGVRLSAAGAVGTIAGDSFASRRLGDSFAAVEVKGHKGVRV
                     YADNQLIGRTGADGRLVLPALRAFDDNRIRIDETDLPMDVQLARNEASVRPFARSGAV
                     IRFDAKRERGVLLRVALADGSFLPAGAEVGVEESDERLVVATHGEIYVPDMAGTRRFR
                     ARIGAKSCTFEATAPDNDDPQPLIDGLICVEAPQYVAR"
     gene            complement(12255..>12503)
                     /locus_tag="VF589_09855"
     CDS             complement(12255..>12503)
                     /locus_tag="VF589_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257927.1"
                     /translation="DGSWAVEARNGGAGHVRVDAAQASAATGISRIPALDFAVLLPHG
                     SRRWTIASAPAVKDRIAFQKIVRPGNGDGPQQLARKAN"
BASE COUNT         1918 a         4146 c         4384 g         2055 t
ORIGIN      
        1 agagctgggc gctgacctga tgagcgccgt cttcgttgcc gacgacggcg aggacggcga
       61 cccggccgta gctcggcatc cggccggccg catcggtctc ggacaggaag gcatccatcc
      121 gctcgaccac ccgcttggcg atgctcgacg cctggcccat ccatatcggg gtgccgaaga
      181 tgaggatgtc ggccgcgagg atgcggcggc gcaggtccgg ccagtcgtcg ccttcgccct
      241 cgtcggacgt gacgccgggc ttgacctcat gatcggcgat gcggatggtc tccaggaacg
      301 ccacgccctt cttcttgagc tcggacacga tcaggccgat cattttgtca gtcgacgaat
      361 cgtcgccgct cgaccgtttc aggctgcaat tcacagcgat agccgtcagc gccatgtccg
      421 ctctccttca tcgagccaga cgcgttccgg gtccggaatg ttcccgaggc ggtcccggcc
      481 gcgcacgcga cgctgtagag gtaggggcag cgctttcgcg ccaccccgta cccctcgtca
      541 gccctggatc acgaagtcgg cgttggtgag cgccgtcccg gccgtgacgg tggcgaacaa
      601 tagggccgca cccacgcccg atccctcggc atcgtagaag atcctgccgc tgtccgcgtc
      661 gtagacgatg cgatcgtcgg catcggccgc agcgtcgccg gcccggaacg cgctcgcgcg
      721 cagcgttccc aggggaagcg ttccgaacac cccctggttg aggcggatgg tgtcgtcgac
      781 gaccctgaaa tcgaggatgg catcgacgtt ggcgaggcta tcgagcgcgg tatcgaagcg
      841 gaacgtgtcg gcgccggccc cgcccagcaa gctgtcattg ccgccgccgc cgccgatctg
      901 gttcgcggcc gcattgcccg tgatccgatt gtcgcttccg ttgccggtgc cgttgatggc
      961 ccccgatcct ccgaggacca gctcctcgac atggtcggaa agctggtagc tgacaatgct
     1021 cttcaccagg tcggccgtgc cttgggattc ggcctcgacg atgacgtcgt cggcatggtc
     1081 gacaatgtag gtgtcgttgc cgtcgccgcc cgccagcctg tcccggccgg ctccgccgtc
     1141 gagcagattg tcggccgagt tcccggtcag cgcgttggag aggctgttgc ccttggcgtc
     1201 gatggcggcg gttccgacca aagtcagctg ctccacatag ccatagacgg agtagcttac
     1261 cgagctctcg atcgtgtcga accctcgccc ctgaagctcg acgatccggt cgccgacatt
     1321 gtcgaccaca tagctatcgt caccggcgcc gccgctcatc gtgtcgacgc cgacgccgcc
     1381 gtccatgcgg tttgccgcat tattgccggt cagcaggttc gcgattgcgt tgccggtcgc
     1441 atcgatcgag ccgttgccgg tgagcgtcag ctgctcaagt tcgcggccca gcgtgtagct
     1501 ggcggcgctt tcgaccaggt ccacgccgcc cgcggccgat tccaccgcct ggtcgcctgc
     1561 gtggtccacc acgtagacgt cgttgccgcc cgcgccgacc atccggtccg cgcccgcgcg
     1621 cccgtccagt cggttgcggc cgccattgcc ggtgagcagg ttggcgagcg aattgccgct
     1681 ggcgtcgacg ttcgccgtcc ccgtcagggt gagttgctcg accccttcgg agagcgaatg
     1741 gctgagcgag gtcatcaccc ggtcgacgcc gccggaagcg gcctcgatca caatgtcacc
     1801 cgcaccgccg acgatgtagg tgtcgttgcc gttgccgccg gccatgctgt cgcttccgcc
     1861 cagcccgtcg agcagattgt tgccggaatt cccggtcagc tggttggcca aagcgttgcc
     1921 cgtcgcgctg gccgacgcac cgccggtcaa ggcgagattc tcgacatggt cggccagcac
     1981 gtagctggcg gagctcctga cgagatcgac gccgcccgcc gagagctcga ccacgcggtc
     2041 gctggtccgg tcgacgacgt aagtatcgtc gccggcgccg ccggtcatca gatcgttgcc
     2101 gccgccgcca ttgaggatgt tggcggccgc gttgccggtg aggacgttgg caagcgcatt
     2161 gccgaccccg ccgatcgccg cggcgccggt aagcacaagc cgctcgacat tggcgtcgag
     2221 cgtgtgggtg accgagctct cgaccgtgtc gatgcccgcg cccgcaagct cggtcacggc
     2281 gtcgcgccca tgatcgacca catagaggtc gctgccggcg ccgccgtgca tgctgtcgcc
     2341 gcctgccttg ccgtcgagcg tgtcgttgcc gccatcgccg cgaagcgcgt tggcggcggc
     2401 gttgccggtg atcgcgttgg ccagcgcatt gcccgtcccc gcgatcgccg ccgtgccggc
     2461 gagagtcagg atctcgaaat tcgcgcccag cgtgtggctc acggtcgaat tcacccggtc
     2521 ggtcccggcg gcgagagcct cgaccaggct gtcgccggca tggtcgacga tataggtgtc
     2581 gtcgccggcg ccgccggcca gggtgtcggc gccctcgcgg ccgttgagcc tgttggcacc
     2641 ggcatttccg gcgatgctgt tggctgcggc attgccatcg ccgtcgatgt ccgaacttcc
     2701 ggtcagggtc aggttctcga cagtcgccgc gatcctgtgg ctgacgctgc tttcgaccgc
     2761 gtcggtgccg ccgttcgcgg cctccacgat cacgtcgccg gcatcgtcga cgacataacg
     2821 gtcgttgccg gcgccgccgg tcatgcggtc ggcgccttct ccgccgtcga tgacgttggc
     2881 ggccgaattg cccgagagcc tgttcgccgc cgcatttccg gcggcatcga tggcggccga
     2941 tccggtgagg atgaggttct cgacattggc gccgaggacg aagctcgccg ccgactttat
     3001 ggtatcggtc ccgccattct ctttttcggt gacgacatcc tccgcgacat cgacgatgaa
     3061 ctggtcgtcg cccgcgccgc cgctcatccg gtccgcgccg ccgcggccgt cgagaaaatt
     3121 gtcgccgcga ttgccgacga tccggttggc ggcgccgttg ccgatgccgc tgagattgcc
     3181 gctgcccagc agttcgagag cttcgaactc ggccctgagc gtgaagttga agttgctgcg
     3241 gacggtatcc acgccgccgc cggccagctc ggtcgtcctg tcgagcttgc tgtcgaggac
     3301 ataaatgtcg ttgccgttgc cgccggtcag ccggtcgtcg cccaggccgc cgttgagccg
     3361 atcatggccg gtcccgccga tgatgacatc gattccgcca ttgccgtaca ggctgtcgtc
     3421 gccggcgcgg ccatcgagcc ggtcgtcgcc cgcgccgccc gcgagcgtct ggccggcggc
     3481 cgtgccggtc cgcgtcacgg cctcgtcggg cgtgaggccg tagatgacat aggtggtccc
     3541 gagatacttg gtgtccgcat cggagcgggt tgagatcaga agatcgctgc aaccgtcgcc
     3601 gttaacgtcg ccggcgctgg ccatatcctt gccgacatat tcgccgttgc ccccggcctg
     3661 gatcgcgaag ccgtcggact ctaccaggtt gtcgagatcg atcggtcccg atccattcgc
     3721 ttttccgaag acgatgaata tctggccccc cggctgggat tggggagagc gtgcggtgat
     3781 catcacatcg tcgaaaccgt cgccgttcac atctcccgcc gcggccaact tagtccagct
     3841 gtccagatgg gtacccgtga tcaggaggcc ggcagacgca tcgagccccg caaggccgat
     3901 cgtcgaggtg tcgccgttcg taccgaagac gatataaaga tggccttcga cggtgccggt
     3961 cacgatgtcg tcgagggcgt ctccattgaa gtccccgacg gacgagaccg tccatccgac
     4021 ctgatcggcc gcgccgtcac cttggatgaa atagccatct tcagcgtcca gggcggaaag
     4081 atcgacatcc tgaaagccgc gccgtttccc gagcaggatg acggccctgc ccgcgttcga
     4141 cgcggcggta tcgtcgccgg gcaagccgat gatcaggtcg cccagcccgt ctccgttgaa
     4201 gtcccctgcg ccgtcgatcc tgtagccgct gaattgggca ccttgaatcg tgaacccgtc
     4261 cgcggaaacg atcgaggagg attccaggcc gccgcgtttg ccgaacacga gataggcctc
     4321 tcccgaggtg ttgccgacga tcagatcgcc gatcgcgtcg ccgttgaagt cgcccaccgg
     4381 tgcaagcgtg ctcccggtcc agctgtcata gccggtgccg gcgagggtga acccttcctg
     4441 cccggaaagc tcggccagtt ggaccgttcc ggatcgatcg tcgccgccat agacgacgta
     4501 gaccatgccc ccataataag tggggtagct gtagcggggg gctccaacga cgacatcggc
     4561 aaagccgtcg ccgttcaggt ccccggccgc ggaaacctgt tcgccggaaa tcctgtcgtc
     4621 ttcggtgccc tggaggaaaa aaccgtcgct tgcatcgaga gcggccagat ccagcgtccc
     4681 gagaccggcc gttttgccgt acagcacgta tgcgcccccg ggcgcgcgcc cgttcccggt
     4741 gaacggcgtc gaaatcagaa aatcgtcgat gccgtcggcg ttgaaatcac ctgccgacga
     4801 gacgctcatc ccaagctgcg catagggttc cgtaccacgc aataggaagc cacgaacgcc
     4861 gagattcccc agatttatat tggtcacgaa tcataccccc cccacacaat atcgaacgcg
     4921 tatcagatgg ttgctgcacc tgcgagatgg cagtcggcgg accgccggcc tgacctggag
     4981 ggcggccatg gctcaagcag gagcggcgcc aagcctctgc cgctagggca aggcgggtgc
     5041 ggccgtcatt cgctatttgc agggcaatct gctatacggg cccgtcacct gatttttcag
     5101 caggcatcct gtgacttgag agacgcatgc gctctcgctt gcccagtaag aggtgatcga
     5161 catggaccag atttattggc tcgggcgcac gcgcgccgcc gccgccatgg cccgcaaggc
     5221 ggccggggcc gaggctcggc tcgcccatct cgaactggcc ggccgctaca gcatcaaggc
     5281 cgcgaccgcc gccgccgcgg cgcgcaagcg ggccccggcg caggccgaac agccgcggct
     5341 cgcggcgcca tcggccgacc cgcttcgcta cgaccagctc gagcagggcg cgcgctatct
     5401 ggccggcaag gcggggggcg acagcgagcg ccgccgccac ctcggcatgg ccaaccgcta
     5461 tgcgcaaatg gcgcaggatg aaatcgacca tgagggacgc tgatatgaca attgcgcccg
     5521 agcctcggca gatggagtcc ggcggaccgt cggagcggcc cgggacggtg gagcgcgcct
     5581 tccagctcgc ccgctcgggc gattgccgca gcctcggcga gatcgtcacg cggctgaagc
     5641 gcgagcagca tgacggcgtc gatgcgcatt tcgccggccc gtcgatcaag cgcgagctgc
     5701 gccgcatctg cgaagcgaac cgcgactgaa gttgcgcgac tgagctttgc ggcccggcgg
     5761 gaaccatcaa gccgcgccgc cattgcccgg cgatgaaagc cgccctcgcc gcgctcgcgc
     5821 tcgcattggc cctcggcggc tgccgcgagg cgctgagccc gaccgccgaa taccggccgg
     5881 aggaggcggg gacggtcgac catgccttgt gcctgctcgg cttcaccgcg gtgccgctgc
     5941 gcaagctcgt caccggccat catctcgtcc aggcgaagct taacgggcgc gacggcatct
     6001 tcctgctcga taccggcgcc aatgcgtcgg tgctcgacgc cggcgcggcg gcggatttcg
     6061 gggtcggcgg cggagccgcg gtgccgggcg cggcggtcgg gctcggcggg gcgatgaagg
     6121 cgcggcagtc ggcgatcgaa agcctggcga tcgggccggt gccgatccgc cgcgaccgga
     6181 tcatgaccgc cgatctcggc caggtggtga aggtgctggg gccgctctcc ggcggcgcga
     6241 tccacggcat tatcggccag gacgtgatgg aggagcatcg cgccgtaatc gacgtggcgc
     6301 ggccgatcct gtatttgacg ccggaggatg cgcagcccac gccggtgccg gcggagcggt
     6361 gccgggcgga ggccggcgag ggcgaacccg cggccaaccg gtcggcgacc tcgtaatcgc
     6421 cgcaaggcgc cgggcgcggg cttctcattg actctgccgg actttaaccc atgttaaggg
     6481 ggaaggagtc gagatggtta gcccctggat cacgaagatg gagcagttca cctgcttcag
     6541 cggggacgag cgcgcgcggc tcgagacgct gtcggcggcg cggcaggagg attatgccgc
     6601 ccggcaggac atcgtccgcg agggcgacac gccgtccgaa atccacatcg tcctgtcggg
     6661 gctcgccacc cgctacgcga tcctgcccga cggttcgcgc cagatcatgg cgttcctgat
     6721 ccccggcgac atgtgcgacg ccgaggtgtt cgtgctgagc gagatggacc acggcatcgg
     6781 cgcgctggcg ccgacgcgct gcgcggtcgt ccccgcccgc accatgaagg ggctgctgcg
     6841 cgaagtctcg ccgctcgccg aagccttgtg gtggagcacc atgaccgact cggcggtgct
     6901 gcgtcagcgg atcgtggacc atggccggcg cgacgcgcgc gagcggatcg cccacctctt
     6961 ctacgaaatg ctgatccgct accgcatggt tgggctcgcc ccggataatc gctttgaatt
     7021 cccggtgacg caggacgaga tcgccgatgc gaccggtctc accccggtcc acgtcaaccg
     7081 catggtccag caattgcgcg acggcgggct catcgaaatg aagggcaagg tcctgcgcgt
     7141 gaccgacccg gagggcctga agcaggtcgc gcggttcaac gccaattacc tccacctcgc
     7201 cgacggtccg cgcggggagg agggaagctc ccgggcgctg ccccagccct cctgacacca
     7261 actgacgcgg cgtcaggccg aggacggctc cagccggaac gattcggcga aatcgacgct
     7321 gagcagcacc cggccggcct catcctcgat atcgatgcgc tcgcccagcg gcagccggcc
     7381 gtggaggatc tcgtcggcga ggatcgagcg gatgccgtcg atcgcgtgct ggtgcgcggc
     7441 ggtgagacca gggagatcct gcccgtcctc gtcggtaaaa tcgcgaacgc cgccgcgctg
     7501 gtggagatgg aaccggggca tgagcggccg atcttcagga tcgcgccgcc gagccgtctt
     7561 cgatcagctc gaggtgcaga taggcggggc tgaaatcgcc cgccgcgcgc aaattctccc
     7621 agctgccgat cgtcaccgag cgcttgttgc gcgagatgag gccatccgcc tcgagcgact
     7681 tcaaggtccg gttgacgtgg accggggtga gcccgagcac gtcggcaagc tgctcctgcg
     7741 tcatcggcag ctcatattcg ccgcgctcgc cgagccccgc cgattcctgc cggatcgcga
     7801 actcgcacag cacatgggcg gtccgggagc gggcgtcgcg gcggccgaca ttggcgatcc
     7861 attcgcggaa caccgagccg tcgaccaagg tatcgagcca catcgcctgg gcgacgattg
     7921 cattgcgcgc gcagagctcg actatagcgc ggcgcggaat aagggcgatc tccgcttcgg
     7981 tgagcgcctg gacgctgtgg tcggcggtcc tcagcagcga attctggagg tcgaccagat
     8041 cgcccttcat gtggatggcg acgatctggc ggccgccgtc gccgaccgtc ttgtggcgga
     8101 aggcgaagcc ggagcgcagc aggcaggcat tttccgcgcg gtcgccctcg cggacgatat
     8161 atttgctggg ttcgacggtc cgaagcgtgt ggggaagggc gagcatggcc gcacgatcct
     8221 cctcctggaa acggacgtga cgttccagct tgtcgagcat cggctggagc ggggaggagt
     8281 cggtcatggg ggctctccgt cgggcgagag cgcttcgtct cacagtcgcc ggcaggcctg
     8341 aatcaggctg gcgatgaaaa cgctatagac ctgatcagga agctccgtcc taacttggta
     8401 taagcggcta aattctacaa atgtaacgct agctcggtgc ggcgcacacc catgccccgg
     8461 caatctgcct cacataggtt agagggtggt caaagtggcg aacttgcgcg ctccgcgggc
     8521 gttgctctcg cgtgccgcgc ttctttttca acgtccgcga cgaggctggc ttcgccgagg
     8581 atgaggaggg gcttaacctc ggtcgggccg aagaggcgcg cgacatcgcc atcgacgggg
     8641 cgcgatcgat cctcagcgac caggcgcggc agggcagggt caacctgcgc ggccgggtcg
     8701 aagtggcgga cgcgggcggc gttccgctgt tcagcgtcga cttcggcgag gcggtcgaag
     8761 tggtggccgg cccgctcgac attcccgacc ccgcagccgc cgggcgggat ccatgatcgg
     8821 ggaagccctg gtcaagcatc tcggcctgat cggccatctc gacaaggcgg acgaagcggc
     8881 gctgctcggc atcaagggtg agattcgcga cgtggcccga gacgaggatc tgctgcgcga
     8941 gggcgagcgg ccgggcgagg cggtggtcgt actggccggg ttcctgcagc gctacacgct
     9001 gaccgcgcag ggcgagcgcc aggtccattc cttctacatg gcgaccgaca cgccgtgcct
     9061 ggaaacgctc cacatcgatt atatggacaa taatctcggc gccgtggtgc cgagccgggt
     9121 cggcgccgtc gcccatgcgg agctgttccg ggtgatggac ctgcgcccca acctgctcgc
     9181 cctcatctgg cgcgagacgc tcattcaggg cgcgatcttc cgcgaatggc tgatgcgcaa
     9241 cagccgcatg ctggcccatg tccagctcgc ccatttcttc tgcgagatgc tgacccgcgc
     9301 ccgcgccgcc ggcatcgccg acggcgacag ctgcgagctg ccgctgaccc agcaggatct
     9361 ggccgacgtg ctcggcatga ccgcggtcca cgtcaaccgc accttgatga tgctgcgctc
     9421 ggcagggttg atcgagttcc gcgcggggcg gctgatcgtg cgcaactggg acgagctggt
     9481 cgagattgcg gagttcgatc cgcattatct ccacctgcgg cgcccggctc agtagctcac
     9541 cgtcacgatc agggtctcgg cataggcgcc ggcgctgaca ttctggctgc ccgggatgcg
     9601 gccgtagaag gtgcggctgg cggcgcccct caatttggcg atgctgaagg tggcggtgcc
     9661 gccgctgccg tcgccgagca cgatcgagcg gccggcattg gtgaagagat tgtagttgag
     9721 gttgctggtg ccgttggtca tccggcgctg ggtgaaggtg ccggaccggc cggcgctgac
     9781 gctgatcacg acgtccgagt tgcgcgggca atcgacgttg atcgtgccgg cgctgtcgcg
     9841 ggcggtggcc gagcggggat tgtaggcgcc gaaggcgatg ccggcggtgc tgacgctgca
     9901 cgcgtccgcc gcggcgggga gcgcggcgag caaggccgcg gcgaggaaca ggctagcgcg
     9961 cgacatattg cggcgcctcc acgcagatga ggccgtcgat caacggctgc gggtcgtcat
    10021 tgtcgggcgc ggtcgcttcg aaggtgcagc ttttggcgcc gatgcgggcg cggaagcggc
    10081 gggtgccggc catgtcgggc acgtagatct cgccatgggt ggcgacgacc aggcgctcgt
    10141 cgctctcctc gacgccgacc tcggcgcccg ccggcaggaa gctgccgtcg gcgagggcga
    10201 cgcgaagcag cacgccgcgc tcgcgcttgg catcgaagcg gatgacggcg ccgctgcggg
    10261 cgaaggggcg aacgctggcc tcgttgcggg cgagctggac gtccatcggc aggtcggttt
    10321 cgtcgatgcg gatgcggttg tcgtcgaagg cgcgcagcgc ggggaggacc agccggccgt
    10381 cggcgccggt gcggccgatg agctggttgt cggcatagac gcgcacgccc ttatggccct
    10441 tgacctcgac cgctgcgaag ctgtcgccga ggcggcgcga ggcgaagctg tcgccggcga
    10501 tggtgccgac cgcgcccgcc gccgacagcc gcacgccggc gccgccctcg gcataagcga
    10561 cttcggcgcc gaaggcggca tattgggtct gcaggcgata ttcggcgcgg gcgctgtcga
    10621 ccgcgccggt ggtggcggcg acgcgaaagc cgctgccgtt gccgaccggc ggatcggact
    10681 ggaaggtggc cgaggcgagc gagccgcgcc ggcgatgctc gtagctcgcg ctggcgctgc
    10741 ggcggccgcc gagcggcagc acgaaatggc cgccgaagct ggtctcggat tgcccgaggg
    10801 cggtgcgccg cgcgtagagc tggagtgagc cgagccggcc gagcggcagc gtcgcgaagg
    10861 cgccgagcag ggtctcctcg ggcttgtagc gaaagtcgcg gtggaggaca ttgacgccga
    10921 ccgtcatgcg cttgaccggc agatcggcga agccctgcac ggtgagcctg gggatttcgt
    10981 agccggcggg gaggccggca taggtgtagc gcgcggtggt gagctccgag cgcaggccca
    11041 gcgacaggcc ctcggtgcgc cgctcgaacg ccgcgccgag cgcgccgccg atgccgtggc
    11101 ggccgcggct gccggcggcg gagaccgaga cgaggccgat gccgggccag gccgcggtca
    11161 ccgcgccgct cgccgcctgc cgcgaccggc tcgcctggac atggccttcg atggtgacat
    11221 ggtcggtgag gccgaagcgg tgggtggtcg aggcggagag ggcgccgtag ctgtggctct
    11281 tatagccgaa gccgcgccgc tcgaagccga gctcgtagga gaaatcgtgg aggccgcgct
    11341 ggagcatgtc ggcgccggca taataagaca ggctggtcac cacttcgcgc ccgagcaggt
    11401 cccgggtgac aacatcgacg gtgccgccgc cggagcggac cggcacgtcg gtgagctcga
    11461 acgggccggg cgttaccgtg cggctgccgc gcagcgagtt gttgacgtag atgtcgacca
    11521 cggagggcat ggcggcgctg ccctcgacgc tcggcagcgg catcgtgatg aaggcggggc
    11581 ggacggcgaa attgcggcca tattggatgc cggcgaagcg gatcggagcc gcgtgcggcc
    11641 cggcgcgggt gaccgaatcg ccgatccgga tcgaggtcag gttctccgga tcgtcgtggg
    11701 tccagttggt ttcgagccga gtgaggcggg tggcgccgcc gccaacgtcg gcgacgaagg
    11761 tggcgatgcc gacgccgtgc ggcgtgtaga ggccggcctc gaaggcgccg ccggcgcgga
    11821 gctttccgcg cgcggtctcg gcgagcagct cgtaattgag gaaggcgccg gtgccgggat
    11881 cgctcatctc gccggcggcc gggggggcga ggctcgccgt ctggcgctcg aacaggccgg
    11941 gttcggcctg cagcgcgagc cgctgctcgg cgtcggaggc ggtcagcgtc atcgcgggca
    12001 gtttgtcgac ccggcaatag ctttcgccct cgaagttggc gctctcgcag ccgccgatcc
    12061 tgaggccgct cttgccgagc aaagcggcgg cgatccaggt gccgccggca gggtcgcgaa
    12121 gcaccagcca gggttcaccc atggactggc cgttcaaggt cacttcaagc agcatcggct
    12181 gcggcgcgga ggcgagttcc gccgcctgtg ccgcgcccgg agcgatcagc agcagcagcg
    12241 gcctgagcca aagcttagtt cgccttgcgg gcgagctgct gcgggccatc gccatttcca
    12301 ggccggacga tcttctggaa ggcgatcctg tccttcacag cgggggcgct cgcgatcgtc
    12361 cagcggcggc tgccgtgcgg gagcaaaacg gcgaaatcga gcgcggggat gcggctgatg
    12421 ccggtcgcgg cgctggcctg cgcggcatcg acgcggacgt ggccggcgcc gccgttgcgc
    12481 gcttccacgg cccagcttcc gtc
//
LOCUS       DASVRK010000248         8146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_155589, whole genome shotgun sequence.
ACCESSION   DASVRK010000248 DASVRK010000000
VERSION     DASVRK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155589"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF589_09860"
     CDS             complement(<1..511)
                     /locus_tag="VF589_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HEX8257928.1"
                     /translation="MRELARLCISREWHVLAERRSSPVADWLRALARHVHEEMGGRGV
                     GAVGMCVTGNFALTMTLDAEVVAPVMAHPSFPLPIGRRKAAALHVTPETLDNARRRIR
                     EEGLKVLGLRFTGDALFCRAPRFETLRRELGDGFEAIEVPGTSARQHPEPPHSVLTIG
                     LVDEEGEPTR"
     gene            1050..1754
                     /gene="gpmA"
                     /locus_tag="VF589_09865"
     CDS             1050..1754
                     /gene="gpmA"
                     /locus_tag="VF589_09865"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX8257929.1"
                     /translation="MPRLVLIRHGQSAWNLENRFTGWWDVDLTELGVREARQAGELMA
                     ARGIDFDICFTSLQMRAIKTLNLALEAMGRLWLPVEKDWQLNERHYGGLTGLNKAEMI
                     AKAGEEQVRIWRRSFDVPPPPMEPGSPYDPSADRRYAGVTVPRTESLKDTIARVLPYW
                     EGRIAPALQRGERVLISAHGNSLRALVKHLSNIPDDEITGLEIPTGQPIVYELDGQLA
                     EVERYYLKDRPGTGDA"
     gene            1950..2699
                     /locus_tag="VF589_09870"
     CDS             1950..2699
                     /locus_tag="VF589_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8257930.1"
                     /translation="MPIPENPLELLTRTLGRHAPLSDQDRRAILSLPYTLRTLDASSY
                     TVREGDAPTHCVVLLSGFAYRQKLTGDGSRQILALHIPGEAVDFQNLFLTESDHSVQT
                     LTRAELAFIPRQQLQELARSLPAVGHAILVTTLIEASVFREWVLNVGRRDSRSRLAHL
                     LCEFAIRLETQGLADEYGYELPMTQEQLADALGLTSVHVNRTLKTLEAEGLITRNKRH
                     VSFPDWQRMRHVADFNQRYLHLHQLKPMLES"
     gene            complement(2710..2892)
                     /locus_tag="VF589_09875"
     CDS             complement(2710..2892)
                     /locus_tag="VF589_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257931.1"
                     /translation="MAYYRLSYREGGRSGASEEFEALDDIQAVQEASRRSQGEPAELW
                     CARRKVTNLPSGRAGS"
     gene            complement(2982..3563)
                     /locus_tag="VF589_09880"
     CDS             complement(2982..3563)
                     /locus_tag="VF589_09880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8257932.1"
                     /translation="MSSVSKGSADRIVQLADENALLRASLSDMRQRVAELEHVADSDT
                     VTPLPNRRRFLREIERVVGSANRHGTPSALVAIEIGNLRTINDLHGEMAGDAALIHVA
                     KLLSGLIRSTDFAARIDGDDFGLILDHLDHNSAIETAERIGRCIAENMLDLGHATVPL
                     KATVGVATILPGDCVRDVLQRAEHNMRRAKYGD"
     gene            3710..4198
                     /gene="purE"
                     /locus_tag="VF589_09885"
     CDS             3710..4198
                     /gene="purE"
                     /locus_tag="VF589_09885"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX8257933.1"
                     /translation="MADEAPPIGIIMGSRSDWETMRHAADMLDALELSYETRVVSAHR
                     TPDRLYDYAKSAAGRGLKVIIAGAGGAAHLPGMAASMTRLPVLGVPVESKALQGMDSL
                     LSIVQMPAGIPVGTLAIGKAGAANAGLLAAAILATADSALAERLDAWRQRQTDSIGET
                     PE"
     gene            4195..5262
                     /locus_tag="VF589_09890"
     CDS             4195..5262
                     /locus_tag="VF589_09890"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX8257934.1"
                     /translation="MTIAPGSTIGIVGGGQLGRMLAMAAAQLGYKCHVYAPDEAPPAA
                     EVAAHFTRGGYDDEAALARFGAEVDVATYEFENIAAGPLAALAAEAPLYPPRAALEVA
                     QDRLSEKAFVAGLGGRPAPYAGVSSREELDAALAWIGAPAILKTRRFGYDGKGQARIV
                     DPTEADAAWAAVTGAPSVLEGWIRFEAEFSILLCRSASGEIVAWDAPENVHSGGILDH
                     SSVPASPALRPAIAEAEALARKVAEALDYVGVLTLEFFAGPDGAVFNEMAPRVHNSGH
                     WTIEGATTSQFENHIRAMCGLPLGGAGLTAAEVSMQNLIGDDVERWRDILADPAARLH
                     LYGKDEARPGRKMGHVTRLGA"
     gene            complement(5291..8137)
                     /locus_tag="VF589_09895"
     CDS             complement(5291..8137)
                     /locus_tag="VF589_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8257935.1"
                     /translation="MPGCTATTRDTAPPASAAAAAPKSAAPVAELVRSIDIAYEEFTL
                     DNGLRVVVHEDRKAPVVAVSIWYNVGSKDEPKGKTGFAHLFEHLMFNGSENAPGEFFE
                     PLQQIGATDLNGTTWFDRTNYFQTVPKAAIDTILFLESDRMGHLLGAIDQKKLDNQRG
                     VVQNEKRQGDNEPYGLVEYAQLENLFPAGHPYRHSTIGSMADLDSASLADVKSWFRDN
                     YGPNNAVLVLAGDIDAAEARPLVEKYFGDIPRGPENRPAEAAVPTLPAPVEQVMKDRV
                     AATRIYRNWVVPGLLDPDVVPLDVGAAVLGGLASTRLDNEMVRKDQSAVAVSADVQEF
                     HRISLFEVKVDVKPGADAAQVAQRLDTIIADLVQNGPTEDEVQRVVTSSLADRVKGLE
                     QVGGFEGKAVALAEGALYAGEPGFYKKRLAMLAQVTPAGVQAALQKWLRRPVYALSVV
                     PGERQAYQEAGAKQGSAATHAPRYYRAPAAGEQPLAPAPRASARVDRSKMPPVGTIAD
                     LDFPDVERTTLSNGIRVAFARRAAVPMVLISVQFDAGYASDPKDRLGLQNLMASLLDE
                     GTTSLDSRELAEAQERLGADVAVGASPDRTTATLSTLRPNLAPSLDLLADIIKNPAFD
                     PGEIERLRAQQLTRIASELTQPTSIALRTLPPLLYGAEHAYGVPFTGSGRPETVKTIT
                     RDQIVAAHQSWIRPDNAEIFVVGDTSLAEITPLLEARFGAWAPPAAAKGVKSFDRSPP
                     AARPRIVLVDRPQSPQSIILAGAILPTRGTDDLLTLLTANDVLGGTFLSRLNMDLRET
                     KGWAYGVSGFVNRVEHQVPYMVYAPVQADKTGPSIVAMQEQMKAFLTRQGVTPAEFER
                     TINGNVRELPGNYETSGAVLSAIQANSLFNRPDEYQETLAGRYRAMTPAELDRAARAV
                     IRPQDLVWVVVGDAATVRPQLDKLGLPVEVMALN"
BASE COUNT         1296 a         2690 c         2854 g         1306 t
ORIGIN      
        1 cccgcgtcgg ctccccctct tcgtcaacaa ggccaatggt gagcacgctg tgcggcggct
       61 cgggatgctg cctcgcactt gtgccgggca cttcgatagc ttcaaagccg tcgccgagct
      121 cgcgtcgcag cgtctcgaaa cgaggtgcgc ggcagaaaag cgcatcgccg gtgaagcgca
      181 gcccgagcac cttgagcccc tcttcccgga tcctgcgccg cgcattgtcc aacgtctcgg
      241 gcgttacatg gagcgccgcc gcctttctcc gcccgatagg cagcgggaaa gagggatgcg
      301 ccatcaccgg cgccaccact tcggcatcaa gggtcatcgt cagggcgaag ttaccggtga
      361 cgcacatccc cacggcgccg acgccccggc cgcccatctc ctcgtgcaca tgccgcgcca
      421 gcgcccgcag ccagtccgcg accggactcg atcttctctc cgccagcaca tgccattcgc
      481 ggctgatgca gagccgggcc agctcccgca cgcggttgag ctcgttcgtc ttcttcccca
      541 ctaaccccga acaggtgcgg catgaagacc gtgaagcctg catccacgac gttgcgcgcg
      601 aaccgaagca cctcggccga gatgccaggc acttcgtgca tcacgatcac cggcggacca
      661 ttgcccttcc ggaagacggc tcgtctgcgc cccccaaaag cgaacggaat tgcaacgaag
      721 tcggcaagct cgtccattcg tggactaacg catcagcgtt gtgcttgtgg catggcttcc
      781 ttgccgctcc caagcaccgc ttccgcacct gtgtcatcgt atgatttcca cgagcgatcc
      841 gctttgccgc tccgggcgtc gaaagcagac cgaccgctac cggccaggag cgaccgcttg
      901 aaaacaccaa gccgaatgtc cgcatgggtc gaaagcggac attccgcctt tcatcaaatt
      961 tcaaaccgac ccactacccg ccggcggcca aggttgacat ggcccggcac gggcgtgagg
     1021 gcggcgggac gccaaagacg gagagttaga tgccccgcct cgtcctgatc cgccacggcc
     1081 agtcggcctg gaatctggag aatcgcttca ccggatggtg ggacgtcgat ctcaccgagc
     1141 ttggcgtgcg cgaggcgcgg caggcgggcg agctgatggc ggcgcgcggg atcgacttcg
     1201 acatctgctt caccagcctc cagatgcggg cgatcaagac gctcaacctg gcgctcgagg
     1261 cgatggggcg gctgtggctg ccggtcgaga aggactggca gttgaacgag cgccattatg
     1321 gcgggctcac cggcctcaac aaggccgaga tgatcgccaa ggcgggcgag gagcaggtca
     1381 ggatctggcg gcgctcgttc gacgtgccgc cgccgccgat ggagccgggc agcccgtatg
     1441 atccatcggc cgaccgccgc tatgccggcg tgacggtgcc gcgcaccgaa agcctcaagg
     1501 atacgatcgc gcgcgtgctg ccttattggg aggggcgaat cgcgccggcg ctgcagcgcg
     1561 gcgagcgggt gctgatctcc gcccacggca attcgctcag ggcgctggtc aagcatctgt
     1621 ccaacatccc cgacgacgag attaccggcc tcgaaatccc gaccggccag ccgatcgtct
     1681 acgagctgga cgggcaactg gcggaggtgg agcgctatta tctgaaggac cgccccggca
     1741 ccggcgacgc ctgatctggc cgcggccgac cgctaagctt gccaaacgcc gtccgccgac
     1801 cgaacctatg ttaagacgcc acttctatcc taagatagga aaccgcctcg gcacctgtgt
     1861 tagtgtctcc ccatcgccgg gttgaatatt cgggccccgg cggccgagat gcttcgtgct
     1921 cccgcccatc tttgcgcagg agacgaatgt tgcctatccc cgaaaacccg ctggaattgc
     1981 tgacgcggac gctggggcgc cacgccccgc tgagcgacca agaccgccgg gcgatcctgt
     2041 cgctgcctta taccctgcgc accctcgacg cttcctccta caccgtccgc gaaggcgacg
     2101 cgccgaccca ttgcgtcgtg ctgctctccg gcttcgccta tcgccagaag ctgaccggcg
     2161 acggctcgcg ccagatcctg gcgctgcaca taccgggcga agcggtcgac tttcagaacc
     2221 tgttcctcac cgaatcggac cacagcgtcc agacgctgac gcgcgccgag ctcgccttca
     2281 ttccgcgcca gcaattgcag gagctggccc gcagcctgcc cgctgtcggc catgcgatcc
     2341 tggtcacgac cctgatcgaa gcttcggtgt tccgggaatg ggtgttgaat gtcgggcggc
     2401 gcgattcgcg gtcgcggctg gcgcacctat tgtgcgagtt cgcgatccgc ttggagacgc
     2461 aggggctggc ggacgaatat ggctatgagc tgccgatgac gcaggagcag ctggcggatg
     2521 cgctcggcct cacctcggtg cacgtcaacc gcacgctgaa gacgctggag gcggaaggcc
     2581 tcatcacgcg caacaagcgc catgtcagct tccccgactg gcagcggatg cggcacgtgg
     2641 cggacttcaa ccagcgctat ctgcatctcc atcagctgaa gccgatgctg gaaagctgag
     2701 ccctttcgct caggagccgg cgcggccgga cggcaggttg gtcaccttgc gcctggcgca
     2761 ccacaattcg gccggctcgc cctggctccg gcgcgacgcc tcctggaccg cctggatgtc
     2821 gtccagtgcc tcgaactcct cggacgcgcc cgaccggccg ccctcccgat aggaaagcct
     2881 gtaataagcc atggaaaccc ccggccgctt ccatagcagc ttgggcggcc cttttccacg
     2941 cgggcgctga taacctggat cagaggacgg ggaggtgccg gtcagtcgcc atatttggcg
     3001 cggcgcatat tgtgctcggc gcgctggagc acgtcgcgga cgcagtcgcc ggggaggatg
     3061 gtggcgacgc cgacggtggc cttgagcggc accgtggcgt ggccgagatc gagcatgttc
     3121 tcggcgatgc agcggccgat ccgttcggcg gtctcgatcg cgctgttgtg atcgagatgg
     3181 tcgaggatca ggccgaaatc gtcgccgtcg atgcgggcgg cgaaatcggt cgagcgaatc
     3241 aggccggaca gcaatttggc gacgtggatg agcgcggcat cgcccgccat ctcgccatga
     3301 aggtcgttga tggtgcgcag attgccgatc tcgatggcta ccagggccga cggggtgccg
     3361 tggcggttgg ccgaacccac cacccgctcg atctcgcgca ggaagcggcg gcggttgggg
     3421 agcggggtga cggtgtcgct gtcggcgaca tgttcgagct cggcgacgcg ctggcgcatg
     3481 tcgctgagcg aggcgcggag cagcgcattc tcgtcggcga gctggacgat gcgatccgcc
     3541 gatcccttgc tgactgagct catattccgt tccgcatcgc tggcatgacc gggcaagcta
     3601 tagccgcaag cggttaacag aacgccaaat cgggaggcgg cccgcgcgct atttgcctag
     3661 cgccgcctcc ccgcttaaga ggtgctgctt cgccgcgcag aaggacagga tggcagacga
     3721 agcccccccg atcggtatca tcatgggcag ccgctccgat tgggagacga tgcgccatgc
     3781 cgccgacatg ctcgacgcgc tcgagctaag ctacgagacg cgggtggtgt cggcgcaccg
     3841 cacgcccgac cggctctacg actatgccaa aagcgcggcc gggcgcgggc tgaaggtgat
     3901 catcgcgggc gcgggcgggg cggcgcattt gccgggcatg gcggcgtcga tgacccggct
     3961 gccggtgctc ggcgtgccgg tcgaatcgaa ggcgctccag ggcatggaca gcctgctgtc
     4021 gatcgtccag atgccggccg gaatcccggt tgggacgctt gccatcggca aggccggggc
     4081 ggccaatgcg ggcttgctcg cggccgccat cctcgccacc gccgacagcg cgctcgccga
     4141 acggctcgac gcctggcggc agcggcagac ggacagcatc ggcgaaacgc ctgaatgacg
     4201 atcgcgccgg gctccaccat cggcatcgtc ggcggcggcc agctcgggcg gatgctggcg
     4261 atggcggcgg cgcagctcgg ttataaatgc cacgtctacg cgcccgacga agcgccgccg
     4321 gcggccgagg tggcggcgca tttcacgcgc ggcggctatg acgacgaagc cgcgctggcc
     4381 cgattcgggg cggaggtcga cgtcgccacc tatgaattcg agaatatcgc cgccgggccg
     4441 ctcgccgcgc tggccgccga ggcgccgctc tacccgcccc gggcggcgct ggaggtggcg
     4501 caggaccggc tcagcgagaa agcgttcgtg gcgggcctcg gcggccggcc ggctccttat
     4561 gcgggcgttt cgagccgcga ggagctggac gcggcgctgg cgtggatcgg agcgccggcg
     4621 atcctcaaga cccggcgatt cggctatgac ggcaagggcc aggcgcggat cgtcgatccg
     4681 accgaggccg acgccgcctg ggccgcggtg acgggcgcgc cgtcggtgct cgagggctgg
     4741 ataaggttcg aggcggaatt ctcgatcctg ctgtgccggt cggcgagcgg cgagatcgtg
     4801 gcgtgggacg cgccggagaa tgtccattcc ggcggcatcc tcgatcattc gagcgtgccc
     4861 gcctcgcctg ccctgcggcc ggcgatcgcc gaggccgagg cgctggcgcg caaggtcgcc
     4921 gaggcgctcg attatgtcgg cgtgctgacg ctcgaattct ttgccggccc ggatggagct
     4981 gtgttcaacg aaatggcgcc gcgggtgcac aatagcggcc attggacgat cgagggggcg
     5041 acgacctcgc aattcgagaa ccatatccgc gccatgtgcg gcctgccgct gggcggcgcc
     5101 gggctgaccg cggcggaggt gtcgatgcag aatttgatcg gcgacgacgt cgagcgctgg
     5161 cgcgacatcc tcgccgatcc ggccgcccgg ctccacctct acggcaagga cgaggcccgg
     5221 cccggccgca agatggggca tgtgacgcgg ctcggcgcct gaggtctggg ctcggggccg
     5281 cagccgaaga tcagttcagc gccatcacct cgaccgggag gccgagcttg tcgagctggg
     5341 gccggacggt ggcggcgtcg ccgaccacca cccacaccag atcttgcggc cggatcacgg
     5401 cgcgggcggc gcggtcgagc tcggccgggg tcatcgcccg gtaccggccg gccagcgttt
     5461 cctgatattc gtcggggcgg ttgaacagcg aattggcctg gatggcggac agcaccgccc
     5521 cggacgtctc gtagttgccc ggcagctcgc ggacgttgcc gttgatcgtg cgctcgaact
     5581 cggcgggggt aaccccctgg cgggtgagga acgccttcat ctgctcctgc atggcgacga
     5641 tcgaggggcc ggtcttgtcg gcctggaccg gcgcgtagac catgtagggg acctggtgct
     5701 cgacccggtt gacgaagccg gacacgccat aggcccagcc cttggtctcg cgcaggtcca
     5761 tgttgaggcg cgagaggaag gtgccgccga gcacgtcatt ggcggtgagc agggtcagga
     5821 ggtcgtcggt gccgcgggtc ggcaggatcg cgccggcgag gatgatcgac tgcggcgatt
     5881 gcggccggtc gaccagcacg atccgggggc gcgcggccgg cggcgagcgg tcgaagctct
     5941 tgacgccctt cgccgcggcc ggcggcgccc aggcgccgaa gcgggcctcg agcaaaggcg
     6001 tgatctcggc gaggctggtg tcgccgacca cgaaaatctc ggcattgtcg ggccggatcc
     6061 agctttgatg cgcggcgacg atctggtcgc gggtgatggt cttgaccgtc tcgggccggc
     6121 cggagccggt gaacggcacc ccataagcat gctcggcgcc gtagagcaaa ggcggcaaag
     6181 tgcgcagcgc gatgctggtc ggctgggtga gctcgctggc gatgcgggtc agctgctggg
     6241 cgcgcagccg ctcgatctcg cccgggtcga aggccgggtt cttgatgatg tcggcgagca
     6301 ggtcgagcga aggggcgagg ttcggccgca aagtagacaa ggtggcggtg gtgcggtccg
     6361 gcgaggcgcc gaccgcgacg tcggcgccga gccgctcctg cgcctcggcc agctcgcgcg
     6421 aatcgaggct ggtggtgccc tcgtcgagca atgacgccat caaattttgg aggccgagcc
     6481 ggtccttggg atcggaggcg tagccggcat cgaactggac cgagatcagc accatcggca
     6541 ccgccgcccg gcgggcgaag gcgacgcgga tgccgttcga gagggtggtg cgctcgacat
     6601 cggggaaatc gaggtcggcg atggtgccga ccgggggcat tttggagcgg tcgacgcggg
     6661 cggaggcgcg cggggcgggc gccagcggct gctcgcccgc ggccggagcg cgatagtaac
     6721 gcggcgcatg ggtcgcggcg gagccttgct tggcccccgc ctcctgatag gcctggcgct
     6781 cgcccggcac gacgctcagc gcatagaccg ggcgcctcag ccacttctgc agcgcggcct
     6841 ggacgccggc cggcgtcacc tgcgccagca tcgccagccg tttcttgtaa aagccgggtt
     6901 cgcccgcata gagcgcgccc tcggcgagcg cgaccgcctt gccctcgaaa ccgccgacct
     6961 gctcgagccc cttgacgcga tcggcgaggc tgctggtgac gacgcgctgg acctcgtcct
     7021 cggtggggcc gttctggacg agatcggcga tgatcgtatc gagccgctgc gcgacctggg
     7081 ccgcgtcggc gcccggcttc acgtcgacct tgacttcgaa caggctgatg cggtggaatt
     7141 cctggacgtc ggcgctgacg gcgacggcgg actggtcctt gcggaccatt tcgttgtcga
     7201 gccgcgtgct ggcgaggccg ccgagcaccg ccgcgccgac gtcgagcggg accacgtccg
     7261 gatcgagcag gcccggcaca acccaattgc ggtagatgcg ggtggcggcg acgcggtcct
     7321 tcatcacctg ctcgaccggc gccgggaggg tcggcaccgc cgcctcggcc ggccgattct
     7381 ccggcccgcg cgggatgtcg ccaaaatatt tctcgaccag cggccgcgcc tcggcggcat
     7441 cgatgtcgcc ggccagcacc agcaccgcat tgttggggcc gtaattgtcg cggaaccagc
     7501 tcttcacgtc ggcgaggctc gccgaatcga gatcggccat cgaaccgatc gtcgagtggc
     7561 gataggggtg cccggccggg aacaggttct cgagctgggc atattcgacc aggccgtagg
     7621 gctcgttgtc gccctgccgc ttctcgttct ggacgacgcc gcgctggtta tcgagcttct
     7681 tctggtcgat cgcgccgagc agatggccca tgcggtcgct ttcgaggaac aggatggtat
     7741 cgatcgccgc cttcggcacg gtctggaaat aattggtgcg gtcgaaccag gtggtgccgt
     7801 tgaggtcggt ggcgccgatc tgctggagcg gctcaaagaa ttcgcccggc gcattctcgg
     7861 agccgttgaa catcagatgc tcgaacagat gggcgaagcc ggtcttgccc ttcggctcgt
     7921 ccttggagcc gacattgtac cagatgctga ccgccaccac cggcgccttg cggtcctcgt
     7981 ggacgacgac gcgcaggccg ttatcgagcg tgaattcctc ataggctatg tcgatcgagc
     8041 gcacgagctc ggcgaccggc gcggccgact tgggcgcggc tgccgcggcc gaggcgggcg
     8101 gcgccgtatc gcgcgtggtt gcggtgcagc cgggcagggc caggcc
//
LOCUS       DASVRK010000249         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_424375, whole genome shotgun sequence.
ACCESSION   DASVRK010000249 DASVRK010000000
VERSION     DASVRK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424375"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VF589_09900"
     CDS             <1..277
                     /locus_tag="VF589_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257936.1"
                     /translation="LPIWRVDFGDEGNSSFYVSGTTGKLLERRNDSWRLWDFFWMLHN
                     MDYANRASFNHPLIVAVTFGILWLTITGVWLLFKTGWRAERRLLRRP"
     gene            complement(274..444)
                     /locus_tag="VF589_09905"
     CDS             complement(274..444)
                     /locus_tag="VF589_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257937.1"
                     /translation="MNIRCLLGRHKPINDLSDWRHGYYLSACADCGTPMRYYGGHWEA
                     HRTRRKSGQDPG"
     gene            complement(514..2628)
                     /locus_tag="VF589_09910"
     CDS             complement(514..2628)
                     /locus_tag="VF589_09910"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase"
                     /protein_id="HEX8257938.1"
                     /translation="MAAVQGNVRSGDRDVDAMLEGFRWSASMLTFSFPTSAGQYGGAY
                     GPGDEPYDNFQAFSSSQQLRAREVLASFSSVAMLDYDEVSGGGGDLRFGMTDDTAAAH
                     AYTPGPDARAGDTWYGNSSGWYDDPRVGNYGYHALLHEVGHAVGLKHPHETHQFGAVS
                     GAHDWMAYSVMSYRSYEGQSLDGGFSNEENGYSQSLMMYDIAALQHMYGARFDTVPVD
                     TVYHWTPGSSQMLINGVGQPGSVTNRIFRTVWDGGGNDTYDLSAYTTGMRISLEPGKW
                     SELSAAQLPSLSGMIHPPGNVANALLHRGDTRSLIENAIGGSGNDEITGNAGANRLDG
                     GAGADRLSGGFGDDVYIVDDLRDEIVSEATVARGGTDTVESSVHFTLGTGVEKLVLTG
                     AAATRGEGNSLDNVITGNGAANYLKSGLGADLLVGGGGNDRYFIHDADDRVLEVAGGG
                     VDHVLSQVDFRLSEGVETLELSGPARAGTGNAGANRIVGTAAANRLDGGAGIDVLIGG
                     GADDLYVFDHARDRAVERADGGFDTVLSSVGHILRAEVEALALVGRAALAGTGNALAN
                     SLTGNHGANRLDGAEGDDRLVGGRGNDLLTGGEGRDAFCFDSALNRFNNVDELIDYSR
                     IDDTIYLDCDVFTALAEGKIAAEAFHRGAAAADAGDRILFDQASGKLFYDADGNGDGA
                     AILFATLAPGASVNSFDFVGYI"
BASE COUNT          488 a          964 c          975 g          498 t
ORIGIN      
        1 gctcccgatc tggcgcgtcg acttcggcga cgagggcaac agcagcttct acgtctccgg
       61 aaccaccggc aagctgctcg agcgccgcaa cgacagctgg cggctgtggg acttcttctg
      121 gatgctccac aatatggatt atgcgaaccg ggccagcttc aaccacccgc tgatcgtcgc
      181 ggttacgttc ggaatcctgt ggctgacgat caccggcgta tggctgctgt tcaagaccgg
      241 ctggagggcg gagcgccgcc tgctgcgccg gccctaaccc ggatcctggc cggacttgcg
      301 gcgcgtccga tgggcctccc agtggccgcc ataatagcgc atcggcgtgc cgcaatcggc
      361 gcaggcgctg agataatagc cgtggcgcca gtccgacagg tcgttgatcg gcttgtggcg
      421 gccgagcagg catctgatgt tcatggcggc atcttagcag atcacttcgc gccataggaa
      481 gcggcccgtc agcaggggct cggccggacc gtcctagata tagccgacga agtcgaacga
      541 gttgaccgac gctccgggcg cgagggtggc gaacaggatc gccgcgccat cgccattgcc
      601 gtcggcatcg tagaacagct tcccgctcgc ctggtcgaac aggatgcggt cgccggcatc
      661 ggcggccgcg gcgccgcgat ggaaggcctc ggcggcaatc ttgccttccg ccagcgcggt
      721 aaacacgtcg cagtccagat agatggtgtc gtcgatccgc gaataatcaa tgagctcgtc
      781 gacattgttg aagcggttga gcgcgctgtc gaagcagaaa gcgtcgcggc cctcgccgcc
      841 ggtcaggaga tcgttgccgc gtccgccgac aagacggtcg tcgccttccg cgccgtcgag
      901 gcggttggcg ccgtgattgc cggtgaggct gttggcgagg gcgttgccgg tgccggcgag
      961 cgccgcccgc ccgaccagcg ccagcgcctc gacctccgcg cgcaatatgt ggccgacgct
     1021 gctgaggacg gtgtcgaacc cgccgtccgc ccgttcgacg gcgcggtcgc gggcatggtc
     1081 gaacacataa agatcgtccg cgccgccgcc gatcagcacg tcgatgccgg cgccgccgtc
     1141 gagccggttg gcggcggccg tgccgacgat gcggttcgcc ccggcattcc cggtgccggc
     1201 tcgcgccggg ccagacagct cgagagtctc gaccccctcg ctcaagcgga aatccacctg
     1261 gctcagcaca tggtcgacgc cgccgccggc gacttcgagg acccggtcgt ccgcatcatg
     1321 aatgaaatag cggtcattgc cgccgccgcc caccagcaga tcggctccga ggcccgactt
     1381 gaggtaattg gcggcgccat tgccggtgat gacattgtcg agggaattgc cctccccccg
     1441 cgtcgccgct gcgccggtga gcacgagctt ctcgactccg gtaccgagcg tgaagtgcac
     1501 cgacgattcg accgtgtcgg tgccgccccg cgcgaccgtc gcctccgaca caatttcgtc
     1561 ccgaaggtcg tcgacgatgt acacatcgtc gccgaagccg ccgctcagcc ggtcggcgcc
     1621 cgcgccgcca tcgagccggt tggcgccggc attgccggtg atctcgtcat tgcccgaccc
     1681 gccgatcgca ttttcgatca gcgagcgggt gtcgccgcgg tggagcaagg cgttggcgac
     1741 gttgcccggc gggtggatca tcccagacag gctggggagc tgggcggccg acaattcgga
     1801 ccatttcccc ggttccaggc tgatccgcat gccggtcgtg taggccgaca gatcgtaggt
     1861 gtcgttgccg ccgccgtccc agacggttcg gaagatccga ttcgtaaccg aacctggctg
     1921 gccgacccca ttgatgagca tctggctcga gccgggcgtc cagtgataga ccgtgtccac
     1981 cggcacggtg tcgaaccggg cgccgtacat gtgctggagc gccgcaatgt cgtacatcat
     2041 gaggctttgc gaatagccat tttcttcgtt ggagaagccg ccgtcgagcg actgcccctc
     2101 ataggagcgg tagctcatca ccgaataggc catccagtcg tgggccccgc tcaccgcgcc
     2161 gaactgatgg gtctcgtgcg ggtgcttgag accgaccgcg tggccgacct cgtggagcag
     2221 cgcgtgatag ccgtaattgc cgacgcgcgg atcatcgtac cagcccgagc tgttgccgta
     2281 ccaggtgtcg cccgcgcggg cgtcgggacc gggcgtatag gcatgggcgg ctgcggtgtc
     2341 gtcggtcatg ccgaagcgaa ggtcgccgcc gccgccgctc acctcgtcat agtcgagcat
     2401 cgccaccgag gaaaaggatg cgaggacctc gcgggcgcgc aattgctggc tggagctgaa
     2461 ggcctggaaa ttatcgtaag gctcgtcgcc cgggccatag gctccgccat attggccggc
     2521 ggaagtgggg aagctgaagg tcagcatgga tgccgaccag cggaaccctt ccagcatggc
     2581 atcgacatcg cggtcgccgg acctgacatt gccctgaacc gccgccatgc ccatctcccc
     2641 gctcgtcgtc gatccggccc gagcctataa cgtcgagaca tggctgctta ctgaataagc
     2701 ctttagaaaa caacaggaag ctgttggcgt gaaccgatag cgtccgcgcc tcgggaggcg
     2761 cagacgctcg gtaggcggga cgccggccgg ctcagccgta ggcgttgaag tcggcgttgg
     2821 tgaggtcgct gccgggatcg acgcgggcga acagcaccgc tgcctggccg ccgctgccat
     2881 cggcgtcata gaagatccgg ccggtaccct cgtcatagag gatcc
//
LOCUS       DASVRK010000250         5134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_155849, whole genome shotgun sequence.
ACCESSION   DASVRK010000250 DASVRK010000000
VERSION     DASVRK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155849"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2769)
                     /locus_tag="VF589_09915"
     CDS             complement(<1..2769)
                     /locus_tag="VF589_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX8257939.1"
                     /translation="MNFISIAFDDLNAFAFLKELYGGTLVTPNIDRVMAMGTTFDNAF
                     SQVAICNASRTSVLTGQAPGLTGVHANYETWHEYVDASATLPAMLKGGGYHTSVIGKV
                     FHELAVAPEVAGIVADYTFGNGDNRFFENGLFGTHPLGTAPETHGDHINVDKAIELLN
                     AAGADPFAMFLGLSKPHLSWVVPQQYFDLYPLDSIELPFHLDGDLSDVPEFIKGIIHE
                     AEHAEVLSADYWKAALQGYFASISFADAMLGRVLDTLEANGQLDTTAILLWTDHGYHL
                     GDKDTWHKFTLWEEAARAPFVLALPGAADDAQHVKQVVELVDIVPTVLDLLGMDAPAG
                     LSGRSLLPFVADPALIDDAAAVTTMYGSAALRTNNYRYIRYEDGSTELYDIVADPNQW
                     TNLAGDPALQAVRDGLDARLRAELGADGWVLVDPGQGHVGTDAAQLFVLAPGHGEVAG
                     GGGDDSYFVSSGVSPIVELEGGGHDTVYVATDYVMPDHIEELQIKANYGRLVLTGNGS
                     DNIMVGGGRLYGMGGADAIRGTGGKDVLDGGAGDDHLEGYAGDDVIAGGDGNDRIDGA
                     SGHDTASYAGAASAVTVALGPLGAQDTSGAGIDTLTFIEGLAGSGWDDVLTGNGVANS
                     IDGGAGSDLIEGGAGDDRLGGGSGVDTVSYGSAEAAVEVSLAVAAAQATGGAGTDTLA
                     SFENIFGSAYNDKLTGNDGVNLISGGAGDDRLDGGLGYDTISYASAGAGVSVSLADAG
                     PQQTGGAGIDTLAGFERIAGSAWNDRLAGDGGSNRIDGGGGDDEVDGGGGSDELAGGD
                     GIDRLSFASAASGVTASLALAARQNTRGGGFVTLSGFETIVGSAWADRLTGSGEANIL
                     DGGQSEDVLAGGGGDDVYILDHAADVVTEAAGEGSDEVRTGLSAHTLADNVEILTGTA
                     SAGQALTGN"
     gene            2968..5034
                     /locus_tag="VF589_09920"
     CDS             2968..5034
                     /locus_tag="VF589_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX8257940.1"
                     /translation="MATTTMGGTALKWAPWALVALVGAMALGVVALHRGETINALWIV
                     VAALCVYLTAYRFYAMFIAERVLGIDPSRRTPAVRRNDGLDYVPTNRTVLFGHHFAAI
                     AGAGPLVGPVLAAQMGYLPGTLWILAGVVFAGAVQDFIMLFISTRRDGGSLGEIIRGE
                     MGNVPGVIAMIGILAIMIILLAVLALVVVKALADSPWGTFTVFATMPVALLMGLYGRY
                     IRPGRILEVSLIGFVGLMLAIIFGESVAASPVWGPAFTFSGEALALMLIAYGFVASVI
                     PVWLLLAPRDYLSTFLKIGTILGLAIGILIVQPELKMPAVTPFIDGSGPVFAGNLFPF
                     LFITIACGAVSGFHALVASGTTPKMIESEAHLPVIGYGAMLMESFVAIMALVAASVLD
                     PAVYFALNSPAAAIGVTTAEAAQAVSVWGFAITPSALDQVAIDVGESTILSRTGGAPT
                     LAVGMAQILSNVIGGPAMMAFWYHFAILFEALFILTTVDAGTRVARFMIQDLLGTAFA
                     PLKDTKSWTANLVATAIAVGGWGWFLYQGVTDPLGGINTLWPLFGISNQMLAAIALTL
                     CTVVLFRMKRERYAWVTLLPLTWLLICTLTAGWQKMFHADPRIGFLAHASNYSSALAR
                     GEILAPAKSLGEMRRVIFNDYVNASLCALFIAVVLAMVFYGVRAIAAARRSETPTVRE
                     VADALA"
BASE COUNT          733 a         1812 c         1683 g          906 t
ORIGIN      
        1 gttgccggtc agcgcctggc cggcgctcgc ggtgccggtc agaatctcga cattgtcggc
       61 gagcgtgtgc gcggaaaggc cggtgcggac ctcgtcggag ccctcgccgg ccgcttcggt
      121 caccacgtcg gcggcatggt cgaggatata gacatcgtcg ccgccgccgc cggccagcac
      181 gtcctcgctt tggccgccgt ccaggatatt ggcctcaccg gagccggtca gccggtccgc
      241 ccaggccgag ccgacgatcg tctcgaagcc tgacagggtg acgaagccgc cgccgcgcgt
      301 gttctggcgc gccgccaagg cgaggctcgc ggtgacgccg ctggcggcgg aggcgaagga
      361 gaggcggtcg atgccgtcgc ccccggccaa ctcgtcgctg ccgccgccgc cgtcgacttc
      421 atcgtcgccg ccgccgccgt cgatgcggtt ggagccgccg tcgccggcga ggcggtcgtt
      481 ccaggccgag ccggcaatcc gctcgaaccc ggcgagcgtg tcgatgccgg cgccgcccgt
      541 ctgctgcggg cccgcgtcgg cgaggctcac cgacacgccc gcgccggccg aggcatagga
      601 gattgtgtcg tagccgagcc cgccgtcgag gcggtcgtcg ccggcgccgc cgctgatcag
      661 attgacgccg tcattgccgg tgagcttgtc gttataggcc gagccgaaga tgttctcgaa
      721 gcttgcgagg gtgtcggtgc cggcgccgcc ggtcgcctgc gcggccgcca cggccaggct
      781 gacttcgacc gcagcctcgg ccgagccgta ggacacggtg tcgacgccgc tgccgccgcc
      841 gagccggtcg tcgccagcgc cgccttcgat aagatcggag ccggcgccgc cgtcgatgct
      901 gttggcgacg ccattgccgg tcagcacatc gtcccagccg gatccggcca gcccctcgat
      961 gaaggtcagc gtgtcgatgc ccgccccgct tgtgtcctgc gccccgagcg ggccgagcgc
     1021 gaccgtcacc gcggacgcgg cgccggcata agaggcggtg tcgtggccgg aggctccgtc
     1081 gatgcggtcg ttgccgtcgc cgccggcgat cacgtcgtcg ccggcatagc cttcgagatg
     1141 gtcgtcgccc gcgccgccgt cgagcacgtc cttgccgccg gtgccgcgaa tggcgtccgc
     1201 gccgcccatg ccgtaaagcc ggccgccgcc gaccatgata ttgtcggaac cgttgccggt
     1261 cagcaccagc cggccgtaat tggccttgat ctggagctcc tcgatatggt cgggcatgac
     1321 atagtcggtg gcgacataga cggtgtcgtg gccgccgccc tccagctcga cgatcggcga
     1381 gaccccgctg ctgacgaaat agctgtcgtc gccgccgccg ccggccactt cgccatggcc
     1441 gggcgcgagc acgaacaatt gcgcggcgtc ggtgccgaca tggccctggc cgggatcgac
     1501 gagcacccag ccgtcggcgc cgagctcggc cctgagcctc gcgtcgagcc cgtcgcggac
     1561 agcctgcagc gccggatcgc cggcgaggtt ggtccactga ttcgggtcgg cgacgatgtc
     1621 gtaaagctcg gtcgacccgt cctcgtagcg gatgtagcga tagttattgg ttctgagcgc
     1681 cgcgcttccg tacatggtcg tcacggcggc ggcatcgtcg atcagcgccg gatcggcgac
     1741 gaagggcagc agcgagcggc cggacagccc ggcaggggcg tccatcccga gcaggtcgag
     1801 cacggtaggc acgatgtcga ccagctccac cacctgcttc acatgctgcg catcgtcggc
     1861 cgcgcccggc agcgccagca cgaacggcgc gcgggcggcc tcctcccaca aggtgaattt
     1921 gtgccaggtg tccttgtcgc cgaggtgata gccatggtcg gtccacagca ggatcgcggt
     1981 ggtgtcgagc tggccgttcg cctccaaggt gtcgagcacc cgcccgagca tcgcgtcggc
     2041 aaaggagatg ctggcgaaat agccctgcag cgccgccttc caataatcgg cgctgagcac
     2101 ctcggcatgc tcggcctcgt ggatgatccc cttgatgaat tcggggacgt ccgaaaggtc
     2161 gccgtcgaga tggaacggca gctcgatcga gtcgagcgga tagaggtcga aatattgctg
     2221 cgggacgacc cagctcagat gcggcttgga aaggccgagg aacatcgcga acgggtcggc
     2281 cccggccgcg ttcagcagct cgatcgcctt gtcgacgttg atatggtcgc catgggtttc
     2341 gggcgcggtc ccgagcgggt gcgtgccgaa caggccgttc tcgaaaaatc ggttgtcgcc
     2401 gttgccgaac gtatagtcgg cgacgatgcc ggccacttcg ggcgcgaccg ccagctcgtg
     2461 gaacaccttg ccgatgacgc tggtatgata gccgccgccc ttgagcatgg ccgggagggt
     2521 cgccgacgcg tcgacatatt cgtgccaggt ctcgtaattg gcatggacgc cggtgaggcc
     2581 cggcgcctgg ccggtcagca ccgacgtgcg cgaagcgttg cagatcgcga cctgggagaa
     2641 ggcgttgtcg aacgtcgtcc ccatcgccat cacccggtcg atgttgggcg tgacgagcgt
     2701 gccgccgtac aattccttga ggaaggcgaa ggcgttgagg tcgtcgaagg cgattgaaat
     2761 gaagttcatg cgccccctcg gcccgccaaa ccctggccct gccccgatac cccttaaggc
     2821 tttcgaaatc gagaaaaaac ttaatgcagg ctatggctta gctatcccag caacgccagt
     2881 ggcttggggg cttgcccgcc cgccctccgg atcgctagca ataggggctc cggcaagggc
     2941 ggccggcggg ctgcggcgga gaggcagatg gcgacgacga cgatgggcgg caccgctttg
     3001 aaatgggcgc catgggcgct ggtcgccctg gtcggggcaa tggctttggg cgtggtcgct
     3061 ttgcaccgcg gcgagaccat caacgcgctc tggatcgtgg tcgccgcgct ctgcgtctac
     3121 ctcaccgcct accgtttcta cgcgatgttc atcgccgaac gcgtgctcgg catcgatccc
     3181 tcgcgccgca ctcccgccgt ccggcgcaac gacggcctcg actacgtgcc gaccaaccgc
     3241 accgtcctgt tcggccacca tttcgcggcc atcgccggcg ccggtcccct ggtcggtccg
     3301 gtgctggcgg cgcagatggg ctatcttccc ggtactttgt ggatcctggc cggcgtcgtc
     3361 ttcgccggcg cggtccagga tttcatcatg ctgttcatct ccacccgccg cgacggcggt
     3421 tcgctcggcg agatcatccg cggcgagatg ggcaacgtgc ccggcgtgat cgcgatgatc
     3481 ggcatcctgg cgatcatgat catcctcctc gccgtgctcg ctttggtcgt cgtcaaggcg
     3541 ctcgccgaca gcccatgggg caccttcacc gtgttcgcga ccatgccggt ggccctcctg
     3601 atgggcctct acggccgcta catccggccc ggccgcatcc tcgaagtgtc gctgatcggc
     3661 tttgtcgggc tgatgctcgc catcatcttc ggcgagagcg tcgccgccag cccggtctgg
     3721 gggcccgcct tcaccttctc gggcgaagcg ctcgctttga tgctgatcgc ttacggcttc
     3781 gtcgcctcgg tgatcccggt ctggctgctg ctggcgccgc gtgactatct ctcgaccttc
     3841 ctcaagatcg gcaccatcct cggcctcgcc atcggcatcc tcatcgtcca gcccgagctc
     3901 aaaatgccgg ccgtcacccc tttcatcgac ggcagcggcc cggtgttcgc cggcaacctc
     3961 ttcccgttcc tgttcatcac catcgcctgc ggcgcggtgt cgggcttcca cgctctggtc
     4021 gcctccggca ccacgcccaa gatgatcgag agcgaggccc atttgcccgt gatcggctat
     4081 ggcgcgatgc tgatggaaag cttcgtcgcg atcatggcat tggtcgcggc gagcgtgctc
     4141 gatcccgccg tctatttcgc cttgaacagc ccggccgccg caatcggcgt caccaccgcc
     4201 gaggcggcgc aggcggtctc ggtctggggc ttcgcgatca cgccctcggc gctcgaccag
     4261 gtcgcgatcg acgtcggcga atcgaccatc ctcagccgca ccggcggcgc gcccacgcta
     4321 gcggtcggca tggcccagat cttgtcgaac gtgatcggcg ggccggcgat gatggccttc
     4381 tggtaccatt tcgcgatcct gttcgaggcc ttgttcatcc tcaccaccgt cgacgccggc
     4441 acccgcgtcg cccgcttcat gatccaggac ctgctcggca ccgccttcgc gcccttgaag
     4501 gacaccaaga gctggaccgc caacctcgtc gccaccgcca tcgccgtcgg cggctggggc
     4561 tggttcctct atcagggcgt caccgatccc ttggggggca tcaacacgct gtggcctttg
     4621 ttcggcattt ccaaccagat gctcgccgcg atcgcgctca ccttgtgcac cgtcgtgctg
     4681 ttccggatga agcgcgagcg ttacgcctgg gtgaccctgc tcccgctgac ctggctgctc
     4741 atctgcacgc tcaccgccgg ctggcagaag atgttccacg ccgatcccag gatcggcttc
     4801 ctcgcccacg cctccaatta ttcgtccgcc ctcgcccgcg gcgagatcct cgccccggcc
     4861 aagagcctcg gcgagatgcg ccgcgtcatc ttcaacgatt atgtcaatgc gagcctctgc
     4921 gccctcttca tcgcggtggt gctggcgatg gtcttctatg gcgtgcgcgc catcgccgcc
     4981 gcccgccgct ccgaaacgcc gaccgtgcgg gaggtcgccg atgcgctggc gtgacgcgct
     5041 cgcccgcgcc gccgagaccg cgcggctgat ggtcggcgtc cccgattacg acgcctaact
     5101 cgcccaccgc cgcgcccatc atcccggcga gccc
//
LOCUS       DASVRK010000251         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_599395, whole genome shotgun sequence.
ACCESSION   DASVRK010000251 DASVRK010000000
VERSION     DASVRK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599395"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VF589_09925"
     CDS             <1..687
                     /locus_tag="VF589_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257941.1"
                     /translation="SLLGLAFFAPAESPARFHESFDVELCVAPCRGARWALAQQVEGE
                     LETVSVGGRRALRAVTGPRRERVPKAALVARPPKVGPGGSIQVGFDMMIPEGAPINSI
                     HLVDIECATCGVGGNPGIRLYLRNGRLRIDRAKIGVRHAWTADEAPQLQPGRWHRIEW
                     SVLLGAESGAALVRLDGRTVLEGQGATIIPPAGGAAPGADRVQIGLTASSNTVPATAY
                     FDNVTVEAGR"
     gene            complement(712..1539)
                     /locus_tag="VF589_09930"
     CDS             complement(712..1539)
                     /locus_tag="VF589_09930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8257942.1"
                     /translation="MHLNVAAATGSPHVSDLSELQNSPSAIGAETTFNDLSGYVIVTP
                     EEIRAPDMAARVAAECRECDVPLRLGEDRDFYFAVAKPMFDRVAAALLLVLLAPVFLA
                     IAAMIKLSDGGPVFFQQPRTGHMARRFRLLKFRTMIPGAEHERARIWHMNIHGDGTPD
                     FKAKDDPRVTPLGRHLRSTSLDELPNLINVALGQMSLVGPRPTSFDASTYAPCHLARL
                     AVKPGITGLWQVSGRASIDFNRRCELDKHYIRTASAATDAALLLKTVSAVIRRNGAH"
     gene            complement(1747..>2185)
                     /locus_tag="VF589_09935"
     CDS             complement(1747..>2185)
                     /locus_tag="VF589_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257943.1"
                     /translation="GALAAPKFADGARETDAQFRARIAADLVRCREAYAARLGHPPTL
                     FCWPENRVAGAGREVAEKLGYRATTAGNGRNTAAEPPSILSRIHVGDRALGFRWGPAE
                     ALRLRATVRLFQGNHYWYLVAAPMDLIRRIVFAARDRAGARYA"
BASE COUNT          346 a          723 c          772 g          344 t
ORIGIN      
        1 agcctgctcg gcctcgcctt tttcgctccc gccgaatcgc ccgcgcgctt ccacgagagc
       61 ttcgacgtgg agctctgcgt cgcgccctgc cgcggcgccc gctgggcgct ggcgcaacag
      121 gtggagggag agctcgagac ggtatcggtc ggcggccgcc gcgcgctgcg cgccgtgacc
      181 ggcccccggc gcgagcgggt gcccaaggcg gcgctggtcg cgcgcccgcc caaggtcggt
      241 cccggcggat cgattcaggt cggcttcgat atgatgattc cggaaggggc gccgatcaat
      301 tcgatccatc ttgtcgacat cgagtgcgcg acctgcggcg tcggcggcaa cccgggcatc
      361 cggctctacc tccgcaacgg ccggctccgg atcgaccgcg cgaagatcgg cgtccgccac
      421 gcctggaccg ccgacgaagc gccgcagctc cagcccggcc gctggcaccg gatcgagtgg
      481 agcgtgctgc tcggcgcgga aagcggcgcg gcgcttgtcc gcctcgacgg caggacggtg
      541 ctggaggggc agggggctac gatcataccg cctgcgggcg gcgccgcgcc cggggccgac
      601 cgcgtccaga tcggccttac cgccagctcg aacaccgtgc cggcaaccgc ttatttcgac
      661 aatgtgaccg ttgaggcggg gcggtagctc aacaccgacg cgagcgccgg ctcaatgggc
      721 tccattgcgg cggatcaccg cgctcaccgt cttcaggagc agggcggcgt cggtcgcggc
      781 cgacgcggtg cggatataat gtttgtccag ttcgcagcgc cgattgaagt cgatcgaggc
      841 gcggcccgag acctgccaca gtccggttat gccgggcttc acggcgagcc gcgccaagtg
      901 gcagggcgca taggtcgagg cgtcgaacga ggtggggcgc gggccgacca gcgacatctg
      961 gcctagcgca acgttgatca ggttcggaag ctcgtcgagg ctggtcgagc gcaggtgacg
     1021 accgagcggc gtcacccgcg gatcgtcctt cgccttgaaa tccggcgtgc cgtcgccgtg
     1081 gatgttcatg tgccagatcc gcgcccgctc atgctcggcg ccggggatca tggtccggaa
     1141 tttcagcaat cggaaacgcc gcgccatatg gccggtgcgc ggctgttgga agaagaccgg
     1201 accgccatcc gacagcttga tcatcgcggc gatggcgagg aacaccggcg ccagcagcac
     1261 cagcagcaaa gcggcggcga cgcggtcgaa catcggcttg gccacggcga agtagaaatc
     1321 cctgtcctcg cccagccgga gcggcacgtc gcactcccgg cattccgcgg cgacccgcgc
     1381 cgccatgtcg ggagcccgga tttcctccgg cgtgacgatg acgtagccgg aaaggtcgtt
     1441 gaaggtggtt tcggctccga tcgccgacgg cgaattctga agttccgaaa ggtccgagac
     1501 atggggagag cccgttgccg ccgcgacgtt cagatgcatg gtgataagcc ccagcaatac
     1561 aggaaggcag gattgcccgc tcgcttcgtt aaccttttct taagcctaca gtttcagcct
     1621 aggttaggga atatcgcctt tttcgcgccc gcggcactgc ctcccaatgt tttcaataag
     1681 ttgggtcgat ttcaggggct gctttgtccc accgtgggac acgcggtacc gtgattcccg
     1741 ccggtttcag gcgtagcggg cgccggcccg gtcgcgggcg gcgaagacga tccggcggat
     1801 gagatccatc ggcgccgcga cgaggtacca ataatggttg ccttggaaga ggcgcacggt
     1861 ggcgcggagc cgcagcgcct cggcgggtcc ccagcggaag cccaaagcgc ggtcgccaac
     1921 atggatgcgc gacaggatcg agggcggctc ggcggcggtg ttgcggccgt tccccgcggt
     1981 ggtggcgcga tagccgagct tttcggcgac ctcgcggccg gcaccggcca cgcggttctc
     2041 cggccagcag aaaagggtag ggggatggcc gagccttgcc gcataggctt cgcggcagcg
     2101 gacgagatcg gcggcgatcc gcgcccggaa ctgcgcgtcc gtctcgcgcg cgccgtccgc
     2161 gaacttcggc gccgccagcg cgcct
//
LOCUS       DASVRK010000252        12032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_239324, whole genome shotgun sequence.
ACCESSION   DASVRK010000252 DASVRK010000000
VERSION     DASVRK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239324"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VF589_09940"
     CDS             complement(<1..668)
                     /locus_tag="VF589_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8257944.1"
                     /translation="MGDENDNVIDSGYLGDMIFAMGGNDIVNGGWGDDSVFGGIGNDK
                     LNGGQNNDTLYGEAGDDTINGDTGHDILNGGAGKDVINGGAGDDIFMVTSAAELHGIL
                     NGKAWGDVYDGGSGVDMLSLFFTSSTTAINFSGKDPRLANTFGAASITGFEQYQIMGG
                     TAADTITGYLLSDFLYGGKGDDTLSGLDGFDFLQGGDNNDTLHGGNAGDALMGDAGND
                     IVNGG"
     gene            complement(980..1792)
                     /locus_tag="VF589_09945"
     CDS             complement(980..1792)
                     /locus_tag="VF589_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8257945.1"
                     /translation="MAPPTEPGRIRVGIGGWTYEPWRGAFYPPGLPKSRELDFAVAHV
                     TAIEINATYYGRQKPESFARWAEAAPPGFVYAVKASRFCTNRKILTEAEQSIGIFCNQ
                     GFTQLGDKLGPILWQFAPTKQFDPDEFAAFLKLLPASQDGIALRHALEVRHESFRDPR
                     FVALARDAGAAIVFADSADYPQIADLTAGFVYARLQQAREEEPLGYDAPALDRWTEAA
                     RAWAAGDAPAGAAYVSPEPPPKVPRDVFMFMINGAKVRAPAAAQAVIAKLGG"
     gene            complement(1811..2548)
                     /locus_tag="VF589_09950"
     CDS             complement(1811..2548)
                     /locus_tag="VF589_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX8257946.1"
                     /translation="MQAPDIVSREAWLEARRKLLDREKAHSRAHDRLMEERRALPWVR
                     IDKEYVFETEQGPRTLADLFEGRSQLIVYHFMYPPGAAEGCGGCSFISDHIDGANLHL
                     AHHDVTLLAVSRAPLADFLPFKRRMGWRFDWVSSAGSDFNYDFGASFTPEQVAGQELL
                     YNFGTLPAFMEDLHGESVFYRNEAGEIFHTYSSYARAGDILIGAHNFLDLTPKGRNEG
                     SIMDWVRLHDRYEQPAAACCHAAEAAE"
     gene            complement(2607..4352)
                     /locus_tag="VF589_09955"
     CDS             complement(2607..4352)
                     /locus_tag="VF589_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX8257947.1"
                     /translation="MSDALLSSAILLLALGLFVSDRIRPDAVALIVLLIAWLTGLLSM
                     DEALDGFSSTAVLTVAAVLVVGRAIEFTGAAATITRFMVPRVPFVTVRLAGVLAMGVL
                     LSAFMNNIAALVITMPAALAIAREHKLPPGAVLMPLSFATILGGMTTLIGTPANLIIS
                     SVREDELGAPFGMFSMTPVAGAVAVAGIVYLIAIGWRLTPRRPTAPDAPDKIPVLVFE
                     LGLPIGAIQGRTTVATARQALRGAGAVLLAVLRDGQRARLDADAILHHDDRLLVMSRQ
                     DPWAVAARARLIALDPPGEGEGLVTARVSVVHGSSLEGESYGAIEARTEGAVRVIAAG
                     PRAAREKRPLASMIIGAGDQLFLHGRADEVQTLIRRGRLLEISRQAPPVRPGRAAMLV
                     MLIYGAALFLSTVFGISTTAAFIAAALAICMLRLLPAEEAYRSIDLPVIVLIAALIPI
                     GRAFDEAGGTTAIAGAIGWALSGAPLFLMLAVICALSMLLSIFLNNVATALVMAQVGL
                     GAAATLGIDPDAALLAVLIGSSCDFLTPIGHHNNLLVMRPGGYLFRDYPRVGAPLSVI
                     VILITAYVLSRTYGA"
     gene            4549..5877
                     /locus_tag="VF589_09960"
     CDS             4549..5877
                     /locus_tag="VF589_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8257948.1"
                     /translation="MRQAGKPFWRTLYFQVLVAIALGILTGWLFPAFGSALKPLGDGF
                     IKLVKMIITPVIFLTVVTGIAGMADLKAFGRVGAKAMAYFLTFSTLALAVGLVVANVA
                     RPGAGMNIDPATLDQGAVASYVTKAKEQSITDFVLHIIPDTAISAFTSGEILQVLFVA
                     ILFGVSLALIGDKGERLLGSLRTLTAIVFRMVHILMYAAPIGAFGAMAFTIGEYGIGT
                     LANLAGLIVTFYVTSLLFVVVVLGLVARVAGFSLLGLVRYIKDELLLVLGTSSSESAM
                     PLLMEKLERAGCPKPIVGLVVPTGYSFNLDGTNIYMSLAALFIAQATNTPLTLGDQLL
                     LMAVAILSSKGAAGVTGSGFIVLAATLSVVPAVPVAGMALILGVDRFMSECRALTNFT
                     GNAVATIVVSRWERAVDLDRLRAAFAGGGGELTEAESDALQIEERSDVPA"
     gene            5874..6299
                     /locus_tag="VF589_09965"
     CDS             5874..6299
                     /locus_tag="VF589_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEX8257949.1"
                     /translation="MSEFDLERFVAAQAPVYAQALAELEDGRKLSHWMWFVFPQLAGL
                     GRSENARFYGIGSAAEARAYLAHPVLGARLGACTAAVLRHRGTSANMIFGSVDAMKFR
                     SSMTLFEAVAETPEPFASALETFYEGDRDEATLELLGSG"
     gene            6548..8104
                     /locus_tag="VF589_09970"
     CDS             6548..8104
                     /locus_tag="VF589_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257950.1"
                     /translation="MRRQKRVLMAVAVLAAGLGSAPAQAELLPRPDEIALPGEKALLT
                     EIVSLLTEPSTDVAEGMAALDKALARLGQPTRLRGFVQFMRAQALFHQERGAPAREAV
                     EESIRLLPEYSAPLLLGTGIYVYSDQPGRAADYLMRASRIDPAVVAKMPEYEVDNLIH
                     RLTAQHDQRRVRLVSERLLEIGWLSQSLSSRSKLALRAIEARMAEGDAAGARKLVPKL
                     LVPSDSIELLIQSRYRELWPDIERWAGAELRDQWRVYLSETRAQWDASKDMASALSYA
                     RALDAAGHYDTLIRELLPLFSKPLDKIEDYDLLYVAGLVAGALARKSRWDEIETMYQR
                     AGKVWVMGEHANALNLAANRARYLFFRGKTQEGLTLMDAVIADAKRWGGEINSNALAA
                     MHRYRACMLHALGRAEESVLSRARGISGEGPGSTAELYVCLDKPEGARDMLIAALDDP
                     GKRAEVLNYMQKNDDVPMMSDYGRQSRARWEALRADPKLLAAIARHGRVLPYTYRAGA
                     PAELPPAKPL"
     gene            8350..8868
                     /locus_tag="VF589_09975"
     CDS             8350..8868
                     /locus_tag="VF589_09975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8257951.1"
                     /translation="MRLETEAGPILVEVDGKRAPVTAANFLRYVDEGRLDRTSFYRAA
                     QTKGAAKGEARGFIQGGIRRNYRKMLPPIAHEPTSETGLSHVAGAISMARSETGAGAM
                     GEFFIAASAMPSMDAKDGEPGYAVFGKVVEGMDTVRRILAAPTTPNAGRGAMRGQMIA
                     KPVAIVTARRAE"
     gene            complement(8890..9717)
                     /locus_tag="VF589_09980"
     CDS             complement(8890..9717)
                     /locus_tag="VF589_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX8257952.1"
                     /translation="MKISRIAAFSFGERGGNPAGVALCDALPAAETMQRIAAEIGYSE
                     TAFAAPEDGKWRVRYFAPEAEVDFCGHATIALGAALALDRGGGRFPLLINKGEISVEA
                     AGEGAAITAALQSPPTSSRPAPDALVAAALALFEFAPEDLDPRIPPAIANAGVDHLIL
                     ALRTRAALAAMRYELDAGRALMNAAGLVTISLVHAESERRFHARNPFASGGVYEDPAT
                     GAAAAALAGYLRDLAWPHGGAIEIIQGEDMNVPSRLLAELGSEPGSSIRVSGTARLM"
     gene            complement(9750..10346)
                     /locus_tag="VF589_09985"
     CDS             complement(9750..10346)
                     /locus_tag="VF589_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257953.1"
                     /translation="MIKPIFLAGALSLALSGCGGTAAGDAQANAAAATPAAAAGDDHG
                     EEGGSPSLLLAADGIMPGGTEESMVKFGANSLDTIEKVTPLVGETYQKEESGECGAGP
                     MEFAKWENVVLNFQNGEFVGWSLQAASDKPFIGTPGGLTIGSPRSDLDAALPKPLTVE
                     QTSLGTEFNAGGFSGLLSDATPAAKVTALWAGTNCAMR"
     gene            complement(10452..10967)
                     /locus_tag="VF589_09990"
     CDS             complement(10452..10967)
                     /locus_tag="VF589_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8257954.1"
                     /translation="MGIFTSDIKSFDDLFVSVLQQIYYAERKIAEQLEVMIGKATSPE
                     LRAGFEQHLGETQNQYKRAEEAFRLFGREPEEATCPAIDGILKAGNSLAGEIDDKEVL
                     DAGLTHAAQLVEHYEIAQYGTLIAWAKEMGRQEIASLLQQTLDEEYATDKKLTGLAEA
                     RINRKAELQTA"
     gene            complement(11117..11929)
                     /locus_tag="VF589_09995"
     CDS             complement(11117..11929)
                     /locus_tag="VF589_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8257955.1"
                     /translation="MNLNKALWEKGDFTRIAESMRESGEALVDAIGVRPGMAMLDLGC
                     GDGTTALPAARRGADVLGVDIAANLVAAGNARAAAAGLGNLRFQEGDASDLAGIADAS
                     FDLVLSMFGAMFAPRPYDVAREMVRVTRSGGRIVMGNWIPGDPTLVAQILKISAAYTP
                     PPPEGFVSPVTWGVEDQVRDRFGAAGVAPENIACERATWVFRTPRPPSEQLNIFRTYY
                     GPTMNAYEAAARDGREQQLHAELTDLFETQNRARDGGTEIAATFLKVTVSKE"
BASE COUNT         1836 a         3892 c         4417 g         1887 t
ORIGIN      
        1 ccgccgttga cgatgtcgtt gccggcatcg cccatcagcg cgtcgccagc gttgccgccg
       61 tggagggtgt cgttattgtc cccaccctgg aggaagtcga agccgtcgag gccgctgagc
      121 gtgtcgtcgc ccttgccgcc atagaggaag tcgctgagca ggtagccggt gatcgtgtcg
      181 gcggcggtgc cgcccatgat ctgatattgc tcgaagccgg tgatgctcgc cgcgccgaaa
      241 gtgttggcga ggcgcgggtc cttgccgctg aagttgatcg cggtggtgct ggaggtgaag
      301 aacaggctca gcatgtcgac gccgctgccg ccgtcataga cgtcgcccca ggccttgccg
      361 ttgaggattc cgtgcagctc ggccgcgctg gtcaccatga agatgtcgtc gccggcgccg
      421 ccgttgatca cgtccttgcc ggcgccgccg ttcaggatgt cgtggccggt gtcgccgttg
      481 atcgtgtcgt cgccggcttc gccgtagagc gtgtcgttat tctggccgcc gttgagcttg
      541 tcgttgccga tgccgccgaa tacggaatcg tcgccccagc cgccattgac gatgtcgttg
      601 ccgcccatgg cgaagatcat gtcgcccaaa tagccgctgt cgatgacgtt gtcgttctcg
      661 tcacccataa tcggatccgc cactttacta tccccttgaa actcgtgagt tgcgatcttg
      721 ccgccgccgg ggaattagcc ggcggcgatt ccggcgcgag aaccggtcga cgaagccgga
      781 gcgggattcg accaacgccg cgccggtgcg acgaacatga ccttccgtcc gaccaaagcc
      841 gcaggcggag ccgccggggg tctcatgaac gccgctcagc gcgttggttt ggaagcagga
      901 ttcccgggct cctcgtcagc cgacgaacag gaagtccgtg agggcgaggc tggcgacgtg
      961 gtcgagcctc caggcgggtt cagcctccca gcttggcgat caccgcttgg gcggcggccg
     1021 gcgctctgac cttcgcgccg ttgatcatga acatgaagac gtcgcgcggg accttgggcg
     1081 gcggctcggg cgagacatag gccgctcctg ccggagcatc gcctgccgcc caggcgcggg
     1141 cggcttcggt ccagcgatcg agcgcggggg cgtcgtagcc gagcggctcc tcctcgcggg
     1201 cctgctgcag gcgggcatag acgaagcccg ctgtgaggtc ggcgatctgg ggatagtcgg
     1261 cggagtcggc gaagacgatg gcggcgccgg cgtcgcgcgc cagggcgacg aagcgcgggt
     1321 cgcggaagct ttcgtggcgg acctcgagcg cgtggcggag ggcgatgccg tcctggctgg
     1381 ccggcaggag cttgaggaaa gcggcgaact cgtcagggtc gaattgcttg gtgggggcga
     1441 attgccagag gatcgggccg agcttgtcgc cgagctgggt gaagccctgg ttgcagaata
     1501 tgccgatcga ttgctcggcc tcggttagta tcttgcggtt ggtgcagaag cgcgaggcct
     1561 tgactgcgta gacgaaaccg ggcggcgcgg cctcggccca gcgggcgaag ctttccggct
     1621 tctggcggcc gtaataggtg gcgttgatct cgatcgcggt gacgtgggcg acggcaaaat
     1681 cgagctcgcg cgatttcggc aggccgggcg ggtagaaggc gccgcgccac ggctcgtaag
     1741 tccagccgcc gatgcccacc cggatccggc ctggttcagt aggcggcgcc accgttcggc
     1801 ctcctccccg tcattccgcc gcctcggccg cgtggcagca ggcggccgcc ggctgctcgt
     1861 agcggtcgtg gaggcgcacc caatccataa tgctgccttc gttgcggccc ttgggcgtca
     1921 gatcgaggaa attgtgggcg ccgatcaata tgtcgccggc gcgggcgtag ctggaataag
     1981 tgtggaagat ctcgccggcc tcgttccggt agaagacgct ctcgccgtga aggtcttcca
     2041 tgaaggccgg caaagtgccg aaattgtaga gcagctcctg ccccgccacc tgctccggcg
     2101 tgaaggaggc gccgaaatca tagttgaagt cgctgcccgc cgacgagacc cagtcgaagc
     2161 gccagcccat gcgccgcttg aacggcagga aatcggcgag cggcgcgcgc gagaccgcga
     2221 gcagggtgac gtcgtgatgg gcgagatgga ggttggcgcc gtcgatatgg tcggagatga
     2281 aggagcagcc gccgcagcct tccgccgcgc cgggcgggta catgaaatga tagacgatga
     2341 gctggctgcg cccctcgaac agatccgcga gcgtgcgcgg gccctgctcg gtctcgaaga
     2401 catattcctt gtcgatcctg acccagggaa gcgcgcggcg ctcctccatc aggcggtcgt
     2461 gcgcgcggct gtgcgccttt tcgcggtcga gcagcttgcg acgcgcctcc agccatgcct
     2521 cgcgcgatac gatgtccggt gcctgcatgt tcgcctccca tgagctggcg cggaaggatt
     2581 gagacagctc gccccgcgcg gccgagtcaa gcgccatagg tccggctcag gacataggcg
     2641 gtgatgagga tgacgatcac cgacagcggc gccccgacgc gcggatagtc gcggaacaga
     2701 tagccgccgg ggcgcatcac cagcagattg ttatggtggc cgatcggcgt caggaaatcg
     2761 caggaggagc cgatcaggac cgcgagcaag gccgcgtcgg gatcgatgcc gagcgtggcg
     2821 gcggcgccga gcccgacctg agccatgacc aaagcggtgg cgacgttgtt gaggaagatg
     2881 gagagcagca tcgacagcgc gcagatcacc gccagcatca ggaacaaggg cgcgcctgag
     2941 agcgcccagc cgatcgcgcc ggcgatggcg gtggtgccgc cggcctcgtc gaacgcgcgg
     3001 ccgatcggga tcagggcggc gatgaggacg atcaccggca ggtcgatcga gcgataagcc
     3061 tcctcggccg ggagcaggcg gagcatgcag atggcaagcg cggcggcgat gaaggcggcg
     3121 gtggtcgaga tcccgaagac ggtcgagagg aacaaagccg cgccgtagat tagcatgacc
     3181 agcatcgccg cgcggccggg gcggaccggc ggcgcctggc ggctgatctc gagcagccgc
     3241 ccccgccgga tcaaagtctg cacctcgtcg gcgcggccgt ggaggaaaag ctggtcgccg
     3301 gcgccgatga tcatcgaggc gagcggccgc ttctcgcggg ccgcgcgcgg gccggccgcg
     3361 atcacccgga cggcgccttc ggtgcgcgcc tcgatcgcgc cgtagctctc cccttcgagc
     3421 gaggagccgt ggacgaccga gacgcgggcg gtgaccagcc cctccccttc gccgggcgga
     3481 tcgagcgcga tcagccgcgc gcgggcggcg accgcccagg ggtcctgccg gctcatcacc
     3541 agcagccggt cgtcatggtg gaggatggcg tcggcgtcga gccgcgcccg ctggccatcg
     3601 cgaagcaccg cgagcagcac cgcgccggcg ccgcgcagcg cctggcgcgc ggtggcgacg
     3661 gtggtgcggc cctggatggc gccgatcgga aggccgagtt cgaacaccag caccgggatc
     3721 ttgtccggcg cgtccggggc ggtcgggcgg cgcggcgtca gccgccagcc gatcgcgatc
     3781 agatagacga tccccgccac cgccaccgcg ccggccacgg gcgtcatgct gaacatgccg
     3841 aacggcgcgc cgagctcgtc ctcgcggacc gaggagatga tgaggttggc gggcgtgccg
     3901 atgagggtgg tcatgccgcc gaggatggtg gcgaaggaga gcggcatcag aaccgcgccg
     3961 ggcggcagct tatgctcgcg cgcgatcgcc aatgcggccg gcatggtgat gaccaaagcc
     4021 gcgatattgt tcatgaaggc ggagagcagc acccccatcg ccagcacgcc cgccaggcgc
     4081 acggtgacga acggcacgcg cggcaccatg aagcgggtga tggtggccgc ggccccggtg
     4141 aactcgatcg cccggccgac caccagcacc gcggcgacgg tgagcaccgc ggtcgaggag
     4201 aagccgtcca gcgcctcgtc catgctgagc aggccggtga gccaggcgat gaggaggacg
     4261 atgagggcga ccgcgtcggg gcggatgcga tcgctgacga acaggcccaa tgccagcagc
     4321 aggatcgccg agctgaggag ggcatcgctc acggaagctt ctctgtcaca gcgggcggcg
     4381 ggctgccaag ccctgggtgc tccgcggtgg gagccgggtt ccaggccgga ctgcttctgg
     4441 gccccggctt tcgccgggga acggccgcgg ggaacgattg tgtccgagcg gcgctggcgc
     4501 cggatactga agccgggtta aggccaaggc cggcaacggg gacatggaat gcggcaagca
     4561 ggcaagccgt tctggcggac gctttatttc caggtgctgg tcgccatcgc gctcggcatc
     4621 ctcaccggct ggctgttccc ggcgttcgga tcggcgctga agccgctcgg cgacggcttc
     4681 atcaagctcg tcaagatgat catcacgccg gtcatcttcc tcaccgtcgt caccggcatt
     4741 gccggcatgg ccgacctcaa ggcgttcggc cgggtcggcg ccaaggcgat ggcctatttc
     4801 ctgaccttct cgacgctcgc gctcgcggtc ggtctggtcg tcgccaacgt cgcccgcccg
     4861 ggcgcgggca tgaacatcga cccggcgacg ctcgaccagg gcgcggtggc gagctacgtt
     4921 accaaggcca aggagcagtc gatcaccgac ttcgtgctcc acatcatccc cgacaccgcg
     4981 atcagcgcct tcacctcggg cgagatattg caggtgctgt tcgtcgccat cctgttcggc
     5041 gtctcgctcg ccctgatcgg cgacaagggc gagcgcctgc tcggctcctt gcgcacgctg
     5101 accgcgatcg tgttccggat ggtgcacatc ctgatgtacg ccgcgccgat cggcgcgttc
     5161 ggggcgatgg ccttcacgat cggggaatat ggcatcggca ccctcgccaa cctggctggg
     5221 ctgatcgtca ctttctacgt cacctcgctg ctgttcgtgg tggtcgtgct cgggctcgtc
     5281 gcccgggtcg cgggcttctc gctgctcggc ctggtgcgct acatcaagga cgagctgctg
     5341 ctggtgctcg gcacctcctc gtccgaaagc gcgatgccgc tgctgatgga gaagctcgag
     5401 cgggccggct gcccgaagcc gatcgtcggc ctggtcgtgc cgaccggcta ttcgttcaac
     5461 ctcgacggca ccaacatcta catgtcgctg gccgccttgt tcatcgccca ggcgaccaac
     5521 acgccgctga cgctgggcga ccagttgctg ctgatggcgg tggcgatatt gagctccaag
     5581 ggcgccgcgg gcgtgaccgg atcgggcttc atcgtgctgg cggccaccct gtcggtggtg
     5641 ccggcggtgc cggtggcggg catggctttg atcctcggcg tcgaccggtt catgagcgag
     5701 tgccgcgcgc tcaccaactt caccggcaat gcggtggcga cgatcgtggt ctcgcgctgg
     5761 gagcgggcgg tggacctcga ccggctgcgc gccgccttcg ccggcggcgg cggcgagctc
     5821 accgaagccg agagcgacgc gctccagatc gaggagcgga gcgacgtgcc ggcatgagcg
     5881 aattcgacct cgagcggttc gtcgcggcgc aggcgccggt ctacgcgcag gcgctggccg
     5941 agctcgagga cggccgcaag ctgagccact ggatgtggtt cgtcttcccg cagctggccg
     6001 ggctcgggcg gagcgagaat gcgcgtttct acggcatcgg ctcggccgcg gaggcgcgcg
     6061 cctatctcgc ccatcccgtg ctcggggccc gccttggcgc atgcaccgcg gccgtgctgc
     6121 gccaccgcgg gaccagcgcg aacatgatct tcgggtcggt cgacgcgatg aagttccggt
     6181 cgtcgatgac gctgttcgag gcggtggcgg agacgccgga gccgttcgcc agcgcgctcg
     6241 aaaccttcta cgagggcgac cgggacgagg cgacgctcga gctgctgggg agcggctgac
     6301 gcggcaaaga cggggaggat ttcgggcagg cgatacccgg agccgtaaga ggtgatccgt
     6361 cattccagcg gaagctggaa tctcgcttct tcttcttctc gggctcccaa gggaaagtga
     6421 gatgccagct ttcgctggca tggcggttga ttgcatatcc aatcagctcc caccgtttac
     6481 ctccgcctcg gccggctttg acttgggccg cgacgtagct aacctgttgc cgcagcggga
     6541 gaagagaatg cggcggcaaa agcgggttct gatggcggtc gccgtgctgg cggcggggct
     6601 tgggtcggcg ccggcgcagg cggagctgct gccgcggccg gacgagatcg cgctgccggg
     6661 cgagaaggcg ctgctcaccg agatcgtgtc gctgctgacc gagccgagca ccgatgtggc
     6721 ggaggggatg gcggcgctcg acaaggcgct cgcgcgtctc ggccagccga cccggctgcg
     6781 cggcttcgtg cagttcatgc gcgcccaggc cctgttccat caggaacgcg gcgccccggc
     6841 gcgcgaggcg gtcgaggaga gcatccgcct gttgccggaa tatagcgcgc cgctgctgct
     6901 cggcaccggc atctacgtct atagcgacca gcccggacgg gcggcggact atctgatgcg
     6961 cgcgagccgg atcgatcccg ccgtggtcgc caagatgccg gaatatgaag tcgacaattt
     7021 gatccaccgc ctgaccgccc agcacgacca gcgccgggtc cggctggtca gcgagcggct
     7081 gctcgagatc ggctggctgt cccagagcct ctcgagccgc tcaaagctcg ccctgcgcgc
     7141 catcgaagcg cggatggcgg aaggcgacgc cgcgggcgcg agaaaattgg tgccgaagct
     7201 gctggtgccg tcggattcga tcgagctgct gatacagagc cggtatcggg agctgtggcc
     7261 ggacatcgag cgctgggcgg gcgccgagct tcgcgaccag tggcgggtct atctcagcga
     7321 gacgcgcgcg caatgggacg ccagcaagga catggcgagc gcgctcagct atgcgcgggc
     7381 gctggacgcg gccggccatt acgacacatt gatccgggag ctgctgccgc tcttctccaa
     7441 gccgctcgac aagatcgaag attacgacct gctctatgtc gccggactgg tcgcgggggc
     7501 gctggctcgg aaaagccggt gggatgagat cgaaaccatg taccagcgcg ccggcaaggt
     7561 atgggtcatg ggcgagcatg ccaatgcgct gaacctggcg gcgaaccggg cccgctacct
     7621 gttcttccgc ggcaagacgc aggagggtct cacgctgatg gacgcggtga tcgcggacgc
     7681 gaagcgatgg ggcggcgaaa tcaatagcaa tgcccttgcc gccatgcacc gttaccgggc
     7741 ctgcatgctc catgccctgg gaagggccga ggaatcggtg ctgtcccgcg cccgcggcat
     7801 cagcggcgag gggccgggct ccacggccga gctctacgtg tgcctcgaca agccggaagg
     7861 ggcgcgggac atgctgatcg ccgcgctcga cgatccgggc aagcgcgcgg aggtgctgaa
     7921 ttatatgcag aagaatgacg atgtgccgat gatgagcgac tatggccggc aatcgagggc
     7981 gcggtgggag gcgctgcggg cggaccccaa gctgctcgcc gcgatcgccc gccacgggcg
     8041 ggtcctgccc tatacgtacc gcgccggcgc gccggcggag ctgccgccgg ccaagccgct
     8101 ttagccgaag agcggcccag ccgcggcggc ggcccgatca atgggggtgc gcccccgccc
     8161 cgatcctgtc agcttaagcg tgacatgatt cgctttccct gtctggcctt gatggccctg
     8221 ctcgcggcgg gttgcggcga tggcggcacc gcggaggcac cggccaacgg ctcggctgcc
     8281 gccgggtccg ccgcggcacc cccctccccc gctccgtccc cgcccgccct tgtccccgac
     8341 aaggcccggg tgcggctcga gacggaagcg gggccgatcc tggtcgaggt cgacggcaag
     8401 agggcgccgg tcaccgccgc caatttcctg cgctacgtcg acgaggggcg gctcgaccgc
     8461 accagcttct atcgcgccgc gcagaccaag ggggcggcga agggcgaggc gcgcggcttc
     8521 atccagggcg ggatccggcg caattatcgc aagatgctgc cgccgatcgc gcacgagccg
     8581 accagcgaga ccgggctcag ccacgtcgcc ggcgcgatct cgatggcgcg cagcgaaacc
     8641 ggggcgggcg cgatgggcga attcttcatc gcggcgagcg cgatgccgtc gatggacgcc
     8701 aaggacggcg agccgggcta tgcggtgttc ggaaaggtgg tggaggggat ggacacggtc
     8761 cgccgcatcc tcgccgcgcc gaccacaccg aatgccgggc gcggcgcgat gcgcgggcag
     8821 atgatcgcca agccggtggc gatcgtgacg gcgcggcggg ccgagtgagg tcggccgcgg
     8881 cgccgaggat cacatcagcc gggcggtgcc ggagacgcgg atgctggagc cgggctcgga
     8941 gccgagctcc gcgaggagcc ggctggggac gttcatgtcc tcgccctgga taatctcgat
     9001 ggcgccgccg tgcggccagg cgaggtcgcg caggtagccg gcgagcgcgg ccgcggcggc
     9061 gccggtcgcg gggtcttcgt agacgccgcc cgacgcgaac gggttgcggg cgtggaagcg
     9121 gcgctcgctc tcggcatgga cgaggctgat cgtgaccaga ccggccgcgt tcatcagcgc
     9181 gcggccggcg tccagctcgt agcgcatcgc cgccaacgcg gcgcgggtcc ggagggcgag
     9241 gatcaggtga tcgacgccgg cattggcgat agcgggcgga atgcgcgggt cgaggtcttc
     9301 gggggcgaat tcgaacaagg ccagcgcggc ggcgacgagc gcgtccggcg cggggcggct
     9361 ggaggtcggc ggcgactgga gcgcggcggt gatggccgcg ccctccccgg ccgcctcgac
     9421 gctgatctcg cctttgttga tgagcaaggg aaagcggccg ccgccgcggt cgagcgccaa
     9481 agcggcgccc agcgcgatgg tggcgtggcc gcaaaagtcg acctcggctt cgggggcgaa
     9541 atagcggacc cgccacttgc cgtcctcggg cgcggcgaaa gcggtctcgg aatagccgat
     9601 ctcggcggcg atgcgctgca tggtctcggc cgcggggagc gcgtcgcaaa gcgcgacgcc
     9661 ggcggggttg ccgccgcgct cgccgaacga gaaagcggcg atcctggaga ttttcatggc
     9721 ccctcccgtc gcctcctgtg tcaggccgct cagcgcatcg cgcaattcgt gccggcccag
     9781 agcgcggtca ccttggcggc gggggtggcg tcgctgagca ggccggaaaa gccgccggcg
     9841 ttgaactcgg tgccgagcga ggtctgctcg accgtcagcg gcttgggcag agcggcgtcg
     9901 agatcggagc gcggcgaacc gatggtgagg ccgccgggcg tgccgatgaa cggcttgtcc
     9961 gacgccgcct gcagcgacca gccgacgaac tcgccattct ggaaattgag cacgacattt
    10021 tcccacttgg cgaactccat cgggcccgcc ccgcattcgc ccgattcctc cttctgatag
    10081 gtttcgccga cgagcggcgt gaccttctcg atcgtgtcga ggctgttggc gccgaacttg
    10141 accatgcttt cctcggtgcc gcccggcatg attccgtcgg cggcgagcag caggctgggg
    10201 ctgccgccct cttcgccatg atcgtcgccg gcggcggcgg ccggcgtggc ggcggccgcg
    10261 ttcgcctggg cgtcgccggc ggcggtgccg ccgcaaccgg agagcgcaag cgacagcgcg
    10321 cccgcgagaa agattggctt gatcatattt gtccccctgc ccagcagcgg aagctgcggt
    10381 gggatcgtca gggggtcaag cgatttgtcg ccggcccccg cggccgtgcg gccgcgggga
    10441 ctgaggtccg gtcaggcagt ttgcagctcg gccttgcggt tgatccgcgc ctcggcgagg
    10501 ccggtcagct tcttgtcggt cgcatattcc tcgtccagcg tctgctggag caggctggcg
    10561 atctcctggc gtcccatttc cttggcccag gcgatcagcg tgccatattg ggcgatctca
    10621 taatgctcga ccagctgggc ggcgtgggtg aggccggcgt cgagcacttc cttgtcgtcg
    10681 atctcgccgg cgaggctgtt gcccgccttg aggatgccgt cgatcgccgg gcaggtcgct
    10741 tcctcgggct cgcggccgaa caggcggaag gcctcttcgg cgcgcttata ctggttctgg
    10801 gtctcgccca gatgctgctc gaagccggcg cgaagctccg gcgaggtcgc cttgccgatc
    10861 atcacctcga gctgctcggc gatcttgcgc tcggcataat agatttgctg gaggacgctg
    10921 acgaacaggt cgtcgaagct tttgatgtcg ctggtgaaga tgcccatgga cgtttccttc
    10981 gagattgggg gtggggccgc ccgcgcgtgt cgggaccggt ccaaaggtca acgtgcggga
    11041 ggggacggcg ttgccaacga tgcgcagccg aaaggacatt gccccattca gccggcgcat
    11101 ggagcgcggg ccgcgctcac tccttcgaca ccgtgacctt gaggaaggtg gcggcgatct
    11161 cggtgccgcc gtcgcgcgcg cggttctgcg tctcgaacaa gtcggtgagc tcggcgtgca
    11221 gctgctgctc ccggccgtcc ctcgccgccg cctcataggc gttcatggtc gggccgtaat
    11281 aggtgcggaa gatgttgagc tgctccgagg gcgggcgggg cgtgcggaag acccaggtgg
    11341 cgcgctcgca ggcgatgttt tcaggcgcca cgccggcggc gccgaagcgg tcgcggacct
    11401 ggtcctcgac gccccaggtc accgggctga cgaagccttc gggcggcggc ggggtgtaag
    11461 cggcgctgat cttgaggatt tgcgcgacga gggtcggatc gccggggatc caattgccca
    11521 tcacgatgcg gccgccggac cgcgtcaccc gcaccatctc gcgggcgacg tcgtagggcc
    11581 ggggcgcgaa catggcgccg aacatgctga ggacgagatc gaagcttgcg tcggcgatgc
    11641 cggcgaggtc ggacgcgtcg ccctcctgga agcggaggtt gccgaggccg gccgcggcgg
    11701 cgcgggcgtt gccggcggcg acgaggttgg cggcgatgtc gacgccgagc acgtcggcgc
    11761 cgcggcgcgc ggccgggagc gcggtggtgc cgtcgccgca gccgagatcg agcatggcca
    11821 tgccggggcg gacgccgatc gcgtcaacca gcgcttcgcc gctttcccgc atggactccg
    11881 cgatccgggt gaaatcgccc ttttcccaaa gcgccttgtt gaggttcatc ggcctggctc
    11941 ctcttcgggg cgggcgccta gacgagggcg cgcggcggcg acagcggcgc gcgcccaacc
    12001 tgccccgtgg gacgacgaag ctgcaggctg cc
//
LOCUS       DASVRK010000253         6146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_113156, whole genome shotgun sequence.
ACCESSION   DASVRK010000253 DASVRK010000000
VERSION     DASVRK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113156"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF589_10000"
     CDS             <1..323
                     /locus_tag="VF589_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX8257956.1"
                     /translation="FQRGEWAGANQPVVALIPDDRVFIRFFVPEGSVAAYRTGRRIAF
                     RCDGCGAPRAATIAYVSPRPEFTPPIIYSREARDRLVFMVEARPEPGAALTPGLPVDV
                     EPAS"
     gene            320..1237
                     /locus_tag="VF589_10005"
     CDS             320..1237
                     /locus_tag="VF589_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8257957.1"
                     /translation="MTVAIEVEGLNKAFAGRRVVKDFSIRVEEGRITGFLGPNGSGKT
                     TTLRMLCGLLTPDSGRGTALGLDIVADAAAIKRQTGYMTQRFSLYEDLTVEENLLFSA
                     RIHGLDRRRERVDASLADLGLAERRRQLAGTLSGGWKQRLALASATLHEPRLLLLDEP
                     TAGVDPQARRTFWDEIHALAAGGLTVLVSTHYMDEAERCHDIAYIAYGELMARGTADE
                     VIAGARLFTWRGEGPGVDRLSAELLRQPAVEMAAPFGLALHVSGSDAAGLEAALAPWQ
                     RPPFRWQRVEPTLEDVFIHLMSGAKDNFQ"
     gene            1234..2382
                     /locus_tag="VF589_10010"
     CDS             1234..2382
                     /locus_tag="VF589_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8257958.1"
                     /translation="MTSARASNAFSFTRVFAVLAKEFIQLTRDRITYAMLLGVPVMQL
                     LLFGYAINTDPRQLPTAVLVQENSRLARSALAAIANTGYFEIVAQARTPAELDALVRK
                     GDVQFAITIPGDFTRRVIRRDRAQLLVEADASDPAATGGAVAALAALPAEALRHDLEG
                     AARPAAAAQPFEIIVHRRYNPENITAYNIVPGLLGVILSMTLVMMTALGVTREQERGT
                     MESLLATPVQPLEVMAGKLAPYVVVGVIQTVIVLVLARLLFDVPMAGGWGGLTLGVFL
                     FIIGSLSLGFLISTLARTQLQAMQLSFFYMLPSILLSGFMFPFRGMPDWTQALGTVIP
                     VTHFLRIVRGALLKGQSFADMGPSLLALAIFVCAVAALALARYRTTLD"
     gene            complement(2379..3497)
                     /locus_tag="VF589_10015"
     CDS             complement(2379..3497)
                     /locus_tag="VF589_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8257959.1"
                     /translation="MSTDIDILEQEARTEAAAAAAAPDPAKLEAFMGKMVGDMGAANG
                     GALVMLGERLGLYKALAAIGPASPSELAERTGTHERYVREWLSAQAAAGYVDYDGPSG
                     LFAMSPEQAMALADENSPVYVASAFQLTRSLYLDEPKIAEAFRSGAGVGWHEHSDCLF
                     CGVEQFFRTGYRAHLVQDWLPALDGAVERLKVAAKVADIGCGHGASTIILAQAFPDSD
                     FVGLDYHEASIERARQAAREAGDPANLRFEVATAKDSPGEGYDLVTVFDALHDMGDPV
                     GAARRVREMLKPDGLFMIVEPAAGDTLADNLNPVGRMYYAASTMICTPGSLAQEVGLG
                     LGAQAGPKRLGEVLAEAGFTRVRVAASTPFNLILEARP"
     gene            complement(3687..4946)
                     /gene="clpX"
                     /locus_tag="VF589_10020"
     CDS             complement(3687..4946)
                     /gene="clpX"
                     /locus_tag="VF589_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX8257960.1"
                     /translation="MTKLSGGDSKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     NDIIREETKSALVKTRDGVPTPGEICKVLDDYVIGQMHAKRVLSVAVHNHYKRLNHGQ
                     KGADVELAKSNILLVGPTGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQASDYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLMEGTT
                     ASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIIADRLQGKSIGFGAYVAAPE
                     ERRTGEVLRQGEPEDLLKFGLIPEFVGRLPVIATLEDLDTEALVKILKEPKNALIKQY
                     AKLFDMEDVKLSFTDEALVAIAKRAIERKTGARGLRSILENILLDTMFDLPSMDGVDE
                     VMIDKDVVEGRKEPVRVYAEKKGESAA"
     gene            complement(5063..5704)
                     /gene="clpP"
                     /locus_tag="VF589_10025"
     CDS             complement(5063..5704)
                     /gene="clpP"
                     /locus_tag="VF589_10025"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX8257961.1"
                     /translation="MDHQDILAGLVPIVIEQSNRGERSFDIYSRLLRERIVFVTGPVE
                     DHMATLITAQLLYLESENPKKDIFMYINSPGGVVTAGMAIHDTMNYIRPRVGTVCIGQ
                     AASMGAFLLGAGEPGMRVALTNARIMIHQPSGGAQGMASDIEIQAREILRIRHRMNEL
                     MAKHTGQPIEAIETAVERDKFFSAEEARAFGIVDEVFDKRPAASEEDAAKNAA"
BASE COUNT          922 a         2272 c         1854 g         1098 t
ORIGIN      
        1 tcttccagcg cggcgagtgg gccggcgcca accagccggt agtggccttg atccccgacg
       61 accgcgtctt catccgcttc ttcgtgcccg aggggtcggt cgccgcctac cgcaccggcc
      121 gccgcatcgc cttccgctgc gacggctgtg gagcgccccg cgccgccact atcgcctatg
      181 tcagcccccg ccccgaattc accccgccga tcatctacag ccgcgaggcg cgcgaccggc
      241 tggtgttcat ggtcgaggcc cgccccgagc ccggcgccgc gctgacgccc ggcctgccgg
      301 tcgacgtcga gccggcgtca tgaccgtcgc aatcgaggtc gaggggctca acaaggcgtt
      361 cgccggccgc cgcgtcgtca aggatttctc gatccgggtc gaagaaggcc gcatcaccgg
      421 cttcctcggc cccaacggct ccggcaagac caccaccttg cgcatgctgt gcggcctgct
      481 cacgcccgat tccggccgcg gcaccgcgct cgggctcgac atcgtcgccg acgccgccgc
      541 gatcaagcgc cagaccggct acatgaccca gcgtttctcg ctctacgaag acctcaccgt
      601 cgaggagaat ctgctcttct ccgcccgcat ccacggcctc gaccgccgcc gcgagcgggt
      661 cgatgcctcc ctcgccgacc tcggcctcgc cgagcgccgc cgccagctcg ccggcactct
      721 ctcgggcggc tggaagcagc ggctggcgct cgcctccgcc accctccacg agccgcgcct
      781 gctgctgctc gacgagccca ccgccggcgt cgacccgcag gcgcgccgca ccttctggga
      841 cgagatccac gccctcgccg ccggcggcct caccgtgctc gtctcgacgc attatatgga
      901 cgaggccgag cgctgccacg acatcgccta tatcgcttat ggcgagctga tggcgcgcgg
      961 caccgccgac gaggtgatcg ccggcgcgcg cctgttcacc tggcgcggcg agggccccgg
     1021 cgtcgaccgg ctctcggccg aactgctccg ccagccggcg gtcgagatgg ccgctccgtt
     1081 cgggctcgcg ctccacgtca gcggcagcga cgcggccggt ctcgaagccg cgctcgcccc
     1141 gtggcagcgg ccgcccttcc gctggcagcg ggtcgagccg acgctggagg acgtgttcat
     1201 ccacctgatg agcggcgcca aggacaattt ccagtgacgt cggcgcgcgc ctccaacgct
     1261 ttctcgttca cccgggtgtt cgcggtgctc gccaaggagt tcatccagct cacccgcgac
     1321 cgcatcacct atgcgatgct gctcggcgtt cccgtcatgc agctcctgct gttcggctat
     1381 gcgatcaaca ccgatccgcg ccagctcccc accgccgtct tggtgcagga gaattcgcgg
     1441 ctcgcccgct cggcgctcgc ggcgatcgcc aataccggct atttcgagat cgtcgcccag
     1501 gcccgcacgc ccgccgagct cgacgccctc gtccgcaagg gcgacgtcca gttcgccatc
     1561 accatcccgg gcgacttcac ccgccgcgtc atccgccgcg accgcgcgca attgctggtc
     1621 gaggccgacg cctccgatcc ggccgccacc ggcggcgccg tcgccgctct ggccgccctc
     1681 cccgccgaag cgctccgcca cgatctcgaa ggcgccgccc ggcctgccgc cgccgcgcag
     1741 ccgttcgaga tcatcgtcca ccgccgctac aatccggaga atatcaccgc ctacaatatc
     1801 gtgcccgggc tgctcggtgt gatcctgtcg atgaccttgg tgatgatgac cgccctcggc
     1861 gtcacccgcg agcaggagcg cggcaccatg gaaagcctgc tcgccacccc ggtccagccg
     1921 ctcgaagtga tggccggcaa gctcgccccc tatgtcgtcg tcggggtgat ccagaccgtg
     1981 atcgtgctcg tcctcgcccg cttgctgttc gacgtgccga tggcgggcgg ctggggcggc
     2041 ctcacgctgg gcgtgttcct gttcatcatc ggctcgcttt cgctgggctt cctcatctcc
     2101 accctcgcgc gcacccagct ccaggcgatg cagctctcct tcttctacat gctgccctcg
     2161 atcctgctgt cgggcttcat gttcccgttc cgcggcatgc ccgactggac gcaggcgctc
     2221 ggcaccgtca tcccggttac ccatttcctg cgcatcgtcc gcggcgcgct cctcaagggg
     2281 caaagcttcg ccgacatggg cccgagcctg ctcgccttgg cgatcttcgt ctgcgccgtc
     2341 gccgccctcg ccctcgcccg ctaccgcacg accttggatt aaggccgcgc ctccaggatc
     2401 aggttgaacg gcgtcgaggc ggccacccgc acccgcgtga agccggcctc ggccagcact
     2461 tcgccgagcc gcttcggccc cgcctgcgcg ccgagcccga gcccgacttc ctgcgccaag
     2521 ctccccggcg tgcagatcat ggtggaggcg gcataataca tccgcccgac cgggttgagg
     2581 ttgtcggcca gcgtatcgcc cgccgccggc tcgacgatca tgaacaggcc gtccggcttc
     2641 agcatctcgc gcacgcgccg cgccgcgccg accggatcgc ccatgtcgtg gagcgcgtcg
     2701 aatacggtga cgaggtcgta gccctcgccc ggactgtcct tggccgtcgc cacctcgaag
     2761 cgcaaattgg ccgggtcgcc ggcctcccgc gccgcttgcc ttgcgcgctc gatcgaggct
     2821 tcgtgataat cgagcccgac gaagtcgctg tccggaaagg cctgcgccag gatgatcgtc
     2881 gaggcgccgt ggccgcagcc gatatccgcc accttcgccg ccaccttcag ccgctcgacg
     2941 gccccgtcca gcgccggcag ccagtcctgg accaggtgcg cgcggtagcc ggtgcggaag
     3001 aattgctcga cgccgcagaa caggcaatcc gaatgttcgt gccagccgac cccggcgccg
     3061 ctgcggaacg cttcggcgat cttcggctcg tccagataga gcgaccgggt gagctggaac
     3121 gcgctcgcga cgtagaccgg cgaattctcg tcggcgagcg ccatcgcctg ctcgggcgac
     3181 atcgcgaaca gcccgcttgg cccatcatag tcgacataac ccgccgccgc ctgcgcgctc
     3241 agccattcgc gcacatagcg ctcatgggtg ccggtgcgtt cggcaagctc gcttgggctc
     3301 gccggcccga tcgccgccag cgccttgtac agcccgagcc gctcgccgag catcaccagc
     3361 gccccgccat tggcggcgcc catgtcgccg accatcttgc ccatgaaggc ttcgagcttg
     3421 gcggggtccg gcgcggccgc cgcggccgcc gcctccgtgc gagcttcctg ctcgagaatg
     3481 tcgatatcgg tgctcatgtt cgtggccttc cctggttgag acgcagtccc gctagcggcg
     3541 ggccgtgggc gccaccgtat gtgccagcgt tggaattgcg tccccaaggc cccagggccc
     3601 cgccccccgc acggcgcgac tctcccgctg ccgaagcagt cgaatcctat atcaggaaaa
     3661 gccggccgtg ggcagccgcc gcccggtcag gcggcgcttt cgcctttctt ctcggcatag
     3721 acgcggaccg gctccttgcg gccttcgacc acgtccttgt cgatcatcac ctcgtcgacc
     3781 ccgtccatcg aaggcaggtc gaacatggtg tcgagcaata tgttctcgag gatcgagcgc
     3841 agcccgcgcg cgccggtctt gcgctcgatc gcccgcttgg cgatcgccac cagggcctca
     3901 tcggtgaagg agagcttcac atcctccatg tcgaacaatt tggcatattg cttgatgagc
     3961 gcgttcttcg gctccttgag gatcttgacc aaagcctcgg tgtcgaggtc ctccagcgtc
     4021 gcgatcaccg gcaggcggcc gacgaattcg gggatcaggc cgaacttcaa cagatcctcg
     4081 ggctcaccct ggcgcagcac ctcgccggtg cggcgctcct cgggagccgc cacataggcg
     4141 ccgaagccga tcgacttgcc ctgcaggcgg tcggcgatga tcttctcgag gcccgcgaac
     4201 gcgccgccgc agatgaacaa tatgttggtc gtgtcgacct gcaggaattc ctgctgcgga
     4261 tgcttgcgcc cgccctgcgg ggggacggag gcggtggtgc cttccatcag cttcagcagg
     4321 gcctgctgca cgccttcgcc cgaaacgtcg cgggtgatcg acggattgtc cgacttgcgg
     4381 ctgatcttgt cgatctcgtc gatatagacg atcccgcgct gcgcccgctc gacattatag
     4441 tcggacgcct ggagcagctt caggatgatg ttctcgacat cctcgccgac atagccggcc
     4501 tcggtcaaag tcgtcgcgtc ggccatcgtg aacggcacgt cgaggattcg cgccagcgtc
     4561 tgggcgagca aggtcttgcc gcagccggtc ggcccgacca gcaggatgtt cgacttggcg
     4621 agctcgacgt ccgcgccctt ctggccgtgg ttcaagcgct tgtaatgatt gtgcaccgcc
     4681 accgacagca cccgcttggc gtgcatctgg ccgatcacat aatcgtccag caccttgcag
     4741 atctcgcccg gggtcgggac cccgtcgcgg gtcttcacca gcgcggactt ggtctcctcg
     4801 cggatgatgt cgttgcacag ctcgacgcac tcatcgcaga tgaacacggt cgggccggcg
     4861 atcagcttcc tcacctcatg ctgcgatttg ccgcagaagg agcagtaaag ggtgctcttc
     4921 gaatcgccgc cgctcaattt cgtcatgctt cgtccttcgg gcgcgaggcc cgcctgtcag
     4981 tgccgccatc ctgaccgctt cttaccacga tcacaatggt ataatcgtta agtccgctca
     5041 cgcggaccgt ccggcctaaa cgctaggccg cgtttttcgc cgcatcctct tccgacgccg
     5101 cgggccgttt gtcgaaaact tcgtcgacga tgccgaacgc cctggcctcc tcggcgctga
     5161 agaatttgtc gcgctcgacc gcggtctcga tcgcctcgat cggctggccg gtatgcttgg
     5221 ccatcaactc gttcatccgg tgccggatgc gcaatatctc ccgggcctgg atctcgatgt
     5281 ccgaggccat gccctgggcg ccgcccgacg gctggtggat catgattcgc gcgttggtca
     5341 gcgccacccg catgccgggc tcgccggcgc cgagcaggaa cgcgcccatc gaggcggcct
     5401 ggccgatgca gaccgtgccg acccgcggcc ggatgtagtt catcgtgtcg tggatcgcca
     5461 tgccggccgt caccaccccg cccggcgagt tgatgtacat gaagatatcc ttcttgggat
     5521 tctccgattc gaggtacagc agctgggccg tgatcagcgt cgccatatgg tcctcgaccg
     5581 gcccggtcac gaacacgatc cgctcccgca gcagccgcga atagatgtcg aagctgcgct
     5641 cgccgcgatt cgactgctcg atgacgatcg gcacgagtcc ggcgagaatg tcctgatgat
     5701 ccatgatgtg ctgggaatcc tagaagtggg agctgcccag gcaacatcgg acgttccggg
     5761 cggcggttca acccccgacc ggcccggcgg gcgcgatcac ccacgggagg accttcacga
     5821 ccgccggcgc gcaagcggcc gcttcgctcc cgaccggcac gcatccgacg tgccgtttcg
     5881 attcgtcgca tctccgtttt ctcggcgaac aaacagcaac atgcgcttgt aacttagatt
     5941 agtgcagtct tgtaacttag attagacaag caaagtacgt taccgtactc aacctgatcc
     6001 ccgccagctt tcccggaaca gttaaccaag actaagagtc tggaaaattg cggttcattg
     6061 ccttcgctcc gccatcgtta ctcggcatcc attcagggag ctcctccatg aaaatcatca
     6121 cgctcgtcac gacttcagcc ctcgcc
//
LOCUS       DASVRK010000254        24449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_295453, whole genome shotgun sequence.
ACCESSION   DASVRK010000254 DASVRK010000000
VERSION     DASVRK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 24449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24449
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295453"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VF589_10030"
     CDS             complement(<1..459)
                     /locus_tag="VF589_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8257962.1"
                     /translation="MYRPADFTQTLEGDTSVVRFTGGMTIAGIDGLPERLDQIHGERL
                     ILDLSNVERMDTVGAWLVHRLARDRDARLVGVGHDEAVLLEQVGQADQPVKMRPDRRP
                     PLYRVMDEVGEATALAAGTMLGLLGFFGELLVSAWNVATHPRRFRFNAVVQ"
     gene            547..1224
                     /locus_tag="VF589_10035"
     CDS             547..1224
                     /locus_tag="VF589_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEX8257963.1"
                     /translation="MKAALAIYDMDRTITRRATYTPFLIHAALRLAPWRLALVPVAVL
                     AALAYLARLIDRARLKEVNYRLLIGGSVEPSRLDPVIQSFAERQVAANIMPGARASIA
                     ADRAAGRRLVMATASYRIYAAAIAERLGFKDVIATETGLDAEGRIVARIEGSNCYGDA
                     KLAMIEQWLLREGLDRETVHIRFYSDHISDSVVHHWSDEPVATNAHQRLVKLARAEGW
                     EVLDWSR"
     gene            complement(1240..2544)
                     /locus_tag="VF589_10040"
     CDS             complement(1240..2544)
                     /locus_tag="VF589_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX8257964.1"
                     /translation="MEDESDLTGAAPRRRAKPDVLEIGGRKLNPSTLMMGHGYDPALS
                     EGSLKAPIFLTSTFVFENAAAGKRHFEGVTGKRPGGSEGLVYSRFNGPNQEILEDRLS
                     VWEDAEDALTFSSGMSAIATLLLTFVQPGDVIVHSGPLYAATETLIGRILGRFGVSWL
                     DFPAGAEQAEIEAVMERARSLGRVSLIYLESPANPTNVLVDVQAVAAARDGLFEADQR
                     PPIAIDNTFLGPLWSKPLEHGADLSVYSLTKYAGGHSDLVAGGVVGAKSLITPIRMMR
                     NTIGTITDPNSAWMLLRSLETLELRMTRAGENAAKVCAFFRDHPKVESVGYLGFLADD
                     RQADIYRRHCTGAGSTFSLYLRGGEREAFAFLDSLKIAKLAVSLGGTETLASAPAAMT
                     HLSVPDERKAALGISDNLVRISIGVEDAGDLIADFDQALAAI"
     gene            2676..4490
                     /locus_tag="VF589_10045"
     CDS             2676..4490
                     /locus_tag="VF589_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX8257965.1"
                     /translation="MQRRTFIAALPLTAAGLSLPARSAGQSGPGEPVSPALPATRLYP
                     DVEAGDRPVGASFATRSEVFGRNGAAASSHPLGTLAGIETLKKGGSAVDAAIAINASL
                     GFLEPTANGIGGDLYALLWDPKAAKVVGIAGSGRSPRGLSLETVRSRAKNGVLPPYGA
                     VTVSVPGTVDAWWRLHQRYGKLKWAELFEPTIAYAEGGVPLSPVVAYYMNRAMANYAK
                     QGAGIEEIVNAQATYASGERARAGEIFRNPDLARTYRAIAAGGRDAFYEGDIARTIEA
                     YFKRIGGWMTRADLAAHESEWTEPLATDYRGVRVHAIGANTQGLATLQMLNILERFDM
                     KQAGFQSPLSIHLQAEAKRLAYEDRARFYADPNFSRIPTDWLVSKDYAAERAKLIRPD
                     RINPNVRPGQAPSRGDTTYFTVADKDGMMVSLIQSNFRGMGSGLVADGLGFMFQDRGQ
                     LFTLQEGHPNFYAPGKRPFQTIIPGFATRGGKPWLSFGVMGGDMQPQGQAQVILNRTD
                     YGLDAQEAGDAPRWHHEGSSEAMGEDDKSIGSTGLLRLETGVPEATQRALAAMGWRLG
                     PSDGGFGRYSCVERRESGGERVYGAAADVRADGLGLAY"
     gene            complement(4487..5248)
                     /gene="pgeF"
                     /locus_tag="VF589_10050"
     CDS             complement(4487..5248)
                     /gene="pgeF"
                     /locus_tag="VF589_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX8257966.1"
                     /translation="MAVEAIRAALLGDLPHGFLGRRGGVSTGLCAGLNVGLGSGDDRD
                     AIRANRALAIAAVAPGAELVTLHQVHSADAIAVTSPFADDARPHADAMVTDRPGLALG
                     ILTADCTPILFADRDAGVIGAAHAGWKGAIGGVVAATVEAMERLGADRARIVAAVGPT
                     IARKSYEVDEAFFQRFTAEDARNERFFSAGKPGRHQFDLEAFVLARVGEAGIGRVEAL
                     GEDTYSQPDRFYSYRRATHRGEPDYGRQTSLIALP"
     gene            complement(5250..5768)
                     /locus_tag="VF589_10055"
     CDS             complement(5250..5768)
                     /locus_tag="VF589_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8257967.1"
                     /translation="MTGLTFRDAGPADAARLAELGRRTFTDTFGHLYKPEDLAAFLRS
                     HNEAGWRAELSDPAFAVRVGEADGELAAYAKLGPPSLPFEAVRPSIELRQFYLLKPWQ
                     GSGASHALMGWAIEQARARRAQDLYLSVFVDNQRARRFYERYGFTFVQTYAFIVGTHA
                     DEDHIMRLSLED"
     gene            complement(5765..6802)
                     /locus_tag="VF589_10060"
     CDS             complement(5765..6802)
                     /locus_tag="VF589_10060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8257968.1"
                     /translation="MSPADRLARLIAAAGAISVAQYMAEANAYYYAARDPFGTAGDFT
                     TAPEISQMFGELVGLWLADLWARAGKPPAHYVELGPGRGTLATDALRAMQGAGLDPDV
                     HLVETSPVLRAAQAERLPQARWHDDLATVPTDKPLLVVANEFFDALPIRQLIATESGW
                     RERLVGHDGERFFPAAGPPVPAERIPPDLRRTPPGTIVETSPASAAIARDLAGRLARQ
                     GGAALVIDYGHVATSAGETLQAVAGHAFADPWQAPGERDLTAHVDFQALAEAARGEGP
                     RISGPVPQGAWLDALGIGLRAAALAKAAPSRAGEIAAARARLTAPDQMGELFKVIAFT
                     AATWPEPAGFA"
     gene            complement(6951..7844)
                     /gene="lgt"
                     /locus_tag="VF589_10065"
     CDS             complement(6951..7844)
                     /gene="lgt"
                     /locus_tag="VF589_10065"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX8257969.1"
                     /translation="MILPQLASLAADATAALRWDSLGLHPVALDLGFFQLRWYSLAYL
                     AGIVLGWWYLLKLIERPGAPMARRHADDMVFYATLGILLGGRLGYVLFYRPGFYLENP
                     AEIVQLWDGGMSFHGGVIGVSLGILYMARKHSLDWLRIHDYVACCVPFGLFFGRLANF
                     VNGELWGRPTDVPWAMRFQERVNDLPVTGPPRHPSQLYEAGLEGLLLGLILWYMFWKT
                     DARYQPGRLVGTFILGYGVSRFLVELVRQPDVGLENLPWGLSMGQTLTVPMIAGGLYL
                     VATAKARRQRVEPIAGSESVA"
     gene            7912..9594
                     /locus_tag="VF589_10070"
     CDS             7912..9594
                     /locus_tag="VF589_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8257970.1"
                     /translation="MPSELDRKFDAVLAAVTGPGGRIAIGRDDAGRAIVTNLPPTLPG
                     LFDAFCALHAQTVAVVADGERLTFADLNAQATRLAKALAGGFGIGKGDRVAIAMRNAP
                     AWIVSYMAVLKAGGVAVLVNGWWQPEELRHGIELTEPKLVIADSQRANRIEGTGLALE
                     TVTLAVARPLDEALAPLLDRGSTEHPLPQVGPEDDATILFTSGSTGAAKGAVSTHRAV
                     TTGTYAYGLGLVTLLGILESEGRPPANPPRTLVNVPLFHVTGEVPVMLNSFVVGRGMV
                     LMAKWDAGEALRLIQEEKITYFVGVPTMSLELMQHPDREKYDLSTMTDIAAGGAARPV
                     SHVERLKQSFTAAQPALGYGLTETNAVGCSNFWSNYADKPASTGRPQAPIVEMAILGA
                     GDAHLPPGERGEIAIRTAANIRAYWRDPEASRAAFTADGYFRTGDIGYFDEDQYLFIV
                     DRKKDIIIRGGENISCQEVEAAIYAHPAVGEASVFGVPCDRLGEQPAAVVHSENGVSR
                     EELTAFLEVRLARFKLPQFIWFEEAPLPKLGTGKIDKVVLRERYTAELSRAG"
     gene            9653..11884
                     /locus_tag="VF589_10075"
     CDS             9653..11884
                     /locus_tag="VF589_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8257971.1"
                     /translation="MGAGDIGKGIDRRTLLIGGGVGAGLLIAWAVWPRSYSPNLRAAP
                     GETVFNAFLKIGNDGRVVVAVPQAELGQGVYTSLPQILADELGADWNTVAVEPAPLGP
                     LYANTFLAGEESAEGLPSSLQSVARWAAQEHATRTALMITGGSTSIRAFETPYREAGA
                     AARALLSKAAAERWGVDWEMLDTRGGFVVDGERKLSFGELAEAAARQELPADLPVRGG
                     LEGRLVGEELPRLDLPSKVDGSARFAGDVRLPDMVFASVRQGPFGATRLLRADRAAAD
                     RIPGVLATFENPGWVGAAATNWWAANRAVEAMRPVFETRGGLAGSADIRAALSSAMES
                     GESVRAFSRGEIGAAFGLVPGFRARYFAGLAPNAPLETLTATARLGGGRLEIWAPTQA
                     PSFARAAAARAAGLAEGQVTVYPMLVGGGYGRKLETEAIEQATAMAVRMKRPVQLVWS
                     RIEETMQDSFRPPAYAAMSASMIDGGRIMGWQARIAAPATTAQLIGRLQAGGKPAAPD
                     LSMVGGAVPPYAIPALAVDYAPAELPIRTGIWRSGSHSYTAFFTECFVDELARQAKVE
                     PLSFRMQMLGDNPRLARCLSTAAGLGGWDGGVPGSAMGIAVHSAFGSHAALLVEVAVD
                     QAQRIRVSRAVCAVDCGRVINPDIVKQLVEGGIIHGIAGATGNPINFERGLPNAQGFA
                     ELALPRLGDSPEVTVEIIESEEEPGGATELGVPAVAPAIANAIFASTGQRLRALPLVM
                     GGR"
     gene            11881..12888
                     /gene="hemH"
                     /locus_tag="VF589_10080"
     CDS             11881..12888
                     /gene="hemH"
                     /locus_tag="VF589_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX8257972.1"
                     /translation="MIPPPDHPAIPAPRIGVLLINLGTPNAPEPGAVRVYLKEFLSDP
                     RVVEIPQLVWQPILRGIILNTRPKKSAHAYKQVWSEEGSPLAAITARQAKALQREYGD
                     RLLVDHAMRYGRPAISERIEALKARGCERILLAPLYPQYCAATTATANDAAFAHLKTM
                     RWQPAMRTLPPYHDDASYIEALKETLTASLAALDFEPQAIVASFHGMPQRTLELGDPY
                     HCHCRKTARLLEAAMGRELTVAFQSRFGRAKWLEPATDTTLAALPGRGVTRVAIFAPG
                     FSADCLETLEELAIRGRETFLAAGGTHFAYLPCLNDSEAGIAMLRTIVDRELEGWLPA
                     Q"
     gene            12919..13641
                     /gene="phbB"
                     /locus_tag="VF589_10085"
     CDS             12919..13641
                     /gene="phbB"
                     /locus_tag="VF589_10085"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX8257973.1"
                     /translation="MARVAIVTGGTRGIGEAISLALRDIGMTVAANYAGNEEKARQFT
                     ERTGIKAYKWDVSDIDACMAGVQQVESELGPIDVLVNTAGITRDGTMKRMTRQAWDDV
                     IDVNLGGCFNMAKAVFEGMQARKFGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGARGGVTVNAIAPGYIDTEMVAAVPEDVLQKIVARIPVGRLGKAEEIARGVVFL
                     CGEDAGFVTGSTLSINGGQHMY"
     gene            13655..13882
                     /locus_tag="VF589_10090"
     CDS             13655..13882
                     /locus_tag="VF589_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX8257974.1"
                     /translation="MAGPVKRSMMIAGHATSISLEPIFWEALRAAAEAEGLPLNALVA
                     RIDAERVLGRDPPNLASAIRVWLFERLAARD"
     gene            complement(13879..18591)
                     /locus_tag="VF589_10095"
     CDS             complement(13879..18591)
                     /locus_tag="VF589_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX8257975.1"
                     /translation="MNADIVAGSGAPSAGSAVSDKPAGAAAAADLPYIPALRDALVRG
                     ALKDEIAGFSDEDRLEAARFVAACAQRRPPGIALVRLESMGVQLGKRRMRIGIVNDDM
                     PFLVDSIAGAIAARGITIHRLLHPVLCVERDAQGCLLSVGPLCDDLSKRESIMYIEAD
                     RTDARGRHELVGELHRVLADVRGSVTDWRALQAKMHADAETLRDEEGAALLHWFADGA
                     MTLLGYHVERPGEVPSEGLGLFRIPGDPTDEGGCDGAIAWFENGGAEPLVAKADRMST
                     VHRRVPFDLVVVPVRENGRITGVGVHAGLWTSQALTAPAEDVPVIRRRLGELDREFGF
                     DPNGHAGKALRHAVASLPRDLLVNLDPDAVRELVMTAMSLADRPRPTLVLLRSSLKGH
                     VFAFVWLPRDELTTRRRVAIGAMIEEAAQGRITSWSVELGDGDLALIRYTLSVSPATP
                     LPDVDVLDERLDAMVRGWEPSVEEALGALVGPARATRLAIGFVDDFPESYRVRTGPDE
                     AAQDVLRICELEGASARDARLYRRVGDGPSRLHLKTYRLAGIIPLSEAVPVLENFGFQ
                     VLEELPTPLDGGDLGYIHDFALEMPRGGDAGEVMSRAQVVERAIGAVLENRAENDGFN
                     QLLVGAGLGPRDVVLFRAWFRYLRQTGIAYSLATVVDALRHAPAVARGLIALFDAQHD
                     PSLESRRADAVRAAEETINRGLAQVAAIDEDRILRLIRGIVTATLRTNAFAESSREAL
                     AFKFDSCQVPNLPAPVPWREIWVYSPRVEGIHLRGGPVARGGLRWSDRRDDFRTEILG
                     LMKAQVVKNAVIVPTGAKGGFYPKQLPPTSDRDAWLAEGTESYRIFIRALLSITDNIV
                     EGAIVHPEGVTIRDRADPYFVVAADKGTATFSDVANGIAVSRGFWLGDAFASGGSQGY
                     DHKAMGITARGAWISVQRHFAEIGIDVQSQPIRVVGCGDMSGDVFGNGMLLSKAIKLV
                     AAFDHRHIFIDPDPDPARSWEERDRLFRLPRSSWDDYDKSLISAGGGVFPRTQKAIPL
                     TPEVRAALDLDVETIDPAGLISAILKAPADVIWFGGIGTYIKGKSESNAEVGDPGNDV
                     LRVNGGDLRAKAIGEGANLAITQAGRIEFAQAGGRINTDFIDNSAGVDCSDNEVNIKI
                     PLNREMAEGRLLEEDRNALLVAMTDDVSAIVLEDNRLQTLALSIAERGGAAALPPLVR
                     AIEILEESGRLNRVVEGLASNEDLLRRSQDDRGLTRPELAVLLSTSKMALQAAIEAGT
                     LTEDATLTHELCGAFPPLIQERHGEAILQHRLRREIIATKVANRFVNRLGITAVFSLT
                     EEEGASFGQAAAAFVAAERLFGMDDLWRRLDTIAVPEQLRLELFDQASKGLQLHIADM
                     LRCVPSAMKLQQIVDLLTPGIRKLSDKLDDLLREETRNEGSAQRAQLIAIGAPHDLVD
                     RIVRLFELNGAIGISMLGQKLSIDEVALTEAYTKLGEALGLDWAQGAANRFEAHDQWE
                     RLLTAGLARDFEQLRLEFLERRRGGDPREAVEQWVGVQGPRIDQFRRTVERARTASTT
                     TAPMLAQIATQARVLLAR"
     gene            complement(18735..19301)
                     /locus_tag="VF589_10100"
     CDS             complement(18735..19301)
                     /locus_tag="VF589_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257976.1"
                     /translation="MEDPLDGHTDGLIALLDLRARYVAQQVADRYKPKVARRYLPEFM
                     ALHRQFTGALRRGALVEAPAIEDRIFALSQAAGLSNDSWASRRLLKAAWVVAAELGIG
                     QRLVDFMLRRQRYDPVAESFGGIIGREYLQPEITDTIARALPDAESPRTEAGLKRRYL
                     AMLSEGQRVGYKSAQALLRKAVAGRPAA"
     gene            19504..19776
                     /locus_tag="VF589_10105"
     CDS             19504..19776
                     /locus_tag="VF589_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8257977.1"
                     /translation="MRQREKFAQVRALWGDDCWRCNKPMRFGGLPNCGKAATIEHLTP
                     LSKGGTWALDNLRLCHVGCNRHLGDHPREHKERMRINLRPAQGRPS"
     gene            19773..20261
                     /locus_tag="VF589_10110"
     CDS             19773..20261
                     /locus_tag="VF589_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257978.1"
                     /translation="MSKPAQAAGWLALLLLLATAPADEPRPGTVFERRTNASQPVLVS
                     GWRHLGGGYWGDANRDECCFDLFRSGSASILAIVEPVARDARGGMLSSRILETHRMKL
                     EPNEDWATCSLAGRAALLAALDAKTGRVRGVFMGRDGFEAATWAARAPAPEETTYCEE
                     GG"
     gene            complement(20314..21192)
                     /locus_tag="VF589_10115"
     CDS             complement(20314..21192)
                     /locus_tag="VF589_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX8257979.1"
                     /translation="MNRDQSGNSSRDRLIALLIDADNVSHGKIAAMLAELSKYGVANI
                     RRAYGDWASPSMKGWTARLHEHAIRPIQQFSYSKGKNATDMALVIDAMELLYTQKPDA
                     FCIASSDADFTPLVMQLRANGHEVYGFGARKTPDPFVNACSTFLYLENLVEEAPAAAA
                     PAREPRPAPAARPAPASSSKSAPAPAAKPAPAPAAKPADAASKPPLAQDARLVAVLRG
                     AVEATAGDDGWAPLDAAGSAARRQAPIDPRNYGARNFPKLFEAVGLFDIVKAEETGHS
                     YVSDKRNKDRTPRPRF"
     gene            complement(21657..22184)
                     /locus_tag="VF589_10120"
     CDS             complement(21657..22184)
                     /locus_tag="VF589_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX8257980.1"
                     /translation="MSISKPIAATLIALAAALAAAPAQAEILTFQAKLDGKYGDAPTG
                     SAATGSARIKVDTESRRVSVDMIVDGITTEALWDKLVAGPIGPIHFHKYATPAGGDSV
                     LVLPVAFGPGYTSTKSGMRVAMKDYDYSAGAALLKSTLTFDQFVAAMRGGLVILNVHT
                     DAFNPGEISGRVTEG"
     gene            complement(22313..23083)
                     /locus_tag="VF589_10125"
     CDS             complement(22313..23083)
                     /locus_tag="VF589_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257981.1"
                     /translation="MRRTPPFPRLCLLAAAAALFQLLAAPAQACSVADSYRVPTNVEL
                     AADAEIILLARVESGTTGTAHPNITMTVTPIELLKGRLPMDKPLTLPGAIAEPRFALL
                     SSPLELEQAHPLAYIGSCTRFMFVRGATVLFFLNPIEKAMKGRQVPAPIRGMLVPAGS
                     AFSRWAEDVLSAGSPWVRATRTYIAAAALPADRQKAMLVAERDRFRAAADQESRVVAD
                     DIDRQLAGPNKPWNQLMQEAISKRKSRGQGSPDPLRGN"
     gene            complement(23188..23838)
                     /locus_tag="VF589_10130"
     CDS             complement(23188..23838)
                     /locus_tag="VF589_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257982.1"
                     /translation="MDIPLPPPSGDPPPNDPSAGTPRAAIPFDPVPVQPRRDGWTAER
                     QRRFIGVLAETGVVRFAAAAAGMSESSAHRLAQRPDAQQFCNAWDAALRMAARPAAAK
                     LYEYALDGMTETVWRDGEIVYQRRRPSEKALIFLLSRLDPVRFGRPPAAAPAATDRDG
                     LPLDPLAETAGLFDLFLDNLRDLPPEEGYEAEAKGLRPDPPTNPPNPPQPPEPGHG"
     gene            24064..24429
                     /locus_tag="VF589_10135"
     CDS             24064..24429
                     /locus_tag="VF589_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257983.1"
                     /translation="MTDQRQSDDGKEGIPCPFGDGGARNWKAWVNAMPGADAQLIVIG
                     EVNAPQGYTGRLRFGIADKRKPPTQYLELNLVEEAGAPDGWREIRAQGKALGREYAAV
                     VILCHGDELARITEVPVVE"
BASE COUNT         3792 a         8058 c         9003 g         3596 t
ORIGIN      
        1 ctggacgacc gcgttgaacc ggaagcggcg cggatgggtg gccacgttcc aggccgagac
       61 cagcaactcg ccgaaaaagc cgagcagccc gagcatggtg ccggcggcga gggcggtggc
      121 ttcgccgacc tcgtccatga cccggtagag cggtggtctc cgatccgggc gcatcttgac
      181 cggctggtcg gcctggccga cctgctccag caacaccgcc tcgtcatggc cgacgccgac
      241 caggcgggcg tcgcggtcgc gcgccagccg gtggaccagc cacgcgccga ccgtgtccat
      301 ccgctccacg ttcgacagat cgaggatcag ccgctcgccg tgaatctggt cgagccgctc
      361 gggcaagccg tcaatgccgg caatggtcat cccgccggtg aagcggacaa ccgaagtatc
      421 gccttcaagc gtctgggtga aatcggccgg ccggtacatc gcggcgcgac actgcttcaa
      481 ttcacgggcg gcggcaagac gtgagggcgg tggacaagcc cggccggctg tgcttgccgg
      541 gcagcgatga aggccgcgct cgccatctac gacatggacc gcacgatcac ccggcgcgcc
      601 acctacacgc cgttcctgat ccatgcggcg ctgcggctgg caccgtggcg gctagcgctg
      661 gtgccggtgg cggtgctcgc ggccttggcc tatctcgcgc ggctgatcga ccgggcgcgg
      721 ctcaaggaag tcaattaccg gctgctgatc ggcgggagcg tggaaccctc ccgcctcgat
      781 ccggtcatcc agagcttcgc cgagcggcag gtggcggcga acatcatgcc cggcgcccgg
      841 gcgagcatcg ccgccgaccg cgccgctggc cggcggctgg tgatggcgac ggccagctac
      901 cgcatctacg cggccgccat cgccgagcgg ctcggcttca aggacgtgat cgcgaccgag
      961 accgggctcg acgccgaggg acgtatcgtc gcccggatcg agggcagcaa ttgttatggc
     1021 gacgccaagc tggcgatgat cgagcaatgg ctgctgcgcg aagggctgga ccgggaaacg
     1081 gtccacatcc gcttctattc ggaccatatc tccgattccg tcgtccacca ttggtcggac
     1141 gagccggtgg cgaccaacgc ccaccagcgg ctggtcaagc tcgcccgggc cgaaggctgg
     1201 gaagtgctcg actggagccg gtgatccggc cccgtcaggt cagatcgccg ccagcgcctg
     1261 gtcgaagtcg gcgatgaggt cgcccgcatc ctcgaccccg attgatatgc ggaccagatt
     1321 gtcggaaatg cccagcgccg ccttgcgctc gtcgggcacc gacaaatggg tcatcgccgc
     1381 gggcgcgctg gcaagcgtct cggtgccgcc gagcgagaca gcgagcttgg cgatcttgag
     1441 cgaatcgaga aaggcgaagg cttcgcgttc gccgccgcga agatagagcg agaaggtgga
     1501 gccggcgccg gtgcagtggc ggcggtagat gtcggcctgg cggtcgtcgg cgaggaagcc
     1561 gagatagccg acgctttcga ccttgggatg atcgcggaag aaggcgcaga ccttggcggc
     1621 attctcgccg gcccgggtca tgcgaagctc gagcgtctcg aggctcctga gcagcatcca
     1681 ggcgctgttg gggtcggtga tggtgccgat cgtgttgcgc atcatgcgga tcggggtgat
     1741 cagcgacttc gccccgacca cgccgccggc gacgaggtcg ctgtggccgc cggcatattt
     1801 ggtgagcgaa tagaccgaga ggtcggcgcc atgttcgagc ggcttcgacc agagcggccc
     1861 gaggaaggtg ttgtcgatcg cgatcggcgg gcgctgatcg gcttcgaaca aaccgtcgcg
     1921 cgcggccgcc accgcctgca cgtcgacgag gacgttggtc gggttggcgg ggctttcgag
     1981 atagatgagc gagacccggc ccaggctgcg ggcgcgctcc atcaccgcct cgatctcggc
     2041 ctgctccgcg ccggccggga aatcgagcca gctcaccccg aagcggccga ggatgcggcc
     2101 gatcagcgtc tcggtcgccg cataaagcgg gcccgaatgg acgatgacgt cgccgggctg
     2161 gacgaaggtc agcagcaagg tggcaatggc ggacatgcca ctcgagaaag tgagcgcgtc
     2221 ctcggcatcc tcccagacgc tgaggcgatc ctcgagaatc tcctggttgg ggccgttgaa
     2281 gcgcgaatag accaggccct cggacccgcc ggggcgcttg ccggtgacgc cttcgaagtg
     2341 gcgcttgccg gcggcggcat tctcgaacac gaaggtggag gtgaggaaga tcggcgcctt
     2401 cagcgaccct tccgacaaag ccgggtcata gccatggccc atcatcagcg tcgagggatt
     2461 gagcttgcgc ccgccgatct cgagcacgtc cggcttggcg cggcggcgcg gggcggcgcc
     2521 ggtgaggtcg gattcgtctt ccatcatgag gctcccaggg caggttgccc gctgcctaga
     2581 ccggagcggc ggcggcggca actggcacag accgcggagc acgcttgcac ctttccaccg
     2641 gcttcgccac tggaggcgaa gccacggaga tcgttatgca aagacgcacc ttcattgccg
     2701 cgctgccgct gacggcggcg gggctttcgc tgcccgcccg ctcggccggg caaagcggac
     2761 cgggcgaacc ggtcagcccc gctttgcccg ccacccggct ctatccggat gtcgaggccg
     2821 gcgaccggcc ggtgggggcg agcttcgcca cccggtccga ggtgttcggg cgcaacggcg
     2881 ccgccgcttc ctcgcacccg ctcggcacgc tcgccgggat cgagacgctg aaaaagggcg
     2941 ggtccgcggt cgatgccgcg atcgcgatca acgccagcct cggcttcctc gagccgaccg
     3001 ccaacggcat cggcggcgac ctatacgccc tgctgtggga cccgaaggcg gcgaaggtgg
     3061 tggggatcgc cggctcgggc agatcgccgc gcgggctcag cctggagacg gtgcgctcgc
     3121 gggcgaagaa tggcgtgctg ccgccttatg gcgcggtgac ggtgtcggtg ccgggaacgg
     3181 tcgacgcctg gtggcggctg catcagcgct acggcaagct caagtgggcc gaattgttcg
     3241 agcccaccat cgcctatgcc gagggcggtg tgccgctgtc gccggtggtc gcctattaca
     3301 tgaaccgggc gatggccaat tacgccaagc agggcgcagg gatcgaggag atcgtcaacg
     3361 cgcaggcgac ctatgcgtcg ggcgagcgcg ccagagccgg cgagatcttc cgcaacccgg
     3421 atctcgcccg cacctaccgc gcgatcgcgg cgggcggccg cgacgctttt tacgagggcg
     3481 acatcgcccg cacgatcgag gcctatttca agcggatcgg cggctggatg acgcgcgcgg
     3541 acctggccgc gcacgagagc gaatggaccg agccgctggc gaccgactat cgcggcgtcc
     3601 gcgtccacgc gatcggcgcg aacacgcaag gcctggcgac gctgcagatg ctgaacatcc
     3661 tcgagcggtt cgacatgaag caggcgggtt tccagtcgcc gctgtcgatc cacctccagg
     3721 ccgaggccaa gcggctcgcc tacgaagacc gcgcgcgttt ttatgccgac ccgaatttct
     3781 cgcggattcc gacggactgg ctggtctcca aggactatgc cgccgagcgg gcgaagctga
     3841 tccggcccga ccggatcaac ccgaatgtcc ggccgggcca ggcgccgtcg cgcggcgaca
     3901 cgacctattt caccgtcgcc gacaaggacg ggatgatggt gtccctcatt cagtccaact
     3961 tccgcggcat gggatcggga ctggtggcgg acgggctcgg cttcatgttc caggaccgcg
     4021 gccagctttt cacgctgcag gaggggcacc cgaatttcta tgcgcccggc aagcggccgt
     4081 tccagacgat cattcccggc ttcgccaccc gcggcggcaa gccgtggctt tcgttcggag
     4141 tgatgggcgg cgacatgcag ccgcagggcc aggcccaggt gatcctcaac cgcaccgact
     4201 acggcctcga cgcgcaggag gcgggcgatg cgccgcgctg gcaccatgag ggctcttccg
     4261 aagcgatggg cgaggacgac aagagcatcg gcagcaccgg attgctgcgg ctggagaccg
     4321 gcgtccccga ggcgacccag cgggcgctgg cggcaatggg ctggcggctt ggcccgtcgg
     4381 acggcggctt cggccgctat tcctgcgtgg agcggcggga aagcggcggc gaacgcgtct
     4441 acggcgctgc ggccgacgtg cgcgcggacg ggctggggct ggcttactag ggcagcgcga
     4501 tcagcgaggt ttggcggcca tagtccggct cgccgcgatg ggtggcgcgg cggtagctgt
     4561 agaagcggtc cggctgggaa tacgtgtcct cgccgagcgc ctcgacacgg ccgatcccgg
     4621 cctcgccgac acgagcgagg acgaaggctt cgaggtcgaa ctggtggcgg cccggcttgc
     4681 ccgccgagaa aaagcgttcg ttccgcgcat cctcggccgt gaagcgttgg aagaaggcct
     4741 cgtccacctc gtaggatttg cgcgcgatgg tcgggccgac ggcggcgacg atccgcgccc
     4801 ggtcggctcc gagccgctcc atcgcctcga cggtcgcggc gacgacgccg ccgatcgcgc
     4861 ccttccaacc ggcatgggcg gcgccgatca cgcccgcatc gcggtcggcg aacaggattg
     4921 gcgtgcagtc ggcggtgagg atgccgagcg ccagccccgg ccggtccgtc accatggcgt
     4981 cggcatgcgg gcgggcatcg tcggcgaaag ggctagtgac ggcgatggca tctgccgaat
     5041 gcacctggtg cagcgtcacc agctcggcgc ccggcgcgac ggccgcgatg gccagcgcgc
     5101 ggttggcgcg gatcgcatcg cggtcgtcgc ccgagccgag gccgacgttg agaccggcgc
     5161 aaaggccggt cgacacgccg ccgcgccggc cgaggaagcc gtgcgggaga tcgccgagca
     5221 gggccgcgcg gatcgcttcg acggccatct caatcctcca gcgacaggcg catgatatgg
     5281 tcctcgtcag cgtgggtgcc gacgatgaac gcataggtct gcacgaaggt gaagccgtag
     5341 cgctcgtaaa agcggcgcgc gcgctgattg tcgacgaaca ccgacagata aaggtcctgg
     5401 gcgcggcgcg cccgcgcctg ttcgatcgcc cagcccatca aggcgtgcga ggcgccgctg
     5461 ccctgccagg gtttcagcag gtagaattgg cgcagctcga tcgacggccg gaccgcctcg
     5521 aacggaagcg aaggcgggcc gagcttggcg taagccgcca actcgccatc ggcctcgccc
     5581 acgcggacgg cgaaggcggg gtcggacagc tccgcgcgcc agccggcttc attgtgggag
     5641 cgcaggaagg cggcgagatc ctccggcttg tagaggtggc cgaaagtgtc ggtgaaggtg
     5701 cggcgtccga gctcggcaag ccgggccgca tcggccggcc cggcgtcgcg gaaggtcagc
     5761 ccggtcatgc gaagccggcg ggttccggcc aggtcgcggc ggtgaaggcg atcaccttga
     5821 agagctctcc catctgatcg ggtgcggtca gccgcgcgcg ggcggcggcg atttcccctg
     5881 cgcgggacgg ggcggccttg gcaagggccg cggcgcgaag gccgatgccc aaggcatcga
     5941 gccaggcgcc ttgcgggacc gggcccgaaa tccggggccc ctccccgcgc gctgcctcag
     6001 ccagcgcctg gaagtcgaca tgggcggtga ggtcgcgctc gccgggcgcc tgccagggat
     6061 cggcaaaggc gtggccggcg accgcctgca gcgtctcccc tgcgctggtg gcgacgtggc
     6121 catagtcgat gacgagcgcg gcgccgccct gccgggcgag ccggccggcg aggtcgcggg
     6181 cgatggcggc cgaggcggga gaggtctcga cgatggtgcc gggaggggtg cgccgcaggt
     6241 cgggcggaat gcgctccgca gggacgggcg gaccggcggc agggaagaag cgctcaccgt
     6301 catggccgac cagccgctcg cgccagccgc tttcggtggc gatcagctgg cggatcggga
     6361 gcgcgtcgaa gaactcgttg gcgacgacca gcagcggctt gtccgtggga acggtggcga
     6421 gatcgtcatg ccagcgcgcc tgcggcaggc gctcggcctg ggcggcgcgg aggaccgggc
     6481 tggtttcgac gagatggaca tccggatcga gcccggcgcc ttgcatggcg cggagcgcgt
     6541 cggtggcgag cgtgccgcgg ccggggccga gctcgacata atgcgccggc ggcttgccgg
     6601 cgcgcgccca caggtcggcg agccacagcc cgaccagctc cccgaacatc tggctgattt
     6661 ccggcgcggt ggtgaagtcg cccgccgtgc cgaacgggtc gcgggcggca taataataag
     6721 cgttggcctc ggccatatac tgggcgaccg aaatcgcgcc ggcggcggcg atcaggcggg
     6781 cgaggcgatc ggcgggggac atcgtgccct caccctccca cctgcctgcg gcaggcgggc
     6841 ccctccctct cccctaaagg gagaggggaa gtcggggcgc ctgctagaac ccctctccca
     6901 tttagggaga gggaggggcc cgccgcgaag cggtgggagg gtgagggcac ttaggccacg
     6961 ctctccgagc cggcgatcgg ctcgacgcgc tggcggcggg ccttggcggt ggcgaccaga
     7021 tagaggccgc cggcgatcat ggggacggtg agggtctggc ccatggagag gccccaggga
     7081 aggttctcga gacccacatc gggctggcgc accagctcga ccaggaagcg gctgacgccg
     7141 tagccgagga tgaaggtgcc gacgaggcgg ccgggctggt agcgggcgtc ggtcttccag
     7201 aacatgtacc acaggatgag gccgagcagc aggccttcga ggcccgcctc gtagagctgg
     7261 ctcgggtggc ggggcggccc cgtcaccggc aggtcgttca cgcgctcctg aaaccgcatc
     7321 gcccagggca catcggtcgg gcgcccccac agctccccgt tcacgaaatt ggcgaggcgg
     7381 ccaaagaaca ggccgaaggg gacgcagcag gcgacgtaat cgtggatcct cagccaatcg
     7441 aggctgtgct tgcgggccat gtagaggatg ccgaggctga cgccgatgac gccgccgtgg
     7501 aacgacatgc cgccgtccca gagctgcacg atctcggcgg ggttctcgag gtagaagccg
     7561 ggccggtaga acaggacgta gccgagccgc ccgccgagca ggatgccgag cgtcgcgtag
     7621 aagaccatgt cgtcggcatg gcggcgcgcc atcggggcgc cgggccgctc gatcagcttc
     7681 aggaggtacc accagccgag gacaatgccg gcgagatagg cgagcgaata ccagcggagc
     7741 tgaaagaagc cgaggtcgag ggcgaccggg tggaggccga gactgtccca gcgcaaggct
     7801 gccgtagcgt cagccgcgag gctcgcgagt tgtggcagga tcaaggcttt tcccccaggc
     7861 tttccgccct catagtggtg gcgaaaagaa acggaaagag cgggagattc gatgccgagc
     7921 gagcttgacc gcaagttcga cgccgtgctg gcggcggtga ccgggccggg cgggcggatc
     7981 gcgatcggcc gcgatgacgc cggccgggcg attgtcacca atttgccgcc gaccctgccg
     8041 ggcctgttcg acgccttttg cgcgctccac gcccagacgg tggcggtggt cgcggacggg
     8101 gagcggctga ccttcgccga cctcaatgcg caggcgaccc ggctggccaa ggcgctggcg
     8161 gggggcttcg gcatcggcaa gggcgaccgg gtcgcgatcg cgatgcgcaa cgcgccggcc
     8221 tggatcgtca gctatatggc ggtgctgaag gccggcgggg tggcggtgct ggtcaacggc
     8281 tggtggcagc cggaggagct gaggcacggg atcgagctga ccgagccgaa gctggtgatc
     8341 gccgacagcc agcgcgcgaa tcggatcgag ggcaccgggc tggcgctgga gaccgtgacg
     8401 ctggcggtgg cgcggccgct cgatgaggcg ctggcgccgc tgctcgatcg cggctcgacc
     8461 gagcacccgt tgccgcaagt ggggccggag gacgacgcca cgatcctgtt cacctcgggc
     8521 tccaccgggg cggccaaggg cgccgtctct acccaccgcg ccgtcaccac cggcacctat
     8581 gcctatgggc tcggcctcgt caccctgctc ggcatcctgg agagcgaagg ccgccctccc
     8641 gccaacccgc cccggacctt ggtcaacgtg ccgctgttcc acgtcaccgg cgaagtcccg
     8701 gtgatgctga acagcttcgt cgtcgggcgc ggcatggtgc tgatggcgaa atgggacgcc
     8761 ggcgaggcgc tgcgcctcat ccaggaggag aagatcacct atttcgtcgg tgtgccgacc
     8821 atgagccttg agctgatgca gcacccggac cgggagaagt acgatctctc gaccatgacc
     8881 gacatcgcgg cgggcggcgc cgccaggccg gtcagccatg tcgagcggct gaagcaaagc
     8941 ttcaccgccg cccagccggc gctcggctac gggctcaccg agaccaatgc ggtcggctgc
     9001 agcaacttct ggtccaatta tgccgacaag cccgcctcga ccgggcggcc gcaggcgccg
     9061 atcgtcgaga tggcgatcct cggcgccggc gacgcgcatc ttccgccggg cgagcgcggc
     9121 gagatcgcga tccgcaccgc cgccaacatc cgcgcttatt ggcgcgatcc cgaggcgagc
     9181 cgcgccgcct tcaccgccga cggctacttc cgcaccggcg acatcggcta tttcgacgag
     9241 gaccaatatc tgttcatcgt cgaccggaag aaggacataa tcatccgcgg cggcgagaat
     9301 atcagctgcc aggaagtgga ggcggcgatc tacgcccatc ccgctgtcgg agaagccagc
     9361 gtgttcggag tgccgtgcga ccggctgggc gagcagccgg cggcggtggt ccacagcgag
     9421 aacggcgtgt cgcgggagga gctgaccgcc tttctcgaag ttcgcctcgc ccgcttcaag
     9481 cttccgcaat tcatctggtt cgaagaggcg ccgctgccca agctcggcac cggcaagatc
     9541 gacaaggtgg tgctgcgcga gcgctacacg gccgagctga gccgcgccgg gtgagccgcc
     9601 aatctttcca gcggtctagc ggtgcggcag cgaggtcttt atcaacccgc cgatgggcgc
     9661 cggcgacata ggaaaaggta tcgaccggcg gaccctgctg atcggcggcg gcgtcggggc
     9721 cggcctgctg atcgcctggg cggtctggcc gcgcagctat tcgcccaatc ttcgcgccgc
     9781 ccccggcgag acggtgttca acgccttcct caagatcggc aatgacggcc gcgtggtggt
     9841 ggcggtgccg caggccgagc tcggccaggg cgtctacacc agcctgccgc aaatcctcgc
     9901 cgacgagctc ggcgccgact ggaacacggt cgcggtggag ccggcgccgc tcggcccgct
     9961 ttacgctaac accttcctcg ccggcgagga atcggccgaa ggtctgccgt cgagcctgca
    10021 gagcgtggcg cgctgggccg cgcaggaaca tgccacgcga accgcgctga tgatcaccgg
    10081 cggatcgact tcgatccgcg ccttcgagac gccctatcgc gaggccgggg cggcggcccg
    10141 cgccctgctc agcaaggcgg ccgccgaacg ctggggcgtg gattgggaaa tgctcgacac
    10201 gcgcggcggc ttcgtggtcg acggcgagcg caagctgagc ttcggcgagc tggccgaggc
    10261 ggcggcgcgg caggagctgc ccgccgacct gccggtccgg ggcgggctcg aaggccggct
    10321 ggtcggcgaa gagctcccgc ggctggacct gccgagcaag gtcgacggct cggcgcgctt
    10381 cgccggcgac gtgcgcctcc ccgacatggt gttcgcctcg gtccggcagg ggccgttcgg
    10441 ggcgacccgg ctgctgcgcg ccgaccgcgc cgccgccgac cgcattcccg gcgtgctcgc
    10501 gacgttcgag aatcccggct gggtgggcgc ggcggcgacc aattggtggg cggcgaaccg
    10561 cgcggtggag gcgatgcggc cggtgttcga gacgcgtggc ggcctcgccg gcagcgccga
    10621 tatccgggcg gcgctttcct ccgccatgga gagcggcgag agcgtgcgcg cgttcagccg
    10681 cggcgagatc ggcgcggcgt tcgggctcgt gccgggcttt cgcgcgcgct actttgccgg
    10741 gctcgcgccg aacgcgccgc tggagacgct gaccgcgacc gcgcggctcg gcggggggcg
    10801 gctcgaaata tgggctccga cccaggcgcc gtcgttcgcg cgggcggcgg cggcgcgcgc
    10861 ggccgggctc gccgaagggc aggtcaccgt ctatccgatg ctggtcggcg gcggctacgg
    10921 gcgcaagctg gagaccgaag cgatcgagca ggcgacggcg atggcggtga ggatgaagcg
    10981 gccggtgcaa ttggtctggt cgcggatcga ggagacgatg caggacagct tccgcccgcc
    11041 cgcttatgcg gcgatgagcg cgagcatgat cgacggcggc cgcatcatgg gctggcaggc
    11101 gcggatcgcg gcgccggcga cgaccgcgca attgatcggg cggctgcagg ccggcgggaa
    11161 gccggcggcg ccggaccttt cgatggtcgg cggcgcggtg ccgccatatg cgatccccgc
    11221 attggcggtc gactatgcgc cggccgagct gccgatccgg accggcatct ggcggtcggg
    11281 ctcgcacagc tacacggcct ttttcaccga atgcttcgtc gacgagctgg cgcggcaggc
    11341 caaggtcgag ccgctttcct tccggatgca gatgctcggc gacaatccgc gcctcgcccg
    11401 ctgcctgtcg acggcggccg gattgggcgg ttgggacggc ggcgtgccgg gcagcgcgat
    11461 gggaatcgcc gtgcacagcg cgttcggctc gcacgccgcc ttgctggtcg aggtcgcggt
    11521 cgaccaggcc cagcggatcc gcgtcagccg ggcggtctgc gcggtcgatt gcgggcgggt
    11581 gatcaatccc gacatcgtca agcagctcgt cgaaggcggg atcattcacg gcattgccgg
    11641 cgccaccggc aacccgatca atttcgagcg cgggctcccg aatgcgcagg ggttcgccga
    11701 gctcgcgctg ccgcggctcg gcgacagccc cgaagtcacg gtcgagatca tcgagagcga
    11761 ggaagagccg ggcggcgcca ccgagctcgg cgtgccggcg gtggcgccgg ccatcgccaa
    11821 cgcaatcttc gcatcgaccg ggcagcggtt gcgcgcgctg ccgctggtga tgggcggacg
    11881 atgatccctc cccccgatca tcccgcaatc ccggcgcccc gcatcggcgt gctgctgatc
    11941 aacctcggca cgcccaatgc gcccgagccc ggggcggtgc gggtctatct gaaggaattc
    12001 ctgtcggacc cgcgggtggt cgagatcccg caactggtgt ggcagccgat cctccggggc
    12061 atcatcctca acacccggcc gaagaagtcg gcccatgcct ataagcaggt gtggagcgag
    12121 gaaggctcgc cgctggccgc gatcacggcg cgccaggcca aagcgctgca gcgggaatat
    12181 ggcgatcggc tgctggtcga ccatgccatg cgctacggcc gcccggccat tagcgagcgg
    12241 atcgaggcgc tgaaggcgcg cggctgcgag cgcatcctgc tggcgccgct ctatccgcaa
    12301 tattgcgccg cgacgacggc gaccgccaac gatgccgcct tcgcgcacct caagacgatg
    12361 cgctggcagc cggcgatgcg gacgctgccg ccttatcatg acgacgcttc ctacatcgag
    12421 gcgttgaagg agactctgac cgccagcctc gcggcgctgg atttcgagcc gcaggcgatc
    12481 gtcgccagct tccacggcat gccgcagcgc acgctcgagc tcggcgaccc ttatcattgc
    12541 cactgccgca agaccgcgcg gctgctcgaa gcggcgatgg ggcgggaact gaccgtcgcc
    12601 ttccagtcgc gcttcggccg ggccaagtgg ctggagcccg ccaccgacac gacgctggcg
    12661 gcgctgcccg gacgcggcgt cacgcgggtc gccatcttcg cacccggctt ttccgccgat
    12721 tgcctggaga cgctggaaga gctcgccatt cgcggccgcg agaccttcct cgccgccggc
    12781 ggcacccact tcgcctacct cccctgcctc aacgacagcg aagccggcat cgcgatgctg
    12841 cgcacgatcg tggaccgcga acttgaaggt tggctgcccg cgcaataagg gcgggcgaaa
    12901 ccataaagga gagcatctat ggcacgcgtc gcaatcgtca ccggcggaac ccgcggcatc
    12961 ggcgaggcga tcagcctcgc gctccgcgac atcggcatga ccgtcgccgc caattatgcc
    13021 ggcaatgagg agaaggcgcg gcagttcacc gagcggaccg gcatcaaggc ctataaatgg
    13081 gacgtgtccg atatcgacgc ctgcatggcc ggcgtccagc aggtggagag cgagctgggg
    13141 ccgatcgacg tgctggtcaa cactgccggc atcacccgcg acgggacgat gaagcggatg
    13201 acgcggcagg cgtgggacga cgtcatcgac gtcaatctcg gcggctgctt caacatggcc
    13261 aaggccgtgt tcgaaggcat gcaggcgagg aagttcggcc ggatcgtcaa tatcggctcg
    13321 atcaacgggc aggccggcca gtacggccaa gtcaattacg ccgccgccaa atcgggcatc
    13381 cacggcttca ccaaggcgct tgcgcaggag ggcgcgcgcg gaggggttac ggtgaacgcg
    13441 atcgcgcctg gctatattga caccgagatg gtggcggccg tgcccgaaga cgtgctccag
    13501 aagatcgtcg cgcggattcc ggtcggccgg ctcggcaagg ccgaggagat cgcccgcggg
    13561 gtggtcttcc tgtgcggcga agatgccggg ttcgtcaccg gcagcacgct ttcgatcaac
    13621 ggcggccagc acatgtattg aggccaatgc gggcgtggcg ggaccggtga aacgatcgat
    13681 gatgatcgcc ggtcacgcga cctcgatcag cctcgagccg atcttctggg aggcgcttcg
    13741 cgcggccgcg gaggccgaag ggctgccgct caacgcgctg gtcgcccgca tcgatgccga
    13801 gcgtgtcctc ggccgcgacc ctccgaacct ggccagcgcg atccgggtct ggctgttcga
    13861 acggcttgcc gcgcgcgact agcgcgccag cagcaccctc gcctgggtgg cgatctgggc
    13921 cagcatcggc gcggtggtcg tcgacgccgt ccgcgcccgt tcgacggtgc ggcggaactg
    13981 atcgatgcgc ggcccctgca cgccgaccca ttgctcgacc gcctcgcgcg ggtcgccgcc
    14041 gcgccggcgc tcgagaaatt cgagccgcag ctgctcgaag tcgcgcgcca ggccggcggt
    14101 cagcagccgc tcccactggt cgtgcgcttc gaagcggttg gcggcacctt gcgcccagtc
    14161 gagcccgagc gcctcaccga gcttggtata ggcttcggtg agcgcgacct cgtcgatgct
    14221 gagcttctgg ccgagcatcg aaatgccgat cgcgccgttg agctcgaaca gccggacgat
    14281 gcggtcgacc aggtcgtggg gcgcgccgat ggcgatgagc tgggcgcgct gggcgctgcc
    14341 ctcgttgcgg gtctcctcgc gcagcaaatc gtcgagcttg tcgctgagct tgcggatgcc
    14401 gggggtcagc aggtcgacga tctgctgcag cttcatcgcc gaggggacgc agcgcagcat
    14461 gtcggcaatg tgcagctgaa ggcccttgga cgcctggtcg aacagctcga gccggagctg
    14521 ctcggggacc gcgatggtgt cgaggcggcg ccacagatcg tccatgccga acagccgctc
    14581 ggccgcgacg aaggctgccg ccgcctggcc gaacgaggcg ccctcctcct cggtcagcga
    14641 gaagacggcg gtgatgccga gccggttgac gaagcggttg gcgaccttgg tggcgatgat
    14701 ctcgcggcgc aggcggtgct ggaggatcgc ctcgccatgc cgctcctgga tcagcggcgg
    14761 gaaggcgccg cacaattcgt gggtcagcgt cgcgtcctcg gtgagcgtgc cagcctcgat
    14821 cgccgcctgc agcgccattt tggacgtcga cagcaacacg gccagctcgg gccgggtgag
    14881 gccgcgatcg tcctgcgagc gccgcagcaa atcctcgttg ctggcaagcc cttcgacgac
    14941 acggttcagc cgcccgcttt cctcgaggat ctcgatcgcg cggaccaaag gcggcagcgc
    15001 cgcggcgccg ccgcgctcgg cgatcgacag cgccagcgtc tgcagccggt tgtcctcgag
    15061 cacgatcgcc gagacgtcgt cggtcattgc gacgagcagg gcgttgcggt cttcctccaa
    15121 caatcgacct tcggccatct cccggttgag cgggatcttg atgttgacct cgttatccga
    15181 gcagtcgacg ccggccgaat tgtcgatgaa gtcggtgttg atccgcccgc ccgcctgggc
    15241 gaactcgatc cggccggcct gggtgatggc gaggttggcc ccctccccga tcgccttggc
    15301 gcgaagatcg ccgccgttga cccggaggac gtcgttgccg ggatcgccga cctcggcgtt
    15361 gctctcggat ttgcccttga tgtaggtgcc gatgccgccg aaccagataa catcggccgg
    15421 ggccttgagg atcgcgctga tcagtccggc cgggtcgatc gtctcgacgt cgaggtcgag
    15481 cgcggcgcgg acctcggggg tcaggggaat cgccttctgc gtccgtggga acacgccgcc
    15541 gccggcggag atgagcgact tgtcgtaatc gtcccagctc gatcgcggca gcctgaacag
    15601 ccggtcgcgc tcttcccagc tgcgggcggg gtccggatcg ggatcgatga agatgtggcg
    15661 gtggtcaaat gccgcgacca gcttgatcgc cttggagagc agcatgccgt tgccgaagac
    15721 gtcgcccgac atgtcgccgc agcccacgac ccggatcggc tgggactgaa cgtcgatgcc
    15781 gatttcggcg aagtggcgct ggaccgaaat ccaggcgccg cgggcggtga tgcccatcgc
    15841 cttgtggtcg tagccctggc tgccgcccga ggcgaacgcg tcgccgagcc agaagccgcg
    15901 cgacacggcg atgccgttgg cgacgtcgga gaaggtcgcg gtgcccttgt cggcggcgac
    15961 cacgaaatag ggatcggcgc ggtcgcggat ggtgacccct tccggatgga cgatggcacc
    16021 ttcgacgata ttgtcggtga tcgagagcag ggcgcggatg aagatgcggt agctttcggt
    16081 gccctcggcg agccaggcgt cgcggtcgga ggtcggcggc agctgcttgg gatagaagcc
    16141 gcccttggcg ccggtcggga cgatcaccgc attcttgacg acctgggcct tcatcaggcc
    16201 gaggatctcg gtgcggaagt cgtcgcgccg gtcggaccat ctgaggccgc cgcgggcgac
    16261 cgggccgccg cgcaggtgga tgccctcgac gcgcgggctg tagacccaga tttcgcgcca
    16321 cggcaccggc gccggaaggt tcggcacctg gcaggaatcg aacttgaagg cgagcgcctc
    16381 ccggctcgac tcggcgaagg cgttggtgcg caacgtggcc gtgacgatgc cgcggatcag
    16441 ccgcaggata cggtcctcgt cgatcgcggc gacctgggcg aggccgcggt tgattgtctc
    16501 ttcggccgcc cgcaccgcgt cggcgcgcct ggattcgagc gagggatcgt gctgggcgtc
    16561 gaacagggcg atcagcccgc gcgcgaccgc cggggcgtgg cggagggcgt cgaccacggt
    16621 ggcgaggctg taggcgatcc cggtctggcg cagataacgg aaccaggcgc ggaacaggac
    16681 gacgtcgcgc ggcccgagcc cggcgccgac cagcaactgg ttgaagccgt cattttcggc
    16741 gcggttctcg agcacggcac cgatcgcgcg ctcgaccacc tgggcgcggc tcatgacctc
    16801 gccggcatcg ccgccgcgcg gcatttcgag cgcgaaatcg tggatatagc cgaggtcgcc
    16861 gccgtcgagc ggggtcggca gctcctcgag cacctggaag ccgaaattct cgagcaccgg
    16921 caccgcttcg gagagcggga tgataccggc caggcgatag gtcttgaggt gcagccggct
    16981 cggcccgtcg ccgacccggc ggtagagccg ggcgtcgcgg gcgctggcgc cttcgagctc
    17041 gcagatgcgc agcacgtcct gggccgcttc gtcggggcct gtgcggaccc ggtagctttc
    17101 ggggaagtcg tcgacgaagc cgatcgccag ccgggtggcg cgggccggac cgaccagcgc
    17161 gccgagcgct tcctcgacgc tcggttccca gccccgcacc atggcgtcga gccgctcgtc
    17221 gagcacgtcg acatcgggca aaggcgtcgc cgggctgacg ctgagcgtgt agcggatgag
    17281 cgcgaggtcc ccgtcgccga gctcgaccga ccagctcgtg atccgcccct gcgcggcttc
    17341 ctcgatcatg gcgccgatgg cgacgcggcg gcgggtggtg agctcgtcgc ggggcagcca
    17401 cacgaaggcg aagacatggc ccttgaggct gctgcggagc agcaccaaag tcggccgcgg
    17461 ccggtccgcc agcgacatgg cggtcatcac cagctcgcgc accgcatcgg ggtcgaggtt
    17521 gacgagcagg tcgcgcggca gcgaggcgac ggcgtggcgc agcgccttgc cggcatggcc
    17581 gttgggatcg aagccgaatt cgcggtcgag ctcacccagc cggcggcgga tcaccggcac
    17641 gtcttcggcc ggggcggtca gcgcctggct ggtccacagc ccggcatgga cgccgacgcc
    17701 ggtgatcctg ccattctcgc ggaccggcac gacgacaagg tcgaacggca cccggcgatg
    17761 gacggtcgac atgcggtcgg ccttggcgac gagcggctcg gccccgccat tttcgaacca
    17821 ggcgatggcg ccgtcgcagc cgccctcgtc ggtcgggtcg ccgggaatcc tgaacagccc
    17881 gagcccctcc gaaggcacct cgcccggccg ctcgacatga tagccgagca aggtcatggc
    17941 gccgtcggcg aaccagtgga gcaaagccgc accctcctcg tcgcggaggg tttcggcgtc
    18001 ggcatgcatc ttggcctgga gcgcgcgcca gtcggtgacc gagccgcgga cgtcggcgag
    18061 gacgcggtgg agctcgccga ccagttcgtg gcggccgcgc gcgtcggtgc ggtcggcctc
    18121 gatatacatg atcgattcgc gcttggagag atcgtcgcag agcgggccga ccgaaagcag
    18181 acagccctgc gcgtcgcgct caacgcacaa gaccgggtgg agcaggcggt ggatggtgat
    18241 gccgcgcgcg gcgatcgcgc cggcgatcga atcgaccagg aagggcatgt cgtcgttgac
    18301 gatgccgatc cgcatccggc gcttgccgag ctggacgccc atcgattcca gccggaccag
    18361 cgcgatgccc ggcgggcggc gctgggcgca ggcggcgacg aagcgcgccg cctccaggcg
    18421 gtcctcgtcg ctgaagccgg caatctcgtc cttgagcgcg ccccggacca gggcgtcgcg
    18481 gagcgcgggg atgtagggca ggtcggcggc tgcggcggcg cccgctggct tgtcgctgac
    18541 ggcgcttccg gcggaaggcg ctcccgatcc agcaacaatg tcggcgttca tagggtaggc
    18601 tcctggcgcg gacggcagga cggcgccgtc ggcttggtcg cgggcgctat atgcgcccta
    18661 agggggcagg ggcaaggccc ggccggactt gggaaaagcc gatgccggcg aagctccggc
    18721 ggcgggcggg gggatcaggc ggcggggcgg ccggccacgg ccttgcggag cagcgcctgg
    18781 gcggatttgt agccgacccg ctgcccctcg gacagcatgg caaggtagcg gcgcttcagc
    18841 cccgcctccg ttcggggcga ctcggcgtcg gggagcgccc gtgcgatcgt gtcggttatt
    18901 tccggctgga gatattcccg gccgatgatc ccgccgaacg actcggcgac cggatcgtaa
    18961 cgctggcggc gcagcatgaa gtcgacgagg cgctggccga tccccagctc ggcggccacg
    19021 acccaggccg ctttcagcag cctccgtgac gcccagctgt cgttgctcag ccccgccgcc
    19081 tgcgacaagg cgaatatccg gtcctcgatc gcgggcgcct cgaccagcgc gccccgacga
    19141 agcgcaccgg tgaactgccg gtggagcgcc atgaattccg gcagatagcg ccgggcgacc
    19201 ttcggcttgt aacggtcggc gacctgctgc gcgacgtagc gggcgcgcag atcgagcaag
    19261 gcgatcagcc cgtcggtatg gccgtcgaga gggtcttcca tcgcgccgcc ttagcgccgg
    19321 cgccgccaac ggcgggttaa gaggatcgcg ggctccggcg aggtccggtc gaaagcggac
    19381 cgacgccggg gcggtacgga ggcggggctt taccggtcgg caacccaatg gtgcgatcct
    19441 gccgcgatgg gtcggaagaa atctcacccc aagtggttcc tgcccgggtg ggtgcacagg
    19501 cgtttgcgcc agcgcgaaaa gttcgcgcag gtgagggcgc tgtggggcga cgattgctgg
    19561 cgctgcaaca agccgatgcg cttcggcggc ctgccgaatt gcggcaaggc ggccacgatc
    19621 gaacatctga cgccgctctc gaagggcggc acctgggcgc tcgacaatct ccggctctgc
    19681 catgtcggct gcaatcgcca tcttggcgac catccgcgcg agcataagga gcggatgcgc
    19741 atcaaccttc gtccggcgca gggcagaccg tcatgagcaa gcccgcccaa gcagccggat
    19801 ggctcgccct gctgctgctc ctcgcaaccg cacccgcgga cgagccgcgc ccgggcaccg
    19861 tcttcgagcg gaggacgaac gcctcccagc cggttctcgt ctccggctgg cgccatctgg
    19921 gcggcggcta ttggggcgac gccaaccggg atgaatgctg cttcgacctg ttccggtccg
    19981 gcagcgcttc gatcctggcg attgtggagc cggtcgcccg cgacgcccgc ggcgggatgc
    20041 tgtcgagccg catcctcgag acgcaccgga tgaagcttga gccgaacgag gattgggcca
    20101 cctgctcgct cgccggccgg gcggcgctgc tcgcggcgct ggacgcgaag accggccgcg
    20161 tccggggcgt gttcatgggg agggacggct tcgaagccgc cacctgggcg gcccgggcgc
    20221 cggcgccgga ggagacgacc tactgcgagg aaggcggctg aagccccgtt gcagccggcg
    20281 gcgtacccgg cctggccgcg accccggccg cgcctagaag cgcggccggg gcgtgcggtc
    20341 cttgttgcgc ttgtcggaga catagctgtg gccggtctcc tccgccttga cgatatcgaa
    20401 caggccgacc gcctcgaaca gcttcgggaa attgcgggcg ccgtaattgc gcgggtcgat
    20461 cggcgcctgg cggcgggccg cgctgccggc ggcgtcgagc ggcgcccagc catcgtcgcc
    20521 ggcggtcgct tcgacggcgc cgcgcagcac cgcgaccagc ctggcgtcct gcgcgagcgg
    20581 cggcttcgac gccgcatcgg cgggcttggc cgcgggcgcg ggcgccggct tggccgcagg
    20641 cgcgggcgcg gatttggacg aagacgccgg ggcgggcctg gccgccggcg cgggcctcgg
    20701 ctccctggcg ggagcggccg cggcgggggc ctcctcgacc aggttctcga gatagaggaa
    20761 ggtcgagcag gcgttgacga acggatcggg cgtcttgcgc gcgccgaagc catagacttc
    20821 atggccgttg gcgcgaagct gcatgacgag cggcgtgaaa tcggcgtcgc tcgaggcgat
    20881 gcagaaagcg tcgggctttt gcgtgtagag cagctccatg gcgtcgatca ccagcgccat
    20941 gtcggtcgcg ttcttgccct tggaatagct gaattgctgg atcgggcgaa tggcatgctc
    21001 gtgcagccgc gcggtccagc ccttcatcga cgggctcgcc cagtcgccat aggcgcggcg
    21061 gatgttggcg acgccatatt tggacagctc ggccagcatc gccgcgatct tgccgtgcga
    21121 cacgttgtcg gcatcgatca gcaaggcgat gaggcggtcg cgggacgaat tgccggactg
    21181 gtctcgattc atgcgggtct ccattccgcc tacgcctaca ggcgcggcgg gcgggggtcg
    21241 agttggcatc gctcgcccgg gccttcggcc gaggctttac tccggcgccg atcgccgcta
    21301 tcggcccgct cccgcgcaac gcggcgcgat cgaagaggag gcgacagtga gacataaccg
    21361 caacgacgac ctcccatgct ttcgaggtca cctccaagcg tcgtaaaggc ttcccgtccg
    21421 agacgcgggt gaagcggggt ctccgcttcg tgcacggcgg cgagaaggag ctggtcgaaa
    21481 agctcggccg cgccgaccct tgcccgtgcg gctccggaaa gagctttcaa gcgctgctgc
    21541 cttagcaccg gccggtttcg acggggcgga gcgcaaggat tattggcgcg actgaggctc
    21601 ctgcccgcat agtcgcggag gcaagccccc catccccaac cctcgccgcg ccgggttcag
    21661 ccctcggtga cgcggccgct tatctcgccg gggttgaagg cgtcggtgtg gacgttgagg
    21721 atgacgaggc cgccgcgcat cgccgccacg aactggtcga aggtcagcgt cgatttcagc
    21781 agcgcggcgc cggcggaata gtcgtaatcc ttcatggcca cgcgcatgcc gctcttggtc
    21841 gaggtgtagc ccgggccgaa agccaccggc aggaccagca ccgaatcgcc gccggccggg
    21901 gtcgcatatt tgtggaagtg gatcggcccg atcggccccg cgaccaattt gtcccacaag
    21961 gcctcggtgg tgatgccgtc gacaatcatg tcgaccgaga cgcgccggct ctccgtgtcg
    22021 accttgatcc gggcgctgcc ggtcgcggcg gagccggtcg gcgcgtcgcc atatttgccg
    22081 tcgagcttgg cctggaaggt taggatctcg gcctgcgccg gcgcggcggc gagggcggcc
    22141 gcgagcgcga tcagagtggc cgcgatcggt ttggaaatag acatccccgc ttcccctttg
    22201 ccgttcaggg gaggatgggc gcgatgcggg gcgaagtaaa tgggaggcgg cggcgggcac
    22261 ctcgcccccc gattgccgtg atcggccggg ggccgtggcg gcggcatcgc gttcagttcc
    22321 cgcgcaacgg gtccggcgac ccttggccgc ggcttttccg cttgctgatc gcttcctgca
    22381 tcagctggtt ccagggcttg ttggggccgg cgagctggcg gtcgatatcg tcggcgacga
    22441 cacgggattc ctgatcggcg gcagcgcgaa acctgtcgcg ctcggcgacc agcatcgcct
    22501 tttgccgatc ggccgggagc gcggccgccg cgatgtaggt gcgggtcgcg cggacccagg
    22561 gcgagccggc cgacaggaca tcctcggccc atcgcgaaaa ggcgcttccg gcgggcacca
    22621 gcatgcctcg gatcggggcc ggcacttgcc tgcccttcat ggctttttcg atcgggttca
    22681 agaagaacag gacggtggcg ccgcgaacga acatgaagcg cgtgcagctg cctatgtaag
    22741 cgagcgggtg ggcctgctcg agctccagcg ggctgctgag cagggcgaag cgcggctccg
    22801 cgatcgcgcc gggcaaggtc agcggcttgt ccatggggag ccggcccttg agcagctcga
    22861 tcggggtgac ggtcatcgtg atgttcggat gggcggtgcc ggtggtgccg gattcgaccc
    22921 gcgcgagcag gatgatttcg gcgtcggcgg cgagctcgac attggtcggc acccgatagc
    22981 tgtcggcgac gctgcacgcc tgggccgggg cggccagcag ctggaacaaa gcggcggcgg
    23041 cggccagcag gcaaaggcgg gggaagggcg gggtcctgcg catcgggtgg atgctagcag
    23101 gcgcgcggcg cggttgccat ggcggcggcg gctgtccgaa atcggacgaa gttcaggaaa
    23161 gtcagcgggg ttcgtgcgcg cgcgaggtca gccatggccg ggctccggcg gctgcggtgg
    23221 gttcggcggg ttcgtcggcg ggtccggccg gagccctttc gcctccgctt cgtagccctc
    23281 ctccggcggc aggtcgcgca gattgtcgag aaacagatcg aacaggccgg cggtctcggc
    23341 gagcgggtcg agcggcaggc cgtcgcggtc ggtcgcggcc ggcgcggcgg cgggcgggcg
    23401 gccgaagcga accggatcga gccgcgacag caggaagatc agcgcctttt cggaggggcg
    23461 ccgccgctgg tagacgattt cgccgtcgcg ccagacggtt tcggtcatcc cgtcaagcgc
    23521 atattcgtag agcttggccg ccgcgggccg cgccgccatg cgcaaagccg cgtcccaggc
    23581 gttgcagaat tgctgcgcat cgggccgctg cgcgagccgg tgcgccgagc tctcgctcat
    23641 ccccgccgcc gccgcggcga accgcaccac gccggtctcg gcgagcacgc cgatgaaccg
    23701 ccgctgccgc tccgcggtcc agccgtcgcg gcgcggctgg accgggaccg gatcgaaggg
    23761 gatggcggcg cggggggtgc cggcggaggg gtcgttcggc ggcggatcgc cggatggcgg
    23821 cgggaggggg atatccatgg ctcggttcct ttcgaatcgg ttagggaaca gagccggaac
    23881 aaatatgatg agaaaggggc tgtaggaaaa tggttttttt ggggggggcg gtggcggcga
    23941 attagccagc cgtgatcctc gtcacggcaa ccccggcctc ttggtgccaa gaaaggggct
    24001 cggccggcgg cggcgtctgt gggcgatcgc gcgtccggtg caactcagac agtggagcga
    24061 gccatgacgg accaacggca atcggatgac ggcaaagagg gcatcccctg ccccttcggc
    24121 gatggcggcg cgcgcaactg gaaggcctgg gtcaacgcca tgcccggcgc ggacgcgcag
    24181 ctgatcgtga tcggcgaggt gaatgcgccg caaggctata ccggccgcct tcgctttggg
    24241 atcgccgaca agaggaagcc gccgacgcag tatctcgaac tcaacctggt ggaagaggcc
    24301 ggcgcgccgg acggctggcg tgagatccgc gcgcagggca aggcgctggg gcgcgaatat
    24361 gcggcggtcg tgatcctgtg ccacggcgac gagctcgcgc ggatcaccga ggtgccggtg
    24421 gtggagtaag gggcgcggcg gctttcggc
//
LOCUS       DASVRK010000255         4837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_486254, whole genome shotgun sequence.
ACCESSION   DASVRK010000255 DASVRK010000000
VERSION     DASVRK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486254"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VF589_10140"
     CDS             complement(<1..1192)
                     /locus_tag="VF589_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8257984.1"
                     /translation="MKNKLFAAALLLAAAFPAAPAQADALSEAVSKDMPSLLALYRDL
                     HANPELSMQEVRTSKKLAAEAKKLGFKVTTGVGKTGVVAVMENGPGPVVLLRADMDGL
                     PVVEQTGLPYASKVKGTSRAGGETGIMHACGHDTHMTAWVGAARRLAATKDQWSGTLV
                     MILQPGEETGEGAQAMLADGLYTRFPKPASVIAFHDAAALPAGVIGYSNGYALANVDS
                     VDILVKGVGGHGAYPHTTKDPIVLASRIVSALQTLVSRENDPQSPAVVTVGSFHAGAK
                     HNIISDEATLLLTVRSYKDETRKLLLDGIKRVVRGEAIAAGMPEDRMPIVTIREAEYT
                     PATFNTEKLTTSTAALFTQRFGAERDKETPAVMGGEDFSRFYLADKTIQSLIFWVGGV
                     PKAQW"
     gene            complement(1210..1860)
                     /locus_tag="VF589_10145"
     CDS             complement(1210..1860)
                     /locus_tag="VF589_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEX8257985.1"
                     /translation="MPTIKTIAWIAAAGAALLAGSGSAAQPDCPPGARIPAAANWRTL
                     ATANDRARLREWREAWVEGLEKAAVAGHAATIGGEGALLDPDAAVAFAPPLPGAYRCR
                     TIKLGGQSEGLLDYVAYPWFECRVGAAANGALRFEKVSGSQRPAGRLLADSDRRMIFL
                     GSLVLGDEQRPLDYGHDRERDLAGILERIGDTRWRIAFPRPAFESTIDVIELVPAS"
     gene            1975..3534
                     /locus_tag="VF589_10150"
     CDS             1975..3534
                     /locus_tag="VF589_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257986.1"
                     /translation="MTVRHYLAGALLALGIAPQAVAATKAVIPNESQRAAAVAKAEAA
                     IAGIDQERLLADRDYAGEMLAHVETLRTVRTEPDALFALDTLRLVALLGAVRNADAAE
                     LGVKLIAQRPSEPMVYPLALWAAVLAGKSDAAVPMLESAARHVTRPEPLEEVRSALDQ
                     DLLDGIIRPLAQARNDRATQRLAEALLIIGSPVFDNAVEADRYRVLAIDGRLAAGDVA
                     GARALADRVIETGATLRLIGAHRYDPLFAGDADRLARLDRATAAYDADTKKRAEMKAD
                     DLGARLFRGQFLRGVGKEQEALAVLLPATRDMAAVEAGGEMAFWIVNEAAYALTSLGR
                     NAEAIALMERLIALDMKKHPYLISMAINHGELLNSAGRHREAAAYETMLMKKGDGFDS
                     PYGRMWMWSAAACGHALAGHPAAAAPWLEKLEKRSSDNEAAHMRALLCANQLDRAEQL
                     MLTRLGGRHAAELLLRMHDYQLGSFKSPARRLLQERGLAVARRSAVRAAAAKVGRTLS
                     LPLSKVFWGDY"
     gene            complement(3540..4295)
                     /locus_tag="VF589_10155"
     CDS             complement(3540..4295)
                     /locus_tag="VF589_10155"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX8257987.1"
                     /translation="MFLKGKTALVTGSTSGIGLAYARALAAEGAKVTLNGFGDPGEIA
                     KLKEELGAGYSNADLTKPEAIEAMMAEAGPVDILVNNAGMQHVAPIDEFPVAKWDLLI
                     ALNLSAAFHTIRLALPGMKAKGWGRVINTASAHSLVASANKAPYVATKHAIAGLTKAV
                     ALEMATAGVTVNCISPGYVWTPLVENQIPDTMKARGLTREQVMNDVLLAAQPTKRFVT
                     PEEVAALALFLCRDEAGSITGANLSMDGGWTAQ"
     gene            complement(4310..>4837)
                     /locus_tag="VF589_10160"
     CDS             complement(4310..>4837)
                     /locus_tag="VF589_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HEX8257988.1"
                     /translation="PVEIDGKYWWDGGIVSNTPLAHVLDRQGCDALVFQIDLFPAAGA
                     LPKTLLDVHEREKEIRYSSRTRQISDTLLRRRQERELIRKVLAKLPARLAADSDVAAL
                     GELAHEHAVSLVQLIYRANAWEGGKRDYEFSARAMAEHWQAGRAAIAETMTRSELIAR
                     NIHDGATAAFDLTRR"
BASE COUNT          696 a         1586 c         1749 g          806 t
ORIGIN      
        1 cccattgcgc cttgggcacc ccgccaaccc aaaaaatcag actctggatc gtcttgtcgg
       61 ccaggtaaaa gcggctgaaa tcctcgccgc ccatcaccgc cggcgtctcc ttgtcccgtt
      121 ccgcgccgaa gcgctgcgtg aacaaggcgg ccgtgctggt agtgagcttt tcggtgttga
      181 aggtggccgg cgtatattcg gcctcgcgga tggtcacgat gggcatccgg tcctcgggca
      241 tgccggcggc gatcgcctcg ccgcggacca cgcgcttgat cccgtcgagc aggagcttcc
      301 tggtctcgtc cttgtagctg cggacggtca gcagcaacgt cgcttcgtcg gagatgatgt
      361 tgtgcttggc gccggcgtgg aagctgccga ccgtcaccac cgccgggctc tgcgggtcgt
      421 tctctcggct taccagcgtc tgcagcgcgg agacgatgcg gctcgccagc acaatcgggt
      481 ctttggtggt gtgcggatag gcgccgtggc cgccgacccc cttcaccagg atgtcgacgc
      541 tgtcgacatt ggccagcgca tagccgttcg aatagccgat cacgcccgcc ggcagcgccg
      601 cggcatcgtg gaaggcgatc accgaagcgg gcttgggaaa gcgggtatag agcccgtccg
      661 ccagcatcgc ctgcgcgccc tcgccggttt cctcgcccgg ctggaggatc atcaccagcg
      721 tccccgacca ttggtccttc gtcgccgcga gccggcgggc ggcgccgacc catgcggtca
      781 tatgggtgtc gtggccgcag gcatgcatga tgccggtctc gccgccggcg cggctggtgc
      841 ctttcacctt gctcgcatag ggaagcccgg tctgctcgac caccggcagc ccgtccatgt
      901 cggcgcggag cagcacgacc gggccggggc cgttctccat caccgccacc acgccggtct
      961 tgccgacccc ggtggtcact ttgaagccga gcttcttcgc ctcggcggcg agcttcttgg
     1021 aggtccgcac ctcctgcatc gacagctcgg gattggcgtg gaggtcgcgg tagagggcga
     1081 gcagggacgg catgtccttg gaaaccgcct cgctcagcgc gtcggcctgg gcgggagcgg
     1141 cggggaaggc ggcggcgagc agcagggctg cggcgaagag cttgtttttc atgagccttt
     1201 cctagcgggt caggaggcgg gaaccaactc gattacatcg atggtggatt caaaggcggg
     1261 gcgggggaag gcgatccgcc agcgcgtgtc gccgatccgc tccaggatgc cggccaggtc
     1321 gcgctcgcgg tcgtggccgt aatcgagcgg gcgctgctcg tcgccgagca ccagcgagcc
     1381 gaggaagatc atgcggcggt cggaatcggc cagcaggcgt ccggccgggc gctgcgagcc
     1441 gctcactttc tcgaagcgga gcgcgccgtt cgccgccgcg ccgacgcggc actcgaacca
     1501 cggataagcg acataatcga gcagcccctc cgattgcccg ccgagcttga tcgtgcggca
     1561 gcgataggcg ccggggagcg gcggagcgaa agcgacggcc gcgtcggggt cgagcaaggc
     1621 gccctcgccg ccgatcgtcg ccgcatggcc ggccaccgcg gccttttcca gcccctcgac
     1681 ccaggcctcg cgccattcgc gcagccgggc gcggtcgttg gccgtcgcga gcgtgcgcca
     1741 gttggcggca gcggggatcc gcgccccggg cggacaatcg ggttgcgccg cggagccgct
     1801 gcccgcgagc agcgcggcgc ccgcggccgc gatccaggcg atggtcttga tggtcggcat
     1861 gccggcggct cttagcacaa gtggagccgc tgtcgccaag tcgcccgatc gcttttccga
     1921 tgcttgtgcc ggggccgatc tccggccatt ctgaccgttc aagtgggggt tccagtgacc
     1981 gttcgacatt atctcgcagg cgctttgctg gcgctcggca tcgcgcccca ggctgtggct
     2041 gcgaccaagg ccgtcatccc gaacgagtcg cagcgggcgg cggccgtggc gaaggcggag
     2101 gcggcgattg ccggcatcga ccaggagcgc ctgctcgccg accgcgacta tgccggcgag
     2161 atgctcgctc atgtcgaaac gctgaggacg gtgcggacgg agccggatgc gctgttcgca
     2221 ctcgatacgt tgcggttggt cgccctcctg ggggccgtgc gcaatgccga tgcggcggag
     2281 ctcggcgtga agctgatcgc gcagcggccg tccgagccga tggtctatcc gctcgcgctg
     2341 tgggcggcgg tgctcgcggg caagtcggac gccgcggtgc cgatgctcga atcggccgcg
     2401 cggcacgtga cccgcccgga gccgctcgag gaggtgcggt cggccctgga ccaggatctc
     2461 ctcgacggga ttatccggcc cctggcccag gcgcgcaacg accgtgcgac gcagcggctg
     2521 gcggaggcgc tgctcatcat cggctccccc gtgttcgaca atgcggtgga ggccgaccgc
     2581 taccgggtgc tggcgatcga cggccggctg gcggcgggcg atgtcgcggg cgccagggcg
     2641 cttgccgatc gcgtgatcga aaccggcgcg acgctgaggc tgatcggcgc gcaccgttac
     2701 gacccgctgt tcgcggggga cgcggaccgg ctggcccggc tggaccgggc gaccgccgcc
     2761 tatgacgcgg atacgaaaaa gcgcgccgag atgaaggcgg acgatcttgg cgcccggctc
     2821 ttccgcggcc agttcctgcg cggcgtcggc aaggagcagg aggcgctggc cgtgctcctg
     2881 ccggccacgc gggacatggc ggccgtcgaa gcgggcggcg agatggcctt ctggatcgtc
     2941 aacgaagccg cctatgcgct gacctcattg ggccgcaacg ccgaggcgat cgccctgatg
     3001 gagaggctga tcgccctcga catgaagaag catccctatc tgatcagcat ggcgatcaac
     3061 catggcgagt tgctgaactc cgctggccgc caccgggagg cggccgctta cgagaccatg
     3121 ctcatgaaga agggcgacgg cttcgacagt ccttatggac gcatgtggat gtggtcggcc
     3181 gccgcctgcg gccatgccct ggccgggcat cccgcggcgg cggctccgtg gctggagaag
     3241 ctcgaaaagc gaagctccga caatgaggcg gcccatatgc gcgctttact ctgcgccaac
     3301 cagctcgatc gggccgagca gttgatgctg acgcggctgg gaggcaggca cgcggccgag
     3361 ctgctgctgc ggatgcacga ttatcaactg ggcagcttca agtcgcccgc ccggcggctg
     3421 ctccaggagc gggggctcgc cgttgctcgc cggtcggccg tgcgggcggc ggcggccaaa
     3481 gtcggccgga cgctgtcgct gccgctgtcc aaggttttct ggggcgatta ttgaggcggc
     3541 tattgcgccg tccagccgcc gtccatcgac aggttggcgc cggtgatcga cccggcttca
     3601 tcgcggcaca ggaacaaggc caaggcggcg acttcctccg gggtgacgaa gcgcttggtc
     3661 ggctgggcgg cgagcagcac gtcgttcatc acctgctcgc gggtgaggcc gcgcgccttc
     3721 atcgtgtcgg gtatctggtt ctcgaccaac ggcgtccaga catagcccgg gctgatgcaa
     3781 ttgacggtga ctccggccgt cgccatctcg agcgccaccg ccttggtgag gccggcaatg
     3841 gcatgcttgg tggcgacgta gggcgccttg ttggcgctgg cgacgaggct gtgcgcggag
     3901 gcggtgttga tcacccggcc ccagcccttc gccttcatcc ccggcaaagc cagccggatg
     3961 gtgtggaagg cggcgctgag gttgagcgcg atcagcaggt cccatttggc gaccgggaat
     4021 tcgtcgatcg gggcgacatg ctgcattccg gcattgttga cgaggatgtc gaccgggccg
     4081 gcctcggcca tcatcgcctc gatcgcctcg ggcttggtca ggtcggcatt gctgtagccg
     4141 gcgccgagct cctcctttag cttcgcgatc tcgcccggat cgccgaagcc gttcagggtg
     4201 accttggccc cctcggccgc cagcgcgcgg gcgtaagcga ggccgatgcc ggaggtcgag
     4261 ccggtgacga gcgcggtctt gcctttgagg aacatgggcg gtccttatgc tagcggcggg
     4321 tgaggtcgaa ggcggcggtg gcgccgtcgt ggatgttgcg ggcgatcagc tccgaccggg
     4381 tcatcgtctc ggcgatggcg gcgcggccgg cctgccaatg ctcggccatg gcgcgggcgg
     4441 aaaattcata gtcgcgcttg ccgccctccc aggcattggc gcggtagatg agctggacca
     4501 ggctcacggc atgctcgtgc gcgagctcgc cgagcgcggc cacgtcggag tccgcggcga
     4561 gcctggccgg cagcttcgcc agcaccttgc ggatcagctc gcgctcctgg cggcgccgca
     4621 gcagggtgtc ggaaatctgc cgggtgcggc tcgaatagcg tatttccttt tcgcgctcgt
     4681 ggacgtcgag gagggtcttc ggcagcgcgc ctgctgcggg gaacaggtcg atctggaaga
     4741 cgagggcgtc gcagccctgg cggtcgagca catgggcgag cggcgtgttc gagacgatgc
     4801 cgccgtccca ccaatatttg ccgtcgatct cgaccgg
//
LOCUS       DASVRK010000256         7675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_46505, whole genome shotgun sequence.
ACCESSION   DASVRK010000256 DASVRK010000000
VERSION     DASVRK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46505"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF589_10165"
     CDS             complement(<1..288)
                     /locus_tag="VF589_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX8257989.1"
                     /translation="MSFAATILTLYPEMFPGPLGHSLAGRALADGLWSIETVQIRDFA
                     ADRHRSVDDTPAGGGAGMVMRADVLAAAVDDVLERRPEAPIVAMTPRGEPLT"
     gene            complement(285..1790)
                     /locus_tag="VF589_10170"
     CDS             complement(285..1790)
                     /locus_tag="VF589_10170"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8257990.1"
                     /translation="MRLLKAWFAALLLIVPALASAQPAPPKVETAIPEIDRIFAAFQA
                     ETHAPGLVYGIVQDGRLVHVKALGVQDLKARRPVTPDSLFRIASMSKAFTALAILNLR
                     DAGKLSLDAPAETYVPEMRGWTYPTADSPKIRVRDLLNHSAGFVTDDPWGDRQQPLSE
                     AAFTRMLKAGVPFTRTPVTAFEYSNFGYALLGRIVTNVSGEPYDRYIQRTIQRPLGMA
                     STGYEVRDWPLARRALGYRWENERHSEEPTMAHGAFGAMGGVQTSANDYARWMAFLLS
                     GWPARDGAEAGPVRRATVREMAQGSNFVRVRPRFGSTGEAACRQAAAYAMGLIAAQDC
                     ELGLTLSHGGGYPGYGSHMLLLPDHNVGLFVFSNRTYNGGAGAVWDAAIAFHRAGALE
                     SRTVPVSPALARAYAAATRIWRARDVGAAADLLAMNFLMDRSRENWRAELVRLQGEVG
                     DCQTSAPIEPNGALAGRFRWTCERGAVEGQLLLAPTAPPGIQGLRLAAVAP"
     gene            complement(1790..2176)
                     /locus_tag="VF589_10175"
     CDS             complement(1790..2176)
                     /locus_tag="VF589_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8257991.1"
                     /translation="MTKPSFLLDTNILVYLVAGNSEPLRDQVEQRPPGSLVTSALCVA
                     EALYGMGESVAVRSSFDRLLQVVEPQPFGLAAARAFPSVPFRRGKLDRLIAAHTLALG
                     LTLVTNNEADFEDIPELKVENWTRRR"
     gene            complement(2169..2426)
                     /locus_tag="VF589_10180"
     CDS             complement(2169..2426)
                     /locus_tag="VF589_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX8257992.1"
                     /translation="MKTTKTFKSGNSVAVRLPKSLGVDAGVEMIVREERGRYIVEPVA
                     KPQERIDLTGIYGSCPGLKPVERRDMAPRELDWQGKLLPRD"
     gene            2530..3465
                     /locus_tag="VF589_10185"
     CDS             2530..3465
                     /locus_tag="VF589_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8257993.1"
                     /translation="MDYDCLVIGGGPAGLTAAIYLTRYHLSLKVVDSGKSRAGWIPCT
                     HNHAGFPGGISGKELLARMREQAQMYGASIETNRVGRLDRIEGGFMAEWGAGSVTAKK
                     VLLATGVTNRRPPMDEDLHNEALAQGLIRYCPVCDGYEVTDKRVGVIGSGGHGVAEAV
                     FIRGFTTDITLIAPDRAHDLSDEDRARMDEFGIKCVDGPCQAVAAAGKCITIATPYGD
                     HVFDSVYPALGSDTHIELALQVGAELHEDDTLLVDDHQRTSVPGLYAAGDVVKGLDQI
                     SHAMGEGGVAATTIRNDLAKEKPIIRERLKGNSIR"
     gene            complement(3458..3943)
                     /gene="rimM"
                     /locus_tag="VF589_10190"
     CDS             complement(3458..3943)
                     /gene="rimM"
                     /locus_tag="VF589_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456920.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX8257994.1"
                     /translation="MAEPQVTLAAIAGAHGINGEVRLKLFAETVESLSRYETVQVGER
                     RLTLKSVKAGGGAPIARFAEIADRNAAEALRGSLVMVPRSALPPLEEGEYYHADLIGL
                     PCESAAGEPLGTVVAVENFGAGDILEIERPDGRRVMVPFRAGVADLAGAAIQVDPAFL
                     T"
     gene            complement(4207..4674)
                     /gene="rpsP"
                     /locus_tag="VF589_10195"
                     /pseudo
     CDS             complement(4207..4674)
                     /gene="rpsP"
                     /locus_tag="VF589_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(4754..6247)
                     /gene="ffh"
                     /locus_tag="VF589_10200"
     CDS             complement(4754..6247)
                     /gene="ffh"
                     /locus_tag="VF589_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX8257995.1"
                     /translation="MFDSLSDRLSGVFDRLRGRGALNEADVRAAMREVRIALLEADVA
                     LPVVREFVERATEKAVGHQVLKSVTPGQQVVKIVHDALVEMLGADASELDIEVTPPAI
                     VMMVGLQGSGKTTTTAKIAKRLSEKGRKKVMMASLDVARPAAQEQLAVLGTQAKVATL
                     PIVPGQQPVEIARRGLQSARLQGYDVLLLDTAGRLHVDQALMDEMKAVAEVSQPHEIL
                     LVVDALTGQDAVNVAQSFAGQVDLTGVVLTRMDGDARGGAALSMRAVTGKPIKFAGVG
                     EGIDALEGFHPSRVAGRILGMGDVVSLVERASETIQIEEAEALARKMAKGQFDLNDLR
                     SQLMQMQRMGGLGALAGMLPGMKKMAGAASAADEKTLVRLDAMIGSMTPKERAKPELL
                     NAKRKIRVAKGSGTTVQEVNRLLKMHQEMANAMKKIRKMGGLGKLGALLGGGGGGLGG
                     LLGGGMGGMGGMGGPGAGPPAGLPGLPGGPGAQPFNLPPGFDKFMKK"
     gene            6467..>7675
                     /locus_tag="VF589_10205"
     CDS             6467..>7675
                     /locus_tag="VF589_10205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8257996.1"
                     /translation="MASLFKKERPRGGSAESSDFIIISIAVSTLLALAAVGNLYFESL
                     RQQLVRIGADNSNLVFGLLLLNVALSIYGFRRYSDVRREVVERTAAEKRAHSLASTDP
                     LTGFLNRRTLGHAVDELLASCEQRKRSVAMLIIDLDGFKTINDVHGHLTGDTLLRTTA
                     EVIEKALPPGAIGARLGGDEFAAAFAIDTDSPGTVDATAAYIVERLGKPFDLAGVHVH
                     ISASVGIALSDRDCTSVDGLLRRADIAMYAAKKHGRSRYIWFDASMERELHKRTAIEV
                     GLRSGIPQGEFVPYFEQQVDLVSGSLHGFEVLARWHHPEQGIVMPDEFIRVAEESGLI
                     GDLSFSVMRQAFQEARNWDSSLTLSVNISPTQLKDPWLSQKIVKLLVETGFPAERLEV
                     EITESSLFENL"
BASE COUNT         1184 a         2695 c         2571 g         1225 t
ORIGIN      
        1 cgtcaacggc tctccccgag gagtcatcgc cacgattggt gcctctggcc gacgctccag
       61 cacatcgtcc accgcagcgg cgagcacgtc cgcccgcatc accattcctg ccccgccccc
      121 ggccggagtg tcgtcgaccg agcggtgccg gtcggccgcg aagtcgcgaa tctggaccgt
      181 ctcgatcgac cacagcccgt cggccagcgc gcgcccggcg aggctgtggc cgagcggacc
      241 cgggaacatc tcggggtaga gcgtcaggat ggtggcggcg aagctcaagg agcgaccgcc
      301 gccagccgca gcccctggat gcccggggga gcggtgggcg caagcagcaa ctggccctcg
      361 acggctccgc gctcgcaggt ccagcggaag cggccggcaa gcgcgccgtt cggctcgatc
      421 ggggccgagg tctggcaatc gcccacctct ccctggagcc ggaccagctc ggcgcgccag
      481 ttctcgcggg agcggtccat gaggaagttc atcgccagga gatcggccgc cgcgccgacg
      541 tcgcgcgccc tccagatccg cgtggccgcc gcataggcgc gggccagggc tgggcttacc
      601 ggaacggtgc ggctttcgag cgcgccggcg cgatggaacg cgatggccgc gtcccacacg
      661 gcgccggcgc cgccattata ggtgcggttc gagaagacga agagcccgac attgtgatcg
      721 gggagcagca gcatgtgcga gccgtagccg ggatagccgc cgccatggct aagcgtcagg
      781 ccgagctcac aatcctgggc ggcgatgagg cccatcgcat aggcggccgc ctgccggcac
      841 gccgcctcgc cggtgctgcc gaagcgcggg cggacccgga cgaaattcga cccctgcgcc
      901 atttcgcgga cggtcgcgcg gcgcaccggc cccgcctcgg cgccgtcgcg cgcgggccat
      961 ccggaaagca ggaacgccat ccagcgcgca tagtcgttgg cgctggtctg gacgccgccc
     1021 atcgcgccga acgcgccgtg cgccatggtc ggctcttcgc tgtggcgctc attctcccag
     1081 cgatagccga gcgcccgccg cgcgagcggc cagtcgcgca cttcgtagcc ggtcgaggcc
     1141 atgccgagcg ggcgctggat ggtgcgctgg atgtagcggt catagggctc gcccgacacg
     1201 ttggtgacga tgcggccaag caaggcataa ccgaagttgg aatattcgaa cgcggttacc
     1261 ggcgtgcggg tgaagggcac gcccgccttg agcatgcgcg tgaaggcggc ttcggacagt
     1321 ggctgctggc ggtcgcccca agggtcgtcg gtgacgaagc cggcgctatg attgaggagg
     1381 tcgcggacgc ggattttcgg cgagtcggcg gtcggatagg tccagccgcg catttccggg
     1441 acgtaggtct cggccggggc gtcgagcgaa agcttgccag cgtcgcgcag gttgaggatg
     1501 gcgagcgcgg tgaaggcctt cgacatggag gcgatgcgga acaggctgtc cggcgtcacc
     1561 ggccgccgcg ccttcagatc ctggacgccg agcgccttca catggaccag ccgcccatcc
     1621 tggacgatgc cgtagactag ccccggcgcg tgcgtctccg cctgaaaggc agcgaagatg
     1681 cggtcgatct ccgggatggc ggtctcgacc ttcggcggcg cgggctgcgc cgaagccagc
     1741 gcagggacga tcagcagcag cgccgcaaac caggctttca gcaatctcat taccgcctcc
     1801 tggtccaatt ctcgaccttt agctccggta tgtcctcgaa gtccgcttcg ttgttggtga
     1861 cgagcgtgag ccccagtgcc aaggtgtggg ccgcaataag ccggtcgagc ttccctcgcc
     1921 gaaacggcac ggatgggaac gcccgcgccg cagcaaggcc gaatggctgc ggttccacca
     1981 cctggagcag acggtcgaag ctgcttctta ccgcaaccga ctctcccatc ccgtacaagg
     2041 cttccgcgac gcagagggcc gaggtgacga gactgccggg gggccgttgc tccacctgat
     2101 cgcgcaacgg ttcggagttg cccgcgacca gatataccag aatgttcgtg tcgagcagga
     2161 acgaaggctt agtcacgcgg cagcagcttt ccttgccagt cgagttcgcg cggcgccatg
     2221 tcccgcctct cgacgggctt aaggccgggg cacgacccgt aaatgccggt gaggtcgatg
     2281 cgctcctggg gcttcgcgac gggctcgacg atgtagcggc cgcgttcctc gcgcacgatc
     2341 atctcgacgc ccgcatccac gccgagcgac ttggggaggc ggaccgcgac cgaattgccc
     2401 gacttgaacg tcttggtcgt cttcaccctc acctccgtat atacggcatg tatatacctg
     2461 cctccccgcc aaaattcaat ccgcgccgga acccgccccg cgccggatcg ctcagcgcgc
     2521 atggacgaaa tggactatga ttgcctggtc atcggcggtg ggccggcggg gcttaccgcc
     2581 gccatctacc tcacccgcta ccatctctcg ctgaaggtgg tggattcggg caagagccgc
     2641 gccggctgga tcccgtgcac ccataatcat gccggcttcc ccggcggcat ttccggcaag
     2701 gaactgctcg ccaggatgcg cgagcaggcg cagatgtacg gcgcctccat cgaaaccaac
     2761 cgggtcggcc ggctcgaccg gatcgagggc ggcttcatgg ccgaatgggg cgcgggatcg
     2821 gtgaccgcga aaaaggtgct gctcgccacc ggcgtcacca accgccgccc gccgatggac
     2881 gaggacctcc ataacgaggc gctggcccag gggctgatcc gctattgccc ggtctgcgac
     2941 ggctatgaag tcaccgacaa aagggtcggc gtgatcggca gcggcgggca tggcgtggcc
     3001 gaggccgtgt tcatccgcgg gttcacgacc gacatcacgc tgatcgcgcc agaccgcgcg
     3061 cacgatctca gcgacgagga ccgggcgcgg atggacgagt tcgggatcaa gtgcgtcgac
     3121 ggcccgtgcc aggcggtcgc cgcggccgga aagtgcatca ccatcgccac gccctatggc
     3181 gaccatgtgt tcgacagcgt ctatccggcg ctcggctcgg acactcatat cgagctggcc
     3241 ctgcaggtcg gcgctgagct tcacgaggac gacactttgc tggtcgacga ccatcagcgg
     3301 accagcgtgc cagggctcta cgcggccggc gacgtggtga aggggctcga ccagatcagc
     3361 cacgccatgg gcgagggcgg cgtcgcggcg accacgatcc gcaacgacct cgccaaggag
     3421 aagccaatca tccgcgagcg gctcaagggc aattcgatca ggtaaggaaa gcggggtcga
     3481 cctggatcgc ggcgccggcg aggtcggcga cgccggcccg gaacggcacc atcacccgcc
     3541 ggccgtcggg ccgctcgatc tcaagaatgt cgccggcgcc gaaattctcg accgccacga
     3601 cggtgccgag cggctcgccg gccgcgctct cgcaagggag gccgatcagg tcggcatgat
     3661 aatattcgcc ttcctcgagc ggcggcagcg ccgagcgcgg caccatcacg aggctaccgc
     3721 gaagcgcctc ggccgcgttg cggtcggcaa tctcggcgaa gcgggcgatc ggagccccgc
     3781 cgcccgcctt caccgactta agcgtcagcc ggcgctctcc cacctggacg gtctcatacc
     3841 ggctcaggct ctcgacggtc tcggcgaaca gtttcagccg gacctcgccg ttaatgccat
     3901 gggctccggc aatggcggcg agggtgacct gcggctcggc caagcttatt cggccttgtc
     3961 ggcgccttcc gccggggcgg cttcctcggc gccctcggcg ggctggtcgg tcgactcgcc
     4021 ctggttggga gcctcgtcgg ccggcgtcgc ctcgccctcg tcgccgccgg cggcggtcgg
     4081 ggcctcgtcg gcgggagcag cggcttcgtc agcagccgga gcctcgtcgg cgggagcggc
     4141 ggcttcctcg gcggccggag cctcctcagc gggagcggcg gcttcctcag ccggcgcagc
     4201 ctcgtcggca gcgggagcct cgtcggcagc gggagccgct tcggccggag ccgcttcagc
     4261 agccggagct tcctcggcag ccggggcttc ctcggcggcg ggagcttccg cagccgcggc
     4321 ggcggcttca gcggcctcgg cgtcgcgcgc ggcacgctcc tcggcgcgct ccttcgcctt
     4381 ttcgccgggc tcgcccttct tgaggttgga gcgggccggg cgctcgagca ggccggcctg
     4441 gtcgaggaag cggtggaccc ggtccgacgg ctgcgcgccg accgagagcc agtggcgggc
     4501 gcgttcggtg tcgagcttca cgcgctcttc actgtccttg gggagcagcg gattgtagct
     4561 gccgatccgc tcgatgaact tgccgtcgcg ggcattgcgc gcatcggtga cgacgatctt
     4621 gtaataaggc cgcttcttcg cgccgccgcg agcgagccgg attgctactg ccatggtatt
     4681 ttcctttctt cgttccccgg caaaggccgg ggtccagttg ttctgcttcg ggtccggctt
     4741 ccgccggggt cactcacttc ttcatgaact tgtcgaaacc gggcgggaga ttgaacggct
     4801 gagcccccgg cccgccggga aggccgggga gacccgccgg gggtccggcg ccggggccgc
     4861 ccatgccgcc catgccgccc atgccgccgc cgagcagccc gccgaggccg ccgccgcccc
     4921 cgccgagcag ggcgccgagc ttgccgaggc cgcccatctt cctgatcttc ttcatcgcat
     4981 tggccatttc ctggtgcatc ttgagcagcc ggttgacctc ctgcaccgtc gttcccgagc
     5041 ccttggcgac ccggatcttg cgcttggcgt tgagcagttc gggcttcgcc cgctccttcg
     5101 gagtcatcga gccgatcatt gcgtcgagcc ggaccaaagt cttctcgtcg gccgcgctgg
     5161 cggcgccggc catcttcttc atccccggca gcatgccggc gagcgcgccg aggccgccca
     5221 tgcgctgcat ctgcatcagc tgactgcgca ggtcgttgag atcgaactgc cccttcgcca
     5281 tcttcctggc gagggcttcg gcctcctcga tctggatcgt ctcggacgcg cgctcgacca
     5341 ggctgaccac gtcgcccatg ccgaggatgc ggccggcgac ccggctcggg tggaagcctt
     5401 cgagcgcgtc gatcccctcg ccgacgccgg cgaacttgat cggcttgccg gtgacggcgc
     5461 gcatcgacaa ggccgcgccg ccgcgcgcgt cgccgtccat gcgggtaagg acgacgccgg
     5521 taagatcgac ctggccggcg aagctctggg cgacgttgac ggcgtcctgc ccggtcagcg
     5581 cgtcgaccac gagcaatatc tcgtgcggct gcgagacttc ggccacggcc ttcatctcgt
     5641 ccatcagcgc ctgatcgacg tggagccggc cggcggtgtc gagcagcagc acgtcatagc
     5701 cctgcagccg cgccgactgg aggccgcgcc gggcgatctc gaccggctgc tggccgggga
     5761 cgatcggcag cgtcgcgacc ttggcctggg tgccgagcac cgccaattgc tcctgcgccg
     5821 ccgggcgggc gacgtcgagc gaggccatca tcaccttctt gcggcctttt tcgctgaggc
     5881 gcttggcgat cttggcggtg gtggtggtct tgcccgaccc ctggaggccg accatcatca
     5941 cgatggcggg cggagtcact tcgatgtcga gctcggacgc gtcggcgccg agcatctcga
     6001 ccagcgcatc gtggacgatc ttgacgacct gctggccggg cgtgaccgac ttcaggacct
     6061 ggtggccgac cgccttttcg gtcgcccgct cgacgaactc gcgcaccacc ggcagggcaa
     6121 cgtcggcttc gagcagagcg atccggacct cgcgcatcgc cgcgcgcacg tcggcctcat
     6181 tgagcgcgcc gcggccgcgc agccggtcga agacgccgct cagacgatcg ctcaagctgt
     6241 cgaacattcc gtcttccctg cctctacgtc caaacgcaaa agcgccggcg ggcgaaacct
     6301 cgccggccag cgcgcgccct cggacgctgc tcaaaaccca tcactgggat ggccggcaca
     6361 tagcgcccgc ccccgcgatt ggcaagccgg agcgccttaa cggcatcttc acgcccgctc
     6421 acttagatca aggctccggt ccggcgcgcg gagattgacg gcagcgatgg cgagtttgtt
     6481 caagaaggag aggcctcgcg gcgggtccgc ggagagcagc gacttcatca tcatcagcat
     6541 cgccgtcagc accctcctgg cgctggccgc agtcggcaac ctctatttcg aatcgctccg
     6601 gcagcagctc gtccggatcg gcgccgacaa cagcaatctg gtgttcgggc tgctgctcct
     6661 caacgtcgcg ctcagcatct acggcttccg ccgctattcc gacgtccggc gcgaggtggt
     6721 cgagcgcacc gccgccgaaa agcgcgccca ttcgctagct tcgaccgacc cgctgaccgg
     6781 cttcctcaac cgccgcacgc tcggccacgc cgtcgacgag ctgctcgcga gctgcgagca
     6841 gcgcaagcgc tcggtcgcga tgctgatcat cgacctcgac ggcttcaaga ccatcaacga
     6901 cgtccacggc cacctcaccg gcgacacctt gctgcggacc accgccgagg tgatcgaaaa
     6961 ggcgctgccc cccggcgcga tcggcgcgcg cctcggcggc gacgaattcg ccgccgcctt
     7021 cgccatcgat accgactcgc ccggcaccgt cgatgccacc gccgcctata tcgtcgagcg
     7081 gctcggcaag ccgttcgacc tggccggagt ccacgtccat atcagcgcct cggtcggcat
     7141 cgcgctttcg gaccgcgact gcaccagtgt cgacgggctg ctgcgccgcg ccgacatcgc
     7201 catgtatgcg gccaagaagc atggccgcag ccgctacatc tggttcgacg cgagcatgga
     7261 gcgcgagctc cacaagcgca ccgcgatcga ggtcggcctg cgtagcggca tcccgcaggg
     7321 cgagttcgtc ccctatttcg agcagcaggt cgacctcgtc agcggcagcc tccacggctt
     7381 cgaagtgctc gcccgctggc accatcccga gcagggcatc gtcatgcccg acgagttcat
     7441 ccgcgtcgcc gaggaatcgg gcctgatcgg cgacctttcc ttttccgtga tgcggcaggc
     7501 cttccaggag gcacgcaatt gggactcttc gctgacgctg tcggtcaaca tctcgccgac
     7561 acagctcaag gatccgtggc tgtcgcagaa gatcgtcaag ttgctggtcg aaaccggctt
     7621 cccggccgag cgcctcgaag tcgagattac cgaaagctcg ctgttcgaaa atctc
//
LOCUS       DASVRK010000257         5243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_486776, whole genome shotgun sequence.
ACCESSION   DASVRK010000257 DASVRK010000000
VERSION     DASVRK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486776"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(515..591)
                     /locus_tag="VF589_10210"
     tRNA            complement(515..591)
                     /locus_tag="VF589_10210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(555..557),aa:Arg,seq:ccg)
     gene            complement(638..1327)
                     /gene="nth"
                     /locus_tag="VF589_10215"
     CDS             complement(638..1327)
                     /gene="nth"
                     /locus_tag="VF589_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX8257997.1"
                     /translation="MKKADVAEFYRRLADRTPNPKTELDYVNPYTLLVAVVLSAQSTD
                     AGVNLATRELFETVRTPEAMVELGEERLRQAIKTIGLFNTKAKNVIALSHRLIEQHGG
                     EVPADRDALVALPGVGRKTANVVMNTAFGAETFAVDTHIFRVANRTGLAPGKDPLAVE
                     LALDRVTPPPYRRDAHHLLILHGRYVCKARRPECWRCVAADLCRYQPKTPPPGEDPGK
                     AARGAGAKAPR"
     gene            complement(1324..2022)
                     /gene="dapB"
                     /locus_tag="VF589_10220"
     CDS             complement(1324..2022)
                     /gene="dapB"
                     /locus_tag="VF589_10220"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX8257998.1"
                     /translation="MTGIGLFGASGRMGKAIAEVLADRDDVSIVEDRCDVYIDFSAPD
                     GLDHHLDTAVSAGKPIVIGTTGLGEEHRRMIERAASEVAVLEAANMSLGVNLIAHLVR
                     ETAARLGEDWDVEIVEMHHRHKVDAPSGTALLLGRAAADGRGVALDSVSDRGRDGITG
                     DRGSGRIGFASLRGGSVAGDHQVIFAGEDERVEIGHRAESRAIFARGAVRAALWLVDR
                     PPGRYAMADVLGLR"
     gene            2394..4502
                     /locus_tag="VF589_10225"
     CDS             2394..4502
                     /locus_tag="VF589_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8257999.1"
                     /translation="MALISITDIVRVDQEGLGGGHNDSQVVVQALSDGGFAIAWREYE
                     ENGSGSYRVFTRKYDGSLAAVGDMVKLDGTDRDPLSGNGTGRPSIAALDNGAFAVEYN
                     DLGDVTRYSNGAPYGAYWMWVAEMTAGGTIGAQTTPFGRPDPDDDQQANTIWVSAIAG
                     DGDGGYHYAFVQSLTEVSGHRLVVNGSVLETGLHSNGTAIGLNGVGVTDFGDGVFVTY
                     QRNVDSGDADLWGRFVGGAADGAEFRINTQTAGYQTLNSANDGIAVLSNGSVVVTWRD
                     TSSADDATDPGYGVHARIMGANGVPIGDEFVLSATPGGDEGNAAVAAMSGGRFIAVWH
                     DDVGTVAQVFSSTGERLGDQVKINDNLVTSVTVLADDRVVFAWNLPGQTVTQVFRFPD
                     KFGTGGDDRMRGGAGDDVLHGLAGNDLLIGYAGADELRGGAGNDVYYVDHAGDKVVEL
                     PDQGRDRVRSTVDHTLADNVEDLRLAGAAVTGTGNALDNQIFGSSGDNFLYGLAGDDE
                     LRGQSGIDRLEGGADADTLYGGLSGDHLLGDGGGDRLIGEAGNDMLEGGADIDTLLGQ
                     DGADTLLGGDGADTLNGGIQRDLLTGGAGGDIFLFDDGHSRETRAAADQITDFTRADG
                     DRINLRAIDAKSATPDVDDKFSFIGTQAFSGNGAGGQLRYVNSQGNTFIEGDVDGNGV
                     ADFIIRLDGVHALQATDFVL"
     gene            4526..5233
                     /locus_tag="VF589_10230"
     CDS             4526..5233
                     /locus_tag="VF589_10230"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX8258000.1"
                     /translation="MREIRNIFVLTGAGISAESGVATFRGPDGLWEGHRVEDVATPEA
                     FERDPALVQKFYDARRAKLRTVEPNAAHRALARLDAEWPGELFIVTQNVDDLHERAGA
                     KRVLHMHGELRSAWCLACDGRSRWEEDLADGPSCPGCGGGGTLRPDIVWFGEMPYGMD
                     RIDTALMKADLFVSIGTSGAVYPAAGYVQTARYLGARTLELNLEPSQGSFFFDESRIG
                     RAGELVPAWVEEMLATS"
BASE COUNT          842 a         1786 c         1763 g          852 t
ORIGIN      
        1 tccattgatc accaacccca ccagcacgag gccgtcgatg aaggtcagcc cagtaatagt
       61 tgcggccgtc actacgctgg ctgccggcaa ggtcgtgaga gcgctcattc ggacagctat
      121 ggcctcttcc ttaggtccgg cttcgaaacg cttttcgtcg tccggtccgt ccagtcccag
      181 gtccgcgact cacatggcag ccaggaactt ccgactacgt ttactgggaa acctagcccg
      241 cggctctcgg tggaggcgtg acgccggtca cgctacatat cgcccgctaa ctcgcctggt
      301 ccctgcctat tttcggcagg aggggagggg gcggctaagt acttgaaaag gtggcggaca
      361 ccactcggcc aacctaggac gcaccgatcg agggtgaagg ttggcgcccg cttcggcccg
      421 gatgctcgtc ccaggatgtt caacgaaagc cgaggagttt ttccgtacct tttccgtacg
      481 agcccgacag gaattggcta agtcattgaa aagatggcgc acccgacagg attcgaacct
      541 gtggcctctg ccttcggagg gcagcgctct atccagctga gctacgggtg cctgggcgag
      601 ccgcttagca aagcccgggt gagggggcta gagcctttca gcgtggcgct ttggcgccgg
      661 cgccccgcgc agccttgcct ggatcctcgc cgggtggcgg cgtcttcggc tgatagcggc
      721 acaaatcggc ggccacgcag cgccagcatt cggggcggcg cgccttgcag acgtagcggc
      781 cgtgcaggat cagcagatgg tgggcgtcgc gccggtaggg cggcggcgtc acccggtcga
      841 gcgcgagttc gacggcgagc gggtccttgc ccggcgcgag gccggtgcgg ttcgcgaccc
      901 ggaagatgtg ggtgtcgacc gcgaacgtct cggccccgaa ggcggtgttc atcaccacgt
      961 tggcggtctt gcggccgacc ccgggcagcg cgaccagcgc gtcgcggtcg gccgggacct
     1021 cgccgccatg ctgttcgatc agcctatggc tcaaggcgat cacgttcttg gccttggtgt
     1081 tgaacaggcc gatcgtcttg atcgcctgcc gcagccgctc ctcgccgagc tcgaccatcg
     1141 cttccggcgt acgcaccgtt tcgaacagct cgcgcgtggc caggttcacg cccgcgtcgg
     1201 tcgactgggc cgacagcacc accgccacca gcaaggtata ggggttcaca tagtcgagct
     1261 cggtcttcgg gttgggcgtg cggtcggcga gccgccggta gaattccgcg acgtcggcct
     1321 ttttcacctc agccccagca cgtcggccat ggcgtagcgg ccgggcgggc ggtcgaccag
     1381 ccacagcgcc gcccgcaccg cgccgcgcgc gaagatcgcc cggctctcgg cgcggtggcc
     1441 gatctcgacc cgctcgtcct cgccggcgaa gatcacctgg tggtcgcccg ccaccgagcc
     1501 gcccctgagc gacgcgaagc cgatccggcc gctgccgcgg tcgccggtga tgccgtcgcg
     1561 gccgcggtcg ctcacgctgt cgagcgccac gcccctcccg tccgccgcgg cccggccgag
     1621 cagcagcgcc gtgcccgagg gagcgtcgac cttgtggcga tggtgcatct cgacgatctc
     1681 cacgtcccaa tcctcgccga gccgcgccgc cgtctcgcgc acgagatggg cgatgaggtt
     1741 gacgcccagc gacatgttgg ccgcctccag caccgccacc tcgctcgcgg cgcgctcgat
     1801 catccggcga tgctcctcgc cgagcccggt cgtgccgatc acgatcggct tgccggccga
     1861 aacggcggtg tcgagatgat ggtcgagccc gtcgggcgcc gagaagtcga tgtaaacgtc
     1921 gcagcgatcc tcgacgatgc tgacgtcgtc gcggtcggcc agcacctccg cgatcgcctt
     1981 gcccatccgc ccgctggcgc cgaacagccc tatgcctgtc atgaccttag ccttgccttt
     2041 gccgctccat gggcgaggtc cgcgacattc gcaacattgt gatccccgtg ctccccgcta
     2101 tttcgtcgag ggcgtcctct gccggaaatc aacatgatgc aggttgcggc tcccgtttcc
     2161 ggcctcgaca aggcgcggtc cgagtggcac cttccccgcc ggcgtcgcga gtctcggcgg
     2221 cgctttcgcg ttatggggta acgctggggg cgagcatggc cggggccatc tgccgggatc
     2281 aggatcgcgc ttcgaacgcc gcttgcatcg ggcaggccgc agcggcgcgc ccacatcatc
     2341 agcgaggcga tcgcgcgccc ggataagcgg gcaaggcaaa ggggggacca gccatggctt
     2401 tgatcagcat caccgacatc gtccgtgtcg accaggaagg cctcggcggc ggtcataatg
     2461 acagtcaggt cgtcgtccag gcgctgagcg acggaggctt cgcaatcgcc tggcgcgaat
     2521 atgaggagaa tggatccggc tcctaccgcg tgttcacgcg caaatacgat gggtcgctcg
     2581 ccgcggtggg cgacatggtc aagctcgacg gcaccgaccg cgacccgctc tccggcaacg
     2641 gcaccggccg gccgtcgatc gcggcgctgg ataacggcgc cttcgcggtc gagtataacg
     2701 atctcggcga tgtaacccga tattcgaatg gcgcgcctta tggggcttac tggatgtggg
     2761 tggccgaaat gaccgccggc gggacgatcg gcgcccagac aaccccgttc ggccggcccg
     2821 atccggacga cgatcagcag gccaacacga tctgggtcag cgcgatcgcg ggcgatggcg
     2881 acggcggcta tcattatgcg tttgttcaat cgctgacgga agtgtccggg cacaggttgg
     2941 tggtcaacgg atcggtgctg gagactggcc tacattccaa tggcaccgcg atcggcctga
     3001 acggcgtcgg ggtcaccgac ttcggagatg gtgttttcgt aacatatcag cgtaacgtag
     3061 actcaggcga tgccgatctc tggggccgtt ttgtcggcgg cgccgccgat ggcgctgagt
     3121 tccggatcaa cacccaaacc gccggctacc agacgctgaa cagcgccaat gacggcatag
     3181 ccgttctgtc caacggatcg gtggtggtga cctggcggga cacttcctcc gccgacgacg
     3241 ccaccgatcc gggctatggc gtccacgcgc gcatcatggg cgcgaacggt gtgccgatcg
     3301 gcgacgaatt cgtgctgtcg gccacgcccg gcggcgacga aggcaacgcc gccgtcgccg
     3361 ccatgagcgg cgggcgcttc atcgcggtct ggcacgacga cgtcggaacc gtggcgcagg
     3421 tcttctcgtc caccggcgaa cggctcggcg accaggtcaa gatcaacgac aatcttgtca
     3481 cctccgtcac cgtgctggcc gacgatcgcg tggtgttcgc ctggaacctg cccggccaga
     3541 ccgtcaccca ggtgttccgc ttccccgaca agttcggcac cggcggcgac gaccggatgc
     3601 ggggcggcgc gggtgacgac gtgctccacg ggctcgccgg caacgacctg ttgatcggct
     3661 atgcgggcgc cgacgagctg cgcggcggcg ccggcaacga cgtctattat gtcgatcatg
     3721 ccggcgacaa ggtcgtcgag ctgcccgacc agggccgcga ccgggtgcgc tccacggtgg
     3781 atcacacgct cgccgacaat gtcgaggatc tgcggctcgc cggggccgcc gtcactggca
     3841 ccggcaacgc gctcgacaat cagattttcg gcagttcggg cgacaatttc ctctacgggc
     3901 tcgccggcga cgacgagctg cgcggccaga gcggcatcga ccggctcgaa ggcggcgcgg
     3961 acgccgacac cctctatggc gggctcagcg gcgaccatct gctcggcgac gggggcggcg
     4021 acaggctgat cggcgaggcc ggcaacgata tgctggaggg cggcgccgac atcgacaccc
     4081 tgctggggca ggacggggcc gacaccctgc tcggtggcga cggggccgac accctcaacg
     4141 gcggcatcca gcgcgacctg ctgaccggcg gcgcgggcgg cgacatcttc ctgttcgacg
     4201 acggccacag ccgcgagacc cgcgcggcgg ccgaccagat taccgatttc acccgggccg
     4261 acggcgaccg catcaacctg cgcgcgatcg acgccaagtc ggcgacgccg gacgtggacg
     4321 acaagttcag cttcatcggc acccaggcct tttcaggcaa cggcgccggc gggcagcttc
     4381 gctacgtcaa ctcccagggc aacaccttca tagagggtga cgtggatgga aacggtgttg
     4441 ccgacttcat catccgcctc gacggcgtcc acgcgcttca ggcgacggat ttcgtcctgt
     4501 agccgcggcc ccctataacc gcccgatgag agagatacgg aacatcttcg tcctcaccgg
     4561 ggcgggcatc tcggccgaaa gcggggttgc gacctttcgc gggcccgacg gactgtggga
     4621 gggccaccgc gtcgaggacg tggccacgcc cgaagccttc gagcgcgatc cggcgctggt
     4681 ccagaaattc tacgacgccc gccgcgccaa gctccggacg gtcgaaccga acgccgccca
     4741 ccgggcgctc gcccggctcg acgccgaatg gcccggcgag ctctttatcg tcacccagaa
     4801 tgtcgacgac cttcacgaac gggcgggcgc gaagcgcgtg ctccacatgc atggcgagct
     4861 ccgctccgcc tggtgcctcg cctgcgacgg ccgctcccgc tgggaggagg atctggccga
     4921 tggcccgtcc tgccccggct gcggcggggg cggcacgctg cggcccgaca tcgtctggtt
     4981 cggcgagatg ccttacggga tggaccggat cgacacggct ctgatgaagg ccgatctgtt
     5041 cgtgtcgatc ggcacttcgg gcgcggtcta ccccgccgcc ggctacgtcc agaccgcccg
     5101 ctatctcggc gcccgcacgc tcgagctcaa cctcgagccg agccagggca gcttcttctt
     5161 cgatgaaagc cggatcggcc gggcagggga actggtgccc gcctgggtgg aggagatgct
     5221 ggcgacgagc tgaaacgtcg cag
//
LOCUS       DASVRK010000258         9933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_363028, whole genome shotgun sequence.
ACCESSION   DASVRK010000258 DASVRK010000000
VERSION     DASVRK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363028"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VF589_10235"
     CDS             <1..664
                     /locus_tag="VF589_10235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8258001.1"
                     /translation="SEVYRRWGWVGRVWTWHEAADTRLFHPPTDERERDGLVWIGNWG
                     EGERTKELGDFLFRPARDAALPLDIFGVRYPPEALELLDRYGVRYRGWLPNARAPEIF
                     ARHLATVHVPRRYYSTILPGIPTIRVFEALACGIPLVSAPWADSEGLFRVGEDFLMVA
                     SRAEMTKALKRLRADPALRGALVASGLETIRARHTCGHRAEELLAIAEALAAPAPLRS
                     VA"
     gene            661..1767
                     /locus_tag="VF589_10240"
     CDS             661..1767
                     /locus_tag="VF589_10240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8258002.1"
                     /translation="MKIAFYGSSLLSSYWNGAATYYRGLLRDLAGRGYDVTFYEPDAF
                     DRQQHRDIDPPEWARSVVYPATEAGLRSVLAQAGGADIVVKANGVGVFDAELLEGIVA
                     HSRPGALRIFWDVDAAATLEEMRADDSHVVRRMLPELDMVLTYGGGPPVVEAYEGFGA
                     AQCVPVYNALDPTTHHPVAPDEAFACDLAFLGNRLPDREARVEEFFLKAAGLLAERSF
                     LIGGNGWETKAMPANVRHIGHVYTHQHNAFNCTPLAVLNVARDSMAHIGFSPATRVFE
                     AAGAAACLITDAWEGIELFLKPDEEVLVARDGQDVAELVAALTPERARAIGQAALRRT
                     LAENSYAHRGAQVDALLRAALPNRHPREMAEAQA"
     gene            1764..2867
                     /locus_tag="VF589_10245"
     CDS             1764..2867
                     /locus_tag="VF589_10245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8258003.1"
                     /translation="MSEQPLRLVVLGLSLSSSWGNGHATTFRALLKAFAARGHDILFL
                     EREVPWYASNRDLQDPDYCQLEYYADLAGLERWRSDIAHADAVIVGSYVPEGVAVGRF
                     VQATALGVTAFYDIDTPVTLAKLARGDFEYLSPEIIPGYDLYLSFTGGPTLRFIEERY
                     KSPAARALYCSVDPDAYPMLDVPKTWDLSYLGTYSPDRQPTLERLLIEPARRLPDRRF
                     VVAGPQYPGDIDWPANVERIEHLPPRDHARFYAASRFTLNVTRADMIAAGWSPSVRLF
                     EAAACAVPVISDAWQGIDSLFAPGREILLAGSPDEATDILTGRSEAEAAAVGQAARAR
                     VLAGHTAAHRAAELETSLREAAGAAVPELVEAS"
     gene            2980..4026
                     /locus_tag="VF589_10250"
     CDS             2980..4026
                     /locus_tag="VF589_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX8258004.1"
                     /translation="MKNGTSKKALVTGGAGFIGSHLIDALLAQGASVTCLDNFQTGRP
                     QNLGHLESEGRFDLVEADVVDDLPREIAGRDFTHIYNLACAASPPHYQADPEHTMLTC
                     VLGTRNLLRLAETTGARFLLTSTSEVYGDPEVHPQTEDYRGWVNCTGPRACYDEGKRA
                     AETLAFDFARMGRADVRVARIFNTYGPRMRPDDGRVVSNVICQALAGDDITIYGDGSQ
                     TRSFCYVSDLVDGLMRLMDSDSAIDGPVNLGNPNELTVGDLVERVLALTGSRSRLTYL
                     PLPVDDPRRRKPDISRALELLGWQPRVALPQGLETTAAWFADAANDLPGRWRMAAQPA
                     GVGDRGLIMGVAAE"
     gene            complement(4218..4589)
                     /locus_tag="VF589_10255"
     CDS             complement(4218..4589)
                     /locus_tag="VF589_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8258005.1"
                     /translation="MAEAPRKSDQAFRTIGELSADLGIPQHILRYWETRFPQLRPLQR
                     AGNRRYYRPADVALANRIHRLLNRDGYTIRGVQQLLASGVADGEEVIPPVAPAKADGK
                     SFPAEELRSLRKKLRTALDEG"
     gene            complement(4594..4917)
                     /locus_tag="VF589_10260"
     CDS             complement(4594..4917)
                     /locus_tag="VF589_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX8258006.1"
                     /translation="MADAGTIRKPEVGTLTRADLADVVHREIGLSRAESANIVERILH
                     HMCHALSEGENVKISGFGSFILRDKGERIGRNPKTGVEVPIAPRRVLTFRASQIMRER
                     IVDGG"
     gene            complement(4999..5961)
                     /locus_tag="VF589_10265"
     CDS             complement(4999..5961)
                     /locus_tag="VF589_10265"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX8258007.1"
                     /translation="MSRRSVIAGTGSALPPRRVTNAELAERIDTTHEWIVERTGIHAR
                     HIAADGETTATLATEAARKALDDAGVRADQVGLIVLATATPDQTFPASATRVQTALGI
                     DDCIAFDVAAVCTGFLYALSVADNMLKGGMADYALVIGSETFSRILDWEDRTTCVLFG
                     DGAGAILLHSVDAQDKGVLATKLHADGRHNDLLFVDGGASTTGTIGKLRMKGKEVFRH
                     AVVNLAAVLNETLEAAGHTADEVDWVVPHQANKRILDATAKKLGLDPARVIVTVDQHA
                     NTSAASVPLALDVAVRDGRIQRGDLIVLEAMGGGFTWGAAVVRY"
     gene            complement(5958..7004)
                     /gene="plsX"
                     /locus_tag="VF589_10270"
     CDS             complement(5958..7004)
                     /gene="plsX"
                     /locus_tag="VF589_10270"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX8258008.1"
                     /translation="MGASPRIAIDAMGGDTGPAVMVAGAACAHERRDDLCFLLFGDEA
                     AIRAELLKHPRLEGSTEVVHTKDVIASEEKPTQAIRRAKTSSLGRAINAVKTGQANAA
                     LSAGNTGAMMAMAKLALRTMPGIHRPALAALLPTLGENDLVMLDLGANTECDTQNLVQ
                     FAIMGAAYSRVVLDLERPRVKLLNIGTEELKGTDELKDAAAILRDADYLGMSFDGFTE
                     GDKISVGDVDVVVTDGFSGNIALKTAEGTARFVVDLLRRAFTSSVRSKIGFLISRPAT
                     ELLRHHLDPNNHNGAVFLGLNGLVVKSHGSANEKGVANAIKVAARMVREDLTRKIAED
                     LENVSSHTQLAAAK"
     gene            complement(7032..7211)
                     /gene="rpmF"
                     /locus_tag="VF589_10275"
     CDS             complement(7032..7211)
                     /gene="rpmF"
                     /locus_tag="VF589_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX8258009.1"
                     /translation="MAVPKRKTSPSKRNMRRSHHALKPVNFQECANCGELHLPHNLCA
                     ACGHYNGREIRPVEA"
     gene            7404..7850
                     /locus_tag="VF589_10280"
     CDS             7404..7850
                     /locus_tag="VF589_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8258010.1"
                     /translation="METLPITLTIAGAAALLNVWLGFRVSLLRRRHKVALGDGGESAV
                     TARMRAHANFVEYTPFFLILLGLVEMARGSATWLWLVAMLFIVGRILHPFGMDRPGAN
                     ALRVVGISLTWLTLLGLAAYALTIPYLDRGRPPTMTYASASAASGS"
     gene            complement(7847..8509)
                     /locus_tag="VF589_10285"
     CDS             complement(7847..8509)
                     /locus_tag="VF589_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8258011.1"
                     /translation="MPTPPLRATIIPVTPLQQNCTLLWCTRTMRGAFVDPGGDLDRLK
                     AAAAAQNVTIEKIILTHGHIDHCGSAKPLAEELGVPIEGPHEADKFWLDRLEEDGARY
                     GILGVPFEPDRWLVEGDQVTVGDIAFDVYHCPGHTPGHVIFHQRASRIAIVGDVLFQG
                     SVGRTDFPRSDPQQLIDAIVTKLWPLGDDTAFLPGHGPPSNFGHERRTNPFVADRVLA
                     RA"
     gene            complement(8628..9008)
                     /locus_tag="VF589_10290"
     CDS             complement(8628..9008)
                     /locus_tag="VF589_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEX8258012.1"
                     /translation="MNTVKLGAAGLAIAVAALIIPATVQAAQHEPVVVEGSAEPRPTI
                     RVSFADLNLANKAGLARLNSRIAQAADRLCIEEADHTVATVMAGHACRRSAVAGAEEQ
                     ISRAVSTFGNPAYAALTQIMVTLR"
     gene            complement(9285..>9933)
                     /locus_tag="VF589_10295"
     CDS             complement(9285..>9933)
                     /locus_tag="VF589_10295"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEX8258013.1"
                     /translation="PALHRLLMERAERTLQDPLTVLDWVNLWALAVNEENAAGGRVVT
                     APTNGAAGIVPAVLRYYQRFTPRTSYDGVHDFLLTAAAIGSLFKENASISGAEVGCQG
                     EVGVACSMAAAGLTAALGGTPRQIENAAEIGMEHNLGLTCDPVGGLVQVPCIERNAVG
                     AVKALEASRLALVGDGTHRVSLDQVIETMRKTGLDMNVRYKETSLGGLAVNVVEC"
BASE COUNT         1519 a         3397 c         3374 g         1643 t
ORIGIN      
        1 gtccgaagtg tatcggcgtt ggggctgggt cggccgggtc tggacctggc acgaggcggc
       61 tgacacgcgg ctgtttcatc cgccgaccga tgaacgcgag cgcgacggct tggtctggat
      121 cggcaattgg ggcgagggcg agcgcaccaa ggagctgggg gatttcctgt tccgcccggc
      181 ccgcgacgcc gcgctgccgc tcgacatttt cggagtccgc tacccgcccg aggcgctgga
      241 gctgctcgac cgctacggcg tccgctatcg cggctggctc cccaacgccc gggcgcccga
      301 aatcttcgcc cgccacctcg ccaccgtcca cgtgccgcgc cgttattatt cgaccatcct
      361 gccgggcatc ccgaccatcc gggtgttcga agcgctcgcc tgcggcatcc cgctggtctc
      421 cgcgccctgg gcggacagcg aaggcctgtt ccgggtcggc gaggatttcc tgatggtcgc
      481 cagccgcgcg gagatgacga aggcgctgaa gcggcttcgg gccgatcccg ccctccgcgg
      541 cgcgctcgtc gccagcggcc tcgaaaccat ccgcgcccgg cacacttgcg gccaccgcgc
      601 cgaggagttg ctggcgatcg ccgaagcact cgcggcgccc gcgccgctga ggagcgtcgc
      661 atgaagatcg ccttttacgg atcgagcctg ctctcctctt attggaacgg cgcggcgact
      721 tattatcgcg ggctgctgcg cgacctcgcc gggcgcggct acgacgtcac tttctacgag
      781 cccgacgcgt tcgaccggca gcagcaccgc gacatcgatc cgccggaatg ggcgcgctcg
      841 gtcgtctacc ccgccaccga agcggggctg cgctcggtgc tggcccaggc gggaggcgcc
      901 gacatcgtcg tcaaggcgaa cggcgtgggc gtgttcgacg ccgaattgct cgaggggatc
      961 gtcgcccaca gccgcccggg cgcgctgcgg atcttctggg acgtcgatgc cgccgccacg
     1021 ctcgaggaga tgcgcgccga cgacagccac gtggtccgcc gcatgctgcc cgagctcgac
     1081 atggtgctga cctatggcgg cggtccgccg gtggtcgagg cctatgaagg gttcggcgcg
     1141 gcgcaatgcg tgccggtcta caacgcgctc gatccgacca cccaccatcc ggtggcgccg
     1201 gacgaggcct tcgcctgcga cctcgccttc ctcggcaacc ggctgccgga ccgcgaggcc
     1261 agggtggagg aattcttcct gaaggcggcc gggctgctcg ccgaacgcag cttcctgatc
     1321 ggcggcaatg gctgggagac caaggccatg cccgccaacg tccgccacat cggccacgtc
     1381 tacacccacc agcacaacgc ctttaactgc accccgctcg ccgtcctcaa cgtcgcccgc
     1441 gactcgatgg cgcatatcgg cttctcgccg gcgacccgcg tgttcgaggc ggccggcgcc
     1501 gccgcctgcc tgatcaccga cgcctgggaa gggatcgagc tgttcctgaa gccggacgag
     1561 gaagtgctcg tcgcccgtga cggccaggac gtcgccgagc tggtcgccgc cctcaccccc
     1621 gagcgcgccc gcgcgatcgg ccaggccgcg ctccgccgca ctttggccga aaacagctac
     1681 gcccaccgcg gcgcgcaggt cgacgcgctg ctgagggcgg cgctccccaa tcgtcatccc
     1741 cgcgagatgg cggaggcgca ggcatgagcg agcagccgct ccgtctcgtc gtgctcgggc
     1801 tctcgctctc ctcctcatgg gggaacggcc acgccaccac cttccgggcg ctcctgaagg
     1861 cgttcgccgc ccgcggccac gacatattgt tcctggagcg cgaggtcccc tggtacgcct
     1921 ccaaccgcga ccttcaggac cccgattact gccagctcga atattatgcc gacctcgccg
     1981 gcctcgagcg ctggcggagc gacatcgccc atgccgatgc ggtgatcgtc ggctcctacg
     2041 tgcccgaggg cgtcgccgtc ggccgcttcg tccaggcgac cgcgctcggc gtcaccgcct
     2101 tttacgacat cgacacgccg gtcacgctcg ccaagctcgc ccgcggcgat ttcgaatatc
     2161 tctcgcccga gatcatcccc ggctacgacc tttatctctc cttcaccggc ggcccgacgc
     2221 tccgcttcat cgaggaacgc tacaagtcgc ccgccgcccg cgcgctctat tgctcggtcg
     2281 atcccgacgc ctatccaatg ctcgacgtgc cgaagacatg ggacctctcc tatctcggca
     2341 cctacagccc ggaccgccag ccgacgctgg agcggctgct gatcgagccg gcccggcgcc
     2401 tccccgaccg ccgcttcgtc gtggccggcc cgcaatatcc gggcgacatc gactggcccg
     2461 ccaatgtcga gcgcatcgag cacctgccgc cgcgcgatca cgcgcgattc tacgccgcct
     2521 cgcgcttcac cctgaacgtc acccgcgccg acatgatcgc cgccggctgg tcgccctcgg
     2581 tgcggctgtt cgaggccgcc gcctgcgcgg tcccggtcat ctcggacgca tggcaaggca
     2641 tcgacagcct gttcgccccc ggccgcgaga tcctgctcgc cggaagcccg gacgaggcga
     2701 ccgacatcct caccggccgc agcgaagccg aggccgcggc ggtcggccag gcggcgcgcg
     2761 cgcgcgtcct ggcgggccac accgccgccc accgcgccgc cgagctcgaa acctctttgc
     2821 gcgaagcggc cggcgccgcc gtcccggaac tggtggaggc ctcgtgactc gcggaggaag
     2881 ctaggtcgtt gcttacatga accaattgtt acgagccgcg ttcagccggt taactaagtt
     2941 gaacaagaag tagaagaaga agctgcaagg gagccgcctg tgaagaacgg aacgagcaaa
     3001 aaggcgctgg tgacaggcgg cgccggattc atcggttcgc acctgatcga cgcgctgctg
     3061 gcgcagggcg cgagcgtgac ctgcctcgac aatttccaga ccggccggcc acaaaatctc
     3121 ggccatctcg aaagcgaagg gcgcttcgac ttggtcgagg ccgatgtggt cgacgacctg
     3181 ccgcgcgaga tcgccgggcg cgacttcacc cacatctaca atctcgcctg cgccgcctcg
     3241 ccgccccatt atcaggccga tcccgagcat acgatgctga cctgcgtgct cggcacccgc
     3301 aatttgctcc gcctcgccga gacgaccggc gcccgcttcc tgctgacctc gaccagcgag
     3361 gtctatggcg atcccgaagt ccacccgcag accgaggatt atcgcggctg ggtcaactgc
     3421 accggcccgc gcgcctgcta tgacgagggc aagcgcgccg ccgagacgct cgccttcgac
     3481 ttcgcccgca tgggccgcgc cgacgtccgg gtcgcccgca tcttcaatac ttacgggccg
     3541 cggatgcgcc ccgacgacgg ccgcgtcgtc tccaacgtca tctgccaagc gctggccggg
     3601 gacgacatca ccatctatgg cgacggctcc cagacgcgca gcttctgcta cgtcagcgac
     3661 ctggtcgacg ggctgatgcg gctgatggat tcggacagcg cgatcgacgg gccagtcaat
     3721 ctcggcaacc ccaatgagct caccgtcggc gatctggtcg agcgcgtcct cgccctcacc
     3781 ggctcacgct ccaggcttac ttatctcccg ctcccggtcg acgacccgcg ccggcgcaag
     3841 cccgacatca gccgtgcgct ggagctgctc ggctggcagc cgcgagtcgc gctgccgcag
     3901 ggcctcgaga cgaccgcggc ctggttcgcc gacgcggcca acgatcttcc gggccgctgg
     3961 cgcatggccg cccagccggc cggcgtcggc gaccgcgggc tgatcatggg cgtcgcggcg
     4021 gaatagcggg ggcggttcgc cggccgaatg acttgagccg aattggcggg gatcgcctgg
     4081 tggtctctgc caccggtcca gattcctcgc atgccggact ctcgtcattc cagcgcaagc
     4141 tggaatctca cttcgtcccg ccggcccgcg tcgtagggaa gggagattcc agctttcgct
     4201 ggatgacgat gtccgggtca gccctcgtcg agggcggtgc ggagcttctt gcgcagcgag
     4261 cgcagttcct cggcggggaa ggattttccg tcggctttgg ccggagccac aggcggaatc
     4321 acctcctcgc cgtcggcgac gccgcttgcg agcagctgct ggacgccgcg aatcgtatat
     4381 ccgtcgcggt tgagcaggcg gtgaatgcga ttggcgagcg ccacgtcggc ggggcggtaa
     4441 tagcggcggt tgccggcgcg ctggagcggc cggagctgag ggaaacgggt ttcccaatag
     4501 cgcaggatat gttgcggaat tccgaggtcg gccgaaagct cgccgatggt gcgaaaggcc
     4561 tggtcgctct tgcggggagc ctcggccaca gcctcagccg ccgtctacga tccgctcgcg
     4621 cataatctga ctggcgcgga aggtaagcac ccggcgagga gcgatcggaa cttcgacgcc
     4681 cgttttcgga ttgcggccga ttcgctcgcc cttgtcacgc aggatgaagc ttccgaaccc
     4741 ggaaatcttc acattctcac cctcggacag cgcgtggcac atgtgatgaa ggatgcgctc
     4801 gacgatgttc gccgactcgg cccgggacag cccgatctcc cgatgtacga catcggccaa
     4861 gtcggcccgc gtaagcgttc cgacttccgg cttcctgatt gtcccagcat cagccattgg
     4921 tattccctcc ccgagaataa tccggaaaga ttagtgtaac ttgagggtaa gcgcaatatc
     4981 gattgcaata aatcgtcatt aataacggac gaccgcggct ccccatgtga aaccgccgcc
     5041 catcgcctcc agcacgatca ggtcgccgcg ctggatgcgg ccgtcgcgca ccgcgacgtc
     5101 gagcgccagc ggcaccgagg cggccgaggt gttggcatgc tgatcgacgg tgacgatcac
     5161 ccgcgccgga tcgagtccga gcttcttggc cgtggcgtcg aggatgcgct tgttggcctg
     5221 gtgcgggacc acccaatcga cctcgtcggc agtgtggccg gcggcctcga gcgtttcgtt
     5281 gagcacggcg gcgaggttga ccaccgcatg gcggaagacc tccttgccct tcatcctcaa
     5341 tttgccgatc gtccccgtgg tcgaggcgcc gccgtcgacg aacaacaggt cgttgtggcg
     5401 gccgtcggcg tggagcttgg tcgcgagcac ccctttgtcc tgggcgtcga ccgaatggag
     5461 caggatcgcg ccggcgccgt cgccgaacag gacgcaagtg gtccggtcct cccagtcgag
     5521 gatccggctg aacgtctccg atccgatcac caaagcgtaa tcggccatgc cgcccttcag
     5581 catattgtcg gcgacggaca gggcgtagag gaagccggtg cagacggcgg cgacgtcgaa
     5641 agcgatgcag tcgtcgatgc cgagcgcggt ctggacgcgc gtggcgctgg ccgggaaggt
     5701 ctggtctggc gtggccgtgg cgagcacgat cagcccgacc tggtcggccc gcaccccggc
     5761 gtcgtcgagc gccttgcggg ccgcttccgt ggccagcgtc gcggtggtct cgccgtcggc
     5821 ggcgatgtgg cgggcgtgga tgccggtccg ctcgacgatc cattcgtgag tcgtgtcgat
     5881 ccgctcggca agctcggcat tggtcacgcg ccgtggcggc agcgccgatc ccgtgcccgc
     5941 gatcacggaa cggcggctca tttggcggcc gccagctggg tatgggagga gacgttctcg
     6001 agatcctcgg cgatcttcct cgtgaggtcc tcccgcacca tccgggcggc gaccttgatc
     6061 gcgttcgcca cccctttttc gttggcgctg ccgtggctct tcacgacgag gccgttcagc
     6121 ccgaggaaga cggcaccgtt atggttgttc ggatcgagat ggtggcgcag caattcggtc
     6181 gccgggcgcg agatcaggaa gccgatcttg gagcgcaccg aactggtgaa ggcgcggcgg
     6241 aggagatcga cgacgaagcg ggcggtgccc tcggcggtct tgagcgcgat gttgccggaa
     6301 aagccgtccg tcaccacgac gtcgacgtcg ccgacggaga tcttgtcgcc ttccgtgaag
     6361 ccgtcgaagc tcatgcccag ataatcggcg tcgcgcagga tcgccgcggc gtccttgagc
     6421 tcgtcggtgc ccttgagctc ctcggtgccg atgttgagca gcttcacgcg cggcctttcg
     6481 agatcgagca cgacccggga ataagcggcg cccatgatcg cgaactgaac cagattctgg
     6541 gtgtcgcact cggtgttggc gccgagatcg agcatcacca ggtcgttttc gcctagcgtc
     6601 ggcagcaaag cggcgagcgc cggccggtgg atgccgggca tggtcctgag cgcgagcttg
     6661 gccatcgcca tcatcgcgcc ggtattgccg gcggacagcg cggcattggc ctggccggtc
     6721 ttgacggcat tgatggcgcg gccgagcgag ctggtcttgg cgcggcggat cgcctgggtc
     6781 ggcttctcct cggaggcgat cacgtccttg gtgtggacga cctcggtcga gccttcgaga
     6841 cggggatgct tgaggagctc ggcgcggatc gcggcttcgt ccccaaacag caggaagcag
     6901 agatcgtcgc ggcgctcatg cgcgcaggcg gccccggcca ccatcacggc ggggccggta
     6961 tcgccaccca ttgcgtcgat tgcgatccgt ggcgacgcgc ccaccggtcc cgccttcccg
     7021 gatcgcagcc gttaagcctc taccggccgg atttcgcggc cattataatg gccgcaggca
     7081 gcgcacagat tgtgcggcag gtgcaattcg ccgcaattgg cgcattcctg gaagttcacc
     7141 ggcttcagcg cgtgatggct gcggcgcatg ttgcgcttgg acggcgaggt ttttctcttg
     7201 gggacggcca ttgtggcacc tgttccaata gtcttgagtg tgtgatgcgg gcgagcaccg
     7261 agccaggggt caatggccaa gcggccgcag gccctggcgg ccgagcgctc acgttgaagc
     7321 ggggccgcct atagcgaatt cccgcattgt tgcaagcacc ggctgcccgt gcaagctggg
     7381 gagcgacaca gggagatgca agcatggaaa cgctgccgat cacgctcacc atcgccgggg
     7441 ccgccgccct gctcaacgtc tggctgggct tcagggtcag cctgctcagg cggcgccaca
     7501 aggtggcgct gggcgacggc ggcgagagcg cggtgacggc acgaatgcgg gcgcacgcca
     7561 atttcgtcga atatacgccc ttcttcctga tcctgctcgg cctcgtcgaa atggcgcggg
     7621 ggtcggcgac ctggctgtgg ctggtggcga tgctgttcat agtcggccgc atcctgcatc
     7681 cgttcggcat ggaccggccc ggggccaacg ccctcagggt ggtaggcatc tcgctcacct
     7741 ggctgacttt gctcggcctt gccgcctacg ccctcaccat cccctatctc gaccggggcc
     7801 gccccccgac gatgacttac gcatccgcct cggcggcctc gggttcctag gcgcgggcca
     7861 gcacccggtc ggcgacgaac gggttggtgc ggcgctcatg cccgaaattg ctgggcgggc
     7921 cgtggccggg caggaaggcg gtgtcgtcgc cgagcggcca cagcttggtg acgatcgcgt
     7981 cgatcagctg ctgcgggtcg ctgcgcggga aatcggtgcg gccgaccgag ccctggaaca
     8041 gcacgtcgcc gacgatcgcg atccgcgatg cccgctggtg gaagatcacg tggccgggcg
     8101 tatggccggg gcagtgatag acgtcgaagg cgatgtcgcc gaccgtcacc tggtcgccct
     8161 cgaccagcca gcggtcgggc tcgaacggga cgcccaaaat gccgtagcgg gcgccgtcct
     8221 cctcgagccg gtcgagccag aatttgtcgg cctcatgcgg cccctcgatg ggcacgccga
     8281 gctcttcggc gagcggcttg gccgatccgc aatggtcgat atggccgtgg gtgagaatga
     8341 tcttctcgat cgtcacattc tgcgccgcgg ccgccgcctt cagccggtcg aggtcgccgc
     8401 cgggatcgac gaaggcgccg cgcatggtgc gcgtgcacca cagcaaggtg caattctgct
     8461 ggagcggcgt caccgggatg atcgtggcgc gaagcggcgg ggtcggcata agcggcccat
     8521 ggcgcgccgc ggcgggggat gcaagcatcc gagcggcaac ggattttaac gaaaaattgg
     8581 gcggcgccga ggaggacgcc gcccaaccca ccgcatcgct gggggcttta gcgaagggtg
     8641 accatgatct gcgtcagcgc cgcataagcg gggttgccga aggtcgagac cgcgcggctg
     8701 atctgctcct cggcgccggc gacggcgctg cggcggcagg cgtggccggc catgacggtg
     8761 gcgactgtat ggtcggcctc ctcgatgcag agccggtcgg cggcctgggc gatccggctg
     8821 ttgagccgcg cgaggcccgc tttgttggcg aggttgaggt cggcgaagct cacccggatg
     8881 gtcgggcgcg gctcggccga gccttcgacg accaccggct cgtgctgggc ggcctggacc
     8941 gtggccggga tgatgagcgc cgccacggcg atcgcaagac cggcggctcc aagtttcacg
     9001 gtgttcatgt cgcaatctcc tttctgcgtt gcgctcgcgg cgagctgttg aatgactgga
     9061 tggagggggt ctgagccgcg ccccgtccga cacgctttgg ataggcgagg agcgcgggcg
     9121 aatgcgcggc cgcttcgacc tgccgccggg cgcgttcgat cagcggacgg agcgtccgac
     9181 gaagcgccgc gaggcttcga tcagcggcgc gcggccgcgg tcacgggcgg atattcgggg
     9241 gagcaagcgc gcgggcgtgc ccgagtcgca tcaggccgag gcgctcagca ctccaccacg
     9301 ttgacggcga ggcctccgag cgaggtctcc ttgtagcgga cgttcatgtc gagcccggtc
     9361 ttgcgcatcg tctcgatcac ctggtcgagc gacacgcggt gggtgccgtc gccgaccaga
     9421 gcgaggcgcg aggcttcgag cgccttgacc gcgccgaccg cgttgcgctc gatgcacggc
     9481 acctggacga ggccgccgac cggatcgcag gtgaggccga gattatgctc catgccgatc
     9541 tcggcggcat tctcgatctg gcggggcgtg ccgccgagcg cggcggtgag cccggccgcc
     9601 gccatcgagc aggcgacgcc gacctcgccc tggcagccga cctccgcgcc ggaaatggag
     9661 gcattctcct tgaacagcga tccgatcgcg gccgcggtga gcaggaagtc gtggactcca
     9721 tcatagctcg tgcgcggcgt gaagcgctga tagtaacgca gcaccgccgg cacgattcca
     9781 gccgcgccgt tggtcggcgc ggtgacgaca cggccgcccg ccgcattctc ctcgttcacc
     9841 gccagcgccc agagattgac ccagtctagc acggtcagcg ggtcctggag cgtccgctcg
     9901 gcgcgctcca tcaggaggcg gtggagggcg ggt
//
LOCUS       DASVRK010000259        14311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_427728, whole genome shotgun sequence.
ACCESSION   DASVRK010000259 DASVRK010000000
VERSION     DASVRK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 14311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14311
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427728"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..2024)
                     /locus_tag="VF589_10300"
     CDS             complement(360..2024)
                     /locus_tag="VF589_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8258014.1"
                     /translation="MAGAAADGTADGAEPKASARKIIVASSLGTVFEWYDFYLYGLLA
                     GYISTQFFSGVNETTAFIFALAAFAAGFAVRPFGALVFGRIGDLVGRKYTFLVTMSLM
                     GVATFLVGLLPSYASIGVAAPVLLVTLRLLQGLALGGEYGGAATYVAEHAPAGKRGLY
                     TSFIQTTATLGLLAALLVVIGTRSLIGEEAFAAWGWRVPFLISILLLGVSLWIRMQLA
                     ESPVFQRMKKEGTTSKAPLRESFARWGNLKIVLIALVGAVAGQAVVWYTGQFYALFFL
                     ERMLKVDGATANILIAVALALATPGFVLFGWLSDKIGRKPIIMAGCALAVLTYFPLFE
                     ALTRAANPALHAAQQAAPVSVVADPESCAFQFDPIGRNKFDRSGCDIAKSFLAKAGVS
                     YANVAAPAGARAEVRIGAARIAAPEPQGGAAPAAAIAGFQAEAGAALKAVGYPEEADP
                     GRIERVRVVGILFLLVLYVTMVYGPIAALLVELFPSRIRYSSMSLPYHIGNGWFGGFL
                     PTTAFAMVAASGDIYYGLWYPIVVAGVTLVFGLLFLPETRGRDIEG"
     gene            2272..3516
                     /locus_tag="VF589_10305"
     CDS             2272..3516
                     /locus_tag="VF589_10305"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX8258015.1"
                     /translation="MNLLLAAALLLAPTPAGTLLVGNKGEDTLSFIDLASGRELARLP
                     TGRMPHEIAISPDGRQAAVVAYGGASLDIFDIAARRRLRSVALAPNQGPHGLVWLADG
                     RLVATTERSRSLTLVDPARGDAVSAVPTDEEGTHMVAVAPAGRLAFTANIAAGTISVI
                     DLVALRKLRDVPIGGAPEGIALTPDGKTLWVADLEGARVLAFDAAALAAPDGAALTPQ
                     IAIPLKLRGGSRAEVETALIAPLERELRALPEVASVQTTAEAGAGSVTATLRPGADLA
                     AATPLVAAALRRAGAGFPRGRAEAELRPYGAAPLAIVATGPRPIRVAISPDGRFVVTS
                     DFESGSLTLIDAATRAKIRTLPVSGTADAAQVTIIFSADGRRLYVAETGPDTVAELDF
                     PSGRLLRRLKAGRDGDGLAIAP"
     gene            3856..5583
                     /locus_tag="VF589_10310"
     CDS             3856..5583
                     /locus_tag="VF589_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258016.1"
                     /translation="MAVFAGSDAGETIRPDLVTDSVFRNPPGARPSDADDIINGGGGD
                     DIIEAAGGNDIVVGGDGDDQVLLGAGDDIFRAAWGAGNDIVNGSAGFDSYEWRGTDFG
                     NEFELRVSAAGMSLHRDAGGTLNDVERLRLDMGGGSDFVVLDNLGGSSLERVEIDFAA
                     GEGQEDFDAVNLLGDYGADRLVVRQAGPGLTIAGLAAAISIAGMSSGDEVQMFASSGD
                     DVINLSALLAGAPRMLIDGGTGDDLILPGMNADRISGGGGVDTVSYAHSPEAVRIGAV
                     GIAGDGGSAEGDMISLWSTLIGSAFDDTLAGNAAADTLIGGAGGDRLGGAGGDDILNG
                     GAGDDQLDGADGEDRMFGGAGDDRFEIDQAGDRVIEAAGGGTDRIYSSIDITLPANVE
                     NLSLDAFGGDLDGTGNALANGLGGNVDANTLLGGGGGDTLLGFAGNDILKGGVGIDTG
                     YGDMGDDRIYGGGGADIFGGGTGRDWFVFDTAPGPGQADTLTDFTRADDTIYLKRTIF
                     AGLPAGTLAADAFVLGTGAQDAEDRVIYDAATGRLFFDPDGTGGAAQLLFARVDPDTE
                     LGATDFRGF"
     gene            5788..7290
                     /locus_tag="VF589_10315"
     CDS             5788..7290
                     /locus_tag="VF589_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258017.1"
                     /translation="MKLLKVIGCLSLVLGLLRLVVDGPAVAAVDLKSADPLASCFRPA
                     QIPLGAPVTLSNLGSGAQAASLSYITDQPVAFEPVDPRLRIRLRAMAGAAALPRGTLV
                     RVASGEFTLAQGRARLRSGTGAGAMLLDAADPVRFGHAAKASYVVAIKPVQVAPPAPG
                     AAARLLPAGSSGTVGLRSPVALQALQPIEVRPWLLGQRENIFPVRASGQRGLSDLAID
                     VRHPGIRFDPAALAMAGCAWSDSAPPVPAAVASLEGASPGAGKAQLAMPSELLPAFSA
                     IVPQTVYVALASADGGYAGYGAFTAIDRGWAGVGATLLAALLLWGCLNARRGDEKSWS
                     AWASGLFLDANSQPSLSLFQIFFWTLITVWGLIYVFIVTGELMNLTGTMLVLLGIAGT
                     GTVAARFLSRGSTGAPAAPAVRAPATRTEVDFAALLSTNGTFDLLKLQLFVFTLLIGA
                     FVVWRIADSGVFPELDTETLLLLGVSQGLYIGGKAAAPATGDAAEPGRPA"
     gene            7504..9285
                     /locus_tag="VF589_10320"
     CDS             7504..9285
                     /locus_tag="VF589_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX8258018.1"
                     /translation="MTTDQILAFAVIGGMMGLFIWGRIRYDLVAIIALLAAVALGIVP
                     PDKAFTGFADDIVIIVASALVISAAIARSGIVEAAIQRLAGRFNRMGSQLIILVGSVT
                     ILSAMVKNIGALAMLMPSAFQMAKRSRTSPSCLLMPMAFGSLLGGLITLVGTSPNIIV
                     SRVREEMTGQPFGMFDYAPVGLGLSAAGLLFLRWGYKLLPTNRRAAPTMGEALDITDY
                     TTEVRIPPHSAADGVSIAEFHRLADGEVEVTGLHRGERRRAVPLPDARLTAGDVLILE
                     GESEALERAIAGLGLQLEGQHRGAATDTASANVGVIEAVIASRSMLVGDAAGRIALHD
                     RHGVNLLAVSRQGERLTERLRDLILQPGDVIVLQGPLSTLPARLRDLGCLPLAERPLR
                     LGSARRGLVPVAILGGAMALTAIGWVPVAIAFFAAAALIMLAGALPAREAYSHIEWPI
                     LVMIGALIPVSDSLRTTGATDLIAAWLSATAATLPAWAAVALILVAAMAVTPFLNNAA
                     TVLVMAPIAATFATELGYRPEAFLMAVAVGAGCDFLTPIGHQCNTLVMGPGGYRFGDY
                     ARLGAPLSLLVVLVGVPLILAVWPLRY"
     gene            complement(9295..10389)
                     /locus_tag="VF589_10325"
     CDS             complement(9295..10389)
                     /locus_tag="VF589_10325"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX8258019.1"
                     /translation="MAGGAAAAMPSLVRAAPAAPDMIIVNALGGLANPNPSKDWGAER
                     PGFEARVIADARGSGVTAINCTLGYVSGDMEPFEYSVRDIGLWDRRLRAHPGDLLKVL
                     TAADIPRARAEGKIGIIYGFQNAAMMGARPDRVEIFADLGVRVIQLTYNPANSIGDGA
                     MAPLNRGLTPLGRKLVERLNAQRIMVDLSHSGERTCLEAARASRQPISINHTGCRALT
                     DLPRNKSDAELRLVAERGGFVGIYFMPFLSPDSVATAEDVVAHIDHAVKVCGEDHVGI
                     GTDGGLTRVDDMAGYRRLIAGEIEERRKAGIGAKGENPDTLPFVEELQGPSQFRDLAE
                     RLRRRGYSERRVEKIMGLNFLAYAREVWGA"
     gene            complement(10453..11097)
                     /locus_tag="VF589_10330"
     CDS             complement(10453..11097)
                     /locus_tag="VF589_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8258020.1"
                     /translation="MIVYGSSLSPFVRKVLAFAAEKGIEVESKPVALWSDEPEFVESS
                     PFRKIPGFRDGDFAISDSSAIVAYLEAVKPEPNLIPAEAKARARAIWFDEFADTIVGA
                     CGTKMFFNRVVAPRFLKREGDLAVAEKAECEELPPILDYLERVIPDSGFLVEDRLTLA
                     DIAVASPFANFRHLGLDCGAGKRPKLMAYIEGMLARPSFAPWVARESAFLARAA"
     gene            11220..12401
                     /locus_tag="VF589_10335"
     CDS             11220..12401
                     /locus_tag="VF589_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX8258021.1"
                     /translation="MSSSAPSPSAEALIVRPVEDPADRKAFVDVAWTLNSGDPNWVPP
                     LKTEVHGLLDPRTNPWFEHAEARLFLAERGGRAVGRISAQVDSLVQAMPQHQGGGEGT
                     GHWGMFEAADAEVAAALIARAEDFLRGRRMRRAMGPISLSIWDEPGLLVKGHDHPPTV
                     MMGQNSPAYEAWIEAAGYRQARDLLTYELDITQTMPPLVQRIVASGERNPRIHVRKVD
                     KSRFDEEAALILGILNDAWSGNWGFVPLTDAEIAYAGRKLKPIVREDLIRVAEVDGDP
                     VAFMITLPDLNEFTRDLNGRLFPFGWAKLLWRLRNPKVKTMRVPLMGVVKRLQSTRLA
                     SQLAFMLIEYIRRSSIADYHATRGEIGWILDDNHAMRAIAETIESSINRVYRIYEKPL
                     S"
     gene            complement(12531..13145)
                     /locus_tag="VF589_10340"
     CDS             complement(12531..13145)
                     /locus_tag="VF589_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258022.1"
                     /translation="MIALLLAAGAAQLPSYEPGDAFVFNDGRVERVRGVSGERVTWSG
                     IGRTTYERSANPIVPILAWKVAGVGGRRDVGEAARRLWPLAPGKTARFPAVTIVDGKG
                     GARRSAAFWKCGVGRPAPVAVPAGTFEAWPIQCERYSASTMRLVERVQWDWAPELGHY
                     VRRRVTLYADGTTTEIRLAAALSGDGATTRRLRALAAKARAERR"
     gene            complement(13142..>14311)
                     /locus_tag="VF589_10345"
     CDS             complement(13142..>14311)
                     /locus_tag="VF589_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8258023.1"
                     /translation="DDRADAAEDAADDRADAADDLRDGRSGSGRDGRHDDNSGHGSGG
                     AEAGRGGGESRGVEYDREGYAVAAREVLALTDDPSDRDTLQALGFRVSSRESLPGTGL
                     ELLKIAVPETSTAEAALAQLVSRKSDSIFALNHLFAAGNVGKTPPGRRAARRPGGGGP
                     VGIIDGGVAPAALAGVKVEAVSFAPHASARQHHGTAVAALLADAGVGTIYSANIFDGR
                     NASAAAMVRALDWMAAKKVPVVNISLAGPPNPIVHRMIKILRLRGHAVVAAVGNDGPA
                     APPLYPAAYPEVIGVTAVDGRGSIYRRANRGPHTMLAAEGVDVTAVDSTGTPRSVSGT
                     SFAAPKVSARLARQLRAPDPAARERAVAGMVRAARDLGAPGRDPVYGYGLVDPES"
BASE COUNT         2119 a         5688 c         4430 g         2074 t
ORIGIN      
        1 ctgcgaagta tcgttcccct gcgaagcatc gttcccctgc gaaggcaggg gtccagtccc
       61 gggccggcgc ttcagcggcg gcccggtcat ccgccccgga tggaggcggg atatatgaaa
      121 cagctctgga agctcagcgc cgtcgagcag attaaccccg actggcgcga cctgtaccca
      181 accctcgtct gacaggcttc gcctggtctg gacccctgcc ttcgcagggg cacgagcgcc
      241 gcacctgttc ccctgcgaag gcaattgtgc ccctgcgaag gcaggggccc agccccgggc
      301 tgccgcttca gcggcagtcc ggtcatccgc cccgaaatca ttcgaagcgg cccgctactt
      361 tacccctcga tatcgcgccc gcgcgtctcc ggcaggaaca acaggccgaa caccaaagtc
      421 acccccgcca ccacgatcgg gtaccacagc ccgtaataga tatcgcccga ggccgcgacc
      481 atcgcgaacg ccgtcgtcgg caggaagccg ccgaaccagc cattgccgat atgataaggc
      541 agcgacatcg agctgtagcg gatccggctc gggaacaatt ccaccagcaa cgccgcgatc
      601 ggcccgtaga ccatggtcac atagaggacg agcaggaaca atatccccac cacccgcacc
      661 cgctcgatcc gccccggatc cgcctcttcc ggatagccga ccgccttcag cgccgccccc
      721 gcctccgcct ggaagccggc gatcgccgcc gccggcgccg ccccgccttg gggctcgggc
      781 gccgcgatcc gcgcggctcc gatccgcacc tcggcccgcg cccccgccgg cgccgcgaca
      841 ttggcgtagc tcacccccgc tttggccagg aacgacttgg cgatgtcgca gccgctgcgg
      901 tcgaatttgt tgcgcccgat cgggtcgaac tggaacgcgc aagactcggg gtccgcgacc
      961 acgctcaccg gcgccgcctg ctgcgccgcg tgcagcgccg ggttggccgc ccgcgtcagc
     1021 gcctcgaaca acgggaaata ggtcagcacc gccagcgcgc agccggccat gatgatcggc
     1081 ttcctcccaa tcttgtcgga cagccatccg aacagcacga agcccggcgt cgccagcgcc
     1141 agcgccaccg caatcagaat gttggccgtc gccccatcga ccttcagcat ccgctcgagg
     1201 aagaacaagg cgtagaattg cccggtatac cagaccaccg cctgccccgc gaccgcgccc
     1261 accaaagcga tcagcacgat cttcaaattg ccccagcgcg cgaagctttc gcgcaacggc
     1321 gccttggacg tcgtcccctc cttcttcatc cgctggaaca ccgggctttc cgcaagctgc
     1381 atccggatcc acagcgatac gcccagcagc aggatcgaga tcaggaacgg cacccgccag
     1441 ccccaggccg cgaacgcctc ctcgccgatc aggctgcgcg tcccgatcac caccagcaag
     1501 gccgccaaca gccccaaggt cgccgtcgtc tggatgaagc tcgtataaag cccgcgcttg
     1561 cccgccggcg catgctcggc gacataggtc gccgcgccgc catattcgcc gcccagcgcc
     1621 agcccctgca gcagcctgag cgtcaccagc agcaccggcg ccgcgacccc gatgctggca
     1681 tagctcggca gcagccccac gaggaaggtc gccaccccca tcaggctcat cgtcaccagg
     1741 aacgtatatt ttcgcccaac caggtccccg atccgcccga acaccaacgc cccgaacggc
     1801 cttaccgcaa accccgccgc aaacgccgcc agcgcgaaga tgaaggcggt cgtctcgttg
     1861 acccccgaga agaactgcgt cgaaatatat cccgcgagca gcccgtaaag gtagaaatca
     1921 taccattcga acaccgtccc caacgacgac gccacaatga tcttccgcgc actcgctttg
     1981 ggctccgccc cgtccgccgt gccgtccgcc gccgccccag ccatccgcac tcccctttcg
     2041 agccccgcca taacgggcca cccgccggga gcgcaatcga ccgctgctcc ggaagccgag
     2101 ccacgcgccc caccctgtcg acccaaccca cgcgggaacc aagcccaaaa aggaaaaccg
     2161 tcatcccagc gaaagctggg atctcccttc ccccttctgc cccaatcccc aacattgccc
     2221 cccaaccccc acccccctaa aaccgccgcc agcccccacg gagccgcccc gatgaacctc
     2281 ctcctcgccg ccgccctcct cctcgccccg acccccgccg gcaccctcct cgtcggcaac
     2341 aaaggcgaag acaccttaag cttcatcgac ctcgcctccg gccgcgagct ggcgcggctg
     2401 ccgaccggcc gcatgcccca cgaaatcgca atctcccccg acgggcgcca ggcggcggtg
     2461 gtcgcttatg gcggcgccag cctcgacatt ttcgacatcg ccgcccgccg ccgcctgcgc
     2521 agcgtcgcgc tcgcccccaa tcagggcccg cacggcctgg tctggctcgc cgacggccgc
     2581 ctcgtcgcca ccaccgagcg cagccgcagc ctcaccctcg tcgatcccgc ccgcggcgac
     2641 gccgtcagcg ccgtgcccac cgacgaggag ggcacccaca tggtcgccgt cgcccccgcc
     2701 ggccgcctcg ctttcactgc caacatcgcc gccggtacaa tcagcgtcat cgacctcgtg
     2761 gccttgcgca agctgcgcga cgtccccatc ggcggcgccc ccgaaggcat cgcgctcacc
     2821 cccgacggga agaccctgtg ggtcgccgac ctcgaaggcg cccgcgtcct ggcgttcgac
     2881 gccgccgcct tggccgcccc cgacggcgcc gccctgaccc cccagatcgc catcccgctc
     2941 aagctccgcg gcggctcccg cgccgaggtc gagaccgcgc tcatcgcccc gctcgagcgc
     3001 gagcttcgcg cgctccccga agtcgcttcc gtccagacca ccgccgaggc cggcgccggc
     3061 tccgtcaccg ccaccctgcg cccaggcgcc gacctcgccg ccgccacgcc gctcgtcgcc
     3121 gccgccctcc gccgcgccgg cgccggcttc cccaggggcc gcgccgaggc cgagcttcgc
     3181 ccttatggcg ccgccccgct cgccatcgtc gccaccgggc cgcgcccgat ccgggtcgca
     3241 atcagccccg acggccgctt cgtcgtcact tccgatttcg agagcggcag cctcaccctg
     3301 atcgacgccg ccacccgcgc caaaatccgc accctgcccg tcagcggcac cgccgacgcc
     3361 gcccaggtca cgatcatctt ctccgccgac ggccgccgcc tctacgtcgc cgagaccggc
     3421 cccgacaccg tcgccgaact cgacttcccc tccggccgcc tcctccgccg cctcaaggca
     3481 ggccgcgacg gcgacggcct cgccatcgcg ccgtgagccc cgtcattccc gcgaaagcgg
     3541 gagccagaga cgaccgcagg tcaatcccgc tcccctttcc gtcaccctga acttgtttca
     3601 gcgtccacgg ccgcgccagc ctccaatcca cccgaagcaa gcccgccacc cccgcagggc
     3661 acgcgcccca tccccgcccc agcgcgcgac caaagccccg ctcggaacga ccaacccgac
     3721 actttccccg cccggccgat tcgacgcccc ccgccgcgca gcctaagctg agccgccctc
     3781 gcctttctta cgagggcaag cttgcggacg cccgggcttc acggccccgg cgccggcgac
     3841 cgagggggac gcatcatggc tgtattcgct ggatccgacg cgggcgaaac catccgtccc
     3901 gacctcgtca ccgactcggt gttccgcaat ccgcccggcg ccaggccctc cgacgccgac
     3961 gacatcatca acggcggcgg cggcgacgac atcatcgagg ccgccggtgg caacgacatc
     4021 gtcgtcggcg gcgacggcga cgaccaggtc ctgctcggcg ccggcgacga catcttccgg
     4081 gccgcgtggg gcgccggcaa cgacatcgtc aacggcagcg ccggcttcga cagctacgaa
     4141 tggcgcggca ccgatttcgg caacgagttc gagctccgcg tctcggccgc cggcatgagc
     4201 ctccaccgcg acgccggcgg caccttgaac gacgtcgagc ggctgcgcct cgacatgggc
     4261 ggaggcagcg atttcgtcgt tctcgacaat ctcggcggct cgagcctcga gcgggtcgag
     4321 atcgacttcg ccgcgggcga ggggcaggag gatttcgacg cggtcaattt gctcggcgat
     4381 tacggcgccg accggctggt cgtccgccag gccggcccgg gtctcaccat cgcaggcctc
     4441 gccgccgcaa tctcgatcgc cggcatgagt tcgggcgacg aggtgcagat gttcgccagc
     4501 tctggcgacg acgtgatcaa cctgtccgcg ctcctcgccg gcgcaccgag aatgctgatc
     4561 gacggcggca ctggcgacga tctgatcctg cccggcatga acgccgaccg gatctcgggc
     4621 ggcggcggcg tcgacacggt cagctacgcg cactcgcccg aagcggtgcg gatcggcgcg
     4681 gttggcatcg cgggcgacgg cggctcggcc gagggcgaca tgatctccct gtggagcacg
     4741 ctgatcggct ccgccttcga cgacaccctc gccggcaacg ccgccgccga cactttgatc
     4801 ggcggcgccg gcggcgaccg gctcggcggg gccggcggcg acgacattct gaacggcggc
     4861 gccggcgacg accagctcga cggcgccgac ggcgaggacc gcatgttcgg cggcgccggc
     4921 gacgaccgct tcgagatcga ccaggccggc gaccgcgtca tcgaggcggc cggcggcggc
     4981 acggaccgca tctacagctc gatcgacatc actctccccg ccaacgtcga aaatctcagc
     5041 ctcgatgcgt tcggcggcga cctcgacggc accggcaacg cgctggccaa cggcctcggc
     5101 ggcaatgtcg acgccaacac gctgctcggc ggcggcggcg gcgacaccct gctcggcttc
     5161 gccggcaacg acatattgaa gggcggcgtc ggcatcgaca ccggctatgg cgacatgggg
     5221 gacgaccgca tctacggcgg cggcggcgcc gacattttcg gcggcggcac gggccgcgac
     5281 tggttcgtgt tcgacaccgc tcccggcccc ggccaggccg acaccctcac cgatttcacc
     5341 cgcgccgacg acaccatcta cctgaagcgc acgatcttcg ccggcctccc cgccggcacc
     5401 ctcgccgccg acgccttcgt gctcggcacg ggcgcgcagg atgccgaaga ccgcgtcatc
     5461 tacgacgccg ccaccggccg cctgttcttc gaccccgacg gcacgggcgg cgcggcccaa
     5521 ctcctgttcg cccgcgtcga tccagacacc gagctgggcg ccacggactt ccgcggcttc
     5581 tgaggggggc gcccgcccgc gccgccgcca ctgcccgtca tccccgcgca agcgggagcc
     5641 agagacgacc gcaggtcaat cccgctcccc ccgccgaaac ccgcactaat ctcgacaaaa
     5701 tacagtccgc cctcttatgc tggcgactcg gctccgttcg tttgcattgt tgcgtaggca
     5761 ggcttcgctc gatcagggga atgcaggatg aagctgctca aagtcatcgg ctgcttgtcg
     5821 ctcgtcctcg gtcttctgcg gctggtcgtt gacggccccg cggtcgctgc cgtcgacttg
     5881 aaaagcgccg atccgctcgc ttcatgcttc aggcccgcgc aaatcccgct cggcgccccc
     5941 gtgacgctgt cgaacctggg cagcggcgcg caggcggcat cgctctccta tatcaccgat
     6001 cagccggtcg ctttcgagcc ggtcgatccg cgcctgcgga tccgcctcag ggcgatggcc
     6061 ggggccgcgg cgctgccgcg cggaaccctg gtccgcgtcg cgtcgggcga gttcacgctg
     6121 gcgcaggggc gcgcgcggct ccgctcgggc acgggcgcgg gcgcgatgct cctcgacgcc
     6181 gccgatccgg tccggttcgg gcacgccgcc aaggcttcct acgtggtcgc catcaagccg
     6241 gtccaggttg cgccgcccgc gccgggggcg gcggcgcggc tgctgcccgc ggggagctcc
     6301 ggcacggtcg gcctgcgatc gccggtcgcg ctccaggcat tgcagccgat cgaggtgcgg
     6361 ccgtggctgc tcggccagcg cgagaatatc ttcccggtcc gcgcctccgg tcagcgcggg
     6421 ctcagcgacc tcgccatcga cgtccggcat ccgggcatca ggttcgatcc ggccgcgctc
     6481 gccatggccg gctgcgcctg gtccgacagc gcgccgccag tgcctgccgc ggtcgccagc
     6541 ctcgaaggcg cgagccccgg cgccggcaag gcccagctgg cgatgccgtc cgagctgctg
     6601 ccggccttct ccgccatcgt cccgcagacc gtctatgtcg ctctggcctc ggcggacgga
     6661 ggctatgccg gctacggcgc cttcaccgcc atcgatcgcg gctgggcggg tgtcggcgcc
     6721 accctcctcg cggcgcttct gctctggggc tgccttaacg cgcgccgcgg cgacgagaaa
     6781 tcgtggagcg cctgggcgtc cggcctcttc ctggacgcga attcgcagcc cagcctgtcg
     6841 ctgttccaga tcttcttctg gaccttgatc acggtctggg ggctgatcta cgtcttcatc
     6901 gtcaccggcg agctgatgaa cctcaccggc acgatgctgg tgctgctcgg cattgccggg
     6961 accggcaccg tcgccgcccg cttcctcagc cgcggctcga ccggggcgcc cgccgctccg
     7021 gccgtccgcg ccccggccac gcgcaccgag gtcgatttcg cggccttgct cagcaccaac
     7081 gggacgttcg atctgttgaa gctccagctg ttcgtcttca ccctgctgat cggcgcgttc
     7141 gtggtgtggc ggatcgccga ttccggcgtg tttccagagc tcgacaccga aaccctgctg
     7201 ctgctcggcg tcagccaggg cctctacata ggcggcaagg ccgccgcccc cgcgacgggc
     7261 gacgccgcgg agccgggccg gccggcataa gcccggatcg aggccggccc gtgcgcccgc
     7321 cgcgtcaccc ccccggaccc aatcccctcc gcccgcgttt gcaatcggcc cgcgtggagt
     7381 gggggcgcat cctcaggggc cttgcttgac gaccgaacac cgaccagacc tcgccaccgg
     7441 cgccgccggc cgcggcggcc ttcccccctt tccccctcga aaggaccact ttcgagggct
     7501 cggatgacca ccgaccagat cctcgccttc gccgtaatcg gcggcatgat gggcctcttc
     7561 atctggggcc gcatccgcta cgatctcgtc gccatcatcg cgctgctcgc cgcggtcgcg
     7621 ctcggcatcg tgccgcccga caaggcgttc accggtttcg ccgacgacat cgttatcatc
     7681 gtcgcctccg ccctcgtcat ctcggccgca atcgctcgct cgggcatcgt cgaggccgcc
     7741 atccagcggc ttgccggccg cttcaaccgg atgggctcgc agctcatcat cctggtcggc
     7801 tcggtcacca tcctctccgc catggtcaag aatatcggcg cgctcgccat gctgatgccg
     7861 tccgccttcc agatggccaa gcgctcgcgc acctcgccct cctgcctgct gatgccgatg
     7921 gcgttcggct ccttgctcgg cggcctcatc accctcgtcg gcacctcgcc caacatcatc
     7981 gtcagccggg tgcgcgagga gatgaccggc cagccgttcg gcatgttcga ttacgcgccc
     8041 gtcggcctcg gcctctccgc cgccggcctg ctgttcctgc gctggggcta taagttgctg
     8101 cccaccaacc gccgcgccgc gccgaccatg ggcgaggcgc tcgacatcac cgattacacc
     8161 acggaggtcc ggatcccgcc ccattcggcc gccgacggcg tcagcatcgc cgaattccac
     8221 cggctcgccg acggcgaggt cgaggtgacc gggcttcacc gcggcgagcg ccgccgcgcg
     8281 gtgccgctgc ccgacgcccg cctcaccgcc ggcgacgtgc tcatcctcga aggcgaatcg
     8341 gaagccttgg agcgcgccat cgccgggctc ggcctgcagc tcgaaggcca gcatcgcggc
     8401 gccgccaccg acaccgcttc cgccaacgtc ggcgtgatcg aggcggtgat cgccagccga
     8461 tcgatgctgg tcggcgacgc cgccggccgg atcgccctcc acgaccgcca cggcgtcaat
     8521 ttgctcgccg tctcgcgcca gggcgagcgg ctgaccgagc ggctgcgcga cctcatcctc
     8581 cagcccggcg acgtgatcgt gctgcagggg ccgctctcga ccctcccagc gcgcctccgc
     8641 gatctcggct gcctgccgct cgccgagcgg ccgctgcggc tcggcagcgc gcgccgcggc
     8701 ctcgtgcccg tagccatcct cggcggcgcg atggcgctga ccgcgatcgg ctgggtgccg
     8761 gtcgcgatcg ccttcttcgc cgccgccgcg ctgatcatgc tcgccggcgc gctccccgcc
     8821 cgcgaggcct acagccacat cgaatggccg atcctggtga tgatcggcgc gcttatcccg
     8881 gtctccgatt cgctgcgcac caccggcgcc accgacctca tcgccgcctg gctctccgcc
     8941 accgccgcca ccctccccgc ctgggccgcg gtcgccttga tcctggtcgc cgccatggcg
     9001 gtgacgccgt tcctcaacaa cgccgccacc gtcctcgtca tggctccgat cgccgccacc
     9061 ttcgcgaccg agctcggcta tcgccctgaa gccttcctga tggcggtggc ggtcggcgcc
     9121 ggctgcgatt tcctcacgcc gatcggccac cagtgcaaca cgctggtcat gggcccgggc
     9181 ggctaccgct tcggcgatta cgcccgcctc ggcgcgccct tgtcgctgct ggtggtgctg
     9241 gtcggcgtgc ctttgatcct cgccgtctgg ccgctccgct actagccccg cggctcaagc
     9301 cccccacacc tcgcgcgcat aagcgaggaa attgagcccc atgatcttct ccacccggcg
     9361 ctccgaatag ccgcgccgcc gcagccgctc ggcaaggtcg cgaaactggc tcggcccctg
     9421 caattcctcg acgaacggca aggtgtccgg attctcgcct ttggcgccga tcccagcctt
     9481 gcgccgctct tcgatctccc cggcgatcag ccggcgataa ccggccatgt cgtcgacccg
     9541 ggtgaggccg ccgtcggtgc cgatcccgac atggtcctcg ccgcacacct tcaccgcatg
     9601 gtcgatatgc gcgaccacat cctcggcggt ggcgacgctg tcggggctga ggaagggcat
     9661 gaaatagatg ccgacgaagc cgccccgctc cgccaccagc cgcaattccg cgtcgctctt
     9721 gttgcgcggc aggtcggtca gcgcccggca gccggtatgg ttgatcgaga tcggctggcg
     9781 cgacgcccgc gcggcttcga ggcaggtgcg ctcgccgcta tgcgacaggt cgaccatgat
     9841 gcgctgcgcg ttgagccgct cgaccagttt gcgccccagc ggcgtgagcc cgcgattgag
     9901 cggcgccatc gcgccgtcgc cgatcgaatt ggcgggattg taggtgagct ggattacgcg
     9961 gacgccaagg tcggcgaata tctccacccg gtccggcctg gcgcccatca tcgccgcatt
    10021 ctggaagccg tagatgatgc cgatcttgcc ctcggccctg gcgcgcggga tgtcggcggc
    10081 ggtcagcacc ttcagcaaat cgccgggatg cgcgcgcagc cgccggtccc acaggccaat
    10141 gtcgcggacg ctatattcga acggctccat gtcgccggag acatagccga gcgtgcagtt
    10201 gatcgccgtc acgcccgagc cccgcgcgtc cgcgatcacc cgcgcctcga acccgggccg
    10261 ctcggctccc cagtccttgc tgggattggg attggcgagc ccgcccagcg cattgacgat
    10321 gatcatgtcc ggagcggccg gtgccgcccg caccagcgac ggcatcgccg ccgcggcccc
    10381 gcccgccatc gccaggaagt agcggcgatc gaccgctgtc accaaggcgg acctccccct
    10441 tcggcgagcc cctcaggccg cccgtgcgag gaacgcgctc tcccgcgcca cccacggcgc
    10501 gaagctcggc cgcgccagca tcccctcgat ataggccatc agcttcggcc gcttgccggc
    10561 gccgcagtcg agcccgagat ggcggaaatt ggcgaacggg ctcgccaccg cgatgtcggc
    10621 cagcgtcagc cggtcctcga ccaggaagcc gctgtcgggg atcacccgtt ccagatagtc
    10681 gaggatcggc ggcagctcct cgcactcggc tttctccgcc accgccagat cgccctcgcg
    10741 cttcaggaat ctgggcgcga ccacgcggtt gaagaacatc ttggtgccgc acgcgccgac
    10801 gatggtgtcg gcgaattcgt cgaaccagat cgcccgcgcc cgcgccttgg cctccgccgg
    10861 gatcaggttc ggctcgggct tcaccgcctc gagataggcg acgatcgcgc tcgaatcgga
    10921 gatggcgaag tcgccgtcgc gaaagccggg gatcttccgg aacgggctcg actcgacgaa
    10981 ttcgggctcg tccgaccata aggcgaccgg cttcgattcg acctcgatcc ccttctcggc
    11041 ggcgaaggcc agcaccttgc gcacgaacgg cgacagcgac gaaccataaa cgatcatgcc
    11101 ggcttctccc tggtggcccc gcccccggat agccgcgccc gcccccccgc tcaacccgcg
    11161 cggcgcaggc gcgaagcgca tcggtgacag ccccccgccg acccgctacc aaatcgcgca
    11221 tgagttcctc cgccccctcc ccctccgccg aggccttgat cgtgcggccc gtcgaggacc
    11281 cggccgatcg caaggcgttc gtcgacgtcg cctggactct gaattccggc gatccgaact
    11341 gggtgccgcc cttgaagacc gaggtccacg gcctgctcga tccgcgcacc aacccctggt
    11401 tcgaacatgc cgaggcgcgc ctgttcctgg ccgagcgcgg cggccgcgcc gtcggccgca
    11461 tctcggccca ggtcgacagc ctcgtccagg cgatgccgca gcatcagggc ggcggcgagg
    11521 gcaccggcca ttggggtatg ttcgaagccg ccgacgccga ggtcgcggcc gccttgatcg
    11581 cgcgggccga ggatttcctg cgcggccgcc gcatgcgccg cgcgatgggc ccgatcagcc
    11641 tctccatctg ggacgagccc ggcctcctgg tcaaaggcca cgatcatccg ccgaccgtga
    11701 tgatgggcca aaacagtccc gcgtacgaag cctggatcga ggccgccggc tatcgccagg
    11761 cccgcgacct cctcacctac gagctcgaca tcactcagac catgccgccg ctcgtccagc
    11821 gcatcgtcgc cagcggcgag cgcaacccgc gcatccacgt ccgcaaggtc gacaagtcgc
    11881 gcttcgacga ggaagccgcc ctgatcctcg gcatcttgaa cgacgcctgg agcggcaatt
    11941 ggggcttcgt gccgctgacc gacgccgaaa tcgcttatgc cggccgcaag ctcaagccga
    12001 tcgtgcgcga ggatctgatc cgcgtcgccg aggtcgacgg cgatccggtc gccttcatga
    12061 tcaccctgcc cgatctcaac gaattcaccc gcgatctcaa tggccgcctg ttcccattcg
    12121 gctgggcgaa attgctgtgg cggctccgca accccaaggt gaagacgatg cgcgtgcctt
    12181 tgatgggcgt cgtcaagcgg ctccagtcga cccggctcgc cagtcaactc gctttcatgc
    12241 tgatcgaata tatccgccgc tcctcgatcg ccgactatca cgccactcgt ggcgagatcg
    12301 gctggatcct cgacgacaac cacgccatgc gcgcgatcgc cgagaccatc gaaagcagca
    12361 tcaaccgcgt ctaccgcatc tacgaaaaac cgctctcgta gcgcggaatt gttccccggc
    12421 gaaagccggc gcccgttccc cggcgaaggc cggggcccag tcccccggcg aaagccgggg
    12481 tccagcggac ggccaaagct ggaccccggc cgtcgccggg gaacgacccc tcagcgccgc
    12541 tccgcccgcg ccttcgcggc aagcgcgcgc agccgccggg tggtggcgcc gtcccccgaa
    12601 agcgccgccg ccagccggat ctcggtggtc gtcccgtccg cataaagcgt cacccgccgc
    12661 cgcacataat ggccgagctc gggcgcccaa tcccattgca cgcgctcgac cagccgcatg
    12721 gtcgaggccg aatagcgctc gcactggatc ggccaggcct cgaacgtccc cgccggcacc
    12781 gccaccggcg ccggccgccc gacgccgcac ttccagaagg cggccgagcg gcgggcgccg
    12841 cctttgccgt cgacgatcgt caccgccggg aagcgcgccg tcttgccggg cgcgagcggc
    12901 cacagccgcc gcgccgcctc gccgacgtcg cgccgcccgc cgaccccggc caccttccag
    12961 gcgaggatcg gcacgatcgg gttggcgctg cgctcgtagg tggtccggcc gatgcccgac
    13021 caggtcacgc gctcgccgct gacgccgcgc acccgctcga cccgcccgtc attaaacacg
    13081 aaggcgtcgc caggctcgta ggaaggaagt tgggccgcgc ccgcggccag cagcagggcg
    13141 atcatgattc ggggtcgacg agcccatagc catagaccgg gtcgcgcccg ggcgcgccga
    13201 ggtcgcgcgc cgcccgcacc atccccgcca ccgcccgctc gcgcgcggcc ggatcgggcg
    13261 cccgcaactg ccgcgccagc cgcgcgctga ccttgggcgc ggcgaacgag gtgccgctga
    13321 cgctgcgcgg cgtgccggtc gaatcgaccg ccgtcacgtc gacgccttcc gccgccagca
    13381 tggtgtgcgg cccgcgattg gcgcgccggt agatggagcc gcgtccgtcg accgcggtca
    13441 cgccgatcac ttcgggataa gcggccggat agagcggcgg cgccgccggc ccgtcattgc
    13501 cgaccgcggc caccaccgca tggccgcgca gccgcaagat cttgatcatg cggtggacga
    13561 tcgggttggg cgggccggcc aggctgatgt tcaccaccgg caccttcttg gccgccatcc
    13621 agtcgagcgc ccgcaccatt gccgcggccg aggcgttgcg gccgtcgaag atgttggccg
    13681 aatagatcgt gccgacgccc gcgtccgcca gcagggcggc caccgccgtg ccgtgatgct
    13741 ggcgcgccga ggcatgcggg gcgaagctca cagcctcgac cttgacgccg gccagcgccg
    13801 ccggcgcgac cccgccgtcg atgatcccga ccgggccgcc gccgcccggc cgccgcgccg
    13861 cccgccgccc gggcggcgtc ttgccgacat tgccggcggc gaaaaggtgg ttgagcgcga
    13921 agatcgaatc cgatttgcgg ctgaccagct gggcgagcgc cgcttcggcg gtgctggttt
    13981 ccggcaccgc gatcttcagc agctcgagcc cggtcccggg cagcgattcg cgcgacgaca
    14041 ctcggaagcc aagagcctgc agcgtgtcgc ggtcgctcgg atcgtcggtc agcgccagca
    14101 cttcgcgcgc cgcgaccgca tagccttcgc gatcatattc caccccccgc gactcgcccc
    14161 cgccgcgccc ggcctcggcg cctccagagc cgtggccgct attgtcatcg tggcgcccgt
    14221 cacgcccgga gccgctgcgc ccgtcgcgca gatcgtcggc cgcatcggcg cgatcgtcgg
    14281 ccgcatcctc ggccgcgtcc gcacggtcgt c
//
LOCUS       DASVRK010000260        17202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_600875, whole genome shotgun sequence.
ACCESSION   DASVRK010000260 DASVRK010000000
VERSION     DASVRK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 17202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17202
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600875"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..2438
                     /locus_tag="VF589_10350"
     CDS             252..2438
                     /locus_tag="VF589_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX8258024.1"
                     /translation="MNNLPVPNRAPLELWGGAECTIVRVGDSWRDQAIETGHKFRRED
                     IDRFAELGIKRVRFPILWEAVAPDRPDEYDFEWQDDRLALLRERGIEVIGCLLHHGSG
                     PHYTELLDPEFPRKLGAYATRVAERYPWIKMWTPVNEPLTTARFSALYGHWYPHTKDY
                     ACFLRALANECKGTLEAMRAIRRFIPDAQLVQTEDLGKCYATAPLRYQANFENERRWL
                     SLDLLCGRIDTRHKMHRLMLGAGIGWDEMEAFAGGEAKPDLLGINHYLTSERFLDHRT
                     GLYPDHEIGGNGRDRYVDAEAVRVKRLDADTGFGPRLREAWARYGIPIAITEVHHGCH
                     RDEQLRWFAEVWETAGTLRDEGMDLRAVTIWSMFGNVDWRFLLTQKNGFYDTGAYDVR
                     GGAPRPTIIAKAGKAYAKGETFDHPVLDAPGWWRRPERLYPWNGRCKGLEGGGRKILI
                     TGATGTLGNAFARICDVRGLPYCLTSRAELDICDDESIRAAIAHHRPWAIINTAGFVR
                     VADAEREREACLSANAAGPEKLARACEEAGLPLVTFSSDLVFDGQLGRPYCETDPVKP
                     ANVYGLSKAKAERNVLGISDHALVIRTSAFFGPWDKYNFAWNTLSALARGEPVEASRN
                     SAVSPTYVPDLCHATLDLLIDGETGIWHLANQGKLSWYEFATQVAEGAGYDPALVVPI
                     EDKPAVTALTSARGIMMRPVDDAIGEYLAGLGDDAPLPRQLDIAAE"
     gene            complement(2414..3652)
                     /locus_tag="VF589_10355"
     CDS             complement(2414..3652)
                     /locus_tag="VF589_10355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8258025.1"
                     /translation="MWLLDRTLKRLVRSGELIVREADGRETRYGTPAPGRAPVAIRFT
                     DRRVVFDLLRDPRLGGGEAYMNGRMVVEEGEILDLLDLFRSNTSWERTPGKKGLRKGG
                     SKAIARLNRLNWERRSRKNVAHHYDLSDRLYDLFLDADRQYSCAYFTDPSVSLDQAQE
                     DKKAHIAAKLYLKPGQRVLDVGCGWGGMALYLNRVAGVDVLGVTLSEEQLKVARRRAE
                     EAGVADRVKFELIDYRNVAGQFDRIVSVGMFEHVGPPHYRAFFRKCRSLLADDGVMLL
                     HTIGRMGPPGTTDRWIQKYIFPGGYNPALSEIVGASERVKLIASDVETLRLHYGYTTE
                     HWYRRVTDKRAEIEALYDERFFRMWQFYLAGAAVTFRHGGMVNYQVQYIRNRRALPII
                     RDYMAEAEARYRSYSAAMSS"
     gene            complement(3719..6079)
                     /gene="glf"
                     /locus_tag="VF589_10360"
     CDS             complement(3719..6079)
                     /gene="glf"
                     /locus_tag="VF589_10360"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX8258026.1"
                     /translation="MISLSTASSLGPDSPVSLPASDHALICFSHLRWNFVFQRPQHLM
                     SRFAAGRRVIYWEEPEAALPDCEPALGVRTCAETGVIVVTPSLPESLDDEARQTALKA
                     LLDDFLGSEDQPLIRWYYTPMMLPFSRHLAAAATVYDCMDELANFRFAPKELLGLERE
                     LFAASDVVFTGGYSLYEAKKGRHPNVHPFPSSVDRAHFAQARATDAAPDDQARLPRPR
                     FGFYGVIDERMDLDMIASLADAHPDWSVVMVGPIVKIDPADLPQRANIHYLGNKKYEE
                     LPHYLGGWDVALMPFAINDSTKFISPTKTPEYLAGGRPVVSTSITDVIRHYGDLEAVF
                     IADGAEAFVAGCEQALALARDDDDWLATVDAKLANLSWDTTYARMAGLLQEAIAVPSA
                     GPRLVTAAGKKKYDYLVVGAGFAGSVIAERLASQHDAHVLVIDKRPHVAGNAYDHLDE
                     AGVLIHQYGPHIFHTNSDEIVDYLSQFTRWRPYEHRVLADVRDQLVPIPINRTTLNKL
                     FGLNLETDEEAAAYLASRAEPVGEIKTSEDVVVSAVGRELYELFFQGYTRKQWGLDPS
                     ELDKSVTSRVPTRTNTDDRYFSDKHNIMPAEGYTAMFNAMLDHPNIDVLLSTDFRDVM
                     DEIEAGHIVYTGPIDEYFDWQYGKLPYRSLRFEHKVVDQEVFQPVAVVNYPHPQVPYT
                     RITEYKYLTGQSHPKTSITYEYPSAEGDPYYPIPRPENQELFKKYEALADATEGVTFV
                     GRLATYRYYNMDQVVGQALAAFRKLDAKRKPSAKAAKRTVWTEAAE"
     gene            complement(6258..8312)
                     /locus_tag="VF589_10365"
     CDS             complement(6258..8312)
                     /locus_tag="VF589_10365"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX8258027.1"
                     /translation="MAKPPPFGAIHRHGFVRTGVATPVASAGDVTLNVRETIELARQG
                     HERGCDLIVFPELNVSSYAVDDLHLQEAFLDAVEQGIADICAATAELGPVLAIGAPLR
                     RNGRLYNCALILARGRILGVVPKSFLPNYREYYEKRWFVSGIGLNGIDIAVAGQNVPF
                     GTDLIFAAEDLADFIFHVEICEDYWAPQPPSTVGALAGALILCNLSASNITIGKADER
                     KLLCASQSSRCCAAYLYAASGPGESTTDLAWDGQGSIYELGELIAESRRFEATRELIA
                     ADIDVQRLRLERMRMPTFNDNAVAAGHPEKTARRIHFEHRPNLADIGFERRYRRFPYV
                     PNRPEQLDQDCYEAFNIQVQGLARRFQATAGSKLVIGVSGGLDSTHALIVAAKVCDYL
                     GLPRDTILGFTMPGFATGEATKANAWALMNALGIVGEEIDIRPAARQMLGDIGHPFAR
                     GEPVYDVTFENVQAGLRTDYLFRLANQRAGFVIGTGDLSELALGWSTYGVGDQMSHYG
                     VNAGVPKTLIQYLIRWVVKSGQFDAATNDILTAILATKISPELVPADEAGEMQSTEDR
                     IGPYELHDFFLFHILRSGQPPSKVAFLAWQAWKDAGEGLWPIDYPDVLKREYDLPTIR
                     RWLEVFLYRFFQISQFKRSALPNSPKVSMGGSLSPRGDWRAPSDGVAGPWLDELKASF
                     DG"
     gene            complement(8313..8813)
                     /locus_tag="VF589_10370"
     CDS             complement(8313..8813)
                     /locus_tag="VF589_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX8258028.1"
                     /translation="MNDTETLSPLLPDETEEATRRLLAKACERDLMFATAESCTGGML
                     ASLLTDVRGVAHAFDRGFVTYTNAAKHEMLGVPLELIDAHGAVSREVAIAMAEGALER
                     SRAHIALSITGFADAGDEPGLVHFGCARAGRRTAHREEHFGPGGRGATRVKCMKVAVE
                     MMSEML"
     gene            8894..9397
                     /locus_tag="VF589_10375"
     CDS             8894..9397
                     /locus_tag="VF589_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8258029.1"
                     /translation="MTSQGKQNAIELLTEDHRTVEALFERFEKAGDGEKEQIAKSICT
                     ELKVHAMLEEEIFYPALRGKIEDDDLDEAMVEHDGAKLLINEIEAGSSDDQYYDAKVK
                     VLQEQIEHHVKEEEKQQGNIFTQARKADVDLEALGEQMATRKAELMQLAETQGLPPAK
                     TATMETA"
     gene            9489..9695
                     /locus_tag="VF589_10380"
     CDS             9489..9695
                     /locus_tag="VF589_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258030.1"
                     /translation="MGGRGEGERGAPALSGDGVPGPVGDSGNVRPAGPDATRDPPKEW
                     TKVDEASDESFPASDPPNFNPHID"
     gene            complement(9711..10223)
                     /locus_tag="VF589_10385"
     CDS             complement(9711..10223)
                     /locus_tag="VF589_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258031.1"
                     /translation="MTKDDPKLGSRLLIGAIAGIVGTMAMTSAMHRLHKKLPPRERYP
                     LTPREIVDQVGAELRAELDSGQAKDVTTAAHFAYGAATGALLGAANVMMGPVTGGLAG
                     MSVWLGSYMGWIPGAGILKPATRHPARRNLLMIGVHVVWGVTTARAMRELVEARATMM
                     RDGPDKDAPG"
     gene            complement(10220..10696)
                     /locus_tag="VF589_10390"
     CDS             complement(10220..10696)
                     /locus_tag="VF589_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEX8258032.1"
                     /translation="MTEYFVLKFLHVIGAAVLLGTGAGIAFFMLLAHRTGDVKVIAGV
                     ARIVVIADYVFTATAVVAQPITGVLLARTVGYDLAEGWIVASIALYVFTGLFWLPVVW
                     MQARMQTLSREAAASGAPLPPEYHRLFRWWFAFGFPAFAAVLLIFWLMITRPEIGF"
     gene            complement(10693..11985)
                     /locus_tag="VF589_10395"
     CDS             complement(10693..11985)
                     /locus_tag="VF589_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8258033.1"
                     /translation="MRILLIGATGFIGSAVAARLAGEGHALVGTARNIDAAARRLPGG
                     LWVELDLRSATTPEAWLRHLAGVDAVVNCAGALQDSPRDSTGRVHRDAPAALWQACAR
                     AGVRRVIHFSAMGVDRGGLTAFSRSKGEGDEALAASGLDWVILRPSVVVGRQAYGGSA
                     LFRALAAFPILPKSPDAGPLDIVQLDDVVETVVRLLQPGAPSGLALELAGPDRLSFEE
                     VVQSYRRWLGWRPARIVAAPGLLMAAAYRAGDLIAKLGWRPPIRSTARREIVRGAVGD
                     PSGWIEATGIRPQSLAAALAAEPASVQERWFANLYLLKPLAIGTFALFWLLTGLISLG
                     PGYDLAANYMRLAGAGPLSEPSVIAGGVADIIVAFGILYRRTARLALYAAILLSLFYV
                     VAGTILLPQLWRDPLGPMMKIWPILAFNFLCLAILDER"
     gene            complement(11996..14500)
                     /locus_tag="VF589_10400"
     CDS             complement(11996..14500)
                     /locus_tag="VF589_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8258034.1"
                     /translation="MWRNYLIVGLRALLRNRSYAFITIFGLAAGMGACMLLLLYVQHE
                     RSYDAWLPNAENIYQVQSHWRDPETGESTDTQMSAYVAGKTLAKDFPQVERVAYAFSA
                     APVVIHRGQALPTEDVVLVDGPFFDMFHFPFSDGDPATALREPGSVVLSESEARKYFG
                     GADAIGETLTLVMQGNRIDHRVTGVMRDLPRNSHMRLAMVARFDPGSYFAESPEFLTA
                     WGWYQGWNYLALRPGTDPAAIAAQLPAWEKRNIPDDTSDGGRTNFGDEGDWSLANIRD
                     VHLGDVTEGAMSPGNDRRTIVTFVAIAFLILAMAVVNFINLTTARAGQRAREVALRKV
                     LGATRRQLMLQFLVESVLIAGLAMLVALAIVELSLPWLSAQLGAELQTHYIGAEGMLP
                     TMLLLTLAVGLLGGLYPAFYLSRFQPAEVLKANKSSAEAAGAGRLRAALVVGQFAVSI
                     GLIICTVVVYAQTLHALSADPGYRREGILQIAELDRRQLVDRSEAIAREIARVPGIRA
                     VGRTGIGIGADNQLANQVHVPGRAQPAAIGSYMIDDGFFRTMEMKLLAGRPLDRARPL
                     DADPRGYPSDLAQDRALAGRGLNVVVNALAARQLGFNQPTDAVGAQLRMDIAEEAAGL
                     VPVTIVGVVDNARLRSIRDTIEPTIFRLGESELTHLVARYEGAEPREVRDRVEQTWRR
                     LVPDVPFNAAFGEEIVAELYDAEEARAQTFAGFATIAVIIACLGLFALAAFTAERRTK
                     EIGIRKVLGANTRDIVRLLAVQMLKPVVLANLIAWPVAWWVMRDWLNTFDSRIALGPG
                     PFIVAGLAALAIAFGTVAGHALKVARANPIRALRYE"
     gene            complement(14610..14840)
                     /locus_tag="VF589_10405"
     CDS             complement(14610..14840)
                     /locus_tag="VF589_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258035.1"
                     /translation="MIMNEFVWAGAALAVTGTIAFAFLRAWRGWLELKRYEIAARHGG
                     LGEGGGSGARIELADVRERLRKLEAIATGVDP"
     gene            complement(14842..>17202)
                     /locus_tag="VF589_10410"
     CDS             complement(14842..>17202)
                     /locus_tag="VF589_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8258036.1"
                     /translation="LPNAENVYQVHSTYTDPDTGERGGFQMSPYPVGATMKKDFPQID
                     KLAYTFAGQAVAYRGGEVSTVDDTLYTDGNLLDVIELPLLKGDPARALTQANSVVLTE
                     SEAHRQFGDADPIGRTLTLVSRGEHVDHRVTGLMPDLPKNSHLRANMIVRFDPARVFA
                     DEPNYASEWGNTNGWNYVSLRPGTRLEDIHAEMPAWERRNIPTQTFGGETINVGENQD
                     WHLVNVRDIHLAQQQGGAMSEGNDRRTILTFTVVALLILGMACVNFTNLATARATQRA
                     REVGLRKVVGATRRQLIVQFLGESVLLVSIAMLVALALVELLLPALGRFLDADLRLTY
                     LGQKGVLLPILAMVLVVGAIGGLYPAFYLSRFQPARVLKANKSAAETPGAGYLRSALV
                     VAQFAVSIGLIICTAIVYSQTMYARTADPGYKREGVLQISGIARKQMVALGETLVREV
                     ERVEGVTAAARTSIGVSTPSNMNRNSYVPGRDEGVSIGNYPIDAGFFEVMGIKRVAGR
                     LLDRNRPLDDTSVPDTAPLEQKQAMAARGGNVVINELAARRLGFANPADAVGATVRTA
                     IFFNAPEAGLVPLTIVGVVEDSRFRSVRQPIDPIMYRLSGDYLSHLVVRYNSAEPNAV
                     RDRIEAVWKRLAADVPFEARFSEDIVAELYDAEEARAKTFGGFALLAMVVACLGLFGL
                     AAFAAERRTKEIGIRKVLGARSRDIVRLLAWQFSKPVIVANLLAWPIAWWVMRDWLNS
                     FDTRIALGATPFLLAGLIALAIAIGTIAGHALKVARANPIHALRYE"
BASE COUNT         2954 a         5405 c         6179 g         2664 t
ORIGIN      
        1 aggcgcgctg ggacaaggac ggcgacgcgc gctggggcca ttatgcgcgc atctggcaga
       61 agcggagcga gggctggcga ctaatcgtcg acctgctgat cccggcgcca ggaaaccccc
      121 cgactgaacc ggaaggtgcg gcaacgaaag cgaaataatg gctaacctgc tttagcaacc
      181 gcggaaaact gcggaactgc ccacatcaca agccgttcgt taccaaaccg ttaggaaccg
      241 aacagctgat tatgaacaac ttgcccgtgc caaaccgggc tccgctggag ctttggggcg
      301 gggccgaatg cacgatcgtt cgcgtcggcg acagctggcg cgaccaggcg atcgagaccg
      361 gccataaatt ccgccgcgag gatatcgacc gcttcgccga actcggcatc aagcgcgtcc
      421 gcttcccgat cctgtgggag gcggtggcgc cggaccggcc ggacgaatat gatttcgaat
      481 ggcaagacga ccggctggcg ctgctgcgcg agcgcggcat cgaggtgata ggctgccttc
      541 tgcaccacgg ctcgggcccg cattatactg agctgctcga ccccgaattc ccgaggaaac
      601 tcggcgctta tgcgacgcga gtggcggagc gctatccgtg gatcaagatg tggaccccgg
      661 tcaacgagcc gctgaccacg gcgcgcttct cggcgctcta cggccattgg tatccgcaca
      721 ccaaggatta tgcctgcttc ctccgcgcgc tggccaacga gtgcaagggc acgctggagg
      781 cgatgcgcgc gatccgccgc ttcatccccg acgcccaact ggtccagacc gaggatctcg
      841 gcaaatgcta tgcgaccgcg ccgctgcgtt accaggccaa tttcgagaat gagcggcgct
      901 ggctgagcct cgacctgctt tgcggccgga tcgacacgcg ccacaagatg caccgattga
      961 tgctcggcgc cggcatcggc tgggacgaga tggaagcctt tgccggcggc gaagccaagc
     1021 ccgacctgct cggcatcaac cattatctca ccagcgaacg cttcctggac caccgcaccg
     1081 gcctctatcc ggaccatgag atcggaggca acgggcgcga ccgctatgtc gatgccgagg
     1141 cggtgcgggt gaaaaggctc gacgccgaca ccggtttcgg gccccgcctg cgcgaggctt
     1201 gggcgcgcta cggcattccg atcgcgatca cggaagtcca tcacggctgc caccgcgacg
     1261 agcaattgcg ctggttcgcc gaggtctggg agacggccgg cacgcttcgc gacgagggca
     1321 tggacctgcg cgccgtcacc atctggtcga tgttcggcaa tgtcgactgg cgctttctgc
     1381 tgacccaaaa gaacggcttc tacgacaccg gcgcctatga cgtgcgcggc ggggcgccgc
     1441 ggccgacgat catcgccaag gccggtaagg cctatgcgaa gggcgagact ttcgaccatc
     1501 cggtgctcga cgcgcccggc tggtggcggc ggcccgagcg gctctacccg tggaacggcc
     1561 ggtgcaaggg gctggaaggc ggcggccgca agatcctgat caccggcgcg accggcacgc
     1621 tcggcaacgc cttcgcccgc atctgcgacg tacgcggcct gccttattgc ctgaccagcc
     1681 gggccgagct cgacatttgc gacgacgaat cgatccgcgc cgctatcgcg catcaccggc
     1741 cctgggctat catcaacacc gccggcttcg tccgcgtggc cgatgccgag cgcgagcgcg
     1801 aagcctgcct cagcgccaat gcggccggac cggaaaagct cgcccgcgcc tgcgaggaag
     1861 ccggcctgcc gctggtcacc ttctcctcgg acctggtgtt cgacggccag ctcggccggc
     1921 cgtactgcga gaccgatccg gtcaagcccg ccaacgtcta cgggctcagc aaggccaagg
     1981 ccgaacgcaa cgtgctcggc atttcggacc atgcgctagt gatccgcacg agcgccttct
     2041 tcgggccgtg ggacaaatat aatttcgcct ggaacacgct gagcgcgctc gcccgcggcg
     2101 agccggtcga ggccagccgc aactcggccg tgtcaccgac ctacgtgccc gatctgtgcc
     2161 atgcgacgct ggatctgctg atcgacgggg agaccggcat ctggcacctc gccaaccagg
     2221 gcaagctcag ctggtacgaa ttcgcgaccc aggtcgccga aggcgccggc tacgaccctg
     2281 ctctggtggt gccgatcgag gacaagccgg cggtgacggc gctgaccagc gcgcgcggca
     2341 tcatgatgcg gccggtggac gatgcgatcg gcgagtatct ggccggcctc ggcgacgatg
     2401 cgccgctgcc gcgtcagctg gacatcgccg ccgaatagga acggtagcgc gcctcggcct
     2461 ccgccatata gtcgcgaatg atggggagcg cgcggcggtt gcggatatat tggacctggt
     2521 aattgaccat gccgccatgg cggaaggtga ccgcggcgcc ggccaggtag aattgccaca
     2581 tgcggaagaa gcgctcgtcg tagagcgctt cgatctcggc gcgcttgtcg gtgacgcggc
     2641 gataccaatg ttcggtcgta tagccgtaat ggagccgcag cgtttcgacg tcggaagcga
     2701 tcagcttgac ccgctcgctg gcgccgacga tctccgacag cgccggattg tagccgcccg
     2761 ggaagatgta tttctggatc cagcggtcgg tggtgcccgg cggccccatc cggccgatcg
     2821 tgtggagcag catgacgccg tcgtcggcga gcaggctgcg gcatttgcgg aagaaggcgc
     2881 ggtagtgggg cggcccgaca tgttcgaaca tgccgaccga gacgatccgg tcgaactgcc
     2941 ccgcgacgtt gcgatagtcg atcagctcga acttgacgcg gtcggccacg cccgcttcct
     3001 cggcgcggcg gcgggcgacc ttcaactgct cttcggagag ggtgacgccg agcacgtcga
     3061 cgccggcgac gcggttcaga taaagcgcca tgccgcccca gccgcagccg acgtcgagca
     3121 cgcgctggcc cggcttgagg taaagcttgg cggcgatatg cgccttcttg tcctcctggg
     3181 cctggtcgag gctcaccgaa ggatcggtga aataggcgca gctatattgg cggtcggcgt
     3241 cgaggaagag atcgtagagc cggtcggaaa ggtcgtaatg gtgggcgacg ttcttgcgcg
     3301 agcgccgctc ccaattgagc cggttgagcc gcgctatggc cttgctgccg cccttgcgca
     3361 ggcccttctt gccgggcgtg cgctcccacg acgtgttgga gcggaacaga tcgaggagat
     3421 cgagtatctc gccctcttcg accaccatgc gaccgttcat ataggcctcg ccgccgccga
     3481 gccgcgggtc gcgcagcaga tcgaaaacga cgcggcggtc ggtgaagcgg atagcgaccg
     3541 gcgcccgccc cggtgcgggc gtgccgtagc gggtctcgcg tccgtcagcc tcgcggacga
     3601 tcagttcgcc ggagcggacc aggcgtttca gggtacggtc gagcagccac atgccgcccg
     3661 cttacgcaaa aacgggcgac aggcaagagc gtgaggagtc cggccggagc cgaggcggct
     3721 attcggccgc ttcggtccag acggtgcgct tggcggcctt ggccgagggt ttgcgctttg
     3781 cgtcgagctt gcggaaagcg gcgagcgcct gcccgacgac ctggtccata ttatagtagc
     3841 ggtaggtcgc gagccggccg acgaaggtga cgccctcggt ggcgtcggcg agcgcttcgt
     3901 acttcttgaa cagctcctga ttctccggcc gcgggatcgg ataataagga tcgccttccg
     3961 cgctcgggta ttcgtaggtg atgctggtct tcggatgcga ctggccggtc agatacttat
     4021 actcggtgat ccgggtataa gggacctgtg ggtggggata gttcaccacc gcgaccggct
     4081 ggaacacttc ctggtcgacc accttatgct cgaagcgcag gctgcgatac ggaagcttgc
     4141 cgtattgcca gtcgaaatat tcgtcgatcg ggccggtata gacgatgtga ccggcctcga
     4201 tctcgtccat cacgtcgcgg aaatccgtcg acagcagcac gtcgatgttc gggtgatcga
     4261 gcatcgcatt gaacatcgcc gtgtagccct cggccggcat gatgttgtgc ttgtccgaga
     4321 aatagcggtc gtcggtgttg gtgcgggtcg ggacgcgcga ggtcaccgac ttgtcgagct
     4381 cgctcggatc taggccccat tgcttgcggg tatagccctg gaagaacagt tcgtagagct
     4441 cgcgcccgac cgcggagacg accacgtcct cggacgtctt gatctcgccc accggctcgg
     4501 cgcgcgaggc gagataggcg gcggcctcct cgtcggtctc gaggttgagt ccgaacagtt
     4561 tgttgagcgt ggtgcggttg atcgggatcg gcacgagctg atcgcggacg tcggcgagca
     4621 cgcgatgctc gtaggggcgc cagcgggtga attgcgacag atagtcgacg atctcgtccg
     4681 aattggtgtg gaagatgtgc ggcccatatt ggtggatcag cacgccggct tcgtcgagat
     4741 ggtcataggc attgccggcg acgtgcgggc gcttgtcgat gaccagcacg tgggcgtcgt
     4801 gctggctggc aagccgctcg gcgatcaccg agccggcgaa gcccgcgccg acgaccagat
     4861 aatcatattt cttcttacca gcggcggtga ccagccgcgg gccggcgctc gggacggcga
     4921 tcgcttcctg gagcaggcct gccatgcggg cataggtggt gtcccagctg agattggcga
     4981 gcttggcgtc gaccgtggcg agccagtcgt cgtcgtcgcg ggccagcgcc agcgcctgtt
     5041 cgcagccggc gacgaacgcc tcggcgccgt cggcgatgaa caccgcctcg aggtcgccat
     5101 aatggcggat gacgtcggtg atcgacgtcg atacgaccgg gcggccgccg gcgagatatt
     5161 cgggtgtctt ggtcggcgag atgaacttgg tcgagtcgtt gatcgcgaac ggcatcaagg
     5221 cgacgtccca gccgccgaga taatgcggca gctcctcata tttcttgttg ccgagataat
     5281 ggatgttggc gcgctgcggc agatcggccg ggtcgatctt gacgatcggg ccgaccatca
     5341 cgaccgacca gtcggggtgg gcgtcggcga ggctcgcgat catgtcgagg tccatgcgct
     5401 cgtcgatcac gccgtagaag ccgaagcgcg ggcgcggcag gcgggcctgg tcgtcgggcg
     5461 cggcgtcggt ggcgcgggcc tgcgcgaaat gggcgcggtc gacgctcgac gggaatgggt
     5521 ggacgttggg gtggcgcccc ttcttggcct cgtagaggct gtagccgccg gtgaacacca
     5581 cgtccgaggc ggcgaacagc tcgcgctcta ggccgagcag ctccttcggc gcgaagcgga
     5641 aattggcgag ctcgtccatg cagtcataga cggtggccgc cgccgcgagg tgccgcgaga
     5701 agggcagcat catcggcgtg taataccagc ggatcagtgg ctgatcctca ctgccgagga
     5761 agtcatcgag cagtgccttg agcgcggtct ggcgggcctc gtcatcaagg ctttcgggca
     5821 gcgagggggt gaccacgatc acgccggtct cggcgcaggt gcgcacgccc agcgcgggct
     5881 cgcaatccgg cagcgccgct tcgggctctt cccaatagat gacgcgacgc ccggccgcga
     5941 accggctcat caggtgctgg gggcgctgaa agacgaaatt ccaccgcaga tgcgagaagc
     6001 aaatcagcgc atggtcggac gccggaaggg acacaggact atcgggcccc agcgacgagg
     6061 cggtggaaag gctgatcaat gtaaacccct gatggttgaa aggcttccga ttaacgtcaa
     6121 agggccgtaa cgagttccgc cccggcgggt ctaactcgat gatccacgtt aatcggggag
     6181 caaggaaagc gccgaaacgg gggattcccg agcccgttta tcgctgcact gcggcatcgc
     6241 acatgcagca tgaattgtca cccgtcgaag gacgccttca attcgtccag ccaggggccg
     6301 gcgacgccgt ccgagggggc gcgccagtcg ccgcgcgggc tgaggctgcc acccatcgac
     6361 actttgggcg agttgggcag cgccgagcgc ttgaactggc tgatctggaa gaagcggtag
     6421 aggaaaacct ccagccagcg ccggatcgtc ggcaggtcgt attcgcgctt cagcacgtcg
     6481 ggatagtcga tcggccacag cccctcgccg gcatccttcc aggcctgcca ggcgaggaag
     6541 gcaactttcg acggcggctg gccggagcgc aggatgtgga acaggaagaa atcgtgcagc
     6601 tcgtatgggc cgatccggtc ctcggtcgac tgcatctcgc cagcctcatc ggcggggaca
     6661 agctcgggcg aaatcttggt ggcgaggatg gcggtgagga tgtcgttggt ggcggcgtcg
     6721 aactggcccg atttcaccac ccagcggatc agatactgga tcaaggtctt ggggaccccc
     6781 gcattcacgc cataatggct catctgatcg ccgacgccgt aggtcgacca gccgagcgcg
     6841 agctcggaga ggtcgccggt gccgatgacg aagccggcgc gctggttggc aaggcggaac
     6901 agatagtcgg tgcgaaggcc cgcctgcacg ttttcgaagg tgacgtcgta gaccggctcg
     6961 ccgcgcgcga agggatggcc gatatcgccg agcatctggc gggcggccgg ccggatgtcg
     7021 atctcctcgc cgacgatgcc gagcgcgttc atcagcgccc aggcattggc cttggtcgcc
     7081 tcgccggtgg cgaagccggg catggtgaag ccgaggatgg tgtcgcgcgg caggccgaga
     7141 tagtcgcaga ccttggccgc gacgatcagc gcgtgggtcg aatcgagccc gcccgagacg
     7201 ccgatcacga gcttcgagcc cgccgtcgcc tggaaacggc gggcgaggcc ctggacctgg
     7261 atgttgaagg cttcgtagca atcctggtcg agctgctccg ggcgattggg gacgtagggg
     7321 aagcggcggt agcggcgctc gaagccgatg tcggcgaggt ttgggcggtg ctcgaaatgg
     7381 atgcggcgcg cggtcttttc ggggtggccg gcggcgacgg cattgtcgtt gaaggtcggc
     7441 atccgcatgc gctcgagccg gaggcgctgg acgtcgatgt cggctgcgat cagttcgcgg
     7501 gtggcctcga atcggcgcga ttcggcgatg agctcgccaa gttcgtagat cgagccctgt
     7561 ccgtcccagg cgaggtcggt ggtgctctcg cctggcccgg aggcggcgta gagataggcc
     7621 gcgcagcagc gcgagctttg cgaggcgcag agcaatttgc gctcgtcggc cttgccgatg
     7681 gtgatgttgg aggcggagag gttgcacagg atgagcgcgc cggcgagcgc gcccaccgtc
     7741 gagggtggct gcggcgccca ataatcctcg catatctcga cgtggaagat gaagtcggcg
     7801 aggtcctcgg cggcgaagat gaggtcggtg ccgaagggga cattctggcc ggcgacggcg
     7861 atgtcgatgc cgttgaggcc gatgccggag acgaaccagc gcttctcgta atattcgcgg
     7921 taattgggga ggaagctttt cgggaccacg ccgaggatgc ggccgcgcgc gagtatgagc
     7981 gcgcaattgt agagccggcc gttgcggcgc agcggcgcgc cgatcgcgag caccggcccc
     8041 agttccgcgg tggcggcgca gatgtcggcg atgccctgct cgacggcgtc gaggaaggct
     8101 tcctggaggt ggagatcgtc gaccgcgtag gaggagacgt tcaattccgg aaagacgatg
     8161 agatcgcagc cgcgttcgtg gccctgccgg gcgagctcga tcgtctcgcg gacgttgagc
     8221 gtcacgtcgc cggcggacgc gaccggcgtg gcgacaccgg tgcggacgaa gccgtggcga
     8281 tggatcgcgc cgaagggcgg cggcttggcc atctagagca tctcgctcat catttcgacg
     8341 gcgaccttca tgcacttgac ccgggtcgcg ccgcggccgc ccggtccgaa atgctcctcg
     8401 cgatgcgcgg tcctgcgccc ggcccgggcg cagccgaaat ggacgaggcc cggctcgtcg
     8461 ccggcatcgg cgaagccggt aatcgacagc gcgatatggg cgcgcgagcg ttccagcgcg
     8521 ccttctgcca tggcgattgc gacctcgcgc gagacggcgc catgcgcgtc gatcagctcc
     8581 agcggcacgc ccagcatctc gtgcttggcc gcgttggtgt aggtgacgaa gccgcggtcg
     8641 aaggcgtggg cgacgccgcg gacgtcggtc agcagcgagg ccagcatgcc gccggtgcag
     8701 ctctccgcgg tggcgaacat caggtcccgc tcgcaggcct tggcgagcag ccgccgggtc
     8761 gcctcctcgg tctcgtcggg cagcagcggg gacagggtct cggtatcgtt cacgtcgttc
     8821 ctcgaaccgg cttttcccgg cgggaaccgg tcccttgccc ggaatgttcc agaggcacaa
     8881 caggaggcat agaatgacga gccaaggcaa gcagaacgcg atcgagcttc tcaccgagga
     8941 ccaccgcacg gtcgaggcgc tgttcgagcg gttcgagaag gccggcgacg gcgagaagga
     9001 gcagatcgcc aaaagcatct gcaccgagct caaggtccat gccatgctcg aggaggagat
     9061 tttctacccc gcattgcgcg gcaagatcga ggatgacgat ctcgacgagg caatggtcga
     9121 gcatgacggc gccaagctcc tgatcaacga gatcgaggcg ggcagctccg acgaccagta
     9181 ttacgacgcc aaggtcaagg tgctgcagga gcagatcgag catcacgtca aggaggaaga
     9241 gaagcagcag ggcaacatct tcactcaggc gcgcaaggcc gatgtcgacc ttgaggcgct
     9301 tggcgagcag atggcgacgc gcaaggccga gctgatgcag ctggcggaga cgcaagggct
     9361 gccgccggcg aaaaccgcga cgatggaaac ggcctgacgc catggccgag tggaaccgca
     9421 agaccgcgct catcaccggc gtcggcgccg cctgcgcggc ggctttgggc tatctggtca
     9481 cgcggcggat gggcggccgc ggcgaaggcg agcgcggcgc cccggcgctc agtggcgacg
     9541 gcgttccggg cccggtaggc gattcgggca atgtccgccc ggcggggccc gacgcgacgc
     9601 gcgacccgcc gaaggaatgg accaaggtcg acgaagcatc ggacgaaagc ttcccggcca
     9661 gcgatccgcc caatttcaac ccgcacatcg actgagcggg accgaacgat ttagccggga
     9721 gcatccttgt cgggcccgtc gcgcatcatc gtcgcgcggg cctcgaccag ctcgcgcatc
     9781 gcccgagccg tggtgacgcc ccagacgaca tggactccga tcatcagcag gttacggcgc
     9841 gccgggtggc gggtggcggg cttgaggatg ccggcgccgg ggatccagcc catatagctg
     9901 ccgagccaga cgctcattcc agcgaggccg ccggtcaccg gccccatcat cacattggcc
     9961 gcgccgagca gcgcgccggt cgcggcgccg taggcgaaat gggcggcggt ggtgacatcc
    10021 ttcgcctggc cggaatcgag ctcggcacgg agttcggcgc cgacctggtc gacgatctcc
    10081 cgcggcgtca gcggatagcg ctcccgcggg gggagttttt tgtgcaggcg gtgcatcgcc
    10141 gaggtcatcg ccatggtgcc gacgatgccg gcgatggcgc cgatcaggag gcggctgccg
    10201 agcttggggt cgtccttggt caaaagccta tctccgggcg ggtgatcatc agccagaaga
    10261 tcagtagcac ggccgcgaag gcagggaagc cgaacgcgaa ccaccagcgg aacaggcggt
    10321 gatattcggg cggcagcggc gcgcccgaag ccgccgcctc gcgcgacagg gtctgcatcc
    10381 gcgcctgcat ccacaccacc ggcagccaga acaggccggt gaagacgtag agcgcgatcg
    10441 acgcgacgat ccagccttcg gcgaggtcgt agccgacggt gcgcgcgagg agcacgccgg
    10501 tgatcggctg cgccaccacg gcggtggcgg tgaagacata gtcggcgatg acgacgatgc
    10561 gcgcgacgcc ggcgatcacc ttcacgtcgc cggtgcggtg ggcgagcagc atgaagaagg
    10621 cgatgccggc gccggtgccg agcagcacgg ccgcgccgat gacgtggagg aacttgagga
    10681 cgaaatattc ggtcatcgct cgtccaggat ggcgaggcac aggaagttga aggcgaggat
    10741 cggccagatc ttcatcatcg gccccaatgg atcgcgccat agctgcggca gcaggatcgt
    10801 gcccgcgacc acgtagaaga gcgacagcag gatcgccgca tacagcgcga ggcgggcggt
    10861 gcggcggtac aagatgccga acgcgacgat gatgtcggcg acgccgccgg cgatgacgct
    10921 tggctcgctg agcggcccgg cgcccgccag ccgcatgtaa ttggcggcga gatcgtagcc
    10981 ggggccgagc gagatcaggc cggtgagcag ccagaacaag gcgaaggtcc cgatcgccag
    11041 cggcttcagc agatagaggt tggcgaacca gcgctcctgg accgaggcgg gctcggcggc
    11101 gagcgcggcg gcgagcgatt gcgggcggat gccggtggcc tcgatccagc ccgagggatc
    11161 gccgacggca ccgcgcacga tctcgcgccg ggcggtgctg cggatcggcg ggcgccagcc
    11221 gagcttggcg atcaggtcgc ccgcgcgata ggcggcggcc atcagcagcc cgggcgcggc
    11281 gacgatgcgg gccggacgcc agccgagcca gcggcggtag gattgcacga cctcctcgaa
    11341 gctcaatcgg tccggcccgg ccagctccag cgcgaggccg ctcggcgcgc cgggctggag
    11401 caggcggacg acggtctcga cgacgtcgtc gagctggacg atgtcgagcg ggcccgcatc
    11461 ggggcttttc ggaaggatcg gaaaggcggc caaggcgcgg aacagggcgc tgccgccata
    11521 agcctgccgc ccgacgacga ccgaagggcg caggatcacc cagtcgaggc cgctcgcggc
    11581 gagcgcctca tccccctccc ctttgctgcg cgagaaagcg gtgaggccgc cgcggtcgac
    11641 gcccatcgcc gagaaatgga tgacccgccg cacgccggcg cgcgcgcagg cctgccacaa
    11701 ggcggcgggg gcatcgcggt ggacgcggcc ggtgctgtcg cgggggctgt cctgcagcgc
    11761 gcccgcgcaa ttcaccaccg cgtcgacgcc ggcgagatgc cgcagccatg cctcgggggt
    11821 ggtggcggag cgcaggtcga gctcgaccca gagccccccc ggcagccgcc gcgcggcggc
    11881 gtcgatattg cgggccgtgc cgaccaatgc atggccctcc cccgccagcc gcgccgcgac
    11941 ggccgatccg atgaagccgg tcgcgccgat cagcaggatg cgcacggaat accgcctatt
    12001 cgtacctgag cgcgcggatc ggattggccc gcgcgacctt gagcgcgtgc ccggccacgg
    12061 tgccgaaagc aatggcgagc gcggccaggc ccgcgacgat aaacggcccc ggccccaatg
    12121 cgatccggga atcgaacgta ttcagccagt cgcgcatcac ccaccaggcg accggccagg
    12181 cgatgaggtt ggcgagcacc accggcttca gcatctgcac ggcgagcagg cggacgatgt
    12241 cgcgggtgtt ggcgcccagc accttcctga tgccgatctc cttggtgcgg cgctcggcgg
    12301 tgaaggcggc cagcgcgaac agccccaggc aggcgatgat gacggcgatc gtggcgaagc
    12361 cggcgaaggt ctgcgcgcgc gcttcctcgg catcgtagag ttcggcaacg atctcctcgc
    12421 cgaaggcggc gttgaacggc acgtcgggga cgagccggcg ccaggtctgc tcgacgcggt
    12481 cgcgcacctc gcgcggctcg gcgccttcat agcgggcgac gaggtgggtg agctcggact
    12541 cgccgagccg gaagatggtc ggctcgatcg tgtcgcggat cgagcgcagg cgggcattgt
    12601 cgaccacgcc gacgatggtg accgggacca gcccggccgc ttcctcggca atgtccatgc
    12661 ggagttgcgc gccgaccgcg tcggtcggtt ggttgaagcc gagctgccgc gccgccagcg
    12721 cgttcaccac cacgttgagg ccgcggccgg cgagcgcgcg atcctgggcg agatcggacg
    12781 ggtagccgcg cggatcggca tcgagcggcc gggcgcggtc gagcggccgc ccggcgagca
    12841 gcttcatctc catggtccgg aagaagccgt cgtcgatcat gtagctgccg atcgcagccg
    12901 gctgcgcgcg gccgggcaca tggacctggt tggcgagctg gttgtcggcg ccgatgccga
    12961 tgccggtgcg gccgaccgcg cggatgccgg gaacccgggc gatctcacgc gcgatcgcct
    13021 cgctgcggtc gaccagctgg cggcggtcga gctcggcgat ctggaggatg ccctcgcgcc
    13081 gatagccggg gtcggcgctc agcgcgtgga gcgtctgggc atagaccacg acggtgcaga
    13141 tgatgaggcc gatcgagacg gcgaactggc cgaccaccag agccgcccgc agccggcccg
    13201 cgcccgccgc ctcggccgag gatttgttcg ccttcagcac ttcggcgggc tggaagcgcg
    13261 acaggtagaa ggcagggtag agaccgccga gcaggccgac ggcgagggtc agcagcagca
    13321 tggtcggcaa cattccctca gcgccgatat agtgggtctg cagctcggcg ccgagctggg
    13381 cggaaagcca gggcagcgac agctcgacga tggcgagcgc gaccagcatc gccagaccgg
    13441 cgatcagcac cgattcgacc aggaactgga gcatgagctg gcgccgggtc gcgcccagca
    13501 ccttgcggag cgccacctcg cgggcgcgct ggccggcgcg ggcggtggtg aggttgatga
    13561 aattgaccac cgccatagcg aggatcagga aagcgatggc gacgaaggtg acgatggtgc
    13621 ggcggtcgtt gccggggctc atggcgcctt cggtgacgtc gccgagatgg acgtcgcgga
    13681 tgttggccag gctccaatcg ccctcgtccc cgaaattggt gcgcccgccg tcgctagtgt
    13741 cgtcggggat gttgcgcttc tcccaggccg ggagctgcgc agcgatggcg gcgggatcgg
    13801 ttccggggcg aagcgcgagg tagttccagc cctggtacca gccccaggcg gtgaggaatt
    13861 cggggctttc ggcgaagtag ctgccgggat cgaagcgcgc gaccatggcg aggcgcatgt
    13921 gcgaattgcg cggcagatcg cgcatcacgc cggtaacgcg gtgatcgatc ctgttgccct
    13981 gcatgaccag agtcagcgtc tcgccgatgg catcggcgcc gccgaaatat ttgcgcgcct
    14041 cgctttcgct cagcaccacc gagcccggct cgcgcagcgc cgtggcgggg tcgccgtcgc
    14101 tgaacgggaa gtggaacatg tcgaagaagg ggccgtcgac cagcaccacg tcctcggtcg
    14161 gcagcgcctg gccgcgatgg atcaccaccg gcgcggccga aaaggcgtag gcgacgcgct
    14221 cgacctgcgg gaaatccttg gccagcgttt tgccggccac gtaggcggac atctgggtat
    14281 ccgtgctctc gccggtctcg ggatcgcgcc agtggctctg gacctggtag atattctcgg
    14341 cattggggag ccaggcgtcg tagcttcgct catgctgaac gtagagcagc agcagcatgc
    14401 acgcccccat gccggcggcg aggccgaaga tggtgatgaa ggcgtagctc ctgttcctca
    14461 ggagcgcccg caggccgacg atgagataat tgcgccacac gaaacgaatc tccccccgct
    14521 ctggcttagc acccggggga gtcgggaagg aatcgggatt ttacgcgagg cgccgtgctg
    14581 cggtttctac ctagggtctg cggtttatct cagggatcga cgccggtggc gatcgcctcc
    14641 agcttgcgca gccgctcgcg gacgtcggcg agctcgatgc gcgcgcccga accgccgccc
    14701 tcgccgagcc cgccatggcg agcggcgatc tcgtagcgct tgagctccag ccagccgcgc
    14761 caggcgcgca ggaaggcgaa ggcgatggtg ccggtgacgg cgagcgcggc gcccgcccag
    14821 acgaattcgt tcatgatcat cctattcgta acgcagggca tggatggggt tggcgcgggc
    14881 gaccttcagc gcgtggccgg cgatggtgcc gatcgcgatg gcgagcgcga ttaagccggc
    14941 gagcaggaac ggcgtggcac cgagcgcgat gcgggtgtcg aagctgttaa gccagtcgcg
    15001 catcacccac caggcgatcg gccaggcgag gaggttggcg acgatcaccg gcttggagaa
    15061 ttgccaggcg agcagccgga cgatgtcgcg gctgcgcgcg cctagcacct ttcggatgcc
    15121 gatctccttg gtgcgccgct cggcagcaaa ggcggcgagg ccgaacaggc cgaggcacgc
    15181 caccaccatg gccagcaagg cgaagccgcc gaaagtcttc gcccgcgcct cctcggcatc
    15241 gtaaagctcg gcgacgatgt cctcgctgaa ccgggcctcg aacggcacat cggcggcgag
    15301 ccgcttccac accgcctcga tccggtcgcg caccgcattg ggctcggcgc tgttgtagcg
    15361 gacgacgagg tgcgacagat aatcgccgga gagccggtac atgattgggt cgatcggctg
    15421 gcggaccgac cgaaagcgcg aatcctcgac cacgccgacg atggtcagcg gcaccaggcc
    15481 ggcttcgggc gcgttgaaga agatggcggt gcgaacggtg gcgccgaccg cgtcggccgg
    15541 attggcgaag ccgagccggc gcgcggccag ctcgttgatg acgacgttgc cgccgcgcgc
    15601 cgccatcgcc tgcttctgct cgagcggggc ggtgtcgggc acggaggtgt cgtcgagcgg
    15661 ccggttgcgg tcgagcagcc gaccggcgac gcgcttgatc cccatcactt cgaagaagcc
    15721 ggcgtcgatc ggataattgc cgatgctgac gccctcgtcg cggccgggga cgtagctgtt
    15781 gcggttcatg ttgctggggg tggacacgcc gatgctggtg cgcgcggcgg cggtgacgcc
    15841 ttcgacgcgc tcgacctcgc gcaccaaggt ctcgccgagc gccaccatct gcttgcgggc
    15901 gatgccggag atttgaagga cgccctcgcg cttgtagccg ggatcggcgg tgcgcgcgta
    15961 catggtctgg gaatagacga tcgcggtgca gatgatgagg ccgatcgaga ccgcgaactg
    16021 cgcgaccacc aaggcgctgc gcagatagcc ggcgccgggc gtctcggcgg ccgacttgtt
    16081 ggccttgagc acgcgcgcgg gctggaagcg ggagaggtag aaagcggggt agaggccgcc
    16141 gatcgcgccg accaccagca ccatcgccag gatcggcagc agcacgccct tttgtccgag
    16201 ataggtgagc ctgagatcgg catcgaggaa gcggccgagc gcgggcagca gcaattcgac
    16261 cagggccagc gcgaccagca tcgcgatcga cacgagcagg accgattccc cgaggaactg
    16321 cacaatcagc tggcggcggg tggcgccgac aaccttgcgc aggccgacct cgcgggcgcg
    16381 ctgggtggcg cgggcggtgg cgaggttggt gaaattgacg caggccatgc cgaggatcag
    16441 caaagccacc acggtgaagg tgaggatggt gcggcggtcg ttgccttcgc tcatcgcgcc
    16501 gccctgttgc tgcgcgaggt ggatgtcgcg gacgttgacg aggtgccaat cctggttctc
    16561 gccgacgttg atggtctcgc cgccgaaggt ctgggtcggt atgttgcgcc gctcccaggc
    16621 gggcatctcg gcgtggatgt cctcgagccg ggtgccgggg cggagcgaga catagttcca
    16681 gccgttggtg ttgccccatt cgctcgcata attgggctcg tcggcgaaga cgcgggcggg
    16741 atcgaagcgg acgatcatgt tggcgcgcag atgcgagttt ttcggaagat cgggcatcaa
    16801 cccggtgacg cggtgatcga catgctcgcc gcgcgagacg agcgtcagcg tccgcccgat
    16861 cgggtcggcg tcgccgaact gccggtgggc ctcgctctcg gtcagcacca ccgagttggc
    16921 ctgggtgagc gcccgggcgg ggtcgccctt cagcagcgga agctcgatca cgtcgagcag
    16981 gttgccgtcg gtgtataggg tgtcgtcgac agtgctgacc tcgccgccgc gataggcgac
    17041 ggcctggccc gcgaaggtgt aagcgagctt gtcgatctgc gggaaatcct tcttcatcgt
    17101 cgcgccgacc gggtagggcg acatctggaa gcccccgcgc tcgccggtgt ccgggtcggt
    17161 ataggtggag tggacctggt agacattctc ggcattgggc ag
//
LOCUS       DASVRK010000261         9421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_428317, whole genome shotgun sequence.
ACCESSION   DASVRK010000261 DASVRK010000000
VERSION     DASVRK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428317"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            539..748
                     /locus_tag="VF589_10415"
     CDS             539..748
                     /locus_tag="VF589_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258037.1"
                     /translation="MAEIERDDGDDGEGPLKAEALARDEWRKGETFLDEGDAPGHTLP
                     ESERDRARMKSPGGGGSAGPMAPPD"
     gene            complement(944..1132)
                     /locus_tag="VF589_10420"
     CDS             complement(944..1132)
                     /locus_tag="VF589_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEX8258038.1"
                     /translation="MPAKSQAQQKAAGAALSAKRGDTPKSKLKGASKSMAESMSETQL
                     EELAATKRKGKPEHKSDS"
     gene            complement(1194..1613)
                     /locus_tag="VF589_10425"
     CDS             complement(1194..1613)
                     /locus_tag="VF589_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258039.1"
                     /translation="MRMLAACTALAAGLGACTETSAAPDAAASAPPAAREIVEVEALP
                     QGCALTVRFGSYAMGIDGGAARRIEALLTTDPRVSAVSRHGRGREGEYALCAETRAAA
                     DGSALLADIRPLLPAKPRGPIEIKLADGTRLHAPPRR"
     gene            1735..2448
                     /locus_tag="VF589_10430"
     CDS             1735..2448
                     /locus_tag="VF589_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX8258040.1"
                     /translation="MDEIEWWEFASQAELAEQAAGDIGFVVESAIGAHGGARLALAGG
                     PKRDHIYAALVKQKLDWAKVTVLPTDDRLVGHDEEISMVRQLGVHFAPVGANIVPLVD
                     EAALDNYREAGRLADERLKMIDWPLDLVLLGMAADGHTASIFPGPDFDRAVNGPKERR
                     AIGVRPDPMPDFAAVERVTLTAPALASARAVMIAIAGQAKRELLERAIREGPLSSLPI
                     GRVLAAIEAPIDIFWSAEE"
     gene            complement(2501..3754)
                     /locus_tag="VF589_10435"
     CDS             complement(2501..3754)
                     /locus_tag="VF589_10435"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEX8258041.1"
                     /translation="MSLREINFDGIIGPSHNYSGLSLGNLASSSNKGNVSHPRAAALQ
                     GLDKMRHNLALGLAQGIFLPHKRPDADWMAELGTRVEQVPASLRPAAFSASAMWAANA
                     ATVSPPPDTADGRCHLTVANLRTMAHRSHEWPETLRQLRLAFANDEHFAVHAPVPATF
                     GDEGAANHMRLGASHNSPGVEVFVYGKRGGAFPARQHVEACAAIARYHRLDPDRTLFV
                     AQAEEAIAAGAFHNDVVAVANEFVLFAHEQAFEDRADVYTSLRRLLPEVEIVEVPASA
                     VSLEDAIKSYLFNAQLVTLPEGGMGLVLPEEARETPSVWAWLEQMVAGNGPIRKLFVV
                     DVRQSMANGGGPACLRLRVVTDPALVDRRFLVDPARLDAIGSVIAQHWPERIAPEDLL
                     SPELWRQIETARLALLEATGLTELA"
     gene            complement(3757..4776)
                     /locus_tag="VF589_10440"
     CDS             complement(3757..4776)
                     /locus_tag="VF589_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEX8258042.1"
                     /translation="MSFRIRPANGDDFQAIYEMAKLTGGGFTNLPPDRASLVDKLIRS
                     QTAFETDSEVQGDDLFLMVLENTDTGQIRGTCQIFGQVGTEWPFYSYRLNTLTQTSKA
                     LGKTFRAEMLTLCTDFEGSSEVGGLFLHPGERAGGLGLLLARSRYLFIRQHRKRFGDR
                     VLAELRGVIDEAGGSPFWDAIAGKFFGMDFREADEFNAMHGTQFIADLMPKTPVYTAM
                     LPDSARAVMGVPHPSGRAAMKMLEREGFHYDCYVDIFDGGPTMAARTDDIRTVRDARL
                     LPLAGISDDIDTEPMMLAAGRLGSFVACYGHVGLEPDGTAMIDARSAEMLGVGAGDTF
                     LAMGR"
     gene            complement(4773..5984)
                     /locus_tag="VF589_10445"
     CDS             complement(4773..5984)
                     /locus_tag="VF589_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX8258043.1"
                     /translation="MRGLSTIEAAAVEACAEAPMLDQVLRWSAINSGTRNLDGLAAVG
                     AMLAEEFGLLPGDPMLREPADVQAIDADGRLRDVEHGFNLHLKVRPEAPVQLLFTGHM
                     DTVYGADHPFQDMVWREAGVLNGPGVADMKGGLAVMLAALAAVERSPAAGRLGYEVVI
                     NSDEEVGSPGSAPLLAAAARGKRAALTYEPAALPDGTLAGARPGSGNFSIFVQGRSAH
                     AGRNPEEGRNALLAAADLALRLAAAKRPGLSVNPARIDGGGPNNVVPDRALLRVNMRP
                     ATAEDQALAQAALDAAIAAVAAEHEVAIHLHGGFARPPKPIDPAAERLFELVRRCGED
                     LGQSISWRASGGVCDGNNIAACGVPVVDTMGVRGGAIHSAEEYLIVDSLVERAQLSAL
                     TILRLALGENP"
     gene            6095..7387
                     /locus_tag="VF589_10450"
     CDS             6095..7387
                     /locus_tag="VF589_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8258044.1"
                     /translation="MAETDPQLPAAAPEPDAPERPEPRREAAAAPKDEAAEAPPAPAA
                     ADDPLLESIEIAYGRDRLLAALTLLAGAGFAIALPFALRAGAEFFLPVTAALVIAIAL
                     VPLLEWFERRRVPSGLAALICVLLFIIVANAAVAAIVLPATEWVRLLPERIGRIRETL
                     APLLDVYASFERFIDEVVTEFGSEPTGEPQTVAVETPNSMLDLIATSAPHAAIQMFFA
                     LLVIFFFLSGWTRMRKQTITTRTSFDGAMTTARVIQQVVDATSTYLGTITLVNVGMGL
                     FVSLILYLIDMPTPLMWGGIVAVLNYIPYLGPIASVLLLALGGLMAFADPWYALLPAV
                     SFAMIHLVEANIVTPSIVGRRLTINPLLILIALSFWAWVWGTTGALLAVPLLIILKTV
                     LDAAGKPDIAGFLFEEGTLTHTHYDELIEDHGGHRNKR"
     gene            7440..7516
                     /locus_tag="VF589_10455"
     tRNA            7440..7516
                     /locus_tag="VF589_10455"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7474..7476,aa:Asp,seq:gtc)
     gene            7718..>9421
                     /locus_tag="VF589_10460"
     CDS             7718..>9421
                     /locus_tag="VF589_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258045.1"
                     /translation="MATYEGTSGSDVIAGSADRDEINGYQGNDQLFGLDGDDELFGHD
                     GNDRLVGGAGRDFLNGGLRGDDVMEGGADDDRYSVETAGDVIIELAGEGTDTVDAYCD
                     FTLPDHVEKLSLRGDSTRGTGNSSANLISSFAADPERDHLLEGMAGDDRLFGSSEGFD
                     HLLGGEGDDILTSKGIDLLEGGVGNDTLVIGGLYGAELFGGEGNDILFAGRHGGLHGG
                     AGDDIYEFDSNDSSRVVEHAGEGRDVVNASRNYALGANLEDLVLAGDGRRGVGNALDN
                     VITGSGGFDGLVGMEGNDTIRGGLGNDVISGGGDSDLLEGEDGDDQMTGGAGFDRLRG
                     GAGMDQLSGGSGEDELHGGADADDLEGNSQDDLLAGDEGDDRLAGGGGTDRLEGGDGD
                     DLLDGGDGRDSLHGGEGFDIASFASQFNGVRVRLEPAGAGIDDLISIEGVIGTRSADS
                     LRGDANDNMLNGGEGDDSLTGGGGNDLIIGGDGRDSANYNFSVGGVTVRLDTEDAQST
                     GGAGTDRLVGIEDLIGSTSGDDLTGHGGANRIDGRGGDDSLSGLQGDDELIGQGGHDR
                     LS"
BASE COUNT         1473 a         3004 c         3431 g         1513 t
ORIGIN      
        1 cctcccccac ccccggacct aaaaagtaga ctgaggtata cgctaactgc cagccgagat
       61 ggggtaggtc cgctaaccct atctcggccg gtcgacgatg taggccttgg cttcaactgc
      121 ccttcagcgc cagagctatc cgggcacccg ctcgtcccaa tggatgtggt atccgctgac
      181 cggcggctgc accttgtcaa cgaaggtctt agtgagcttc gccatggcct gctcctcgtg
      241 ctgatgctag caccatacca gcaaggcagg aacagctacg acaatctgca acaaactagc
      301 aacggcataa gtgcctgatt ttataggctg aaacgtgcta gcacaaacta gaacagtcta
      361 tctgagtcag cgagggacca gactacgaat ctgggggtcg gaggttcgaa tccttccggg
      421 cgcgccattc ctttttgctt atcgataatc gcttcttgcc agggcgttgg cgggccgtgg
      481 ccccgcgcgg aacggaggcg caggggcgga cgttcggggg ctcgaagagg agccggtgat
      541 ggcagagatt gaacgcgacg atggcgacga cggcgagggt ccgctgaagg cggaggcgct
      601 ggcgcgggac gagtggcgga agggcgagac cttcctcgac gagggcgacg cacccggcca
      661 caccttgccc gaaagcgagc gcgaccgcgc ccggatgaag tcgccgggcg gcggcggttc
      721 ggccgggccg atggcgccgc cggattgagc ggcgggtgcc ggcgtgcgag ccggtgaggt
      781 gaaggcgacc gctccggtga agtcacttcg gcgtgaagcg acatcgtcat tcccgcgaaa
      841 gcgggaatct ccccttcctt cagacggttg gaagaagaag tgagatccca gctttcgctg
      901 ggatgacggg gagggcgttc tactcatctt tgcgccagcc ccgtcagctg tcgctcttat
      961 gctcgggttt gcccttgcgc ttggtcgcgg cgagctcttc gagctgcgtt tcgctcatcg
     1021 attccgccat cgacttggac gcgcccttga gcttgctctt gggggtgtcg ccgcgcttgg
     1081 ccgacaatgc ggcgccggcg gctttctgct gggcctgcga tttggccggc atgatcgtgt
     1141 ctcccgttgc gatgccggag gaacccgcgc tctccgggtg cggttccggc cggtcagcgg
     1201 cggggcggcg cgtggaggcg ggtgccgtcg gcgagcttga tctcgatcgg gccgcgcggc
     1261 ttggcgggga gcaggggccg gatgtcggcg agcaaagcgg agccgtcggc cgcggcgcgg
     1321 gtctcggcgc acaacgcata ttcgccctcg cggccgcggc cgtggcggct gaccgcggaa
     1381 acacgcgggt cggtggtgag gagcgcctcg atgcgccggg cggcgccgcc gtcgatcccc
     1441 atcgcatagc ttccgaaccg caccgtcagc gcgcagccct gcggcagcgc ctcgacctcg
     1501 acgatctccc gcgccgcggg cggggctgag gccgcggcgt cgggggcggc actcgtctcg
     1561 gtgcaggcgc cgaggcccgc ggccagggcc gtacaggcgg ccagcatcct catgtccgcg
     1621 tctccctcgt tcgtcccatc catgccaacc gaacggaccg ggcgccaatt ggatgccgct
     1681 ttcctttgcc cgccgccgcc gagccgctaa gggagcccgg cagaaggaga cgctatggac
     1741 gagatcgaat ggtgggagtt cgcttcccag gccgagctgg ccgagcaggc ggcgggcgat
     1801 atcggcttcg tcgtcgaaag cgcgatcggg gcgcatggcg gcgcgcggct ggcgctcgct
     1861 ggcgggccga agcgcgacca tatctatgcg gcgctggtca agcagaagct cgactgggcc
     1921 aaggtgaccg tgcttcccac cgacgaccgg ctggtggggc atgacgagga aatcagcatg
     1981 gtgcgccagc tcggcgtcca tttcgcaccc gtcggggcca atatcgtgcc gctggtcgac
     2041 gaggcggcgc tcgacaatta ccgcgaagcg ggccggctcg ccgacgagcg gctgaagatg
     2101 atcgactggc cgctggacct ggtgctgctc ggcatggccg cggacggcca taccgcctcg
     2161 atcttccccg gcccggattt cgaccgggcg gtgaacggcc ccaaggagcg gcgcgcgatc
     2221 ggcgtccggc ccgatccgat gcccgatttc gccgcggtcg agcgggtcac gctgaccgcg
     2281 ccggcgctgg cctcggcgcg cgcggtgatg atcgcgatcg ccggccaggc caagcgcgag
     2341 ctgctcgagc gcgcgatccg cgaagggccg ctgtcgtcgc tgccgatcgg ccgcgtgctg
     2401 gccgcgatcg aggctccgat cgacatcttc tggagcgccg aggaatagcg gcaggggaag
     2461 aagggagatg ccggatcaag tccggcatga cggcggaggc ttaggcgagt tccgtcagcc
     2521 ccgtggcttc cagcaaggcg aggcgggcgg tctcgatctg gcgccatagc tcggggctga
     2581 gaaggtcctc gggggctatg cgttcgggcc agtgctgggc gatcacgctc ccgatcgcgt
     2641 cgagccgggc cggatcgacg aggaagcggc ggtcgaccaa agcgggatcg gtgacgacgc
     2701 gcaggcgcag gcaggcgggg ccgccgccat tggccatcga ctggcggacg tcgaccacga
     2761 acagcttccg gatcgggccg ttgccggcca ccatctgctc gagccaggcc cagaccgagg
     2821 gcgtctcgcg cgcctcctcg ggcaggacca ggcccatgcc gccctcggga agggtgacga
     2881 gctgggcgtt gaacaaatag ctcttgatcg catcttcgag gctgaccgcg ctcgccggaa
     2941 cctcgacgat ctcgacctcg ggcagcaggc ggcgcagcga agtgtagacg tcggcgcggt
     3001 cctcgaaggc ctgctcgtgc gcgaacagga cgaattcgtt ggcgacggcg acgacgtcgt
     3061 tgtggaaggc gccggcggcg atcgcctcct cggcctgggc gacgaacaag gtgcggtcgg
     3121 ggtcgagccg gtgatagcgg gcgatggcgg cgcaggcctc gacatgctgg cgcgccggga
     3181 aggcgccgcc gcgcttgccg tagacgaaga cttcgacgcc gggcgaattg tgcgaggcgc
     3241 cgagccgcat atggttggcg gcgccctcgt cgccgaaggt ggccggcacc ggcgcgtgga
     3301 ccgcgaaatg ctcgtcgttg gcaaatgcga ggcggagctg gcgcagggtc tcgggccatt
     3361 cgtggctgcg atgcgccatg gtgcgaaggt tggcgacggt gaggtggcag cgcccgtcgg
     3421 cggtgtcggg cgggggcgac accgtggccg cattggcggc ccacatggcg gaggcggaga
     3481 aagcggccgg gcgcaggctc gccggcactt gctcgacgcg ggtgccgagc tcggccatcc
     3541 agtcggcatc ggggcgcttg tgggggagga agatgccctg ggcgaggccg agcgcgagat
     3601 tgtggcgcat cttgtcgaga ccctggagcg cggccgcgcg cggatgcgag acattgccct
     3661 tgttgctcga cgaggcgagg ttgccgaggc tgaggcccga ataattgtgg ctcgggccga
     3721 tgatgccgtc gaaattgatt tcccggaggc tcatcgtcag cgtcccatgg ccaggaaggt
     3781 gtcgccggcg ccgacgccga gcatttccgc gctcctcgca tcgatcatcg cggtgccgtc
     3841 cggctccagc ccgacatggc cgtagcaggc gacgaagctg ccgagccgtc ccgccgccag
     3901 catcatcggc tcggtgtcga tgtcgtcgct gatcccggcc agcggcagca ggcgggcatc
     3961 gcggacggtg cggatgtcgt cggtgcgggc ggccatggtc ggcccgccat cgaaaatgtc
     4021 gacatagcaa tcataatgga agccctcgcg ctccagcatc ttcatcgcgg cgcggcccga
     4081 cgggtggggg acgcccatca ccgcgcgcgc gctgtccggc agcatcgcgg tgtagaccgg
     4141 ggtcttgggc atcagatcgg cgatgaactg ggtgccgtgc atggcgttga attcgtcggc
     4201 ctcgcgaaaa tccatgccga agaacttgcc ggcgatggcg tcccagaaag gcgagccgcc
     4261 agcctcgtcg atgacgccgc gcagctcggc cagcacgcgg tcgccgaagc gcttgcgatg
     4321 ctgccggatg aacaggtagc ggctgcgcgc gagcagcagg ccgaggccgc cggcgcgctc
     4381 gccggggtgg aggaacaggc cgccgacctc ggacgaccct tcgaaatcgg tgcacagcgt
     4441 cagcatttcg gcgcggaagg tcttgccgag cgccttggaa gtctgcgtga gggtgttgag
     4501 gcggtaggag tagaagggcc attcggtgcc gacctggccg aatatctggc aggtgccgcg
     4561 gatctggccg gtgtcggtgt tctcgagcac catcaggaac aggtcgtcgc cctggacttc
     4621 gctgtcggtc tcgaacgcgg tctgcgagcg gatcagcttg tcgaccagag aggcgcggtc
     4681 gggcggcagg ttggtgaagc cgccgccggt gagcttggcc atttcgtaga tcgcctggaa
     4741 gtcgtcgcca ttggcggggc ggatgcggaa gctcacggat tttcccccag ggcgaggcgc
     4801 aggatggtga gcgcggaaag ctgcgcgcgc tcgaccaggc tgtcgacgat cagatattcc
     4861 tcggcgctgt ggatggcgcc gccgcgcacg cccatcgtgt cgaccacggg cacgccgcag
     4921 gcggcgatat tgttgccgtc gcagacgccg ccggaggcgc gccagcttat cgactggccg
     4981 agatcctcgc cgcagcggcg gacgagctcg aacagccgct cggcggcggg gtcgatcggc
     5041 ttgggcgggc gggcgaagcc gccatgaagg tggatcgcga cctcgtgttc ggcggccacc
     5101 gcggcgatgg cggcgtcgag cgcggcctgg gccagcgcct ggtcctcggc ggtggcgggg
     5161 cgcatgttga cgcggagcaa tgcgcggtcg gggacgacat tgttgggccc gccgccgtcg
     5221 atccgggccg ggttcaccga caggcccggc cgcttggcgg cggcgaggcg caacgcgagg
     5281 tcggccgccg ccagcaaggc gttgcgcccc tcctcgggat tgcggccggc atgggcgctg
     5341 cggccctgga cgaagatcga gaaattgccg ctgccggggc gggcgccggc cagcgtcccg
     5401 tcgggcaaag cggcaggctc gtaggtgagg gcggcgcgct tgccgcgcgc ggcggcggcg
     5461 agcaacgggg cggagccggg cgagcccact tcctcgtcgc tgttgatcac cacttcatag
     5521 ccgagccgcc cggcggcggg gctgcgctcg accgcggcca gcgccgccag catcacggcg
     5581 aggccgccct tcatgtcggc gacgccaggg ccgttgagca cgcccgcctc gcgccagacc
     5641 atgtcctgga acggatggtc ggcgccgtag acggtgtcca tgtggccggt gaacagcaat
     5701 tgcaccggcg cttccggcct gaccttcaga tggagattga agccgtgctc cacgtcgcgc
     5761 agcctgccgt cggcgtcgat ggcctggacg tcggcgggct cgcgcagcat tggatcgccg
     5821 ggcaggaggc cgaactcctc ggccagcatc gcgccgacag cggcgaggcc gtcgaggttg
     5881 cgcgtgccgc tgttgatcgc ggaccagcgc aagacctggt cgagcatcgg cgcttcggcg
     5941 caggcttcga ccgcggccgc ctcgatggtg gaaagccccc tcatggaggg cgtctctaat
     6001 ccgattccgc ggccaaagcc aaccggggag aggaggcgcg gccgcgacga catgtctggc
     6061 gttcagcgcc atatcggggc atgttcgcag cgcgatggca gagaccgacc cacagcttcc
     6121 cgcggccgca ccggagccgg acgctccgga gcggcctgag ccccggcgcg aggcggccgc
     6181 cgcgccgaag gacgaagcgg ccgaggcgcc gccggccccg gccgctgccg acgatccgct
     6241 gctcgaaagc atcgagatcg cttatggccg cgaccggctg ctcgccgctt tgaccttgct
     6301 ggcgggggcg gggttcgcga tcgcgcttcc gttcgcgctt cgagccggcg cggaattctt
     6361 cctgccggtg acggccgcgc tcgtcatcgc cattgctttg gtgccgctgc tcgaatggtt
     6421 cgagcggcgc cgcgtcccat ccgggctggc ggcgctgatc tgcgttttgc tgttcatcat
     6481 cgtcgccaat gccgccgtcg ccgcgatcgt gcttccggcg accgaatggg taaggctgct
     6541 gcccgagcgg atcggccgga tccgcgaaac gctggcgccc ctgctcgacg tctatgcgag
     6601 cttcgagcgc ttcatcgacg aggtggtgac cgaattcggc agcgagccga ccggcgagcc
     6661 gcagacggtt gcggtcgaga cgcccaattc gatgctcgac ctcatcgcca cttccgcccc
     6721 gcacgcggcg atccagatgt tcttcgcctt gctggtgatc ttcttcttcc tgtccggctg
     6781 gacccggatg cgcaagcaga cgatcaccac ccggaccagc ttcgacggcg cgatgacgac
     6841 cgcgcgggtg atccagcagg tggtcgacgc cacctccacc tatctcggca cgatcacgct
     6901 cgtgaacgtc ggcatggggc tgttcgtctc gctgatcctc tacctcatcg acatgccgac
     6961 gccgttgatg tggggcggca tcgtcgcggt gctcaactac atcccctatc tcgggccgat
     7021 cgcctccgtg ctgctgctcg cgctcggcgg gctgatggcg ttcgccgacc cctggtatgc
     7081 tttgctgccg gcggtgtcgt tcgcgatgat ccacctggtc gaggccaata tcgtcacgcc
     7141 ctcgatcgtc ggccgccgcc tcaccatcaa tccgctgctg atcctgatcg ccttgagctt
     7201 ctgggcgtgg gtgtggggga cgacaggggc gctgctggcg gtgccgctgc tgatcatttt
     7261 gaagaccgtg ctcgacgccg ccggcaagcc tgacattgcc ggcttcctgt tcgaggaagg
     7321 cacgctcacc catacccatt atgacgagct gatcgaggac catggcgggc accgcaataa
     7381 gcgctagcct tgcttgacag cttagggggg gtcgcttagt cggcgccgca cgcatccacg
     7441 cgggtgtagc tcagttggtt agagcgccgg cctgtcacgc cggaggtcgc gggttcgagc
     7501 cccgtcactc gcgccattcc cgaactcccg aaccgatcgc ctcgccggcg cgcgctgctt
     7561 cgtggccgcc gcgcccggta gggtgccgaa gagcgttccc gcccggatcg ttaccgggcg
     7621 cgcgtcttag cttcgcgagc catccttttc atctgcatgg cttcttcggc cgaaatagct
     7681 tatgagcaac agcttctgta cgcggtgggg acgaatcatg gcgacatatg aaggcacttc
     7741 cggttcggac gtcatcgcgg ggtcggcgga tagggatgag atcaacggct atcaggggaa
     7801 cgaccagctg ttcggcctcg acggcgatga tgaacttttc ggccatgacg gcaacgatcg
     7861 gctggtcggc ggggcgggcc gcgatttcct gaacggcggg ctgcgcggcg acgatgtgat
     7921 ggaagggggc gccgacgacg accgctattc cgtcgagacc gccggcgacg tgatcatcga
     7981 gctcgcgggc gagggcacgg acacggtcga cgcctattgc gatttcactc tccccgatca
     8041 tgtcgagaaa ctcagtctgc gcggcgacag cacccgcggc accggcaaca gctccgccaa
     8101 tttaatttct tcgttcgccg ccgatcctga gcgcgaccac cttcttgaag gaatggcggg
     8161 cgacgatcgt cttttcggat cgtccgaggg gtttgatcat ctgctcggag gcgagggcga
     8221 cgacatcctg acctcgaaag ggatcgacct tctcgaaggc ggcgtaggca acgacacgtt
     8281 ggtgatcggg gggctgtacg gggcggagct cttcggaggg gaaggaaacg atatcctttt
     8341 cgccggacgc cacggcgggc tgcacggcgg cgccggcgac gatatctacg aattcgactc
     8401 taacgattcg tccagggtcg tcgaacatgc cggtgagggg agggacgtgg tcaatgcgtc
     8461 ccgcaattac gccctcggcg ccaatctgga ggatctggtg ctcgcggggg acgggaggcg
     8521 gggcgtcggc aacgcgctgg acaatgtcat caccggatcg ggcggcttcg acgggcttgt
     8581 cgggatggag ggcaacgaca cgattcgagg cgggttgggc aacgacgtca tcagcggcgg
     8641 cggtgactct gatttgctcg agggcgagga cggcgacgac cagatgaccg gcggcgccgg
     8701 cttcgaccgg ctccggggcg gcgcgggcat ggaccagctc agcggaggct ccggcgagga
     8761 cgagcttcac ggcggcgccg atgccgacga tctggaaggc aatagccagg acgacctcct
     8821 ggccggcgat gagggcgacg accgcctcgc cggcgggggg ggcaccgacc ggctggaagg
     8881 cggcgatggg gacgacctgc tggacggagg cgacggccgc gacagcctcc acggaggcga
     8941 gggtttcgac atcgccagct tcgcgagcca gttcaatggg gtaagagtga ggctggagcc
     9001 ggccggcgcg ggcatcgacg acctgatcag catcgaagga gtcataggaa cccgctccgc
     9061 cgactcgctc cgcggcgacg ccaacgacaa catgctgaac ggaggcgaag gggacgactc
     9121 gctgacgggc ggcggcggca acgacctcat catcggcggc gacggccggg acagcgccaa
     9181 ttataacttc tccgtcggcg gggtgacggt gaggctcgac accgaagacg cgcagtcgac
     9241 cggcggcgcc gggaccgatc gcctggtcgg gatcgaggat ctgatcggat cgacttccgg
     9301 cgacgatctg acgggccatg gcggggctaa ccggatcgac ggccgcggcg gcgacgattc
     9361 gctcagcggg ctgcagggcg atgacgagct catcggccag ggcgggcacg accggctgtc
     9421 c
//
LOCUS       DASVRK010000262        11154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_363997, whole genome shotgun sequence.
ACCESSION   DASVRK010000262 DASVRK010000000
VERSION     DASVRK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363997"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..603)
                     /locus_tag="VF589_10465"
     CDS             complement(88..603)
                     /locus_tag="VF589_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258046.1"
                     /translation="METASIIATHTPVYVWLVLALLLFLGVRRLKPRRTHLALAAPAP
                     LGFLTWSVLAAGLLFFGGDKETSVVAWGVAFLTGALSGPIRTVPSPKHMHGWVFEYSA
                     TELPLTLYMLLWATRYGLGIWAGFVPAMADALGLAGLALSALTAGRTLADFIPPLATA
                     LRVRKAGIEAI"
     gene            complement(760..1230)
                     /locus_tag="VF589_10470"
     CDS             complement(760..1230)
                     /locus_tag="VF589_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258047.1"
                     /translation="MSTSADEFDYASTTVPDAALFVAADEPLLVFPSATAAEQYLEAI
                     DVENGVYPVAFGPRGEPYRVGTNGDQVVIERTGEPERPGELRSLLLRYLEGSRRLADP
                     ATSLEALVAEVWKTESDFWQEHDLYGERFGSRIPLWGCLASILVLATALYLILG"
     gene            1428..1718
                     /locus_tag="VF589_10475"
     CDS             1428..1718
                     /locus_tag="VF589_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX8258048.1"
                     /translation="MTAYVHVNIRIKDPARQAALVPRFQAALAAAGGRILHFGPVART
                     LEGDVAPLPLAGIFEFPTLADAMAFYQSPEYAPIKVERDAAQEARMFVVDTG"
     gene            1827..2288
                     /locus_tag="VF589_10480"
     CDS             1827..2288
                     /locus_tag="VF589_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258049.1"
                     /translation="MNGHKLIWAAVLPLAACQSSAMTATGGSTARIGQTALIDTIQVS
                     PTRLIEDSRCPALVRCIWAGRLRIEATIRYRGGSEELHREMILGEAISLPEGRLTLAA
                     AEPAPVAGKAVPASAYRFTFTLIPSAASGSAGVGSGRCEGLCLYKGDGADH"
     gene            complement(2653..3582)
                     /locus_tag="VF589_10485"
     CDS             complement(2653..3582)
                     /locus_tag="VF589_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8258050.1"
                     /translation="MQPFDLAMRRAEALLRAGNSVGAIEPLRDALTAAPQEPYPHLLL
                     ARALRAQNRLAGARYEAGRAVELAPLWAPAHVELAQALMMQQHGKPALASADKAVELD
                     PGDAYAHLVRAKLLRMARRRAEAQASLQAALQLEPDAPDIIAEQGYAALEVGDIAAVE
                     TAGREILSLNPEHADGLILTGQGRLARGDTEEALRLALAALSRSPTDLDALFLLAAVK
                     MRRNPIGGLWWRWNRMLVKLGEARAIFLVVGIWLVYRWLVLASEDFGFPPDTGSVLTI
                     VYLGFVLYTLSASVIVGRMIAREVERVRLDPSF"
     gene            complement(3587..3967)
                     /locus_tag="VF589_10490"
     CDS             complement(3587..3967)
                     /locus_tag="VF589_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258051.1"
                     /translation="MTDSLRYRLIDEPRPGPLGRIALPPLLVFIVAQIFLPWGALLIA
                     ANAVALNGPHRNREIALSAGPIVTYFAALAALDAAVRSGLLRVNQAHYFFVAALALGL
                     LFAAAAYVSQHRTAALRRYLREQG"
     gene            complement(3964..5328)
                     /locus_tag="VF589_10495"
     CDS             complement(3964..5328)
                     /locus_tag="VF589_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8258052.1"
                     /translation="MTINPAIEALLAAFRAGGDARMLSAALDLAAGDADQRAVIAAAG
                     EAPDAALDLGLRERLASAALDLGDALDAIRLCGDERTLLPIRVDALLVAGRVAEAESV
                     YRAAVAADPVLEDPALDERLAEAKAAAEPGMVSNVIGFAQAARGLRGAERSDREEEAG
                     ARALFAETTGIGFADVGGLDEVKKQITRRIVTPFRKPSLFAKYRRKAGGGVLLFGPPG
                     CGKTLLARATAGECDARFVNVPVVDVVDKYIGEAERKLAAIFADARRDTPTVLFFDEL
                     EALAGSRSGNANQSQISLVSTFLAEMDGFAHNNEGVLILAATNMPWGVDSAFRRPGRF
                     DRVQFVPPPDRGARAAILRLQLSGRPLGDDCDVEAIAAKTAGYSGADLENLVNTAVDL
                     AIEDALASGVESPVAQRHLVEALAEVRPTTLEWLTTARNYAKYSNAGGQYDDVAAFLK
                     ANGL"
     gene            complement(5448..6884)
                     /gene="nuoN"
                     /locus_tag="VF589_10500"
     CDS             complement(5448..6884)
                     /gene="nuoN"
                     /locus_tag="VF589_10500"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX8258053.1"
                     /translation="MGNSLLLTLPEIILSLSSLALLMVAAFVGDKASRLVTWLAVAAF
                     LLAALFIPGIRDAGAVGFDGLYVADSFAAFSKILIYIAAAVALVAAQGWFARDGVFRA
                     EYPVLILLSGVGMGMMVSAGDLLTLYVGLELQSLSAYVLASFQRRDTRSAEAGLKYFV
                     LGALASGILLYGVSLLYGFTGSTLFGDISLAMGRDGVSTGELFGLVFVMAGIAFKVSA
                     VPFHMWTPDVYEGAPTPVTAFFASAPKVAAMALLTRVAIEGMGPATFEWRQIVIFMAL
                     ASTILGGVAAIGQPNIKRLLAYSSINNIGFALIGLAAATREGVASVLFYMAVYVVMTL
                     GTFLVVLRMRDAEGRPVETIVSLSGLSRTRPGLAAAMAAFMFSLAGIPPLFGFWPKFL
                     VFNAAVAADLTWLAAVGIATSVIGAYYYLKIIKIMYFDDPAPAYERSPDRLGGALIVA
                     AALFVSPLGYFSIPLLESAAHNAAGALF"
     gene            complement(7050..8615)
                     /locus_tag="VF589_10505"
     CDS             complement(7050..8615)
                     /locus_tag="VF589_10505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX8258054.1"
                     /translation="MNALGFPILSLMLAVPLIAALICLKLGDSSARWLALWTTALELA
                     LGIALWFSYDIGGPQWQFQEYQPVFGRFAWALGIDGIALMLILLTVFLMPFCIAASWS
                     SIDRRIPEYMASFLLMEALMIGVFVAQDIFLFYIMFEAGLIPMFLIIGIWGGAQRIYA
                     SYKFFLYTLLGSVLMLVAMLWMVNHAGGETFIPRLMATDFPAAAQTWLFLAFFASFAV
                     KMPMWPVHTWLPDAHVQAPTAGSVILAGVLLKMGGYGFIRFSLPMFPEASAQLFWLVM
                     GLSMVAIVYTSLVALVQQDMKKLIAYSSVAHMAFVTIGLFAFNRQGIEGGLMVMLSHG
                     LVSGALFLCVGVVYDRLHTRDIDRYGGLSDNMPAYAVLLLLFTMASIGLPGTAGFVGE
                     FLSLIGAYRANTLVAVVATTGIILGAAYMLYLYWRVAYGTQRNPDAAAMPDLTRLEFF
                     ILAPIAAVVLWMGVYPESFLAPMRNDVGTLLARIERAAPPSDSAPAMGRAARPTGDSG
                     SHAAPAAAEAGAH"
     gene            complement(8827..10923)
                     /gene="nuoL"
                     /locus_tag="VF589_10510"
     CDS             complement(8827..10923)
                     /gene="nuoL"
                     /locus_tag="VF589_10510"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX8258055.1"
                     /translation="MIQLIVFLPLLAAIVAGLGNRALGNTAAKLITTGALFASCALSW
                     PIFLGFMAGNYQGGWVEPVLAWIHSGDLRVDWSLRVDALTAVMLVVVTTVSALVHLYS
                     WGYMAEDDSQPRFFAYLSLFTFAMLMLVTADNLVQMFFGWEGVGLASYLLIGFWYHKP
                     SANAAALKAFVVNRVGDFGFSLGIFGTFLVFGTVSIPDILAAAPGMAGSTIGFLGYRV
                     DTMTLLCILLFIGAMGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVCRLS
                     PMFETSQTALGVVTFIGAATALFAATVGTVQNDIKRVIAYSTCSQLGYMFFAAGVGAY
                     SAAMFHLFTHAFFKALLFLGAGSVIHAMHHEQDMRFYGGLRKSIPLTFSAMLAGTLAI
                     TGVGIIQVGGFAGFGFAGFYSKDAIIESAFASGTTIGGVAFTVGIFAALLTSFYSWRL
                     IFLTFFGKPRWAASGHIQHALHDADHGRSHDHEADPHPEDEHAGHDTRPGEASARQVP
                     AGTGGYHPHESPWTMLVPLGVLSVGAIFAGLLFHGPFIDAHEGVEFWRGSLAFDAHLM
                     EEIHHVPLWVKLLPGLVMIVGFLIALQAYVRHTSFPERFVEQFRVLHGFLLNKWYFDE
                     IYDLVFIRPSLWFGRLFWKRGDEGTIDRFGPNGAAALVSGGNKLTARLQSGYLYTYAL
                     VMLLGLAAAATWAMAR"
     gene            complement(11037..>11154)
                     /locus_tag="VF589_10515"
     CDS             complement(11037..>11154)
                     /locus_tag="VF589_10515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEX8258056.1"
                     /translation="MFVLTVAAGEAAIGLAILVIYFRGRGTIAVDDINQMKG"
BASE COUNT         2134 a         3495 c         3947 g         1578 t
ORIGIN      
        1 aagcggcgac ccggggctgg gcccctgcct tcgcaggggc acgatgccct tgcgcagtcg
       61 tgccgatcgg acattcgcct gcacctgtta tattgcctcg atccctgctt tccggacgcg
      121 aagagcagtg gcgagtggcg ggataaagtc agccagggtt cttcccgctg tgagggcgga
      181 cagcgcgagc ccggccagtc caagggcgtc tgccatcgca ggcacaaagc cagcccatat
      241 tccaagcccg tatcgcgtcg cccaaaggag catgtagagc gtgagcggca gttccgtcgc
      301 cgaatactcg aaaacccatc catgcatatg tttcgggctc gggaccgtcc gaataggccc
      361 gctgagagcg ccagttaaga aggcgactcc ccaagcgaca accgaagttt ccttgtctcc
      421 gccgaagaac agcaagcctg cggccaggac gctccacgtg aggaatccga ggggggcggg
      481 cgcggccagg gcaaggtgcg tgcgcctcgg tttcaatcga cgaacaccga ggaagagcag
      541 gagcgccaaa accagccaga catagaccgg tgtgtgcgtt gcgatgatac tcgcggtttc
      601 cattggcacc gatccttgca cctgtgatgt gaggccgcaa cggctctgaa aaaatccatc
      661 ggctgaatgt ccgctaaggg tcgacacgta tcgcctcgtt tggaagccgg ccgccggcag
      721 ccgcgaacgt ccgcaatgcg tggaaagcgg acatcaaggt caaccaagga tcaggtagag
      781 agccgtcgcc aagacgagaa tggaggccaa gcagccccac aagggtatgc gagacccgaa
      841 ccgttcaccg taaagatcat gctcctgcca aaagtcgctc tcggtcttcc aaacctccgc
      901 gaccaacgcc tcgagtgatg tggccgggtc ggccagacga cggctgcctt ccagatagcg
      961 caggaggaga gatctaagct ctccgggccg ctcgggctca ccggtccgct ctatgactac
     1021 ctgatctcca ttggtcccga cacggtaggg ctcgcccctc gggccaaaag caaccggata
     1081 gacgccattc tccacgtcta tggcttcaag atactgctct gctgccgtgg ccgaggggaa
     1141 gaccagcaac ggctcgtcgg cggccacgaa cagtgcggca tctggaaccg tggtggaggc
     1201 atagtcgaac tcgtctgcgc tggtgctcac ctcctgattg tttcaccgga agctaatgtc
     1261 cgcaatgggt cgaaagcgga ccttcagcgc tatctgtcct ggagttgacc gccttactca
     1321 tgcctcggct aatggctgga tccccgcgaa cggccgaaac cgtggaaagc agaaacaggc
     1381 gcggctagtg aacgccagcg agcaagcagc aaattcgggg agccgccatg acggcctatg
     1441 tccacgtcaa catccgtatc aaggatccgg cgcggcaggc cgcattggtg ccgcgttttc
     1501 aggcggcgct agcggcggca ggaggccgta tcctccactt cgggccggtg gcccggacgc
     1561 tggaggggga cgtggcgcca ttgcctctgg cgggcatctt cgaatttccg acgctggccg
     1621 acgcgatggc cttttaccag tcgccggaat atgcgccgat caaggtggag cgcgacgcgg
     1681 cccaggaagc gcgcatgttc gtcgtcgata cggggtgaag gcttcgaggc tgcgatggcg
     1741 ttccgtcagg ctgcaacggc ccggaaccgg aggtcggcaa cggcccgcga ttgccacatg
     1801 tccgtgcgcg tgcaagcgtg cgccgcatga acgggcataa gctgatatgg gcggcggtgc
     1861 ttccgctcgc cgcatgccaa tccagtgcca tgacggctac cggggggtcg acggcgagga
     1921 tcgggcaaac cgcgctgatc gacactattc aggtcagccc caccagacta atcgaggaca
     1981 gccgttgtcc ggccctggtg cgctgcatct gggccgggcg cctgcgtatc gaagcaacca
     2041 ttcgatatcg cggcggcagc gaggaactgc accgggagat gatcctgggc gaagcaatat
     2101 cgttaccgga agggaggctg acgctggccg cggccgagcc tgcccccgtg gcaggcaagg
     2161 ccgtgccggc gagcgcctac cgctttacgt tcacgctgat tccgtcggcg gcatccggtt
     2221 cggctggggt gggaagtggg cgttgcgaag ggctgtgcct ttacaaggga gacggagccg
     2281 accactgacc cggaatgcca gtcgtcgagt tcgtaaacca caatcgcgag acgccgctgg
     2341 tgctgcagat ccagccatgg gaagcatttc ggatcggcct cggtggacgc tgaagagctg
     2401 cgccgaaact ccgcgcggcc gtttggagac gcatcctcct gagctcccat ctgggggacg
     2461 agcggacgcc ccgcccgagc gcccgcaatg cgggggcgtt cggcatcggc ggtgctcgac
     2521 cggcgcgtgc cttttgctcc atttcctggc ggccgggcca aggccgcgcg cgtgggagga
     2581 gcggatgacg aagaggccga cacacggtcc cggactcgcg cgatgccccc cctcaccacc
     2641 gcgactacgc cctcaaaagg atgggtcgag gcggacccgc tccacttccc tcgcgatcat
     2701 tcggccgacg atcacgcttg cggacagggt gtagaggacg aagccgagat agacgattgt
     2761 gaggacgctc ccggtgtcgg gcgggaagcc gaaatcctcg ctggccagca ccagccagcg
     2821 atagaccagc cagataccga cgaccaggaa gatggcgcgc gcttcgccga gcttcaccag
     2881 catccggttc cagcgccacc acaggccgcc gatgggattg cgccgcatct tcaccgcggc
     2941 gagcaggaac aaagcgtcga gatcggtcgg cgagcgggag agcgcggcca gcgccaggcg
     3001 cagcgcttcc tccgtatcgc cgcgggcgag ccggccctga ccggtcagga tcagcccgtc
     3061 ggcatgctcc gggttgagcg acaatatctc gcggccggcg gtttcgaccg ccgcgatgtc
     3121 gcccacctcc agcgcggcat agccttgttc ggcgatgatg tcgggcgcgt ccggctccag
     3181 ctggagcgcg gcttgcaggc tcgcttgggc ctcggcgcgc cggcgcgcca tccgcagcaa
     3241 tttggcgcga accaggtgcg cgtaagcatc gccgggatcg agctcgaccg ccttgtcggc
     3301 cgatgccagc gccggcttgc cgtgctgctg catcatcagc gcttgcgcca gctcgacatg
     3361 cgcgggggcc cataagggcg ccagctccac cgcccgcccg gcctcgtagc gcgcgcccgc
     3421 cagccggttc tgcgcccgca gcgcccgcgc cagcagcaaa tgcgggtaag gctcctgcgg
     3481 agccgcggtg agcgcgtcgc gcagcggctc gatcgctccg acgctgttgc ccgcccgcag
     3541 cagcgcttcg gcgcgccgca tcgccaggtc gaacggttgc atggcctcag ccttgctcgc
     3601 gcaggtagcg gcgaagcgcg gcggtgcgat gctgcgacac ataggccgcc gccgcgaaca
     3661 gcaggccgag ggcgagcgcc gcgacgaaga aataatgcgc ctggttcacc cgcagcaggc
     3721 ccgaccggac cgccgcgtca agcgcggcca gcgccgcgaa ataggtgacg atcggtcccg
     3781 ccgacaaggc gatctcacgg ttgcggtgcg ggccattgag cgccaccgca ttagccgcga
     3841 tcagcagcgc gccccacggc aggaagatct gcgcgacgat gaacaccagc agcggcggca
     3901 gcgcgatccg tccgagcggg cccggccgcg gctcgtcgat cagccggtag cgcagcgaat
     3961 cggtcaaagg ccgttcgcct tcaaaaaggc ggcgacatcg tcatactggc cgcccgcatt
     4021 cgaatatttg gcgtaattgc gcgcggtggt cagccattcg agggtggtcg ggcgcacctc
     4081 cgccagcgcc tcgacgaggt ggcgctgcgc gaccgggctc tccacgccgg aggcgagtgc
     4141 gtcctcgatc gcgaggtcga ccgcggtgtt gacgaggttt tcgaggtcgg cgcccgaata
     4201 gccggcggtc ttggcggcga tcgcctccac gtcgcaatcg tcgccaagcg ggcggccgct
     4261 cagctggagc cgcaagatcg cggcgcgggc gccgcgatcc ggcggcggca cgaactgaac
     4321 gcgatcgaac cggccggggc ggcggaaggc ggaatcgacg ccccacggca tgttggtcgc
     4381 cgccaggatc agcacgcctt cattattgtg cgcaaagccg tccatttcgg cgaggaaggt
     4441 cgataccaga ctgatctggc tctggttggc attgccgctg cgcgagccgg cgagcgcctc
     4501 caattcgtcg aagaacagga cggtgggcgt gtcgcggcgc gcgtcggcga agatcgcggc
     4561 gagcttgcgc tcggcctcgc cgatatattt gtcgaccaca tcgaccaccg gcacgttgac
     4621 gaagcgggcg tcgcattcgc ccgcggtcgc gcgcgccagc aatgtcttgc cgcagccggg
     4681 agggccgaac agcagcacgc cgccgcccgc cttgcgccgg tatttggcga acaaagacgg
     4741 cttcctgaac ggcgtcacga tccgccgcgt gatctgcttc ttgacctcgt cgagcccgcc
     4801 aacgtcggcg aagccgatcc cggtggtttc cgcgaacagc gcccgcgccc cggcttcctc
     4861 ctcgcggtcg ctgcgttcgg cgccgcgcag cccgcgcgcg gcctgggcga acccgatcac
     4921 gttggaaacc atgcccggct cggcggcggc cttggcttcc gccagccgct cgtcgagggc
     4981 gggatcctcg agcaccggat cggcggcgac ggcggcgcga tagaccgact ccgcctccgc
     5041 cacgcggccg gcgacgagca gggcatcgac gcggatcggg agcaaggtcc gctcgtcgcc
     5101 gcaaagccgg atcgcatcga gcgcgtcgcc gaggtcgagc gccgccgacg ccagccgctc
     5161 gcgcaacccg aggtcaagcg cggcatcggg cgcctcgccc gccgcggcga tcaccgcgcg
     5221 ctggtcggcg tcgccggcgg ccagatcgag cgccgcgctc agcatgcgcg catccccgcc
     5281 cgctcgaaac gcggccagca aagcctcaat ggccggattg atcgtcaaga catttcccct
     5341 tgatttgcct gcataaccgc gccctccggg ccgggcaacg cccggctgag ggcactgaag
     5401 ccgtcagatg atttgaggtc ggcggcagcg acgcggccgc aatgccgtca gaacaaggcc
     5461 ccggccgcat tgtgggcggc ggattcgagc agcgggatcg agaaatagcc gagcggggac
     5521 acgaacaagg cggcggcgac gatgagcgcg ccgccgagcc ggtcggggct gcgctcataa
     5581 gcgggggcgg gatcgtcgaa atacatgatc ttgatgatct tcagataata ataagcgccg
     5641 atcaccgagg tggcgatgcc gactgcggcg agccaggtga ggtcagccgc caccgcagcg
     5701 ttgaacacca ggaatttggg ccagaagccg aacaaaggcg ggatgccggc gaggctgaac
     5761 atgaacgccg ccatcgccgc ggcgaggccg gggcgggtgc gcgagaggcc ggacaaggag
     5821 acgatggtct cgaccggccg gccctcggcg tcgcgcatgc gcagcacgac gaggaaggtg
     5881 ccgagcgtca tcacgacata gacagccatg tagaacagca ccgaggcgac gccttcgcgg
     5941 gtggcggcgg cgaggccgat caaagcgaag ccgatattgt tgatcgagct gtaggcgagc
     6001 agccgcttga tgttgggctg gccgatcgcg gcgacgccgc cgaggatggt cgaggccaag
     6061 gccatgaaga tgacgatctg gcgccattcg aaggtggcgg ggcccatgcc ctcgatcgca
     6121 acgcgggtga gcagcgccat cgcggcgacc ttgggggccg aggcgaagaa ggcggtgacc
     6181 ggcgtcggcg cgccttcata gacgtcgggc gtccacatgt ggaacggcac cgccgagacc
     6241 ttgaaggcga tgccggccat gacgaagacg aggccgaaca attcgccggt ggagacgccg
     6301 tcgcggccca tggcgaggct gatgtcgccg aacagggtcg agccggtgaa gccgtagagc
     6361 agggagacgc cgtatagcag gatgccgctg gcgagcgcgc cgagcacgaa atatttgagg
     6421 ccggcctcgg ccgagcgggt gtcgcggcgc tggaaggagg cgaggacgta ggccgacagg
     6481 ctctgcagct cgagcccgac atagagggtc aggaggtcgc cggcggagac catcatgccc
     6541 atgccgacgc cggagagcag gatcagcacc ggatattcgg cgcggaacac gccgtcgcgg
     6601 gcgaaccagc cctgcgccgc gaccagggcg acggcggcgg cgatgtagat caggatcttg
     6661 gagaaggccg cgaagctgtc ggcgacgtag aggccgtcga agccgaccgc gccggcgtcg
     6721 cggatgccgg ggatgaacaa ggcggcgagc aggaacgccg ccaccgccag ccaggtgacg
     6781 aggcgcgagg ccttgtcgcc gacgaacgcg gccaccatca gcagggcgag cgagctcagg
     6841 ctgaggatga tttccgggag ggtgaggagg agggaattac ccacgtctga taactccccg
     6901 ttcgtgtcga gcccttcgac gccgcctgcg gcggcgctct ggatgaactc cgtcgagaca
     6961 ccctctcgag cgaaggcgag aacggtgcct cggcttcgct cggcagggtc cctcgacttc
     7021 gctcgggacg aacggattgc accgcagcat caatgcgctc ccgcttcggc ggcggccggg
     7081 gcggcgtggc tgccgctgtc gccggtcggg cgggcggcgc ggcccatggc gggggcggag
     7141 tcgctcggcg gcgccgcgcg ctcgatccgg gcgagcaggg tgccgacgtc gttacgcatc
     7201 ggggcgagga agctttccgg atagacgccc atccacagca ccacggcggc gatcggggcg
     7261 aggatgaaga attcgagccg ggtgagatcg ggcatggcgg cggcgtcggg attacgctgg
     7321 gtgccgtagg cgacgcgcca atagaggtag agcatatagg ccgcgccgag gatgatgccg
     7381 gtggtggcga cgaccgcgac caaagtgttg gcgcggtagg cgccgatcag gctcaggaac
     7441 tcgccgacga agccggcggt gccgggcagg ccgatcgaag ccatggtgaa gagcaggagc
     7501 agcaccgcat aagccggcat gttgtcggac aggccgccat aacggtcgat gtcgcgggtg
     7561 tggagccggt cgtagaccac gccgacgcac aggaacaaag cgcccgagac gaggccgtgg
     7621 ctgagcatca ccatcagccc gccctcgatg ccctggcggt tgaaggcgaa caggccgatg
     7681 gtgacgaagg ccatgtgcgc caccgaggaa taagcgatca gcttcttcat gtcctgctgc
     7741 acaagcgcga ccagcgaggt gtagacgatc gccaccatcg acaggcccat gaccagccag
     7801 aacagctgcg ccgaggcttc ggggaacatc ggcagcgaga agcggatgaa gccgtagccg
     7861 cccatcttca gcagcacgcc ggcgaggatc acggagccgg cggtcggcgc ctggacgtgg
     7921 gcgtcgggca gccaggtatg gaccggccac atcggcatct tgaccgcgaa cgaggcgaag
     7981 aaagcgagga acagccaggt ctgcgccgcg gccgggaaat ccgtcgccat cagccgcggg
     8041 atgaaggtct cgccgccggc atggttcacc atccacagca tcgccaccag catcagcacc
     8101 gagccgagca gggtgtagag gaaaaatttg taggaggcgt agatgcgctg ggcgccgccc
     8161 cagatgccga tgatcaggaa catcgggatg aggccggcct cgaacatgat gtagaagagg
     8221 aagatgtcct gggcgacgaa cacgccgatc atcagcgcct ccatgaggag gaagctcgcc
     8281 atatattcgg ggatgcggcg gtcgatcgac gaccaggagg cggcgatgca gaaaggcatc
     8341 aggaagacgg tgagcaggat cagcatcagc gcgatgccgt cgatgccgag cgcccaggcg
     8401 aagcggccga agaccggctg atattcctgg aactgccatt gcgggccgcc gatatcgtag
     8461 ctgaaccaca aggcgatgcc gagcgcgagc tcgagcgcgg tggtccacag agcgagccag
     8521 cgggccgagc tgtcgccgag cttcaggcag atcagggcgg cgatcagcgg cacggccagc
     8581 atcaggctga ggatcgggaa gccgagcgcg ttcacgatag ctcctccccg gtacggatca
     8641 tcggattggc catgccccgc atggccaagg atcgtccggg ggacgatccg acccgatgat
     8701 gggtaccgct cgaagagcgg tggagggggg cgagcgacgc gagcatggag aggtgggccc
     8761 cctccaccac gccgcttcgc ggcgcggtcc ccctccccgt tccggggagg attttaggaa
     8821 cgcacctcat cgcgccatcg cccaggtggc ggcggcggcg aggccgagca gcatcaccaa
     8881 tgcataagtg tagagatagc cggactggag ccgcgcggtg agcttgttgc cgcccgaaac
     8941 cagagcggcg gcgccgttgg ggccgaagcg gtcgatcgtg ccttcatcgc cgcgcttcca
     9001 gaacagccgg ccgaaccaga gcgagggacg gatgaagacg aggtcgtaaa tctcgtcgaa
     9061 ataccatttg ttgagcagga agccgtggag gacgcggaac tgctcgacga agcgctccgg
     9121 gaagctggtg tggcggacat aggcctggag cgcgatcagg aagccgacga tcatgacgag
     9181 gccgggcagc agcttcaccc acagcggcac gtgatggatt tcctccatca gatgggcgtc
     9241 gaaggcgagg ctgccgcgcc agaattccac gccttcgtgc gcgtcgatga aaggcccgtg
     9301 gaacagcagc ccggcgaaga tggcgccgac cgacagcacc cccagcggca ccagcatggt
     9361 ccacgggctt tcgtgcgggt ggtagccgcc ggtgccggcg gggacctgcc gtgccgaggc
     9421 ttcgcccggg cgggtgtcgt ggccggcatg ctcgtcctcg gggtgcggat cggcctcatg
     9481 atcgtggctg cggccgtgat cggcatcgtg gagcgcatgc tggatatggc ccgaggcggc
     9541 ccagcgcggc ttgccgaaga aggtgaggaa gatgagccgc cagctgtaga agctggtcag
     9601 caaggccgcg aagatgccga ccgtgaaggc gacgccgccg atggtggtgc cgctggcgaa
     9661 ggcgctctcg atgatcgcgt ccttcgaata gaaaccggcg aagccgaagc cggcaaagcc
     9721 gccgacttgg atgatgccga cgccggtgat ggcgagggtg ccggccagca tcgccgagaa
     9781 agtgagcggg atcgacttac gaaggccgcc gtagaagcgc atgtcctgct cgtggtgcat
     9841 ggcgtggatg accgagccgg cgccgaggaa cagcaaagcc ttgaagaagg cgtgggtgaa
     9901 gaggtggaac atggcggccg aataggcgcc gacgccggcc gcgaagaaca tgtagccgag
     9961 ctgggagcag gtcgaatagg cgatcacgcg cttgatgtcg ttctggacgg tgccgacggt
    10021 ggcggcgaac aaagcggtgg cggcgccgat gaaggtgacc acgcccaatg cggtctggct
    10081 ggtctcgaac atcggcgaca ggcgacagac catgaacacg ccggcggtca ccatggtggc
    10141 ggcgtggatg agggcggaga ccggggtcgg gccttccatg gcgtcgggca gccaggtgtg
    10201 gaggccaagc tgcgccgatt tgcccatcgc gccgatgaac agcaggatgc agagcaaggt
    10261 catggtgtcg acgcggtagc cgaggaagcc gatggtcgag ccggccatgc cgggcgcggc
    10321 ggcgaggatg tcggggatcg agacggtgcc gaacaccagg aaggtgccga agatgccgag
    10381 gctgaagccg aaatcgccga cgcggttgac cacgaaggcc ttcaaggcgg cggcgttggc
    10441 ggagggcttg tggtaccaga agccgatcag gaggtaggaa gcgaggccga cgccttccca
    10501 gccgaagaac atctggacga gattgtcggc ggtcaccagc atcagcatcg cgaaggtgaa
    10561 cagcgacaga taggcgaaga agcgcggctg gctgtcgtcc tcggccatat agccccagct
    10621 gtagaggtgg acgagcgccg agacggtggt gacgacgacc agcatcaccg cggtcagcgc
    10681 atcgacgcgc agcgaccagt cgacgcgaag gtcgcccgaa tggatccagg ccagcaccgg
    10741 ctcgacccag ccgccctgat aattgccggc catgaagccg aggaagatcg gccaggacaa
    10801 agcgcaggag gcgaacagag cgccggtggt gatgagcttg gcggcagtgt tgccgagcgc
    10861 gcggttgcca agaccggcga cgatcgcggc gaggagcggc aggaagacga tgagctggat
    10921 catgacgccc ccaccagggc gtcaccctga acttgtttca gggtccatgt tcccgccgca
    10981 gtgcggcccg caaatggatg ctgaaccaag ttcagcatga cgaaggagcg catcccctac
    11041 cccttcatct ggttgatgtc gtcgaccgcg atcgtgccgc ggccgcggaa gtagatgacc
    11101 aagatggcga gaccgatcgc cgcctcgccg gcggcgacgg tgaggacgaa catc
//
LOCUS       DASVRK010000263         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_299118, whole genome shotgun sequence.
ACCESSION   DASVRK010000263 DASVRK010000000
VERSION     DASVRK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299118"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VF589_10520"
     CDS             <1..242
                     /locus_tag="VF589_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258057.1"
                     /translation="ALLSRWRRGRAPAAVRAPEPPPALSASLDAALAARRARRPALAE
                     RARKAARTRIHNRLVRDALAARHLNTTEGDEHGRS"
     gene            229..465
                     /locus_tag="VF589_10525"
                     /pseudo
     CDS             229..465
                     /locus_tag="VF589_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GapR family DNA-binding domain-containing
                     protein"
     gene            598..846
                     /locus_tag="VF589_10530"
     CDS             598..846
                     /locus_tag="VF589_10530"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX8258058.1"
                     /translation="MPLGETDGGKTGADAPPGYDEAVAAVVEHGKASTSWLQRHFRIG
                     YNPAAAIMERMQREGIVSAPDAAGRRTVLRTDHDRDAA"
     gene            843..1130
                     /locus_tag="VF589_10535"
     CDS             843..1130
                     /locus_tag="VF589_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258059.1"
                     /translation="MKFAPHPGATRRFGPPADWDPELHGPCGTLEVADIVRDTPDGGQ
                     LFMESLWRPEPEDLAALNAGAAIVLGIRGCTHPVVYVGVTSAPKHDGGGGR"
     gene            1127..>1838
                     /locus_tag="VF589_10540"
     CDS             1127..>1838
                     /locus_tag="VF589_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX8258060.1"
                     /translation="MTGILSSRDHLRTFRHAHLFSGIGAGAKGLNLASPRVGNLRGEF
                     QCAGGIDVDPGAIANFERMTGVKGRVMDLFDRAQYASFWGREPPAGWREASPLDVWSA
                     FGRYIDVAFTSAPCKGFSGLLSATKSKTDKYQALNRLTVRGMWLLLEAYRDDPVPIIL
                     FENVPRIMTRGRPLLAEITQLLRGYGYIVNEDVHDCGEIGNLAQTRKRFLLIARHPVK
                     IPPFVYQPPKHRLRGVGEV"
BASE COUNT          292 a          675 c          618 g          253 t
ORIGIN      
        1 ccgccctcct ctcccgctgg cggcgcggcc gcgctccagc cgccgtgcgc gcgcccgaac
       61 cgccgccggc gctcagcgcc tcgctcgacg cagctctggc ggcgcgccgc gcccgccggc
      121 cggcgctggc tgagcgcgcc cgcaaggctg cgcgcacccg aatccataac cgcctggtcc
      181 gcgacgcact cgccgcccgg cacctcaaca caaccgaagg agatgaacat ggccgcagct
      241 agcattgccg ccgacgaact gcgcctcctc atcgagcgca tcgaacgcct cgaggaggaa
      301 aagcaggcga tcgccgacga cgtgaaggac gtctacaagg aggcgaagtc ccgcggctac
      361 gacgccaaga ccatccgcgc gctgatccgg ctccggaaga tggagacgca cgatcggcag
      421 gaagccgagg cgatgctcga cacctacaag gccgcgctcg gcatgaccga cttcggctcg
      481 acgccgctcg gctcggcggc cgccagcgat gacgacatgc cgctggccga gctggcgctg
      541 cggctgcggc ggatgggcgc caccatcgaa ctcgtcgacg gccggcgcgt gctcaacatg
      601 ccgctcgggg agacggatgg gggcaagacc ggggccgacg cgccgccggg ctatgacgaa
      661 gcggtcgccg ccgtcgtcga gcacggcaag gcatccacct cctggctgca gcggcacttc
      721 cgcatcggct ataaccccgc cgccgccatc atggagcgga tgcaacgcga aggcatcgtc
      781 agcgctcccg acgcagccgg ccggcgcacg gtgctgcgca cggaccacga tcgggacgcg
      841 gcatgaagtt cgcccctcac cctggcgcga cgcgccgctt cggcccgccc gccgactggg
      901 acccggagct tcacggcccg tgcggcacgc tcgaggttgc agatatcgtc cgcgacacgc
      961 ccgatggcgg ccagctgttc atggaaagcc tgtggcggcc ggagcccgag gatctggcgg
     1021 cgctgaacgc cggcgcggcg atcgtgctcg gcatccgcgg ctgcacgcat ccggtcgtct
     1081 atgtcggcgt cacctcggcg ccgaagcatg acggtggagg cggccggtga cgggcatcct
     1141 ctccagccgg gatcatctgc ggaccttccg ccacgcccac ctcttctcgg gcatcggcgc
     1201 aggggcgaag gggttaaacc tagccagtcc gcgcgtcggc aacctcaggg gggagttcca
     1261 gtgcgccggc ggcatcgacg tcgatcccgg cgccatcgcc aatttcgagc ggatgacggg
     1321 cgtcaagggc cgcgtcatgg acctgttcga ccgcgcccaa tatgcttcct tttgggggcg
     1381 ggagccgccg gccggctggc gcgaggcctc gccgctcgac gtctggtcgg cgttcggccg
     1441 ctacatcgac gtcgccttca cctcggcgcc ctgcaagggc ttttccgggc tgctctcggc
     1501 gacgaagtcg aagacggaca aatatcaggc gctcaaccgg ctgaccgtgc gcggcatgtg
     1561 gctgctgctc gaagcctatc gcgacgatcc ggtgccgatc atcctgttcg agaatgtgcc
     1621 gcgcatcatg acccgcggcc ggccgctgct ggcggagatc acccagctgc tgcgcggcta
     1681 cggctacatc gtcaatgagg acgtccacga ctgcggcgag atcggcaacc tcgcccagac
     1741 ccgcaagcgc ttcctgctga tcgcgcgcca cccggtgaag atcccgccgt tcgtctacca
     1801 gccgcccaag caccggctgc gcggcgtcgg cgaggtta
//
LOCUS       DASVRK010000264         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_489135, whole genome shotgun sequence.
ACCESSION   DASVRK010000264 DASVRK010000000
VERSION     DASVRK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489135"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..465)
                     /locus_tag="VF589_10545"
     CDS             complement(13..465)
                     /locus_tag="VF589_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX8258061.1"
                     /translation="MIVFDLRCAPAGHVFEAWFGSTGDYEAQRARGLVECPLCGVKAV
                     EKAVMAPNVGAKGNRSGGEPAVPVSSDPEGVKAMLAAVAAAQRKLLQGSEFVGDRFAE
                     EARAIHVGDADKRSIHGRATREQSESLAAEGIAIAPLPLPVVEPREEN"
     gene            complement(462..1292)
                     /locus_tag="VF589_10550"
     CDS             complement(462..1292)
                     /locus_tag="VF589_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8258062.1"
                     /translation="MRIALYQAQTGIDPAANAAALVAAVEQAAAGGAAMLFTPEMSGL
                     LDKDRERAAAQLRPEREDPVLAAVREAAARVGLWVHLGSLALAGERADGRLVNRGFVI
                     GADGEIRARYDKIHLFDVDLPTGESWRESAAYAGGERAMVAETPLGRLGLSICYDLRF
                     PDLYRALTDAGATILSIPAAFTVPTGQAHWHVLMRARAIEAGAFVVAAAQTGRHEDGR
                     ETFGHSLAIGPWGEVLLDMAETPGLGFAELDLDQVEAVRRRLPAIRHRRPIGEPELAS
                     "
     gene            complement(1293..1550)
                     /gene="grxC"
                     /locus_tag="VF589_10555"
     CDS             complement(1293..1550)
                     /gene="grxC"
                     /locus_tag="VF589_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX8258063.1"
                     /translation="MPRVEIYTKFFCPYCARAKQLLERKGVEYDEYEVSLGGEKKVEM
                     MQRANGRHTVPQIFIDGRHIGGSDDLAELDRSGKLDPLLQS"
     gene            complement(1599..2348)
                     /locus_tag="VF589_10560"
     CDS             complement(1599..2348)
                     /locus_tag="VF589_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX8258064.1"
                     /translation="MTPAITRAARSAVREIVDFALPPRCPGCGTVVTDQHSFCLGCWQ
                     KLDFLGEPCCSRCGLPFDYDGGADAECGGCLADPPQFDRLRAAVAYGEVARHVALKLK
                     YSGRPGVVETLAHFMRRHVTAEDAEAILAPVPLHRWRIWKRGYNQSALIVSALARRTG
                     IAADLDLVRRVKATPPLKGKGRRERALAVRGAFRVEERAKALVGGRRVILIDDVYTTG
                     ATANACAKVLRRAGAASVNVICWARVVRVED"
     gene            2392..>3039
                     /locus_tag="VF589_10565"
     CDS             2392..>3039
                     /locus_tag="VF589_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8258065.1"
                     /translation="MDADKPFDRRLRRLRRDRAARGFADYLHRHAAEELLERLDLVKR
                     DFAAALDLGTGDGRLAARLRERGIAVTAADPGYAFAARAGGVQCDEDRLPFADGSFDL
                     VTSVGTLDGVNDLPGALVLIRRALRPDGLFLAAFAGAGSLPRLRAAMAAADEAQGAAS
                     AHIHPQIDVRAAGDLLMRAGFALPVVDTDTIAVRFPHLIALVRDLRAMAATNILAS"
BASE COUNT          422 a         1132 c         1053 g          432 t
ORIGIN      
        1 ccggcgaccg ggtcaattct cctcgcgcgg ctcgaccacc ggcagcggca gcggcgcgat
       61 cgcgataccc tcggcggcga ggctctcgct ctgctcccgc gtggcgcggc catggatcga
      121 acgcttgtcg gcgtcgccga cgtggatcgc ccgggcctcc tcggcgaacc ggtcgccgac
      181 gaactcagag ccctggagca gcttccgctg cgccgcggcg acggccgcca gcatcgcctt
      241 gacgccttcc gggtcggagg agaccggcac ggccggctcg ccgccgctgc gattgccttt
      301 ggcgccgaca ttgggcgcca tcaccgcttt ctcgaccgcc ttcacgccgc agagcgggca
      361 ttcgaccagc ccgcgcgcac gctgcgcctc ataatcgccg gtggatccga accaggcttc
      421 gaagacatgg ccggcgggcg cgcagcgaag gtcgaagacg atcacgatgc gagctcgggc
      481 tcgccgatcg gccggcggtg gcggatcgcc ggcagccggc gcctcaccgc ctccacctgg
      541 tcgaggtcga gctcggcgaa accgaggccc ggcgtctctg ccatgtcgag cagcacctcg
      601 ccccacgggc cgatcgccag cgaatggccg aacgtctcgc gcccgtcctc atgccgcccg
      661 gtctgcgccg cggcgaccac gaacgcaccg gcctcgatcg cccgcgcgcg catgaggacg
      721 tgccaatgcg cctgcccggt cggcaccgtg aaggcggcgg ggatgctgag gatcgtcgcg
      781 ccggcgtcgg tgagcgcccg atagagatcg ggaaagcgca gatcgtagca gatcgacagg
      841 ccgagccgtc cgagcggggt ttcggccacc attgcccgct cgccgccggc ataagcggcc
      901 gattcgcgcc agctctcgcc ggtcggcaga tcgacgtcga acaggtggat cttgtcgtag
      961 cgggcccgga tctcgccgtc ggcgccgatc acgaagccgc gattgaccag cctgccgtcc
     1021 gcgcgctcgc cggccagcgc cagcgatccg agatgcaccc acaggcctac cctcgccgcc
     1081 gcctcgcgaa ccgcggccag caccggatcc tcgcgctcag gccggagctg cgccgccgcc
     1141 cgctcgcgat ccttgtcgag caggccgctc atctcgggcg tgaacagcat cgccgcgccg
     1201 cccgccgccg cctgctccac ggcggcgacg agagcggcgg cattggcggc cggatcgatc
     1261 ccggtctgcg cctggtagag cgcgatccgc attcagcttt gcagcagggg atcgagcttg
     1321 ccggaacggt cgagctcggc aagatcgtcg gagccgccga tatggcggcc gtcgatgaag
     1381 atctgcggca cggtgtggcg gccattggcg cgctgcatca tctcgacctt cttctcgccg
     1441 ccgaggctga cttcatattc gtcatattcg acgcccttgc gctcgagcaa ttgcttggcc
     1501 cgggcgcagt aggggcaaaa gaatttggtg tagatttcga cccgcggcat ccgtggctcc
     1561 tccgttccgc ccagacctcc gcacccgcgg cccatttgtc aatcttcgac ccggacaacg
     1621 cgtgcccagc agatcacgtt cacgctcgcg gcgccggccc ggcgcagcac cttggcgcag
     1681 gcattggcgg tggcgccggt ggtgtagacg tcgtcgatca ggatcacccg gcggccgccg
     1741 accagcgcct tcgcccgctc ctcgacgcgg aaggcgccgc gcacggccaa ggcccgctcg
     1801 cggcggccct tgcccttgag cggcggcgtc gccttcaccc gccgcacgag gtcgagatcg
     1861 gcggcaatgc cggtccgccg cgcgagcgcg gagacgatca gcgccgactg gttatagccg
     1921 cgcttccaga tccgccagcg atggagcggc accggcgcca ggatcgcttc ggcgtcctcg
     1981 gccgtgacgt gccggcgcat gaagtgggcg agcgtttcga cgacgccggg ccggccgcta
     2041 tatttgagct tcagggcgac gtgacgggcg acctcgccat aagcgaccgc cgcccgcagc
     2101 cggtcgaact ggggcggatc ggcgaggcag ccgccgcatt cggcgtcggc gccgccgtca
     2161 tagtcgaacg gaaggccgca gcggctgcag caaggttcgc cgaggaaatc gagcttctgc
     2221 cagcagccga ggcagaagct atgctgatcg gtcaccacgg ttccgcagcc ggggcagcgc
     2281 ggcggcagcg cgaaatcaac gatctcgcga acggcgctgc gggcggccct ggtgatggcg
     2341 ggcgtcacgc cccccttgtg tcccgccggc cccggccgca caagcccggc cgtggacgcc
     2401 gacaagccct tcgaccgccg cctgcgccgc ctccgccgcg accgggccgc gcgagggttc
     2461 gccgactatc tccatcgcca cgccgccgag gagctgctcg agcggctgga cctggtgaag
     2521 cgcgacttcg ccgcggcgct cgaccttggc accggagacg ggcggctggc ggcgcgcctg
     2581 cgcgagcgcg gcatcgcggt gaccgcggcg gacccgggct atgctttcgc ggcccgggcg
     2641 ggcggcgttc aatgcgacga ggaccgcctg cccttcgccg acggctcgtt cgacctggtg
     2701 acctcggtcg gcacgctcga cggcgtcaac gacctgccgg gcgcgctggt cctcatacgg
     2761 agggcgttgc ggccggacgg gctgttcctg gccgccttcg ccggcgccgg gagcctgccg
     2821 cggctgcgcg cggcgatggc ggcggccgac gaggcccagg gcgccgcctc ggcccacatc
     2881 catccgcaga tcgacgtccg cgccgccggc gacctgctga tgcgcgccgg cttcgcgctg
     2941 ccggtggtcg acaccgacac gatcgccgtt cgcttcccgc atctgatcgc gctggtccgc
     3001 gacttgaggg cgatggccgc gaccaacatc ctcgcctcg
//
LOCUS       DASVRK010000265        10311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_489416, whole genome shotgun sequence.
ACCESSION   DASVRK010000265 DASVRK010000000
VERSION     DASVRK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489416"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VF589_10570"
     CDS             <1..1323
                     /locus_tag="VF589_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8258066.1"
                     /translation="WGVPIALYVHRKTGEYLKDEGVNARILAAFEAGGADAWFGADHQ
                     ALLGNEYSLAEWEPVGDILDVWFDSGSTHAFVVEKRYGEGVRADLYCEGSDQHRGWFQ
                     SSLLESCGTRGLAPFKSVLTHGFTLDANGRKMSKSVGNVVDPLKIIDESGADILRLWV
                     ASTDYFEDVRIGKEILAGTSDAYRKLRNTFRYLLGALSDFSEAERVAPGDMPELERWV
                     LHRLAMLDAELRSAAEDFEFNRYARALTAFANDDLSAFFFDIRKDSLYCDAHHALKRR
                     AYRTVLDEVFHALIRWVSPILCFTAEEVWASRYPDAGSVHLETWPAIDAGWLDPALGD
                     KWDLLRALRTRVTEAIEPMRRDKIIRSSLEAAVDLALAEPDLLDAVRSVEFQELCIVS
                     ELHAAAGEHDRVAVSRTDREKCGRCWRCLPEVTEDGALCARCEEVLNG"
     gene            1316..1933
                     /gene="lspA"
                     /locus_tag="VF589_10575"
     CDS             1316..1933
                     /gene="lspA"
                     /locus_tag="VF589_10575"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX8258067.1"
                     /translation="MAEVTDVSGHPLESEGPASPPPPEVAKPDARARAREAKTGNHRT
                     LGLTLAALIFAFDQFTKYVVTYPLDLRNQPDREINLLSFFDLRWVENRGVSMGFLTAS
                     TDLARWLLVALTAAISAGVIYWMWREKRRDDTIALAFVLGGALGNIVDRIRYGFVVDF
                     ADLHFGEWRPFLIFNVADAAITIGVLLLLFRALLTRDGKAPRKEA"
     gene            1935..2348
                     /locus_tag="VF589_10580"
     CDS             1935..2348
                     /locus_tag="VF589_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HEX8258068.1"
                     /translation="MRIVMLTLAAAPLLLTACGTGILGGSRARPDEFAVARSAPLVVP
                     PDFALTPPAPGQVSPAAGDSRAQAVEALFGGPAPRSAAETQVLQQTEGDRAAAGARSV
                     VGDPGTNVVDKGATTQTILAVPEGDGQEARVETPQ"
     gene            2407..2760
                     /locus_tag="VF589_10585"
     CDS             2407..2760
                     /locus_tag="VF589_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258069.1"
                     /translation="MTHHAPPHLPSASEAGLKVAQLRHVLGLVEQIAGRDSGDGAAEA
                     ALDESARISIAYEGAMPILQRRFDALAGESTAWAAAGVEALLAADGDPSPAAAARLAD
                     ELDRALAKLAAVLGA"
     gene            complement(2908..4188)
                     /locus_tag="VF589_10590"
     CDS             complement(2908..4188)
                     /locus_tag="VF589_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX8258070.1"
                     /translation="MNRTLGIQLPDYPELVSGGAALLIAVAAWAIGSFAARGLAPRLA
                     AWWEQRAGARSEGIAARMGGLVRAATAGILLAIAIEVEPWPDLPEIILGVAAAAAAAL
                     LVVGLIRGLNMPRWVSLLLAGVAFVAIFADVVDGLEPVTRVLDQIGFTAGTRRFSLLT
                     LLQMAVGLLVLFAVVRLISRVVNHSIRRATGLDPTQQLLTQKLVGIGLLVAAFFIGID
                     LVGIDLTALAVFSGAFGLAIGFGLQKTFGNLIAGIILLMDRSIKPGDVIVVGESFGHV
                     TKIGVRAVSIVTRDGKEHLIPNENLMTQEVENWSYSSPNVRVHIPVGVGYDCDLALAQ
                     RLMIEAATAPSRVLKSPRPTVWLRAFGESSVDHEILVWISDPEGGVGNVQSEVLNLLW
                     RLFKENGVALPYPQRDIHIKEWPERGRLDIPDEG"
     gene            complement(4185..5402)
                     /gene="metC"
                     /locus_tag="VF589_10595"
     CDS             complement(4185..5402)
                     /gene="metC"
                     /locus_tag="VF589_10595"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX8258071.1"
                     /translation="MGRGPGHPQGDGARVNGEGAETRVVRAGRRKEWQGRMVNPPVHR
                     GSTILFDSVAELRAAAPGLGTLYYGLHGTPTQWALSEALTALEPGAAGTMLFPSGLAA
                     VTGALFTILSAGDELLMVDSAYGPTRRFCDEILPRYGVSTRYYDPLIGAGIAELIGPE
                     TRAIFMESPGSLSFEVQDVPAICAAAKARGVATLLDNTWATPLLFPALGAGVDLSILA
                     CSKYVGGHADLMLGSVTAGPEWFARLEQTTFDLGHAVSPDDAWLAARGLRTMAVRLRR
                     HEENALTVARWLKDQPRVARVLHPALSDCPGHEIWARDFAGASGLFAFALAGGGEAER
                     TRFIESLELFGIGYSWGGYESLVIPVDLKRIRTATRPEHPGPLVRLHIGLEDPADLIA
                     DLAQALGRLGEPG"
     gene            complement(5357..6199)
                     /gene="sseA"
                     /locus_tag="VF589_10600"
     CDS             complement(5357..6199)
                     /gene="sseA"
                     /locus_tag="VF589_10600"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEX8258072.1"
                     /translation="MESLVSTEWLAGELGAPDLRVIDASIFLPGSGRDGRTEYEAEHI
                     PGAVYMDLEEIVDSSHPAPHMLPPEHKFASRMQTLGLGDGSRFVVYDNSPLHSAARAW
                     WMLRSFGAHYVAILDGGMAKWKAEGRPTESGRPQVRHGHFTPMLDRSAVADKDYVSGI
                     VHSGEHEIVDARPASRFAGEDPEPRAGVSGGHIPGSKSLPQSELFNSDHSWKQGDALR
                     AAFDAAGVDLSRPLVATCGSGVTACALLFGAHLLGKEDVKLYDGSWSEWGADPATPKA
                     TGRA"
     gene            complement(6202..7737)
                     /locus_tag="VF589_10605"
     CDS             complement(6202..7737)
                     /locus_tag="VF589_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX8258073.1"
                     /translation="MNSALSILLFAAGSLALAAPSRAQGDPAPAQKEVAPAPAPPAPA
                     APVPPPTISVTRHSGTFGGQRMRYVATAGETYLKAEDGTPKASIFSIAYVKEPRDPNR
                     PITFLFNGGPGSGSLWLHMGAFGPKRVAIPSDARDDGAPPFPIVDNPDSLLDVTDIVF
                     IDPVGTGFSHALGKTEAKEYWGVTADAKSVAEFIRIWLGSNGRWNSPKFLGGESYGTT
                     RSAAVVNQLEGAFNDVSLNGILLISTILDFGAQANVPGNEMPYVLFLPSMAAAAHHHG
                     KAPADAGTLEAFVEQAREFAIGDYASALLKGQALPAAERASIRQRLARFTGLSERFLE
                     DADLRVSDGRFYKELLRDRGLTIGRLDARYTGRDYDKAGEEPDNDPSFYGIDAGYTAA
                     LNQWVRGGLGYRTDRQYVTIGGVGDWDWNIGKGDLTYYRNVAPYIGKAMRENDDLRIF
                     VGQGYFDFATPFFGAEYSLSRTGIPQDRVEYRYYGSGHMMYVRDEDRVQLSRDIRDFI
                     RRR"
     gene            complement(7814..8464)
                     /locus_tag="VF589_10610"
     CDS             complement(7814..8464)
                     /locus_tag="VF589_10610"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8258074.1"
                     /translation="MAYQPSGPTVAIPVYDQVDMLDVTGVFEMLTWADIGVELLAVDA
                     GTVTFRKGFSFEVATSFDAAAPAYDAIWVPGGDPSALAAIIADPGGPYMSFLRAQAET
                     ARYLASVCEGALLLAAAGLLDGYEATTHWAFIPCLMQAYPNVKVAPGHPRFIHDRDRL
                     TGGGISSGLDEALKLIELLTCTATAQSVQQMTQYYPQPPVTSEIPNTVTCPITGMA"
     gene            complement(8474..10102)
                     /locus_tag="VF589_10615"
     CDS             complement(8474..10102)
                     /locus_tag="VF589_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX8258075.1"
                     /translation="MPTSGTTAVRRSLFDVQTDYDNGNKEELENLMRAWRGIKALPPE
                     DPRSFFVIGGYHGEPFRGPGATDPAYWGGYCEHGTVLFPTWHRAYLHVLEKALQSIAG
                     CENVMLPYWDECSPASRSYGIPAALTWATFELDGEIIANPLRSFILPVALGDQLQGDD
                     SLYSKPAGYETVRYPLSGLVGTPADQAATAAHNAKFPNDATNVGLLNQNVADWLNGMA
                     VVNGKPVGAAVYDRYVSCLNTPTYTLFSNTTSAGAWNKANPQQLVVPLESPHNAVHLA
                     VGGFDVPGQGDFSPIPGANGDMGENDTAGLDPIFFFHHCFIDYAFWTWQKKHGATSAP
                     LTIDYADPGANSSTGTAQGPAAGTPPDTPLTADSPLNPFLMDDGSGGTRPMTTNDVTD
                     IRQLGYDYGFGSLDRFVDASEAALLPGAAASAEGRRAVYVSGIDRSKIRGSFVISAYA
                     NIDGEVRPLGHEAVLSRRHVEGCINCQVHLEARTSFRLPEGAEGAVPPVHVEIRTRTR
                     ILASGTAAGAQTRLEAPALAAFAGDAPLFKAEIQ"
BASE COUNT         1721 a         3549 c         3384 g         1657 t
ORIGIN      
        1 tggggggtgc cgatcgcgct ctacgtccac cgcaagaccg gcgaatatct caaggacgag
       61 ggcgtcaacg cccgcatcct cgccgcgttc gaggcgggcg gcgccgacgc ctggttcggc
      121 gccgaccacc aggccctgct cggcaacgaa tacagcctcg ccgaatggga gccggtgggc
      181 gacatcctcg acgtctggtt cgacagcggc tccacccacg ccttcgtcgt cgagaaacgt
      241 tacggtgagg gcgtccgcgc cgatctttat tgcgaaggct cggaccagca tcgcggctgg
      301 ttccagtcgt cgctgctcga aagctgcggc acccgtgggc tggccccgtt caaatcggtg
      361 ctgacccacg gcttcacgct cgacgccaac ggccgcaaga tgtcgaaatc ggtcggcaat
      421 gtcgtcgacc ccttgaagat catcgacgaa tcgggcgccg acatcctccg cctctgggtc
      481 gcctcgaccg actatttcga ggatgtgcgg atcggcaagg agatcctcgc cgggacctcc
      541 gacgcctatc gcaagctcag gaacaccttc cgctatttgc tcggcgcgct gtcagacttc
      601 agcgaggcgg agcgggtcgc gccgggcgat atgcccgagc tcgaacgctg ggtgctccac
      661 cggctggcca tgctcgatgc cgaactgcgc tcggccgcgg aggatttcga gttcaaccgc
      721 tacgcgcgcg ccctcaccgc ctttgccaac gacgatcttt cggccttctt cttcgatatc
      781 cgcaaggata gcctctattg cgacgcgcac catgcgctga agcgccgggc ctaccgcacc
      841 gtcctcgatg aggtgttcca tgccctcatc cgctgggtct cgccgatcct ctgcttcacc
      901 gccgaggaag tttgggcgag ccgctatccg gacgccggct cggtccacct cgaaacctgg
      961 ccggcgatcg acgccggctg gctcgacccc gcgctcggcg acaaatggga cctcttgcgc
     1021 gcgctccgca cccgcgtcac cgaggcgatc gagccgatgc gccgcgacaa gatcatccgc
     1081 tcgagccttg aggcggcggt cgatctcgcc cttgccgaac ccgatctgct cgacgccgtc
     1141 cggtcggtcg aattccagga actgtgcatc gtttcggaac tccacgccgc cgcgggcgag
     1201 catgacaggg ttgcggtgtc gcgcacggac agggaaaagt gcggccgttg ctggcgctgt
     1261 cttcccgaag tgacggaaga cggcgcgctt tgcgcccgct gcgaggaagt cctgaatggc
     1321 tgaagtcacc gacgtttccg gtcatccgct cgaaagcgag ggtcccgcct cgccgccgcc
     1381 gcccgaggtg gcaaagccgg atgcccgcgc tcgcgcccgt gaggcgaaga cgggcaatca
     1441 tcggaccctc ggcctcaccc tcgccgcttt gatcttcgcc ttcgaccaat tcaccaaata
     1501 tgtcgtcacc tacccgctcg acctgcgcaa ccagcccgac cgcgagatca acctgttgag
     1561 cttcttcgac ctgcgctggg tcgagaatcg cggcgtctcg atgggcttcc tcaccgccag
     1621 caccgacctg gcccggtggc tgctggtcgc gctcaccgcc gccatctccg ccggcgtcat
     1681 ctactggatg tggcgcgaga agcgccgcga cgacaccatc gcgctggcgt tcgtgctcgg
     1741 cggcgcgctc ggcaatatcg tcgaccgcat ccgctacggc ttcgtcgtcg acttcgccga
     1801 cctccatttc ggcgaatggc ggccattttt gatcttcaat gtcgccgatg ctgctattac
     1861 catcggcgtt ctgctattgc tgttccgggc gctgttgacg cgcgacggca aagcccctcg
     1921 gaaggaagcc tgaaatgcgt atcgtgatgc tcaccctcgc ggccgcgccg ctgctgctca
     1981 ccgcctgcgg gaccggcatc ctcggcggct cgcgcgccag gcccgacgaa ttcgccgtcg
     2041 cccgctccgc gcctttggtg gtgccgcccg atttcgcttt gaccccgccc gcgccgggcc
     2101 aggtcagccc cgccgccggc gacagccgtg cccaggcggt cgaagccctg ttcggcggcc
     2161 ccgccccgcg cagcgccgcc gagacccagg tcctgcagca gacggaaggc gaccgcgccg
     2221 ccgccggcgc ccgctcggtg gtcggcgacc ccggcaccaa tgtcgtcgac aagggcgcca
     2281 ccacccagac catcctcgcc gtgcccgaag gcgacggcca ggaagcccgc gtcgaaacgc
     2341 cgcaataagc cgcgccggca cggccccgtt agcgcttcat tagccgggcg gcgctagctt
     2401 cggcgcatga cccaccatgc gcccccgcat ttgccgtccg cgagcgaggc ggggctcaag
     2461 gtcgcgcaat tgcgccacgt gctcggcctc gtcgaacaga tcgccggccg cgacagcggc
     2521 gacggggccg ccgaggccgc gctcgacgaa agcgcgcgga tcagcatcgc ctatgaaggc
     2581 gcgatgccga tcctccagcg ccgcttcgac gcgctcgccg gcgaaagcac cgcctgggcc
     2641 gcggccggag tcgaagcgct gctggcggcc gacggcgacc cttcccccgc cgccgccgcc
     2701 cgcctcgccg acgaactcga ccgcgccctc gccaagctcg cggcggtgct cggcgcctga
     2761 accgtcatgc cagcgaaagc tggaatcact tttgttgggt tcgacggaac gggaacccca
     2821 cgtcattcca gcgcaagctg gaatctccct tcttcttccc cggcttccga agagaagtga
     2881 gatgccagct ttcgccggca tgacggttca tccctcgtct ggaatatcta accggccccg
     2941 ctccggccat tccttgatgt ggatgtcgcg ctgcggatag gggagagcga ccccattctc
     3001 cttgaacagc cgccacagca ggttcagcac ttccgactgg acattgccga cgccgccctc
     3061 ggggtcgctg atccagacca atatctcatg gtcgactgaa ctctccccga acgcccgcag
     3121 ccacacggtc ggcctcggcg acttcagcac ccgcgacggc gccgtcgccg cctcgatcat
     3181 cagccgctgg gcgagcgcaa ggtcgcaatc gtagccgacg ccgaccggga tgtgcacgcg
     3241 cacgttcggg ctcgaatagg accagttctc gacctcctgg gtcatcagat tttcgttcgg
     3301 gatcagatgc tccttgccgt cgcgcgtgac gatcgagacg gcgcgcacgc cgatcttggt
     3361 gacatggccg aagctctcgc cgaccacgat cacgtcgccc ggcttgatcg agcggtccat
     3421 cagcaggatg atgcccgcga tcaaattgcc gaacgtcttc tggaggccga agccgatggc
     3481 gaggccgaac gcgcccgaga acaccgcgag cgcggtgagg tcgatgccga ccaggtcgat
     3541 gccgatgaag aaggcggcga ccagcagccc gatgccgacc agcttctgcg tcagcaattg
     3601 ctgggtggga tcgaggccgg tggcgcggcg gatcgaatga ttgacgaccc ggctgatcag
     3661 gcggaccacc gcgaacagga cgagcagccc gaccgccatc tgcagcagcg tgagcaggga
     3721 gaagcgccgg gtcccggcgg tgaagccgat ctggtcgagc acccgcgtga ccggctcgag
     3781 gccgtcgacc acgtcggcga agatcgcgac gaaggcgacc ccggccagca gcagcgacac
     3841 ccaccgcggc atgttgaggc cgcggatcag gccgaccacc agcagcgccg ccgccgccgc
     3901 cgccgccacc ccgaggataa tctccggaag gtcgggccac ggctcgacct cgatcgcgat
     3961 ggcgagcagg ataccggccg tggccgcgcg caccaggccg cccatgcgcg cggcgatgcc
     4021 ttcgctgcgc gcccccgccc gctgctccca ccatgcggcg agccgaggcg cgagcccgcg
     4081 cgcggcgaag ctgccgatcg cccacgccgc caccgcgatc agcaaagcgg cgcccccgga
     4141 caccagctcg ggataatccg gaagctggat gccgagcgtg cggttcatcc cggctccccc
     4201 agccggccga gggcttgggc gaggtcggcg atcaggtcgg ccgggtcttc gaggccgata
     4261 tggagccgca ccagcggccc cggatgttcg ggccgggtcg cggtgcggat ccgcttgagg
     4321 tcgacgggga tgaccaggct ttcatagccg ccccagctat agccgatgcc gaacagttcg
     4381 aggctttcga tgaagcgcgt gcgctccgcc tcgccgccgc cggccagcgc gaaggcgaac
     4441 aggccggacg cgccggcgaa atcgcgggcc cagatttcgt ggccgggaca atcggacaag
     4501 gcgggatgaa gcacccgcgc gacccgcggc tgatccttga gccagcgcgc gacggtgagc
     4561 gcattctcct catgccggcg cagccgcacg gccatggtcc ggaggccgcg cgccgccagc
     4621 caggcatcgt cggggctgac agcatggccg agatcgaagg tggtctgctc cagccgggca
     4681 aaccattccg gccccgccgt caccgatccg agcatcagat cggcatggcc gccgacatat
     4741 ttgctgcaag caaggatgct gagatcgacg ccggcgccga gcgcgggaaa caagagcggc
     4801 gtcgcccagg tattgtcgag cagggtcgcg accccgcgcg ccttggccgc cgcgcagatg
     4861 gcgggcacgt cctgcacttc gaagctcagg gagcccgggc tctccatgaa gatggcgcgc
     4921 gtctccgggc cgatcagctc ggctatgccg gccccgatca gcgggtcgta atagcgggtg
     4981 gagacgccgt agcgcggcag gatctcgtcg cagaagcggc gggtggggcc gtaggcgctg
     5041 tcgaccatca acagctcgtc gccggccgac agtatcgtga acaaggcgcc cgtcaccgcc
     5101 gcgaggcccg aggggaacag catcgtcccg gccgcgcccg gttcgagcgc ggtcagcgcc
     5161 tcgctcagcg cccattgcgt cggggtgccg tggaggccgt aataaagcgt gcccagcccc
     5221 ggcgccgccg cccttagctc cgcgacgctg tcgaacaata tcgtcgagcc gcgatggacc
     5281 ggcgggttga ccatccgccc ctgccattcc ttgcgccgcc cggcgcggac cacccgggtc
     5341 tctgcgccct cgccgttcac gcgcgccccg tcgccttggg ggtggccggg tccgcgcccc
     5401 attcggacca gctgccgtcg tagagcttca catcctcctt gccgagcaaa tgcgctccga
     5461 acagcaacgc gcaggcggtg acgccggagc cgcaggtggc gaccagcggc ctcgaaagat
     5521 ccactcccgc cgcatcgaag gcggcgcgca gtgcatcgcc ttgcttccag ctatggtccg
     5581 agttgaacag ctcgctctgc ggcagggact tggagccggg gatatggccc ccgcttaccc
     5641 ccgccctcgg ctccggatcc tcgccggcga agcgcgacgc cggccgcgcg tcgacgatct
     5701 catgctcgcc cgaatggacg atgccgctga cataatcctt gtcggcaacc gcgctccggt
     5761 cgagcatcgg ggtgaaatgg ccgtggcgca cctgcggccg cccgctctcg gtcggccgcc
     5821 cctccgcctt ccacttggcc atgccgccgt cgaggatcgc gacataatgg gcgccgaagc
     5881 tgcgcagcat ccaccaggcg cgggcggcgc tgtgcagcgg cgaattgtcg tagacgacga
     5941 agcggctgcc gtcgccgagc ccgagcgtct gcatgcggct cgcgaactta tgctcgggcg
     6001 gcagcatgtg cggcgccgga tgcgaggaat cgacgatctc ctccaggtcc atatagaccg
     6061 cgccggggat atgctccgcc tcatattccg tgcgcccgtc gcggccggag ccgggcagga
     6121 agatgctcgc gtcgatcacc ctcaaatccg gcgcgccgag ctcgcccgcc agccattccg
     6181 tcgaaaccag cgactccatc gtcacctcct gcggatgaaa tccctgatgt cccggctgag
     6241 ttgcacccgg tcctcgtcgc gcacgtacat catgtgcccg gagccgtaat agcgatattc
     6301 gacccggtcc tgggggatgc cggtgcggct cagcgaatat tcggcgccga agaagggcgt
     6361 ggcgaaatcg aaatagccct ggccgacgaa tatgcgcaaa tcgtcattct cgcgcatcgc
     6421 cttgccgata tagggcgcca cgttgcgata ataggtgagg tcgcccttgc cgatgttcca
     6481 atcccagtcg ccgaccccgc cgatcgtcac atattgccgg tcggtgcggt agccgagccc
     6541 gccgcgcacc cactggttca gggccgcggt atagccggcg tcgatgccgt agaagctggg
     6601 gtcgttgtcc ggctcctcgc cggccttgtc atagtcgcgg ccggtatagc gcgcgtcgag
     6661 ccgcccgatg gtgaggccgc ggtcgcgcag cagctccttg tagaagcgcc cgtcgctgac
     6721 gcgcaggtcc gcgtcctcga ggaagcgctc ggacaggccg gtgaaccgcg ccagccgctg
     6781 ccggatcgag gcgcgctccg ccgccggcag cgcctggccc ttgagcaagg ccgaggcata
     6841 atccccgatc gcgaactcgc gcgcctgctc gacgaaggct tcgagcgtgc ccgcgtcggc
     6901 cggcgccttg ccgtgatggt gcgccgccgc cgccatcgac ggcaggaaca gcacataggg
     6961 catctcgttg cccggcacgt tcgcctgcgc gccgaaatcg aggatggtcg agatcagcag
     7021 gatgccattg agcgagacgt cgttgaacgc gccttcgagc tggttcacca ccgcggccga
     7081 gcgggtcgtg ccgtagctct cgccgccgag aaatttgggc gagttccagc ggccgttcga
     7141 gccgagccag atgcggatga actcggcgac cgacttggcg tcggcggtga cgccccaata
     7201 ttccttggcc tcggtcttgc ccagcgcatg gctgaagccg gtgccgaccg gatcgatgaa
     7261 cacgatgtcg gtgacgtcga gcaggctgtc gggattgtcg acgatcggga aaggcggcgc
     7321 gccgtcgtcg cgggcgtcgg acgggatcgc cacccgcttc ggcccgaacg cgcccatgtg
     7381 cagccacagc gagccggaac ccggcccgcc gttgaacagg aaggtgatcg gccggttcgg
     7441 gtcgcgcggc tccttcacat aagcgatcga gaagatactg gccttgggcg tcccgtcctc
     7501 ggccttgagg taggtctcgc ccgcggtcgc gacataacgc atccgctgcc cgccgaacgt
     7561 gccgctgtgc cgggtgacgg agatcgtcgg cggcggcacc ggcgcggccg gcgcgggcgg
     7621 cgcgggcgcg ggtgccactt ccttctgggc gggcgcaggg tcgccctggg cgcgcgaagg
     7681 cgctgcgagc gcaaggctgc ccgctgcgaa caggaggatc gagagggccg aattcatcga
     7741 gagtccctga aggtggcgtc gcccgccgcg ctatcgcacg ccttttccgt aagtcaaagc
     7801 cgcgcccggg ctttcaggcc atgccggtaa tcgggcaggt gacggtgttg ggtatctcgc
     7861 tcgtcaccgg cggctgcgga taatattggg tcatctgctg gacgctctgg gccgtggcag
     7921 tgcaggtgag cagctcgatc agcttcagcg cctcgtccag gccggaggag atgccgcctc
     7981 cggtcagccg gtcccggtcg tggatgaagc gcggatgccc gggcgccacc ttcacgttcg
     8041 gataggcctg catcaggcag gggatgaagg cccagtgggt ggtcgcctca tagccgtcga
     8101 gcagaccggc cgccgcgagc agcagcgccc cttcgcagac cgacgcgagg tagcgggccg
     8161 tctccgcctg cgcgcgaaga aagctcatat agggcccgcc ggggtcggcg atgatggcgg
     8221 ccaatgccga cggatcgccg ccgggcaccc agatcgcgtc ataggccggc gcggctgcat
     8281 cgaagctcgt cgctacctcg aatgaaaaac ccttgcggaa ggtgaccgtc cccgcgtcga
     8341 cggcgagcag ctcgacgccg atgtcggccc aggtcagcat ctcgaagacg ccggtcacgt
     8401 cgagcatatc gacctgatcg tagacaggga tcgccacggt cggtcctgaa ggttgatagg
     8461 ccatcgtccg ctcctactga atctccgcct tgaacaacgg cgcatccccc gcgaaggcgg
     8521 cgagcgccgg cgcctcgagg cgcgtctgcg cgcccgcggc cgtcccggag gcgaggatgc
     8581 gggtgcgggt gcggatctcg acatggaccg gaggcaccgc tccttccgcg ccctcgggca
     8641 ggcggaagct cgtccgcgcc tccagatgga cttggcagtt gatgcagcct tcgacgtgcc
     8701 gccggcttag caccgcctcg tggccgagcg gccgaacctc gccgtcgata ttcgcataag
     8761 cggagatgac gaaagagccc cggatcttgc tccggtcgat gccgcttaca taaacggccc
     8821 ggcgaccctc cgctgaagcg gccgctccgg ggaggagcgc cgcttcggac gcgtcgacga
     8881 aacggtcgag cgagccaaag ccgtaatcgt agccgagctg gcggatatcg gtgacgtcgt
     8941 tggttgtcat cggccgggta ccgccgcttc cgtcgtccat caagaacggg ttgagcggcg
     9001 agtccgccgt cagcggtgtg tcgggcggag tgccggccgc cggcccctgc gcggtgccgg
     9061 tgctggagtt cgcgccgggg tcggcataat cgatggtgag cggcgcgctc gtcgcgccat
     9121 gcttcttctg ccaggtccag aaggcgtaat cgatgaagca gtgatggaag aagaagatcg
     9181 ggtccagccc ggcggtgtcg ttttcgccca tgtccccgtt ggcgccgggg atcggcgaga
     9241 aatccccctg gccgggaacg tcgaaccccc caaccgcgag gtgcacggcg ttgtgcgggc
     9301 tttcgagcgg caccaccagc tgctgcggat tggccttgtt ccaggcgccg gccgaggtgg
     9361 tgttggagaa caaagtataa gtgggcgtat tgaggcagga gacgtagcgg tcgtagaccg
     9421 cggctcccac cggcttcccg ttcacaaccg ccattccatt gagccagtcg gcgacattct
     9481 gattaagcag cccgacattg gtggcgtcat tggggaactt ggcgttgtgg gcggcggtcg
     9541 ccgcctggtc cgcgggcgtg ccgaccagtc cggagagtgg atagcgcacc gtctcgtaac
     9601 cggccggctt ggaatagagt gagtcgtcac cctgcaactg atcgccgagc gcgaccggca
     9661 ggatgaagga tctcagcgga ttcgcgatga tctccccatc aagctcgaac gtcgcccagg
     9721 tcagcgccgc gggaattccg taggagcgcg aggcagggct gcactcgtcc caataaggca
     9781 gcatcacatt ctcgcagccg gctatgctct gcagcgcctt ttccaacaca tggagatagg
     9841 cgcggtgcca ggtcgggaag agcacggtgc cgtgctcgca atagccgccc caataagccg
     9901 ggtcggttgc acccggccca cggaacggct cgccatgata gccgccaatc acgaagaaag
     9961 aacgaggatc ctccggcggc agtgccttga tgcccctcca ggcgcgcatc aaattctcaa
    10021 gctcttcctt attgccattg tcgtaatcgg tttggacgtc gaacagggac cgacgaacag
    10081 cagtggttcc ggacgttggc acgataagcc ctcccctaaa tgattgagga aacgattttt
    10141 tatgcgctgg ccacaaaaat gaaataagcg agcgtaccaa agcgcgctca atagcattgc
    10201 aggacaaaga agaagagact caacaccttc gctgtgcgat tcgttcaaag catacgcttc
    10261 tcgactttat tttcaaacat agatttattt gggttggggt ttatcaaacg g
//
LOCUS       DASVRK010000266         7839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_429011, whole genome shotgun sequence.
ACCESSION   DASVRK010000266 DASVRK010000000
VERSION     DASVRK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429011"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VF589_10620"
     CDS             complement(<1..870)
                     /locus_tag="VF589_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX8258076.1"
                     /translation="MSLILVIGGYGGFGARLCRRLAGTGHDLLVAGRSAAKAEPFCAT
                     LTGAEPIRMDRLADIGPVLARRRPDLVIDAAGPFQDSGYAVPEACIDAAIPYLDLADA
                     RGFVTGIGTLDERARAAGVAVISGASSAPALTGAVARALAAGLDRVDGVDIALSAANR
                     ASGGDAVVAAILSYVGRPVRLWRGGRWTRGFGWQEMRREDFVLSDGTALRGRYVALAD
                     VPDCELLPALLPGRPAVSFRAGTELAFQMWALWLASWPVRWGWVKSLGAVTRLVLPLY
                     RMTGRIGGERSAMS"
     gene            complement(867..1271)
                     /locus_tag="VF589_10625"
     CDS             complement(867..1271)
                     /locus_tag="VF589_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HEX8258077.1"
                     /translation="MSGEDRPIVVFDGLCPLCSANARFILKHDRSGRLRLAAMQSEAG
                     AALYRRFGIDPADPDTLILVAGGRALRDSDAVIAIWRELGWPWRLAAAASAVPRGLRD
                     PLYRWIARNRYRLFGRNSACWLPDPRYADRLL"
     gene            complement(1268..1969)
                     /locus_tag="VF589_10630"
     CDS             complement(1268..1969)
                     /locus_tag="VF589_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEX8258078.1"
                     /translation="MAREQSGAKVLPFPARARPAPEPGPLGDLRFRKLVGEAAWARLP
                     EAVRARFGHHPGHGTIVYAGEVVECRMSRCGRLLAQLLRAIGGPLPLSRDTFVPAVVT
                     VTEDPASGGQFWTRLYGRGRGFPQVIHSSKRFCGPTGLEEYVGRGVGIALRVAVEDGA
                     LHFLSDHYFVALMGVRLRLPRWLSPGALKVSHIDCSHGCFAFVLALHHPLLGELVRQT
                     ALFHERTPAGAGGLA"
     gene            2144..2950
                     /locus_tag="VF589_10635"
     CDS             2144..2950
                     /locus_tag="VF589_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8258079.1"
                     /translation="MSERDYILGTHDDEVERLGLQHRVWRPRMLDAWRRAGIGPGQTV
                     IDVGAGPGWASLDLAEMVGPNGRVVAVERSRRFLDALTARAERLGLANVEAVERDLAE
                     EGLGESVADAAWCRWLLCFLADPAAAVAKIGAALKPGGVAVFHEYADYGAWQMMPPNE
                     TVDRFRSLVIQSWRDAGGDPDVGLRLPGWLEDHGFDLIELRPLIDVTHPRDFVWQWPA
                     AFMAVNARRLHSLGYATAEESERMAAALDEAGPGARMITPLVVEVIARKS"
     gene            complement(2954..3736)
                     /locus_tag="VF589_10640"
     CDS             complement(2954..3736)
                     /locus_tag="VF589_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX8258080.1"
                     /translation="MVEAAAKRDVTGLLTRAAIALAFGGVAAALVAAIGSGQGLWHFR
                     GAFAVLKWAFYAVVAGAVLALVGLVLARRARQSRLVAANAIALVVALAFILYLGAQVR
                     TARSVPAIHDVATNLDDLPQFSRLAVRPDNLEEVPAGDRAELAALDPEARWKALHREA
                     YGDLNTITVPADVPGVIRRAEALARERGWEIARVDTAAGILEATDTSTFFRFKDDVVV
                     RARPAPGGGAAVDMRSVSRIGVSDVGVNAERVREFLDDLEAA"
     gene            3823..3896
                     /locus_tag="VF589_10645"
     tRNA            3823..3896
                     /locus_tag="VF589_10645"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3855..3857,aa:Gly,seq:ccc)
     gene            complement(3972..5120)
                     /locus_tag="VF589_10650"
     CDS             complement(3972..5120)
                     /locus_tag="VF589_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX8258081.1"
                     /translation="MTGERRIDRHFGMDWLRIGAFGLLILYHVGMFFVHWDWHVKTAD
                     PLEWAAIPMLALSPWRLSLLFVVSGYATRAVLLRAKSLREFTGGRTKRLLIPLVFAMV
                     FLIAPQPWVELMWKHGYAGNYLHFWTRDYFRFGAIDGILLPTWQHLWFVLYLWGYTVI
                     AAILAPLAPRLRAQAVFDRIFGHAGALLVPLAWFLVMRGFVVQGEDETHAFVDDWPGH
                     IVYFPAFLFGLGLACSGTAWAGIRRWWKAAAAMAVLCYLPIAATEIVYPGTTPFPPSL
                     HLPYFSVRALQGAAAVIALIGLADRFWNRDGPGRKTLTEAVFPFYIIHQTIIVLVGWY
                     LLRLRLPPSGEFVILVAATALGCWLFFEAARRFRLLRPMVGLKATAKG"
     gene            5403..6311
                     /locus_tag="VF589_10655"
     CDS             5403..6311
                     /locus_tag="VF589_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8258082.1"
                     /translation="MSQTEPDAAPAAPAAAAPAAASTDADGLMKWPALLDRPRPAASA
                     TIRYGADPLQIVDLWLPEGAGPHPTVLMVHGGCWTTEIADRTIMNWIAEDLRKRGIAV
                     WNIDYRGVDRPGGGYPGTFLDAAAAADALRTHAARYKLDLSRLVATGHSAGGHLALWL
                     AARPRIRPDSKLHSVNPLPIRAVVSLGGLPDLEEAARPPGSGCGTEVIDKLVGPPTMS
                     SRDVYADTSVPRLGPLGVRQVLVNGMQDRIIPVAYAEGYAAPMRAKGDTVEVRMIDRT
                     GHVELIAPEREAWAAAVAELEKALGR"
     gene            6505..7734
                     /gene="kynU"
                     /locus_tag="VF589_10660"
     CDS             6505..7734
                     /gene="kynU"
                     /locus_tag="VF589_10660"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX8258083.1"
                     /translation="MTRDEARALDAADALRPFRERFFLPEGLVYLDGNSLGVLPKAAA
                     QRQREVVEREWGEALIRSWNEHDWIGAPQRIGAKIAALIGAAPHEVIVADSVSVNLFK
                     LITAAAALAPERPVLLSEAGNFHTDLHVASGAAELMPGLDLEVVERGAIDARLGPDVG
                     LLLLTHVHYKAGFRFHMAEVTARAKAAGATTLWDLSHSAGAVPLRLNADGAELAVGCG
                     YKYLNGGPGAPAFLYVAEHLQERLVPKLRGWMGHAAPFAFTDDYVPAPGIARFLAGTP
                     PMLSLLALESGVDSFQGADMNAVWAKSVAMFDLFAALMEERCGGHGLECISPPDPEQR
                     GSHISYRHEHAFEICQALIEAQVIGDFRAPDVVRFGLTPLYLGFEDVWEAIDRLARIM
                     ASESWREPRFAVRNKVT"
BASE COUNT         1250 a         2785 c         2728 g         1076 t
ORIGIN      
        1 gctcatcgcc gagcgctcgc cgccgatccg tccagtcatc cggtagagcg ggagcaccaa
       61 gcgcgtcacc gcgccgagcg actttaccca gccccaccgc accggccagc tcgccagcca
      121 cagcgcccac atctggaacg cgagctcggt gccggcgcga aagctgacgg cggggcggcc
      181 cggcagcagc gcgggtagca gctcgcaatc cggcacgtcc gccagcgcga cgtagcggcc
      241 gcgcagggcg gtgccgtccg acaggacgaa atcctcgcgg cgcatctcct gccagccgaa
      301 gccgcgggtc cagcgccccc cgcgccagag gcgcaccggc cggccgacat agctgaggat
      361 cgccgccacc acggcgtcgc cgccgctcgc ccgattggcg gcgctgagcg cgatgtcgac
      421 gccgtcgacc cggtcgagac cggcggcgag cgcgcgggcg acggcgccgg tcagcgcggg
      481 agcgctcgag gctcccgaga tcaccgccac ccccgccgcc cgcgcgcgtt cgtcgagggt
      541 gccgatgccg gtgacgaagc cgcgcgcatc ggccaggtcg agatagggga tggcggcgtc
      601 gatgcaggcc tcgggcaccg catagccgct gtcctggaac ggcccggcgg catcgatgac
      661 cagatcgggg cggcggcgcg ccagcacggg cccgatgtcg gccagcctgt ccatccggat
      721 cggttcggcg ccggtcagcg tggcgcagaa gggctcggcc ttggcggcgc tgcgccccgc
      781 caccagcaga tcgtggccgg tgccggccag ccggcggcac agcctcgccc cgaagccgcc
      841 atagccgccg atgacgagga tcaggctcac agcaagcggt cggcgtagcg cgggtcgggg
      901 agccagcagg ccgaattgcg gccgaacagg cggtagcggt tgcgcgcgat ccagcggtag
      961 agcgggtccc gcagcccgcg cggcacggcg ctcgccgcgg ccgccagccg ccacggccag
     1021 ccaagctcgc gccagattgc gatcacggcg tcgctgtcgc gcaaggcgcg gccgccggcg
     1081 acgaggatca aggtgtccgg gtcggcggga tcgatgccga agcggcggta gagcgccgcg
     1141 cccgcctcgc tttgcatcgc cgcgagccgc agccggccgc tccggtcgtg cttcaggatg
     1201 aaccgggcgt tggccgagca gagcgggcac aggccgtcga acacgacgat ggggcggtcc
     1261 tcgccgctca tgccaggcct cccgcgccgg cgggggtgcg ctcgtggaac aaggcggtct
     1321 gccggacgag ctcgccgagc agcgggtgat ggagcgcgag cacgaaggcg aagcagccat
     1381 ggctgcagtc gatatggctg accttgagcg cgccgggcga gagccagcgc ggcaggcgca
     1441 gcctcacccc catcagcgcg acgaaataat gatcgctgag gaagtggagc gccccgtcct
     1501 cgaccgcgac ccggagcgcg atgccgacgc cgcggccgac atattcttcc agcccggtcg
     1561 gcccgcaaaa gcgtttcgag ctgtggatga cctgcgggaa gccgcggcct cggccgtaga
     1621 ggcgggtcca gaattgcccg ccgctcgccg gatcctcggt gacggtgacc acggccggta
     1681 cgaacgtgtc gcgcgacagc ggcagcggac cgccgatcgc ccgcagcagc tgggcgagca
     1741 gccggccgca ccggctcatc cggcattcca ccacctcgcc ggcatagacg atggtgccat
     1801 ggccgggatg atggccgaac cgcgcccgca ccgcctccgg aagccgcgcc caggccgctt
     1861 cgccgacaag cttgcgaaag cgcaggtcgc cgagcggtcc aggctccgga gcgggccggg
     1921 cgcgcgccgg gaaaggcagg accttcgcgc cggactgctc ccgtgccatc acccttcctc
     1981 tcattccgtc ccggccgaat cgccggagcc agtctggctg ttatttctgt tatgacagaa
     2041 atatctgata actcagcatg tatgtcaagg ctccttttgg ccgcaagaag cggataggcg
     2101 tccaatgccg ggatgctcat gagcgtccgc gaaggagacg gcgatgagcg aacgcgacta
     2161 tattctcggc acccatgacg acgaggtcga gcggctcggg ctccagcatc gcgtctggcg
     2221 cccgcgcatg ctcgacgcct ggcgccgcgc cgggattggc cccggtcaga cggttatcga
     2281 cgtcggcgcc ggccccggct gggcgtcgct ggacctggcc gaaatggtcg ggcccaacgg
     2341 aagggtggtc gcggtcgaac gctcgcgccg ctttctcgat gcgcttaccg cccgcgccga
     2401 acggctgggg ctcgccaatg tcgaagccgt cgagcgcgat ctggccgagg agggcctcgg
     2461 cgaaagcgtg gccgacgccg cctggtgccg gtggctgctc tgcttcctcg ccgatccggc
     2521 cgccgcggtc gccaagatcg gcgccgcgct gaagccgggc ggcgtcgccg tcttccacga
     2581 atatgccgat tacggcgcct ggcagatgat gccacccaat gagaccgtcg accgcttccg
     2641 cagcctcgtc atccagagct ggcgcgacgc cggcggcgat cccgacgtcg gcctgcggct
     2701 tcccggctgg ctcgaggatc acggcttcga cctgatcgag ctccgccccc tgatcgacgt
     2761 cacccatcct cgcgatttcg tctggcaatg gccggcggcg ttcatggcgg tcaacgcccg
     2821 ccgcctccac tcgctcggct atgcgaccgc cgaggaaagc gagcgcatgg ccgccgcgct
     2881 cgacgaggcc ggccccggcg cgcggatgat cacgccgctc gtcgtggaag tgatcgcccg
     2941 caaaagctga gcctcacgcg gcctcgaggt cgtcgaggaa ttcccgcacc cgctcggcat
     3001 tgacgccgac gtcgctgacg ccgatccggc tgaccgagcg catgtcgacc gcggcgccgc
     3061 cgcccggcgc cggccgcgcc cgcaccacca catcgtcctt gaagcggaag aaggtgctgg
     3121 tgtcggtcgc ctcgagaatg ccggcggcgg tgtcgacccg cgctatctcc cagccgcgct
     3181 cgcgcgccag cgcctcggcg cggcggatga cccccggcac atcggcgggg acggtgatcg
     3241 tgttgaggtc gccataggcc tcgcggtgga gcgccttcca gcgcgcctcc ggatcgagcg
     3301 cggcgagctc cgcccggtcg ccggcgggca cctcctcgag attgtccggc cgaaccgcca
     3361 gccggctgaa ctgcggcagg tcgtcgagat tggtggcgac gtcgtggatc gccggcacgc
     3421 tccgcgcggt ccggacctgc gcacccagat aaaggatgaa ggcgagcgcg acgaccagcg
     3481 cgatggcgtt ggccgcgacc agccgcgatt gccgcgcccg gcgggcgagc acgagcccga
     3541 ccagcgccag cacggctccg gcgacgactg cgtaaaacgc ccatttcagc accgcaaagg
     3601 cgccgcggaa atgccacagc ccctgcccgc tgccgatcgc cgcgaccagg gccgcggcca
     3661 cgcccccaaa agcgagcgcg atcgccgccc gcgtcaacag ccccgtcacg tcccgtttcg
     3721 ccgccgcttc aaccatcgct ccgcctccct cgccgcctca tgctgcgcta cccaagccct
     3781 ctcgacaagc ccggcgccgc gccatagata atcacgctgc gggcgggtgt agctcaatgg
     3841 cagagcagaa gcttcccaag cttacgacga gggttcgatt cccttcaccc gctccagcgt
     3901 taacatagct gctcgatcga ccggtcttgc ggtaaccagt gcgacccccg cagcgagggg
     3961 gccacggccg atcagccttt tgccgtcgcc ttgaggccca ccatcggccg cagcagcctg
     4021 aagcgccggg cagcctcgaa gaacagccag cagcccagcg cggtagccgc gacgaggatc
     4081 acgaactcgc cgctcggcgg gagcctgagg cgaagcagat accaaccgac cagcacgatg
     4141 atcgtctggt gaataatgta gaagggaaag acggcctccg tcagcgtctt ccgcccaggg
     4201 ccgtcgcggt tccagaagcg gtccgccagt ccgatcaggg cgatcactgc cgccgcgccc
     4261 tggagcgcgc gcaccgagaa ataagggaga tgcagggagg gcggaaaagg ggtcgttccc
     4321 ggatagacga tttcagtcgc ggcaatcggc aggtaacaca ggaccgccat ggccgcggca
     4381 gccttccacc agcggcgtat tccagcccag gcggttcccg agcaggcgag acccaggccg
     4441 aacaggaagg cggggaaata cacgatgtgg cccggccaat cgtcgacgaa tgcgtgagtc
     4501 tcatcctccc cttgaacaac gaagccgcgc atcaccagga accatgcaag cggcaccagg
     4561 agcgctccgg catgtccgaa gatacggtcg aaaacggctt gcgcgcgaag cctgggggca
     4621 agcggcgcca ggatcgcggc gatgacagtg tagccccaca ggtagagcac gaaccaaagg
     4681 tgctgccagg tgggaaggag gatgccgtcg atggctccga accgaaaata gtcccgggtc
     4741 cagaagtgaa gatagtttcc ggcatagcca tgcttccaca tcagctcgac ccacggctgg
     4801 ggcgcaatca ggaagaccat tgcgaagacc aggggtatga gcaggcgctt ggtgcggccg
     4861 cccgtgaatt cgcggaggct tttggcccgc aacagcaccg cgcgcgtggc atagccggac
     4921 accacgaaca gcagtgacaa ccgccagggg ctcagcgcca acatcggaat tgcggcccac
     4981 tccagtgggt ccgccgtctt cacgtgccag tcccaatgca cgaagaacat gcccacgtgg
     5041 taaaggatca acagcccgaa cgcgccgatc cgcagccagt ccatgccgaa atggcgatcg
     5101 atcctgcgct cccccgtcat gagtcgcgcc taatcgctga gatcgatcct gtcgatgcag
     5161 cctgccaggc gcgtgcggca tcaatggctg gcggcggcga tcaggctggc ctcgtgcgcg
     5221 acggcggcag ctggcggatg cggtcggtgc gcgcgcaccc gctgcccgcg cgctgagctt
     5281 gcgcgcggcc cggcgcggtc tatggcgggc gccgaacgga gtccgccatg cctcgccccg
     5341 cccgcgccct tgcctcccct gcccttgcct ctgccgccct gctggcgcta gccggctgtg
     5401 cgatgagcca gaccgaaccc gacgccgcgc ccgccgcgcc cgccgccgcc gcgcccgccg
     5461 ccgcttcgac cgacgcggac gggctgatga aatggcctgc tttgctcgat cggccgcgcc
     5521 cggccgctag cgcgacgatc cgctatggcg cggacccgct gcagatcgtc gatctgtggc
     5581 tgcccgaagg cgcggggccg cacccgacgg tgctgatggt ccatggcggc tgctggacga
     5641 ccgagatcgc cgaccggacg atcatgaact ggatcgccga ggacctccgg aagcgcggca
     5701 tcgcggtctg gaacatcgac tatcgcgggg tcgaccggcc cggcggcggc tatcccggca
     5761 ccttcctcga cgcggccgcg gcggcggatg cgctgcgcac gcacgccgcc cgctacaagc
     5821 tcgatctgtc gcggctggtc gcgaccggcc attcggcggg ggggcacctc gctttgtggc
     5881 tggcggcgcg gccgcggatc cggccggaca gcaagcttca cagcgtcaac ccgctgccga
     5941 tccgggccgt ggtcagcctc ggcggcctgc ccgacctcga ggaagcggcg cgcccgccgg
     6001 gcagcggctg cggcaccgag gtgatcgaca agcttgtcgg cccgccgacc atgagcagcc
     6061 gcgacgtcta tgccgacacg tcggtgccgc ggctcgggcc gctcggggtg cgccaggtgc
     6121 tggtcaacgg catgcaggac cggatcatcc cggtcgccta tgccgagggc tatgccgcgc
     6181 cgatgcgggc caagggcgac accgtcgaag tccggatgat cgaccgcacc ggccatgtcg
     6241 agctgatcgc gcccgagagg gaagcctggg cggcggcggt ggcggagctg gaaaaggcgc
     6301 tggggcgatg atcctccccg gaccggggag ggggaccgcc gggcgcagcc cggtggtgga
     6361 gggggcccgg ggacccgtcg tgcgtgcgga gtggcccctc caccatgagc aggtgcgcag
     6421 cgccccgcgc tgcgcagcca tgtccggggg acatggcgac ctgctcattg cggctggtcc
     6481 ccctccccgt tccggggagg atttatgacc cgcgacgagg cccgggcgct ggatgcggcg
     6541 gacgcgctgc ggccgtttcg ggagcgcttt ttcctgcccg aggggctggt ctatctcgac
     6601 ggcaattcgc tcggcgtgtt gccgaaggcc gccgcccagc gacagcggga ggtggtggag
     6661 cgggaatggg gcgaggcgct gatccgcagc tggaacgagc atgactggat cggcgcgccg
     6721 cagcgtatcg gggccaagat cgcggccctg atcggggccg cgccgcacga ggtgatcgtc
     6781 gccgattcgg tgtcggtgaa cctgttcaag ctgatcaccg ccgcggccgc gctggcgcca
     6841 gagcggccgg tgttgctgtc cgaagccggc aatttccaca ccgacctcca cgtcgccagc
     6901 ggcgcggccg agctgatgcc cgggctagat ctcgaggtgg tcgagcgcgg cgcgatcgac
     6961 gcgcggctcg gacccgatgt cggcctgctc ctcctcaccc atgttcatta caaggccggc
     7021 ttccgcttcc atatggccga ggtcaccgcc cgcgccaagg cggcgggggc gacgaccttg
     7081 tgggacctca gccacagcgc cggggcggtg ccgctgcggc tcaacgccga cggcgccgag
     7141 ctggcggtcg gctgcggcta taaatatctg aacggcggcc ccggagcgcc ggctttcctc
     7201 tatgtcgccg agcatctgca ggagcggctg gtcccgaagc tcaggggctg gatgggccat
     7261 gccgcgccgt tcgccttcac cgacgattat gtgcccgcgc ccggcatcgc ccgcttcctc
     7321 gcgggcacgc cgccgatgct gagcctgctc gcgcttgaaa gcggcgtcga cagctttcaa
     7381 ggcgccgaca tgaacgcggt atgggccaaa tcggtggcca tgttcgacct gttcgccgct
     7441 ttgatggagg aacgctgcgg cggccacggc ctcgaatgca tctcgccgcc ggacccggag
     7501 cagcgcggca gccatatctc ttaccggcac gagcatgcgt tcgaaatctg ccaggcgctg
     7561 atcgaggcgc aggtgatcgg cgatttccgc gcgcccgacg tggtccgctt cggcctgacc
     7621 ccgctttatc tcggcttcga ggacgtctgg gaggcgatcg atcggctcgc ccgcatcatg
     7681 gcctcggaga gctggcgcga gccccgcttc gccgtccgca acaaggtgac ctgatcgcgg
     7741 aatcagttca cgatggccgg gtggaggggt caccgtgatt gcgcctcccc acctgatcca
     7801 ctatagctcc ggcaaccgcc tcggccccgg ggggggggg
//
LOCUS       DASVRK010000267        20405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_429610, whole genome shotgun sequence.
ACCESSION   DASVRK010000267 DASVRK010000000
VERSION     DASVRK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 20405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20405
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429610"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VF589_10665"
     CDS             <1..174
                     /locus_tag="VF589_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEX8258084.1"
                     /translation="VNEAVWRTTTTDFWVGFKLWGALPATFVFALANVPMLMRHGMQL
                     DKPKEEPPVPPTQ"
     gene            183..1115
                     /locus_tag="VF589_10670"
     CDS             183..1115
                     /locus_tag="VF589_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8258085.1"
                     /translation="MQPIISISNLGKRYASGLEALKNVDLEIRRGEIFALLGPNGAGK
                     TTLISIVCGIVRATSGEVKADGHDIVRDYRAARSMIGLVPQELNTDAFESVWATVTFS
                     RGLFGRAPDPAYIEKLLRDLSLWDKRKTVIKELSGGMKRRVMIAKALSHEPDILFLDE
                     PTAGVDVELRRDMWQLVRDLRTKGVTIILTTHYIEEAEEMADRVGVISKGELILVEEK
                     TALMQKLGKKQLTLNLSEPMATIPAELAEWKLALKADGNELEYVFDANEERTGIPSLL
                     RRMSDLGIGFKDLHTRQSSLEDIFVSLVTDRAAA"
     gene            1112..1891
                     /locus_tag="VF589_10675"
     CDS             1112..1891
                     /locus_tag="VF589_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8258086.1"
                     /translation="MSAAPAFNARGVWAIYRFEMHRFRRTLWTGLAVPVITTSLYFIV
                     FGSAIGSRMTEIGGVPYGAFIVPGLMMLSLFTESIFNASFGIHMPRFTGTIYELLSAP
                     LSAFETVLGYVGAAATKSTIVALVILATAHLFVDLTIAHPVLMLAYLLLVAATFCLFG
                     FIVGIWAKGFEQLQVIPLLVVTPLTFLGGSFYSIDMLPEPWRTITLFNPIVYLINGFR
                     WTFFGAADVPVAWSLLATLGFFFACLAVVAWIFKTGYRLKT"
     gene            complement(2042..2656)
                     /locus_tag="VF589_10680"
     CDS             complement(2042..2656)
                     /locus_tag="VF589_10680"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX8258087.1"
                     /translation="MPFTLPPLPYGFDALEPTIDAATMQLHHDKHHQAYVDKLNEGIA
                     EDPALEGRSLEDILASISSQLGKVRNNGGGHWNHSFFWETMGPGGTGRPSGRLAEAVD
                     AEFGSFDAFKKKFGDAGVGQFGSGWVWLLVDGSGSLEVTATPNQDNPLMDIVQVQGTP
                     ILGNDVWEHAYYLKYNNRRADYLAAWWDVVNWDKVSERYEAAAR"
     gene            complement(2772..3332)
                     /locus_tag="VF589_10685"
     CDS             complement(2772..3332)
                     /locus_tag="VF589_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258088.1"
                     /translation="MNKFVSALAFSVAAAAIAGPALAAVFVVGSSSARICYEAAESGV
                     VTNQAAVFCDKALTEDVLSPRDIVATHVNRGIVRFHKGQYDAAIRDFDVAIARDGNQA
                     EAYLNKGVVMLRKPGGVGEAVPLFTTAIEMRTKKPALAYLGRGMAYELSGNVNAAYAD
                     YKQANLLAPKWKAPAAELGRFQVVQN"
     gene            complement(3459..4544)
                     /gene="pspF"
                     /locus_tag="VF589_10690"
     CDS             complement(3459..4544)
                     /gene="pspF"
                     /locus_tag="VF589_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HEX8258089.1"
                     /translation="MDRTTQMVGQSSSFLDAVERASRAAPLDRPVLVIGERGTGKELI
                     AERLHHLSSRWDGPLVTLNCAALPETLIEAELFGHESGAFTGATRARQGRFEEADGGT
                     LFLDELATLSSPAQDRLLRAVEYGEVTRIGASKPMRVDVRIVAATNEHLPRLVDEGRF
                     RADLLDRLSFEVVTLPPLRHRSSDIPVLADHFARRMALELDWPRFPGFSTAAAARLLN
                     YDWPGNVRELRNVVERAVYRWADPENPIDAIQFDPFESPWRPLPERQHATLEILPPRL
                     EMASTGAVSPYDPAGCADFRLAVAEYEKAILANALEKCRWNQRAAAAALQLSYDQLRH
                     AMKRHQMSTAPGGPGQEEDRRSEPVEA"
     gene            4727..4873
                     /locus_tag="VF589_10695"
     CDS             4727..4873
                     /locus_tag="VF589_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258090.1"
                     /translation="MFNSTKTELQRIAAAALGALILTTACVGAAVGPARAVETAPTQV
                     SAIA"
     gene            4923..5603
                     /gene="pspA"
                     /locus_tag="VF589_10700"
     CDS             4923..5603
                     /gene="pspA"
                     /locus_tag="VF589_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEX8258091.1"
                     /translation="MGIFSRTRDIVAANFTDLLDKAEDPAKMIRMIIMEMEETLVEVR
                     ASAARTIADQKEMRRQIAKLDRLQESWTEKAELALSKDREDLAKAALVEKQKAGDMAD
                     SLRAEIAVLDDGLRASEADIAKLQSKLREARTRQNAIVSRLESAQNRSRLREMYAGPK
                     VEDAFSRFEVLERRADFAEGRADALALGAPPKTLEEEIAELRSSEKVEAELAALKAKR
                     AGATGGEA"
     gene            5608..5904
                     /gene="pspB"
                     /locus_tag="VF589_10705"
     CDS             5608..5904
                     /gene="pspB"
                     /locus_tag="VF589_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HEX8258092.1"
                     /translation="MEEAIVGIIAGCIAIVTLFVGLPWLIFHYVTKWKSQATLTAEDE
                     NLLDDLHDLARRLDDRLATVERIMTAENPAWRQLAADPAGIGIDDLSSVRRLSK"
     gene            5901..6287
                     /gene="pspC"
                     /locus_tag="VF589_10710"
     CDS             5901..6287
                     /gene="pspC"
                     /locus_tag="VF589_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HEX8258093.1"
                     /translation="MNAPRRTKFYLDRRNRKWKGVCAGIADYTGVDVVVIRIGLVILT
                     FAGGFPWTLIAYWTTAWMAPHKPRELSDESPEEAKFWQGVRASPARTVRDVRSRFRDI
                     DRRLADVETYVTSSNSRLANEIESLR"
     gene            6349..6651
                     /locus_tag="VF589_10715"
     CDS             6349..6651
                     /locus_tag="VF589_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258094.1"
                     /translation="MAQLFPFLAVIVSVAIGGWVLTTWLRIKNGYPLDGMWGQAVYPK
                     KNEEAMERIKLLSSENAQLRAELGSIKDRLANVERIVTDDSHRLTQEIESLRVPRN"
     gene            6732..6965
                     /locus_tag="VF589_10720"
     CDS             6732..6965
                     /locus_tag="VF589_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258095.1"
                     /translation="MLSSHIMVVLIVTIAVVGSILRARYKAGAHRSIADDPEAQRLRD
                     EVKQLKDRLAVLEQITIEKENSLERQIEALRDR"
     gene            7035..7442
                     /locus_tag="VF589_10725"
     CDS             7035..7442
                     /locus_tag="VF589_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX8258096.1"
                     /translation="MTALGDIYEDYELLEADDRYRLLIDLGRALEPMPDALKTDATLV
                     RGCSASVWVYPTQLADGRLHFLADSNAAITKGIIALVLAAVQDRSAAEILGDDIGAAL
                     APFDLGNQLSSNRTQGIPNMIALIRATAERLDG"
     gene            7487..7906
                     /locus_tag="VF589_10730"
     CDS             7487..7906
                     /locus_tag="VF589_10730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8258097.1"
                     /translation="MAFVEKLGHGGALGIRYHGHAAGWVELTLDYDEKLVGMVESGII
                     ASGPIISLMDMATSLAVWARLGRFRNQATLDMRIDYMRPAVPGRAIVGRGECYAATRS
                     VAFVRGLAHDGYAADPVAHVTATFMFTDQPAPEAGGA"
     gene            complement(7903..8436)
                     /locus_tag="VF589_10735"
     CDS             complement(7903..8436)
                     /locus_tag="VF589_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8258098.1"
                     /translation="MTRQTRSNDWGFPRWRGYGSDRAAQQVRLCDRHGCTEPGDRPAP
                     KAPNKPDRWYFCERHAAEYNKNWNYFQGLSAEEAAAREADERRDHGGYKETGHYGWGG
                     PGDGSRSRDEMRALDVLELEADADFDAIKAAYRRLAKANHPDVAPSAEAASRFQAIQA
                     AYEVLRKSEERRGSLSS"
     gene            complement(8560..9984)
                     /gene="ahcY"
                     /locus_tag="VF589_10740"
     CDS             complement(8560..9984)
                     /gene="ahcY"
                     /locus_tag="VF589_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8258099.1"
                     /translation="MATQPQTPFNDYVVRDLDLAAFGRKEIEIAETEMPGLMALRSEY
                     GASQPLKGARVTGSLHMTIQTAVLIETLIALGADIRWASCNIFSTQDHAAAAIAASGV
                     PVFAIKGETLEEYWDYIDRIFAWGPGGTWTETTNMILDDGGDATMFALWGARIEAGEE
                     FPAPQNEEEEVFQQVVRRRIEQSPGFLTRTVEAIKGVSEETTTGVHRLYELSKQGKLP
                     FPAINVNDSVTKSKFDNLYGCKESLVDAIRRATDVMLAGKVATVAGFGDVGKGSAQSL
                     RNGGARVLVTEVDPICALQAAMEGFEVITMEEAVTRADIFVTATGNADVITVDHMRAM
                     KNMAIVCNIGHFDSEIQITALQNMKWTEIKPQVDEVEFPDGKKIIVLSKGRLVNLGNA
                     TGHPSFVMSSSFTNQVLAQIELWTGGDKYQNDVYVLPKHLDEKVAALHLDKLGVKLTK
                     LTDKQASYIGVPVEGPFKPEHYRY"
     gene            complement(10230..10793)
                     /locus_tag="VF589_10745"
     CDS             complement(10230..10793)
                     /locus_tag="VF589_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX8258100.1"
                     /translation="MQEEPRFLTGQFLLAMPGIGDPRFEKAVIAMCVHDEGGALGIGL
                     GRIIPRLGFHDLLKQLDIPVGTAPDSAIHLGGPVEPQRGFILHSLDWGGDGSVQVSNR
                     WTLTATLDILRAIAEGRGPTRWVSALGYAGWGAGQLEQEMCGNGWFTTPGSDELLYDC
                     DVNSRWTTAFQSAGIDTRLLTADFGTA"
     gene            10873..11355
                     /locus_tag="VF589_10750"
     CDS             10873..11355
                     /locus_tag="VF589_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8258101.1"
                     /translation="MSISVGDRIPPIPFIKATPEGPAPVESENYFRGRKVALFSVPGP
                     FTPTCSARHLPGFIDKADALKAAGIDEIACTAVSDAFVMQAWAEQSGAAGKVTMLADG
                     NGAFVKAVGLEMDASKFGMGQRGQRFSMLIDDGVVSQLNVEEPGAFNVSSADYLLERL
                     "
     gene            11471..11800
                     /locus_tag="VF589_10755"
     CDS             11471..11800
                     /locus_tag="VF589_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258102.1"
                     /translation="MAEGRTPHEAGDSGRSDSSDGSDAEFVAGGAQWHPMKAAALVGG
                     AIAAGAGAYLGTRALARRNASSDGRKLNSVMATAITACNLAQKQPATEAEPAVAPEPT
                     VPREPRV"
     gene            complement(11801..12340)
                     /locus_tag="VF589_10760"
     CDS             complement(11801..12340)
                     /locus_tag="VF589_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258103.1"
                     /translation="MKAAATAALLAAAAAPAGAALPTARFNPAAPENRQLLPAFSYAT
                     VEPVLAQIGARYRRAGASPEKPVLHVVLPNSRTAILSFDMCNPGGTACKSLSIHSEWT
                     RIANSPPERTERAIDGFNRSFAFAKAFVAPGGKPWLQRYLTADYGFVRGNLAVNLLVF
                     AEQAESFAVKVLRPLEARR"
     gene            complement(12396..14285)
                     /locus_tag="VF589_10765"
     CDS             complement(12396..14285)
                     /locus_tag="VF589_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX8258104.1"
                     /translation="MKATVSALAVATLLAVGGASFAQSSNQGAQQRRAPAAAKAGPAT
                     AAEADAFVARAEKELAEFSNISSRAQWVNNTYLNDDSDAVAAHFGTIGTEMSVRLANE
                     AARYANVRGLSYDTKRKLDILRGGIVLPAPTTPGAAAELNEIATRLNSTYGKGKGTLN
                     GKAMSGSDIEEAMGTNRNPAELQEMWTSWHSNVGTPMRKDYQRLVQISNEGAKELGYK
                     DVGAMWRSGYDMPADEFAALTDKLWGQVKPLYDQLHCYTRGKLNEKYGNEVQPAAGPI
                     RADLLGNMWAQEWGNIYDVVAPKGAGDIGYDVTELLVARKYDPVKIVKTGEGFFSSLG
                     FAPLPATFWERSMITKPADREVVCHASAWNVDNVEDLRIKMCTKVNSDDFVTVHHELG
                     HNYYQRAYNKQPFLYLNGANDGFHEAIGDTVALSITPEYLVQVGLLDKSKVPSADKDI
                     GLLLRQAMDKVAFLPFGLMVDKWRWGVFGGSIPASGYNKAWNDLRLEYQGIVPPVART
                     EAQFDPGAKYHIPGNTPYARYFLARILQFQFYKAACDQAGWKGPLHRCSFYGNKEVGR
                     RLNAMLEMGASKPWPDALQAFTGSREMSGEPLLEYFRPLSAWLEQQNRGRRCGWAAGT
                     AATAS"
     gene            complement(14347..15114)
                     /locus_tag="VF589_10770"
     CDS             complement(14347..15114)
                     /locus_tag="VF589_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8258105.1"
                     /translation="MATSPADPRRHSFGSFDGVEIAWAELGEGRPVILIHGLFSNADT
                     NWIRYGHAAAIAARDFRVIMPDLRAHGRSATPHDETAYPPDVLVRDNLALIAHLGLTG
                     YDLGGYSLGARTAVRMVVHGGAPDRLVVSGMGLQGLLGTAGRADHFRRILTGLGSFER
                     GSPEWMAEAFLKTTRGDPQALLPLLGSFVDTGADELARLAMPTLVVAGAEDRDNGSSE
                     ELAASLADGRYVEIPGNHMSAVTKPELGGAIADFLAR"
     gene            complement(15095..15538)
                     /locus_tag="VF589_10775"
     CDS             complement(15095..15538)
                     /locus_tag="VF589_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8258106.1"
                     /translation="MKEVSGGCACGRVRYSARIENHDAYLCHCRMCQRASGNVSLAMK
                     TLPQSDVTWEREPEYYQSSAIARRGFCSACGTSLTFQYVEGTDKLDLIVGTFDDPSPF
                     VPKHHFGAESMHRAWLNTSGLPEMRSEDYQPLVNRWMDKVGNLPG"
     gene            complement(15539..15946)
                     /locus_tag="VF589_10780"
     CDS             complement(15539..15946)
                     /locus_tag="VF589_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8258107.1"
                     /translation="MPLVVDASVAVKFLVREPGNDQARRLLSIPDPLIAPDWLLVEAA
                     NTFWKKVKRSELLADHAVQHLQDLPEFFELLYPASDLIEDAFRWSIGLRHPVYDCIYL
                     ALAEREGCKLVTADEKFAAALARAGLSEKVEAF"
     gene            complement(15930..16229)
                     /locus_tag="VF589_10785"
     CDS             complement(15930..16229)
                     /locus_tag="VF589_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258108.1"
                     /translation="MEQVVIRRLKPGTLAPYRKAAKAKGRSLEAELREVIETHCPKRT
                     KNSADLLRISDDALALTPPDGLGEASDSTLLIRWDRDTGGGRWIDDGWDDDAAGR"
     gene            complement(16261..17289)
                     /locus_tag="VF589_10790"
     CDS             complement(16261..17289)
                     /locus_tag="VF589_10790"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX8258109.1"
                     /translation="MGYRVVVVGATGNVGREILNILAEREFPADEVAAVASPRSSGTE
                     IDFGDSGRLLKVRNIDHFDFAGWDMALFAAGSEVSRLHAPRAAAAGCTVIDNSSLYRM
                     DPDVPLIVPEVNAHAIAGYTKRNIIANPNCSTAQMVVALKPLHDFAKIKRVVVATYQS
                     VSGAGKAGMDELFEQSRNIFVGDSNEPHKFTKQIAFNVIPHIDDFLDDGSTKEEWKMV
                     VETKKILDPKIKVTATCVRVPGFVGHSEAINIEFENEISAKDAQRILRESPGIMLVDK
                     REDGGYVTPVEAVGEYATYVSRVREDSTVDNGLSLWCVSDNLRKGAALNAVQIAELLG
                     RKHLRKAA"
     gene            complement(17471..18379)
                     /locus_tag="VF589_10795"
     CDS             complement(17471..18379)
                     /locus_tag="VF589_10795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8258110.1"
                     /translation="MTAIVALPLRIGARTIWRVKRRLIQVGLPLEQGMSGTAPALPPL
                     GRGADGYMIYALPAALQDRVQVSHPELKCFVRQRYQRSYAALDIAFDDYLAGFSSKTR
                     STLKRKLRKFADASGGRLDLRRYSAPDEVEAFHRHARAVSAKTYQEKLLDAGLPSGEQ
                     ALADMRERARRGAMRGWVLFLGGEPVSYLYTPAEGATLIYAYLGYDPAHAALSPGTML
                     QLEAMRGLMEEGGFRLFDFTEGDGQHKRQFATGALDCVDLLLVRRTPANLIVGHTLMG
                     FDAAVALAKRAARASGLERLARSVLR"
     gene            complement(18376..19326)
                     /locus_tag="VF589_10800"
     CDS             complement(18376..19326)
                     /locus_tag="VF589_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX8258111.1"
                     /translation="MVAPVLLTVDTELTWRHYARGASWQENFACAYEAAGAGVPYQLD
                     LLRAHGLKACFFVDPMPALVYGLEPVRRMVAPILEAGQEVQLHLHSFWHCLANGGAEQ
                     GKFELTGFNPLDQRDLIATARDLLVEAGAPPPVAFRSGSYAADEITLDALASLGIRYD
                     SSHNGSHHPWPSALPLDPALIAPVEHRGVVEVPVGQIEDGDGKLRHLQVTALSFSEFR
                     AALLHAADHGHPLTTIVSHSFELATRDGLRPNPVLKLRFERLCAFLADERAQLPTAHF
                     TDLGGLPLGRAAAPLPSQPVRRARRMAEQLWANAVYERAL"
     gene            19470..20075
                     /locus_tag="VF589_10805"
     CDS             19470..20075
                     /locus_tag="VF589_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258112.1"
                     /translation="MKILLAAAATIVLLACSDQQSPAAKAAEGAAPKVEAKVATAAAA
                     YEPYLEKRYPASGKAVAAAEVAGMIERDGPQKAVAALWGEGADNGWEAVMRGIAKGDP
                     AWLALAPAISKGADAGASTGLAMASQDALTTNAAGALRLMSQLGDGAAGCTYESYEDP
                     PEQSRAFYAAATAGVEAVTDPALQTTRDACLAQLRSGAAQS"
     gene            complement(20118..>20405)
                     /locus_tag="VF589_10810"
     CDS             complement(20118..>20405)
                     /locus_tag="VF589_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8258113.1"
                     /translation="LCRWTLMAHDRIEGDEIKVTHDEIAVMLGVRRASVTDALHILEG
                     EGLIRSRRGRVTIRNRDGLRRLAGETYGYAEAEYSRLIAPFPRHGAGSQGS"
BASE COUNT         3438 a         6602 c         6949 g         3416 t
ORIGIN      
        1 gtgaacgagg cggtgtggcg gaccacgacc accgacttct gggtgggttt caagctttgg
       61 ggggcgttgc cggcgacctt cgtgttcgcg ctggccaacg tgccgatgct gatgcgccac
      121 ggcatgcagc tcgacaagcc gaaggaggaa ccgccggttc cgcctacgca atagggaaga
      181 tgatgcagcc gatcatatcc atctccaacc tcggcaagag atatgcctcg ggcctcgagg
      241 cgttgaagaa tgtcgacctc gagatccgcc gcggcgagat attcgcgctg ctcggcccca
      301 acggcgccgg caagacgacc ttgatcagca tcgtctgcgg catcgtccgg gcgacttcgg
      361 gcgaggtcaa ggcggacggc catgacatcg tccgcgacta tcgcgcggcg cgcagcatga
      421 tcgggctggt gccgcaggag ctcaacaccg acgctttcga gagcgtctgg gcgacggtca
      481 ccttcagccg cggcctgttc gggcgcgccc ccgatcccgc ctatatcgag aagctgctgc
      541 gcgacctgtc gctttgggac aagcgtaaga cggtgatcaa ggagctttcg ggcggcatga
      601 agcgccgcgt gatgatcgcc aaggcgctga gccacgagcc cgacatcctg ttcctcgacg
      661 agccgactgc cggcgtcgac gtcgagcttc ggcgcgacat gtggcagctg gtgcgcgacc
      721 ttcgcaccaa gggcgtcacc atcatcctca ccacccatta tatcgaggag gccgaggaaa
      781 tggccgaccg cgtcggcgtg atcagcaagg gcgagctgat cctggtcgag gagaagacgg
      841 cgctgatgca gaagctcggc aagaagcagc tgacgctcaa cctcagcgag ccgatggcga
      901 cgatcccggc cgagctcgcc gagtggaagc tggcgctcaa ggcggacggc aacgaactcg
      961 aatatgtgtt cgatgccaat gaggagcgga ccggcatacc ctcgctgctg cggcggatga
     1021 gcgacctcgg catcggcttc aaggacctgc acacgcggca aagctcgctc gaggacatct
     1081 tcgtcagcct cgtcaccgac cgggccgcgg catgagcgcc gcgcccgcct tcaacgcgcg
     1141 cggtgtctgg gccatctacc ggttcgagat gcaccgcttc cgccggacgc tctggaccgg
     1201 gctcgcggtg ccggtgatca ccacatcgct ttacttcatc gtgttcggct cggcgatcgg
     1261 atcgcgcatg accgagatcg gcggcgtgcc ctacggcgcc ttcatcgtgc cggggctaat
     1321 gatgctgtcg ctgttcaccg agagcatctt caacgcctcg ttcgggatcc acatgccgcg
     1381 cttcaccggc accatctacg aattgctctc ggcgccgctg tcggcgttcg agacggtgct
     1441 cggctatgtc ggcgcggccg ccaccaaatc gaccatcgtc gctttggtga tcctcgccac
     1501 cgcgcatctg ttcgtcgacc tcacgatcgc gcaccccgtg ctgatgctcg cctatttgct
     1561 gctggtggcg gcgaccttct gcctgttcgg cttcatcgtc ggcatctggg ccaaggggtt
     1621 cgagcagctc caggtcatcc ctttgctggt cgtcaccccc ctcaccttcc tcggcggctc
     1681 cttttattcg atcgacatgc tgcccgagcc gtggcggacg atcaccttgt tcaacccgat
     1741 cgtctatctg atcaacggct tccgctggac cttcttcggc gcggccgacg tgccggtggc
     1801 gtggagcctg ctggcgaccc tcggcttctt cttcgcctgc ctcgcggtag tcgcctggat
     1861 tttcaagaca ggataccggc tgaagacgtg attgtccccc ggccaggagc agatatcggc
     1921 ttagttgcac cccggcgaaa gccggggtcc agcggcgcag aaggctggac cccggccttc
     1981 gccaggcaac agctttaggg cggcgggatc actggacccc ggctttcgcc ggggaacggg
     2041 tttagcgcgc ggcggcttcg tagcgttcgg agaccttgtc ccagttcacc acgtcccacc
     2101 aggcggcgag ataatcggcg cggcggttat tgtacttgag ataataagca tgctcccaga
     2161 cgtcgttgcc caggatcggc gtgccctgca cctgcacgat gtccatcagc ggattgtcct
     2221 gattgggagt ggcggtcact tcgaggctgc cggagccgtc gaccagcagc cacacccagc
     2281 ccgagccgaa ctggccgacg ccggcgtcgc cgaacttctt cttgaacgcg tcgaacgaac
     2341 cgaattcggc gtcgaccgcc tcggccaggc gcccgctcgg ccggccggta ccgccggggc
     2401 ccatcgtctc ccagaagaag ctgtggttcc aatggccgcc gccattgttg cggaccttgc
     2461 ccaactgcga ggagatcgag gcgaggatat cctcgaggct cctgccctcc agcgccggat
     2521 cctcggcaat gccctcgttg agcttgtcga cataggcctg gtggtgcttg tcgtggtgaa
     2581 gctgcatggt ggcggcgtcg atggtcggct cgagcgcgtc gaagccgtag ggcagagggg
     2641 ggagcgtgaa gggcatgcat ttcctcctat tgggtcgggc ccccgctagc cctcccgaca
     2701 gcaagttgca gccgcttttt ttcgatcagc gccggcgccg ccttcgatca gcgccggcgc
     2761 gcgcgtcgag actagttctg caccacctgg aagcggccga gctcggcggc cggggccttc
     2821 cacttgggag cgagcaaatt ggcctgctta tagtcggcat aggcggcgtt gacgttgccc
     2881 gacagctcat aggccatgcc gcgcccgaga taagcgagcg cgggtttctt ggtccgcatc
     2941 tcgatcgcgg tggtgaacag cggcaccgcc tcgccgaccc cgccaggctt cctcagcatg
     3001 acgacgccct tgttcagata ggcctcggcc tggttgccgt cgcgggcgat ggcgacgtcg
     3061 aagtcgcgga tggcggcgtc atactggccc ttgtggaagc gaacgatgcc gcgattgaca
     3121 tgagtcgcga cgatgtcgcg gggcgatagc acgtcctcgg tcagcgcctt gtcgcagaag
     3181 accgccgcct ggttggtgac cacgcccgac tcggccgctt cgtaacagat ccgcgcggag
     3241 ctgcttccga cgacgaagac ggcggccagc gccggccccg cgatcgcggc tgcagcaaca
     3301 gaaaaggcca atgcagagac gaacttgttc atcgcgacct cctgagctca gtgcgtcggc
     3361 atcttatcat ttttccggcc cgcggcctag ctcggcccgg acccgcatgc cccgctagca
     3421 tgtcgttgat cggtcagggg cggacgttgg tcgaaaagtc acgcttcgac cggctccgac
     3481 cggcgatctt cctcctggcc cggtccgccg ggggcggtgc tcatctggtg ccgcttcatg
     3541 gcatggcgga gctggtcgta gctcaattgg agcgccgccg ccgcggcccg ctggttccag
     3601 cggcatttct cgagcgcgtt cgccaggatg gccttttcat attccgccac cgccagccgg
     3661 aaatcggcgc agcctgcggg atcgtagggc gaaacggcgc cggtcgacgc catttcgagg
     3721 cggggcggca ggatttcgag agtcgcatgc tgcctctccg gcagcgggcg ccagggcgat
     3781 tcgaacggat cgaactgaat cgcgtcgatc gggttctccg ggtccgccca gcgatagacc
     3841 gcgcgttcga cgacgttgcg gagctcgcgg acgttgcccg gccagtcgta attgagcagc
     3901 cgggcggcgg cggcggtgct gaagcccggg aagcgcggcc agtcgagctc gagcgccatg
     3961 cggcgggcga aatgatcggc gagcaccgga atgtcgctgg accggtggcg gagcggcggc
     4021 aaagtcacca cctcgaacga cagccggtcg agcaggtcgg cgcggaagcg gccctcgtcc
     4081 accagccgcg gcagatgctc gttggtggcg gcgacgatcc gcacgtcgac gcgcatcggc
     4141 ttggaggcgc cgatgcgggt gacttcgcca tattcgaccg cgcggagcag ccggtcctgg
     4201 gccgggctcg acagggtcgc cagctcgtcg aggaacaagg tgccgccgtc ggcctcctcg
     4261 aatcggccct gccgcgccct ggtggcgccg gtgaaggcgc ccgattcgtg gccgaacagc
     4321 tccgcctcga tgagggtctc gggcaaggcc gcgcagttga gcgtcaccaa cggtccgtcc
     4381 caacgcgacg acaggtgatg gagccgctcg gcgatcagct ccttgccggt gccgcgctcg
     4441 ccgatgacga gcacaggccg gtcgagcggc gcggcccggc tcgcgcgctc gaccgcgtcg
     4501 aggaacgagg aggattggcc taccatctgg gttgtgcggt ccatggccaa aggttggcgc
     4561 aattcaccac ttcttggcaa gccctaaaag gcgccggagc cgtcattctg agccattttc
     4621 atcggtttcg cgccatttcc gcgatctggc acggctcctg cttaagagat ggcataccgc
     4681 ttcgaaaagg cggacgggac caactcagga cttcagggag actatcatgt tcaattcgac
     4741 caagaccgaa ctgcagcgga tcgccgcggc cgccctcggc gcgctgatcc tcaccaccgc
     4801 ctgcgtcggc gccgccgtcg gcccggcccg cgcggtcgaa accgccccca ctcaggtgtc
     4861 ggcgatcgcc tgatccagtg ctaagatcgg ccgaacacac tgatcaccac caggagcttt
     4921 aaatgggtat cttctcgcgc acccgcgaca ttgtcgccgc caacttcacc gaccttctcg
     4981 acaaggcgga ggacccggcg aagatgatcc ggatgatcat catggagatg gaggagacgc
     5041 tcgtcgaggt gcgcgcctcg gcggcgcgga cgatcgccga ccagaaggag atgcgccgtc
     5101 agatcgccaa gctcgaccgg ctccaggaga gctggaccga gaaggccgag ctcgcgctgt
     5161 ccaaggatcg cgaggatctc gccaaggcgg ccctggtcga gaagcagaag gccggcgaca
     5221 tggccgacag cctgcgcgcc gagatcgccg tcctcgacga cggcctgcgc gcctccgagg
     5281 ccgacatcgc caagctccag agcaagctgc gcgaggcgag aacgcggcag aatgcgatcg
     5341 tcagccgcct cgaaagcgcc cagaatcgca gccggcttcg cgaaatgtat gccggaccca
     5401 aggtcgagga tgccttcagc cgtttcgaag tgctggagcg ccgggccgat ttcgccgagg
     5461 gccgcgccga cgcgctggcg cttggagcgc cgccgaagac gcttgaagag gaaattgccg
     5521 aactccgctc gtcggagaag gtcgaagccg agctcgccgc cctgaaggcg aagcgggccg
     5581 gcgcaacggg cggggaggcc tgatcagatg gaagaagcaa tcgtaggtat catcgccggc
     5641 tgcatcgcga tcgtcacctt gttcgtcggc ctgccctggc tgatcttcca ctatgtcacc
     5701 aagtggaaga gccaggcgac gctcaccgcc gaggacgaga atctgctcga cgatctgcac
     5761 gatctcgcgc gccggctcga cgatcggttg gcgacggtgg agcggatcat gaccgccgag
     5821 aacccggcct ggcggcagct ggccgcggat ccggccggca tcggcatcga cgacctcagc
     5881 tcggtccgga ggctcagcaa atgaacgccc cccgccgcac taaattctac ctcgaccgcc
     5941 gcaaccgcaa gtggaagggc gtctgcgcgg gcatcgccga ttataccggc gtcgacgtgg
     6001 tggtcattcg catcggcctc gtcatcctga ccttcgcggg cggcttcccc tggacgctga
     6061 tcgcttactg gacgaccgcc tggatggcgc cgcacaagcc gcgcgagctc tccgatgaga
     6121 gcccggagga ggcgaaattc tggcagggcg tgcgcgcttc gcccgcccgc accgtgcgcg
     6181 acgtgcgctc gcgcttccgc gacatcgacc gccggctggc cgacgtggag acctatgtca
     6241 cgagctcgaa cagccggctc gcgaacgaga tcgaaagcct gcgctgaagg cgcctgatca
     6301 gggggagaca acaaagtgac accggaaaag atcgaaaagc tcgccttcat cgcccagctt
     6361 ttccctttcc tggcggtgat cgtcagcgtc gccatcggcg gctgggtgct gacgacctgg
     6421 ctcaggatca agaacggcta cccgctcgac ggcatgtggg gccaggccgt ctatccgaag
     6481 aagaatgagg aagcgatgga gcggatcaag ctgctgagct ccgaaaacgc ccagctccgc
     6541 gccgagctcg gttcgatcaa ggaccggctc gccaatgtcg agcgcatcgt caccgacgac
     6601 agccaccggc tgacgcagga gatcgagtcg cttcgcgtcc cgcgcaactg aggaaccacc
     6661 ggtaaactga aggcgccggt caactgaagg cgccggtcaa ccgaaggcgg cagccctggg
     6721 ggagagagat tttgctgagt tcgcacatca tggtcgtgct gatcgtcacc atcgcggtgg
     6781 tcggcagcat cttgcgcgcc cgctacaagg ccggcgccca ccgcagcatc gccgacgatc
     6841 ccgaggcgca gcgcctccgc gacgaggtca agcagctcaa ggatcggctc gcggtgctcg
     6901 agcagatcac catcgagaag gaaaacagcc tggagcgcca gatcgaggcg ctgcgcgacc
     6961 gctgaggcgg cggcggggag gctttgcgga cggcgcggct tggaatgcgg cggcgacgcc
     7021 attaggtggc ggacatgacc gcgctcggcg acatttacga ggattatgag ctgctcgaag
     7081 cggacgaccg ctaccggctg ctgatcgacc tcggccgggc gctggagccg atgccggacg
     7141 cgctcaagac cgatgcgacc ctggtgcggg gctgctcggc ctcggtctgg gtctacccga
     7201 cccagcttgc cgatggccgg ctgcatttcc tcgccgacag caacgccgcc attaccaagg
     7261 gcatcatcgc tttggtgctg gccgcggtgc aggaccggag cgcggcggag atactcgggg
     7321 acgacatcgg agcggcgctg gcgccgttcg acctcggcaa tcagttgagc tcgaaccgca
     7381 cccaaggtat ccccaacatg atcgcgctga tccgcgcgac ggcggagcgg ctcgacgggt
     7441 gagcgcgccc gacctctggc gggacgggtt cgacgtcaag gcgttcatgg ccttcgtcga
     7501 aaagctcggc catggcggcg cgctcggcat ccgctatcac ggccatgccg ccggatgggt
     7561 cgagctcacg ctcgattatg acgagaaact ggtcgggatg gtggagagcg ggatcatcgc
     7621 gtcgggcccg atcatcagcc tcatggacat ggcgaccagc ctcgccgtct gggcgaggct
     7681 gggccggttc cgcaaccagg ccacgctcga catgcggatc gactatatgc ggccggccgt
     7741 gccgggacgg gcgatcgtcg gccgcggcga atgttatgcg gccacccgca gcgtcgcctt
     7801 cgtccgcggc ctcgcccatg atggctacgc cgccgatccg gtcgcccacg tcaccgccac
     7861 cttcatgttc accgaccagc cggctcccga agcgggcgga gcttagctcg aaagcgagcc
     7921 gcgccgctct tcgctcttgc gcaacacctc ataggccgcc tggatcgcct ggaaccgcga
     7981 ggcggcctcc gcgcttggcg cgacatcggg gtgattggcc ttggcgagcc ggcgataggc
     8041 ggccttgatc gcgtcgaaat cggcgtccgc ttccagttcg agcacgtcga gcgctcgcat
     8101 ctcgtcgcgg ctgcggctgc cgtcgccggg gccgccccag ccataatggc cggtctcctt
     8161 atagccgcca tggtcgcgcc gctcgtcggc ttcgcgcgcg gccgcctcct cggcgctgag
     8221 cccttggaaa taattccagt tcttattgta ttcggccgcg tgccgctcgc agaaatacca
     8281 gcggtccggc ttgttgggag ccttgggcgc gggccggtcg ccgggctcgg tgcagccatg
     8341 gcggtcgcat agccgcacct gctgcgccgc gcggtcgctg ccatagccgc gccagcgcgg
     8401 aaatccccaa tcgttcgaac gcgtctgcct ggtcattagc gggcagatag agactgtcgg
     8461 cggcgagggc cagcggcctc gcgccttccc ggacggaaaa gcgtcgaaag gcggctggcc
     8521 ggcccgaccc cggcccgaca ggacccgcga tagggcggat cagtagcgat aatgctcagg
     8581 cttgaacggg ccttcgaccg gcacgccgat ataggaggcc tgcttgtcgg tgagcttggt
     8641 gagcttgacg ccgagcttgt cgaggtggag cgcggcgacc ttctcgtcga gatgcttggg
     8701 cagcacgtag acgtcgttct ggtatttgtc gccgccggtc caaagctcga tctgcgccag
     8761 cacctggttg gtgaagctcg acgacatcac gaagctcgga tggccggtgg cgttgccgag
     8821 attgaccagg cggcccttgg acaggacgat gatcttcttg ccgtccggga actcgacctc
     8881 gtcgacctgc ggcttgatct cggtccactt catgttctgg agcgcggtga tctggatctc
     8941 gctgtcgaaa tggccgatgt tgcacacgat cgccatgttc ttcatcgccc gcatatggtc
     9001 gacggtgatg acgtcggcat tgccggtcgc ggtgacgaag atgtcggcgc gggtgaccgc
     9061 ttcttccatg gtgatgactt cgaagccctc catcgcggcc tgcagcgcgc agatcggatc
     9121 gacttcggtg acgaggacgc gggcgccgcc gttgcggagc gactgggccg agcccttgcc
     9181 gacgtcgccg aagccggcga cggtggcgac cttgccggcg agcatgacgt cggtggcgcg
     9241 gcggatcgcg tcgaccagcg actccttgca gccgtagagg ttgtcgaact tcgacttggt
     9301 gacgctgtcg ttgacgttga tcgccgggaa cggaagcttg ccctgcttgg acagctcgta
     9361 aagccggtgg acgccggtgg tggtctcttc cgacacgccc ttgatcgctt cgacggtgcg
     9421 ggtgaggaag ccgggcgact gctcgatgcg gcggcggacg acctgctgga agacttcctc
     9481 ctcctcattc tgcggcgccg gaaattcctc gccggcctcg atgcgcgcgc cccacaaggc
     9541 gaacatggtg gcatcgccgc cgtcgtcgag gatcatgttg gtggtctcgg tccaggtgcc
     9601 gccggggccc caggcgaaga tgcggtcgat atagtcccaa tattcctcga gcgtctcgcc
     9661 cttgatggcg aacaccggca cgcccgacgc ggcgattgcg gcggcggcat ggtcctgggt
     9721 cgagaagatg ttgcacgaag cccagcgaat gtcggcgccg agcgcgatca aggtctcgat
     9781 cagcacggcg gtctggatgg tcatgtgcag cgagccggtg acgcgcgcgc ccttcagcgg
     9841 ctgcgaggcg ccatattcgg agcgcagcgc catcaggccc ggcatttcgg tctcggcgat
     9901 ctcgatctcc ttgcggccga aagcggcgag atcgaggtcg cgaacgacat agtcgttgaa
     9961 cggggtctgg ggctgagtcg ccacggatga gctcctgaat cgaggatgga agaggctgga
    10021 aaaccgcggc ccctttagcc ccggaacgcc acagatgcaa atataaagat atctttatat
    10081 gcggccccca cgcgccgaag atcacactaa aatcacacca actccgcctc aagacactgc
    10141 tctgaccgcc catttcgcct tcaaattggc ttgatagaac aatagcagaa cgcagacttg
    10201 taggacagcc ttcggctcca gcaagcgccc tacgccgtgc cgaagtcggc ggtcagcagg
    10261 cgggtgtcga tgccggcgct ttggaaggcg gtggtccagc gggaattgac gtcgcaatcg
    10321 tagagcagtt cgtcgctgcc gggcgtggtg aaccagccgt tgccgcacat ttcctgctcg
    10381 agctgcccgg cgccccagcc ggcatagccc agagccgaca cccaacgcgt cgggccgcgg
    10441 ccctcggcga tggcgcggag gatgtcgagg gtggcggtga gggtccagcg gttcgagacc
    10501 tggacgctgc cgtcgccgcc ccaatcgagc gaatggagga tgaagccacg ctgcggctcg
    10561 accgggccgc cgaggtggat ggcggaatcg ggagcggtgc cgaccgggat gtcgagctgc
    10621 ttcaagagat cgtggaagcc caggcgcggg atgatccggc cgagcccgat tccgagcgct
    10681 ccgccttcgt catggacgca catggcgatc accgcctttt cgaagcgcgg gtcgccgatg
    10741 ccgggcatgg ccagcaggaa ctggccggtg aggaaacgag gctcttcttg catcgtcccg
    10801 gctataggct cggctcgccg cccctgctag ggcgcgggcg acttccatgc ctccagacga
    10861 aaggaacagg cgatgagcat cagcgtcggc gaccgcattc ccccgatccc cttcatcaag
    10921 gcgacgccgg aggggcccgc gccggtcgag tcggagaatt atttccgcgg ccgcaaggtg
    10981 gctttgttct cggtgccggg cccgttcacc ccgacctgct cggcgcggca cctgccgggc
    11041 ttcatcgaca aggccgatgc gctgaaggcc gccggcatcg atgagatcgc ctgcaccgcc
    11101 gtcagcgacg ccttcgtgat gcaggcctgg gccgagcagt ccggcgcggc cggcaaggtc
    11161 accatgctgg ccgacggcaa cggcgcgttc gtgaaggcgg tcgggctgga aatggacgcg
    11221 tcgaaattcg gcatggggca gcgcggccag cgtttctcga tgctgatcga cgacggggtg
    11281 gtgagccagc tcaacgtcga ggagccgggc gcgttcaacg tcagcagcgc ggactatctg
    11341 ctcgaacggc tgtgaggcgg cggggccgag gccgatccgt cccttccctg cctctcctga
    11401 acggcgtaag ggcaactaac tcgactccgc ggcattgatg cggattgtac agcgagttca
    11461 ggagattggg atggctgagg gccgcacccc gcatgaggcc ggcgatagcg ggcgatccga
    11521 ttcttcggac ggcagcgatg ccgagttcgt ggccggcggc gcgcaatggc atccgatgaa
    11581 ggcggcggct ttggtcggcg gcgcgatcgc cgccggcgcc ggcgcctatc tcggcacgcg
    11641 cgcgctcgcc cgccgcaacg cgagctcgga cggccgcaag ctcaattcgg tgatggcgac
    11701 cgcgatcacc gcctgcaacc tggcgcagaa gcagccggcg acggaggccg agccggcggt
    11761 ggcgccggaa ccgacggtgc cgcgcgaacc gcgcgtctga ctatctgcgc gcctcgagcg
    11821 ggcgcagcac cttgaccgcg aagctttccg cctgctcggc gaagaccagc aggttgaccg
    11881 ccaaattgcc gcggacgaag ccgtaatcgg cggtcagata gcgctggagc catggcttgc
    11941 cgcccggcgc cacgaaggcc ttggcaaagg cgaagctgcg gttgaagccg tcgatcgccc
    12001 tttccgtccg ctccgggggc gagttggcga tccgggtcca ttcggaatga atgctcagcg
    12061 acttgcaggc ggtgccgcca ggattgcaca tgtcgaagct gaggatcgcc gtgcggctgt
    12121 tgggcagcac gacgtggagg accggctttt ccggcgaggc ccccgcgcgc cggtagcggg
    12181 cgccgatctg cgccagcacc ggctcgacgg tggcgtagct gaaggccggc aggagctgcc
    12241 ggttctcggg cgccgcggga ttgaagcgcg cggtcggcag cgcggcccct gcgggggccg
    12301 cggcggcggc gagcagggcg gcggtagcgg ccgctttcat gcacggtcct tcatcgtctt
    12361 cggagtgagc ggggcgcccc ccactcgatc gtggcttagg acgcggtggc cgccgtgccg
    12421 gccgcccagc cgcagcgccg gccgcgattc tgctgttcga gccaggccga caaaggccgg
    12481 aaatattcga gcagcggctc gcccgacatt tcgcggctgc cggtaaaggc ctggagcgcg
    12541 tcgggccagg gcttggaagc gcccatctcc agcatcgcgt tcagccgccg gccgacctcc
    12601 ttgttgccgt agaaggagca gcggtgaagc ggccccttcc agccggcctg gtcgcaagcc
    12661 gccttgtaga attggaactg gaggatgcgg gcgaggaagt agcgcgcata aggcgtgttg
    12721 cccggaatat ggtatttggc gcccggatcg aactgcgcct cggtacgcgc caccggcggc
    12781 acgatcccct gatattcgag ccggaggtcg ttccaggcct tgttgtagcc gctcgccggg
    12841 atcgagccgc cgaacacgcc ccagcgccat ttgtcgacca tcaggccgaa cgggaggaag
    12901 gcgaccttgt ccatcgcctg gcggaggagc aggccgatat ccttgtcggc ggacggcacc
    12961 ttgctcttgt cgagcagccc gacctggacc agatattcgg gcgtgatcga gagcgcgacg
    13021 gtgtcgccga tcgcctcgtg gaagccgtca ttggcgccgt tcagatagag gaacggctgc
    13081 ttgttatagg cgcgctgata ataattgtgg ccaagctcgt gatggacggt gacgaagtcg
    13141 tccgaattga ccttggtgca catcttgatg cgcaaatcct cgacattgtc gacgttccag
    13201 gccgaggcgt ggcagaccac ttcgcggtcg gccggcttgg tgatcatcga gcgttcccag
    13261 aaggtcgccg gcagcggcgc gaagcccagc gagctgaaga agccctcgcc ggtcttcacg
    13321 atcttcacgg ggtcgtactt cctggcgacg agcaattcgg tcacgtcata gccgatgtcg
    13381 ccggcgccct tcggagcgac gacgtcgtag atattgcccc attcctgggc ccacatgttg
    13441 ccgagcaggt cggcgcggat cgggccggcc gccggctgca cctcattgcc atatttctca
    13501 ttgagcttgc cgcgcgtgta gcaatgaagc tggtcgtaga gcggcttgac ctggccccac
    13561 agcttgtcgg tgagcgcggc gaactcgtcg gcgggcatgt cgtagcccga gcgccacatc
    13621 gcgccgacat ccttgtagcc gagctccttg gcgccctcgt tcgagatctg gaccagccgc
    13681 tgataatcct tgcgcatcgg cgtaccgaca ttgctgtgcc agctggtcca catctcctgg
    13741 agctcggccg ggttgcggtt ggtgcccatc gcctcttcga tgtcgctgcc gctcatcgcc
    13801 ttgccgttca gcgtgccctt gcccttgccg taggtcgaat tgaggcgggt ggcgatctcg
    13861 ttcagctcgg cggcggcgcc gggggtggtc ggggcgggca ggacgatgcc gccgcgcagg
    13921 atgtcgagct tgcgcttggt gtcgtagctg aggccgcgga cattggcgta gcgcgccgcc
    13981 tcgttggcca ggcgcacaga catctcggtg ccgatggtgc cgaaatgcgc ggccaccgcg
    14041 tccgagtcgt cgttgagata ggtgttgttc acccattggg cgcgcgagct gatgttcgag
    14101 aattcggcga gctccttctc cgcccgcgcg acgaaggcgt cggcctcggc ggcggtggcc
    14161 ggcccggcct tggcggcggc gggggcgcgg cgctgctgcg ctccctggtt cgagctttgg
    14221 gcgaaggaag cgccgcccac ggcgagcagg gtcgcgacgg cgagcgcgga tacggtggct
    14281 ttcatggcat cctcggggct gatggtttgg ttcgtcttat aagctgctgg cctgaacgca
    14341 agccggtcaa cgagcaagga aatccgcgat ggcgccgccg agctcgggct tggtgacggc
    14401 gctcatatgg ttgccgggga tttcgacgta gcggccgtcg gcgagcgacg ccgccagctc
    14461 ctcggaggag ccgttgtcgc gatcctcggc gccggcgacg accagggtcg gcatggcgag
    14521 gcgggcgagc tcatcggcgc ccgtgtcgac gaagctgccg agcaggggca gcaaagcctg
    14581 cggatcgccg cgggtggtct tcaaaaaggc ttcggccatc cattccggcg agccgcgctc
    14641 gaagctgccg aggccggtga ggatgcgccg gaaatgatcg gcccggccgg cggtgccgag
    14701 caggccctgc agccccatgc cgctgacgac cagccggtct ggggcgccgc catggaccac
    14761 catccgaacc gccgtcctgg cgccgagcga atagccgccg agatcgtagc cggtaaggcc
    14821 gagatgggcg atgagcgcga gattgtcgcg gacgagcacg tccggcggat aggcggtctc
    14881 gtcatgcggc gtggcgctgc ggccatgcgc gcgcaggtcc ggcatgatga cgcggaagtc
    14941 gcgggcggca atcgcggcgg cgtggccgta gcggatccag ttggtgtcgg cgttggagaa
    15001 caggccgtgg atgagaatca ccggtcggcc ctcgccaagc tcggcccagg cgatctcgac
    15061 cccgtcgaac gagccgaagc tgtggcggcg cggatcagcc ggggaggttg ccaactttgt
    15121 ccatccaacg gttgaccagc ggctggtaat cctcgctgcg catttccggc agcccgcttg
    15181 tgttgagcca ggcgcggtgc atgctctcgg cgccgaaatg gtgtttgggc acgaacggcg
    15241 agggatcgtc gaacgtgccg acgatcaggt ccagcttgtc ggtcccctcc acatattgga
    15301 aggtcagcga cgtgccgcag gcggagcaga agccgcgccg cgcgatggct gaggattgat
    15361 aatattcggg ctcccgctcc caggtcacat ccgactgcgg cagcgtcttc atcgccaggc
    15421 tgacattgcc gctcgcccgc tggcacatcc ggcagtggca gagataggcg tcgtgatttt
    15481 cgatccgggc cgagtagcgc acgcgcccac aggcgcagcc tccgctaact tccttcattc
    15541 aaaatgcctc cactttctcg gagagaccgg cgcgagccaa agcggcggcg aatttctcgt
    15601 ccgccgtcac cagcttacag ccttcccgct cggccaaagc cagatagatg caatcgtaga
    15661 cggggtgacg taaaccgatc gaccagcgga acgcatcctc gatgagatcg ctcgcgggat
    15721 aaagcagctc gaaaaactcc ggcagatcct gaagatgctg cacggcgtgg tcggccagca
    15781 actccgacct cttcaccttt ttccagaatg tgttcgcagc ttccacaagc agccaatcgg
    15841 gcgcgatcag cggatcgggg atcgacaata accgacgggc ctgatcattg ccgggctccc
    15901 ggaccaggaa tttgacagcg acgctggcat caacgaccag cggcatcgtc gtcccagccg
    15961 tcgtcgatcc agcgtccgcc gccggtgtcg cgatcccagc gaattagcaa ggtgctgtca
    16021 ctggcctcgc cgagtccatc cggcggcgtc agtgccaatg catcgtcgga aatccgaagc
    16081 agatcggcag aatttttggt tcgcttaggg caatgagtct cgatcacctc ccgcagctcc
    16141 gcctcgaggg accgcccctt ggctttggca gccttgcggt acggggccag agtgccgggc
    16201 ttcaatcgtc tgatgaccac ctgctccatt gctcgatgat atcaaagtgc catcaatcgc
    16261 tcaagccgcc ttgcggagat gcttccttcc gagcagttcg gcgatctgga cggcgttgag
    16321 ggcggcgccc ttgcgcaggt tgtcggacac gcaccagagc gaaaggccgt tgtcgaccgt
    16381 ggaatcctcg cggacgcggc tcacataggt ggcatattcg ccgacggcct cgaccggcgt
    16441 gacgtagccg ccgtcctcgc gcttgtcgac gagcatgatg cccggcgact cgcgcaggat
    16501 gcgctgcgcg tccttggccg aaatctcgtt ctcgaactcg atgttgatcg cctcggaatg
    16561 gccgacgaag ccgggaacgc ggacgcaggt ggcggtcacc ttgatcttcg ggtcgaggat
    16621 cttcttggtc tcgaccacca tcttccattc ctccttggtc gagccgtcgt cgaggaagtc
    16681 gtcgatatgg gggatgacgt tgaaggcgat ctgcttggtg aatttatgcg gctcgttcga
    16741 gtcgccgacg aagatgttgc ggctctgctc gaacaattcg tccatgcccg cctttccggc
    16801 gccggagacg gactgatagg tcgccaccac cacccgcttg atcttcgcga aatcgtgaag
    16861 cggcttcagc gccaccacca tctgcgcggt cgagcaattg ggattggcga tgatgttgcg
    16921 cttcgtatag cccgcgatgg cgtgggcgtt gacctcgggc acgatcagcg ggacgtcggg
    16981 gtccatccgg tagagcgagg aattgtcgat caccgtgcag cccgccgccg ccgcgcgcgg
    17041 ggcatggagc ctggacactt ccgagccagc cgcgaacagc gccatgtccc agccggcgaa
    17101 atcgaaatgg tcgatgttgc gcaccttgag cagccgcccg ctgtcgccga aatcgatctc
    17161 cgtgccgctg gagcggggcg aagcgacggc ggcgacttcg tcggcgggga attcgcgctc
    17221 ggccaggatg ttgagtatct cgcgcccgac attgccggtc gcgccgacca ccaccacccg
    17281 ataacccatg ctgcttctcc agatatccag ccgccgatct aatgcgggcg cgccgcttat
    17341 ccaaggaccg agcggaatgg gataggttcc gggatagggc gacaggaaga agtgagaccc
    17401 cggatcaagt ccggggtgac gatcaccccc gatcaagtcc ggggtgccag tctattccga
    17461 tcaacaggct ctagcgcaga accgagcggg cgagcctttc gaggcccgac gcgcgggcgg
    17521 cgcgcttggc gagggcgacc gcggcgtcga agcccatcag cgtgtggccg acgatcaaat
    17581 tggcgggggt ccggcgcacc agcagcaggt cgacgcaatc aagcgcgccg gtcgcgaact
    17641 ggcgcttatg ctggccgtcg ccttcggtga agtcgaacag ccggaagccg ccctcctcca
    17701 tcaagccgcg catggcctcg agctgcagca ttgtgccggg cgacaacgcg gcgtgagccg
    17761 ggtcgtagcc gagataggcg tagatcaggg tcgctccctc ggccggcgtg tagaggtagc
    17821 tgaccggctc gccgccgagg aacaggaccc agccgcgcat cgcgccgcgg cgggcgcgct
    17881 cgcgcatgtc ggccagcgcc tgctccccgg agggcaggcc ggcgtcgagc agcttctcct
    17941 gataggtctt ggcggagacg gcacgagcgt gacggtggaa cgcctcgacc tcgtccggag
    18001 cgctgtagcg gcgcagatcg agccgccccc ccgacgcgtc ggcgaacttg cgcagcttgc
    18061 gcttgagggt ggagcgggtc ttcgacgaga aaccggcgag ataatcgtcg aaggcgatgt
    18121 cgagcgccgc atagctgcgc tggtagcgtt gccgcacgaa gcatttgagc tcgggatgcg
    18181 agacctgcac ccgatcctgg agcgcggccg gaagcgcgta gatcatatag ccatcggcac
    18241 cgcggccaag cggaggcaag gccggcgccg tgccgctcat cccctgttcc agcggcagcc
    18301 ccacctggat cagccggcgc ttcacccgcc agatggtgcg agcgccgatc cgcagcggca
    18361 gcgcgacgat tgcggtcaca gcgcccgctc gtagacggcg ttcgcccaga gctgctcggc
    18421 catgcggcgc gcgcggcgca ccggctggct cggcagcggg gcggcggctc ggccgagcgg
    18481 caggccgccc agatccgtga aatgagcggt cggcagctgc gcccgctcgt cggcgaggaa
    18541 ggcgcaaagc cgctcgaagc ggagcttcag caccggattg ggccgcagcc cgtcgcgggt
    18601 ggcgagctcg aagctgtggc tgacgatggt ggtgagcgga tggccatggt cggccgcgtg
    18661 gagcaaagcg gccctgaatt ccgagaagga caaggcggtg acctggaggt ggcgcagctt
    18721 gccgtcgcca tcctcgatct gcccgaccgg gacttcgacg acgccgcgat gctcgaccgg
    18781 ggcgatcagc gccggatcga gcggcagcgc gctcggccag ggatgatggc tgccattatg
    18841 gctgctgtcg tagcggatgc cgaggctggc gagcgcatca agcgtgatct cgtcggcggc
    18901 atagctgccc gagcggaagg cgaccggcgg cggcgcgccc gcctcgacca ggagatcgcg
    18961 ggcggtggcg atgaggtcgc gctggtcaag cgggttgaag ccggtcagct cgaacttgcc
    19021 ctgctcggcg ccgccattgg cgaggcaatg ccagaagctg tggagatgga gctgcacctc
    19081 ctgccccgcc tccaggatcg gcgccaccat gcgccgcacc ggctccagcc cgtagacgag
    19141 cgccggcatc ggatcgacga agaagcaggc cttgagcccg tgcgcgcgaa gcagatcgag
    19201 ctggtagggc acgccggccc cggccgcttc ataagcgcag gcgaaattct cctgccacga
    19261 agcgccgcgg gcatagtgcc gccacgtcag ctcggtgtcg acggtgagga ggacaggggc
    19321 aaccatgatg ccccccttag ccgcagcggg tgaaagaaag gtgagcgacg gcgcgggaac
    19381 gggcgtcggg ccttgggatg gcctggggtg ccggtatggc ctagcatcgg cggaggccgg
    19441 atccctccgg tcggccaccg gaggaatgaa tgaagatctt gctcgccgcc gccgcgacca
    19501 ttgtgttgct cgcctgctcc gaccagcaaa gccccgccgc caaggcggcc gagggagcgg
    19561 cgccgaaagt ggaggcgaag gtggcgaccg ctgccgccgc ttacgagcct tatctggaga
    19621 aaagatatcc cgcttccgga aaggccgtcg ccgccgccga ggtcgccggg atgatcgagc
    19681 gcgatgggcc gcaaaaggcg gtcgcggcct tgtggggcga aggtgccgac aatggctggg
    19741 aagcggtgat gcgcggcatc gccaagggcg accccgcctg gctggcgctg gctccggcca
    19801 tatccaaggg agccgatgcc ggcgcctcca ccgggctcgc catggcgtcg caggacgcgc
    19861 tcaccaccaa cgccgcgggc gcgcttcggc tgatgtcgca gctcggcgac ggcgccgccg
    19921 gctgcaccta tgaaagctac gaagacccgc ccgagcagag ccgcgccttc tacgccgcgg
    19981 ccacggccgg ggtggaggcc gtgacggatc cggcgctgca gacgacgagg gacgcctgcc
    20041 tcgcccaact tcggagcggc gcggcgcaat cctgatcttc gtgagaaatc gaggcgccgg
    20101 actgacgccg gtccgcgtca gctcccttgg gagccggcgc cgtggcgcgg aaagggagcg
    20161 atgagccggc tatattcggc ttccgcatag ccgtaagtct cgccggccaa ccgccgcagg
    20221 ccgtcgcggt tgcggatcgt gactcgcccg cggcgggagc ggatcagccc ctcgccttcc
    20281 aatatgtgga gcgcatcggt gacgctcgcc cgccgcacgc cgagcatcac cgcaatctcg
    20341 tcgtgagtga ccttgatttc gtccccttcg atccggtcgt gcgccatcaa cgtccagcgg
    20401 cacag
//
LOCUS       DASVRK010000268        13068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_244893, whole genome shotgun sequence.
ACCESSION   DASVRK010000268 DASVRK010000000
VERSION     DASVRK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244893"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VF589_10815"
     CDS             <1..1115
                     /locus_tag="VF589_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258114.1"
                     /translation="QGGAGADTVNGGTGDDTLSSGAMSFDGRGDLTLEVDTLIGQNGN
                     DRIEIGMKDIADGGNDFDTLQLDFTASKLAENFVFSADMTFANGGSAKNFEALTFFGG
                     RFADVVTGGANADTLYGGGGNDLLRGALGNDMIVDGQGRDRMFGDAGADIFRTSGLNA
                     DADIYDGGTDKDTLMFWDGEDDEEVESTMSGFLDLENQALNDGLLKGDTFKNIETFIG
                     AGRDDFMFGNRLANTFIGGDGDDVLDGRAGNDLLVGGEGSDTLTGGLGKDIFDLSGQG
                     NHGAWFGDIVTDFTRGEDKFQISLESLGLEGAPAFTVVVGNNPAPTGSGPQLLFDTAT
                     DRLWFDLDGAGTDHEGMLVATLEGVTTLAVSDFQFV"
     gene            1225..2076
                     /locus_tag="VF589_10820"
     CDS             1225..2076
                     /locus_tag="VF589_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX8258115.1"
                     /translation="MLARPAVAGVREQDVNRETRIDAGRPEELDTWTVAGRSFRSRLI
                     VGTGKYKSFEQNAAAVEAAGAEIVTVAVRRVNIADPKQPMLTDYIDPKRITYLPNTAG
                     CFTAEEAIRTLRLAREAGGWTLVKLEVLGEAKTLYPDMIETLRATEILAKEGFEPMVY
                     CADDPIAARKLEDAGAVAIMPLGAPIGSGLGLQNRVMIRIMVEQARVPVLVDAGVGTA
                     SDAAVAMELGCDGVLMNTAIAEAKEPVLMARAMKLAVEAGRLAYRAGRMAKRRYADPS
                     SPLAGLI"
     gene            complement(2295..3995)
                     /locus_tag="VF589_10825"
     CDS             complement(2295..3995)
                     /locus_tag="VF589_10825"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX8258116.1"
                     /translation="MPLLRKLLAAVTGFALAAAPVAAAAADYDTIIRGGMIYDGQGGV
                     PFKGDVAIEGDRVVAMGDLSGATADREVKADGLAVAPGFINMLSWANESLLQDPRSMS
                     DITQGVTLEVMGEGWSMGPITPTMRADMVKGQSDFRFDVPWTTLGQYLEHLEKKGISP
                     NVASFVGATTVRIHEIGSDNKKATPEQLKRMQDLVRQAMREGALGVGSSLIYAPGNFA
                     DTDELVALTAAAHEYGGGYISHLRSESSRYEQAVEELLTIGRRTGAPVQMYHMKPAGR
                     ENWAKSEPMLRRLWQARAEGIDVTANIYTYTAGATGLYATMPLWVQEGGHEAWVKRLK
                     DPAIRARLVREMRAEPEGWENLLHDAGGPQNVMLIAFKNDKLKPLTGKTLAEVAKLRG
                     TSPEDTIIDLIIEDDSRVGAAFFHMDEANIARNIKWPWTMLGSDSGSVAAEGSFLESN
                     PHPRAYGTFARFLGKYVRDEKVIPLPEAIRRLTSLPAAQLKLRGRGRLAPGYFADVVV
                     FDPKTIADKATFKQPHQYSVGVRHVLVNGVPVLKDGKHTGATPGRVVRGPGWSGWGGR
                     "
     gene            4103..5110
                     /locus_tag="VF589_10830"
     CDS             4103..5110
                     /locus_tag="VF589_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX8258117.1"
                     /translation="MTEKPKSHSPHAWQPDHLRLAVEAAGVALWAWNVDNDRFTMDER
                     GFELWGLPWRDTVSFEELSQHIHPADRDRVRAAFAATRAVLGPYETDFRIIVGEEIRW
                     IAARGQGEDVGIVGRTMFGIFLDVTGRKQAEEGHELLAGEMSHRVKNLLAIAAGLTQI
                     TSRTTASTKDMARELIQRLTALGRAHDLVRPLPKSQGSAALLGDLLSVLLAPYDDLGA
                     FSGRIRVAVERMGVGEAAATSLAMVVHELATNSLKYGALSSDVGTLDISTRSNGDNIC
                     LIWMERGGPPVEAPPEAVGFGSKLVERSVSRHLGGTIEHDWLAEGLVATIQLDRAKLS
                     H"
     gene            complement(5150..5419)
                     /locus_tag="VF589_10835"
     CDS             complement(5150..5419)
                     /locus_tag="VF589_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258118.1"
                     /translation="MSMPRFYFHVCNGNGFTEDSGGKVLPDLPAARQEAITSLRSILA
                     ADMNMGHVNMSSFIEIEDDEHRLMMTIQFMDAIEISTERCSRVRA"
     gene            complement(5525..6706)
                     /gene="purT"
                     /locus_tag="VF589_10840"
     CDS             complement(5525..6706)
                     /gene="purT"
                     /locus_tag="VF589_10840"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEX8258119.1"
                     /translation="MTATARILLLGSGELGREFTISAKRLGCHVIACDSYAGAPAMQV
                     ADESEVFSMLDGERLRAAVARHNPDYVVPEIEAIRTEALAEIEAAGTVVVPSARAAQM
                     TMNRDGIRELAAGGLGLRTSRFRFAESLDEVREAAGHTGFPCVIKPVMSSSGKGQSTV
                     ADAAGLEAAWHYAVANMRGDRARVIVEEFVRFDYEITLLTIRTRDGVLFCPPIGHRQE
                     RGDYMESWQPAAMSAAALASAQGMARKVVDDLGGYGLFGVEFFVAGEEVIFSELSPRP
                     HDTGMVTLISQNLSEFDLHARAILGLPIPEVALRHAAGASAVILADREAEHFSIGGLA
                     DALAFGGAGGEVDVRIFGKPVTRPNRRMAVALASGATTDEARRLAAEAAAKVRISYAS
                     V"
     gene            6886..7941
                     /locus_tag="VF589_10845"
     CDS             6886..7941
                     /locus_tag="VF589_10845"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8258120.1"
                     /translation="MPKLKPLAEQVIVITGASSGIGLATARMAAEAGARVVLSARSRE
                     ALAQAVDEIKQAGGQAAFVAADVTRRGELEAVAAEAVDRFGGFDTWVNNAGVGIWGLI
                     DEVSEEDMRQLFETNFWGMVYGSEIAVRHLKAKGGALINVGSLTSDRAFPVQGIYSAS
                     KHAVKGFTDALRMELEAEKAPISVTLIKPASIGTPMPQHVKNYSDQEAKFPPPIYAPE
                     EVAATILRAAERPIRDAFIGSGARTISSLSSKTPGFLDWVSAKFLIPAQYGDQPATPT
                     DNLHHGQAEAKVRGDHQGSLIRPSAYTRAARHPAATLAIAGLAAAGLGALFARRRNRE
                     EEQKLLPAPSGVRAERE"
     gene            complement(7915..8394)
                     /locus_tag="VF589_10850"
     CDS             complement(7915..8394)
                     /locus_tag="VF589_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258121.1"
                     /translation="MRRLYLDCDGVLADFDTGATAILGMKPKAYEKSRGPGRFWSKLA
                     QASDFYFSLPLMPEATRLFEAVRHLDPVILTGLPRGNWAADQKMRWAAQHFPGVRMIT
                     CMARDKRDHAEEGDVLVDDNDKYRHLWEGAGGVFVHHRNVDETLAELALLFPLGADA"
     gene            complement(8396..8815)
                     /locus_tag="VF589_10855"
     CDS             complement(8396..8815)
                     /locus_tag="VF589_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258122.1"
                     /translation="MTLDPQQAHDQAGKGSGVIGWALDEDQRKELLMQFRPNFAEVIA
                     DHVTLAAGVRPDAALPAAVTGEIVGRIDDGAGVEALVVSIGCSTDRPGGGTYHITWSL
                     DKAGGREAKESNDVIARLGWEPVELPMPVTLRPARWP"
     gene            8888..9025
                     /locus_tag="VF589_10860"
     CDS             8888..9025
                     /locus_tag="VF589_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258123.1"
                     /translation="MDRDQAPNSDDLPQEDGKSDERAEQAKKELDEVPEPGTDPLHEG
                     P"
     gene            complement(9022..10461)
                     /gene="glpD"
                     /locus_tag="VF589_10865"
     CDS             complement(9022..10461)
                     /gene="glpD"
                     /locus_tag="VF589_10865"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX8258124.1"
                     /translation="MDSFDLLVVGGGINGAAIARDAARRGQKVLLVEKDDLAQHTSSS
                     SSKLIHGGLRYLEYYEFRLVREALQEREILLRTAPHVVRPLQFVLPHGEGMRPWWMVR
                     AGLRLYDLLAWGSSLPRSRGVGRRDEAARSPLQPGARRRLASYWDAWVDDARLVVLNA
                     LDAAEHGAEIATRTELLGARRLGEVWSAELSGVRRVEAKALVNAAGPWVAEVLGNRLG
                     ESSASAVRLVKGSHIVVPRLFVGDHGFILQQPDGRVVFALPYGEWTLIGTTDIPVETP
                     EQAVISEDEIAYLCEAANRYFVRQTGPEQLVWSYSGVRSLYDDGAADAQAVTRDYHLE
                     LDRDAGAKLLSVFGGKITTARALAAEALDKLGIEGRKQTAHAALPGGDIHPGFNAYLD
                     QLARWMPADLLLRLSRAYGTRLSEAIGGAGSMKELGRHFGAGLYEAEVAYLREREFAR
                     SAEDIMWRRTKLGLEMTAAQRTALDRYLR"
     gene            10564..12036
                     /locus_tag="VF589_10870"
     CDS             10564..12036
                     /locus_tag="VF589_10870"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HEX8258125.1"
                     /translation="MTEQALLVIDEGTTSTRAMLFAPDGACLGSEQSDLTQHYPASGL
                     VEDDAEEIWDKSLACARAMVEQAGGAERIAAIGIANQRETILFWSRRTGRALAPAIVW
                     QDRRTAGLCRKLKEEGHEHWVQARTGLLLDPYFSASKIGWALDHWPQLREAGDDLVVG
                     TVESWLVYRLTGGLHISDATNASRMALMDIEEGRWDDELLGLFGVPAASLPEIVDCAG
                     AYGVTTLFGAPIPICGMAGDQQAAAIGQACLAPGETKATYGTGAFVLVNSGATRPVSQ
                     HRLLATVAWQLGGKRSYALEGSIFVAGSLIQWLRDDLNLLASAAESEALARSVPDSGG
                     VQLVPALSGLGAPHWEPGARAAISGLSFATGRAHIARAALEAMAHQTRDLMLAFAADG
                     AAWGTLKIDGGMTCNDWLAQDLADMLGLTVERPDFVETTALGAAMLAGLGCGLFGSLE
                     EAASMRGDVRRFEPALAADARDARLAGWSKAVKAVLGANS"
     gene            12060..>13068
                     /gene="wecB"
                     /locus_tag="VF589_10875"
     CDS             12060..>13068
                     /gene="wecB"
                     /locus_tag="VF589_10875"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX8258126.1"
                     /translation="MTQRIHLVAAARPNFMKVAPLWHALAAAPDFDPVLIHTGQHYDP
                     NMSDAIFADLRLPQPDPHLGIGSGRHAEQTGGVMIAYERIAEADRPDWLVVVGDVNST
                     AACALVGAKLGIPVVHLEAGLRSRDRSMPEEIIRLVTDAIADVLWTPSPDADVNLADE
                     GIPAERVTCVGNIMLDSFELVRPAIAAAAVPAELGLAGRYGVVTLHRPSNVDDPAQLA
                     RLAEALVAAQAELPLVFPVHPRTAQRLAAGGLTGRLARAGVKLVDPLPYVRFMSLVTG
                     AAAAITDSGGIQEEATYLGIGSGSHAEQTGGVMIAYEKIAIADRPDWLVVVGDVNSTA
                     AC"
BASE COUNT         2064 a         4637 c         4317 g         2050 t
ORIGIN      
        1 cccagggcgg cgcgggcgcg gacacggtca atggcggcac cggcgacgac accctgtcct
       61 cgggcgcgat gtcgttcgac ggccgcggcg acctcaccct cgaggtcgat accttgattg
      121 gccagaacgg caatgaccgg atcgagatcg gcatgaagga cattgccgac ggcggcaacg
      181 acttcgacac ccttcagctc gacttcaccg ccagcaagct ggctgagaat ttcgtcttct
      241 cggccgacat gaccttcgcc aacggcggtt cggccaagaa tttcgaggcg ctgaccttct
      301 tcggcggccg cttcgccgac gtcgtcaccg gcggtgccaa tgccgacacc ctctatggcg
      361 gcggcggcaa cgatttgctc cgcggcgccc tcggcaacga catgatcgtc gacggtcagg
      421 gccgcgaccg catgttcggc gatgccgggg ccgacatctt ccgcaccagc ggcctcaacg
      481 ccgacgccga catctatgac ggcggcaccg acaaggacac tttgatgttc tgggacggcg
      541 aggatgacga ggaggtggaa tcgaccatgt cgggcttcct cgacctcgag aaccaggcgc
      601 tcaacgacgg cctcttgaag ggcgacacgt tcaagaacat cgaaaccttc atcggcgccg
      661 gccgcgacga cttcatgttc ggcaaccgcc ttgccaacac cttcatcggc ggcgacggcg
      721 atgacgtgct cgacggccgc gccggcaacg acctgctggt cggtggcgaa ggctccgaca
      781 ccctgaccgg cggcctcggc aaggacattt tcgacctgtc cgggcagggc aatcacggcg
      841 cctggttcgg cgacatcgtc accgatttca cccgcggcga ggacaagttc cagatctcgc
      901 tggaatcgct cggcctcgaa ggcgcccctg ccttcaccgt cgtggtcggc aacaacccgg
      961 cgccgaccgg cagcggcccg cagctgctgt tcgacaccgc caccgaccgg ctctggttcg
     1021 acctcgacgg cgccggcacc gaccatgaag gcatgctggt cgccaccctg gagggcgtca
     1081 ccaccctggc ggtgagcgac ttccagttcg tctaagcggc ggcgacgccc gcatctccag
     1141 ccccgggagc gcacccgcgc ttccggggct tttcttgtcc gccgcgcgcc cgccccggtt
     1201 ccaccgcgcc cgcgccgtct ctatatgctg gctaggccgg ccgtcgccgg cgtgagggag
     1261 caggacgtga atcgcgaaac ccgcatcgat gccggacggc cggaggagct ggatacatgg
     1321 accgtcgccg gccgcagctt ccgctcgcgg ctaattgtgg gcaccggcaa atataagagc
     1381 ttcgagcaga atgccgcggc ggtcgaggcc gcgggcgccg agatcgtcac cgtcgccgtc
     1441 cgccgcgtca atatcgccga ccccaagcag ccgatgctga ccgattatat cgatccgaag
     1501 cggatcacct atctgcccaa caccgccggc tgcttcaccg ccgaggaggc gatccgcacc
     1561 cttcgcctgg cgcgcgaggc gggcggctgg accctggtca agctcgaagt gctcggcgag
     1621 gccaagacgc tctaccccga catgatcgag acgctgcgcg cgaccgagat cctcgccaag
     1681 gaagggttcg agccgatggt ctattgcgcc gacgatccga tcgccgccag gaagctcgaa
     1741 gatgccggcg cggtcgcgat catgccgctg ggcgcgccga tcggctccgg cctcgggctc
     1801 cagaaccggg tgatgatccg catcatggtc gagcaggcgc gggtgcccgt gctggtcgac
     1861 gccggcgtcg gcaccgcatc ggatgccgcc gtggcgatgg agctgggctg cgacggcgtc
     1921 ctcatgaaca ccgccatcgc cgaggcgaag gagccggtgc tcatggcccg cgccatgaag
     1981 ctcgccgtcg aggccggccg cctcgcctac cgcgccggcc gcatggccaa gcgccgctac
     2041 gccgacccgt ccagcccgct ggcggggctg atctaggaag gccccgttcc ccggcgaaag
     2101 ccggggtcca gacgatctcg ccacctgggc cccggcttcc gccgaggaac gcgaggggcc
     2161 tcctgccacc gtcattccag cgcaagctgg aatctctctg cctttcttgc gacctcccaa
     2221 agaagtgaga tttccagctt gcgctggaat gacgaggcgc gctcgaactg gccccggcct
     2281 tcgccgggaa acatctaccg gccgccccag cccgaccagc ccggcccccg taccacccgc
     2341 ccgggcgtcg cgccggtatg cttgccgtcc ttcagcaccg gcaccccgtt caccagcacg
     2401 tgccggaccc cgaccgaata ttggtgcggc tgcttgaacg tcgccttgtc cgcgatggtt
     2461 ttgggatcga acaccaccac gtccgcgaaa tagcccggcg ccagccggcc gcgcccgcgc
     2521 agcttcagct gggcggcggg aagcgaggtc agccggcgga tcgcttccgg caacgggatc
     2581 accttctcgt cgcgcacata tttgccgagg aagcgggcga aggtgccgta ggcgcgcgga
     2641 tgcgggttgc tttcgaggaa gctgccctcc gccgccacgc ttccggaatc ggagccgagc
     2701 atggtccacg gccatttgat gttccgcgcg atattggcct cgtccatatg gaaaaaggcg
     2761 gcgccgaccc ggctgtcatc ctcgatgatg aggtcgatga tcgtgtcctc gggcgacgtg
     2821 ccgcgcagct tggcgacctc ggccagcgtc ttgccggtga gcggcttcaa cttgtcgttc
     2881 ttgaacgcga tcagcatcac gttctgcggc ccgccggcgt cgtgcagcag attttcccag
     2941 ccttcgggct cggcccgcat ctcgcggacc aggcgggcgc ggatggcggg gtccttgagc
     3001 cgcttcaccc aggcctcgtg gccgccctcc tgcacccaca acggcatggt cgcgtagagc
     3061 ccggtggcgc ccgccgtata cgtgtagatg ttggcggtga cgtcgatccc ttccgcccgc
     3121 gcctgccaca gcctcctcag catcggctcc gatttggccc aattctcgcg gcccgcgggc
     3181 ttcatatgat acatctggac gggcgccccg gtgcgccggc cgatggtcag caattcctcc
     3241 accgcctgct cgtagcgcga cgattcgctc ctgaggtgcg agatgtagcc gccgccatat
     3301 tcgtgcgccg cggcggtgag cgcgaccagt tcgtccgtgt cggcgaaatt gccgggcgcg
     3361 tagatcagcg acgatccaac gccgagcgcg ccctcgcgca tcgcctggcg gaccagatcc
     3421 tgcatccgct tcaactgctc cggcgtcgcc ttcttgttgt cggatccgat ttcgtggatg
     3481 cgcaccgtcg tcgcgcccac gaacgaggcg acgttgggcg agatgccctt cttttcgagg
     3541 tgctcgagat attggccgag cgtggtccag ggcacgtcga accggaagtc gctctggccc
     3601 ttcaccatgt ccgcccgcat cgtcggcgta atcggcccca tcgaccagcc ttcgcccatc
     3661 acttccagcg tcacgccctg ggtgatgtcg ctcatcgagc gcgggtcctg gagcagcgat
     3721 tcgttggccc agctcagcat gttgatgaag cccggcgcga ccgcgagccc gtccgccttc
     3781 acttcccggt ccgcggtcgc gccggaaagg tcgcccatcg cgaccacccg gtcgccttcg
     3841 atggcgacgt cgcccttgaa cggcacgccg ccctggccgt catagatcat gccgccgcgg
     3901 atgatggtgt cgtaatcggc cgcggcggcg gcgaccggcg cggcggcgag ggcgaagccg
     3961 gtcacggccg ccaaaagctt cctcagcaag ggcatcctcc tgttttgcgg gagactctag
     4021 cgccgccccc ggccggggcg caacgggcgg gcgtgggcca ggcctcttac gcatctaccg
     4081 tatctcagtt aggcatcgtc gaatgacaga gaaaccaaag tcccattcgc cgcacgcatg
     4141 gcagcccgat cacctcaggc ttgccgtcga ggccgccggc gtcgctctct gggcgtggaa
     4201 cgtggacaat gaccgcttca cgatggatga gcggggcttt gagctgtggg gcctgccctg
     4261 gcgggacacc gtcagcttcg aggaactctc ccagcatatc catccggccg atcgcgatcg
     4321 ggtgcgagcc gccttcgccg ctacacgggc ggtgctcggt ccttacgaaa ccgactttcg
     4381 gatcatagtc ggcgaggaga ttcggtggat cgccgcgcgc gggcagggcg aggatgtcgg
     4441 gatcgtcggc cgcaccatgt tcggcatctt cctcgacgtc accggtcgca agcaggccga
     4501 agagggtcac gagcttctcg ccggcgagat gagccaccgc gtgaagaacc tgctggcgat
     4561 tgcggccgga ctgacgcaga tcacctcccg cacgaccgcg tcgaccaagg acatggcccg
     4621 cgagctcatc cagaggctga ccgccttggg acgagcccat gatctggtga ggccgctgcc
     4681 gaagagccag ggatcggcgg cattgctcgg cgacctcctg tccgtgctcc tcgctcccta
     4741 cgacgacctt ggcgcgttca gcggccgaat acgcgtagcg gtcgagcgga tgggcgtcgg
     4801 cgaggccgcc gcgacgagcc tggccatggt cgtccacgag ctcgcgacca actccctgaa
     4861 atacggagca ttgtcgtccg acgtggggac gttggacatt tccactcgct ccaacggcga
     4921 caatatctgc ctgatatgga tggaaagggg cggccctccg gtcgaggccc cgccggaggc
     4981 ggtcggcttc ggcagcaagc tcgtcgagcg cagcgtctca agacacctcg gcggaaccat
     5041 cgagcacgat tggctggctg aagggctcgt ggccacgatc cagctcgatc gcgcgaagct
     5101 tagccattag aggcgccggg gcacgcccac gtcccaggat cgaacgaacc taggctctga
     5161 cccggctgca ccgttctgtg ctgatctcga tggcatccat gaactgaatg gtcatcatca
     5221 ggcggtgctc gtcatcctca atctcgatga aggaggacat gttgacgtgg cccatgttca
     5281 tgtccgcggc gaggatgctt cggaggctgg tgatcgcctc ctggcgggct gcggggaggt
     5341 cgggcaagac tttgccgccc gaatcctccg tgaagccgtt accgttgcag acatggaaat
     5401 agaaacgagg catggacata aagcgcgcct ggacctgctt cggttcctcc accggcagcg
     5461 gcgttgacgt tggagaaagt cttccgagat ggagcagaag cccagcaacg ggtcataatc
     5521 ggcttcagac gctggcgtac gaaatcctta ccttggccgc ggcctcggcc gccagccgcc
     5581 gcgcctcgtc ggtggtggcg ccggaggcga gcgccacggc catgcgccgg ttgggccgcg
     5641 tcaccggctt gccgaagatc cgcacgtcca cctcgccgcc ggcgccgccg aacgccaagg
     5701 cgtcggccag cccgccgatg ctgaaatgct ccgcctcccg gtccgccagg atcaccgccg
     5761 aggcgccggc cgcgtggcgg agcgcgacct cggggatcgg caggccgagg atggcgcggg
     5821 cgtggaggtc gaactcggac aggttctgcg agatcagggt gaccatgccg gtgtcgtgcg
     5881 gccgcggcga cagttcggag aagatcacct cttcgcccgc gacgaagaat tctacgccga
     5941 acaggccgta gccgccgaga tcgtcgacca ccttgcgcgc catcccctgc gcgcttgcca
     6001 gcgccgccgc ggacatggcc gcgggctgcc agctttccat atagtcgccg cgctcctggc
     6061 ggtggccgat cggcgggcag aacagcacgc cgtcgcgggt gcggatggtg aggagggtga
     6121 tctcatagtc gaagcggacg aactcctcga cgatcacccg cgcccggtcg ccgcgcatgt
     6181 tggcgacggc ataatgccac gccgcctcaa ggccagccgc gtcggcgacc gtgctctggc
     6241 ccttgccgga tgaggacatg accggcttga tcacgcacgg aaagccggtg tggccggcgg
     6301 cttcgcgaac ctcgtccagg ctttcggcga agcggaagcg cgaggtcctg agccccagcc
     6361 cgccggcggc gagctcgcgt atgccgtcgc ggttcatcgt catctgcgcc gcccgggcgg
     6421 aggggacgac gaccgtcccc gccgcttcga tctcggccag cgcctcggtc cggatcgcct
     6481 cgatctcggg gacgacataa tcgggattgt gcctcgccac cgccgcgcgc aggcgctcgc
     6541 cgtccagcat cgagaagact tcgctttcat cggcaacctg catcgccggc gcgccggcat
     6601 agctgtcgca ggcgatgaca tggcatccga gccgcttggc ggagatggtg aattcgcgcc
     6661 cgagctcgcc cgagccgagc agcaggatgc gtgcggttgc ggtcatcggc gctgcctccc
     6721 gggcgcggcg cgccggggcg gcgaggcggt gcctgcgttc gtcaaatcca tccctcccga
     6781 gctttccggc ggccttatag aacccgcggg ccgtgccgca atcgcggctt tccgacgaac
     6841 gtcggcgact ctggttcgtt ggcaggtgaa ccagaggaga tttccatgcc caagctcaag
     6901 cccttggccg aacaggtcat cgtcatcacc ggcgcctcca gcggcatcgg cctcgctacc
     6961 gcgaggatgg cggcggaggc cggcgcgcgg gtggtgctca gcgcccgcag ccgcgaggcg
     7021 ctcgcccagg cggtggacga gatcaagcag gccggcgggc aggcggcctt cgtcgccgcc
     7081 gacgtcaccc gccgcggcga gctcgaagcc gtcgcggccg aggcggtcga ccggttcggc
     7141 ggcttcgaca cctgggtcaa caatgccggc gtcggcatct ggggcctgat cgacgaggtc
     7201 agcgaggagg atatgcgcca gctgttcgag accaatttct ggggcatggt ctacggctcc
     7261 gagatcgcgg tccggcacct caaggcgaag ggcggggcgc tgatcaacgt gggcagcctg
     7321 acctccgacc gcgcctttcc ggtgcagggc atctattcgg cctccaagca tgccgtgaag
     7381 ggcttcaccg acgcgctgcg catggagctg gaggcggaga aggcgccgat ctcggtcacc
     7441 ctgatcaagc cggcgtcgat cggcacgccg atgccgcagc atgtgaaaaa ttattcggac
     7501 caggaagcca agttcccgcc gcccatctat gcgcccgaag aggtggcggc caccatcctt
     7561 cgcgccgccg agcggccgat ccgcgacgcc ttcatcggca gcggcgcgcg cacgatcagc
     7621 agcctgtcga gcaagacgcc cggcttcctc gactgggtca gcgccaagtt cctgatcccg
     7681 gcccaatatg gcgaccagcc ggcgactccg accgacaacc tccatcacgg ccaggccgaa
     7741 gcgaaggtga gaggcgacca tcagggcagc ctgatccggc ccagcgccta tacccgcgcg
     7801 gcgcgccatc cggcggccac gctggcgatc gccggcctcg ccgcggccgg gctcggcgcc
     7861 ctgttcgcgc ggcgccgcaa ccgcgaggag gagcagaagc tgctgcccgc gccgtcaggc
     7921 gtccgcgccg agcgggaata aaagcgccag ctcggccagc gtctcgtcca cgttgcggtg
     7981 gtggacgaac acgccgccgg cgccctccca caggtggcgg tacttgtcgt tgtcgtcgac
     8041 cagcacgtcg ccttcttcgg catggtcgcg cttgtcgcgc gccatgcagg tgatcatgcg
     8101 gacgccgggg aaatgctggg ccgcccagcg catcttctgg tccgccgccc aattgccgcg
     8161 ggggaggccg gtgaggatca cgggatcgag atgtctcacc gcctcgaaca gccgcgtcgc
     8221 ctccggcatc agcggcaggc tgaagtagaa atcggacgcc tgcgccagct tgctccagaa
     8281 gcggccgggc ccgcggcttt tctcataggc ctttggcttc atgccgagga tggcggtcgc
     8341 gcccgtgtcg aagtccgcga ggacgccgtc gcaatcgagg tagagccggc gcatgtcagg
     8401 gccagcgcgc cggcctcagc gtcaccggca tcggcaactc gaccggctcc cagccgagcc
     8461 gggcgatgac gtcgttgctc tccttggcct cgcgccctcc tgctttgtcg agcgaccagg
     8521 tgatatgata ggtgcccccg ccgggtcggt cggtgctgca cccgatgctc accaccagcg
     8581 cctcgacccc ggcgccgtcg tcgatccgtc ccacgatctc gccggtcacc gccgccggca
     8641 gcgccgcgtc gggccggacg ccggcggcca gggtgacatg gtcggcgata acctcggcga
     8701 aattaggccg gaactgcatc agcaactcct tgcgctgatc ctcgtccagc gcccagccga
     8761 tcacgccgct gcccttgcca gcctgatcgt gagcctgttg cggatcgagc gtcatggcga
     8821 tcagaaccgc tctggccggg agccgttccc cggctgcgcg cgttgccggc cagaaggaga
     8881 tatcatcatg gatcgcgacc aagccccgaa ctctgacgac ctcccgcagg aggacgggaa
     8941 gagcgacgag cgcgccgagc aggcaaagaa ggagctggat gaagtgcctg agcccggcac
     9001 cgatccgctg cacgaaggcc cttagcgcaa atagcggtcc agcgctgtcc gctgggccgc
     9061 cgtcatctca agccctaact tggtccggcg ccacattatg tcctcggcgc tgcgggcgaa
     9121 ctcccgctcg cggagatagg cgacttcggc ttcgtacagg ccggccccga aatgccgccc
     9181 gagctccttc atggacccgg cgccgccgat cgcctccgac agccgcgtgc cataggctcg
     9241 cgacaggcgc agcagcaggt ccgccggcat ccagcgcgcc agctggtcca gataggcgtt
     9301 gaagcccggg tggatgtcgc cgccgggcag cgcggcatgg gcggtctgtt tccggccctc
     9361 gatgccgagc ttgtcgagcg cttcggcggc caaagcgcgg gcagtcgtga tcttgccgcc
     9421 gaacaccgac agcagcttcg cgcccgcatc gcggtctagc tcgagatgat agtcgcgcgt
     9481 caccgcctgc gcgtcggcgg cgccgtcgtc gtagagcgag cgcacgcccg aatagctcca
     9541 caccagttgc tcgggcccgg tctgccggac gaagtagcgg ttcgcggcct cgcaaagata
     9601 ggcgatctcg tcctcgctga tcaccgcttg ctcgggcgtc tcgacgggta tgtcggtggt
     9661 gccgatgagc gtccactcgc cataggggag ggcgaagacg acccgcccgt cgggctgctg
     9721 gaggataaag ccatggtcgc cgacgaacag ccgcggcacg acgatatggc tgcccttgac
     9781 cagccgcacc gcgctggcgc tgctctcgcc gagcctgttg cccagcacct ccgccaccca
     9841 cggcccggcg gcgttgacca gggccttggc ctcaacccgc cgcacgcccg aaagctcggc
     9901 gctccagacc tcacctaggc ggcgcgcacc gagcaactcg gtgcgggtgg cgatctcggc
     9961 gccatgctcg gccgcatcga gcgcgttcaa gacgaccagc cgcgcatcgt cgacccaggc
    10021 atcccaatag ctggcgagcc gtcgccgcgc gcccggctgg agcggcgacc gcgccgcctc
    10081 gtcgcgccgg ccgacgccgc gcgagcgggg caggctgctc ccccaggcga gcaggtcgta
    10141 gagccgcagc cccgcccgga ccatccacca cggccgcatc ccctcgccgt gcggcagcac
    10201 gaattgcagc ggccgcacca cgtgcggcgc cgtgcgcaac aatatctccc gctcctggag
    10261 cgcctcgcgc accaaccgga actcataata ttcgaggtag cgcagcccgc catggatgag
    10321 cttcgacgag gatgagctgg tgtgctgggc aaggtcgtcc ttctcgacca gcagcacctt
    10381 ctgcccccgc cgcgccgcgt cgcgcgcgat cgccgctccg ttgatgccgc cgccgacgac
    10441 gaggagatcg aagctgtcca cggaattgcc ttacgtgatt ccagcggaag ctggaatccg
    10501 ggcaatgttc gtgcgaccaa tgccgctgtg gatcccggct atggccggca cgacggaggc
    10561 gaggtgaccg aacaagccct gctcgtcatc gacgaaggca cgaccagcac gcgcgccatg
    10621 ctgttcgcgc cggacggggc ctgcctcggc tccgagcaaa gcgacctcac tcaacattat
    10681 ccggcctccg gcctggtcga ggatgacgcc gaggaaattt gggacaagag cctcgcctgc
    10741 gcccgggcga tggtggagca agcgggcggc gcggagcgga tcgcggcgat cggcatcgcc
    10801 aaccagcgcg agacgatcct gttctggtcg cggcggacgg ggagggcgct ggccccggcg
    10861 atcgtctggc aggatcgccg caccgccggt ctctgccgaa agctgaagga ggaggggcac
    10921 gagcactggg tgcaggccag aaccggcctg ctgctcgacc cctatttcag cgccagcaag
    10981 atcggctggg cgctcgatca ttggccgcag ctccgggaag ccggcgacga cctcgtggtg
    11041 ggcaccgtcg aaagctggct cgtctaccgc ctcaccggcg gcctccacat ttccgacgcg
    11101 accaacgcct cgcgcatggc gctgatggac atcgaggagg gccgctggga cgacgagctg
    11161 ctcggcctgt tcggagtgcc cgcggcgtcg ctcccggaga tcgtggactg cgcgggcgct
    11221 tacggcgtca ccacgttgtt cggcgctccg attccgattt gcggcatggc cggcgaccag
    11281 caggcggctg ccatcggcca ggcctgcctc gcgccgggcg agaccaaggc gacctatggc
    11341 accggcgcct tcgtcctcgt caatagcggc gcgacgaggc cggtttcgca gcaccggctg
    11401 ctggccaccg tcgcctggca gctgggcggg aagcgcagct acgccctgga aggttcgatc
    11461 ttcgtggccg gcagcctgat ccagtggctg cgcgacgacc tcaaccttct tgccagtgcc
    11521 gccgaaagcg aggcgctcgc ccgctcggtg ccggacagcg gcggcgtcca gctggtcccg
    11581 gcgctctccg gcctcggcgc gccgcattgg gagcccggcg cccgcgccgc catctccggc
    11641 ctcagcttcg ccaccggccg cgcccacatc gcccgcgccg cgctcgaggc gatggcgcac
    11701 cagacccgcg atctgatgct cgccttcgcc gccgacggcg ccgcctgggg caccctcaaa
    11761 atcgatggcg gcatgacgtg caacgactgg ctggcccagg atctggcgga catgctcggc
    11821 ctcaccgtcg agcggccgga cttcgtcgag actaccgcgc tcggcgccgc gatgctggcc
    11881 gggctcggct gcggcctgtt cggctcgctg gaggaagcgg cctcgatgcg cggcgacgtg
    11941 cggcggttcg agcccgccct cgcggccgat gcccgggacg cgcggctcgc cggctggagc
    12001 aaggccgtca aagccgtgct gggagccaat tcttaagcct ctccggctat gctcccgcca
    12061 tgacccagcg catccatctt gtcgccgccg cgcgccccaa tttcatgaag gtcgcgccgc
    12121 tctggcacgc gctcgccgcc gccccggatt tcgacccggt gctgatccat accggccagc
    12181 attacgatcc caacatgtcg gatgcgatct tcgccgatct ccgtctgccg cagccggacc
    12241 ctcatctcgg catcggatcg ggccgccatg ccgagcagac cggcggcgtg atgatcgcct
    12301 atgaacggat cgccgaggcg gaccggcccg actggctggt cgtggtcggc gacgtcaatt
    12361 ccaccgccgc ctgcgctttg gtcggcgcca agctcggcat ccccgtcgtc catctcgaag
    12421 cgggcctgcg cagccgcgac cgctccatgc ccgaggagat catccgcctc gtcaccgacg
    12481 ccatcgccga cgtgctctgg acgccgtcgc ccgacgccga cgtcaacctc gccgacgaag
    12541 gcatccccgc cgagcgcgtc acctgcgtcg gcaacatcat gctcgacagc ttcgagctcg
    12601 tccgccccgc catcgccgca gccgcggttc cggctgagct cggtctggcc gggcgctacg
    12661 gcgtggtgac gctccaccgc ccgtccaacg tcgacgatcc ggcccagctc gccaggctcg
    12721 ccgaggcgct cgtcgcggct caggccgagc ttcctctggt cttcccggtc catccccgca
    12781 ccgcccagcg gctcgccgcc ggcgggctca ccggccggct cgcgcgcgcc ggggtaaagc
    12841 tggtcgatcc gctgccttat gtccgcttca tgagcctggt cacgggggcc gcggctgcca
    12901 tcaccgacag cggcggcata caggaggagg cgacctatct cggcatcggc tcgggaagcc
    12961 atgccgagca gaccggcggg gtgatgatcg cctatgagaa gatcgcgatc gccgaccgcc
    13021 cggactggct ggtcgtggtc ggagacgtca attccaccgc cgcctgcg
//
LOCUS       DASVRK010000269         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_548364, whole genome shotgun sequence.
ACCESSION   DASVRK010000269 DASVRK010000000
VERSION     DASVRK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548364"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VF589_10880"
     CDS             <1..142
                     /locus_tag="VF589_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8258127.1"
                     /translation="DVRDPKAAVTAFQRRFRPEHVDGVIEGECRAILLSLLLDRERGQ
                     AR"
     gene            149..571
                     /gene="rnpB"
                     /locus_tag="VF589_10885"
     ncRNA           149..571
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF589_10885"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(613..843)
                     /locus_tag="VF589_10890"
     CDS             complement(613..843)
                     /locus_tag="VF589_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8258128.1"
                     /translation="MATSPQRPDGWYGLGFWIGGQGPTRNWGHGGGAPGMNAAFRVYP
                     ELDATVVALANLDPLAADNQSDFYANRMALAE"
     gene            1013..>2533
                     /locus_tag="VF589_10895"
     CDS             1013..>2533
                     /locus_tag="VF589_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX8258129.1"
                     /translation="MIELSRTAAALIAAFCGFWLCLAVWAVISARRRSRTAFGHSAAA
                     ERCEAMLGAGPAIPLVAFPDGSLEAPARLAGMFGMASPPTHISELSALGAGLTADTSS
                     LHEEVRTASASGGDFSLAVRAEGSARVLLVRGSPAPPPYPPGSVLLWFIDATDSEEQM
                     AALRGEVFRLTGALGALSALIEAAPFPVWHRGPDLRLAMVNSAYVAAVEAEDAMEVIE
                     GGVELVDESEGRSPLSQAAAVRDKGAPAARTVPATIAGERRMMRVVEVPLGKSGVGGY
                     AIDVEDREQARADLARFVRAQRDMLDRLSAGVAQFSRDRHLIFFNQPFARLFALKGDF
                     LADQPEFNRVIDAMREGGHLPEVRDFPDWKEGRRRWFTSGLAADEEDWLLPGGKHLRV
                     VAQPLPDGGLLLIFEDRTEQIQLASARDTLLRVRTATFDNLFEAVGVFASDGRLHLWN
                     NRFRQVWQFEEEQLAAHPRVDAMTPHIATRLKNPNHAGLVRELVRSATVERKQRTGR"
BASE COUNT          352 a          909 c          889 g          383 t
ORIGIN      
        1 tgacgtgcgc gaccccaaag ctgccgtcac cgcgtttcag cgccgcttcc ggccggagca
       61 tgtcgacggc gtcatcgagg gcgagtgccg ggcgatcctg ctttcgctgc tcttggatcg
      121 cgagcgggga caggctagat agcccgcgcc agagggtcgg gcggccgctg tgcgacgctt
      181 cggcggagca gagaggaaag tccgggctcc acggaacgac ggtgccggct aacggccggc
      241 gggccgggaa ttcgttcccg gctcagggaa agtgccacag aaagtaggtc gcgctggctt
      301 cggcccaggt cttcggaagc gtgacggtga aagggtgggg taagagccca ccgcgggccc
      361 ggcaacggga acggcacggc aaaccccacc gggagcaaga ccgaataggg gcggcgaggg
      421 gcatatgccc cggggcccgt tccggcccag tcgcccgggt tggttgcttg agggagggag
      481 cgatccctct cctagaggaa tggtcgccca gtcctccgaa gccttggcgg aggaggatgg
      541 acagaacccg gcttacagac cctctggcat ttttccttcg cagacgggcg gagggcggcg
      601 gcggcggatc gcctattcgg cgagcgccat ccggttggcg tagaagtcgg actggttgtc
      661 ggcggcgagc gggtcgaggt tcgccagggc gacgacggtc gcatcgagct cggggtagac
      721 gcgaaacgcc gcgttcatcc ccggcgcgcc cccgccatgg ccccagttgc gggtcggtcc
      781 ctggccgccg atccagaagc cgagcccgta ccagccgtcc ggccgctgcg gcgacgtcgc
      841 catcctgaac tcgcccgcgg ccatggcgca gcatggcacc ttcgcgccgc cgccgccggc
      901 ttatgcctcg ccgcgcgtgt cattccgctc acccacttcc gccgccgctc cactgtggct
      961 agacatcggc catgcattct cgcctcaccg tccgaagggc tccgcaccgg agatgatcga
     1021 gctgtcgcgc accgcggcgg cgctgatcgc cgctttctgc ggtttctggc tgtgccttgc
     1081 ggtctgggcg gtgatatcgg cccgccgccg cagccgcacc gccttcggcc attcggcggc
     1141 ggccgagcgc tgcgaggcga tgctcggcgc cggaccggcc attccgctcg tcgctttccc
     1201 cgacggcagc ctcgaagccc cggcgcggct ggccggcatg tttggcatgg cgagcccgcc
     1261 gacgcacata tccgaacttt cggcgctcgg cgccggcctc accgccgaca cctcgagcct
     1321 gcacgaggag gtgcgcacgg cctcggcctc gggcggcgat ttctcgctgg cggtgcgggc
     1381 ggaaggctcg gcgcgcgtgc tgctggtgcg cggctcgccg gcgccgccgc cttatccgcc
     1441 gggcagcgtc ctgctctggt tcatcgatgc caccgacagc gaggagcaga tggcggcgct
     1501 gcgcggcgag gtgttccgcc tcaccggcgc gctcggcgcg ctctcggcgc tgatcgaggc
     1561 ggcgccgttc ccggtctggc atcgcggtcc cgaccttcgc ctcgccatgg tcaacagcgc
     1621 ctatgtcgcc gccgtcgagg ccgaggacgc gatggaggtg atcgaaggcg gcgtcgagct
     1681 ggtcgacgaa agcgagggcc gctcgccgct gagccaggcc gcggccgtgc gcgacaaggg
     1741 cgcgcccgcc gcccgcaccg ttcccgccac catcgccggc gagcgccgga tgatgcgggt
     1801 ggtcgaagtg ccgctcggca agtccggcgt cggcggctac gcgatcgacg tcgaagaccg
     1861 cgagcaggcc cgtgccgacc ttgcccgctt cgtgcgcgcc cagcgcgaca tgctcgaccg
     1921 cctctcggcg ggcgtcgccc agttcagccg cgaccgccac ctgatcttct tcaaccagcc
     1981 gttcgcgcgg ctgttcgcct tgaagggcga tttcctcgcc gatcagccgg agttcaaccg
     2041 ggtgatcgac gccatgcgcg agggcggcca tctgccggaa gtgcgcgact ttccggactg
     2101 gaaggagggg cgccggcgct ggttcaccag cggcctcgcc gccgacgagg aggattggct
     2161 gctccccggc ggcaagcatc tgcgcgtcgt cgcgcagccg ctgcccgacg gcggcctgct
     2221 gctcatcttc gaggaccgca ccgagcagat ccagttggcc tcggcgcgcg acaccttgct
     2281 gcgcgtccgc accgccacct tcgacaacct cttcgaagcg gtcggcgtat tcgcctccga
     2341 cggccgcctg cacctgtgga acaaccgctt ccggcaggtc tggcagttcg aggaggagca
     2401 gctcgccgcc catccgcgcg tcgatgcgat gacgccgcac atcgccaccc gcctcaagaa
     2461 cccgaaccat gcgggcctcg tccgggagct tgtccgcagc gccactgtcg agcgcaagca
     2521 gcgcaccggc cgc
//
LOCUS       DASVRK010000270         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_429986, whole genome shotgun sequence.
ACCESSION   DASVRK010000270 DASVRK010000000
VERSION     DASVRK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429986"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF589_10900"
     CDS             complement(<1..224)
                     /locus_tag="VF589_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258130.1"
                     /translation="MDQQLERVSRRMLLGGLGGGAIAIGALAAPGLPLSLLDTREGAQ
                     LPNWWDRQYWSLESAGITQWQRQTGLLFYM"
     gene            372..1121
                     /gene="otsB"
                     /locus_tag="VF589_10905"
     CDS             372..1121
                     /gene="otsB"
                     /locus_tag="VF589_10905"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX8258131.1"
                     /translation="MDVPLPSPPSDLVTGAALFLDFDGTLVELAESPAAIMVGPGVPS
                     LLSRLDAALSGRLAVVSGRALADLESHLDCTGLAVSGSHGLELRLRDGARIPLAAPGR
                     LAEAGEAIESFAAERPGLLVEAKPFGTAVHYRAAPELGGEVESFLSGLAARLGLAVQH
                     GKMVVELRPHGADKGDALRAFMAEPPFTGARPLFVGDDLTDEAAFAAAAALGGGGILV
                     GPPRPTAARWRLAGVPETIAWLGEAAGGADG"
     gene            1114..>1955
                     /locus_tag="VF589_10910"
     CDS             1114..>1955
                     /locus_tag="VF589_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8258132.1"
                     /translation="MADLSLWPIGNCQVSALVDAAGSFVWGCQPRVDGDPLFCSLLEP
                     RAVGGHPRGVWRVAIENQVAVEQRYLRNTPILVTRLTDADGAAAEIFDFCPRFERTGR
                     MYRPVAFARIVRPVTGAPRLRMILNPAADWGAREAERTSGTNHIRYLLKPQPLRLTTD
                     APIGPIAEGRAFRLERPLHFFLGPDEPFVGHVGHSLATMLHQTSDHWREWVRGLAVPL
                     EWQRQVIRAAITLKLCQHEETGAIVAALTTSIPEAPGSGRNWDYRYCWIRDAYYTVQA
                     LNRLG"
BASE COUNT          254 a          721 c          671 g          309 t
ORIGIN      
        1 atcatgtaga agagcaggcc ggtctggcgc tgccactggg tgatgccggc gctttccagc
       61 gaccaatatt ggcggtccca ccaattgggc agctgggccc cttcccgggt gtcgagcagg
      121 ctgagcggca ggccgggcgc cgccagtgcc ccgatcgcga tggctccccc gccgaggccg
      181 cccaaaagca tgcgccgcga gacgcgttct aattgctggt ccattccccc accttccccc
      241 aaaagtgcgt gccgcggacg ataatggaaa agcggcggcg gcgatacctg ccggccgact
      301 ttatttcgcg acatttggcg ccggcgccgc cggtgcttga gattccgccg gggagccgcc
      361 acgggagcgg gatggatgtc ccgcttccgt cgccgccttc ggacctcgtc acgggcgcgg
      421 ccttgttcct cgatttcgac ggcaccctgg tcgagcttgc cgagtcgccc gccgccatca
      481 tggtcgggcc gggcgtgcca agcttgctca gccggctcga tgcggcgctt tcgggcaggc
      541 tcgcggtcgt cagcggccgg gcgttggcgg acctcgagtc gcacctcgat tgtaccggcc
      601 tcgccgtctc gggctcgcat gggctcgagc tgcgcctgcg cgacggcgcg cgcattccgc
      661 tcgctgcccc gggccgcctc gccgaggcgg gcgaggccat cgaaagcttt gcagccgagc
      721 gccccggcct gctggtcgag gccaagccgt tcggcacggc cgtccattat cgcgccgctc
      781 cggagctcgg cggcgaggtc gaaagcttcc tctccggcct cgccgcccgc ctcggcctcg
      841 ccgtccagca cggcaagatg gtggtggagc tgcggccgca cggcgccgac aagggcgatg
      901 ccttgcgtgc cttcatggcg gaacctccct tcaccggcgc gcgtcccttg ttcgtgggcg
      961 acgatctgac cgacgaggcg gcctttgcgg cggcggcggc gctcggcggc ggcggcatcc
     1021 tggtggggcc gccgcggccg acggcggcgc gatggcggct ggccggtgtc cctgagacga
     1081 tcgcgtggct cggcgaagcg gcgggcggcg ccgatggctg acctttcgct ctggccgatc
     1141 ggcaattgcc aggtgagcgc gctggtcgac gccgcgggca gcttcgtctg gggctgccag
     1201 ccccgcgtcg acggcgatcc gctcttctgc tcgctgctgg agccgcgcgc cgtaggcggc
     1261 cacccgcgag gcgtatggcg cgtggcgatc gagaaccagg tcgcggtcga gcaacgctat
     1321 ctgcgcaaca cgccgatcct cgtcacccgc ctcaccgacg ccgacggcgc cgcggccgag
     1381 atattcgact tctgcccgcg cttcgagcgc accggccgca tgtaccgccc ggtcgccttc
     1441 gcgcggatcg tgcggccggt gaccggcgcg ccgcggctgc gcatgatcct caacccggcc
     1501 gcagactggg gcgcgcgcga ggccgagcgg accagcggca ccaaccacat acgctatctc
     1561 ctgaagccgc agccgctcag gctcacgact gacgcgccga tcggtcctat cgccgagggc
     1621 cgcgccttcc ggctcgaacg gccgctccac ttcttcctcg gccccgacga gccgttcgtc
     1681 ggccatgtcg gtcacagcct ggcgacgatg ctccaccaga cctccgacca ttggcgcgaa
     1741 tgggtgcgcg ggctcgccgt gccgctcgaa tggcagcgcc aggtgatccg cgccgccatc
     1801 accctcaagc tttgccagca tgaggagacc ggcgcgatcg tcgccgcgct caccacctcg
     1861 atccccgagg cgcccggcag cggccgcaac tgggactatc gctattgctg gatccgcgac
     1921 gcttattaca ccgtgcaggc gctgaaccgg ctcgg
//
LOCUS       DASVRK010000271        11843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_430091, whole genome shotgun sequence.
ACCESSION   DASVRK010000271 DASVRK010000000
VERSION     DASVRK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11843
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430091"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..370
                     /locus_tag="VF589_10915"
     CDS             56..370
                     /locus_tag="VF589_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8258133.1"
                     /translation="MFQSAITGSIDNRIEPRAEVYHRTRATMPDRQVCSVLVVNISAS
                     GLMLRSDALVAVGDRIQVMLPTAGEVAATVRWALGGRLGCQFDAAIPGPRYAAVLAKL
                     DR"
     gene            complement(371..2428)
                     /gene="recG"
                     /locus_tag="VF589_10920"
     CDS             complement(371..2428)
                     /gene="recG"
                     /locus_tag="VF589_10920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX8258134.1"
                     /translation="MRPDILNPLFAEVEVLKGIGPALAKPLKRLGLERVVDILFHLPV
                     SWIERKRVDILDMADAGSVVTVTVTPVDYRQAGPRSPFRVLAADRDGNYLTLTYFNNP
                     GWAKKQLQLGVPRVVSGRMEMYGQELQIVHPDYVLPPEEAATLPEREPVYPLSEGLTN
                     NRLGQLAGQALARVPELGEWIEPSLLDRRQWPGWSDALQTAHRARNNSKARERLAYDE
                     IFANQLALRLVRASSRKRRGVPLRGDGRLRGALKLPYAPTGAQARAIAEIDGDLQQEV
                     PMLRLLQGDVGSGKTLVALMALLSAVEAGAQGALLAPTEILARQHHDTLSRQLAGLPV
                     NVAILTGREKGRAREATLMGLADGSIHILVGTHAIFQQAVSYRRLGLAVVDEQHRFGV
                     AQRMMLAQKAERPPHLLVMTATPIPRTLTLTNYGEMDVSRLDEMPPGRQPIETRVLSV
                     ERLPEVVDALARHIARGAQSYWVCPLVEESEASDQAAAEERAQVLRLRFGDKVGLVHG
                     RMKGPEKDAVMAAFQRGDIAVLVATTVIEVGVDVPNATLMIVEGADRFGLAQLHQLRG
                     RVGRGDQRSVCLLLRGNALSETGRARLALMRETNDGFRIAEEDLRLRGAGEMLGTRQS
                     GEAAFRIATPDLLHDLLQCAQDDARLLIDRDGGLAGPRGQAARTLLYLFERDAGVALL
                     RSG"
     gene            complement(2483..3436)
                     /locus_tag="VF589_10925"
     CDS             complement(2483..3436)
                     /locus_tag="VF589_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258135.1"
                     /translation="MLMRPSPAARPVPAEALFPTWRGREPYADRIKPATAALAERHPG
                     LAAALAALPFDRFEDAWASDAVIRKYNEVPVEAAEMMAAIQSDLRAPWLCALMLWHIG
                     RFEEVFAATGLDEEFALHYADGFHRILDQIEADPAFADLAKDSFLKDLWLARVVMIPA
                     FAQVWWPHSGLPAKTVLRAGSAAAAHVFLRCGGRRPFLEGHTHDPVAKAYWNEAGWGE
                     ALRLAALALPALPEARGVFGTAWFYDPEVKRVTPRLSFAQDLQIGRGGFRMLVGSNEG
                     AIANATATSASRRELYDKGEYLPTDYAIIWSRVDLIHAYGA"
     gene            complement(3471..4445)
                     /locus_tag="VF589_10930"
     CDS             complement(3471..4445)
                     /locus_tag="VF589_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8258136.1"
                     /translation="MSLKRRILAAASSAGLAAAARKATAGQLRILCYHGLWVTPGYEF
                     GDCTFISPEQFEARMARLKRSGRPVLPLAEAVDRLARGTLPEAAVVITIDDGWVSTFT
                     HMLPVLEAYALPATLYATTWYAGRALPVVNVAVDYLRLAGGRPDIDPVAKTAAIEALP
                     LDDRLAALRRFGAELGVDEAWLDLQQFNIMSADQFAEAKRRGLDVQLHTHRHIDIATR
                     IDTLAAEVEENRAFLRAAIGDRPFTHFCHPSGSNHPRARALLAAAGVHSATLVTEGLN
                     APGADPLALRRLLDGRRVSDAEFDAYLSGLLHYTAKLRAAARLKPASR"
     gene            4647..4913
                     /locus_tag="VF589_10935"
     CDS             4647..4913
                     /locus_tag="VF589_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX8258137.1"
                     /translation="MDPADRIKRLRYRAWHRGTREADFLIGGYFDRHHGGWGEAETAW
                     FEALIEEQDVDIMAWAIGTAEPPARYNGGMMRRLQTLDYIKHPE"
     gene            5015..8461
                     /gene="mfd"
                     /locus_tag="VF589_10940"
     CDS             5015..8461
                     /gene="mfd"
                     /locus_tag="VF589_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX8258138.1"
                     /translation="MLRAKEPVTLAGAPAGFVPWLAADLARAAAGTGGGARAVFIASD
                     EAAMRHILDAAPYIAPELETLSFPAWDCLPYDRSSPSLRSTSERLATLHALQRKPAKP
                     QLLVTTVNAATQLTLSPFRIRQLVARLAPGERIDLDRLAALLSANGYMRTDTVQDAGE
                     FAVRGGIVDLFPAGEEQGLRLDFFGDEIESVRRFDPSTQRTTGTAEGGFTLLPASETL
                     LDEGSVKRFRSRYRELFGATATGDPVYQAVSEGRRLAGVDHWLPLFEERLATLFDHLG
                     ERDIIVRDAGHAGAAEARFEAVADYYENRVRAQSGDAGSYRPLKPETLYLQPGDWEAL
                     IAERPLHIVSPYREPPGPAVADFGVDGPRDFAPERAQNVNVYEAVVEHVTALRRAGKK
                     VVLASYSRGARERLKGLLEDHGLKKLALADGWQEALGGPVALTVIQLDHGFTTADIAL
                     LTEQDMLGDRLVRRRKRKKSQDAFLNELAALSAGDLVVHADHGIGRYEGLTQIPVGSS
                     PHDCVALEYAGGDKLYVPVENIDVLSRYGSESDGVALDRLGGEAWQRRKSRMKERIRE
                     IAGELIKTAAARALRPGQVAEPDTGYAAFADRFPYEETDDQERAITDVIADLGAGRPM
                     DRLVCGDVGFGKTEVALRAAFIAAMSGLQVALVCPTTLLARQHFNTFRERFQGFPLEI
                     GRLSRLVPAAEAKATKEGIKDGKIDIVIGTHAILAKSVEFKQLGLVIVDEEQRFGVTH
                     KERLKAMKADVHVLTLTATPIPRTLQMAMSGLRELSVIQTPPVDRLAVRTYVMPWDPV
                     VLREALLREHYRGGQSYFVAPRIADLPDLEEFLRSEVPEVSFIVAHGQMSPTEVEERM
                     SAFYDRQYDVLLSTTIVESGLDIPSANTLIIHRADRFGLAQLYQLRGRVGRAKTRAYA
                     YFTTPPTRTVTEGADKRLQVLANLDTLGAGFQLATHDLDIRGAGNLLGDEQSGHIKEV
                     GFELYQSMLEEAILEARAAPGAPVRAGDFSPQITVDAPIMLPEEYVPDLDLRMGLYRR
                     MNELEGGQEIESFAAELIDRFGKLPQATDNLLKVIEVKLNCRKAQVVKLDVGPKGALV
                     HFHNDTFPDLEGLIAYVQRLKGTAKLRPDSKLVISRSWPDPQSRVNGALQLSRGLAKI
                     IG"
     gene            complement(8451..9632)
                     /locus_tag="VF589_10945"
     CDS             complement(8451..9632)
                     /locus_tag="VF589_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX8258139.1"
                     /translation="MQPPIERLSLPQARRVALAAQGFGLPRPAEVDRGHLRRIADRLS
                     LHQIDSVNVLARAHYLPAFSRLGGYDRGLIDRAAWGPLGERRLFEYWAHEASLLPVAL
                     HPLLRWRMARADRGERGWKSLRAFAGERRAEAEAVLARIRAEGPLAASDFDGARSRSG
                     WWEWGAAKRALEWLFMAGHVTTATRRRSFERVYDLTKRVIPPAILALPTPDESEAHRR
                     LIDRAARALGVATATDLRDYFRLAPEPARAAIAELAEAGMLLPVAVDGWGKPAFLHRE
                     ARRPRRIEGQALLVPFDPLVWERARTERLFGFRYRIEIYTPADRRVHGYYVLPFLMDE
                     ALVARVDLKADRQRSRLMVQAVTFEPGAPAETAERLDAELERMAAWLQLEAVERRAVS
                     R"
     gene            complement(9641..9994)
                     /locus_tag="VF589_10950"
     CDS             complement(9641..9994)
                     /locus_tag="VF589_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258140.1"
                     /translation="MLWHDFKALLGALGLSEQALHIYGGVIVYALALKLSRRPPADLL
                     PLVVVLFAALLNEGQDLKNAVDWRKTLELTEGIGDVVNTILLPACYTFWSRHRRRREA
                     SPPPPEPAPVHASPP"
     gene            complement(10093..10491)
                     /locus_tag="VF589_10955"
     CDS             complement(10093..10491)
                     /locus_tag="VF589_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258141.1"
                     /translation="MTKPNLNLSGLWLGSFAYPAGQGPTTPFLARIEDHDGSLGGTII
                     EPNTIGATSDTLEAILSGSRHGHAVDFTKAYDGASDAAHAVDYVGRLSGDGTSVTGVW
                     SLGTLDGSFEMHREAVWEESVGREAEVIKL"
     gene            complement(10534..>11843)
                     /locus_tag="VF589_10960"
     CDS             complement(10534..>11843)
                     /locus_tag="VF589_10960"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX8258142.1"
                     /translation="IGAGDSGATQSRDPMTGVRDARAARAWIEEQLLGAAGPEPHLVL
                     LLLAVSRFDTINAAFGRATGDAVLQAAARRIERLVDADGRRRFVARLAGAEFAVLLAP
                     PASLAEGRFLAGQIVEGIGRPFMSGDHVITLGSRVGVAASGPGDDAAALLRRASEALV
                     DAKGGEAGPIRVFDAGAQSDSARGDRLEIDLRRALDQDEIEILFQPQVSITNGSIVGA
                     EALARWRHPDFGELGAVTLFNVAERSDYLVQLSDHVQRKAIAAASAWPASLGALRLAV
                     NITADDIVRPGFAAQFLALVEDSGFDPRRLTVEVTESGLIEDLNAAASLLARLREGGL
                     RIAIDDFGTGYSSLAYLKALPLDYLKIDKRLSEDITGSPRDRIVVRSVIDMARSLGLD
                     VIAEGVETEEQLGLLAQEGCTLYQGFLCSPPIDTAALARLVAG"
BASE COUNT         1853 a         3966 c         4307 g         1717 t
ORIGIN      
        1 ctaatccttg ccgcgacgac cgacgcgatg ccgcggccgg tcagcggaga agagcgtgtt
       61 tcagtccgca atcacgggat cgatcgataa ccgcatcgag ccacgcgcgg aggtgtatca
      121 ccgcacccgc gcgacgatgc ccgatcggca ggtctgctcg gtgctcgtcg tcaacatctc
      181 cgcgagcggg ctgatgctgc gcagcgacgc gctggtcgcg gtcggcgacc gtatccaggt
      241 tatgctgccg accgcgggcg aggtcgccgc aacggtacgc tgggcgctcg gcggacgcct
      301 cggctgccag ttcgacgcgg cgatcccggg gccgcgctac gctgcggtgc tcgccaaact
      361 ggatcgctga tcaaccggaa cgcagcagcg ccacgcccgc atcgcgttcg aacagataca
      421 gcaacgtccg cgccgcctgc ccgcgcggcc ccgcgagccc gccgtcgcgg tcgatcagca
      481 gccgcgcatc atcctgcgcg cattgcagca gatcgtggag caaatcgggg gtcgcgatcc
      541 ggaacgccgc ctcgcccgac tgccgcgtgc ccagcatctc gcccgcgccg cgcagccgta
      601 gatcctcttc ggcgatgcgg aagccgtcat tggtctcgcg catcaaggcc aagcgggcgc
      661 ggccggtctc gctcagcgca ttgccgcgca ggagcaggca gaccgagcgc tggtcgccgc
      721 ggccgacccg gccccgcaat tggtggagct gggcgaggcc gaagcggtcg gcgccctcga
      781 cgatcatcag cgtcgcgttg gggacatcga cgccgacttc gattaccgtg gtcgcgacca
      841 gcaccgcaat gtcgccgcgc tggaaggcgg ccatcaccgc gtccttctcc ggccctttca
      901 tgcggccatg gacgagcccg accttgtcgc cgaagcgaag ccgcagcacc tgcgcccgct
      961 cttccgcggc ggcctggtcg gaagcctcgc tctcttcgac caaggggcag acccaataag
     1021 actgggcgcc gcgggcgatg tggcgggcga gcgcgtcgac cacttcgggg agccgctcta
     1081 cgctcagcac ccgggtctcg atcggctggc ggccgggcgg catctcgtcg aggcggctga
     1141 cgtccatctc gccataattg gtgagggtga gggtgcgggg gatcggagtc gcggtcatga
     1201 ccagcaggtg cggcggccgc tcggctttct gggcgagcat catgcgctgg gcgacgccga
     1261 agcgatgctg ctcgtcgacc acggcgaggc cgagccggcg gtagcttacc gcctgctgaa
     1321 agatggcgtg ggtgccgacg aggatgtgga tcgagccgtc ggcgaggccc atcagggtgg
     1381 cctcgcgcgc cctccccttc tcgcgcccgg tgaggatggc gacgttgacc ggcaggccgg
     1441 cgagctggcg cgagagcgtg tcgtgatgct ggcgcgcgag gatctcggtg ggggcgagca
     1501 aggcgccttg cgcgcccgct tcgaccgcgc tcagcagcgc catcagcgcg accagggtct
     1561 tgcccgagcc gacatcgccc tggagcagac ggagcatggg cacctcctgc tggaggtcgc
     1621 cgtcgatctc ggcgatcgcg cgcgcctggg cgccggtcgg ggcataaggc agcttgagcg
     1681 cgccgcggag acggccgtcg cccctgagcg ggacgccgcg ccgcttgcgc gaagaggcgc
     1741 gcaccagcct cagcgcgagc tggttggcga agatctcgtc ataagcgagc cgctcgcgcg
     1801 ccttcgagtt gttgcgggcg cgatgggcgg tctggagcgc gtcggaccag ccgggccatt
     1861 gccgccggtc gagcaggctc ggctcgatcc actcgccaag ctcgggcacc cgcgccagcg
     1921 cctggccggc gagctggccc aggcggttgt tggtgagccc ttccgagagg ggatagaccg
     1981 gctcgcgctc cggcagggtt gcggcttcct cgggcgggag cacatagtcc ggatggacga
     2041 tctgcagctc ctggccgtac atttccattc ggccggagac gacgcgcggg acgccgagct
     2101 ggagctgctt cttggcccag ccgggattgt tgaaataagt gagcgtcagg taattgccgt
     2161 cgcggtcggc ggcgagcacc cggaacgggc tgcgcgggcc ggcctggcgg taatcgaccg
     2221 gggtgaccgt caccgtcacc acgctgccgg catcggccat gtcgaggatg tcgacgcgct
     2281 tgcgctcgat ccagctcacc ggcaggtgga agaggatgtc gacgacgcgc tcgaggccga
     2341 gccgcttcaa cggcttggcc agcgccgggc cgataccctt cagcacctcg acttcggcga
     2401 acagagggtt gaggatgtcg ggccgcatcg tccgaccgct taagcggagg acgggccgct
     2461 cacaatcctt cgccgggcgc ggtcaggcgc cgtaggcgtg gatcaggtcc acccgcgacc
     2521 agatgatcgc gtaatcggtg gggagatatt cgcccttgtc atagagttcg cgccggctgg
     2581 cggaggtggc cgtcgcgttg gcgatcgccc cttcgttgct gccgaccagc atgcggaagc
     2641 cgccgcggcc gatctgcagg tcctgcgcga agctgagccg cggcgtcacc cgtttgactt
     2701 ccggatcgta gaaccaggcc gtgccgaaga cgccgcgcgc ctccggcaaa gccggcaagg
     2761 cgagcgcggc gagccgcagc gcctcgcccc agccggcctc gttccaatag gccttggcga
     2821 cgggatcgtg cgtatggcct tcgaggaacg gccggcggcc gccgcagcgc aggaagacat
     2881 gcgccgccgc ggccgatccg gcgcgcagca ccgtcttggc cggaagcccc gaatgcggcc
     2941 accagacctg cgcgaaagcg gggatcatca cgacgcgcgc gagccagaga tccttgagga
     3001 agctgtcttt ggcgagatcg gcgaaagcag gatcggcctc gatctggtcg aggatgcggt
     3061 ggaagccatc ggcgtaatgg agcgcgaatt cctcatcgag gccggtggcc gcgaacactt
     3121 cttcgaagcg gccgatatgc cagagcatca gcgcgcacag ccagggggcc ctgaggtccg
     3181 actggatcgc ggccatcatc tccgccgctt cgacgggcac ttcattatat ttgcggatca
     3241 ccgcgtccga ggcccaggcg tcctcgaacc gatcaaaggg caaggcggcg agcgccgcgg
     3301 caaggccggg atggcgctcg gcgagggcgg cggtcgccgg cttgatccga tcggcatagg
     3361 gctcgcgccc gcgccaggtg gggaacaggg cttcggccgg gaccggccgc gcggcgggcg
     3421 agggtcgcat caacatgcgg ccgatatagc agaggatgct tacccggctg ttaccttgag
     3481 gctggtttca gccgggcagc agcgcgcagc ttggcggtat aatgaagcag gccggacaga
     3541 taggcgtcga actcggcgtc gctgacgcgg cggccgtcga gcaatcggcg cagcgccagc
     3601 ggatcggcgc cgggagcgtt cagcccttcg gtgacgaggg tcgccgaatg cacgccggcg
     3661 gcggcgagca aggcgcgggc gcgaggatgg ttgctgccgc tggggtggca gaaatgggtg
     3721 aagggccggt cgccgatggc ggcgcggagg aaggcgcgat tctcctcgac ctcggcggcg
     3781 agcgtgtcga tgcgggtggc gatgtcgatg tgacgatggg tgtggagctg gacgtcgagc
     3841 ccgcgccgct tggcctcggc gaattggtcg gccgacatga tgttgaattg ctggagatcg
     3901 agccaggcct cgtcgacgcc gagctccgcc ccgaaccgac gcagcgccgc cagccggtca
     3961 tcgaggggaa gcgcctcgat cgcggcggtc ttcgcgaccg ggtcgatatc cggacggccg
     4021 ccggcgaggc gcagataatc gaccgcgaca ttgacgacgg ggagcgcccg gccggcatac
     4081 caggtggtgg catagagcgt cgccggcagc gcgtaagctt cgagcaccgg cagcatgtgg
     4141 gtgaaggtgg agacccagcc gtcgtcgatg gtgatcacca ccgccgcctc ggggagggta
     4201 ccgcgggcga gccgatcgac cgcctcggcg agcggcagca ccggccgtcc cgagcgcttg
     4261 agccgcgcca tccgcgcctc gaactgttcg ggcgaaatga aggtgcagtc gccgaattcg
     4321 tagccggggg tcacccacag gccatgatag caaaggatgc gcagctgccc cgccgtcgcc
     4381 ttgcgcgccg ccgccgccag ccccgccgac gatgccgccg cgagaatcct ccgcttcaaa
     4441 gacacgagct cgtctcccac ccccggtaca cgcgctgcca atgcgggtga ggctagcggg
     4501 tgcaggttga aagttcgata atgagcggct gaacgcctcg tgcgggcgct tcaaagccga
     4561 ggggggagcg cctacatggg cgcttgaacc ttccggagcc gagccgccgc cgcgcgtccc
     4621 ggctctgttt cgcatgggag cgagccttgg accccgccga ccgtatcaag cgcctccgct
     4681 atcgcgcctg gcaccgcggc acgcgcgagg cggacttcct gatcggcggc tatttcgacc
     4741 ggcaccatgg cggctggggc gaggcggaga cggcctggtt cgaagccctg atcgaggagc
     4801 aggatgtcga catcatggct tgggcgatcg gaaccgccga gccgcccgct cgctacaacg
     4861 gcggcatgat gcggcgcctc caaaccctcg actatatcaa gcatccggag tgatcgtgga
     4921 cgatatcgaa ctggcgccga agcccgcggc cgaacggagc aggagcggcg gcaaggacaa
     4981 ggcggaggcg cccgcgcgcg tcgacctcca gaccctgttg agggcgaagg agccggtgac
     5041 gctggcgggc gcgccggcgg ggttcgtgcc gtggctggcg gccgacctcg cccgcgccgc
     5101 ggccggcacc ggcggcggcg cccgcgccgt gttcatcgcg tccgacgaag cggcgatgcg
     5161 gcacatcctg gatgccgccc cttatatcgc gccggagctg gagacgctga gcttcccggc
     5221 ctgggactgc ctgccttacg accgcagctc gccttcgctg cgctccacgt cggagcggct
     5281 ggcgacgctg cacgcgctgc agcgcaagcc cgccaagccg cagctcctcg tcaccacggt
     5341 caacgcggcc acccagctga cgctgagccc gttccgcatt cgccagctag tcgcgcggct
     5401 cgcgcccggc gaacggatcg acctcgaccg gctggccgcg ttgctcagcg ccaacggcta
     5461 tatgcgcacc gacactgtcc aggatgcggg cgagttcgcg gtgcgcggcg ggatcgtgga
     5521 cctgttcccc gcgggcgagg agcaggggct gaggctcgac ttcttcggcg acgagatcga
     5581 gagcgtgcgg cgcttcgacc cctcgacgca gcggaccacc ggcaccgccg agggcggctt
     5641 cacgctgctg cccgcctccg aaaccctgct cgacgaaggc agcgtcaagc gcttccggtc
     5701 gcgctaccgc gaattgttcg gcgccaccgc gaccggcgat ccggtctacc aggccgtgtc
     5761 ggagggccgc cgcctggccg gcgtcgacca ttggctgccc ttgttcgagg aacggctcgc
     5821 caccctgttc gaccatctcg gcgaacgcga catcatcgtc cgcgatgccg gccatgccgg
     5881 cgccgccgaa gcccggttcg aggcggtggc ggattattac gagaaccgcg tccgcgccca
     5941 gtccggcgac gccggcagct accggccgct gaagcccgag acgctctatc tgcagccggg
     6001 cgactgggag gcgctgatcg ccgagcggcc gctgcacatc gtctcgccct atcgtgagcc
     6061 gccggggccg gcggtggccg atttcggggt agacggcccg cgcgacttcg cccccgagcg
     6121 cgcgcagaac gtcaatgtct acgaagccgt ggtcgagcat gtcacggcgc tgcgccgcgc
     6181 cggcaagaaa gtggtgctgg cgagctattc gcgcggcgcg cgcgaacggc tgaagggcct
     6241 gctcgaagat catgggctga agaagctggc gctggccgac gggtggcagg aggcgctggg
     6301 cggaccggtc gcgctcaccg tcatccagct cgaccacggc ttcaccaccg ccgacatcgc
     6361 cttgctgacc gagcaggaca tgctcggcga ccggctggtc cgccgccgca agcgcaagaa
     6421 gagccaggat gcgttcctga acgaactcgc cgcgctcagc gccggcgatc tcgtcgtcca
     6481 tgccgatcat ggcatcggcc gctacgaggg gctgacccag atcccggtcg gcagctcgcc
     6541 gcacgattgc gtcgcactcg aatatgccgg cggcgacaag ctctacgtgc cggtcgagaa
     6601 tatcgacgtg ctctcgcgct acggcagcga gagcgacggg gtcgcgctcg accggctcgg
     6661 cggcgaggcg tggcagcggc gcaagtcgcg gatgaaggag cggatccgcg agattgccgg
     6721 cgagctcatc aagaccgcgg ccgcgcgcgc gctgcgcccg ggccaggtcg ccgagccgga
     6781 caccggctac gccgccttcg ccgaccgctt cccctatgag gagacggacg accaggagcg
     6841 ggcgatcacc gacgtcatcg ccgacctcgg cgccgggcgg ccgatggacc ggctggtctg
     6901 cggcgacgtc ggcttcggca agaccgaggt cgcgctccgg gccgcattca tcgccgccat
     6961 gtccgggctc caggtggctt tggtctgccc gactactttg ctcgcgcgcc agcatttcaa
     7021 caccttccgc gagcgcttcc agggcttccc gctcgagatc gggcggctct cccggctggt
     7081 gcccgccgcc gaagccaagg cgaccaagga agggatcaag gacggcaaga tcgacatcgt
     7141 catcggcacc cacgcgatcc tcgccaagag cgtcgagttc aagcagctcg gcctcgtcat
     7201 cgtcgacgag gagcagcgct tcggcgtcac ccacaaggag cggctgaagg cgatgaaggc
     7261 ggacgtccat gtcctcaccc tcaccgcgac gccgatcccg cggacgctgc aaatggcgat
     7321 gtcgggcctg cgcgagctca gcgtcatcca gaccccgccg gtcgaccggc tggcagtgcg
     7381 cacctatgtg atgccgtggg acccggtggt gctgcgcgaa gctttgctgc gcgaacatta
     7441 tcgcggcggc caatcctatt tcgtcgcgcc gcgcatcgcc gatttgcccg acctcgagga
     7501 gtttttgcgc agcgaagtgc ccgaagtgag cttcatcgtc gcccacggcc aaatgtcgcc
     7561 gaccgaggtc gaggagcgga tgtcggcttt ctacgaccgc caatatgacg tgctgctttc
     7621 gaccacgatc gtcgaatccg ggctcgacat accatcggcc aacacgctca tcatccaccg
     7681 cgccgaccgg ttcggccttg cccagctcta ccagctacgt ggccgggtcg ggcgggccaa
     7741 gacccgcgcc tatgcctatt tcacgacgcc gccgacccgc accgtcaccg aaggggcgga
     7801 caagcgcctg caggtgctcg ccaacctcga tacgctcggc gcgggcttcc agcttgccac
     7861 ccacgatctc gacattcgcg gcgccggcaa tctgcttggc gacgagcaat ccggccatat
     7921 caaggaagtc gggttcgagc tttaccagtc gatgctggag gaggcgatcc tggaggccag
     7981 ggccgcgccc ggcgcgcccg tccgcgccgg cgacttctcg cctcagatca cggtcgacgc
     8041 gccgatcatg ctgccggagg aatatgtgcc ggacctagac cttcgcatgg gcctctaccg
     8101 gcgcatgaac gagctggagg gcgggcagga gatcgaatcc ttcgccgccg agctgatcga
     8161 ccgcttcggc aagctgccgc aggcgaccga caatctgctg aaggtgatcg aggtcaaact
     8221 gaactgccgc aaggcgcagg tggtaaagct cgacgtcggt ccgaaggggg cgctggtcca
     8281 cttccacaac gacaccttcc cggacctcga agggctgatc gcctatgtgc agcgcttgaa
     8341 agggaccgca aagctgcggc cggacagcaa gctggtgatc agccgcagct ggccggaccc
     8401 gcagtcgcgg gtcaacggcg cgcttcagct gtcgcgggga ctcgccaaga tcatcggctg
     8461 acggcgcgcc gctccaccgc ctcgagctgc agccaggcgg ccatgcgctc aagctcggca
     8521 tcgagccgtt cggcggtttc ggcgggcgcg ccgggctcga acgtcaccgc ctggaccatg
     8581 agccgcgagc gctggcggtc ggccttgagg tcgacccggg cgacgagcgc ttcgtccatc
     8641 aggaagggca gcacataata gccgtgcacg cgccggtcgg ctggggtgta gatttcgatc
     8701 cggtagcgaa agccgaacaa gcgctcggtg cgcgcccgct cccatacgag gggatcgaac
     8761 gggacgagca gggcctggcc ctcaatccgg cgcgggcggc gtgcctcgcg gtggaggaag
     8821 gcgggcttgc cccagccatc gaccgcgacc ggcagcagca tccccgcctc ggccagctcg
     8881 gcgatggcgg cgcgggcggg ctcgggcgcc agccggaaat agtcgcgcag gtcggtggcg
     8941 gtggcgacgc cgagcgcgcg ggcggcacga tcgatcagcc gccggtgggc ctcggactcg
     9001 tcgggcgtcg gcaaggcgag gatggcgggc gggatgacgc gcttggtgag gtcgtagacc
     9061 cgctcgaagc tgcggcggcg ggtggcggtg gtgacgtggc cggccatgaa gagccattcg
     9121 agcgcgcgct tggccgcgcc ccattcccac cagccgctcc ggctccttgc gccgtcgaag
     9181 tcggaggcgg cgagcggccc ttcggcccgg atccgcgcca gcaccgcttc ggcctcggcg
     9241 cggcgctcgc cggcaaaggc gcgcaggctc ttccagccgc gctcgccgcg gtcggcgcgc
     9301 gccatccgcc agcgcagcaa ggggtggagc gccacgggca gcaaggaggc ttcgtgggcc
     9361 caatattcga acagccggcg ctcgcccagc gggccccagg cggcgcggtc gatgaggccg
     9421 cgatcatagc cgccgaggcg cgagaaagcc ggcagataat gggcgcgggc gagcacgttg
     9481 acgctgtcga tctggtgaag cgagagccgg tcggcgatgc ggcgcaaatg gccgcggtcg
     9541 acctcggccg ggcgcgggag cccgaagccc tgcgcggcca gcgcgacgcg gcgcgcctgg
     9601 ggcagcgaca ggcgttcgat cgggggttgc acgcgcggcg tcagggcggg gaggcgtgga
     9661 cgggcgccgg ctcgggcggc ggcgggctgg cctcgcgcct gcgccggtgg cgcgaccaga
     9721 aggtgtagca ggccggcagc aggatggtat tcacgacgtc gccaatgccc tcggtcagtt
     9781 cgagggtctt gcgccaatcg accgcattct tcaggtcctg cccctcgttg agcaaggccg
     9841 cgaacaggac gacgacgagc ggcagcagat cggccggcgg gcggcggctg agcttgagcg
     9901 cgagcgcata gacgatgacg ccgccgtaaa tgtggagcgc ctgctcgctg aggccgagcg
     9961 cgcccagcag tgccttgaag tcgtgccaga gcatcggggg gtccctaagg cggcgccgtc
    10021 cgggtcacaa tagtggtggc gaaggtggtg cctgaatcga tgatgcgcgg tggagttaag
    10081 ccattgatac tgttatagtt ttatgacttc tgcttcgcgg ccgacggatt cttcccagac
    10141 cgcttcgcgg tgcatttcga agctgccgtc gagcgtgccg aggctccaga cgccggtgac
    10201 gctggtgccg tctccggaca ggcggccgac ataatcgacg gcgtgggcgg cgtcgctggc
    10261 gccgtcatag gctttggtga aatcgaccgc gtggccgtgg cggctgccgc tgaggatcgc
    10321 ttcgagggtg tcgctggtgg cgccgatcgt gttgggctcg atgatggtgc cgccgaggct
    10381 gccgtcatga tcctcgatgc gggcgaggaa gggcgtggtc ggcccctgcc ccgccggata
    10441 ggcgaagctg ccaagccaga ggccgctgag gttgaggttc ggcttggtca cgcgtgcctc
    10501 aatccgcggg cgaggcgagg gttgcagccg gccctacccc gcgaccagcc gggcgagggc
    10561 ggcggtgtcg attgggggcg aacagaggaa gccctggtag agggtgcagc cttcctgggc
    10621 gaggaggccg agctgctcct cggtctcgac gccttcggcg atgacgtcga ggccgagcga
    10681 gcgggccatg tcgatgacgc tgcgcacgac gatgcggtcg cgcggcgagc cggtaatgtc
    10741 ctcgctgagc cgcttgtcga tcttgagata atcgagcgga agcgccttca gataagcgag
    10801 gctcgaatag ccggtgccga aatcgtcgat cgcgatgcgg aggccgccct cgcgcagccg
    10861 ggcgagcagg gacgcggcgg cgttgagatc ctcgatcagg ccgctttcgg tgacttcgac
    10921 ggtgaggcgg cgcggatcga agccgctatc ttcgaccagc gccaggaatt gcgcggcgaa
    10981 gccggggcgg acgatgtcgt cggcggtgat gttgaccgcg aggcggaggg cgcccagcga
    11041 cgccggccag gccgaggcgg cggcgatcgc cttgcgctgg acgtgatcgg acagctggac
    11101 gagatagtcg gagcgttcgg cgacgttgaa cagggtgacc gcgccgagct cgccgaaatc
    11161 ggggtggcgc cagcgcgcca gcgcctcggc gccgacaatg ctgccgttgg tgatcgacac
    11221 ttgcggctgg aacaatatct cgatctcgtc ctggtcgagc gcgcggcgga ggtcgatctc
    11281 gagccggtcg ccgcgcgcgc tgtcgctctg ggcgccggcg tcgaacacgc ggatcggccc
    11341 ggcctcgccg cccttggcgt cgaccaaagc ctcgctggcg cggcgcagca gggcggcggc
    11401 atcgtcgccc gggccggagg cggcgacgcc gacccggctg ccgagcgtga tgacatggtc
    11461 gcccgacatg aacgggcgcc cgatcccctc gacgatctga ccggcgagga agcggccttc
    11521 ggcgaggctg gcgggcggcg cgagcaggac cgcgaattcg gcgccggcga ggcgggcgac
    11581 gaagcggcgg cggccgtcgg cgtcgaccag ccgctcgatg cggcgggcag cggcctgcag
    11641 cacggcgtcg ccggtggcgc ggccgaacgc ggcgttgatc gtgtcgaagc ggctgaccgc
    11701 gagcagcagc agcacgaggt gcggctccgg gcccgcggcc ccgagcaatt gctcctcgat
    11761 ccaggcgcgg gcggcgcggg cgtcgcgcac gccggtcatc ggatcgcgcg actgggtcgc
    11821 gccgctgtca ccggcgccga tcg
//
LOCUS       DASVRK010000272        11821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_245605, whole genome shotgun sequence.
ACCESSION   DASVRK010000272 DASVRK010000000
VERSION     DASVRK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245605"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VF589_10965"
     CDS             complement(<1..968)
                     /locus_tag="VF589_10965"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEX8258143.1"
                     /translation="MVSSVFDLFKIGVGPSSSHTMGPMTAACAFVEMLAEKALLDAVA
                     RVEVDLYGSLALTGKGHATDRAILLGLSGERPHTLCPDHADTVVERIRATGRLMLGGA
                     RDIPFDEPRDLRFLQRERLPHHSNGMRFSALGTDGAPLDAKVYYSTGGGAIVDEAAIA
                     RNAPPEGAWDIPYNYSSADEMLVIAEREGLTIADIARANERAGLSDEEINRRLQAILD
                     AMASCIDRGIAIDGILPGGLKVKRRAPALYRLLMERAERTLQDPLTVLDWVNLWALAV
                     NEENAAGGRVVTAPTNGAAGIVPAILRYYQRFTPRASDEGVQTFLLT"
     gene            1032..2810
                     /gene="pabB"
                     /locus_tag="VF589_10970"
     CDS             1032..2810
                     /gene="pabB"
                     /locus_tag="VF589_10970"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX8258144.1"
                     /translation="MATALIAGEAPFLLFDDARGQGAGPARLYERPVATVTTREPGEV
                     AACLGRLRQARADGLHAAGFLAYEAGLALEAKLAPLARAPAADAPPLLWFGLFESCSE
                     VAASEVPALLPDPASAFAAPPRPLIARPAYEAAADRVKRHIEAGDVYQVNLTFAAEVA
                     TAGHPLALYAALRRRAAAGWGGIAYTGEHWLLSFSPELFFTLAGRRVTTRPMKGTAAR
                     RSDPAEDRKAAAALRQDPKQLAENLMIVDLLRNDLSRVSKPGTVKAPDLFAVETYPTV
                     HQMTSTVTAELEEGVDAIGVLEAMFPCGSVTGAPKIRAMQLIADIETSPRGAYTGSIG
                     WLAADGDAAFNVAIRTLTMRAGEGRAMMGLGSGIVADSRVGEEWRECLAKGAFVAGEG
                     RFDLVETMRFDPHEGVVDLERHLGRMKRSADELGFAFDRHETRNELQAATFRVRQESK
                     LRLLLSRSGAVAVEIRPLPETPPEPVEVAVVRLPVAPDDFRLRHKTSDRSFYDEAREA
                     AGRFEILFEGPDGCLTEGSFTSLFVERGGRLVTPPLARGLMPGVLRGRLLASGEAVEG
                     DIVHADLLGGFLIGNSLRGLIRAVVA"
     gene            2871..3359
                     /gene="msrB"
                     /locus_tag="VF589_10975"
     CDS             2871..3359
                     /gene="msrB"
                     /locus_tag="VF589_10975"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8258145.1"
                     /translation="MKIGRRTLLGAGGAGLLGGTLLFRGGPSEAREGNFEVKLSDAEW
                     KKRLTPQQYAVLRRESTERAGSSPLNKEKRRGIYSCAGCGLPVYSSATKFESGTGWPS
                     FWKPLANAVGTREDNSLLFKRTEVHCRRCGGHLGHVFDDGPKPTGLRYCMNGVAMKFR
                     AA"
     gene            complement(3390..4052)
                     /gene="pdeM"
                     /locus_tag="VF589_10980"
     CDS             complement(3390..4052)
                     /gene="pdeM"
                     /locus_tag="VF589_10980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEX8258146.1"
                     /translation="MVPFSFAGHDLTALPQGALFWPARRALLVADLHLEKASWFARSG
                     QMLPPYDSMATLGDLAALAVTTGAAEIWCLGDSFHDSHGCERLPERAREMLTALTAAT
                     RWTWITGNHDPVYADPCGGRVVAEAEVDGLLLRHEADPAEPRPELSGHFHPKLRISQR
                     GRQVSRRCFVATATKLILPAFGALTGGLDAGHPEIVRAVGPDAQALVPLADRLLKFPI
                     AA"
     gene            complement(4098..4253)
                     /locus_tag="VF589_10985"
     CDS             complement(4098..4253)
                     /locus_tag="VF589_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258147.1"
                     /translation="MKIKQTLQNPFVLVAQGFVVGTLIFLSTAPRDDNGASAADRVAS
                     AVEKIGA"
     gene            complement(4435..4932)
                     /locus_tag="VF589_10990"
     CDS             complement(4435..4932)
                     /locus_tag="VF589_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX8258148.1"
                     /translation="MRSFDFTPYRRSTVGFDRLFDFLESAARSEQVDNYPPYDIEKVS
                     DDSYRITLAVAGFRADEIDITARQNMMVITGRKAENRNKDGNYLHIGIATRAFERRFE
                     LADFVRVENAELRDGLLSIELVREIPDAMKPRKIAINGAAPQTIEANSRERSETADQR
                     ETEAA"
     gene            complement(5056..7137)
                     /locus_tag="VF589_10995"
     CDS             complement(5056..7137)
                     /locus_tag="VF589_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8258149.1"
                     /translation="MPAPPPGSSAGGPGTPQGGEAERLRQLFDKAPGFMAATRGPEHV
                     FEMANASYLQLVGRSDLIGKTVRESFPDLEGQGFFERLDKVYRTGQAFSGRSVPLSLR
                     RQPDAGSEERLLDFVFQPIKDERGEVTGLFVEGYDVTEWHEAERALRESEERFRLIAD
                     SAPVPMWVTKLDRRRGFVNIAYAEFLGVAYDEALDFDWRTIIHPDDIGWLLAESLAGE
                     ATLKPFALEGRYRRADGEWRWLRSESQPRWGPDGEHVGFIGVAHDITDAKHAEAALRE
                     VNETLERRVAERTADLTAALDRLQAEVAERQRAEEALRQAQKMEAVGQLTGGIAHDFN
                     NLLTPIMGGLEFIATRTDDARLKRIAETALDSARRGAKLTGQLLAFSRIQRISMAPIA
                     VNQVIANMKDLLRHTIGKEVEVQTRLDPAVGHALCDANQLENALLNLAINARDAMPEG
                     GVVTIFTSALRVESEPDLDDGDYVRVSVVDTGEGMPDEILARATEPFFSTKPLGKGTG
                     LGLAQVYGIAHQSGGTIRIDSEVGKGTAVHLLLPAVAAAGEAPPTPPSRAIEAAAPAL
                     PAAGATILVIDDDEDVRAFLATALENLGYDVIDAEGGEAGLAKLAESGADLMLVDYAM
                     PGMHGADVARAARASHPKLPIVFVTGYAETSQLEAALGQDVPVLRKPFSGAELAAIVA
                     ANLAPAAAPTS"
     gene            complement(7142..8341)
                     /locus_tag="VF589_11000"
     CDS             complement(7142..8341)
                     /locus_tag="VF589_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX8258150.1"
                     /translation="MRLSLLLSLSLIVPLAASAHETVSPADRLSHEQMLVLDTHLDTP
                     VHFERPGWDFDRWHEYQWDLTQTDIPRMEAGGLDGGFFVIYTPQGALTPQGMTQARDA
                     ALGRARAIQRVAAANSDKLGLAYTAADAERLHREGKRIVYQSIENSYPLGNDLSLLAA
                     FHKMGVRMAGPVHSKNNQFADSTTDTPRWNGLSPLGRQWVAEMNRLGMVVDASHASDA
                     AFDQMLKLSKTPIILSHSGPRAIFNHPRNLDDARMRKLARAGGVMQMNSVFLVQHDNS
                     DERSAVEKRQERWLDLTPAERRRLVADKAALDARQPYTAADFEMFMRSMLHAIKVMGV
                     DHVGLGADWDGGGGVRGMEDVAALPRITARLRKEGFSDRDIEKMWSGNTLRLLRQAER
                     QAQRRSS"
     gene            8475..9377
                     /locus_tag="VF589_11005"
     CDS             8475..9377
                     /locus_tag="VF589_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8258151.1"
                     /translation="MTDAARAIQEQAASSPADIRASGWVEVLKRSWAESSKDNVNIIA
                     AGVAFYAFLAFVPLLAAMMLTYGLVAEPSSVVRHMQALTGMMPADAAQLIGQQLMSMS
                     QTAQGQKGFGLILALLLAIYGAMRGSSSIITALNVVYNVEEDRGFIRSTLLALALTLG
                     ALVALLVAILAVSSLGYIEALLPSAAPWLHALLRILFWVAAAAVISTLVAMIYRYAPN
                     RPNPKWRWLTPGSLAATFLWVVATLGFGFYVANFGNYNATYGSLGAVIVFLTWLYLTA
                     YILLMGGELNAELEKQTSCNTLAP"
     gene            complement(9379..9834)
                     /locus_tag="VF589_11010"
     CDS             complement(9379..9834)
                     /locus_tag="VF589_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258152.1"
                     /translation="MRYFLDTEFNGFGGELISLALVPEHGDQEYYALLPVPDPVHPWV
                     ERHVVPYLRSVPPGLDGGTVTRLQAAAEIADYLAPDDNPLILADWPDDIAYFCALLVT
                     GEGEMAPVGTLHFELIRSPGFSTAAISAVPHNALHDARALREFVLRWQA"
     gene            complement(9892..10659)
                     /locus_tag="VF589_11015"
     CDS             complement(9892..10659)
                     /locus_tag="VF589_11015"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX8258153.1"
                     /translation="MGANIRLRGAALALLLGACMPAADKSAAAPAANHARGELGAKPQ
                     AGDHSGAAGEHEIIVAGAEPALLYVPASYRREAPMPLLLMLHGAGGTARHSVDLASRY
                     ADRFGFIILAPASRASTWDVIAGRAYGSDVAAIDAALAQSFAAYAVDRSRVAIGGFSD
                     GASYALSLGLANGELFGRILAFSPGFVAPGRQAGRPQIFISHGVADRVLPIEATSRRI
                     VPRLRDAGYKVDYEEFRGGHIVPAELASRAFESWVKD"
     gene            complement(10660..11061)
                     /locus_tag="VF589_11020"
     CDS             complement(10660..11061)
                     /locus_tag="VF589_11020"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX8258154.1"
                     /translation="MIDRLPSPPGGPHLRTIAMPRDANPSGDIFGGWTLSQMDLAGAT
                     FAAERAGGRVATIAVDAMKFLHPIAVGDEVTCFCSAEEETAVSVKVRVETWTRSPGGE
                     DAEKVTHGLFTFVAVTEEGKPRRLPEGGEGK"
     gene            11221..>11821
                     /locus_tag="VF589_11025"
     CDS             11221..>11821
                     /locus_tag="VF589_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258155.1"
                     /translation="MLAVSTIALTMLALQSAAAPPALPAPRTADAATLDDEFDGAALS
                     SDWTVFHSAYGWPDKIKAIDVGRTTEGALHFEPYHSGWVRDLNAPFLFKTVEGDFDVR
                     ARVRVKGLNGEVPGGTWSLGGLMARVPNGLKAADWKPRAENWHFITTGVGLEPGKVMT
                     ETKGTYNSYSSLKLRPYRSGWVELRMVRSGMALFVLARPD"
BASE COUNT         1775 a         3915 c         4266 g         1865 t
ORIGIN      
        1 gtgaggagga aggtctgcac gccctcgtcc gacgcgcggg gagtgaagcg ctgatagtaa
       61 cgaaggatgg cggggacgat gccggcggcg ccgttggtcg gcgcggtgac gacgcggccg
      121 ccggcggcat tctcctcgtt gaccgccagc gcccacagat tgacccagtc gagcacggtg
      181 agcggatcct gcagcgtgcg ctcggcccgc tccatcagca gccggtagag ggcgggcgcg
      241 cggcgcttca ccttgagccc gccgggcagg atgccgtcga tggcgatgcc gcggtcgatg
      301 cagctcgcca tggcgtcgag gatcgcctgc agccggcggt tgatctcctc gtcggagagg
      361 ccggcgcgct cgttggcgcg ggcgatgtcg gcgatggtca gcccttcgcg ctcggcgatc
      421 accagcatct cgtcggccga gctataatta tagggtatgt cccaggcgcc ctcgggcggc
      481 gcgttgcggg cgatcgcggc ttcgtcgacg atcgcgccgc cgccggtcga ataatagacc
      541 ttggcgtcga gcggcgcgcc gtcggtgccg agcgcgctga agcgcatgcc gttggaatgg
      601 tgcggcagcc gctcgcgctg caggaagcga aggtcgcgcg gctcgtcgaa cgggatgtcg
      661 cgcgcgccgc ccagcatgag gcggccggtg gcgcggatgc gctcgacgac cgtgtcggca
      721 tgatcggggc agagcgtatg cgggcgctcg cccgacaggc cgagcaggat ggcacggtcg
      781 gtggcgtggc ccttgccggt cagcgcgagc gaaccgtaga ggtcgacctc gacccgcgcg
      841 acggcgtcca gcagcgcttt ttcggccagc atctcgacaa aggcgcaggc ggccgtcatc
      901 ggacccatgg tgtgcgaact tgaagggccg accccgatct tgaacagatc gaagacactg
      961 gaaaccatgc accttctcct caaggctcgg gcccacccgg ccttcgggcc agatgggctt
     1021 ccggcgccgc aatggcaacc gcgctgatcg cgggcgaggc gcccttcctg ctgttcgacg
     1081 atgcgcgcgg acaaggcgcc gggccggccc gcctctacga gcggccggtc gccaccgtca
     1141 cgacgcgcga gccgggggag gtggcggcat gcctcgggcg gctgcggcag gcgcgggccg
     1201 acgggctcca cgccgccggc ttcctggcct atgaagcggg gctggcgctg gaggcgaagc
     1261 tggcgccgct ggcgcgggct ccggccgcgg acgcgccgcc cttattgtgg ttcggcctgt
     1321 tcgaaagttg cagcgaagtt gcggcatccg aggtgccggc gctgctgccg gatcccgcct
     1381 ccgctttcgc cgcgccgccg cgaccgctga ttgcccgccc ggcctacgag gcggcggcgg
     1441 accgggtgaa gcgccacatc gaggccggcg acgtctacca ggtcaacctc accttcgccg
     1501 ccgaagtggc gacggccggc catccgctcg cgctctatgc ggcgcttcgc cgccgggcgg
     1561 cggcgggctg gggcggcatc gcctataccg gcgagcattg gctgctctcc ttttcgcccg
     1621 aattgttctt caccctcgcc ggccggcggg tgacgacgcg gccgatgaag gggacggcgg
     1681 cgcggcggag cgacccggcc gaggaccgca aggcggcggc ggcgcttcgc caggacccca
     1741 agcagctcgc cgaaaatctg atgatcgtcg atctcctgcg caacgacctg tcgcgcgtgt
     1801 cgaagcccgg cacggtcaag gcgcccgatt tgttcgcggt cgagacctat ccgaccgtcc
     1861 atcagatgac ctccaccgtg acggccgaac tcgaggaggg agtcgatgcg atcggcgttc
     1921 tcgaggcgat gttcccgtgc ggctcggtga ccggcgcgcc caagatacgg gcgatgcaat
     1981 tgatcgccga catcgaaacg tcgccgcgcg gcgcctatac cgggtcgatc ggctggctgg
     2041 cggcggatgg cgatgccgct ttcaatgtcg cgatcaggac cttaaccatg cgcgccggcg
     2101 aggggcgcgc gatgatgggg ctcggctccg gcatcgtcgc cgacagccgc gtgggcgagg
     2161 agtggcgcga gtgcctcgcg aagggagcgt ttgtggcggg ggaagggcgg ttcgacctgg
     2221 tggagacgat gcgcttcgac ccgcatgagg gggtcgtcga cctcgagcgc cacctcggcc
     2281 ggatgaagcg cagcgccgac gaactcggct tcgccttcga ccgccacgag actcgcaacg
     2341 agcttcaggc ggcgaccttc cgggtccgcc aggaaagcaa gctccggctg ctgctgtcgc
     2401 gcagcggcgc ggtcgcggtc gagatccggc cgctgccgga aacgccgccg gagccggtcg
     2461 aggtcgcggt ggtgcggctg ccggtcgcgc ccgacgactt ccggctccgc cacaagacca
     2521 gcgatcgcag cttctacgac gaggcgcgcg aagcggccgg ccgcttcgag atcctgttcg
     2581 aagggccgga cggctgcctc accgaaggca gcttcaccag cctgttcgtc gagcgcggcg
     2641 gccggctggt gacgccgccg ctggcgcgcg ggctgatgcc gggcgtgctt cggggccggc
     2701 tgctcgccag cggcgaggcg gtggagggcg atatcgtcca tgccgatctg ctcggcggct
     2761 tcctgatcgg caattcgctt cgcgggctga tccgcgcggt ggtcgcgtga acggcgttca
     2821 ccggtggctc accggcgggc ggctaggccg gcgcaaacgg aggtagaggc atgaagatcg
     2881 gtcgcaggac cttgctgggc gcgggcggcg cggggctgct gggcgggact ttgctgtttc
     2941 gcggcggacc gtccgaggcg cgcgagggca atttcgaggt caagctcagc gacgccgaat
     3001 ggaagaaacg gctgacgccg cagcaatatg cggtgctgcg ccgggagagc accgagcgcg
     3061 cgggctcgag cccgctcaac aaggagaagc gccgcggaat ctattcctgc gccggctgcg
     3121 gcctgccggt ttattcctcg gcgaccaagt tcgagagcgg caccggctgg ccgagcttct
     3181 ggaagccgct cgccaatgcg gtcggcacgc gggaggataa cagcctgctg ttcaagcgca
     3241 ccgaggtcca ttgccggcgc tgcggcggcc atctcggcca tgtcttcgac gacggcccca
     3301 agccgaccgg gcttcgctat tgcatgaacg gcgtggccat gaagttccgc gccgcctgat
     3361 ccgtcgggcc tccgcgcgac cgctggatat caggcggcga tcgggaattt gaggaggcgg
     3421 tcggcgagcg ggaccagcgc ctgggcgtcg gggccgacgg cgcggacgat ctcgggatgg
     3481 ccggcatcga ggccgccggt gagcgcgccg aaggcgggca ggatgagctt ggtcgcggtg
     3541 gcgacgaagc agcgccgcga gacctgacgg ccgcgctggc tgatcctgag cttgggatgg
     3601 aagtggccgg aaagctcggg ccgcggctcg gccgggtcgg cctcgtggcg gagcaggagg
     3661 ccgtcgacct cggcttcggc gacgacgcgg ccgccgcaag ggtcggcata gaccgggtcg
     3721 tgattgccgg tgatccaggt ccagcgggtg gcggcggtga gcgcggtcag catttcgcgc
     3781 gcgcgctcgg gcaggcgctc gcagccgtgg ctgtcgtgga acgagtcgcc gaggcaccag
     3841 atttccgcgg ccccggtcgt gacggcgagc gcggccagat cgccgagggt ggccatcgaa
     3901 tcgtagggcg gcagcatctg ccccgagcgg gcgaaccagc tcgctttttc gaggtggagg
     3961 tcggcgacca gcaaggcgcg gcgcgcgggc cagaacaagg ccccctgcgg gagggcggtc
     4021 agatcgtggc cggcgaacga aaagggaacc atctcccccg ctttaaacgg gcgggcggcg
     4081 cttgcaagcg cgtccaatca ggcgccgatc ttttcgacgg cggaagcgac gcggtcggcg
     4141 gcggaggcgc cattgtcgtc gcgcggggcg gtcgacagga agatcaaggt cccgacgaca
     4201 aatccctgcg ccaccagcac gaacgggttc tgcaatgtct gcttgatctt catggctctg
     4261 ctctcggttc gaaaaatttg ctgtcggact gtccggtctt ctgtgcacgg ccgtctctaa
     4321 acgaggcctg aatcgctcac gagcctcgct cgacgaaagt gcggagcgcc gcgacgaaaa
     4381 tgaaaaagat ccggcgccgg ggcagcgccc ggcgccggag cttcagggtc cggatcaggc
     4441 ggcttcggtt tcgcgctggt cggcggtctc gctgcgctcg cggctgttcg cctcgatcgt
     4501 ctggggggcg gcgccgttga tcgcgatctt gcgcggcttc atcgcgtcgg ggatctcgcg
     4561 caccagctcg atcgagagca ggccgtcgcg aagctcggca ttctcgaccc ggacgaagtc
     4621 ggccagctcg aagcgccgct cgaacgcgcg ggtggcgatg ccgatgtgga ggtaattgcc
     4681 gtccttgttg cggttctccg ccttgcggcc ggtgatcacc atcatgttct gccgcgcggt
     4741 gatgtcgatc tcgtcggcgc ggaagccggc cacggcgagc gtgatgcgat agctgtcatc
     4801 gctcaccttc tcgatgtcgt aaggcgggta attgtcgacc tgctcggacc gggcggcgct
     4861 ttcgaggaag tcgaacagcc ggtcgaagcc gacggtggaa cggcgatagg gagtaaaatc
     4921 gaagcttctc atcatgtcct ccttgctgaa gcaacatcaa tgaggggcgg ttcggagcga
     4981 agcgcccctg atacggaccg gaaactgcaa acaagtcttc cggctcacgg aatctaggaa
     5041 acgcccattt gggcttcaag aggtcggggc ggccgcgggc gcgaggttgg ccgccacgat
     5101 cgccgccagt tcggcgccgc tgaacggctt gcgcagcacc ggcacgtcct ggccgagcgc
     5161 cgcctcgagc tggctggtct cggcatagcc ggtgacgaag acgatcggca gcttagggtg
     5221 gctggcgcgg gcggcgcggg cgacgtcggc gccgtgcatg cccggcatgg cgtaatcgac
     5281 cagcatcagg tcggcgcccg actcggcgag cttcgcgagg cccgcttcgc cgccctcggc
     5341 gtcaatcacg tcatagccga gattttcgag cgcggtggcg aggaaggccc gcacatcctc
     5401 gtcgtcgtcg atcaccagga tggtggcgcc cgcggcgggc aaagcggggg cggcggcctc
     5461 gatcgcgcgg ctgggcggcg tcggcggggc ctcgcccgcc gcggcgaccg ccggcagcag
     5521 gagatggacg gccgtgccct tgccgacttc gctgtcgatg cggatcgtgc cgccggactg
     5581 gtgggcgatg ccgtaaacct gggcgaggcc gaggccggtg cccttgccga gcggcttggt
     5641 cgagaaaaag ggttcggtgg cgcgggcgag gatttcatcc ggcatgccct cgccggtgtc
     5701 gaccacagag acgcggacat agtcgccgtc gtcgaggtcg ggctcggatt cgacccggag
     5761 cgcgctggtg aagatcgtca cgacgccgcc ttcgggcatc gcgtcgcggg cgttgatggc
     5821 gaggttcagg agcgcatttt cgagctggtt ggcgtcgcac aaagcgtggc cgacggcggg
     5881 gtcgagccgc gtctggacct cgacctcctt gccgatcgtg tggcggagca ggtccttcat
     5941 gttggcgatc acctggttga ccgcgatcgg cgccatgctg atccgctgga tgcgcgagaa
     6001 agcgagcaac tggccggtga gcttggcgcc gcgccgggcc gaatcgagcg cggtctcggc
     6061 gatccgtttc agccgggcat cgtcggtccg ggttgcgatg aattcgaggc cgcccatgat
     6121 cggcgtcagc agattgttga aatcgtgggc gataccgccg gtgagctggc cgaccgcctc
     6181 catcttctgg gcctggcgca gcgcctcttc ggcgcgctgg cgctcggcga cctcggcctg
     6241 caggcggtcg agcgcggcgg tgaggtcggc ggtgcgctcc gccacccttc gctccagcgt
     6301 ctcgttgacc tcgcgcagcg ccgcttcggc atgtttggcg tcggtgatgt cgtgggcgac
     6361 gccgatgaag ccgacatgct cgccgtccgg cccccagcgc ggctgcgatt cggagcgcag
     6421 ccagcgccac tcgccgtcgg cgcgccggta gcggccttcg agcgcgaacg gcttcagcgt
     6481 cgcctcgccg gccagggact cggcgagcag ccagccgata tcgtcggggt ggatgatggt
     6541 gcgccagtcg aagtcgagcg cttcgtcata ggcaacgccg aggaactcgg cataagcgat
     6601 gttgacgaag ccgcggcggc ggtcgagctt ggtcacccac atcggcaccg gcgccgaatc
     6661 ggcgatcagc cggaaccgct cctcgctctc gcgcagcgcg cgctcggctt cgtgccattc
     6721 ggtgacgtca tagccctcga cgaacagccc ggtgacctcg ccgcgttcgt ccttgatcgg
     6781 ctggaacacg aaatcgagca gccgttcctc ggagcctgcg tcgggctgcc gccgcagcga
     6841 cagcgggacc gagcgcccgg aaaaggcctg gccggtccgg tacaccttgt cgagccgctc
     6901 gaaaaagccc tgcccctcca gatcggggaa gctctcgcgc acggtcttgc cgatgaggtc
     6961 gctacgcccg accagctgga gataggaggc gttggccatt tcgaacacgt gctcggggcc
     7021 acgtgtggcg gccatgaagc ccggcgcctt gtcgaacagc tggcgcagcc gctccgcctc
     7081 gccgccctgc ggggtccctg gtccgccggc ggacgaccca ggcggaggcg ctggcatgcc
     7141 gtcaggagct ccgccgctgc gcctgccgct cggcctggcg cagcagccgc agcgtgttgc
     7201 cgctccacat cttctcgatg tcgcggtcgc tgaagccttc cttgcggagc cgcgccgtga
     7261 tccgcggcag agcggcgaca tcctccatgc cgcgcacgcc gccgccgccg tcccagtcgg
     7321 cgccgagccc gacatggtcg acccccatca ccttgatcgc gtgcagcatg ctccgcatga
     7381 acatctcgaa atcggccgcg gtgtaaggct ggcgggcgtc gagcgccgcc ttgtcggcga
     7441 ccagccggcg ccgctcggcg ggagtgagat ccagccaccg ttcctggcgc ttctcgaccg
     7501 cgctgcgctc gtccgaattg tcgtgctgga cgaggaacac cgagttcatc tgcatgacgc
     7561 cgcccgcgcg ggccagcttg cgcatgcgcg cgtcgtcgag gttgcgcgga tggttgaaga
     7621 tcgcgcgcgg gccggaatgg gagaggatga tcggcgtctt cgacagcttc agcatctggt
     7681 cgaaggcggc gtcggaggcg tggctggcgt cgaccaccat gccgagccgg ttcatctccg
     7741 ccacccattg ccgcccgagc gggctcagcc cgttccagcg cggcgtgtcg gtggtcgaat
     7801 cggcgaactg gttgttcttc gaatggaccg gcccggccat gcgcacgccc atcttgtgga
     7861 aggcggccag cagcgacagg tcgttgccga gcgggtagct gttctcgatg ctctggtaga
     7921 cgatccgctt gccctcgcgg tggaggcgct cggcgtcggc ggcggtgtag gccagcccga
     7981 gcttgtcgct attggcggcg gccacgcgct ggatcgcccg cgcccgtccc agtgccgcgt
     8041 cgcgggcctg ggtcatgccc tgtggcgtca acgcgccctg cggcgtgtag atgacgaaga
     8101 agccgccatc gagcccgccc gcctccatgc gcgggatgtc ggtctgggtg aggtcccatt
     8161 gatattcgtg ccagcggtcg aagtcccagc cgggccgctc gaaatggacc ggcgtgtcga
     8221 gatgggtgtc gagcaccagc atctgctcgt ggctgagccg gtcggccggg ctcaccgtct
     8281 catgggcgga agccgcgagc ggcacgatca gtgacagcga aagcaacagg ctgaggcgca
     8341 ttccaaactc ccggtgatgg cggcgccagc atgtaagcat gatcggcatt aggcaagcgc
     8401 atgacgggtc gccgaaaaag gcgggaaccc ttgccgatgc tccggccttt gcattcgcgc
     8461 gagggagacg agcgatgacc gatgcggcac gggcgatcca ggaacaagcg gcgtccagcc
     8521 cggcggatat ccgcgcgtcg ggctgggtcg aggtgctgaa gcgcagctgg gccgaatcga
     8581 gcaaggacaa tgtcaacatc atcgcggcgg gcgtcgcttt ctacgccttc ttggccttcg
     8641 tgccgttgct ggcggcgatg atgctgacct atgggctggt cgccgagccg tcctcggtgg
     8701 tgcgccacat gcaggcgctg accgggatga tgccggccga cgcggcccag ctgatcggcc
     8761 agcaattgat gagcatgagc cagacggccc aggggcagaa gggcttcggg ctgatcctcg
     8821 ccttgctgct cgccatctac ggcgcgatgc gcggctcgtc ctcgatcatc accgccttga
     8881 acgtggtcta caatgtcgag gaggatcgcg gcttcatccg cagcaccctg ctcgcgctgg
     8941 cgctgacctt gggggcgctg gtcgccttgc tggtcgcgat cctggcggta tcgtcgctcg
     9001 gctatatcga ggccctgctg ccgagcgcgg cgccgtggct gcacgctttg ctcaggatcc
     9061 tgttctgggt cgcggccgcg gcggtgatca gcaccctcgt cgcgatgatc taccgctacg
     9121 cgcccaaccg gccgaacccg aaatggcgct ggctgacgcc cggctcgctc gccgccacct
     9181 tcctgtgggt ggtggcgacg ctcggcttcg gcttctacgt cgccaatttc ggcaattaca
     9241 acgccaccta cggctcgctc ggcgcggtga tcgtgttcct gacttggctc tatctcaccg
     9301 cctacatcct actgatgggc ggcgagctca acgccgagct cgaaaagcag accagctgca
     9361 acacgctcgc cccctgaact aggcctgcca gcgcagcacg aattcgcgca gcgcgcgggc
     9421 gtcgtggagg gcgttgtggg gcacggccga gattgccgcg gtgctgaagc cggggctgcg
     9481 gatcagctcg aaatggagcg tcccgaccgg cgccatctcg ccctcgccgg tcaccagcaa
     9541 agcgcagaaa taggcaatgt cgtcgggcca gtcggccagg atcagcggat tatcgtcggg
     9601 cgcgagatag tcggcgatct cggccgccgc ctgcagccgc gtcaccgtgc cgccgtcgag
     9661 tccgggcggc accgagcgca gataggggac gacatggcgc tcgacccacg ggtgaaccgg
     9721 gtccggcacc ggcagcaagg cataatattc ctggtcgcca tgctcgggca ccaaagcgag
     9781 gctgatgagc tcgccgccga agccattgaa ctcggtgtcg aggaaatagc gcatggggcg
     9841 gcgctattcc cgttcccggc gccgctgtcc acccgccggg gagcgcttcg gtcagtcctt
     9901 cacccagctt tcgaatgcgc gcgacgccag ctccgccggc acgatatggc cgccgcgaaa
     9961 ctcctcgtaa tcgactttat agccggcgtc gcggagccgc ggcacgatgc ggcggctggt
    10021 cgcctcgatc ggcagcaccc ggtcggcgac gccgtggctg atgaagattt gcggccgccc
    10081 cgcctgccgg cccggagcga cgaagccggg tgagaaagcg aggatgcggc cgaacaattc
    10141 gccattggcg aggccgagcg acagagcata ggaggcgccg tcggaaaagc cgccgatcgc
    10201 cacccgcgac cggtcgaccg cataagccgc gaagctttgc gcgagcgccg cgtcgatggc
    10261 cgcgacgtcg gagccgtagg cgcggccggc gatgacgtcc caagtcgacg cgcgcgaggc
    10321 cggggcgagg atgatgaagc cgaaacggtc ggcatagcgg ctggcgaggt cgaccgaatg
    10381 gcgcgcggtc ccgccggcac cgtggagcat cagcagcagc ggcatcggcg cttcgcggcg
    10441 atagcttgcc ggcacgtaga gcagggccgg ctccgctccg gcgacaatga tctcgtgctc
    10501 gcccgccgcg ccggaatgat cgccggcctg gggcttggcg ccgagctcgc cacgtgcatg
    10561 gttggcggcc ggcgccgccg cggacttgtc agcggccggc atgcaggccc ccagcagcag
    10621 ggcgagggcg gcgccgcgga gccgaatatt cgcgcccatt cacttgccct ccccgccttc
    10681 cggcaggcgg cgcggcttgc cctcctcggt cacggcgacg aaggtgaaca ggccgtgggt
    10741 gaccttctcg gcatcctcgc cgccgggcga gcgggtccag gtctcgaccc ggaccttgac
    10801 cgagaccgcc gtctcctcct cggccgagca gaagcaggtc acttcgtcgc cgaccgcgat
    10861 cgggtgcagg aacttcatcg cgtcgaccgc gatcgtcgcc acccgcccgc cggcgcgctc
    10921 ggcggcgaag gtcgctccgg ccaggtccat ctgcgacaag gtccagccgc cgaaaatgtc
    10981 gccggacggg ttggcgtcgc gcggcatggc gatggtgcgc agatgcggcc cgcccggcgg
    11041 ggatggaagt cggtcgatca taacacgggt tccggcatag gtcggggctg aacgcgcggg
    11101 cgccgtgccg gtgcccgccg ctaagccttc gtcgaagccg cgccgccgct ggtcgaaaac
    11161 cggtccccgc cgccgcgccg gtggtcagtt tcccgcaaaa tcctcgcgaa aggcccccaa
    11221 atgctcgctg tttcgacgat tgccctgaca atgctcgcgc tccagtccgc cgcagcgccg
    11281 ccggcgctgc cggcaccgcg cacggccgat gccgccacgc tcgacgacga gttcgacggc
    11341 gccgcgctgt cctccgactg gaccgtcttc cattcggctt atggctggcc cgacaagatc
    11401 aaggcgatcg acgtcggccg caccaccgag ggcgcgctcc acttcgaacc ttatcattcc
    11461 ggctgggtgc gcgacctcaa cgcgccgttc ctgttcaaga cggtcgaagg cgatttcgac
    11521 gtccgcgcgc gcgtgcgggt gaagggcctg aacggcgagg tgccgggcgg cacctggtcg
    11581 ctgggcggcc tgatggcgcg ggtgccgaac ggcctcaagg cggccgactg gaagccgcgg
    11641 gcggagaatt ggcatttcat caccaccggc gtggggctcg agcccggcaa ggtgatgacc
    11701 gagaccaagg gcacctataa cagctattcc tcgctgaaac tccgccccta tcgcagcggc
    11761 tgggtcgagc ttcgcatggt gcgctcgggc atggccctgt tcgtgctggc gcggccggac
    11821 g
//
LOCUS       DASVRK010000273         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_430878, whole genome shotgun sequence.
ACCESSION   DASVRK010000273 DASVRK010000000
VERSION     DASVRK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430878"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..862)
                     /locus_tag="VF589_11030"
     CDS             complement(62..862)
                     /locus_tag="VF589_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8258156.1"
                     /translation="MTIRTILVDDEPLAIQGLQLRLEKHDDVDIVETCSNGREAIRAI
                     KTHKPDLVFLDIQMPGFDGFSVIQGLMEVEPPLFVFVTAYSDHAIKAFEAQAVDYLMK
                     PVEEVRLADTLDRVRQRLAEKRGVEEVGRLKEVLAEVAPDAVDTSDYAEDAHQSNRYE
                     KLINIKDRGQIFRVDVDTIEKIDAAGDYMCIYTGDNTLILRETMKDLEKRLDPRRFQR
                     VHRSCIVNLDLVRQVKPHTNGECFLVLDSGSQVKVSRSYRDVVARFVH"
     gene            complement(859..1959)
                     /locus_tag="VF589_11035"
     CDS             complement(859..1959)
                     /locus_tag="VF589_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8258157.1"
                     /translation="MRNPFGLQQQPFFEDKNRAFWMLQSAGWAGYFVLRTLSGIANAM
                     GWTYVLHTALLTATGYSITLLMAAAYRRLIKQKPIVTWAFSIALVLTASASFSAIETW
                     SYATFLSPGFRPEGVQFFSSILLTFSLLAAWSALYYGINYYLLLEEQSDKLLRLQTQA
                     SSAQLAMLRYQLNPHFLFNTLNSISTLVLLKQTDRANAMLSRLSSFLRYTLVNEPTGQ
                     VTVEQEVETLKLYLEIEKMRFEERLRPRFHIDPAAAGARLPSLLLQPLVENAIKYAVT
                     PQEEGADIAIDARKVGDRVVITVSDTGPGTDSQYTVRAQQSTGVGLANIRDRLAQAYG
                     ADHRFETQTNIRGGFGVIIEIPYQYEPAEEAK"
     gene            complement(2094..>3588)
                     /locus_tag="VF589_11040"
     CDS             complement(2094..>3588)
                     /locus_tag="VF589_11040"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8258158.1"
                     /translation="AAATRALLAGEGPRLVLAGPEPVPAPAVEAALAAAQKAPPAARQ
                     ADRKVSMDALPPIGGAGREVARRRIEDMGVTIVEFANGSTLTFKQTDFEKGSVAVQLR
                     FGAGLAGQRPDQPSLAWLSGAVVPSGVAGLDLDGLERLLTGRKMGINFGVDEDAFVLS
                     GRTNGTDLGDELRLLASKLAFPRWDGSVVDRIKTSAVESYDLAFASAAARAGREFSGF
                     ARPNDARWAPVEKAQIAAATPAQFEAFFAPLMGAGPVQAIIVGDVDLETTVAAMKKSI
                     TALPARPPVRVPEAHRAVAPPRPNPSPATFTHVGDKDQAFALIGWTTFGGTDRIRERR
                     ALVMAAHLLQVRLFERLREAEGSSYSPSAVSTTSQDYPDWGIFYAASELRPESADDFF
                     RIAREIVADLAAKPAAEDEYARARNPILSGIERRVKTNGFWLESMEDWITQPELIAQT
                     RNYYADYETLTAEDVRAAVAKHVRDAGDWSMLVVPGRPAAAAAAAPR"
BASE COUNT          583 a         1230 c         1172 g          603 t
ORIGIN      
        1 ggttggcttt ggaagccggc cttcgcgggg cagccaagaa gaagccgcgc cgcccgaaag
       61 cctaatggac gaaccgagct accacgtccc ggtaggagcg gctgaccttc acctggctcc
      121 cggaatcgag caccaggaaa cattcgccgt tggtgtgcgg cttgacctgc cggaccaggt
      181 ccaaattgac gatgcagctc cggtgcacgc gctggaagcg gcgcgggtcg aggcgcttct
      241 cgaggtcctt catggtctcg cgcaggatca gcgtgttgtc gccggtatag atgcacatat
      301 agtcgcccgc ggcgtcgatc ttctcgatcg tgtcgacgtc gacgcggaag atctggccgc
      361 gatccttgat gttgatcagc ttctcatagc ggttcgactg atgcgcgtcc tcggcatagt
      421 cgctggtgtc gaccgcgtcg ggggcgacct cggcgagcac ttccttcaag cggccgacct
      481 cctcgacgcc gcgcttctcg gcgagccgct ggcgcacccg gtcgagcgtg tcggcgagcc
      541 gcacctcctc gaccggcttc atcagataat ccaccgcctg cgcttcgaac gccttgatcg
      601 catggtcgga ataagcggtc acgaacacga acaagggcgg ctccacctcc atcaggccct
      661 ggatcaccga aaagccgtcg aagccgggca tctggatgtc gaggaaaacg aggtccggct
      721 tgtgcgtctt gatcgcgcgg atcgcttccc ggccgttcga gcaggtctcg acgatgtcga
      781 cgtcgtcatg cttctccagg cgcaattgca ggccctggat ggcaagcggc tcgtcatcga
      841 ccaggatggt tcgaatggtc atttggcttc ctcggctggt tcatactggt aggggatttc
      901 gatgataacg ccaaacccgc cccttatgtt cgtttgggtc tcaaaacgat ggtcggcgcc
      961 ataggcttgc gccagccggt cgcgaatgtt ggcgaggccg accccggtcg attgctgcgc
     1021 gcgcaccgta tattggctgt cggtgcccgg cccggtgtct gaaacggtga tcaccacccg
     1081 atcgccgacc ttgcgggcgt cgatcgcgat gtccgcccct tcctcctgcg gcgtcaccgc
     1141 atatttgatc gcattctcga ccaaaggctg gagcagcagc gacggcagcc gcgcccccgc
     1201 cgcggccgga tcgatatgga agcgcggccg cagccgctcc tcgaagcgca tcttctcgat
     1261 ctcgagatag agcttcagcg tctccacctc ctgctccacc gtcacctgcc cggtcggctc
     1321 gttgaccaag gtgtagcgca ggaacgagga tagccgcgag agcatcgcat tggcccggtc
     1381 ggtctgcttc agcaggacca aagtcgagat cgaattcagc gtgttgaaca ggaaatgcgg
     1441 gttgagctgg tagcgcagca tcgccagctg ggccgaactc gcctgggtct ggagccgcag
     1501 cagcttgtcg ctctgctcct cgagcagcaa ataataattg atcccgtaat agagcgccga
     1561 ccaggccgcg agcagcgaga aggtcagcag gatcgagctg aagaattgca ccccctcggg
     1621 ccggaagccg gggctgagga aggtcgcata gctccacgtc tcgatcgccg agaagctcgc
     1681 cgaggcggtc agcaccaacg cgatcgagaa ggcccaggtg acgatcggct tctgcttgat
     1741 cagccgccgg taggccgccg ccatcagcaa ggtgatcgaa taaccggtcg cggtgaggag
     1801 ggcggtgtga aggacgtagg tccagcccat cgcattggcg atgccgctca aggtgcgcag
     1861 cacgaaatag ccggcccagc cggccgattg cagcatccag aaggcgcggt tcttgtcctc
     1921 gaagaagggc tgctgctgga gcccgaacgg gttccgcatc ttcttcgcct cgcgctgcgc
     1981 gggcgtcgtc aagctgatcg gttcagtggc caccaggttc ggctctaagg aatctccgct
     2041 cgaatgaaaa ccgccccgtc attccaccag ccccgccgcc tgccgccgca ccctcagcgc
     2101 ggagccgccg ccgctgccgc ggccgggcgc cccggcacca ccagcatcga ccagtcgccg
     2161 gcgtcgcgga catgcttcgc aaccgccgcc cgcacgtcct cggccgtcag cgtctcgtaa
     2221 tcggcgtaat aattccgcgt ctgcgcgatc agctcgggct gggtgatcca gtcctccatc
     2281 gattccagcc agaagccgtt ggtcttcacc cgccgctcga tgccgctgag gatcgggttc
     2341 ctggcgcgcg catactcgtc ctccgccgcc ggcttggcgg cgaggtcggc gacgatctcg
     2401 cgggcgatcc ggaaaaagtc gtcggcgctc tccggccgca attccgaggc ggcgtagaaa
     2461 atgccccaat cgggataatc ctgcgacgtg gtcgacaccg cgctcggcga atagctcgac
     2521 ccctccgcct cgcgcagccg ctcgaacagc cgcacctgga gcaggtgcgc ggccatcacc
     2581 agggcgcgcc gctcgcggat ccggtcggtg ccgccgaaag tggtccagcc gatcagcgcg
     2641 aacgcctggt ccttgtcgcc gacatgggtg aaggtggcgg gcgacgggtt gggcctgggc
     2701 ggcgccaccg cgcggtgcgc ttccggcacc cgcaccggcg gccgggcggg aagggcggtg
     2761 atgctcttct tcatcgccgc gaccgtggtt tcgaggtcga cgtcgccgac gatgatcgcc
     2821 tgcaccggcc ccgctcccat cagcggggcg aagaaggctt cgaactgggc cggcgtcgcg
     2881 gccgcgatct gggccttctc gaccggcgcc cagcgcgcat cgttgggccg cgcgaagccg
     2941 gaaaattccc gccccgcccg cgccgccgcc gaagcgaagg cgaggtcgta gctttcgacc
     3001 gcgctggtct tgatccggtc gaccaccgag ccgtcccagc gcggaaaggc gagcttgctg
     3061 gccagcagcc gcagctcgtc gccgagatcg gtgccgttgg tccggccgct cagcacgaag
     3121 gcatcctcgt cgaccccgaa attgatcccc atcttgcggc cggtcagcag ccgctcgagc
     3181 ccgtccaggt cgagcccggc gacgccggac gggaccaccg ctccgctcag ccaggccagc
     3241 gagggctggt ccggccgctg ccccgccagc ccggcgccga agcgcagctg gacggcgacc
     3301 gagcctttct cgaagtcggt ctgcttgaag gtcagcgtcg atccattggc gaattcgacg
     3361 atggtgacgc ccatatcctc gatccgtcgc cgggcgactt cgcggcctgc gccgccgatc
     3421 ggcggcagcg cgtccatgct gaccttgcgg tcggcctggc gcgcggccgg cggcgctttc
     3481 tgcgcggcgg cgagggcggc ctcgaccgcg ggcgcgggca ccggctccgg ccccgccagc
     3541 accagccgcg gcccttcgcc cgccagcagc gcgcgcgtgg ccgccgcg
//
LOCUS       DASVRK010000274        16553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_431083, whole genome shotgun sequence.
ACCESSION   DASVRK010000274 DASVRK010000000
VERSION     DASVRK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16553
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431083"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VF589_11045"
     CDS             complement(<1..353)
                     /locus_tag="VF589_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8258159.1"
                     /translation="MSTSPSDARPILVTGGAGFIGCNIADALAGAGHEVLVYDALSRP
                     GVETNLAWLKDRHGGRIDSIVADIRDEDSLARAAREAKAVFHMAAQVAVTTSMGDPRE
                     DFEVNLKGTINLLDAL"
     gene            complement(505..1620)
                     /locus_tag="VF589_11050"
     CDS             complement(505..1620)
                     /locus_tag="VF589_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8258160.1"
                     /translation="MTERILITGGAGFIGRAVARELLGRGHQVRVLDSLIEQVHGDRE
                     RPEDLPHEVELIRADVRNGDAVAKSLQGVDSVIHLAAEVGVGQSMYAVERYTSVNDVG
                     TAVLFEKLIDHPVRRVVTASSMSIYGEGLYRDADRNLVQEARRAPRTAEDQSWDPLDV
                     QGRPLKPVATPETKRPDLASIYALNKYVQERTTMIMAEAYGMEGACLRLFNVYGPGQA
                     LSNPYTGVLAIFASRLLNGQQPMIFEDGEQRRDFVYVGDVARAFAEALELPQAVGEIF
                     NVGSGNDRSVTEVAQELARAMGKNDISPQIVGKSRIGDIRHCFCDTSKTEERLGFRAT
                     KDFQEGLAELAEWVAVQTADDRVDEARAELEARGLVA"
     gene            1848..2447
                     /locus_tag="VF589_11055"
     CDS             1848..2447
                     /locus_tag="VF589_11055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8258161.1"
                     /translation="MTATILLIRHAAHVELGRVLSGRRRDVALSKEGLEQAEIVADLL
                     GVEPLAAVYSSPRERAYYTARQVAEQHELKVSVADGLDEVDFGDWSGASFDALEGDPL
                     WQQWNEARGSARPPGGESMAEAAARAAAQVQELAADHSDQTIAAVSHCDIIRGLIAHY
                     LGLPLDNLLRFDIDPASVSRLMVGAWGARVISVNERLYQ"
     gene            2444..3247
                     /locus_tag="VF589_11060"
     CDS             2444..3247
                     /locus_tag="VF589_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HEX8258162.1"
                     /translation="MTLAARTITAADAALRKRIEALGPWFHNIQLAGIWTAPDHFLGD
                     YPNEKFQRFAPHLPEDLSGKTVLDIGCNAGFYSMEMKRRGASRVLGIDSDDRYLAQAR
                     LAGEALGYDNIEFRNLSVYDVGALGEKFDVVIFMGVLYHLRHPLLALDLIHEHVAGDL
                     LIFQSMQRGSRQVLELEEDYDFEETGMFLESGFPKLHFIERQYAHDWTNWWVPNRACA
                     EAMLRASGFSIEIRAEEEVYICRRSEMPYADWGNAGAVYPAAGAATGRE"
     gene            3248..4198
                     /locus_tag="VF589_11065"
     CDS             3248..4198
                     /locus_tag="VF589_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEX8258163.1"
                     /translation="MIEAAMIWNEPNNKSHWDPELDPDWSIFSRTVVEAGKAIAAVNP
                     AVKRVLGGMSPIDPHFIERLKGNGVLDHVDVIAVHGFPLDWNLWPIHEWPDRIAEIEA
                     VAPDHEIWATEVGVGSFGAEEVQVFGVDRTAELLLGRVPRVYWYSLFDLPQSWEATTR
                     HKQAEGSSYYRHFYMGLIKEDGTPKLALDHFARHTPEMGIMQWFHYEDHRLDDAVAWM
                     RRLGVKRLRTGLSWWDSYRPGWERWFDRQMDALAEFDTTVTFCFTPDCEGIQPHHTSP
                     PRDPMFFADFCARMIERYAPAQRQTPVPSPVQAPVQAKVA"
     gene            4198..5304
                     /locus_tag="VF589_11070"
     CDS             4198..5304
                     /locus_tag="VF589_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX8258164.1"
                     /translation="MALRDPHAINVAIVGVGNCASSLVQGVAHYQNGGANEQIGLMHW
                     DLGGYRPKDISFVAAWDVDRRKVGRDLGEAIFAKPNCTAIFCDHVPASGATVKMGRVL
                     DGISDHMADMGDDRTFLLADAAEPDKADVVRTLRETQADVLLNYLPVGSQQATEFYAE
                     CALEAGVAFVNNIPVFIASDPAWARRFAEAGVPIIGDDIKAQLGATIVHRVLTDLFRK
                     RGVKLDRTYQLNTGGNTDFLNMSNRSRLKSKKISKTEAVQSVAEHRLDDENIHIGPSD
                     YVAWQNDNKVCFLRMEGQLFGGVPMNLELRLSVEDSPNSAGVAIDMIRCAKLAKDRGL
                     GGPIHPAAAYFCKHPPEQITDDAAYEALEAFIRG"
     gene            complement(5311..5805)
                     /locus_tag="VF589_11075"
     CDS             complement(5311..5805)
                     /locus_tag="VF589_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX8258165.1"
                     /translation="MTALAADADQTETFRQAMRRVASTVNVISICVDGVPMGITATAM
                     SSLAMDPPSLLVCINRAASLYGSIRDVSHFGVNVLHRDQEHLAQMFADRSKHALRFAD
                     GWELDCERPPRLIDAQAALLCRRTDHHQFGTHSIFIGVVEDVVVRPEVDPLVYLDGKY
                     GSAG"
     gene            5952..6584
                     /locus_tag="VF589_11080"
     CDS             5952..6584
                     /locus_tag="VF589_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HEX8258166.1"
                     /translation="MIEFITAPENLPFAVALILMAMIGLVEAIGLGAGAAHLDFHAGA
                     DGDLLGWLGIGRVPLLILLVVFLALFGMIGLAVQQLAAALAGVPMAPLNASAVALVAA
                     LPLLGVSARALARIMPSDETTAIGLDSLIAKRATVTIGTARRGSPARAQVRDVHGQTH
                     YVMIEPQGDDQSIAAGETVLLVRREGDIFIGLIEGDAYLPRLDDRPALIR"
     gene            6597..8270
                     /locus_tag="VF589_11085"
     CDS             6597..8270
                     /locus_tag="VF589_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HEX8258167.1"
                     /translation="MPFMNLAIYAGVVLATLITLGLIITRLYRRATKEIGFVRTGFGG
                     EKVVINGGALVLPVLHETMPVNLNTVRLAVERKNNDALITLDRLRIDVKAEFYVRVRP
                     DRESIAIAAQTLGLRTMQPEHLKELIEGKFVDALRSVAAGMTMNQLHEQRSEFVQKVQ
                     QVSSADLAMNGLELESVSLTGLDQTSIDHFNANNAFDAEGLTKLTEQIELRRKARNDI
                     EQETRVQIEAKNLESARKSFEISRDTEFARLEQEREVEIRRAAQNAEVAQQQSERQRE
                     ADEARITAKRQVDSAQIEANRVVEQAKITQAQSLEIARQEQQIAVQNKSREESQAKAE
                     ADTARAAAVAAEEKVGTARETEIAERVKRIELIDASREAERAAIGIKVQAEAAKQAAA
                     DHAEATRLGAEGEAEAEKLRAEASRVRFEVEAAGQRAINEAANLLSSDQISLQTKMAL
                     LKVLPEVVREAAKPMEAIDSIKIIQVDGLTGAGAGSGPGGAGVPSGDGNLASSAVAAA
                     LRYRAQAPIVDGLMKELGFDGSTLENLVAGAAAKPADEPAVTVVTTPRK"
     gene            complement(8354..10456)
                     /locus_tag="VF589_11090"
     CDS             complement(8354..10456)
                     /locus_tag="VF589_11090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8258168.1"
                     /translation="MRTAILGIASILALTVAAATPAQQPPAAQPAPGKPAWGTFGVEF
                     DSMDRAVHPGDDFWSYVNGRWARTVEIPADRGALSQVARLNDLSMSQVRAILDEYNGR
                     RGSLAGDDRRIADYYASLMDQAAIEKRGAAPLLADLKPIRAAASHSALAALMGGLVRE
                     WQGTPPLGRMPRYFPSPFGLGIGQDSKEPNRYVPGIGQGGLGMPSRDYYLKEDGDAAA
                     TQTAYHGHLAAMLALTGVAKAEAAQRAAAVYQFERGIAEAHWPLADARDADKTYNPWT
                     LAELRSRAPGFDWDAFLGGAGLARRPAYIVGETTAISAMAKLFGATPLPVLKDWLAIR
                     FAKDRALVLPAAFAREEFAFSGGTLGGATEAPARWLQAVELTATAMTDAVSRPYIERH
                     FPAETKAAMDDLVKNVLAAMDRRLANLSWMAPETKVKARAKLAAFTPMIGRPDTWRSY
                     EGLRVARGDPYANLKAAARFAWQRDLARIDRPVDRREWWMMPITANAYASFQNNQIVF
                     PAGYLQAPHFDPRADPAVNYGAIGYVIGHEISHHFDDQGSKFDPEGRLVKWWTDQDLA
                     RFKERAQKLVAQYDAYEALPGLNVRGAQTLGENIADNAGLAIAWDAYQLSLGGKPAPV
                     IDGFTGAERFFMGRAQVNKVAFREAELRKAVLSGVHSPSKWRTWSVRNHDAWYDAFGV
                     KPGHKLYLPAEERVKVWE"
     gene            complement(10768..11655)
                     /locus_tag="VF589_11095"
     CDS             complement(10768..11655)
                     /locus_tag="VF589_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX8258169.1"
                     /translation="MNPTSAWPRLSELIVSQGPGDVALLGAPMEAKSVTPGRYDLAPG
                     VIRETLRRFSRYDVETGQELGLRIYDAGDVAVEGRTPEEGFAPIGRAVAGCTAAHPLT
                     LLLGGHNAITRPAAHGLGLPLGRVGLITLDAHFDLRDTDEGLLNGNPIRALLEDRMPG
                     GNICQIGLAPFANTARMHGIARGAGIGVYTIADCKRRGILAVIDEALGRLGHVDAVMV
                     DFDIDVIDRAQCPGAPGARPGGMPADMFFEAARYLGRQRQVKLVDLTEFDPALDVGEV
                     TALTAGRWVCEVLAGFSRR"
     gene            11734..12906
                     /gene="hutI"
                     /locus_tag="VF589_11100"
     CDS             11734..12906
                     /gene="hutI"
                     /locus_tag="VF589_11100"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX8258170.1"
                     /translation="MDPAVPGAFGAIENGAIGIADGRIVRVGRRTELAGYRAREVVPL
                     GGAWVTPGLVDCHTHLVFGGNRSAEYEMRLEGASYEEIARAGGGIGATVKATRAASIE
                     ALVRAARPRLQAMMRGGVTTIEIKSGYGLNTDGELKMLEAVRALGDSEAVRVVPTLLA
                     LHALPADFKDRRADFVRMAVDEMLPAAAARGLAQAVDAFAEGIGFTVDEVRALFAAAR
                     RHGLPVKLHAEQLSNLSGAAMAAEYGALSADHLEHVDEAGVAAMAAAGMVAVLLPGAF
                     YALSETKKPPVDLLRAHGVPMAVATDCNPGTSPVLSPTLMLSMACTLFGLTPEEALAG
                     MTREGARALGLQDSLGTISTGKAADLCVWRIGRPAELSYWIGLPGPERRIVGGVDA"
     gene            13037..14695
                     /gene="hutU"
                     /locus_tag="VF589_11105"
     CDS             13037..14695
                     /gene="hutU"
                     /locus_tag="VF589_11105"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX8258171.1"
                     /translation="MNRRDNSRNIRARTGPEIKARSWSTEAAVRMLMNNLDPDVAEAP
                     EELVVYGGIGRAARDWESFDRIVETLERLGEDETLLVQSGKPVGVFRTHEDAPRVLLA
                     NSNLVPKWATWEHFNALDRKGLMMYGQMTAGSWIYIGTQGIVQGTYETFAEMGRQHYG
                     GDLAGKWILTAGLGGMGGAQPLAAVMAGAHCLAIECQQSRIEKRLATRYLDKATASLD
                     EALQMLSEATSPTSVGLLGNAAEILPEMLKRGIRPHALTDQTSAHDPVNGYLPIGWTV
                     EQWIERRERDPKAVEHAARASMAVHVEAMLAYREMGIPTFDYGNNIRQVARDEGVEHA
                     FDFPGFVPAYVRPLFCRGIGPFRWVALSGDPEDIYRTDAKVKELIPDDAHLHRWLDMA
                     RERIAFQGLPARICWVGLGQRHRLGLAFNEMVARGELKAPIVIGRDHLDSGSVASPNR
                     ETEAMKDGSDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGYSQHAGMVIVADGTAA
                     AARRLERVLWNDPGTGVMRHADAGYDIAIDCAREQDLDLPMVGR"
     gene            14692..16209
                     /gene="hutH"
                     /locus_tag="VF589_11110"
     CDS             14692..16209
                     /gene="hutH"
                     /locus_tag="VF589_11110"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX8258172.1"
                     /translation="MTSPITLRPGSVTLAELRAIWAGAPVALADEAWRAVEAAAESVA
                     RIVASGRTVYGVNTGFGLLAQTRIPDDRLLDLQRNLILSHSCGLGDALDPRVVRLIMV
                     LKVIGLARGHSGVTRALVERLLALIEAEAFPVVPAQGSVGASGDLAPLAHLSAALIGE
                     GAILLGGERMPAADALGKLGLEPLVLGPKEGLALINGTQVSTAVALDALFAGERVFGS
                     AVVAGAMSVDALKGTDVAFDPRIHEARGQPGQIAVAAALKRLLGGSAIRESHAGCPRV
                     QDPYSFRCQPQVMGAALDLMRSVARTLEIEANAVTDNPVIFGDDAVSGGNFHAEPVAF
                     AADMLAMALCEVGSISERRTAALVDPKMSGLPPFLVQDSGVNSGFMIAQVTAAALVSE
                     NKSLAFPASVDSIPTSAGQEDHVSMATHGAVKARRIAGNAAGVIGIELLAAAQGLDFH
                     APLRTSPALVPVHQAIRAEIPHYASDRYLADELAWAKREVLEGRLGEELRAELFK"
     gene            16223..>16553
                     /locus_tag="VF589_11115"
     CDS             16223..>16553
                     /locus_tag="VF589_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8258173.1"
                     /translation="MIDLDQAPAQAELATEVCVIGAGAAGISVTRKLLAAGHEVVLLE
                     SGGLDYEADVAELNTGENVGEDYYDLEDSRLRFFGGTTAIWGGRCAELDPIDLEKRDY
                     VPHSGWPI"
BASE COUNT         2682 a         5815 c         5486 g         2570 t
ORIGIN      
        1 agcgcgtcga gcaggttgat cgtacccttg aggttgacct cgaaatcctc gcgcgggtcg
       61 cccatcgagg tggtgacggc gacctgggcg gccatatgga acaccgcctt ggcctcgcgc
      121 gcggcgcggg cgagcgaatc ctcgtcgcgt atgtcggcga cgatgctgtc gatgcggccg
      181 ccatggcggt ccttgagcca agccaaattg gtctcgaccc ccggccgcga cagcgcgtca
      241 tagaccagca cctcatgccc cgccccggcc agcgcatcgg cgatgttgca accgataaac
      301 ccggccccgc cagtgacgag gatcgggcgt gcgtcggagg gggaggtgct catttcgctc
      361 ctccccggca cggggagggg gaccgcgacg cgaagcggcg tggtggaggg ggccctccgt
      421 cggcactgct cagggtccgg gccccctcca ccgcgcttcg cgcggtcccc ctccccgttc
      481 cggggaggat ttcaaagacc cccctcacgc gaccagcccc cgggcctcga gctcggcgcg
      541 ggcctcgtcg acgcggtcgt cggcggtctg caccgccacc cattcggcaa gctcggccaa
      601 cccttcctgg aaatccttgg ttgccctgaa gccgagcctc tcctcggtct tggacgtgtc
      661 gcagaagcaa tgccggatat cgccgatccg cgacttgccc acgatctgcg gcgagatgtc
      721 gttcttgccc atcgccctgg cgagctcctg cgcgacctcg gtcaccgagc ggtcgttgcc
      781 cgacccgacg ttgaaaatct cgccgaccgc ctgcggcagc tcgagcgctt cggcgaaggc
      841 gcgggcgacg tcgccgacat agacgaagtc gcgcctttgc tcgccatcct cgaagatcat
      901 cggctgctgc ccgttgagca atcgcgaggc gaagatggcg aggacgcccg tataggggtt
      961 ggaaagcgcc tggcccggcc cgtagacgtt gaacaggcgc aggcacgcgc cctccatgcc
     1021 ataggcctcg gccatgatca tggtggtgcg ctcctgcaca tatttgttga gcgcatagat
     1081 ggaggcgagg tcgggccgct tggtctccgg cgtggcgacc ggcttcagcg gccggccctg
     1141 gacgtcgagc ggatcccagc tctgatcctc ggcggtgcgc ggcgcgcgcc gcgcttcctg
     1201 caccagattg cggtcggcgt cgcgatagag cccctcgcca tagatgctca tcgaggaggc
     1261 ggtgacgacg cggcgcaccg gatggtcgat caatttctcg aacagaacgg cggtgccgac
     1321 gtcgttgacc gacgtgtagc gctcgaccgc atacatggac tggccgaccc cgacctcggc
     1381 ggcgaggtgg atgacgctgt cgacgccctg gagcgacttg gcgacggcat cgccgttgcg
     1441 cacgtcggcg cggatcagct cgacctcatg cggcagatcc tcgggccgct cgcggtcgcc
     1501 atggacctgc tcgatgaggc tgtcgaggac ccggacctga tggccgcgtc cgagcagttc
     1561 ccgcgccacc gccctgccga tgaaaccggc gccgccggtg atgagtatgc gctcagtcaa
     1621 tgactacccc cgtggtggtt tgatccagga ttagggaagg tgaattaacc cagattagct
     1681 tcttcatctt cttcttcccg gctcccatcc cgccaacccg agattcccgg gtttgttccg
     1741 taaccgtctc atttggtgtc actctcaacc cgcggtggaa ccgcagtgca accccgtagg
     1801 ttgggcagca aaagccaagg tcgtttcatg tcggtatcga tttcagcgtg acggcaacta
     1861 tcctcctcat ccgtcatgca gcacatgtcg aactgggccg cgttctatcc ggacggagac
     1921 gggatgtggc gttgagcaag gaaggtctgg agcaagccga gatcgtcgcc gacctgctcg
     1981 gcgtggagcc gctcgcggcc gtctattcga gcccgcgtga gcgcgcttat tataccgccc
     2041 gccaggtcgc cgagcagcat gagctcaagg tcagcgtcgc cgacgggctc gacgaggtgg
     2101 atttcggcga ctggagcggc gccagcttcg acgcgctcga gggcgacccc ttgtggcagc
     2161 aatggaacga ggcccgcggc agcgcccgcc cgcccggcgg cgagagcatg gccgaggccg
     2221 cggcccgcgc cgccgcgcag gtccaggagc tggccgccga tcattccgac cagaccatcg
     2281 ccgcggtcag ccattgcgac atcatccgtg ggctgatcgc tcattatctc gggctgccgc
     2341 tcgacaatct gctgcgcttc gacatcgatc ccgcctcggt ctcccggctg atggtggggg
     2401 cgtggggagc gcgcgtcatc agcgtgaatg agaggctcta ccaatgacgc ttgccgccag
     2461 gacgattacg gcggccgacg ccgcgctccg caagcgcatc gaggcgctgg ggccgtggtt
     2521 ccacaatatc cagctcgccg ggatctggac cgcgcccgac catttcttgg gcgattatcc
     2581 caacgagaag ttccagcgtt tcgcgccgca tttgccggag gatctgagcg gcaagaccgt
     2641 gctcgacatc ggctgcaacg ccggcttcta ttcgatggag atgaagcggc gcggcgcgag
     2701 ccgggtgctc ggcatcgatt ccgacgaccg ctacctcgcc caggcgcggc tggcgggcga
     2761 ggcgctcggc tacgacaata tcgagttccg caacctctcc gtctacgatg tcggcgcgct
     2821 cggcgagaaa ttcgacgtcg tcatcttcat gggcgtgctc taccacctcc gccatccttt
     2881 gctggcgctc gacctcatcc acgagcatgt cgccggcgac cttttgatct tccagtcgat
     2941 gcagcgcggc tcgcgccagg tgctggagct ggaggaggat tacgatttcg aggagaccgg
     3001 catgttcctc gaaagcggct ttcccaagct gcacttcatc gagcgccaat atgcccacga
     3061 ctggaccaat tggtgggtgc cgaaccgggc ctgcgccgag gccatgctcc gcgcctccgg
     3121 cttttcgatc gagattcggg ccgaggagga ggtctacatt tgtcggcgca gtgaaatgcc
     3181 ttatgcagat tggggcaatg cgggcgcggt ctatcccgcg gccggcgcgg caacagggag
     3241 agaataattg atcgaagcgg cgatgatctg gaacgagccg aacaacaagt cccattggga
     3301 cccggagctc gatcccgatt ggtcgatctt ttcgcgcacc gtggtcgagg ccggcaaggc
     3361 gatcgccgcg gtcaacccgg ccgtgaaacg ggtgctgggc ggcatgtcgc cgatcgaccc
     3421 gcacttcatc gagcggctga agggcaatgg ggtgctcgac catgttgacg tcatcgccgt
     3481 ccacggcttc ccgctcgact ggaatttgtg gccgatccac gaatggccgg accgcatcgc
     3541 cgagatcgag gcggtcgcgc ccgaccatga gatctgggcg accgaggtcg gcgtcggcag
     3601 cttcggcgcc gaggaggtgc aggtgttcgg ggtcgaccgc accgccgaat tgctgctcgg
     3661 ccgggtgccg cgcgtctatt ggtacagcct gttcgatctg ccgcagagct gggaggcgac
     3721 cacccgccac aagcaggccg aaggatcgag ctattaccgc catttctaca tgggcctgat
     3781 caaggaagac ggcacgccca agctcgcgct cgaccatttc gcgcggcaca cgcccgaaat
     3841 gggcatcatg caatggtttc attacgagga tcatcgcctc gacgacgcgg tcgcctggat
     3901 gcggcggctc ggggtcaaaa ggctgcgcac cgggcttagc tggtgggaca gctaccgccc
     3961 cggctgggaa cgctggttcg accgccagat ggacgcgctg gccgagttcg acaccacggt
     4021 gacgttttgc ttcacgcccg attgcgaggg catccagccg caccatacca gcccgccgcg
     4081 cgacccgatg ttcttcgccg atttctgcgc acggatgatc gagcgctacg cgcccgccca
     4141 gcgccagacg cccgtgccgt cgcccgtcca agcccccgtc caggcaaagg tggcctgatg
     4201 gcgcttcgcg atccgcacgc catcaacgtc gccatcgtcg gcgtcggcaa ttgcgcctcc
     4261 tcgctcgtcc agggcgtggc gcattaccag aatggcgggg ccaacgagca gatcggcctc
     4321 atgcactggg acctgggcgg ctaccggccc aaggacatca gcttcgtcgc cgcctgggac
     4381 gtcgaccgcc gcaaggtcgg ccgcgatctc ggcgaggcga tcttcgccaa acccaattgt
     4441 accgcgatct tctgcgacca cgtgccggcg agcggcgcca ccgtgaagat gggccgcgtg
     4501 ctcgacggca tctccgatca tatggccgat atgggcgacg accgcacctt cctgctcgcc
     4561 gacgcggccg agccggacaa ggccgacgtg gtccgcacgc ttcgcgagac ccaggccgac
     4621 gtgctgctca actatctgcc ggtcggaagc cagcaggcga ccgaattcta tgccgaatgc
     4681 gcgctcgagg cgggggtggc gttcgtcaac aacatcccgg tcttcatcgc cagcgatccg
     4741 gcctgggcgc gccgcttcgc cgaggccggc gttccgatca tcggcgatga catcaaggcg
     4801 caattgggcg cgaccatcgt ccaccgcgtc ctcaccgacc tgttccggaa gcgcggcgtc
     4861 aagctcgacc gcacctacca gctcaacacc ggcggcaata ccgacttcct caacatgtcg
     4921 aaccgctcgc ggctgaaatc gaagaagatc tccaagaccg aggcggtcca gtcggtcgcc
     4981 gagcaccggc tcgacgacga gaatatccat atcgggccca gcgactatgt cgcctggcag
     5041 aatgacaaca aggtctgctt cctgcgcatg gaggggcaat tgttcggcgg cgtgccgatg
     5101 aacctcgaac tgcgcctgtc ggtcgaagat tcgcccaatt cggccggggt cgcgatcgac
     5161 atgatccgct gcgcgaagct cgccaaggac cgcggcctcg gcggcccgat ccaccccgcc
     5221 gccgcctatt tttgcaagca cccccccgaa cagatcaccg acgacgccgc ctacgaagcc
     5281 ctggaagcct tcattcgcgg gtgagggcgt ctagccagcg ctgccgtact tcccatcgag
     5341 atacaccaaa gggtccacct ccggccgcac caccacgtcc tcgaccacgc cgatgaagat
     5401 cgagtgggtg ccgaactggt ggtggtcggt gcggcggcag agcaaggccg cctgcgcgtc
     5461 gatcagccgc ggcggccgct cgcagtccag ctcccagccg tcggcgaagc gcaaagcgtg
     5521 cttggagcgg tcggcgaaca tctgggcgag atgctcctgg tcgcggtgga ggacattgac
     5581 tccgaagtgc gacacgtccc tgatcgaccc gtagagcgag gcggcgcggt tgatgcacac
     5641 caagaggctc ggcgggtcca tcgccagcga cgacatggcg gtggcggtga tccccatcgg
     5701 caccccgtcg acgcagatcg agatgacgtt gaccgtcgag gcgacgcggc gcatcgcctg
     5761 gcggaacgtc tcggtctggt cggcgtcggc ggcaagggcg gtcatcggcg ttagctgggg
     5821 cttgcgaggg caaaaggcaa tcggatgcgg ccggggcagc cgccgccggg agcggcagga
     5881 gcagcagcaa acctccgatt ttgctggata atttcgggcg cttttgataa tcgcggggac
     5941 aggggagcgg aatgatcgag ttcatcacag cgccggaaaa cctgccgttc gcggtggcgt
     6001 tgatcctgat ggccatgatc ggcctcgtcg aggctatcgg cctcggcgcc ggcgccgcgc
     6061 atctcgattt ccacgccggc gccgatggcg acctgctcgg ctggctcggc atcggccgcg
     6121 tcccgctgct gatcctgctg gtcgtcttcc tggccttgtt cggcatgatc ggcctcgccg
     6181 tccagcagct cgccgccgcg ctcgccggag tgccgatggc gcccctgaac gccagcgccg
     6241 tcgcgctcgt cgccgccctg cccttgctcg gcgtcagcgc ccgcgccctc gcccgcatca
     6301 tgcccagcga cgagaccacc gcgatcggcc tcgacagcct gatcgccaag cgcgccaccg
     6361 tcaccatcgg caccgcccgc cgcggctcgc ccgcccgcgc ccaggtccgc gacgtccacg
     6421 gtcagaccca ttatgtcatg atcgagccgc agggcgacga ccagagcatc gccgccggcg
     6481 agaccgtgct gctcgtccgc cgcgaaggcg acatcttcat cggcctgatc gaaggcgacg
     6541 cgtatcttcc gcgcctcgac gaccggccgg ccctcatcag ataaacggag agacacatgc
     6601 ctttcatgaa cctggccatc tatgcgggcg tcgtcctcgc gacgttgatc acgctcggcc
     6661 tcatcatcac ccgcctctac cgccgcgcga cgaaggagat cggcttcgtg cgcaccggct
     6721 tcggcggcga gaaggtggtg atcaacggcg gcgccctcgt cctgccggtc ctccacgaga
     6781 ccatgccggt caatttgaac accgtccgcc tcgccgtcga gcgcaagaat aacgacgcgc
     6841 ttatcacgct cgatcgcctg cgcatcgacg tgaaggccga attctacgtc cgcgtccgcc
     6901 ccgaccgcga atcgatcgcc atcgccgccc agacgctcgg cctccgcacg atgcagcccg
     6961 agcatctgaa ggagctgatc gagggcaagt tcgtcgacgc gcttcgctcg gtcgccgccg
     7021 gcatgaccat gaaccagctc cacgagcagc gctccgaatt cgtccagaag gtgcagcagg
     7081 tctcgtccgc cgatctcgcc atgaacggcc tcgagctcga aagcgtgtcg ctgaccggcc
     7141 tcgaccagac ctcgatcgac catttcaacg ccaacaacgc gtttgatgcc gaggggctca
     7201 cgaagctcac cgagcagatc gagctccgcc gcaaggcgcg caacgacatc gagcaggaaa
     7261 cccgggtcca gatcgaggcc aagaacctcg aatcggcgcg caagagcttc gagatttcgc
     7321 gcgacaccga attcgcccgg ctcgagcagg agcgcgaggt cgaaatccgc cgcgccgccc
     7381 agaatgccga ggtggcacag cagcagtccg agcggcagcg tgaggcggat gaagcgcgga
     7441 tcaccgccaa gcgccaggtc gacagcgccc agatcgaagc caaccgggtc gtcgagcagg
     7501 ccaagatcac ccaggcccag tcgctcgaaa tcgcccgcca ggagcagcag atcgcggtcc
     7561 agaacaagag ccgcgaggaa agccaggcca aggccgaggc cgacaccgcc cgcgccgccg
     7621 ccgtggccgc ggaggagaag gtcggcaccg cccgcgagac cgagattgcc gaacgcgtga
     7681 aaaggatcga gctgatcgac gccagccgcg aagccgagcg cgccgcgatc ggcatcaagg
     7741 tccaggccga ggccgccaag caggccgccg ccgaccatgc cgaagcgacg cggctcggcg
     7801 ccgagggcga ggccgaagcc gagaagcttc gcgccgaagc ctcgcgcgtc cgcttcgagg
     7861 tcgaggcggc cggccagcgc gcgatcaacg aggcggcgaa cctgctctcc tccgaccaga
     7921 tctcgctgca gaccaagatg gctttgctga aggtgctgcc cgaggtggtg cgcgaggcgg
     7981 ccaagccgat ggaggcgatc gattcgatca aaatcatcca ggtcgacggc ctcaccggtg
     8041 ccggtgcggg ctccggtccc ggcggtgccg gcgtcccgtc cggcgacggc aatctcgcct
     8101 ccagcgccgt cgccgcggcc ctgcgctacc gcgcccaggc cccgatcgtc gacggcctga
     8161 tgaaagagct cggcttcgac ggctccaccc tggagaacct cgtcgccggc gcggcggcaa
     8221 agcccgctga cgaacccgcg gtcacggtcg tcaccacccc ccggaagtag gagcccgctc
     8281 aattcctccc cggcttttgc cggggagggg gaccggcgaa gccggtggag gggcggcgcg
     8341 ccagcgcctc tcctcactcc caaaccttca cccgctcctc cgccggcaaa taaagcttgt
     8401 gccccggctt caccccgaac gcgtcgtacc aggcgtcgtg gttgcgcacc gaccacgtcc
     8461 gccacttgct gggcgaatgc acccccgaca gcaccgcctt ccgcaattcc gcctcgcgga
     8521 acgccacctt gttgacctgc gcccggccca tgaagaagcg ctcggccccg gtgaagccgt
     8581 cgatcaccgg cgcgggcttg ccgcccagcg acagttgata cgcgtcccag gcgatggcga
     8641 gcccggcatt gtcggcgata ttctcgccca aagtctgcgc gccgcgcacg ttgagcccgg
     8701 gcagcgcctc ataggcatca tattgggcga ccagcttctg cgcccgctcc ttgaagcggg
     8761 cgagatcctg gtcggtccac catttcacca gccgcccctc gggatcgaac ttcgatccct
     8821 ggtcgtcgaa atggtggctg atctcgtgcc cgatcacata gccgatcgcc ccgtaattga
     8881 ccgccgggtc ggcgcgcgga tcgaagtgcg gcgcctgcaa atagccggcc gggaacacga
     8941 tctggttgtt ctggaagctc gcataagcgt tggcggtgat cggcatcatc caccattcgc
     9001 ggcggtcgac cggccggtcg atgcgggcaa ggtcgcgctg ccaggcgaag cgggcggcgg
     9061 ccttgagatt ggcataggga tcgccgcgcg ccacccgcag cccctcatag ctgcgccagg
     9121 tgtcggggcg gccgatcatc ggcgtgaagg cggcgagctt ggcccgcgcc ttgaccttgg
     9181 tctcgggcgc catccagctc agattggcga gccgccggtc catcgcggcg agcacgttct
     9241 tgacgaggtc gtccatcgcc gccttggtct cggccgggaa gtggcgctcg atatagggcc
     9301 gcgacacggc gtcggtcatc gcggtggcgg tgagctccac cgcctgcagc cagcgcgccg
     9361 gcgcctcggt cgcgccgccg agcgtgccgc ccgaaaaggc gaattcctcg cgcgcgaacg
     9421 cggccggcag gaccagcgcg cggtccttgg cgaaccggat cgccagccag tccttcagca
     9481 ccggcagcgg cgtcgccccg aacagcttgg ccatcgccga gatcgccgtg gtctcgccga
     9541 cgatataggc cggccgccgc gccagcccgg cgccgcccaa aaaggcgtcc cagtcgaagc
     9601 ccggcgcgcg cgaccgcagc tcggccaaag tccaggggtt ataggtcttg tcggcgtcgc
     9661 gcgcatcggc gagcggccaa tgcgcctcgg cgatcccgcg ctcgaactga tagaccgccg
     9721 ccgcccgctg cgccgcctcc gccttggcga cgccggtcag ggccagcatc gcggcgaggt
     9781 ggccatgata agcggtctgg gtcgccgccg cgtcgccatc ctctttgaga taataatcgc
     9841 ggctcggcat gccgaggccg ccctggccga tccccggcac gtagcggttc ggctccttgc
     9901 tgtcctggcc gatgccgagc ccgaacggcg atgggaagta acgcggcatg cgcccgagcg
     9961 gcggcgtccc ctgccattcg cgcaccagcc cgcccatcaa ggcggccagc gccgaatgcg
    10021 aggcggcggc gcggatcggc ttcaggtcgg ccagcaaggg cgcggcgccg cgcttctcga
    10081 tcgcggcctg gtccatcagc gacgcgtaat aatcggcgat gcgccggtcg tcgcccgcga
    10141 ggctcccgcg ccggccatta tattcgtcga ggatcgcgcg gacctgcgac atggacaggt
    10201 cgttcagccg cgccacctgg ctcagcgccc cgcgatcggc cggtatctcc accgtccgcg
    10261 cccagcgccc gttgacgtag gaccagaaat cgtcgcccgg gtgcaccgcc cggtccatcg
    10321 aatcgaactc caccccgaac gtcccccacg ccggcttccc cggggcaggc tgggcggcgg
    10381 gtggctgctg ggcgggcgtg gcagcggcca cggtgagcgc gagaatcgaa gcaattccga
    10441 gaatggcggt ccgcatggtg tctcccttgt ttcggcaagt ctgccgcgcc cacaccggat
    10501 cggcaagcgc ccctaaattc ctcccctgca tttgcagggg agggggaccg cgcgaagcgc
    10561 ggtggagggg cccgcgcgaa gcgcgtctcg gtggatggac caggaggcga acccgtcggg
    10621 gggccgggca aagcgagcag cgccgcgccg cccctccacc accggctgcg ccggcggtcc
    10681 ccctccccgt tccggggagg agcaatctcg ctcatgctga ggggggactg agcgaagtcg
    10741 aaggcccgtc tcgaagcacg ctccccccta ccgccgcgaa aaccccgcca gcacctcgca
    10801 cacccagcgg ccggccgtca gcgccgtcac ctccccgaca tccagcgcag gatcgaactc
    10861 ggtcagatcg accagcttca cctgccgctg ccgcccgaga tagcgcgccg cctcgaagaa
    10921 catgtcggcc ggcatcccgc ccggccgcgc gcccggcgcg ccggggcatt gcgcgcggtc
    10981 gatcacgtcg atgtcgaaat cgaccatcac cgcgtccaca tgcccgagcc ggcccagcgc
    11041 ctcgtcgatg accgcgagga tgccgcggcg cttgcagtcc gcgatcgtat agacgccgat
    11101 gccggcgccc cgcgcgatgc cgtgcatccg cgcggtattg gcgaacggcg cgagcccgat
    11161 ctggcagatg ttgccgcccg gcatccggtc ttcgagcagc gcccggatcg gattgccgtt
    11221 caagagcccc tcgtcggtgt cgcgaaggtc gaaatgcgca tcgagcgtga tcaggccgac
    11281 ccggccgagc ggcaggccga gcccgtgcgc ggccgggcgg gtgatcgcat tatgcccgcc
    11341 gagcagcaag gtgagcgggt gcgcggcggt gcagcccgct accgcgcggc cgatcggtgc
    11401 gaacccctcc tccggcgtcc gcccctcgac cgcgacgtcg ccggcgtcgt agattcggag
    11461 cccgagctcc tgcccggtct cgacatcgta gcggctgaag cggcgcagcg tctcgcggat
    11521 cacccccggc gcgaggtcgt agcggccggg cgtgaccgac ttggcctcca tcggcgcccc
    11581 gagcaaggcg acgtcgcccg gtccttggga gacgatcagt tcgctgaggc gcggccaggc
    11641 ggaagtcggg ttcatggctt tgccttacac cggcttcgtg ccaaagggcg agccatgtgg
    11701 gaccgcctcc tcaccgactg caacatcgcc accatggacc cggccgtgcc gggcgcgttc
    11761 ggcgcgatcg agaatggcgc gatcggcatc gccgacggcc gcatcgtgcg ggtcggccgg
    11821 cgcaccgagc ttgccggcta ccgggcgcgc gaagtggtgc cgctgggcgg cgcgtgggtc
    11881 acgccgggcc tcgtcgattg ccacacccat ctcgtcttcg gcggcaaccg ctcggccgaa
    11941 tatgagatgc ggctcgaagg cgcgagctac gaggagatcg cccgcgccgg cggcggcatc
    12001 ggcgcgaccg tcaaggcgac tcgcgcggcc tcgatcgaag ccctggtgcg ggcggcgcgg
    12061 ccgcggctgc aggcgatgat gcgcggcggc gtcaccacca tcgagatcaa gtcgggctac
    12121 ggcctcaaca ccgacggcga attgaagatg ctcgaagcgg tgcgcgcgct cggcgacagc
    12181 gaggccgtgc gggtggtgcc gaccctgctc gcgctccatg cgctgcccgc cgacttcaag
    12241 gatcggcgcg ccgatttcgt gcggatggcg gtcgacgaga tgctccccgc cgccgccgcg
    12301 cgcggcctgg cgcaggcggt cgacgccttc gccgagggga tcggcttcac ggtcgatgag
    12361 gtgcgcgcct tgttcgcggc ggcgcgccgc cacggcctgc cggtgaagct ccacgccgag
    12421 cagctcagca atttgagcgg cgcggcgatg gccgccgaat atggcgcgct ttcggccgac
    12481 catctcgagc atgtcgacga ggccggcgtc gccgcgatgg cggcggccgg gatggtggcg
    12541 gtgctgctgc ccggcgcttt ctacgcgctg tcggagacga agaagccgcc ggtcgacctg
    12601 ctccgcgccc acggcgtgcc gatggcggtc gcgaccgact gcaaccccgg cacctcgccg
    12661 gtgctgtcgc cgaccttgat gctgagcatg gcctgcacct tgttcggcct cacccccgag
    12721 gaagcgctgg ccggcatgac ccgcgagggc gcccgcgcgc tcggcctcca ggacagtctc
    12781 ggcacgatca gcaccggcaa ggccgccgac ctctgcgtct ggcgcatcgg ccgccccgcc
    12841 gagcttagct actggatcgg tctccccggc cccgaacgcc gcatcgtcgg gggtgtggac
    12901 gcctagcgtg gctcccaccc cgttcgtccc gagcgaagtc gagggaccct ttcgagcgca
    12961 gccgagaagg caaggcctcg ccccggctcg gcggggtgtc tcgacttcgc tcgacacgaa
    13021 cggctagggg gttcccatga acagacgcga caacagccgc aatatccgcg cgcggaccgg
    13081 acccgagatc aaggccagga gctggtcgac cgaggccgcg gtccggatgc tgatgaacaa
    13141 tctcgatccc gacgtcgccg aggcgcccga ggagctggtc gtctatggcg gcatcggccg
    13201 cgccgcgcgc gactgggaga gcttcgaccg catcgtcgag acgctcgagc ggctcggcga
    13261 ggatgagact ttgctcgtcc aatcgggcaa gccggtcggc gtcttccgca cccatgagga
    13321 cgcgccgcgc gtgctgctcg ccaatagcaa cctggtgccg aaatgggcga cctgggagca
    13381 tttcaacgcg ctcgatcgca agggcctgat gatgtacggc cagatgacgg ccgggtcgtg
    13441 gatctacatc ggcacccagg gcatcgtcca aggcacgtac gagacgttcg cggagatggg
    13501 acgccagcat tatggcggcg accttgccgg caagtggatc ctgaccgcgg ggctgggcgg
    13561 catgggcggt gcgcaaccgc tcgctgccgt gatggccggc gcacattgtc tggccataga
    13621 atgccaacaa agccgcattg agaaacgcct cgcaacccgt tatttagaca aggcaaccgc
    13681 cagtctcgac gaagccttgc agatgctctc cgaggcaact tcgccaacat ccgtcggcct
    13741 cctgggcaac gccgccgaga tccttcccga aatgctgaag cgcggcatcc gcccccacgc
    13801 cctcaccgac cagacgtccg cccacgaccc ggtcaatggc tatttgccga tcggctggac
    13861 cgtcgagcaa tggatcgagc gccgcgaacg cgaccccaaa gcggtcgagc atgccgcccg
    13921 cgcctcgatg gccgtccatg tcgaagcgat gctcgcctat cgcgaaatgg gcatccccac
    13981 cttcgattat ggcaacaata tccgccaggt ggcccgggac gagggcgtcg aacatgcctt
    14041 cgacttccct ggctttgttc ccgcctatgt gcgtccgctc ttctgccgcg gcatcgggcc
    14101 gttccgctgg gtcgctttgt cgggcgatcc cgaggacatc taccgcaccg acgccaaggt
    14161 gaaagagctg atccccgacg atgcccatct ccaccgctgg ctcgacatgg cgcgggagcg
    14221 gatcgccttc cagggccttc ccgcccgcat ctgctgggtc ggcctcggcc agcgccaccg
    14281 gctcggcctc gccttcaacg aaatggtcgc ccgcggcgaa ctaaaggcgc cgatcgtcat
    14341 cggccgcgac catctcgatt ccggctcggt cgccagcccc aaccgcgaga ccgaggcgat
    14401 gaaggacggc tccgacgcgg tctcggactg gccgctcctc aacgcgctcc tcaacaccgc
    14461 ctcgggcgcg acctgggtct cgctccacca tggcggcggg gtcggcatgg gctattcgca
    14521 gcatgccggc atggtgatcg tcgccgacgg cacggcagcg gccgcccgcc gcctcgagcg
    14581 tgtgctgtgg aacgaccccg gcaccggcgt catgcgccac gccgacgctg gctacgacat
    14641 cgccatcgat tgcgcgcgcg agcaggacct cgatttgccg atggtggggc ggtgacgagc
    14701 ccgatcaccc tgcgccccgg ctccgtcacc ctcgccgaac tgcgcgcgat ctgggccggc
    14761 gctcccgtcg cgctcgccga cgaggcctgg cgcgcggtcg aggcggccgc cgagagcgtc
    14821 gcccgcatcg tcgcctcggg ccggaccgtc tacggggtca ataccggctt cggtctgctc
    14881 gcccagaccc gcattcccga cgaccggctg ctcgacctcc agcgcaacct catcctatcg
    14941 cacagctgcg gcctcggcga cgcgctcgac ccgcgcgtcg tgcgcctgat catggtgctg
    15001 aaggtcattg gcctcgcacg cggccattcc ggcgtgacgc gggcgttggt cgaacgcctg
    15061 ctcgcgctga tcgaggccga ggcctttccg gtggtgcccg cgcaaggctc ggtcggcgcc
    15121 tcgggcgatc tcgccccgct cgcccacctc agcgccgcgc tgatcggcga aggcgccatc
    15181 ctgctcggag gcgagcgcat gccggcggcg gacgcgctgg ggaagctcgg gctggagccg
    15241 ctggtgctgg gcccgaagga gggcctggcg ctgatcaacg gcacccaggt ctcgaccgcc
    15301 gtcgcgctcg atgccttgtt cgccggcgaa cgcgtgttcg gatcggcggt cgtcgccggc
    15361 gcgatgtcgg tcgacgccct gaagggcacc gacgtcgcct tcgatccgcg catccatgag
    15421 gcgcgcggcc aacccgggca gatagcggtc gcggccgcgc tgaagcggct gctcggtgga
    15481 agcgcgatcc gcgagtcgca cgccggctgc ccgcgcgtgc aggatcccta cagcttccgc
    15541 tgccagcctc aggtgatggg tgcggcgctc gacctgatgc gcagcgtcgc ccggacgctg
    15601 gagatcgaag ccaatgccgt caccgacaat ccggtcatat tcggcgacga tgcggtgtcg
    15661 ggcgggaatt tccacgccga gccggtcgcc ttcgccgccg acatgctggc gatggcgctg
    15721 tgcgaggtcg gctcgatctc ggaacggcgc accgccgcgc tggtcgatcc gaagatgagc
    15781 ggccttccgc ctttcctcgt ccaggacagc ggcgtcaatt cgggcttcat gatcgcacag
    15841 gtgacggcgg cggctttggt ctcggagaat aaaagcctcg ctttccccgc cagcgtcgat
    15901 tcgatcccca cttcggctgg ccaggaagac catgtatcga tggcgactca cggggcggtg
    15961 aaggcgcggc ggatcgccgg caatgccgcc ggcgtgatcg gcatcgaact gctcgccgcc
    16021 gcgcaggggc tcgacttcca cgccccgctg cgcacctcgc cggcgctggt cccggtgcac
    16081 caggcgatcc gcgccgaaat tccccattat gcttccgacc ggtatctcgc cgacgagctc
    16141 gcctgggcga agcgggaggt gctggagggg cggctgggcg aagagctccg ggcggagctg
    16201 ttcaagtgat ccccgccccg gcatgattga cctcgatcag gccccggccc aagccgaact
    16261 tgccacggag gtctgcgtaa tcggcgccgg cgccgccggg atcagcgtga cgcgcaagct
    16321 gttggcggcg gggcatgagg tggtgctgct cgaaagcggc ggcctcgact atgaggccga
    16381 cgtcgccgag ctcaacacgg gcgaaaacgt cggcgaggat tattacgacc tcgaagacag
    16441 ccgcctgcgc ttcttcggcg gcaccacggc gatctggggc ggccgctgcg cggagctgga
    16501 cccgatcgat ctcgaaaagc gcgactatgt gccgcattcg ggctggccga tcg
//
LOCUS       DASVRK010000275         4572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_431206, whole genome shotgun sequence.
ACCESSION   DASVRK010000275 DASVRK010000000
VERSION     DASVRK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431206"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /gene="ruvX"
                     /locus_tag="VF589_11120"
     CDS             complement(<1..237)
                     /gene="ruvX"
                     /locus_tag="VF589_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX8258174.1"
                     /translation="MITASAGEFREALPMGGRLLGLDVGTKTIGIALCDAGWTIATAA
                     ELIRRGKFAADLTRLRGIVAAQRVAGIVVGLPLNL"
     gene            complement(234..1343)
                     /locus_tag="VF589_11125"
     CDS             complement(234..1343)
                     /locus_tag="VF589_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX8258175.1"
                     /translation="MARRFLWVVAGLTMLLLLAALGYRIFQRQWMELYFIPSAKFEEV
                     PMPDGPSYADASLWIARPDIADNPALWTPAGVARGGRNAASLFFIHPTSYLEGTRWNA
                     PVDDVESQLRAELFVRHQASPFNQVAAIWAPKYRQATFGAFLTTQEDALKALDYAYRD
                     VLAAFDAFVAQAPKDRPIILAGHSQGSRHLAQLLQDRVAGKPLARRIAAAYVIGWPIS
                     TTADLPSLGLPACERAGQAGCVLSWQSFAEPADPEMVTDVYDASRAPNGRPRAGSPML
                     CTNPLTGNRGGEAPAAANLGTLIPNAALTEGALTPALVPARCDAGGFLLIGETPPKLP
                     PYVLPGNNYHVFDYALFWANVRADAEARLRSFAAR"
     gene            1500..1787
                     /gene="gatC"
                     /locus_tag="VF589_11130"
     CDS             1500..1787
                     /gene="gatC"
                     /locus_tag="VF589_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX8258176.1"
                     /translation="MSVDQAIVRRIAKLARIAVTDAEADALVPELNNILGWIEQLQEV
                     DVTGVEPMTAVIPNTLRLRPDVVTDGGKREAVLRNAPLAEHGFFAVPKVIE"
     gene            1930..3411
                     /gene="gatA"
                     /locus_tag="VF589_11135"
     CDS             1930..3411
                     /gene="gatA"
                     /locus_tag="VF589_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX8258177.1"
                     /translation="MTALTDLGVAQIRDGFRDGAFTARQVAEAFNAAVEKARGLNAFV
                     IATPDHALAAADQADRDRAAGNLRPLSGVPLGIKDLFATEGVQTTAGSNMLGGFAPAY
                     ESTVSRHLFEAGAGMLGKLNMDEFAMGSSNETSAYGPVISPWRRSDGGNAALTPGGSS
                     GGSAAAVAARIAPGVTGTDTGGSIRQPAAFTGITGIKPTYGRCSRWGIVAFASSLDQA
                     GPMARDVRDCAILLEAMAGFDPRDATSLELPVPHWEAGLSSDIRGKRIGIPKEYRIEG
                     VPDEIATVWDKGIELMRDAGAEIVEVSLPHTRYALPTYYIIAPAEASSNLARYDGVRY
                     GRRVAPDRGNLADMYEATRAAGFGAEVKRRIMIGTYVLSAGFYDAYFNQAQKVRALIA
                     RDFERVWSECDLLLTPTAPSAAFALGDKSDDPIAMYLNDVFAVPASLAGLPAISVPGG
                     LNDQGLPLGLQLIGKPLDEQSVLNAALAIEERAGFTARPKAWW"
     gene            3405..3689
                     /locus_tag="VF589_11140"
     CDS             3405..3689
                     /locus_tag="VF589_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258178.1"
                     /translation="MVMLGETPWNQRDFTFGLAGFLAGIHALRILAEKGIATAEDIEV
                     SLEGIRATLRNLPEGLVSDTQRAGIDSMLATLHAAALNAAAPNTEEPSDG"
     gene            3682..>4572
                     /gene="gatB"
                     /locus_tag="VF589_11145"
     CDS             3682..>4572
                     /gene="gatB"
                     /locus_tag="VF589_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX8258179.1"
                     /translation="MADASAAYRIHGATGEWEVVIGLEIHAQITSKAKLFSGASTEFG
                     AEPNSQVSLVDAAMPGMLPVPNAECIRQAVRTGMALEARINAWSRFDRKNYFYADLPQ
                     GYQISQLYHPIVGEGSIAIEPAEGVVKTVGIERIHIEQDAGKLMHDQHPSFSYVDLNR
                     CGVALMEIVSRPDMASPEEAGAYVRKLRSILRYVGSCDGNMEQGSMRADVNVSVRRPG
                     EELGTRTETKNVNSIRFVMQAIEHEAQRQVEVLESGGRIEQETRLFNPDTGTTRTMRS
                     KEDAHDYRYFPDPDLLPLELD"
BASE COUNT          774 a         1676 c         1435 g          687 t
ORIGIN      
        1 gaggttgagc ggcagcccga ccacgatccc cgccacacgc tgcgcggcga cgatccccct
       61 cagccgcgtg aggtccgccg cgaacttgcc gcgccggatc agctcggccg cggtcgcgat
      121 cgtccagccg gcgtcgcaca aggcgatccc gatcgtcttg gtccccacgt cgagcccgag
      181 cagccgcccg cccatcggca gcgcctcacg aaattcgccc gcgctcgccg taatcatcgc
      241 gccgcgaagc tccgcagccg cgcttcggcg tcggcgcgca cgttcgccca gaacagggca
      301 taatcgaaca catgataatt attgcccggc agcacatagg gcgggagctt gggcggggtc
      361 tcgccgatca gcaggaagcc gccggcgtcg cagcgggcgg gtacgagggc aggggtcagc
      421 gccccttcgg tcagggccgc attcgggatc aacgtcccga gattggccgc ggccggcgcc
      481 tcgccgccgc gattgccggt gagcggattg gtgcacagca tcggcgatcc ggcccgcggc
      541 cgcccgttcg gcgcccgcga cgcatcatag acgtcggtca ccatctccgg atcggcgggc
      601 tcggcgaagc tctgccacga aagcacgcag ccggcctggc cggcgcgctc gcaggcgggc
      661 aggccgagcg agggcagatc ggcggtggtc gagatcggcc agccgatcac ataggcggcg
      721 gcgatccgcc gggcgagcgg cttgcccgcc acccggtcct gcagcaattg cgcgagatgg
      781 cggctgccct ggctgtggcc ggcgaggatg atcggccggt ccttcggcgc ctgggcgacg
      841 aacgcgtcga acgccgccag cacgtcgcga taggcataat cgagcgcctt cagcgcatcc
      901 tcctgcgtgg tgagaaacgc gccgaacgtc gcctgccggt atttgggcgc ccagatcgcg
      961 gcgacctggt tgaacgggct cgcctggtgg cggacgaaca attccgcccg caactgcgat
     1021 tcgacatcgt ccaccggcgc gttccagcgc gtcccttcga gataggatgt cgggtggatg
     1081 aagaacaagg acgccgcgtt ccgcccgccg cgcgccacgc ccgccggcgt ccacagcgcc
     1141 ggattgtccg caatatcggg ccgcgcgatc cacaacgacg cgtcggcata gctcggcccg
     1201 tccggcatcg gcacctcctc gaacttggcc gaggggatga agtaaagctc catccattgc
     1261 cgctggaaga tgcggtagcc gagcgcggcc aacagcagca gcattgtcag ccccgccacc
     1321 acccacagaa aacgccgcgc caacatcgtc tcactccttt gcgcgccccc aagcgcatgc
     1381 cggccgcgct tgcaagccgc gctttcgccc acaccgttcc gctccgcgct ttcccgccga
     1441 cgccttgaag ccgtgccacg ccttgaagcc ataagagccg ggtgctagcg gccgctccca
     1501 tgtccgtaga ccaggctatc gtccgccgca tcgccaagct cgcccgcatc gccgtcaccg
     1561 acgccgaagc ggacgcgctc gtgcccgagc tcaacaacat cctcggctgg atcgagcaat
     1621 tgcaggaagt cgacgtcacc ggcgtcgagc cgatgaccgc cgtcatcccc aacaccttgc
     1681 gcctgcgccc ggacgtcgtc accgacggcg gcaagcgcga agcggtgctc aggaacgccc
     1741 cgctggccga gcacggcttt ttcgccgtgc cgaaagtgat cgagtgatgg cggcgggaat
     1801 ggtgggggcg cggaggccag cctttttcgt cattccagcg caagctggaa tctcccttct
     1861 taaaggcaaa gagattccag cttgcgctgg aatgacgagg ggctccgctg gaatgacggg
     1921 gaacaccctg tgaccgccct caccgacctc ggcgtcgccc agatccgcga cggcttccgc
     1981 gacggcgcct tcaccgcccg ccaagtcgcc gaggccttca acgccgccgt cgaaaaggcg
     2041 agggggctca acgccttcgt gatcgcgacg cccgaccacg cactcgccgc cgccgaccag
     2101 gccgaccggg accgcgccgc cggcaatttg cgcccgctct ccggagtccc gctcggcatc
     2161 aaggacctgt tcgccaccga gggcgtccag accaccgccg gcagtaatat gctcggcggc
     2221 ttcgcgcccg cctacgaatc caccgtcagc cgccatttgt tcgaggccgg cgccggcatg
     2281 ctcggcaaat tgaacatgga cgagttcgcg atgggctcgt ccaacgagac cagcgcctat
     2341 ggccccgtaa tctcgccctg gcggcgcagc gacggcggca atgccgcgct gaccccgggc
     2401 ggctcgtcgg gcggctcggc cgcggcggtc gccgcccgca tcgcccccgg cgtcaccggc
     2461 accgacactg gcggctcgat ccgccagccc gcagccttca ccggcatcac cggcatcaag
     2521 ccgacctacg gccgctgctc gcgctggggc atcgtcgcct tcgcctcctc gctcgaccag
     2581 gccgggccga tggcgcgcga cgtccgcgat tgcgcgatcc tgctcgaggc gatggccggc
     2641 ttcgacccgc gcgacgcgac ctcgctggag ctgcccgtgc cgcattggga ggccggactt
     2701 tccagcgata tcaggggcaa gcgcatcggc attcccaagg aatatcggat cgagggcgtg
     2761 ccggacgaga tcgctaccgt ctgggacaag ggcatcgaac tgatgcgcga tgccggcgcc
     2821 gagatcgtcg aagtctcgct cccccacaca cgctacgcgc tcccgaccta ctacatcatc
     2881 gcccccgccg aagcctcgtc caacctcgcc cgctatgacg gcgtccgcta cggccggcgt
     2941 gtcgccccgg atcgcggcaa ccttgccgac atgtatgagg caacccgcgc cgccggcttc
     3001 ggcgccgagg tcaagcgccg catcatgatc ggcacttacg ttttatccgc aggcttttac
     3061 gacgcttatt tcaatcaggc tcagaaggtg cgcgcgctca tcgcccgcga tttcgagcgg
     3121 gtctggtccg aatgcgacct gctgctcacc ccgaccgcgc cctcggccgc cttcgcgctg
     3181 ggcgacaagt ccgacgatcc catcgccatg tacctgaacg acgtcttcgc cgttcccgcc
     3241 agcctcgccg gcctgccggc catctcggtc cccggcggcc tcaacgatca gggcctgccg
     3301 ctcggcctcc agctgatcgg caagccgctc gacgagcaaa gcgtgctcaa cgcagccctc
     3361 gcgatcgagg aacgcgccgg cttcaccgcc cgcccgaagg cctggtggtg atgctcggcg
     3421 agaccccctg gaaccagcgc gacttcacct tcggcctggc cggcttcctc gccggcatcc
     3481 acgcgctccg catcctcgcc gaaaagggca tcgccaccgc cgaggatatc gaagtgtcgc
     3541 tcgaaggcat tcgagcaacc ctccgcaacc tgccggaagg cctggtttcc gacacccagc
     3601 gcgccggcat cgattcgatg ctcgccacgc tccacgccgc cgcgctcaat gccgccgctc
     3661 ccaacaccga ggagccatcc gatggctgac gcttccgccg cttaccgcat ccacggcgcc
     3721 accggagagt gggaggtcgt gatcgggctc gagatccacg cccagatcac ctccaaagcc
     3781 aagctcttct ccggcgcctc gaccgaattc ggcgccgagc ccaacagcca ggtcagcctg
     3841 gtcgacgcgg ccatgccggg catgctcccg gtcccgaacg ccgaatgcat ccgccaggcg
     3901 gtccgcaccg gcatggcgct ggaggcgcgg atcaacgcct ggtcgcgctt cgaccgcaag
     3961 aattatttct acgcggacct gccgcagggc tatcagattt cccagcttta tcatccgatt
     4021 gtcggcgaag gctcgatcgc gatcgaaccc gctgaggggg tggtgaagac ggtcggcatc
     4081 gagcgcatcc atatcgagca ggatgccggc aagctgatgc acgatcagca tcccagcttc
     4141 tcctacgtcg atctcaatcg ctgcggcgtc gccctgatgg agatcgtctc gcgccccgac
     4201 atggcgagcc cggaggaagc gggcgcctac gtgcgcaagc tgcgctccat cctgcggtac
     4261 gtcggctcgt gcgacggaaa catggagcag ggctcgatgc gtgccgacgt caacgtcagc
     4321 gtgcgtcggc cgggtgaaga actcggcacg cgaacggaga ccaagaacgt caattcgatc
     4381 cgcttcgtca tgcaggcgat cgaacatgag gcgcagcggc aggtcgaggt gctggagagc
     4441 ggtggcagaa ttgagcagga aacccgcctg ttcaatccgg acaccggcac gacgcggacg
     4501 atgcggtcca aggaggatgc gcacgattac cgctacttcc ccgatcccga cctgctgccg
     4561 ctggagttgg ac
//
LOCUS       DASVRK010000276         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_158731, whole genome shotgun sequence.
ACCESSION   DASVRK010000276 DASVRK010000000
VERSION     DASVRK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158731"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..589
                     /locus_tag="VF589_11150"
     CDS             29..589
                     /locus_tag="VF589_11150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8258180.1"
                     /translation="MTPFDKAQDEREPVETVWEGKFIAAKRIGKWEFVSRTRGVSAAV
                     ILAVHEGHVLLVEQHRAPLGAMCVEPPAGLVGDEEEGEAIETAAIRELEEETGYRAER
                     MVALGRFHASPGMSSEGFTLLRAEGLTKVGEGGGVAGEDIIVHRVPLGEVPAFVAAKR
                     AEGAAMDVKMLILLAGPMLAGLESRP"
     gene            complement(716..994)
                     /locus_tag="VF589_11155"
     CDS             complement(716..994)
                     /locus_tag="VF589_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEX8258181.1"
                     /translation="MAARIFQESRNAMQSGTAREGRWLLEFESHARRSPDPLTGWSGG
                     ADTQAQVSINFDSLEAATAYAEREGIDYHVLPPTTRKIRLQSYADNFR"
     gene            complement(1022..3547)
                     /gene="hrpB"
                     /locus_tag="VF589_11160"
     CDS             complement(1022..3547)
                     /gene="hrpB"
                     /locus_tag="VF589_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEX8258182.1"
                     /translation="MSGGVNGISLPLPIDAVLPDLLATLRAGSNAVLVAPPGAGKTTA
                     VAPALLDEAWCTGEILLLSPRRLAARAAAERMAELAGERVGDTIGYATRLDTKRSART
                     RILVLTEGIFLSRIQADPELAGVSAVLFDEVHERSLDSDFGLALALDAQAALRPDLKL
                     VVMSATLEGDRFARLMGGAPVIESHGRSHPIDLRYLGRAAERRVEDEMATAIRRVLGE
                     AEGSLLAFLPGVAEIERTAERLQGLPAMVDLHRLHGTLDPAEQRAAIRAAPAGRRKIV
                     LATSIAETSLTLDGVRIVVDSGLARRPRYDRGAGITRLVTERASQASVTQRAGRAGRQ
                     APGTVYRLWEEAATASLPRFDPPEILEADLSALLLDCAIWGVADPRALGWIDPPPAAA
                     VDEARRRLTALGAIDAAGRPTAHGKAIARLPLPPRLAHMLIEAEARGWGATAADAAVL
                     LSERGLGGNDSDLELRLKRWRGDRGPRAEGARGLARSWLRMTRSPGKAGVQPEALAAL
                     RLPTGDAVAGCIALAFPDRVSKRRDASGENWLSVGGRGFRLDPTSPLARETWLAVAEV
                     SGTAAGARILSAAALSEEAVQSLFGDRIETGTHMTFDPATGTVRASRGRRLGAITLAQ
                     GQDGKASPEEVAAALIEGVRTHGLHLLPWSEAAISLRKRAAFARRFDESLPDLSDEAL
                     TATLDDWLPPIVAGKRALRDVDPSALSGALDALLGWEGLKAVDRIAPHSFETPAGSSH
                     AIDYEAEAGPTVTARPQAFFGLARHPMIGDGRVPLVLSLTSPAGRPIQTTRDLPGFWA
                     GSWADVAREMRGRYPKHPWPDDPAQADATLRTKKASARSGRAV"
     gene            complement(3665..>4095)
                     /locus_tag="VF589_11165"
     CDS             complement(3665..>4095)
                     /locus_tag="VF589_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX8258183.1"
                     /translation="AVIAERSEGEEAALDAYGRNLGIAFQLIDDAIDYASDAGTMGKG
                     VGDDFRDGKVTLPVILAVARGSEAERAFWRDAMEGRRVSDEDLAFATGLLSAHGAIDD
                     TIGRARTYGQRAIDALGPFPAGKAKAALVEAVEFAISRAY"
BASE COUNT          573 a         1379 c         1547 g          596 t
ORIGIN      
        1 acgagcttcc cgaccggctg atcgagctgt gacccccttc gacaaggctc aggatgagcg
       61 ggaacccgtc gagacggtct gggaaggcaa gttcatcgcc gccaagcgga ttggcaaatg
      121 ggagttcgtc tcccgcaccc gcggcgtcag cgccgccgtg atcctggcgg tgcatgaggg
      181 ccatgtgctg ctggtcgagc agcatcgcgc gccgctcggc gcgatgtgcg tcgagcctcc
      241 cgccggcctg gtcggcgacg aggaggaagg tgaggcgatc gagaccgccg ccattcgcga
      301 gctcgaggag gagaccggct accgcgccga acggatggtc gcgctggggc ggttccatgc
      361 ctcgccgggg atgtcgtcgg aagggttcac cctgctgcgg gccgaggggc tgaccaaggt
      421 cggcgagggc ggcggcgtcg ccggcgagga catcatcgtc caccgcgtgc cgctcggcga
      481 agtgccggcc ttcgtcgccg ccaagcgcgc cgagggcgcg gcgatggacg tcaagatgct
      541 gatcctgctg gccgggccga tgctggcggg gctggaaagc cgcccctaaa ctgttcgtgt
      601 cgagcgaagt tgagacaatt cttcgagcgt agcgagaacg gacgggaagg cctcggcttc
      661 gctcggcggg gtccctcgac ttcgctcggg atgatcggat cccgtgccgt tcgccctagc
      721 ggaaattgtc ggcgtaggat tgcagccgga tcttgcgggt cgtcggcggg aggacgtgat
      781 agtcgatgcc ctcgcgctcg gcataggccg tggccgcctc gaggctgtcg aagttgatcg
      841 agacctgcgc ctgggtgtcg gcgccgcccg accagccggt cagcggatcg ggactgcgac
      901 gggcgtggga ttcgaactcc agcaaccagc gtccctcccg ggccgtgccg gattgcatgg
      961 cgttcctcga ttcctggaag atgcgtgccg ccatccctgc ctcgatcctt gttcgcgcct
     1021 gctataccgc cctgcccgaa cgggcggaag ccttcttggt tctcagcgtc gcatccgcct
     1081 gcgccgggtc gtccggccac gggtgcttgg ggtagcggcc gcgcatctcg cgcgcgacgt
     1141 cggcccagct gccggcccag aagccgggaa ggtcgcgcgt ggtctggatc ggccgccccg
     1201 ccggcgaggt caggctgagc accaaaggca cgcggccgtc gccgatcatc gggtgacggg
     1261 cgaggccgaa gaacgcttga gggcgggcgg tgacggtcgg tcccgcttcg gcttcgtaat
     1321 cgatggcgtg gctgcttccg gccggggttt cgaagctgtg cggggcgatg cggtcgaccg
     1381 ccttcagccc ttcccagccg aggagggcgt cgagcgcgcc ggagagggcg gacggatcga
     1441 cgtcgcgaag cgcgcgcttg ccggcgacga ttgggggcag ccagtcgtcg agggtggcgg
     1501 tgagggcctc gtcggagagg tcgggcaggc tttcgtcgaa gcggcgggcg aaggcggcgc
     1561 gtttgcgcaa ggaaattgcg gcttcgctcc aggggaggag atggaggccg tgggtgcgga
     1621 cgccttcgat gagggcggcg gcgacttctt cgggcgaggc cttgccgtcc tgaccctgcg
     1681 ccagggtgat cgcgccgagg cggcggccgc ggctggcgcg gacggtgccc gtggcgggat
     1741 cgaaagtcat gtgcgtgccg gtttcgatgc ggtcgccaaa caggctttgc accgcttcct
     1801 cggaaagcgc ggcggctgag aggatgcggg cgccggcggc ggtgccggag acttcggcga
     1861 cggcgagcca ggtctcgcgg gcgagcggcg aggtgggatc gagccggaag ccgcggccgc
     1921 cgaccgaaag ccagttctcg ccggaagcgt cgcggcgttt cgaaacgcga tcgggaaagg
     1981 ccaaagcgat gcagccggcc acggcgtctc cggtcgggag tcgaagtgcg gcgagggctt
     2041 cgggctggac cccggctttc cccggggagc gggtcatccg gagccagctt ctcgccaggc
     2101 cccgcgcccc ttccgcccgc gggccgcggt cgccgcgcca gcgcttcagc ctcagctcga
     2161 gatcgctgtc gttgccgccg aggccgcgtt ccgacagcaa caccgccgca tcggccgcgg
     2221 tcgcgcccca gccgcgcgct tcggcttcga tcagcatatg cgcgaggcgc ggcggcagcg
     2281 gcagccgggc gatagccttg ccatgagcgg tcgggcggcc agccgcgtcg atcgcgccga
     2341 gcgcggtcag gcgccgccgc gcctcgtcga ccgcggccgc cggcggcggg tcgatccagc
     2401 ccagcgcgcg cgggtcggcg acgccccaga tcgcgcagtc gagcagcaag gcgctgagat
     2461 ccgcctccaa tatctccggc gggtcgaagc gcggcagcga ggccgtcgcc gcttcttccc
     2521 acagacggta gacggtgccc ggcgcctggc ggccggcgcg gcccgcgcgc tgcgtgaccg
     2581 acgcctggct ggcgcgttcg gtgacgaggc gggtgatgcc ggcgccgcgg tcgtagcgcg
     2641 gacggcgggc gaggccggaa tcgacgacga tgcggacgcc gtccagcgtc aggctggtct
     2701 ccgcgatcga ggtcgccagc acgattttcc gccgccctgc gggggccgcc cggatcgcgg
     2761 cgcgctgctc ggcggggtcg agcgtgccgt ggaggcggtg gaggtccacc atggcaggga
     2821 gcccctggag ccgctcggcg gtgcgttcga tctcggcgac gccgggcagg aaggcgagca
     2881 ggctgccctc ggcctcgccg agcacgcggc ggatcgccgt cgccatctca tcctcgaccc
     2941 gccgttccgc cgcgcgcccg agatagcgaa ggtcgatcgg atggctgcgg ccgtggcttt
     3001 cgatcaccgg cgcgccgccc atcaaacggg cgaagcggtc gccctccagc gtcgccgaca
     3061 tgacgacgag cttgaggtcc ggccgcagcg ccgcctgcgc gtcgagcgcg agcgcgagcc
     3121 cgaaatcgct gtcgaggctg cgctcatgga cttcgtcgaa caggacggcc gagacgccgg
     3181 caagctccgg atcggcctgg atgcggctga ggaaaatgcc ctccgtgagg accaggatgc
     3241 gggtgcgggc ggagcgcttg gtatcgagcc gcgtcgcata gccgatcgtg tcgccgaccc
     3301 tctcgcccgc cagctccgcc atccgctccg ccgcggcgcg cgcggccagc cggcgcgggg
     3361 ataggagcag gatctcgccc gtgcaccagg cttcgtccag cagcgccggc gcgaccgccg
     3421 tcgtcttgcc cgcgcccggg ggagcgacga gcacggcgtt gctgccggcg cgaagggtgg
     3481 ccagcaggtc cgggagcacg gcgtcgatgg gaaggggcaa ggaaattccg tttactccac
     3541 cgctcatccg ctgagcgtag tcgaagcggc gtctcgaagc accccgggcg aaggtccttc
     3601 gagaagggtc ttcgccaagc tcagcccctc ctcaggatga gcgattttcc cgatacccga
     3661 cggctcaata agcccgcgaa atcgcgaact ccaccgcctc caccagggcc gccttggcct
     3721 tgcccgccgg gaacgggccg agcgcgtcga tggcgcgctg gccgtaggtg cgggcgcggc
     3781 cgattgtgtc gtcgatcgcg ccgtgggcgc tgagcagccc ggtggcgaaa gcgagatcct
     3841 cgtcggaaac gcggcggcct tccatcgcgt cgcgccagaa ggcgcgctcc gcctcgctgc
     3901 cgcgggcgac ggcgaggatc accggcaaag tcaccttgcc gtcgcggaaa tcgtcgccca
     3961 cgcccttgcc catcgtgccg gcgtccgacg catagtcgat cgcatcgtcg atcagctgga
     4021 aggcgatgcc gaggttgcgg ccataggcgt cgagcgcggc ctcctcgcct tccgagcgct
     4081 cggcgatgac cgcgg
//
LOCUS       DASVRK010000277         8814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_367827, whole genome shotgun sequence.
ACCESSION   DASVRK010000277 DASVRK010000000
VERSION     DASVRK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367827"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VF589_11170"
     CDS             <1..401
                     /locus_tag="VF589_11170"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEX8258184.1"
                     /translation="STGRPLAQWQEQRSGGLAGEVELAPLILLPARQWVYARCDERFE
                     KMMSEEGLEEVRSLLERRLDPMLPVMRAIGVREIAGYLSGELSREKALAAGRTATRQY
                     AKRQYTWFSRQPPADWPRFADGVAAQWQLP"
     gene            411..1388
                     /gene="ilvC"
                     /locus_tag="VF589_11175"
     CDS             411..1388
                     /gene="ilvC"
                     /locus_tag="VF589_11175"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX8258185.1"
                     /translation="MHVYRDEDADLALIRGRRVAVVGYGNQGRAQALNLRDSDVPVTI
                     ALAEDSASRVRAREDGFDVMTAAEASAKADLVVMLAADELHTRVYAEEVEPNLAQGAA
                     LVFAHGLSIRFGLIEPRPDLDIFLVAPKGPGTALRREYEQGRGLIALFAVHQDKSGTA
                     NATALAYAAAIGSGRVGILETSFAEECEADLFNEQAVLWGAIPELIQAGFETLVDAGF
                     SPEIAYFECLTEVKLIADLIYERGIAGMREAISNTAEFGALKGGTRIVTAETRTEMRR
                     ILREVRGGGFVKDLVADAAAGYPLLRVSRAQARAHPIEAVGARLRELEP"
     gene            1385..1945
                     /locus_tag="VF589_11180"
     CDS             1385..1945
                     /locus_tag="VF589_11180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8258186.1"
                     /translation="MIFFKTPRLAIRRAREDDLEPLLLSWSDPAMTRYTGIRPDVRGF
                     LGQMIADMQVKQPGEIDPGGPWYQYIAERKADGALVGDLGVGFGIPGERQVELGYRIL
                     PGFHRQGYAREAVAGLIDYLIERHAIHRFVGIAAVPNIASTGLLRSLGFRQEGRFRQS
                     FLCHGEWLDDDYLALLASEWRGGASG"
     gene            complement(1942..2451)
                     /locus_tag="VF589_11185"
     CDS             complement(1942..2451)
                     /locus_tag="VF589_11185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8258187.1"
                     /translation="MGPEAGDRPPGAAKALRKPDVRYVPTPQPVVERMLELARVGKDD
                     LVYDLGSGDGRIPITAALRYGARGVGIDIDPKRIAESRANARKAGVTGRVSFRNEDLF
                     EADFGDATVVTLFLYPDLNRRLRPRLLAELKPGTRVVSYWHDMGNWKPERTVETERAS
                     IYLWTIPPR"
     gene            complement(2515..4296)
                     /gene="mutL"
                     /locus_tag="VF589_11190"
     CDS             complement(2515..4296)
                     /gene="mutL"
                     /locus_tag="VF589_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX8258188.1"
                     /translation="MSIRRLPEHLVNRIAAGEVVERPASALKELVENAIDAGATRIAI
                     ALSGGGIDRIEVVDDGCGMAPGDMALALERHATSKLPGDAIEAVATLGFRGEALPSIA
                     SVARLAIESRVRPSPGSGQAPADGWSRIIDNGRLEGEGPAALPPGTRITVADLFGKVP
                     ARRKFLRSARSEYAACLDVVKRLAMARPDIGFAFAHDGRRSLSLQPGEDRPARVAGLT
                     DRGLADNSVAVDYQRGPATLGGVAGLPTFNRGVADHQFLFVNGRPVKDRLLIGAVRAA
                     YQDLLARDRHPIVALFIELPGEEVDVNVHPAKTEVRFRDPANIRGLIVGGLRNALEAA
                     GHRSAQRPSASALGNWQAGPAAAPAQRPSFARPDFGQSSVWERHDAYQPAPAGRAEIA
                     AEPPPIAATFPLGVARGQVAATYIVAEAEDGLVLVDQHAAHERLVLERMRKAMANGGV
                     ARQSLLLPEVVELDEPACDRLEARADELAEMGLELERFGPRAMLVRATPALLGHGDVR
                     GLVTDLADELAAYDEALALKERLDHVAATMACHGSVRAGRVLSVAEMNALLREMEVTP
                     HSGQCNHGRPTWVKLGHADIEKLFGRR"
     gene            4444..5484
                     /locus_tag="VF589_11195"
     CDS             4444..5484
                     /locus_tag="VF589_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8258189.1"
                     /translation="MSLFRFLKFPSHDMAIDLGTANTVVYVRGRGVVLNEPSVVAIET
                     INGVKRVKAVGDDAKLMMGKTPDQIEAIRPLRDGVIADIDVAEQMIKHFIQKVHGKRR
                     FPRWPEIVICVPSGSTSVERRAIRDAASNAGASQVWLIEEPMAAAIGADMPVTEPIGS
                     MVVDIGGGTTEVAVLSLRGLAYTTSVRVGGDKMDEAISSYVRRNHNLLIGEATAERIK
                     QQVGIAKPPADGDGLTVHIKGRDLVNGVPKEITINQRQIAEALSEPVGTIIEGVRIAL
                     ENTAPELAADIVDQGIVLTGGGALLQGLDEVLRDETGLPVTVADDPLTCVALGTGRAL
                     EEEIFRGVLTTA"
     gene            5505..6410
                     /gene="mreC"
                     /locus_tag="VF589_11200"
     CDS             5505..6410
                     /gene="mreC"
                     /locus_tag="VF589_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX8258190.1"
                     /translation="MAPPSSRRPGFSRKAQYGLFLGYVAAIGGVVVAVLLLIVAVADP
                     LGFNALRGAALDVTTPISSGGRSVVRFFTGNATDLANYFQAGRQNSELKRQLDGAHRR
                     LVEARAIEFENKRLKRLLKLSSAATDKIAIARIVSSSFDSSRRLATLDVGSAAGIRIG
                     QPVRSPEGLLGRIVETGRYASRVLLITDGANNVPVRLVRDGTPAMATGRGDGTIELKP
                     LEVGENPFKRGDIFITSGVGGIYSPGVPVAMVIRATKDETVARPLADPARVDFAMVQS
                     LYMPAAEAPVEAAPPPPPALPAQQP"
     gene            6407..6922
                     /gene="mreD"
                     /locus_tag="VF589_11205"
     CDS             6407..6922
                     /gene="mreD"
                     /locus_tag="VF589_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX8258191.1"
                     /translation="MSRIALTRGDVRLKTLRRNYVPIGSTVIACSLVILPVVVSSPIV
                     PDFAFLVLIAWRLLRPEMWAATIALPLGFYNDLIAGHPIGQSMALWTLTFLAFDLLDS
                     RVLFRDYWMDWFFASLAILFYISGGWFVAYVMGSTAEFSVMLPQLVASILAFPLVARI
                     VLTLDRWRLTR"
     gene            6919..>8814
                     /gene="mrdA"
                     /locus_tag="VF589_11210"
     CDS             6919..>8814
                     /gene="mrdA"
                     /locus_tag="VF589_11210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8258192.1"
                     /translation="MRRAVPLITENSQAYTFTRRALVLGGLQGGVAALLAGRMAWLSI
                     AENEHYKLLAESNRVQMMLVPPRRGWIVDRAGRPIAVNRSDYRVDLIPDQLKDPERVV
                     AQLAQLLELPADEVERIREELARAAGYQPVPVAENLTFDKYAAVTLRLPEMPGVAPLR
                     GFSRFYPDGAAVGHLVGYVGTANKEEYEAEKNPLLITPGFKIGKDGLEQTMEPRLRGR
                     PGAKRLEVTARGKLVRELSTLSDTSGGTLPLTIDAGLQAFAARRLGEESGSVVVMDCH
                     TGDILCMASMPAYDPNSFSDGIGQSEWKMLAEDERKPLVNKALSSLYPPGSTLKPMAA
                     LALLDAGVEPEETVGCGGGFQLGNRFFRCLGRHGSMNMHRAIAKSCNTYFYSMSRRVG
                     YDTIAVVARKLGLGAKYDLPVVSQSYGTMPDSEWKRRKYKQEWTQSDTLNAAIGQGYV
                     LVNPLQLAVMAARIASGKPVEPRLVNTKKGPIQPFDLPPKHFETVRHGMWEVVNGNGT
                     AGRSKLPLEGIEMGGKTGTAQVRRITGGQRGQSGAWKYRDHGLFVCFAPTANPRYAAS
                     VVIDHGMGGSRAAAPVAKDVLTYLFDKPRALAALATFEEQWGGTLAERMEARARRWAA
                     GQAAAHDA"
BASE COUNT         1358 a         3049 c         3074 g         1333 t
ORIGIN      
        1 gctcgaccgg ccggccgctg gcgcaatggc aggagcagcg cagcggcggt ctcgcgggcg
       61 aggtcgagct cgcgcccctg atcctgctgc cggcgcgcca atgggtctat gcgcgctgcg
      121 acgagcgctt cgagaagatg atgtccgaag agggtcttga ggaagtccgc tcgctgctgg
      181 agcgaaggct ggatccgatg ctgccggtga tgcgggcgat cggggtgcgg gaaatcgccg
      241 gctatctcag cggcgagctg agccgggaga aggcgctggc ggccggccgc accgccaccc
      301 gccaatatgc caagcgccaa tatacctggt tttcccgcca gccgccggcc gactggccgc
      361 gcttcgccga cggggttgcc gcgcaatggc aattgcccta gccgatcccg atgcacgtct
      421 accgcgatga ggatgcggac ctcgcgctga ttcgggggcg gcgggtcgcc gtcgtcggct
      481 acggcaatca gggccgcgcg caggcgctca acctgcgcga cagcgacgtc cccgtcacta
      541 tcgcccttgc cgaggattcc gccagccggg tgcgcgcccg cgaggacggg ttcgacgtca
      601 tgacggcggc cgaggcctcc gcgaaggcgg acctcgtcgt gatgctcgcc gccgacgagc
      661 tgcacacccg cgtctatgcg gaggaggtgg agccgaacct ggcgcagggc gcggccctcg
      721 ttttcgccca cggcctcagc atccgcttcg ggctgatcga gcctcggccg gacctggaca
      781 tcttcctggt cgcgcccaag gggcctggca ccgcgcttcg ccgggaatat gagcagggcc
      841 gcggcctgat cgccctgttc gcggtgcacc aggacaagag cggcacggcc aacgcgaccg
      901 ccctcgccta cgcggcggcg atcggcagcg gccgggtggg gatcctcgag accagcttcg
      961 ccgaggaatg cgaggccgac ctgttcaacg agcaggcggt attgtggggc gcgataccgg
     1021 agctgatcca ggccggcttc gagaccttgg tcgacgccgg cttctccccc gaaatagcct
     1081 atttcgagtg ccttaccgaa gtgaagctga tagccgatct catctacgag cgcggcatag
     1141 ccgggatgcg cgaggcgatc tccaacaccg cggaattcgg ggcgctgaag ggcggcaccc
     1201 ggatcgtcac cgccgagacc cggaccgaaa tgcgccggat cctgcgcgag gtgcgcggcg
     1261 gcggcttcgt caaggatctg gtcgccgatg ccgcggccgg ctacccgctg ctgagggtct
     1321 cgcgggcgca ggcgcgggcg catccgatcg aggcggtggg ggcgcggctg cgggagctcg
     1381 agccgtgatc ttcttcaaaa ctccgcggct ggcgatccgg cgcgcgcgcg aagacgacct
     1441 cgagccgctc ctgctcagct ggtcggatcc cgccatgacc cgctacactg gcatcaggcc
     1501 ggacgtgcgc ggattccttg gccagatgat cgcagacatg caggtcaagc agccgggcga
     1561 gatcgatccc ggcggtccct ggtaccagta tatcgccgag cgcaaggccg acggggcgct
     1621 ggtcggcgat ctcggcgtcg gcttcggcat accgggcgag cggcaggtgg agctgggcta
     1681 ccgtatcctc cccggtttcc accgccaggg ctatgcgcgc gaggcggtgg cggggctgat
     1741 cgattatctg atcgagcgtc acgccatcca ccgcttcgtc ggcatagcgg cggtcccgaa
     1801 catcgcctcg accggcctct tacgctcgct cggcttccgc caggaaggcc gcttccgcca
     1861 gagcttcctc tgccacggcg aatggcttga cgacgattat ctcgccttgc tcgccagcga
     1921 atggcgcggc ggcgcgagcg gctagcgcgg cgggatcgtc cagaggtaga tcgaggcgcg
     1981 ctcggtctcg accgtccgct cgggcttcca attgcccatg tcgtgccaat agctgaccac
     2041 gcgagtgccg ggcttgagct cggcgagcag ccgcggccgc agccggcggt tgaggtcggg
     2101 atagaggaac aaggtgacca cggtggcgtc gccgaaatcg gcctcgaaca gatcctcgtt
     2161 gcggaagctg acgcggccgg tgacgccggc cttgcgcgca ttggcgcggg attcggcgat
     2221 ccgcttcggg tcgatgtcga tgccgacgcc gcgcgcgccg tagcgtaggg cggcggtgat
     2281 cgggatgcgc ccgtcgcccg agccgaggtc gtagacgagg tcgtccttgc cgacccgggc
     2341 cagctcgagc atccgctcca cgaccggctg cggcgtcggc acgtagcgca cgtcgggctt
     2401 gcggagcgcc ttggccgcgc cgggcggccg gtcgccggcc tccgggccca agggtgcgca
     2461 tcccgcgagg gcaacgacaa gcaaagcgac agcagccctc atccccatct cccgctatct
     2521 ccgcccgaac aatttctcga tatcggcgtg gccgagcttc acccaggtcg ggcggccatg
     2581 gttgcactgg ccgctatgcg gcgtcacctc catctcgcgg agcagggcgt tcatctcggc
     2641 gaccgacagc acccggcccg cgcgcacgga gccgtggcag gccatggtcg cggcgacatg
     2701 gtcgagccgc tccttgaggg cgagcgcttc gtcataggcg gcgagctcgt cggcgaggtc
     2761 ggtgaccagg ccgcggacgt cgccatggcc gagcaaggcc ggcgtcgcgc ggaccagcat
     2821 cgcgcggggg ccgaaacgct cgagctcaag ccccatctcg gcaagctcgt ccgcccgcgc
     2881 ctcgagccgg tcgcaggcgg gttcgtcgag ctcaaccacc tccggcagga gcaggctctg
     2941 gcgggcgacg ccgccattcg ccatcgcctt gcgcatccgc tcgagcacca gccgctcgtg
     3001 cgcggcatgc tggtcgacga ggacgaggcc gtcctcggcc tcggcgacga tataggtggc
     3061 ggccacctgt ccgcgcgcca cgccgagcgg gaaagtagca gcgatcgggg gaggctcggc
     3121 cgcgatctcg gcgcgcccgg ccggggcggg ctgataggca tcatgccgct cccagacgct
     3181 cgactggccg aagtcgggac gggcgaagct cggccgctgc gcgggggcgg cggccggccc
     3241 cgcctgccaa ttgccgagcg ccgaggccga agggcgctgg gcgctgcggt ggccggcggc
     3301 ttcgagcgca ttgcggaggc cgccgacgat caggccgcga atgttggccg gatcgcggaa
     3361 ccgcacctcc gtcttggcgg gatggacgtt gacgtcgacc tcctcgcccg gaagctcgat
     3421 gaacaaggcg acgatcgggt ggcgatcgcg ggcgagcagg tcctgatagg cggcgcggac
     3481 ggcgccgatc aacagccggt ccttcaccgg ccggccgttg acgaacagga attggtggtc
     3541 ggcgacgccg cggttgaagg tgggaaggcc cgccaccccg ccgagcgtgg cggggccgcg
     3601 ctggtaatcg accgcgacgc tgttgtcggc cagcccgcgg tcggtgagcc ccgccacccg
     3661 cgccggccgg tcctcgcccg gctgcagcga caggctgcgg cgcccgtcat gcgcgaaggc
     3721 gaagccgatg tcgggacggg ccatggcgag ccgtttcacc acgtcgaggc aggcggcata
     3781 ttcggagcgg gccgagcgca ggaacttgcg gcgcgcgggc accttgccga acaggtcggc
     3841 gacggtgatg cgggtgccgg gcgggagcgc cgccggcccc tccccttcga gccggccgtt
     3901 gtcgatgatc cgcgaccagc cgtcggcggg agcctgtcct gagcctggcg aagggcgcac
     3961 ccggctctcg atggcgagcc gggcgacgct ggcgatcgac ggcagcgcct cgccgcgaaa
     4021 accgagcgtc gcgaccgcct cgatagcgtc gcccgggagc ttcgaagtgg cgtgccgctc
     4081 gagcgccagc gccatgtcgc cgggggccat gccgcagccg tcgtcgacca cttcgatgcg
     4141 gtcgatgccg ccgccggaaa gcgcgattgc gatccgggtc gcgccggcgt cgatcgcgtt
     4201 ttccaccagt tccttcaacg cgctggccgg ccgttcgacc acttcaccgg cggcgattcg
     4261 gttgacgaga tgttcaggaa gacgccttat tgacatgact ctttctctag accaacgggc
     4321 gccatcccgc gagccatgtt aactgtgttc tggacgcatg cggcgggggc accctatagg
     4381 tcgaagacgg gcagtgccgg cccttcgcgg cgcgtcgacg ttgatgaagc gagtttaggc
     4441 gaaatgtctt tgttccggtt cctgaagttt ccctcgcacg acatggccat cgacctcggc
     4501 accgccaaca ccgtcgtcta tgtgcgcggc cgcggcgtcg tcttgaacga gccctcggtg
     4561 gtcgcgatcg agacgatcaa cggcgtcaaa cgcgtcaagg cggtcggcga cgacgccaag
     4621 ttgatgatgg gcaagacccc cgaccagatc gaagcgattc gcccgcttcg cgacggcgtc
     4681 atcgccgaca tcgacgtcgc cgagcagatg atcaagcatt tcatccagaa ggtccacggc
     4741 aagcgccgct tcccgcgctg gccggagatc gtgatctgcg tgccctccgg ttccacctcg
     4801 gtcgagcggc gcgcgatccg cgatgccgcg tccaacgccg gcgcctcgca ggtgtggctg
     4861 atcgaggagc caatggcggc cgcgatcggc gccgacatgc cggtcaccga accgatcgga
     4921 tcgatggtgg tcgacatcgg cggcggcacc accgaggtgg cggtgctctc gctacgcggc
     4981 ctcgcctaca cgacttcggt cagggtcggc ggcgacaaga tggacgaagc catttcgtcc
     5041 tatgtccgcc gcaaccacaa tctgctgatc ggcgaggcca ccgccgagcg catcaagcag
     5101 caggtcggta tcgccaagcc gccggccgac ggcgacggcc tcaccgtcca catcaagggc
     5161 cgcgacctgg tcaacggcgt tcccaaggag atcaccatca accagcgcca gatcgccgag
     5221 gcgctgtcgg agccggtcgg gacgatcatc gagggcgtcc gcatcgcgct cgaaaatacc
     5281 gcgccggaac tcgccgccga catcgtcgac cagggcatcg tcctgaccgg cggcggcgcc
     5341 ctgctccagg ggctggacga ggtgctccgc gacgagaccg gcctccccgt caccgtcgcc
     5401 gacgatccgc tgacctgcgt cgcgctcggc accgggaggg cgctcgagga ggaaatcttc
     5461 cgcggcgtgc tgacgacggc ctaagccgga gcgggtagca gcgaatggcg ccgccatcgt
     5521 cccggcgccc cggattttcc cggaaggcgc aatatggact gttcctcggc tatgtcgcgg
     5581 caatcggcgg cgttgtcgtg gcggtgctgc tgctgatcgt cgccgtcgcc gacccgctcg
     5641 gcttcaacgc gctgcgcggg gccgcgctcg acgtcaccac gccgatcagc tcgggcgggc
     5701 ggagcgtggt ccgcttcttc accggcaacg ccaccgacct cgccaattat ttccaggccg
     5761 gccggcagaa ttcggagctc aagcgccagc tcgacggcgc ccaccgccgg ctggtcgagg
     5821 cacgggcgat cgagttcgag aataagcgcc tgaagcggct gctcaagctg tccagcgccg
     5881 ccaccgacaa gatcgccatc gcccgcatcg tcagctcgtc cttcgattcc agccgccgcc
     5941 tcgccacgct cgacgtcggc agcgccgccg ggatcaggat cgggcagccg gtgcgctcgc
     6001 ccgaaggcct gctcgggcgg atcgtcgaga ccggccgcta tgcctcgcgc gtgctgctga
     6061 tcacagacgg cgcgaacaac gtcccggtcc ggctggtgcg ggacggcacc ccggcgatgg
     6121 cgaccggccg cggcgacggc acgatcgaac tgaaaccgct cgaagtcggc gagaacccgt
     6181 tcaagcgcgg cgacatcttc atcacctcgg gcgtcggcgg catctattcg ccgggcgtgc
     6241 cggtggcgat ggtgatccgc gccaccaagg acgagaccgt cgcgcgcccg ctggccgacc
     6301 ccgcccgggt cgatttcgcg atggtccaga gcctctacat gccggccgcc gaggcgccgg
     6361 tcgaagcggc cccgccgccg ccccctgccc tcccggcgca gcagccatga gccggatcgc
     6421 gctcacccgc ggcgacgtgc ggctgaagac gctgcggcgg aactacgtgc cgatcggctc
     6481 caccgtcatc gcctgctcgc tggtaatcct gccggtggtg gtgagctcgc cgatcgtccc
     6541 cgacttcgcc tttctggtgc tgatcgcgtg gcggctgctc cggccggaaa tgtgggcggc
     6601 cacgatcgcg ctcccgctcg gcttctataa cgacctcatc gccggccacc cgatcggcca
     6661 gtcgatggcc ttgtggacgc tgaccttcct cgccttcgac ctgctcgaca gccgcgtgct
     6721 gttccgcgac tattggatgg actggttctt tgcgtcgctg gccatcctct tctatatttc
     6781 aggcggctgg ttcgttgcct atgtaatggg cagcacggcg gagttttcgg ttatgctgcc
     6841 gcagcttgtg gcgtcgatat tggctttccc cctggtggcg cggatcgtgc tcaccctcga
     6901 ccgctggcgg ctgacgcgat gagacgcgcg gttccgctga tcaccgagaa ttcgcaggcc
     6961 tataccttca cccggcgggc gctggtgctg ggcggcctgc agggcggggt cgcggctttg
     7021 ctcgccggcc gcatggcctg gctgtcgatc gccgaaaacg agcattacaa gctgctcgcc
     7081 gagagcaacc gggtccagat gatgctggtg ccgccgcggc gcggctggat cgtcgaccgc
     7141 gccggacgac cgatcgccgt caaccgcagc gactatcgcg tcgacctcat ccccgaccag
     7201 ctcaaggatc ccgagcgggt ggtcgcccag ctcgcccagt tgctcgagct gcccgccgac
     7261 gaagtggagc gcatccgcga agagctcgcc cgcgccgccg gctaccagcc ggtgccggtg
     7321 gcggagaatc tcaccttcga caaatatgcc gccgtcaccc tgcgcctgcc ggagatgccc
     7381 ggcgtcgcgc cgctcagggg cttcagccgc ttctacccgg acggcgccgc ggtcggccat
     7441 ctggtcggct atgtcggcac cgccaacaag gaggaatatg aggccgagaa gaacccgctg
     7501 ctgatcacgc cgggcttcaa gatcggcaag gacgggctcg agcagaccat ggagccccgg
     7561 ctgcgcgggc ggcccggggc caagcggctc gaggtcaccg cccgcggcaa gctggtgcgc
     7621 gagctttcga ccctgtccga cacgagcggc ggcaccctcc cgctcaccat cgacgccggc
     7681 ttgcaagcgt tcgcggcgcg gcggctgggc gaggaatcgg ggtcggtggt ggtgatggac
     7741 tgccataccg gcgacatcct ctgcatggcc tcgatgccgg cctacgaccc caacagcttc
     7801 tccgacggca tcggccagag cgaatggaag atgctggccg aggacgagcg caagccgctg
     7861 gtcaacaagg cgctgagctc gctctacccg cctgggtcga ccctgaagcc gatggccgcg
     7921 ctggcattgc tcgacgcggg cgtcgagccc gaggagacgg tcggctgcgg cggcggcttc
     7981 cagctcggca atcgcttctt ccgctgcctc ggccgccacg gctcgatgaa catgcaccgg
     8041 gcgatcgcaa agagctgcaa cacctatttc tattcgatga gccgccgggt cggctacgac
     8101 acgatcgcgg tggtggcgcg caagctgggg ctcggcgcca aatacgacct gccggtggtc
     8161 tcgcaaagct acggcacgat gcccgacagc gaatggaagc gccgcaaata caagcaggaa
     8221 tggacccagt ccgatacact taacgcggcg atcggccagg gctatgtgct ggtcaacccg
     8281 ctccagctgg cggtgatggc ggcgcggatc gcgtcgggca agccggtcga gccgcggctg
     8341 gtcaacacca agaaggggcc gatccagccg ttcgaccttc cccccaagca tttcgagacg
     8401 gtgcgccacg gcatgtggga ggtggtgaac ggcaacggca ccgcgggccg cagcaagctg
     8461 ccgctcgagg gtatcgagat gggcggcaag accggcaccg cgcaggtgcg gcggatcacc
     8521 ggcggccagc gcggccagtc gggcgcctgg aaatatcgcg accatggcct gttcgtctgc
     8581 ttcgcgccga ccgccaaccc ccgctatgcc gcctcggtgg tgatcgacca cggcatgggc
     8641 ggctcgcgcg cggccgcgcc ggtggcgaag gacgtgctca cctatctgtt cgacaagccg
     8701 agggccttgg ccgcgctcgc cacgttcgag gagcaatggg gaggcaccct cgccgaacgg
     8761 atggaggcgc gcgccaggcg ctgggccgcg ggccaggccg ccgcccacga cgcc
//
LOCUS       DASVRK010000278        11517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_247219, whole genome shotgun sequence.
ACCESSION   DASVRK010000278 DASVRK010000000
VERSION     DASVRK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247219"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VF589_11215"
     CDS             complement(<1..1107)
                     /locus_tag="VF589_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258193.1"
                     /translation="MPRTARLTKFLSIPLLGWATAAAAGYDLRFAGRDGAELVRYLDA
                     DSINRRGDVVAFWMTDVRGAPGAGGEIVETLGAAHCSELSFRPLQSRRNPTSTLRQPT
                     WVVSTRAAWRQFGEEHRTAPGASIRAVIAMACAATDPAATAEQRLAALTAPPSMPAAP
                     SRVESAAASLAATPSDPATAAPAPAGGLAAASPPSSPAESAALEAAAAAAIASGNIPV
                     GIGTATAGEPLPSARLSAWVDMGASITGQAIEVDAASVDESGASPTGWFRLINPDEEA
                     PTSVSYLLRVDCRKRTINQLGLRRHGAGGTITEERDYGPAGEGPMEVEAETVMEIAYR
                     GLCTGEGARGLRQLAGLPPDPPARSETAAAPPPPP"
     gene            1234..1307
                     /locus_tag="VF589_11220"
     tRNA            1234..1307
                     /locus_tag="VF589_11220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Gln,seq:ctg)
     gene            complement(1403..3337)
                     /locus_tag="VF589_11225"
     CDS             complement(1403..3337)
                     /locus_tag="VF589_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8258194.1"
                     /translation="MLRSVSLTAILLASAAPAFAQRANENAVEAADDAFGTSVGNQQI
                     GLYNVGDVRGFSPITAGNVRIEGLSVTEHGGFTSRIVSGSTIRVGLTAQSYPFPAPTG
                     IADYSLRNSGNEAVLSPVLYLGPNSTVALDIDAQIPIVEDRLSVAAGFTARYDEAFPG
                     EDSRQLQGGAVLRWRPTDRIEVKPFYGRIKVMDDRDIPSIFSGGPYLPPKVERRYFGL
                     DWTQNENERQFYGAVGSAQLADNWQVRAGLFRWENHTKLSLTELYRNVQPDGSAQRTL
                     VAQKDQTAESWSGELRSSYSITEGGRRHTIHLAARGRDTRRAYGGGDVRELGPAVIGR
                     RTDDPEPEFAFGELTRDDVRQITGGVGYELRWPKVGELSLGVQKTDYEKNSRIPGLPT
                     LTTKDSPWLFNGTLALHVTDKLVAYAGYTRGLEESGVAPANAVNRNSAPPALRTSQRD
                     AGIRYAILPDLSLVAGVFDVRKPYFNLDPDRVFRELGTVRHRGIELSLAGRPIEGLSV
                     VAGAVFLDAEVSGEAVDLGIIGPKPVGTTGRLVRGNFDYRLPFLDPVSVDLAINHLAG
                     KVAGAREYAELGGGQLMTEPRTTFDIGARYRFKLGAAPATLRAQITNLFNVYEWDVST
                     SSSFRFSDTRRFLLNLAADL"
     gene            complement(3675..4784)
                     /locus_tag="VF589_11230"
     CDS             complement(3675..4784)
                     /locus_tag="VF589_11230"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX8258195.1"
                     /translation="MILGTAAAAIARPAFSQGRAGGKAGGWYDRAIVIDALAGVRDPY
                     GEDGVLRLSDRAWTETLATGVTLVRDTIFPVGNVADAWGDYQKSIDDKKDELAANPDR
                     LLLVRTAADILKAKRERKLAVLLGTQDTSMIGPELDRMAQLKTDGVMTVQLTYNNRNL
                     CGDGSLEPANAGLSKLGRATIERIEAEKLLLDLSHGGARTMAEATAHSKRPLVISHTA
                     ARALTDHPRNTGDETIRAVAAKGGVVGVYFMPFLTLDSQPKGSDLVRHIEHVANVAGE
                     DHVGIGTDNGVLPIATDEAARKQNREWAMQRMKAGIAAPGEGPDVFPIVADYNSLDRY
                     RRLADALAARGWSTSRLEKLFGGNFLRVYREVWGA"
     gene            4976..7039
                     /locus_tag="VF589_11235"
     CDS             4976..7039
                     /locus_tag="VF589_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX8258196.1"
                     /translation="MRVIGFWVATWLAAMLLAAPAFAQLPQPGKNAIAASLVPESAIV
                     VPGQTVTLALAMRPSKGWHGYWKNPGDSGIETQIAWDLPAGISAGPIQYPVPHRLIIG
                     GLMNYVYEGDYAQLIDVKVPAGLAPGTKLPIRARVDYLACTDEICVPETANVAVDLQA
                     GSAPAAQANRAMFDRFRQALPKPLGSPARFERAGDRFRVAIPLPAGLELADPYFYPLT
                     DGALAYAAPQTVSRAGDMLIVETEAASNGGSLAAVEGVLKLSEGIGLSLQAAPGAVPA
                     AGTPVAQEQAQPALGASAVLAALLGALVGGLLLNIMPCVFPILSLKALSLAKAGGDEA
                     AAKREAVAYTAGAVLICLALGAALLALRAGGAAAGWAFQLQDPRVILSLLLLITAVAL
                     NLAGLFELPALAGGDRLAQRGGTSGAFWTGALAAFVATPCTGPFMGAALGAALVLPTA
                     LALAVFAGLGLGLALPFLALGFVPALRRRLPKPGAWMDRFRRLMSVPMFLTALALAWI
                     LGRQAGVDGMALGLAAATLLALALWWVGRRQGRARAWLPLAPAALAAVAAMLLLPTAA
                     AGPAMASAQGLLKSEPFSETRLAALQQEKRPVFVYFTADWCVTCKVNEKGALERDQVA
                     QAFDDKQVAVLVGDWTRGDAAIGRFLEKHGRSGVPLYLYYEPGKPVEILPQVLTPSRL
                     VSMVS"
     gene            7055..7672
                     /locus_tag="VF589_11240"
     CDS             7055..7672
                     /locus_tag="VF589_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX8258197.1"
                     /translation="MKPMLTAFTAFTALAIVAAPAAAAPTVGQPAPNFKLADANGKPV
                     SLSDFKGKTVVLEWNNPGCPFVQKHYGSGNMQKAQAAAVKEGVVWLSINSSAPGKQGH
                     MNAAEAKAFVAKAGARPTAYLLDPRGVVGKVYEAKTTPHMYIVNKAGTLVYAGGIDDK
                     PTPNPADIDGARNHVLAALSELNAGKSVSVATSRPYGCAVKYSDS"
     gene            7872..8519
                     /locus_tag="VF589_11245"
     CDS             7872..8519
                     /locus_tag="VF589_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8258198.1"
                     /translation="MKAWPPARLAARLATASALSLALAACGPDIAETLADLPVGQAAP
                     DFRASEANGRPVTLADFAGRTIVLEWNNPDCPTVRKHYDSGNMQNTQAAATAQGAVWL
                     TINSSAEGNQGHMTPAEAKAFTATHPSRRTAYLLDPNGEVGRAYGAKATPHLYVINPA
                     GTLVYNGGIDDKPTQDPADIPGARNHVLAALGELKAGKPVSVPTSRAYGCSVKYG"
     gene            8538..9191
                     /locus_tag="VF589_11250"
     CDS             8538..9191
                     /locus_tag="VF589_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8258199.1"
                     /translation="MADTVQLHGWRYSAYTRIARLALLSKGVGHETIEVDPFGKLPDA
                     YLRLHPFGRVPVLTHEAFTLFETSAITRYVDRAFEGRPLQPKGAAALARMDQVISVVD
                     SYAYWPMVRQVASHAFFRPFFGDLSSREEIDAGLEASRKVLSFLDGVAAEGAVLSGRD
                     FTLADCHLAPMMDYFVRAEEGRAALSTHPALQEWWDRLSVQDVLKATDPFPAEPASR"
     gene            complement(9229..10011)
                     /locus_tag="VF589_11255"
     CDS             complement(9229..10011)
                     /locus_tag="VF589_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX8258200.1"
                     /translation="MSNPPAATRKAVIHRMVMPNHICPYGLKAVHLLKRAGYEVEDHH
                     LTTREETDAFKAEHKVATTPQVFIGGERIGGHDDLRRFLGKRVADPKATSYRPVIALF
                     SVTALTALAASYAVTGSAFTLLAAEWFIGFSMVVLALLKLQNIESFATMFLNYDLLAK
                     RWVPYSYIYPFAEGLAGVLMISGALTWLSVPIALFIATIGAVSVVKAVYIDKRELKCA
                     CVGGSSNVPLGFISLTENLMMIAMAVWMALAALGLASGGIHM"
     gene            10068..10472
                     /locus_tag="VF589_11260"
     CDS             10068..10472
                     /locus_tag="VF589_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8258201.1"
                     /translation="MVQSQGPKSLTIGQLAAGGNVGVETIRFYQRKGLLETPTRESGI
                     RRYGSEHLRRLRFIKQAQAAGFTLEEIRELLELDAGEDRTRARELARSRVEALDAKIA
                     ELNRARDALQRLATECGAGGKGPCPILASFDV"
     gene            complement(10522..>11517)
                     /locus_tag="VF589_11265"
     CDS             complement(10522..>11517)
                     /locus_tag="VF589_11265"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX8258202.1"
                     /translation="TPVEAGELRETWNDRVSQGPPVGATREFEGWSMGTAMPLPRSEH
                     AVAEFGGKIWVLGGYPPGRLPSNLVQVYDPATGKWSLGPPLPEAIHHTHVASVGGKLY
                     MLGGEVEGASTGRPERFVSNVWMHDPAVGGWVARAPMPTPRGGGGKAVIDGKIYVAGG
                     RPPGGSAFEVYDPATDRWEKLPDLPSQRNHLAMVAIGGKIYVAGGRTGPGAMAERVDT
                     VEIYDPKTRRWSKGTPLPAPRGGITGAAHAGCLFVFGGEGEKTHVLGLTPNSYGYDPR
                     ADRWTKLPDLPIAVHGLKGSAVIGGRIFLPGGGITLGGNSGTNAMQVYRPTMKCE"
BASE COUNT         1750 a         4015 c         3851 g         1901 t
ORIGIN      
        1 gggcggcggc ggcggggcgg cggcggtttc ggagcgcgcc ggcggatcgg gcggcagccc
       61 ggcgagctgg cgaaggcccc tcgcgccctc gccggtgcac aggccgcgat aggcgatctc
      121 catcaccgtc tccgcttcga cctccatcgg cccctcgccg gccgggccgt aatcgcgctc
      181 ctcggtgatc gtgccgcccg ccccgtgccg gcgcaggccg agctggttga tggtgcgctt
      241 gcggcagtcg acccgcagca aataggagac cgaggtcggt gcctcctcgt cggggttgat
      301 cagccggaac caaccggtcg gcgaggcgcc gctttcatcc acgctggcgg cgtcgacctc
      361 gatcgcctgg ccggtgatcg aggcgcccat gtcgacccag gcgctgagcc gggccgaggg
      421 cagcggctcg ccggcggtgg cggtgccgat gccgaccgga atattgccgg acgcgatcgc
      481 cgccgccgcg gccgcttcga gcgcggccga ttcggcgggc gaagagggcg gggacgcagc
      541 cgcgagcccg ccggcgggcg cgggcgccgc cgtggcgggg tctgacggcg tcgcggcgag
      601 cgaggctgcg gcgctctcga cccggctcgg cgcggccggc atggagggcg gggcggtgag
      661 ggcggcgagg cgctgttcgg cggtcgcggc cgggtcggtc gcggcgcagg ccatggcgat
      721 caccgcccgg atcgaggcgc ccggcgcggt gcgatgctcc tcgccgaact gccgccacgc
      781 cgcgcgggtc gacaccaccc aggtcggctg gcgcaaggtg ctggtggggt tgcggcgcga
      841 ctggagcggc cggaacgaaa gctcggagca atgggcggcg ccgagcgtct cgacgatctc
      901 gccgccggcg ccgggcgcgc cgcgaacgtc ggtcatccag aaggcgacga cgtcgccgcg
      961 gcggttgatg ctgtcggcgt cgagatagcg gacgagctcg gcgccgtcgc ggccggcaaa
     1021 gcgcaggtcg taccccgcgg ccgccgccgt cgcccagcca agtagcggga tcgaaaggaa
     1081 ttttgtcagg cgggcggtgc gcggcatagg cctgcgtctc taccgtgcaa gccccccgtc
     1141 atgcaactgt cggcgggcgc cgcgagcgct tcgccggccg cggcccgccc cttgacccgc
     1201 cccggcgtgc ggcctatttc gcccgcctca ccttgcccga tcgtctaatg gtaagactac
     1261 ggactctgac tccgtcaatc gaggttcgaa tcctcgtcgg gcatccagtt caccgcttca
     1321 tcccagcctc cgccgcctcc cggccgtggc tccacgtccg tgggattgag ccgcctcaca
     1381 tatcattccg agcccgggtc cttcaaagat ccgctgccag gttgagcagg aaccgccggg
     1441 tgtcgctgaa gcgaaacgat gagctggtgg agacgtccca ttcataaacg ttgaagaggt
     1501 tcgtgatttg ggccctcagc gtggccggcg cggcgcccag cttgaagcgg tagcgggcgc
     1561 cgatgtcgaa ggtcgtccgc ggctcggtca tcagctgccc gccgccgagc tccgcatatt
     1621 cacgggcgcc ggcgaccttg cccgccaaat ggttgatggc gaggtccacc gaaaccggat
     1681 cgaggaaggg caggcggtaa tcgaaattgc cgcgaacgag ccttcccgtg gtgccgaccg
     1741 gtttcgggcc gatgatgcca aggtccaccg cttcgccgga gacttcggcg tcgaggaaga
     1801 ccgcgccggc gacgacgctc agcccctcga tcggccgccc tgccagcgac aattcgatgc
     1861 cgcgatgccg caccgtgccg agttcgcgaa agacccggtc cggatcgagg ttgaaatagg
     1921 gtttgcgcac gtcgaagacg cccgcgacca ggctgaggtc gggcaggatc gcgtagcgaa
     1981 tccccgcgtc gcgctggctg gtcctcagcg ccggcggcgc cgaattccgg ttcaccgcat
     2041 tggccggagc gaccccgctc tcttccaggc cgcgcgtgta gcccgcatag gcgaccagct
     2101 tgtcggtaac gtggagcgcc agagttccgt tgaagagcca cgggctgtct ttggtcgtta
     2161 gcgtcggaag tccgggaatc cggctattct tttcatagtc ggttttctgc acccccaggc
     2221 tgagttcgcc caccttcggc cagcgcaatt catagccgac cccgccggtg atctggcgca
     2281 cgtcgtcgcg cgtgagctcc ccgaaagcga attccggctc cgggtcgtcg gtgcgccgtc
     2341 cgatcaccgc cggcccaagc tcccgcacgt cgccgccgcc ataggctcgc cgcgtgtcgc
     2401 ggccgcgggc ggcgagatga atcgtgtgac gccggcctcc ctccgtgatc gagtaggaag
     2461 agcgcaattc tcccgaccag gactccgccg tctggtcctt ctgcgccacc agcgtccgct
     2521 gcgccgagcc atccggctga acgttgcgat agagttcggt gaggctgagt ttcgtatgat
     2581 tctcccagcg gaagagaccg gccctgacct gccagttatc ggcaagctgg gcagagccca
     2641 cggcgccata gaattgccgc tcattctcgt tctgggtcca gtcgagaccg aaatatcggc
     2701 gctctacctt cggaggcaga tagggcccgc cactgaagat ggacgggatg tcccggtcgt
     2761 ccatcacctt gatgcgcccg taaaagggct tgacctcgat ccggtccgtg ggccgccacc
     2821 gcagcacggc tccgccctgc aattgccggc tatcctcgcc agggaaagcc tcgtcgtagc
     2881 gggcggtgaa accggccgcc acgctgagcc ggtcctcgac gatggggatc tgggcgtcga
     2941 tatccagcgc cacagtgctg ttggggccga gatagagcac cgggctcaag acggcttcgt
     3001 tgccggaatt cctcagcgaa taatcggcaa tgccggtcgg ggccggaaag gggtagcttt
     3061 gcgcggtcag gcccacccga atcgtcgaac cgctcacgat gcggctggtg aagccgccat
     3121 gttcggtgac cgaaagtccc tcgatccgga cgttccccgc ggtgatgggg ctgaagcccc
     3181 ggacgtcccc gacattgtag aggccgatct gctggttgcc gacgctcgtg ccgaaggcgt
     3241 cgtcggccgc ctcgaccgca ttctcattgg cgcgctgggc gaaagcgggc gccgcggagg
     3301 cgagcaatat ggcggtaagg ctgacggacc ggagcatgtg tgttccttca ctttggtctg
     3361 tcctttgtgc gtctcctctc cgatccggat aagtgccgga ggtcgcaccc ccgggtcgtg
     3421 acctatggca tgtggagggc tgctcatgcc gtgcggcatt cccccgtcgg aaggccgtcc
     3481 gtcatcccag cgaaagctgg gatctccctt ctttcccgag ccgcaagatt cagctttcgc
     3541 tggaatgacg ggaggcttgc gctggaatga cgaggggcgg tgcgggtcgt gcgggtgatg
     3601 cagagcccgg ccatccccgc taccggccgc gccgccttgc gcctcactcg atggcggcgc
     3661 ctggggtgga gcggtcaggc gccccagact tcgcggtaga cccggaggaa atttcccccg
     3721 aacagctttt ccaggcgcga agtggaccag ccgcgcgcgg caagcgcgtc ggcgagccgg
     3781 cgatagcggt cgaggctgtt atagtcggcg acgatcggga aaacgtcggg gccttcgccg
     3841 ggcgcggcga tgccggcctt catccgctgc atcgcccatt cccggttctg cttgcgagcc
     3901 gcctcgtcgg tggcgatcgg cagcacgcca ttgtcggtgc cgatgccgac atggtcctcg
     3961 cccgccacgt tcgcgacatg ctcgatgtgg cggacgaggt cgcttccctt gggctggctg
     4021 tcgagggtca ggaacggcat gaaatagacg ccgacgaccc cgcccttggc ggcgaccgcg
     4081 cggatggttt cgtcgccggt gttgcgcggg tgatcggtga gcgcgcgcgc ggcggtgtgg
     4141 ctgatcacca gcgggcgttt ggaatgcgcc gtcgcctccg ccatcgtccg cgcgccgcca
     4201 tggcttaggt cgagcagcag tttctccgcc tcgatgcgct cgatcgtcgc ccggcccagc
     4261 ttcgagaggc cggcattggc cggctcgagc gagccgtcgc cgcacaaatt gcggttattg
     4321 taggtcagct ggacggtcat cacgccgtcc gtcttcagct gggccatacg gtcgagctcc
     4381 ggcccgatca tcgacgtatc ctgcgtgccg agcaggaccg ccagttttcg ttcacgcttg
     4441 gccttcagga tatccgcggc ggtgcgcacc agcagcaggc ggtcggggtt ggcagcgagc
     4501 tcgtctttct tgtcgtcgat gctcttctgg taatcgcccc aagcgtcggc gacgttgccg
     4561 accgggaata tggtgtcgcg caccagggtg acgccggtgg ccagcgtttc ggtccatgcc
     4621 cggtcgctca gccggagcac gccgtcctcg ccataaggat cgcggacccc ggcaagcgcg
     4681 tcgatgacga tggcacggtc ataccatccc cccgctttgc cccccgctct cccttgagag
     4741 aatgcaggcc gcgcgatggc cgcagccgcg gttcccagga tcatagtccg tcggctcacg
     4801 cgcatgtcgc acctcccctt tctagtaagc ccccgtacat cgccagtcct cgatccggcc
     4861 agtgccggag gtagtgcccg ccgaccatga ccttcggcat atggtaaggc ggctcgagct
     4921 gtgggagaat cgcccccatg gcaggtcatt tcagatgaat cgccaaggag gttccgtgcg
     4981 ggttattggt ttctgggtcg cgacctggct ggcggcgatg ctgctcgccg cgcccgcttt
     5041 cgcccagctt ccgcaaccgg gcaagaatgc catcgcggcg agcctcgttc cggaatccgc
     5101 aatcgtcgtg cctggccaga cggtgacgct cgcgctggcg atgcggccgt ccaagggctg
     5161 gcacggctat tggaagaatc ccggcgacag cggcatcgag acccagatcg cctgggattt
     5221 gccggccggc atcagcgcgg ggccgatcca atatcccgtt ccccaccggc tgatcatcgg
     5281 cggcctgatg aactacgtct atgagggcga ttacgcgcag ctcatcgacg tgaaggtgcc
     5341 cgccggcctc gcgcccggca ccaaattgcc gatccgcgcc cgggtcgatt atctcgcctg
     5401 caccgacgag atctgcgtgc ccgagacggc caacgtcgcc gtcgatctcc aggctggatc
     5461 ggcgccggcc gcgcaggcca accgggcgat gttcgaccgc ttccgccagg cgctgcccaa
     5521 gccgctcggc agccccgccc ggttcgagcg cgccggcgac cgcttccgcg tcgccatccc
     5581 gcttccggcc gggctcgagc ttgccgatcc ctatttctat ccgctgaccg acggcgcgct
     5641 cgcctatgcg gcgccgcaga ccgtctcgcg ggcgggcgac atgctgatcg tcgagaccga
     5701 ggcggcttcg aacggcggca gcctcgcggc ggtcgagggc gtgctgaagc tcagcgaggg
     5761 catcggcctg tcgctgcagg cggcgccggg cgccgttccc gccgccggaa cccccgtcgc
     5821 acaggagcaa gcgcagccgg cgctcggcgc ctcggccgtg ctcgccgcct tgctgggcgc
     5881 gctcgtcggc gggctgctgc tcaacatcat gccctgcgtc ttcccgatct tgagcctgaa
     5941 agcgctgagt ctcgccaagg cgggcggcga cgaagccgcg gccaagcgcg aagccgtcgc
     6001 ttatacggcc ggcgccgtat tgatctgcct cgcgctcggc gccgccttgc tggcgctgag
     6061 ggcagggggc gccgccgccg gctgggcgtt ccagctccag gacccgcgcg tcatcctgtc
     6121 gctgctcctg ctcatcaccg ccgtcgcctt gaacctcgcc ggcctgttcg agcttcccgc
     6181 tctggccggc ggcgaccgct tggcgcagcg gggcgggacc agcggcgcct tctggaccgg
     6241 cgcgctcgcc gccttcgtgg cgacgccctg caccggcccc ttcatgggcg ccgcgctcgg
     6301 ggccgccctc gtgcttccca ccgcgctcgc gctcgccgtc ttcgccgggc tgggcctggg
     6361 cctcgccttg ccgttcctcg ctctcggctt cgtgcccgcg ctccgccgcc gcctgccgaa
     6421 gccgggcgcg tggatggacc ggttccggcg gctgatgtcg gtgccgatgt tcctgaccgc
     6481 gctcgctttg gcctggatcc tcggccgcca ggccggcgtc gacggaatgg cgctcgggct
     6541 ggccgcggcg acgctgctcg cgctcgcttt gtggtgggtc ggacgacggc agggccgtgc
     6601 gcgcgcatgg ctgccgctgg cgcccgccgc gctcgccgcc gtcgcggcca tgctcctcct
     6661 tcccaccgcc gccgccgggc cggccatggc gtcggctcaa ggcctgctca agtcggagcc
     6721 gttcagcgag acgcggcttg ccgccctgca acaggaaaag cggcccgtct tcgtctactt
     6781 caccgccgat tggtgcgtca cctgcaaggt caacgagaaa ggcgcgctcg agcgcgacca
     6841 ggtcgcccag gctttcgacg acaagcaggt ggcggtgctg gtcggcgact ggacccgcgg
     6901 cgatgccgcc atcggccgct tcctcgaaaa acacggccgc tcgggcgtgc cgctctacct
     6961 ctattacgaa ccgggcaagc cggtcgaaat cctgccgcag gttctgacgc cctcgcggct
     7021 cgtgtcgatg gtcagctgaa aaaggagtaa caagatgaag ccgatgctca ccgctttcac
     7081 cgcttttacc gcgctcgcaa tcgtcgccgc ccccgccgcc gcggccccga cggtcggcca
     7141 gcccgcgccc aacttcaagc ttgccgacgc caacggcaag ccggtcagcc tctccgactt
     7201 caagggcaag acggtggtgc ttgaatggaa caaccccggc tgccccttcg tgcagaagca
     7261 ttatggcagc ggcaacatgc aaaaggcgca ggccgccgcc gtcaaggagg gcgtggtctg
     7321 gctcagcatc aattccagcg cgccgggcaa gcagggccat atgaacgccg ccgaagccaa
     7381 ggccttcgtc gccaaggcgg gcgcccgccc gaccgcctat ctgctcgatc cgcgcggcgt
     7441 cgtcggcaag gtctacgagg ccaagacgac gccccacatg tacatcgtca acaaggccgg
     7501 cactttggtc tatgccggcg gcatcgacga caagccgacc cccaacccgg ccgacataga
     7561 cggcgcccgc aaccacgtcc tggccgcgct gtcggagctc aacgccggca agtccgtctc
     7621 ggtcgccacc agccgcccct atggctgcgc cgtcaaatat tcagacagct gagcttgcgt
     7681 aaatcctccc ccggttttcc gggggagggg gaccaggcga agcctggtgg aggggcttct
     7741 tctgactcgg aagagcccct ccaccaccgc ctttggcggc ggtccccctc cccgcgaaat
     7801 cgcggggagg aatttagagg ccgacatcct tccgcagcta cgaacatctc gcatcgctag
     7861 aggagcatga gatgaaagca tggccacccg cccgcctcgc cgcccgcctc gccaccgcgt
     7921 cagccctgtc gctagcgctt gccgcttgcg gccctgacat cgccgaaacc ctcgccgatc
     7981 tcccggtcgg gcaggccgcg cctgatttcc gcgccagcga ggccaacggc cgccccgtca
     8041 ccctcgcgga tttcgccggc aggacgatcg tactcgaatg gaacaacccc gactgcccca
     8101 ccgtcaggaa gcattacgac agcggcaaca tgcagaacac ccaggccgcc gccaccgccc
     8161 aaggcgcggt ctggctgacg atcaacagca gcgccgaagg caatcagggc cacatgaccc
     8221 ccgccgaagc caaggccttc accgccaccc acccaagccg ccgcaccgcc tacctcctcg
     8281 accccaatgg ggaggtcggc agggcctacg gcgccaaggc caccccgcat ttatacgtca
     8341 tcaaccccgc cggcaccctg gtctacaatg gcggcatcga cgacaaaccg acccaggacc
     8401 cggccgacat cccgggcgcc cgcaaccatg tgctggccgc cctgggcgag ctgaaggcgg
     8461 gcaagcctgt ttcggtgccg acaagccgcg cttatgggtg ctcggtgaaa tacggttgag
     8521 cggccaggga ggctcggatg gcggacacgg tccagcttca tggatggcga tacagcgcct
     8581 acacccggat cgcccggctg gcgctgctgt cgaagggggt tgggcacgag acgatcgagg
     8641 tcgatccgtt cggcaagctt cccgacgcct atctgaggct gcatccgttc gggcgggtgc
     8701 cggtgctgac ccatgaggcg ttcacgcttt tcgagacgag cgcgatcacg cgctatgtcg
     8761 accgggcgtt tgaggggcgg ccgttgcagc cgaagggcgc cgcggccctg gccaggatgg
     8821 accaggtgat ctcggtcgtc gactcctacg cctattggcc gatggtcagg caggtggctt
     8881 cccatgcctt cttccgcccg ttcttcggag acctgtccag ccgcgaggag atcgacgccg
     8941 gcctggaagc ctcgcgcaaa gtgctgtcgt ttctggacgg agtcgcggcc gagggcgcgg
     9001 tgctgagcgg gcgcgatttc acccttgccg actgtcacct cgccccgatg atggattatt
     9061 tcgttcgcgc cgaggaggga agggcagccc tttccaccca ccccgctttg caggaatggt
     9121 gggaccggct gtccgtccag gacgtcctca aggcaaccga cccgttcccg gcagagccgg
     9181 cctcccggtg aaaccgggag gtggcttcgg cctcatgggc gggctgcgtc acatgtggat
     9241 tcctccactc gcaaggccga gtgccgcgag agccatccag acggccatgg cgatcatcat
     9301 caaattctcg gtcagcgaga tgaaaccgag cggcacattg ctcgaaccgc cgacgcaggc
     9361 gcatttcagc tcccttttgt cgatgtagac cgccttgacc accgacaccg cgccgatcgt
     9421 cgcgatgaac agcgcaatcg gaaccgacag ccaggtgagc gcccccgaga tcatcagcac
     9481 gcccgcgagc ccctccgcga acggatagat gtagctgtac ggcacccagc gcttggcgag
     9541 caggtcgtag ttcaggaaca tcgtcgcgaa gctctcgatg ttctgcagct tcaaaagcgc
     9601 cagcacgacc atcgagaagc cgatgaacca ttcggccgcg agcagggtga atgcactgcc
     9661 cgttaccgcg tagctcgccg ccagcgccgt cagcgcggtc accgagaaaa gggcgattac
     9721 cggccggtag ctggtcgcct tcgggtcggc gacacgcttg ccgagaaagc gccgaagatc
     9781 gtcatggccc ccgatgcgtt cgccgccgat aaaaacctgc ggcgtcgtgg cgaccttatg
     9841 ctcggccttg aacgcgtccg tctcctcgcg cgtggtgagg tgatggtctt cgacctcata
     9901 gccggcgcgc ttcagcaggt gcaccgcctt cagcccgtaa gggcaaatgt ggttcggcat
     9961 caccatgcgg tggataaccg ccttcctggt ggccgccgga gggttgctca tgtccgacat
    10021 ttcacgactc cttgtgatct tgtcggacct gatataggtt acgtaccatg gtacagagtc
    10081 aaggccctaa atcactgacg atcgggcaac ttgccgccgg cggcaacgtg ggcgtcgaga
    10141 cgatccgctt ctaccagcgc aagggcctgc tcgagacgcc aacgcgcgag agcgggatca
    10201 ggcgctacgg ctccgaacat ctccgccgat tgcgtttcat caagcaggcg caggccgccg
    10261 gcttcacgct cgaggagata cgggagcttc tggagctgga cgcgggcgag gatcgcaccc
    10321 gcgcccgcga gctggcgagg agccgcgtcg aagctctgga tgcgaagatc gccgaactca
    10381 accgcgcccg cgacgcgctc cagaggctgg cgacggaatg cggcgcaggc ggcaagggcc
    10441 cttgccccat cctcgcttcg ttcgacgtct gagccatcgc atgggttatc cgaactagcc
    10501 ttcaaccgcg aggccagtcc ctcactcgca cttcatcgtc ggccggtaga cctgcatagc
    10561 gttggtgccg ctgttcccgc cgagcgtaat gccgccgccc ggcaggaaga ttcggccgcc
    10621 gatcaccgcg ctgcctttga ggccgtggac ggcgatgggg aggtccggga gcttggtcca
    10681 gcggtcggcg cgggggtcgt agccgtagct gttgggggtg aggccgagca cgtgggtctt
    10741 ctcgccctcg ccgccgaaga cgaacaggca gccggcgtgg gccgcgccgg tgatgccgcc
    10801 gcgcggtgcc gggagcggcg ttcccttgct ccagcggcgg gtcttgggat cgtagatctc
    10861 gaccgtgtcg acccgctcgg ccatcgcgcc ggggccggtg cggccgccgg cgacgtagat
    10921 cttgccgccg atcgcgacca tggcgaggtg gttgcgctgg ctcggcaggt cgggaagctt
    10981 ctcccatctg tcggtcgccg ggtcgtagac ctcgaaggcg gagccgccgg gcgggcggcc
    11041 gccggccacg tagatcttgc cgtcgatcac ggccttgccg ccgccgccgc gcggcgtcgg
    11101 catcggcgcg cgcgccaccc agccgccgac cgccggatcg tgcatccaca cattggacac
    11161 gaaccgctcc ggcctgcccg tgctggcgcc ttcgacctcg ccgccgagca tgtagagctt
    11221 gccgccgacc gacgcgacat gcgtgtggtg gatcgcctcc ggcagcggcg gcccgagcga
    11281 ccatttgccg gtggcgggat cgtagacctg gacgaggttg gaagggagcc gcccgggcgg
    11341 atagccgccg agcacccaga tcttgcctcc gaattccgcg accgcatgtt cggagcgcgg
    11401 caggggcatg gccgtgccca tgctccaccc ctcgaactcg cgcgtcgctc ccacgggtgg
    11461 gccctggctc acgcggtcgt tccacgtctc gcgcagctcg ccggcctcga ccggcgt
//
LOCUS       DASVRK010000279         6251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_158989, whole genome shotgun sequence.
ACCESSION   DASVRK010000279 DASVRK010000000
VERSION     DASVRK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158989"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1756)
                     /locus_tag="VF589_11270"
     CDS             complement(251..1756)
                     /locus_tag="VF589_11270"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX8258203.1"
                     /translation="MSDGNEVELKLEIDPADAARLIDHPLVKRVEPRRARQVSTYYDS
                     GKGALRAAGYSLRLRRTGDRVVQTVKRQEDGAAGLFDRPEWEREIAGDQIDFDALAET
                     PVGPLVAKLRKRLKPIVSAEVERTIWLLSHDGAEIEMILDRGPIRGGKREAELSEIEL
                     ELKRGKPAALIDAARSLAKDVPLKIGVMTKAERGFALANGGLDKAAKAGRVRVSPEMN
                     VAAGLTAIVHACLRHFRLNEPLVVARRDPAALHQARVAMRRLRSAFSLFASAAGDEEF
                     GRLREELRWFTGQLGDARNLDVFLKRLPYGRDTKTFRKRLEAARRDAYDRVLEALASK
                     RMRLMMLDLVAWVELGRWRSGKAAAAPLTDFAEAQLDRRWRKVKKRGRDIAGIDPEAR
                     HRLRIDIKKLRYAVEFMAGLQAEEGRNKAFAGGLEAMQESLGELNDMETARELIAGLP
                     KEVRAAAPASAFAALDPKEQAARTEALIADAQAGHARLKEAGPFWRSAAAG"
     gene            1904..2602
                     /locus_tag="VF589_11275"
     CDS             1904..2602
                     /locus_tag="VF589_11275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8258204.1"
                     /translation="MFMTRAILFDVDGTLVDSNDLHAAAWREAFRHFEVELSHETVRG
                     QIGKGGDNLIPALLPPDLVESRQKEIEDFRSDLFKRDYLPRAVPFPGVRALFERLYAD
                     GVKIVLASSAKSEELQFHLNLIGASDFISATTSQDDVESSKPDPDIFAAALAKVAPIA
                     AEDALVVGDTPYDVIAAARAGLRTIALRSGGFPDEALLGAGAAGLYNDVEDLLARYDE
                     WLAAAGQNSLAMPA"
     gene            complement(2575..3375)
                     /locus_tag="VF589_11280"
     CDS             complement(2575..3375)
                     /locus_tag="VF589_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX8258205.1"
                     /translation="MRMFPISAAVAAASALLPAEALRAQDERGFCPDRPGLGTPACTL
                     DRGRFALELGVIDWTVDREDGARADLVAGGDLLLRYGLTGSLEVQAGWTLFGHSRLRS
                     GGAVETTSGVGDVLIALRQNLQNPDGSGLAVAIMTYATLPTGGAALGAGDWGAGVIVP
                     LSYELPRGFALGFTATVEAAVDGDRDGRHLAYGGIAGLDLPLSDSLGATVEVAARRDR
                     DPEATEWLAGVSAGWSPRADLQLDAGANIGLSRAAPDLQLYAGIAKLF"
     gene            3464..4093
                     /locus_tag="VF589_11285"
     CDS             3464..4093
                     /locus_tag="VF589_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8258206.1"
                     /translation="MAKKKKAVVAAAEAVQEVDAGAAEAVAPYRKALPVRALSWVSEV
                     GDQPPLRALCGAMILAGLAGGGPRLARAGGRALLAHSLATGAKNFVKHRVDRTRPRQV
                     VESAGIADHKMKPGRNEAKAETSFPSGHSAGAIAVARGFAREFPEYQAPALAAAGLVA
                     LAQIPRCAHYPTDVGAGLAIGLAAEAATDAAWNAAASLVEERLEDANSR"
     gene            complement(4111..5658)
                     /locus_tag="VF589_11290"
     CDS             complement(4111..5658)
                     /locus_tag="VF589_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8258207.1"
                     /translation="MTDPRSYTPRAIWAGYLAMIVGNFMAILDIQIVASSLREIQAGV
                     SASADEIAWVQTSYLIAEVIAIPLSGLLGRALSMRTLFTFSALGFTLMSLACATAWSI
                     ESLILFRTLQGFLGGAMIPTTMAALFILFPEAKRAPAIVLVGMVSTLAPSVGPSLGGW
                     ITDSMGWQWLFLINLGPGLAAAALVWRFSPLRDAEPALLRRIDIWGLVGMAVFLGSLE
                     IVLEEGPKHDWLQDDTVFMMAVAMILGGAIFFWRAFTAAAPIVDLRVFANRNFIVGSL
                     VSMIAGVGLYGSVYLMPLFLGGVRGYSSTQIGEVMVVTGLAMFLTAPIVGKLQAKVDL
                     RFLLGLGILLTAFGMWQNAQLTAESGFWELALPQALRGCGLMMCMIPMTGIALGTLPA
                     DRVQNASGLFNLTRNLGGALGLAVINTLFREGQDLHRTELAAAVSAGHADVPGWLDQM
                     TQRMAASGVADPARAALAQLAGMVEREATVMAFNNIFVSLALVFALLLPLVLFTRQAK
                     GAAPAGH"
     gene            complement(5709..>6251)
                     /locus_tag="VF589_11295"
     CDS             complement(5709..>6251)
                     /locus_tag="VF589_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX8258208.1"
                     /translation="AAVSAQRGQAGALVAATAGAEASIAAGQAQVQAAEANLRQLKLD
                     LARTVIRAPVAGVVGNRSVREGQLVRPGQQLLAIVPVAQAYVVANFKETQLGKMRVGQ
                     PVELEVDAYPGLKVRGRIESLAPASGAQFALIPTDSATGNFTKIVQRVPVRIVVEKDA
                     LAAQLLRPGLSVEATVDTRG"
BASE COUNT         1011 a         2191 c         2161 g          888 t
ORIGIN      
        1 tgtagctgga aaatgaaggc gctcacctag gcgggcgggg cggccaagtc aagttcgtcg
       61 gccactccgc gccatgcccc tgccggggcg cgccgattcg gtccgccgaa gctccgccac
      121 cgctcgtcgc ccgggcgtga acccgccgcc gcatccggcg ttcaagcccc agtcccgaga
      181 atgcaagtcc cgagaatgca aatcccccct gaagcgcctc ggccgcaagc acggccggag
      241 cgctttggcg tcaccccgct gccgccgagc gccagaacgg ccccgcctcc ttcagccgcg
      301 catgccccgc ctgcgcgtcg gcgatcaaag cctcggtccg cgccgcctgc tccttggggt
      361 cgagcgcggc gaaggccgat gccggcgccg cggcgcgcac ttccttcggg agccccgcga
      421 tgagctcgcg cgccgtctcc atgtcgttga gctcgccgag gctctcctgc attgcctcca
      481 gaccaccggc aaaggccttg ttccgcccct cctcggcctg gaggccggcc atgaactcga
      541 cggcgtaacg caatttcttg atgtcgatcc gcagccggtg gcgcgcctcc gggtcgatgc
      601 ccgctatgtc gcggcccctt ttcttgacct tgcgccagcg ccggtcgagc tgcgcctcgg
      661 cgaagtccgt cagcggagcg gcggcggcct tgccgctccg ccaccggccg agctcgaccc
      721 aggcgacgag gtcgagcatc atcagccgca ttcgcttcga tgccagcgct tcgagcacgc
      781 gatcataagc gtcgcggcgc gccgcctcga gccgcttacg gaaggtcttg gtgtcgcggc
      841 cataaggcag ccgcttcagg aacacatcga ggttgcgcgc gtcgccgagt tggccggtga
      901 accagcgcaa ttcctcgcgc agccgcccga attcctcgtc cccggccgcc gaggcgaaca
      961 aagagaaagc cgaacgaagc cgccgcatcg ccacccgcgc ctggtggagc gcggccggat
     1021 cgcggcgggc gaccacgagc ggctcgttga gccggaaatg gcgcaggcag gcatggacga
     1081 tcgcggtcag gccggcggcg acgttcattt cggggctgac gcggacacgg cccgccttgg
     1141 ccgccttgtc gaggccgcca ttggcgaggg cgaagccgcg ctcggccttg gtcatgacac
     1201 cgatcttcag cggaacgtcc ttggcgaggc tgcgggcggc atcgatcaaa gccgccggct
     1261 tgccgcgctt tagctcgagc tcgatctcgc tgagctccgc ctcgcgcttg ccgccgcgga
     1321 tcggaccgcg atcgaggatc atctcgatct cggccccatc atgggaaagc agccagattg
     1381 tccgctccac ctcggcggag acgatcggct tcagccgctt gcggagcttg gcgacgagcg
     1441 gcccgaccgg cgtttcggcg agcgcgtcga aatcgatctg gtcgccggca atctcccgct
     1501 cccattcggg ccggtcgaac aggccggcgg cgccatcctc ctgccgcttc acggtctgga
     1561 cgacccggtc gccggtgcgg cgcaggcgca acgaatagcc ggcggcgcgc aacgccccct
     1621 tgccgctgtc ataataagtg gagacctgcc gcgcccgccg cggctcgacc cgcttcacca
     1681 gtggatggtc gatgaggcgc gcggcatccg ccggatcgat ctccagcttc agctccacct
     1741 cattgccatc gctcatgtgc cagtccctcg aaaggcagcc cgactcaggc agcgcgcttg
     1801 gtagcttccc tttgtgacaa atcaaaagct aatccatgtt ggaaggtgcg cgctgccggg
     1861 tgaggaacgc cgcccgccgc ggcccgttgc ccgcggcgaa aggatgttca tgacccgcgc
     1921 catattgttc gacgtcgacg gcacgctcgt tgacagcaat gaccttcatg ccgccgcctg
     1981 gcgggaggca ttccgccatt tcgaggtcga gctttcgcat gaaaccgtgc gcggccagat
     2041 cggcaagggc ggcgacaacc tcatccccgc cttactgccg ccagacctcg tcgaaagccg
     2101 gcagaaggag atcgaggatt tccgatcgga cctgttcaag cgcgactatc tgccacgcgc
     2161 ggtgccgttt ccgggcgtgc gagcgctgtt cgagcggctc tatgccgacg gcgtcaagat
     2221 cgtgctcgca tcgtcggcca agtccgagga gctgcagttc cacctcaatt tgatcggcgc
     2281 cagcgacttc atctcggcca ccacctccca agacgatgtc gaaagctcga agcccgatcc
     2341 cgacattttc gcggcagcgc tcgccaaggt cgcgccgatc gcggcggagg atgcgttggt
     2401 ggtcggggac acgccctacg acgtcatcgc cgccgccagg gccggcctgc gcaccatcgc
     2461 gctgcgcagc ggcggcttcc ccgacgaggc gctgctcgga gcgggagcgg ccggacttta
     2521 caacgatgtc gaagacctgc tcgcccgcta cgacgagtgg ctcgcggccg ccggtcagaa
     2581 cagcttggcg atgccggcgt agagctggag gtcgggcgcc gcgcggctga gcccgatatt
     2641 ggcgccggcg tcgagctgaa ggtctgcgcg cggcgaccag ccggccgaca cgcccgccag
     2701 ccattcggtc gcttccgggt cgcggtcgcg gcgggcggcg acctcgacgg tggcgccgag
     2761 gctgtcgctc agcggaaggt cgagcccggc gatgccgcca taagcgaggt ggcgtccgtc
     2821 gcggtcgccg tcgaccgccg cctcgacggt ggcggtgaag ccgagcgcga agcctcgcgg
     2881 cagctcgtag ctcagcggca cgatcacgcc cgcgccccaa tcgccggcgc cgagcgcggc
     2941 ccctccggtc ggcagcgtcg cataggtcat gatggcgaca gccaggccgg agccgtcggg
     3001 gttctgcaaa ttctggcgca gcgcgatcag cacgtcgccg acgccgctcg tcgtctcgac
     3061 tgctccgccc gagcggaggc ggctgtggcc gaacaaggtc cagccggcct gcacctcgag
     3121 cgagccggtc agcccgtagc gcagcaggag atcgccgccc gcgacgagat cggcgcgcgc
     3181 gccgtcctcg cggtcgacgg tccagtcgat cacgccgagc tcgagcgcga agcggccgcg
     3241 atcgagggtg caggccggcg tcccgagccc cggccggtcc gggcagaagc cgcgctcatc
     3301 ctgcgcccgc aaagcctcgg ccggcagcag cgccgaagcc gcggcgacgg ccgccgagat
     3361 tggaaacatg cgcatcaccg gctcccccca gcccgcttgt taccggcgcg cgcggccgcg
     3421 ctcaaggcac ggcaccaacc gcggccgagg gagtttgacg cccatggcaa agaaaaagaa
     3481 agcggtcgtc gcggccgccg aggcggtgca ggaagtcgac gccggcgcgg ccgaggcggt
     3541 ggcgccttac cgcaaggcct tgccggtgcg ggcgctttcc tgggtgagcg aggtcggcga
     3601 ccaaccgccg ctgcgggcgc tttgcggggc gatgatcctg gcggggctgg ccggcggcgg
     3661 accgcggctg gcgcgggccg gcgggcgtgc cttgctggcg cacagcctcg ccaccggcgc
     3721 caagaatttc gtcaagcacc gcgtcgaccg cacccgtccc cggcaggtcg tggaaagcgc
     3781 cggcatcgcg gaccacaaga tgaaaccggg gcggaacgag gccaaggcgg agaccagctt
     3841 cccctcgggc cacagcgccg gagcgatcgc ggtggcgcgc ggcttcgccc gcgaatttcc
     3901 cgaatatcag gcccccgctt tggccgcggc cggcttggtc gcgctcgccc agatcccgcg
     3961 ctgcgcccat tatccgaccg atgtcggcgc cggcctcgcc atcggcctcg ccgccgaggc
     4021 cgccaccgac gccgcctgga acgccgcggc ttcgctcgtc gaagagcgat tagaggatgc
     4081 gaattcccgc tgaaccctta gccggtggcc tcaatggccg gcgggcgcgg cgcctttcgc
     4141 ctggcgggtg aacagcacca gcggcagcag caaggcgaag acgagcgcca ggctgacgaa
     4201 gatgttgttg aacgccatca ccgtcgcttc gcgctctacc atgccggcca gctgggccag
     4261 cgccgccctg gcgggatcgg cgacgccact cgccgccatc ctctgcgtca tctgatcgag
     4321 ccagccggga acgtcggcat ggcccgccga gacggcggcg gcgagctcgg tgcggtggag
     4381 gtcctgccct tcgcgaaaca gggtgttgat caccgccagc ccgagcgcgc cgccgagatt
     4441 gcgggtgagg ttgaacaggc cggacgcgtt ctggacccgg tccgccggca gcgtgccgag
     4501 cgcgatgccg gtcatcggga tcatgcacat catcaagccg cagccgcgca acgcctgcgg
     4561 caaagccagc tcccagaagc cgctttcggc ggtgagctgg gcattctgcc acatgccgaa
     4621 ggcggtgagg aggatgccga ggccgagcag gaagcggagg tcgaccttgg cctgcagctt
     4681 gccgacaatg ggcgcggtga ggaacatggc gaggcccgtc accaccatca cttcgccaat
     4741 ctgggtcgag ctgtagccgc gcacgccgcc gaggaagagc ggcatcagat agaccgagcc
     4801 gtacaggccg acgccggcga tcatcgacac gaggctgccg acgatgaagt tgcggttggc
     4861 gaagacccgc aggtcgacga tcggcgcggc ggcggtgaag gcgcgccaga agaagatcgc
     4921 gccgcccaat atcatcgcca ccgccatcat gaatacggtg tcgtcctgca gccagtcatg
     4981 cttgggccct tcctcgagca cgatctcgag gctgccgagg aacaccgcca tgccgaccag
     5041 gccccagatg tcgatgcggc ggagcaaagc cggctcggca tcgcgcagcg gactgaaccg
     5101 ccagaccaaa gcggcggcgg cgaggccggg gccgaggttg atcaggaaca gccactgcca
     5161 gcccatcgaa tcggtgatcc agccgccgag gctcgggccg accgagggcg ccagcgtcga
     5221 gaccatgccg accagcacga tcgccggcgc gcgcttcgcc tccgggaaca ggatgaacaa
     5281 ggcggccatg gtggtgggga tcatggcgcc gccgaggaag ccctgcaggg tacggaacag
     5341 gattaaggat tcgatgctcc acgcggtcgc gcaggcgagg ctcatcaggg tgaagccgag
     5401 cgcggagaaa gtgaataaag tgcgcatcga cagcgcgcgg ccgagcagcc ccgacaaggg
     5461 aatcgcgatc acctcggcga tcagatagct ggtctggacc caggcgatct cgtcggccga
     5521 ggcggaaacg ccggcctgga tctcgcgcag cgaggacgcg acgatctgga tgtccaggat
     5581 cgccatgaaa ttgccgacga tcatggcgag atagccggcc cagatcgcgc gcggcgtgta
     5641 ggagcgggga tcggtcacga gcaccgctcc ggctcgtcac ccctgatcgt gcaacaggcc
     5701 ctcacggctt agcccctggt gtccaccgtc gcttcgacgg acaggcccgg gcgcagcaac
     5761 tgggcggcga gcgcgtcctt ctcgaccacg atcctgaccg gcacgcgctg gacgatcttg
     5821 gtgaaattgc cggtcgcgct gtcggtgggg atgagcgcga attgggcgcc ggaggccggc
     5881 gcgaggcttt cgatgcggcc gcgcaccttg aggcccgggt aagcgtcaac ctcgagctcg
     5941 accggctggc cgacccgcat cttgccgagc tgggtctcct tgaaattggc gacgacatag
     6001 gcctgggcta cgggcacgat ggcgagcaat tgctggccgg ggcggacgag ctgcccctcg
     6061 cggaccgagc ggttgccgac cacgccggcg acgggcgcgc ggatcaccgt gcgggcgaga
     6121 tcgagcttga gctggcggag gttggcctcg gccgcctgca cctgcgcctg gccggcggcg
     6181 atgctcgcct cggctccggc ggtggcggcg acgagcgcgc cggcctggcc gcgctgcgcc
     6241 gagaccgcgg c
//
LOCUS       DASVRK010000280        16915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_303572, whole genome shotgun sequence.
ACCESSION   DASVRK010000280 DASVRK010000000
VERSION     DASVRK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16915
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303572"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1289)
                     /gene="pstA"
                     /locus_tag="VF589_11300"
     CDS             complement(15..1289)
                     /gene="pstA"
                     /locus_tag="VF589_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX8258209.1"
                     /translation="MTAIAKGGRTRAPTDWKTDEMQTRIRRRYQAERRFRLIGLGAVL
                     LSAGFLAFLLITMLGNGLRGFTQTEVRLDIDFPRSTLILDQATLRGFGADAALATADV
                     EGAMRAAAAKRYGDAADELFSQAAWLRVRERIKEDPAVLNGTETFWLPASDAIDVAAK
                     SEGAPEAEALHRRLSGAGAVSTGFNWNFLTGSDATDPTVVGIWGAFKGSLLTMLVTLA
                     LAFPIGVLSAVYLEEYAPRNRWTDLIEVSINNLAAVPSIIFGLLGLAVFLNFMHLPRS
                     APLVGGITLALMTMPVIVIAGRNAIKSVPPSIRDAALGIGASRVQVVFHHVLPLALPG
                     ILTGTIIGMARALGETAPLLMIGMRAFIAAPPSGISEPASVLPVQIFLWSDEVSRGFV
                     EKTSAAIIVLLIFLLEMNGFAIYLRNKFERRW"
     gene            complement(1315..2709)
                     /gene="pstC"
                     /locus_tag="VF589_11305"
     CDS             complement(1315..2709)
                     /gene="pstC"
                     /locus_tag="VF589_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX8258210.1"
                     /translation="MSLAAIALLIIGLGIVAWLSARARALAFAGPGAGLGRARSHSLP
                     SYHGWYVALWAVLPALAFLAVWSGVSGGLVTQAVLDSPAAAALPPEPMQRGAILSEAR
                     SIAAGTLQAGFNPMSSSLAPAYEQASNRYLLIGGGISLLLAFAGGAYAFTRVSPHFRA
                     RTRVERLVMAVLLIASLIAILTTLGIVLSLLFESLRFFARVSPIEFLFGLNWSPQTAM
                     RADQAGSSGAFGAIPLFWGTIFIGAIIAMIVAIPLGLMSAIYLTQYASPRVRAWMKPL
                     LEVLAGVPTVVYGYFAALTVAPAVRDFGVALGIPASSESALAAGLVMGIMIIPFVSSM
                     ADDSIAAVPQSMRDGSLAMGATTSETIKRVLVPAALPGVVGGVLLAVSRAIGETMIVV
                     MAAGLAARLTANPFESVTTVTTQIVQLLTGDQEFDSAKTLAAFALGLVLFIVTLLLNI
                     VALRVVKKYREAYD"
     gene            complement(2813..3868)
                     /locus_tag="VF589_11310"
     CDS             complement(2813..3868)
                     /locus_tag="VF589_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8258211.1"
                     /translation="MKKPILTLALAGLAIAGCQNQDGGAGGGDAGARQQIRIVGSSTV
                     YPFATAVAERFAQSNPSFKAPIVESTGTGAGMKLFCAGVGAQHPDITNASRRIKTSEL
                     EDCRKNGVNQVVEVPIGIDGLAFISAKNAQAIPLTVEDVYEAIAATPYGKPNTAKTWK
                     DVNPALPATPIQVYGPPPTSGTRDALAELILTKGCETNAEMKALKASDEDRHKEVCTK
                     VREDGLYVETGENDNLLVQKVTQNPGAVGVMGYSFLEENANAVQGLTLGGVVPTYQTI
                     SAFDYPGARPLYLYVKGEHVNAIPGLKEYLAEFARGWGEKGYLVQRGLISSPADVQAS
                     ATAAATSLKPVDAAQLK"
     gene            complement(3890..5176)
                     /locus_tag="VF589_11315"
     CDS             complement(3890..5176)
                     /locus_tag="VF589_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8258212.1"
                     /translation="MAPTRLWRPLTALAAVAIGLVSALSGGSPLAAAFAALMFGIVAG
                     SVIWAPAEAGPRAGAADDEPAPPPLPGAGDVIDAIEDPMLLVRDRRVVHANAAARAVL
                     GQHIDGVDVRLAIRHPAAAEQLISRGGDQTAERLTRIELVGLGERDRRWAMTILRLGD
                     GSRLVRLSDRSEAFASEQMRVDFVANASHELRTPLATLIGFAETLQDDATAEDRKTRH
                     RFLTIMSDEARRMQQLIDDLISLSRIEAERFSPPREAVPLLPLVEEVRVGCAQLIAEK
                     GSGIRVEQGVADPVVPGDRAQLLQMLRNLVINALKYGRQGEDVVIRFDPAGPEMVRMR
                     VIDRGEGIAQEHLPRLTERFYRVDPSRSRAMGGTGLGLAIVKHIAQRHRGRLDIASRP
                     GEGTAVSVYLPALSSKSHRTVTEVSRKANRDDPDAP"
     gene            5276..5683
                     /locus_tag="VF589_11320"
     CDS             5276..5683
                     /locus_tag="VF589_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258213.1"
                     /translation="MSDKFDHQQDDAASRPSRRVVLALGAAAASTVVTIRPALAQTAG
                     SVLTCEIPVPAPEAAGQYIAADGSLVAPGTSGAFAPPLRNITGEEAKVLLRHGAAPPG
                     MDSEAAQAYANYIRRLQAGMSGFTCYASLQMPR"
     gene            5698..6042
                     /locus_tag="VF589_11325"
     CDS             5698..6042
                     /locus_tag="VF589_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HEX8258214.1"
                     /translation="MAGPCYIGEPPATLRRVELDGLTAFFHSPSGMTHILAPPAPQIL
                     ELLAAGPADAGELYAQLSERFEISGGVAELQSRLDELQAAGLVSHCSAAEAGARAEAA
                     LANPPPAVARAA"
     gene            6039..6905
                     /locus_tag="VF589_11330"
     CDS             6039..6905
                     /locus_tag="VF589_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HEX8258215.1"
                     /translation="MRHEHAIQVGPVGFRIGSDWPDPIRDLRSLYAGYPEPDGACDFT
                     VRLEATKPWRRWINPSVAISGDFTLPDAAPLPLAQGLLAAEMAMNLQMALGQKRYLLL
                     HAATVEKAGRALILTGHSGSGKSTLAAILAERGWRLMGDEFALLDPETGRLHAFPRAI
                     SLKNGSISVVAGIADHSRLGPLIQGTPKGAIRHLRPNREALDRMGEPAEPALLLFPRF
                     GRGLHAEVRPVGAAEAFVRLTQASTNYVALGEGGFSALTELVTGIPACAIDYPDTEAA
                     LGLVHRLWTELA"
     gene            6902..7912
                     /locus_tag="VF589_11335"
     CDS             6902..7912
                     /locus_tag="VF589_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8258216.1"
                     /translation="MKSARLLAAALGDPGSTASLGPRQWTALIAAARAESLAGVLACR
                     LEGEALPAKVARILEDARRDHDHARTQALWEAEMCRRALAPLGLPVILLKGTAYAAAG
                     LPAGRGRSIGDLDILVPQEGLDRVEAALLAAGWEWVKPDGYDDLYYRRWMHELPPLIH
                     RDRDRMIDVHHTILPLTARPTPDAAALIAASVELPNGLRVLAPADMVVHAACHLFADG
                     DIAGGLRNLWDIDRMLRDFGARPGFWPELAERARLHQLAGPVGRALRLAAKLYGTPVD
                     AAVAGRAVPFDRLVEARLLARNGWGQTTGKALRFAFYLRSHWLRMPPLMLARHLWVKA
                     RR"
     gene            complement(7915..10263)
                     /locus_tag="VF589_11340"
     CDS             complement(7915..10263)
                     /locus_tag="VF589_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8258217.1"
                     /translation="MADDPAATPSGDPLDRDRIARLVVDSALDFAIFTTGVDGKVTSW
                     SPGAERLLGWPEAEILGRDSCIIFTPEDIEAGACEREARTASVEGRAEDERWHVRRDG
                     SRFWASGLMMRLDDEASGAQIGLVKILQDRTRQHEANEQLLALHGRSAEILESIADAF
                     YAVDADWRFTYVNRRAEEWWARDRAELIGKVYWDEFPQAVGSEPYRAHLKVMEERRPF
                     RLEALSPIIGRWLEIDIHPTAAGGLSVYFRDISERRAREARLRESEARLRLAVDAAGM
                     AIWEYDAATERLKLSPDLAAFLGLSPEELEDIDAVRSRYYPGDGSRLRAEAEAAFVRG
                     DRHFEAEFRFWRAPDDLRWFMLRGEIMIGASGEPERVLGAVVDITSRKSAEAALQQLN
                     ETLEEQVSQRTRDLLRAEEALRQSQKMEAIGQLTGGVAHDFNNLLTIIRSSVDLLRRP
                     GFSEEKKGRYLNAISETVDRAAKLTGQLLAFARRQALEPEIFDAPERTRRIADMVATI
                     VGARVAVEIDAECDTCFVEADAAQFETAIVNLTVNARDAMDGEGMLRIRIATVHGLPG
                     IRGHHGSEGAFVAVSVSDTGAGIPPEHLPHIFEPFFTTKEVGKGTGLGLSQVYGFAKQ
                     SGGDVEVESAPGEGTTFTLYLPRVSPAAGKREARGAASDRPDPAIRILVVEDNVQVGE
                     FASQLLEELGHTTTLAPNALKALEILMARHGEFDLVFTDVVMPGMSGIDLAEEVRRLY
                     PGLRVILTSGYSHVLAEQGTHGFELLQKPYSVDGVTRLIRAG"
     gene            10397..11755
                     /gene="glmU"
                     /locus_tag="VF589_11345"
     CDS             10397..11755
                     /gene="glmU"
                     /locus_tag="VF589_11345"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX8258218.1"
                     /translation="MTRTKPFAAIILAAGKGTRMKSDVHKVLHPIAGRAMLDHLLGVL
                     DGLGPARKIVVVGSGREQVQHLVDARGGQVVVQDQQLGTAHAVRQAEGALGDFDGDVL
                     ILYGDTPLIEPETMAGMLDRLNAPDAPTVVVVGFRPVDPLAYGRILAAPDGTIEKMVE
                     YKDATPEERAVDLCNSGLMAARAADLFPLLGRVGNANAAGEYYLPDIVMLAAADGRRS
                     AVIEAQPWEMAGVNSRAELALVEAEWQRRRRLAAMADGVTLVAPDTVWFAHDTEIGRD
                     SVVEPNVVFGPGVWVGEGVCIRAFSHIEGAHIAAGAEIGPYARLRPGADIGEKAKVGN
                     FVEVKKARLGPGAKASHLSYLGDADIGAGANIGAGTITCNYDGYFKYPTVIGEGAFIG
                     SNSALVAPVTIGANAIVGAGSVVTRDVAPEALAIARGEQSERPAWAARFRAIMSARKK
                     TG"
     gene            11843..13666
                     /gene="glmS"
                     /locus_tag="VF589_11350"
     CDS             11843..13666
                     /gene="glmS"
                     /locus_tag="VF589_11350"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX8258219.1"
                     /translation="MCGIIGIVGHGDVAGRLMDGLKRLEYRGYDSAGLCTIVDGRLER
                     RRAEGKLENLARELASEPLPGQVGIAHTRWATHGAPTRDNAHPHATAEVALVHNGIIE
                     NFRPLREELVAEGRSFTSQTDTEVVAHLVSREIERGASPKDAVARTLKRLHGAFSLAI
                     LIRGYPDMLIGARLGSPLVIGYGEDETYLGSDALALAPLTQKISYLEEGDWAIVTRDG
                     TQVYDRDDNPVTRPITHSGASAAAVEKGNHRHFMQKEIFEQPIVVAQTLQSYLRPLEQ
                     KVALPDMDFSFADVPRITIVACGTSYYAGMVAKYWLEQFARVPVELDIASEFRYRQPV
                     LEEGGLALFISQSGETADTLAALRHARAQGQRIAVVVNVPTSSMAREADLLLPTHAGP
                     EIGVASTKAFTCQLAVLAALAAKVARDKGRMTEEEEREIVGHLTEAPAAMNAALNHDE
                     EIEAMAPVIARARDVLYLGRGPDYPMALEGALKLKEISYIHAEGYAAGEMKHGPIALI
                     DDDVPVIVIAPSGPLFEKTVSNMQEVQARGGKVVLISDAEGIAAAGEGTIATIEMPKV
                     HPLIAPMVYAIPVQLLAYHVAVVKGTDVDQPRNLAKSVTVE"
     gene            complement(13692..14363)
                     /locus_tag="VF589_11355"
     CDS             complement(13692..14363)
                     /locus_tag="VF589_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258220.1"
                     /translation="MLLAAAVALLVGAAPPTPPLVFGAQSRQGLVVVYTRIAPGPKVP
                     EPHLLFVPYDPATKTIESRRRDLPGKPFKVGRGAGLWSNADRQNWQGWAVLAEPGIYV
                     AAQATNPNSGGSSNSYARGTLMFEVKPGRALYVGDYSFHLGKWKFPNAGQVAGDFLKG
                     FFTLGLVMPKQAVYSGDVSYDEARAKAYLATRLPQPVELERAPLKYVDFADSSRRAYY
                     LNPIG"
     gene            complement(14448..14900)
                     /locus_tag="VF589_11360"
     CDS             complement(14448..14900)
                     /locus_tag="VF589_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258221.1"
                     /translation="MKTLTIAAAALLAAAPALAEAPPAAKADAKPAAVTIPIDTGSYV
                     NVGESCGDPINALHYDGARLRWLYADATQSFAETPRSVRQEGRVYTLESMESFEMPSN
                     SDEPEMAPAFTRIEVLGRTRIRISVQDDVEARLCAPAELPEGIRRQIG"
     gene            complement(15033..16301)
                     /locus_tag="VF589_11365"
     CDS             complement(15033..16301)
                     /locus_tag="VF589_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258222.1"
                     /translation="MSFYQRMYRGTEANEIIRGSALNDDIHGIAGHDQLFGLGGDDAL
                     NAGEHGDTMWGGDGDDFLGGSLESDHMYGEAGADRLFGLLGGDFMYGGAGMDFLVGEA
                     GADYMSGGDQADELHGGGDDDTMLGGRGHDRMAGDAGNDHMDGGSGDDSLSGGSDHDH
                     LDGGAGDDELDGEDGDDWIDGGLGADDIAGGIGADTIFGGAGADTVMAGEGNDVVDGG
                     EDSDRLYGDGGDDLLSGGAGYDELYGGVGSDFLDGGAGGDYLEGFDGQDRLSGFAGND
                     VIDGGSGMDVLVGGSGNDRLFAGAGDDILTGGLGTDLLAGDAGADQYRFLDGHAGTAP
                     SLADTIEGFSREEGDVMDLSRVDAATGAGNDAFSFIGDGAFSGTAGALRYESNGLGVM
                     VEADRDGDSMADFFIQVSGPIELAASDFVL"
     gene            complement(16591..>16915)
                     /locus_tag="VF589_11370"
     CDS             complement(16591..>16915)
                     /locus_tag="VF589_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258223.1"
                     /translation="ILTATAAISTMAMTAPAAAQNQAGLVNVNISDLNLENIANNLSV
                     EVSQVPVNVQVPVSVAANICGVAVNALATQKKNGPVSCDAASGSEAAVTQALSKQMRK
                     SNKSK"
BASE COUNT         2629 a         5805 c         5838 g         2643 t
ORIGIN      
        1 gtctgtcatg agtttcacca gcgtctctcg aacttgttgc gcagatagat ggcaaagcca
       61 ttcatctcga ggaggaagat gaggaggacg atgatcgcgg cggatgtttt ctcgacgaaa
      121 ccgcggctga cctcgtccga ccagagaaag atctggaccg gaaggaccga ggcgggctcg
      181 gaaatgccgg acggcggtgc agcgatgaag gcgcgcatgc cgatcatcag cagcggcgcg
      241 gtctcgccca aagcgcgggc catgccgatg atggtgccgg tgaggatgcc gggcaaagcc
      301 agcggcagca cgtggtggaa gacgacctgg acgcggctgg cgccgatgcc gagcgccgcg
      361 tcgcggatcg acggcggcac cgatttgatc gcgttccttc cggcgatgac gatcaccggc
      421 atggtcatca gggcaagcgt gatgccgccg accagcggcg ccgaacgtgg caagtgcatg
      481 aagttcagga acacggcgag gccgagcagg ccgaagatga tcgacggcac cgcggcgaga
      541 ttgttgatcg acacttcgat gaggtcggtc cagcggttcc gcggcgcata ttcctcgaga
      601 tagacggccg acaggacgcc gatcgggaag gcgagcgcga gcgtcaccag catggtcagc
      661 agggagccct tgaacgcgcc ccagatgccg acgacggtcg ggtcggtggc gtcggaaccg
      721 gtgaggaagt tccagttgaa gccagtgctg accgcgcccg cgccgctcag gcggcgatgg
      781 agcgcttccg cttcgggggc gccctcgctc ttggcggcga cgtcgatcgc gtccgaggcg
      841 ggcagccaga aggtctcggt gccgttcagc accgccggat cctccttgat ccgctcgcga
      901 acgcgcagcc aggcggcctg ggagaagagc tcgtcggcgg catcgccata gcgcttggcc
      961 gccgccgcgc gcatcgcgcc ttcgacatcg gcggtggcca aagcggcgtc ggcgccgaag
     1021 ccgcgcaagg tggcttggtc gaggatcagg gtgctgcgcg ggaaatcgat gtcgagccgc
     1081 acctcggtct gggtgaagcc gcgcaggccg ttgccgagca tggtgatgag caggaaagcg
     1141 aggaagcccg ccgagagcag gaccgcccca agtccgatca gccggaagcg ccgctcggcc
     1201 tgatagcggc ggcggatgcg ggtctgcatt tcgtccgtct tccagtcggt cggcgcgcgg
     1261 gtgcgtccgc cctttgcgat ggccgtcatg cccgggctct cggttccgct tcggtcagtc
     1321 ataagcttcg cgatattttt tgacgacgcg cagggcgacg atgttgagca gcaaggtgac
     1381 gatgaacagc accagcccaa gcgcgaaggc ggccagcgtc ttggcgctat cgaactcctg
     1441 gtccccggtc agcaattgca cgatctgggt ggtgacggtg gtgacgcttt cgaacggatt
     1501 ggcggtgagc cgcgccgcca gccccgccgc catgacgacg atcatggtct cgccgatcgc
     1561 gcggctcacc gcgagcagca cgccgccgac cacgcccggc agcgccgccg gcaccagcac
     1621 gcgcttgatc gtctccgacg tggttgcgcc catcgccagc gatccgtcgc gcatcgattg
     1681 cggcaccgcc gcgatcgaat cgtcggccat cgaggagacg aacgggatga tcatgatgcc
     1741 catgacgagc ccggccgcga gcgccgattc ggacgaggcg gggatgccga gcgcgacccc
     1801 gaaatcgcgc accgccgggg ccacggtcag cgccgcgaaa tagccgtaga ccacggtcgg
     1861 cacgcccgcg agcacttcga gcaacggctt catccaggcg cgcacgcgcg ggctggcata
     1921 ttgggtgagg tagatggcgc tcatcaggcc gagcgggatg gcgacgatca tggcgatgat
     1981 cgcgccgatg aagatggtgc cccagaaaag cgggatcgcg ccgaacgcgc cggacgatcc
     2041 ggcctggtcg gcgcgcatcg cggtttgcgg gctccagttg agcccgaaca ggaattcgat
     2101 cggcgagacc cgcgcaaaga agcgcagcga ttcgaacagc agcgacagga cgatgccgag
     2161 cgtggtcagg atcgcgatca aagaggcgat cagcagcacc gccatcacca gccgctcgac
     2221 ccgggtgcgg gcgcggaaat ggggggagac gcgggtgaag gcgtaagcgc cgcccgcgaa
     2281 ggcgagcaag agggagatgc cgccgccgat cagcagatag cggttcgacg cctgctcgta
     2341 agccggggcg aggctgctcg acatcgggtt gaagccggcc tggagggtgc cggcggcgat
     2401 ggagcgggct tcgctgagga tggcgccgcg ctgcatcggc tcgggcggga gcgcggcggc
     2461 ggccgggctg tcgagcaccg cctgggtgac gagcccgccc gacacgccgc tccagacggc
     2521 gaggaaggcg agcgccggca gcaccgccca caaagcgaca taccagccat gataggaggg
     2581 gagcgagtgc gaccgcgccc ggcccaggcc cgcgcccggc ccggcaaagg ccaaagcgcg
     2641 cgcgcgggcg ctgagccagg cgacgatgcc cagcccgatg atcagcaatg cgatcgctgc
     2701 cagcgacatt cggaacctta cccggccggg ccccggccga agccggggcc cagacctttg
     2761 gcccgccggg ccccggcttt ggccggggcg caggaacggc cgtcttccac gcttacttca
     2821 actgcgccgc gtcgaccggc ttcagcgagg tcgccgccgc ggtcgcgctc gcctggacgt
     2881 cggcgggcga ggagatcagg ccgcgctgga ccagatagcc tttctcgccc cagccgcgcg
     2941 cgaactcggc cagatattcc ttgaggccgg ggatcgcgtt cacatgctcg cccttcacgt
     3001 acaggtagag cgggcgcgcg cccggatagt cgaacgcgga gatggtctga taggtcggaa
     3061 ccacgccgcc cagcgtcagg ccctgcaccg cgttcgcatt ctcctcgagg aagctgtagc
     3121 ccatcacccc gaccgcgccg ggattttgcg tgaccttctg gacgagcaga ttgtcgttct
     3181 cgccggtctc gacatagagg ccgtcctcgc ggaccttggt gcagacctcc ttgtgccggt
     3241 cctcgtcgct cgccttgagc gccttcatct cggcattggt ctcgcagccc ttggtgagga
     3301 tgagctcggc cagcgcatcg cgggtgccgg aggtcggcgg cgggccatag acctggatcg
     3361 gcgtcgcggg cagcgccgga ttgacgtcct tccaggtctt ggcggtgttg ggcttgccat
     3421 aaggcgtcgc cgcgatcgcc tcatagacgt cctcgacggt gagcgggatc gcctgggcgt
     3481 tcttcgcgga gatgaaggcc aggccgtcga tcccgatcgg aacctcgacc acctggttga
     3541 cgccattctt gcggcaatcc tcgagctccg aggtcttgat ccgccgcgag gcgttggtga
     3601 tgtcgggatg ctgcgcgccg acgccggcgc agaacaattt catgcccgcg ccggtgccgg
     3661 tcgattcgac gatcggcgcc ttgaacgacg ggttggactg ggcgaaacgc tcggcgaccg
     3721 cggtcgcgaa cggatagacg gtcgaggatc cgacgatccg gatctgctgc cgcgctccgg
     3781 catccccgcc cccggcgccg ccatcctggt tctggcagcc ggcgatcgcc agccccgcca
     3841 gggccagagt aagaatcggt tttttcaaat ttgcctccat cgcaacgcgc tatggcgcgt
     3901 ccggatcgtc cctattggcc ttgcgggaga cttctgtgac ggttcggtga ctctttgatg
     3961 acaaggcggg caggtagacg ctgaccgccg tcccttcgcc tggccggctc gcgatgtcga
     4021 gccgcccgcg gtggcgctgg gcgatatgct tgacgatggc aaggccgagg ccggtcccgc
     4081 ccatcgcccg gctgcggctc ggatcgaccc ggtagaaacg ctcggtgagt cgcggcagat
     4141 gctcctgggc gatgccttcg ccgcggtcga tcacgcgcat ccgcaccatc tcgggcccgg
     4201 ccgggtcgaa gcggatcacg acatcctcgc cctggcggcc atatttgagc gcgttgatga
     4261 cgaggttgcg gagcatctgg agcaattgcg cgcggtcgcc cggcacgacc gggtcggcca
     4321 ccccctgctc gacccggatg ccgctccctt tctccgcgat gagctgcgcg cagccgaccc
     4381 gcacctcctc gaccaaaggc agcaggggca ccgcctcgcg cggggggctg aagcgttcgg
     4441 cctcgattcg cgagagggag atgaggtcgt cgatcagctg ctgcatccgc cgcgcctcgt
     4501 ccgacatgat ggtgaggaag cggtggcggg tcttgcggtc ctcggcggtg gcgtcgtcct
     4561 gcagcgtctc ggcaaagccg atcagggtgg cgagcggcgt gcgcagctcg tggctggcat
     4621 tggcgacgaa atcgacccgc atctgctccg aggcgaaggc ttcgctgcgg tccgaaagcc
     4681 gcaccagccg gctgccgtcg ccgagccgca atatggtcat cgcccagcgc cggtcgcgct
     4741 cgccgaggcc gaccagctcg atccgggtga gccgctcggc cgtctggtcg ccgccgcggc
     4801 tgatcaattg ctcggccgcg gccgggtggc ggatggcgag gcggacgtcg acgccgtcga
     4861 tatgctggcc cagcaccgcc cgcgccgccg cattggcatg gacgacgcgc cggtcgcgga
     4921 ccagcagcat cggatcttcg atcgcgtcga tgacgtcgcc ggcgccgggc agcgggggag
     4981 gggcgggctc gtcgtcggcc gcgccggcgc gggggcccgc ttcggccgga gcccagatca
     5041 ccgatccggc gacgatccca aacatcaagg cggcgaaggc ggcggcgagc ggcgaacccc
     5101 cggacagcgc cgagacgagg ccgatcgcga cggccgccag cgcggtgagg ggtcgccaaa
     5161 gacgcgtcgg ggccattccg actgtccctt agcgggatag tggccgctac gccataactc
     5221 gaaagttgaa ggctttacgc ttatgaaagg agcccgcccg taagtagatt gtgtcatgag
     5281 cgacaagttc gaccaccaac aggatgatgc ggcttcccgg ccgagccgcc gggtcgtgct
     5341 cgccctcggc gccgccgccg cctcgacggt ggtgacgatc cgcccggccc tggcccagac
     5401 cgccggctcc gtcctcacct gcgagatacc ggtccccgct cccgaagcgg ccggccagta
     5461 tatcgccgcc gacggcagcc tggtcgcacc cggcacctcg ggcgcgttcg cgccgccact
     5521 tcgcaacatt accggcgaag aggcgaaggt gctgctgcgc cacggagccg cgcctccggg
     5581 catggatagc gaagccgctc aggcctatgc caactatatt cgccgtctgc aggcggggat
     5641 gagcggtttc acctgctacg cctcgctgca gatgccgcgc tgaccggcac ggccgcgatg
     5701 gccggcccct gttacatagg cgagccgccc gccacgctgc gccgcgtcga gctcgacggc
     5761 ctcaccgcct ttttccattc gccttcgggc atgacccaca tcctcgctcc gccggcgccg
     5821 cagatcctcg agctgctcgc ggcgggaccg gccgatgccg gcgagcttta cgcgcagttg
     5881 agcgagcggt tcgagattag cggcggcgtg gccgagctcc agtcgcgcct cgacgagctt
     5941 caggccgccg gcctggtcag ccattgctcc gcggcggaag cgggagcccg ggccgaagcc
     6001 gcactcgcga atccgccgcc ggcggtcgcc cgcgccgcat gaggcacgaa catgccatcc
     6061 aggtggggcc ggtcggcttc cgcatcggct cggattggcc ggacccgatc cgcgacttgc
     6121 gcagcctcta tgccggctat cccgagcccg atggggcgtg cgacttcacc gtccggctcg
     6181 aagcgaccaa accgtggcgg cgctggatca atccctccgt ggcgatcagc ggcgatttca
     6241 cgcttcccga cgccgcgccg ctgccgctcg cccaggggct gctcgccgcc gaaatggcga
     6301 tgaacctgca aatggcgctt ggccagaaac gctatctgct gctccacgcg gcgacggtgg
     6361 agaaagcggg gagggcgctg atcctcaccg gccattcggg atcgggcaag tcgacgctgg
     6421 cggcgatcct ggccgagcgc ggctggcgcc tgatgggcga cgaattcgcc ttgctcgacc
     6481 cggagaccgg ccgcctccac gccttcccgc gcgcgatcag cctcaagaac ggctcgatct
     6541 cggtcgtggc cggcatcgcc gaccatagcc ggctggggcc gctcatccag ggcacgccga
     6601 aaggcgcgat ccggcacttg cgccccaatc gcgaagcgct cgaccggatg ggcgagccgg
     6661 cggagcctgc cttgctgctg ttcccgcgct tcgggcgcgg ccttcacgcc gaggtgcggc
     6721 cggtcggcgc cgccgaggcg ttcgtgcggc tgacccaggc ctcgaccaat tatgtcgcgc
     6781 ttggcgaagg tggcttttcg gcgctgaccg agctggtgac cggcatcccg gcctgcgcga
     6841 tcgactatcc cgacaccgag gccgcgctcg ggctggtcca tcggctgtgg acggagctgg
     6901 catgaaatcg gcgcgacttc tcgcggccgc gctcggcgat ccgggcagca cggcctcttt
     6961 gggcccgcgc cagtggaccg ctttgatcgc cgcggcgcgc gcggaatcgc tggccggcgt
     7021 gctcgcttgt cggctggagg gcgaggcgct tccggccaag gtcgcccgga tcctcgaaga
     7081 tgcccgccgc gaccatgacc atgcccgcac ccaggccttg tgggaggcgg agatgtgccg
     7141 ccgcgcgctg gcgcctctgg ggctgccggt gatcctgctc aagggcaccg cttatgccgc
     7201 cgccggcctt ccggcggggc gggggcgctc gatcggcgac ctcgacattt tggtgccgca
     7261 ggaggggctc gaccgggtcg aggcggcgct gctcgcggcc ggctgggaat gggtcaagcc
     7321 ggacggctat gacgatctct attaccgccg ctggatgcac gagctgccgc cgctgatcca
     7381 ccgcgaccgc gaccgcatga tcgacgtcca ccacaccatc cttccgctga ccgcgcggcc
     7441 gacgcccgat gcggccgcgc tgatcgccgc cagcgtcgag cttccgaacg ggctgcgcgt
     7501 gctggcgccg gccgacatgg tggtccacgc cgcctgccac ctgttcgccg acggcgacat
     7561 tgccgggggc ctacgcaacc tgtgggacat cgaccggatg ctgcgcgatt tcggcgcgcg
     7621 gcccggcttc tggcccgagc tcgccgagcg ggcgcggctc caccagctcg ccggcccggt
     7681 cgggcgcgcg ctgcggctcg cggcgaagct ctacggcact ccggtcgatg cggccgtcgc
     7741 cggtcgggcg gtaccgttcg accggttggt cgaggcgcgg ctcctcgccc gcaacggctg
     7801 gggccagacg accggcaagg cgcttcgttt cgctttctac ctgcgctcgc actggctgcg
     7861 catgccgccg ctgatgctgg cccgccacct gtgggtgaag gcgcgccgct gatctcagcc
     7921 ggcgcggatg agccgggtca cgccgtcgac cgaatagggc ttttgcagca gctcgaagcc
     7981 gtgggttccc tgctccgcca gcacatggct gtagccgctg gtgaggatca ccctcagccc
     8041 cggataaagc cgccgcactt cctcggcgag gtcgatcccg ctcatccccg gcatcaccac
     8101 gtcggtgaac acgaggtcga actcgccgtg ccgcgccatg aggatttcga gcgccttcag
     8161 cgcgttcggg gccagcgtgg tcgtatggcc gagctcttcg agcagctggc tcgcgaactc
     8221 gccgacctgg acattgtcct cgaccaccag gatgcggatc gccgggtcag gccggtccga
     8281 ggcggcgccg cgcgcttcgc gcttgccggc ggcgggcgac acgcgcggca aatagagggt
     8341 gaaggtcgtg ccttcgcccg gcgcgctttc cacttcgaca tcgccgcccg attgcttggc
     8401 gaagccatag acctgggaga ggccgaggcc ggtgcccttg cccacttcct tggtggtgaa
     8461 gaagggttcg aaaatgtggg gcagatgctc cggcgggatg ccggcgccgg tgtcgctgac
     8521 cgagaccgcg acgaacgcgc cttcgctgcc atgatggccg cggatgccgg gcagcccgtg
     8581 cacggtggcg atgcggatgc gcagcatgcc ctcgccgtcc atggcgtcgc gggcgttcac
     8641 cgtgaggttg acgatcgcgg tctcgaactg ggcggcgtcg gcctcgacga agcaggtgtc
     8701 gcactcggcg tcgatctcga ccgcgacccg ggctccgacg atggtggcaa ccatgtcggc
     8761 gatgcgccgg gtgcgctcgg gggcgtcgaa gatttccggc tccagcgcct ggcggcgggc
     8821 gaaggcgagc aactggccgg tcagcttggc ggcgcggtcg accgtctccg aaatggcgtt
     8881 gaggtagcgg cctttcttct cctcgctgaa gccgggccgc cgcagcagat cgaccgagga
     8941 gcggatgatg gtcagcagat tgttgaaatc atgcgcgacg ccgccggtca gctggccgat
     9001 cgcctccatc ttctgcgact ggcggagcgc ttcttcggcg cgcagcaagt cgcgggtgcg
     9061 ctggctgacc tgctcctcca gcgtctcgtt caattgctgc agcgcggctt cggcggactt
     9121 gcgcgaggtg atgtcgacga cggcgccgag cacgcgctcg ggctcgcccg aagctccgat
     9181 catgatctcg ccgcgcagca tgaaccagcg caggtcgtcc ggcgcgcgcc agaagcggaa
     9241 ctcggcctcg aaatggcggt cgccgcgcac gaaggccgct tccgcctcgg cgcgtagccg
     9301 gctgccgtcg cccggatagt aacggctcct gaccgcgtcg atatcctcca gctcctcggg
     9361 gctcaggccg aggaaggcgg cgaggtcggg cgacagcttc agccgttcgg tggcggcgtc
     9421 atattcccag atcgccattc cggccgcgtc gaccgccagc cgcagccgcg cctcgctttc
     9481 gcgcagccgc gcctcgcgcg cgcggcgctc ggaaatgtcg cggaaataga ccgaaagtcc
     9541 gcccgcggcg gtcgggtgga tgtcgatctc cagccatcgg ccgatgatcg gcgagagcgc
     9601 ttcgagccgg aacggccggc gctcctccat caccttgagg tgcgcgcgat agggttcgct
     9661 gccgaccgcc tgcgggaatt cgtcccaata gaccttgccg atcagctcgg cccggtcgcg
     9721 cgcccaccat tcttccgccc tgcggttgac gtaggtgaag cgccagtcgg catcgaccgc
     9781 gtaaaaggcg tcggcgatgc tttcgaggat ctcggcgctg cgcccgtgga gcgcgagcag
     9841 ctgctcgttg gcctcgtgct ggcgggtgcg gtcctgcagg atcttgacca ggccgatctg
     9901 ggcgccgctg gcctcgtcgt cgagccgcat catcaggccc gacgcccaga agcggctgcc
     9961 gtcgcgccgg acgtgccagc gctcgtcctc ggcgcggccc tcgacgctcg ccgtccgggc
    10021 ctcgcgctcg cacgccccgg cctcgatatc ctcgggcgtg aagatgatgc agctgtcgcg
    10081 gcccaatatc tcggcctcgg gccagccgag cagccgctcg gcgcccgggc tccagctcgt
    10141 caccttgccg tcgacgccgg tggtgaagat cgcgaaatcg agcgcgctgt ccaccacgag
    10201 gcgcgcgatc cggtcccggt cgagcgggtc gcccgaaggc gtggcggcgg ggtcatcggc
    10261 catcgcctac aagaacaggc ggcagtcggg gccgttccgt agcggagcca acggccgcgc
    10321 cggagccaag tgccgggggc gctatggaaa tagcaagatt tacatgcgat gggattggcg
    10381 aagccgggat ttaccaatga cgcgtaccaa gcctttcgcc gccatcattc tcgctgccgg
    10441 caagggcacg cggatgaaat ccgacgtcca caaggtgctg catccgatcg cggggcgggc
    10501 gatgctcgac catctgctcg gcgtgctcga cgggctcggc ccggcccgca agattgtcgt
    10561 cgtcggcagc ggccgcgagc aggtccagca tctcgtcgac gcgcgcggcg gccaggtcgt
    10621 cgtccaggac cagcagctcg gcaccgccca tgccgtccgc caggcggagg gagcgctcgg
    10681 cgacttcgac ggggacgtgc tcatcctcta cggcgatacg ccgctgatcg agcccgagac
    10741 gatggccggc atgctcgacc ggctgaacgc gcccgacgcg ccgaccgtgg tggtggtcgg
    10801 cttccgcccg gtcgatccgc tcgcttacgg ccgcatcctc gccgcaccgg acggcacgat
    10861 cgagaagatg gtcgaatata aggacgcgac gcccgaggag cgcgccgtcg atttgtgcaa
    10921 ttcgggcctg atggccgcgc gcgccgccga cctgttcccg ctgctcggcc gggtcggcaa
    10981 cgccaatgcc gccggcgaat attatcttcc cgacatcgtc atgctggccg ccgccgacgg
    11041 ccgccgctcg gcggtgatcg aggcccagcc gtgggagatg gcgggcgtca acagccgcgc
    11101 cgagctcgct ctggtggagg ccgaatggca gcgccgccgc cggctggcgg cgatggccga
    11161 cggcgtcacc ctggtcgccc ccgacacggt ctggttcgcg cacgataccg agatcggccg
    11221 cgacagcgtg gtcgagccca acgtcgtgtt cggccctggt gtgtgggtgg gcgagggcgt
    11281 gtgcatccgc gccttcagcc atatcgaagg cgcccatatc gccgccggcg ccgagatcgg
    11341 cccctatgcg cgcctgcgcc ccggcgccga catcggcgag aaggccaagg tcggcaattt
    11401 cgtggaggtg aagaaagcca ggctcggccc cggcgccaag gccagccatc tctcctacct
    11461 cggcgacgcc gacatcggcg ccggcgccaa tatcggggcc ggcaccatca cctgcaatta
    11521 tgacggctat ttcaaatatc cgaccgtcat cggcgagggc gccttcatcg gctccaacag
    11581 cgcgctggtc gcgcctgtca cgatcggcgc caacgccatc gtcggcgccg gctcggtcgt
    11641 cacccgcgac gtcgcccccg aagcgctcgc gatcgcgcgc ggcgaacaga gcgagcgccc
    11701 cgcctgggcc gcgcggttcc gcgcgatcat gtcggcgagg aagaaaacag gttagggatt
    11761 ccgtccgccc tgatggtcga aggccgccct gagcggcgcc tgggaccgct cggctcgaac
    11821 ggacttggag gtagtggatt ttatgtgcgg catcatcggc atcgtcggcc acggagacgt
    11881 cgccgggcgg ctcatggacg gcctgaagcg gctcgaatat cgcggctatg acagcgcggg
    11941 cctgtgcacc atcgtcgacg gccggctgga gcgccggcgg gccgagggca agctcgaaaa
    12001 cctcgctcgc gagctcgcct ccgagccgct gcccggccag gtcgggatcg cccatacgcg
    12061 ctgggcgacc cacggcgcgc cgacccgcga caacgcccac ccgcacgcca ccgccgaagt
    12121 ggcgctcgtc cataacggca tcatcgagaa tttccggccg ctgcgcgagg aattggtcgc
    12181 cgaaggccgc agcttcacca gccagaccga taccgaggtc gtcgcccatc tggtcagccg
    12241 cgagatcgaa cgcggcgcct cgcccaagga cgccgtcgcc cgcaccctga agcgcctcca
    12301 cggcgcgttc agcctcgcca tcctgatccg cggctatccc gacatgctga tcggcgcgcg
    12361 tctgggctcg ccgctggtga tcggctatgg cgaggacgag acctatctcg gctcggacgc
    12421 gctggcgctg gcgcccctca cccagaaaat ctcctacctc gaggaaggcg actgggcgat
    12481 cgtcacccgc gatggcaccc aggtctacga ccgggacgac aatccggtca cccgccccat
    12541 cacccattcc ggcgccagcg cggccgcggt cgagaagggc aatcatcgcc acttcatgca
    12601 aaaggagata ttcgagcagc cgatcgtggt cgcgcagacg ctgcaatcct atctccgccc
    12661 gctcgagcag aaggtggcgc tgcccgacat ggacttcagc ttcgccgacg tgccgcgcat
    12721 caccatcgtc gcctgcggca cctcttatta tgccggcatg gtcgccaaat attggctcga
    12781 gcagttcgcc cgagtgccgg tcgagctcga tatcgcctcc gaattccgct accgccagcc
    12841 ggtgctggag gagggcggcc ttgcgctctt catctcccaa tcgggcgaga ccgccgacac
    12901 gctggccgcg ctccgccacg cccgcgccca gggccagcgc atcgccgtcg tcgtcaacgt
    12961 gccgaccagc tcgatggcgc gcgaggccga ccttctgctc cccacccatg ccggacccga
    13021 gatcggcgtc gcctcgacca aggccttcac ctgccagctt gccgtcctcg ccgcgctcgc
    13081 cgccaaggtc gcgcgcgaca agggccggat gaccgaggag gaggagcgcg agatcgtcgg
    13141 ccacctcacc gaagcgccgg cggcgatgaa cgcggcgctg aaccatgacg aggaaatcga
    13201 ggcgatggcg ccggtcatcg cgcgcgcccg cgacgtgctc tatctcggcc gcggccccga
    13261 ttatccgatg gcgctcgaag gcgcgctcaa gttgaaggag atcagctaca tccacgccga
    13321 aggctatgcc gccggcgaga tgaagcacgg gccgatcgcg ctgatcgacg acgacgtgcc
    13381 agtgatcgtc atcgccccgt ccggcccgct gttcgagaag accgtctcca acatgcagga
    13441 ggtccaggcg cgcggcggca aggtcgtgct catctccgac gccgaaggca tcgccgcggc
    13501 cggcgagggc accatcgcca ccatcgagat gccgaaggtg catccgctga tcgcgccgat
    13561 ggtctacgcc atccccgtcc agttgctcgc ctatcatgtc gcggtggtga agggcaccga
    13621 cgtcgaccag ccgcggaacc tcgcgaagag cgtcaccgtc gaataagcga cgctgctcgc
    13681 cgaggatgag cttagccgat gggattgagg tagtaggccc gccgcgagct atcggcgaaa
    13741 tcgacatatt tgagcggcgc ccgctccagc tccaccggct gcggcaggcg ggtggcgaga
    13801 taggccttgg cgcgcgcctc gtcgtagctg acgtcgccgg aatagaccgc ctgcttcggc
    13861 atcaccagac cgagcgtgaa gaagcccttg aggaagtcgc cggcgacttg cccggcattg
    13921 gggaatttcc acttgccgag gtgaaacgaa taatcgccga catataaggc acgcccgggc
    13981 ttcacctcga acatcagggt gccgcgcgcg tagctgttgg agctgccgcc gctattcgga
    14041 ttggtcgcct gcgcggcgac atagatgccg ggctcggcga gcaccgccca gccctgccag
    14101 ttctggcgat cggcgttcga ccacaggccg gcgccgcggc cgaccttgaa aggcttgccc
    14161 ggaagatcgc gcctgcggct ctcgatcgtc ttggtggcgg gatcgtaggg cacgaacagc
    14221 agatgcggct ccggcacctt cggccccgga gcgatgcgcg tgtagaccac caccagcccc
    14281 tgccgggatt gcgccccgaa cacgagcggc ggcgtcggcg gcgcggcgcc gacaagcagg
    14341 gcgacggcgg ccgcgaggag catgattttc ttcatgcctg ggccatacac gaaaggcctg
    14401 ccaacctcaa ctgtttgaag ttattggcgt tttgcttccc cgctccgtca gccgatctgg
    14461 cggcggattc cttcgggaag ctcggccggg gcgcagagcc gtgcttcgac atcgtcctgg
    14521 accgagatgc ggatccgggt ccggcccagc acttcgatgc gggtgaaggc cggcgccatt
    14581 tccggctcgt cggaattgga cggcatttca aacgattcca tcgattcgag cgtgtagacg
    14641 cgcccttcct gccggaccga gcgcggcgtc tcggcgaagc tctgcgtcgc gtccgcatag
    14701 agccagcgca gccgcgcgcc gtcataatgg agggcgttga tcggatcgcc gcaactctcg
    14761 ccgacattga cgtagcttcc ggtgtcgatc gggatcgtga ccgccgcggg cttggcatcg
    14821 gccttggccg ccggcggcgc ctccgcaagg gccggggcgg cggccagcag tgcagcggcc
    14881 gctatcgtca atgtcttcat ccggtccccc tttgcggcgc ggcgaatcgc gcgccgccgg
    14941 agcttaggcg caaacggcgt ccgggggtaa tcgggcaaag agaagggcgc cgaagcgccc
    15001 ttctcctgtt ggccgtggca gcgcggggcc gcctagagca cgaagtcgct ggcggccagc
    15061 tcgatcgggc ccgacacctg gatgaagaag tcggccatgc tgtcgccgtc gcggtcggct
    15121 tcgaccatca cgccgaggcc gttgctctcg tagcgcagcg ctccggccgt gccggagaag
    15181 gcgccgtcgc cgatgaagct gaaggcgtcg ttgccggcgc cggtcgccgc atcgacgcgc
    15241 gagaggtcca tgacgtcgcc ctcctcgcgg ctgaagccct cgatcgtgtc ggccagcgac
    15301 ggcgcggtgc cggcatggcc gtcgaggaag cgatattggt cggcgccggc atcgcccgcg
    15361 agcaggtcgg tgccgagccc gccggtcagg atgtcgtcgc cggcgccggc gaacagccgg
    15421 tcgttgccgg agccgccgac cagcacgtcc atgccgctgc cgccgtcgat gacgtcgttg
    15481 ccggcaaagc cggagagccg gtcctggcca tcgaagcctt cgagatagtc gccgccggcg
    15541 ccgccatcga ggaagtcgct gccgacgccg ccgtaaagct cgtcatagcc cgcgccgccc
    15601 gacagcaggt cgtcgccgcc gtcgccatag aggcggtcgc tgtcctcgcc gccgtcgacg
    15661 acgtcgttgc cctcgccggc catgaccgtg tccgcgcccg cgccgccgaa gatcgtgtcg
    15721 gcgccgatgc cgccggcgat gtcgtccgcg ccgaggccgc cgtcgatcca gtcgtcgcca
    15781 tcctcgccgt cgagctcgtc gtcgccggcg ccgccgtcga gatggtcgtg gtcggaaccg
    15841 ccgctgaggc tgtcgtcgcc ggagccgccg tccatatggt cgttgccggc atcgccggcc
    15901 atccggtcgt ggccgcgccc gccgagcatg gtgtcgtcgt cgccgccgcc atgaagctcg
    15961 tcggcctggt cgccgccgct catataatcg gcgccggctt cgccgaccag gaagtccatg
    16021 ccggcgccgc catacatgaa atcgccgccg agaaggccga acagccggtc ggcgccggcc
    16081 tcgccataca tatggtcgga ctcgaggctg ccgccgagga aatcgtcgcc gtcgccgccc
    16141 cacatcgtat cgccatgctc gccggcgtta agcgcgtcgt cgccgccgag cccaaagagc
    16201 tggtcatggc cggcaatgcc gtgaatgtcg tcgttgagcg cgctgccgcg gatgatctca
    16261 ttcgcctcgg tgccgcgata catgcgttga tagaaactca ctattccctc ccaatggccg
    16321 agcaaaacgc gaccgttttc cttgcgaatc aatccattgc gctaatcatc tgttatgcat
    16381 gggactatca atgtggataa cgggagggcg ggaagcttat tcccggggcg gcgaaaaaac
    16441 tttgcgtgag ccgggccagg ccatgatcgg gtgccgatag ctgccggcgc gttaggcgga
    16501 aagcgacaga ccctgcgctt cgaggagggc ggtgcaccgc ccaacaaaaa agggcgccgg
    16561 agcgcccttt ttcgagtttg gcgtcgagcc ttacttgctc ttgttggact tgcgcatctg
    16621 cttgctgagc gcctgcgtca ccgcggcttc ggagccgctg gcggcgtcgc agctgaccgg
    16681 gccgttcttc ttctgcgtgg cgagcgcgtt gaccgcgacg ccgcagatgt tggcggcgac
    16741 ggagaccggc acctggacgt tgaccgggac ctggctgact tcgacgctga ggttgttggc
    16801 gatattctcg agattgaggt cgctgatgtt gacgttgacc aggccggcct ggttctgggc
    16861 cgcggcgggg gccgtcatcg ccatcgtcga aatcgcggcc gtggccgtca aaatg
//
LOCUS       DASVRK010000281        21306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_159074, whole genome shotgun sequence.
ACCESSION   DASVRK010000281 DASVRK010000000
VERSION     DASVRK010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 21306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21306
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159074"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VF589_11375"
     CDS             complement(<1..229)
                     /locus_tag="VF589_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8258224.1"
                     /translation="MYHPDLIRHPDSCPALVLNADYTPLSYYPLSLWPWQTAVKAVFL
                     ERVDIVANYAREVHSPTRTMHLPSVIALRQYV"
     gene            416..1294
                     /gene="gluQRS"
                     /locus_tag="VF589_11380"
     CDS             416..1294
                     /gene="gluQRS"
                     /locus_tag="VF589_11380"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX8258225.1"
                     /translation="MKNVVTRFAPSPTGRLHLGHAYSALLAHRFARDRGGSFLLRIED
                     IDRNRARAAFVDGIIGDLLWLGIELDGEIVYQSERLPLYAEALDRLSERGLVYPCFCT
                     RSAIAAEIAASVAAPHGPDGPVYRGRCRVLSEDERAQRRRTEPHAWRLDMVAALDSLP
                     AGASLRWFDEGREIPADPLAFGDVVLARKDAPTSYHLAVTVDDAAQDVSDVVRGRDLF
                     AATHVHRLLQALLGLPTPQYHHHALLTDAEGRRLAKRNGAPTLAELRANGADPHGLTD
                     DLLAGRLPAGYLAARS"
     gene            1304..1507
                     /locus_tag="VF589_11385"
     CDS             1304..1507
                     /locus_tag="VF589_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIG1 domain-containing protein"
                     /protein_id="HEX8258226.1"
                     /translation="MQTLLIILLVFFAGATLFVLAKGVIGMAQGKDVSGVRSQNLMRK
                     RVMYQAIAIVLVVLLILLASGRT"
     gene            1510..2085
                     /locus_tag="VF589_11390"
     CDS             1510..2085
                     /locus_tag="VF589_11390"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX8258227.1"
                     /translation="MVRLNQIYTRTGDAGETGLVDGSRISKAHPRAGAIGDVDEANSA
                     IGIALLHISDPRQQAMLRRIQNELFDLGADLATPGDDFTPGEMTLRVTAGQVDRLERE
                     IDAMNETLEPLRSFILPGGGAGAAHLHLARAIVRRAERSAVAAAAQVSLNPQALIYIN
                     RLSDHLFVLARLAASAAGGDLLWEPGATRGT"
     gene            2150..3394
                     /gene="egtB"
                     /locus_tag="VF589_11395"
     CDS             2150..3394
                     /gene="egtB"
                     /locus_tag="VF589_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX8258228.1"
                     /translation="MATQTRSVPADPLTARFEAVRALSSAIAAPLSDADATLQPMPDA
                     SPAKWHLAHTTWFFETFVLRDHVPGYALHDERWPFLFNSYYDGEGERHARARRGMLSR
                     PSLAEVLAYRAHVDAAMAAAIPALGPDALALVELGLNHEQQHQELMLIDLLAAFAENP
                     LLPALWPGEAPPLPVPPPLGWIEGATGRVEIGHDGAGFAFDCEGPRHEVILRPHALAD
                     RLTTNAEWLSFIDDGGYKRPELWLADGWAWVSEQRAAAPLYWRCGEGGWRRFGLDGLK
                     PVNLAEPVCHINYYEADAYARWAGARLPTEAEWETAAAARDPAEGNQLDAAGPVRPQG
                     GEASFGDVWQWTISAYLPHPGFRPAAGTVGEYNGKFMCGQFVLKGASCATPRGHSRAS
                     YRNFFYPHQRWMFAGLRLAKDL"
     gene            3396..4352
                     /gene="egtD"
                     /locus_tag="VF589_11400"
     CDS             3396..4352
                     /gene="egtD"
                     /locus_tag="VF589_11400"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX8258229.1"
                     /translation="MDVPRKIDTAFRDDVLAGLAAPIPAVPARWLYDRRGSELFEEIT
                     QLPEYYPTRVERGILETTAPEVARIVGPGSAVVEFGSGSSAKTPLLLNAVRPAAYVPI
                     DISGEFLRDSAAALQERFPGLAVHPVEADFTHPIELPAEARALPKLGFFPGSTIGNMV
                     ARTAVDLLRAMKETLGVGAWLLIGMDRIKEADVLLRAYDDAQGVTAAFNLNLLHRINA
                     ELDGTIPVEAFRHRAIWNDPMSRIEMHLEALRDVSFTVSGRAFAFARGATIHTENSHK
                     YGFRDSRLLLRAAGWGVCREWTDPDDQFVLILAEAEAPRFAP"
     gene            complement(4383..5312)
                     /locus_tag="VF589_11405"
     CDS             complement(4383..5312)
                     /locus_tag="VF589_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8258230.1"
                     /translation="MNRSVTSVALILSTILAAPALAQATRTWVSGVGDDVNPCSRTAP
                     CKTFAGAISKTAAGGEINVIDPGGFGAVTITKSLTIDGHGTQASILSTGTTGINVNGA
                     GIVVNLRNLTINGGNSTVGSGIRIINADSVNVDNVTIFNYAGTGTVGRGVSIESSTPN
                     ARVSITNSIFYNLGNVGIAAFPAVGPLLLDVDNVRIFRGADHGIHLGNNTKLTLSRSI
                     VSGHPLGAGVALNLAGASAHISDSVLSNNRFGISSGAGGGTPTTRLFGTVITGNTLDG
                     LRITAGSVFSHGNNAIVGNAGNETPTPPILGTQ"
     gene            complement(5535..5798)
                     /locus_tag="VF589_11410"
     CDS             complement(5535..5798)
                     /locus_tag="VF589_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258231.1"
                     /translation="MNGEDDREPAGPDGALFRAALGLVAMIGGLLGLAGLYFVPIPEG
                     NREPLLLALGLVLGWGSTVVSYEFGSSPAGRKAADAGVRQTPS"
     gene            complement(5927..6466)
                     /locus_tag="VF589_11415"
     CDS             complement(5927..6466)
                     /locus_tag="VF589_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HEX8258232.1"
                     /translation="MDVTTLIEEAIGREGGYVNHPADTGGATRWGVTEAVARAHGYRG
                     NMRFYPREEAVAVYRRIYWLRPGFDRVAALAPRLAAEMFDTGINMGPDVAAGFLQRAL
                     NALNRGGSDYPDLLVDRRIGDLTIAALAAFLALRGAPGETVLLKALEALQGERYVSLA
                     EQRPANEAFLYGWLANRIG"
     gene            6581..9136
                     /locus_tag="VF589_11420"
     CDS             6581..9136
                     /locus_tag="VF589_11420"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX8258233.1"
                     /translation="MGLTLILLLAVGAYLLFELRRLGRKVDELERDLAELRHWREAPQ
                     PEAATAPAAAPALPASEPASRASRAAEPAPELDDALPEPRPPAAAEPVRETAAALFER
                     FVGGRLLIWTGGVALAVAGVFLVRYSIEIGLVTPPVRMLIAAGFGLLLIAAGEYARRR
                     SGDDQRIAQALVGAGIFVLYAATYGSLTLYGLIGLGTASALMALITAGALALALRHGV
                     PTAAMGLTGGFLTPLLVGDPDASAVPLLGYLALLNAALFALAQRRGWSWMAVAAVVLS
                     FLWTAALLRSDANDALAAGIFVAAMGIAASLAEPGGGRPLRLIQPAALALIQLAVLVG
                     RTDLGLPAWALFGALAAASLFLAERRAEYRLLPAIALALALVLLAGKAALGDPLIAYA
                     GAVATLLFAGFGWWRLKRGAEPMLWAGLACAGLAGPAIILRAGEAELLARPLWGALFA
                     ALAAAAGFLAWSRRGDAAGASAAVPALASATAALLLMIAAYDLVPLEWVPAAWLALAA
                     GAATWAARLGAKEPAPVAGVVAGAGAILEVALLPALWLIIALSLAGEAARVDRLPSPL
                     DALRLLLIPAALLVAIRHLAPAWPERARAALTLGAVLFGTLGAYVLFKQLLALRDAED
                     FVARGFAERTLITQALFAAGWLLVARAAGRAGWRRLGIAFTALAAARLVWFDLIIHNP
                     ALTSQWVGSLPVLNLLLPAYLGAAAWLFAARLRAGAASGPWLSLSLLSLLAGSLLLVR
                     QGFQGPLLAEADLPRGEFYGYSLAGLLLAIALLGAGVRRADKPLRFAGLILLTATVFK
                     VFLIDAAELEGVLRILSFLGLGVALIGVGKLYATVLSSERDGKAGAEAAPRPG"
     gene            complement(9133..9270)
                     /locus_tag="VF589_11425"
     CDS             complement(9133..9270)
                     /locus_tag="VF589_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258234.1"
                     /translation="MAGQLRYEIVGADAFVHGDTDGDGLADLLIRVNGVASLAAVDFV
                     L"
     gene            9410..9559
                     /locus_tag="VF589_11430"
     CDS             9410..9559
                     /locus_tag="VF589_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258235.1"
                     /translation="MGIGDIAARLGVSRNMVRTHVAVLRDKLGVNSTLAVATQIRRAA
                     GPLLR"
     gene            9881..10300
                     /locus_tag="VF589_11435"
     CDS             9881..10300
                     /locus_tag="VF589_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258236.1"
                     /translation="MGIILNRLFARHCLVACALLVCVAARAPTSFPEYLEQMIKYHRV
                     TVGLEIASRCGLLNEAERASYERDYAKASDVVVRLMAENFEREEALDSLEILQSASAA
                     GSRQEFPRCGTEAAEWMETSKREIKELVAYADQYSKH"
     gene            complement(11112..11414)
                     /locus_tag="VF589_11440"
     CDS             complement(11112..11414)
                     /locus_tag="VF589_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258237.1"
                     /translation="MKPDSDIALERVQSETLGALLSIQSYREVDAGYFERLRSAVTDA
                     VAFFSEEERVPPALLSELTQSANILRNEATAFPGRTAACLEMADWLEAQERVLARD"
     gene            complement(11554..12468)
                     /locus_tag="VF589_11445"
     CDS             complement(11554..12468)
                     /locus_tag="VF589_11445"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEX8258238.1"
                     /translation="MQLAKRERGIPDQLRQLALVLGFGAIMGLAGPFGTYPALDRPVR
                     YAFWIGLVLAGYAFALGAARLSELFPGLARAGRVTMNGLIALASAVPMTFVVAWTLSL
                     VHPGRVVPPIQLPVLFACVAAVQLVIIVALIRPVVAPLRPAQPLHPMAAPNAPDAGAP
                     GHTAALHQPEMPENKAPIAPAERAADARESEAPSVAPPAFPAVLLARMPRGLGPEIVA
                     LEAEDHYVRVHTPRGSTLVLMRLSDAVELLDAAIGLRVHRSWWVARQAIASFDRDGQQ
                     LTLRLTNGAQVPVGRTYLAAVRTALRDT"
     gene            12648..13784
                     /locus_tag="VF589_11450"
     CDS             12648..13784
                     /locus_tag="VF589_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8258239.1"
                     /translation="MEFFLIVFLPIAFLTLVVGGAAAFTWRCARSAGAIWMFWAPAAV
                     IHAGWWLLPELDEGVAAIVRWFAIVWIGAILAGAILFVPFAAIKVAGRLSGRGEADDG
                     VMITTYGTLALALGLIASVGSTSEARIREEIVAIRDLPAGLDGVRIANLADVHIGRFV
                     TVADLGRAIRRIDARGADYLVVTGDLIDDFSQLDRTLGALETSKALPVLSILGNHERM
                     GDIEATLAAYGRRRRTRLLINDLVDLEHEGAAIRFVGIDYPMAEDGGHVASPARQDRL
                     LRDFAGRAFARGQGRGLTVALSHHPGFFPFAAARGARLTLAGHTHGGQVALSGRPIVE
                     AYRYMAGRYRIGEAHLDVSRGFGHWLPLRIGVPRETVILTLVRA"
     gene            complement(13812..13888)
                     /locus_tag="VF589_11455"
     tRNA            complement(13812..13888)
                     /locus_tag="VF589_11455"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13852..13854),aa:Pro,seq:ggg)
     gene            14016..14489
                     /locus_tag="VF589_11460"
     CDS             14016..14489
                     /locus_tag="VF589_11460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8258240.1"
                     /translation="MEIHYGLATLEETRGLSGIEALRAMIEGRLPAPPIAKAMGFRLV
                     EVGEGTAVFEGEPGPHLLNPLGGVHGGFALTLIDSAAGCALHAALEAGVGYATVETKT
                     NMTRPIAPDGGMVRAEGRLLTRGRQIATAEARILDREGRILAHGTSTLIILAPRG"
     gene            14729..15064
                     /gene="yajC"
                     /locus_tag="VF589_11465"
     CDS             14729..15064
                     /gene="yajC"
                     /locus_tag="VF589_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX8258241.1"
                     /translation="MFASPAYAATGAAASGGATGFFVSVFPLVLIFVIFYFLLIRPQQ
                     RRMKQHQAMIAAVKKNDVVVTGGGLVGKVTRVDEHEVEVEVAPNVRVKAVKGTLSEVR
                     PHGAKPAND"
     gene            15064..16665
                     /gene="secD"
                     /locus_tag="VF589_11470"
     CDS             15064..16665
                     /gene="secD"
                     /locus_tag="VF589_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX8258242.1"
                     /translation="MLDFPRWKVWAVSLLMLVGIALAVPSFFPQSAVARWPGLLPSAQ
                     INLGLDLAGGSHLLLQADTSGVARQRLEQMEETLRLEMRQAEPRIEIGEISTTGGRVS
                     FLVRNPAQVDAAREIVYNQTQPAGLTGQRDWNVNVIDTSRIIVEPTSAGLASALESAM
                     NTAREVVYNRVDPEGTREVTVVRQGGDRILVQVPGLQDPEGLKALLGKTARLDFKLVD
                     LSADPALVAQGRSPAGSQTLPYPDNPSGAPGIAVQRRAMVTGDRLVDATQGFDPQTNE
                     AVVNIRFDGAGGKRFAKVTQENVGKPFAIILDNVVISAPNINEPILGGQAMISGSFTV
                     ETANELAVQLRSGKLPVDLKVVEERTVGPDLGADSIRLGVIAGTVGTLAVLVFMILTY
                     GRFGVYADIALVLNVVLILGIMAIFNATLTLPGIAGFVLTIGAAVDANVIINERIREE
                     QNRGRNVLQAIEHGYKEAQTAIFDANITNVIAGILLFYFGTGPVRGFAVVMMIGIVTS
                     VFTAVSVTRMFVSIWLRKRPTELRI"
     gene            16715..17680
                     /gene="secF"
                     /locus_tag="VF589_11475"
     CDS             16715..17680
                     /gene="secF"
                     /locus_tag="VF589_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX8258243.1"
                     /translation="MRLMKFVPENTNIDFLRWRTVAISISVLLMAASIALIAVKGLNF
                     GVDFAGGQLIRVSFAQNVSLDELRERVTNVGLGEPSIQEFGSAKEVAIRLPLPEGGEE
                     GANKAASKLRKMLVQQYPGARIDAVETVSGRVSGELVRSGALALLLAMIGIAIYIWIR
                     FEWQFGVGGLFSLLHDVTLTLGLFSLTQMEFDLNVVAALLTLIGYSLNDTIVIYDRIR
                     ENLRKYRKMDITSLLNLSMNETLARTVATNLSMLLALGSLMFLGPEVIQSFTTALLFS
                     VVVGTYSTVYIASPFLIWLKVNSDSFVPKAPASGSAERVSHNEGY"
     gene            17680..18057
                     /locus_tag="VF589_11480"
     CDS             17680..18057
                     /locus_tag="VF589_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX8258244.1"
                     /translation="MPRWDRDAAASGPVIRGFTDRGFAIDGGVYQGLFLTPASAHEWR
                     PPALADLNEKHLEPVLHIDPAPEFLLLGTGAALAFPPRALIKALDKRGIGLEVMDSRA
                     AARTWGMLRSEERWIVAAIMPLA"
     gene            complement(18041..19231)
                     /locus_tag="VF589_11485"
     CDS             complement(18041..19231)
                     /locus_tag="VF589_11485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8258245.1"
                     /translation="MLRVLTLSTLFPNAAQPTFGIFVERQTLGLAALPGVELQVVSPV
                     GLPPWPLSRHPHYAERARLPLRETWKGLVVHRPRFGVVPRLGEARTARSMAAALLPLL
                     RDLRADLPFDVIDAQFFWPDGPAAKILSDALGVPFSVKARGGDINHWGTRPAVAPQLV
                     EAGRAAGGLLAVSGALAGVMASLGLPDGKIKVHYTGVDFDRFIPVARAAAKAALGVEG
                     PLIVSAGALIPRKGQRLAIEALRQIPGATLLIVGQGPERRRLEAHAAAAGVADRVRFL
                     GNRPHEELPGLFAAADVMVLPSASEGLANVWVEALACGTPIVIGDVGGAREVLTRPEA
                     GRLPPLEPEAIAAAVRELLADPPAQAEVRKVVERFSWDTNSRTLYDHLAGVAAAGRAL
                     RPAA"
     gene            19354..20175
                     /locus_tag="VF589_11490"
     CDS             19354..20175
                     /locus_tag="VF589_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX8258246.1"
                     /translation="MLPKNYRPLALLLAAAATLPLAGCAGRGAREGADTAYVARDVNT
                     LYTAAKGRLDRGNYKQAAALFDEVERQHPYSVWARRAQLMSAFSYYLAKDHTKSIESA
                     RRFLSIHPGNKDAPYALYLVALNYYEQIEDVNRDQSISKQALEALGELVRRHPTTRFA
                     ADARLKVDLVRDHLGGKEMEIGRFYQRRKQWLASITRFRTVIDEYQTTTHTPEALMRL
                     TESYLALGITDEARKSAAVLGANHPGSKWYQRAYQLVQRHGAPPAAAAPAAAPAA"
     gene            20239..>21306
                     /locus_tag="VF589_11495"
     CDS             20239..>21306
                     /locus_tag="VF589_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8258247.1"
                     /translation="MLTSLSIRDVVLIETLDLEFGRGLGVLTGETGAGKSILLDALGL
                     ALGARADSLMVRAGQAQAVVSVSFDIPRAHPALGLLDENGLGGEPGEPLVIRRILKAD
                     GGSRAFVNDQSASVALLRELGPMLVEIHGQHDDRGLLNPRGHRALLDSFGGIDRAPCD
                     AAFRAWRSAEARLAAAREQLEIAARDREWLDHAVAELVKLAPRAGEEAELADARAQMQ
                     KGARLAEDIALVAQTLDGSDGGLALLRQAARRLERVAGEDERLGEALAALDRAVNEAC
                     EAEDRLGEAAEAMAFDPARLEESETRLFELRAIARKHRVDPEALPALAEELAERLVGI
                     EAGDAGLAAMEKGLRASRLAYE"
BASE COUNT         3232 a         7162 c         7415 g         3497 t
ORIGIN      
        1 tcacatattg ccggagcgcg atcaccgaag gcaggtgcat ggtccgcgtg gggctgtgca
       61 cctcgcgggc gtaattggcg acgatgtcga cgcgctccag gaacaccgcc ttgaccgcgg
      121 tttgccaagg ccagaggctg agcggataat aactgagcgg cgtataatcg gcgttcagca
      181 ccagcgcggg acaggagtcc ggatgtcgga tcaggtcagg atggtacatg aaccggcaca
      241 cgcctcccgt ttcatggagc tgaactcccg aaacgccgcc cccggacctg tcgcacgcag
      301 aacgcgggag aattcggctt tgcggcctca tctcctgttc gaccacgctt ttgctgccga
      361 agcgtgacgc cgtcatgaca ccacaactcg cgactcgcgg tcaagggccg cattcatgaa
      421 aaacgtcgtc acccgcttcg ctccgtcgcc gaccgggcgg ctgcatttag ggcatgccta
      481 ttcggccctg ctcgcgcacc gcttcgcgcg cgatcgcggc ggtagcttcc tgctccggat
      541 cgaggatatc gaccgcaacc gcgcccgcgc cgccttcgtc gacggcatca tcggggacct
      601 gctctggctc gggatcgagc tggatgggga gatcgtctat cagtccgaac gcctgccgct
      661 ctatgccgag gcgctcgatc ggctgagcga gagggggctg gtctatccct gcttttgcac
      721 ccgctcggcc atcgccgccg agatcgccgc cagcgtcgct gccccgcacg ggccggacgg
      781 gccggtctat cgcggccgct gccgcgtcct cagcgaggac gagcgcgccc agcgccgccg
      841 gaccgagccg cacgcgtggc ggctcgacat ggtcgctgcg ctggacagcc ttcccgcggg
      901 cgcgagcctc cgctggttcg acgagggccg cgaaatcccg gccgatccgc tcgccttcgg
      961 cgacgtcgtg ctcgcccgca aggacgcgcc gaccagttac catctcgccg tcacggtcga
     1021 tgacgccgcc caggacgtca gcgacgtggt ccgcggccgc gacctgttcg ccgccaccca
     1081 cgtccaccgc ttgctccagg ccctgctcgg cctgccgacg ccgcagtacc atcatcatgc
     1141 cctgctcacc gacgccgagg ggcgccggct ggccaagcgc aacggcgcgc caacccttgc
     1201 cgagctccgc gcgaacgggg ccgatccgca tgggctgacc gacgatctgc tcgccggacg
     1261 ccttcccgct ggatatttgg ccgcccgttc ctagataggg gccatgcaaa ccctcctcat
     1321 catcctcctc gtgtttttcg ctggcgccac tttgttcgtg ctggccaagg gggtgatcgg
     1381 catggcccag ggcaaggacg tcagcggcgt ccgctcgcag aacctgatgc gcaagcgggt
     1441 gatgtaccag gcgatcgcga tcgtgttggt cgtgctgctg atcctgctcg ccagcggcag
     1501 gacctgacgc tggtccggct caaccaaatc tacacccgca ccggcgatgc cggcgagact
     1561 ggactggtcg atggctcgcg catctcgaag gcgcatccac gcgccggcgc catcggggat
     1621 gtcgacgaag ccaatagcgc catcggcata gcgctcctcc acatctccga cccccgccag
     1681 caggcgatgc tgcgccgcat ccagaacgaa ttgttcgatc tcggcgccga tctcgccacc
     1741 cccggcgacg atttcacgcc cggcgagatg acgctccgcg tgaccgccgg gcaggtcgac
     1801 cgcctcgagc gcgagatcga cgccatgaac gagacgctgg agccgctccg cagcttcatt
     1861 cttcccggcg gcggcgcggg cgcggcccat ctccacctgg ctcgcgcgat cgtccgccgc
     1921 gccgagcgca gtgccgtcgc cgccgccgcg caagtgagcc tcaacccgca ggcgctcatc
     1981 tacatcaatc gtctgtccga ccacctgttc gtcctcgccc ggctcgccgc ctcggccgcg
     2041 ggcggagacc tgctttggga accgggcgct acccgcggga cttaacctcg gttagggaaa
     2101 tttggcgctg gggccgtgct cctgtaatgg cgcggtgggg agaagcagca tggcaacgca
     2161 gaccaggtcc gttccggcag atccgctgac cgctcggttc gaagccgtcc gcgccctcag
     2221 ctccgcgatc gccgcgccgc tgtccgacgc cgacgcgacc ctgcagccga tgcccgatgc
     2281 ctcgccggcc aaatggcatc tcgcccacac gacctggttc ttcgagacct tcgtcctgcg
     2341 cgaccatgtt cccggctatg cgctccacga cgagcgctgg ccgtttctgt tcaacagtta
     2401 ttatgatggc gagggcgagc gccacgcccg ggcgcggcgc gggatgctca gccgcccgtc
     2461 cctggcggaa gtcctcgcct atcgcgccca tgtcgatgcg gccatggcgg cggcgatacc
     2521 ggcgctgggg ccggacgcgc tggcgctggt cgagctcggg ctcaatcacg agcagcagca
     2581 tcaggagctg atgctgatcg atctgctcgc cgcctttgcg gagaatccgc tgctgccggc
     2641 gctgtggccg ggcgaggcgc cgcccttgcc ggtgccgccc cccctggggt ggatcgaagg
     2701 cgcgacgggg cgcgtcgaga tcggccatga cggcgcgggc ttcgccttcg actgcgaggg
     2761 gccgcggcac gaggtgatcc tccgcccgca cgcgctcgcc gaccggctga cgaccaacgc
     2821 cgagtggttg agcttcatcg acgatggcgg ctataagcgg ccggagctgt ggctggccga
     2881 tggctgggcc tgggtctcgg agcagcgcgc cgcggctccg ctctactggc gctgcggcga
     2941 gggcgggtgg aggcgcttcg gcctcgatgg cttgaagccg gtcaacctcg ccgagccggt
     3001 gtgccacatt aattattacg aggccgacgc ttacgcccgc tgggccgggg cgcggctgcc
     3061 gaccgaggcc gaatgggaga ccgccgccgc cgctcgcgac cccgccgaag gcaaccagct
     3121 cgacgccgcc ggcccggtcc gtccccaggg cggcgaagct tcgttcggcg acgtctggca
     3181 atggacgata agcgcctatc tgccgcatcc gggcttcagg ccggcggcag ggacggtcgg
     3241 cgaatataac ggcaagttca tgtgcggaca gttcgtgctc aagggcgcga gctgtgccac
     3301 tccccgcggc cacagccggg ccagctaccg caacttcttc tacccgcacc agcgctggat
     3361 gttcgccggg ctgcggctgg ccaaggacct ttagcatgga tgtgccgcgc aagatcgaca
     3421 ccgcttttcg cgacgacgtg ctcgccggcc tcgccgctcc gatcccggcc gtgccggcgc
     3481 ggtggctcta cgaccggcgc ggatcggagc tgttcgagga gatcacccag cttcccgaat
     3541 attatccgac ccgggtagag cgcggcatcc tcgaaaccac cgcgcccgag gtggcgcgga
     3601 tcgtcggacc cggcagcgcc gtcgtcgaat tcggttcggg gtcgtcggcc aagacgccgc
     3661 tcctactcaa cgccgtccgc cccgccgcct atgtgccgat cgacatatcc ggcgagttcc
     3721 tgcgcgattc ggcggccgcg ctgcaggagc gcttccccgg cctcgccgtc catccggtcg
     3781 aggccgattt cacccatccg atcgagcttc cggccgaggc gcgggcgctg ccgaagctcg
     3841 gcttcttccc cggctcgacg atcggcaaca tggtcgcccg caccgccgtc gacctgctcc
     3901 gcgcgatgaa ggagacgctg ggggtcggcg cgtggctgct gatcggcatg gaccggatca
     3961 aggaagcgga cgtgctgctc cgggcctatg acgatgccca gggcgtcacg gccgcgttca
     4021 acctcaacct ccttcatcgc atcaacgccg agctggacgg caccatcccg gtcgaggcgt
     4081 tccgccaccg cgcgatctgg aacgacccga tgagccggat cgagatgcac ctggaagcgc
     4141 ttcgcgacgt ctccttcacc gtctccgggc gcgccttcgc cttcgccagg ggcgcgacca
     4201 tccataccga gaacagtcac aaatacggct tccgcgactc ccgccttctg ctccgcgccg
     4261 ccggctgggg cgtctgccgg gaatggaccg acccggacga ccagttcgtc ctcatcctcg
     4321 ccgaagccga agcgccaagg ttcgcgccct aggtgcgaac ccgggcgcgc ccccgccgcg
     4381 gcttattggg tgccgaggat cggcggagtc ggggtttcgt tgccggcatt gccgacgatg
     4441 gcgttgttgc cgtgcgagaa gacgcttccc gcagtgatgc gcaggccgtc gagggtgttg
     4501 ccggtgatga cggtaccgaa cagacgcgtg gtcggagtgc cgccccccgc gccgctggag
     4561 atgccgaacc ggttgttcga aagcacgctg tcgctgatgt gcgccgatgc gcccgccagg
     4621 ttgagggcca ctccggcgcc gagcggatgg ccggagacga tcgagcgcga cagggtcagc
     4681 ttggtgttgt tgccgaggtg gataccatgg tccgcgcccc ggaagatcct gacattatcc
     4741 acgtcgagca gcagcggacc aacggcggga aaggcggcga tgccgacatt gccgagattg
     4801 tagaaaatgg agttggtgat cgagacgcgc gcgttggggg tgctgctttc gatcgagacg
     4861 ccgcggccga cggtgccggt gccggcatag ttgaagatgg tgacattatc gacgttcacc
     4921 gagtcggcgt tgatgatgcg gatgccgctg ccgacggtgc tgttgccgcc gttgatcgtg
     4981 agattgcgca agttgacgac gatgccggcg ccgttgacgt tgatgccggt ggtgccggtg
     5041 gagaggatcg aggcctgcgt cccgtggccg tcgatggtca gcgacttggt gatggtgacc
     5101 gctccgaagc cgccgggatc gatgacgttg atctcgccgc cggccgcggt cttggaaatg
     5161 gcgccggcga aggttttgca gggcgccgtg cggctgcacg ggttcacatc gtcgccgact
     5221 cccgagaccc aggtgcgggt cgcctgcgcg agcgcgggag cggcgagaat cgtcgacaga
     5281 atgagcgcga ccgaggtaac ggatctattc atcttgtttc ccccttcgtt ttgaacagga
     5341 aagccccggc ccccaccaaa gattgcccgg catcggccgg catttcttcc tgttcggtcc
     5401 aaacaacgta catctattcg cccgccccgt ccatgaaacg acgttgcggt gcgacctcat
     5461 gctccacttt tttgctccac atccgaccga tcgcggcgat tgttttgcgc tatcctcgcg
     5521 caaagccggg ctttttatga gggcgtttgc cggacgccgg cgtccgcggc ctttcgcccg
     5581 gccggcgacg atccgaattc gtagctgact acggtgctgc cccagccgag caccaggccg
     5641 agggcgagca gcaagggctc gcgattgcct tccggtatcg gcacgaaata gaggccggca
     5701 aggccgagca ggccgccgat catcgccacc aggccgagcg cggcgcggaa cagcgcgccg
     5761 tcagggccgg ctggctcccg gtcgtcctcg ccgttcacgc cggcctcccc acgtgctgca
     5821 ggttgcggtg gtccttcata aaaactccaa cgaaatccgt tgagcgggac gggcctggga
     5881 gtggggtgca cattatcttg tcattcttgt cactatgcgg ggtgtttcat ccgatccggt
     5941 tggctagcca tccgtagaga aaggcctcat tggcgggacg ctgctccgcg aggctgacgt
     6001 aacgctcgcc ctgaagcgct tcgagcgcct tcaggaggac cgtctcgccg ggggcgccac
     6061 gaagcgcgag gaaagctgcc agggcggcga tggtgaggtc accgatccgg cgatcgacca
     6121 ggaggtccgg ataatcgctt ccgccgcggt tgagcgcatt gagcgcgcgc tggaggaagc
     6181 cggcggcgac gtcaggaccc atgttgatgc cggtgtcgaa catttcggcg gcgaggcgcg
     6241 gcgccagggc cgcgacccgg tcgaagcccg ggcgcagcca gtagatgcgg cggtagaccg
     6301 ccaccgcctc ctcgcgcgga tagaagcgca tgttgccgcg atagccgtgc gcccgcgcta
     6361 ccgcctcggt gacgccccag cgggtcgccc ccccggtgtc ggccggatgg ttgacgtagc
     6421 cgccctcgcg gccgatcgct tcttcgatga gcgtggtgac atccatggcc gcgttcctcc
     6481 ttgccgagtc gtggaacata gatggaacaa actgctgtag gaaagcggtt tgagcggaaa
     6541 tagccatgac aggggcggcg gggggcccta gcttgcttcc gtgggattga cgctcatctt
     6601 gctgctggcc gtcggcgcct atttgctgtt cgagctgcgc cggctggggc ggaaggtgga
     6661 tgagctagaa cgcgacctgg ccgagcttcg gcattggcgc gaggcgccgc agcccgaggc
     6721 tgcgaccgct ccggccgcgg cgccggctct gccggcgagc gaaccggcga gtcgggcgag
     6781 cagggcggcg gagccggctc cggaactcga tgatgcgttg cccgagccgc gcccgccggc
     6841 tgccgccgag ccggtccggg agacagccgc ggcgctgttc gaacgcttcg tcggcggccg
     6901 gctgctgatc tggaccggag gcgtcgcgct ggcggtggcc ggcgtgttcc tggtccgtta
     6961 ctcgatcgag atcgggttgg tgacgccgcc ggtgcggatg ctgatcgccg ccgggttcgg
     7021 gctgctgctg atcgcggccg gcgaatatgc gcgccggcga agcggcgacg accagcgcat
     7081 cgcgcaggcg ctggtcggcg ccggcatctt cgtcctctac gcggccactt acgggtcgct
     7141 gacgctctac ggattgatcg gcctcgggac cgcctcggcg ctgatggcgc tgatcaccgc
     7201 gggggcgcta gcgctggcgc tgcggcatgg cgtgccgacc gctgccatgg gcctcaccgg
     7261 cgggttcctg acgccgttgc tggtcggcga ccccgatgcg agcgcggtgc ctttgctcgg
     7321 ctatctggct ttgctcaacg cggccctgtt cgcgctggcg cagcggcgcg gctggagttg
     7381 gatggcggtc gcggcggtgg tgctgagctt cctgtggacg gcggccttgc tccggtcgga
     7441 cgcgaacgac gcgctcgcgg ccggtatatt cgtggcggcg atgggcattg ccgcctcgct
     7501 tgccgagccc ggcggggggc ggccgctgcg gctgatccag ccggcggcgc tggcgctgat
     7561 ccagctcgcg gtgctggtgg ggcggacgga tctgggcctg ccggcctggg ccttgttcgg
     7621 agcgctcgcc gctgcgagcc tgttcctggc ggagcgccgg gcggaatatc ggctgctgcc
     7681 cgcgatcgcg ctggcgctgg cgctggtgct gctggccggc aaggcggcgc tgggcgaccc
     7741 gctgatcgct tatgccggcg cggtcgcgac cttgttgttc gcgggcttcg gctggtggcg
     7801 gctgaagcgc ggcgccgagc cgatgctctg ggccgggctc gcctgcgccg gcctcgccgg
     7861 gccggccatc atcctgcggg cgggcgaagc cgagttgctc gcgcggccgc tgtggggcgc
     7921 gctgttcgcg gccctcgccg ccgccgccgg attcctggcg tggagccggc gcggcgatgc
     7981 agcgggcgcg agcgcggccg tgccggcgct ggcctcggcg acggcggcgc tgctgctgat
     8041 gattgccgcc tacgatctgg tgccgctcga gtgggtgccg gcggcgtggc tggcgctggc
     8101 ggccggggcg gcgacctggg cggcgcggct cggcgcgaag gagccggcgc cggttgcggg
     8161 cgtcgtggcg ggcgccggcg cgatcctgga ggtcgcgctg ctgcccgcgc tctggctgat
     8221 catcgcgctg tcacttgcgg gagaagcagc gcgggtcgac cggcttccct cgccgctcga
     8281 cgcgctgcgg ctgctgctga tcccggcggc gttgctggtg gcgatccggc atctggcgcc
     8341 ggcgtggccg gagcgggcgc gcgccgcgct gacgctcggg gccgtcctgt tcgggacgct
     8401 cggcgcttat gtgctgttca agcagttgct cgcgcttcgc gacgcggagg atttcgtcgc
     8461 ccgcggcttc gccgagcgga cgctgatcac gcaggcgctg ttcgcggcgg gctggctgct
     8521 cgtcgcccgc gccgccggtc gcgcgggatg gcggcggctg ggcatcgcct tcaccgcgct
     8581 tgcggcggcg cggctggtct ggttcgacct catcatccac aaccccgctt tgacgagcca
     8641 gtgggtggga agcctgccgg tgctgaacct gctgctgccg gcctatcttg gcgcagccgc
     8701 ctggctgttc gccgcacgcc tccgcgccgg cgccgcgagc ggcccgtggc tgtcgctgtc
     8761 gctgctgtcg ctgctggccg gtagcctgct gctggtccgg cagggcttcc aaggcccctt
     8821 gctggccgag gccgatctcc cgcgcggcga attttacggc tattcgctag ccgggctgct
     8881 gctcgccatc gccttgcttg gcgcgggcgt caggcgggcc gacaagccgc ttcgcttcgc
     8941 cggtctgatc ctgctcaccg cgaccgtgtt caaggtgttc ctgatcgacg cggccgagct
     9001 cgagggcgtg ctgcgcatcc tctccttcct cggcctcggc gtcgcgctga tcggggtcgg
     9061 caagctttat gcgacggtgc tgagctccga gcgggatggc aaggcggggg ccgaagcggc
     9121 gccacggccc ggctaaagca cgaaatccac cgcggcgagg ctggcgacgc cgttcacgcg
     9181 gatgaggagg tcggccaggc cgtcgccgtc ggtatcgccg tgcacaaagg cgtcggcgcc
     9241 gacgatctcg tagcgcaatt gtccggccac gccgccgaag gccgcggtgc cgatgaaggc
     9301 gcaattctga tcgccggcgc cggccgcgtt ggcatcgacc cggcgaaggt ggagcttgtc
     9361 gccgccccct cacggctgaa gtcgccgact aggtcggcga gcggccggca tcgggatcgg
     9421 cgacatcgcg gcccggctcg gcgtgtcgcg caacatggtg cgcacccatg tcgcggtgct
     9481 gcgcgacaag ctcggcgtca acagcacgct cgccgtcgcc acccagatcc gccgcgccgc
     9541 cggcccgctg ctccgctagc cggtccacgt ggggccgttc atcccgccgg gctgacaacg
     9601 cacgagctgg cgcttgcgat catgcgcccc cgccagattg atgtcgccga tctagcggcc
     9661 gtaaagctca tccagcggcg cacggggcat agcctcaaac ggctagggga cgacgctatg
     9721 tcacgtcgga gaaggctaca gcgggcgagc tgctacgttg gcgcactaga cgacaggcca
     9781 aggccggcgg ctctgtcggc gggtggccga atggtacttc gagaagctca agtgccttga
     9841 gcagcaagct caatgagata ggagtgaaac gctctcgaag gtggggatta ttttgaaccg
     9901 gctttttgca aggcattgct tagtcgcctg tgcccttctg gtctgtgtcg cagctcgcgc
     9961 accgacgagc tttccagagt atcttgagca gatgatcaag taccaccgcg tgaccgtggg
    10021 cctcgagatt gccagccgat gtggtttgtt gaatgaagct gaaagggctt cgtacgagcg
    10081 ggattatgcg aaggccagcg acgttgtagt ccggctcatg gctgagaact tcgagcgtga
    10141 ggaggctctc gactcgcttg aaattttgca gtccgcttcc gctgcaggtt ctcgccagga
    10201 gtttccacga tgcggaaccg aggcagcaga atggatggaa acctccaaac gcgagatcaa
    10261 ggaattggtc gcttatgccg accaatattc caaacactag gaggcgcttc gctccgccag
    10321 cgcggtgcgt ggcggctgcc ttgcgggagc aacgggataa gggtcgggtc caatcggaag
    10381 acggacccgg cacctaccag cttcgtaagg tcattaagcc ttcgtaagct tcttcggctt
    10441 gaaagcggcg attgctgctg ctgcgttcgc ccagcgagct gcccttgcct ccacaacttc
    10501 gtccgcatcc agaagacgct tcgcgtcact cccgccatgg cagcgagcgt taccaatcga
    10561 tcttggaagg acagcgatta taagaagcaa aacagctgat atcacctcta ccggggagtc
    10621 aggaaaatgc gaaagatgca tcgcgtcggg tttctgctgc cgatgctact aggctgcgcg
    10681 ccaaatgcgg aaggtcccgc ggcgccacat gaacggcttg cagtttcgca accaaagcct
    10741 ccgcccgacc cgctgccgcc ctatcctccg ccgatgccgc acccgctagg cgccgtgttc
    10801 ttcgaacccg atagcgtcgt catgaaggga gagacctaca gccgcatcct ggaactcgta
    10861 cggcagggcc atcggcattg gcccgggctt acgctcaacg cctatgtcat caaagacgac
    10921 gccgctaacg agcggctggg ttgtgaaagg gcagcgcgcc tcaaggcaat gctgtcggag
    10981 gcaggcgttg agactatcga cattgtcctc agcaggttcg ctgagaaatc cgacttgggc
    11041 gtagttgaag tctggcccgt gcgcagcggc gatcggggat ggtgtccgca agccggagcg
    11101 aacattccgg atcagtcgcg agcgagcacc cgttcctgag cttcaagcca gtcggccatt
    11161 tcaaggcaag ctgcagttcg ccccgggaag gcggtcgcct cgttgcgcag gatgtttgcg
    11221 gattgtgtca gttcgctgag cagcgccgga ggcacgcgct cttcctcgga aaagaaggct
    11281 accgcgtctg tcaccgcgct cctgagccgc tcgaaatatc ccgcatctac ctctcggtag
    11341 gattggatcg acagtagcgc acccaaggtt tccgactgga cccgttccag ggcaatatcg
    11401 ctgtcgggct tcactgcggc ttctccgggc ccggcatctg cctcgccacc ttccgcagcc
    11461 gctcaaccca gcgggcgtcg tcctcatggc agcctgactc gcacatgaaa catccctttc
    11521 gcatccgggt gtcggccaac atacggctgg aggtcaggtg tcgcggaggg cggtgcgaac
    11581 ggcggcgagg taggtgcggc ctaccggcac ctgagcgccg ttggtcaggc gaagcgtcag
    11641 ctgctggccg tcgcggtcga agcttgcgat cgcctgccgc gcgacccacc agctgcggtg
    11701 gacgcgcagg ccgatcgccg cgtcgagaag ctcgaccgca tccgacaggc gcatcagcac
    11761 cagcgtcgag cccctgggcg tgtggacgcg gacataatgg tcctccgcct cgagcgcgac
    11821 gatttccggg ccgagtccgc ggggcatgcg cgcgagaagg accgccggga aggccggcgg
    11881 ggcgacggac ggcgcttcac tctcgcgggc gtcggcggcg cgctcggccg gggcgatggg
    11941 agccttgttc tccggcatct ccggctggtg cagggcggca gtgtggcccg gtgcgcccgc
    12001 atctggcgcg ttcggggcgg ccattgggtg caagggctgg gccggccgaa gcggcgccac
    12061 gaccggcctg atcagggcga cgatgatgac caattggacc gccgccacgc acgcaaaaag
    12121 gacgggaagc tggatcggcg ggaccacgcg ccccgggtgc acgagcgaga gcgtccaagc
    12181 gaccacgaag gtcatcggaa cggcggacgc cagcgcgatg aggccgttca tcgtgacacg
    12241 gccggcgcgg gcgaggccgg ggaatagttc ggagagccgg gcggcgccta gggcgaaggc
    12301 atagccggcc agaacgagcc cgatccagaa ggcgtaacgc accgggcggt cgagcgccgg
    12361 ataggttccg aacgggccag ccaagcccat gatcgccccg aagccgagga cgagcgccaa
    12421 ttgccgcagc tggtcgggaa tcccccgttc gcgcttcgcg agttgcatgg ttccggcgtt
    12481 gacgaaaagc ggaactctat tggcgaatat cgcgcttgtc gaggcggcgg cggatcgggc
    12541 ctgtcgcgcc cgaacaaagg gggactgacc gtggattggc gcttgcgtgc gaatgcccgc
    12601 gcctccgccc cggtcttgag cccctcgaaa ccagactgga cgccaatatg gaattcttcc
    12661 tcatcgtctt cctgccgatc gccttcttga cccttgtcgt cggcggcgcg gccgctttta
    12721 cctggcggtg cgcgcgttcg gcgggcgcga tctggatgtt ctgggcgccg gcggccgtca
    12781 tccatgcggg ttggtggctg ctgcccgagc tcgatgaagg cgtcgcggcc atcgtgcggt
    12841 ggttcgcgat cgtctggatc ggcgccatcc ttgccggcgc catcctgttc gtcccgttcg
    12901 cggcgatcaa agtggcgggc cgcctgtcgg ggcgcggcga ggcggatgac ggggtcatga
    12961 tcacgaccta cgggacgctc gcgctggctt tgggcttgat cgcgtcggtc ggaagcacca
    13021 gcgaggcccg tattcgcgag gagattgtcg cgatccggga tctgccggcg ggcctggacg
    13081 gggttcggat cgccaacctc gccgatgtcc atatcggacg gtttgtgacc gtcgccgatt
    13141 tgggtcgcgc gatccggcgc atcgatgccc gcggcgccga ttatctggtg gtgaccggcg
    13201 atctcatcga cgacttcagc cagctcgacc ggacgctggg ggcgctcgag accagcaagg
    13261 cgctgccggt tctgtcgatc ctcggcaatc atgagcggat gggcgacatt gaggccacgc
    13321 ttgccgctta tggccggcgg cggcggacgc gattgctgat caacgacctg gtggacctcg
    13381 agcatgaggg cgcggcgatc cgctttgtcg gcatcgacta tccgatggcc gaggatggcg
    13441 gccatgtcgc ctcgccggct cggcaggaca ggctgctgcg cgactttgcc ggcagggcgt
    13501 tcgcgcgagg ccaggggcgg gggctgacgg tggcgctttc gcatcatccg gggtttttcc
    13561 cttttgcggc cgcgcgtggg gctcggctca cgctggccgg gcacacccat ggcggccagg
    13621 tcgctttgtc cgggcggccg atcgtcgagg cctatcggta catggccggc cgctaccgga
    13681 tcggagaggc ccatctcgac gtgagccggg gctttggcca ctggctgccg ctcaggatcg
    13741 gcgtgccgag ggagaccgtg atcctgacgc tggtcagggc ctagagcccg gccgcctgaa
    13801 gcgttgaaaa atggtcgggg cgagaggatt cgaacctccg acccccacac ccccagtgtg
    13861 atgcgctacc aggctgcgct acgccccgac cgagggaccc caagcggggc ccggaagccg
    13921 atccccttag gcgcgggcga agatgatggc aagccagcgg cttgaccggg ccggagccga
    13981 agtatagttt caaaacgaaa ctgaaaggag ccgtcatgga gatacactac ggcctcgcca
    14041 cgcttgaaga gacgcgcggg ctcagtggga tcgaggcgct gagggcgatg atcgaagggc
    14101 ggctgccggc gccgccgatc gccaaggcaa tggggttccg gctggtcgaa gtgggcgagg
    14161 gcacggcggt gttcgagggc gagccggggc cgcatctcct caatccgctc ggcggggtcc
    14221 atggcggctt cgcgctgacc ctgatcgaca gcgccgccgg ctgcgcgctc cacgccgcgc
    14281 tcgaggcggg ggtcggctac gccacggtcg agaccaagac caacatgacc cggccgatcg
    14341 cgcccgacgg gggaatggtg cgcgccgagg gccggctgct gacgcgcggg cgccagatcg
    14401 ccaccgccga ggcgcggatc ctcgaccgcg agggccgcat cctcgcccac ggcacgtcga
    14461 cgctgatcat cctagcgccg cgcggctgag cgtcctccca cgcgcggccc gccttcgtca
    14521 ttccagcgaa agctggaatc tcttcgcctt gcgatctcgg cgccctgcca gggcatcgcc
    14581 agaagggaga tcccagcttt cgctgggatg acggggacgc cgccgcgccc gggcgggggg
    14641 ccttaacttg cgtgggcagg ggcaccatgt tagtcgcgcc cgcttgccgg gagggtcctc
    14701 ccgcacgtct ctcatcaggt aagacttcat gttcgcatcc cccgcttatg cagccacggg
    14761 cgccgccgcc tcgggagggg cgaccggctt cttcgtgtcg gtgttcccgc tggtcctcat
    14821 attcgtcatc ttctatttcc tgctgatccg gccccagcaa aggcggatga agcagcatca
    14881 ggcgatgatc gcggcggtca agaagaacga cgtggtggtg accggcggcg ggctggtcgg
    14941 caaggtcacc cgcgtcgacg agcatgaggt cgaggtcgag gtcgcgccca acgtccgggt
    15001 caaggcggtc aagggcacgc tcagcgaagt ccggccgcac ggcgccaagc ccgcgaacga
    15061 ctgatgctcg atttcccccg ttggaaggtc tgggcggtca gcctgttgat gctggtcggc
    15121 atcgccctcg ccgtgccgag cttcttcccg caatcggcgg tcgcccgctg gcccggcctg
    15181 ctgccgagcg cgcagatcaa cctcgggctc gatcttgcgg gcggcagcca cttgctgctc
    15241 caggccgaca ccagcggcgt cgcgcgccag cggctcgagc agatggagga gacgctgcgg
    15301 ctcgaaatgc ggcaggccga gccgcgcatc gagattggcg agatctccac cacgggcggg
    15361 cgggtgagct tcctggtccg caaccccgcc caagtcgatg cggcgcggga gatcgtctac
    15421 aaccagaccc agccggccgg gctgaccggg cagcgcgact ggaacgtcaa cgtcatcgac
    15481 accagccgca tcatcgtcga gccgaccagc gccggcctcg ccagcgcgct cgaaagcgcg
    15541 atgaacaccg cccgcgaggt ggtctataac cgcgtcgacc cggaagggac gcgcgaagtc
    15601 accgtggttc gccagggcgg cgaccgcatc ctggtccagg tccccggcct gcaggatccg
    15661 gaggggctga aggcgctgct cggcaagacc gcgcggctcg acttcaagct ggtcgacctc
    15721 agcgccgatc cggcgctggt cgcgcagggg cgctcgccgg ccggcagcca gaccctgccc
    15781 tatcccgaca acccgtcggg cgcgccgggg atcgcggtcc agcgccgcgc gatggtgacc
    15841 ggcgaccggc tggtcgacgc gacccagggc ttcgacccgc agaccaacga ggcggtggtc
    15901 aacatccgct tcgacggcgc cggcggcaag cgcttcgcca aggtgacgca ggagaatgtc
    15961 ggcaagccgt tcgccatcat cctcgacaac gtcgtcatct ccgcgcccaa catcaacgag
    16021 ccgatcctgg gcggccaggc gatgatctcg ggcagtttca cggtcgaaac cgcaaacgag
    16081 ctcgccgtgc aattgcgttc gggcaagctg ccggtcgatc tgaaggtggt ggaggagcgc
    16141 acggtcggac ccgatctcgg cgccgattcg atccggctcg gcgtcatcgc cgggaccgtc
    16201 ggcacgctcg ccgtgctggt cttcatgatc ctcacttatg gccggttcgg cgtctatgcg
    16261 gacattgcgc tggtgctgaa cgtggtgctg atcctcggca tcatggcgat cttcaacgcc
    16321 acgctgacgc tgccgggcat tgccggtttc gtgctgacga tcggcgccgc ggtcgacgcc
    16381 aacgtcatca tcaacgagcg catccgcgag gagcagaatc gcggccgcaa cgtgctgcaa
    16441 gcgatcgagc acggctataa ggaagcgcag accgcgatct tcgatgccaa catcaccaac
    16501 gtgatcgccg gcatcctgct cttctatttc ggcaccgggc ccgtgcgcgg cttcgcggtg
    16561 gtgatgatga tcggcatcgt cacctcggtg ttcaccgccg tctccgtgac ccgcatgttc
    16621 gtgtcgatct ggctgcgcaa gcggccgacg gagctcagga tatgagggcc gccgacaagc
    16681 agataccggc ttcggccgga gtgagggtcc gctgatgcgc ctgatgaagt tcgtccccga
    16741 gaacaccaat atcgacttcc tgcgctggcg gacggtggcg atctcgatct cggtgctgct
    16801 gatggcggcc tcgatcgcgc tgatcgcggt caaggggctc aatttcggag tcgactttgc
    16861 cggcggccag ctgatccgcg tctcgttcgc gcagaatgtc tcgctcgacg agctgcgcga
    16921 gcgggtgacc aatgtcgggc tcggcgagcc gagcatccag gagttcggca gcgccaagga
    16981 ggtggcgatc cggctgccgc tgcccgaagg cggcgaggag ggcgccaaca aagccgcctc
    17041 gaagcttcgg aagatgctcg tccagcaata tccgggcgcg cgcatcgacg cggtcgagac
    17101 ggtgtcgggc agggtgtcgg gcgagctggt gcgatcgggc gcgctggcgc tgctgctcgc
    17161 gatgatcggc atcgccatct atatctggat acgcttcgaa tggcagttcg gggtcggcgg
    17221 actgttctcg ctgctccacg acgtgaccct gacgctgggg ctgttttcgc tcacccagat
    17281 ggagttcgac ctcaacgtgg tcgcggcatt gctgaccctg atcggctatt ccttgaacga
    17341 cacgatcgtc atctacgacc gcatccggga gaatctgcga aaatatcgca agatggacat
    17401 tacctccctg ctcaacctgt cgatgaacga gacgctggcc cgcaccgtcg ccaccaacct
    17461 ttccatgctg ctggcgctcg gctcgctgat gttcctcggg cccgaggtca tccaaagctt
    17521 caccaccgcc ctcctcttca gcgtcgtcgt cggcacctat tcgaccgtct atatcgcctc
    17581 gcccttcctc atttggctca aggtcaattc ggacagcttc gtgccgaagg cgccggcgtc
    17641 gggcagcgcc gagcgggtgt cgcacaacga aggctattga tgccgcgctg ggaccgcgac
    17701 gcggccgctt ccgggccggt gatccggggc ttcaccgacc gcggcttcgc gatcgacggc
    17761 ggcgtctacc agggattgtt cctgaccccg gcgagcgcgc acgaatggcg gccgccggcg
    17821 ctcgccgacc tcaatgaaaa gcatctcgag ccggtgctgc acatcgaccc cgcccccgaa
    17881 ttcctgctgc tcggcaccgg cgcggcgctg gccttcccgc cgcgcgcgct catcaaggcg
    17941 ctcgacaagc gggggatcgg tctcgaagtg atggacagcc gcgccgccgc gcgcacctgg
    18001 ggcatgctcc gctcggagga gcgctggatc gtcgcggcga tcatgccgct ggcctgagcg
    18061 cccgcccggc cgcggccacg cccgcaaggt gatcgtataa agtgcggctg ttggtgtccc
    18121 agctgaaccg ctccaccacc ttgcgcacct cggcctgggc cggcggatcg gcgagcagct
    18181 cgcgcaccgc ggcggcgatc gcctccggtt cgagcggcgg cagccgcccg gcctcgggcc
    18241 gggtgaggac ttcgcgggcg ccgccgacgt cgccgatcac gatcggcgtg ccgcaggcga
    18301 gcgcttcgac ccagacgttg gcgaggcctt ccgaggccga gggcagcacc atcacgtcgg
    18361 cggcggcgaa gaggccgggc agctcctcat gggggcggtt gccgaggaag cggacgcggt
    18421 ccgcgacgcc ggccgccgcg gcatgcgcct cgagccggcg gcgctcggga ccctggccga
    18481 cgatcagcaa ggtggcgccg ggtatctggc ggagcgcctc gatcgccagc cgctggccct
    18541 tgcgggggat cagcgcgccg gccgacacga tcagcggtcc ttccacgccc aaggcggcct
    18601 tggcggcggc gcgggcgacg ggaatgaagc ggtcgaaatc gacgccggta taatggacct
    18661 tgatcttgcc gtcggggagg ccgagcgacg ccatcactcc ggccagcgcg ccgctgaccg
    18721 cgaggagccc gcccgccgcc cggccggcct cgaccagctg cggcgcgacc gcggggcggg
    18781 tgccccaatg attgatgtcg ccgccgcgcg ccttgaccga aaagggaacg ccgagcgcgt
    18841 ccgagaggat cttcgcggcc gggccgtcgg gccagaagaa ttgggcgtcg atcacgtcga
    18901 acggcaagtc cgcgcgaagg tcgcgcagca gcggcagcaa ggcggcggcc atggagcggg
    18961 cggtacgcgc ctcgcccagc cgcggcacca cgccgaagcg cgggcggtgg acgaccaggc
    19021 ctttccaggt ctcgcgaagc ggcagccgcg cgcgctcggc ataatggggg tggcgcgaca
    19081 gcggccaagg cggaagcccg accggggaga cgacttgcag ctcgaccccg ggcagcgccg
    19141 ccagccccaa agtctgccgc tcgacgaaaa ttccaaaggt gggctgcgcc gcgttcggga
    19201 acagcgtgga caaagtgagg acgcggagca tcaagcgggt ttagcagcgc caggcttgtc
    19261 atcgcattaa aaccgggtcc gccgcggctt ttgcggcttt gggctgccac gtcgcgcttg
    19321 gccttgcccc aagcctccgc taagaagccg atcatgcttc cgaaaaatta ccgcccgctc
    19381 gccctgttgc tcgccgccgc cgccacgctt ccgcttgccg gttgcgccgg ccggggcgcg
    19441 cgcgaaggcg ccgacaccgc ttatgtcgcg cgcgacgtga acacgctcta caccgccgcc
    19501 aagggcaggc tcgaccgcgg caattacaag caggccgcgg ctttgttcga cgaggtcgag
    19561 cgccagcatc cctattcggt atgggcgcgc cgggcgcagc tgatgagcgc gttcagctat
    19621 tatctggcca aggaccatac caagtcgatc gaatcggcgc ggcgcttcct gtcgatccat
    19681 cccggcaaca aggacgcgcc ctacgcgctc tacctggtcg cgctcaatta ttacgagcag
    19741 atcgaggatg tgaaccgcga ccagagcatt tcgaaacagg cgctggaggc gctgggcgag
    19801 ctggtccggc ggcacccgac cacgcggttc gcggccgatg cgcggctcaa ggtcgatctg
    19861 gtccgcgacc atctcggcgg caaggagatg gagatcggcc gcttctacca gcgccgcaag
    19921 cagtggctcg cctcgatcac ccgtttccgc accgtgatcg acgaatatca gacgacgacg
    19981 cacacccccg aagcgctgat gcggctcacc gaatcctatc tcgcgctcgg catcaccgac
    20041 gaggcgcgca aatccgcggc cgtgctcggc gccaatcatc ccggcagcaa atggtatcag
    20101 cgcgcctatc agctggtgca gcggcacggc gccccgcccg ccgcggcggc cccggcggcc
    20161 gcaccggcgg cctgaatgcc cgaccgtccg tgctgaaccc gtttcagcat ccggcgtgac
    20221 ggcggcgagg gggcgcgtct gttaacctcc ctttcgatcc gcgacgtcgt gctcattgag
    20281 acgctcgacc tcgaattcgg ccgcggcctc ggcgtgctga caggcgagac cggggccgga
    20341 aagtcgatcc tgctcgacgc gctcggcctc gcgctcggcg cgcgcgccga cagcctgatg
    20401 gtccgcgccg gccaggccca ggcggtggtc agcgtcagct tcgacattcc gcgcgcccat
    20461 cccgcgctgg ggctgctcga cgagaacggg cttggcggtg agcccggcga gccgctggtc
    20521 atccgccgca tcctcaaggc cgacggcggc agccgcgcct tcgtcaacga tcagtcggcc
    20581 tcggtcgccc tgctccgcga gctcggcccg atgctggtcg agatccacgg ccagcatgac
    20641 gatcgcggcc tgctcaaccc gcgcggccac cgcgcgctgc tcgacagctt cggcggcatc
    20701 gaccgcgctc cttgcgacgc ggcgttccgc gcctggcgct cggccgaggc gcggctcgcc
    20761 gccgcccgcg agcagctcga aatcgccgcc cgcgaccgcg agtggctcga ccatgcggtc
    20821 gccgagctgg tgaaattggc gccgcgagcg ggcgaggagg cggagctcgc cgacgcccgc
    20881 gcccagatgc agaagggcgc gcgcctggcc gaggatatcg cgctcgtggc gcagacgctg
    20941 gacggttccg acggcgggct ggcgctgctg cggcaggcgg cgcggcggct ggagcgggtg
    21001 gcgggcgagg atgagcggct gggcgaggcg ctggcggcgc tggaccgcgc ggtcaatgag
    21061 gcttgcgagg ccgaggaccg gctcggcgag gcggccgagg cgatggcgtt cgatccggcg
    21121 cggctcgagg aatccgagac gcggctgttc gagctgcgcg cgatcgcccg caagcaccgg
    21181 gtcgatcccg aggcgctgcc ggcgctggcg gaggagctgg ccgagcggct ggtgggaatc
    21241 gaggcgggcg atgccgggct ggcggcgatg gaaaaggggc tgcgggccag ccggctcgct
    21301 tatgaa
//
LOCUS       DASVRK010000282        17555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_602676, whole genome shotgun sequence.
ACCESSION   DASVRK010000282 DASVRK010000000
VERSION     DASVRK010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 17555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17555
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602676"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF589_11500"
     CDS             complement(<1..317)
                     /locus_tag="VF589_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX8258248.1"
                     /translation="MPTIISHAAVPLAIGLGLGRRRLPAALVAAGAAAAMLPDLDVIG
                     FRFGITYGDAFGHRGASHALAAAALLAFAAAWPFRSAAVPFGRAFLFLFAAAASHGLT
                     DMLT"
     gene            complement(325..957)
                     /locus_tag="VF589_11505"
     CDS             complement(325..957)
                     /locus_tag="VF589_11505"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX8258249.1"
                     /translation="MILVVDNYDSFTWNIVHYLRELGAAVEVVRNDEVSAAGAMASAA
                     EGLLISPGPGNPDEAGMSLELVGLCAAARRPLLGVCLGHQAIGQYFGGIVVRAGAPMH
                     GKTCPLEHDGSGVFEGLPSPFTATRYHSLAVAAAGLPDCLLVNATADDSTVQGLRHRA
                     LPIHGVQFHPESVASEHGHRLLANFLRIVAAEAGRQPGAAEAWTGGRAAL"
     gene            complement(994..2943)
                     /locus_tag="VF589_11510"
     CDS             complement(994..2943)
                     /locus_tag="VF589_11510"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX8258250.1"
                     /translation="MLSMFRRGVTSKIMLVILAIGLFAIVATGFGTDGMGLGGLSGGG
                     AGETVASVEGEKITSSELVDRVNDTLQQAREQQPELDMGSFLREGAVEKILNQMIFSR
                     SLALFGRGQGLAASDKMIDTQIASIPAFRNLAGQFDPNAFRARLQQEGLSEQELREDL
                     ANSLIYDQIRTPISAAPSVPKTMALQYAALLLEQRTGAVGVVPSAAMGSGTPPSDAEV
                     TAYYRENLSRYTIPERRVLRYATIGSAQVAQAAQPTEAEVNAFYQRSSERYGAKETRT
                     LSQVVLPDQAAARAFAAKLAAGRSFDAAATEAGFSAEDTRVGPQSKAQFTELTSPAVA
                     NVAFAAAEGTTTAPGQSPLGWHIVRIDGIARQAATPLAAVRGEIERQIGQQKQAEALA
                     ALVVRVEDAIAGGASLEEVARAEKLAVSETPPITAAGVQPGNPGWRAPELAPLLDTAF
                     DMTPDDDPVVETLQPNQSFALVAVSRVIPSAAPPLAEVAPRVRTDLTARRAADRARAV
                     ATSIAAKINAGVPSRQAFAEAKLALPPVQAVTAKRIDIARPDQPPPPPLLMLFTLPEG
                     KARILRAPGGQGWFVVHHEKRVPGNAASAPQLVDATRIQFERVLGEEYAEQFGRAVEA
                     GMDIERKPEALGAVRRQLLGPGSAP"
     gene            3065..3808
                     /gene="tpiA"
                     /locus_tag="VF589_11515"
     CDS             3065..3808
                     /gene="tpiA"
                     /locus_tag="VF589_11515"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX8258251.1"
                     /translation="MRRKLIVGNWKMNGSLGQLAEVSAISAAARKAAGVDVAICPPFT
                     LIAAAIPRSAGLPIGAQDCHARDHGAHTGSVSAAMLKEAGAKLVIVGHSERRAEQHET
                     DDAVRAKAEAAQRHGLQAIVCVGESAEQRAAGRAVEIVTAQLRGSVPDAGGDGELVVA
                     YEPIWAIGTGNVATPADVGEMHTAIRGFLRDWFGERGGRTRILYGGSVNAGNAAETFG
                     VDDVDGALVGGASLTAAQFVPIIEAAARP"
     gene            4086..4496
                     /gene="secG"
                     /locus_tag="VF589_11520"
     CDS             4086..4496
                     /gene="secG"
                     /locus_tag="VF589_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX8258252.1"
                     /translation="MFTFLLIVQTLVAATLVTVILMQRSEGGGLGVGGSTSGLMTARG
                     AADFLTRATAVLATLFVLLSIVLAGLAAVDRAPNPIDPSVAAAPPPAPAPVGSVAPVP
                     GQVQGAEGDPLAGAAAAAGNGAAPANSSDVPLAQ"
     gene            complement(4499..5800)
                     /locus_tag="VF589_11525"
     CDS             complement(4499..5800)
                     /locus_tag="VF589_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX8258253.1"
                     /translation="MSAMGQADSARPRSAVSPAVGLVGLAGLLAWTAAAFALGMAGPL
                     AALCGLVACGVPMIVWSFLVDKVHRSPTSGIDWEAPPRPLTETMPTSLVKIAGLWATW
                     AGIGFLYCVGRWYWEGQYLFAMEVLGLGLAPLVLASVPYVIWLDRRLNEPRDGAWHFG
                     CLVIGRHGLAERERIYEHLRCWAIKAFFLAFMLAVLPGNWFNVIRPGGAEIVANPVAL
                     TIWIANLTFMVDVSFATLGYMLTLKPLDAHIRSANPYAAGWTAALLCYPPFSAGGVGG
                     PLNYHPGSADWAYWMEGWPALLALYAVPLIALHIIYAWATVAFGLRFSNLTHRGILTH
                     GPFAWTKHPAYLSKTLFWWMASLPFLAANGNPVDMVRNTMLLAGVSGIYYWRARTEER
                     HLMADPAYRDYAAWMALNGPLARLARHLRRPAREPELAAAE"
     gene            6024..7691
                     /locus_tag="VF589_11530"
     CDS             6024..7691
                     /locus_tag="VF589_11530"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX8258254.1"
                     /translation="MARFIFITGGVVSSLGKGLMAASLAALLQARGYKVRIRKFDPYL
                     NVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVPARQSDNITTGRIYRDIIMKE
                     RRGDYLGATVQVIPHVTNEIKAFARTDLGDLDFLICEIGGTVGDIESLPFIEAIRQLR
                     NDLGTDEQGRKNAVFIHTTLVPYIAAAGELKTKPTQHSVRDLTSLGIQPDVLLCRVDR
                     PLPAGERAKIAQFCNVRTEAVIPALDAKSIYSVPLQYHREGLDAEVLRAFGLAPDPDG
                     DTPAPDHARWLDILDRLENFEGEVTIGVVGKYVGLQDAYKSLTEALVHGGLANRVKVN
                     IRWLDAELFEHPEADIAPELEPMHAILVPGGFGERGSEGKIASVQFARERRVPFFGIC
                     LGMQMACIEGARNTAGIAGASSTEFGDCAEPVVGLITEWMSAEGLRQRREANGDMGGT
                     MRLGAYEARLGGNSVVRSIYGEDVISERHRHRYEVNVHYKGALEKGGLVFSGMSPDGA
                     LPEIVERPDHPWFVGVQFHPELKSKPFEPHPLFASFIEAAVKQSRLV"
     gene            8643..9008
                     /locus_tag="VF589_11535"
     CDS             8643..9008
                     /locus_tag="VF589_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX8258255.1"
                     /translation="MTGSRTDEEQIGEHGGGFGIRDLGLFQSAMARPLNLAAYGEPDL
                     AALASAYAYGIARNHPFIDGNKRTAAVVGETFILLNGGRLEVDDAELAVVFLALAAGE
                     LSEEALAEWFRERIIASQE"
     gene            9068..9499
                     /locus_tag="VF589_11540"
     CDS             9068..9499
                     /locus_tag="VF589_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258256.1"
                     /translation="MIISRGNGNVAEKAERPAPPPKPRVPAAELPAKPVETPVPAADF
                     RLVHHRLAALERLAALLEKGALDAAEFAAEKALILRLPAEELVLGPDRAARPPRGPSL
                     LGRLLGWRVLAAGAALGLAVSFATQPQELLTVAERAGRLLG"
     gene            complement(9501..10259)
                     /locus_tag="VF589_11545"
     CDS             complement(9501..10259)
                     /locus_tag="VF589_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX8258257.1"
                     /translation="MTLSDDQLDRYASHIVLREIGGEGQRKLLAASVTVIGAGGIGSP
                     AIQYLAAAGVGRLRVIDDDSVTLDNLQRQVLFGTGDVGRGKVEVSGEAVARLNPGVAF
                     EGVRERLSSADAARLIDGSDVVIDGSDNFATRLAVSDACTAMRIPLVSAAIGQFQAQV
                     GTWRGWEAGRPCYRCFVGDAFDAEDCDTCSELGVLGAMAGIAGSWAALEAVRQIVGFG
                     EEAAGKIHIFDGLKPGLRTLRVGKDPACRACSAA"
     gene            complement(10256..10705)
                     /gene="dut"
                     /locus_tag="VF589_11550"
     CDS             complement(10256..10705)
                     /gene="dut"
                     /locus_tag="VF589_11550"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX8258258.1"
                     /translation="MPFSEIRISLKRLPHGADLPLPCYATAHAAGLDVVAAESLTLAP
                     GARHAVATGFAVAIPEGYEVQVRPRSGLALKHGITCLNTPGTIDADYRGEVKVILANL
                     GAEPFEIVRGERIAQLVPAPVQRAIFAEVENLDATERGAGGFGSTGR"
     gene            complement(10692..11897)
                     /gene="coaBC"
                     /locus_tag="VF589_11555"
     CDS             complement(10692..11897)
                     /gene="coaBC"
                     /locus_tag="VF589_11555"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX8258259.1"
                     /translation="MNSKRILLIVGGGIAAYKSAELIRLLRKDGLEVTCVLTEGGSHF
                     ITAMTLGALSENKVYTSLWDLKDEVEMGHIQLSRQADLVVVCPATADLMAKMAAGIAD
                     DLATTLLLATDKPVLAAPAMNVRMWRHEATRRNVEQLRRDGVTVLEPDDGEMACGEYG
                     PGRLPEPERILAAIREQLSGQKSSGAPHTAGSLAGRHVLVTAGPTHEPIDPVRVIANR
                     SSGKQGFAIAEALAELGARVTLVAGPVSLATPPGVERVDVETARQMDAAVRAALPADA
                     AVLVAAVADWRVEPAPSKLSKADGPPELAWSANPDILLGLAESRHRPRLLVGFAAQTD
                     AVIEAARAKRHRKGCDWIVANDVSKGVMGADENTVHLITADGVESWDKAPKQDVARHL
                     ADRIADALQ"
     gene            12442..12996
                     /locus_tag="VF589_11560"
     CDS             12442..12996
                     /locus_tag="VF589_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258260.1"
                     /translation="MWLGTPSSLQYVVAPDEEAISLETEGANMASVRPIAIGREMRVT
                     LLPHWNFELLSPNGEKKPLGLDESATWQWQVRPKEQGGPFEILAEVQVFGPDRDGKAT
                     VLDTYTRRVAVQVHVGTWNGFLNALAKAKTLGELFQALFATWKSTLISLSALLAALGG
                     VIVAVRTLGSKKGQETREQTPAPD"
     gene            complement(12993..14525)
                     /gene="ubiB"
                     /locus_tag="VF589_11565"
     CDS             complement(12993..14525)
                     /gene="ubiB"
                     /locus_tag="VF589_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017180990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEX8258261.1"
                     /translation="MTAPAVHIWRLLKWGRVLARHGALRGIERDPNTPPPVRRLARIA
                     RFGARVPASPTYAEAFQEIGPAAIKFGQALATRPDLVGDEAARNLSLLQDSLPPAPFE
                     QIRRVIEQGLEKPIDQAFSHIDPEPVGAASIAQVHRAVTTDGQQVAVKVLRPGIEDDF
                     ARAMDTYEWAAAQVESMGGEAARLRPRMVIAHFRQWTARELDLRREAASASELRENLV
                     AESGFFVPAIDWSRTSRRVMTLEWIDGIKLTDRDALVAAGHDLKALAAILVRAFLRQA
                     VIDGFFHADLHQGNLFALPDGRLAAIDFGIMGRVDRQARMWLAEILYGLIVGNYRRVA
                     EIHFEAQYVPSHHSVEEFATALRAAGEPIRGLPVKDISVAQMLDSLFTITRDFDMPTQ
                     PHLLLLQKTMVMEEGVATALDPDINMWETSEPFLREWLRSELGPEAALADRLVRDFRT
                     LAGLPDLIRRLDHYYPPPGAAPPPPPLPDVVVVRPRRYGSYFLTALIAAAVGALLAQL
                     FV"
     gene            complement(14614..15342)
                     /locus_tag="VF589_11570"
     CDS             complement(14614..15342)
                     /locus_tag="VF589_11570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8258262.1"
                     /translation="MDKVSFGYEDVPPEEKTQRVGRVFSNVARRYDLMNDLMSGGLHR
                     HWKNQFVRRLRPRARETILDMAGGTGDVAFRIAASGAQVTVADINPDMLAVGIERSRR
                     QGVPGLLWAEENAEALSFPDETFDAYTIAFGIRNVTDIGAALAEAHRVLRIGGRFFCL
                     EFSTTSWPGFAQLYDRYSHEVVPRIGKAVADDADSYRYLVESIRRFTNMPAFKAMIEQ
                     AGFARVSVEPILGGLVAIHSGWKI"
     gene            15390..16202
                     /gene="mutM"
                     /locus_tag="VF589_11575"
     CDS             15390..16202
                     /gene="mutM"
                     /locus_tag="VF589_11575"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX8258263.1"
                     /translation="MPELPEVETTVRGLVPVLEGRRLASVETRRPDLRRPFPPDLRQR
                     LTNATVTGLGRRAKYGLIGTDRGDTLIFHLGMSGRWRIDPAELGPHDHVLIATEEGRR
                     LALNDPRRFGSLDLVPTADLGAYPPFVAMGPEPLGEAFSPAYLAGALKGRSAPIKALL
                     LDQRIVAGLGNIYVCEALHMAGIAPGRAGGRIGGERLARLVEAVKTVLLAAIEAGGSS
                     LRDYVRPDGELGYFSKQWRVYGREGESCGCGVPIRRRAEGGRSTFYCAACQR"
     gene            16297..16560
                     /gene="rpsT"
                     /locus_tag="VF589_11580"
     CDS             16297..16560
                     /gene="rpsT"
                     /locus_tag="VF589_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX8258264.1"
                     /translation="MANTPQAKKRIRRNDRRAEINGARVSRIRTFVKQLEAALATGDK
                     DQATAAFSRVQPELARGVAKGVVHKNTASRKISRLSKRVAALG"
     gene            16973..>17555
                     /locus_tag="VF589_11585"
     CDS             16973..>17555
                     /locus_tag="VF589_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX8258265.1"
                     /translation="MNHSPAKGVALPANEGVAEPIAAAWEQIRAGLRRDCGARTFDGW
                     LRPIALAGFDPDSATVRLELPSQFMADWVQTHFAERLGLAWRTMLPLVRDVVIGVGAG
                     AGQRTVTALEIPPPPAEPVSPAEQPGGLYAAQLEPRYRFETFVVGKANEVAFNAARTL
                     AAADNVAFNPLFLHGGTGRGKTHLLHAIGHEFRA"
BASE COUNT         2791 a         6200 c         5840 g         2724 t
ORIGIN      
        1 gtgagcatgt cggtgagccc gtgcgaggcg gccgcggcga acaggaacag gaaggcgcgc
       61 ccgaacggca cagccgcgct ccggaacggc caggcggcgg cgaaagccag caaagcggcg
      121 gcggcgagcg catggctggc gccgcgatgg ccgaaggcgt cgccatacgt tatgccgaac
      181 cggaagccga tcacgtcgag atcggggagc atggccgccg ccgcccccgc cgccaccaaa
      241 gcggccggca accggcgccg cccgagcccg agcccgatcg ccagcggcac cgcggcatgc
      301 gagattatgg tcggcatccc cgctttatag agccgcgcgc ccgccggtcc acgcctccgc
      361 cgcgccgggc tgccgccccg cttccgccgc cacgatcctg aggaaattgg cgagcagcct
      421 gtggccatgt tcgctggcca cgctttccgg gtggaactgg acgccgtgga tcggcagcgc
      481 gcggtggcgc aggccctgga cggtggaatc gtccgcggtc gcgttgacca ggaggcaatc
      541 gggcagcccg gccgccgcca ccgccagcga atgatagcgg gtcgcggtga acggggaggg
      601 gaggccttcg aagacgccgc tgccatcatg ttcgagcggg caggtcttgc cgtgcatcgg
      661 ggcccccgcg cgtacgacaa tgccgccgaa atattgcccg atcgcctgat ggccgaggca
      721 cacgccgagc aaaggccgcc gcgccgccgc gcatagtccg accagctcca gcgacatccc
      781 ggcctcgtcg gggttccccg ggccgggcga gatcagcagc ccctcggccg cgcttgccat
      841 cgccccggcg gcgcttacct cgtcgttccg caccacctcc accgccgcgc caagctcgcg
      901 cagataatgg acgatgttcc aggtgaagct gtcataattg tcgacgacca ggatcatgcc
      961 caaagcttcg cccggctccc gaaccaaacg gcgtcagggc gccgagccgg ggcccagcag
     1021 ctgccgccgc acggcgccca gcgcctcggg cttgcgctcg atgtccatgc ccgcctcgac
     1081 cgcgcggccg aactgctcgg catattcctc gcccaatacc cgctcgaact ggatccgcgt
     1141 cgcatcgacc agctgcggcg cgctggcggc attgccgggc acccgtttct cgtgatgcac
     1201 cacgaaccag ccttggccgc cgggcgcgcg caggatccgc gccttgccct cggggagggt
     1261 gaacagcatc aacagcggcg gcggcggcgg ctggtcgggc cgggcgatgt cgatccgctt
     1321 ggcggtcacc gcctgcaccg gcggcagcgc cagcttcgcc tcggcaaagg cctggcgcga
     1381 cggaacgccg gcgttgatct tggcggcgat cgaggtcgcc acggcgcgcg cgcggtcggc
     1441 ggcgcggcgt gccgtcaggt cggtccgcac ccgcggcgcg acctcggcca gcggcggcgc
     1501 cgccgacggg atcacgcggc tcaccgccac cagggcgaag ctctggttcg gctgcagcgt
     1561 ctcgaccacc gggtcgtcgt ccggggtcat gtcgaaggca gtatcgagca gcggcgcgag
     1621 ctcgggcgcg cgccagcccg gattgcccgg ctggacgccg gcggcggtga tcggcggcgt
     1681 ctcgctgacg gcgagcttct cggcccgcgc cacttcctcc aggctggcgc ccccggcgat
     1741 cgcatcctcg acccgcacca ccagggcggc gagcgcttcg gcctgcttct gctggccgat
     1801 ctggcgctcg atctcgccgc ggaccgcggc cagcggagtc gcggcctggc gggcgatgcc
     1861 gtcgatgcgg acgatgtgcc agccgagcgg cgactggccc ggcgcggtcg tcgtgccctc
     1921 ggccgcggcg aaggcgacgt tggcgacggc cggcgaggtc agctcggtga attgcgcctt
     1981 gctctgcggc ccgacgcgcg tgtcctcggc gctgaagccg gcctcggtcg ccgccgcgtc
     2041 gaagctgcgc ccggcggcga gcttggccgc gaaggcgcgc gccgccgcct ggtcgggcag
     2101 caccacctgg ctcagcgtcc gggtctcctt ggcgccgtag cgctcgctgc tgcgctggta
     2161 gaaagcgttg acctcggcct cggtcggctg cgcggcctgc gccacctgcg ccgatccgat
     2221 cgtcgcgtag cgcagcacgc gccgctcggg gatcgtgtag cggctcagat tctcccgata
     2281 ataagcggtg acctcggcat cgctcggcgg agtcccgctg cccatcgcgg ccgacggcac
     2341 cactccgacg gcgccggtcc gctgctcgag cagcagcgcc gcatattgaa gcgccatcgt
     2401 tttcggcacg ctcggcgccg ccgagatcgg ggtccggatc tggtcgtaga tgagcgagtt
     2461 ggcgaggtcc tcgcgcagct cctgctcgct aaggccttct tgctgcaggc gggcgcggaa
     2521 ggcgttggga tcgaactgcc cggccaggtt gcggaaggcc gggatggacg cgatctgcgt
     2581 gtcgatcatc ttgtccgacg ccgccaggcc ctgcccgcgc ccgaacaagg ccagcgagcg
     2641 cgagaagatc atctggttca ggattttctc gaccgcgccc tcgcgcagaa aggaccccat
     2701 gtcgagctcg ggctgctgct cccgcgcctg ctgcagcgtg tcgttgaccc ggtcgaccag
     2761 ctcggacgag gtgatcttct cgccctccac gctggccacc gtctcgccgg ccccgccgcc
     2821 ggagaggccg cccagcccca tgccgtcggt gccgaagccg gtcgcgacga tcgcgaacag
     2881 gccgatggcg aggatgacga gcatgatctt ggacgtgacg ccgcggcgga acatggaaag
     2941 catgggtttt ccggatatgt gaggtgaagc cggcgccgtc atagaagcgg ccatccgctc
     3001 cggcaagctt ggctccgcgc ccgccgcttg ctaggcccgg cgcgaagcgg gaggaatcgt
     3061 caaggtgcgt cgcaagctta tcgtcggcaa ttggaaaatg aacggtagtc tcggccaatt
     3121 ggccgaagtg tcggcgatct cggccgcggc ccgcaaggcg gccggcgtcg acgtcgccat
     3181 ctgcccgccc ttcaccctca tcgccgccgc catcccgcgc tccgccgggc tgccgatcgg
     3241 cgcgcaggat tgccacgcca gggatcacgg cgcccatacc ggcagcgtgt cggctgcgat
     3301 gctcaaggaa gccggcgcga agctggtcat cgtcggccat tccgagcgcc gcgccgagca
     3361 gcacgaaacc gacgatgccg tccgcgccaa ggccgaggcc gcccagcgcc acggcctcca
     3421 ggcgatcgtc tgcgtcggcg aaagcgcgga gcagcgcgcg gcggggaggg cggtcgagat
     3481 cgtcaccgcc cagctgcgcg gctcggtccc cgacgccggc ggcgacggcg agctggtcgt
     3541 cgcctacgag ccgatctggg cgatcggcac cggcaatgtc gccacccccg ccgacgtcgg
     3601 cgaaatgcac accgcgatcc gcggcttcct gcgcgactgg ttcggcgagc gcggcggccg
     3661 cacccgcatc ctctatggcg gctcggtcaa tgccggcaac gccgccgaaa cgttcggcgt
     3721 cgacgatgtc gacggcgccc tcgtcggcgg cgccagcctc accgccgccc agttcgtccc
     3781 gatcatcgaa gccgccgcgc ggccctgagt cccgcagtcc cagcgacact caaccgtcat
     3841 cccagcgaaa gctgggatct cacttcttct tccgatgagc cggctaggaa cacccggatt
     3901 gtcgcctccg cggcaaccac cagacggcga agcttgaacc gcacccccgc ttcgcctaga
     3961 tcgcgccgca ggtcatcccg gcgaaagccg ggatccaggc tgcctccgtg cccgcacgag
     4021 ctccccctgg cccccggctt cccgccgcgg gcgaccggca attccgattt cgaggcccgc
     4081 gcaacatgtt cacctttctc ctcatcgtcc agaccctggt cgccgcgacg ctcgtcaccg
     4141 tcatcctgat gcagcgctcc gaaggcggcg ggctcggcgt cggcggctcg acctcggggc
     4201 tgatgaccgc ccgcggcgcc gccgatttcc tgacccgcgc gaccgcggtc ctcgccacct
     4261 tgttcgtgtt gctgtcgatc gtgctggccg gcctcgccgc cgtcgaccgc gcgcccaacc
     4321 cgatcgaccc gtcggtcgcc gccgccccac cgcccgcgcc cgcgccggtc ggttcggtcg
     4381 cgccggtgcc cggccaggtt cagggggccg agggcgatcc gctcgccggt gccgccgcgg
     4441 ccgccggcaa tggcgccgcg cccgccaact caagcgacgt gccgctcgcc cagtaaaatt
     4501 attcggcggc ggcgagctcc ggctcgcgcg ccggccggcg caggtgccgc gccagccgcg
     4561 ccagcggccc gttcagcgcc atccacgcgg catagtcgcg ataggcgggg tcggccatca
     4621 gatgccgctc ctcggtccgg gcgcgccaat aatagatgcc gctcaccccg gccagcagca
     4681 tggtgttgcg caccatgtcg accgggttgc cgttggccgc caggaacggc agcgacgcca
     4741 tccaccagaa caaagtcttc gacagatagg ccggatgctt ggtccaggca aaagggccgt
     4801 gggtcaggat gccgcgatgg gtgaggttgg agaaacgcag cccgaacgcc accgtcgccc
     4861 aggcgtagat gatgtggagc gcgatcagcg gcaccgcata gagcgccagc aaggccggcc
     4921 agccctccat ccaataagcc cagtcggccg accccggatg atagttgagc ggcccgccga
     4981 cgccgcccgc ggagaaaggc gggtagcaga gcaaggccgc cgtccatccg gccgcatagg
     5041 gattggccga gcggatgtgc gcgtcgagcg gcttcagcgt cagcatatag cccagggtcg
     5101 cgaagctcac gtcgaccatg aaggtgaggt tggcgatcca gatcgtcagc gccaccgggt
     5161 tcgccacgat ctcggccccg cccggccgga tcacattgaa ccaattgccc ggcagcacgg
     5221 ccagcatgaa ggcgaggaag aaggccttga tcgcccagca ccgcagatgc tcgtagatcc
     5281 gctcgcgctc ggcgagcccg tgccgtccga tgacgaggca gccgaaatgc caggcgccgt
     5341 cgcgcggctc attcagccgc cggtcgagcc agatcacata ggggacggag gcgaggacca
     5401 gcggcgccag ccccagcccc agcacctcca tcgcgaacag atattgcccc tcccaatacc
     5461 agcggccgac gcaatagagg aagccgatgc ccgcccaggt cgcccacagc cccgcgatct
     5521 tgacgaggct ggtcggcatc gtctcggtta gcggccgcgg cggcgcctcc cagtcgatcc
     5581 cgctggtcgg gctccggtgg accttgtcga ccaggaacga ccagacgatc atcggcaccc
     5641 cgcaggcgac gaggccgcac aaggccgcca gcggccccgc catgccgagc gcgaacgccg
     5701 ccgccgtcca ggcaagcagg ccagcgagcc cgaccagccc taccgccggg ctcaccgccg
     5761 agcgcggccg cgcgctgtcc gcctggccca tcgcgctcat ccgcgccgct ccgcccgcac
     5821 aaggttcatc cgcccggaat agcagcactt tccctcgcga ccgaggcccc gccgccgcct
     5881 ttcgcgccgc tgtgccgctt cggaacagcc gcggcggcct gtctgaaccg ccgccccccg
     5941 gggccgcccg cgacgaataa attcgcccgc gccgattctc ccgcttgccc cggccccgtc
     6001 ccaccgctta agcctaacct cccatggcgc ggttcatttt catcaccggc ggcgtggtct
     6061 cctcgcttgg caaggggctg atggcggcat cgctcgccgc cctcttgcag gcgcgcggct
     6121 acaaggtccg catccgtaag ttcgacccct atctcaacgt cgatccgggc acgatgtcgc
     6181 cctatcagca tggcgaggtc tacgtcaccg acgacggcgc cgagaccgac ctcgacctcg
     6241 gccattacga gcgcttcacc ggcgtccccg cccgccagtc ggacaacatc accaccggcc
     6301 gcatctaccg cgacatcatc atgaaggagc gtcgcggcga ttatctcggc gccaccgtcc
     6361 aggtcatccc gcacgtcacc aacgagatca aggccttcgc ccgcacggat ctcggcgacc
     6421 tcgatttcct gatctgcgag atcggcggca ccgtcggcga catcgaatct ttgcccttca
     6481 tcgaggccat ccggcaactc aggaacgatc tcggcaccga cgagcagggc cgcaagaacg
     6541 ccgtcttcat ccacaccact ttggtcccct acatcgccgc cgccggcgag ctgaagacca
     6601 agcccaccca gcatagcgtg cgcgacctca ccagcctcgg catccagccc gacgtcctcc
     6661 tgtgccgcgt cgaccgcccg ctccccgccg gcgagcgcgc caagatcgcc caattctgca
     6721 atgtccgcac cgaggcggtg atcccggcgc tcgacgccaa gtcgatctat tcggtcccgc
     6781 tccaatatca tcgcgaaggc ctcgacgccg aagtgctgcg cgccttcggc ctcgcccccg
     6841 atcccgatgg cgacacgccc gcgcccgacc atgcccgctg gctcgacatc ctcgaccggc
     6901 tcgagaattt cgaaggcgag gtgacgatcg gcgtcgtcgg caaatatgtc ggcctccagg
     6961 acgcctataa gagcctcacc gaggcgctgg tccatggcgg cctcgccaac cgcgtcaagg
     7021 tcaacatacg ctggctcgac gcggagctgt tcgagcatcc cgaggccgac atcgctcccg
     7081 agctcgagcc gatgcacgcc atcctcgtcc ccggcggctt cggcgagcgc ggctcggagg
     7141 gcaagatcgc ctccgtccag ttcgcccgcg agcgccgcgt ccccttcttc ggcatctgcc
     7201 tcggcatgca aatggcctgc atcgaaggcg cccgcaacac ggcgggcata gcgggcgcca
     7261 gttcgaccga attcggcgac tgcgccgagc ccgtcgtcgg cctcatcacc gaatggatga
     7321 gcgcggaagg gctgcgccag cgccgggaag ccaatggcga catgggcggc acgatgcggc
     7381 tgggcgccta tgaggcgagg ctaggcggca acagcgtggt ccggagcatc tacggcgagg
     7441 acgtgatctc ggagcggcac aggcaccgct acgaggtcaa cgtgcactat aagggggcgc
     7501 tggagaaggg cgggctggtc ttctccggca tgagccccga cggcgcgctg ccggagatcg
     7561 tcgagcgtcc ggaccatccc tggttcgtcg gggtgcagtt ccacccggag ctgaagagta
     7621 agccattcga gccccaccca ttgttcgcga gctttatcga ggcagccgtg aagcaatctc
     7681 gccttgtttg acggctccac agaccgaccg tcctgagctt gtcgaagacg gcggcggtca
     7741 agcggtctcg cctcgtctag ctcgccccgg aacgaagagg ggaccgcacg aaatggtcag
     7801 ccgccatgtt cccaggacat ggctggaaag tgcggggcac tgtccacctg accatggtgg
     7861 aggggccggc gcagccggcg gtgaggttca aataaccgcc aaaacccgct aatccggagc
     7921 ggagccgacc agagctggtt ggtgcgccat ttggcgcgtc ttccaggtag ccggtgtggg
     7981 attggagaga tactccgtat aaccgctgca cttgagcctc cgtcgaaccg acaccgattc
     8041 ctcgcggcgg tttgactctg ctcgagccgg tgatggagat gcgcgcgagc ctgttcctgt
     8101 cgaataggaa ctccatgctc tcgtctcctc cggccatggt cgtctgcacg cactcttcag
     8161 gatcgtgaga actccttctc tcgaccgtca accgtgcgcc gatcgcgcgt gacacttgag
     8221 cgcgtgtcat tcccatccga accggcccgt acccctcggg cgtcaaacgc caaggcttcg
     8281 actgagcgaa cgctgcagat gccgtggctg caagcgcggt cgaggctgca aaaattcgca
     8341 ttgctcttac ggtcgggcga cgacgaactc cgtcaccccg tctcaacgca cgtcgaccca
     8401 accgctttcg cgcttctcga acgtgagcac tccattgtac gcagtggtgg caccgcgtgg
     8461 ctgaggccgg ctacctccca ttcgagaaga agattgaagc gcgcagttga agcccatgaa
     8521 tcggttcccc ctacttacgc actctggacg atagccgttc gggaagtgga ccgtagccgt
     8581 gtagaaaaag cggtaaccgc ggacgccgaa catctcgacc ccttgaccgt cagtctttct
     8641 gaatgactgg atcgcgaacg gacgaggagc agatcgggga gcatggcggc ggtttcggca
     8701 ttcgcgacct cggcttgttc cagtcggcga tggcgcgccc gctcaacctc gccgcctatg
     8761 gcgaaccgga cctcgccgcg ttggcttcgg cttacgccta tggcattgcc cgcaatcacc
     8821 ccttcatcga cggcaacaag cggacggcgg cggtggtcgg cgaaaccttc attctgctca
     8881 acggaggccg gctggaggtc gatgatgccg aactggccgt ggtcttcctc gccctcgccg
     8941 ccggcgagct ttcggaggag gcgctggcgg agtggtttcg ggagcggatt atcgcgtcgc
     9001 aagagtgact tggtgactca ttctttaccc tgttgcggct atggctccag gtcatgcgga
     9061 gcgggtcgtg atcatcagcc ggggcaacgg caatgtcgcc gaaaaggccg agcgtccggc
     9121 gccgccgcct aagccgcgcg ttcccgccgc ggagctcccg gcaaagccgg tcgagacgcc
     9181 ggtgcccgcc gccgatttcc ggctcgtcca ccaccgcctg gcggcgctcg agcggctcgc
     9241 cgccctgctt gaaaagggcg cgctcgacgc agccgagttc gccgccgaaa aggcgctgat
     9301 cctgcgcttg ccggcagagg aactggtgct gggccccgac agggcggcac ggccgccgcg
     9361 cgggccgtcg ctgctcggcc gcctgctcgg ctggcgcgtg ctcgccgccg gcgccgcgct
     9421 gggcctcgcg gtctcgttcg cgacccagcc gcaggaactg ctcaccgtcg ccgagcgcgc
     9481 cggccggttg ctcggctgag ctaggccgcc gagcaggcgc ggcaggcggg atccttgccg
     9541 acgcgcagcg tccggaggcc gggcttgagc ccatcgaaga tgtggatctt gccggccgct
     9601 tcctcgccga aaccgacgat ctgccggacc gcctccagcg ccgcccagct tccggcgatg
     9661 cccgccatgg cgccgagcac gccaagctcc gagcaggtgt cgcaatcctc cgcatcgaac
     9721 gcgtcgccga cgaagcagcg atagcagggc cggcccgcct cccagccccg ccaggtgccg
     9781 acctgcgcct ggaactggcc gatggcggcg gacacgagcg ggatccgcat cgcggtgcag
     9841 gcgtcggaga cggccaagcg ggtggcgaaa ttgtcgcttc cgtcgatcac gacatcgctc
     9901 ccgtcgatca gccgcgcggc atcggccgaa gacaggcgct cgcgcacgcc ttcgaacgcg
     9961 acgcccggat tgagtcgcgc caccgcttcg ccggagactt cgaccttgcc gcggccgaca
    10021 tcgccggttc cgaacagcac ctgacgctgg aggttatcca gcgtcacgga atcgtcgtcg
    10081 atcaccctca gccgtcccac gcccgccgcg gcgagatact ggatcgccgg cgagccgatc
    10141 ccgcccgcgc cgatcacggt aacgcttgcc gccagcagct tccgctgtcc ctcgccccct
    10201 atctcccgga ggacgatgtg gctggcgtaa cggtcgagct ggtcgtcgct gagcgtcacc
    10261 gccccgtcga gccgaagccg ccggcgccgc gttcggtcgc gtccagattc tcgacctcgg
    10321 cgaagatcgc ccgctggacc ggcgccggca ccagctgtgc gatccgctcg ccgcggacga
    10381 tctcgaacgg ctccgcgccg agattggcga ggatcacctt cacctcgccg cgataatcgg
    10441 cgtcgatcgt gccgggcgtg ttgaggcagg tgatgccgtg cttcagcgcc agcccggagc
    10501 gggggcggac ctgcacttca taaccctcgg ggatggcgac ggcgaacccg gtcgccaccg
    10561 catgccgcgc tccgggggcg agggtgaggc tttcggccgc gaccacgtca aggccggccg
    10621 catgcgcggt cgcgtagcag gggaggggca ggtcagcccc atgcggcagc cgcttgagcg
    10681 agatgcggat ttcactgaag ggcatcggcg atcctgtcgg cgaggtgccg ggcaacgtcc
    10741 tgcttggggg ccttgtccca gctctcgacg ccgtcggcgg tgatcagatg aacggtattt
    10801 tcatcggcgc ccatgacgcc tttggaaaca tcgttggcga cgatccagtc gcagcccttg
    10861 cggtggcgct tcgcgcgggc ggcctcgatc accgcatcgg tctgggcggc gaacccgacc
    10921 agcaggcgcg ggcggtgccg gctctcggcg agcccgagta ggatgtcggg gttggcggac
    10981 caggcaagct cgggcggccc gtccgccttg ctgagcttcg acggcgccgg ctcgacccgc
    11041 cagtcggcca ccgccgcgac tagtaccgcg gcatcggcgg gaagcgccgc ccgcacggcc
    11101 gcgtccatct gccgcgccgt ctcgacgtct acccgttcca cgcctggcgg cgtcgcgagg
    11161 ctcaccggcc cggccactag cgtcacccgc gcgcccagct cggccagtgc ttcggcgatc
    11221 gcgaagccct gcttgcccga cgaacggttg gcgatcaccc gcaccggatc gatcggctcg
    11281 tgcgtcgggc cggccgtgac gaggacatgc cttcccgcta ggcttcctgc cgtgtgcggc
    11341 gcgcctgagc ttttctgccc ggaaagctgt tcacgtatcg ctgcgaggat ccgctcaggc
    11401 tccggaagcc tgccgggccc atattccccg cacgccatct cgccgtcgtc cggctccaac
    11461 accgtcacgc cgtcgcgcct gagctgctcg acgttacgcc gcgtcgcctc gtgccgccac
    11521 atccgcacgt tcatcgccgg cgcggccagc accggcttgt cggtggcgag caggagggtg
    11581 gtggcgaggt cgtccgcgat gccggccgcc atcttcgcca tcaggtccgc cgtggcgggg
    11641 cagacgacga ccaggtccgc ctgacggctg agctggatat gccccatctc gacctcgtcc
    11701 ttgaggtccc aaaggctcgt atagaccttg ttctcggaca gcgccccgag cgtcatcgcg
    11761 gtgatgaaat gcgatccgcc ctcggtcagg acgcaggtca cctcgaggcc gtccttgcgg
    11821 agcagccgga tcagctccgc gctcttatag gctgcgatcc cgccgccgac gatgaggagg
    11881 atgcgcttgc tgttcaactt ggtctcagtc ctgcttgcag cttcggcacg gccgcgcgta
    11941 ccattgcttg atggaactca acaacgaaca ggactgaagc ctagggagaa ggagggtcga
    12001 atgcgacgca ttttgtgttc catgttggct ctttcaatgg cggcctgcgg cactgtgtac
    12061 ggactcggcg aggacgttca gtccgctggc gaggtcatag aggtgccggc caaccccgca
    12121 cctccttcac ctgcgccccc cgtaccggca ccgccgcttc cacctccacc accaactaac
    12181 ggcgatcatg acgaatcggc agagatccgc actacgggtg tcggcgttac tggatcggct
    12241 ccatcgggca gcagtcgcgg ccctggtggc cgaccagagc ggcaggaggg ggcagtcagg
    12301 cggatagatc cagccgctga accggcgaca cgagtggtgc cagacactac agctcgcgct
    12361 tcggccccac aaccatgctc gctcgaggat cctcgctgcg agggcaagcg agggttaggt
    12421 ggcttcgtga agccagggga tatgtggctc ggcaccccct caagcctgca gtacgtcgtt
    12481 gcccccgatg aggaggcaat cagtttagag accgaggggg cgaacatggc atctgtccgc
    12541 ccgatcgcca tcgggcgtga aatgcgcgtg acgctccttc cacattggaa cttcgagttg
    12601 ctcagcccca atggggagaa gaagcccttg ggactggacg aatccgctac ctggcaatgg
    12661 caggttcgac cgaaggagca gggaggaccc tttgagatac tggccgaagt ccaagttttc
    12721 ggacctgacc gtgacggcaa agctactgtg ctcgacacgt ataccaggcg cgtggccgtg
    12781 caggtgcatg tcggcacttg gaatggcttc ctcaatgccc ttgcgaaggc caagacgctt
    12841 ggggagttgt tccaggcctt attcgcgact tggaagtcca cccttatttc tttgtcggcc
    12901 ttgctggccg ccttgggcgg cgtgatcgtg gcagtgcgaa ccttgggctc aaaaaagggt
    12961 caggaaacac gagaacagac gccagcaccc gactaaacga aaagctgagc gagcagagct
    13021 cccacggcag cggcgatcag tgccgtcagg aaatagctgc catatcgccg cggccgcacc
    13081 accaccacat cgggaagcgg cggcggggga ggggcggcgc cgggcggcgg gtaataatga
    13141 tcgagccggc ggatcaggtc ggggagcccg gccagggtcc ggaaatcgcg caccagccgg
    13201 tcggccagcg ccgcttccgg cccaagctcg ctccgcagcc attcgcgcag gaacggctcg
    13261 ctcgtctccc acatgttgat gtccggatcg agcgcggtcg cgacgccttc ctccatcacc
    13321 atcgtcttct ggagcagcag caggtgcggc tgggtcggca tgtcgaaatc gcgcgtgatc
    13381 gtgaacaggc tgtcgagcat ctgcgccacc gaaatgtcct tgaccggcag cccgcggatc
    13441 ggctcgcccg ccgcccggag ggcggtcgcg aactcctcga cgctatggtg agacggcaca
    13501 tattgcgcct cgaaatggat ttcggcgacg cggcggtaat tgccgacgat gaggccgtag
    13561 aggatttccg ccagccacat ccgcgcttgg cgatcgactc gtcccatgat cccgaaatcg
    13621 atcgcggcga ggcggccgtc ggggagggcg aacagattgc cctggtgaag gtcggcgtgg
    13681 aagaagccgt caatcaccgc ctggcgcagg aaggcgcgga ccaggatcgc cgccagcgcc
    13741 ttgaggtcgt gcccggccgc gaccagcgcg tcgcggtcgg tgagcttgat cccgtcgatc
    13801 cattccagcg tcatcacgcg gcgcgaggtg cgcgaccagt cgatcgccgg gacgaagaag
    13861 ccgctttcgg cgaccaaatt ctcgcgcagc tcggaagcgg aggcggcctc gcggcggagg
    13921 tcgagctcgc gcgcggtcca ttgcctgaaa tgcgcgatca ccatgcgcgg ccgtagccgg
    13981 gccgcttcgc cgcccatgct ctcgacctgg gcggcggccc attcgtacgt gtccatggcc
    14041 ctggcgaaat catcctcgat gccgggacgc aggaccttga ccgccacctg ctggccatcg
    14101 gtcgtaaccg cgcggtggac ctgggcgatc gaggcggcgc cgaccggctc cgggtcgata
    14161 tgcgagaagg cctggtcgat cggcttttcg agcccttgct cgatgacccg gcggatctgt
    14221 tcgaacggcg cgggcggcag gctgtcctgg agcagcgaaa ggttgcgcgc ggcttcgtcg
    14281 ccgacgaggt cgggccgcgt cgccagcgcc tgcccgaact tgatcgcggc cgggccgatc
    14341 tcctggaaag cctcggcata agtggggctg gcgggcaccc gcgcgccgaa acgggcgatg
    14401 cgggcgagcc ggcgcaccgg cggcggagtg ttgggatcgc gttcgatccc gcgcagcgca
    14461 ccgtgccggg cgagcacgcg gccccatttc aggagccgcc agatatggac ggcgggtgcc
    14521 gtcatgcgga tgtgccccgg cgaaggccgg ggcccagagt ctcttgttcg gctggatccc
    14581 ggccttcgcc gggacgcgat gcgcagcagc gcatcatatc ttccagccgc tgtggatggc
    14641 gacgaggccg ccgaggatcg gctcgaccga gacgcgcgcg aagcccgctt gctcgatcat
    14701 cgccttgaag gccggcatgt tcgtgaagcg ccggatcgat tcgaccagat agcggtagct
    14761 gtcggcatcg tccgccaccg ccttgccgat ccgcggcacc acctcgtggc tgtagcggtc
    14821 gtagagctgg gcgaagccgg gccagctggt ggtcgagaat tcgaggcaga agaaacggcc
    14881 gccgatccgc agcacgcgat gcgcttcggc cagcgctgcg ccgatgtcgg tgacgttgcg
    14941 gatgccgaag gcgatcgtat aggcgtcgaa cgtctcgtcg gggaagctca gcgcctcggc
    15001 attctcctcc gcccatagca gccccgggac gccttgcctt cgggaccgct cgatgccgac
    15061 ggcgagcatg tcggggttga tgtcggcgac cgtcacctgc gcgcccgacg cggcgatccg
    15121 gaaggcgacg tcgccggtgc cgcccgccat gtcgaggatc gtctcgcgcg cccgcggccg
    15181 caggcgccgg acgaactggt tcttccagtg ccggtgcagg ccgcccgaca tgaggtcgtt
    15241 catcaggtcg tagcggcgcg cgacgttgga aaagacccgg ccgacgcgct gcgtcttctc
    15301 ctcgggcggc acgtcctcat agccgaaaga aaccttgtcc atgggcgctg cctctagcag
    15361 gggtggggcc gagatgaaca ggacgaacca tgcctgaatt gccggaggtg gaaacgacgg
    15421 tgcggggact ggttcccgtg ctggaggggc ggcggctcgc gtcggtcgag acgcggcggc
    15481 cggacctcag gcggccattc ccgcctgacc ttcgccagcg gctcaccaat gcgaccgtca
    15541 ccggcctcgg ccggcgcgcc aaatacgggc tgatcgggac cgaccgcggc gacaccctga
    15601 tcttccatct cggcatgtcc gggcgctggc ggatcgaccc ggccgagctg ggcccgcacg
    15661 atcatgtgct gatcgcgacg gaggagggga ggcggctggc gctcaacgac ccgcgccggt
    15721 tcggctcgct ggacctcgtc ccgaccgcgg acctcggcgc ctacccgccc ttcgtggcga
    15781 tggggccgga gccgctcggc gaggctttct ctccggccta tctcgccggg gcgctcaagg
    15841 ggcggagcgc gccgatcaag gcgctgctgc tcgatcagag gatcgtcgcg gggctcggca
    15901 acatctatgt ctgcgaggcg ctgcacatgg ccggcatcgc gcccggccgg gccggcgggc
    15961 ggatcggcgg cgagcggctg gcccggctgg tggaggccgt caagacggtg ctgctcgccg
    16021 cgatcgaggc ggggggatcg tcgctgcgcg actatgtccg gcccgatggc gaactcggct
    16081 atttttcgaa gcaatggcgg gtctatgggc gcgagggcga aagctgcggc tgcggcgtcc
    16141 cgattcgccg ccgcgccgag ggcggccgct cgacattcta ttgcgccgcc tgccagcgct
    16201 agaggttgac cgtcgccggc tctttgcgta gagagccgcc ctgaccggtg cgcctgatcg
    16261 cgcgccgttt tcatttcacc gatacgaggc aagacgatgg cgaatacgcc gcaagcaaag
    16321 aaacgcatcc gccgcaacga tcgccgggcg gaaatcaacg gcgcccgcgt cagccggatc
    16381 cgcacattcg tgaagcagct tgaggccgct ctggcgaccg gcgacaagga ccaggctacc
    16441 gccgcgttca gccgcgtcca gcccgagctt gcccgcggcg tcgccaaggg cgtggtccac
    16501 aagaacaccg cctcccgcaa gatttcgcgg ctgtcgaagc gggttgccgc gctcggctga
    16561 ccagccgcgc gaactccggt ttttcaaggc ccgccggcac ctcgccggcg ggctttttca
    16621 tgcccggacg ccgattgccg gtaagtccag catttccggc gattcgcgga catcgttgca
    16681 ctaatgttac aatcttaaaa tgttggaaaa gcaggctatt tcgccagtag cacgggattc
    16741 cgcgcctttc gcctgtcaac gcatttattt cacataaatt acattttcag cacttgatcg
    16801 ggctcatgcc gagtccctaa aaccgaatct ccggcgggcg aatcttcgtc ggatacaaac
    16861 agcataacga tgttcagcaa cggcgtcgcc ggggggtgtt tcgccgcgcg ggggagagtt
    16921 ttgctcgata agaagccgta aaaagcggca gaggttttga gggggtgcgt gcgtgaacca
    16981 ttcgccggcc aagggcgtag ctttgccagc taacgaaggt gtcgccgagc cgatcgcggc
    17041 ggcctgggag cagatccgcg ccggccttcg ccgcgactgc ggtgcccgta ccttcgacgg
    17101 ctggctgcgc ccgatcgcgc tggccggctt cgatcccgac agcgccaccg tccggctcga
    17161 gcttccctcc caattcatgg ccgactgggt ccagacccat ttcgccgagc ggctcggcct
    17221 tgcctggcgc acgatgctgc cgctggtccg cgacgtcgtg atcggggtcg gtgccggggc
    17281 tggccagcgc accgtgaccg ccctcgaaat cccgccgccc ccggccgagc ccgtctcgcc
    17341 cgccgagcag cccggcgggc tgtacgcggc gcagctcgag ccgcgctacc gcttcgagac
    17401 cttcgtcgtc ggcaaggcca acgaggtcgc gttcaacgcc gcccgcaccc tcgccgccgc
    17461 cgacaatgtc gcgttcaacc cgcttttcct ccacggcggc accggccgcg gcaagaccca
    17521 cctgctgcac gcgatcggac acgaatttcg cgccc
//
LOCUS       DASVRK010000283        11072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_432874, whole genome shotgun sequence.
ACCESSION   DASVRK010000283 DASVRK010000000
VERSION     DASVRK010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432874"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1128
                     /locus_tag="VF589_11590"
     CDS             238..1128
                     /locus_tag="VF589_11590"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX8258266.1"
                     /translation="MTDQPNYDDRDGWIWFDGALVPWREANVHVLTHALHYASSVFEG
                     QKAYGGEIFKLSEHSARLRRSAELLGFELPWTVEQIDQACREVLEANGLVDAYMRPLA
                     WRGSEQMGVSAQRAKIHLAISAWPWGAYFGEEAVRKGLRLDIAPWRRPAPYTAPTGSK
                     AAGLYMICTLSRHQAQAKGYDDALMFDWRGRVAEATGANAFFVRDGALHTPVPDCFLD
                     GITRRTVMDLARRRGIEVVERPIWPEELESFEQFFLTGSAAEVTPVAEAGPWRFEVGE
                     LSLQLRKDYLDLVNRRLPNA"
     gene            1247..1321
                     /locus_tag="VF589_11595"
     tRNA            1247..1321
                     /locus_tag="VF589_11595"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1279..1281,aa:Gln,seq:ttg)
     gene            1539..2930
                     /locus_tag="VF589_11600"
     CDS             1539..2930
                     /locus_tag="VF589_11600"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8258267.1"
                     /translation="MPAASARATEPASRFGFRLTATLLVVCVAFGGRSAGTPLSEALM
                     RTLAVGLLLWLAFAPRRAADSKLIIASVLLMLATLLAGALQMLPLPEGMWSSLSGGPI
                     AADLVRTSLGEVGWRPLTLNIDGTRETLLTLLPPFALFLAVIEMDAADRRRLAFLVLI
                     LAALSVVVGLLQFASRTGMYPYETTHQGHSVGLFSNRNHQADLTLIAALLAAVFFAGR
                     KGGQAGAAGTLVAVALLAVIALNLAAAASRTGIMLAIPAFLLGGWIAFGKRARSKWTL
                     VAAGALVVIAVTSVMMRPDLFAHVSDRFASTTDDTRFEAWPEVWKVIKLYMPLGSGLG
                     TFVPVHQSIENLDMVSDRYLNHAHNDYLEILLEMGVVGVLLLVWFFVLLLWAVKDNLL
                     APAVNERYRALSLAAAGGIAILLAHSVVDYSLRTSTLMAVFALFFAILMPLPEREPGR
                     PRQGRRGAAADEE"
     gene            3032..3739
                     /locus_tag="VF589_11605"
     CDS             3032..3739
                     /locus_tag="VF589_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX8258268.1"
                     /translation="MIRAFVFTCLSGSLLLAGCASTAPVVPSGAAAYQNFPALTTAPT
                     LAEYKIGPYDVISISTYQEEGLSRENMKVDASGNIILPLIGPVQAAGHSAEDLSRIIA
                     TRFGERYLQNPQVTVVVNSAASQRVIVEGAVRDAGVYPIEGGTTLLQAIALASGTTEV
                     ASTVDVVIFRTINGQRMAGAFSLKDIRRGISEDPQIKGNDVVVVGLSAGKQLYRDILA
                     ASPLVAGIFRPIATSAN"
     gene            3781..6069
                     /locus_tag="VF589_11610"
     CDS             3781..6069
                     /locus_tag="VF589_11610"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX8258269.1"
                     /translation="MRPTFSLRADQGGAVPIEPTPLEGPAESFADGGGGGGGGLHLDF
                     RRIWAGLYRNRWLVGGILVAAIILGVIATALTVPLYRATTRVEIERQSANVLNVEDVE
                     QEETSQDVGQFLQTQVQILRSRSLREAVADDLGLIRDNSFLEKMNGPLPDEALRPEQQ
                     RGARRDFVLDVLEDNLNVVLLPSSRIVELSFSSPDKTLSSRVANSFAKKYIEGNLSRR
                     FETSSYARTFLNDQLGKAKERLEASERSLNAYARQAGLIDTGATGATTGGDSGTSLTT
                     TSLTQFSTALNAARTQRIGAEQKWNEARGAPALSIAEVYRNPAVSALVQRRAQLRADY
                     EEARQRRKEDFPEMRQMAAEVEQIDRELNSMASGIRNSIRRDYEIARAQETALEQRVA
                     GLTSDRLGEQDRSVQFNILNREVQTNRVMYDGLLQRFKELSAAAGLAANNISVVDKAE
                     PPRFPYKPQPVLNIALATFMGLVLGILAALARERLAGRLRVPEDIENNLGVPVLSTIP
                     VVQKADLSETLKNPRSPASEAYHTLRTALELSTSTGVPKTLLFTSGQQGEGKSTSALT
                     VATDFAKGGRKVLLVDADMRRPSLHRRLDRFNETGLSSILAAQAALPSAVQEIAGHPN
                     LSFLSSGPLPPSPAELLSGPMLRSFLDQASAAYDLVILDGPPVLALADATILASKAAG
                     TVMVIEADRSRVQQVRSSIERLRAGGARLIGAVFSKFDARHMGYEYDYRYNYEYRESE
                     DGDGGGGGGEGVAGKARRFLGR"
     gene            6069..7295
                     /locus_tag="VF589_11615"
     CDS             6069..7295
                     /locus_tag="VF589_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258270.1"
                     /translation="MTERRPRWLVILLILVGVAMAVVLMMQGVGRWAYARGIGGLLEI
                     SGSQDADVYSKNGQRYFEQHQYKVAEEFARAALNRAPLHQKAMRTLGMAQVAQGREEQ
                     GRRTMEAAGRLGWQDVATQTWLMWDSLQRGNTQAGLLRADALARRGTMASGLFKVFIT
                     FASAPESRRMLMQRLQIASGWRPDFFRFSDAATLPEAQTAEGLLRDLRKGPWPPAREE
                     VVPIVRRLVVLGAYDRAANLSRDMLGEPRTNASNPVGDGDFKRFDVYAERPLYRTPFD
                     WEFGVHPGATAVVERPDSPEDGALYVESNGAKTVMLARKVVRIAPGARQLAFTIRSSD
                     AGAIERFRWSVTCLETGQQLIQGAMLAPATNVASQRGLAFTVPADCRFQQLQLSSAEA
                     PQRQTLSAYLDNVAIS"
     gene            7316..8071
                     /locus_tag="VF589_11620"
     CDS             7316..8071
                     /locus_tag="VF589_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8258271.1"
                     /translation="MTEIGIAPKVARIYPEIAAGGFSHVDGTIGFYQRINALIGPDME
                     VLDFGAGRGEQLVEDPVPYRAALCRLQGKVRRLVGVDIDPAVLRNPFLDEAHVIDPSR
                     PLPFADGQFDLIYADWVLEHVATPDGFAAEVRRLLKPGGWFCARTPNRWGVTGLGANL
                     IPNRLHTKLLKTLQPGRQEQDVFPTTYKMNTRSAVRRYFPEREWEHSTYISNSEPPYV
                     QRSLLLMRLVQLFWRASPPGFHTTLNVFLRKRA"
     gene            complement(8171..8926)
                     /locus_tag="VF589_11625"
     CDS             complement(8171..8926)
                     /locus_tag="VF589_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8258272.1"
                     /translation="MNDVQSNPSPRFRPMWLWLLLTAAAVPCLAAGALIAAMTTQRGE
                     PIPNRVHVLGDSYAASRLFQQALREALPGYDISFDAVGGSSLAEQLERLRGGGRDMGG
                     AEGGGRELWSASLVIVDGGLTDRDPLVSVEGIAATLTSECPTWFYVEPPHRAVPNAPI
                     GSPRYEQQEAAVQAIRAKYPKHFIPTKEALVTRVPARGPGDEQDRANGWVPRSLRADR
                     VHLNAEGYDIMARLIAERFRAAQARRLAEPCGL"
     gene            9356..10852
                     /locus_tag="VF589_11630"
     CDS             9356..10852
                     /locus_tag="VF589_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HEX8258273.1"
                     /translation="MRPSATLLSQAAPYISVVGIGGSILVAAIALPIGILLDSSGMVY
                     LSTAFLAFAIPAAVGVARGRNFDPFEPINLVALAIFFGTSLRAVYLLTSDTERAAFVM
                     MGTDFENVAANAPWMLLSVAALVLGYLITPWRIPIEKFRAVRCYAINRTRLNVAIGLC
                     VIITLVAIAMLAAREGLDFSQGILSLSRKRAAVYTSDEGAVVYGVGYEAFLGQFAQYA
                     FVLLSCVVLSGIVKQRLGTLVALVLLGVLGCVTPFLVSSRSTIIIMLLSAAIAATYYG
                     KLKAKVLVVIFLTSIPLVVGMGYLRQVNHARGAVERAEGTSVMDSIIGSGNSLDFVRT
                     SAIMDRIPSVRGYQWGWTYAALFSAPIPRAIWPEKPNPSLGPWVKRELYGKPVRNQGW
                     PSGMIAEGYINFGTVGLIIPLFLFGALLRTLYETCRPLLGVSLPVTAIYAVSIWRLGF
                     GTIGLNFAHGMMQMLTDLAPMFLLVVIARAPLRKAIADRVVGARRLPV"
BASE COUNT         1684 a         3815 c         3681 g         1892 t
ORIGIN      
        1 gcagccggtg cggcgggcga gctccaccgc cgcccgctcg aggccgcgcc cggcttcggc
       61 ggcggccacg gcccgatggg ccacgcgctc ggagtggtgc cgttcgacat cgtgtcggca
      121 ggcggccgat cgtccggcgc cgggccgcgc acccgctgac gcgcggcccc gacatttgac
      181 cgaccgcgcg gtcagcctta tccccgctca accacttcaa ggggactcga acgccagatg
      241 accgaccagc ccaattatga cgaccgcgac ggctggatct ggttcgacgg cgctttggtg
      301 ccgtggcgcg aagccaacgt ccacgtgctc acccacgcgc tccattacgc ttcctcggtg
      361 ttcgaggggc agaaggctta tggcggcgag atcttcaagc tgtccgagca cagcgcccgc
      421 ctccgccgca gcgccgaatt gctcggtttc gaactgccct ggaccgtcga gcagatcgac
      481 caggcctgcc gcgaggtgct cgaagccaac gggctggtgg acgcctatat gcgcccgctc
      541 gcctggcgcg gctccgagca gatgggcgtg tcggcgcagc gtgccaagat ccatctcgcc
      601 atttcggcct ggccgtgggg cgcctatttc ggcgaggagg cggtgcgcaa ggggctccgg
      661 ctcgacatcg cgccgtggcg ccgtcccgcc ccctataccg cgccgaccgg ctccaaggcc
      721 gccggcctct acatgatctg cacgctgtcg cgtcaccagg cgcaggcgaa gggctatgac
      781 gacgccttga tgttcgactg gcgcggccgc gtcgccgagg caaccggcgc caacgccttc
      841 ttcgtgcgcg acggcgcgct tcatacgccg gtgcccgatt gcttcctcga cggcatcacc
      901 cgccgcaccg tgatggacct cgcccgccgc cgcggcatcg aggtggtcga gcggccgatc
      961 tggccggagg agctcgaaag cttcgagcaa ttcttcctca ccggcagcgc ggcggaggtg
     1021 acgccggtgg ccgaagccgg gccgtggcgg ttcgaggtcg gcgagctcag cctgcagctg
     1081 cgcaaggatt atctcgatct ggtcaaccgg cgcctgccca acgcctgagg ccgcagggga
     1141 catccggtgc ccgcgcggcg gcggcgatga tttcgggccc ggcggcacgg ccttgctgga
     1201 cgcgcccgcc gaggggcact aaagcccggc gcgccggctc ggatgctggg gcgtcgccaa
     1261 gtggtaaggc accggttttt ggtaccggca ttcgcaggtt cgaatcctgc cgccccagcc
     1321 aggccgcggt tttggcggga ggcgcgcgag cgagcgcgct tgattcgggc aaagttgcaa
     1381 tatagtcagc cgtctagtgg ggggtgcgga attgagctcg tcgaacgggc cccgccgcgc
     1441 tcgcaaccca gttcgcgccc cgcggcgcgg cgggcgtctg ggacaggctc taggtggaag
     1501 gttcggcgac taagccaatg acaagtcagg tgcgttccat gcccgcggcc agtgcgcgcg
     1561 cgaccgagcc ggcctcgcgc ttcggctttc ggctgaccgc gaccctgctg gtcgtctgcg
     1621 tcgccttcgg cggccgctcg gccggcacgc ccttgtcgga agcgctgatg cgcacgctgg
     1681 cggtggggct gctgctgtgg ctcgccttcg cgccgcgccg cgccgccgat tcgaaactca
     1741 tcatcgcttc ggtgctgctg atgctcgcca ccttgctggc cggggcgctg cagatgctgc
     1801 cgctgcccga gggcatgtgg tcgtcgctgt cgggcgggcc gatcgcggcc gacctcgtgc
     1861 gcacgtcgct gggggaggtg ggctggcgcc cgctgacgct caatatcgac ggcacgcgcg
     1921 agacgctgct gaccctgctg ccgcccttcg ctttgttcct cgccgtgatc gagatggatg
     1981 ccgccgaccg ccgccggctc gcctttttag tgttgatcct ggccgcgctc tccgtcgtgg
     2041 tcggcctgct gcagttcgcc agccgcaccg gcatgtatcc ttacgaaacc acccaccagg
     2101 gccattcggt ggggctgttc tcgaaccgca accaccaggc cgacctcacc ctcatcgccg
     2161 ccttgctcgc cgccgtcttc ttcgccgggc gcaagggcgg gcaggcgggc gcggccggca
     2221 ccctggtcgc cgtcgccctg ctcgcggtga tcgcgctcaa cctcgccgcg gccgcctcgc
     2281 gcaccgggat catgctcgcc atccccgctt tcctgctcgg cggctggatc gcgttcggga
     2341 agcgggcgcg gagcaaatgg accctggtcg ccgccggcgc tttggtcgtg atcgccgtca
     2401 cctcggtgat gatgcggccc gatctgttcg cccatgtctc cgaccgtttc gcctccacca
     2461 ccgacgacac ccgcttcgag gcctggcccg aggtctggaa ggtgatcaag ctctacatgc
     2521 cgctgggctc cgggctcggc acgttcgttc cggttcacca gtcgatcgag aatcttgaca
     2581 tggtcagcga ccgctacctc aaccacgccc ataacgacta tctcgagatc ctgctcgaaa
     2641 tgggcgtggt cggggtgctg ctgctggtct ggttcttcgt gctgctgctg tgggcggtga
     2701 aggacaacct gctcgcgccc gccgtcaatg aacgctaccg cgcgctgtcg ctcgccgcgg
     2761 cgggcgggat cgccatcctg ctcgcccatt cggtggtcga ttattcgctg cggacatcaa
     2821 ccctgatggc ggtgttcgcg ctgtttttcg cgatcctgat gccgctgccc gagcgcgagc
     2881 cgggccggcc ccggcaagga cggcgcggcg cggcggcgga cgaggaatga gcggcagcgg
     2941 gccgggagcg gccggcacgg gagcaaagcg gtcgtgcgcg cttaccgcgc cggctctttt
     3001 ctcgcataat atacgggttt agagaggtag tatgattcga gcgtttgtat tcacgtgcct
     3061 gtcgggctcg ctgctgctgg ccggctgcgc gagcactgcc cccgtcgtcc cgagcggggc
     3121 ggcggcctat cagaacttcc cggccctgac gacggcgccg accctcgcgg agtacaagat
     3181 cggcccctat gacgtgatca gcatctcgac ctatcaggaa gaggggctga gccgcgagaa
     3241 tatgaaggtc gatgccagcg gcaacatcat cctgccgctg atcggcccgg tccaggccgc
     3301 cggccacagc gcggaggact tgtcgcggat catcgccacg cggttcggcg agcgctacct
     3361 ccagaacccg caggtcaccg tggtggtcaa tagcgccgcc tcgcagcgcg tgatcgtcga
     3421 aggcgcggtg cgcgacgccg gcgtctaccc gatcgagggc ggcacgactt tgcttcaggc
     3481 gatcgcgctg gccagcggca cgaccgaagt ggcctcgacc gtagacgtgg tcatcttccg
     3541 caccatcaac ggccagcgta tggccggcgc cttcagcctc aaggacatcc gccgcgggat
     3601 ctccgaggat ccccagatca agggcaacga cgtcgtcgtc gtcggcctgt ccgcgggcaa
     3661 gcagctctat cgcgacatcc ttgccgcttc gccgctcgtg gccggaatct tccggcctat
     3721 cgcgactagt gcgaattaaa gcgtggccgg agcgcaccag aaagaagtgg tagtaacagg
     3781 atgagaccga cattcagctt gcgtgccgac cagggcggcg cagtgccgat cgagccgacc
     3841 ccgctggaag ggccggcgga aagcttcgcc gacggcggcg gcggcggcgg cggcggactt
     3901 cacctcgact tccgccggat ctgggcaggc ctctaccgca atcggtggct ggtcggcggc
     3961 atcctcgtcg cggccatcat cctcggtgtg atcgcgaccg cgctcaccgt tccgctctac
     4021 cgggccacga cccgcgtcga gatcgagcgc cagtcggcca acgtcctcaa tgtcgaggat
     4081 gtcgagcagg aagagacctc ccaggatgtc ggacagttcc tccagaccca ggtccagatc
     4141 ctgcgcagcc ggtcgctgcg cgaggcggtg gccgacgatc tcggcctgat ccgcgacaac
     4201 agcttcctcg agaagatgaa cggcccgctc ccggacgagg cgctccgccc cgagcagcag
     4261 cgcggcgccc gccgcgactt cgtgctcgac gtgctcgagg acaatctcaa cgtcgtgctg
     4321 ctgccctcgt cgcgcatcgt cgagctcagc ttctccagcc ccgacaagac gctttcgtcg
     4381 cgggtcgcga acagcttcgc caagaaatat atcgagggca atctcagccg gcggttcgag
     4441 accagtagct atgcccgcac cttcctcaac gaccagctcg gcaaggccaa ggagcggctc
     4501 gaagcgtccg aacggtcgct caacgcctat gcccgccagg ccggcctgat cgacaccggc
     4561 gccaccggcg ccacgaccgg cggcgattcc ggcacctcgc tcaccaccac cagcctcacc
     4621 cagttcagca ccgcgctcaa cgccgcccgc acccagcgga tcggcgccga gcagaagtgg
     4681 aacgaggcgc gcggcgcccc cgcgctcagc atcgccgaag tctatcgcaa tcccgcggtc
     4741 agcgcgctgg tccagcgccg cgcccagctg cgcgccgatt atgaagaggc ccgccagcgc
     4801 cgcaaggaag acttccccga aatgcgccag atggccgccg aggtcgagca gatcgaccgc
     4861 gagctgaact cgatggcgtc gggcatccgc aactcgatcc gccgcgacta tgagatcgcg
     4921 cgggcacagg aaacggcgct ggagcagcgc gtcgccggcc tcacctccga ccggctcggc
     4981 gagcaggacc gcagcgtcca gttcaacatc ctcaaccgcg aggtgcagac caaccgcgtc
     5041 atgtatgacg gcctgctcca gcgcttcaag gagctcagcg ccgccgccgg ccttgccgcc
     5101 aacaacatct cggtggtcga caaggccgaa ccgccgcgct tcccctacaa gccgcagccg
     5161 gtgctcaaca tcgcgctcgc gaccttcatg gggctggtgc tcggcattct ggccgcgctc
     5221 gcccgcgagc ggctcgccgg acggctgagg gtgccggagg atatcgagaa caatctcggc
     5281 gtccccgtcc tctcgaccat cccggtcgtc cagaaggcgg acctttccga gacgctcaag
     5341 aacccgcgct cgcccgcgtc cgaagcctat catacgcttc gcaccgcgct cgagctcagc
     5401 acttcgaccg gcgttccgaa aaccctgctg ttcaccagcg gccagcaggg cgagggcaag
     5461 tcgaccagcg cgctcaccgt cgcgaccgac ttcgccaagg gcggccgcaa ggtgctgctg
     5521 gtcgacgccg acatgcgccg cccgtcgctg caccgccggc tcgaccggtt caacgagacc
     5581 ggcctctcca gcatcctcgc cgcgcaggcc gctttgccga gcgcggtcca ggagattgcc
     5641 ggccatccga acctgagctt cctgtcgagc ggtccgctgc cgccgagccc ggcggaactg
     5701 ctgtcggggc cgatgctgcg ctcgttcctc gatcaggcca gcgccgccta cgacctcgtc
     5761 atcctcgacg ggccgccggt gctggcgctc gccgacgcga ccatcctcgc ctccaaggcg
     5821 gccggcaccg tgatggtgat cgaggccgac cggtcgcggg tccagcaggt gcgctcgtcg
     5881 atcgaacggc tccgtgccgg cggcgcacgg ctgatcggcg cggtattctc caagttcgac
     5941 gcccgccaca tgggctacga atatgactat cgctacaatt acgaatatcg cgagagcgag
     6001 gacggcgatg gcggcggcgg cggcggcgag ggcgtggccg gcaaggctcg ccgcttcctc
     6061 ggccggtgat gaccgagcgt cggcccaggt ggctggtcat tctcctgatc ctggtcggcg
     6121 tcgccatggc cgtcgtcctg atgatgcagg gcgtcggccg ctgggcctat gcgcgcggca
     6181 tcggcggcct gctcgaaata tcgggatcgc aggatgccga cgtctattcc aagaacggcc
     6241 agcgctattt cgagcagcac caatataagg tcgccgagga attcgcccgc gccgcgctga
     6301 accgcgcgcc tttgcatcag aaggcgatgc ggaccctggg catggcccag gtcgcgcagg
     6361 ggcgggagga gcaggggcgg cgaaccatgg aagcggccgg ccggctcggc tggcaggatg
     6421 tcgccaccca gacctggctg atgtgggatt cgctccagcg cggcaacacc caggcggggc
     6481 tgctgcgcgc cgacgcgctc gcccggcgcg ggacgatggc gtccggcctg ttcaaggtgt
     6541 tcatcacctt cgccagcgcg cccgaaagcc ggcgcatgct catgcagcgg ctccagatcg
     6601 cctccgggtg gcgcccggac ttctttcgct tctcggacgc ggcgacgctt cccgaggcgc
     6661 agacggccga ggggctgctg cgcgacttga ggaaaggccc ctggccgccc gcgcgcgagg
     6721 aagtggttcc gatcgtgcgc cggctggtgg tgctcggcgc ttacgaccgc gccgccaacc
     6781 tgtcgcgcga catgctcggc gaaccccgca ccaacgcctc caacccggtc ggggacggcg
     6841 atttcaagcg cttcgacgtc tatgccgagc ggccgctcta ccggacgccg ttcgattggg
     6901 agttcggagt ccatccgggc gccaccgcgg tggtcgaacg gccggattcg cccgaggacg
     6961 gcgcgcttta cgtcgagtcc aacggcgcca agaccgtgat gctcgcccgc aaggtggttc
     7021 ggatagcgcc gggcgcgcgt cagctcgctt tcacgatcag gagcagcgac gccggcgcga
     7081 tcgagcgctt ccgctggtcg gtgacctgcc tcgaaaccgg gcagcagctg atccagggtg
     7141 caatgctcgc gccggcgacg aacgttgcca gccagcgggg cctcgccttc accgtgcccg
     7201 cggactgccg gttccagcag ctccagctca gcagcgccga ggcgccgcag cggcagaccc
     7261 tgagcgccta tctcgacaat gtcgcgatca gctgacgagg gcggggcagg gctgaatgac
     7321 tgagatcggc atcgcgccca aggtcgcgcg catctatcct gagatcgcgg caggcggctt
     7381 ttcgcatgtc gacggcacga tcggcttcta ccagcgaatc aacgcgctca tcgggccgga
     7441 catggaagtg ctcgatttcg gcgccggccg cggcgagcag ctggtcgagg atccggtccc
     7501 ctaccgcgcc gccttgtgcc ggctccaggg caaggtcagg cgcttggtcg gcgtcgacat
     7561 tgatccggcc gtgctcagga acccgttcct cgacgaggcg cacgtcatcg atccgagccg
     7621 gccgctcccg ttcgccgacg gccagttcga cctcatctat gccgactggg tgctcgagca
     7681 tgtcgccacc cccgatggct tcgccgccga ggttcgccgc ctgctgaagc cgggcggctg
     7741 gttctgcgcc cgcaccccca atcgctgggg cgtcacgggg ctcggcgcca acctcattcc
     7801 caaccggctc cacaccaagc tcctgaagac gctgcagccg gggcggcagg agcaggacgt
     7861 tttcccgacc acctacaaga tgaacacccg gagcgcggtc cggcgctact tccccgagcg
     7921 cgagtgggag cacagcacct atatctccaa ttccgagccg ccctacgtgc agcgctcgct
     7981 gctgctgatg cgcctggtcc agctgttctg gcgcgcgagc ccccccggct tccacaccac
     8041 cctcaacgtc ttcctccgca agcgcgcata atcgcggctc gcccgaaagc ggcaagcccc
     8101 gtcattcccg cacagcaccc gtcattcccg cgaaagcggg tatcgcactt actgctttac
     8161 cgtcctcgac ctaaagcccg cagggctccg ccaggcgccg cgcctgcgcc gcgcggaagc
     8221 gctcggcgat cagccgggcc atgatgtcat agccctccgc gttgaggtgg acccggtcag
     8281 cccgcagcga gcgcggcacc cagccattgg cgcggtcctg ctcgtcgccc ggcccgcgcg
     8341 ccggaacgcg cgtcaccagg gcttccttgg tggggatgaa atgcttcgga tatttggcgc
     8401 ggatcgcctg caccgccgct tcctgctgtt cgtagcgggg actgccgatc ggcgcgttcg
     8461 gcaccgcccg gtgcggcggc tcgacataga accaggtggg gcattcggac gtgagtgttg
     8521 cggcgatccc ttcgaccgag acgagcggat cgcggtcggt gaggccgccg tcgacgatca
     8581 ccagcgaagc gctccacaat tcgcgaccgc cgccctcggc cccgcccatg tcacggccgc
     8641 cgccccgcag gcgttccagc tgctcggcga ggctgctgcc gccgacggcg tcgaagctga
     8701 tgtcatagcc gggcagcgcc tcgcgcagcg cctgctggaa caagcgcgag gcggcgtagc
     8761 tgtcgccgag cacgtgcacc cgattgggga tcggctcccc gcgctgcgtc gtcatcgccg
     8821 cgatcagcgc gccggccgcg aggcatggga cggcggcggc ggtcagcagc agccacagcc
     8881 acattgggcg gaagcgcggc gaagggttac tctgtacgtc gttcatcggc atcccgtggc
     8941 gtttcccagt tcagatcgaa agcgtcagcc gcctttttga tcccttcgcg cttcgatcgc
     9001 gggcgcgggc ccgaccttct cctcggcggc ttcctgcttt cgcgagcatc ttaagggcat
     9061 tgcggatgaa cgggcgctgg cggcctcggc ggtggccgga cagggccatt tcgcagcaaa
     9121 ggctggacgt accttccctc atgtagcaag gagttgtggc ggcgacgcga ctaagggcga
     9181 gattgcagat gcttaggcaa aatgcggccg tcgggtcgcg tgggcgcggg ctgggggctc
     9241 cgccgcctgc cgccctgtcg ctgcacctcg cggtccattg ctggtcgccg agtgggtttg
     9301 gaatttgagg aacgggccgc ggccgctgcg gctgtcgggg tgagggagtg gtgaagtgag
     9361 accgagcgca accctgcttt cgcaggctgc cccctatatt tcggtggtcg ggatcggagg
     9421 ctcgatcctg gtggctgcca tcgctctgcc gatcggtatt ctcctcgaca gctccggaat
     9481 ggtttacctc agcactgctt ttctggcgtt cgcgattccg gccgcggtgg gagtggctcg
     9541 cggacggaat ttcgatccgt tcgagcccat caacctggtt gccctggcaa tttttttcgg
     9601 gacgagcctc agggccgttt acctgctgac cagcgacaca gagcgggccg cgttcgtgat
     9661 gatgggcacg gacttcgaaa atgtggcggc gaacgctccg tggatgctgc tttcggtggc
     9721 agctctcgtt ctgggctacc tgatcacgcc gtggcgcatc ccgattgaaa agtttcgagc
     9781 ggtgcgatgt tatgccatca accgaacccg gctcaacgta gcgatcggcc tctgcgtgat
     9841 tataacgctc gtggctatcg ccatgctcgc ggcgcgcgag ggcctcgact tctctcaagg
     9901 catattgtcg ctcagcagga agcgggcggc cgtctatacg agcgacgagg gggcggtggt
     9961 ctatggcgtc ggctatgagg ccttcctggg ccagttcgca caatatgcct ttgtcctcct
    10021 ctcctgcgtg gtgctgtcgg ggatcgtcaa acagcgcctc ggcacgctgg tcgcgctggt
    10081 tctgctgggc gtgctgggat gcgtcactcc gttcctcgtg agctcacgaa gcacgatcat
    10141 catcatgctg ctcagcgcgg ccatcgccgc cacttattat ggcaagctca aggcgaaggt
    10201 gctggtggtg atcttcctca cctcgatccc gttggtggtc ggcatgggct atctgaggca
    10261 ggtgaaccat gctcgcgggg ccgtcgagag ggcggagggg acgtcggtga tggacagcat
    10321 catcggctcg ggcaactcgc tcgacttcgt ccggacctcg gcgatcatgg accgcatccc
    10381 ctcggtgcgg ggctaccaat ggggctggac ttacgccgcc ttgttctcgg cgccgatccc
    10441 tcgcgcgatc tggccggaaa agcccaatcc gagccttggt ccatgggtca agcgcgagct
    10501 ttatggcaag ccggttcgca accagggctg gccttcaggc atgatcgccg agggctacat
    10561 aaatttcggg acagtggggc tgattattcc gctattcctg ttcggggcgc tgctgcggac
    10621 cctctatgaa acctgccggc cgcttctcgg ggtctcgctt ccagtcacgg cgatctacgc
    10681 ggtgtcgatc tggcggctgg ggttcggaac gatcggcctc aatttcgccc acggaatgat
    10741 gcagatgctg accgaccttg ccccgatgtt cctgttggtg gtgatagcaa gggcgccgct
    10801 gagaaaggcg attgcggacc gtgtggttgg agcccgccgg ctgccggtct gagagatttc
    10861 aaggggcgga cgacgggtgg ctttgaaatt ggcggccgtt cggctagcca gtttgccaga
    10921 ggggcacgat gctaagcaat attcaggcac ttagggcggt agctgcctat ctggtcgtga
    10981 tgagccacgc cgccggcctg ctcaaggtcg cgggggtcgg cgttcggccg atcgacatcg
    11041 gcgccgccgg cgtcgacatc ttcttcgtca tc
//
LOCUS       DASVRK010000284        27262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_159631, whole genome shotgun sequence.
ACCESSION   DASVRK010000284 DASVRK010000000
VERSION     DASVRK010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 27262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27262
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159631"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..689
                     /locus_tag="VF589_11635"
     CDS             291..689
                     /locus_tag="VF589_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8258274.1"
                     /translation="MLDEHDSSAIVAVADLASARRFYGETLGLELVEEEFEGVLVYRT
                     GATRLVVYRSDYACPERSRRGGTNRANGVVWGVGDELGAIVAALEARGAAFLHYPGIG
                     RLEGNIHVAGDARLVWLEDPAGNILHLNNM"
     gene            792..1406
                     /locus_tag="VF589_11640"
     CDS             792..1406
                     /locus_tag="VF589_11640"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX8258275.1"
                     /translation="MYMFKDPAPLTRAATIALWVYMVLNALTALGTLYLLPVGLELET
                     PAGYAITLLAIGALLAMIACFILVGCWIYRANANAHSFSDYMSISPGGAVGWYFVPIL
                     NLFKPFQAMKETWLASHYPGNWHSEPAPSIVSWWWGLWIVTNILGGLSLRMSASAPDS
                     ANQFIVIVDVISAALNVPLCLVLIAMMKGIAAAQDATHHSEAFA"
     gene            complement(1484..2296)
                     /locus_tag="VF589_11645"
     CDS             complement(1484..2296)
                     /locus_tag="VF589_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX8258276.1"
                     /translation="MIVHLLLAAAAQAAAPQPLDQGQWITADDYPPAARRHDQAGTVG
                     YVVKVDSSGSVTECTISRSSGVAALDLGTCALLTERARFAPASDASGRAAAGEFSSQI
                     KWALPAAGRVPLTPWSQASRLVIARDGEVRSCHTTSGGEVPAEAGDLCRVIGGFPREV
                     RLSMRHGAETDLAVVVAETSMTFDSAELPAARQEPPRRRLISLISSRFDIDEAGEAEN
                     CRIVAREGLSFLQPDLCAKMFDGSFIPDRDANGAPRRRKATVVISIALEAGA"
     gene            complement(2426..2809)
                     /locus_tag="VF589_11650"
     CDS             complement(2426..2809)
                     /locus_tag="VF589_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258277.1"
                     /translation="MANKQVLVIGLDPDFIDFSAPPLAGSGVTAEKIRGGLEGDGAKL
                     NGLGYQADILWVDDGATAERVLRERLEATRYDAVLIGAGIRTLTPYTLLFETLINAVH
                     EAAPQARLAFNTNPADTAEAVRRWV"
     gene            2960..3277
                     /locus_tag="VF589_11655"
     CDS             2960..3277
                     /locus_tag="VF589_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258278.1"
                     /translation="MRAILAVAALAALAGCGEGGVVDDTVRAGLRQSTVEACLAWAPE
                     STMVEAAGISRERLCACAADRILDGGITQLPDLSPDNPEIREAVRQCVGEIGAGAAPG
                     VEG"
     gene            4118..4324
                     /locus_tag="VF589_11660"
     CDS             4118..4324
                     /locus_tag="VF589_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8258279.1"
                     /translation="MPIGTVKFFNIDKGYGFIQPEGGGNDAFVHITAVEAAGMRTLDR
                     DQRVSYELEQDRRGKTSAVNLQAA"
     gene            complement(4482..4991)
                     /locus_tag="VF589_11665"
     CDS             complement(4482..4991)
                     /locus_tag="VF589_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258280.1"
                     /translation="MTNEGSADPTGGINEAALAGRDKEDLGGAGGSSGGGTIAGKGEV
                     SGGTTGGTGGTQGGVPADMPGAETPLGAGAGALAGLDGGGIAAEAAALGGADGSLADA
                     AAGRTAGAERLSGGPTGAEAIGQGARRGDVGSGTAGDKAELGGGGPLGQHGGGGPGGS
                     GSPGGDGRA"
     gene            complement(5004..5459)
                     /locus_tag="VF589_11670"
     CDS             complement(5004..5459)
                     /locus_tag="VF589_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258281.1"
                     /translation="MDPVGLGSVGGSPTGESGGGAPSGPAANGSLGSGTYNERGNVTG
                     PDSPAGGAADLDAQAGQGQGDDLANRLGGGEGRGTGMTGAGTATGDTDADRSERTEAS
                     AQNAAGAGGPDAGSPGGMGGVGASGGTGTGRPPGGVSPVQADVGRQTDS"
     gene            5812..6042
                     /locus_tag="VF589_11675"
     CDS             5812..6042
                     /locus_tag="VF589_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258282.1"
                     /translation="MSQQHEFFLARAAEAKRDAEAATLDNVRDRCLRSEAAWSGMADR
                     AARTEKMRAKAEAAKAAAATEAHVQTMSVSQA"
     gene            complement(6096..6476)
                     /locus_tag="VF589_11680"
     CDS             complement(6096..6476)
                     /locus_tag="VF589_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258283.1"
                     /translation="MAKQSVKNKLPKQIAGVKVPKELRKAGASAVRLMEHPVVSDLVI
                     AALLAAAAALKDNKNVRDAAGAVGDEAGEAAAGTARMAGKAKVALKAAAGAIGQGILE
                     EFSGGGKSSAKAGKSRGKGGRSKA"
     gene            complement(6488..6874)
                     /locus_tag="VF589_11685"
     CDS             complement(6488..6874)
                     /locus_tag="VF589_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258284.1"
                     /translation="MRNCLAFIALSLAAAPAAAQDWRQAREYEVIVSSYDIAPSQIRM
                     DARRPVRLRLINQSGQSHILRAPGFFGAARVRRRDQDAVADGRVTVGPGETRELLLVP
                     APGRYTLRSGNFLYRLLGMSGEIVVE"
     gene            complement(6909..7364)
                     /locus_tag="VF589_11690"
     CDS             complement(6909..7364)
                     /locus_tag="VF589_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8258285.1"
                     /translation="MAEHRATVEWTSDGEFASNRYSRGHEMRFDGGAVVRGSSSPQVV
                     PLPMSDPAGVDPEEALVASASACHMLWFLSLAQAEGFEILSYRDDAVGTMGRDARGRI
                     AITRITLLPVIAFAGGRPTQPELDRLHHEAHERCFIANSLRTEIVVGTR"
     gene            complement(7373..8770)
                     /locus_tag="VF589_11695"
     CDS             complement(7373..8770)
                     /locus_tag="VF589_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX8258286.1"
                     /translation="MNHAKPYFFCGIGGSGMLPLACIVRAQGAEVAGSDRSLDQGRIA
                     GKFAFLEAQGIRLFPQDGSGLTSADQLLVTSAAVEETVPDVQAARRLGTEQVSRPQLL
                     ARLFNDAPVAIAVGGTSGKSTTTGMIGWILHRLGRAPTVMNGAVMKNFVTADTPFASA
                     LIGGGEMFVSEVDESDGSIALYRPRVAVLTNVTLDHKSLDELRGLFLDFTGKAETAVL
                     NLDDEETRRLAARLPAERVATFSFADAAADLFGHDVVEEPLAISFAVRVSGAEGSIPV
                     RLGVPGRHNAANALAALAAVRACGVDLGEAAAAVAGFVGLRRRFDIVGTRSGVTVIDD
                     FAHNPDKIAATLATLHAFPGRLLMMFQPHGYGPLRTMKRELIEGFAAGLGQEDVLVMP
                     DPVYFGGTVEREVGSGDIVAGVRAAGRQAEHVPERAACGERLRALSRPGDRIIVMGAR
                     DDTLSDFAAELLASL"
     gene            8824..9633
                     /locus_tag="VF589_11700"
     CDS             8824..9633
                     /locus_tag="VF589_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX8258287.1"
                     /translation="MRIGVVAPSTPIDREVADRVLALAAAAFPEVELLFHRQCFAVHN
                     HFAGPDAERAEAFLEVANDAGFDALWFARGGYGSCRIAEDVLARLGPAARAKAYLGYS
                     DAGFLLAGLYAAGFADVAHGPMPGDVKRPDGEGAIARALMWLTARDAAALEPGLAGSR
                     NAAFNITVLSQLLGTPLQPDLGGHVLLIEEVAEYMYRIDRSMFHITSNPTVRRAAGIR
                     LGRCSDIPANDRDFGEGEEQVVRHWCARSGIPYLGRADIGHDAANKVVPFG"
     gene            9722..10015
                     /locus_tag="VF589_11705"
     CDS             9722..10015
                     /locus_tag="VF589_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX8258288.1"
                     /translation="MLDPKSKSSGKKSGGSDGLQRPLKPSADLAKITGSDNIARSDVV
                     SKMWEYIKKNNLQNPENKREIMADDNLRPIFGGQDKVSMFEMNKHLSKHLSQP"
     gene            complement(10114..10839)
                     /locus_tag="VF589_11710"
     CDS             complement(10114..10839)
                     /locus_tag="VF589_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX8258289.1"
                     /translation="MRLLSAFRTLVWLTLIGFGAMLLYGYARNHPEDLPWTEIDLTAP
                     VGAFTGRKLVGLREEGPQCQALLEQAGVRFDPLPPRSSGEHCGYSDAVRLTGGAVTLR
                     YRPEGLGASCPVAAALALWEWHVVQAAALEHLGLKVVAIDHFGSYSCRRLYGRSEGAW
                     SEHATANAVDIAGFRLEDGSRVSVLGDWDDDGAKGAFLSEVRDGACGLFATVLSPDYN
                     EAHRDHLHLDQADRGAMGWRGCR"
     gene            complement(10876..11634)
                     /locus_tag="VF589_11715"
     CDS             complement(10876..11634)
                     /locus_tag="VF589_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8258290.1"
                     /translation="MDDLPDPIPAATLVLMRPSAGGAPELLMIERTRNMAFAAGALVF
                     PGGRIDPDDEWLAERIGQGFDHPAARVAAVRETIEETGVAPALLPAPDPATISQIRQE
                     LEEGTPFSALLEGFGLGLDLAALTPFARWCPTFKEARRFDTLFFIAEAPAAAPAAPVA
                     EAEAVRMLWASADEVLAEADAGRARIIFPTRRNLERLARFASIEEARADAARHPLAKI
                     VPWIEERGGERWLCIPEAAGYPVTAERLETARRS"
     gene            11836..12693
                     /locus_tag="VF589_11720"
     CDS             11836..12693
                     /locus_tag="VF589_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8258291.1"
                     /translation="MAVAVAIALLVLASIAFHFLSPWWMTPLASNWGSIDDALIITLY
                     ICAIAFVGLNLFMAYALLKYRHRPGHSAHYEPENARLERRLTFWTGIGIAGMLAPGLI
                     AWNEYVTVPKEATVIEATGQQWQWAFRFPGQDNVLGTAAVAHMTADNPFGVNPRDPHG
                     RDDVLIETNELHMPIGKPVKMVLRSKDVLHDFYVPQFRAKMDLVPGIVTYFWMTPTKL
                     GTYEILCAELCGVGHHEMRGTVVVQDQQAFDAWMGEQMTFGQLLAQNQGDKATQLASA
                     DVKPTIGGR"
     gene            12694..14451
                     /locus_tag="VF589_11725"
     CDS             12694..14451
                     /locus_tag="VF589_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8258292.1"
                     /translation="MATITHPTLADDLTPLHHPKTWIGKYVWSQDHKVIAIQYTLTAV
                     SIGLVALFLSMLMRLQLAYPGSFEFIQPGNYLQSVTMHGMIMVVYLLTALLLGGFGNY
                     LIPLMVGARDMVFPFVNMLSYWVYLLSVIVLAASFFVPGGPTGAGWTLYPPQTILEGT
                     PGHEWGIVLMLASLAIFIVAFTMGGLNYVTTVLQARTKGMTLMRMPCSVWGIFTASIM
                     GLLAFPALFVAAIMMLLDRLAGTSFFMPAILSMGKDAGYEGGSPILFQHLFWFFGHPE
                     VYIVALPAFGIVSDLISVHARKNLFGYRMMVWAIVFIGALSFFVWAHHMYVSGMNPYF
                     GFFFATSTLIIAIPTAIKVYNWLLTLWRGDIHLRVPMLFAIGFLFTFIHGGLTGLFLG
                     NVSVDVPLSDTFFVIGHFHMVMGVSPILVIFGAIYHWYPKITGRMWNDAVAKWHFWIT
                     FVGVYAIFLPMHYLGFLGVPRRYYALGETAFIPPSAMVANQMITIAALIVGVAQLLFF
                     WNMAWSLFRGEKAPPNPWGAASLEWQTPDTPPAHGNWGPSLPVVHRWAYDYSVPGYPR
                     DFIPQNEPHDPAMKRHHET"
     gene            14448..15080
                     /locus_tag="VF589_11730"
     CDS             14448..15080
                     /locus_tag="VF589_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX8258293.1"
                     /translation="MSIFSRLAEKSWLTPGADYDDPTGYRPSAGRVAMVVYFVVATVI
                     FGLVSSAYVMRMGMPGMGHGPVGDWRPMPEPPLLWVNTGILLLASLAWEAARSALRRG
                     RVDGARGALIGAGLLGLAFLAGQLLLWRQYGEAGYYLQSNPANSFFYLITGLHGAHLI
                     AGLFFWLRAYARAGDRTDAFAAGQAVDVSAAYWHFLLLVWLVMLGLLVST"
     gene            15098..15790
                     /locus_tag="VF589_11735"
     CDS             15098..15790
                     /locus_tag="VF589_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III family protein"
                     /protein_id="HEX8258294.1"
                     /translation="MSTVIHQGENAGGPPQQLREFASDWSTDQEVFKQVHWGKAMMWI
                     FLLSDTFIFSCFLTSYMTMRASVTVPWPNPSEVFALTIAGESIPLILIAIMTFVLISS
                     SGTMAMAVNFGYRGDRRTTAFLMFATALFGLTFVGMQAFEWTKLIHEGVRPWSNPFGA
                     EQFGASFFMITGFHGLHVSAGVIMLFVVAFKVLRGQYERSGDYSAVEIAGLYWHFVDL
                     VWVFIFAFFYLW"
     gene            15801..16220
                     /locus_tag="VF589_11740"
     CDS             15801..16220
                     /locus_tag="VF589_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX8258295.1"
                     /translation="MNHGTDTAHAPAAHGAGHGVAHGAGHAAPRAAPAHDPGQQHPLG
                     VYFKVWGLLFVLSALSYAVDYFRAEGIMRWVLILIFMFLKAGLIIAIFMHLAWERMAL
                     IYAILVPPLALITLVWLMLIESDYTFWTRILFFDGGG"
     gene            16220..16939
                     /locus_tag="VF589_11745"
     CDS             16220..16939
                     /locus_tag="VF589_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX8258296.1"
                     /translation="MDGWEWTKLGGALGGALAVALGGWWFAGLVMYPAYPEGRGYQVE
                     GVAPVDLAAMQRSWPAGMQQPDDPTELRGYMANIEKAVLPAPATGAGPLAPAAPVDLG
                     TLLAAADPARGKGTARTCGSCHTFEQGEADRVGPNLWAVVGRPIGGKSGYGYSAAIAA
                     HGGAWTYQELDAYLASPGKAIPGNKMAFNGIRNPKDRANLLAYLGTLNATPSPFPAAK
                     PAGTPEGAASTTGAGAVPTSR"
     gene            complement(16943..17914)
                     /gene="glk"
                     /locus_tag="VF589_11750"
     CDS             complement(16943..17914)
                     /gene="glk"
                     /locus_tag="VF589_11750"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX8258297.1"
                     /translation="MTEIVAADIGGTHARFALAEVVDGRVAALGEPCTLATADHAGLE
                     DAWAEFARRTGRPLPAAAAIAVAAPIEGDTIKLTNNPWTIDRTGLPTRLGVSRLRLVN
                     DFGAVAHAVAQLDPVHFRHLCGPDRPLPEEGLISIVGPGTGLGVAQLLRRGGRAEVIE
                     TEGGHIGFAPADAFEDALAAGLRPRFGRVSVERIVSGAGLAVIYSALASAGGRTPAYA
                     EDKELWAAALAGEDALAGEALERFCGALGAAAGDIALAQGAAAVAIGGGLGLRLADRL
                     PNSAFHERFVDKGRYRQRMERMPVRIVTHEQPGLYGAAAAFADRYAD"
     gene            complement(17961..18512)
                     /locus_tag="VF589_11755"
     CDS             complement(17961..18512)
                     /locus_tag="VF589_11755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002014.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEX8258298.1"
                     /translation="MKAPRDAIRLSKFLSYVLRHSPEAAGISLDREGWAEVAQVLEAA
                     RRKGFEVDEARLGAVVANSDKKRFTLSEDGARIRAAQGHSTPQVEIAFAPMAPPALLY
                     HGTAETNLPSILSSGLEPRKRHYVHLSPDAATAVQVGRRHGKPMVLRVDSGAMHAAGH
                     IFYRADNGVWLTRSVPPEFISEP"
     gene            complement(18957..19032)
                     /locus_tag="VF589_11760"
     tRNA            complement(18957..19032)
                     /locus_tag="VF589_11760"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18997..18999),aa:Ala,seq:cgc)
     gene            19288..20583
                     /locus_tag="VF589_11765"
     CDS             19288..20583
                     /locus_tag="VF589_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX8258299.1"
                     /translation="MGYGDDRYGGGNRSGGDDRWRDRGQGGNWRGERGGFGGGRPEGD
                     QDRGFFERAGEQVRSWFSDDDDDRGRGGGNYSRGGGGGGSGSFGGGWGNQQGESWNRD
                     RPGMSSGGGYGAGNRDYGNDWGSGSGGGGGSGSGSGGYARDYQSSGGGSDRFEEGSFG
                     QEARSSEVWGGSGFGGGTDHGRRFDRVDPGAVGTHGAHPMSSPVGGAYGAGYGASMGG
                     GYGSSARTAAMLGGGGRGATSGYGSSGASYAGPQGGRQGGGQQGGGQQGGFGGGGRPG
                     IHDPHYSEWRSRQIEQLDRDYDEYRREHQSKFEQEFGGWRQKRQGQRSSLGRITEHME
                     VVGSDGQRIGVVDKVGGDRIILTKNDENAGGRHHSIPCSWIENVDERVTVNKTAQDAM
                     NAWRDEEQNRALFEREDQGSEGPHMLNRSFSGTYSDRDD"
     gene            20727..21719
                     /locus_tag="VF589_11770"
     CDS             20727..21719
                     /locus_tag="VF589_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8258300.1"
                     /translation="MTGTRAWHLASRPSGMPDMTNFELREMELPPLEDGMVRVENIWL
                     SVDPYMRGRMNDVKSYVPPFEVGAPLQGGAVGKVVETRSPHFNVGDTVFHMLGWREQA
                     VAPAAQFNKVPAMGVPDPQWLGNLGLTGATAYFGLLRVAEAREGEIVFVSAAAGAVGS
                     AVVQIAKARGMTVIGSAGGEEKCAWVRELGADAAIDYKAGPVVKALTAAAPKGIDVYF
                     DNVGGEHLDAAFAAARNRARFAICGMIDNYNAGPPPSFRYIMRVIAARIQLRGFIYTD
                     YLPEMGDFYRDMGGWIASGQVQSRDTVREGIEATPQAFLDLFTGGNMGKMLVRL"
     gene            complement(21731..21904)
                     /locus_tag="VF589_11775"
     CDS             complement(21731..21904)
                     /locus_tag="VF589_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258301.1"
                     /translation="MVRTGWRRWAAAILALVSGAMAAAQPAERPEKVVAYVGASVLDV
                     RSGEMRRGLTIVK"
     gene            22001..22489
                     /locus_tag="VF589_11780"
     CDS             22001..22489
                     /locus_tag="VF589_11780"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX8258302.1"
                     /translation="MARPITFIVARADNGVIGKDGTLPWHLPADLKRFKRLTMGSAMA
                     MGRRTFDSLPGLLPGRRHIVLTRTPGWSAPGAEPVHSVKEAIARAGAHPLAVIGGAEI
                     FALFEPAADAVELTEVHTDAEGDTLMAPFDPARWREVFREPHPAEEGRPAYSFVRLER
                     NG"
     gene            22486..23655
                     /locus_tag="VF589_11785"
     CDS             22486..23655
                     /locus_tag="VF589_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX8258303.1"
                     /translation="MMRRVALGLLLLLVVAVAGLLLIGPGMVEKSLNTIVEQELPAPS
                     PAVQALHRRLAIADLHADTLLWQRSLLDPATRGHVDLPRLEAGNVALQIFSSVTKSPK
                     GLNYDRNAADSDDITALALVQLQPPRTWSSLAERSLWHSAKLHRAEAASGGRLRIVRS
                     AADVRRLIADRAAGKPVTGAMLSVEGLHNLEGKLANLARLDAAGFRMAGLVHFFDNEV
                     GGSMHGLAKGGLTPFGRQVVRAMEARGMIVDLAHASPATVRDVLAMARRPIVSSHGGV
                     QATCKVNRNLTDEQVRGIAATGGLVGIGYWDGAVCSADPAAIAAAIAHVRGLVGIGHV
                     ALGSDFDGSVTTGFDTSQLVQVTRALAARGFTEAEIAAVMGGNLLRLLERALPQR"
     gene            23808..24734
                     /locus_tag="VF589_11790"
     CDS             23808..24734
                     /locus_tag="VF589_11790"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX8258304.1"
                     /translation="MERLEGGSAVPDHLRGGIVALGNFDGFHAGHQAVVGRAVERARA
                     ERRPVLVATFDPHPVRFFKPDLPPFRLTTLDQRERLFGAAGADAMIVFRFDAELAATS
                     AEDFVAGRLAGRIGAAGLVTGEDFTFGKGRSGNIEVLRSLGAAHGLSVDAVAPVAADG
                     EPVSSSRIRDALQAGDCETAARLLTRPFAIEGVVEHGDKRGRELGYPTANLALGKYLR
                     PRYGVYAVRGRLADGRVLAGAANLGIRPTFQPPRELLEPYFFDFSGDLYGQPLEVELI
                     AFLRPERRFDRLDTLTAQMDADCVEARRRLTA"
     gene            24731..25690
                     /locus_tag="VF589_11795"
     CDS             24731..25690
                     /locus_tag="VF589_11795"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX8258305.1"
                     /translation="MKIAKWALILIALAAVILSLTNASWIAPKPTGKLILVAHRGTGQ
                     PAAAGASGDCPGVAITASGHNYLENSVHGMKAAAAFGGDALALPVQRTSDGLIVIFSD
                     PALDCRTDVSGPVAARSLAELKRLDAGHDYTPDGGRTFPVRGRIGGIQTLDEVLSEMR
                     GQRFIFRFVGDDPAAADALAATFERAGAKIDPGTGFLGPKAATDRMKQLAPEAWVYDP
                     AQSRACLAGYARTGWAGIIPPECRDAVLVVPYDSGWTIWGWPYRFLDRMAGVGAEVML
                     VASLDANGHPLGLTEPQQLGDVPRHFRGMLWVENMYEVGRALN"
     gene            25737..>27262
                     /locus_tag="VF589_11800"
     CDS             25737..>27262
                     /locus_tag="VF589_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8258306.1"
                     /translation="MSEKPDYKDTVFLPKTDFPMKAGLAQKEPAILARWAETGLYEQI
                     RAARAGAERFILHDGPPYANGDIHIGHAMMKTIKDFILRSQSLMGKDAPFIPGWDCHG
                     LPIEWKIEEQYRKQKKDKDEVPPREFRAECRAYAERWVEVQSAQFQRLGILGDWADPY
                     RTMDYASEAVIAGELLKFAMASKLYRGAKPVMWSPVEKTALAEAEVEYEDIDSTQIDV
                     AFEIVESPIPELVGAHAVIWTTTPWTIPVNQAIAYGPDIEYVLWANPVGGAEWIGSGS
                     EMPLASVRYLLIAEPLLLAFLDRTNGGNARPPSAGLEWYGDPVPPFTWRGKGFDLAGT
                     VARHPMHHLGGFFARPRPFLPGDFVTTDAGTGLVHMAPDHGEDDFLLCKAHGIEPVFA
                     VEADGKYRADWEWLGGQGSVINPKFNAPDGPINSDLREAGALVAASADFRHSYPHSWR
                     SKAKLIFRATPQWFIPMDQSPSPPSGGEGQPARSGGRERGESGGLSSGGAPPPPPPPP
                     P"
BASE COUNT         4125 a         9945 c         8882 g         4310 t
ORIGIN      
        1 tttgccgacc ggttcgacgg ggccggagcc tgtcccgcgc gacgacggcg caggcctcga
       61 aaccccctcc acagtgacag ctatgacaaa ttcacctcag ccgaatgcct cggacggcgc
      121 cgcgcaatcc gttccggccg ccgccgccgg cgccgccccc gcccacgcac ccccgcaaac
      181 gccctccaca gcggcaggtt tgacaagttc acccgccggg cgcgaagaac ccgccacggc
      241 cgaaccgccc caacccggcg cgccggccgc cccggccagg gagaaacgcc atgctcgacg
      301 aacacgacag ctccgccatc gtcgccgtcg ccgatctcgc tagcgcccgg cgcttttacg
      361 gcgaaacgct tgggctcgaa ctggtcgagg aggagttcga gggcgtgctc gtctaccgca
      421 ccggcgccac ccgcctcgtc gtctaccgct ccgactatgc ctgtcctgag cgaagtcgaa
      481 ggggcggcac caaccgcgcc aatggcgtcg tctggggcgt cggcgacgag ctcggcgcca
      541 tcgtcgccgc gctcgaggcg aggggcgccg ccttcctcca ttatcccggc atcggccggc
      601 tcgagggcaa catccacgtc gccggcgacg cgcggctggt ctggctcgag gatccggccg
      661 gcaacatcct ccacctcaac aatatgtgag cctcccgccc cggccgccgc cccctcattt
      721 tcccactggc gtccgcaaag gcttgatgta ggcttcgcgc cgggcgccgt tcggcgatgg
      781 gggaaatcag gatgtacatg ttcaaggatc cggcgccgct cacgcgcgcc gcgaccatcg
      841 ctttgtgggt ctatatggtg ctgaacgcgc tcaccgcgct gggcacttta tacttgctgc
      901 cggtcggcct cgaattggaa acgcccgccg gctacgcgat cacgctgctg gcgatcggcg
      961 cgctgctggc gatgatcgcc tgcttcatcc tggtcggctg ctggatctat cgcgccaatg
     1021 ccaacgcgca cagcttcagc gactatatgt cgatcagccc cggcggcgcg gtcggctggt
     1081 acttcgtgcc tatcctcaat ctgttcaagc cgttccaggc aatgaaggag acttggctcg
     1141 caagccacta tccgggcaac tggcattcgg agccggcgcc ctcgatcgtg agctggtggt
     1201 ggggcctgtg gatcgtcacc aacatcctcg gcggactgtc cctgcggatg agcgccagcg
     1261 caccggactc ggccaaccag ttcatcgtca tcgtcgacgt catctcggcc gcgctcaacg
     1321 ttccgctctg cctcgtcctc atcgccatga tgaagggcat tgccgccgcc caggacgcga
     1381 cccatcacag cgaggcgttc gcctgacgcg ggagcggcgg cagcgggaaa gcggatctac
     1441 cgctattggc gcgccgcggg gggcccggcg ccgatccgcc gcttcaggcc ccggcctcca
     1501 gcgcgatcga tatcacgacc gtcgccttcc tgcgcctcgg tgccccgttc gcgtcgcggt
     1561 cggggatgaa cgagccatcg aacatcttcg cacagaggtc cggttggagg aagctcaatc
     1621 cctccctcgc gacgatcctg caattctccg cctcgcccgc ttcgtcgatg tcgaagcggc
     1681 tggagatgag cgagatcagg cgccggcgcg gcggttcctg ccgggcggcg ggtagctcgg
     1741 cgctgtcgaa cgtcatgctg gtttcggcga ccaccaccgc caagtcggtc tcggcgccgt
     1801 gccgcatcga cagcctcacc tcgcgcggga agccgccgat cacccggcac aggtccccgg
     1861 cctccgctgg cacttcgccg ccgctggtgg tgtggcagga tcggacctcg ccgtcgcgcg
     1921 cgatgacgag gcggctcgcc tgcgaccagg gggtgagggg gacccgtccg gcggccggca
     1981 aagcccactt gatctggctc gaaaactcgc ccgccgcggc tcggccggat gcgtcggagg
     2041 caggggcgaa ccgcgcccgc tccgtgagca gcgcgcacgt gccaaggtcg agcgcggcca
     2101 cgccggagga ccggctgata gtgcattcgg tgacgctgcc ggaactgtcg accttcacga
     2161 catagccgac ggtgccggct tgatcgtgcc gccgcgccgc gggcggataa tcgtccgccg
     2221 tgatccattg gccctggtcc agcggctgcg gcgccgcagc ctgcgccgcg gccgcaagca
     2281 aaagatgcac tatcaccgaa ttccccccgc ctgcgaacgg gcccgtgtgc cgcctatggc
     2341 gccccgccgc aagcgccgcc gccccggccc ggctcgagcc tgggtcgact aacgacgaac
     2401 atagccgaac atcaggctag cctcgtcaca cccatcgccg caccgcctcg gccgtgtccg
     2461 ccgggttagt gttgaatgcg agcctggcct gcggcgccgc ctcgtgcacc gcgttgatca
     2521 gcgtctcgaa cagcagggta tagggcgtca gcgtgcggat gcccgcgccg atcaagaccg
     2581 catcgtagcg cgtcgcctcg agccgctcgc gcagcacccg ctccgcggtc gcgccgtcgt
     2641 cgacccacaa tatgtccgcc tggtagccga ggccgttgag cttggcgccg tcgccctcga
     2701 gccctccccg gatcttttcg gcggtcacgc cggaccccgc cagcggcggc gccgaaaagt
     2761 cgatgaagtc gggatcgagc ccgatcacca gcacctgctt gttcgccatc cccacatctc
     2821 cctttggtcc ttttgcccct ctgcaagtgg ggcaccgccg cgcggcttca agcgagcgcg
     2881 atccacgggg gaggggagcg ctctttacat ccgcccccgc ggaggcgtta gaagcggctt
     2941 gagcttccgg aggaaagata tgcgcgccat ccttgccgtc gccgccttgg ccgcgctcgc
     3001 cggctgcggc gagggcggag tcgtcgacga cacggtccgc gccggcctca ggcagagcac
     3061 ggtcgaggcc tgcctcgcct gggcgcccga atcgacaatg gtcgaggcgg cggggatcag
     3121 ccgcgagcgg ctgtgcgcct gcgccgccga ccgcatcctt gacggcggca tcacccagct
     3181 tcccgatctc agccccgaca atcccgagat ccgcgaagcc gtccgccaat gcgtcggcga
     3241 gatcggcgcg ggcgccgccc ccggcgtgga gggctgagcc cggccttgcg gccgccggga
     3301 ggcgcagcgc gattcgcgcg gtcgggcgcc cgttttggac tgatccaagt tatccccgtt
     3361 atactagtta tccacaggac gaatccgctt gcgcgactcg gtaggagagc gcagcttctg
     3421 gatgtggccg ggagcggccc cgccggaagg acgcaagtcc ggaaggacgg aaaagctcca
     3481 gggcattgcc accgcggcag gcggcggaca tccgcaagga tgaaagccgg cgaagcgaat
     3541 ggcagggcga ccgcaggaag aagcgcgagg cacaggcgca agccgatgcc gaacccactt
     3601 tccgaatgga agtccggcgg caccggagcc ttcgggcata aggaccgcga aagggaattc
     3661 cgacgaggcc cgcctcgaag gatgaaccgc cggggacccg cttgggccgc aaggcttcga
     3721 acgggtgacc cgagagggac cggaaacggg ccgcaaggcc tcgctccaac cttctccgcc
     3781 cggaaaagga accgtgcctt cgggcatggc aaccgatcag ggaaagtggg ggctggccgc
     3841 aacgccagcc cccattactt tgtgcggcgc cgcccgcccg ccgcgcttcc ccgcccgcca
     3901 ttttcgactc cgccaatatg tccgcggccg cgcccgccat ggccgccaag attcggtgcg
     3961 ggcttgctaa agtcccggaa atagtccacc accgccgggc tgaagtcgcc tgcgacggat
     4021 cttggcgctg cacgctcctt ttccttctct acacgctact catgcatggc aggtcgccag
     4081 gcctcgccat atcgacacta agctcaagga aaagatcatg ccgatcggca ccgtaaaatt
     4141 cttcaacatc gacaagggct atggcttcat ccagcccgag ggcggcggca acgacgcctt
     4201 cgtccacatc accgccgtcg aggcggccgg gatgcgcacg ctcgatcgcg accagcgcgt
     4261 cagctacgag ctcgagcagg accgtcgcgg caagaccagc gccgtcaacc tccaggctgc
     4321 ctaatcctat ccctcccccg ctccggcggg ggaggcgatg cggcgtcggc cctcgcgccc
     4381 gcgaccacta atctcccggc gcgcttgacc aggccggcgg cccacgatcg cgaccccagc
     4441 ggcacgctga accaggtccg actcctccca atccctgaaa ttcaggcgcg accgtcgccg
     4501 cccgggctgc cgctgccgcc aggcccgccg ccgccatgct ggccgagcgg accgccgccg
     4561 ccgagctcgg ctttgtcgcc cgccgtcccg gatccgacgt cgccgcgccg cgcgccctgg
     4621 ccaatcgcct cggccccggt cggtccgccg ctcagccgct cggccccggc cgttcgtccg
     4681 gccgccgcat cggcgagact tccgtccgcc ccgcccagag ccgccgcctc cgccgcgatg
     4741 ccgccgccgt cgaggccggc cagcgcgccc gcgccggcgc cgagcggagt ctcggccccc
     4801 ggcatgtcgg cgggaacgcc gccttgggtt ccgccggtcc cgccggtggt tccgccggag
     4861 acctcgcctt tgccggcgat cgtgccgccg ccactggagc cgcccgcgcc gcccagatct
     4921 tccttgtcgc gtcccgccag cgcggcctcg ttgatgccgc cggtcgggtc cgccgagcct
     4981 tcattcgtca tcgcctgatt cctttagctg tcggtctggc gccccacgtc ggcctgcacc
     5041 gggctgacgc cgcccggcgg gcggccggtg ccggtgccgc cgctcgcgcc cactccgccc
     5101 atcccgcccg ggctgccggc atcggggccg ccggcgccgg ccgcgttctg cgccgaagct
     5161 tcggtccgct cgctgcggtc ggcgtcggta tcgccggtcg cggttccggc gccggtcatg
     5221 ccggtgccgc ggccttcgcc gccgccgagc cggttggcga ggtcgtcgcc ctggccttgt
     5281 ccggcctgcg cgtcgaggtc ggcggcgccg ccggccggac tgtccggccc ggtgacgttg
     5341 ccgcgctcat tatacgtgcc ggagccgagc gatccattcg ccgccggccc gctcggcgcg
     5401 ccgccgccgc tctcgccggt cgggctgccg cccacgctgc cgaggccgac cgggtccatg
     5461 tcgcctcccg ctgcgccgtc gcgcctgccg ccaccacctg ctcgcgaata tcgccgccgc
     5521 tcttgtcctg atcctgctgg tcgctcattt gaagctcctc gttcaacctg ccggcgcgcg
     5581 cacggtctcc gtcgcgccct cagccgggga aacgagccgc gggcccgctg gttgccccgc
     5641 cgcgtcgcga agcgatcatc ccgcggtcgc acccgatcgc cgcagacgcc cgtaaaaccg
     5701 ccatatttgc ggaaatcgcc ggatgagcgc ataggggtta atagcccccc taccgatctt
     5761 ggccgcagtg cggccgccgt cgtcctcacc ctccccaggt aaaggtcccg aatgtcacag
     5821 caacacgaat tcttcctcgc ccgcgccgcc gaagccaagc gcgacgccga ggcggcgacc
     5881 ctcgataacg tccgcgatcg ctgcctgcgc tccgaagcgg cctggtcggg catggccgac
     5941 cgcgccgccc gcaccgaaaa gatgcgcgcc aaggccgagg ccgccaaagc cgccgcggcg
     6001 accgaagcgc acgttcagac gatgagcgtt tcgcaagcct gacgcttacc gctcagcata
     6061 aagccggccg cggcatagcc cgcgccggct cctgctcagg ccttgctgcg gccgcccttg
     6121 ccgcgggact tgccggcttt cgcgctcgac ttaccgccgc ctgagaattc ttcgagaatg
     6181 ccctgaccga tcgcgccggc cgcggccttg agcgcgacct tcgccttgcc cgccatccgc
     6241 gccgttccgg cggcggcctc gccggcttcg tcgccgaccg cgccggccgc gtcgcgcacg
     6301 ttcttattgt ccttgagcgc cgctgccgcc gccagcaggg ccgcgatgac gaggtcgctc
     6361 accaccggat gctccatcag ccggaccgcg ctcgcgcccg ccttgcgcag ctccttgggg
     6421 accttcaccc cggcaatctg cttgggaagc ttatttttga cactctgctt ggccattgct
     6481 gacgctccta ttcgacgacg atctcgcccg acatgccgag caaccggtaa agaaaattgc
     6541 cgctccgcag cgtgtagcgg ccgggcgcgg ggacgagcag cagctcgcgc gtctcgcctg
     6601 gccccaccgt gacccggccg tccgccaccg catcctggtc gcgccgccgg acgcgggcgg
     6661 ccccgaagaa accgggcgcc cgcaggatgt ggctctggcc gctctggttg atgaggcgca
     6721 gccgtaccgg ccggcgggcg tccatgcgga tctgcgaagg tgcgatgtcg tagctcgaca
     6781 cgatcacttc atattcgcgc gcctgccgcc aatcctgggc cgcggccggc gccgcggcga
     6841 ggctgagggc gatgaaggcg aggcaattgc gcatcggcaa cattcctgaa cgagaatcgc
     6901 cggcgacgct agcgggttcc gaccacgatc tcggtccgca gcgagttggc gatgaagcag
     6961 cgctcgtgcg cctcgtggtg gagccggtcg agctccggct gcgtcggccg gcccccggcg
     7021 aaggcgatta cgggcagcag ggtgatgcgg gtgatcgcga tccgcccgcg cgcgtcgcgg
     7081 cccatcgtgc cgaccgcatc gtcgcgatag ctgaggattt cgaacccctc agcctgggcc
     7141 agcgacagga accacagcat gtggcaggcc gaggccgatg cgaccagcgc ttcctccgga
     7201 tcgacgccgg ccgggtccga catcggcagc ggcacgacct gcggcgagga cgagcctcgc
     7261 accaccgcgc cgccgtcgaa gcgcatctca tgcccccggc tgtagcggtt cgaggcgaac
     7321 tcgccgtccg aggtccattc gaccgtcgct ctgtgctccg ccatgtttcc cctcagaggc
     7381 tcgccagcag ttccgccgcg aagtccgaca gggtgtcgtc gcgcgcgccc atcacgatga
     7441 tccggtcgcc cggccggctg agggcgcgga gccgctcgcc gcaggccgcc cgctcgggca
     7501 catgctccgc ctgcctgcct gcggcgcgga cccccgcgac gatatcgccg ctgccgacct
     7561 cgcgctcgac cgtgccgccg aaatagaccg ggtccggcat caccagcacg tcctcctggc
     7621 caagccccgc cgcgaagccc tcgatcagct cgcgcttcat ggtccgcagc ggcccgtagc
     7681 catgcggctg gaacatcatg agcagccgcc cgggaaaggc gtggagcgtg gcgagcgtgg
     7741 cggcgatctt gtcggggttg tgggcgaaat cgtcgatcac cgtgacgccg ctccgcgtgc
     7801 cgacgatgtc gaaccggcgc cgcagcccga cgaagccggc gaccgccgcg gcggcctcgc
     7861 ccaggtcgac gccgcaggcg cgcaccgccg ccagcgcagc gagcgcattg gcggcattgt
     7921 ggcgccccgg aacgccgagt cggaccggga tcgatccctc cgcgccgctc acccgcaccg
     7981 cgaaactgat ggcgagcggc tcttccacca catcgtgccc gaacaggtcg gccgccgcgt
     8041 cggcgaagct gaacgtcgcc acccgctcgg ccggcagcct cgccgccagc cgccgcgtct
     8101 cctcatcgtc gaggttgagc acggcggtct cggccttgcc ggtaaagtcg aggaacagcc
     8161 cgcgcagctc gtcgagcgac ttgtggtcga gcgtgacgtt ggtcagcacg gcgacccgcg
     8221 gccggtagag cgcgatcgac ccgtcgctct cgtcgacctc gctgacgaac atctcgccgc
     8281 cgccgatcag cgcgctggcg aacggcgtgt cggcggtcac gaaattcttc atcaccgccc
     8341 cgttcatcac ggtcggcgcg cggcccaggc ggtgcaggat ccagccgatc atgccggtgg
     8401 tggtggattt gccgctggtg ccgccgaccg cgatcgcgac cggggcgtcg ttgaacagcc
     8461 gcgcgagcag ctgcgggcgg ctgacctgct ccgtcccgag ccggcgcgcc gcctgcacgt
     8521 cgggcaccgt ctcctcgacc gcggccgagg tgacgagcag ctggtcggcg ctggtgaggc
     8581 cgctgccgtc ctgcgggaac aagcggatgc cctgcgcctc gaggaaggcg aacttgccgg
     8641 cgatgcggcc ctggtcgagc gagcggtcgg acccggccac ttcggcgccc tgggcgcgga
     8701 cgatgcacgc cagcgggagc atgccgctgc cgccgatccc gcagaagaaa tagggtttgg
     8761 cgtggttcat cggctcgccc tattggcaag cggcggccaa ttcaaacgcc gagggagccg
     8821 gggatgagaa ttggagtggt cgcgccgagc acgccgatcg accgcgaggt ggcggaccgg
     8881 gtgcttgcgt tggccgccgc cgcctttccc gaggtggagc tgctgttcca ccgccaatgt
     8941 ttcgcggtcc acaaccattt cgccgggccc gatgccgagc gcgccgaagc cttcctcgaa
     9001 gtcgccaacg atgccggctt cgacgccttg tggttcgccc gcggcggcta cggctcctgc
     9061 cggatcgccg aagacgtgct ggcgaggctc ggccccgccg cgcgcgccaa ggcctatctc
     9121 ggctatagcg acgccggctt cctgctcgcc ggcctctacg ccgccggctt cgccgatgtc
     9181 gctcacgggc cgatgcccgg cgacgtcaaa cggccggacg gggagggcgc catcgcgcgc
     9241 gcgctaatgt ggctgacggc gcgcgatgcg gcggcgctcg agccggggct cgccggcagc
     9301 cgcaacgccg ccttcaacat caccgtgctc agccagctgc tcgggacgcc gctccagccc
     9361 gacttgggcg gccacgtcct gctgatcgag gaggtggccg aatatatgta ccggatcgac
     9421 cgctcgatgt tccacatcac cagcaatccc acggtgcgcc gcgccgccgg catccggctc
     9481 ggccgctgca gcgacatacc ggccaacgat cgcgacttcg gcgagggcga ggagcaggtg
     9541 gtgcgccact ggtgcgcccg ctccggcatc ccctatctcg ggcgcgccga catcggccac
     9601 gatgccgcga acaaggtcgt accgttcggc tgaggcggtc gagttgcgcg aaaccgccgc
     9661 cgagaccatt gcattgcccg cccgattgcg ctctatcagg gaaaattcac aggagggccg
     9721 aatgctggat ccaaagtcaa agagcagcgg gaagaagtcg ggcggatcgg acggtctgca
     9781 acggccgttg aagccgtcgg ccgatcttgc caagatcacc ggatcggaca acatcgcgcg
     9841 cagcgacgtc gtcagcaaga tgtgggaata tatcaagaaa aacaatctcc agaatcccga
     9901 gaacaagcgc gagatcatgg cggacgacaa tctgcggccg atcttcggcg gccaggacaa
     9961 ggtcagcatg ttcgaaatga acaagcatct gtcgaagcat ctgagccagc cgtaacggcg
    10021 gccgatcgcg ccaacagcgg atcaagggcg agtcgccggc gcgactcgcc cttttttatt
    10081 gtctcgccgg tccccggtgc agcggaagca aaatcaacgg cagccccgcc agcccatcgc
    10141 gccgcgatcg gcctggtcga ggtgaagatg gtcgcggtga gcctcattgt aatcggggct
    10201 gagcacggtg gcgaacagcc cgcaggcgcc gtcgcggacc tcgctcagga aggcgccctt
    10261 ggcgccatca tcgtcccagt cgccgagcac gctcacccgc gagccgtcct cgagccggaa
    10321 gccggcaatg tcgaccgcat tggcggtggc atgctcgctc caggcaccct ccgatcggcc
    10381 atagagccgc cggcagctat agcttccgaa atggtcgatc gcgacgacct tcagcccgag
    10441 atgttcgagc gccgccgcct gcacgacatg ccactcccac agcgccagcg cggccgccac
    10501 cggacagctg gcgccgagcc cttccggccg gtagcgcaag gtgacggcgc cgccggtgag
    10561 ccgcaccgcg tcgctatagc cgcaatgctc cccgcttgag cgcggcggga gcggatcgaa
    10621 ccgcaccccc gcctgctcca gcagcgcttg gcactgcggc ccctcttcgc gcagcccgac
    10681 cagcttgcgg ccggtaaagg cgcccaccgg ggcggtgagg tcgatctcgg tccacggcaa
    10741 atcctcggga tggttgcgcg catagccgta gagcagcatc gcgccgaagc cgatcagggt
    10801 cagccagacc aaagtcctga aggcggagag caggcgcatc atgctccgga cgcgtgaagc
    10861 gccgccgggt tgcgctcagc tgcggcgcgc ggtctcgagc cgctcggcgg tcaccggata
    10921 gcccgccgct tcggggatgc acagccagcg ctcgccgccg cgttcctcga tccaggggac
    10981 gatcttcgca agcgggtgcc gcgccgcatc ggcccgcgct tcctcgatcg aggcgaagcg
    11041 ggcgaggcgt tcgaggttgc ggcgggtcgg gaagatgatg cgggcgcggc cggcgtccgc
    11101 ctccgccagc acctcgtccg cgctcgccca cagcatgcgc accgcctccg cctccgcgac
    11161 cggcgccgcg ggcgcggccg cgggcgcttc ggcgatgaag aacaaggtgt cgaaccgccg
    11221 cgcttccttg aaggtcggac accagcgcgc gaacggcgtc agcgccgcca aatcgaggcc
    11281 gagcccgaat ccctcgagca gcgcggagaa gggggtgcct tcctccagct cctggcggat
    11341 ctggctgatc gttgccggat cgggggcggg cagcaacgcg ggcgcgactc cggtctcctc
    11401 gatcgtctcg cgaaccgcgg cgacccgcgc cgccggatgg tcgaagccct ggccgatccg
    11461 ctccgcgagc cattcgtcat cagggtcgat ccgcccgccg gggaaaacca gcgccccggc
    11521 cgcgaaggcc atattgcggg tgcgctcgat catcagcagc tcgggagcgc cgcccgcgct
    11581 gggacgcatc agcaccaaag tggcggccgg gatcggatcg ggcagatcgt ccatcaccgc
    11641 ctcgtagcca cccccgccgg agaggcaagg gcgccgcccg aaagccgtct cgcagctatt
    11701 tgaatttcac ggttcccccc ccaacaattt tcgatataca taacctaagt ttgttggtga
    11761 gttcccgagt acccgggcga accacgtttt ctggctcctc cccggttcgg gccgtcgccg
    11821 cggtagggga gatgtatggc cgttgcggtt gcgatagctc tgctcgtgct ggcgtcgatc
    11881 gcctttcatt tcctcagccc gtggtggatg acgccgctcg cgtccaattg ggggtcgatc
    11941 gacgacgccc tgatcatcac cctctatatc tgcgcgatcg ccttcgtcgg cctcaacctg
    12001 ttcatggcct acgccttgct caaatatcgc caccgccccg ggcacagcgc ccattacgag
    12061 ccggaaaacg cccggctgga gcggcggctc accttctgga ccgggatcgg catcgccggc
    12121 atgctggcgc ccgggctgat cgcctggaac gaatatgtga cggtgcccaa ggaggcgact
    12181 gtgatcgagg cgaccggcca gcaatggcaa tgggcgttcc gcttccccgg ccaggacaac
    12241 gtgctcggca ccgccgccgt cgcccacatg accgccgaca atccattcgg cgtcaatccg
    12301 cgcgaccccc acggccgcga cgacgtgctg atcgaaacca acgagctcca catgccgatc
    12361 ggcaagccgg tcaagatggt gctgcggtcg aaggacgtgc tgcacgattt ctacgtgccg
    12421 cagttccgcg ccaagatgga tctggtgccc ggcatcgtca cctatttctg gatgacgccg
    12481 accaagctcg gcacttacga gatactctgc gccgaattgt gcggcgtcgg tcatcacgaa
    12541 atgcgcggca ccgtggtggt gcaggaccag caggcgttcg acgcctggat gggcgagcag
    12601 atgaccttcg ggcaattgct cgcgcagaac cagggcgaca aggccaccca gctggccagc
    12661 gccgacgtca aaccgaccat cggggggcgc tgaatggcga cgattaccca tcctacgctc
    12721 gcggacgacc tgacgccgct ccaccatccc aagacctgga tcggcaaata tgtctggagc
    12781 caggaccata aggtcatcgc catccaatat acgctgaccg cggtgtcgat cgggctggtc
    12841 gccttgttcc tgtcgatgct gatgcggctg cagctcgctt atcccggcag cttcgagttc
    12901 atccagcccg gcaattatct ccagtcggtc accatgcacg gcatgatcat ggtcgtgtac
    12961 ctgctcaccg ccctcctgct cggcggcttc ggcaattacc tcatcccgct gatggtgggc
    13021 gcgcgcgaca tggtgttccc gttcgtcaac atgctgagct actgggtcta cctcctgtcg
    13081 gtgatcgtgc tggcggcgag cttcttcgtg cccggcgggc cgaccggggc gggctggacg
    13141 ctctacccgc cgcagaccat cctcgaaggc acgcccggcc acgaatgggg catcgtcctg
    13201 atgctggcca gcctcgccat cttcatcgtc gccttcacca tgggcggcct caattacgtc
    13261 accaccgtgc tccaggcgcg gaccaagggc atgacgctga tgcgcatgcc ctgctcggtc
    13321 tggggcatct tcaccgcttc gatcatgggc ctgctcgcct tcccggccct gttcgtcgcc
    13381 gcgatcatga tgctgctcga tcggcttgcg gggaccagct tcttcatgcc ggcgatcctc
    13441 tcgatgggga aggatgccgg ctacgagggc ggctcgccga tcctgttcca gcatctgttc
    13501 tggttcttcg gccatcccga ggtctacatc gtcgctttgc cggcgttcgg catcgtctcc
    13561 gacctcatct ccgtccacgc ccgcaagaat ctgttcggct accgcatgat ggtgtgggcg
    13621 atcgtgttca tcggcgcgct cagcttcttc gtctgggcgc atcacatgta cgtgtcgggc
    13681 atgaacccct atttcggctt cttcttcgcc acctcgaccc tgatcatcgc gatcccgacc
    13741 gccatcaagg tctacaattg gctgctcacc ctgtggcgcg gcgacatcca tttgcgggtg
    13801 ccgatgctgt tcgcgatcgg cttcctgttc accttcatcc atgggggcct caccgggctg
    13861 ttcctcggca atgtcagcgt cgacgtgccg ctgtccgaca ccttcttcgt gatcggccac
    13921 ttccacatgg tgatgggcgt ctcgccgatc ctggtcatct tcggcgccat ctatcattgg
    13981 tatccgaaga tcaccggccg gatgtggaac gacgccgtcg cgaagtggca tttctggatc
    14041 accttcgtcg gtgtctacgc gatcttcctg ccgatgcatt atctcggctt cctcggcgtg
    14101 ccgcggcgtt attatgcgct cggcgagacc gccttcatcc cgccctcggc gatggtcgcc
    14161 aaccagatga tcaccatcgc ggccttgatc gtgggcgtgg cgcaattgct gttcttctgg
    14221 aacatggcct ggagcctgtt ccggggcgag aaggcgccgc ccaacccgtg gggcgcggcc
    14281 tcgctcgaat ggcagacgcc cgatacgccg ccagcgcacg gcaattgggg gccgagcctg
    14341 ccggtggtcc accgctgggc ctatgattac agcgttcccg ggtatccgcg cgatttcatc
    14401 ccccagaacg agccgcacga tccggccatg aagcggcacc acgaaacatg agcatcttct
    14461 cccgcctcgc cgaaaagagc tggctgacgc ccggcgccga ttatgacgat ccgaccggct
    14521 accggccctc ggccggccgc gtcgcgatgg tcgtctattt cgtcgtcgcc accgtcatct
    14581 tcggcctcgt ctcctcggcc tatgtgatgc ggatgggcat gcccggcatg ggccacgggc
    14641 cggtcggcga ctggcggccg atgccggagc cgcctttgct ctgggtcaat actggcatcc
    14701 tgctcctcgc cagcctcgcc tgggaggcgg cgcggagcgc gctccgccgc ggccgggtcg
    14761 atggggcgcg cggcgcgctg atcggcgccg ggctgctcgg gctggccttc ctcgccgggc
    14821 agctgctgct ctggcggcaa tatggcgagg ccggctatta tctgcagtcg aacccggcca
    14881 attccttctt ctacctgatc accggcctcc acggcgcgca cctgatcgcc ggtttgttct
    14941 tctggctgcg cgcctacgcc cgcgccggcg acaggaccga tgccttcgcc gccggccagg
    15001 cggtcgatgt cagcgcggct tattggcatt tcctgcttct ggtctggctc gtcatgttgg
    15061 gattgctcgt atcgacctga actaacggga gtagatgatg tccaccgtga tccatcaggg
    15121 cgagaatgcc ggagggccgc cgcagcaatt gcgcgagttc gccagcgact ggagcacgga
    15181 tcaggaagtc ttcaagcagg tccactgggg caaggccatg atgtggatct tcctcctctc
    15241 cgacaccttc atcttctcct gcttcctcac ttcctacatg accatgcgcg cctcggtgac
    15301 ggtgccgtgg cccaacccgt ccgaagtctt cgcgctcacc atcgccggcg aatcgatccc
    15361 tttgatcctg atcgcgatca tgaccttcgt gctgatcagc tcgtcgggca cgatggcgat
    15421 ggcggtcaat ttcggctatc gcggcgaccg ccggacgacc gcctttttga tgttcgccac
    15481 ggcccttttc ggcctcacct tcgtcggcat gcaggcgttc gaatggacca agctcatcca
    15541 cgagggcgtc cgcccgtgga gcaatccctt cggcgcggaa cagttcggcg cctccttctt
    15601 catgatcacc ggctttcacg ggctccacgt ctcggccggc gtgatcatgc tgttcgtggt
    15661 cgccttcaag gtgctgcgcg gccagtatga gcgatccggc gactattccg cggtcgagat
    15721 agccggcctc tactggcact tcgtcgatct cgtctgggtg ttcattttcg cattcttcta
    15781 tctctggtga ggccgcagcg atgaaccatg gtaccgacac cgcgcacgct ccggccgccc
    15841 atggcgccgg ccatggcgtc gcccatggcg ccggccatgc cgccccccgc gcggcgcccg
    15901 cacacgatcc cgggcagcag catccgctgg gcgtctattt caaggtctgg ggcctgctgt
    15961 tcgtgctgag cgcgctctcc tacgcggtcg attatttccg ggccgaaggg atcatgcgct
    16021 gggtgctgat cctcatcttc atgttcctga aggccgggct gatcatcgcc atcttcatgc
    16081 atctggcctg ggagcggatg gcgctgatct acgccatcct ggtgccgccg ctcgcgctca
    16141 tcaccctggt ctggctgatg ctgatcgaat ccgattacac tttctggacc cggatcctgt
    16201 tcttcgacgg gggtggctga tggacggttg ggaatggacc aagctaggcg gcgcgctcgg
    16261 cggcgcgctg gcggtggcgc tcggcggctg gtggttcgcc ggcctggtca tgtatccggc
    16321 ctacccggaa gggcgcggct accaggtcga gggcgtggcc ccggtcgatc tcgccgccat
    16381 gcagcgcagc tggccggccg gcatgcagca gcccgacgat cccaccgagc tgcgcggcta
    16441 tatggccaat atcgaaaagg cggtgctgcc cgcgccggcg acgggggcgg gcccgctcgc
    16501 gccggccgcg ccggtcgatc tcggcacctt gctggcggcg gccgatccgg cgcgcggcaa
    16561 gggcacggcg cgcacctgcg gcagctgcca cacgttcgag caaggcgagg cggaccgggt
    16621 cgggccgaac ctgtgggcgg tggtcggccg gccgatcggc ggcaagagcg gctacggcta
    16681 ttcggcggcg atcgcggccc atggcggcgc ctggacctat caggagctcg acgcctatct
    16741 ggccagcccg ggcaaggcga tcccgggcaa caagatggcg ttcaacggca tccgcaaccc
    16801 caaggaccgg gccaatctgc tcgcctatct cggcaccctc aacgccaccc cgtcgccctt
    16861 cccggcggcg aagcccgccg gtacccccga gggcgccgct tccaccaccg gcgcgggagc
    16921 cgttccgacc agccgttgaa gctcagtcgg cgtaccggtc ggcgaaggcg gcggcggcgc
    16981 cgtacaggcc cggctgctcg tgggtgacga tccggaccgg catccgctcc atccgctgcc
    17041 tgtaccgccc tttgtcgaca aagcgctcat ggaaggccga gttcggcagg cgatcggcca
    17101 gccgcagccc gagcccgccg ccgatcgcca ccgccgccgc gccctgcgcc agcgcgatat
    17161 cgcccgcggc cgcgccgagc gccccgcaaa agcgctccag cgcctccccg gccagcgcgt
    17221 cctcgccggc cagagccgcc gcccacagtt ccttgtcctc ggcataggca ggcgtccgcc
    17281 ccccggccga ggccagcgcg ctgtaaatga cggcgaggcc ggctccggag acgatccgct
    17341 cgaccgagac ccggccgaag cgggggcgca gcccggcggc cagcgcgtcc tcgaaagcat
    17401 cggcgggggc gaacccgata tgcccgccct cggtctcgat cacctccgcc cgccccccgc
    17461 gccggagcag ttgcgcgacg cccagccccg tgcccggccc gacgatgctg atcagcccct
    17521 cctccggaag cggccgatcg ggtccgcaaa ggtggcggaa atgcaccgga tcgagctgcg
    17581 cgaccgcatg ggccacggca ccgaaatcgt tgacgaggcg aagccgcgac acgcccagcc
    17641 gggtcgggag accggtgcgg tcaatcgtcc acggattgtt ggtgagcttg atcgtatcgc
    17701 cctcgatcgg cgccgcgacc gcgatcgccg ccgccgccgg aagcggccgc ccggtccgcc
    17761 gcgcaaactc cgcccaggcg tcttcgagcc cggcatggtc ggcggtcgcc agcgtgcacg
    17821 gctcgcccaa agcggcgacg cggccgtcca ctacctccgc cagcgcgaag cgggcatggg
    17881 ttccgccgat atcggcggcg acgatctcgg tcatgaggcg gcgtccatcg cggccagcat
    17941 agcgcccgcc gggcggccgg tcacggctcg ctaatgaact cgggcggcac ggagcgggta
    18001 agccacaccc cattgtccgc gcgatagaat atatgaccgg ccgcgtgcat cgcgcctgaa
    18061 tccacccgca acaccatcgg cttgccgtgc ctgcgcccga cctgcaccgc cgtcgccgcg
    18121 tcgggcgaca gatggacata gtggcgcttg cgcggttcga gcccgctgct caggatcgac
    18181 ggcaggttgg tttcggcggt gccgtggtag agcagggccg gcggcgccat cggagcgaag
    18241 gcgatctcga cctgcggggt cgaatggccc tgcgccgccc gaatgcgggc gccgtcctcc
    18301 gacagggtga agcgcttctt gtcgctgttc gcgacgaccg cgccgagcct tgcctcgtcg
    18361 acctcgaagc ccttccgccg cgctgcctcc agcacctgcg cgacctcggc ccagccttcc
    18421 ctgtccagcg aaatgccggc ggcttcgggc gaatgacgca gaacgtagct caggaacttg
    18481 ctcagcctga tcgcgtccct gggagccttc atgaccatcg tctggcgagg cggcggccat
    18541 cccgcaagcc gccgctttcc tacacgcctc ctatttgtca tcctcgccaa tggccgattc
    18601 gttcagccgc cgctctttga acttgccgat gccctccgcg cctccgcgcc tccgcgtgag
    18661 catgacgccg tctgcgtccc gaacggcttc acgaaacctg gagaagacgg tgcgcttcgc
    18721 ctcgccgcgc cgcctgttcg acgtcactgc gcaagccggt gctcgtgtcg cggtccaatc
    18781 gaaatcgcac tcggttcgcc gcaatttcgc cggtacgtag cgccaccttt gtcaccttcg
    18841 cccatttccc cccggcgtcg atcaggcccg gcactggcct ccgaccgtcc cggcacagcc
    18901 agagcttcgc cgcgcgcgag gcgcttcgcc ttagccgtgg atagagggtg gagatatggt
    18961 ggagccgagg gggatcgaac ccctgacctc tgcagtgcga ttgcagcgct ctcccagctg
    19021 agctacggcc ccgcgccctt tggggaacac catccgattg gcatggcacc cacggcgaag
    19081 gcgcctccaa tagccgcgct cgggggccgg cgcaatgcgg caaattggcc cgcccgcgag
    19141 cggggtcgtt ccgccgccga gccgcaacgc actgcctctc cggccggaac gcgccgctct
    19201 tgcgcatcgt tttaaccctc ggatcgcgag gcgcgcggat cggctgcgcc cctgtactgc
    19261 gaccccacca acctgaagga ggatttcatg ggctatggcg atgaccgata tggcggcggc
    19321 aaccgctccg gcggcgacga ccgatggcgc gatcgcggcc agggcgggaa ctggcgcggc
    19381 gagcgcggcg gctttggcgg cggccggccc gagggcgacc aggaccgcgg cttcttcgag
    19441 cgcgccggcg agcaggtccg ctcctggttc agcgacgatg acgacgatcg cggtcgcggc
    19501 ggcggcaact attcgcgtgg cggcggcggc ggcggctcgg gcagcttcgg cggcggctgg
    19561 ggcaaccagc agggcgaaag ctggaaccgc gaccggccgg gcatgagctc gggcggcggc
    19621 tacggcgccg gcaaccgcga ctatggcaat gactggggca gcggctccgg cggcggcggc
    19681 ggctccggca gcggctccgg cggctatgcc cgcgactatc aatcctccgg cggcggcagc
    19741 gatcgcttcg aggaaggcag cttcggccag gaggcgcggt cgagcgaggt ctggggcggc
    19801 tccggcttcg gcggcggcac cgatcatggc cggcgcttcg accgggtcga tccgggcgcg
    19861 gtcggcaccc atggcgccca cccgatgtcc tcgccggtcg gcggcgctta cggtgccggc
    19921 tacggcgcat cgatgggcgg cggctatggc agctcggcgc gcaccgcggc gatgctcggc
    19981 ggcggcggcc gcggcgcgac ttcgggctat ggcagctcgg gcgcttccta tgcgggcccg
    20041 caaggtggcc ggcagggcgg cggccagcag ggcggtggcc agcaaggcgg cttcggcggc
    20101 ggcggccggc cgggcatcca cgacccgcat tattcggaat ggcgcagccg gcagatcgag
    20161 cagctcgacc gcgactatga cgaatatcgg cgcgagcatc agtccaagtt cgagcaggag
    20221 ttcggcggct ggcgccagaa gcggcagggc cagcgctcct cgctcggccg catcaccgag
    20281 catatggaag tggtcggttc ggacggccag cgcatcggcg tggtcgacaa ggtcggcggc
    20341 gaccgcatca tcctcaccaa gaatgatgag aatgccggcg gccgccacca ttccatcccc
    20401 tgctcgtgga tcgagaatgt cgacgagcgc gtgacggtca acaagaccgc ccaggatgcg
    20461 atgaacgcct ggcgcgacga ggagcagaac cgcgccctgt tcgaacgcga ggatcaggga
    20521 agcgagggtc cgcacatgct gaaccgcagc ttctcgggca cctatagcga ccgcgacgac
    20581 taaacgccgc gtccctcaag gctcccttgg cccggccggt tttccccttt tccggccggg
    20641 cctttttttg actctccccc gcattacggc aaaagccgga gccgcgctga ggccgggctt
    20701 ctccggcagc acggagggag acttacatga ccggcacacg cgcctggcac ctcgcatccc
    20761 ggccttccgg catgcccgac atgaccaatt tcgagcttcg cgaaatggag ctgccgccgc
    20821 tcgaggacgg catggtgcgg gtcgagaata tatggctctc ggtcgatccc tatatgcgcg
    20881 ggcgcatgaa cgacgtgaaa agctatgtcc cgccgttcga ggtcggagcc ccgctgcaag
    20941 gcggcgccgt cggcaaggtg gtcgagaccc gctcgccgca cttcaacgtg ggcgacaccg
    21001 tcttccacat gctcggctgg cgcgagcagg cggtggcccc ggcggcgcag ttcaacaagg
    21061 tgccggcgat gggcgtcccc gatccgcaat ggctcggcaa tctcggcctc accggcgcca
    21121 ccgcctattt cgggctcctc cgtgtcgccg aggcgcggga gggcgaaatc gtgttcgtgt
    21181 ccgccgcggc gggcgcggtc ggctcggcgg tggtccagat cgccaaggcc agaggcatga
    21241 ctgtgatcgg atcggccggc ggcgaggaaa aatgcgcctg ggtgcgcgag ctcggcgccg
    21301 acgccgccat cgactataaa gccgggccgg tggtgaaggc actgaccgcc gcggcgccga
    21361 aagggatcga cgtctatttc gacaatgtcg ggggcgagca tctcgacgcc gccttcgccg
    21421 ccgcgcgcaa ccgcgcccgc ttcgcgattt gcgggatgat cgacaattac aatgccggcc
    21481 cgccgccaag cttccgctac atcatgcgtg tgatcgccgc ccgcatccag ctcaggggct
    21541 tcatctacac cgactatctc ccggaaatgg gcgacttcta ccgcgacatg ggtggctgga
    21601 ttgcaagcgg ccaggtccag agccgcgata cggtgcgcga ggggatcgag gcgacgccgc
    21661 aagccttcct cgatctgttc acgggcggca acatgggcaa gatgctggtc cggctctagc
    21721 gggaggcgcg tcatttgacg atggtgaggc cgcgcctcat ttcgccgctg cgcacgtcga
    21781 gcaccgacgc gccgacatag gcgaccacct tctccggccg ctctgccggc tgcgccgccg
    21841 ccattgctcc cgagaccagc gccaggatcg ccgccgccca gcgccgccac cccgttcgaa
    21901 ccatccgcct tcctcccggc cctcttcatg gcacagcaga gccgctgccg gaacggcccc
    21961 gatgctggcg gcggcggcgg cgagcggtta gacagcggcc atggccaggc ccatcacctt
    22021 catcgtcgcc cgcgccgaca atggcgtgat cggcaaagac ggcacgctgc cctggcacct
    22081 gccggccgac ctcaagcggt tcaagcggct gaccatgggc agcgcgatgg cgatggggcg
    22141 tcggacgttc gacagcctgc ccggcctgct gcccggccgc cgccacatcg tcctcacccg
    22201 cacccccggc tggagcgcgc ccggcgcgga gccggtccac tcggtcaagg aagccatcgc
    22261 ccgcgccggg gcccacccgc tggcggtgat cggcggcgcc gagatcttcg ccttgttcga
    22321 acccgcggcc gatgcggtcg agctcaccga agtccatacc gacgccgaag gcgacacatt
    22381 gatggccccg ttcgacccag cccgatggcg cgaagtgttt cgcgagccgc accccgccga
    22441 agaaggtcgg ccggcctaca gcttcgtccg gctggagcgg aacggatgat gcggcgggtc
    22501 gcgctcggcc tgctgctgct gctcgtggtc gccgtggccg gcctgctcct gatcggcccc
    22561 gggatggtcg agaagagcct caacacgatc gtggagcagg agcttcccgc gccctcgccc
    22621 gccgtccagg cgctgcaccg ccggctcgcc atcgccgacc ttcatgccga caccttgctc
    22681 tggcagcgca gcctgctcga cccggcgacg cgcggccatg tcgacctgcc gcggctcgag
    22741 gccggtaacg tcgcgctcca gatcttctcc tcggtgacca aatcgcccaa ggggctgaat
    22801 tacgaccgca acgccgccga cagcgacgac attaccgcgc tcgccctcgt ccagctgcag
    22861 ccgccgcgga cctggtcctc gctcgccgag cgctcgctat ggcattccgc caagctccac
    22921 cgcgccgagg cggcctcggg cggccggctg cgcatcgtcc gctccgccgc cgatgtccgg
    22981 cgcctcatcg ccgaccgcgc ggccggcaag ccggtgacgg gcgcgatgct ctcggtcgag
    23041 ggcctccaca atctcgaagg caagctcgcc aatctcgccc gcctcgacgc cgccggcttc
    23101 cgcatggcgg gcctggtcca ctttttcgac aatgaggtcg gcggctcgat gcacggcctc
    23161 gccaaaggcg ggctgacgcc gttcgggcgc caggtggtgc gggcgatgga ggcgcgcggc
    23221 atgatcgtcg acctggccca tgccagcccg gcgacggtgc gcgacgtgct cgccatggcg
    23281 cgccggccaa tcgtctccag ccatggcggc gtacaggcga cctgcaaggt caaccgcaac
    23341 ctcaccgacg aacaggtccg cggcatcgcc gccaccggcg ggctcgtggg gatcggctat
    23401 tgggacggcg ccgtctgctc ggcggacccg gccgccatcg ccgccgccat cgcccatgtg
    23461 cgcggcctgg tcggcatcgg ccatgtcgcg ctcggctcgg atttcgacgg ctcggtgacg
    23521 accggcttcg acacgtcgca gctggtccag gtcacccggg cgctggccgc gcgcggcttc
    23581 accgaagccg agatcgccgc cgtcatgggc ggcaatttgc tgcgcttgct cgaacgggcc
    23641 ttgccgcaac gatgacgttc ccccggtgaa ggcccgggcc cagaacgatg acgttccccg
    23701 gcgaaggccg gggcccagac tccctcctgg accccggctt tcgccgggga acgtcagcct
    23761 gcgccaccca tgttggcttg gcggccccgc tctcctatag cgcggccatg gagcggctgg
    23821 agggcggctc ggcggttccg gaccatttgc gtggcggcat cgtcgcgctc ggcaatttcg
    23881 atggatttca cgcgggccac caggcggtgg tcgggcgcgc ggtcgaacgc gcccgcgccg
    23941 aacgccggcc ggtgctggtc gccaccttcg atccgcatcc ggtgcgcttc ttcaagccgg
    24001 acctgccgcc gttccgcctg accacgctcg accagcgcga gcgcctgttc ggggccgccg
    24061 gcgccgacgc catgatcgtg ttccgcttcg atgccgagct tgccgcgacc agcgccgagg
    24121 atttcgtcgc cggccgcctc gccggccgga tcggcgccgc cgggctggtg accggcgagg
    24181 atttcacctt cggcaagggc cgctccggca atatcgaagt gctgcggagc ctcggcgccg
    24241 cacacggcct cagcgtcgat gcggtcgcgc cggtcgcggc cgacggcgag ccggtttcgt
    24301 cgagccgaat tcgcgacgcg ctccaggccg gcgactgcga gacggccgcg cgcctgctca
    24361 cccgcccgtt cgcgatcgaa ggcgtggtcg agcatggcga caagcgcggc cgcgaactcg
    24421 gctaccccac cgccaatctc gccctcggca aatatctgag gcccagatac ggcgtctacg
    24481 ccgtgcgcgg gcggctcgcc gacggccgcg tcctggccgg cgccgccaat ctcggcatcc
    24541 gccctacctt ccagccgccg cgagagctgc tcgaacccta tttcttcgac ttttccggcg
    24601 acctttacgg ccagccgctc gaggtcgagc tcatcgcctt ccttcgcccc gaacgaaggt
    24661 tcgaccgcct cgataccctc accgcccaga tggacgccga ctgcgtcgaa gcccgccgga
    24721 gactgaccgc gtgaaaatag ccaaatgggc cctcatcctg atcgccctcg ccgccgtcat
    24781 cctgtcgctc accaacgcct cgtggatcgc gcccaagccg acgggcaagc tgatcctcgt
    24841 cgcccatcgc ggcaccggcc agccggccgc ggccggggca agcggggact gccccggcgt
    24901 cgccatcacc gcctccggcc acaattatct cgagaacagc gtccacggca tgaaggccgc
    24961 cgccgctttc ggcggcgacg cgctcgccct gcccgtccag cgcaccagcg acggcttgat
    25021 cgttatcttc agcgacccgg cgctcgactg ccgcaccgac gtcagcggcc cggttgccgc
    25081 gcgcagcctg gccgaattga agcggctcga cgccggccac gattacacgc ccgacggcgg
    25141 ccgcaccttc ccggtgcgcg gccgcatcgg cggcatccag accctcgacg aagtgctgtc
    25201 ggagatgcgc ggccagcggt tcatcttccg cttcgtcggc gacgatcccg ccgccgccga
    25261 cgcgctcgcc gccaccttcg agcgcgccgg cgccaagatc gacccgggca ccggcttcct
    25321 cggcccgaag gccgccaccg accgcatgaa acagctcgcg cccgaggcct gggtctacga
    25381 tcccgcgcaa agccgggcct gcctcgccgg ctatgcccgc accggctggg ccggcatcat
    25441 cccgcccgaa tgccgcgacg ccgtcctggt agtgccctat gacagcggct ggaccatctg
    25501 gggctggccc taccgcttcc tcgaccgcat ggccggcgtc ggcgccgagg tgatgctggt
    25561 cgcctccctc gacgcgaacg gccacccgct cggcctcacc gagccgcaac agctcggcga
    25621 cgtcccccgc cactttcgcg gcatgctctg ggtcgagaac atgtacgagg tcgggcgcgc
    25681 gctgaattga cggcgggtgt tcccttccca ccgcatcccc gctaatcccc gccgcgatgt
    25741 ccgaaaagcc cgattacaaa gacacggtct tcctccccaa gaccgatttc ccgatgaaag
    25801 ccggcctcgc gcagaaggag ccggctatcc tcgcccgctg ggctgagacc gggctctacg
    25861 agcagatccg cgccgcccgc gccggcgccg agcgcttcat cctccacgac ggcccgcctt
    25921 acgccaatgg cgacatccac atcggccacg cgatgatgaa gaccatcaag gacttcatcc
    25981 tccgctcgca gtcgctgatg ggcaaggacg cgccgttcat ccccggctgg gactgccacg
    26041 ggctgccgat cgagtggaag atcgaggagc agtataggaa gcagaagaag gacaaggacg
    26101 aagtcccgcc gcgcgaattc cgcgccgaat gccgcgctta tgccgagcgc tgggtcgagg
    26161 tgcagtccgc ccagttccag cggctcggca tcctcggcga ctgggccgac ccctatcgga
    26221 cgatggacta tgcctccgaa gcggtgatcg ccggcgagct cctgaaattc gccatggcca
    26281 gcaagctcta ccgcggcgcc aagccggtca tgtggagccc ggtcgagaag accgcgctgg
    26341 ccgaggccga agtcgaatat gaggacatcg actcgaccca gatcgacgtg gcgttcgaga
    26401 tcgtggagag tccgatcccg gagctggtcg gcgcccatgc ggtgatctgg accaccacgc
    26461 cctggacgat cccggtcaac caggcgatcg cctacgggcc ggacattgaa tacgttctct
    26521 gggccaatcc cgttggcggg gcagagtgga ttggctccgg tagcgagatg ccgctggcgt
    26581 cggttcgcta cctgctaatc gcggaacccc tgctgctagc gttcctcgat cgaaccaatg
    26641 gagggaatgc cagaccgcca tcggctggac tggaatggta tggagatcca gtgccgccat
    26701 tcacgtggcg cggcaaaggc ttcgacctcg ccggcaccgt cgcgcgccac ccgatgcacc
    26761 atctcggcgg cttcttcgcc cgcccgcgcc ccttcctccc aggcgatttc gtcaccaccg
    26821 acgccggcac cggcctcgtc cacatggcgc ccgaccatgg cgaggacgat ttcctgcttt
    26881 gcaaggcgca cggcatcgag ccggtgttcg cggtcgaggc ggacggcaag taccgggcgg
    26941 actgggaatg gctcggcggc cagggctcgg tcatcaaccc caagttcaac gcgcccgacg
    27001 gcccgatcaa ttcggacctc cgcgaagccg gcgccctcgt cgccgccagc gccgacttcc
    27061 ggcacagcta cccgcacagc tggcgctcca aggccaagct catcttccgc gccaccccgc
    27121 aatggttcat cccgatggat caaagcccct cgcccccttc tgggggggag ggacagccgg
    27181 cgcgaagcgg cggcagggag aggggggagt ccggtgggct gtcgtcgggc ggggcccccc
    27241 cccccccccc cccccccccc cc
//
LOCUS       DASVRK010000285         6356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_121100, whole genome shotgun sequence.
ACCESSION   DASVRK010000285 DASVRK010000000
VERSION     DASVRK010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121100"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2396)
                     /locus_tag="VF589_11805"
     CDS             complement(<1..2396)
                     /locus_tag="VF589_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8258307.1"
                     /translation="MNVESPRDFLDRRQLVSALRSLRRGDFTVRLSEDLPGLDSEVAR
                     LFNEVVTLEEQITEEFERLSRVVGKEGKITQRGRVRGATGSWDAKLRAVNELIEDMVQ
                     PTAEVSRVIGAVAKGDLSQSMTVEIDGRPLRGEFLRIGKVVNTMVEQLASFASEVTRV
                     AREVGTEGKLGGQAMVKGVAGTWKDLTDNVNAMATNLTGQVRNIAEVTTAVASGDLSK
                     KITVEVKGEILELKNTINTMVDQLNSFASEVTRVAREVGTEGKLGGQARVEGVAGTWK
                     DLTDNVNLMADNLTGQVRNIADVTTAVAKGDLSKKITVDVKGEILELKNTINTMVDQL
                     NGFASEVTRVAREVGTEGKLGGQAQVEGVAGTWADLTDSVNRMAGNLTGQVRNIAEVT
                     TAVASGDLSKKITVDVKGEILELKNTINVMVDQLNGFASEVTRVAREVGTEGKLGGQA
                     QVPGVAGTWADLTDNVNLMAANLTGQVRNIAEVTTAVARGDLSKKITVEVKGEILELK
                     NTINTMVDQLNGFASEVTRVAREVGSEGKLGGQAQVEGVAGTWKDLTDNVNLMAGNLT
                     GQVRNIAEVTTAVARGDLSKKITVDVKGEILELKNTINVMVDQLNSFASEVTRVAREV
                     GSEGKLGGQAQVPGVGGTWKDLTDNVNAMAANLTGQVRNIAEVTTAVALGDLSKKITV
                     DVKGEILELKNTINTMVDQLNGFASEVTRVAREVGTEGKLGGQAQVRGVAGTWKDLTD
                     NVNLMAENLTGQVRNIADVTTAVARGDLSKKITVDVKGEILALKDTINVMVDQLNGFA
                     S"
     gene            2610..2981
                     /locus_tag="VF589_11810"
     CDS             2610..2981
                     /locus_tag="VF589_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258308.1"
                     /translation="MASDQEISGFIRATFRSIWALELLLFLRKNGDRSWSHAEMVASL
                     RGSDLVVAQSIEALSAAGLVLAEEGGSARYQPVSEDLDRLVAGAGEMYARSPDAVRRM
                     IVASAHGGLAAFADAFRLRKD"
     gene            2985..3260
                     /locus_tag="VF589_11815"
     CDS             2985..3260
                     /locus_tag="VF589_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX8258309.1"
                     /translation="MAQLFPAAVYLLCFATSAACAFLLARNYARTRARLLLWSALCFV
                     LLAANNLVVVIDLLLLPQMDFRLVRLLLSLSGVSVLLFGFIWDLEED"
     gene            3265..3540
                     /locus_tag="VF589_11820"
     CDS             3265..3540
                     /locus_tag="VF589_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX8258310.1"
                     /translation="MTIYDFLSGAVTMGFVLAGLFFLRFWRRTHDGLFLAFALAFWLL
                     GLTQALLSFSNIPVEERSWVYLFRLAAFALILASIGLKNFRARSGQR"
     gene            3622..4173
                     /locus_tag="VF589_11825"
     CDS             3622..4173
                     /locus_tag="VF589_11825"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX8258311.1"
                     /translation="MEPLDQNGRAITDKSDRDRMLAELYDELRAAASRLLRREAPFLT
                     LEPTDLVNEAALRVMKLDSMSWHDRQHFFATGARILRQAMIDAIRRRRSAKRRMPTLM
                     FADEEGSDIDVEALDEALTRLEVASPELARIVELRFFVGLGVTEIAAVTDSSESTVKR
                     RWRTARLWLAAELQPDAPQAPDR"
     gene            complement(4155..>6356)
                     /locus_tag="VF589_11830"
     CDS             complement(4155..>6356)
                     /locus_tag="VF589_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8258312.1"
                     /translation="TGTIGSGGMGTVVAAERDDGVYRQKVAIKLIRAGLKDARAQERF
                     ALERRILARLSHPGIARILDGGSADGRPYLVMEYVEGRPVTVDTERRRLGLAQTLDRF
                     AAVCEAVSHAHRNLVVHADIKPSNVIAAEDGSIKLVDFGIARLTAELDEDEIEGPYPL
                     TKGYAAPERLAGQPPTVASDVYSLGALLHEMLSGHRPDGRAMSEVAAEAPRRFGPRQL
                     AGELDAIVAKALAEDPARRYPDVASLAADLGSFCDKRPISAMRPTLAYRSRRFLARNR
                     LPLAIGGAVFILLAAAAIVSTSLYFDARRQRAAAEARFEEVRSLAKFQLFTLYGQLAA
                     LPGTLPARERLTREAQLYLDRLAALPDASAAVKLETAMGYNRLAEVQGVPNSPNLGRI
                     EAAKRNLGKAAAMLVPLASVRPANPAALRELARARLMQADMAIWQDNATATGERLLGA
                     AEPLVAAGRDGSAEWRALENQRRTVRLDVLGWSERYAEESAFASDTIGWLERLPAAER
                     RLPAYIIARAEAHNARAEGDWYRDRRREALLDAREADRLVREALARHPGRPELLSARL
                     ASGYNLITSLEPLGLEDGILEMALDVLAVARRLRAIEPNDRMIAWRIRNTEALAAQLL
                     SDAGRHGEAVAIQAGIVESEERRLAAAPADTRTARDLAFSRKVLGNLHWTAGRAGRAC
                     AIWRSSEAAFARLEQAGTLNEWDKSNVVAGLRGNLAICRGEAPASTYRSGA"
BASE COUNT          898 a         2216 c         2110 g         1132 t
ORIGIN      
        1 gaggcgaagc cgttcagctg atcgaccatg acgttgatcg tgtccttgag cgcgaggatc
       61 tcgcccttca cgtcgacggt gatcttcttg gaaaggtcgc ccctcgccac ggcggtggtc
      121 acgtcggcga tgttgcggac ctggccggtc agattctcgg ccatcagatt gacgttgtcg
      181 gtgaggtcct tccaggtgcc ggcgacgccg cgcacctgcg cctggccgcc caatttgccc
      241 tcggtgccga cctcgcgggc cacgcgggtc acttcggagg cgaagccgtt gagctgatcg
      301 accatcgtgt tgatggtgtt cttgagttcg agaatctcgc ccttcacgtc gaccgtgatc
      361 ttcttggaca agtcgccgag cgccaccgcg gtcgtcactt cggcgatgtt gcggacctgg
      421 ccggtaaggt tcgcggccat ggcgttcaca ttgtcggtca aatccttcca ggtaccgccc
      481 acgccgggca cctgcgcctg gccgccgagc ttgccttccg aacccacttc gcgggccacg
      541 cgcgtcactt ccgacgcgaa cgaattgagc tggtccacca tcacgttgat ggtgttcttg
      601 agctccagga tctcgccctt cacgtcgacg gtgatcttct tcgacaagtc gccgcgagcg
      661 accgcggtgg tcacttcggc gatgttgcgg acctggccgg tgaggttgcc ggccatcaaa
      721 ttgacgttgt cggtcagatc cttccatgta ccggcgacgc cctcgacctg cgcctggccg
      781 ccgagcttgc cttccgagcc gacctcgcgc gccacgcgcg tcacttccga cgcgaagccg
      841 ttgagctgat ccaccatggt gttgatggtg ttcttgagct ccaggatctc gcccttcacc
      901 tcgacggtga tcttcttcga caaatcgccg cgggcgacgg cggtggtcac ttcggcgatg
      961 ttgcggacct ggccggtcag gttggcggcc atcaggttga cgttgtcggt aaggtccgcc
     1021 caggtgccgg cgacgccggg cacctgcgcc tggccgccca atttgccctc ggtgccgacc
     1081 tcgcgcgcca cgcgcgtcac ttccgaggcg aagccgttga gctgatcgac catgacgttg
     1141 atcgtgttct tgagctcgag aatctcgccc ttgacgtcga cggtgatctt cttggaaagg
     1201 tcgccggagg ccaccgcggt cgtcacctcg gcgatattgc ggacctggcc ggtgaggttg
     1261 ccggccatgc ggttcaccga gtcggtaagg tcggcccagg tgccggcgac gccctcgacc
     1321 tgcgcctgac cgccgagctt gccttcggtg ccgacctcgc gcgccacgcg cgtcacttcc
     1381 gaggcgaagc cgttcagctg atcgaccatc gtgttgatgg tgttcttaag ctcgaggatc
     1441 tcgcccttca cgtcgacggt gatcttcttg gacaagtcgc ccttcgccac cgcggtcgtc
     1501 acgtcggcga tgttgcggac ctggccggtg aggttgtcgg ccatcagatt gacgttgtcg
     1561 gtcaaatcct tccaggtgcc ggcgacgcct tccacgcgcg cctggccgcc gagcttgccc
     1621 tcggtgccga cctctctcgc cacgcgggtc acctcggagg cgaacgaatt gagctggtcc
     1681 accatcgtgt tgatggtgtt cttgagctcg aggatctcgc ccttcacctc gacggtgatc
     1741 ttcttggaca agtcgccgct cgccacggcg gtcgtgacct cggcgatgtt gcggacctgg
     1801 ccggtcaggt tggtcgccat cgcgttgacg ttgtcggtaa ggtccttcca ggtgccggcg
     1861 acgcccttca ccatcgcctg gccgcccaat ttgccctcgg tgccgacctc gcgcgccacg
     1921 cgcgtcactt cggacgcgaa cgaggcgagc tgctcgacca tggtgttcac gaccttgccg
     1981 atgcgcagga actcgccgcg cagcggccgg ccgtcgatct cgacggtcat cgattgcgac
     2041 aggtcgccct ttgccaccgc gccgatcacg cggctgactt cggcggtcgg ctggaccatg
     2101 tcctcgatca gctcgttcac cgcccggagc ttggcgtccc agctgccggt ggcgccgcgc
     2161 acccgcccgc gctgggtgat cttgccttcc ttgccgacga cccgcgacag ccgctcgaac
     2221 tcctcggtga tctgctcttc gagcgtcacc acctcgttga acagccgcgc aacctcgctg
     2281 tcgagcccgg gcaggtcttc cgataggcgc acggtgaaat cgccgcggcg caggctccgg
     2341 agagcgctga cgagctggcg gcggtcgagg aaatcgcgag gggattcgac gttcacgtaa
     2401 cgctccttag tctctctccc cccacctcaa ctcaccggcc ccccagccgg aacgggcgca
     2461 ccatagcaga cgcaaacggc aacgcttctg aaaaaaagcc ctttgaggca aaagcctgaa
     2521 catggggttg ccagcgctcg accgaccgtt agaaggcccc gcagcacgca tcagcggcgg
     2581 atcgcagccg cggggaccca ccggcgaaaa tggcttcaga ccaggagatt tctggcttca
     2641 tcagggcgac ctttaggtcg atctgggcgc tggaactcct gctcttcctg aggaagaatg
     2701 gcgaccggag ctggtcgcac gccgaaatgg tggcgagcct cagagggagc gatctggtgg
     2761 tggcgcaaag catcgaggcg ctgagcgcgg caggcctggt gctggccgaa gaaggcggct
     2821 cggcgcgcta ccagcccgtg tccgaagatc tcgatagatt ggtggcgggc gccggcgaga
     2881 tgtacgccag gagcccggac gcggtgcggc ggatgatcgt ggcctcggcc catggcggcc
     2941 tcgccgcctt cgccgacgcc ttcaggctgc gaaaggattg aaccgtggcc cagctgttcc
     3001 ccgccgccgt ctacctgctc tgcttcgcga ccagcgcggc ctgcgccttc ctgctcgccc
     3061 gcaattatgc gcgcacgcgg gcgcggctgc tgctgtggag cgctttgtgc ttcgtcctgc
     3121 tcgccgccaa caatctcgtg gtggtgatcg acctgttgct gctgccccag atggacttcc
     3181 gcctggtccg gctgctgctg tcgctgtcgg gggtaagcgt gctgctgttc ggcttcatct
     3241 gggatctaga ggaggattga ggccgtgacg atctacgact tcctctcggg cgcggtgacg
     3301 atgggcttcg tgctggcggg cctgttcttc ctccgcttct ggcggcgcac ccatgacggg
     3361 ctgttcctcg cctttgcgct cgctttctgg ctgctggggc tcacccaggc gttgctgagc
     3421 ttctccaaca tcccggtcga ggagcgcagc tgggtctatt tgttccggct ggcggcgttc
     3481 gcgctgatcc tcgcctcgat cggcctcaag aatttccgcg cccgctccgg ccagcgctag
     3541 cggcatcgtc cagcctcggc gtttcgcccg gccccaatca actgttatgc ttggccgagc
     3601 gaagaaaggc aggattcgac ggtggagccg ctcgatcaga atgggcgggc aatcacggac
     3661 aagtccgacc gcgaccgcat gctcgccgaa ctctatgacg agcttcgcgc cgccgcctcg
     3721 cgcctgctcc gccgcgaggc gccgttcctg acgctcgagc cgaccgatct cgtcaacgag
     3781 gccgcgctgc gcgtcatgaa gctcgacagc atgagctggc acgaccgcca gcatttcttc
     3841 gcgaccgggg cccgcatcct gcgccaggcg atgatcgacg cgatccgccg ccgccgcagc
     3901 gccaagcggc ggatgccgac cttgatgttc gccgacgagg aaggcagcga catcgacgtc
     3961 gaggcgctcg acgaggcgct gacccgcctg gaagtcgcct cgcccgagct ggcccggatc
     4021 gtcgagctgc gcttcttcgt cggcctcggc gtcacggaga tcgcggccgt caccgacagc
     4081 tcggaaagca cggtgaagcg gcgctggcgc accgcccgct tgtggctcgc cgccgaactg
     4141 cagccggatg cgcctcaggc gcccgatcga taagtggaag cgggcgcctc gccgcggcag
     4201 atcgccaaat tgcccctcaa ccccgccacc acattgctct tgtcccactc gttgagcgtg
     4261 cccgcttgtt ccagccgggc gaacgccgcc tcggagctgc gccagatcgc gcaggcgcgg
     4321 cccgcccgcc cggcggtcca gtggagattg ccaagcacct tgcgggaaaa ggccaggtcg
     4381 cgggcggtgc gggtatcggc aggcgcggcc gccagcctcc gttcctcgct ttccacgatg
     4441 ccggcctgga tcgcgaccgc ttcgccatgg cggccggcgt cgctcagcaa ttgcgccgcc
     4501 aacgcctccg tgttgcggat tcgccaggca atcatgcggt cgttcggctc gatcgcccgc
     4561 agcctccgcg cgaccgccag cacgtccagc gccatttcga gaatgccgtc ctcaagcccg
     4621 agcggctcca gcgaggtgat gagattgtag ccgctcgcca gccgcgccga cagcagctcc
     4681 gggcggccag gatggcgcgc cagcgcctcg cgcaccagcc ggtcggcctc gcgcgcgtcg
     4741 agcagcgcct cccgcctgcg gtcgcggtac cagtcgccct cggcccgcgc gttgtgcgct
     4801 tcggcccggg cgatgatata ggccggcagc cgccgctcgg ccgcgggcaa gcgctccagc
     4861 cagccgatcg tgtcggacgc gaacgcgctt tcctcggcat agcgctcgct ccagccgagc
     4921 acgtcgaggc gcaccgtgcg ccgctgattc tccagcgccc gccattccgc gctgccgtcg
     4981 cggccggcgg cgaccagcgg ctcggcggcg cccaatagcc gctcccccgt tgcggtcgca
     5041 ttgtcctgcc agatcgccat gtcggcctgc atcagccgcg cccgggcgag ctcccgcagc
     5101 gccgccgggt tcgccgggcg cacggatgcc agcgggacga gcatcgccgc cgccttgccg
     5161 aggttgcgct tggcggcctc gatccggccg aggttcgggc tgttgggcac accctgcact
     5221 tcggccagcc ggttatagcc catcgcggtc tcgagcttca ccgccgcgga cgcgtccggc
     5281 agcgccgcca ggcggtcgag gtagagctga gcctcgcggg tgagccgctc gcgcgccgga
     5341 agcgtgccgg ggagcgcggc cagctggccg tagagcgtga acagctggaa cttggcgagg
     5401 gagcgcacct cctcgaaccg cgcctcggcc gcggcgcgct ggcggcgggc gtcgaaatag
     5461 aggctggtgg agacgatcgc cgccgccgcg agcaaaatga acaccgcccc gccgatcgcc
     5521 agcggcaggc ggttgcgggc gaggaagcgg cgactgcgat aggccaaagt cggccgcatc
     5581 gcactgatcg gccgcttgtc gcagaaggag ccgaggtcgg cggccagtga ggccacgtcg
     5641 gggtagcggc gtgccggatc ctcggccaac gctttggcga cgatcgcgtc gagctcgccc
     5701 gccagttggc gcgggccgaa ccgccggggc gcttccgccg cgacttcgct catcgcccgt
     5761 ccgtccgggc ggtgcccgct cagcatttcg tggagcaggg cgccgaggct gtagacgtcg
     5821 ctggcgacgg tcggcggctg ccccgccagc cgctcggggg cggcatagcc cttggtcagc
     5881 ggataggggc cttcgatctc gtcctcgtcg agctcggcgg tgaggcgggc gatgccgaaa
     5941 tcgaccagct tgatcgatcc atcctcggcg gcgatgacgt tggacggctt gatgtcggcg
     6001 tggacgacca ggttgcggtg ggcgtggctg accgcctcgc agacagcggc gaaccggtcc
     6061 agcgtctgcg caaggccgag ccgccgccgc tcggtgtcga cggtgaccgg ccttccctcg
     6121 acatattcca tcaccagata gggccgcccg tcggccgagc cgccgtccag gatgcgggcg
     6181 atgccggggt ggctgaggcg ggcgaggatg cgccgctcga gcgcgaaccg ctcctgcgcg
     6241 cgggcgtcct tgaggccggc gcggatcagc ttgatcgcca ccttctgccg gtagacgccg
     6301 tcgtcgcgct cggccgccac caccgtgccc atgccgccgc tgccgatcgt gccggt
//
LOCUS       DASVRK010000286         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_304828, whole genome shotgun sequence.
ACCESSION   DASVRK010000286 DASVRK010000000
VERSION     DASVRK010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304828"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1222)
                     /locus_tag="VF589_11835"
     CDS             complement(227..1222)
                     /locus_tag="VF589_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX8258313.1"
                     /translation="MPDPKLDVLAIGNAIVDVIAAADDAFLETEGLAKGSMRLIDADE
                     ATRLYGRMAAGRETSGGSAANTVAGIAALGLRAGFIGQVAGDQLGEVFSHDIRALGVD
                     FRTAPGNGEVPTARCLILVTPDAQRTMNTFLGASQHLPAAALDEAQIRDAAILYLEGY
                     LWDPEEPRAAMERAIAIAREAGRKVAFTLSDSFCIARHRDGFNALIDSGRIDILFANE
                     DEIKGLSGEEDVEAAVAATAARVPVLVVTRSERGALAVAGGERAEVAAEPVGRVVDTT
                     GAGDLFAAGFLAGQAQGRSLEQSLRIGAIAAAEVISHYGARPEADLKALVAEKLG"
     gene            1330..1704
                     /locus_tag="VF589_11840"
     CDS             1330..1704
                     /locus_tag="VF589_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258314.1"
                     /translation="MTRSRPLSPAAVATLLLAGCAPTVAVQRPATMVQPAAPSFATGG
                     LESVMGQNARSLEARFGRPDLDVREGTARKLQFSGPACVLDAYLYPPGRGGEPVVRYI
                     DARRPDGTDLDRASCVAALNQR"
BASE COUNT          254 a          639 c          591 g          261 t
ORIGIN      
        1 cgcggccccc cgggccgcac tcctatcccg cccttcggct cccaaagcgt tgaagaatcg
       61 aatggaatca ccatcatccg ttcgccctga gcctgtcgaa gcctgtcctg agcgacgccg
      121 caggcggcgt cgaagggggc ctttcttctt gttctggggc gaaaaaagaa agggcttcga
      181 caggctcagc ccgaacggca acagggacaa cttacccttc aacgctctat cccagcttct
      241 ccgccaccag cgccttgagg tccgcctcgg gccgcgcgcc gtaatgggag atcacctccg
      301 ccgccgcgat ggcgccgatg cgcaagctct gctcgagcga tcggccctgc gcctggcccg
      361 ccaggaagcc ggcggcgaac aaatcgccgg cgccggtcgt gtcgaccacg cgtccgaccg
      421 gctccgcggc cacctccgcc cgctccccgc cggccaccgc cagcgcgccc cgctcgctcc
      481 gggtcacgac cagcaccggc acccgggcgg cggtcgcggc gacggccgct tccacatcct
      541 cttcgccgct caaacctttg atctcgtcct cattggcgaa caatatatcg atccggccgc
      601 tgtcgatcag cgcgttgaag ccgtcgcggt ggcgcgcgat gcagaagctg tcggacaggg
      661 tgaaggcgac cttccggccg gcctcgcggg cgatggcgat cgcccgctcc atcgcggcgc
      721 gcggctcctc ggggtcccac aaatagcctt cgaggtagag gatcgcggcg tcccgtatct
      781 gcgcttcgtc gagcgcggcg gcggggaggt gctgggaggc gccgaggaag gtgttcatgg
      841 tccgctgcgc atcgggcgtg accaggatca ggcagcgcgc cgtcggcacc tcgccattgc
      901 cgggcgcggt gcggaaatcg acgcccagcg cgcggatatc gtggctgaac acctcgccga
      961 gctggtcgcc agcgacctgg ccgatgaagc cggcgcgaag cccgagcgcg gcgatccccg
     1021 cgacggtgtt ggcggccgaa ccgccgctgg tctcgcgccc cgccgccatc cggccgtaga
     1081 gccgcgtcgc ctcgtcggcg tcgatcagcc gcatcgaccc cttggcgagc ccttcggtct
     1141 ccaggaaggc gtcgtcggcg gcggcgatca cgtcgacgat ggcgttgccg atggcgagga
     1201 cgtcgagctt cgggtcgggc acggggcagt tactcttgtg aggggagggg aatgcaccgc
     1261 ctagccgcgc cgccagggcg gatcaaccaa ggtgattgac cggcgtcggt ggctgggcga
     1321 agcaggcgca tgacccgctc ccgccctctc tcccccgccg ccgtcgcgac cttgttgctc
     1381 gccggctgcg cgccgaccgt ggcggtgcag cggccggcaa caatggtcca gccggccgcg
     1441 ccaagcttcg ccacgggcgg gctggagtcg gtgatgggcc agaatgctcg ctcgctcgag
     1501 gcccgcttcg gccggcccga cctcgacgtg cgcgagggca cggcgcgcaa gcttcaattt
     1561 tccggcccgg cctgcgtcct cgatgcctat ctttatccgc ccgggcgcgg cggcgagccg
     1621 gtggtccgct atatcgacgc ccgccgcccc gacggcaccg acctcgaccg cgccagctgc
     1681 gtcgccgcgt tgaaccagcg ctaagaccgg cgagcgcgcc agagcgttga agggtcgaac
     1741 ggaat
//
LOCUS       DASVRK010000287         5974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_121344, whole genome shotgun sequence.
ACCESSION   DASVRK010000287 DASVRK010000000
VERSION     DASVRK010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121344"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VF589_11845"
     CDS             <1..951
                     /locus_tag="VF589_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258315.1"
                     /translation="LLNGGADGDDLLGGDGIDTVSYAGSSGRITVRLDVTELQFTFSS
                     GNDRLREIENLIGSDFADRLIGDDDDNRLAGVVGDDELEGGGGNDTLVGGLGADFMTG
                     GTGDDFYIVSDRGDVVIEAAGGGRDTVMASIGYRLGDTIEDLRLAGTAVSGSGNGGDN
                     AILGNRADNRLNGLDGVDELNGGAGDDALSGNAGDDVLIGGAGRDTLTGGAGADLYRF
                     DDGDSAAAAGQSDVIAGFSRGAGDRIDLSAIDADAATAADDGFAFIGGAAFSGAAGEL
                     RATVANGISFVQGDLDGDGSADFTVRVDAPLTLAATDFIL"
     gene            1156..3306
                     /locus_tag="VF589_11850"
     CDS             1156..3306
                     /locus_tag="VF589_11850"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase"
                     /protein_id="HEX8258316.1"
                     /translation="MIGSGEAARRGAVAELWWDSRADMWGAAAEATPRVAEQEGNDSA
                     ETAQRLDREAFAVAGNAALRDDTLTSLAVEAGLSSAGDLDVFSVWLEAGETLTLDVDS
                     GGDGLGAAPEMIAFTRGPAGEFLGGGGYGEALVTVYARTAGYYSFELQTESGAVTDAY
                     LLHLSLERTGVDPDAAAVLNGRQWEGLDLRYSFPVTAAEHSSGEFENYEALSAVQRAV
                     VRGGLSAISGFTDLSFAEAAPGERRSAHLRYGMTDSTPVASAIGPGLGADSGGSIYHN
                     SSGRFDNPVLGSHAFATFLHETGHALGLKHGHQPPAMSAEHDSTEFSIMTYRAYVGAT
                     PGLRQEEYGYAQSFMMYDIAALQEVYGADFETRAGATSYRWSATTGRMFVDDVGQMIP
                     GANRVFLTVWDGGGRDTYDLSNYRNSVAIDLRPGEWTITSEAQLAKIGAYDGEIFARG
                     NVANALLYRGDTRSLIENAVGGTKGDTIVGNQAANRLDGGAGDDRIDGGEGRDVLIGG
                     AGRDVFLVDHALDRAVEASGGGYDEVRSAAASFTLAGGVEALVLTGAATAGHGNGLAN
                     RITGNDGANLLSGGLGGDVIAGGEGADRIEGDGGADRLRGGEGADRFVFDAALNRNTN
                     VDALLDFSVADDTILLDRAVFGGIDRDGALRLSAFWAGTAALDETDRIVYDAATGRIF
                     YDADGSGAAAAVLFAEVRPGLALTHQDFAAYSGG"
     gene            complement(3803..>5974)
                     /locus_tag="VF589_11855"
     CDS             complement(3803..>5974)
                     /locus_tag="VF589_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258317.1"
                     /translation="SLSSTPIGDRISFYEPNAREGVFADLRTGIVYNDGFGNRETMTG
                     IESLSGSNVHIDRLYGNDGANFLALGTGDTLKAFGGDDILNLTGAAELIDGGDGIDTL
                     QGISGTKLVDEDGDGNAETVTNEGGVTIDLAAGTILDAWGDTGTIAGVEHFGGSMGSG
                     DDLLTGDGNDNVIRGFGGVDRIDGGAGSDTISFSAWGREEHYVYHGRSWGAVEVDLAA
                     GYSEETALKSGTVRETTERFGRDTLIGIENVIGTGLGDRLLGDDSDNIIAPGAGNDFV
                     DGRGGSDTVDYGAARAAVAIDLGAKTAEEAGSGAPSRQVSWTDADGNSQTALVEADGA
                     VASRDRLLNIENAIGSAHADRLVGSAAANWLDGGRGNDALGWSGGGDTLVGGAGSDTA
                     DYSAAAAAALIDLAAGAGSVSGQAGTDTLIAIENASGTAFADRLVGNRAANRLDGGAG
                     IDRMEGGAGNDIYVVDDRRDSILDTSGVDRIDASVSLTLADGLEQLLLTGEGDLSATG
                     NAAANRLTGNAGSNRLDGRGGADAMAGGHGNDVYVVDAAGDRVTEAPSAGTDRVEASI
                     GVTLARHVENLTLTGTGDIDGTGNADTNIILGNGGDNVLAGGGGTDRLTGGAGRDAFL
                     FDTTLRISGGDLILDFASADDTIRLDRSVYTGFAETGTLRLAAFREGTRAQDDSDRIL
                     YDQATGKIFYDADGSGDGAAILFAKLDAGTALTVADFVIVA"
BASE COUNT          871 a         1876 c         2295 g          932 t
ORIGIN      
        1 ctgctgaacg gaggggccga cggggacgat ctcctcggcg gggacgggat cgacaccgtt
       61 tcctatgcgg gcagcagcgg ccggatcacg gtccggctcg acgtgacgga gctccaattc
      121 accttctcca gcggcaacga ccggctgagg gagatcgaga atctgatcgg ctccgatttc
      181 gccgaccgtc tgatcggcga tgacgacgac aaccggctgg cgggcgtcgt cggcgacgac
      241 gagctcgagg gcggcggcgg caacgatact ctggtcggcg gcctcggcgc cgacttcatg
      301 accggcggca ccggcgacga tttctacatc gtcagcgacc gcggcgacgt ggtgatcgag
      361 gcggcaggcg ggggccgcga cacggtgatg gcgtcgatcg gctaccggct cggcgacacg
      421 atcgaagacc tccggctggc gggcacggcg gtgagcggca gcggcaatgg cggcgacaat
      481 gcgatcctcg gcaaccgcgc cgacaatcgc ctcaacgggc tcgacggcgt ggacgaactc
      541 aacggcgggg cgggcgatga cgcgctgagc ggcaatgccg gcgacgatgt cctgatcggc
      601 ggcgccgggc gcgacacgct gaccggcggc gcgggcgccg acctctatcg cttcgacgat
      661 ggcgacagcg cggccgcggc ggggcagtcc gacgtgatcg ccggcttctc gcgcggggcc
      721 ggagaccgga tcgacctgtc ggcgatcgac gccgacgcgg ccaccgcggc ggacgacggc
      781 ttcgccttta tcggcggcgc cgccttttcg ggcgcggcgg gtgagctgcg cgcgacggtc
      841 gccaacggca tcagcttcgt gcagggcgac ctggacggcg acggcagcgc cgacttcacc
      901 gtccgcgtcg acgcgccgct tacgctcgcg gccaccgatt tcatcctcta gagcggattt
      961 gggctgagtt gcgccccagc gaaggctggg gcctacggga ttctcggagc tgggcccccg
     1021 ccttcgcggg gggactgggc ccccgccttc gcgggggaac gctggccgag gccttcgcgg
     1081 gggaacgttt tgtcgcttgt cgggaagcgt ttagcaggcc tagtctggtc accgggcaac
     1141 agtcgggggg cggccgtgat cggatcgggg gaagcagcgc ggcgcggggc ggttgcggag
     1201 ctttggtggg atagccgggc ggacatgtgg ggcgcggcgg cggaggcgac gccgcgggtg
     1261 gccgagcagg agggcaatga cagcgccgag acggcgcagc ggctggaccg ggaggcgttc
     1321 gccgtggccg gcaatgcggc gctccgcgac gacacgctga cctcgctcgc ggtcgaggcg
     1381 gggctttcga gcgcgggcga tctcgatgtg ttcagcgtct ggctggaggc cggcgagacg
     1441 ctgacgctcg acgtggattc aggcggcgac gggcttggcg ccgctcccga gatgatcgcc
     1501 ttcacgcgcg ggcccgcggg cgagttcctc ggcggcggcg gctatggcga ggcgctggtc
     1561 accgtctatg cgcgcacggc cggctattat tcgttcgagc tgcagaccga atcgggcgcg
     1621 gtgaccgacg cctatctgct gcacctcagc ctcgagcgca ccggcgtcga tccggacgcc
     1681 gcggcggtgc tgaacgggcg gcaatgggag gggctcgacc ttcgctacag cttcccggtc
     1741 accgctgccg agcatagcag cggcgaattc gagaattacg aggcgctcag cgccgtgcag
     1801 cgggcggttg tgcgaggcgg gctgagcgcg atctcggggt tcaccgactt gagcttcgcc
     1861 gaggccgcgc cgggcgagcg gcggtccgcg cacctgcgct acggcatgac cgactcgacg
     1921 ccggtggcga gcgcgatcgg gccggggctc ggcgccgatt cgggcggatc gatctaccat
     1981 aacagcagcg gcaggttcga taatccggtg ctcggcagcc acgcctttgc gaccttcctg
     2041 cacgagaccg gccatgcgct ggggctgaag cacggccacc agccgccggc gatgagcgcg
     2101 gagcatgatt cgaccgaatt ttcgatcatg acttaccgcg cctatgtcgg cgcgacgccg
     2161 gggctgcggc aggaggaata tggctacgcg cagagcttca tgatgtacga catcgcggcg
     2221 ttgcaggagg tttacggcgc cgatttcgag acgcgggcgg gggcgaccag ctatcgctgg
     2281 agcgcgacga ccgggcggat gttcgtggac gacgtggggc agatgatccc gggcgcgaac
     2341 cgtgtgttcc tgaccgtctg ggacggcggc gggcgcgaca cgtacgatct gtccaattac
     2401 cgcaattcag tcgcaatcga cctgcggccg ggcgagtgga cgatcacgtc ggaggcgcag
     2461 ctggcgaaga tcggagccta tgacggggag attttcgcgc gcggcaatgt cgccaacgct
     2521 ttgctctatc gcggcgacac gcgatcgctg atcgagaatg cggtcggcgg gacgaagggc
     2581 gacacgatcg tcggcaacca ggcggcgaac cggctggacg gcggggcggg cgacgaccgg
     2641 atcgacggcg gcgagggtcg cgacgtgctg atcgggggcg cgggcaggga tgtgttcctg
     2701 gtcgaccatg cgctcgaccg ggcggtcgag gcgagcggcg gcggctatga cgaggtgagg
     2761 agcgcggcgg cgagcttcac cttggcgggc ggggtcgagg ctttggtgct gaccggggcg
     2821 gcgacggcgg gccatggcaa cgggctcgcc aaccgcatta cggggaatga cggggcgaac
     2881 ttgctcagcg gcgggcttgg cggcgacgtc attgcgggcg gcgaaggggc ggatcggatc
     2941 gagggggacg gcggggcgga ccggctgcgc gggggcgagg gcgcggatcg gttcgtgttc
     3001 gacgcggcgc tgaaccgcaa cacgaacgtc gacgctttgc tcgatttctc ggtggcggac
     3061 gacacgatcc tgctcgaccg ggcggtgttc ggcgggatcg accgcgacgg agcgctgagg
     3121 ctgtcggcgt tttgggcggg gaccgcggcg ctcgatgaga ccgaccgcat cgtctacgac
     3181 gcggcgaccg ggcgtatctt ctacgacgcc gacggatcgg gcgcggcggc ggcggtgctg
     3241 ttcgccgaag tgcggcccgg gctggcgctc acccaccagg atttcgccgc ctatagcggc
     3301 ggctgacggc gaagttcccg cgcgattttg ccgaacgcac cgcttaaaaa tggcggtatt
     3361 gagcggggga ggctcagcgt catcccggat gaccaccgtc atccccccaa gtcctcccct
     3421 gccttgcatg atgggaccat ggtggagggg cctcttagct cctccccggg atttcccggg
     3481 gagggggacc gcacaggacg gtcgaggggc gcgagcgaag cgagctcttt gagcggaaga
     3541 agggcgcttc gcgccgcccc tccaccatgc tgcgcatggt ccccctcccc tgcaaaagca
     3601 ggggaggaat tagagatccc agctttcgct gggatgacgg tggggatccg gttccccccg
     3661 ctcaataccc actaagtcca aattgctgag ctccgtcgcc ggatcgacgc gggcaaacag
     3721 caatgccgcc gccccgccgg cgccgtccgc gtcgaagaag atgcggacgg ttggcttgtg
     3781 gtagatcagc cggtcctccc gatcaggcga cgatgacgaa gtccgcgacg gtcagcgcgg
     3841 tgccggcgtc gagcttggcg aacaggatgg cggcgccgtc gccgctgccg tcggcatcgt
     3901 agaagatctt gccggtggcc tggtcgtaga ggatgcggtc gctgtcgtcc tgcgcccggg
     3961 tgccttcgcg gaaggcggcg aggcggagcg tgccggtctc cgcgaagccg gtgtagacgg
     4021 agcggtcgag ccggatggtg tcgtcggcgg aggcgaagtc gaggatcaga tcgccgccgg
     4081 agatgcgcag cgtcgtgtca aacaggaagg cgtcgcggcc ggcgcctccg gtcagacggt
     4141 cggtgccgcc gccgccggcc agcacattgt cgccgccatt gccgaggatg atgttggtat
     4201 cggcgttgcc ggtgccgtcg atatcgccgg tgccggtgag ggtgaggttc tcgacgtggc
     4261 gggcgagggt gacgccgatg ctcgcctcga cccggtcggt tccggccgag ggggcttcgg
     4321 tgacgcggtc gccggcggcg tcgacgacat agacgtcgtt gccatggcct ccggccatcg
     4381 catcggcgcc gccgcggccg tcgagccggt tcgagccggc attgccggtg aggcggttgg
     4441 cggcggcgtt gccggtggcg gagagatcgc cttcgccggt gagcaggagc tgctcgaggc
     4501 cgtcggcgag ggtgaggctg acgctggcgt cgatgcggtc gacgccggac gtgtcgagga
     4561 tcgaatcgcg gcggtcgtcg acgacataga tatcgttgcc ggcgccgcct tccatgcggt
     4621 cgatgccggc gccgccgtcg agccggttgg cggcgcgatt gccgacgagg cggtcggcga
     4681 aggcggtgcc ggaggcgttc tcgatggcga tgagcgtgtc ggtgccggcc tggccggaaa
     4741 ccgaaccggc gccggcggcg agatcgatca gggcggcggc ggcggcggcc gaataatcgg
     4801 cggtgtcgct gccggcgccg ccgaccagcg tgtcgccgcc gccgctccag ccgagcgcgt
     4861 cgttgccgcg gccgccgtcg agccagttgg cggcggcgct gccgacgagg cggtcggcat
     4921 gggcggagcc gatcgcattt tcgatgttga gcagccggtc gcgcgaggcg acggcgccgt
     4981 ccgcttcgac aagggccgtc tgcgaatttc cgtccgcgtc cgtccatgag acctgccgcg
     5041 atggagcgcc gctgccagcc tcctcggcag tcttggcgcc gaggtcgatc gcgaccgccg
     5101 cgcgggcggc gccatagtcg acggtgtcgc tgccgccgcg accgtcgacg aagtcgttgc
     5161 cggcgccggg tgcgatgatg ttgtcggaat cgtcgccgag caggcggtcg ccgaggccgg
     5221 tgccgatgac gttctcgatg ccgatcagcg tgtcgcgccc gaaccgctcg gtcgtctccc
     5281 tcacggtgcc ggacttgagg gcggtctcct cggaatagcc ggcggcgagg tcgacctcga
     5341 ccgcgcccca tgagcggccg tggtagacat aatgttcctc gcgtccccag gccgagaagc
     5401 tgatcgtgtc gctgcccgcg ccgccgtcga tccggtcgac gccgccgaag ccgcggatga
     5461 cgttgtcgtt gccgtcgccg gtcaggagat cgtcgccgct gcccatcgag ccgccgaaat
     5521 gttcgacgcc ggcgatggtg ccggtgtcgc cccacgcgtc gaggatggtg ccggcggcga
     5581 ggtcgatggt cacgccgccc tcgttggtga cggtttcggc attgccgtcg ccatcctcgt
     5641 cgacgagctt ggtgccggag atgccctgaa gcgtatcgat gccgtcgccg ccgtcgatca
     5701 actcggcggc gccggtgagg ttcaggatat cgtcgccgcc gaacgccttc agcgtgtcgc
     5761 ccgtgcccaa agccaggaag ttggcgccgt cattgccgta gaggcgatcg atatggacat
     5821 tgctgccgct cagcgattcg atgccggtca tcgtttcgcg attaccgaag ccgtcattgt
     5881 agacgatgcc ggtgcgaaga tccgcgaaca cgccctcgcg cgcgttcggc tcgtagaagc
     5941 tgatgcgatc gccgatcggg gtcgatgaga gcga
//
LOCUS       DASVRK010000288         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_121399, whole genome shotgun sequence.
ACCESSION   DASVRK010000288 DASVRK010000000
VERSION     DASVRK010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121399"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /locus_tag="VF589_11860"
     CDS             <1..2204
                     /locus_tag="VF589_11860"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8258318.1"
                     /translation="MEQPVAAMRFFSEAAALGAPASELAKDRGLAYDLAGDQRRAQAD
                     YKLALARGDDQEVRRRLALSLAISGDKDGALRLVDEQMRRRDRAAWRIRAFVLALAGD
                     ADGATNAARQVMPGQAPSMQLFFASLPALSPAQRAMAVHYGHFPNGQAAQLAQVAQSM
                     KAPPPVVQAARPTGKQAGGRSRSASSRRAARSTAAPPTALARNEAVQRPAMETVRRRP
                     GAKPTDVAAYEPVATRPVRRPPPAAATRPVQVASNNVAALPARIAPTARPMPSPITTA
                     PRQPALGPPAYVPTARPVQLPPASVSAAPMAAAPRPASPGFASNPPLRSQAPVPAPTP
                     SAAPSTAPATNAPAAAALQPRGVPFGPPTPAPQQPQAFATASPPATNAPAAAALQPRG
                     IPFGPPTPAPQQPQAFASSASPLAPSGGMAAATAAPGFASLPSNPAPTLAEPVSPPAQ
                     QAPAAPIVAPAPQPSLPAAAQPQPQPQPQPANPDAVTTVALPPATPVPLPSAAPTTSP
                     SETLPRDPAPTPVATAAPSVASSPAPMAFAGRAPFGPPTPAPAAVAPAAPPAAVPFGP
                     PTTGPRPGFADVAATVNSLPDVVQPAKPAAPQPRLAQVEEPKPKPAPAKPAPAAATEA
                     KKAPAKEAKKKPEPAQPKAPSRVWVQVAGGAEKARLPGEFERLKEKAPKLLAGRTAWT
                     TPLRFTNRLLVGPFKTEKEAQEFVNELAKLKLTGFSWTSAAGQEIEKLPAK"
     gene            2201..3364
                     /locus_tag="VF589_11865"
     CDS             2201..3364
                     /locus_tag="VF589_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX8258319.1"
                     /translation="MRPPAPYASDPARSRGRLHPAGGGETRGPRDAFQRDRDRIIHSV
                     AFRRLRQKTQVFVAPDGDHFRVRLTHSLEVAQIGRTLARALGLNEDLTEALCLAHDVG
                     HPPFGHAGEDALKAAMAEAGGFDHNAHTLRLLTRLETPYPRHAGLNLSWETLEGLAKH
                     NGPVPKPGWALGEADAEFPLRLESWPSLEAQVAAIADDVAYNNHDIDDGLRAGLFTLE
                     DLLEVPFVADTWRAVRDRYEDVPEAALVPELIREQIGHMVNDVLGETGRRIAAAGVAD
                     PDDVRAAGRPLAGFSAAMADEERRLKAFLYARMYGAPPVKAVADRAQAVIAGLYAAYR
                     ADPALLPADWQADMGSETETLRRIGDFIAGMTDRYAIARHRELVGPVALPEAS"
     gene            3427..5160
                     /gene="argS"
                     /locus_tag="VF589_11870"
     CDS             3427..5160
                     /gene="argS"
                     /locus_tag="VF589_11870"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX8258320.1"
                     /translation="MTLYARFAAHIERVLDSLEAAGQLPAGIDRLAVAVEPPRDPAHG
                     DLSTNAAMVLAKRAATNPRALAALIAPALEALDEVERAEIAGPGFINIRLRREIWEQE
                     LRAIIEAADGYGRSALGGGRRVNVEYVSANPTGPMHMGHCRGAVVGDALASLLEHAGY
                     AVTREYYVNDAGSQVDTLARSAHMRYLEALGSDIGEIPEGLYPGDYLKPVGEMLAAEY
                     GDRFAAAPEAEWLDPFKRRTVAAMLDLIRADLALLGIHHDKFASEAEVQASGAPDRVL
                     ATLQGKGLVYQGTLEPPKGTEPDDEWEPVELTLFRSTQYGDDQDRPMKKSDGSWTYFG
                     ADAAYHLAKADEADALINIWGADHAGTVKRIKAAVAALTEGKVPFDVKLVQMVRLLRA
                     GEPVKMSKRSGNFVTLADVVQEVGKDVVRFTMLTRKADAQMDFDFAKVVEASKDNPVF
                     YVQYAHARISSLHRKAAEEGIDVSAPADLALLDEEELALVRVAAQFPRVVEAAAQAHE
                     PHRIAFYLNDLAAAFHALWNKGNDDPLRRFLLADKPELTRARLELASAVAQVIRNGLG
                     IMGVAAAEEMH"
     gene            5160..>5358
                     /locus_tag="VF589_11875"
     CDS             5160..>5358
                     /locus_tag="VF589_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258321.1"
                     /translation="MAGSRLGGAGDRDSRPWLEPVEVEQEPSGPRLGKPILFAILALV
                     ILGAAVGAYSWWSSQGATEGHG"
BASE COUNT          791 a         2007 c         1846 g          714 t
ORIGIN      
        1 agatggagca gccggtcgcc gcgatgcgct tcttcagcga ggcggccgcg ctcggcgcgc
       61 cggcgtccga actggccaag gatcgcggcc tcgcttacga ccttgccggc gaccagcgcc
      121 gcgcccaggc cgattacaag ctggcgctgg cccgcggcga cgaccaggag gtgcggcggc
      181 ggctcgcgct gtcgctggcg atcagcggcg acaaggacgg cgcactgcgg ctggtcgacg
      241 agcagatgcg gcgccgcgac cgcgcggcgt ggcggatccg cgcgttcgtg ctggcgcttg
      301 ccggcgatgc cgacggcgcc accaacgccg cgcgccaggt gatgcccggc caggcgccct
      361 cgatgcagct tttcttcgcc agccttccgg cgctgagccc cgcccagcgc gcgatggcgg
      421 tccattacgg tcacttcccc aacggccagg cggcgcagct ggcgcaggtg gcgcagagca
      481 tgaaggcgcc gcccccggta gttcaggccg ccaggccgac cggcaaacag gccggtggcc
      541 gctcccgcag cgcgtcgtcg cggcgggcgg cgaggagcac ggcggcgccg ccgaccgcgc
      601 tggctcggaa tgaagcggtg cagcggcctg ccatggagac ggtccggcgc cgcccgggcg
      661 ccaagccgac cgatgtcgcc gcctatgaac cggtcgcgac gcggccggtc cggcgaccac
      721 ccccggcggc ggcgacgcgg ccggtgcagg tggccagcaa caacgtcgcg gcgttgccgg
      781 cgcggatcgc ccctactgcc cgcccgatgc cttcgccgat tacgaccgcg ccgcgccaac
      841 cggcgctcgg gccgccggcg tacgtaccga cggctcgccc ggttcagctg cctccggcct
      901 ccgtcagcgc tgcgccaatg gctgcggctc cccggccggc aagtcctggg ttcgcctcca
      961 atccgccgct ccgcagccag gctccggtcc cggcgcccac tccgtccgca gctccgagca
     1021 cggcgcctgc gacgaacgca ccggcagccg ctgcgctgca gccgcgcggc gttccgttcg
     1081 ggccgccgac tccggctccg cagcaaccgc aggcctttgc caccgcctcg ccgcccgcca
     1141 ccaacgcgcc ggccgccgcc gccctgcagc ctcgcggcat cccgttcggg ccgccgacgc
     1201 ctgctccgca gcaaccgcag gcgtttgcca gcagcgcatc gccgttggcg ccgagtggag
     1261 ggatggctgc ggcaacggcg gctccgggct tcgcgtcgct gccgtccaat cccgcgccca
     1321 cgctggccga gccggtttcg ccgccggccc agcaagcgcc cgccgcgccc atcgttgcgc
     1381 cggcgccgca gccttcgctg ccggccgctg cccagcccca gccccagccc cagccccagc
     1441 cagcgaaccc ggacgcggtg accacggtcg ccttgccgcc cgctacccca gtgccgttgc
     1501 ccagcgcggc accgaccacc tcgccctcgg agacgctgcc gcgtgacccc gcgccgactc
     1561 ccgtggcaac ggcggcaccg tcggttgcct cgagtcccgc tccgatggcg ttcgcgggcc
     1621 gggcgccgtt cgggcctccc acgccggcac cggcggctgt cgcgccggcc gcgccgcccg
     1681 cggctgtgcc gttcgggcct ccgacgacag ggccaaggcc cggctttgcc gacgtcgccg
     1741 ccacggtgaa ttcgcttccg gacgttgtgc agcccgccaa gccggccgcg ccgcaacccc
     1801 gcctcgccca agtcgaggag cccaagccca agcccgcgcc cgccaagccg gctccggcgg
     1861 cggcgaccga agccaagaag gcgccggcca aagaggccaa gaagaagccg gagccggcgc
     1921 agcccaaggc gccgagccgc gtctgggttc aggtcgccgg cggagccgag aaagccaggc
     1981 ttcccggtga gttcgagcgg ctgaaggaaa aggcgcccaa gctgctcgcc ggccggaccg
     2041 cctggacgac gccgctgcgc ttcactaacc gcctcctggt cggcccgttc aagaccgaga
     2101 aggaagcgca ggaattcgtc aacgagctcg ccaagctgaa actgaccggc ttcagctgga
     2161 ccagcgcggc cggccaggag atcgagaagc tgcccgccaa gtgaggccgc cggcgcctta
     2221 tgcgtcggac ccagcacgat cgcgcggccg gctccacccg gccggcggcg gcgagacccg
     2281 cgggccccgc gacgccttcc agcgcgaccg cgaccggatc atccattcgg tcgcgttccg
     2341 gcgcctgcgc cagaagacgc aggtgttcgt cgcgcccgac ggggaccatt tccgggtccg
     2401 cctcacccac agcctcgaag tggcgcagat cgggcgcacg ctggcccggg cgctcggcct
     2461 caacgaggat ctgaccgagg cgctgtgcct cgcccacgat gtggggcatc cgccgttcgg
     2521 ccatgccggg gaggacgcgc tgaaggcggc catggcggag gccggcggct tcgaccacaa
     2581 tgcccacacg ctgcggctgc tgacccggct cgaaacgcct tatccgcgcc atgccggcct
     2641 caacctcagc tgggagacgc tggaagggct cgccaagcat aacggccccg taccgaagcc
     2701 cggatgggcg ctaggcgagg ccgacgccga attcccgctg cggctcgaaa gctggccgag
     2761 cctggaggcg caggtggcgg cgatcgcgga cgacgtcgcc tataacaatc acgatatcga
     2821 cgacggcctg cgcgccggcc tgttcacgct cgaagacctg cttgaagtcc cgttcgtcgc
     2881 cgacacctgg cgggccgtgc gcgaccgcta cgaggacgtg ccggaggccg cgctcgtgcc
     2941 cgagctgatc cgcgagcaga tcgggcatat ggtcaacgat gtgctgggcg agaccggccg
     3001 gcgcatcgcc gctgccggcg tcgccgaccc ggacgatgtc agggccgcgg gccgcccgct
     3061 ggcaggcttc tcggccgcga tggcggacga ggaacgccgc ctcaaggctt tcctctacgc
     3121 ccgcatgtat ggcgctccgc cggtgaaggc ggtcgccgac cgggcccagg cggtgatcgc
     3181 cggcctctac gccgcctacc gcgccgaccc ggctttgctg ccggccgact ggcaggccga
     3241 tatgggtagc gagaccgaga cgctgcggcg gatcggcgac ttcatcgccg gcatgaccga
     3301 ccgctatgcg atcgcgcgcc accgcgagct ggtcgggccg gttgccctcc cggaggcctc
     3361 ctagaaagct ggaccttgtc ggcggcgatc cgctaaggcc cccgctttcc acgaatggac
     3421 ctgaacgtga ccctttacgc ccgctttgcc gcgcatatcg agcgggtgct cgacagcctt
     3481 gaggctgccg gacagctgcc tgcggggatc gatcggctcg cggtcgccgt cgagccgccg
     3541 cgcgatcccg cccatggcga cctttccacc aacgccgcga tggtgctcgc caagcgcgcc
     3601 gccaccaacc cgcgcgcgct ggcggcgctg atcgctcccg cgctcgaagc gctggacgaa
     3661 gtcgagcggg cggagattgc cgggcccggc ttcatcaata tccgcctcag gcgcgagata
     3721 tgggagcagg agcttcgcgc catcatcgag gcggcggacg gctacggccg ctccgcgctc
     3781 ggcggcgggc ggcgggtcaa tgtcgaatat gtctcggcca accccaccgg cccgatgcac
     3841 atgggccatt gccggggcgc cgtcgtcggc gacgcgctcg ccagcctgct cgaacatgcc
     3901 ggctatgccg tcacccgcga atattatgtc aacgacgccg gcagccaggt cgatacgctc
     3961 gcccgttccg cccacatgcg ctatctcgaa gcgctcggca gcgacattgg cgagataccg
     4021 gaggggctct atcccggcga ctatctgaag ccggtcggcg agatgctcgc cgccgaatat
     4081 ggcgaccgct tcgccgccgc gcccgaggcc gaatggctgg acccgttcaa gcgccgcacc
     4141 gtcgcggcca tgctcgacct gatccgcgcc gatctcgcct tgctcggcat ccaccacgac
     4201 aaattcgcgt ccgaggccga ggtccaggcg tccggagcgc ccgaccgcgt gctcgcgacg
     4261 ctccagggca aggggctcgt ctaccagggc acgctcgagc cgcccaaggg gaccgagccg
     4321 gacgacgagt gggagccggt cgagctcacc ttgttccggt cgacccaata tggcgacgac
     4381 caggaccggc cgatgaagaa gtcggacggc agctggacct atttcggcgc cgacgccgcc
     4441 tatcacctcg ccaaggcgga tgaggccgat gcgctgatca acatctgggg cgccgaccat
     4501 gcgggaaccg tgaagcggat caaggcagcg gtcgcggcgc tgacggaagg gaaggtgccg
     4561 ttcgacgtca agctcgtcca gatggtgcgg ctgctgcgcg ccggcgagcc ggtgaagatg
     4621 tcgaagcggt ccggcaactt cgtgaccctg gccgatgtcg tgcaggaagt cggcaaggat
     4681 gtcgtccgct tcaccatgct aacccgcaag gcggacgcgc agatggactt cgacttcgcc
     4741 aaggtggtgg aagcgtccaa ggacaatccg gtcttctacg tccaatatgc ccacgcccgg
     4801 atctcctcgc tccaccgaaa ggcggccgag gaggggatcg acgtctccgc gcccgccgat
     4861 ctggcgctgc tcgacgagga ggagctggcg ctggtccgcg tcgcggcgca gttcccccgc
     4921 gtggtcgagg cggcggcgca ggcgcatgag ccgcaccgga tcgcattcta cctgaacgat
     4981 ctcgccgccg ccttccacgc cttgtggaac aagggcaatg acgatccgct ccggcgcttc
     5041 ctgctcgccg acaagccgga attgacccgc gcccgcttgg agcttgcgtc ggcggtggcg
     5101 caggttatcc gtaacggtct cggcatcatg ggcgtggccg ccgcggagga gatgcactga
     5161 tggcaggttc gcggctcggc ggcgcaggcg acagggacag ccgtccctgg ctcgagcctg
     5221 tggaggtcga gcaggagccc tccggccccc gcctcggcaa gccgattctt ttcgccatcc
     5281 tggctctggt gatcctcggc gcggcggtcg gcgcctacag ctggtggtcc agccagggag
     5341 ccacggaagg gcatggcg
//
LOCUS       DASVRK010000289        23998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_494238, whole genome shotgun sequence.
ACCESSION   DASVRK010000289 DASVRK010000000
VERSION     DASVRK010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 23998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23998
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494238"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..77)
                     /locus_tag="VF589_11880"
     tRNA            complement(2..77)
                     /locus_tag="VF589_11880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Lys,seq:ttt)
     gene            complement(89..580)
                     /gene="folK"
                     /locus_tag="VF589_11885"
     CDS             complement(89..580)
                     /gene="folK"
                     /locus_tag="VF589_11885"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX8258322.1"
                     /translation="MPSSLYAIALGSNRPGRHGSPAAAVRAAAAALGVARLSRIRATP
                     ALGPAGRGFANAAAILDSSLDPPALLALLKRIERGFGRRPGRRWGPRVIDLDIILWSE
                     GAYAGPGLIVPHPEFRRRFFVLDPLAELVPLWRDPLTGLTVRQLRARLARPSPVDRRR
                     ARP"
     gene            695..1351
                     /locus_tag="VF589_11890"
     CDS             695..1351
                     /locus_tag="VF589_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8258323.1"
                     /translation="MYEPISPAPAAEPPRDCPLCPRLVAFREALRAEHPDWWNAPVPN
                     FGDPDAWLVICGLAPGKHGANRTGRPFTGDYAGVLLYETLLKFGLAEGRYEERPDDSL
                     RLKGVAIVNSVKCLPPQNKPTPEESATCRSYFTLALAALGNARVQVALGQIAHQAAVR
                     TAGGRLADYRFGHLAEHRLPDGRILVDSYHCSRYNQNTGRLTAAMFEAVFARALELRP
                     "
     gene            complement(1592..2440)
                     /gene="aguB"
                     /locus_tag="VF589_11895"
     CDS             complement(1592..2440)
                     /gene="aguB"
                     /locus_tag="VF589_11895"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEX8258324.1"
                     /translation="MSEITVAALQLAFSDDIDANIAAVSELVREAAGKGAKLILPPEL
                     FEGHYFCRTQDDAHFTRALSADAHPAVLAMRALAAELRVYIPTSFFEAHGHHHYNSLA
                     MIDDQGAVMGVYRKSHIPDGPGYSEKFYFRPGNTGFKTYKTPFGTVGPAVCWDQWYPE
                     TARALMLDGAEILLFPTAIGAEPHDPTLDTSRMWRRAMIGHAVSNVVPVVAANRIGTE
                     HGQRFYGHSFICDQRGDILAEFGAEETGVLTATLDLAQARAHRAAFGFFRDRRPELYG
                     RLTQDI"
     gene            complement(2632..3609)
                     /locus_tag="VF589_11900"
     CDS             complement(2632..3609)
                     /locus_tag="VF589_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX8258325.1"
                     /translation="MTSRQPPEWAPHEWVWIGFPSHADLWSEDLDAARAEVAAFAELV
                     HAGGAGEQVRLVAADPKAAEAARALAPWAEVVQEPFGDIWLRDTGPIILKDHGMPAAA
                     SFRFNGWGGKYDLEGDDTIGLRLAETADLDARRHDWILEGGAIDVDGTGLAVTTEQCL
                     LNPNRNPGLGRHEVEARLAADLGLERVLWLGEGLLNDHTDGHVDNIARFVGANRLAIP
                     VPAGADDPNAAIYADAAARARAFGVEVVPLPSPGLIERDGEIAPASYMNFYIGNAAVA
                     VPVYGAANDAAAVDAIGALFPGRKALGLRADHVLIGGGSFHCISQQMPV"
     gene            complement(3615..5270)
                     /locus_tag="VF589_11905"
     CDS             complement(3615..5270)
                     /locus_tag="VF589_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8258326.1"
                     /translation="MTAAGAALALAGCTTTGPRPAATAGAAEPALSEETLRTVTERLA
                     SDEFEGRAPTTAGEEKTVRLIAERFERAGLEPGNKGSWYQNVPLAENTASPTALTVAG
                     GKAPLSFAYRTDFVANSYQLQPRIDLKDSEIVFVGYGINAPERGWNDYAGLDVRGKTV
                     IILVNDPDYQSQGLEGPFGGRAMTYYGRWTYKYEEAARQGAAAAFIVHETEPAAYGWN
                     VVQSSWTGPQYNMDAPDNHMDQSKAIGWITREAAQRLFANAGQDYARLSAAARQKGFK
                     AVSLGSQASISLTNQIKRQESKNVIGILRGRARPDEYVIYTAHWDHLGRCDADATGDD
                     ICNGALDNATGTAGLIALAEAHAKAGAPDRSIIFLAVTAEESGLLGSRYYGENPVYPH
                     AQTAGGINMDGLNVAGTTRDLVVIGMGKSELEDYLRRAGGGLEVKNEPTPEAGYYYRS
                     DHFSLAKFGVPMLYAEGGEDLVEGGVAAGRAAAEDYRANRYHQPSDEYDPNWNWSGAI
                     RDLGLYYRIGRELAMSDAWPNWYEGDEFRAIRDKSRAGAQGAQ"
     gene            5431..7431
                     /locus_tag="VF589_11910"
     CDS             5431..7431
                     /locus_tag="VF589_11910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8258327.1"
                     /translation="MLKKLIIPAVAGAAALHSGAAAQQAAPSATMFGAREAVESIDIS
                     PDGTKLVYLAPAAGASSVALVGDLAGGKPAVVATSSGKPDRLSWCRFVSNSRIICRAY
                     ALIDNKGVLIPFGRLFSMDANGGDMKELGQSLSYFDSRIRQFDGSILDWMPEDNNAVL
                     MARDYIPEEGRIGTRLVRQKDGLGVDRIDVRTLKATPVEPVSRTASDFMTDGRGKVRI
                     REIQNRRAGASDQLMAQTISFSYRRTGRSDWEDFSTYNMMNREGTYPVAIDASLNAAY
                     ALRKLNGRFALYRVKLDGSMATELVYANPRVDVDNVVRIGRGGRVIGVTFAEEARSTV
                     YFDPEYAKLAKSLSKAIPNLPLVRFAGSSNDNNKLLIFAGSDSDPGRYYVYDKKARAL
                     NEVMLARPQLENMKLATMKPVTYAAADGVQIPAYLSVPPGKDPKNLPAIVLPHGGPTA
                     RDEWGFDWLTQFLVAQGYAVLQPNYRGSAGYGDEWMKQNGFKGWRTSVGDVTAAAKWM
                     VGQGIADPKRLAIVGWSYGGYAALQSAVTEPQLFKAVVAVAPVTDLAMLKLKARGFTN
                     SELVEEFVGSGPHVREGSPLQNAERISAPVLMFHGERDINVDVDQARAMDRALRGRNA
                     RSELVVFPGLEHSLEDSAARATMLQRIAGFLGQATGNAAGSQ"
     gene            complement(7778..8392)
                     /gene="rpsD"
                     /locus_tag="VF589_11915"
     CDS             complement(7778..8392)
                     /gene="rpsD"
                     /locus_tag="VF589_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX8258328.1"
                     /translation="MSKRSSAKYKLDRRMGENVFGRPKSPVNKREYGPGQHGQRRKGK
                     MSDFGIQLRAKQKLKGYYGDVTEKQFKKTFTEASRMKGDASQNLIGLLERRLDMVVYR
                     AKFAPTIWSARQMVSHGHIRVNGVKCNIASRRVNVADEITLGSKAQEMALVMEAQGLA
                     ERDIPDYVAPDGTAKVTYIRVPTLDEVPYPVKMEPNLVVEFYSR"
     gene            8668..9006
                     /locus_tag="VF589_11920"
     CDS             8668..9006
                     /locus_tag="VF589_11920"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX8258329.1"
                     /translation="MTASRVKPADCTNMAEVRRGVDALDEEIVARLGERFGYMAAAAR
                     IKPSRGAVRDEARKAEVIANAVRHAAAAGIPEPVIAALYERLVEASIAYEMERFDARM
                     AGGSGPAPGA"
     gene            complement(9227..9553)
                     /locus_tag="VF589_11925"
     CDS             complement(9227..9553)
                     /locus_tag="VF589_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258330.1"
                     /translation="MKTRIIIALVSATLCSGAAIAADSTTTDGAKPRRAGTSGITLDP
                     GAATAAESKNRNSKDPNEIVCRRSNDHSSRLDRKKVCMTRAQYEERRMLDRDMVERSQ
                     ASRSTR"
     gene            complement(9764..10084)
                     /locus_tag="VF589_11930"
     CDS             complement(9764..10084)
                     /locus_tag="VF589_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258331.1"
                     /translation="MKTHLIVGLASLLCLTGAAYAAETPTDAVAHTQSGITLDPGEST
                     KNDGKASNPRDPNAIICKRTTDLGTRLPRKKVCMTRAQHTERRLLDRDMVERSQASRP
                     INER"
     gene            complement(10258..10509)
                     /locus_tag="VF589_11935"
     CDS             complement(10258..10509)
                     /locus_tag="VF589_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258332.1"
                     /translation="MKSHLILGLVSVALMSGAAVAGDSKKRTAAKDPNEIVCKTEAET
                     GSRLARKKTCMTRAQMEEQRMINRDMIERSQASRTTTGG"
     gene            complement(10631..11752)
                     /locus_tag="VF589_11940"
     CDS             complement(10631..11752)
                     /locus_tag="VF589_11940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HEX8258333.1"
                     /translation="MSFWTYILRLSNGIYYTGHTDDLDRRMAEHRSGAIKGYTHDKRP
                     LEFMWAEEFPTRAEALEAELRIKKWSRAKKEALIAGNWERRSEAAIPPREREDRASAA
                     LGGAPRLRSGRTDEDGGLHVSNAELVSAAEGHSRPPPAIVLVRPQLGENIGKTARAML
                     NFGLSDLRLVAPRDGWPNPSAGPAASGADIVLERARVFETVAEAVADCRFVYATTVRK
                     RGLVIPVVTPEQAAAEIRAQDGQSAILFGAERSGLETEEAAIAQKILTVPVNPEFRSL
                     NLAQAVILVAYEWSKHERLAVPTEGEPAEPRASQAQLEGLIGQLDEALAKSGYYFPPD
                     RVPATRNTMRTILTKAGWSNREIQALRGMIRALVEPRRR"
     gene            complement(11886..12344)
                     /gene="nrdR"
                     /locus_tag="VF589_11945"
     CDS             complement(11886..12344)
                     /gene="nrdR"
                     /locus_tag="VF589_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX8258334.1"
                     /translation="MRCPFCSHEDSQVKDSRPSEDGASIRRRRQCEDCGARFTTFERV
                     QLRELTVLKSENRREPFDRAKLERSVAIALRKRPVPQERIDKLVSSIQRQLETGGESE
                     VASTAIGERVMDGLKALDPVAYIRFASVYKDFREARDFEEFAGTVGSDKP"
     gene            complement(12494..13810)
                     /locus_tag="VF589_11950"
     CDS             complement(12494..13810)
                     /locus_tag="VF589_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX8258335.1"
                     /translation="MSSRPANDHHDVQHEGFFSQGLGQADSAIAEAIAAELDRQQNQI
                     ELIASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYQGCAPSDAVETLAIERAKQLFGC
                     GFANVQPHSGAQANGAVMLALTKPGDTILGMSLDAGGHLTHGAKPAMSGKWFNAIQYG
                     VRKEDDLIDFDQVEALAKEHKPRLIIAGGSAYPRHIDFARFRAIADEVGAVFLVDMAH
                     FAGLVAAAEHPSPFGHAHVVTTTTHKTLRGPRGGMVLTDDEDIAKKINSAVFPGLQGG
                     PLMHVIAAKAVAFGEALQPGFKSYARAVIANAKALAGRLQERGADLVAGGTDTHLALV
                     DVRPFGITGRDADEALERSAITCNKNGIPFDPLPPIKTSGIRIGSPAGTTRGFGVQEF
                     RDIGDMIADVLEGLRDHGHDGNGEVEAGVKTRVRELCRRFPIYPEA"
     gene            complement(13807..14265)
                     /gene="rpiB"
                     /locus_tag="VF589_11955"
     CDS             complement(13807..14265)
                     /gene="rpiB"
                     /locus_tag="VF589_11955"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX8258336.1"
                     /translation="MADRIAIASDHAAVELKAALAEHLRAGGHEVLDLGPAGADPVDY
                     PDYGYRLAEAVASGEADKGLAICGSGIGISIAVNRHPGARAALVSEPLSARLAREHND
                     ANVIAMGARLIGINMAKACLDAFLGATYEGERHRRRVEKLSNPSFAKEPA"
     gene            complement(14511..14927)
                     /locus_tag="VF589_11960"
     CDS             complement(14511..14927)
                     /locus_tag="VF589_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258337.1"
                     /translation="MFRLRERRLPKAGRPGAYFRVSRDWRLTVTSAFALGDRKALRLL
                     PGMDRIDFGMAEPVRQAVTDLLHLAPFLAERRGRKGGEARVHEIGRLRNLLDLDPGRP
                     EAGERVGEGLDLALAPGLEDVVGLDVVEVRPGLSKC"
     gene            14939..15538
                     /locus_tag="VF589_11965"
     CDS             14939..15538
                     /locus_tag="VF589_11965"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8258338.1"
                     /translation="MQLSAGYDSLPGMNRPGQRFVSEGAAPARFTAAEFMRMAELGAF
                     ADMKVELDHGELVRMTPPMSDHGAVQARVIIALSAVAARGGFTVYGEVGINLGEDTIR
                     ACDAALVRGEVPAHRLLEAAEVELAVEVSASTLDVDLGEKLRDYAAAGIPLYWVVDTG
                     ARVVHVMRSPQGKAYAAREVVRFGEPLELPGGAGTILLD"
     gene            complement(15542..16756)
                     /gene="hemA"
                     /locus_tag="VF589_11970"
     CDS             complement(15542..16756)
                     /gene="hemA"
                     /locus_tag="VF589_11970"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX8258339.1"
                     /translation="MDYQRIFAQAIDRLHAEGRYRVFIDILRNKGSYPNARCFAGHNG
                     PKPITVWCSNDYLTMGQNPKVVAAMEEALHDVGAGSGGTRNIGGNTHYHIELETELAD
                     LHGKEAALLFTSGYISNEATLSTVAKLLPGCVIFSDELNHASMIAGIRNSGCEKKVFR
                     HNDLEHLESLLAAADPEAPKLIAFESVYSMDGDVAPIAAICDLADQYNAITYLDEVHA
                     VGMYGPRGGGISERDQVADRVTIIEGTLGKAFGVMGGYIAAERNIVDVVRSYAPGFIF
                     TTSLSPVLVAGALASVRHLKASSAEREAQQAHAARLKALFAEAGLPVMPSTTHIVPLM
                     VGDPVKAKRISDILLAEYGIYVQPINYPTVPRGTERLRFTPGPAHDETMMRELATALV
                     EVWDRLELRLAA"
     gene            complement(16835..17632)
                     /gene="murI"
                     /locus_tag="VF589_11975"
     CDS             complement(16835..17632)
                     /gene="murI"
                     /locus_tag="VF589_11975"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX8258340.1"
                     /translation="MRMPKPLLIFDSGMGGLSVLQPIRALIRGAPIVYLADSAGYPYG
                     TKSEAEIAARVPALLGRAAERFDPELIVIACNTASTIALDAVRAALDLPVVGTVPAIK
                     PAAALSKSRVIGVLGTPATVRQPYVDRLSEDFAADCLVLRHGSAELVDLAEASLRGES
                     GDPDDYRRILDGLYGQPGGERLDTIVLACTHFPLVEAELAAAAPRPVAFVDGKEGIAR
                     RTAWLTRHLDWPAEPAAGRAVFTRRSPRVEAYRAGLARFGLDRIECL"
     gene            17742..18347
                     /gene="plsY"
                     /locus_tag="VF589_11980"
     CDS             17742..18347
                     /gene="plsY"
                     /locus_tag="VF589_11980"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX8258341.1"
                     /translation="MNLGLYAEPLLSLLLGYLLGSIPFGLLLTAAAGAGDLRRIGSGN
                     IGATNVLRTGRKGLAALTLALDGAKGAAAVLIAAVLFPGLETLAATAALLGHLYPVWL
                     RFRGGKGVATFFGIMAALHWPSSLVFASGWLIMLGLTRYSSVAGMSAALIAPAAAAVM
                     GRFDLALLYLGFGLLVFWKHRANLARLLEGAEPRVGASRDG"
     gene            18340..19440
                     /gene="dprA"
                     /locus_tag="VF589_11985"
     CDS             18340..19440
                     /gene="dprA"
                     /locus_tag="VF589_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX8258342.1"
                     /translation="MAEPAASEDQIARLRLIRSDNIGPITYFQLLARFGSAGAAIEAI
                     PDLAARGGGRAPRLAPRAAIEREIEAVARLGGRYLFLGQGLYPPLLAELESAPPALIV
                     KGRLTLLEKPAVAMVGARNASAAACRFARALAQRLGEAGVVIVSGLARGIDTAAHDGS
                     LETGTIAVIAGGIDISYPPENEARQAAIAERGLLVAEQPPGVEPRARHFPYRNRIIAG
                     LAHGTVVVEAAPKSGSLITARYATEFGRDVMAVPGSPLDPRAQGCNQLIREGAILIQT
                     AEDVLEAISPFNLRPVRQPPLDYRSPAAAADASEQERRTIADLLSPTPVPVDEVVRQS
                     GFAPATVQTVLLELELAGRLERHAGGKVSLAG"
     gene            19530..20633
                     /locus_tag="VF589_11990"
     CDS             19530..20633
                     /locus_tag="VF589_11990"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX8258343.1"
                     /translation="MSEPRPSPERNGDAARLLLAAARERFSVAATDLLLPEASRLTQW
                     QRITAAALLVRLVRTAEDALRTSLADQFRGHEALHAAFSSAHVPIALPLLERAQVLRD
                     AELGTVLVRRVEEHRFWKEQADAAADRSELMLALVRDEDAAVASLAMAVTIARSRRFD
                     QFHEPVMGETELPAEIQHRLVWMIAAALRQYMIQQHGLSPGEADEGIAAAAGELIRGY
                     DEGDSLEAACTRLARRLDQTERLDDATLARFLEEGQLPLFIAAIGVRAALDYAATWEV
                     LSDPRGRGPALLLRSAGVGRDHAANILLILNARGRMFSGSEGDAAAAQLDLFEATAEA
                     EARQVLRLWQVDPGYRAAIARLSTRARPAPDAA"
     gene            20630..22027
                     /locus_tag="VF589_11995"
     CDS             20630..22027
                     /locus_tag="VF589_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX8258344.1"
                     /translation="MTALVQPDPAVIRGRLDGEGRLVEADPPLARLHHRAGGRQGGAL
                     AVPQVAALARLARRLGIVISRGAIAADGIRDIDLWVRAAPVGDGVEIVIESWTERPAR
                     GPADAPDQERQADFLRASADWMWETDESLRFTTLSPAAAAAVGKIPAELVGKQLTRLF
                     RFHEGEDGALPILNALAEQRRFDDQYAELRSGNRGRYRLSGVPLIDGTGRFAGFRGAA
                     ASLPRWLPDVANDPAPPPPTKDSPAFGERLDKALREPLDHIIANAETIGAQPDGPLTP
                     DYAGYAQDIAVAGRHLLALVDDLIDLQAIERPGFRPDAEPIDLAEIARRAAGLLSVRA
                     AEKQVRIDRPADDESLPATGEFRRALQILMNLLTNAIRYSPDGAQVWIRTEREGDLAC
                     VIVADQGKGIAEADQARIFDKFERVDVTEPGGTGLGLYIARRLARLMGGDIAVDSAPG
                     QGARFTLTLPIGPES"
     gene            complement(22032..23015)
                     /locus_tag="VF589_12000"
     CDS             complement(22032..23015)
                     /locus_tag="VF589_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX8258345.1"
                     /translation="MEFLTLDFLGKPVWMWLGFMGIVAALLALDLGVLHRKQREIGVG
                     ESLGLSAVYVALGLAFGGWVWWYMGETAGMNYLTGFVVEKSLAMDNVFVIAMIFTYFA
                     VPRLYQHRVLFWGILGVIVLRAVMIGVGAALVEQFGWLLYIFAAFLIFTGIKMWAMAD
                     REYDVAGSPVLKWAYRRFRVTEGLHGERFWVRQPDAASGRLAWHMTPLFLALIMVEFV
                     DVVFAVDSVPAIFAITTDPFIVYTSNIFAILGLRALYFALSAMVARFHYLKYALALLL
                     VFIGSKIFVADALGLGKIPPAISLGITFAILAGGTLFSMWKTAPPRAAEAA"
     gene            complement(23159..23329)
                     /locus_tag="VF589_12005"
     CDS             complement(23159..23329)
                     /locus_tag="VF589_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX8258346.1"
                     /translation="MHPFAYRLSVIHHKLDDEIRRELKRRAPDWIRLLRLKKLRLAVK
                     DRLHGHLAATRA"
     gene            complement(23506..>23998)
                     /locus_tag="VF589_12010"
     CDS             complement(23506..>23998)
                     /locus_tag="VF589_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8258347.1"
                     /translation="KRFLRHLRPYWDVHRHRIAPAVAERLERLRAEGRLEIVPGRVAG
                     VAREADAMAVTIRRRGGGGEAVRKVAAVINCTGPSGDIAGADDALVAQLLASGMARPD
                     PLRLGFEVDENSRVIAADGSVRGNLFAVGPITKGCFWEIVAVPDIRLQVQAAAAEMLR
                     AGP"
BASE COUNT         3678 a         8208 c         8194 g         3918 t
ORIGIN      
        1 gtggtgagcc ctgctgggtt cgaaccagcg acctactgat taaaagtcag ttgctctacc
       61 gactgagcta agggcccttg cgcgctccct atggtcgggc gcggcggcgg tcaaccgggc
      121 ttggccgcgc gagccgggcg cggagctgtc ggacggtgag gccggtgagc ggatcgcgcc
      181 agagcgggac gagctcggcg agcggatcga gcacgaagaa gcggcggcga aattccgggt
      241 gcgggacgat caggccgggg ccggcatagg cgccttcgga ccagaggatg atgtcgagat
      301 cgatcacgcg cgggccccag cggcggccgg gccggcgccc gaagccgcgc tcgatccgct
      361 tgaggagggc gagcagggca ggggggtcga gggagctgtc gaggatcgcc gcggcattgg
      421 cgaagccgcg gccggcgggg ccgagcgcgg gcgtcgcgcg gatgcgggag aggcgggcga
      481 cgcccaatgc cgcggcggcg gcgcgcaccg cggccgcggg gcttccgtgg cggcccgggc
      541 ggttggagcc gagggcgatg gcgtagagcg atgatggaat tgttatgaac tccgttcgcc
      601 ctgagcttgt cgaagggctg cccttcctct ccggctccct aaggataagg agagggcttc
      661 gacaagctca gcccgaacgg tttagtgacg caagatgtac gaacccatat cccccgcccc
      721 cgccgccgag ccgccgcgcg attgtccgct ttgcccgcgg ctggtcgcgt tccgcgaggc
      781 attgcgggcc gagcatccgg attggtggaa cgcgccggtg ccgaatttcg gcgaccccga
      841 cgcctggctg gtgatttgcg gcctcgcgcc cggcaagcac ggcgccaacc gcaccggccg
      901 gcctttcacg ggcgattatg cgggcgtgct gctctacgag accttgctga agttcgggct
      961 cgccgagggc cgctatgagg agcggcccga cgacagcctg cgcctgaagg gcgtggcgat
     1021 cgtcaattcg gtcaagtgcc tgccgccgca gaacaagccg acgccggagg agagcgccac
     1081 ctgccggagc tatttcacgc tggcgctggc ggcgctcggc aacgcccgcg tccaggtcgc
     1141 gctcggccag atcgcgcacc aggcggcggt gcgcaccgcg ggcgggcggc tcgccgacta
     1201 taggttcggc cacctcgccg agcatcgcct gcccgacggg cgcatcctgg tcgatagcta
     1261 ccattgctcg cgctacaacc agaatacggg gcggctgacg gcggcaatgt tcgaagccgt
     1321 gttcgcgcgg gcgctggagc tgcggccttg atcaaccgcg gattgtaacg tgatcagcca
     1381 agcggactcc cttccgaagc tcggtgccat aagtgtggct tatcccggct ctctcggcga
     1441 gtagcttgag attgacacct ttcaaacggg ccgcgtgccg aatgccaaag aggtggatcg
     1501 tcacgacccg gtcgtttttc ggagcagcct tacaagcagc ccttagctcg tccacgagtt
     1561 cattcatcga cctctccgtt caacaacatt gtcaaatatc ctgcgtcagc cggccgtaaa
     1621 gctccgggcg gcggtcgcgg aagaagccga aggcggcgcg gtgggcgcgg gcctgggcga
     1681 ggtcgagcgt ggcggtcagc acgccggtct cctcggcgcc gaattcggcc aatatgtcgc
     1741 cgcgctggtc gcagatgaag ctgtggccgt agaaacgctg gccgtgctcg gtgccgatgc
     1801 ggttggcggc gacgacgggc accacgttcg acaccgcgtg gccgatcatc gcgcggcgcc
     1861 acatccggct ggtgtcgagc gtgggatcgt gcggctcggc gccgatcgcg gtcgggaaca
     1921 gcaatatctc cgccccgtcg agcatcagcg cccgcgccgt ctccggatac cattggtccc
     1981 agcagaccgc cgggccgacc gtgccgaacg gcgtcttgta ggtcttgaag ccggtattgc
     2041 cggggcggaa gtagaatttc tccgaatagc cggggccgtc ggggatgtgg ctcttgcgat
     2101 agacgcccat caccgcgccc tggtcgtcga tcatggcgag gctgttataa tgatggtggc
     2161 catgggcttc gaagaagctg gtcgggatat agacgcgaag ctcggcggcg agcgcgcgca
     2221 tcgccagcac ggccggatgc gcgtcggcgg acagcgcgcg ggtgaaatgc gcgtcgtcct
     2281 gggtgcggca gaagtaatgg ccttcgaaca attcgggcgg gaggatcagc ttcgcgccct
     2341 tgccggcggc ctcgcgcacg agctccgaga cggcggcgat attggcgtcg atatcgtcgc
     2401 tgaaggcgag ctggagggcg gcgacggtga tctcagacat gttcatcatc cttatccgcg
     2461 ctcaggggtg cgggcctttc cccaaaccgc tcatcctgag gagggactga gccttggcga
     2521 aggcccgtct ccaaggacca ttgtgcgtcc ttcgagacgc cggttcgaca ggctcaccgg
     2581 ctcctcagga tgagcgggtg tgggtcgtca caacctagtg cattcccccc cctaaaccgg
     2641 catttgctgg ctgatgcagt ggaagctgcc gccgccgatg aggacgtggt cggcgcgcag
     2701 gcccagcgcc ttgcggccgg ggaacaaagc gccgatcgcg tccaccgccg ccgcgtcgtt
     2761 ggccgcgccg tagaccggca ccgccaccgc cgcattgccg atgtagaagt tcatatagga
     2821 agccggcgcg atctcgccat cccgctcaat gaggcccggc gaggggaggg ggaccacctc
     2881 caccccgaac gcgcgcgcgc gagccgcggc gtcggcatag atcgcggcat tggggtcatc
     2941 ggcgccggcc ggcaccggga tcgccagccg gttggcgccg acgaaacggg cgatattgtc
     3001 gacatggccg tcggtatggt cattgagcag cccttcgccg agccacagca cgcgctcgag
     3061 gccgaggtcg gccgccagcc gcgcttcgac ctcgtgcctg ccgaggccgg gattgcggtt
     3121 cgggttgagc aggcattgct cggtggtgac ggcgaggccg gtgccgtcga cgtcgatcgc
     3181 gccgccttcc aatatccagt cgtggcgccg cgcgtcgagg tcggcggttt cggcgagccg
     3241 caggccgatc gtgtcgtcgc cttccagatc atatttgccg ccccagccgt tgaagcgaaa
     3301 gctcgccgcc gccggcatgc cgtggtcctt caggatgatc ggcccggtgt cgcgcagcca
     3361 gatatcgccg aacggctcct gcaccacctc cgcccacggc gccagcgccc gcgccgcctc
     3421 ggccgccttc gggtccgccg ccaccagccg cacctgctcg ccggcgccgc ccgcatgcac
     3481 cagttcggca aaggcggcca cttcggcccg cgcggcgtcg agatcctccg accagaggtc
     3541 ggcatggctg ggaaagccga tccacaccca ttcgtgcggc gcccattcgg ggggttggcg
     3601 cgacgtcatt gggctcattg cgcgccttgg gctccggcgc gcgacttgtc gcggatggcg
     3661 cggaattcgt cgccttcgta ccagttcggc caggcgtcgc tcatcgccag ctcgcgcccg
     3721 atccggtaat agaggccgag gtcgcggatc gcgcccgacc agttccaatt ggggtcatat
     3781 tcgtcgctcg gctggtggta gcggttggcg cgataatcct cggccgccgc ccggccggcc
     3841 gcgacgccgc cctcgaccag atcctcgccg ccctcggcgt acagcatcgg cacgccgaac
     3901 ttggcgaggc tgaaatggtc ggagcggtaa taatagccgg cctcgggcgt cggctcgttc
     3961 ttgacctcga gcccgccgcc ggcgcggcgc agataatctt cgagctcgga cttgcccatc
     4021 ccgatcacga ccaggtcgcg ggtggtgccg gcgacgttga gcccgtccat gttgatcccg
     4081 cccgccgtct gcgcgtgcgg atagaccggg ttctcgccat aatatcgcga tccgagcagc
     4141 cccgattcct cggcggtgac ggcaaggaag atgatcgagc ggtcgggcgc gcccgctttg
     4201 gcgtgggctt cggcgagcgc gatcaggccg gcggtgccgg tggcgttgtc gagcgcgccg
     4261 ttgcagatgt cgtcgccggt cgcatcggcg tcgcagcggc cgagatggtc ccaatgggcg
     4321 gtgtagatga catattcgtc cgggcgggcc cggccgcgca ggatgccgat cacattcttc
     4381 gattcctgcc gcttgatctg gttggtgagc gagatcgacg cctggctgcc gagcgagacc
     4441 gccttgaagc ccttctgccg ggcggccgcg ctcagccgcg cataatcctg gccggcattg
     4501 gcgaacagcc gctgggccgc ctcgcgcgtg atccagccga tcgccttcga ctggtccata
     4561 tggttgtcgg gcgcgtccat attatattgc gggccggtcc acgatgattg caccacgttc
     4621 cagccatagg cggcgggctc ggtctcgtgc acgatgaagg cggcggcggc gccctgccgc
     4681 gcggcttcct catatttgta ggtccagcgg ccgtaatagg tcatcgcccg gccgccgaac
     4741 ggcccctcca gcccctggct ttgataatcg gggtcgttga cgaggatgat caccgtcttg
     4801 ccgcgcacgt ctagcccggc atagtcgttc cagccgcgct cgggcgcgtt gatgccgtag
     4861 ccgacgaaca cgatctcgct gtccttgaga tcgatccgcg gctgcaattg gtagctgttg
     4921 gcgacgaaat cggtgcggta ggcgaaggag agcggcgcct tgccgccggc gacggtgagc
     4981 gcggtcgggc tggcggtgtt ctcggccagc ggcacattct ggtaccagct tcccttgttg
     5041 cccggctcga gcccggcccg ctcgaaccgc tcggcgatca gccgcaccgt cttttcctcg
     5101 ccggccgtgg tcggcgcgcg gccttcgaat tcgtcggagg cgagccgttc ggtcaccgtc
     5161 cgcagcgtct cttccgagag cgcgggttcg gccgccccgg cggtcgccgc gggccgcggg
     5221 ccggtggtcg tgcagccggc cagcgcgagg gcggcgcctg cggcggtcat gatgcggaaa
     5281 ggcatggctt ttctcctgcc ggggccgagc cccccaagtg cggcctcttg gcagcgcgtg
     5341 aacggctccg caagttccgg caggcgaatt tgatgcttgc cctgaggctc ccttcatggt
     5401 ctcttgtgcg ttgcaaaatt gggggattac atgctgaaaa agttaatcat tccggccgtt
     5461 gccggagccg cggcgttgca ttcgggggca gccgcgcaac aagccgcgcc gtcggccacc
     5521 atgttcggcg cgcgcgaggc ggtcgagagc atcgacatct cgccggacgg cacgaaattg
     5581 gtctatctgg cgccggccgc gggcgcctct tcggtcgctt tggtcggcga ccttgccggc
     5641 ggcaaacccg ccgtcgtcgc cacgtcgagc ggcaagcccg accggctgag ctggtgccgg
     5701 ttcgtcagca acagccgcat catctgccgc gcctatgccc tcatcgacaa taagggtgtc
     5761 ctcatcccgt tcgggcggct gttcagcatg gacgccaatg gcggcgacat gaaggagctg
     5821 ggccagagcc tgtcttattt cgactcccgc atccgccagt tcgacgggtc gatcctcgat
     5881 tggatgccgg aggacaacaa cgccgtgctg atggcccgcg actatatccc cgaggagggt
     5941 cggatcggca cccggctggt gcggcagaag gacgggctcg gcgtcgaccg gatcgacgtg
     6001 cgcaccttga aagcgacccc ggtcgagccg gtcagccgga cggcgtcgga tttcatgacc
     6061 gacgggcgtg gcaaggtccg catccgcgag atccagaacc gccgcgccgg cgcctccgac
     6121 cagctgatgg cgcagacgat cagcttttcc taccggcgca ccggccggtc cgactgggag
     6181 gacttctcca cctacaatat gatgaaccgc gaaggcacct atccggtcgc gatcgacgcc
     6241 tcgctcaacg ccgcctacgc gctgcgcaag ctcaacggcc gcttcgccct ctaccgggtg
     6301 aagctggacg gctcgatggc cacggaattg gtctatgcca atcccagggt cgacgtcgac
     6361 aatgtcgtcc gtatcggccg cggcggccgc gtcatcggcg tcaccttcgc cgaggaggcg
     6421 cgcagcaccg tctatttcga tccggaatat gccaagctcg ccaaatcgct gtcgaaggcg
     6481 attccgaacc tgcctttggt ccgcttcgcc gggtcgagca acgacaataa caaactgctg
     6541 atcttcgccg gcagcgactc cgatcccggc cgctattatg tctatgacaa gaaggcgcgg
     6601 gcgctcaacg aggtgatgct cgcccgcccg cagctcgaga atatgaagct cgcgacgatg
     6661 aagccggtca cctatgcggc cgccgacggc gtccagatcc cggcctatct ctcggtcccg
     6721 cccggcaagg accccaagaa cctgccggcg atcgtgctgc cgcatggcgg cccgacggcg
     6781 cgcgacgaat ggggcttcga ctggctcacc caattcctcg tcgcccaggg ttatgccgtg
     6841 ctccagccca attatcgcgg ctcggccggc tatggcgacg aatggatgaa gcagaacggc
     6901 ttcaagggct ggcggacctc ggtcggcgac gtcaccgccg ccgccaagtg gatggtcggc
     6961 cagggcatcg ccgatccgaa gcggctggcg atcgtcggct ggtcctatgg cggctatgcc
     7021 gcgctgcaat cggccgtgac cgagccgcag ctgttcaagg cggtggtcgc ggtcgccccg
     7081 gtcaccgacc tcgccatgct caagctcaag gcgcgcggct tcaccaattc ggagctggtc
     7141 gaggagttcg tgggctccgg gccgcatgtc cgcgagggct cgccgctcca gaatgccgag
     7201 cgcatctccg ccccggtgct gatgttccac ggcgagcgcg acatcaacgt cgacgtcgac
     7261 caggcgcggg cgatggaccg ggcgctgcgc ggccgcaacg ccaggagcga gctggtcgtc
     7321 ttcccgggcc tcgagcacag cctcgaggat tcggcggcgc gcgcgaccat gctccagcgc
     7381 atcgccggct tcctcggcca ggccaccggc aacgccgccg gctcacaatg aggtggttcc
     7441 cctgagccac ggctcagggg gaatcgctcg ttcaggctgg agcttcagct gacctgttcc
     7501 ccggcgaagg ccggggccca gactccagcg cagctggacc cccgctttcg cgggggagcg
     7561 aaaaattcgg cgttcaaggt cgttccggga gcgccaccct gtccgcggcg aaggccggcg
     7621 tccagccttc cgtgccgctg aaccctgact ttcgcggggg agcgaagccc cggacgcccc
     7681 cgcgccgccg gcgcgcaata aaaagccccc cggagcaggg ctccgggagg ctggtcgaaa
     7741 gctttcctcc ccatgcaccg catggggggc tcccacgtta gcgcgaatag aattcgacga
     7801 cgaggttggg ctccatcttc accggatacg gcacttcgtc gagcgtcggc acgcggatgt
     7861 aggtcacctt ggcggtgccg tcgggggcga cgtaatcggg gatgtcgcgc tcggcgagcc
     7921 cctgggcctc catcaccagc gccatttcct gcgccttgga gccgagcgtg atctcgtcgg
     7981 cgacattgac ccggcgcgag gcgatgttgc acttcacccc gttcacccgg atgtggccgt
     8041 ggctcaccat ctgccgcgcg gaccagatgg tcggcgcgaa cttggcgcgg tagacgacca
     8101 tgtccagccg gcgctcgagc aggccgatca ggttctgcga agcgtcgccc ttcatgcggc
     8161 tggcctcggt gaaggtcttc ttgaactgct tttcggtgac gtcgccataa tagcccttga
     8221 gcttctgctt ggcgcgcagc tggatgccga aatccgacat cttgcccttg cggcgctgcc
     8281 cgtgctggcc ggggccgtat tcgcgcttgt tgaccgggct cttggggcgt ccgaagacgt
     8341 tctcgcccat gcggcggtcg agcttgtact tggcgcttga gcgcttcgac atgatgatac
     8401 tccaatctgc ttcgttgtcg tggcagcccg cacggcccgc ggccccggtt ctggaaccgg
     8461 atcccgcttg tcgccgcgag ggctgttgtt tcccggaacc gcaccgccgg cacgaggggc
     8521 cggcgcggct gccgcttcac cggggtgcgg agccaattgc gaagcgcgcg acatggccgc
     8581 tcaggcgctc caagtcaacc tcggcggcgg cccggcccgc gttccaggcc ggctcgacag
     8641 cggccttggg cgcgctaaag aggcgcgatg acggcatcca gagtgaaacc cgccgattgt
     8701 accaacatgg cggaggtccg ccgcggcgtc gacgcgctcg acgaggagat cgtcgcccgg
     8761 ctcggcgagc gtttcggcta catggccgcc gccgcgagga tcaagccgtc gcgcggcgcc
     8821 gtccgcgacg aagcccgcaa ggccgaggtg atcgccaacg ccgtccggca tgcggccgcc
     8881 gccggaatcc ccgaacccgt catcgccgcg ctctacgagc ggctggtcga agcgtcgatc
     8941 gcctatgaga tggagcggtt cgacgcgcgt atggccggcg gatcggggcc ggcgcccggc
     9001 gcctgatcga aggctcgaaa gaagctgcgg cttcgcgcgg ccctggctgc gaccagcgct
     9061 ccttgctgcg accagcgctc ctggctgcga ccagcgcaag gaacagaggc gcgacgctcc
     9121 caacgcggtc tgactatgat gatggcggcc gtgacttcac gcccttgccg ggcacaccgc
     9181 ggacgcttcg aactccggcg gcgtttcgac ctgggacagt cgggccttag cgcgtgctac
     9241 ggctggcctg gctgcgctcg accatgtcgc ggtccagcat gcgccgctct tcatattggg
     9301 cgcgggtcat gcacactttc ttgcggtcaa gtcgcgagct gtggtcgttg ctgcgcctgc
     9361 agacgatctc gttcggatcc ttgctgttgc ggttcttgct ctcggccgcg gtggcggcgc
     9421 cgggatcgag cgtgatgccg ctggtgccgg cccggcgcgg cttggcgccg tcggtggtgg
     9481 tgctgtccgc ggcgatggcg gcgcccgagc acagggtggc ggagaccaga gcgatgatga
     9541 tgcgcgtctt catgacttca ttccttcgat gaagtaaacc ggcctggatg ttatacacca
     9601 gatcggcccc tctggaaagt ggaacgctgc ggccgcaggc ggttgccggg cgtggcgcaa
     9661 ggcctcccgg ggggcaggta ccgccgtctg ccggagcaaa ggggatgcca gccggcgctt
     9721 cccggtcggc agcgccgccg cgaagctcgc ccggaggccg cctttagcgt tcgttgatcg
     9781 gacggctcgc ctggctgcgc tcgaccatgt cgcggtcgag cagccggcgc tcggtatgct
     9841 gggcgcgcgt catgcagacc ttcttgcggg gcaggcgcgt gccgaggtcg gtcgtgcgct
     9901 tgcagatgat cgcgttggga tcgcgcgggt tgctggcctt gccgtcattc ttggtcgact
     9961 cacccggatc gagcgtgatg ccgctctggg tgtgcgcgac ggcgtcggtc ggggtctcgg
    10021 ccgcataggc ggcgccggtg aggcagagga gcgatgcaag gccgacgatc aggtgcgttt
    10081 tcatgacaac ccctttccaa agcaacgcgc catcctaatg gaatggaggc agaatgtaaa
    10141 cgcgccgaat ccggcgcgca cgcgatcggc ggcttccgct agcggcggaa actgctgcag
    10201 aacgggggag ggcgagcatg gccgccctcc gatgatgctg gcccgccaaa gcgggaatta
    10261 gccgccggtg gtcgtgcggc tcgcctgcga acgctcgatc atgtcgcggt tgatcatgcg
    10321 ctgctcttcc atctgcgcgc gggtcatgca ggtcttcttg cgggcaaggc gcgagccggt
    10381 ctcggcctcg gtcttgcaga cgatctcgtt ggggtccttc gcggcagtgc gcttcttgct
    10441 gtcgcccgcg acagccgcac ccgacatgag agcaacggaa accagaccca aaatcagatg
    10501 cgacttcatg atgagctttt ccttcactta cgaatagttc cccaattggt cggccaatac
    10561 acgttgttgg tcgaaaagta aagtcgcggg caagcgccgt cttccgccgc gtcggcgtga
    10621 gatcaacgac ttagcggcgg cgtggctcga cgagcgcgcg aatcatcccg cgcagcgcct
    10681 ggatttcgcg gttcgaccag ccggccttgg tgaggatggt gcgcatcgtg ttgcgggtcg
    10741 ccggcacccg gtcgggcggg aaataatagc ccgatttggc gagcgcctcg tcgagctgcc
    10801 cgatcagccc ctcgagctgg gcctggctgg cccgcggctc ggccggctcg ccttcggtcg
    10861 ggaccgcgag gcgctcgtgc ttcgaccatt cataggccac caggatcacc gcctgggcga
    10921 ggttcagcga gcggaattcg gggttgaccg gcaccgtcaa tatcttctgc gcgatcgccg
    10981 cttcctcggt ttccagcccg gaccgctcgg ccccgaacag gatcgccgac tgcccgtcct
    11041 gcgcccttat ctcggccgcc gcctgctccg gagtcaccac cgggatgacc agcccgcgtt
    11101 ttctcaccgt ggtggcatag acgaaccggc agtccgccac cgcctcggca acggtttcga
    11161 acacccgcgc ccgctcgagc acgatatcgg cccccgacgc ggcgggtccg gccgaagggt
    11221 tcggccatcc gtcgcgcggc gcgacgaggc gcaggtcgct aagcccgaaa ttgagcatcg
    11281 cgcgggcggt tttcccgata ttttcgccaa gctgcgggcg gacgaggacg atggcggggg
    11341 ggggtcgcga atggccttcg gctgcactta caagttcagc gttggataca tgaagaccac
    11401 catcttcatc cgttcgcccc gagcgaagtc gaggggcccc gccgagcgca gccgaggccc
    11461 tgtcctcccg ctcccgcggc ggaatcgcgg cctcgctccg ccgctcccaa ttgccggcaa
    11521 tcagcgcttc cttcttcgcc cgtgaccatt tcttgatcct gagctcagct tccaaggctt
    11581 ccgcccgggt cgggaattct tcggcccaca tgaattccag cggtcgcttg tcgtgcgtgt
    11641 agcccttgat cgctccgctg cggtgctcgg ccatgcggcg gtccaggtcg tcggtgtggc
    11701 cggtataata gatgccgttc gagaggcgca ggatgtaggt ccagaagctc atcggacggg
    11761 gtctcggctg cgctcgacgg ggtgtctcga ctgcgctcga cacgaacgga ttgttaccat
    11821 cggggttggt cggctgctct cgacggggtg tctcgactgc tctcgacacg aacgaatggg
    11881 taccatcagg gcttatcgga tcccaccgtg cccgcaaact cctcgaaatc cctcgcttcg
    11941 cggaagtcct tatagacgct ggcgaagcgg atataggcga ccgggtcgag cgccttcagg
    12001 ccgtccatca cgcgttcgcc gatcgcggtc gaggcgacct cgctctcgcc gccggtctcg
    12061 agctggcgct ggatcgagga gaccaatttg tcgatccgct cctgcggcac cggccgcttg
    12121 cggagcgcaa tcgccaccga gcgttcgagc ttggcgcggt cgaacggttc gcggcggttt
    12181 tccgatttca acacggtcag ctcgcgcaat tgcacccgct cgaaggtggt gaagcgggcg
    12241 ccgcaatcct cgcattggcg ccgccgccgg atcgaggcgc cgtcctcgct cgggcggctg
    12301 tccttcacct ggctgtcttc gtgcgagcaa aaggggcagc gcatatgtca gcttatcgtc
    12361 acaggtccgt tcgccccgag cgaagtcgag gggccctgcc gagcgaagcc gaggccattt
    12421 ctcggcttcg ctcgaaaggg tgtctcgact gcgctcgaca cgaacgggtg ggtgcgcggg
    12481 tacgtgacgg gcatcaagcc tccggataga tcgggaagcg gcggcagagc tcgcggacgc
    12541 gcgtcttgac accggcctcg acctcgccat tgccgtcatg gccgtggtcg cgaaggccct
    12601 cgagcacgtc ggcgatcatg tcgccgatat cgcggaattc ctggacgccg aagccgcggg
    12661 tggtgccggc cggcgatccg atccggatgc cgctggtctt gatcggcggc agcgggtcga
    12721 acgggatgcc gttcttgttg caggtgatcg ccgagcgctc gagcgcctcg tcggcgtcgc
    12781 ggccggtgat tccgaacggc cgtacgtcga ccaaagcgag atgggtgtcg gtgccgccgg
    12841 cgaccaggtc ggcgccgcgc tcctgcaatc gcccggccag cgccttggcg ttggcgatca
    12901 ccgcccgcgc atagctcttg aagccgggct gcagcgcctc gccgaacgcc accgccttgg
    12961 ccgcgatgac gtgcatcagg gggccgccct gaaggccggg gaacaccgcc gagttgatct
    13021 tcttggcgat gtcctcgtcg tcggtcagca ccatgccgcc gcgcgggccg cgcaacgtct
    13081 tgtgggtggt ggtggtgacg acatgggcgt ggccgaaggg ggaggggtgc tcggccgccg
    13141 cgaccagccc ggcaaaatgg gccatgtcga ccaggaagac cgcgccgact tcgtccgcga
    13201 tggcgcggaa gcgggcaaaa tcgatgtgac gcggataggc cgagccgccg gcgatgatca
    13261 gccgcggctt gtgctccttc gccagcgctt caacctggtc gaaatcgatg aggtcgtctt
    13321 ccttgcgcac gccatattgg atcgcgttga accacttgcc cgacatggcc ggcttggcgc
    13381 cgtgggtgag gtggccgccg gcgtcgaggc tcatgccgag gatggtgtcg cccggcttgg
    13441 tcagcgccag catcaccgcg ccgttggcct gcgcgcccga atgcggctgg acgttggcga
    13501 agccgcagcc gaacaattgc ttggcgcgct cgatcgccag cgtctcgacc gcatccgacg
    13561 gcgcgcagcc ctgatagtaa cggcggcccg gatagccctc ggcatatttg ttggtcagca
    13621 ccgaaccctg cgcctcgagc acggcgcggc tgacgatatt ctcggaagcg atcagctcga
    13681 tctggttctg ctgacggtcg agttcggcgg cgatcgcctc ggcgatggcg gaatcggcct
    13741 ggccaagccc ctggctgaag aagccttcgt gctggacatc atggtggtcg ttggcgggac
    13801 ggctgctcat gcgggctcct tcgcgaagct gggattggaa agtttctcga cccggcggcg
    13861 gtggcgctcg ccttcatagg tcgcgccgag gaaggcatcg aggcaggcct tggccatatt
    13921 gattccgatc agccgcgcgc ccatcgcgat cacgttggcg tcgttatgct cgcgggcgag
    13981 ccgggccgag agcggctcgg aaacgagcgc ggcgcgcgcg cccgggtggc ggttgaccgc
    14041 gatcgagatg ccgatgccgg agccgcaaat ggcgaggccc ttatcggcct cgcccgacgc
    14101 gaccgcctca gccagccggt agccgtaatc gggataatcg accgggtcgg cgccggccgg
    14161 gccgaggtcg agcacctcat ggccgcccgc gcgcagatgc tcggccagcg ccgccttcag
    14221 ttcgacggct gcgtgatcgg aagcgatggc gattcggtcg gccacgccgt cagtccccaa
    14281 tgcggttgaa ccacaggctc tagtggaaag ggcgccgctc cgccaccgca gattgatgct
    14341 tgagggccat tctgctgtat tgtagttata acacactgac gggggaataa gcatgaagtt
    14401 gagggcggcg atcggggcgg tcatggcgat ttcagacttc gcgattgcgg gggcgccggc
    14461 cgggcggcgc cggccgcgga gaagccggtc gaagtgatgg tgctcggcac ctaacatttc
    14521 gacaatcccg ggcggacctc aacaacgtca aggccgacga cgtcctccag cccaggcgcc
    14581 agcgcgagat cgaggccctc gccgacgcgc tcgccggctt ccggccgacc cggatcaagg
    14641 tcgagcaggt tacgaagacg cccgatctcg tggacccggg cttcaccccc tttacgcccg
    14701 cgacgctcgg cgagaaacgg ggcgaggtgg agtagatcgg ttaccgcctg gcgcacaggc
    14761 tcggccattc caaagtctat gcgatccatg cctggaagga ggcgaagggc cttccgatca
    14821 cccagggcga aggcggaggt gaccgtcaac cgccagtcgc gcgaaacgcg gaaataggcg
    14881 ccggggcggc ctgctttggg caggcgccgc tcgcgaaggc ggaacatgcg attccgcgat
    14941 gcagctttcg gccggctatg atagtttgcc cggcatgaac cggcccggcc aacgctttgt
    15001 ctccgagggc gccgcgcccg cgcgcttcac tgcggcggag ttcatgcgca tggccgagct
    15061 cggcgcgttc gccgacatga aggtggagct ggaccatggc gagcttgtcc gcatgacccc
    15121 gcccatgagc gatcacggcg cggtccaggc gagggtgatc atcgctcttt cggccgtcgc
    15181 cgcccgaggc ggcttcaccg tctacggaga ggtcgggatc aatctcggcg aggatacgat
    15241 ccgggcctgc gacgcggcgc tcgttcgcgg cgaagtgccg gctcatcggt tgctggaagc
    15301 ggccgaggtt gaactcgccg tggaggtttc cgcctcgacc ctcgacgtcg atctcggcga
    15361 gaagctgcgc gattatgccg cggcgggaat tccgctttac tgggtggtcg atactggcgc
    15421 gcgagtcgtg cacgtcatgc gcagccccca aggcaaagcc tatgccgcgc gcgaagtggt
    15481 ccgcttcggc gagccgctcg agcttccggg cggcgcgggc acgatcctcc tcgactgacg
    15541 ctcaggcggc cagtcgaagc tccagccggt cccagacctc caccaaggcg gtggcgagct
    15601 cgcgcatcat cgtctcgtca tgcgccggtc cgggggtgaa gcgcagccgc tcggtgccgc
    15661 gggggacggt gggatagttt atcggctgca cgtagatgcc atattcggcg aggaggatgt
    15721 cgctgatcct tttggccttg accggatcgc cgaccatcag cggcacgata tgggtggtcg
    15781 agggcatgac cggcaggccg gcctccgcga acagcgcctt gagccgcgcc gcgtgggcct
    15841 gctgcgcctc gcgctcggcc gaggaggcct tcaaatggcg cacgctggcg agggcgccgg
    15901 cgaccagcac cggcgacagc gacgtggtga agatgaagcc tggcgcgtag ctgcgcacca
    15961 cgtcgacgat gttgcgctcg gccgcgatat agccgcccat cacaccgaac gccttgccca
    16021 gcgtgccctc gatgatcgtc acccggtcgg cgacctggtc gcgctcggaa atgccgccgc
    16081 cgcgcgggcc gtacatgccg accgcgtgga cctcgtcgag ataggtgatg gcgttatatt
    16141 ggtcggccag gtcgcagatc gcggcgatcg gcgccacgtc gccgtccatc gaatagacgc
    16201 tttcgaacgc gatcagcttg ggcgcctccg ggtcggcggc ggccagcagc gactcgagat
    16261 gctcaaggtc gttgtggcgg aacaccttct tctcgcagcc cgaattgcgg atgccggcga
    16321 tcatcgaggc gtgattgagc tcgtccgaga agatcacgca gccgggcagc aatttggcga
    16381 ccgtggaaag cgtcgcctcg ttggagatat agcccgaggt gaacagcagg gccgcttcct
    16441 tgccgtggag gtcggccagc tcggtctcga gctcgatatg ataatgggtg ttgccgccga
    16501 tattgcgggt accgccggag ccggcgccga catcgtgcag cgcctcctcc atcgccgcca
    16561 ccaccttcgg gttctggccc atcgtcagat agtcgttcga gcaccagacg gtgatcggct
    16621 tcggcccgtt atggccggcg aagcagcgcg cgttcgggta ggagcccttg ttgcgcagga
    16681 tgtcgatgaa gacgcgatag cgcccctcgg cgtgcagccg gtcgatggcc tgcgcgaaga
    16741 tccgctgata gtccaagtgt ccaaccccgg ggtaaccttg cccgctcata ccggataccg
    16801 gcccgcaaag gccagtgcga acggttcgca acagtcagag gcattcgatc cggtcgagcc
    16861 cgaagcgggc gaggccggcg cgataggcct cgacccgggg cgagcgccgc gtgaacacgg
    16921 ccctgccggc ggcgggctcg gcgggccagt cgaggtgccg ggtcagccag gcggtgcggc
    16981 gcgcaatccc ctccttgccg tcgacgaacg ccaccggccg cggcgcggcg gcggcgagct
    17041 cagcctcgac caaagggaaa tgggtgcagg cgagcacgat cgtgtcgagc cgctccccgc
    17101 ccggctggcc gtagagcccg tccaggatcc ggcgatagtc gtcggggtcg ccgctttccc
    17161 cgcgcaggct cgcctcggcg aggtcgacca gttcggccga gccgtggcgc agcaccaggc
    17221 agtcggcggc gaagtcctcg ctcagccggt cgacataagg ctggcgcacc gtcgccgggg
    17281 tgccgagcac gccgatcacc cggcttttcg aaagcgcggc ggccggcttg atcgccggca
    17341 ccgtgccgac caccggcaga tcgagcgccg cccgcaccgc gtcgagcgcg atggtcgagg
    17401 cggtgttgca ggcgatcacg atcagctccg ggtcgaaccg ctcggccgcg cgcccgagca
    17461 gggccggcac ccgcgccgcg atctcggcct cgctcttggt gccgtaggga tagccggcgc
    17521 tgtcggcgag atagacgatc ggcgcgcccc ggatcagcgc ccggatcggc tgcagcaccg
    17581 acaatccgcc catcccggaa tcgaagatca gcagcggctt cggcatccgc atccggttaa
    17641 gcggcgcccg cccgcgcggc aagcggcccg ccgcccgctg atcgagattg ttgcgccgga
    17701 gcccgttatg ataagctcgc gcgcagcggg ggaatgatcg cgtgaacctt ggactttatg
    17761 ccgagccgct gctgtccttg ctgctcggct atctgctggg gtcgatcccg ttcggcctgc
    17821 tgctgactgc ggccgccggc gcgggcgatc tgcgccgcat cggctccggc aatatcggcg
    17881 ccaccaacgt gctgcggacc gggcgcaagg gcctcgccgc tctaaccctc gcgctcgacg
    17941 gggcgaaggg cgcggccgcg gtgctgatcg ccgccgtcct gttcccgggc ctcgagacgc
    18001 tcgccgcgac cgccgcgctg ctcgggcatc tctacccggt ctggctgcgc ttccgcggcg
    18061 gcaagggcgt cgccaccttc ttcggcatca tggccgcgct ccactggccg tcgtcgctgg
    18121 tcttcgcctc cggctggctg atcatgctcg gcctcacccg ctattcgtcg gtcgcgggca
    18181 tgtcggccgc gctgatcgcc ccggccgccg ccgcggtgat gggccgcttc gacctcgcct
    18241 tgctctatct cggcttcggg ctgctcgtct tctggaagca ccgcgccaat ctcgcgcggc
    18301 tgctcgaagg cgcggagccc cgcgtcggcg cgtcccgcga tggctgagcc cgccgcttcc
    18361 gaggaccaga tcgcgcggct gcgcctgatc cgctccgaca atatcggccc gatcacctat
    18421 ttccaattgc tcgcccgctt cggctcggcc ggcgccgcga tcgaggcgat cccggacctc
    18481 gccgcgcgcg gcggggggag ggcgcccagg ctcgccccgc gcgccgcgat cgagcgcgag
    18541 atcgaggcgg tggcgaggct cggcggccgc tatctgttcc tcggccaggg cctctacccg
    18601 cccttgctgg cggagctcga aagcgcgccg ccggcgctga tcgtcaaggg ccgcctcacg
    18661 ctgctcgaaa agccggcggt ggcgatggtc ggcgcgcgca acgcctccgc cgccgcctgc
    18721 cgcttcgccc gcgcgctggc gcagcggctc ggcgaggcgg gcgtcgtcat cgtctccggc
    18781 ctcgcccgcg gcatcgacac ggccgcgcat gatgggtcgc tcgagaccgg caccatcgcc
    18841 gtcatcgccg gcggcatcga catatcctac ccgcccgaga atgaggcgcg gcaggccgcc
    18901 atcgccgaac gcgggctgct cgttgccgaa cagccgcccg gcgttgagcc gcgcgcccgc
    18961 cacttcccct atcgcaaccg catcatcgcc ggcctcgccc atggcaccgt ggtggtggag
    19021 gccgcgccca aatcgggctc gctgatcacc gcccgctacg ccaccgaatt cggccgcgac
    19081 gtaatggcgg tgcccggctc gccgctcgat ccgagggcac aaggctgcaa ccagctgatc
    19141 cgcgaaggcg cgatcctgat ccagaccgcc gaggacgtgc tcgaggcgat cagcccgttc
    19201 aacctccgtc cggtgcgcca gccgccgctc gactatcgct cgcccgcggc cgccgccgat
    19261 gcgagcgagc aggagcggcg caccatcgcc gatctcctca gccccacccc ggtgccggtc
    19321 gacgaggtgg tccgccagtc cggtttcgcg cccgccacgg tgcagacggt gctgctcgaa
    19381 ctagagctgg cgggaaggct ggagcggcat gccggcggca aggttagcct cgccggctga
    19441 ttttccgcgg ccggggcgga cacgccaccc gcgtgcaacc gcaacaggat taaagcggct
    19501 ttaactctgg aaggttagaa gcaaagtcga tgtcggagcc aaggcccagc ccggagcgaa
    19561 atggcgacgc cgcgcggctg cttctggccg ccgcgcgcga gcgcttttcc gtggccgcga
    19621 ccgatctcct cctgcccgaa gcctcgcgcc tcacccaatg gcagcgcatc accgccgccg
    19681 ccttgctggt ccggctggtc cgcaccgccg aggatgcgct gcgcaccagc ctcgccgacc
    19741 aattccgcgg ccatgaggcg ctccacgccg ctttctcctc cgcccatgtc ccgatcgcgc
    19801 tgcctttgct cgagcgcgcg caggtactgc gcgatgccga gctcggcacc gtcctcgtcc
    19861 gccgggtcga ggagcatcgc ttctggaagg agcaggccga cgccgccgcc gaccgttccg
    19921 agctgatgct ggccctggtc cgcgacgagg acgctgccgt cgccagcctt gccatggcgg
    19981 tgacgatcgc gcgcagccgc cgcttcgacc agtttcacga gccggtgatg ggcgagaccg
    20041 agctcccggc cgagatccag caccgcctcg tctggatgat cgccgcggcg cttcgccaat
    20101 atatgatcca gcagcacggc ctgtcgccgg gcgaggccga cgagggcatc gccgccgccg
    20161 cgggcgagct catccgcggc tatgatgaag gcgacagcct cgaagccgcc tgcacccggc
    20221 tcgcgcgccg cctcgaccag accgagcggc tcgacgatgc gacactcgcg cgcttcctcg
    20281 aagaggggca attgccgctg ttcatcgccg ccattggcgt gcgcgccgcg ctcgattatg
    20341 ctgccacctg ggaagttctt tccgatccgc gcgggcgggg gcccgccttg ctgttgcggt
    20401 cggcaggcgt cgggcgggat catgcggcca acatattgct catcctcaac gcgcgcggcc
    20461 gcatgttctc gggaagcgag ggcgatgccg ccgccgccca gctcgatctg ttcgaagcga
    20521 ccgccgaggc ggaggcgcgc caggtgctgc ggctctggca ggtcgatccc ggctatcgcg
    20581 ccgccatcgc ccggctctcg acccgcgccc gcccggcgcc ggatgccgca tgaccgcgct
    20641 cgtgcagcct gaccccgcgg tcattcgcgg ccggctcgac ggcgagggcc gcctcgtcga
    20701 ggccgatccg ccgctcgccc gactgcatca ccgcgccggc ggccgccagg gcggcgcgct
    20761 cgccgttccg caagtcgccg cgctcgcgcg gctcgcccgc cggctcggca tcgtcatctc
    20821 gcgcggcgcg atcgccgccg acggcattcg cgacatcgac ctctgggtcc gcgccgcgcc
    20881 ggtcggcgac ggcgtcgaga tagtgatcga aagctggacc gagcgcccgg cgcgcggccc
    20941 cgccgatgcg cccgaccagg agcgccaggc cgatttcctc cgcgcctcgg ccgactggat
    21001 gtgggaaacc gacgagagcc tgcgcttcac cacgctgtcg cccgccgccg ccgccgcggt
    21061 cggcaagatc ccggccgaat tggtcggcaa gcagctcacc cgcctgttcc gcttccacga
    21121 aggcgaggac ggcgcgcttc cgatcctgaa cgcgctcgcc gagcagcgcc gcttcgacga
    21181 ccaatatgcc gaattgcgct ccggcaatcg cggccgttac cgcctgagcg gggttcccct
    21241 gatcgacggc accggccgct tcgcgggctt ccgcggcgcc gccgccagcc tgccgcgctg
    21301 gctgcccgac gtcgccaacg atccggcgcc gccgccgccc accaaggact cgcccgcctt
    21361 cggcgaacgg ctcgacaagg ctttgcgcga gccgctcgac catatcatcg ccaatgccga
    21421 gacgatcggc gcgcagccgg acggcccgct cacccccgat tatgccggct atgcgcagga
    21481 cattgcggtc gccggccgcc acctgctcgc gctcgtcgac gacctcatcg acctgcaggc
    21541 gatcgagcgc cccggcttcc ggcccgacgc ggagccgatc gacctggccg agatcgcccg
    21601 ccgcgccgcc ggcttgctgt cggtccgcgc ggccgaaaag caggtccgca tcgaccgccc
    21661 ggccgacgac gagagcctgc ccgcgaccgg cgagttccgc cgcgcgctgc agatcttgat
    21721 gaacctactc accaacgcga ttcgctattc gccggacggc gcccaggtct ggatccgcac
    21781 cgagcgcgag ggcgacctcg cctgcgtgat cgtcgccgac cagggcaagg gcatcgccga
    21841 ggccgaccag gcgcggatct tcgacaagtt cgagcgcgtc gacgtgaccg agcccggcgg
    21901 caccgggctc ggcctctata tcgcgcggcg cctcgcccgg ctgatgggcg gcgacatcgc
    21961 cgtcgacagc gcgccggggc agggcgcgcg cttcacgctc accctgccga tcggtccgga
    22021 aagctgaccc tttaggccgc ctccgccgcg cgcggcggcg ccgtcttcca cattgagaac
    22081 agggtgccgc ccgccaggat cgcgaaggtg atgccgagcg agatcgccgg cgggatcttg
    22141 ccgaggccga gcgcgtcggc gacgaagatc ttggagccga tgaacaccag cagcagcgcc
    22201 agcgcatatt tgagatagtg gaagcgcgcg accatcgccg acagcgcgaa atagagcgcg
    22261 cgtagcccca ggatcgcgaa gatgttcgag gtgtagacga tgaacgggtc ggtggtgatg
    22321 gcgaagatcg ctggcaccga atcgaccgcg aacacgacgt cgacgaactc gaccattatg
    22381 agcgccagga acaagggcgt catgtgccag gccagccgcc cggacgcggc gtcgggctgc
    22441 ctcacccaga agcgctcgcc gtgcagcccc tccgtcaccc ggaagcggcg ataggcccat
    22501 ttcagcaccg gcgatccggc gacgtcatat tccctgtcgg ccatcgccca catcttgatg
    22561 ccggtgaaga tcaggaaggc ggcgaagata tagagcagcc agccgaactg ctcgaccaaa
    22621 gcggcgccga cgccgatcat caccgcgcgc agcacgatca cgcccaatat gccccagaac
    22681 aaaacccggt gctggtagag ccgcggcacc gcgaaatagg tgaagatcat cgcgatgacg
    22741 aagacattgt ccatcgcgag gctcttctcg acgacgaagc cggtgagata gttcatgcct
    22801 gccgtctcgc ccatatacca ccacacccag ccgccgaagg cgaggccgag ggcgacatag
    22861 acggcggaca ggccgaggct ttcgccgacg ccgatctcgc gctgcttgcg gtggaggacg
    22921 ccgaggtcga gcgcgagcag ggcggcgaca atgcccatga agccaagcca catccagacg
    22981 ggcttgccga gaaagtcgag agtcaggaat tccatttatc ggatccaatg cgtggctctt
    23041 cgcgaagagc ggttgcggct cgatccgaca tcgcgcccgc cgcagcggcc gccagagggg
    23101 cccggatcca ttcggaaggg aagggggccg cccgcctgac gcggcgggcg gcggtagctc
    23161 aggcccgcgt ggcggcgagg tgcccgtgca ggcggtcctt gacggccagc cgcagcttct
    23221 tcagccgcag caggcggatc cagtccggcg cgcggcgctt cagctcgcgg cggatttcgt
    23281 cgtccagctt atggtggatc accgacagac ggtaagcaaa gggatgcata gcctctctcc
    23341 aattcgatgt tgctcatcga tcgggtgggc acaatgcggg aagaccaggc atccgaacat
    23401 cgcatcgagc taacccgatg cggtccgaca tcgcgggagt tcaggagaac tcccgccaga
    23461 ggggcccggt ccgcaagata atattatatc gtccgcgcgg cgggatcaag ggcctgcgcg
    23521 cagcatctcc gcggccgccg cctggacctg cagccggata tcgggcaccg ccacgatctc
    23581 ccagaagcac cccttggtga tcggcccgac cgcgaacaga ttgccgcgca ccgagccgtc
    23641 ggccgcgatc acccgcgaat tttcgtccac ttcgaagccg agccgcagcg ggtcgggccg
    23701 cgccatcccg ctcgccagca attgcgcgac cagcgcgtcg tcggcgccgg caatgtcgcc
    23761 cgaaggcccg gtgcagttga tcaccgccgc caccttgcgc accgcctcgc cgccgccgcc
    23821 gcgccgccgg atcgtcaccg ccatcgcgtc cgcctcccgc gccacgcccg cgacgcggcc
    23881 gggcacgatc tcgagccggc cctcggccct cagccgctcc agccgttccg cgacggccgg
    23941 cgcgatgcgg tggcggtgga cgtcccaata aggccgcaag tgccgcagga agcgcttg
//
LOCUS       DASVRK010000290        24138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_603932, whole genome shotgun sequence.
ACCESSION   DASVRK010000290 DASVRK010000000
VERSION     DASVRK010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 24138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24138
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603932"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF589_12015"
     CDS             <1..212
                     /locus_tag="VF589_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258348.1"
                     /translation="AYFQRRKAEISDQESGGLLRRSDEDDLLQRTLDDDAVTDGGEVQ
                     PPPQNEPAPPPEPEPQPEQVIESDD"
     gene            336..605
                     /locus_tag="VF589_12020"
     CDS             336..605
                     /locus_tag="VF589_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258349.1"
                     /translation="MNKIALTIAAVASLGLAACQGSDTAATNETGTDNTEMGATTNEA
                     VTDVNAATTDAMNAADAELDNATQSVENASEAVANAADAATTNTQ"
     gene            711..908
                     /locus_tag="VF589_12025"
     CDS             711..908
                     /locus_tag="VF589_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258350.1"
                     /translation="MRIGLALAALALLGACGEEQDTGGLTAEESRELDEAANMLDTAP
                     AVEGEEALVGEAETAAAGNAQ"
     gene            complement(1205..2854)
                     /gene="groL"
                     /locus_tag="VF589_12030"
     CDS             complement(1205..2854)
                     /gene="groL"
                     /locus_tag="VF589_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049566.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX8258351.1"
                     /translation="MAAKDVRFSRDARERIMRGVDILADAVKVTLGPKGRNVVLDKSF
                     GSPRITKDGVTVAKEIELKDKFENMGAQMLREVASKTNDLAGDGTTTATVLAQSIVRE
                     GMKSVAAGMNPMDLKRGIDLAVIKVVEDLKNRSKPVSGTNEIAQVGIISANGDTVVGE
                     KIAEAMDKVGKEGVITVEEAKGLEFELDVVEGMQFDRGYLSPYFITNPEKMQVELSDP
                     YILLHEKKLSNLQAMLPILEAVVQSGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAVLTGGEMISEDLGIKLETVTINMLGQAKRVTIDKDNTTIVD
                     GAGEGTAIKGRVESIRGMIENTNSEYDREKLQERLAKLAGGVAVIKVGGSSEIEVKER
                     KDRVDDALHATRAAVEEGIVPGGGTALLYATKALQDMKGANDDQTRGIDIVRRALQSP
                     IRQIAENAGHDGAVVAGKLIDGEDQTMGFNAQTEVYENLVQAGVIDPTKVVRTALQDA
                     ASVAGLLITTEAAVSEAPEDKAQGGGGMPGGGMGGMGGMDF"
     gene            complement(2960..3247)
                     /gene="groES"
                     /locus_tag="VF589_12035"
     CDS             complement(2960..3247)
                     /gene="groES"
                     /locus_tag="VF589_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX8258352.1"
                     /translation="MNFRPLHDRVLVRRVEAEEKTAGGIIIPDTAKEKPQEGEVVSVG
                     TGTRGEDGTVTPLDVKAGDKILFGKWSGTEVKLDGEDLLIMKESDILGIVG"
     gene            3465..5342
                     /gene="sppA"
                     /locus_tag="VF589_12040"
     CDS             3465..5342
                     /gene="sppA"
                     /locus_tag="VF589_12040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX8258353.1"
                     /translation="MSMRFMGKVWRLLVGVKDGLVLILMLLFFGLLYAALSVDPYPGS
                     ADEGALLLDLGGTIVEQPAEVDPIDALAGSSSLVREYRLRDVVHALETAATDGRVKAV
                     ALDLDVFTGGGQAALTRVGEAIDSVRRANKPVIAYATGYGDETYQLAAHASEIWVNPL
                     GGVLIAGPGGTGLYFKGLLDKLGVTANVYRAGAYKAAVEPFTRTDMSPESRQANQALA
                     GALWETWLDDVRRARPKAQLAAYVGAPGQRIAAAGGDMARAALAAGLVDKLGDRAAFG
                     KRLAEIVGSDEESVPGSFNAIHYDEWINSNPASTTGGRIGVITVAGEIIDGPAGPGTA
                     GAETIVENLETGLRDHDLSALVVRVDSPGGSTFASERIRQAVLDAKATGLPVVISMGS
                     VAASGGYWIATAGDQILAEPSTITGSIGVFGILPSFEGTLQKLGVGADGVKTTPLSGE
                     PDLYRGPSPEADQLIQSSVLSTYRHFLRLVSAARKLPVERVDAIAQGRVWDGGTARQL
                     GLVDRFGSLDDAIAEAAKRAKLDAADAEAVYLEPAPGLLETFLSSAAGGAEATSRDPF
                     GAIALRPQLLLQRALSDAQAILDGPSIQARCLQCPPSTRPLDRAERQSLAGWLAGLIA
                     G"
     gene            complement(5348..5989)
                     /locus_tag="VF589_12045"
     CDS             complement(5348..5989)
                     /locus_tag="VF589_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX8258354.1"
                     /translation="MAGPAVAAIGPLLEYLDHAGIAVFALSGALVAAAKRQTLVTFIF
                     FAVVTGVGGGTVRDLLIGAPVFWIERNAILVICFAAAMIVWLLPQRLWSGAALLWIDA
                     LGLAAYATFGAAKGLAYGVAPLPAVAMGVLTACLGGIIRDILAAEPSILLRPELYVTA
                     AALSSALMVGLLMMGLDSYAAGAIAASAGFALRAGAIVKGWSLPAYREGGREA"
     gene            complement(6012..6653)
                     /locus_tag="VF589_12050"
     CDS             complement(6012..6653)
                     /locus_tag="VF589_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258355.1"
                     /translation="MFRPSAAAAGAALLLASCGPDTLALPEEMVDRAATCGVVAAAEA
                     RAATPDIKQSLPFTAQGRIVHYAMLAGAQGEEFAADTASAVSKRMSDLQEQITEGKWQ
                     ELAPACRQAFPQAEIAEPKLPGERFEAQLACDEVGDFLSSALSSQESEYGNQLAEYSK
                     LASKLDDTLGPGLRARAGGDLEAQQKVRREALAGAAKLGSPMGLMRQCVERYG"
     gene            complement(6765..8162)
                     /gene="lpdA"
                     /locus_tag="VF589_12055"
     CDS             complement(6765..8162)
                     /gene="lpdA"
                     /locus_tag="VF589_12055"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8258356.1"
                     /translation="MAETEFDVIVIGAGPGGYVAAIRAAQLGLKTCCVESRETLGGTC
                     LNVGCIPSKALLHASELFEEAAHGTMAKWGIKAKEVELDLDVMHAGRREAIKGLTGGV
                     EFLFRKNKVTWLKGHGRFTSASTIDVGGTEYRAKNIVIATGSSVRPLPGVEIDQERIV
                     DSTGALELPQVPKHLVVIGGGVIGLELGSVWRRLGAQVTVVEYLDQILPGMDGEVRKE
                     AAKIFKKQGFALRTSTKVTGAERTAEGVRLTVEPAQGGAAETIEADVVLVSIGRRPNV
                     DGLALDAAGLTLNNRGQIAVDHDFRTGVAGVYAIGDVTPGPMLAHKAEDEGIAVAEIL
                     AGQIGIVNHEVIPSVVYTHPEIAGVGRTEEELKAEGIEVKVGKFPFMANSRAKTNRDT
                     DGFVKVIADARSDRVLGVHIIASLAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEA
                     LKEAAMAVTGLPIHI"
     gene            complement(8149..8520)
                     /locus_tag="VF589_12060"
     CDS             complement(8149..8520)
                     /locus_tag="VF589_12060"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX8258357.1"
                     /translation="MKFLADENLHPLVVQHLRSLGFEVEWIKEAGPGALDEEILSRPD
                     IAQLVLVTLDRDFGDLIFSRGFPAPRAILYSRLSRANPQAIAERIADLVERGLAEHHI
                     TTITALGERVKAFPGVENGRD"
     gene            complement(8524..8760)
                     /locus_tag="VF589_12065"
     CDS             complement(8524..8760)
                     /locus_tag="VF589_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX8258358.1"
                     /translation="MNWRDHIHSDPDILGGKPVVRGTRISVELILEYFADGCSLADVL
                     EAYNHLTPEDVRAALSFAQEMIAEEQALAQKRAA"
     gene            complement(8760..10070)
                     /gene="odhB"
                     /locus_tag="VF589_12070"
     CDS             complement(8760..10070)
                     /gene="odhB"
                     /locus_tag="VF589_12070"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX8258359.1"
                     /translation="MATDVLVPALGESITEATLGQWLKKLGDPVKADEPVASLETDKV
                     AVEVPSPVAGVFDEQVVAEGETVAVGAVIARIGAGTGMPAAAGSQAPAPVSTPVNPAG
                     AGENVELRADEDDGDAEGSNLTLSPAVRRVVLEHGLDPSKIKGTGKDGRLTKDDVLAA
                     AKQATAEGADKAPPAAEAPQAAPPAPAPAAPAAPAFVAPAPAGERREERVRMTRLRQT
                     IARRLKEAQNNAAILTTFNDVDMTEVMAARAKYKDLFEKKHQIRLGFMGFFVKAAALA
                     ARDVPSVNAYIEGDEIVYHDYLDVSVAVSAPNGLVVPVIRNADRLSFADIEKTIADFG
                     RRAKAGTLSMEDMQGGTFTISNGGVFGSLLSTPIINPPQSAVLGMHRIEERPVVREGQ
                     IVARPMMYLALSYDHRLVDGREAVTFLVRMKEAIEDPTRLLIDL"
     gene            complement(10081..12876)
                     /locus_tag="VF589_12075"
     CDS             complement(10081..12876)
                     /locus_tag="VF589_12075"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX8258360.1"
                     /translation="MDRFGEHEQGPSWARDNWPVSELDEVNAGLDPLSMSIEAVAAKA
                     KEVATAAGKTDAEIKQAANDSICAMMLIRTYRVRGHLAADLDPLGLVKREMPTELTPE
                     YHGFSGAALDRTIYLGGALGLEQGTVREVVDILRANYCGHVGLEYMHINDLDERRFLQ
                     DRMEGREAAIRFTPEGKQSILTKVIEGEQWERFLARKYVGTKRFGLDGGEAMIPALEA
                     VIKYGGQYGVRDIIFGMAHRGRLNVLANVMAKPYRAIFSEFAGGSANPEDVGGSGDVK
                     YHLGTSSDREFDGIRVHLSLLPNPSHLEAVNPVVLGKARAQQVMRGDEDGSEVLPVLL
                     HGDAAYAGQGIVAEILALSGLPGYGTGGTLHFIINNQVGFTTSPQFARSSPYPSDVAK
                     MVQAPILHVNGDDPEAVTFACKLAIDYRQTFKRDVVIDMWCYRRFGHNEGDEPSFTQP
                     QMYAAIRNHPPISDIYGARLVQEGVVDRAWIDAAIAGFTAVLENEFEAAKSYLPNKAD
                     WFEGRWTGLGRPDTPESGRRNAPTGLDEETIRRVGKILTTIPENLTPHKTLTRIVDAR
                     RQMFESGEGFDWATAEALAFGSLVKDGFQVRLSGQDSGRGTFSQRHSVWVDQNDGRKY
                     IPLCQIEGGRFEVRDSPLSEMGVLGFEYGYALSDPHTLVLWEAQFGDFANGAQVIIDQ
                     FIASGEAKWLRANGLVMLLPHGYEGQGPEHSSARPERYLQLCAEDNMQVANCTTPANY
                     FHILRRQMHRDFRKPLVIMTPKSLLRHKLAVSSLADFSGDSHFKRILSDPRPPADGEV
                     KRLVLCSGKLAYELIEARDKAEARDTCVVRIEQLYPFPSEPLIERLKRMTALEELVWA
                     QEEPRNNGYWNFAEPFIEQCLAEAGAKPQRARYAGRASSASPATGLAKRHAAEQAALI
                     ADALGHSSDARLQRKAG"
     gene            complement(12935..13819)
                     /gene="sucD"
                     /locus_tag="VF589_12080"
     CDS             complement(12935..13819)
                     /gene="sucD"
                     /locus_tag="VF589_12080"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX8258361.1"
                     /translation="MSILVNATTKVITQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GQTHIGLPVFDTVHEAVEATGADASVIYVPPAFAADSILEAIDAEVPLIVAITEGIPV
                     LDMVKVKRALTGSKSRLIGPNCPGVLTPGECKIGIMPGNIFRKGSVGVVSRSGTLTYE
                     AVFQTSNAGLGQTTAVGIGGDPVNGTNFIDVLEMFLADDETQSIIMIGEIGGSAEEDA
                     AQFVADEARRGRAKPMVGFIAGRTAPPGRRMGHAGAIVAGGKGGADDKIAAMEAAGIR
                     VSPSPSELGTTLSELLKG"
     gene            complement(14298..14633)
                     /locus_tag="VF589_12085"
     CDS             complement(14298..14633)
                     /locus_tag="VF589_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258362.1"
                     /translation="MTLKYQDPQRHGIEDQCILASRFVAPSRAIGRLTGLLSGGRIRN
                     PASSKTVAWQMDAPQHRYVMRVWRTHPYTVMSTTVLDEAEAARRAEAQRKYWDRKRSK
                     RRPGRERAE"
     gene            complement(14702..15190)
                     /locus_tag="VF589_12090"
     CDS             complement(14702..15190)
                     /locus_tag="VF589_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258363.1"
                     /translation="MDVFIAACLRGEAKLKPEQSISVTLEHLPKQLRREYRPAGGTFV
                     KLAGSASSFLYWFDQSPHPRRFARVCGIASQIVDIGFAWRRISLEIDRVSYEGPAAEH
                     VGNPTQLMFERPEHGYTLSYASGVGGYSVVQIGWMTEQQRESSLIRRRRVDKLIENKK
                     KK"
     gene            complement(15285..15392)
                     /locus_tag="VF589_12095"
                     /pseudo
     CDS             complement(15285..15392)
                     /locus_tag="VF589_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     gene            complement(15389..15952)
                     /locus_tag="VF589_12100"
     CDS             complement(15389..15952)
                     /locus_tag="VF589_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258364.1"
                     /translation="MTSLLLALAGLAGLGADYPAAPILAEFDKACAVSASHGRQEEAI
                     VAGGWTVHKSVSGGRLHSLVGAMTSQSASLRFKTYQKAVAGRTLHLLLTDSSTTDGAG
                     RLSCVVYDFDAQSGVDPAQAREWAGRPAEMAEKSSGRSVMAWAPGLRPGHASDTIIFF
                     APGAAKFGGVDFSGLVLSAKYKESGAE"
     gene            complement(15949..16911)
                     /gene="mdh"
                     /locus_tag="VF589_12105"
     CDS             complement(15949..16911)
                     /gene="mdh"
                     /locus_tag="VF589_12105"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX8258365.1"
                     /translation="MARKKIALIGAGMIGGTLAHLAAIREMGDIILFDVAEGVPQGKA
                     LDLAQAGPVEGYDVELRGSNDYADIAGANLCIVTAGVPRKPGMSRDDLLGINLKVMKA
                     VGEGIRAHAPTAFVICITNPLDAMVWALREFSGLPHHMVVGMAGVLDSARFRHFLAEE
                     FNVSVEDVTAFVLGGHGDTMVPVPAYSTVGGIPIPDLIKMGWSSQEKIDAIVQRTRGG
                     GGEIVGLLKTGSAFYAPATSAIMMAESFLKDKKRILPAAANLTGQYGVNDLYVGVPVV
                     IGAGGVERIVEIELDETARQNFDVSVDAVKELLVACKGIDESLA"
     gene            complement(17023..18132)
                     /gene="zapE"
                     /locus_tag="VF589_12110"
     CDS             complement(17023..18132)
                     /gene="zapE"
                     /locus_tag="VF589_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX8258366.1"
                     /translation="MGPVGGCYERLLTAGELRPDEDQRRAVEHLDRLAAELEAKPKRG
                     LLAKLAGKAPKVVRGLYLWGGVGRGKSMLMDLAYEAIAFAPKRRVHFHEFMLEVHERL
                     RAERAKEEGDPIPPVAEAIAAEARLLAFDEMVINNSADAMILSRLFGRLLEEGVTVIT
                     TSNRPPGDLYKNGLNRELFLPFIDLVERELEVVPLNGPTDYRLERLGGLPTWHVPNGP
                     EATRALSEAFFRLTDYAVENRDKVPSEDIPVQGGRTLHVPKSLKGVVVFSFRRLCGEA
                     RGAPDYLAIARRYHTVILVGIPKLGPENRNEAARFVTLIDALYEHKVKLLAAADATPA
                     DLYTAGDGAFEFERTASRLIEMQSEDYLARGHGEE"
     gene            complement(18606..19391)
                     /locus_tag="VF589_12115"
     CDS             complement(18606..19391)
                     /locus_tag="VF589_12115"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX8258367.1"
                     /translation="MAEFALPKNSKIKGKGRVHKAAGGPGRYKRVRVYRYDPESGENP
                     RYDTFEINLDETGPMVLDALIKIKNEIDPTLTFRRSCREGICGSCSMNIDGRNGLACT
                     TAIEDVKGEVRITPLPHMDVIKDLVPDFTHFFAQYASIEPWLKTVTPTPSDKERLQSP
                     DDRAKLDGLYECIWCACCSSSCPSYWWNSDRFLGPAILLQAYRWLADSRDERTGERLD
                     ELEDPFRLYRCHTIMNCANVCPKGLNPAKAIAETKKLLLERAI"
     gene            complement(19473..20516)
                     /locus_tag="VF589_12120"
     CDS             complement(19473..20516)
                     /locus_tag="VF589_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase"
                     /protein_id="HEX8258368.1"
                     /translation="MNGSARPLAIPRSARESLRIMFIAKHANWRGGLHPEDGSHAIYH
                     QEIRRILEELGLNLILADSYRSLFEHPGADFVFPLLNRGGFLNSEMLLPLLCNRLGIP
                     FLGASPILRGVSDDKHLTKLAAAARGIPTAPWAIYRRGTAIDTAHCPRAQRYVVKPNA
                     SSASWGVSDAHDLDGVRRAVAELHEQGHDAIVEPFITGSDIEVSVITVDGEPLILPTM
                     IVEQSDPELLRTYNEKRNLAGGQRYEILPFRDARWLPVIEEHARNIMREFLPFDYGRF
                     EFRMDYKSGQVQFLEVNLNCNLWSEKTISKAARLAGWSHAELIETILCESLRRNDLLG
                     KRAARPERYARVA"
     gene            complement(20641..21714)
                     /locus_tag="VF589_12125"
     CDS             complement(20641..21714)
                     /locus_tag="VF589_12125"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HEX8258369.1"
                     /translation="MVWLALRRWGPRRWSDVRLCLREGLVPRDLDEARENIGPGAEQS
                     WVSPRAKRAICALLDPEGAGDDRALSDKCHFDRLARAAGLPLPETIGRGALDAPLPAW
                     IESGSGLILKRGYSLQGKGIAALAREGAEWRADGGPVVDLIGWLRANLDPAGNVQRRL
                     RTHAALADLSPTALPTLRVIVCLDERGEPEPTHAFLRLGPIGSALDNFSAGGISAAAN
                     LGTGILAEARIRHGRGGRVDRISRHPVTDAAIEGREVPLFQAAVALAAEAHRRLFGGR
                     RVLIGWDIGLSEDGAMLIEGNWNPGSPLLQLVSGTGIAGHRLGDLMTFHLRRLQPSVF
                     ASMPVVMRMRGPPAPPAPALHLQ"
     gene            complement(21945..22913)
                     /locus_tag="VF589_12130"
     CDS             complement(21945..22913)
                     /locus_tag="VF589_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8258370.1"
                     /translation="MIVRLAIAYLTALAPAPAAVPIGDGVHFIQGGFGAGRQPDGNSV
                     VFEAPDGLIVVDSGRHAEHQRKILALAAARRMPIAALVNTHWHLDHSGGNAELREAAG
                     GELSIHASRAVEGALTGFLARSKAEAVAFLASGKADAATAAEIEGDIAAVDERRSLLP
                     TAPVDGPGERRIAGRPLSLRLAQHAATAGDVWVYDAASGIVAVGDLVTLPVPFFDTAC
                     PEGWRRALTEIAGTPFTRLVPGHGAVMGRPEFMVWKRAFERLHDCAGSSRGDGACIAG
                     WQSDAAALLAPDEGRQADAMLGYYLKTRLRGKPGERVRYCPGGADG"
     gene            complement(22997..23431)
                     /locus_tag="VF589_12135"
     CDS             complement(22997..23431)
                     /locus_tag="VF589_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8258371.1"
                     /translation="MDGAPPIEWRVHVRSSPEAAYEAWTKDAGRSRFWAEESRSGPGG
                     FELRFVNGQALAVEVVEAMPATRLVFRYFGGSTVTLAFEPDGRGGCDLYLREEGVPAE
                     EHLENYAGWVSVLLAFKAAVDFGIDLRAHDPKRTWERRHVDC"
     gene            complement(23431..>24138)
                     /locus_tag="VF589_12140"
     CDS             complement(23431..>24138)
                     /locus_tag="VF589_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX8258372.1"
                     /translation="GIPTNYWGEVVTLKDGNPDATVPGLYAVGEAACVSVHGANRLGS
                     NSLIDLVVFGRATGHRLGELLKPGAAQRPLPRGSEEMPLSRLDGFRHAKGPTPTAEIR
                     AAMQKVMQADCAVFRTEATLAEGKAKIDEVQRRMGDIRVTDRSLVWNTDLIETLELDN
                     MLPQAVVTMHSAANRKESRGAHMHEDYPNRDDANWMKHTIAWFGQGSAVTIDYRPVHD
                     YTLTDEIQYIKPKARVY"
BASE COUNT         3801 a         8301 c         7872 g         4164 t
ORIGIN      
        1 cggcctattt ccagcgccgc aaggcggaga tcagcgacca ggaaagcggc ggcctgctgc
       61 gccgttcgga cgaggacgac ctcctccagc gcaccctcga cgacgatgcc gtgaccgacg
      121 gcggcgaggt ccagccgccg ccgcaaaacg agcccgcgcc gccccccgag cccgagccgc
      181 aacccgagca agtgattgaa tcggatgatt gactactaaa atagtagaca tgtctccccc
      241 cctcctgtag atcgcgcagt cgccggatcg gtgggggatg ctcccctgtc gcctcgcccg
      301 gcgaaccaag tgagtcccca aggagggtat tgcccgtgaa caaaattgct ctgaccatcg
      361 ccgccgtggc gtcgctcggc ctcgccgctt gccagggtag cgacaccgct gccaccaacg
      421 agaccggcac cgacaacacc gaaatgggcg ccaccaccaa cgaagcggtg accgacgtca
      481 atgccgccac gacggacgcg atgaacgcgg ccgacgcgga gctcgacaat gccacccagt
      541 ccgtcgagaa cgcctcggaa gcggttgcca acgccgccga cgccgcgacg accaacaccc
      601 agtaagcttc gcgcttgctg aacggatcgg ggccgggcgc tagcgtccgg cccctttctt
      661 ttgcccgcga tccggctagg ctccgggcgc ttcaccaggg gagttttagg atgaggatcg
      721 gtttggcact ggcggcgctg gctttgctcg gcgcgtgcgg cgaggagcag gatacgggcg
      781 gcctcaccgc cgaggaatcg cgcgagctcg acgaggccgc caacatgctc gacaccgcgc
      841 ccgccgtgga gggcgaggaa gccctggtcg gcgaagccga gaccgcggcc gcgggcaacg
      901 cgcagtaagg cggcgcttcg aaggtcgggg gcggcgacga agcctcggaa ggccccccgc
      961 cccggcacct gcatcatttc gagatagaga gatatcgtcc tgccggactt gatccggcat
     1021 ctcccttcct tccctcctac gccgggcgac ctgacgaaga agggggaccc cggatcaagt
     1081 ccagggtgac gaccacccct atcatcgagc gcctcgtacc agcgccactc accgacgccc
     1141 ccaacaagaa aaaggccggg agtctcctcc cggcccttct tcttttgcgt cgggtgcgtg
     1201 gaccttagaa gtccatgccg cccatgccgc ccatgccgcc gccgggcatg ccgccgccgc
     1261 cctgggcctt gtcttccgga gcttcgctca ccgccgcttc ggtcgtgatg agcaggcccg
     1321 cgaccgacgc ggcgtcctgg agcgcggtgc ggacgacctt ggtcgggtcg atcacgccgg
     1381 cctggaccag gttctcgtag acctcggtct gggcgttgaa gcccatcgtc tggtcctcgc
     1441 catcgatcag cttgccggcg accacggcgc cgtcatggcc cgcattctcg gcgatctggc
     1501 ggatcggcga ctgcagagcg cggcggacga tgtcgatgcc gcgggtctgg tcgtcattgg
     1561 cgcccttcat gtcctgcagc gccttggtgg cgtagagcag agcggtgccg ccgccgggga
     1621 cgatgccttc ctcgaccgcg gcgcgggtgg cgtggagggc gtcatcgacg cggtccttgc
     1681 gctccttgac ctcgatctcg gacgaaccgc cgaccttgat cacggcaacg ccgccggcga
     1741 gcttggcgag gcgctcctgg agcttctcgc ggtcatactc gctgttggta ttctcgatca
     1801 tgccgcggat cgattcgacc cggcccttga tggctgtgcc ctcgcccgcg ccgtcgacga
     1861 tggtcgtgtt gtccttgtcg atggtgacgc gcttggcctg gccgagcatg ttgatcgtga
     1921 cggtctcgag cttgatgccg agatcttccg agatcatctc gccgccggtc agcaccgcga
     1981 tgtcctcgag catcgccttg cggcgatcgc cgaagcccgg cgccttgacg gccgcgacct
     2041 tgagtccgcc gcgcagcttg ttgacgacca gcgtcgccag agcttcgccc tcgatatcct
     2101 cggcgatgat caggagcgga cggcccgact ggaccaccgc ttccaggatc gggagcatcg
     2161 cctgcaggtt cgacagcttc ttctcgtgga ggaggatgta ggggtcgcta agctcgacct
     2221 gcatcttctc cgggttggtg atgaagtaag gcgagagata gccgcggtcg aactgcatgc
     2281 cttcgacaac gtcgagctcg aactcgagcc ccttggcctc ctcgaccgtg atcacgcctt
     2341 ccttgccgac cttgtccatc gcctcggcga tcttctcgcc gacgacggtg tcgccattgg
     2401 ccgagatgat gccgacctgg gcgatctcat tggtgccgct gaccggcttg gaccggttct
     2461 tgagatcctc gacgaccttg atgacggcga ggtcgatgcc gcgcttcagg tccatcgggt
     2521 tcatgcccgc cgcgaccgac ttcatgcctt cgcggacgat cgactgggcc agaacggtgg
     2581 cggtggtggt gccgtcgccg gccaggtcat tggtcttcga ggccacttca cgcagcatct
     2641 gcgcgcccat attctcgaac ttgtccttga gctcgatctc cttggcgacg gtgacgccgt
     2701 ccttggtgat gcgcgggctg ccgaagctct tgtcgaggac gacgttgcgg cccttggggc
     2761 cgagcgtgac cttcaccgcg tcggcgagga tgtcgacgcc gcgcatgatg cgctcccggg
     2821 cgtcgcgcga aaagcgtacg tctttcgctg ccatgtttct atgttccttc taatttctgt
     2881 tctcctgcga aagcaggagt ctagcccaac aaagagtctg ggctcctgcc ttcgcaggag
     2941 cacatccttt tcgaaaaggt cagccgacga tcccgaggat gtcgctttcc ttcatgatca
     3001 gcaggtcctc gccgtcgagc ttgacctcgg tgccggacca tttgccgaac aggatcttgt
     3061 cgccggcctt gacgtcgagc ggagtgaccg tgccgtcctc gccgcgcgtt cccgtgccga
     3121 ccgagacgac ttcgccttcc tgcggcttct ccttggccgt atcggggatg atgatgccgc
     3181 cggcggtctt ctcttcggcc tcgacgcggc gcaccagtac ccggtcatgg agcggacgaa
     3241 aattcatgcg cgttcctttc ttctaatcgg ccgcgacaag ccccccgctt cttcgggaac
     3301 atgtcgctgg cctggagttt gttagcactc gatcctgccg agtgccagcg agccggatgt
     3361 aggccttgtt gatgtgaggt caagcgcgga ggcgaagatt ttcggcgttc cggttgtcag
     3421 cgggggccag ccgtcgcatc tagggcgttg aacgtcgagc ggggatgagc atgcgcttca
     3481 tgggtaaggt ctggcggttg ctggtcggcg tcaaggatgg gctcgtgctc atcctcatgc
     3541 tcctgttctt cggcttgctc tacgcggccc tctcggtcga tccctatccg ggaagcgcgg
     3601 acgaaggcgc gctgctgctc gaccttggcg gcacgatcgt cgagcagccc gccgaagtcg
     3661 atccgatcga cgcgctcgcc ggcagctcct ctttggtccg cgaataccgg ctgcgcgacg
     3721 tcgtccacgc gctcgagacc gcggccacgg acgggcgggt caaggcggtc gcgctcgatc
     3781 tcgacgtgtt caccggcggc ggccaggcgg cgcttacccg ggtcggcgag gcgatcgaca
     3841 gcgtccgtcg cgccaacaag ccggtgatcg cctacgccac cggctatggc gacgagacct
     3901 accagcttgc cgcccacgcg tccgaaatct gggtcaaccc gctcggcggc gtgctgatcg
     3961 ccgggccggg cggaaccggc ctctatttca agggcctgct cgacaagctc ggggtgaccg
     4021 ccaacgtcta tcgcgccggc gcctacaagg cggcggtcga gcccttcacc cgcaccgaca
     4081 tgtcgcccga atcgcgccag gccaatcagg cgctggcggg ggccttgtgg gagacctggc
     4141 tggacgacgt ccgccgcgcc cgccccaagg cgcagctcgc cgcttatgtc ggcgcgcccg
     4201 ggcagcggat cgcggccgcg ggcggcgaca tggcgcgcgc cgcgctcgcc gccggccttg
     4261 tggacaagct cggcgaccgc gccgccttcg gcaaaaggct ggcggagatc gtcggctccg
     4321 acgaggagtc ggtgcccggc agcttcaacg ccatccatta tgacgaatgg atcaactcca
     4381 acccggccag cacgaccggc ggccggatcg gcgtcatcac cgtcgccggt gagatcatcg
     4441 acgggccggc cgggcccggc accgccggcg ccgagacgat cgtcgagaat ctcgagaccg
     4501 ggcttcgcga ccatgacctc agcgctttgg tcgtcagggt cgattcgccg ggcggctcga
     4561 ccttcgcctc ggagcgcatc cgccaggcgg tgctcgacgc caaggccacc ggccttccgg
     4621 tcgtcatctc gatggggtcg gtcgcggcca gcggcggcta ttggatcgcc accgccggcg
     4681 accagatttt ggccgaaccc tccaccatca ccggctcgat cggggtgttc ggcatcctgc
     4741 cgagcttcga gggcacgctg caaaagctcg gcgtcggcgc cgacggcgtc aagaccaccc
     4801 cgctgtcggg cgagcccgat ctctatcgcg gcccctcgcc cgaggccgac cagctgatcc
     4861 agagcagcgt cctcagcacc tatcgccact tcctccgcct ggtctcggcg gcgcgcaagc
     4921 tgccggtcga gcgggtcgac gcgatcgcgc agggccgcgt ctgggacggc ggcaccgccc
     4981 gccagctcgg cctggtcgac cggttcggct cgctcgacga cgccatcgcc gaagcggcca
     5041 agcgcgccaa gctcgacgcg gccgatgccg aggcggtcta tctcgagccc gcgcccggcc
     5101 tgctcgaaac cttcctgagc agcgccgccg gcggggccga ggccacgtcg cgcgacccgt
     5161 tcggcgccat tgcgttgcgg ccccaattgc tgctgcagcg cgcgctgagc gacgcccagg
     5221 cgatcctcga cggcccgtcg atccaggccc gctgcctgca atgcccgccg agcacccgcc
     5281 cgctcgaccg ggccgagcgc cagtcgctgg ccggctggct ggccggccta atcgccggct
     5341 agcgcaccta ggcttcgcgc ccgccctcgc gataggcagg aaggctccag cccttgacga
     5401 tggcccccgc cctgagcgcg aagccggccg aggccgcgat cgcgccggcc gcatagctgt
     5461 cgaggcccat catcaacagc ccgaccatca gcgccgagga caaggccgcg gcggtgacgt
     5521 agagctcggg ccgcagcagg atcgacggct cggcggcgag aatgtcgcgg atgatgccgc
     5581 cgaggcaggc ggtcagcacg cccatcgcta ccgccggcag cggcgcgacc ccgtaagcca
     5641 gccccttggc ggcgccgaag gtggcgtagg cggcaagccc gagcgcgtcg atccacagca
     5701 aggcggcccc ggaccatagc cgctgcggga gcagccagac aatcatcgcc gccgcgaagc
     5761 agatgacgag gatcgcattg cgctcgatcc agaagaccgg cgccccgatt aaaaggtcgc
     5821 gcacggtgcc cccgccgacg ccggtcacga ccgcgaagaa gatgaaggtc accaaagtct
     5881 ggcgcttggc ggccgcgacc agcgcccccg acaaggcgaa aacggcgatg ccggcatggt
     5941 cgagatattc gagcagcggg ccgatcgcgg cgacggccgg cccggccatc agcccgcccg
     6001 cccggtcgcg ctcacccgta gcgttcgacg cactggcgca tcagccccat cgggctgccg
     6061 agcttggcgg cgccggcgag cgcctcgcgc cgcacctttt gctgcgcctc gaggtcgccg
     6121 cccgcgcgcg cgcgcagccc tggcccgagc gtatcgtcga gcttgctcgc cagcttgcta
     6181 tattcggcga gttggttgcc atattccgac tcctggctcg acagcgccga agacaggaaa
     6241 tccccgacct cgtcgcacgc cagctgcgcc tcgaaccttt cccccggcag cttcggctcg
     6301 gcaatctccg cctgcgggaa agcctggcgg caggcggggg cgagctcctg ccatttgcct
     6361 tcggtgatct gctcctggag gtccgacatg cgcttgctga ccgcgcttgc cgtgtcggcc
     6421 gcgaattcct cgccctgcgc gcccgccagc atcgcataat gaacgatccg cccctgagcg
     6481 gtgaacggca gcgactgctt gatgtcgggc gtggcggccc gggcctcggc cgcggcgacc
     6541 acgccgcagg tggcggcgcg gtccaccatc tcctcgggca gcgccagcgt gtcgggtccg
     6601 caggacgcca gcaaaagcgc ggcgcccgcg gcagcggcgg aaggtcggaa catgtgggcc
     6661 ctctcgttga ccgttacgga accacaacgg acaggccggg cgcgggttcc tgcccttggc
     6721 agccgaggaa ttcccgatgc ccgccggttc ccgcgccttc aggatcagat atggatcggc
     6781 agccccgtca ccgccattgc cgcttccttc agcgcctccg aatgggtcgg gtgggcgtgg
     6841 caggtgtaag cgatatcctc cgaggtcgcc ccgaactcca tcgcctgggc cgcctgcgcg
     6901 atcatcgtgc cggcgagcga ggcgatgata tggacgccga gcacccggtc ggaacgcgcg
     6961 tcggcgataa ccttgacgaa gccgtccgtg tcgcgattgg tcttggcgcg gctgttcgcc
     7021 atgaagggga atttgccgac cttcacttcg atgccctcgg ctttaagctc ctcttcggtg
     7081 cggccgacgc cagcgatctc gggatgcgtg tagacgaccg acgggatcac ctcgtggttg
     7141 acgatgccga tctggccggc gaggatctcg gcgacggcga tgccttcatc ctccgccttg
     7201 tgggcgagca tcggtcccgg cgtcacgtcg ccgatcgcat acacgccggc gacgccggtg
     7261 cggaaatcgt ggtcgaccgc gatctgcccg cgattgttga gcgtcagccc ggccgcgtcg
     7321 agcgcgagcc cgtcgacatt gggccggcgg ccgatcgaaa ccagaaccac gtccgcctcg
     7381 atcgtctcgg ccgcgccgcc ctgggccggc tcgacggtca ggcgcacgcc ctcggccgtc
     7441 cgctcggcgc cggtgacctt ggtcgaggtg cgcagcgcga agccttgctt cttgaagatc
     7501 ttggccgctt ccttgcggac ctcgccgtcc atcccaggca ggatctggtc gagatattcg
     7561 accaccgtca cctgcgcgcc gagccgccgc cagaccgagc cgagctccag cccgatcacg
     7621 ccgccgccga tcacgaccag gtgcttcggc acctgcggca attcgagcgc gccggtcgaa
     7681 tcgacgatcc gctcctggtc gatctcgacc ccgggcagcg gcctcaccga cgatccggtc
     7741 gcgatgacga tattcttggc gcgatattcg gtgccgccga cgtcgatcgt gctcgcggag
     7801 gtgaagcggc catggccctt gagccacgtc accttgttct tcctgaacag gaactcgacg
     7861 ccgccggtca gccccttgat cgcctcgcgc cggccggcat gcatgacgtc gaggtcgagc
     7921 tcgacctcct tggccttgat cccccatttg gccatcgtgc cgtgcgccgc ttcctcgaac
     7981 agctcggagg cgtgcagcag cgccttggac gggatgcagc cgacgttgag gcaggtcccg
     8041 cccagcgtct cccggctttc gacgcagcac gtcttgagcc ccagctgcgc cgcccggatc
     8101 gcggcgacat agccgcccgg cccggccccg atcacgatca cgtcgaattc agtctctgcc
     8161 attctcaact ccagggaatg ccttcacccg ctcgccgaga gcagtgatgg tcgtgatatg
     8221 gtgctcggcc aggccgcgct caaccagatc ggctatccgc tccgcgatgg cctgcggatt
     8281 cgccctgctg agccggctgt agaggattgc gcggggagcc ggaaaccccc ggctgaagat
     8341 caggtcgcca aagtcgcggt ccagcgtcac cagcaccagt tgcgcgatgt cgggccggga
     8401 gagaatctct tcgtccagcg cgcccggacc ggcctccttg atccattcca cctcgaaccc
     8461 gagcgacctc aaatgctgca cgaccagcgg gtgcagattt tcgtcggcaa ggaacttcaa
     8521 ggttcaggcg gcgcgcttct gggccagcgc ctgctcctcg gcgatcatct cctgcgcgaa
     8581 ggaaagtgcc gcccgcacat cctccggcgt gagatggttg taggcctcca gcacatcggc
     8641 cagactgcag ccgtcggcga aatattccag gatgagctcc accgagatac gcgtgccgcg
     8701 caccaccggc ttcccgccga gaatatcggg atccgaatgg atatggtcgc gccagttcat
     8761 cacaggtcga tcagcagcct cgtcggatcc tctatagcct ctttcatccg caccaggaag
     8821 gtgaccgcct cgcggccgtc gaccaggcgg tggtcgtagg acagcgccag gtacatcatc
     8881 ggccgcgcga cgatctggcc ctcgcgcacc accggccgct cctcgatccg gtgcatgccg
     8941 agcaccgccg actgcggcgg attgatgatc ggggtcgaaa gcaacgagcc gaacacaccg
     9001 ccgttcgaga tggtgaaggt gccgccctgc atgtcttcca tggaaagcgt gcccgccttg
     9061 gcgcgcctgc cgaagtcggc gatcgtcttc tcgatgtcgg cgaagctcag ccggtcggcg
     9121 ttgcggatga cgggcacaac gaggccgttg ggcgcgctca ccgccaccga cacgtcgaga
     9181 tagtcgtgat agacgatctc gtcgccctcg atataggcgt tgaccgaggg cacgtcgcgg
     9241 gcggcgagcg ccgccgcctt cacgaagaag cccatgaagc cgagccggat ctggtgcttc
     9301 ttctcgaaca gatccttata tttcgcgcgc gccgccatca cctcggtcat gtcgacgtcg
     9361 ttgaaggtgg tgaggatggc ggcgttgttc tgcgcctcct tcaggcgccg ggcgatggtc
     9421 tggcgcagcc gggtcatgcg cacgcgctcc tcgcggcgct cgccggccgg cgccggcgcg
     9481 acgaaggcgg gcgcggccgg agcggccgga gccggagcgg ggggtgccgc ttgcggggcc
     9541 tcggcggcgg gcggcgcctt gtccgcccct tcggcggtgg cctgtttggc ggcggcgagc
     9601 acgtcgtcct tggtgaggcg gccgtccttg cccgtgccct tgatcttgga aggatcgagc
     9661 ccgtgctcga gcacaacccg gcgaacggcg ggcgacagcg tcaggttgga accctcggca
     9721 tcgccgtcat cctcgtcggc gcggagctcg acattctcgc cggctccggc cgggttcacc
     9781 ggggtcgaga ccggcgccgg cgcctgcgat ccggccgcgg ccggcatgcc cgtgccggcg
     9841 ccgatgcgcg cgatcacggc gccgaccgcg acggtctcgc cttccgccac cacctgctcg
     9901 tcgaacacgc cggcgaccgg cgaggggact tcgaccgcca ccttgtcggt ctccaggctc
     9961 gccaccggct cgtccgcctt gacggggtcg ccaagcttct tgagccactg gccgagcgtc
    10021 gcctcggtga tcgattcccc gagggccgga acgagtacgt cggttgccat atttccccta
    10081 tcagccggct ttgcgctgca ggcgcgcatc gctgctatgc ccaagtgcgt ccgcgatcaa
    10141 tgccgcctgc tcggcggcgt gccgcttggc gagcccggtg gcgggcgatg ccgacgaggc
    10201 gcggccggca tagcgggcgc gctgcggctt cgcgccagcc tcggccaggc attgctcgat
    10261 gaacggctcg gcgaaattcc aatagccgtt gttgcgcggc tcctcctgcg cccagaccag
    10321 ctcctcgagc gcggtcatcc gcttcagccg ctcgatcagc ggctcggacg ggaaggggta
    10381 gagctgctcg atccgcacca cgcaggtgtc gcgcgcctcc gccttgtcgc gcgcttcgat
    10441 caactcgtag gcgagcttgc ccgagcacag gacgagccgc ttgacctcgc cgtcggcggg
    10501 cgggcgcgga tcggacagga tgcgcttgaa atggctgtcg ccgctgaagt cggcgaggct
    10561 cgagacggcc agcttgtgcc gcagcagcga cttgggcgtc atgatcacca gcggcttcct
    10621 gaagtcccga tgcatctggc ggcggaggat gtggaaataa ttggctggcg tggtgcagtt
    10681 ggcgacctgc atattgtcct ccgcgcacag ctggaggtag cgctccgggc gggccgacga
    10741 atgttccggc ccctggcctt catagccgtg cgggagcagc atcacgaggc cgttggcgcg
    10801 cagccatttg gcttcgccgc tggcgatgaa ctggtcgatg atgacctggg cgccgttggc
    10861 gaaatcgccg aactgcgcct cccacagcac cagggtatgc gggtccgaaa gcgcatagcc
    10921 atattcgaag ccgagcacgc ccatttcgga gagcgggctg tcgcgcactt cgaagcggcc
    10981 gccctcgatc tggcagagcg ggatatattt gcggccgtca ttctggtcga cccacaccga
    11041 gtggcgctgg ctgaacgtgc cgcggccgct gtcctggccg gacaggcgca cctggaagcc
    11101 atctttgacc aaagatccga acgccagcgc ctccgccgtc gcccagtcga agccctcgcc
    11161 gctctcgaac atctggcgcc gcgcgtcgac gatccgggtc aatgttttgt gaggtgtcag
    11221 attttcgggg atcgtggtga gaatcttgcc gaccctccgg atggtctcct catcaaggcc
    11281 ggtgggcgcg ttgcggcggc cgctctcggg cgtgtcggga cgtccgagcc cggtccagcg
    11341 gccctcgaac cagtcggcct tgttcggcag atatgatttg gccgcctcga actcgttttc
    11401 cagcaccgct gtgaaccccg cgatcgcggc gtcgatccag gcgcggtcga ccacgccctc
    11461 ctggaccagc cgcgccccgt atatgtcgga aatgggcggg tggttgcgaa tcgcggcata
    11521 catttgcggc tgggtgaagc tcggctcgtc gccctcattg tggccgaagc ggcggtagca
    11581 ccacatgtcg atgacgacgt cgcgcttgaa cgtctggcga tagtcgatgg cgagcttgca
    11641 ggcgaaggtc accgcctcgg gatcgtcgcc gttgacgtgg aggatcggcg cctggaccat
    11701 cttcgcgacg tcggagggat agggtgagga gcgcgcgaat tgcggcgagg tcgtgaagcc
    11761 gacctggttg ttgatgatga aatggagcgt gccgccggtg ccgtagccgg gcagacccga
    11821 cagggcgagg atctcggcga cgatcccctg gccggcataa gcggcgtcgc cgtgaagcag
    11881 caccgggagc acctcgctgc cgtcctcgtc gccgcgcatc acctgctgcg cgcgcgcctt
    11941 tcccagcacc accgggttga ccgcctcgag gtggctcggg ttgggcagca gcgagaggtg
    12001 gacgcgaatg ccgtcaaact cgcggtccga cgaggtgccg agatgatatt tgacgtcgcc
    12061 cgatccgccg acgtcctcgg gattggcgga gccgccggcg aactcgctga agatcgcgcg
    12121 atagggcttg gccatgacgt tggcgagcac gttgagccgg ccgcgatggg ccatgccgaa
    12181 gatgatgtcg cgcacgccat attggccgcc atatttgatc accgcctcga gcgccggaat
    12241 catcgcttcg cccccgtcga ggccgaacct tttggtgccg acatatttgc gggcgaggaa
    12301 ccgctcccac tgctcgccct cgatcacctt ggtcaggatc gactgcttgc cctcgggggt
    12361 gaagcggatc gccgcctcgc gcccttccat ccggtcctgc aggaagcggc gctcgtcgag
    12421 gtcgttgatg tgcatatatt cgaggccgac atggccgcaa taattggcgc gcaggatgtc
    12481 gacgacctcg cgcaccgtcc cctgctcgag ccccagcgcg ccgccgagat agatggtgcg
    12541 gtcgagcgcc gcgcccgaaa agccgtgata ttcgggggtg agttcggtcg gcatctcgcg
    12601 cttgacgagg cccaatgggt cgaggtcggc ggcgagatgc ccgcgcacgc ggtaggtgcg
    12661 gatcagcatc atcgcgcaga tcgaatcgtt cgccgcctgc ttgatctcgg cgtcggtctt
    12721 gccggccgcg gtcgcgacct ccttcgcctt ggcggccacc gcctcgatcg acatcgacag
    12781 gggatcgagg ccggcattga cctcgtccag ctcgctcacc ggccagttgt cgcgcgccca
    12841 gctgggccct tgttcgtgtt cgccgaacct atccacaatg cttcgccttc tcgtcgtgcg
    12901 cggtcatgaa cgcgccgagc ggttcgccta tccctcagcc cttcaagagc tcgctgaggg
    12961 tcgtgccaag ctcagatggg ctcggcgaca cgcgaattcc agccgcctcc atcgcggcga
    13021 tcttgtcgtc ggcgccgccc ttgccgccgg cgacgatcgc gccggcatgg cccatgcgcc
    13081 gtccgggagg cgcggtccgc ccggcgatga agccgaccat cggcttggcg cggccgcgcc
    13141 tggcttcgtc ggcgacgaac tgggccgcat cctcctcggc cgatccgccg atctcgccga
    13201 tcatgatgat cgactgggtc tcgtcgtcgg cgaggaacat ctcgagcacg tcgatgaagt
    13261 tggtgccgtt gacgggatcg ccgccgatgc cgaccgcggt ggtctggccg aggccggcat
    13321 tggaggtctg gaacaccgct tcataggtga gcgtgcccga acggctgacc acgccgaccg
    13381 agcccttgcg gaagatgttg cccggcatga tgccgatctt gcactcgccc ggcgtcagca
    13441 cgccggggca gttcggcccg atcagccgcg atttggagcc ggtcagcgcc cgcttcacct
    13501 tgaccatgtc gagcaccgga atgccctcgg tgatcgcgac gatcagcggc acttcggcgt
    13561 cgatcgcctc aaggatcgaa tcggccgcga aggccggcgg cacgtagatg acggaggcgt
    13621 cggcccccgt cgcctcgacc gcttcatgga cggtgtcgaa gaccggcagc ccgatatggg
    13681 tctggccgcc tttgccgggc gtgacgccgc cgaccatctt cgtgccgtag gcgatcgcct
    13741 gctcggaatg gaaggtgccc tggctgccgg tgaagccctg ggtgatcacc ttggtggtcg
    13801 cgttgacgag aatggacatt caaaagctcc aagtggcgcc cgccggtgga cgggtgatcg
    13861 ttccgttata ttctagcgat gtgagttgcc gcttattgat gtcgccggtg caggacaacc
    13921 gctgtgcgac ctctgccggc cttgccccaa gctccatcta catcacgaac tgcccggtat
    13981 cgacgatccg gaagctgtgg cgctgcccct ccgacgacga tccgctatcc gccgcctcac
    14041 tccgaggccc ctcccggagc tgcgggaaaa cgtcggaagc cttgcgatgc acgcaagtcc
    14101 cgcgtccagt cgcgcctctt ccgccggcgt catctcgccg catgcgctga ccaacaacag
    14161 gggcaggagc gtcaagcgcg ccgcggattt ccaatccaaa gctgcgtccg cgaccttggc
    14221 agctccagac ccgaccggct cgcccgacgt gggtgcgcgg cgccaaggcg acggctcgag
    14281 agaatggctt gcggccgtca ttcagcccgc tccctgccgg gccttcgctt cgagcgcttg
    14341 cggtcccaat atttacgctg cgcctccgcc cgtctcgcgg cttccgcctc atcgaggacg
    14401 gtcgtgctca tgacggtata ggggtgggtt cgccagaccc gcatgacata gcggtgctgc
    14461 ggggcgtcca tttgccatgc cacggttttg ctggaagcgg ggtttcttat tcggcctcca
    14521 ctcagcaggc cggtaaggcg cccgatggcc cgcgaggggg cgacaaatct cgatgcgaga
    14581 atgcattgat cctcgatccc gtggcgttgc ggatcttgat atttcagcgt cacgaggaag
    14641 gccttggcgg gcgaatcttt aaggattcat cggatcgtgg atcgtccact ccagagtctc
    14701 cttatttttt ctttttattt tctatcagct tatccacgcg ccgtctccgg atgagggaag
    14761 attccctctg ctgttcggtc atccacccga tttgcacgac gctgtagccc cctacgccgg
    14821 aggcatagga cagggtgtag ccgtgctccg ggcgttcgaa catgagttgc gtcgggttgc
    14881 caacgtgttc agccgcaggc ccctcatagc tcacgcggtc gatctcaagg gagatgcgcc
    14941 tccacgcgaa gccgatatcc acgatttgcg aagcaatgcc gcaaacacga gcaaatctcc
    15001 gtggatgcgg agattggtcg aaccagtaca ggaatgacga ggctgacccg gccagcttga
    15061 cgaacgtccc tccggcagga cgatattcgc gcctcagctg cttgggcagg tgctccaacg
    15121 tgaccgagat gctttgttcc ggtttcagct tcgcttctcc gcgcaagcag gctgctatga
    15181 atacgtccac gatgcgggct tgctcgaggg ccagcggagc cccaggcggt ggagcgaggt
    15241 ccatcatcct atgccagggt cgggtcgatg cccttgcagg cgacaagcag cctcgtcccg
    15301 taggcgatcg cctgctcgga atggaaggtg ccctggctgc cggtgaagcc ctgggtgatc
    15361 accttggtgt tcgaattgac gagaatgctc attccgcgcc gctttccttg tattttgccg
    15421 ataagaccaa gccgctaaag tcgactccgc cgaactttgc ggcgccaggg gcgaagaaga
    15481 tgatcgtatc cgatgcgtgg ccagggcgga gcccaggcgc ccaagccatc accgaacggc
    15541 cagaagattt ctcggccatc tcggcgggcc gcccggccca ctcccgagcc tgcgccggat
    15601 cgaccccgct ctgcgcatcg aagtcgtaga caacgcagct cagccggccg gccccgtcgg
    15661 tcgtggagga gtcggtcagc aggagatgaa gagttctgcc tgccacggcc ttctggtacg
    15721 tcttgaacct caagcttgca gactgcgacg tcatggcacc caccagggag tggagtcgac
    15781 cgcccgacac ggatttgtgg acggtccacc ctccagcgac gattgcctcc tcctgccggc
    15841 cgtgggaagc gctcacggca caggctttgt cgaactcggc gagtatcggt gcagcgggat
    15901 agtccgcgcc gaggcccgcc agtcccgcca aggctagaag cagggatgtc aagcgaggct
    15961 ctcgtcgatg cccttgcagg ccaccagcaa ttccttgacc gcgtcgaccg agacgtcgaa
    16021 attctgccgc gccgtctcgt cgagctcgat ctcgacaatg cgctcgactc cgccggcgcc
    16081 gatcaccacc ggcacgccga cataaaggtc gttcacccca tattggccgg tgaggttggc
    16141 cgccgcgggc aatatccgtt tcttgtcctt caaaaagctc tcggccatca taatcgcgct
    16201 ggtcgccggc gcgtagaagg ccgatccggt cttcaggagc ccgacgatct cgccgccgcc
    16261 tccgcgggtg cgctggacga tcgcgtcgat cttttcctgg ctcgaccagc ccatcttgat
    16321 gaggtccggg atcgggatgc cgccgaccgt cgaataagcc ggcaccggca ccatcgtgtc
    16381 gccatggccg ccgagcacga aggcggtgac gtcctcgacc gagacgttga attcctcggc
    16441 caggaaatgg cggaaccgcg ccgaatccag cacgccggcc atgccgacca ccatgtggtg
    16501 cggcagcccg gagaattcgc gcagcgccca caccatcgcg tcgagcgggt tggtgatgca
    16561 gatcacgaac gcggtcgggg cgtgggcgcg gatgccttcg ccgaccgcct tcatcacctt
    16621 caaattgatg ccgagcagat cgtcgcggct catcccgggc ttgcgcggca cgccggcggt
    16681 gacgatgcac agattggcgc cggcaatgtc ggcataatcg ttgctgcccc tgagctcgac
    16741 gtcatagcct tcgacgggcc ccgcctgggc gaggtcgagc gccttgcctt gcgggacgcc
    16801 ctcggcgacg tcgaacagga tgatatcgcc catttcgcgg atcgccgcga ggtgggcgag
    16861 ggtgccgccg atcatgccgg cgccgatgag cgcgattttc ttgcgagcca tgggggctgc
    16921 tcgagccttt ctgtctgggg ccggctgcgg gaacagccgc gggatgccgc gtcgcttacc
    16981 cccggcacgt ccgaggggca accgccctgt tgcttacggc tttcactcct ccccgtgccc
    17041 ccgtgccaga tagtcttccg actgcatctc gatcagacgc gaagcggtgc gctcgaactc
    17101 gaacgcaccg tcgccggccg tatagaggtc cgccggcgtc gcatcggcgg cggcgagcag
    17161 cttcacctta tgctcgtaga gcgcgtcgat tagcgtcacg aaccgcgccg cctcgttgcg
    17221 gttttccggc cccagcttcg gaatgccgac caggatcact gtatgatagc gccgcgcgat
    17281 ggcgagatag tcgggcgccc cgcgcgcctc tccgcacagc cgcctgaacg agaagaccac
    17341 gacccctttg agcgatttcg gcacgtgcag cgtgcgtccg ccctgcacgg gaatatcctc
    17401 cgacggcacc ttgtcgcgat tctcgactgc ataatcggtg aggcggaaga aggcctcgct
    17461 cagcgcccgc gtcgcctccg gcccgttcgg cacatgccag gtcggcagcc cgccgagccg
    17521 ctcgagccga tagtcggtcg gtccattgag cggcaccacc tcgagctcgc gctcgaccaa
    17581 gtcgatgaac ggcaggaaca gctcgcggtt gagcccattc ttgtagaggt cgcccggcgg
    17641 ccggttggag gtggtgatca cggtcacgcc ctcctccagc agccgcccga acagccgcga
    17701 caggatcatc gcgtcggcgc tgttgttgat caccatctca tcgaaggcga gcaaacgcgc
    17761 ttcggccgcg atagcctccg ccaccggcgg gatcgggtcg ccttcctcct tggcgcgctc
    17821 ggcccgcagc cgctcgtgca cttcgagcat gaattcgtga aaatgcaccc gccgcttggg
    17881 cgcgaaggcg atcgcctcgt aagcgaggtc catcagcatc gatttgccgc ggccgacccc
    17941 accccacaaa tagagcccgc gcaccacctt gggcgccttg ccggcgagct tcgccagcag
    18001 cccgcgtttg ggtttggcct caagctcggc agcgagccgg tcaagatgct cgaccgcgcg
    18061 gcgctggtcc tcgtccggcc gcagctcgcc cgcggtgagg agtcgctcgt agcatccgcc
    18121 gacagggccc aagacgtatc tcattcggct cgggcagtca gaccatctct catgcgtttg
    18181 cgtccacaac gggagttggg ttttgagccc tcccgagtga acgcagcaaa tagctgaggc
    18241 tggcgtccct catgctccac atgaaaagtc tcgcgatgtc tcgtttcgac aggtgtatca
    18301 tgaacatgta ttcgccgttt agagggcgag atattcccac tcgaaaatag agcttggcaa
    18361 aacctgcatg ctgccgtgac ggtggtcggt cagatgtttc gacaacgatc ccctcgtggg
    18421 gaactcgatc cgtggcgcta ccaagagcgg cgtctgtgag tgagccgacg aagatatccc
    18481 tccatccgtg gtttcgatgc caacctctgc aagacagccg catgcgtccc tccttcaatc
    18541 aacagggact catatcagct ctagcacact cagggaagcc ttaaataata caactggaac
    18601 ttgagctaga tagcccgctc gagcagcagc ttcttggtct cggcgatcgc cttggccggg
    18661 ttgaggccct tggggcagac gttggcgcag ttcatgatgg tgtggcagcg gtagaggcgg
    18721 aacgggtcct cgagctcgtc gagccgctcg ccggtgcgct cgtcgcggct gtcggccagc
    18781 cagcgatagg cctggagcag gatcgccggg ccgaggaagc ggtcggaatt ccaccaatag
    18841 ctcgggcagg aggaggagca gcaggcgcac catatgcact cgtacagccc gtccagcttg
    18901 gcgcggtcat cgggcgactg cagccgctcc ttgtcggacg gggtcggcgt caccgtcttc
    18961 agccacggct cgatcgaggc atattgggcg aagaagtggg tgaagtccgg caccagatcc
    19021 ttgatcacgt ccatatgcgg cagcggcgtg atccgcactt cgcccttcac gtcctcgatc
    19081 gcggtcgtgc aggcgaggcc gttgcggcca tcgatgttca tcgagcagga cccgcaaatg
    19141 ccctcgcggc aggagcggcg gaaggtgagg gtggggtcga tctcgttctt gatcttgatg
    19201 agcgcgtcca gaaccatcgg cccggtctcg tccaagttga tctcgaacgt gtcgtagcgc
    19261 ggattctcgc cgctctccgg gtcgtagcga tagactctga cccgcttgta gcggccgggg
    19321 ccgcccgcgg ccttgtggac gcggcccttg cctttgatct tgctgttctt gggaagggcg
    19381 aattcggcca tgtcgatctc ctgatgcggc gcatctagcc gctggcgcgg gcgcgctcaa
    19441 tgccggacgg gtggcaatcc tggacggccg gctcaggcga cgcgcgcgta acgctccggc
    19501 cgcgccgccc gcttgcccag caggtcgttg cgcctgaggc tttcgcacag gatcgtctcg
    19561 atgagctcgg catggctcca tccggccagc cgcgccgcct tgctgatcgt cttttccgac
    19621 cacagattgc aattgaggtt cacttccagg aactgaacct ggccgctctt ataatccatg
    19681 cggaattcga agcggccata atcgaacggc aggaattcgc gcatgatgtt gcgggcatgc
    19741 tcctcgatca ccggcagcca gcgcgcgtcc cggaacggca atatctcgta gcgctggccg
    19801 ccggcgaggt tgcgcttctc attataggtc cgcagcaatt ccgggtcgct ctgctcgacg
    19861 atcatggtcg gcaggatcag cggctcgcca tcgaccgtga tcaccgaaac ttcgatgtcg
    19921 gagccggtga tgaacggctc gacaatcgcg tcatggccct gctcgtgaag ctcggcgacc
    19981 gcgcggcgga cgccgtcgag gtcgtgcgcg tcgctgacgc cccagcttgc cgacgacgcg
    20041 ttcggcttca ccacataacg ctgcgcccgc gggcaatgcg ccgtgtcgat cgcggtgccg
    20101 cggcggtaga tcgcccaagg ggcggtggga atgccgcggg cggcggcggc gagcttggtg
    20161 aggtgcttgt cgtcggagac gccgcgcagg atcgggctag cgccgaggaa cgggatgccg
    20221 agccggttgc acagcaaggg cagcagcatt tccgaattga ggaagccgcc ccggttgagc
    20281 agcggaaaca cgaagtcggc gccgggatgc tcgaacaatg agcgatagct gtcggcgagg
    20341 atcaggttga ggcccagctc ctccaatatc cggcgtatct cctggtggta gatggcatgg
    20401 ctgccatcct ccggatgaag gccgccgcgc caattggcgt gcttggcgat gaacatgatc
    20461 cgcagcgatt cccgcgcgga ccggggaatg gcgagcggcc gagccgatcc gttcacgcaa
    20521 caataccttc ttgatttata tcaattattg ggagattcga gcggtgcttc gcggcgcccc
    20581 atagccgctt ccgccggaag cgccaagagg tgcggcccgt acatttggcg agcggcagct
    20641 ttactgcaga tgaagcgccg gcgcaggcgg ggcagggggg ccgcgcatgc gcatcaccac
    20701 cggcatcgat gcgaagaccg aaggctgcag gcgccgcagg tggaaggtca tgaggtcgcc
    20761 gagccggtgc ccggctatgc cggtgccgct gaccagttga agcagcggcg agcccgggtt
    20821 ccaattgccc tcgatcagca ttgcgccgtc ctccgacagg ccgatgtccc agccgatcag
    20881 taccctgcgc ccgccgaaca ggcgccggtg ggcctccgcc gcgagcgcca ccgccgcctg
    20941 gaagagcggc acctcccgcc cctcgatcgc cgcgtccgtg accgggtgcc ggctgatccg
    21001 gtcgacgcgg ccgccgcggc cgtgcctgat gcgggcttcg gcgaggatgc cggtgccgag
    21061 attggcggcg gccgagatgc cgccggcgct gaaattgtcg agcgccgacc cgatcggccc
    21121 cagccggagg aaggcgtggg tcggctccgg ctcgccgcgc tcgtcgaggc agacgatcac
    21181 ccgcagcgtc ggcagcgccg tcggcgacag gtcggcgagc gccgcatggg tccgcagccg
    21241 ccgctggacg tttccggccg ggtcgagatt cgcccgcagc cacccgatga gatcaacgac
    21301 cggacccccg tccgcccgcc attccgcccc ctcccgcgcc agcgccgcga tgcccttgcc
    21361 ctgcaggctg tagccgcgct tgaggatcag gccggagccg ctctcgatcc aggccgggag
    21421 cggtgcatcc agtgcgcccc gcccgatcgt ctcgggcagc ggcagcccgg cggcgcgggc
    21481 gaggcggtcg aaatgacatt tgtcggacag cgcgcggtcg tcgcccgcgc cttcaggatc
    21541 gagcagggcg cagatcgccc gcttcgcccg cggcgagacc cagctctgct ccgcgcccgg
    21601 cccgatattc tcccgcgcct cgtcgaggtc gcgcggcacc agcccctcgc gcaggcacag
    21661 ccgaacgtcg ctccaccgcc gcggccccca gcgcctcagc gccagccaaa ccactcaagt
    21721 cgctccaccc cagcaccagc gcattcagga tcgccccgcc ccagccccgc gcctccgcca
    21781 gccgccaggc cagcacgtcc gccgggtgca tcgaaccacg ccgcccgcgc gccgcgcgcg
    21841 ccggatacgc cgcgaacgtt atccagccca cgggctggct cggataagtg cgaagaaagg
    21901 cacggtgcgc gcggttaggc cgccggccgg ctcgccacaa gcgttcagcc gtcggctccg
    21961 ccggggcagt agcggacccg ctcccccggc ttcccccgca atcgcgtttt cagataatat
    22021 ccaagcatgg cgtcggcctg cctgccctcg tccggcgcaa gcaaggccgc ggcgtcggac
    22081 tgccatcccg caatgcaggc gccgtcgccc cggctcgatc cggcgcagtc gtggagccgc
    22141 tcgaacgcgc gcttccagac catgaattcc ggccgtccca tcaccgcgcc atgtccggga
    22201 acgaggcgag tgaacggcgt ccccgctatc tccgtcagcg cgcggcgcca cccctccggg
    22261 caggcggtgt cgaagaacgg caccggcaag gtgactagat cgccgaccgc cacgattccg
    22321 ctggcggcat cgtagaccca gacgtcgccg gcggtggcgg cgtgctgtgc cagccgcagc
    22381 gacagcggac gccctgcgat cctccgttcc cccggcccgt ccacaggggc ggtggggagc
    22441 agcgagcggc gctcgtccac ggccgcgata tcgccctcga tttcggccgc cgtcgcggcg
    22501 tccgccttgc cggacgccag gaaagcgacc gcttccgcct tgctcctggc taggaagccg
    22561 gtcagcgctc cttccaccgc acggctggcg tggatcgaga gctcaccccc ggccgcctcc
    22621 cgcagctcgg cattgccgcc gctatggtcg aggtgccagt gggtgtttac cagcgccgcg
    22681 atcggcatgc gccgcgccgc cgcgagcgcg aggatcttgc gctggtgctc ggcatggcgg
    22741 ccgctgtcga ccacgatcag cccgtccggc gcctcgaaga cgacgctgtt cccgtccggc
    22801 tgccgccccg ccccgaagcc accttgaatg aagtggactc cgtcgccgat cggcaccgcc
    22861 gccggggccg gcgccagcgc ggtcagatag gcaatggcca gtcgaacgat catgagcggc
    22921 ggaagctagg ctttcccggc agcgtcggga ggtccgcttc tgccggcggc cgcccctttt
    22981 cgacgaaccg ccgccttcag cagtcgacgt ggcgccgctc ccaggtccgc ttcggatcgt
    23041 gggcgcgaag gtcgatgccg aaatccaccg ccgccttgaa cgccagcagc acgctcaccc
    23101 agccggcata attttcgaga tgctcctccg ccggcacgcc ctcttcgcgc agataaaggt
    23161 cgcagccgcc gcggccgtcg ggctcgaagg cgagggtgac ggtcgatccg ccgaaatagc
    23221 ggaagaccag ccgcgtcgcc ggcatcgcct ccaccacctc cacggccaag gcctggccgt
    23281 tgacgaagcg cagctcgaac ccgcccgggc cgctccgcga ttcctcagcc cagaacctgc
    23341 tccgcccggc atctttggtc catgcttcgt aagccgcctc ggggctgctg cgaacatgga
    23401 cccgccactc gatcggcggc gcgccgtcca tcaataaacc cgcgccttgg gcttgatata
    23461 ctggatctcg tcggtgagcg tgtaatcgtg caccgggcga taatcgatcg tcaccgcgct
    23521 gccctggccg aaccaggcga tggtgtgctt catccaattg gcgtcgtcgc ggttcggata
    23581 atcctcgtgc atgtgggcgc cgcggctttc ctttcggttg gccgccgagt gcatcgtcac
    23641 caccgcctgc ggcagcatgt tgtcgagctc gagcgtctcg atgaggtcgg tgttccacac
    23701 gaggctgcgg tcggtgacgc ggatgtcgcc catgcgccgc tgcacctcgt cgatcttggc
    23761 cttgccctcg gcgagcgtcg cttcggttcg gaacacggcg cagtcggcct gcatcacctt
    23821 ctgcatggcg gcgcggatct ccgccgtcgg ggtcggaccc ttggcgtggc ggaagccgtc
    23881 gagccgggac agcggcattt cctcggagcc gcgcgggagc ggccgctgcg cggcgcccgg
    23941 cttcaacagc tcgccgagcc ggtggccggt cgcgcggccg aacacgacca gatcaatgag
    24001 gctgttggag cccaatcgat tggcgccgtg gaccgacacg caggccgcct cgccgaccgc
    24061 atagagaccc ggcacggtgg cgtcgggatt gccgtccttg agcgtgacga cctcgcccca
    24121 ataattggtc gggattcc
//
LOCUS       DASVRK010000291         8103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_494326, whole genome shotgun sequence.
ACCESSION   DASVRK010000291 DASVRK010000000
VERSION     DASVRK010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494326"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VF589_12145"
     CDS             <1..1357
                     /locus_tag="VF589_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX8258373.1"
                     /translation="YSIGLASYLAVLEALWLRTGKPVYLALFRYWLKIFAIAFGMGVV
                     SGIVMSYQFGTNWSVFSDRAGPVIGPLMAYEVLTAFFLEAGFLGVMLFGLERVGKKLH
                     FVATLMVATGTAISAFWILSVNSWMQTPTGWAVNAQGQFVPAGSWLDIIFNPSFPFRL
                     VHTVIAAYLTTALVVGGVGAWHLLRGHGTAEVRKMFSMAMWMAALVAPVQIVVGDFHG
                     LNTLEHQPAKVMAMEGHFDSYPNGAPLILIGMPDSEAEKTRVLLEIPKLSSLILKHDA
                     NAPLEGLKSFPKDQRPPAEIVFWSFRVMVGLGMLMLALGLFSLFARLRGKLHDWRWLH
                     RFALVMGPSGFVAVIAGWITTEVGRQPWVVYGLLRTADARSPIAAPAVATSLLAFVIV
                     YFIVFGIGTWYLLKLMHHPPHAHETGEAHAPIRTAGITPAGAVHRPGADPRPDATEEA
                     Q"
     gene            1489..2490
                     /gene="cydB"
                     /locus_tag="VF589_12150"
     CDS             1489..2490
                     /gene="cydB"
                     /locus_tag="VF589_12150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX8258374.1"
                     /translation="MDLTVIWAFLIAFAVAAYVVMDGFDLGIGILFPGFTVGKERDQA
                     MNAIAPVWDGNETWLVLGGGGLMAAFPLAYAIILPALYAPLIAMLLGLVFRGVAFEFR
                     WRDPGHRKWWDGGFFLGSLVATFAQGVTLGALLQGISVEGRAYAGGWWEWLTPFSLLT
                     GASLVVGYALLGAAWLIWKTEGRIQADARRFAVILMPTLLVAIGIVSLATPFVEPKYL
                     TRWFEWPGLLLSVLMPLLVGGNALALWWSLGWRKDWLPFVLTLGLFLLSMAGLAISIW
                     PDVIPGRVSIWQAAAPYQSQLFMLIGASVLIPLILAYTAWSYWVFRGKVGEEGYHEH"
     gene            2480..2599
                     /locus_tag="VF589_12155"
     CDS             2480..2599
                     /locus_tag="VF589_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 domain-containing protein"
                     /protein_id="HEX8258375.1"
                     /translation="MSIDAPAPLWRRLAWFVGLWVAGVATVGSVAWLIRAWLL"
     gene            2635..2964
                     /locus_tag="VF589_12160"
     CDS             2635..2964
                     /locus_tag="VF589_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX8258376.1"
                     /translation="MAALGVACGAFGAHGLRAVLGAAELGWWQTAVQYQMWHAVALVA
                     LALLPRPRLPALLLAGGILIFSGTLYLMALTGWRWLGAVTPLGGLMLIGGWVLVLWRT
                     ARGGGSV"
     gene            complement(3166..4533)
                     /locus_tag="VF589_12165"
     CDS             complement(3166..4533)
                     /locus_tag="VF589_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8258377.1"
                     /translation="MAWNLILSLTLAAAQPAAAETIVIRGATVIDTVGERDIVNATVV
                     VSNGRIAAVAAGAPVPAGARVIEARGKYLIPGLIDGHVHFFQSGGLYARPDAVDLRSQ
                     VPYAQEVAAVKRRMPETLRRYTRAGITGAADMGGPMWNFELRAAAARQPDAPRVWVAG
                     PLLSPVQPEALVTDDPPILKTNNAAEATAQARTQLARKPDLMKFWYIVGGEGPAPVRA
                     RLAAMVAEARRAGVKAAVHATEQETARAAVAEGADILVHSVDDQEVDAALVEAVRRRG
                     AVYIPTLIVMGRYGDVYGRRHPFPAAERAAADPDVSASFDALAKAELPDWAPKASEHM
                     PTLLPRMQRNLMRMYRAGVPIVMGTDAGNIGTPHAVSVPAEMAAMAEAGMPARAVLAS
                     ATIAPARMLGAERELGSIEAGKVADLVLLTEDPRRDVRAVAAVEMVVRGGRVIGESGS
                     EGVQQ"
     gene            complement(4627..4995)
                     /locus_tag="VF589_12170"
     CDS             complement(4627..4995)
                     /locus_tag="VF589_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8258378.1"
                     /translation="MADVKPTHLAQVAVTIRDLERAKAFYRDALELKHLFDAPPGLAF
                     FQCGQTRLMLSQPEGPETVGNSILYYGVEDVAAAHRALAGKGVAFEEEPRCIAKIEGR
                     DVWLAICRDSEGNLLGLMSG"
     gene            5128..5496
                     /locus_tag="VF589_12175"
     CDS             5128..5496
                     /locus_tag="VF589_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8258379.1"
                     /translation="MLRLLRREDEGSGITGPRLSALSVIVFAGPVTMAELAAAEQVRA
                     PTMTRIVDALVADGLAERAADPADRRLVRVVATDQGRALLEAGARRRVRALTDRLGKL
                     AESERRALERGVEILERTVR"
     gene            5591..7441
                     /gene="typA"
                     /locus_tag="VF589_12180"
     CDS             5591..7441
                     /gene="typA"
                     /locus_tag="VF589_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX8258380.1"
                     /translation="MSLRNVAIIAHVDHGKTTLVDQLFRQSGTFRDNQRVEERAMDSN
                     DLEKERGITILAKCTSVEWEHDGETTHINIVDTPGHADFGAEVERILSMVDGVILLVD
                     AAEGPMPQTKFVTGKALALGLKPIVVVNKIDRPDARPAEVLDECFELFLSLDANDEQL
                     DFPTLYASGRNGYAGLTDDVRSGDLTPMFEKIVDHVPAPGLDPEGEFAMLTTLLDRDN
                     FLGRILTGRIESGRLKVNMPIKAIDVDGKVVEEGRATKVFAFRGLDRVPVDEAQAGDI
                     VAIAGLTRATVSNTIAEPARAAPIAARPIDPPTLAMSFAVNDSPYAGRDGDKVQSRVI
                     RDRLEREAESNVAIRLTESQERDAFEVAGRGELQLGVLIETMRREGFELSISRPRVLF
                     QDGPPNPDGSRAREEPYETVVIDVDDEHSGTVVEKMAIRKGEMTDMRPSGGGKTRLTF
                     SAPSRGLIGYHGEFLSDTRGTGIMNRLFEKYGPYKGPIQGRQNGVLISMEQGEAVAYA
                     LNMIEERGILFISPGDKLYQGMVIGENAKPQDLEVNPLKSKQLTNFRASGPKDESIRL
                     TPPRRMTLEQAIAYIQDDELVEVTPKVIRIRKRHLDPHERKRASRRSEAA"
     gene            complement(7707..>8103)
                     /locus_tag="VF589_12185"
     CDS             complement(7707..>8103)
                     /locus_tag="VF589_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258381.1"
                     /translation="IPDDFAPDNEELRLLAKQFYTDLRETWERAVEEVVLGGVVERFG
                     SDVKTQSLKRVDIDDADYRVIFFAMKRASERSGHDQATAKQIDPPNKKQMESDLLELR
                     GFIATHRKKAQAADERRKELEQPPKAAVA"
BASE COUNT         1216 a         2932 c         2593 g         1362 t
ORIGIN      
        1 ctattcgatc ggcctggcca gctacctcgc cgtgctcgaa gccctgtggc tgcggaccgg
       61 caagccggtc tacctcgccc tcttccgcta ctggctgaag atcttcgcga tcgccttcgg
      121 catgggcgtc gtctccggca tcgtcatgtc ctaccagttc ggcaccaact ggtcggtctt
      181 ctccgaccgc gccggtccgg tgatcggccc gctcatggcc tatgaggtgc tgaccgcctt
      241 cttcctcgag gccggcttcc tcggcgtgat gctgttcggg ctcgaacggg tcggcaagaa
      301 gctccacttc gtcgccactc tgatggtcgc caccggcacc gccatctccg ccttctggat
      361 cctcagcgtc aacagctgga tgcagacccc gaccggctgg gcggtcaacg cgcaaggcca
      421 gttcgtgccg gccggcagct ggctcgacat catcttcaac ccgagcttcc cgttccgcct
      481 cgtccacacc gtcatcgccg cctatctcac caccgccttg gtggtcggcg gcgtcggcgc
      541 ctggcatttg ctgcgcgggc acggcaccgc cgaggtgcgc aagatgttct cgatggcgat
      601 gtggatggcg gctctggtgg cgccggtgca gatcgtggtc ggcgactttc acggcctcaa
      661 cacattggag caccagcccg ccaaggtgat ggcgatggag gggcatttcg attcctatcc
      721 caacggcgcc cctttgatcc tgatcggcat gcccgacagc gaggcggaga agacccgcgt
      781 cctgctcgaa atccccaagc tctcgtcgct gatcctgaag cacgatgcta acgcgccgct
      841 cgaaggcctg aaatcctttc cgaaggacca gcgcccgccg gccgagatcg tgttctggtc
      901 gttccgggtg atggtcgggc tcggcatgct gatgctggcg ctcggcctgt tcagcctgtt
      961 cgcgcggctg cgggggaagc tccacgactg gcgctggctg caccgtttcg ctttggtgat
     1021 gggcccgtcc ggcttcgtcg ccgtgatcgc cggctggatc accaccgaag tcgggcgcca
     1081 gccctgggtg gtctacggtc tgctccgcac cgccgacgcc cgctccccca tcgccgcgcc
     1141 ggccgtggcg acctccttgc tcgccttcgt gatcgtctac ttcatcgtgt tcggcatcgg
     1201 cacctggtac ctcttgaagc tgatgcacca ccccccgcac gcccacgaaa ccggcgaagc
     1261 ccacgccccc atccgcaccg ccggcatcac ccccgccggc gccgtccacc gccccggcgc
     1321 agacccccgc cccgacgcca cggaggaagc gcaatgatgg ccaccgcaaa tcctccccgg
     1381 aacggggtgg gggaccgcga cgcgaagcgg cgtggtggag ggggccctcc atgcgcgcgg
     1441 cgggtcccgg ggcccccaac accctccccg ttccggggag gagcctaaat ggacctcacc
     1501 gtcatctggg cgttcctgat cgccttcgcg gtcgcggctt atgtcgtgat ggacgggttc
     1561 gatctcggca tcggcatcct cttccccggc ttcaccgtcg gcaaggagcg cgaccaggcg
     1621 atgaacgcca tcgcgcccgt ttgggacggc aacgagacct ggctggtgct cggcggcggc
     1681 gggctgatgg ccgcctttcc gctcgcttac gcgatcatcc ttcccgcgct ttacgcgccg
     1741 ctgatcgcga tgctgctcgg cctcgtcttc cgcggcgtcg ccttcgagtt ccgctggcgc
     1801 gatcccggcc accgcaaatg gtgggacggc ggcttcttcc tcggctcgct ggtcgcgacc
     1861 ttcgcgcaag gggtgacgct cggcgcgctg ctccagggca tttcggtcga gggccgcgct
     1921 tatgccggcg gctggtggga atggctgacc ccgttcagcc tgctgaccgg cgccagcctg
     1981 gtggtcggct acgctttgct cggcgccgcc tggctgatct ggaagaccga aggccgcatc
     2041 caggccgacg cccgccgctt cgccgtcatc ctgatgccga cgctgctggt cgcgatcggc
     2101 atcgtcagcc tcgccacgcc cttcgtcgag cccaaatatc tcacccgctg gttcgaatgg
     2161 ccgggcctgt tgctgtcggt gctgatgccg ctgctggtcg gcggtaacgc gctcgctctg
     2221 tggtggagcc tcggctggcg caaggactgg ctgcccttcg tcctcaccct cggcctgttc
     2281 ctgctgtcga tggccggcct cgccatctcg atctggcccg acgtcattcc cggccgcgtc
     2341 tcgatctggc aggcggccgc gccctatcag agccagttgt tcatgctgat cggcgcctcg
     2401 gtgctgatcc cgctgatcct cgcttacacc gcctggtcct attgggtgtt ccgcggcaag
     2461 gtcggcgagg aggggtatca tgagcattga tgcgcccgcc ccgctgtggc ggcggctggc
     2521 ctggttcgtc ggcctctggg tggcgggcgt ggccacggtc ggctcggtcg cctggctgat
     2581 ccgcgcgtgg ctcctgtgac cttcgaccgg ctcgtcctcg gcgccggcgc cctcatggcc
     2641 gcgctcggcg tcgcctgcgg agccttcggc gcccacggcc tgcgggcggt gctgggcgcc
     2701 gccgagctcg gctggtggca gacggcggtc caatatcaga tgtggcatgc agtcgctctg
     2761 gtcgcgctcg ccctgctgcc gcgcccccgc ttgccggcct tgctgctcgc cggcgggatc
     2821 ctgatcttct cgggcacgct ctacctgatg gcgctcaccg gctggcgctg gctcggcgcg
     2881 gtcactccgc ttggcgggct gatgctgatc ggtggctggg tgttggtgct gtggcggaca
     2941 gcgcgaggtg ggggcagcgt gtgatcgtac aacctaaccg ctcatcctga ggagccggtg
     3001 agcttgtcga accatgaaca ggtcggattg cccccggcaa tcctgaaaca tgccgggggc
     3061 atgtttcacc tgttcattct cgaaggacgc accatgatcc tttgagacgg gccttcgccg
     3121 gggctcagtc cctcctcagg atgagcggag gttgtacgcc tcaagctatt gctgcacgcc
     3181 ttccgacccg ctctccccaa tcacccttcc accgcgaacc accatctcca ccgccgccac
     3241 cgcgcggaca tcgcggcgcg ggtcctccgt cagcagcacg aggtcggcaa ccttccccgc
     3301 ctcgatgctg cccagctccc gctccgcgcc gagcatccgc gccggggcga tcgtcgcgct
     3361 ggcgagcacc gcccgcgccg gcatccccgc ctccgccatc gccgccatct cggccggcac
     3421 cgacacggcg tgcggcgtgc cgatattgcc ggcatcggtg cccatcacga tcggcacgcc
     3481 ggcgcggtac atgcgcatca aattgcgctg catgcgcggc agcagcgtcg gcatatgctc
     3541 gctcgccttg ggcgcccagt cgggcagctc ggccttggcc agcgcgtcga agctggcgga
     3601 gacgtccgga tcggcggcgg cgcgctcggc ggcggggaac ggatggcggc ggccatagac
     3661 gtcgccatag cggcccatca cgatcagcgt cgggatgtag accgcgccgc gccggcgcac
     3721 cgcctcgacc agagccgcgt ccacttcctg gtcgtcgacg ctgtggacca atatgtccgc
     3781 gccttcggcc accgccgcgc gcgcggtctc ctgctcggtg gcgtggaccg cggccttgac
     3841 gccggcgcgg cgcgcttcgg ccaccatcgc cgccagccgc gcccgcaccg gcgccggtcc
     3901 ctcgccgccg acgatgtacc agaatttcat gaggtcgggc ttgcgggcga gctgcgtccg
     3961 cgcctgcgcg gtcgcttcgg cggcattgtt ggtcttgagg atcggcgggt cgtccgtgac
     4021 caaagcttcc ggctggaccg gcgacagcag cggccccgcc acccagaccc gcggcgcgtc
     4081 gggctggcgc gcggcggcgg cgcgaagctc gaaattccac atcgggccgc ccatgtcggc
     4141 cgcgccggtg attccggccc gcgtgtagcg gcgcagcgtc tccggcatcc gccgcttcac
     4201 cgccgccacc tcctgcgcgt acggcacctg gctcctgaga tcgaccgcgt cgggccgagc
     4261 gtaaagcccg cccgactgga agaaatgaac gtggccgtcg atcaggcccg ggatcagata
     4321 tttgccccgc gcctcgatca cgcgcgcgcc cgccggcacc ggcgcgccgg ccgcgaccgc
     4381 ggcgatgcgc ccgttgctga ccaccaccgt ggcgttgacg atgtcgcgct cgccgaccgt
     4441 gtcgatcacg gtcgcgccgc ggatgacgat cgtctccgcc gccgccggtt gcgcggccgc
     4501 cagcgtcaag gacaggataa ggttccaggc catcgctcgc tccccttagc cgatcctacc
     4561 attccggcgg ccggatcgcc agcggggagg gggcaagcgg gggaggggcc agcggggagg
     4621 gggcggtcag ccgctcatca ggcccagcag attgccttcg ctgtcgcggc agatggccag
     4681 ccacacatcg cggccctcga tcttggcgat gcagcgcggc tcctcctcga acgcgacgcc
     4741 cttgccggcg agcgcgcgat gcgccgccgc gacatcctcg acgccataat aaaggatcga
     4801 attgccgacc gtctcgggcc cctccggctg gctgagcatc agccgcgtct ggccgcattg
     4861 aaagaaagcg aggcccggcg gcgcgtcgaa caaatgcttc agttccagcg cgtcccggta
     4921 gaaagctttg gcccgttcga ggtcgcggat ggtgactgcg acttgggcga gatgggtggg
     4981 cttcacgtcc gccatggcct tgcctccgcg tctgtgctaa atgttcctct ggaacaatca
     5041 ttagtatagc taattaatgg agtcaagccg ctgagcgaga agcgcaccga ggagctggcc
     5101 gggcggctcc attccgcctc gatccgcatg ctgcggctgc tgcgccgcga ggatgagggc
     5161 agcggcatca ccggccccag gctgtcggcc ttgtcggtga tcgtgttcgc cgggccggtc
     5221 accatggccg agctcgccgc cgccgagcag gtccgcgcgc cgaccatgac ccggatcgtc
     5281 gatgcgctcg tcgccgacgg ccttgccgag cgcgccgccg accccgccga ccgccgcctg
     5341 gtccgcgtcg tcgccaccga ccagggccgc gccttgctcg aggccggcgc ccggcgccgc
     5401 gtccgcgcgc tcaccgaccg gctcggcaag ctcgccgaaa gcgaacgccg cgcgctcgaa
     5461 cgcggcgtcg agatattgga acggacggtc cgctgacccg cgcgcgcggc aggcggcgcc
     5521 gccggaccct tgttgcgccg cagcattgcg gctataggcg cgcctcactc gatccatcca
     5581 ggaaattgaa atgagcctcc gtaacgtggc gatcatcgcc cacgtcgatc atggcaagac
     5641 gacgctcgtc gaccagctgt tccgccagtc cggcaccttc cgcgacaacc agcgcgtcga
     5701 agagcgggcg atggattcga acgacctcga aaaagagcgc ggcatcacca tcctcgccaa
     5761 atgcaccagc gtcgaatggg agcatgacgg cgagaccacc catatcaaca tcgtcgacac
     5821 gcccggccac gccgatttcg gcgccgaggt cgagcgcatc ctgtcgatgg tcgacggcgt
     5881 catcctgctg gtcgatgcgg ccgagggtcc gatgccgcag accaagttcg tcaccggcaa
     5941 ggcgctcgcg ctcggcctga agcccatcgt cgtcgtcaac aagatcgacc gccccgacgc
     6001 ccgccccgcc gaagtgctcg acgaatgttt cgagctgttt ctgagcctcg acgccaatga
     6061 cgagcagctc gatttcccga ccctctacgc gtcgggccgc aacggctatg ccggcctcac
     6121 cgacgacgtc cgctccggcg acctgacgcc gatgttcgaa aagatcgtcg accacgtccc
     6181 cgccccaggc ctcgacccgg agggcgagtt cgcgatgctg accaccctgc ttgaccgcga
     6241 caatttcctc ggccgcatcc tcaccggccg gatcgagagc ggccgcctca aggttaacat
     6301 gccgatcaag gcgatcgacg tcgacggcaa ggtcgtcgag gaaggccgcg ccaccaaggt
     6361 gttcgccttc cgcggcctcg accgcgtccc ggtcgacgag gcgcaggcgg gcgacatcgt
     6421 cgccatcgcc ggcctcaccc gtgccaccgt ctccaacacc atcgccgagc ccgcccgcgc
     6481 cgcgccgatc gccgcccgcc cgatcgatcc gccgacgctg gcgatgagct tcgccgtcaa
     6541 cgacagcccc tatgccggcc gcgacggcga caaggtgcag agccgcgtca tccgcgaccg
     6601 cctcgagcgc gaggccgaga gcaacgtcgc catccgcctc accgaaagcc aggagcgcga
     6661 cgccttcgaa gtcgccggcc gcggcgagct ccagctcggc gtcctcatcg agaccatgcg
     6721 ccgcgaaggc ttcgagctct ccatctcccg cccgcgcgtc ctgttccagg acggcccccc
     6781 aaatcctgac ggatcacgcg cccgcgagga gccctatgag accgtcgtta tcgatgtcga
     6841 cgacgagcat tcgggcacgg tcgtcgagaa gatggccatc cgcaagggcg agatgaccga
     6901 catgcgcccg tccggcggcg gcaagacgcg cctgaccttc tccgccccca gccgcggcct
     6961 gatcggctat catggcgagt tcctctcgga cacgcgcggc accggcatca tgaaccggct
     7021 gttcgagaaa tacggcccgt acaagggccc gatccagggc cgccagaacg gcgtcctcat
     7081 ttcgatggag cagggcgagg cggtcgccta tgcgctgaac atgatcgagg agcgcggcat
     7141 cctgttcatc tcgcccggcg acaagctcta ccagggcatg gtgatcggcg agaatgccaa
     7201 gccccaggac ctcgaggtca accccctcaa atccaaacag ctcaccaact tccgcgccag
     7261 cggccccaag gacgaaagca tccgcctgac cccgccgcgg cgcatgacgc tggaacaggc
     7321 gatcgcctac atccaggacg acgagctggt cgaggtgacc ccgaaggtga tccggatccg
     7381 gaagcggcat ctcgacccgc acgagcgcaa gcgggcgagc cggagaagcg aagccgcgta
     7441 gccgccgagc gcagcgccgc cggcgtgaaa cgccgccgaa gcagcgcaaa gagccggctt
     7501 gcgccggccg gcgggtcgct tcagcgaacc cgaccgacgt cacgggattt actcccgtga
     7561 cggcccgccg agatttggcc ccggagaagc gcgtagcaat tcctcccctg catttgcagg
     7621 ggaggggtcc gctcgccgca ggcgagtggt ggaggggccg gcgcgggacg cgccgaagca
     7681 gcgcgaagag acggtaacgc aaacccctaa gcgacagctg ctttgggggg ctgctccagt
     7741 tccttgcgtc gctcgtcagc cgcctgggcc ttctttctat gggtggcgat aaagccccta
     7801 agctccaaaa ggtccgattc catttgtttc ttattaggcg ggtcgatctg cttggcggtg
     7861 gcttgatcgt gccccgagcg ctccgaagcg cgcttcatgg caaaaaagat cacccggtaa
     7921 tccgcatcgt ctatgtcaac tcgcttgaga ctctgagttt tcacgtcgga accaaaccgt
     7981 tcgactacgc caccaagcac cacttcttcc actgctcgtt cccacgtctc gcggagatcc
     8041 gtatagaatt gcttggcaag caaccgcagt tcctcattgt ccggggcgaa atcatcaggg
     8101 atg
//
LOCUS       DASVRK010000292        13349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_121663, whole genome shotgun sequence.
ACCESSION   DASVRK010000292 DASVRK010000000
VERSION     DASVRK010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121663"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..534
                     /gene="paaI"
                     /locus_tag="VF589_12190"
     CDS             103..534
                     /gene="paaI"
                     /locus_tag="VF589_12190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX8258382.1"
                     /translation="MNADEIADKVARAMLSREGTGPAWGIAIEEAREGYARVGMRLRA
                     DMLNGHGIGHGGMVFALADTAFAYACNSRDVRTVAAQASIVFLDAARQDERLTAEAEE
                     QALVGRSGVYHVRVSGEDGRAIAEFQGYARSLGGPVLNSGD"
     gene            546..1748
                     /gene="pcaF"
                     /locus_tag="VF589_12195"
     CDS             546..1748
                     /gene="pcaF"
                     /locus_tag="VF589_12195"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX8258383.1"
                     /translation="MTQAFICDAVRTPIGRYGGALARVRADDLAAMPIKALIDRNGGV
                     DWEEIDDVILGCANQAGEDNRNLARMAGLLAGLPHKSGGVTLNRLCGSGMDAVTMAAR
                     IIRGSEADLVIAGGSESMSRAPFVMAKATSAFDRNAEMYDTTIGWRFVNPKMKGAYGD
                     DTMPSTAENVAQEWQVSREDQDAFAVRSQEKASAAQDNGRFAAEIVAVTIPQRKGDPL
                     VVDRDEHPRKTSVEALAKLRPIVRPDGTVTAGNASGVNDGAAAMIVASEEAARRNGLT
                     PRARIVGGAVAGVEPRIMGIGPAPASQKLLKRLGLSIADMDIIEINEAFAAQALPVLR
                     ELGLPDDAAHVNPNGGAIALGHPLGMSGARLALTATEELHRTGGGYALCAMCIGVGQG
                     IAMVIERV"
     gene            complement(1745..2245)
                     /locus_tag="VF589_12200"
     CDS             complement(1745..2245)
                     /locus_tag="VF589_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vgr related protein"
                     /protein_id="HEX8258384.1"
                     /translation="MVEQVAPGPEDRPLTQAEVELAASVFGDAIDYSPVRIHRRKWFL
                     FQPRNVAMAPCGHIHFHPKGSQHSGDFAADLPWRQALFIHEMTHVWQAQTRGRYYLPL
                     MRHPFCRYDYALKPGQKFELYGLEQQAEIVKHAFMLREGHAVSAAPPLEQYRTLVPFG
                     PWRRRG"
     gene            complement(2239..2715)
                     /locus_tag="VF589_12205"
     CDS             complement(2239..2715)
                     /locus_tag="VF589_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX8258385.1"
                     /translation="MKSLWRLALLFALAPALQGCSKGTAIKNAWIRLPAVAGRPAAAY
                     FDIEVGTGDLRLVAVKGPFERAELHETMEAHGVAAMRPMPYLELHSQSEVSFEPGGKH
                     AMLFGLEPRLRPGGRAELRFAEGNSEGRAIGEIAVEARVVGPADPTPYPKGLFRLW"
     gene            2855..4768
                     /gene="dnaK"
                     /locus_tag="VF589_12210"
     CDS             2855..4768
                     /gene="dnaK"
                     /locus_tag="VF589_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8258386.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGSKPKVVENAEGARTTPSIVAFAK
                     DGERLIGQPAKRQAVTNGDNTIFAVKRLIGRRFDDPITKKDTELVPYTITKGPNGDAW
                     VKAGGQDYSPSQISAFILQKMKETAEAYLGETVTQAVITVPAYFNDAQRQATKDAGQI
                     AGLEVLRIINEPTAAALAYGLEKQDGKTIAVYDLGGGTFDISILEIGDGVFEVKSTNG
                     DTFLGGEDFDAKIVQFLADEFKKAEGIDLTKDKLALQRLKEAAEKAKIELSSAQTTEV
                     NLPFITADATGPKHLVKAIGRADLEKLVADLIKRTLEPCKKALADAGLKAGDIAEVIL
                     VGGMTRMPKVRDAVKDFFGKEPHTGVNPDEVVAMGAAIQAGVLQGDVKDVLLLDVTPL
                     SLGIETLGGVFTRMIDRNTTIPTKKSQTYSTADDNQNAVTIRVFQGEREMAADNKMLG
                     QFDLVGIPPAPRGVPQIEVTFDIDANGIVNVHAKDKGTGKEQQIRIQASGGLSDADID
                     KMVRDAEQFAEEDKKRRAAAEAKNNAESLIHTTERQLEEHGDKVEASLKSEIEAAVAE
                     ARTAVEGGDTDAMNTKAQALAQVAMKLGQAIYEKEQAAAASPDASAQGSEAGGGEEVV
                     DAEFSEVDDEAKG"
     gene            4906..6027
                     /gene="dnaJ"
                     /locus_tag="VF589_12215"
     CDS             4906..6027
                     /gene="dnaJ"
                     /locus_tag="VF589_12215"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8258387.1"
                     /translation="MNTDYYELLQVERTADEKAIKSAYRKLALECHPDRNPGCKDSEE
                     RFKAISQAYDCLKDPQKRAAYDRFGHAAFQNGGMGGGAQDFGSFSDIFENIFGEFMNG
                     AQGGRRSNVLRGSDLRYDLEIKLEDAYDGKEVELRIDTTVKCDPCAGTGAKPGTSVRQ
                     CTMCGGRGQVRASQGFFVVERTCPSCRGAGEVTADPCEHCRGEGRSEKRRSLQVRVPP
                     GVDEGTRIRLSGEGEAGVRGGPNGDLYIFIHIARHGLFQREGTTLFARCPVSFTTAAL
                     GGTIEVPGLDRQRHEIRIPPGIQSGKQIRQRGAGMPVLNGRGHGDMVIQVEVETPTRL
                     SSRQKELLEEFRKTETGDECPASSSFFRKLKGMWEGLTE"
     gene            6175..7107
                     /locus_tag="VF589_12220"
     CDS             6175..7107
                     /locus_tag="VF589_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8258388.1"
                     /translation="MRASFFAFASLVSFVTPTAAQPDFSKAELKVERIAPGVAVLIGP
                     GGNIGVSYGPGANAIIDDQYAPLTEKLIAAVASLDPDPIRFVVNTHWHGDHTGGNEAL
                     GKRGSVIVAHDNVRKRMSSEQFLSLFNEKVPASPKEALPVVTFDEGVTLHLNGDTLRV
                     IHVENAHTDGDSLVHWQKANVLHMGDTFFHRMSFPFIDLGSGGSIDGAIAAVDTALGI
                     ADDRTRIIPGHGPVATKAELAAYRAMLADIRARVAAGIAGGRTLEQIQAERPAARYGM
                     TDGFMKPDKFVETVHRSLQSPPAPESPGAKPHRH"
     gene            complement(7267..8580)
                     /gene="purB"
                     /locus_tag="VF589_12225"
     CDS             complement(7267..8580)
                     /gene="purB"
                     /locus_tag="VF589_12225"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX8258389.1"
                     /translation="MIPRYARAPMAAIWEPETRFRIWFEIEAHALDAMAELGVVPKEA
                     ARKVWDWWATGPSIDVAAIDGIEAVVKHDVIAFLTWVAEQVGPEARFLHQGMTSSDVL
                     DTCLAVQLKQASDLLLDDIDALLAVLERRAHEHKFTPTIGRSHGIHAEPVTFGLKLAE
                     AHAEFARNRERLVAARKDIATCAISGAVGTFANIDPSVEAHVAAKMGLEVEPVSTQVI
                     PRDRHAMFFATLGVVASSIERLATEIRHLQRTEVLEAEEFFAPGQKGSSAMPHKRNPV
                     LTENLTGLARMVRAYVTPALENVALWHERDISHSSVERYIGPDATITLDFALARLTGV
                     VDKLLVYPERMQKNLDRMGGLVHSQRVLLALTQAGASREEAYGLVQRSAMKVWQSDGE
                     LSLLDLLKADPEVAALLPEGKLEESFDLAYHLKHVDTIFGRVFGG"
     gene            8766..9755
                     /locus_tag="VF589_12230"
     CDS             8766..9755
                     /locus_tag="VF589_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258390.1"
                     /translation="MMKPILLASVFTLALPALAQTTGTTGATGTTGATGTAQTGNMEA
                     GTSQGTTDHSSHNMSTTQTSTSQTSTGTGTAGTGMAGDTGTTATSTSTSADAMSSTPQ
                     DDPGMAAAGQAGTTGQSGTGFTGTTSAQTTGQTDTGYSSAQTGTTSQTGTTGQTGTTG
                     QTGAMGETGATAQTGTTGTGMTDTGAAGASATAQTGTTGTGMTDTGTAGAGATAQTGT
                     TDATGAGWSGTTDTTGQHAAATAQTGSSTWASAGGQTFQPGNTNPERDARGISVISAP
                     AQVPAGWNGITGTAMGGPVADPATGETAGGDSYPACSATVTDNCLQTYERGRG"
     gene            10098..10331
                     /locus_tag="VF589_12235"
     CDS             10098..10331
                     /locus_tag="VF589_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258391.1"
                     /translation="MPRYFFALHNDVEADDEDGVELSDLAAARILALKTIAELIHDRV
                     AKGGRIDLTHSLEVLDEERRPVLLLPFRELVEP"
     gene            10402..10779
                     /locus_tag="VF589_12240"
     CDS             10402..10779
                     /locus_tag="VF589_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8258392.1"
                     /translation="MHALVIEDQYLIAMLIEDELRDLGYTSFDFVTREREAVEAAARR
                     CPDLITADNKLTDGTGVIAVQEICEKQVIPVVFIVAAPKSIQAPVPFAAVVAKPFEAR
                     ALRSAIGDALVLAREHRAAGMIG"
     gene            11088..11600
                     /locus_tag="VF589_12245"
     CDS             11088..11600
                     /locus_tag="VF589_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258393.1"
                     /translation="MKKIMMIAAALASIAATPALADDSAANAVYERMQQATEAADPAP
                     LLEQVYGPGATYLPRHKELGIDRREHVLKMLVGSQAHLRKSGGQLAIKFRVVERKRFG
                     DVYVDNGYMRTTVKPGKDAPEQVSYGKFVAVIARQPEGHWAFVTDADSDTPAANFEGA
                     KPVAGLKFDS"
     gene            complement(11616..12299)
                     /gene="radC"
                     /locus_tag="VF589_12250"
     CDS             complement(11616..12299)
                     /gene="radC"
                     /locus_tag="VF589_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX8258394.1"
                     /translation="MADASTDGSGHRARLRQRLFEGGPDALLDHELVEYLLALALPRR
                     DTKPLAKKLIREFGGFGALLSADAAAIARVGDVSEGAAAALKIAQASALRLLKSEVQD
                     RPILGSWQALLDYLHADLAHNPVERVRVLYLNAKNMLIRDEPMSEGSVDEAAVYVREI
                     IRRALEYHATALILVHNHPSGDPQPSQQDIRITRDVAEAGRHLRIGVHDHVIIGARGH
                     VSMRAMGLI"
     gene            12461..>13349
                     /locus_tag="VF589_12255"
     CDS             12461..>13349
                     /locus_tag="VF589_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exoprotein"
                     /protein_id="HEX8258395.1"
                     /translation="MTVAEDGTATRRALPRWRLLALGLLLLALLLVAALWTQRRQIAT
                     EYVNRELARRGVKATYKVTRVGFRTQRMEQLVIGDPRRPDATAEWVEIEISLGVRRPR
                     VELIRARGVRLNGRVVDGKVTLGELDKLLPPPTGKPFRLPNQRVDLADASIRLDTPAG
                     RIGIALEGSGNLAYSFEGRIAAVSRGLRAGDCRVTAPRLVADLRTEEERPTLTGPLRA
                     ESAACGGVDLIRPRLTLKATLDAGFDGGEGHADVELARLRSGGHAFDAISGRIGFDGD
                     ADGVRGGLDLAAARASVGDY"
BASE COUNT         2326 a         4551 c         4521 g         1951 t
ORIGIN      
        1 tggacggtga agctgttgag ccggtcgggt cgattgaggg tgaggcgggc aatgccggcc
       61 tccagcttga actcgatggt ttcgtaagcc atggccgccc ccatgaacgc agatgagatt
      121 gcggacaagg tggcgcgggc gatgctgtcg cgcgaaggca ccggccccgc ctgggggatc
      181 gcgatcgagg aggcgcgcga aggctatgcg cgggtgggca tgcggctccg ggcggacatg
      241 ctcaacggcc acggcatcgg ccatggcggc atggtgttcg cgctcgccga caccgccttc
      301 gcttatgcct gcaacagccg cgacgtgagg actgtcgccg cgcaggcctc gatcgtgttc
      361 ctcgatgccg cccgccagga cgagaggctg accgccgagg ccgaggagca ggcgcttgtc
      421 ggacgctcgg gcgtctacca tgtgcgggtc agcggcgagg atggccgcgc catcgccgag
      481 ttccaaggct atgcgcggag ccttggcggc cccgtcctca acagcggaga ttgaatgtga
      541 gcgagctgac ccaagccttt atctgcgatg cagttcgtac cccgatcggc cgctatggcg
      601 gggcgctcgc ccgcgtccgg gccgacgatc tggccgcgat gccgatcaag gcgctcatcg
      661 accgcaacgg cggcgtcgat tgggaggaga tagacgacgt catcctcggc tgcgccaacc
      721 aggcgggcga ggacaatcgc aacctcgccc ggatggcggg gctgctggcc gggctgcccc
      781 acaaatccgg cggtgtgacc ctcaaccggc tgtgcggatc ggggatggac gcggtcacca
      841 tggcggcgcg catcatccgc ggctccgagg ccgacctggt gatcgccggc ggctccgaga
      901 gcatgagccg cgcgcccttc gtcatggcca aggcaacgtc cgccttcgac cgcaatgccg
      961 agatgtacga caccacgatc ggctggcgct tcgtcaatcc caagatgaag ggtgcctatg
     1021 gcgatgacac catgccgtcg acggctgaga acgtggcgca ggaatggcag gtaagccgcg
     1081 aggatcagga tgctttcgcc gtccgcagcc aggagaaggc ttcagccgcc caggataatg
     1141 gccgcttcgc cgccgagatc gtcgcggtta cgatcccgca gcgcaagggc gacccgctgg
     1201 tggtggaccg cgacgagcat ccgcgcaaga ccagcgtcga agcgctggcc aagctgcgtc
     1261 cgatcgtgcg gcccgacggc acggtcacgg ccggcaatgc cagcggcgtc aacgacggcg
     1321 cggcggcgat gatcgtcgct tccgaggaag ccgcccgccg caacgggctg acgccacgcg
     1381 cccgcatcgt cggcggcgcc gtcgccggcg tcgagccgcg catcatgggt atcggccccg
     1441 cgccggcttc tcagaaactg ttgaagcgcc tgggtctaag tatcgcggac atggacataa
     1501 tcgagatcaa cgaggcgttc gcggctcaag ccctgccggt gctgcgcgag ctcgggctcc
     1561 ctgacgatgc cgctcacgtc aaccccaatg gcggcgcgat agcgctcggc catccgctcg
     1621 gaatgtccgg cgcgcgcctg gcgctcaccg ccacggagga actccaccgc accggcggcg
     1681 gctatgcgct gtgcgcgatg tgcatcggcg tcggccaggg catcgccatg gtgatcgagc
     1741 gggtctagcc gcgccggcgc caggggccga acgggaccag ggttcgatat tgctccagcg
     1801 gcggcgcggc ggacacggca tggccttcgc gcagcatgaa ggcgtgcttg acgatttccg
     1861 cttgttgctc aagtccgtaa agctcgaact tctgccccgg cttcagcgca taatcgtagc
     1921 ggcagaaggg atggcgcatc agcggcagat aataacggcc gcgggtctgc gcctgccaga
     1981 catgggtcat ctcgtgaatg aacaaggcct ggcgccaggg caggtcggcg gcgaaatccc
     2041 cgctatgctg gctgcccttg gggtggaagt ggatgtgccc gcacggcgcc atcgctacgt
     2101 tgcgaggctg gaagagaaac catttccgac ggtggatgcg gacgggggaa taatcgatcg
     2161 cgtcgccgaa cacggaggcg gcgagctcga cctccgcttg ggtgaggggg cggtcttcag
     2221 gccccggagc gacttgttct accacagccg aaacagcccc ttagggtagg gcgtcggatc
     2281 ggccgggccg accaccctgg cctcgaccgc gatctcccca atcgccctcc cttcggaatt
     2341 gccttcagcg aatcgaagct cggcgcggcc gccggggcgc agccgcggct cgagaccgaa
     2401 cagcatggca tgttttccgc ccggctcgaa cgagacttcg ctctgactgt gaagctccag
     2461 gtagggcatg gggcgcatgg ccgcgacgcc gtgcgcttcc atcgtctcgt gcagctccgc
     2521 ccgttcgaac ggtcccttga ccgcgacgag ccgcaagtcg ccggtgccaa cctcgatgtc
     2581 gaaataggcg gctgcgggcc gtcctgccac cgccggcaac cttatccagg cgttctttat
     2641 cgcggtaccc ttggagcagc cctggagcgc gggagcgagg gcgaacaaga gagccaacct
     2701 ccacaggctt ttcataacag ctccttccgc cgctgccctt agcgcaaggc cgcttcttgt
     2761 cgccaaaaaa tgcaccccta tatcgcttcc gaaacactcg cggtgcctca cgcatgggcc
     2821 gcggacagca tatattgggg cataagaggt acatatggct aaagttatcg gtatcgatct
     2881 cggcaccacc aacagctgcg ttgccgttat ggaaggcagc aagccgaagg tcgtggaaaa
     2941 tgcggagggc gcgcgtacca cgccgtccat cgtcgctttc gccaaggacg gcgagcggct
     3001 gatcggccag ccggccaagc gccaggcggt caccaacggc gacaacacga tcttcgcggt
     3061 caagcgcctg atcggccgcc ggttcgacga cccgattacc aagaaggaca ccgagctcgt
     3121 tccctacacc atcaccaagg ggccgaacgg cgacgcctgg gtgaaggcgg gcggacagga
     3181 ttactcgccg agccagattt cggccttcat cctccagaag atgaaggaaa ccgccgaggc
     3241 ctatctcggc gagacggtca cgcaggcggt catcaccgtc ccggcctatt tcaacgacgc
     3301 ccagcgccag gcgaccaagg acgccgggca gatcgccggc ctcgaagtgc tgcggatcat
     3361 caacgagccg accgcggccg cgctcgctta tgggctggag aagcaggacg gcaagacgat
     3421 cgccgtctac gaccttggcg gcggcacgtt cgacatctcg atcctcgaga tcggcgacgg
     3481 cgtgttcgaa gtgaaatcga ccaacggcga caccttcctc ggcggcgagg atttcgatgc
     3541 caagatcgtc cagttcctgg ccgacgagtt caagaaggct gaagggatcg acctcaccaa
     3601 agacaagctc gcgctgcagc ggctgaagga agccgccgag aaggccaaga tcgagctgtc
     3661 gtcggcgcag accaccgaag tgaacctgcc cttcatcacc gccgacgcga ccggacccaa
     3721 gcacctcgtc aaggcgatcg gccgcgccga cctggagaag ctggtcgccg acctcatcaa
     3781 gcggacgctc gagccgtgca agaaggcgct cgccgacgcg ggccttaagg cgggcgacat
     3841 cgccgaggtg atcctggtcg gcggcatgac ccgcatgccc aaggtccgcg acgccgtgaa
     3901 ggatttcttc ggcaaggagc cgcataccgg cgtcaacccg gacgaggtcg tcgcgatggg
     3961 cgccgccatc caggccggcg tgctgcaggg cgacgtcaag gacgtgctgc tgctcgacgt
     4021 gacgccgttg tcgctcggca tcgagacgct tggcggcgtc ttcacccgga tgatcgaccg
     4081 caacacgacc atcccgacca agaagtcgca gacctattcg accgcggacg acaatcagaa
     4141 tgcggtgacg atccgcgtct tccagggcga gcgcgagatg gcggcggaca acaaaatgct
     4201 cggccagttc gacctggtcg gcatcccgcc ggcgccgcgc ggcgtgccgc agatcgaggt
     4261 cactttcgac atcgacgcca atggcatcgt caacgtccat gccaaggaca agggcaccgg
     4321 caaggagcag cagatccgca tccaggcttc gggcggcctc agtgacgccg acatcgacaa
     4381 gatggtccgc gacgccgagc agttcgccga ggaggacaag aagcgccgcg cggccgccga
     4441 ggccaagaac aatgccgaga gcctgatcca taccaccgag cggcagctcg aggagcatgg
     4501 cgacaaggtc gaggcgtcgc tcaagtccga gatcgaggcc gccgtcgccg aggcgcggac
     4561 cgcggtcgag ggcggcgaca ccgacgccat gaataccaag gcccaggcgc tcgcgcaggt
     4621 cgcgatgaag ctcggccagg cgatttacga gaaggagcag gccgcggccg cgtctcccga
     4681 cgcctccgcc cagggcagcg aggccggcgg cggcgaggag gtggtcgatg ccgaattctc
     4741 cgaggtcgac gacgaagcca aagggtaagg catgactcct tccgttcgtc ctgagcctgt
     4801 cgaaggacgg tccttctcca accctgaggg gaagaagggc agggcttcga cggccccgga
     4861 tcaggtccgg ggccgaacgg ctggggggct cgggaacctc acgtgttgaa caccgattat
     4921 tacgagctgc tccaggtcga gcggaccgcc gatgaaaagg cgatcaagtc tgcctatcgc
     4981 aagctcgcgc tggaatgcca tccggaccgc aacccgggct gcaaggattc ggaggagcgc
     5041 ttcaaggcga tcagccaggc ttatgactgc ctgaaggacc cgcagaagcg cgccgcctat
     5101 gaccgcttcg gccacgccgc cttccagaat ggcggcatgg gcggcggagc gcaggatttc
     5161 ggttccttct ccgacatttt cgagaatatc ttcggcgagt tcatgaatgg cgcgcagggc
     5221 ggccggcgct cgaacgtgct ccgcggctcc gacctgcgct acgatctcga gatcaagctc
     5281 gaggacgcct atgacggcaa ggaagtcgaa ctgcgcatcg acacgaccgt caaatgcgat
     5341 ccctgtgccg gcaccggcgc caagcccggc accagcgtcc gccaatgcac gatgtgcggc
     5401 ggccgcggcc aggtgcgggc gagccagggc ttcttcgtgg tcgaacgcac ctgcccgagt
     5461 tgccgcggcg ccggcgaggt caccgccgat ccgtgcgaac attgccgcgg cgagggccgc
     5521 agcgagaagc ggcgctcgct ccaggtccga gtgccgcccg gggtcgacga gggcacccgc
     5581 atccggctgt ccggcgaggg cgaggcgggg gtccggggcg ggcccaatgg cgacctctac
     5641 atcttcatcc atatcgcccg ccacgggctg ttccagcgcg agggcacgac gctgttcgcg
     5701 cgctgcccgg tgagcttcac caccgccgcg ctcggcggca cgatcgaagt gccggggctc
     5761 gaccgccagc gccacgagat acggatcccg cccggcatcc agtccggcaa gcagatccgc
     5821 cagcgcggcg ccggcatgcc ggtgctgaac ggccgcggcc atggcgacat ggtgatccag
     5881 gtcgaggtcg agacgccgac ccggctctcc tcccggcaga aggagctgct cgaggaattc
     5941 cgcaagaccg agaccggcga cgaatgcccg gcctcctcga gcttcttcag gaagctcaaa
     6001 ggcatgtggg aagggctgac cgagtaaggc gctaccgggt gggctgctaa attcgtcatt
     6061 cccgcgaaag cgggaatccc acttcttccg gccctctcct cacccccgcg tcccgattcc
     6121 cgctttcgcg caaacgtcgc tatgctcgcc cccctgcttc accaggggga actcatgcgc
     6181 gccagcttct tcgccttcgc cagcctcgtc tcgttcgtga cgcccacggc cgcccagccg
     6241 gatttcagca aggccgagtt gaaggtcgag cggatcgcgc cgggcgtggc ggtgctgatc
     6301 gggcccggcg gcaatatcgg cgtctcctac ggccccggag ccaacgccat catcgacgac
     6361 caatatgcgc cgctcaccga gaagctgatc gccgccgtcg cgagcctcga ccccgatccg
     6421 atccgcttcg tcgtcaacac ccattggcat ggcgatcaca ccggcggcaa cgaggcgctc
     6481 ggcaagcgcg gcagcgtgat cgtcgcccac gacaatgtcc gcaagcgcat gtcgagcgag
     6541 cagttcctct cgctgttcaa tgaaaaggtg ccggcctcgc ccaaggaggc tttgccggtg
     6601 gtgacgttcg acgagggcgt gaccctgcac ctcaacggcg acactttgcg cgtcatccat
     6661 gtcgagaacg cccataccga cggcgattcg ctggtccact ggcaaaaggc gaacgtgctc
     6721 cacatgggcg acaccttctt ccaccgcatg tcgttcccgt tcatcgactt gggttcgggc
     6781 ggatcgatcg acggcgccat cgccgcggtc gacaccgcgc tcggcatcgc cgacgaccgg
     6841 acccggatca tcccgggcca cggcccggtc gccaccaagg cggagctcgc cgcctatcgc
     6901 gcgatgctcg ccgacatccg cgcccgcgtc gccgcgggca tcgccggcgg ccggacgctc
     6961 gagcagatcc aggcggaacg gccggccgcc cgctacggca tgaccgacgg cttcatgaaa
     7021 cccgacaagt tcgtcgaaac cgtccaccgc agcctgcaaa gcccgccggc gccggagagc
     7081 cccggcgcca agccgcaccg ccactgagcg cggctccccc tctcccgctc gcgggagagg
     7141 gcagggtgag ggtcttcttc ttcctcttct tgaactccgt cgccgcgccg cagcttcaag
     7201 aaaaaccctc atcctcccaa agctgacgct ttgggcgcct ccgtctcccg caagcgggag
     7261 aagggctcac ccgccgaaga cgcgcccgaa gatcgtgtcg acatgcttca ggtgataagc
     7321 caggtcgaag ctctcctcga gcttcccctc cggcagcagc gccgcaactt cgggatcggc
     7381 cttcaacaga tcgagcagcg acagctcgcc gtccgattgc cagaccttca tcgcgctgcg
     7441 ctggacgagg ccataggctt cctcgcggct ggcgccggcc tgggtgaggg ccagcagcac
     7501 gcgctgcgag tggacgaggc cgcccatccg gtcgagattc ttctgcatcc gctccggata
     7561 gacgaggagc ttgtcgacga cgccggtgag gcgggcgagg gcgaaatcga gggtgatcgt
     7621 cgcgtcgggc ccgatatagc gctcgaccga gctgtggctg atgtcgcgct cgtgccagag
     7681 cgcgacattt tcgagcgcgg gcgtgacata ggcgcggacc atccgcgcga ggccggtaag
     7741 gttctcggtc agcaccgggt tgcgcttgtg cggcatcgcc gacgagccct tctggccggg
     7801 cgcgaaaaat tcctcggcct cgagcacttc ggtgcgctgg aggtggcgga tctcggtcgc
     7861 gagccgctcg atcgacgagg cgacgacgcc gagcgtcgcg aagaacatgg cgtggcggtc
     7921 gcgggggatg acctgggtcg agaccggctc gacctccagg cccatcttgg cggcgacatg
     7981 ggcttcgacg ctggggtcga tattggcgaa ggtgccgacc gcgcccgaaa tcgcgcaggt
     8041 cgcgatatcc ttgcgcgccg cgacgaggcg ctcgcggttg cgggcgaact cggcatgggc
     8101 ctcggcgagc ttcaggccga aggtgaccgg ctcggcatga atgccgtggc tgcggccgat
     8161 cgttggcgtg aacttatgct cgtgcgcgcg ccgctccagc accgcgagca gtgcgtcgat
     8221 atcgtcgagc agcaagtccg acgcctgctt gagctgcacg gcgaggcagg tgtcgagcac
     8281 gtccgagctg gtcatgccct ggtggaggaa gcgcgcctcc ggcccgacct gctcggcgac
     8341 ccaggtcagg aaggcgatca cgtcatgctt caccaccgcc tcgatcccgt cgatcgcggc
     8401 gacgtcgatc gacggaccgg tcgcccacca gtcccagacc ttgcgggccg cctccttggg
     8461 aaccacgccg agctcggcca tcgcgtccag cgcgtgcgcc tcgatctcga accagatgcg
     8521 gaaccgggtc tcgggctccc agatggcggc catcggggcg cgcgcgtagc gggggatcat
     8581 ggcgatgtcc tggaattgga ggtggaggcg ccgcaattag caaagtcgtc ccgagccatc
     8641 aatcggacga acggcaggaa cgcccgcgcg tcgcggctcg ttcatgaccc cgagcctccg
     8701 gaccttccaa tccgtcgagg cccgcgtgtg ttttggcaca gtggaaaccg acaggagacg
     8761 atgacatgat gaagccaatc ctgctcgcga gcgtgttcac gcttgcgctc ccggccctcg
     8821 cccagacgac gggcacgacc ggcgccaccg gcacgacagg cgctaccggc accgcccaga
     8881 cgggcaatat ggaggccggc accagccagg gaacgaccga ccatagcagc cacaacatgt
     8941 cgaccacgca gacttcgacc agccagactt cgacgggtac cggcacggcc ggcaccggca
     9001 tggccggcga taccggcacc accgccacca gcaccagcac cagcgccgat gcgatgagca
     9061 gcacgccgca ggacgatccg ggcatggccg ccgccggtca ggcggggacg acgggccagt
     9121 ccggcaccgg cttcaccggc accaccagcg cgcagacgac cggccagacc gacaccggct
     9181 atagcagcgc ccagaccgga accacgagcc agaccggaac cacgggccag accggaacca
     9241 cgggtcagac cggcgcgatg ggcgagaccg gcgccaccgc ccagaccggg accaccggca
     9301 ccggcatgac cgacaccggc gcggccggcg ccagcgccac cgcccagacc ggaaccaccg
     9361 gcaccggcat gaccgacacc ggcacggccg gcgccggcgc cacggcgcag accggcacca
     9421 ccgacgccac cggcgcgggc tggtcgggca ccaccgatac gaccggccag catgcggccg
     9481 ccaccgcgca gacgggcagc tcgacctggg cgtcggccgg cgggcagacc ttccagcccg
     9541 gcaacaccaa tcccgagcgc gacgcgcgcg gcatcagcgt gatctccgcc ccggcgcagg
     9601 tcccggccgg ctggaacggc atcaccggca ccgcgatggg cggcccggtc gccgaccccg
     9661 ccaccggcga gaccgccggc ggcgacagct atccggcctg ttcggcaacc gtcaccgaca
     9721 attgcctcca gacctacgag cgcggccgcg gctaaggccg ggacgagcga gtaccgaaag
     9781 ggaagggcgg ctccggccgc cctttctttt gcggtttttt gcgtcggagc agcaccatga
     9841 cggacacgga ccctctggac caggacgacg ctcgcccgcc ccgcccggcg actcacccgg
     9901 ccccgcacga gccgcatggc ggaagccccg gctcgaagcc tgagcccggc gacggctcgc
     9961 cggaagagaa gacgatgcgc gagccggact aggcggcgcc tgtcccgttc ggcggggcgg
    10021 gcggagagcc ggacgaatgt cgttacgggt ctaatcgagg ttgtggaccg gcgcgacaaa
    10081 cccgctatgc ggcgcccatg ccccgctatt ttttcgcctt gcacaatgat gtcgaagcgg
    10141 acgacgaaga cggcgtggag ctgtcggacc tcgcagccgc gcgcatcctc gccctcaaga
    10201 ccatcgccga gctgatccac gacagggtcg ccaagggcgg caggatcgac ctcacccaca
    10261 gcctcgaggt tctggacgag gagcgcaggc ccgtgctgct gctcccgttc cgggagctgg
    10321 tcgagccctg aggcagaacg cccggccatc gactctcgtt acgggcgcgc ctagaagccc
    10381 ctcacaaggg ggcactcgcc catgcacgcg ctcgtgatcg aagaccaata tctgatcgcg
    10441 atgctcatcg aggacgagct gcgcgacctc ggctacacgt cgttcgattt cgtcactcgc
    10501 gaacgcgaag cggtcgaagc ggccgcgcgc cgctgccccg acctcatcac cgccgacaac
    10561 aagctcaccg acggcaccgg agtgatcgcg gtccaggaga tttgcgagaa gcaggtgatc
    10621 ccggtcgtgt tcatcgtggc cgctcccaag tcgatccagg cgccggtccc gttcgcggcc
    10681 gtcgtcgcca agccgttcga agcgcgggcg ctccgcagcg ccatcggcga cgcgctcgtc
    10741 ctggcgcgcg agcaccgcgc cgccggcatg atcggatagg agaggccgcc gcccttcccc
    10801 ctctcccgct cgcgggagag ggtagggtga gggtcttctt cttcttcttc ttcttcctta
    10861 agcacggtgg cagcgccgca gcttcaagaa agaccctcat cctcccaaag ctgacgcttg
    10921 ggcccctcct tctccctcaa gcgggagaag ggctggtccg cagcgccgcc ctcgcgcttg
    10981 gcgcagccga ttcgtagggc ggattgggcc gttcgtcgaa aagcgccttc ccggccgcca
    11041 tcccttgcta gcccttcgcc accacaaccg accgtctggg ggcaaggatg aagaagatta
    11101 tgatgatcgc cgcggcgctg gcctcgatcg ccgcgacgcc ggcgctggct gacgacagcg
    11161 ccgccaacgc ggtctacgag cgcatgcagc aggccaccga ggcggccgac ccggcgccgt
    11221 tgctcgagca ggtgtacggg cccggcgcga cctatttgcc gcggcacaag gagctcggca
    11281 tcgaccggcg cgagcacgtc ctcaagatgc tggttggttc ccaggcgcat ctgcgcaaga
    11341 gcggcggtca acttgccatc aagttccgcg tggtcgagcg caagcgtttc ggcgacgtct
    11401 atgtcgacaa cggctacatg cgcacgacgg tgaagccggg caaggacgcg cccgagcagg
    11461 tctcctacgg caagttcgtg gcggtgatcg cgcggcagcc ggaaggccat tgggcgttcg
    11521 tcaccgatgc cgattcggac acgcccgccg ccaatttcga gggcgccaag ccggtcgcgg
    11581 gcctgaagtt cgacagctga acggcggggg agggctcaga tcagccccat cgcccgcatg
    11641 ctgacatggc cgcgggcgcc gatgatgaca tggtcgtgga cgccgatccg aaggtgacgc
    11701 ccggcctcgg ccacgtcgcg ggtgatcctg atgtcctgct ggctcggctg cggatcgccg
    11761 ctcggatggt tgtggacgag gatcaaggcg gtcgcgtgat attcgagcgc gcggcggatg
    11821 atctcgcgga cgtagacggc ggcctcgtcg accgaaccct ccgacatcgg ctcgtcgcgg
    11881 atcagcatgt tcttggcgtt gagatagagc acccgcacgc gctcgaccgg attgtgggcg
    11941 agatcggcgt ggaggtaatc gagcagggct tgccagctgc ccaggatcgg ccggtcctgc
    12001 acttccgact tcaatagccg cagcgccgac gcctgggcga tcttgagcgc ggcggcggcg
    12061 ccttcggaga cgtcgccgac ccgcgcgatc gccgcggcgt cggccgacag cagggcgccg
    12121 aagccgccga actcgcggat cagcttcttg gcgagcggct tggtgtcgcg gcgcggcaag
    12181 gcgagcgcca gcagatattc gaccagctcg tgatcgagca gcgcgtcggg cccgccctcg
    12241 aacaggcgct ggcgcaggcg cgcgcggtgg ccgctcccgt cggtggatgc atccgccata
    12301 agccaggatt aagcttcctc ctgtccatag gcaatcgagg gcgatatgcg tgtcgcttgc
    12361 cccgcgccgg actgccggcg gtggaacgtg ctgcgttgat ggaggtaagg gccgacgtgc
    12421 ctcggacacg ggtttgcgac agtcacggca tgatccgtcc gtgacggtcg ctgaggacgg
    12481 aacggcgacg cggcgcgcgc tgccgcggtg gcggctgctg gcgctcgggc tgctgctgct
    12541 ggcgctgctg ctggtggcgg ccttgtggac gcagcgccgg cagatcgcga ccgaatatgt
    12601 caatcgcgag ctcgcccggc gcggcgtcaa ggcgacctac aaggtgacgc gggtcggctt
    12661 ccggacgcag cgtatggagc aattggtgat cggcgacccg cgccggccgg acgccaccgc
    12721 ggagtgggtg gagatcgaga tttcgctggg ggtgcggcgg ccgcgggtcg agctgatccg
    12781 cgcgcgcggc gtccggctca acgggcgggt ggtcgacggt aaggtgacgc tcggcgagct
    12841 cgacaagctg ctgccgccgc cgaccggcaa gcccttccgg ctgcccaacc agcgggtcga
    12901 cctcgccgac gcctcgatcc ggctcgacac gcccgccggc cggatcggca tcgcgctcga
    12961 gggcagcggc aatctcgctt atagtttcga agggcggatc gcggcggtgt cgcgggggct
    13021 tcgcgccggc gactgccggg tgacggcgcc gcggctggtc gcggaccttc gcaccgagga
    13081 ggagcggccg acgctgaccg gcccgctacg ggcggagagc gcggcctgcg gcggggtcga
    13141 tctcatccgg ccccggctga cgctcaaggc gacgctcgat gccgggttcg acgggggcga
    13201 gggccatgcc gatgtcgagc tggcgcggct gcgctccggc ggccatgctt tcgacgccat
    13261 cagcggccgg atcggcttcg acggcgatgc cgacggcgtg cgcggcgggc tcgacctcgc
    13321 cgccgcgcgc gcttcggtcg gcgactatc
//
LOCUS       DASVRK010000293        17577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_370812, whole genome shotgun sequence.
ACCESSION   DASVRK010000293 DASVRK010000000
VERSION     DASVRK010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 17577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17577
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370812"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VF589_12260"
     CDS             complement(<1..1000)
                     /locus_tag="VF589_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HEX8258396.1"
                     /translation="MRDHSLSRIIALARAGSTSRAWREFVAAGHDADTGDAAALAVKG
                     RLLKDRALAAAGADRNAFYAAAADAYRQAAALTRSSYHGINAATLSLLGGEPDAAQLV
                     AGEVLELLRDQPEEGETPYWRVATRAEALLLLGRDYEASTSFVEAIALAPEAWEDHAS
                     TLRQFALILDAQGKDSAWLDAHRPPRSLHFGGHMSFDAGVSRREHLDRKIAALLEEEK
                     VGFGYGALAAGADIIVAEALVARGAELHAVLPGGAEAFAAVSVDPFGAVWRRRFDALL
                     DRAATVRAVRPLGAPPDPETIALADEIAMGAAAMNARRLESQALQLLVADADAGERE"
     gene            complement(1052..1564)
                     /locus_tag="VF589_12265"
     CDS             complement(1052..1564)
                     /locus_tag="VF589_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258397.1"
                     /translation="MHRKLTFDCSGMTFDYSVVDMPTNNQDNKFGDIGVFCRSIVRKD
                     HVLKGPDAREDKRLDVPISKHSYVIFELPEEWTFSSKADRPGVTLGIKPGEGNPDSDA
                     YGQLRYVTASTVSPAPIEKCRLVFFSAIDRDGTVANPYIQPINFNIVTSDGVAVVVDP
                     DIRFPGNGTS"
     gene            complement(1654..3198)
                     /locus_tag="VF589_12270"
     CDS             complement(1654..3198)
                     /locus_tag="VF589_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8258398.1"
                     /translation="MGSGRYTAFLSYSHRDADQARWLHRRLEAYRLPRRLVGTEGDHG
                     PVPARLAPIFRDREELPAAGDLSETVRAALAVSDHLIVVCSPNSAASPWVAREIAVFR
                     ELHPGRPILAAIVDGEPTQCFPAGLADGTEPLAADLRPGHDGRRLGFLKLVAGLAGVG
                     LDALIQRDAARRLRRVTYVTAGAVAAMLVMAIMTTLALTARAEAQRQRAEAEGLVEFM
                     LTDLRDKLKGVKRLDVFAAVNERALRHYSRQDLQDLPVDALERRARLLHAMGEDDLAR
                     GHSERALLQFREAARTTASLLAGDPQNPDRVFAHAQSEFWLGSIHYQSGRPMLARISF
                     QKYKDLAERLVEMGPGNADWLKEAGYANGSLCSLYLEEPFEPSAALALCSEALSRMER
                     AHHLTGSTPSTLEAILNRHLWMMKAWDANGRWDRAVYHKQRQETLMEALLRADPANMD
                     YQDIWMRAQVGIAELLISHGERAEARQRLGLAAATANRLRKHDPANADWRSWQQKIQS
                     QLKQGE"
     gene            complement(3478..3864)
                     /locus_tag="VF589_12275"
     CDS             complement(3478..3864)
                     /locus_tag="VF589_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8258399.1"
                     /translation="MPAPYPVYVVDDDPEVREWILLLCQDQGMAGTGFAGGEAFLEAA
                     GGLAPGCVLLDMRMPGLSGLQVQAELSRRGLKMAVVAMTGYGGVATAVRSMKLGAVDF
                     LEKPFGADALLAALAAAGARLDQSRG"
     gene            4090..4560
                     /locus_tag="VF589_12280"
     CDS             4090..4560
                     /locus_tag="VF589_12280"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEX8258400.1"
                     /translation="MAKRERRVIGWREWVELPDLLAMPIKAKIDSGARTSSLHSFGTR
                     RFSEGGAPWVEFLLHPLQRQARPEIACRAAIKDERVVRSSNGEAEMRIVVETLARLGD
                     TQWPIELTLAARDVMGFRMLLGREAIRRRFLIDPARSFRQSTRPGQPPKKKKPK"
     gene            4557..5462
                     /gene="rimK"
                     /locus_tag="VF589_12285"
     CDS             4557..5462
                     /gene="rimK"
                     /locus_tag="VF589_12285"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEX8258401.1"
                     /translation="MKIALLCRSPQLYSHRRIMEAARARGHDIDAINHLRCTIDIASH
                     RPKIHYQGRVLAGYDAVIPRIGASVTFFGTAVVRQFEMMRVYSVNESVAIARSRDKLR
                     SLQLLSRRGIGLPVTVFAHKTSNAEDIVRLAGGAPVVIKLLEGTQGIGVILGETPKAA
                     ESIIQAFGGVNTNILVQEYVQEAHGEDIRCLVIGGKVVASMIRRGREGEFRSNLHRGG
                     SARAVRITPQERATAIGAARAMGLNVCGVDMLRSNHGPVVMEVNSSPGLEGVEQATGV
                     DVAGKIVQFIEKNAEPGKTRTRGKG"
     gene            5463..6500
                     /locus_tag="VF589_12290"
     CDS             5463..6500
                     /locus_tag="VF589_12290"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX8258402.1"
                     /translation="MARRPFSFGGEEVPAGGRRLVSLPMSVLSNRTPMALPVSVIHGR
                     REGPTLFVSAALHGDEVNGVEICRRLLASKALAVARGTLLVVPIVNNFGFISHSRYLP
                     DRRDLNRSFPGSARGSLAAQLAYALMTEVVARSQFGIDLHSATIHRSNLPQIRVDSGY
                     PAGLELARAFGAPIIVHSPTRDGSLRKTAQERGVPVIVYEGGEGLRADEFSVSVGMRG
                     VLRVLDHLGMVRKRTAGRLRPPALAMSSRWVRAEESGLFRPLKEIGDRVAAGERIGIV
                     ADPYGEMEAEVTAGSAGIVIGRANNPAVNQGDALFHVAEVEDPTRLEQHYGTLSRELQ
                     SERVLDEDEIL"
     gene            complement(6497..7282)
                     /locus_tag="VF589_12295"
     CDS             complement(6497..7282)
                     /locus_tag="VF589_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8258403.1"
                     /translation="MVDLAPLTSVEIPSLKGKVAEEEWKVRVDLAAAYRLVAHYGWDD
                     LVFTHLSARIPGPEHHFLLNPYNLMFEEVTASSLVKVDMDGNPVDATPFITNPAGFTI
                     HSALHMARPDAQAVMHLHTPHGQAVAAHAEGLLPLTQTAMLIRGDLAYHDYEGVAVDL
                     DERERLVANIGSKNAMLLRNHGTLAVGGNVGEAFIRLYFLERACQAQILALSAGKDVN
                     NPPQGTPEVAAEQGAMGLKLAANFLAWPALLRKMYRLDPSLAT"
     gene            complement(7345..7986)
                     /gene="maiA"
                     /locus_tag="VF589_12300"
     CDS             complement(7345..7986)
                     /gene="maiA"
                     /locus_tag="VF589_12300"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX8258404.1"
                     /translation="MTRPVLYDYFRSSAGYRVRIALNLKRIEHDRVPINLLEGAQKEP
                     GYTGRNPQGLVPTLEIGGLRLTQSLAIIDYLDALEPEPPLLPRDPADRAHVLALALVV
                     ACDTHPINNLRVLKYLSGPLGLDPETRSAWYAHWVIEGLAALEAMAAPRAGRFLFGDA
                     PSLADICLVPQLYNARRFDIPLGDFPRLVSVEAEAGRIEAFAAAHPDVVAASE"
     gene            complement(8051..8533)
                     /locus_tag="VF589_12305"
     CDS             complement(8051..8533)
                     /locus_tag="VF589_12305"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HEX8258405.1"
                     /translation="MRKAAALLAGLAVLAAVNFGIYQRERLLADGRVVLLKLSPIDPR
                     SLMQGDYMRLNFEVADQAFPGFSDTEMGDGHIVVGLDARNIAHFRRFAGAGPLAPNEI
                     ALRYRIRGGEPNFATNAYFFQEGRAAAYEKALYGEFRVGSGGDMILTRLRGPRLEVLD
                     "
     gene            complement(8526..9596)
                     /locus_tag="VF589_12310"
     CDS             complement(8526..9596)
                     /locus_tag="VF589_12310"
                     /inference="COORDINATES: protein motif:HMM:NF025709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HEX8258406.1"
                     /translation="MTGEALWVRLGAENLVEGGYPARGQPQSPWYVRAMLGFAGWIGA
                     LFLIGFVGAAFASVLDDGPSATIAGAACCGAAFALFRSFDEQVFVEQFALALSLAGQM
                     LLVVGLAQLFQPDRPGFYLAVAATQAALALAVPNFLHRVLATTGAAIALALAVHRLSL
                     HGFAAPLLCVSVAWLWLQPTRWAGSGGLWRPVGYGLVLALLLVETFRLFGVETWFERG
                     DEAPSWIMVHGPAIGQALAGAALVWVAVALSRREGFAPASRTALSAAGAALLIGLLSL
                     SAPGLASALLILLLGFAAGNRILMALGILSLLGFVAHFYYSLHATLIEKAAILAVTGL
                     CLLAAHLFLRRGAAGAEQLGHA"
     gene            complement(9593..10546)
                     /locus_tag="VF589_12315"
     CDS             complement(9593..10546)
                     /locus_tag="VF589_12315"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEX8258407.1"
                     /translation="MSRLRSEVFEWLDAGRVEPGREGEALRAAGVIPTPVDWRTFLGQ
                     LSLWLGTVALAASVIFFFAFNWDDLGRFAKFGLVEAAICAGLLVCCWTDLDGMGGKAI
                     LLLLSLLVGALLALAGQVYQTGADTFELFGWWAVLILPWVLIARFSPLWLLWLVLLNL
                     AASFYFSLFSDGEELAWVLFAVNGLALAAWEAGRRLGLSWLRDSWTPRLVALASAGTA
                     TALISWAILDAENGRGVLAAVAYLAWLGGLYAWYRHARPDLFMLAAGLLSLIVTVAVF
                     LSRQMLGSDDGASFLLIGLVVIGMAAGGAVWLKSVAREQPA"
     gene            complement(10646..12784)
                     /locus_tag="VF589_12320"
     CDS             complement(10646..12784)
                     /locus_tag="VF589_12320"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8258408.1"
                     /translation="MSRILYFALELLIVVVAVLLGAEMAQRVHLVPRSILDIPDVYFR
                     ASDIRTVLVAFLIACTAAFLANRFISGWDPFESPRRAAKEIYAYLTGAVASALYLFFL
                     TPINFSPEFLLDAFLIGFALLLVAFLAIRWIRGRQAPLGQLGGLVGSLFGLLKSPFIW
                     LVILFAMTPLAAAYKFTTDRDFGNWVTNLRISSNDDGDYPYAVVSAIGDTRFETPIMA
                     QFADGDPRHIYVLERNGRLYRADYPSGGNKLLLLDLRDGVGYVEMENGALGFDLHPEF
                     GRAGSPNAGFVYVFFTEYHRDRQINRLSRYDISLPDTAARTASGLPLIAQGRSSNGYH
                     NGGSVEFGPDGFLYLAVGESDMEVCHQRLECALVAGILRIDVDQKGGTISRPIGRQPE
                     NGTTAHYYIPLDNPYANRPELLGEYWAHGLRNPFRIAFDRGTGKLWAGDVGSAMWEEV
                     NLLERGGNYQYPYIEGREVQKEYPRPARIDGRERPPLLTYHHTALLRSVIGGLVYRGN
                     RLPEAQGRYVFLDNYSGQVMTMPATGASVEDFDIIARTPEVSQRGPTGFAAAPDGELL
                     VVMMGDNDQPTGMIGKLVPAGSASARTAAAEQAATKAAAEKQGVTAAQASNLFNTNCG
                     RCHGTGGKGDGPDAKLIEGGVPDFTRADFQAARNDQRLRTAIKAGGPAVGGSEYMPPW
                     EGTLTEPEIDAMVAHVRGFKGKNAPAQAGQ"
     gene            complement(12948..14855)
                     /locus_tag="VF589_12325"
     CDS             complement(12948..14855)
                     /locus_tag="VF589_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8258409.1"
                     /translation="MSTRFSRPLRGGLPAAALAAAGVLAAPVSAQEAEAIVVTGSRTG
                     HAAEGFDTATISAPEIELLQPANVLDLLGRVPGVRAFGKGGAAGPSYLSVRGGEPNFT
                     LVLIDGVKVTDPTNSRGGAFDFGQIDPLALDRIDVAKGGLSAVHGADALSGVVNLRLR
                     TIGAGERLASARAAADSEGGYAVTALGGYGWESGALLGGVSAADSGDLTEGSDIERDQ
                     LFARLTQQVGAVSLSALALHARADREVFPEDSGGPRLAVNRERERRETALSLAGLTLA
                     GPADGAWQPRLALSGSRQRDDADTPAIAPGPVLDGVPAIRSDSRFRRAEAVFDNRLRL
                     GEAAELAFGAAWLGERGVSEGTIDFGILIPADFRIERSIASAFAEATLRPAERLTATF
                     GIRHDDPSTAAAEWTGRASARWQPLAGGPTLVGSWSEGYKLPSLYALAYPIIANPDLR
                     PERSRNFDFGLEQAWADGRGLARIGYFHSRYSDLIDFDAETFTNVNRSRVTAQGVEAE
                     IRAPLAATLAVQAALTYLDTDQPADAPPLRSRPEWSGLVALDWRPDPRLRLEASAAYT
                     GDYFDSSVPTGLVTLEPRLVVSAAAQYRLSEAVALTLPGENLLSRDYEETIGFPAPAR
                     VIRLGLLFRPL"
     gene            complement(14866..16029)
                     /locus_tag="VF589_12330"
     CDS             complement(14866..16029)
                     /locus_tag="VF589_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8258410.1"
                     /translation="MAEAYIVEAVRTAGGKRGGALAGWHPADMAAEVLNALVDRSGVD
                     PAAIDDVVMGCVGQAGEQAFHVGRNAVLASKLPQSVPAVTIDRQCGSSQQSIHFAAQA
                     VMSGTQDLVIAAGVESMSRVPMGLPFTLPMQHGIGTGPFSQRIQERFGVAMFSQFHGA
                     EMIAEKYGFSRDALDAFALDSHIKAARATQAGAFAGEIVPLATEAGEHRADEGIRYDA
                     TIEGIASVKLLREGGRISAANASQICDGASGVMVVSEQALKDHGLTPLARIHNLTVTA
                     GDPVIMLEEPIPATRRALGRAGLGIADIDLYEVNEAFASVPLAWLKETGADPDRLNVN
                     GGAIALGHPLGASGAKLMATLVHALRARGKRWGLQTMCEGGGIANVTIVEALA"
     gene            complement(16102..16174)
                     /locus_tag="VF589_12335"
     tRNA            complement(16102..16174)
                     /locus_tag="VF589_12335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16139..16141),aa:Thr,seq:cgt)
     gene            16340..>17577
                     /locus_tag="VF589_12340"
     CDS             16340..>17577
                     /locus_tag="VF589_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258411.1"
                     /translation="MDSFRGFDDSAEADVLGEDEPSIERPPEIGVDERRMHVRAYNYW
                     VSLLDGRAYPSIRDLDPGNLDDFGPYSVLLNFSSDADNPEVAFIGRALRQETGLGDDI
                     KTISDVPSRSLLSRLTDHYMQIIANCAPIGFEAEFVGQSGHQTMYRGILMPLSSDGDT
                     IDFIYGVINWKEVADGKTAAALAREVSRAAAAPAIEASPVWADGPNAEPLDEAFSALP
                     REASYELAVDDDQPSLDLPLAADAALADRLCAARDCAEAVKSADARSRSALYRALGHA
                     YDFALAAEASPEDYAELLEDAGLKAQARAPMTPIAKLVFGAGYDKTRLTEFAAALSCA
                     RREGVAQSGFVAWLEGFGGGLKGAVGAERLARRPAAKPDRFEAVRAALRQVVPVAHVS
                     VPSEVQEGDFVLLVARREADG"
BASE COUNT         2902 a         5893 c         6107 g         2675 t
ORIGIN      
        1 gctcgcgctc gccggcgtcc gcgtcggcga cgagcagctg cagcgcctgg ctttcgagcc
       61 ggcgcgcgtt catcgccgcc gcgcccatcg cgatctcgtc ggcgagcgcg atcgtctccg
      121 ggtcgggcgg cgcgccgagc ggccgcaccg cccgcaccgt cgccgcccga tcgagcaagg
      181 catcgaagcg ccggcgccac accgccccga acggatcgac ggaaacggcc gcgaacgcct
      241 cggcgccgcc gggaagcacc gcgtgcagct cggcgccgcg cgcgaccagc gcctcggcga
      301 cgatgatgtc ggcgccggcg gcgagcgccc cgtaaccgaa cccaaccttc tcctcctcga
      361 gcaaggccgc gatcttgcgg tcgagatgct cccgccgcga cacgcccgca tcgaacgaca
      421 tgtgcccgcc gaaatggagc gagcgcggcg ggcggtgggc atcgagccag gcggaatcct
      481 tcccctgcgc gtcaaggatc agcgcgaact ggcgcagtgt cgaggcatgg tcctcccacg
      541 cctcgggggc gagggcgatc gcttcgacga agcttgtact ggcctcatag tctcgcccga
      601 gcaggagtag agcttcagca cgcgtcgcga ccctccaata gggcgtttcg ccctcttcag
      661 gttgatcgcg aagcagctca agcacctcac cggccaccag ctgcgccgcg tcaggctctc
      721 cgcccagaag ggacagggtt gccgcgttta tcccgtgata gctggatctg gttaacgcgg
      781 ccgcctgccg ataagcgtcg gcggcggccg catagaaggc attgcgatct gccccggccg
      841 cggccaatgc ccgatccttg agaaggcgcc cttttactgc aagggcggcc gcatccccgg
      901 tatcagcatc atgaccggcc gcgacgaact cgcgccacgc cctcgaagtc gatccggctc
      961 gagccagtgc gatgatccgc gaaagtgagt ggtcccgcat tgaatccccg gaagctgcgc
     1021 caaacgggaa cggctatctt cacccgtacc ctcacgaggt cccattgccg ggaaaccgta
     1081 tatccgggtc taccacaacc gcaacaccat ctgacgtgac gatgttgaag ttgatgggct
     1141 gtatataagg attcgccacg gttccgtcgc gatcgattgc agagaagaaa accaaccggc
     1201 atttctcaat cggcgccggc gagaccgtgc tggccgtgac ataacgtaat tgcccgtaag
     1261 catcgctgtc cgggttgccc tctcccggtt tgataccgag cgtcaccccc gggcggtctg
     1321 ccttcgacga gaaagtccac tcttcaggaa gttcaaatat aacatatgaa tgcttgctta
     1381 tcggtacatc gagacgtttg tcctcgcgag catccggtcc cttaagcaca tgatcttttc
     1441 tcactattga acgacaaaaa acaccgatat ccccaaactt gttatcctga ttattggtcg
     1501 gcatatcgac gactgaataa tcaaatgtca taccactgca gtcgaaggtc agtttgcggt
     1561 gcacgtattg ttccgggtta aacccgggaa cagggcggtc ctggattaaa acttggtttt
     1621 cagaacgtga tataattatt gacggctgca tcgctattct ccctgcttca gttggctttg
     1681 gatcttttgc tgccagctac gccaatccgc gtttgcgggg tcatgtttgc gcagccggtt
     1741 tgccgtagcg gcggctaatc caagtcgctg tcgggcttca gcacgctcgc cgtgagagat
     1801 cagaagctcg gctatgccga cttgggctct catccagatg tcctggtaat ccatgtttgc
     1861 agggtcggcg cggagcaatg cctccatcag cgtttcctgg cgctgcttgt ggtaaactgc
     1921 cctgtcccac cggccattgg cgtcccacgc cttcatcatc cagaggtgcc ggttcagaat
     1981 ggcctccagg gtggacggcg tgctgccggt caagtggtga gcccgctcca tgcgagagag
     2041 cgcctcggag cagagtgcaa gcgccgccga tggctcgaaa ggctcctcca aatatagcga
     2101 gcacaggctg ccgttcgcgt atcctgcttc cttgagccaa tctgcgttgc ctggccccat
     2161 ctcgacgaga cgttcggcca ggtctttgta tttttgaaat gagatccggg cgagcatcgg
     2221 tctgccgctc tgatagtgaa tgctgccgag ccagaattca ctttgcgcgt gcgcgaaaac
     2281 tcgatccggg ttctggggat cgccggcaag caaggaagca gtcgtgcgag ccgcttccct
     2341 gaactgcaac aatgcgcgct cactgtggcc gcgggcgagg tcatcttcgc ccattgcgtg
     2401 cagaaggcgc gcacgtcgct ccagcgcgtc gaccggcaga tcctgaagat cctgccgtga
     2461 ataatggcgg agcgcgcgct cgttgaccgc cgcgaaaacg tcgagccgct tcactccttt
     2521 aagcttgtcc cgcaaatcgg tcagcatgaa ctcgaccagc ccttcggcct cggcgcgctg
     2581 gcgttgcgct tcggcgcggg cggtgagggc cagggtggtc atgatcgcca tcactagcat
     2641 cgccgccacc gcccccgccg tgacgtaggt cacgcggcgc aggcgtctgg cggcgtcgcg
     2701 ctggatgagg gcgtcgaggc cgacgccggc cagcccggcg accagcttga ggaagccgag
     2761 gcggcggccg tcgtggccgg ggcggaggtc ggcggcgagg ggttcggtgc cgtccgccag
     2821 accggccggg aagcattggg tgggctcgcc gtcgacgatc gcggccagga tcgggcggcc
     2881 ggggtggagc tcgcggaaga cggcgatctc ccgggccacc cagggggagg ccgcgctgtt
     2941 gggcgaacag acgacgatca gatggtcgga gacggcgagc gcggcgcgga cggtctcgct
     3001 gagatcgccc gcggccggca gctcctcgcg gtcgcggaag atcggcgcca accgcgcggg
     3061 gacggggccg tgatcgccct cggtcccgac caggcggcgc ggcaggcgat aggcttcgag
     3121 ccgccggtgc agccagcgcg cctggtcggc gtcgcgatgg ctgtagctta agaatgcggt
     3181 gtagcgccca ctccccacgg tcgcgtctcc cctggctcgg tccggagccg agacggagcc
     3241 tactccaagt tagggaaaga ggaagccatt gccggtccga acgccgctgt aaggccagca
     3301 ataattcaat ccggtttgga gattaggttg gatatttgcg acggtcggca cggcccgatg
     3361 ctgccgaacg accgaccaac caacggtgat ttaggtaacc gtccggcggc tcgggatccg
     3421 gtttcgggca ggttgctgtc cttgggcttt gaccattgcc aattcagtgc gcgatcctca
     3481 gccccggctc tggtcgagcc gcgcgccggc ggcggcgagg gcggcgagaa gggcgtcggc
     3541 gccgaacggc ttttcgagga aatcgaccgc gccgagcttc atcgagcgga ccgcggtggc
     3601 gacgccgcca tagccggtca tcgccaccac cgccattttg aggccgcggc ggctgagctc
     3661 ggcctggacc tggaggccgc tgaggccggg catccgcatg tcgagcagca cgcagccggg
     3721 cgcgagcccg ccggccgcct caaggaacgc ctcgcccccg gcaaagccgg tgccggccat
     3781 gccctggtct tggcagagca gcaggatcca ttcccgcacc tccgggtcgt cgtcgaccac
     3841 gtagaccggg taaggcgcgg gcatggcggc ggaaatcctt tcgaggggcg tcaacaggcg
     3901 aagccagggg cgccgaccag agccccctaa aagccggctg cgctttcgga cagggaaata
     3961 tgtccggata gcgccgcttt caccatttcg gcgcagcttt ccggcagctc tccgcgccgg
     4021 gcggggccgc ctttccaatc gcccgccgcg cctgataagg acgaggcggc gacggggcgg
     4081 ggatcatctg tggcgaagcg cgaacggcgg gtgatcggat ggcgcgaatg ggtcgagctt
     4141 cccgatctgc tcgccatgcc gatcaaggcc aagatcgaca gcggcgcccg cacctcctcg
     4201 ctgcacagct tcggcacccg ccgcttttcc gaaggcggcg cgccgtgggt ggagttcctg
     4261 ctccatccgc tccagcgcca ggcccgcccc gaaatcgcct gccgggcggc gatcaaggac
     4321 gagcgcgtgg tcaggagctc gaacggcgag gcggaaatgc ggatcgtcgt cgagacgctg
     4381 gcacggctcg gcgataccca atggccgatc gaattgacgc tggcggcgcg ggacgtgatg
     4441 gggttccgga tgctgctcgg ccgcgaagcg atccggcggc gcttcctcat cgatccggcg
     4501 cggagctttc gccagtccac ccgcccgggc cagccgccga aaaagaagaa gccgaagtga
     4561 agatcgccct gctgtgccgc agcccccagc tttattcgca ccgccggatc atggaggcgg
     4621 cgcgggcgcg cgggcacgat atcgacgcga tcaaccattt gcgctgcacg atcgacatcg
     4681 cctcgcaccg ccccaagatc cattatcagg gccgcgtgct cgccggctat gacgcggtga
     4741 tcccgcggat cggcgcctcg gtcaccttct tcggcaccgc ggtggtgcgg cagttcgaga
     4801 tgatgcgggt ctattcggtc aacgaatcgg tcgcgatcgc ccgctcgcgc gacaagttgc
     4861 ggagcctgca attgctgtcg cgccgcggca tcgggctgcc ggtgacggtg ttcgcgcaca
     4921 agacctccaa cgccgaggac atcgtccgcc tcgccggcgg cgcgccggtc gtgatcaagc
     4981 tgctcgaagg cacccagggg atcggcgtca tcctcggcga aacccccaag gccgccgaaa
     5041 gcatcatcca ggcgttcggg ggggtgaaca ccaacatcct ggtccaggaa tatgtccagg
     5101 aggcgcatgg cgaggatatt cgctgcctgg tgatcggcgg caaggtggtc gcctcgatga
     5161 tccggcgcgg gcgggagggc gaattccggt ccaacctcca ccgcggcgga tcggcgcggg
     5221 cggtgaggat cacgccgcag gagcgggcga ccgcgatcgg cgccgccagg gcgatggggc
     5281 tcaacgtgtg cggcgtcgac atgctgcgat cgaaccacgg gccggtggtg atggaggtga
     5341 attcctcgcc ggggctcgaa ggggtggagc aggcgaccgg cgtcgacgtc gccggcaaga
     5401 tcgtccagtt catcgagaag aatgccgagc cgggcaagac ccggacgcgg ggcaagggct
     5461 aggtggcgcg gcggccgttc agcttcggcg gcgaggaggt gccggccggc gggcggcgcc
     5521 tcgtcagcct gccgatgagc gtgctgtcga accgcacgcc gatggcgctg ccggtcagcg
     5581 tgatccacgg gcggcgcgag gggccgaccc tgttcgtcag cgcggcgctg cacggcgacg
     5641 aggtgaacgg ggtcgagatc tgccggcggc tgctggcgtc gaaggcgctg gcggttgcgc
     5701 gcggcacctt gctggtggtg ccgatcgtca acaatttcgg cttcatcagc catagccgct
     5761 accttcccga ccggcgcgac ctcaatcgca gctttcccgg cagcgcgcgc ggctcgctgg
     5821 cggcgcagct cgcttatgcg ctgatgaccg aggtggtggc gcgcagccag ttcggcatcg
     5881 acctccacag cgccaccatc caccgctcga acctgcccca gatccgcgtc gacagcggct
     5941 atccggcggg gctggaactg gcgcgggcgt tcggcgcccc gatcatcgtc cacagcccga
     6001 cccgcgacgg ctcgctgcgc aagacggcgc aggagcgcgg cgtgccggtc atcgtctacg
     6061 aaggcggcga ggggcttcgc gccgacgaat tctcggtcag cgtggggatg cgcggcgtgc
     6121 tgcgcgtgct tgaccatctc ggcatggtcc gcaagcggac cgcagggagg ctgcgcccgc
     6181 cggcgctggc catgagcagc cgctgggtgc gggcggagga gagcgggctg ttccggccgc
     6241 tcaaggagat tggcgaccgg gtcgcggcgg gcgagcggat cggcatcgtc gccgatccct
     6301 atggcgagat ggaagcggag gtgaccgcgg gctcggccgg catcgtcatc ggccgcgcca
     6361 acaacccggc ggtcaaccag ggcgatgcct tgttccacgt cgcggaggtc gaggacccga
     6421 cccggctcga gcagcattac ggcaccttgt cgcgcgagct ccagtcggag cgcgtgctcg
     6481 acgaggacga gatcctttag gtcgctaagc tcgggtcgag gcggtacatc ttgcgcagca
     6541 gcgccggcca ggcgaggaaa ttggctgcga gcttcaggcc catcgcgccc tgctcggcgg
     6601 cgacttcggg cgtgccctgc ggcgggttgt tgacgtcctt gccggccgac aaggccagga
     6661 tctgcgcctg gcaggcgcgc tccaggaaat agaggcggat gaatgcctcg ccgacattgc
     6721 cgccgaccgc gagcgtgccg tggttcctca gcagcatcgc attcttggag ccgatattgg
     6781 cgaccagccg ctcgcgctcg tcgaggtcga ccgccacccc ttcataatcg tgataggcaa
     6841 gatcgccgcg gatcagcatc gcggtctggg tcagcggcag cagcccctcg gcatgggcgg
     6901 cgaccgcctg gccgtgcggg gtgtggaggt gcatcaccgc ctgcgcgtcg ggccgcgcca
     6961 tgtgcagcgc cgaatggatg gtgaagccgg ccgggttggt gatgaacgga gtcgcgtcga
     7021 ccggattgcc gtccatgtcg accttgacca ggctcgatgc ggtcacttcc tcgaacatca
     7081 aattgtaggg gttcaatagg aaatgatgct ccggcccggg aatgcgggcc gaaaggtggg
     7141 tgaagacgag gtcgtcccag ccataatgcg cgaccagccg gtaggcggcg gcgagatcaa
     7201 cgcggacctt ccattcctcc tcggccacct tgcccttgag cgaggggatc tcgaccgacg
     7261 tcagcggcgc caggtccacc atcccctgcc cgatcgcctc gactctgccc atcgcgcctc
     7321 tcctcctgct accccggcta tagcttattc ggacgccgcg accacgtccg gatgcgcggc
     7381 ggcgaaagcc tcgatccggc cggcctcggc ctcgaccgaa accagccgcg ggaagtcgcc
     7441 caggggaatg tcgaaccggc gcgcattgta gagttgcggg accaggcaga tgtcggcgag
     7501 gctcggcgcg tcgccgaaca ggaagcgccc cgcgcgcggc gcggccatcg cttcgagcgc
     7561 cgccagcccc tcgatcaccc aatgcgcgta ccaggcgctg cgcgtttccg gatcgagccc
     7621 gagcggcccg ctcagatatt tgagcacgcg cagattgttg atcgggtggg tgtcgcaggc
     7681 gaccaccaaa gcaagggcca gcacatgggc gcggtcagcc gggtcccgcg ggagcagcgg
     7741 cggttccggc tcgagcgcgt cgagatagtc gatgatggcg aggctctggg tgagcctgag
     7801 cccgccgatt tcgagcgtcg gcaccagccc ctgcgggttg cgcccggtat agcccggctc
     7861 cttctgcgcg ccttcgagca ggttgatcgg cacgcggtca tgttcgatgc gcttgaggtt
     7921 tagcgcgatc cggacgcggt agccggcgga ggagcggaaa taatcgtaga ggacgggcct
     7981 ggtcatgcgg ctttttagcg aaaccgcggc gcgtttccca ccgccgccgc gaccggcacg
     8041 tcaccgcgct tcaatcgagg acctcgaggc gggggccgcg caggcgggtc aggatcatgt
     8101 cgccgccgga gccgacccgg aactcgccat acagcgcctt ctcgtaagcg gccgcccgtc
     8161 cttcctggaa gaaataggcg ttggtcgcga aattcggctc cccgccgcgg atccggtagc
     8221 gaagcgcgat ctcattggga gcgagcggtc cggcgccggc gaagcgacgg aaatgggcta
     8281 tgttgcgagc gtcaaggccg acgacgatat gaccgtctcc catttcggtg tcgctgaagc
     8341 ccgggaaggc ctgatccgcc acctcgaaat tcagccgcat ataatcgccc tgcatcagtg
     8401 agcgcggatc gatcgggctg agcttcagga gcacgaccct gccgtccgcc agcagccgct
     8461 cgcgctggta gatgccgaaa ttcacggccg cgagaacggc cagcccggcg agcaatgcgg
     8521 ccgccttacg catggccgag ttgctccgca ccggcggcgc cgcggcgcag gaagaggtgg
     8581 gcggcgagca ggcagaggcc agtgacggct aggatcgcgg ctttttcgat cagcgtggcg
     8641 tggaggctgt agtagaaatg ggcgacgaag ccgagcaggc tcaatatgcc cagcgccatc
     8701 aggatgcggt tgccggcggc gaagccaagg agcaggatca gcaaagcgga ggccagcccc
     8761 ggcgcgctca gcgacagcag cccgatcagg agggccgcgc cggcggcgga caaagccgtc
     8821 cggctcgccg gggcaaagcc ttcgcggcgc gacaatgcta cggcgaccca gaccaaagcc
     8881 gctcccgcca acgcctgacc gatcgccggc ccgtgcacca ttatccagct cggggcttcg
     8941 tcgccgcgct cgaaccaggt ttcgacgccg aacaggcgaa acgtctcgac cagcaacagc
     9001 gccaggacga gcccgtagcc gaccgggcgc cacagccctc cgctgccggc ccagcgcgtg
     9061 ggttggagcc acagccacgc gaccgagacg cacaggagcg gtgcggcgaa gccatggagg
     9121 gaaagccggt ggaccgcgag cgcaagcgcg attgcggcgc cggtggtggc gagcacgcgg
     9181 tggaggaaat tgggtaccgc gagggcgagt gcggcctggg tggcggcgac ggccaaatag
     9241 aatccggggc ggtcgggctg gaacaattgc gcgagcccga cgaccagcag catctggccg
     9301 gcgaggctca gcgcaagcgc gaactgctcg acgaagacct gctcgtcgaa gctccggaac
     9361 agagcgaagg cggctccgca gcaggcggcg ccggctattg tggccgaggg gccgtcgtcc
     9421 aggacggagg cgaaagccgc tccgacgaag ccgatcagga acaaggcccc gatccagccg
     9481 gcaaagccga gcatggcgcg gacgtaccaa ggcgattgcg gctgcccgcg cgcgggatag
     9541 ccgccttcga cgagattttc ggcacccagc cggacccaca gcgcctcgcc ggtcatgccg
     9601 gctgctcccg cgcgaccgac ttcagccaga ccgcgccgcc ggccgccatg ccgatcacga
     9661 ccagaccgat caagaggaag ctggcgccat cgtcgcttcc cagcatctgc cgcgacagga
     9721 acaccgccac ggtcacgatc aggctcaaca ggcccgcggc gagcatgaag aggtcgggcc
     9781 gggcgtgccg gtaccaggca taaaggccgc ccagccacgc cagataggcg acggccgcca
     9841 acacgccgcg gccgttctcg gcgtcaagga tcgcccacga aatcaacgcg gtcgccgtgc
     9901 cggcgcttgc cagcgccacc agccggggcg tccagctgtc gcgcagccac gaaaggccga
     9961 ggcggcgccc cgcttcccat gccgcgagcg cgaggccgtt gaccgcgaac agcacccagg
    10021 ccagctcttc cccgtccgag aatagcgaga aatagaaaga agcggccagg ttgaggagga
    10081 cgagccacag cagccataag gggctgaagc gggcgatcag cacccagggc aggatcagca
    10141 ccgcccacca gccgaacagc tcgaacgtgt cggcgccggt ctggtagacc tggcccgcga
    10201 gggcgaggag ggcgccgacc agcaagctca gcagcagcag gatcgccttg ccgcccatgc
    10261 cgtcgaggtc cgtccaacag cagaccagca ggcccgcgca gatcgcggct tcgacgagac
    10321 caaacttggc gaagcggccg agatcgtccc agttgaaggc gaagaagaag atcaccgatg
    10381 cggccagcgc gaccgtgccg agccacaagg agagctgccc gagaaaggtg cgccagtcga
    10441 ccggcgtggg aatcaccccg gccgcgcgca aggcctctcc ctcacggccg ggctcgaccc
    10501 ggcccgcatc aagccattcg aagacttcgc tgcgaagccg gctcatccct gtcccccgcg
    10561 ggccaaaccc gcccggttcg gcgataacag gtcgggcttg cgctggctat cgttacgggg
    10621 ccgtcacgcc gggccggcgc tcccgctact ggccggcttg cgccggcgcg ttcttgcctt
    10681 tgaacccgcg gacatgcgcc accatggcgt cgatctccgg ctccgtcagc gtcccctccc
    10741 agggcggcat atattcgctg ccgccaaccg ccgggccgcc ggccttgatc gcggtgcgga
    10801 ggcgctggtc gttgcgggcg gcctggaagt cggcgcgggt gaagtccggc accccgcctt
    10861 cgatcagttt tgcgtcgggc ccgtcgccct tgccgccggt gccgtggcag cggccgcaat
    10921 tggtgttgaa caggttcgaa gcctgggcgg cggtcacgcc ctgcttctcg gccgccgcct
    10981 tggtcgccgc ctgctcggcg gcggcggtgc gcgcgctcgc gcttcctgcg ggcacgagct
    11041 tgccgatcat gccggtcggc tggtcgttgt cgcccatcat caccaccagc aattcgccgt
    11101 cgggcgcggc ggcgaagccg gtcggcccgc gctgcgacac ttccggggtg cgggcgatga
    11161 tgtcgaagtc ctcgacgctc gcgccggtgg ccggcatggt catgacctgg cccgaataat
    11221 tatccaggaa gacgtaccgg ccctgagcct cgggcaggcg gttgccgcga tagacgaggc
    11281 cgccgatcac cgacctcagc aatgccgtgt ggtgataggt gagcaggggc ggccgctcgc
    11341 ggccatcgat gcgtgcgggg cggggatatt ccttctgcac ctcacgcccc tcgatatagg
    11401 gatattggta attgccgccg cgctcgagaa ggttgacctc ctcccacatg gccgagccga
    11461 cgtcgccggc ccagagcttg ccggtgccgc gatcaaaggc gatgcggaac gggttgcgca
    11521 ggccgtgcgc ccaatattcg ccgagcagct ccggccggtt ggcatagggg ttgtcgagcg
    11581 ggatataata atgggcggtg gtgccgttct cgggctggcg gccgatcggg cggctgatcg
    11641 taccgccctt ttggtcgacg tcgatgcgga ggatgccggc gaccagcgcg cattcgagcc
    11701 tttggtggca cacctccatg tcggattcgc cgaccgcgag atagaggaag ccgtccggcc
    11761 cgaactcgac cgagccgcca ttatgatagc cgttgctgga ccgcccctgc gcgatcagcg
    11821 gcagcccgct cgcggtgcgc gccgcggtgt cgggcagcga gatgtcgtag cggctgaggc
    11881 ggttgatctg ccggtcgcga tgatattcgg tgaagaagac gtagacgaag ccggcattgg
    11941 gtgagccggc gcggccgaac tcggggtgga gatcgaagcc gagcgcgcca ttctccatct
    12001 cgacatagcc gacgccgtcg cggagatcga gcagcagcag cttgttgccg ccgctcggat
    12061 aatcggcgcg gtaaagccgg ccgttgcgtt cgagcacgta gatatggcgc gggtcgccgt
    12121 cggcgaactg ggccatgatc ggagtttcga agcgggtgtc gccgatcgcg ctcaccaccg
    12181 catagggata gtcgccgtcg tcgttggacg aaatgcgcag gttcgtgacc caattgccga
    12241 agtcgcggtc ggtggtgaat ttgtaggcgg cggcgagcgg cgtcatcgcg aacaggatga
    12301 ccagccagat gaagggcgat ttgaggaggc cgaacaggct gccgacgagg ccgccgagct
    12361 ggcccagcgg cgcttggcgg ccgcggatcc agcggatggc gaggaaggcg acgagcagca
    12421 gcgcgaagcc gatcaggaac gcgtcgagca ggaattcggg gctgaaattg atcggcgtca
    12481 ggaagaacag atagagggcc gaggcgaccg cgccggtgag ataggcgtag atttccttgg
    12541 cggcgcggcg cgggctttcg aacgggtccc agccgctgat gaagcgattg gccagaaagg
    12601 cggcggtgca ggcgatcagg aaggcgacga gcaccgtgcg gatgtcggag gcgcggaagt
    12661 aaacgtcggg aatgtcgagg atcgagcgcg gcacgaggtg gacgcgctgc gccatctccg
    12721 cccccagcaa caccgcgacg acgacgatca gcagctcgag cgcaaaatac aggatgcgtg
    12781 acaaaggctt ggttcccccg ggcggcggct ttccgcgcca tcgctcccct ttggtaaaga
    12841 tcacattatc ggaacgccag acggtttggg ggtcttgttc cccggcgacg gccggggtcc
    12901 agtcccggcc cccctctgga ccccggcttt cgccggggaa caatggctta aagcggccgg
    12961 aagaggaggc cgaggcggat gacgcgggcg ggggcgggga agccgatcgt ttcctcataa
    13021 tctcgcgaca ggagattctc gccggggagg gtcagcgcca ccgcctcgct cagccgatat
    13081 tgcgcggccg ccgagacgac cagccgcggc tcgagcgtca cgaggccggt cggcaccgag
    13141 ctgtcgaaat agtcgccggt ataagcggcg ctggcctcga gccgcagccg cgggtccggg
    13201 cgccaatcga gcgcgaccag ccccgaccat tcggggcgcg agcgcagcgg cggcgcatcg
    13261 gccggctggt cggtgtcgag ataggtgagc gccgcctgca ccgccagcgt cgccgccagc
    13321 ggggcgcgga tctccgcctc cacgccctgc gccgtcaccc gcgagcggtt gacgttggtg
    13381 aaggtctcgg cgtcgaagtc gatgaggtcg ctgtagcggc tgtggaaata gccgatgcgg
    13441 gcgaggccgc gcccgtcggc ccaggcctgt tcgaggccga agtcgaaatt gcggctgcgc
    13501 tccggccgca aatcgggatt ggcgatgatc gggtaagcga gcgcgtagag gctcggcagc
    13561 ttatagcctt ccgaccagct gccgaccaag gtgggcccgc ccgccagcgg ctgccagcgc
    13621 gccgaggcgc ggccggtcca ttcggcggcg gcggtggagg ggtcgtcatg ccggatgccg
    13681 aaagtggcgg tcagccgctc ggccgggcgg agggtcgctt cggcgaaggc gctggcgatg
    13741 ctgcgctcga tgcggaagtc ggccgggatg aggatgccga aatcgatggt cccctcgctc
    13801 acgccgcgct cgcccagcca ggcggcgccg aaggcgagct cggccgcctc gccgagccgc
    13861 agccggttgt cgaacacggc ctcggcgcgg cggaagcggc tgtcggagcg gatcgccggc
    13921 accccgtcca gcaccgggcc gggcgcgatg gcgggcgtgt cggcgtcgtc gcgctggcgc
    13981 gacccgctca gcgccagccg cggctgccag gcgccatcgg cggggccggc cagcgtcagg
    14041 ccggcgaggc tcagcgcggt ctcgcgccgc tcgcgctcgc ggttgacggc gagccggggg
    14101 ccgccgctat cctcggggaa gacttcccgg tcggcccgcg cgtgcagcgc cagcgccgac
    14161 aggctgaccg cgcccacttg ctgggtgagg cgcgcgaaca gctggtcgcg ctcgatgtcg
    14221 ctgccctcgg tcaggtcgcc gctgtcggcc gcggagaccc cgccgagcag cgccccgctt
    14281 tcccagccat agccgcccag cgcggtcacc gcgtacccgc cctcgctgtc cgccgccgcc
    14341 cgcgccgaag cgagccgctc gccggcgccg atcgtgcgca gccgcaggtt gacgacgccg
    14401 ctcagcgcgt ccgccccgtg caccgccgac agcccgccct tggccacgtc gatgcggtcg
    14461 agcgcgagcg ggtcgatctg gccgaagtcg aaggcgccgc cgcggctgtt ggtcgggtcg
    14521 gtgaccttga cgccgtcgat cagcaccaaa gtgaaattgg gctcgccgcc gcggacgctg
    14581 agataggagg ggccggccgc gccgcccttg ccgaacgcgc gcacgcccgg cacccgcccg
    14641 agcaggtcga gcacgttggc gggctgcagc agctcgatct cgggggcgga gatcgttgcg
    14701 gtatcgaaac cttcggccgc gtgccccgtc ctagacccgg tgacgacgat cgcctccgcc
    14761 tcctgggccg acacgggcgc cgcgagcacc cccgccgcgg caagggccgc cgccggcagg
    14821 ccgccacgga gggggcgcga aaaccgggtg ctcatcggct gcgggtcagg cgagcgcctc
    14881 gacgatggtg acgttggcga tgccgccgcc ttcgcacatc gtctgcagcc cccagcgctt
    14941 gccgcgcgcg cggagcgcgt ggaccagggt cgccatcagc ttggcgcccg acgccccgag
    15001 cgggtggccg agcgcaatcg cgccgccatt gacgttgagc cggtcgggat cggcgccggt
    15061 ctccttaagc cacgccagcg gcaccgaggc gaaggcctcg ttcacttcgt aaaggtcgat
    15121 gtcggcgatg ccgagcccgg cgcgacccag cgcgcgccgc gtcgccggga tcggctcttc
    15181 gagcatgatc accgggtcgc cggccgttac ggtcaaattg tggatgcggg cgagcggggt
    15241 gaggccatgg tccttcagcg cctgctcgct caccaccatc acgccgctgg cgccatcgca
    15301 aatttgcgaa gcgttggccg cgctgatccg gccgccctcg cgcagcagct tgaccgacgc
    15361 gatgccttcg atcgtggcgt catagcgtat gccctcgtcg gcgcggtgct cgcccgcctc
    15421 ggtggcgagc ggcacgatct cgcctgcgaa cgcaccggcc tgggtcgcgc gcgccgcctt
    15481 gatgtggctg tcgagcgcga aggcgtcgag cgcgtcgcgg ctgaagccgt atttctcggc
    15541 gatcatctcg gcgccgtgaa actggctgaa catcgccacc ccgaaccgct cctggatgcg
    15601 ctgcgagaac ggcccggtgc cgatgccgtg ctgcatgggc agggtgaagg gcaggcccat
    15661 cggcacccgg ctcatcgatt cgacgccggc cgcgatcacc aaatcctggg tgccgctcat
    15721 caccgcctgg gcggcgaagt ggatcgattg ctgcgaggag ccgcattgcc ggtcgatggt
    15781 gacggccggc acgctctgcg gcagcttcga ggccagcacc gcgttgcggc cgacatggaa
    15841 ggcctgctcg ccggcctgcc cgacgcagcc catcaccacg tcgtcgatag cggcgggatc
    15901 gacgccgctg cggtcgacca gcgcgttcag cacttccgcg gccatgtcgg cggggtgcca
    15961 gccggccagc gcgccgccgc gcttgccgcc ggcggtgcgg acggcttcga cgatataggc
    16021 ttcggccatc tttcccccgc gccctccaag ccgcctatgg aagcgtcagc caggatcgcg
    16081 gcggaaagct gacggcgccg gtgccgctta ggtgactcga acacctgacc ccatcattac
    16141 gaatgatgtg ctctaccagc tgagctaaag cggctatccg gcgagggcgc gcattatcag
    16201 cggccccggc cgaacacaag cgcggccggc gcgccctgcc acccgcgccg acacccgctt
    16261 cgcccgcaga cttaacgctt tttcaaccat ggccggtgca gggtccggcg acgatgattg
    16321 agctgcgagg acagcgacga tggatagttt ccgggggttc gatgacagtg ccgaggcaga
    16381 cgtcctcggg gaggacgagc cctcgatcga gcggccgccg gaaatcggcg tcgacgagcg
    16441 ccgcatgcat gtccgtgcct ataattattg ggtgtcgctg ctcgacggcc gcgcctaccc
    16501 ctcgatccgc gacctggatc cgggcaatct cgacgatttc gggccgtaca gcgtgctgct
    16561 caacttctcg tcggacgccg acaatccgga agtcgccttc atcggccgcg cgctccgcca
    16621 ggagaccggg ctcggcgacg acatcaagac catctccgac gtgccgagcc gatcgctgct
    16681 gtcgcggctg accgaccatt atatgcagat catcgccaat tgcgcgccga tcggcttcga
    16741 agcggagttc gtcggccaga gcggccacca gaccatgtat cgcggcatct tgatgccgct
    16801 ttcgtcggac ggcgacacga tcgatttcat ctacggcgtc atcaactgga aggaagtggc
    16861 cgacggcaag accgccgccg ccctcgcccg cgaagtcagc cgcgccgccg ccgcgccggc
    16921 gatcgaggcg agcccagtct gggccgacgg ccccaatgcc gagccgctgg acgaggcctt
    16981 ttccgccttg ccgcgcgagg cgagctacga gcttgccgtc gacgacgacc agccctcgct
    17041 cgatctgccg ctggccgccg acgccgccct tgccgaccgg ctgtgcgccg cgcgggactg
    17101 cgccgaagcg gtgaagtccg ccgatgcgcg cagccgctcc gcgctctacc gcgcgctcgg
    17161 ccacgcctat gatttcgcgc tcgcggccga ggcttcgccg gaagattatg ccgaattgct
    17221 cgaagatgcg ggcctcaagg cccaggcgcg cgcgccgatg acgccgatcg ccaagctcgt
    17281 cttcggcgcc ggctacgaca agacccggct caccgaattc gccgccgcgc tctcctgcgc
    17341 gcgccgcgaa ggcgtggccc aaagcgggtt cgtcgcctgg ctcgaaggct ttggcggggg
    17401 cctcaagggc gccgtcgggg ccgaacgcct ggcgcgccgc ccggcggcca agcccgaccg
    17461 ctttgaggcg gtgcgcgcag cgctccggca agtggtcccg gtcgcgcacg tctccgtccc
    17521 gagcgaggtc caggagggcg atttcgtcct gctcgtcgcc cgccgcgagg ccgatgg
//
LOCUS       DASVRK010000294         8851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_161514, whole genome shotgun sequence.
ACCESSION   DASVRK010000294 DASVRK010000000
VERSION     DASVRK010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161514"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..356)
                     /locus_tag="VF589_12345"
     CDS             complement(21..356)
                     /locus_tag="VF589_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8258412.1"
                     /translation="MAPPRYQVRLTQGAERDLEALYDYLAEHRSADEAEALLEALVGK
                     VETLEDFPERGSVPKELEALGMREFRQILMGRYRMIYRVAGAAVFVMVIADGRRDMQS
                     LLERRLLGR"
     gene            complement(356..631)
                     /locus_tag="VF589_12350"
     CDS             complement(356..631)
                     /locus_tag="VF589_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX8258413.1"
                     /translation="MRYSSQVRPISFLKAHSAEVLADLAERREPMVITQNGEAKAVIQ
                     DVASYEEGQEMLALLKLLALGGRDIDEGRTTPARDVVARLRAKTAAR"
     gene            complement(712..3255)
                     /locus_tag="VF589_12355"
     CDS             complement(712..3255)
                     /locus_tag="VF589_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HEX8258414.1"
                     /translation="MRIEIPEFALVLLVGASGTGKSSFAATHFLPTEVVSSDRARAWV
                     ADDETDQAVTGDAFDLVHAVVEKRLKHRRFTVVDATNVQPEARKGLLALARKWHALAV
                     GIVFDLPESVAVARNAERPDRQFGAGPVRRQMQTLKRSMRGMQKEGLRYLHVLKSTEE
                     TEAVSIVRTKLWTDRREDKGPFDIIGDIHGCGDELEALLATLGYAVRWDGKEVEVTPP
                     EGRRAIFIGDLVDRGPRSPDVLRIARHMVERGTALAVVGNHDDKLKRHLSGKNVKATH
                     GLAETIEQLAAEPAGFARDIRDWLDKLISHYVLDEGRLVVAHAGMKEEMQGRASGAVR
                     SFAMYGETSGEVDEFGLPVRYDWAADYKGRARVVYGHTPVLEAGWVNGTLCIDTGCCF
                     GGKLTALRYPEMELVEVPAARTYYEPIRPLAAPPAATGDAHPHQLDIADVLGKQAIET
                     RDLGMVIVREEYAAAALEVMSRHAVDPRWLIHLPPTMSPPETSAREDWLERPEEALAY
                     YGGKGISALMLQEKHMGSRGLVVLARTVEAAARRFGIADGARGVVLSRTGRRFFGDAE
                     LEAAALHRIDAAMAEAGLWEELGSDWVLWDAEILPWSMKAGPLIHEQYAPVGAAAAAG
                     LGALGEALAKARARGLAVDELAATAAVRLDDARRYRDAYNRYVAPFAGIGDLRIAPFH
                     LLASERAVHSDKDHRWHMMQAHRLAAADPELVLATRYRELDLGDAAAVEAAIGWWEEM
                     TGAGGEGMVVKPLVYTPRGKRGLLQPAIKCRGREYLRIIYGPDYDRPEHLVRLKQRGL
                     GHKRSMAIREFALGLEALHRFVEQAPLTRVHQCVFGVLAMESEPVDPRL"
     gene            complement(3245..4645)
                     /locus_tag="VF589_12360"
     CDS             complement(3245..4645)
                     /locus_tag="VF589_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HEX8258415.1"
                     /translation="MLLTLTTTHRPATDLGYVLMKHPDNLHVVELPFGRATIFYPEAG
                     EERCTAALTVEVDPVELVRGKGGGAGLQDQYVNDRPYAASSLLSVALGRALGTAMSGR
                     SKLRQELADTALPLEATVTPLPARGGGAGLIARLFEPLGYAVEARAIAMDPAHPEWGD
                     SPYVELRLTGRARVADLLNHLFVLIPVLDKAKHYYIGEDEVAKLLRKGEGWLDAHPER
                     ELIVRRYLKGFKSLVRIAQRGLDEAPEVEEAEASFARDAEEEALEKPQRLNDVRLERV
                     AAVLKELGAASVLDLGCGEGKLLRHLLQKRGFTRIVGMEVAPRVLAVAAERLRLESMP
                     DLQRKRIELIQGSLVYRDDRLKGFDAAAVVEVIEHMDADRLPSFEQALFGHARPGAVV
                     ITTPNADYNALFPSLPAGRMRHPDHRFEWSRSEFEHWARSVAAAHRYEVRFEAIGPGD
                     AALGGPTQMGVFTRAD"
     gene            complement(4963..5955)
                     /locus_tag="VF589_12365"
     CDS             complement(4963..5955)
                     /locus_tag="VF589_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8258416.1"
                     /translation="MERRHLGKKGPAVSALGLGCMGMSDLYGPADRAESLATIAMAAE
                     RGITLIDTGDFYGMGDNEMLIGEALKALPRDRLTLSVKFGALRDADGGWRGYDSRPAA
                     VANFAAYSLRRLGTDHIDIYRPARLDPDVPIEETVGAIAELIRKGHVRHIGLSEMGPE
                     TIRRAHAVHPIADLQIEYSLLSRGIEAEILATCRELGIGITAYGVLSRGLISGHWSKG
                     CGGAGDFRAMSPRFQGGNLDRNLELVEALRRIAEAKGVSVAQIAIAWVAARGADIVPL
                     VGARRRDRLDEALGALDVELRAEDMDAIERAVPPGAAAGARYPEPMLAHMDSER"
     gene            complement(5979..6716)
                     /locus_tag="VF589_12370"
     CDS             complement(5979..6716)
                     /locus_tag="VF589_12370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8258417.1"
                     /translation="MADIGNKTILITGASSGIGEATARELGAAGAKLLLGARRTDRLE
                     RLAGEIEAAGGTVGWRPLDVTRRDDFEAFAADAEARFGRIDAIVNNAGVMPLSPLAAL
                     KTDEWDRMIDVNVRGVLNGIVAVLPRFVAQGSGHVVNVASMGAHYVVPTGAVYCATKF
                     AVWAITDGLRREHENIRATIISPGVVASELGHDITDPATAAAIAVFRERQLQPDSIAR
                     AIRFAIEQPDDVDVGEIIVRPTASNVI"
     gene            6860..7774
                     /locus_tag="VF589_12375"
     CDS             6860..7774
                     /locus_tag="VF589_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8258418.1"
                     /translation="MDIIEEMGGIIARHAPGDGMHDSAIERLVLIRSSTPTEPLHVVY
                     DPALCLVVQGCKQAVLADRTYRYDAANFLLVTVDLPIVGCVVEASPERPYLCIALRLN
                     LPVLAELMLDCAPPASAAAPAPTAATPGIALGETDAGLLDAVCRLLRLLDSPEDAAML
                     GPLAEREILYRLLRGPQGAIARHIAAADSHLRRISRAIGWIRRHFAEPISVERLAAEA
                     AMSPSSFHEHFRAVTAMSPLQFRTRLRLMEARRLMVSEGLDAAGAGFRVGYESPSQFS
                     RDYSRTFGLPPLRDAARLRASPQPALVA"
     gene            7969..>8851
                     /locus_tag="VF589_12380"
     CDS             7969..>8851
                     /locus_tag="VF589_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258419.1"
                     /translation="MGMAMSEQICWINIGPEGRFRRSGQISTSPADVDRLLGAGLAGR
                     QRLVLHFHGGLVDESSGRKIAEAMAAVYGDSAASIGLVWETGILEVFRDNLNKLHDRR
                     AFRKALSWIIAKAAPALGAAEGARGATGGSLDPKAIERMLESEEGVALLDAALSADDV
                     AGGAETMARGGAGEEGLLVETIAFELELGLGSDPELVTLVETGAEGAGPIRKKLELDD
                     SQRGASLAALALLLAKVVIAVIGRYRKGTHHDLLPTCVEELLRAAFLGEIGVSAWSDM
                     KRKALEMWTDDGADPGEE"
BASE COUNT         1320 a         3293 c         2874 g         1364 t
ORIGIN      
        1 gcgcgggggc cgccggtccc tcagcgcccc agcaatctcc gttccagcag cgactgcata
       61 tcccggcgcc cgtcggcgat caccatcacg aacaccgccg cgccggccac gcggtagatc
      121 atcctgtacc ggcccatcag gatctggcgg aattcccgca tgcccagtgc ctccagttcc
      181 ttgggcacgc tgccgcgctc ggggaaatcc tcgagcgtct cgaccttgcc gacgagcgcc
      241 tcgagcagcg cctcggcctc atccgccgag cggtgctcgg ccagataatc gtacagcgcc
      301 tccaggtccc gctccgcacc ctgcgtcagc cgaacctggt agcggggcgg cgccatcagc
      361 gggccgcggt cttcgccctc agccgggcca ccacgtcccg cgccggcgtc gtgcggccct
      421 catcgatgtc gcgcccgccg agcgccagca gcttcagcag cgccagcatc tcctgcccct
      481 cctcatagga ggcgacatcc tggatcaccg ccttggcctc gccattctgg gtgatcacca
      541 tcggctcgcg ccgctcggca aggtcggcca gcacctcggc cgaatgcgcc ttcaaaaagc
      601 tgatcggccg cacctgcgaa gaataacgca ttcgaattac ctcaccaaat tcggtccgaa
      661 tatagacctc cctcggaccg aggcaagtgc acatgccggc ggtgacccgc tctaaagccg
      721 cggatcgacc ggctcgcttt ccatcgcgag cactccgaac acgcattgat ggacccgcgt
      781 cagcggcgcc tgttcgacga agcggtgcag cgcctcgagg ccgagcgcga actcgcgtat
      841 cgccattgac cgcttgtggc cgagcccgcg ctgcttcagc cgcaccagat gctccggccg
      901 gtcgtaatcg gggccgtaga tgatccgcag atattcgcgg ccgcggcact tgatggccgg
      961 ctggagcagg ccccttttgc cgcgcggcgt gtagaccagc ggcttcacca ccatgccctc
     1021 gccgccggcc ccggtcatct cctcccacca gccgatcgcc gcctcgaccg cggcggcatc
     1081 gccgaggtca agctcgcgat agcgggtcgc cagcaccagc tccggatcgg ccgcggccag
     1141 ccggtgcgcc tgcatcatgt gccagcgatg gtccttgtcc gaatgcaccg cccgctccga
     1201 ggcgagcaga tggaacggcg cgatgcgaag gtcgccgatc cccgcgaacg gcgcgacata
     1261 gcgattatag gcgtcgcggt agcgccgggc gtcgtccagc cgcaccgccg ccgtcgccgc
     1321 gagttcgtcc accgccagcc cgcgcgcccg cgccttggcg agcgcctcgc ccagcgcgcc
     1381 gagccccgcc gccgcggccg cgccgaccgg cgcatattgc tcgtggatca gcggcccggc
     1441 cttcatgctc cacggcagga tctcggcatc ccacagcacc cagtcgctgc cgagctcctc
     1501 ccacaggccc gcctccgcca tcgccgcatc gatccggtgc agggccgcgg cttccagttc
     1561 cgcgtcgccg aagaagcgcc gcccggtccg gctcagcacc acgccgcggg cgccgtcggc
     1621 gattccgaac cgccgcgccg ccgcctcgac cgtgcgcgcg agcacgacca gcccgcgcga
     1681 tcccatatgc ttctcctgaa gcatgagcgc ggaaatgccc ttgccgccat aataagccag
     1741 cgcttcctcc ggccgctcga gccagtcctc gcgcgccgaa gtctcgggcg gcgacatggt
     1801 cggcggcagg tggatcagcc agcgcggatc gaccgcatgg cggctcatca cttcgagcgc
     1861 ggcggcggca tattcctcgc gcacgatcac catgccgagg tcgcgagtct cgatcgcctg
     1921 cttgccgagc acgtcggcga tatcgagctg gtgcggatgc gcgtcgccgg tcgcggccgg
     1981 cggagcggcg agcggccgga tcggctcata ataagtccgc gccgccggca cctcgaccag
     2041 ctccatctcc ggatagcgca aggcggtgag cttgccgccg aagcagcagc cggtgtcgat
     2101 gcagagcgtg ccgttgaccc agcccgcctc cagcaccggc gtgtggccgt aaaccacccg
     2161 cgcccgcccc ttataatcgg ccgcccaatc gtagcggacc ggcaggccga actcgtccac
     2221 ctcgccgctg gtctcgccat acattgcgaa gctgcgcacc gcgcccgacg cgcggccctg
     2281 catctcctcc ttcatcccgg catgggcgac gaccagccgg ccttcgtcga gcacataatg
     2341 gctgatcagc ttgtcgagcc agtcgcggat atcgcgcgcg aagccggccg gctcggcggc
     2401 aagctgctcg atcgtctcgg cgaggccgtg cgtcgctttg acgttcttgc ccgacagatg
     2461 ccgcttcaac ttgtcgtcat ggttccccac caccgccagc gccgtgccgc gctcgaccat
     2521 gtgccgggcg atcctcagca cgtcgggcga gcgcgggccg cggtcgacca ggtcgccgat
     2581 gaagatcgcc cggcgcccct cgggcggcgt cacctcgacc tccttgccgt cccagcgcac
     2641 cgcatagccg agcgtggcga gcagcgcctc cagctcgtcg ccgcagccat ggatgtcgcc
     2701 aatgatgtcg aacggcccct tatcctcgcg ccggtcggtc cacagcttgg tccggacgat
     2761 cgataccgcc tccgtctcct cggtcgactt cagcacgtgg aggtagcgca gcccctcctt
     2821 ctgcatgccg cgcatcgacc gcttgagcgt ctgcatctgc cgccgcaccg gccccgcccc
     2881 gaattgccgg tccggccgct cggcgttgcg cgcgacggcg acgctttccg gcaggtcgaa
     2941 cacgatcccg accgccagcg cgtgccactt gcgcgcgagc gcgagcagcc ccttgcgcgc
     3001 ctccggctgc acgttggtgg catcgaccac ggtgaagcgg cggtgcttca gccgtttctc
     3061 gaccaccgca tggaccaggt cgaaggcgtc gccggtcacc gcctggtcgg tctcgtcgtc
     3121 cgccacccag gcgcgcgcgc ggtccgacga gaccacttcg gtcggcagga agtgcgtggc
     3181 ggcgaagctc gacttgccgg tgccggacgc ccccaccagc agcaccaggg cgaattccgg
     3241 tatctcaatc cgcacgggtg aacactccca tctgggtcgg cccgcccagc gccgcgtcgc
     3301 ccgggccgat cgcttcgaag cgcacctcgt aacgatgcgc cgctgcgacg ctgcgcgccc
     3361 aatgctcgaa ttccgatcgg ctccattcga agcgatggtc ggggtggcgc atcctgcccg
     3421 cgggcaggct cgggaagagc gcgttataat cggcattggg cgtcgtgatc accaccgcgc
     3481 ccggccgcgc atggccgaac agcgcctgct cgaacgaggg cagccggtcg gcgtccatat
     3541 gctcgatcac ctcgaccacc gccgccgcgt cgaacccttt gagccggtcg tcgcgataga
     3601 ccagcgaacc ctgaatgagc tcgatccgct tgcgctggag gtcgggcatg ctctccagcc
     3661 gcagccgctc cgccgcgacg gcgaggaccc gcggcgccac ctccatgccg acgatgcggg
     3721 tgaaaccgcg cttctggagc aggtggcgca gcagcttgcc ctcgccgcag ccgaggtcga
     3781 gcacgctggc ggcgccaagc tccttcagca ccgccgccac ccgctccagc cggacgtcgt
     3841 tgagccgctg cggcttttcg agcgcttcct cctcggcgtc gcgggcgaag ctcgcctccg
     3901 cctcctccac ttccggcgcc tcgtcgagcc cgcgctgcgc gatccgcacc agcgacttga
     3961 accctttcag atagcgccgg acgatcagct cgcgctccgg atgcgcgtcc agccagcctt
     4021 cgcccttgcg gagcagcttc gcgacctcgt cctcgccgat ataataatgt ttggccttgt
     4081 cgagcaccgg gatcagcacg aacagatggt tgagcaggtc cgccacgcgc gcccggccgg
     4141 tcagccgcag ctcgacatag gggctgtcgc cccattcggg atgggccggg tccatcgcga
     4201 tcgcccgcgc ttcgacggca taaccgagcg gctcgaacag ccgcgcgatc agcccggcgc
     4261 cgccgccccg cgccggcagc ggcgtcaccg tcgcctccag cggcagcgcc gtgtcggcca
     4321 gctcctggcg aagcttggac cgcccgctca tcgccgtgcc cagcgcccgc cccagcgcca
     4381 ccgacagcaa tgacgaggcg gcataaggcc ggtcgttgac atattggtcc tgcagcccgg
     4441 cgccgccgcc tttgccgcgc accagctcga ccgggtccac ctcgacagtg agcgcggccg
     4501 tgcagcgttc ctcccccgcc tccgggtaga agatggtcgc ccgcccgaac ggcagctcga
     4561 cgacatgcag attgtccgga tgcttcatca gcacatagcc gaggtcggtg gccgggcggt
     4621 gggtggtggt gagcgtcagc agcatcgatg ttccagcatg aaaaagccct tccgaagggt
     4681 tcgaaagggc cgcctgcaaa tccgggtagg ggtggatcta aagcggggcc cgggggcccg
     4741 tattatggcc gatcgccccg ccgaacgcgg ccgccgccgc gccgcggaag cgacgggcgg
     4801 aaaaccggat cggggcctcg atctgcatgc caagtgcttg gaaccgccac ccgccgccgt
     4861 caagccggcc cggccgtctt tggaggcgca ccggcggaag gcggcgaaga tcgagcccga
     4921 attccgtttc aggctaggcg gcctccccgc ccacgggcgt cctcaccgct cgctgtccat
     4981 gtgcgcgagc atcggctcgg ggtagcgcgc gcccgccgcc gcgccgggcg gcaccgcgcg
     5041 ctcgatcgcg tccatgtcct cggcccgaag ctcgacgtcg agcgcgccca gcgcctcgtc
     5101 gagccggtcg cgccgccgcg cgcccaccag cggcacgatg tccgccccgc gcgccgccac
     5161 ccaggcgatg gcgatctggg cgacgctgac gccctttgcc tcggcgatcc gccgcagcgc
     5221 ctcgaccagc tcgaggttgc ggtcgagatt gccgccctgg aagcgcgggc tcatcgcccg
     5281 gaaatccccg gcgccgccgc agcccttgga ccaatggccg ctgatcaggc cgcgcgacag
     5341 cacgccataa gcggtgatgc cgatgccgag ctcgcggcag gtcgccaata tctccgcctc
     5401 gatgccgcgc gagagcaggg aatattcgat ctggaggtcg gcgatcggat ggaccgcatg
     5461 cgcgcgccgg atcgtctccg gtcccatttc ggacaggccg atatggcgga catggccctt
     5521 gcggatgagc tcggcgatgg cgccgaccgt ctcctcgatc ggcacatcgg ggtcgagccg
     5581 cgccggccgg tagatgtcga tatggtcggt gccaagccgc cgcagcgaat aagccgcgaa
     5641 attggccacc gccgccggcc gcgaatcata gccgcgccag ccgccgtccg cgtcacggag
     5701 cgcgccgaat ttgacgctga gcgtcagccg gtcgcgcggc aacgccttca gcgcctcgcc
     5761 gatcagcatc tcgttatcgc ccatgccgta aaagtcgccg gtgtcgatga gcgtgatgcc
     5821 gcgctcggcc gccatcgcga tcgtcgcgag gctctcggcc cggtcggccg gcccataaag
     5881 gtccgacatg cccatgcagc cgagcccgag cgccgagacc gccggcccct tctttcccaa
     5941 atgtcttcgt tccatggctt cgctcctcct gggttcgctt agatgacgtt gctggcggtc
     6001 ggtcggacga tgatctcgcc gacgtcgaca tcgtccggct gctcgatcgc gaagcggatc
     6061 gcgcgggcga tcgagtcggg ctggagctgg cgctcgcgga acaccgcgat ggcggcggcg
     6121 gtggcgggat cggtgatgtc gtggccgagc tccgaggcga ccacgcccgg cgagatgatg
     6181 gtggcgcgga tattttcatg ctcgcgccgg aggccgtcgg tgatcgccca caccgcgaac
     6241 ttggtcgcgc aatagacggc gccggtcggc accacataat gggcgcccat cgaggcgacg
     6301 ttgacgacat ggccggaccc ttgcgccacg aagcgcggca gcaccgccac aatgccgttg
     6361 aggacgcccc tcacattgac gtcgatcatc cggtcccatt cgtcggtctt gagcgcggcg
     6421 agcggcgaca gcggcatcac gccggcattg ttgacgatcg cgtcgatccg cccgaagcgc
     6481 gcctcggcat cggccgcgaa cgcctcgaaa tcgtcgcgcc gggtcacgtc gagcgggcgc
     6541 caccccaccg tgccgccggc ggcctctatc tcgccggcca gcctttcgag ccggtcggtg
     6601 cggcgcgcgc cgagcagcag tttcgcgccg gccgcgccga gctcgcgcgc cgtcgcctcg
     6661 ccgatcccgc tcgacgcgcc ggtgatgagg atggtcttgt tgccgatatc ggccatgggg
     6721 aagctcctgt tgttggcagg cttcaaatgg ccccggcggc accgacagcg gtagaacggc
     6781 agtccgcgat ccttgcacga tcctccgaac cgcttccgcg tcgcttgcgc gggcgcggga
     6841 agcatgggaa gccgcattca tggacatcat cgaggaaatg ggcggcatca tcgcccgcca
     6901 cgcccccggc gacggcatgc acgacagcgc gatcgagcgg ctggtgctta tccgatcctc
     6961 caccccgacc gagccgctcc acgtcgtcta cgatccggcc ttgtgcctgg tcgtccaggg
     7021 ctgcaagcag gccgtgctcg ccgaccggac ctaccgctac gacgccgcca atttcctgct
     7081 cgttacggtc gacctgccga tcgtcggctg cgtcgtcgag gcgagccccg agcggcccta
     7141 tctgtgcatc gcgctccggc tgaacctgcc ggtgcttgcc gagctgatgc tcgactgcgc
     7201 cccgcccgcc tccgccgctg cacccgcacc caccgccgcc acgcccggca tcgcgctcgg
     7261 cgagaccgac gccggcctgc tcgacgccgt ctgccggctc ctgcgcctgc tcgactcgcc
     7321 cgaagacgcc gccatgctcg gcccgctcgc ggagcgcgag atactctacc ggctgctgcg
     7381 cgggccgcag ggcgcgatcg cccgccatat cgccgccgcc gacagccatt tgcgccgcat
     7441 cagccgcgcg atcggctgga tccgccgcca tttcgccgag ccgatcagcg tcgagcggct
     7501 cgccgccgaa gcggcgatga gcccgtcatc cttccacgag catttccgcg ccgtcaccgc
     7561 gatgagcccg ctccagttcc gcacccgcct gcgcctgatg gaggcgcgcc ggctgatggt
     7621 ctccgaaggc ctcgacgccg ccggcgccgg cttccgcgtc ggctatgaaa gcccgtcgca
     7681 gttcagccgc gactatagcc gcaccttcgg cctccccccg ctccgcgacg ccgcccgcct
     7741 ccgcgccagc ccccagccgg cgctggtggc gtaacctcgc cgccgccccg gactgggcca
     7801 gaatcctggc ccttggcgga aaagtcgcgg tcgaccggct cgacaatcgc gtttcgtcgg
     7861 taatgggtgg ctaatcggac cgactagcgc ttgtgcgtgt caccgcaggc tctatcctca
     7921 ccggctcggt cccgatgcgt tgatcagtgc cgccgggtcc gtcagcgaat ggggatggcc
     7981 atgagcgaac agatttgctg gatcaacatc ggcccggaag gtcgcttccg caggagcggc
     8041 cagatctcca cgtctcctgc cgatgtggat cggctgctcg gagccgggct ggcgggccgc
     8101 cagcggcttg tccttcactt ccacggcggg ctcgtggacg agagctccgg caggaagatc
     8161 gccgaggcga tggcggcggt ttacggagat tccgcggcgt ctatcggtct cgtatgggag
     8221 acgggcatcc tggaggtgtt cagggacaat ctcaacaagc tgcacgatcg ccgcgccttc
     8281 aggaaggcgc tgtcatggat catcgccaag gccgcgcccg cgcttggcgc cgctgaaggg
     8341 gcgcgcggag ccaccggggg aagcctcgat ccgaaggcga tcgagcgcat gctcgagagc
     8401 gaggaaggtg tcgccctcct cgacgccgcc ctgagcgccg atgacgtggc cggcggcgcc
     8461 gagaccatgg cgaggggggg cgcgggggaa gaagggttac tggtcgaaac catcgccttc
     8521 gagctggagc tcggtctcgg ctccgatccc gagttggtga cgctggtcga gaccggtgcc
     8581 gagggcgccg ggccgatccg caagaagctc gagctcgacg actcccagcg aggcgcgtcg
     8641 ctcgcggcct tggcgctgct gctggctaaa gtcgtgatcg cggtgatcgg ccggtatcgc
     8701 aagggcactc accatgatct gctgcctacc tgcgtggagg agttgctgcg cgccgccttc
     8761 ctcggggaga tcggggtttc ggcctggtcg gatatgaaac gcaaggcgct cgaaatgtgg
     8821 accgacgatg gcgccgatcc aggggaggag g
//
LOCUS       DASVRK010000295         6539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_552784, whole genome shotgun sequence.
ACCESSION   DASVRK010000295 DASVRK010000000
VERSION     DASVRK010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552784"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VF589_12385"
     CDS             <1..572
                     /locus_tag="VF589_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HEX8258420.1"
                     /translation="TGGAGYDELYGGLGSDRMDGGSTGDYLEGFDGNDTLFGGGANDF
                     ITGDNGMDRLIGGSGDDRLFAGTGNDLLIGGTGVDLLAGELGADSYRFSDGHTGSTAA
                     LADTIEGFSRGEGDVIDLSRVDAATGAGNDAFSFIGDGAFSGTAGELRYERNGLGVMV
                     EADRDGDSMADFYIQVSGPIELVASDFVL"
     gene            681..863
                     /locus_tag="VF589_12390"
     CDS             681..863
                     /locus_tag="VF589_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258421.1"
                     /translation="MALPAIAGGALDGTQEPLLLALLPGAAAAIAAILLAAWTRSAFA
                     PRLVLLIAWYVYLSAA"
     gene            complement(873..1709)
                     /locus_tag="VF589_12395"
     CDS             complement(873..1709)
                     /locus_tag="VF589_12395"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8258422.1"
                     /translation="MPNTILITGASSGFGEATARLFAERGWNVAATMRRPEAGAALAR
                     LDNVLVTRLDVEDQPSIEAAVAETIERFGRIDAVVNNAGFGLMGIFEATPWAKIREQF
                     EVNLFGAMAVIRAVLPHLRANRSGVIANVSSGAGGFALPLISLYTSSKFALEGFSESL
                     AYELADLGVRVKIVEPGGVTSTGFTARSGEEASLVGGLSDYRPFLDGAGAIFQGLVKA
                     RAGATSEEVAEVIHRAVTDGTDQLRYIATEDIKPMLAMRRETSEAEYIAYMRERFAPK
                     IG"
     gene            complement(1756..2487)
                     /locus_tag="VF589_12400"
     CDS             complement(1756..2487)
                     /locus_tag="VF589_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8258423.1"
                     /translation="MPPDAHRRPDPGRIRNIVLVHGAFVDGSGWEGVYRLLSAKGYEV
                     TVVQHPTASLAEDVAYVARAIAAQDGPVALVGHSYGGAIITEAGRDPKVKALVYIAGW
                     VPDSGQSVLDLIGQLPAGAPSAPVLPPSDGFLFVDPVRFPAAFAADVDPAKAQFMAIA
                     QAPWGVAAASATVGEPAWKTKPSWYLVASEDRMIPPEAQRTMAARAGANVTEAAGSHA
                     IFVAKPESTAALIEAAAEGALAAVA"
     gene            2789..5113
                     /locus_tag="VF589_12405"
     CDS             2789..5113
                     /locus_tag="VF589_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258424.1"
                     /translation="MADLIVGDGGFGTIQAAVDAASDGDTIIVAAGTYVEQVIVQNRN
                     GLTIRAADGEQVTLRSPADLVETARSSSDREIHSVFTVKDSSEIVLEGIDVDGEGRGT
                     TVDEGGGAGIANFYGIFYRNASGGLTDVDIVGVRDPYPGGTTPGGQPEVDAVQRGIAV
                     VADNDSLRAFAITGGTISDFQKQAGLFSRADLAITGVTVTGGGAQPVMAQNGFSVTNS
                     SGIVSGNTFSGIGYAGPAPAYSGAILASSNTDLAITGNTITGANGDSTAAKVVGIWVY
                     VASAANNGVEISGNTISGVDVGINASGNFGANGILIRDNEVTDLDLADPYAYGVNFDP
                     LATLPTAFEVDGSARSDILRGAAGGDTLSGLGGKDEIIGCGGDDLIDGGAGDDVLDGD
                     DGDDRLEGGAGDDDLDGGAGSDTVSFSTAMAGVEVDLAVAGPQNTGDGSDLIVNIENA
                     LGSEFADYISGNAGSNHLRGGAGADRLFGRSEADRLSGDGGDDILDGGIGLDEMAGGA
                     GDDLYVARETGDVATEAAEQGHDTVKATADYTLGDHVEDLYLQGLAVRGTGNGLDNRV
                     VGTNGGNVLEGLAGNDILYGYSGMDEMDGGEGADTFYGGLNNDALDGGSGNDRLNGEA
                     GADFIEGGDDDDHLFGRDGADELLGGQGMDRLEGGAQTDILTGGAGLDVFGFGNGDVP
                     GTRFSADRITDFSQAEGDRIHLRQLDANLGIAGDQNFHFVGESHFSGNAGEVRFDHSG
                     GNTYVQGDVDGDRAADFYVRVDGIHSLVVTDFVL"
     gene            complement(5189..5262)
                     /locus_tag="VF589_12410"
     tRNA            complement(5189..5262)
                     /locus_tag="VF589_12410"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5228..5230),aa:Cys,seq:gca)
     gene            complement(5559..5996)
                     /locus_tag="VF589_12415"
     CDS             complement(5559..5996)
                     /locus_tag="VF589_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258425.1"
                     /translation="MRIRVVALLAAMLVTGCGDGSRKVAEALGNSAWAGSAIKRAAAP
                     ATAAPAPLAAADVALPVYGADHDLRAALEPLMPLCLAGLAAEMAGGEFTAAVDELTAG
                     WPADKLARAREVCTAFRFGAIGIMLITEKTREAEAAAAGAKRI"
     gene            complement(6111..>6539)
                     /locus_tag="VF589_12420"
     CDS             complement(6111..>6539)
                     /locus_tag="VF589_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HEX8258426.1"
                     /translation="AGIDATPLRFAHFLAQLGHESGGFRYMEEIASGEAYEGRRDLGN
                     VQPGDGRRFKGRGPIQLTGRANYREFGRRVGIDLEGHPEIAALPSIGMLTACTYWTVK
                     GLNALADRDDLDGITRRINGGTNGLADRAVRLRAMKALLP"
BASE COUNT         1057 a         2217 c         2234 g         1031 t
ORIGIN      
        1 tgacgggtgg tgccggctat gatgagctct acggcggcct cggatcggat cgcatggatg
       61 gcggctcgac cggcgattac ctggagggtt tcgacgggaa cgacaccctg ttcggcggcg
      121 gtgcgaacga cttcatcacc ggcgataacg gtatggaccg ccttattggc ggctcgggcg
      181 atgatcgcct cttcgcgggc actgggaacg atctgctcat tggcggcacg ggtgtggacc
      241 tgctggcggg cgagcttggc gcggacagct atcgcttctc cgacggtcat accggctcca
      301 cagcggcctt ggccgacacg atcgagggct tcagccgcgg cgagggcgac gtcatcgacc
      361 tctcgcgcgt cgacgccgcg accggcgccg gcaacgacgc cttcagcttc atcggcgacg
      421 gcgccttctc cggcacggcc ggcgagctgc gctacgagcg gaacggcctc ggcgtaatgg
      481 tcgaagccga ccgcgacggc gacagcatgg ccgacttcta catccaggtc tcaggcccga
      541 tcgagctggt cgccagcgac ttcgtgctat gatgaaatga accgggcggc aacggcgcac
      601 ccgcgccgct gcccccccaa tcgaaacgaa acctgctggc gctggcgttc attctggccc
      661 gcaccagctg gtcgctcgcc atggcgttgc cggcgatcgc ggggggcgcg ctcgatggga
      721 cgcaggagcc gcttcttctc gcgctcctgc ccggtgcagc cgcggcgatc gcggcgatcc
      781 tgctcgctgc ctggacccgg tccgccttcg cgcctcggct ggtcctcctg atcgcctggt
      841 atgtgtatct ttcggcggcc tagcactccg cgctagccga tcttcggggc gaagcgctcg
      901 cgcatatagg cgatatattc cgcctcggag gtctcccgcc gcatcgcaag catcggcttg
      961 atgtcctcag tggcgatata acgcagctga tccgtgccgt cggtgactgc cctatggatg
     1021 acttcggcca cctcttcgga cgtggcgccg gcccgcgcct tgaccagccc ctggaatatc
     1081 gcgccggcac cgtcgaggaa cggccggtaa tccgagagcc cgccgacgag cgaggcctcc
     1141 tcgccgctgc gggcggtgaa gccggtgctg gtgacgcctc cgggctcgac gatcttcacc
     1201 ctgacgccga ggtcggccag ctcgtaggcc aaggattcgg aaaagccttc gagcgcgaat
     1261 ttgctcgacg tgtagagcga gatcaggggt agcgcgaagc cgccggcgcc tgagctgacg
     1321 ttggcgatca cgccggaccg gttggcgcgc aagtgcggga gcacggcccg gatcaccgcc
     1381 atcgcgccga acagattcac ctcgaactgc tcgcggatct tcgcccaggg cgtggcttcg
     1441 aagatgccca tgaggccgaa gccggcattg ttaacgaccg cgtcgatgcg gccgaagcgc
     1501 tcgatcgtct ccgcgacggc ggcttcgatg ctgggctggt cctcgacgtc gaggcgcgta
     1561 accagcacat tgtcgagccg ggccagcgcg gcgccggcct cgggccgtcg catcgtcgcc
     1621 gccacgttcc agccgcgctc cgcgaaaagc cgcgccgtcg cctcgccaaa gccggatgag
     1681 gcgcccgtga tcagaatggt gttcggcatc atcaattcct tcgccgttcg gatgcggctc
     1741 aagcggccac cgcggctagg cgaccgccgc caaagcgcct tccgccgccg cctcgatcag
     1801 cgctgccgtg ctttccggct tggcgacgaa gatggcgtgg cttccggcgg cttcggtgac
     1861 gttcgcgccg gcgcgggcgg ccatcgtccg ctgcgcctcg ggcgggatca tgcggtcttc
     1921 ggacgcgacc agataccagc tcggcttggt cttccacgcc ggctcgccga ccgtggcgct
     1981 ggccgccgcc acgccccagg gggcctgcgc gatcgccatg aattgggcct tggccgggtc
     2041 cacgtcggcc gcgaaggcgg cgggaaagcg gaccgggtcg acgaagagga agccgtcgct
     2101 gggcgggagc accggtgccg acggcgcgcc ggcgggcagc tgcccgatca ggtcgaggac
     2161 ggactggccg ctgtccggca cccagcccgc tatgtagacg agcgccttca ccttggggtc
     2221 gcggccggcc tcggtgatga tcgcgccgcc gtaggaatgg ccgacgagcg ccacgggacc
     2281 gtcttgggcc gcgatcgcgc gggcaacata agcgacgtcc tcggccagcg aggcggtcgg
     2341 atgctgcacc acggtgactt catacccctt ggcgctgagc aggcggtaaa cgccctccca
     2401 gccggacccg tccacgaacg cgccatggac cagcacgatg ttccggatcc tgcctggatc
     2461 cggtcgtcga tgggcgtcag gcggcatttc tcgtctcccc gatgcgcggc aagagcgcgt
     2521 tgtcggggcc cgtcctgcca cagcctgtcg ctccgccgcg gcgagctgat gctagccgtg
     2581 gtgagaatgc gtaagccgat cgggcgagcg tcgcttcgcg gcctgcagca ttaataatat
     2641 tagacattac cgtagaaaca cgtaggaagg tcgggcgcct tctccgaggc atcggccaga
     2701 ctcgcgcata actacttctc agacgagcgg tcattcagtc gttcaggcgg cttgcgtagc
     2761 cgtacctccg gtagctgaag gattgtgtat ggctgatctg atcgtgggcg acggcggctt
     2821 cggcacaatt caggctgcgg tcgacgcggc aagcgacggc gatacgatca ttgtcgcggc
     2881 aggaacctat gtcgaacaag tgatcgttca gaaccgaaac ggcctgacga tccgcgctgc
     2941 cgacggcgag caggtgacgc tgcgctcgcc cgccgacctg gtggagacgg cgcggtcgag
     3001 cagcgatcgc gagattcatt ccgtttttac agtcaaggac agttccgaga tcgtcctgga
     3061 aggcatcgac gttgacggcg aaggacgcgg gacaacggtc gacgagggcg gcggcgccgg
     3121 catcgccaat ttttacggga tcttctaccg gaacgcctcc ggcgggctga ccgacgtcga
     3181 tatcgtgggc gtgcgggacc cctatccggg cgggaccacc cctgggggcc agcccgaggt
     3241 cgacgccgtc cagcgcggca tcgccgtggt cgccgacaat gacagtctcc gcgccttcgc
     3301 catcaccggc gggacgatct ccgatttcca gaagcaggcc ggcctgttca gccgcgccga
     3361 cctggccatc accggcgtca ccgtcaccgg cggcggcgcg cagccggtca tggcgcagaa
     3421 cggcttttcc gtcaccaatt cgagcggcat cgtgtccggc aacacgttca gcgggattgg
     3481 ctatgccggc ccggccccgg cttattcggg tgcgatcctg gcttcgtcga acaccgacct
     3541 tgcgatcacc ggcaacacga tcaccggcgc caatggcgat tccacggccg ccaaggtggt
     3601 cggcatctgg gtctatgtcg cctcggccgc caataatggc gtcgagatca gcggaaacac
     3661 gatctccggc gtcgatgtcg gcatcaacgc ctccggcaat ttcggggcga acggcatcct
     3721 catccgggac aatgaggtga ccgacctcga tctcgccgat ccctatgcct acggcgtgaa
     3781 cttcgacccg ctggcgacgc tgcccaccgc cttcgaagtc gacggctccg cccgcagcga
     3841 tatcctgcgg ggcgccgccg gcggcgacac cctgtccggc ctcggcggca aggatgagat
     3901 tatcggctgc ggcggcgacg acctcatcga cggaggggcc ggcgacgatg tgctcgacgg
     3961 cgacgatggc gacgatcggc tggaaggcgg cgccggcgac gacgatctcg acggtggagc
     4021 cggaagcgac acggtgagct tcagcacggc catggccggc gtggaggtcg atcttgccgt
     4081 ggcggggccg cagaacaccg gcgacggcag cgacctcatc gtcaacatcg agaatgcgtt
     4141 ggggtcggag ttcgccgact atatcagcgg caatgccggc tccaatcacc tgcgcggcgg
     4201 cgccggcgcg gaccggcttt tcggccggtc ggaggcggac cgactgtccg gcgacggcgg
     4261 cgacgacatt ctcgacggag gcatcggcct cgatgagatg gccggcggcg ccggcgacga
     4321 cctctatgtc gcccgcgaga ccggcgatgt cgcgacggaa gcggccgagc agggacatga
     4381 caccgtcaag gccacggccg attacacgct cggcgaccat gtcgaggatc tttacctgca
     4441 gggtctggcg gtccgcggca ccggtaacgg gctggacaac cgcgtggtgg gcaccaacgg
     4501 cggcaatgtg ctggaggggc ttgccgggaa cgacatcctc tacggctata gcggcatgga
     4561 cgagatggac gggggcgagg gcgccgacac tttctatggc gggctcaaca acgatgccct
     4621 ggacggcggc tcgggcaatg accggctcaa cggcgaagct ggcgcggatt tcatcgaggg
     4681 cggcgacgac gacgaccatc tgttcggccg cgacggcgcc gacgagctcc tcggcgggca
     4741 ggggatggac cgcctcgagg gcggcgctca aaccgatatt ctgaccgggg gcgccggcct
     4801 cgacgtgttc gggttcggca atggcgacgt gccgggcaca cgcttcagcg ccgatcggat
     4861 caccgatttc agccaggccg agggagaccg cattcatcta cgccagcttg atgcgaacct
     4921 cggcatcgcg ggcgatcaga actttcactt tgtcggcgaa agccatttct ccggcaatgc
     4981 gggcgaggtg cgattcgacc attccggggg caatacttac gtccagggag acgtggacgg
     5041 cgacagggcc gccgacttct acgtccgggt cgacggaatt cattcgctcg tcgtgaccga
     5101 tttcgtgctc tgaccgggga gttttgaacg gtcgcaccct gtgcgggtgc cgagaagggc
     5161 atccggccga aagaggattt ccgaggtgtg gaggcccgag ccggaatcga accggcgtgc
     5221 aaggatttgc agtcctctgc gtaaccactc cgccatcggg ccgcgccgct ggaattaggc
     5281 ggttttttcc gggagagcaa ccgcgagttc gcacccgttt tcagcgagtt cgcaccgttc
     5341 ccgattcgtg cacgcacgag aaaagccccg cccgcccggg catcagccgg gcgggcgggc
     5401 ggtaaatcgt cccggaagag agcggccctt cggctattcg cgtagcgcct cgtgcagcag
     5461 ccaggcggcg agcaataacc ccatagcgag cagcaccgcc acccaccaca agcctcggat
     5521 cgcgcggcgc cgtccccggc gcagcgcccg gccggggctc atatccgctt ggcgccggcc
     5581 gccgcggcct ccgcctcgcg cgtcttctcg gtgatcagca tgatcccgat cgccccgaat
     5641 cgaaaggccg tgcacacttc ccgcgcgcgc gcgagcttgt cggccggcca gccggcggtg
     5701 agctcgtcga ccgccgccgt gaactcgccg cccgccatct cggccgccag accggcgagg
     5761 cagagcggca tcagcggctc gagcgcggcc ctgaggtcgt gatcggcgcc atagaccggc
     5821 agcgcgacgt cggcggccgc cagcggcgcc ggagcggccg ttgcgggcgc cgccgcgcgc
     5881 ttgatcgcgg atcccgccca ggccgagttg cccagcgcct cggcgacctt gcggctgccg
     5941 tccccacacc ccgtcaccag catcgccgcc agcagcgcca ccacccgaat ccgcatcgcc
     6001 cgtacctcca cgcaaaaagc ccgcccgggc agcgccaggg caggcggaaa actcgaaccg
     6061 gccgcccagc ggccagcggg ggcgcatatc cggaaaagac ggccgtcaga tcagggaagc
     6121 agcgctttca tcgcccgcag gcgcacggcg cggtcggcga ggccgttggt cccgccgttg
     6181 atgcgccgcg tgataccgtc caggtcgtcg cggtcggcga gcgcgttcag ccccttcacc
     6241 gtccaatagg tgcaggcggt gagcatgccg atcgacggca gcgccgcgat ctccggatgc
     6301 ccctcgaggt cgatgccgac gcgccggccg aactcgcggt aattggcgcg gccggtgagc
     6361 tggatcggcc cgcgcccctt gaagcgccgg ccgtcgcccg gctgcacgtt gccgaggtcg
     6421 cggcggccct cataggcctc gccgctggca atctcctcca tgtagcgaaa gccgccgctt
     6481 tcatggccga gttgcgcgag gaaatgggcg aagcggagcg gcgtcgcatc gatccccgc
//
LOCUS       DASVRK010000296         5883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_53345, whole genome shotgun sequence.
ACCESSION   DASVRK010000296 DASVRK010000000
VERSION     DASVRK010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53345"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VF589_12425"
     CDS             <1..1173
                     /locus_tag="VF589_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258427.1"
                     /translation="KPVTVTYKNCARDHDRPGFMVVTEAAAPSPQVLAMLARLNPVQR
                     AQLEALMARAASPCERGEPVAAGSADYRGSAAPGAVLARLGELVIEVGPKKAGAPVRL
                     LGMRTPDRLYRLDRGALVALAGHVTPKQNSLAVFKKFDRPVLMISGPVEVAEGVRGAG
                     NAVLTASVEHGYMGELTQETTIRTLFSSRSLPKGTTLYGIPMASRVVMTRNGVPVGDS
                     GVGRQPRADDLHLTWCVPVEEQGQWTATCLPTNGDSYTLLKGQRPAFEVRSLSYTAST
                     TTNPGAVPVIQKSGSFGRSLQHRFTIREVTPTRISLAQETLYGGVVVHSRPHELARLA
                     GKPSALFFGGGAVTFADAGAGKLLVKRHDAFEAGSDAMHAQSGVMSRISPPVITPR"
     gene            1229..1690
                     /locus_tag="VF589_12430"
     CDS             1229..1690
                     /locus_tag="VF589_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX8258428.1"
                     /translation="MGHGIAIAAALLLGAAAPAPRPEARSVDALAWMAGTWESGDRGR
                     WTEEHWTAPRGGMMMGLSRSGGANGVGEFEYIRIAPGEGGVPTYWASPGGGAATGFAL
                     VESGGQAAVFENRGHDYPQRSAYRRVGDTMTATISLADGGKPRSWRYRRAR"
     gene            1745..3448
                     /locus_tag="VF589_12435"
     CDS             1745..3448
                     /locus_tag="VF589_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8258429.1"
                     /translation="MKFPAAAALLLAGLALAASAPAAPAAPAAPAAPPQYGSGPIDPA
                     RLSEITRVLASDEYEGRAPGSAGETRTIAYLSEQFKKLGLEPGGENGGWTQAVPMIRT
                     QLKAPGSFSIEAKGGPLPVRFPSDIYLSTVRPIEQARIEAAPMVFVGYGVSAPERQWD
                     DFKGVDLKGKIAVFLVNDPDFEAAAADPVANRFGGRAMTYYGRWSYKFEEAARRGAVA
                     ALVVHETEGAGYGWSTVEAPGGENYNIVQAAGAQQPVLLQGWIQRPLAAELLKRAGHD
                     FEAVKRSARTASFKPIDLGARFSADVGVTLSRIQSHNVFAKITGSKRPEETVIFAAHW
                     DSFGTGAPDAQGRRVRAGAVDDALGVAGVIEIARAFKAGPRPERTLVFAAWTAEERGL
                     LGAEHYGAHPLYPHETTVANLTLDVLQTAGRARDVILIGAGQNEMEDELARAAARQGR
                     TVTPDAAPERGLFYRADHFALARRGVPGLLLMALGGGVDLVEGGRAAGEQWVADYTAN
                     CYHKPCDAWSADWDLRGAAQDVALAYEIGRDLANSNRWPGWKAGSEFRDLRAKSAAAR
                     R"
     gene            complement(3465..4187)
                     /locus_tag="VF589_12440"
     CDS             complement(3465..4187)
                     /locus_tag="VF589_12440"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8258430.1"
                     /translation="MIGMYYYIALGLFAAFGALDLVARARAFPDVAHWRLKGAAFTLL
                     YFAAATAGPLLWDGAIGRYRLFDASALPLALQVAGGLLLLELGIYAWHRSMHRVPLLW
                     RWFHQLHHSAERVDIWGAFYFSPLDMIGWALLGSLVLVGGFGIGAEAAILVGVLVTFL
                     SMFQHANIRTPHWLGYFIVRPEGHSLHHERGVHGYNYCDLPLWDMLFGTFRNPRAWNG
                     EAGFWNGASRRVGAMLLGKDVA"
     gene            4693..>5883
                     /locus_tag="VF589_12445"
     CDS             4693..>5883
                     /locus_tag="VF589_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8258431.1"
                     /translation="MRMARLAKERKRSGFAFGTSLIALLAMSSAAAAQSGGGAAGAGP
                     GEPEDEQEIIVTATKRNSTVQDVPFSINAQSEEDIQKSGATTIEDLSRNVAGLTVQNL
                     GPGQSQVAVRGVSAGQIVRDQPGVKEQVGVYLDESVISLSLFTPDIDLFDLNRVETLR
                     GPQGTLFGSGSVGGTIRYITNQPRTDRVEGTVEGNLNLIDGGSIGGHIKGAINVPVSE
                     SVAIRAVGYHTEYAGFIDAFGPGGGEDVNDGRRTGGRVAILFEPTPDISITPRVLYQE
                     IRANGFNRQETYNLFANPYTDASVPGGRRPITFDERQQYLLLEEAFEDDILLADMTAS
                     FGFGGVELTSVTSYIDRDILVSRDASALTGSVSVDLGFPRRAVLLPSNLRDNTTLEQF
                     TQELR"
BASE COUNT          951 a         2015 c         2093 g          824 t
ORIGIN      
        1 aagccggtga cggtgaccta caagaattgc gccagggacc atgaccgccc ggggttcatg
       61 gtcgtgaccg aggctgccgc cccgtcgccc caggtcctgg cgatgctggc caggctcaat
      121 cccgtgcagc gcgcgcagct cgaggcgctg atggccaggg ccgcgtcgcc ctgcgagcgc
      181 ggggagccgg tcgcggcggg cagcgccgac tatcgcggga gcgcggctcc gggcgccgtg
      241 ctggcacggc tcggcgagct ggtgatcgaa gtggggccga agaaagcggg cgcgccggtc
      301 cggctgctcg gcatgcgcac tcccgaccgc ctctaccggc tcgatcgcgg cgcgctggtg
      361 gcgctggccg gccatgtgac gccgaagcag aacagccttg ccgtgttcaa gaagttcgac
      421 cggccggtgc tgatgatctc cggtccggtc gaggtggccg aaggcgtgcg gggcgccggc
      481 aatgccgtgc tgaccgccag cgtcgaacat ggctatatgg gcgagctcac gcaggagacg
      541 acgatacgga ccctgttctc gagccgctcg ctgcccaaag gcacgacgct ctatggaata
      601 ccgatggcca gccgggtggt gatgacgcgg aacggcgttc cggtaggcga ttccggggtc
      661 ggccggcagc cccgcgccga cgatctccac ctgacatggt gcgtccccgt cgaggagcag
      721 ggccagtgga ccgccacttg cctcccaacc aacggcgact cctacacttt gctcaaaggg
      781 caacggccgg cgttcgaggt tcgcagcctc tcctatacgg cgagcacgac caccaatccg
      841 ggggcggtgc cggtcatcca gaaaagcggc agcttcggcc ggtcgctcca gcaccgcttc
      901 accatcaggg aggtcacgcc gacccggatc agcctggccc aggagacgct ctacggcggg
      961 gtcgtggtcc acagcaggcc gcacgagctg gcgcggctcg ccggcaagcc gagcgcgctc
     1021 ttcttcggcg gcggcgccgt caccttcgcc gacgcgggcg cgggaaaatt gctggtgaag
     1081 cgccatgacg cgttcgaagc cggctccgac gcgatgcacg cgcagagcgg ggtcatgagc
     1141 cgcataagtc cgccggtcat aacgcctcgc tagccgcggg ccatggccgg cgcttcgtgc
     1201 tatgcccgtc gggcaaagga gacgcgaagt gggccatgga atcgcgatcg cggcggcgct
     1261 gctcttaggg gcggcggcgc cggcgccgcg gccggaggcg cggagcgtcg acgcactcgc
     1321 ttggatggcc gggacctggg aaagcggcga ccggggccgc tggaccgagg agcattggac
     1381 ggcgccgcgc ggcggcatga tgatgggcct tagccgctcc ggcggcgcca acggcgtcgg
     1441 cgaatttgaa tatatcagga tcgcgcccgg cgaaggcggc gtgccgacct attgggcctc
     1501 gcccggcggc ggcgcggcga ccggcttcgc tttggtcgaa agcgggggcc aagcggcggt
     1561 gttcgagaat cgcggccacg attatccgca gcgcagcgcc tatcgccgcg tgggcgacac
     1621 catgaccgcc accatctcgc tggccgacgg cggcaagcct cgcagctggc gctaccgccg
     1681 cgcgcgctga gccgggcgaa ccaatgcgcg cgccgcccgt tggagcggca tcggaggacc
     1741 gcccatgaaa ttcccagccg cggctgcttt gctcctcgcc ggcctcgcgc tcgcagcctc
     1801 cgccccggcg gcgccggccg ccccggccgc gcccgccgcg ccgccccaat atggctccgg
     1861 cccgatcgat ccggcgcggc tgtcggagat cacgcgggtg ctcgcctccg acgaatatga
     1921 aggccgcgcc ccgggaagcg ccggcgagac caggacgatc gcctatctca gcgagcaatt
     1981 caaaaagctc ggcctcgagc cgggcggcga gaatggcggg tggacgcagg cggtgccgat
     2041 gatccgcacg cagctcaagg cgcccgggtc gttttcgatc gaggccaagg gcggccccct
     2101 ccccgtccgc ttcccgagcg acatctacct cagcaccgtc cgcccgatcg agcaggccag
     2161 gatcgaagcc gcgccgatgg tgttcgtcgg ctacggcgtg tcggcgcccg agcggcagtg
     2221 ggacgatttc aagggcgtcg acctcaaggg caagatcgcc gtcttcctgg tcaacgaccc
     2281 ggatttcgaa gcggcggcgg ccgacccggt cgccaatcgc ttcggggggc gggcgatgac
     2341 ctattatggc cgctggtcct acaagttcga ggaggcggcg cggcgcggcg cggtcgcggc
     2401 cctggtcgtg cacgagaccg aaggcgccgg ctatggctgg agcacggtcg aggcgccggg
     2461 cggcgagaat tacaatatcg tccaggccgc gggcgcgcag cagccggtgc tgctgcaggg
     2521 ctggatccag cgcccgctcg ccgccgaatt gctgaagcgc gccggccacg atttcgaggc
     2581 ggtgaaacgg agcgcgcgca ccgcttcgtt caagccgatc gaccttggcg cgcgtttctc
     2641 cgccgacgtc ggcgtcaccc tgtcgcgcat ccagagccac aacgtcttcg ccaagatcac
     2701 gggcagcaag cggcccgagg agacggtgat tttcgccgcc cattgggatt cgttcggcac
     2761 cggcgcgccc gatgcccaag ggcggcgtgt gcgcgccggc gcggtcgacg atgcgctcgg
     2821 cgtggccggc gtgatcgaga tcgcccgcgc cttcaaggcc gggccgcggc ccgagcggac
     2881 gttggtgttc gccgcctgga cggcggagga gcgcggcctg ctcggcgccg aacattatgg
     2941 cgcgcacccg ctctacccgc acgagacgac ggtcgcgaac ctcacgctcg acgtgctcca
     3001 gaccgccggg cgggcgcgcg acgtgatcct gatcggcgcc ggccagaacg agatggagga
     3061 cgagctcgcc cgcgccgccg cgcgccaggg ccgcaccgtc acgcccgacg cggcgccgga
     3121 gcgcggcctg ttctaccggg ccgaccattt cgcgctggcg cggcgcggcg tgcccgggct
     3181 gctgctgatg gcgctgggcg gcggcgtcga cctggtcgaa ggcgggcgcg ccgccggcga
     3241 gcaatgggtc gccgattata ccgccaattg ctaccacaag ccgtgcgacg cctggagcgc
     3301 ggactgggac ttgcgcggcg cggcgcagga cgtggcgctc gcctacgaga tcggccgcga
     3361 cctcgccaac tccaaccgct ggcccggctg gaaagcgggc tcggaattcc gcgacctgcg
     3421 cgcgaagagc gccgccgccc gccgctagcg ccgccggagg cggttcaggc gacgtccttg
     3481 ccgagcagca tcgcgccgac acggcgcgag gcgccgttcc agaagccggc ctcgccgttc
     3541 cacgcccgcg ggttgcggaa ggtgccgaac agcatgtccc acagcggcag gtcgcaataa
     3601 ttgtagccgt ggacgccgcg ctcatggtgg agcgagtggc cttccgggcg gacgatgaaa
     3661 tagccgagcc agtggggcgt gcggatgttg gcgtgctgga acatcgacag gaaggtgacc
     3721 agcacgccaa ccaggatcgc cgcctcggcg ccgatgccga agccgccgac cagcaccagg
     3781 ctgccgagca gggcccagcc gatcatgtcg agcggcgaga agtaaaaggc gccccagatg
     3841 tcgacccgct cggcgctgtg gtgcagctgg tggaaccagc gccacagcaa cggcacccgg
     3901 tgcatcgagc ggtgccaggc gtagatgccg agctcgagca gcagcagccc gccggcgacc
     3961 tgcagcgcca gcggcagcgc gctggcgtcg aacagccggt agcggccgat cgcgccgtcc
     4021 cacagcagcg gcccggcggt ggcggcggcg aaatagagca aggtgaaggc cgcgcccttc
     4081 agccgccaat gagcgacgtc gggaaaggcg cgggcgcggg cgacgaggtc gagcgcgccg
     4141 aacgccgcga acaggccgag cgcgatataa tagtacatgc cgatcatgat gccctccgtg
     4201 tcgaatgagc gggcggcgta gcgggcggac gtgggcggga gcgtttgagg cagcgttgga
     4261 ttttgggagt cgtgcgtcct tcgagacgcc gcttcgactt cgctcagcgg gggcaggttg
     4321 ggcgctaccc gcgcccctgc gaaggcaggg gcccagacca ggcgcagcct gtcaaacgag
     4381 ggtagaggcc gcgccagtcc gggttcatct gtgccgttga ggccgctccc attcgtgtga
     4441 acatatcgcc cttcatccgg ggaggtcggc cgggccggcg ctaagcgccg ggtctggacc
     4501 cctgccttcg caggggaacg ggtgcggcga ccccatccgc agctcgacca atctagctcc
     4561 ctccccctcc ccctttcctg tctcccatct acaacagcgg cagggtttcg ggggcgggcg
     4621 gggcggcatc acttgtgtca attatgcgtc acccataaca tcgccgccgg aacgacttgg
     4681 ggaggaacgg acatgcgcat ggctcgtctg gctaaagagc ggaaaagaag cggttttgct
     4741 ttcggcactt cgctgatcgc cttgctcgcc atgtcgtcgg ccgcggccgc gcagagcggc
     4801 ggcggggccg cgggggccgg cccgggcgaa ccggaggacg agcaggagat catcgtcacc
     4861 gccaccaaac gcaattcgac ggtgcaggac gtgcccttct cgatcaacgc gcagagcgag
     4921 gaagacatcc agaaatcggg cgcgaccacg atcgaggacc tgtcgcgcaa cgtcgccggc
     4981 cttaccgtcc agaatcttgg gccggggcag agccaggtcg cggtgcgcgg cgtttcggcc
     5041 ggccagatcg tccgcgacca gccgggcgtc aaggagcagg tcggcgtcta tctcgacgaa
     5101 agcgtgatct cgctgtcgct gttcacgccc gatatcgacc tgttcgattt gaaccgcgtc
     5161 gagaccctgc gcgggccgca gggcaccttg ttcggatcgg gatcggtcgg cggcacgatc
     5221 cgctacatca ccaaccagcc gcgcaccgac cgcgtcgaag gcacggtcga gggcaatctg
     5281 aacctgatcg acggcggctc gatcggcggc cacatcaagg gcgcgatcaa cgtaccggtg
     5341 tccgaaagcg tcgcgatccg cgcggtcggc tatcataccg aatatgccgg cttcatcgac
     5401 gcgttcgggc cgggcggcgg cgaggacgtc aatgacgggc ggcgcaccgg cgggcgcgtc
     5461 gcgatcctgt tcgagccgac gcccgacatc tcgatcacgc cgcgcgtcct ctaccaggag
     5521 atccgcgcca acggcttcaa ccggcaggag acctacaacc tcttcgccaa cccctatacc
     5581 gatgcctcgg tccccggcgg gcggcggccg atcacgttcg acgagcgcca gcaatatctg
     5641 ctgctcgaag aggcgttcga ggacgacata ttgctcgccg acatgaccgc gagcttcggc
     5701 ttcggcggcg tcgagctgac gtcggtgacg agctatatcg accgcgacat cctggtcagc
     5761 cgcgacgcca gcgcgctgac cggcagcgtc tcggtcgatc tcggcttccc gcgccgtgcg
     5821 gtgctgctgc cgtccaacct gcgcgacaac acgacgctcg agcaattcac ccaggagctc
     5881 cgg
//
LOCUS       DASVRK010000297         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_553041, whole genome shotgun sequence.
ACCESSION   DASVRK010000297 DASVRK010000000
VERSION     DASVRK010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553041"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2533
                     /locus_tag="VF589_12450"
     CDS             <1..2533
                     /locus_tag="VF589_12450"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8258432.1"
                     /translation="DGGGNDTYNFDRYTWNLKVDLQPGHWSSGGQTAELAYGQDAPGT
                     VANALLYQGDERSLIENAIGGTGDDVITGNFAANRLAGGGGIDLLQGLAGDDMLIAGA
                     GNRRWELAPGPELDRSSRDLALSLDGVMKINPTSWVERSLAVPYARVEGASTGAVEYY
                     RFTVREPGRISIDIDQAEGSLAIELSDGSGRRLASNSDGSVTDPGDIGATQPYIAYDA
                     GPGHYFIKVSYAADAEPFTWVMNVSVPGAAVEQITGSRLEGGAGNDGLYGEAADDMLL
                     GGSEADTLDGGGGLDTLNGGDGADILMGRMDGDVLIGGAGADTASYKLAASGVAVDLT
                     LTSAESVAAGADWMQGVENLTGSGFDDQLTGDRGANVLDGGAGADVLAGGSRNDVYVV
                     DQAGDRVIELAGDDKDQVLSSISYALGTHVEQLTLTGSAAIGGRGNDAANAIVGNAAA
                     NLLQAYGGEDVLDGRGGADTMKGGQGNDLYHVDQAGDRVVEAAGEGEDTIFSNVSFRL
                     GDHVEILGLTGEGDIGGTGNALANRLSGNDGDNRLDGGAGADLLAGGAGNDVYVVERG
                     TDRVSESAASIGIDRVESSVSFTLTDGVENLTLTGNAAIGGTGNAAANLLTGNNAANR
                     LDGGGGADRMIGGGGNDVYSVDAAGDAVIEASATGGRDMVRSAISYTIGLKLDNLTLI
                     GSEAIDGTGNRFDNLLTGNAGANALDGEAGADKLNGGAGHDGLAGGRGSDVLTGGTGR
                     DSFVFDSFLGGGEVDTIADFSAADDSFRLDGSVFAAFAAEGRIAGGAFQTGRAALDGS
                     DRILYDGASGKIFYDADGSGDIAAILFAKVDAGSALTAADFIVYG"
BASE COUNT          461 a          876 c          971 g          354 t
ORIGIN      
        1 ggacgggggc gggaacgaca cctacaattt cgaccgctat acgtggaacc tgaaagtgga
       61 tctccagccg gggcattgga gctcgggcgg tcaaacagcc gaactcgctt atggccagga
      121 cgcacccgga accgtcgcca acgccttgct ctaccagggc gacgaacggt cgctgatcga
      181 aaatgcgatc ggcgggaccg gggacgatgt catcaccggc aatttcgccg ccaaccggct
      241 cgcgggcggc ggcgggatcg accttcttca gggcctggct ggggacgata tgctgatcgc
      301 cggcgcgggc aacaggcgct gggaactggc cccggggccg gagctcgacc ggagcagccg
      361 cgatctcgcg ctgtcgctgg acggcgtgat gaagatcaat ccgacctcct gggtcgaacg
      421 ctcgctggcc gttccctacg ccagggtaga gggcgcttcg accggcgcgg tggaatatta
      481 ccggttcacc gtgcgcgagc cgggcaggat ctcgatcgac atcgaccagg ccgagggcag
      541 cctcgccatc gagctcagcg acggatcggg gcggcggctg gccagcaatt ccgacggtag
      601 cgtcacggac ccgggcgata tcggcgccac gcagccgtac atcgcctacg acgccggccc
      661 cggccattat ttcatcaagg tcagctacgc cgccgacgcg gagcccttca cttgggtgat
      721 gaacgtcagc gtacccggag cggcggtcga acagatcacc ggatcgcggc tggaaggcgg
      781 ggcgggcaac gacgggctct atggcgaggc agcggacgac atgctgctgg gcggcagcga
      841 ggcggacacg ctcgacggcg gcggcggcct cgacaccctc aatggcggcg acggcgcgga
      901 catattgatg ggccggatgg acggcgacgt gctgatcggc ggcgccggcg cggacacggc
      961 gagctataag ctggcggcga gcggcgtcgc cgtggacctg acgctgacct ccgccgagag
     1021 cgtggcggcc ggcgccgact ggatgcaggg cgtcgagaat ttgaccggct ccggcttcga
     1081 cgaccagctg accggcgacc ggggcgccaa tgtgctcgac ggcggcgccg gcgcggacgt
     1141 gctggcgggc ggaagccgca acgacgtcta tgtcgtcgac caggccggcg accgcgtgat
     1201 cgagctcgcc ggcgacgata aggaccaggt gttgagctcg atcagctacg cgctcgggac
     1261 ccatgtcgag cagctgacgc tgaccggcag cgccgccatc ggcggccgcg gcaacgatgc
     1321 ggccaacgcg atcgtcggca acgctgcggc caacctgctg caagcctatg gcggcgagga
     1381 tgtgctcgac ggacgcggcg gcgccgacac gatgaagggc ggccagggca acgatctcta
     1441 tcatgtcgac caggccggcg accgcgtcgt cgaagcggcc ggcgagggcg aggacacgat
     1501 cttcagcaac gtcagcttca ggctcggcga ccatgtcgag atcctcgggc tgaccgggga
     1561 gggcgatatc ggcggcaccg gcaacgcgct cgccaaccgc ctctccggca atgacggcga
     1621 caatcgcctc gacggaggcg ccggcgccga cctgctcgcc gggggcgccg gcaacgacgt
     1681 ctacgtggtc gagcgcggga ccgaccgggt ctccgaatcg gcggcgagca tcggcatcga
     1741 ccgggtcgaa agctcggtca gcttcacgct gacggacggg gtcgagaatc tgacgctgac
     1801 cggcaatgcc gcgatcggcg gcaccggcaa cgccgccgcc aacctcctca ccggcaacaa
     1861 tgccgccaac cggctcgacg gcggcggcgg cgccgaccgg atgatcggcg gcggcggcaa
     1921 tgacgtctat agtgtcgatg cggccgggga cgcggtgatc gaggccagcg cgaccggcgg
     1981 ccgcgacatg gtgagaagcg cgatcagcta cacgatcggg ctcaagctcg acaatctgac
     2041 cctgatcgga agcgaggcga tcgacggcac cggcaaccgg ttcgacaatc tgctgaccgg
     2101 caatgccggc gccaacgcgc tggacggcga ggccggcgcc gacaagctca acggcggcgc
     2161 gggccatgac ggcctggcgg gcgggcgcgg cagcgacgtg ctgaccggcg ggaccggccg
     2221 cgacagcttc gtcttcgaca gcttcctcgg cggcggcgag gtcgacacca ttgccgattt
     2281 cagcgcggcc gacgacagct tcaggctcga cggcagcgtg tttgccgcct tcgcggcgga
     2341 ggggcggatc gccgggggcg cgttccagac cgggcgcgcg gcattggatg gatcggaccg
     2401 gatcctgtac gacggcgcca gcggcaagat cttctacgac gcggacggca gcggcgacat
     2461 cgcggccatc ttgttcgcga aggtcgacgc cggcagcgcg ctgaccgcgg ccgacttcat
     2521 cgtctacggc tgatccggcc gcccgagttc atccgctcgg tggcgccgag ctttcagagc
     2581 cgcttcgcct atccggcctt catcgggcga agagtctgga cccctgcctt cgcaggggaa
     2641 caggtgcgct cgcaggggaa ca
//
LOCUS       DASVRK010000298         7920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_496095, whole genome shotgun sequence.
ACCESSION   DASVRK010000298 DASVRK010000000
VERSION     DASVRK010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496095"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /gene="katG"
                     /locus_tag="VF589_12455"
     CDS             complement(<1..1760)
                     /gene="katG"
                     /locus_tag="VF589_12455"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX8258433.1"
                     /translation="MDAKTGESMGCPVDRPSTVRSLLGRTNRDWWPEALPLEVLNQGG
                     HSPDPMGEGFDYAKAFNALDYAALKADLTALMTESQPWWPADYGHYGPLFIRMAWHAA
                     GTYRTADGRGGANSGQQRFAPLNSWPDNGNLDKARRLLWPIKRKYGKNISWADLFILA
                     GNVAIESMGGPVLGFGGGRQDVFEPERDIYWGAEEKWVNEGSETRIDPERQKELEHPL
                     AAIQMGLIYVNPEGPGGKPDPMGSARDMKTTFERMAMNHEETVALTAGGHTFGKAHGA
                     GDPSLVDAAPEAADIALQGLGWASAHESGFGEHTITSGIEGSWVNTPTQWSENYFRLL
                     LDYEYELVRSPAGAQQWQPINQREEDMAPAAHDPSKRVPTMMTTADMALKVDPEFRAI
                     SERFRTDHEAFKDAWARAWFKLTHRDMGPRVRYLGPEVPREDMIWQDPVPAGTTPSDA
                     DVAAFKDQILGAGFSVGQLVKTAWASASTYRRSDHRGGANGARLRLEPQRGWAANEPE
                     ELTGILARIDELRGSLSMADAIVLAGTAAVEKAARDAGSNVEVPFQGGRGDATQDWTD
                     VDSFVVMDPAADGFRNYLKT"
     gene            complement(2089..2319)
                     /locus_tag="VF589_12460"
     CDS             complement(2089..2319)
                     /locus_tag="VF589_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258434.1"
                     /translation="MSEPITYLAMAAFGLVAVSIAVFAGLRGWQGWLDLKRMELSGNH
                     QQGRSPAFERIEMADLKERIRKLEAIASCVDL"
     gene            complement(2350..2697)
                     /locus_tag="VF589_12465"
     CDS             complement(2350..2697)
                     /locus_tag="VF589_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX8258435.1"
                     /translation="MKKKFYLDKRNGKFLGVCSGIANFFGIDATFVRVGAVVITLLGA
                     APWTFIAYALAGWLAKPKPHGGYDADDVRAVRSSTHDVRASMRDIDRRMAEVETYVTS
                     SNTSLAREIDQLR"
     gene            complement(2724..3392)
                     /gene="pspA"
                     /locus_tag="VF589_12470"
     CDS             complement(2724..3392)
                     /gene="pspA"
                     /locus_tag="VF589_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEX8258436.1"
                     /translation="MSIFSRTRDIVAANVNDLLDKAEDPAKMIRMIVMEMEETLVEVR
                     TSAARSIADQKEMRRQIARLDDLQQSWTEKAELALSKDREDLAKAALVERQKVADLAD
                     GLGAEIAMLEDGLRASEDDIARLQAKLRDARTRQAAIQNRMESANNRIRLREMYAGSR
                     VDEAFSRFDLLEKRVDLAEGRADALALAAPIVLEHEIAALRTSDKAEAELAAMKASRG
                     MALI"
     gene            complement(3505..3666)
                     /locus_tag="VF589_12475"
     CDS             complement(3505..3666)
                     /locus_tag="VF589_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258437.1"
                     /translation="MFDFGKFQQVAAAALGALILTAVSVGAAVGPARAIETAPIAYAQ
                     VEGADQAHA"
     gene            complement(3905..4162)
                     /locus_tag="VF589_12480"
     CDS             complement(3905..4162)
                     /locus_tag="VF589_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258438.1"
                     /translation="MLQKFLRLFIIKTRFEAFAVIYAIAVGAVSRGYHYLDIYPGPLG
                     WVFFAACTGAVFMAGAKILDATAPRRRPGTDRRLGDRRAKA"
     gene            4368..5723
                     /locus_tag="VF589_12485"
     CDS             4368..5723
                     /locus_tag="VF589_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258439.1"
                     /translation="MRSHEEIVTEVAGRSPACAKVGVFDIDGIFRGKYMAVDKLGSTL
                     AKGFGFCDVIFGWDSNDQLLDNLNFTGWHSAYPDAEARLLPETLRAMPFEDGMPLILG
                     EFSGRAEAVCPRGTLRRILDRAAGLGFKVKASAEFEFFVFEETPQSVRDKGYRNLKSI
                     TPGYFGYSMLRSGVHSDFYRELWSTCDAMRIELEGLHTETGPGVIEAAIKVDDALEAA
                     DKAALFKTIAKILAQRRGWMATFMAKWSLDWPGQSGHLHMSLTDAETGEPIFYEEGAR
                     HGMSDRLRWFVGGQQALMPELLAMLAPTVNAYTRLVPGYWAPTAATWGYENRTCALRV
                     IGGSAHSQRVECRIGAADINPYVALAAAIGSGLWGIEHKIEPDEPIVGNAYEAEMPVE
                     RSLPSTLWDAAQRLRGSAAARDLFGSEFVDHYASTREWEEREARKAVTDWQLARYFEI
                     I"
     gene            5737..7125
                     /locus_tag="VF589_12490"
     CDS             5737..7125
                     /locus_tag="VF589_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8258440.1"
                     /translation="MSDDLVCISPVDGCEYARRALTPPAEVDRALARARAAQREWARA
                     PLAERCAVALRFLDAMERLNAEIVPELAWQMGRPVRYQGELRSLVDRVKGLVAVAEDA
                     LAPDEPEPGGGARRYVTRVPAGLVLVIAPWNYPFLTAANTIVPALVAGNAVVLKHASQ
                     TLLAGERFAQALQLAGLPQGLFHNLFLSHEETARLLGSGLVDHATFTGSVEGGRAIER
                     AAAGSFTTLTLELGGKDPAYVRADADIAGAVESLVDGSFYNSGQSCCGVERIYVDEAV
                     WQPFLDGFVAATQQYRLGDPLDRETSLGPMSSARLAGQVRGQVDEAVAAGAEAHIERQ
                     LFRADAAGTAYLMPQVLTGVDHEMAVMREETFGPVVGIMSVKSDDEAVRLMNDSRYGL
                     TASIWTRDAAAAERIAPEVQAGTVFMNRCDYLDPLLAWTGVKESGRGASLGRYGYEGV
                     TRPKSYHLRSGA"
     gene            7122..>7920
                     /locus_tag="VF589_12495"
     CDS             7122..>7920
                     /locus_tag="VF589_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8258441.1"
                     /translation="MTLPSANWNYPTAIRFGAGRIAELAELCRELGIERPLLVTDPGL
                     AALPMIAAAVEANGAAGVPTGLFSEVRGNPVGGNVEAGVVVYAAGGHDGVIAFGGGSA
                     LDAGKAIAFMSGQSRPIWDFEDKADWWTRADPAGIAPVIAVPTTAGTGSEVGRASVIL
                     DEDEGVKKIIFHPKMMPAAVILDPELTVGLPPHITAATGMDALSHCIEAYCAPGFHPM
                     ADGIAAEGVRLIHDWLPRAVADGTDIAARGHMLIAASMGATAFQKGLG"
BASE COUNT         1191 a         2516 c         2869 g         1344 t
ORIGIN      
        1 ttggtcttca gataattgcg gaagccgtcc gcggccggat ccatcacgac gaagctgtcg
       61 acgtcggtcc aatcctgggt cgcatcgccg cgcccgccct ggaacgggac ctcgacgttg
      121 gagccggcat cccgcgccgc cttttcgacc gccgcggtgc cggcaagcac gatcgcgtcg
      181 gccatggaca gggagccgcg cagttcgtcg atccgggcga gaatgccggt cagctcttcc
      241 ggctcgttgg ccgcccagcc gcgttgcggc tcgagccgca ggcgcgcgcc gttggcgccg
      301 ccgcgatggt cggagcggcg ataggtcgag gccgacgccc aggcggtctt gaccagctgg
      361 ccgacgctga agccggcgcc gagaatctgg tccttgaacg cggcgacgtc ggcgtcgctc
      421 ggcgtggttc cagccgggac cgggtcctgc cagatcatgt cctcccgcgg cacttccggc
      481 ccgaggtagc ggacccttgg ccccatgtcg cggtgggtga gcttgaacca ggcgcgcgcc
      541 caggcgtcct tgaacgcctc atggtcggtg cggaaccgct cggagatggc gcggaattcg
      601 gggtcgacct tcagcgccat gtcggcggta gtcatcatcg tcggcacgcg cttcgacgga
      661 tcgtgcgcgg ccggcgccat atcctcttcg cgctgattga tcggctgcca ctgctgcgca
      721 ccggcgggcg agcggacgag ctcatattca taatcgagca acagccggaa gtaattttcc
      781 gaccattggg tgggcgtgtt cacccagctg ccttcgatgc cgctggtgat ggtgtgttcg
      841 ccaaagccgc tttcatgtgc gctcgcccag cccaggccct gcagcgcgat gtcggccgcc
      901 tcgggcgcgg cgtcgaccag gctcgggtcg cccgcgccat gcgccttgcc gaaggtgtgg
      961 ccgccggcgg tgagcgcgac cgtctcctca tggttcatcg ccatgcgctc gaaggtagtc
     1021 ttcatgtcgc gcgccgatcc catcgggtcg ggcttgccgc cggggccttc cggattgacg
     1081 tagatgaggc ccatctggat cgcggcgagc gggtgctcga gctccttctg gcgctcggga
     1141 tcgatgcgcg tttccgagcc ctcgttgacc cacttctcct cggcgcccca atagatgtcg
     1201 cgctccggct cgaacacgtc ctggcggccg ccgccgaagc cgaggaccgg accgcccatc
     1261 gattcgatcg cgacgttgcc ggccaggatg aagaggtcgg cccagctgat attcttgccg
     1321 tatttgcgct tgatcggcca cagcaggcgc cgcgccttgt cgagattgcc gttgtcgggc
     1381 cagctattga gcggggcgaa gcgctgctgg ccgctattgg cgccgccgcg tccgtcggcg
     1441 gtgcgatagg tgccggcggc gtgccaggcc atgcggatga acagcggccc gtaatggccg
     1501 taatcggccg gccaccaggg ctggctctcg gtcatcaaag cggtgaggtc ggccttgagc
     1561 gccgcatagt cgagcgcgtt gaacgccttg gcataatcga agccctcgcc catcggatcg
     1621 ggcgagtggc cgccctgatt gaggacttcc agcggcagcg cttcgggcca ccagtcgcga
     1681 ttggtgcggc cgagcaggga gcggacggtg cttggccggt ccaccggaca ccccatcgat
     1741 tcgccagtct ttgcgtccat attgcctctc cggattatat ctgaattccg gtgaggcact
     1801 acgccggttg ggcccgagtc ggaagcggct aatcggcatg acactgatcg gaaaaaccaa
     1861 ttgggaggtg cgggcggcgc cgactggacg ggcttcaaac gtgatccggg ctaagcgtcg
     1921 tcccagcgaa agctgggatc tccctttcct ttcaggccga gccgcccgca cgtggaaagg
     1981 gagaagtgag attccagctt tcgctggaat gacggtgaag tgctggaatg acgggggcgc
     2041 gctgggatga aggcttgtgt cccggccctc cccgtcacgc ccctcccctt aaaggtccac
     2101 acaggaggcg atggcctcga gcttgcggat gcgttccttg aggtcggcca tctcgatccg
     2161 ctcgaaggcg ggcgagcggc cctgctggtg gttgccgctg agctccatcc gcttgagatc
     2221 gagccagccc tgccagccgc gcaggccggc gaagaccgcg atgctgaccg cgaccagtcc
     2281 gaaggcggcc atcgcgaggt aagtgatcgg ttcgctcatt gtcttatcct cccttccctg
     2341 cccccgcgcc tagcgaagct ggtcgatttc gcgggcgagg ctggtgttcg agctggtgac
     2401 gtaagtttcg acttcggcca tgcggcggtc gatgtcgcgc atgctggcgc ggacatcgtg
     2461 ggtcgagctg cgcaccgcgc ggacgtcgtc ggcatcgtag ccgccatggg gcttcggctt
     2521 ggcgagccag ccggcgagcg cataggcgat gaacgtccag ggcgcggcgc cgagcagggt
     2581 gatgacgacg gcgccgacgc ggacgaaggt ggcgtcgatg ccgaagaaat tggcgatgcc
     2641 ggagcacacg cccaggaact tgccgttgcg cttgtcgagg tagaatttct ttttcatgtc
     2701 ccgtgtcctt ccaatcagag cgttcaaatc agcgccatgc cgcgcgatgc cttcatcgcg
     2761 gcgagctcgg cttcggcctt gtcggaggtc cggagcgcgg cgatttcatg ttcgaggacg
     2821 atcggcgcgg cgagcgcgag cgcgtcggcc cggccttcgg cgagatcgac tctcttttcg
     2881 agcaggtcga agcggctgaa cgcctcgtcg accctggagc cggcatacat ttcgcgcagc
     2941 cggatccggt tgttggcgct ttccatccgg ttctggatgg cggcctggcg ggtgcgggcg
     3001 tcgcggagct tggcctgcag ccgggcgata tcgtcttcgg aggcacggag gccgtcttcg
     3061 agcatggcga tctcggcgcc gaggccatcg gccaggtcgg cgaccttctg ccgttcaacg
     3121 agggcggcct tggcgagatc ctcgcggtcc ttggaaaggg cgagctcggc cttttcggtc
     3181 cagctctgct gaaggtcgtc gagcctggcg atctgccggc gcatctcctt ctggtcggcg
     3241 atcgagcggg cggccgaggt gcggacttcg accagggtct cctccatctc catgacgatc
     3301 atccggatca tcttcgccgg gtcctcggcc ttgtcgagca ggtcgttgac gttcgcggcg
     3361 acgatgtcgc gggtgcggga gaagatgctc atgcggcgga actccttggg gccgcgatag
     3421 ggacgcgggg tgtggcgggg tgtcggccgg ggcggaatgg gggcaagttc cccccggccg
     3481 acgatgacca gttcaggggt gcggtcaggc atgggcctgg tccgcgccct caacctgggc
     3541 ataagcgatg ggagcggttt cgatggcgcg ggccgggccg accgcggcgc cgaccgaaac
     3601 ggcggtgagg atgagggcgc cgagcgcggc agcggcgacc tgctggaatt ttccgaaatc
     3661 gaacatcgtg ggtctcctga accttggcgt ctgtctcatc ccgccacccc gttggggcgg
     3721 cgatgcacct tgggtttcaa gagccgtgcc aaagtcgagc aatggcgttt ttccgcgggt
     3781 ttttggcccg ggcgccgctc agtggcggat tttccctgct atcggttggg agttttcacc
     3841 aactttttgg aggaagccgc cgcggaaagc tgggccccgg ctttcgccgg ggagcagggc
     3901 tggctcaggc ctttgcccgg cggtcgccga ggcggcggtc ggtgcctgga cggcggcgcg
     3961 gcgcggtggc gtcgaggatc ttggcgccgg ccatgaacac cgccccggtg caggcggcga
     4021 agaacaccca gccgagcggg ccgggataga tatcgagata atgatagccg cggctgaccg
     4081 cgccgacggc gatcgcgtag atcacggcga acgcctcgaa ccgggtcttg atgatgaaca
     4141 gccgcaggaa cttttggagc atgagcaacg aggagcaaga agcgggccag ccacgcaaaa
     4201 gctggcgaag cagggttaag gtttaacgac ttgtaaacca tggcgacgaa atcggccgat
     4261 ccggggcagc ggaggaggcg ccgtggcggg gacgcacggg ggcgagcgag gggccagcgg
     4321 gaaccagccg ccgccgtcgg ggcttaggtc cccgacggag ggccgagatg cgaagccatg
     4381 aggagatcgt gaccgaggtg gcggggcgga gcccggcctg cgccaaggtc ggcgtgttcg
     4441 acatcgacgg cattttccgc ggcaaatata tggccgtgga caagctcggc tcgacgctgg
     4501 ccaagggctt cggcttttgc gacgtcatct tcggctggga ttccaacgac cagctgctcg
     4561 acaatctcaa tttcaccggc tggcacagcg cctatcccga cgctgaggcg cggctgctgc
     4621 cggagacgtt gcgggcgatg ccgttcgagg acggcatgcc gctgatcctg ggcgagttca
     4681 gcggccgggc cgaggcggtc tgcccgcgcg gcaccttgcg ccgcatcctc gaccgcgccg
     4741 cgggcctcgg cttcaaggtc aaggcttcgg ccgagttcga attcttcgtg ttcgaggaga
     4801 cgccgcaatc ggtccgcgac aagggctatc gcaacctcaa gtcgatcacg cccggctatt
     4861 tcggctattc gatgctgcgc tccggcgtcc attcggattt ctaccgcgag ctgtggtcga
     4921 cctgcgatgc gatgcggatc gagctcgagg ggctccatac cgagaccggg ccgggtgtga
     4981 tcgaggcggc gatcaaggtc gacgatgcac tcgaggcggc ggacaaggcg gccctgttca
     5041 agaccatcgc caagatcctc gcccagcggc gcggctggat ggcgaccttc atggccaaat
     5101 ggtcgctcga ctggccgggc cagtcgggcc acctccacat gtcgctgacg gatgccgaga
     5161 caggcgagcc cattttctac gaagagggcg cgcggcacgg aatgtcggac cggctgcgct
     5221 ggttcgtcgg cgggcagcag gcgctgatgc ccgaattgct ggcgatgctg gcgccgacgg
     5281 tcaacgctta cacccggctc gtccccggct attgggcgcc gaccgcggcg acctggggct
     5341 atgagaaccg gacctgcgcg ctgcgcgtga tcggcgggtc ggcccactcg cagcgggtcg
     5401 agtgccggat cggcgcggcc gacatcaacc cctatgtcgc gctcgccgcg gcgatcggat
     5461 cgggcctgtg ggggatcgag cataagattg agccggacga gcccatcgtg ggcaatgcct
     5521 atgaggccga gatgccggtg gagcgctcgc tcccctcgac cttgtgggac gcggcccagc
     5581 ggctgcgcgg ctcggcggcg gcgcgcgacc tgttcggatc ggagttcgtc gaccattatg
     5641 cttccacccg cgaatgggag gagcgcgagg cgcgcaaggc ggtgaccgac tggcagctcg
     5701 cccgctattt cgagatcatc tgatggggtg cggccgttga gcgacgacct cgtctgcatc
     5761 tcgccggtcg acgggtgcga atatgcgcgg cgggcgctga cgccgccggc ggaggtggac
     5821 cgggcgctgg cgcgggcgcg ggcggcgcag cgcgaatggg cgcgggcgcc gctcgccgag
     5881 cgctgcgcgg tggcgctgcg cttccttgac gcgatggagc ggctcaatgc cgagatcgtg
     5941 ccggagctcg cctggcagat ggggcggccg gtgcgctacc agggcgaatt gcgctcgctg
     6001 gtcgaccgcg tgaagggcct ggtggcggtg gcggaagacg cgctggcgcc ggacgagccg
     6061 gagcccggcg gcggggcgcg ccgctatgtc acgcgggtgc cggccggcct ggtgctggtg
     6121 atcgcgccct ggaattatcc gttcctgacc gccgccaaca ccatcgtgcc cgctctggtc
     6181 gccggcaatg cggtggtgct gaaacatgcc agccagactt tgctcgcggg cgaacgcttc
     6241 gcccaggcgc tgcaactcgc ggggctgccc cagggcctgt tccacaatct cttcctcagc
     6301 cacgaggaga ccgcgcggct gctcggatcg ggtctggtcg atcacgccac cttcaccgga
     6361 tcggtcgagg gcgggcgggc catcgagcgg gcggcggcgg gatcgttcac cactttgacg
     6421 ctggagctgg gcggcaagga ccccgcttat gtgcgggcgg atgccgacat cgccggcgcc
     6481 gtcgaaagcc tggtcgacgg cagcttctac aatagcggcc aatcctgctg cggggtcgag
     6541 cgcatctatg tcgacgaggc ggtgtggcag ccgttcctcg acggtttcgt cgcggcgacg
     6601 cagcaatatc ggctcggcga tccgctcgat cgggagacca gcctcggccc gatgtcgagc
     6661 gccagactgg ccgggcaggt tcgcgggcag gtcgatgagg cggtcgcggc cggcgccgag
     6721 gcgcatatcg agcggcagct gttccgcgcc gacgcggccg gcacggccta tctgatgccg
     6781 caggtgctga ccggggtgga ccacgaaatg gcggtgatgc gcgaggagac gttcgggccg
     6841 gtggtcggga tcatgtccgt gaaaagcgac gacgaggcgg tgcggctgat gaacgattcc
     6901 cgctacggcc tcaccgcctc gatctggacc cgcgacgcgg cggcggccga gcggatcgcg
     6961 cccgaggtcc aagccggcac ggtgttcatg aaccgctgcg actatctcga ccccctgctc
     7021 gcctggaccg gcgtcaagga gagcgggcgc ggcgcctcgc tcggccgcta cggctatgag
     7081 ggcgtgacgc ggccgaaatc ctatcatctg aggagcggcg catgaccctg ccgagcgcca
     7141 actggaatta tccgacggcg atccgcttcg gcgcggggcg aatcgccgag cttgcggagc
     7201 tgtgccggga gctcggcatc gagcggccgc tgctggtgac cgatcccggc ctcgcggcct
     7261 tgccgatgat cgcggcggcg gtcgaggcga atggcgcggc gggcgtgccg accggcctgt
     7321 tctccgaagt gcgcggcaac ccggtcgggg gcaatgtcga ggcgggcgtc gtcgtttacg
     7381 cggccggcgg gcatgacggg gtgatcgcgt tcggcggcgg cagcgcgctc gacgccggca
     7441 aggcgatcgc cttcatgtcg ggccagagcc ggccgatctg ggacttcgag gacaaggccg
     7501 attggtggac gcgcgccgac ccagccggca tcgctcccgt gatcgcggtc ccgaccaccg
     7561 ccggcaccgg ctcggaagtc ggccgcgcca gcgtcatcct cgacgaggat gagggggtaa
     7621 agaagatcat cttccacccg aagatgatgc cggccgcggt gatcctcgat ccggagctca
     7681 ccgtcggact gccgccgcat atcaccgcgg cgacgggcat ggacgcgctg tcgcactgca
     7741 tcgaggccta ttgcgcgccc ggattccacc cgatggccga cggcatcgcg gcggaaggcg
     7801 tgcggctgat ccacgactgg ctgccgcggg cggtggcgga cgggaccgac atcgcggcgc
     7861 gcgggcatat gctgatcgcg gcttcgatgg gcgcgaccgc cttccagaag gggctcggcg
//
LOCUS       DASVRK010000299         6809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_605985, whole genome shotgun sequence.
ACCESSION   DASVRK010000299 DASVRK010000000
VERSION     DASVRK010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605985"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..674)
                     /locus_tag="VF589_12500"
     CDS             complement(213..674)
                     /locus_tag="VF589_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8258442.1"
                     /translation="MKTDYHKALLTECALPGALEAVGERWSFLILRGVFNGLHHFEEF
                     QSTLGIARNILSNRLARLVEHDILRREADPSDRRKVAYRLTDQGRALLPVLIALRQWG
                     ERWVSGMPSNPVLVDRSSRLPVAPMEVRATDGRPLSLAELEWLDRSEIAGA"
     gene            795..1100
                     /locus_tag="VF589_12505"
     CDS             795..1100
                     /locus_tag="VF589_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258443.1"
                     /translation="MTRASALALAPLFALAFASGPALAASHYRAEPARAPAEAKIVLR
                     DTIWNCTDQGCAAPKSNSRPAIVCSLLVKEVGQLRSFSVGGEPMPADQLEKCNARGG"
     gene            1149..3236
                     /locus_tag="VF589_12510"
     CDS             1149..3236
                     /locus_tag="VF589_12510"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX8258444.1"
                     /translation="MLRQYELLEKVRAYDPDADEALINRAYVFSMKAHGGQKRASGDP
                     YYSHPIEVAGILTDLHLDDETIVTAILHDTIEDTVATAEEVEKLFGKSVARLVDGVTK
                     LSKIEAQSESQRAAENLRKFLLAMSGDIRVLLVKLADRLHNMRTLHFIKDEEKRRRIA
                     RETMDIYAPLAERIGMYEFMTEMQTLAFRQLEPDAYASISKRLEQLHKGGGDLIDRIG
                     RGLKAHLEAHGIEAEVQGREKHPYSIWKKMAERHVSFEQLSDVMAFRAIVDNVEDCYR
                     TLGLIHQRWPMVPGRFKDFISTPKRNGYRSVHTSVIHDEQMRIEIQIRTREMHARAEH
                     GLAAHWAYKEGKAAATTQHPWISDLIEILEHAGSAEELLEHTRMAMYQDRIFAFTPKG
                     ELIQLPKGATPVDFAYAVHTDLGDQTVGAKVNGRVMPLRTPLDNGDQVEILVSKAQHP
                     QPAWLSFVVTGKAKAKIRRFVKSKEREETIALGRKIFDEIVQRLPTPLGWEAIAEALR
                     RLHLHDDDALMEAIALKRVDDVHVMEALMPGSALKEGVKPPPQRRAISIKGLTPGVAF
                     DLAECCHPIPGDRIVGLRREGTGIEVHTIGCDRLADGIDADWVDLAWGDESDGGAARL
                     CVVIKNEPGSLAVITGILGSHGANIVSLDQISRDGSFHTFHLDIEVHDVQHLMRILAA
                     LRATDVVSTAERL"
     gene            3241..4041
                     /locus_tag="VF589_12515"
     CDS             3241..4041
                     /locus_tag="VF589_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX8258445.1"
                     /translation="MTDTPASMSYAGYLTLDQLLTAQHPISDLHDEMLFVIIHQTKEL
                     WLKQVIHELKLSLGLVRADQLIPVHKSLSRVTRIQAVMTLSWDVLATMTPTDYTSFRS
                     VLGSSSGFQSGQFRQVEYLLGLKDLAHLRYQEEGSDAHGALAEALHRPSLWDEANAAL
                     ARAGLDLPAHVLERDWSQPYEPSPAVEAAWTEVYRDTARWWPLYQLAEKLVDIDDALA
                     TWRHKHIVTVERIIGLKRGTGGSAGVPYLQSTLGKRAFPELWSLRTSI"
     gene            complement(4015..4983)
                     /locus_tag="VF589_12520"
     CDS             complement(4015..4983)
                     /locus_tag="VF589_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8258446.1"
                     /translation="MLIASLAALLALQPAAPAEPAGAASFVRAEDHRVAEIGYRMGLA
                     GRPFCAEPYPLTGLLLQHLGEYAAADQKQAMTEFGLDRGPGVLAVVAGSPAARAGLAA
                     GDIMLAVNGAPLAPASGMPRPGRGDAARRAVEAVEGRIEAELRKGPARLTVARGGDEL
                     TVELSSVAGCPARVRLARSGQMNAFANRGYAIVTTALLGFIRSDDELAVVLGHELGHV
                     ILKHPEQLEAEGVPGRGFLRAFGKNGSRVRATEIAADGLGLRLASAAGYDAAAAIPYW
                     RRYYASFDSLPRLFRTHPGLRERERLAQETLAELRAGGSDAGAERP"
     gene            complement(5167..5367)
                     /locus_tag="VF589_12525"
     CDS             complement(5167..5367)
                     /locus_tag="VF589_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258447.1"
                     /translation="MAATPALAAGAAARLAPAPERIFGSAQGEDDDDGSGVIIAVLAG
                     LAVLGGIWAAVGGDDDEAVSPD"
     gene            5834..>6809
                     /locus_tag="VF589_12530"
     CDS             5834..>6809
                     /locus_tag="VF589_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8258448.1"
                     /translation="MHQSLIKAAATRAAIDEKIVIVGLGYVGLPLAVALARGAAVVGV
                     DIDRSRIEEIQAGVDRTNEVSPEALRASSLEVTSDPAICAGADIFIVTVPTPVDGSSR
                     PDLRPLLGATETVAGLLDPKRRPIIVYESTVYPGVTEDICGPLIEKVSGLKRGTDFFL
                     GYSPERINPGDREHTVDRIVKVIAGENDEVTERLAAIYGAVTSAGVFRAASIRAAEAA
                     KVIENAQRDINIAFMNEITRIFSKLDISIWDVLDAAGTKWNFLKFQPGLVGGHCIGVD
                     PYYLSYRAQQLGKEPVVILAGRSINDGMGSWMADQLHARRGKAGTALVM"
BASE COUNT         1206 a         2393 c         2167 g         1043 t
ORIGIN      
        1 gcagcccgga attgttcgat agtctgccgc tggcggtcaa gccgggcggc tgaaagtggt
       61 ggtgaaggtg gtgcttgaaa gtggccaccg tcatccagcg aaagccggga tctcacttct
      121 tgcggtgggc taagggggta gggagattcc agctttcgct ggaatgacgg gccaggttag
      181 gtctcagccg taccaccgcc acgccgcccc ggtcaggcgc cggcgatctc gctgcggtcg
      241 agccattcga gctcggccag cgacagcggg cggccgtcgg tggcgcggac ttccatcggc
      301 gccaccggca gccggctcga gcggtcgacc agcaccgggt tgctcggcat gcccgatacc
      361 cagcgctcgc cccattgccg tagcgcgatc agcaccggca gcagcgcccg gccctggtcg
      421 gtcagccggt aggcgacctt gcggcggtcg gaaggatcgg cctcgcgccg caagatgtcg
      481 tgctcgacca ggcgggccag ccggttcgac aagatgttgc gggcgatgcc gagcgtcgac
      541 tggaactcct cgaaatgatg aaggccgttg aacacgccgc gcaggatcag gaacgaccag
      601 cgttcgccga ccgcctcgag cgcgccgggc agggcgcact cggtgagcag cgccttgtga
      661 tagtcggtct tcatagatcg gcctcaatct acgccttttt tcggctgtaa caactgacac
      721 gcaaatcccg ttgcgaaacg aaaccgacct cggtataggt tgcaaatagc aacttagcaa
      781 agggagcttt tccaatgacc cgcgcctccg cactcgcgct cgcccccctg ttcgccctcg
      841 ccttcgcgtc cgggccggcg ctggcggcat cccactaccg cgccgaaccc gcccgcgcgc
      901 cggccgaggc caagatcgtg cttcgcgaca cgatctggaa ttgcaccgat cagggctgcg
      961 ccgcgccgaa gagcaacagc cggccggcga tcgtgtgctc attgctcgtc aaggaagtcg
     1021 gccagcttcg cagcttcagc gtcggcggcg agccgatgcc cgccgatcag ctcgaaaagt
     1081 gcaacgcccg cggcggctaa agcctttttg cattgcaaca acccggcacc ggaccccatg
     1141 ttggtccggt gctgaggcaa tatgagctgc tcgagaaggt ccgggcctat gatcccgatg
     1201 ccgacgaggc gctgatcaac cgcgcctacg tcttctcgat gaaggcgcat ggcggccaga
     1261 agcgcgcctc gggcgacccc tattacagcc acccgatcga ggtggccggc atcctcaccg
     1321 acctccatct cgacgacgag accatcgtca ccgccatcct ccacgacacg atcgaggata
     1381 ccgtcgccac cgcggaggaa gtcgagaagc tgttcggcaa gagcgtggcg cgcttggtcg
     1441 acggtgtcac caagctctcc aagatcgagg cgcagagcga aagccagcgc gccgccgaga
     1501 atttgcgcaa gttcctgctc gccatgtcgg gcgacatccg cgtgctcctg gtgaagctcg
     1561 ccgaccggct gcacaacatg cgcacgctcc acttcatcaa ggatgaggag aagcgccggc
     1621 gcatcgcccg cgagacgatg gacatctacg cgccgctcgc cgagcggatc ggcatgtacg
     1681 agttcatgac cgagatgcag acgctggctt tccggcagct ggagccggac gcctatgcct
     1741 cgatctcgaa gcggctcgag cagctccaca agggcggcgg cgacctgatc gaccggatcg
     1801 gccgcggcct caaggcgcat ctcgaggcgc acggcatcga agccgaggtg cagggccgcg
     1861 agaagcaccc ttattcgatc tggaagaaga tggccgagcg ccatgtcagc ttcgagcaat
     1921 tgtccgacgt catggcgttc cgcgcgatcg tcgacaatgt cgaggattgc taccgcactt
     1981 tgggcctcat ccaccagcgc tggccgatgg tgcccggccg gttcaaggac ttcatctcga
     2041 cccccaagcg caacggctat cggtcggtcc atacctcggt catccacgac gagcagatgc
     2101 ggatcgaaat ccagatccgc acccgcgaga tgcacgcccg cgccgagcac gggcttgccg
     2161 cccactgggc ctataaggaa ggcaaggcgg ccgccaccac ccagcacccc tggatcagcg
     2221 acctcatcga gatcctcgaa catgccggca gcgccgagga gctgctcgaa cataccagaa
     2281 tggcgatgta ccaggaccgg atcttcgcct tcacccccaa gggcgagctg atccagcttc
     2341 cgaagggcgc gacgccggtc gatttcgctt atgccgtcca caccgatctc ggcgaccaga
     2401 cggtcggcgc caaggtcaac ggccgcgtga tgccgctgcg caccccgctc gacaatggtg
     2461 accaggtcga gatcctcgtc tccaaggcgc agcacccgca gccggcctgg ctgagcttcg
     2521 tcgtcaccgg caaggccaag gccaagatcc gccgcttcgt caaatccaag gagcgcgagg
     2581 agacgatcgc gctcggccgc aagattttcg acgagatcgt ccagcgcctg cccaccccgc
     2641 tcggctggga agccatcgcc gaagcgctgc gccgcctcca cctccacgat gatgatgccc
     2701 tgatggaggc gatcgcgctg aagcgcgtcg acgacgtcca tgtgatggag gcgctgatgc
     2761 ccggctcggc gctgaaggaa ggcgtgaagc cgccgccgca gcgccgcgcc atctcgatca
     2821 agggcctgac ccccggcgtc gccttcgatc tcgccgaatg ctgccacccg atccccggcg
     2881 accgcatcgt tgggcttcgc cgcgaaggca ccggcatcga agtccatacg atcggctgcg
     2941 accggctcgc cgacggcatc gacgccgatt gggtcgatct cgcctggggc gacgaatcgg
     3001 acggcggggc ggcgcggctg tgcgtcgtca tcaagaacga gcccggctcg ctggccgtga
     3061 tcaccggcat cctcggcagc cacggcgcca atatcgtcag cctcgaccag atcagccgcg
     3121 acggcagctt tcacaccttc cacctcgata tcgaggtcca tgacgtccag cacctgatgc
     3181 gcatcctcgc cgcgctccgg gccaccgacg tcgtctccac cgcggagcgg ctatagcagc
     3241 atgaccgaca ccccagcctc gatgagctat gccggctatc tgacgctcga ccagctgctg
     3301 acggcccagc acccgatttc ggaccttcac gacgagatgc tgttcgtgat catccaccag
     3361 accaaggagc tttggctcaa gcaggtgatc cacgaattga agctgtcgct gggcctcgtc
     3421 cgcgccgacc agctgatccc ggtccacaag agcctgtcgc gcgtcacccg catccaggcg
     3481 gtgatgacgc tgagctggga cgtgctcgcg acgatgacgc cgaccgatta taccagcttc
     3541 cgcagtgtgc tcggctcgag ctcgggcttc cagtcgggcc agttccggca ggtcgaatat
     3601 ctgctcggcc tcaaggacct cgcccacctt cgctatcagg aggaaggctc cgacgcccac
     3661 ggcgcgctcg ccgaggcgct gcaccggccg agcctgtggg atgaagccaa tgccgcgctc
     3721 gcgcgcgccg ggctcgacct gcccgcccac gtgctggagc gggactggag ccagccttac
     3781 gagcccagcc ccgcggtcga ggcggcctgg accgaagtct atcgcgacac cgcgcgctgg
     3841 tggccgctct accagctggc cgagaaattg gtcgacatcg acgacgcgct cgccacctgg
     3901 cggcacaagc atatcgtcac cgtggagcgg atcatcggcc tgaagcgcgg caccggcggc
     3961 agtgccggcg ttccctatct ccaatcgacg ctcggcaagc gcgcctttcc cgaattatgg
     4021 tcgctccgca ccagcatctg agccgccggc ccgcaattcc gccaacgtct cttgcgccag
     4081 ccgctcgcgt tcgcgcaggc ccggatgggt gcggaatagc cgcggcaggc tgtcgaacga
     4141 ggcataataa cgccgccaat aagggatcgc cgccgcggcg tcgtagccgg ccgccgaggc
     4201 cagcctgagc cccagcccgt cggcggcgat ctcggtcgcc cgcacccgcg atccgttctt
     4261 gccgaaggcg cgcaggaagc cgcggcccgg gacgccctcc gcctcgagct gctccggatg
     4321 cttcaggata acatggccga gctcgtggcc gagcacgacc gccagctcgt cgtcgctgcg
     4381 gatgaagccg agcaaggcgg tggtgacgat cgcatagccg cgattggcga aggcgttcat
     4441 ctgccccgac cgcgcgagcc ggacccgggc cgggcagccg gcgacggagc tgagctccac
     4501 cgtcagctcg tcgccgccgc gcgccacggt caggcgcgcc ggccccttgc gcagttccgc
     4561 ctcgatccgc ccctcgaccg cctcgacagc gcgccgcgcc gcgtcgcccc ggccgggtcg
     4621 cggcatcccg gacgccggtg ccagcggcgc gccgttcacc gccagcatga tatcgcccgc
     4681 ggcgaggccg gcccgcgcgg ccgggctgcc cgccaccacc gccagtacgc ccggcccgcg
     4741 gtcgagcccg aactcggtca tcgcctgctt ctggtcggcc gcggcatatt cgcccaaatg
     4801 ttggagcagg agcccggtca gcgggtaggg ctccgcacag aaaggccgcc cggccaggcc
     4861 catgcggtag ccgatctcgg ccacgcgatg gtcttccgcg cgcacgaagg aagccgcgcc
     4921 ggccggctcg gccggggcgg cgggctgcag cgccagcagt gcggcgaggg aggcgatcag
     4981 cacagccggc atccacgatg gcgccgcaac ggcccgagct tcaccgcaac gggcgggccg
     5041 gcggtgggtc ggacaaacag ggggcggggc agggcgctgg cctccagacg cgaacggggc
     5101 ctacaaacgc aaatggccgg gtgaagcgat cctcacccgg ccaatggctt gttcagtgac
     5161 gaacgatcag tccgggctca ccgcttcgtc gtcgtcgccg ccgacggcgg cccagatgcc
     5221 gccgagcacg gcgaggccgg ccagcacggc gatgatcacg ccgctgccgt catcgtcgtc
     5281 ttcaccctgc gccgagccga aaatcctttc gggcgccgga gccaggcggg cggcagcgcc
     5341 ggcggcgagc gccggcgtcg cagccaaagc aacagccgca gtcgcagcga taagtccctt
     5401 gaaaaccacg tcagcttctc ccttcgtact caaaacttat tatcacgatt tcccgttctg
     5461 attatccgag gcgtgtttga gtgtccaccc cgaaatgcgc cgcggaaacc ggcgctttcc
     5521 agcccattat tgcctgtgat ttgccaagtc gttaacccaa atcatcgcag cggcgcctgc
     5581 ccaggcttcg ctgaagtggc acattttcaa agcctacaca atggcttagc aatatgtgcc
     5641 tggcttgctg ggcacggttg ccgcagttgc gggggcgaag cctccgcatc gcttgccgcc
     5701 gcgcggagcc aggccctcgc gcggcaaaag aatcgccccc ctattgcatt gcagcaacag
     5761 ctgcgtgtgc tcagccacac ctcggttgac cgggaggtaa ggtttccctg ctagcggcgg
     5821 ggctggagaa accatgcacc agtcccttat caaagccgcc gcgacgcgcg cggccataga
     5881 cgagaagatc gtcatcgtcg gcctcggtta tgtcggcctg ccgctggcgg tcgcgcttgc
     5941 ccgcggcgcc gccgtggtcg gcgtcgatat cgaccgctcg cgcatcgaag agatccaggc
     6001 gggcgtcgac cgcaccaacg aggtcagccc cgaagccttg cgggcgagca gcctcgaagt
     6061 gaccagcgac ccggcaatct gcgccggcgc cgacatcttc atcgtcaccg tgccgacccc
     6121 ggtcgacggc agcagccgcc cggacctgcg gccgctgctc ggcgccaccg agaccgtcgc
     6181 cgggctgctc gatccgaagc ggcggccgat catcgtctat gaaagcaccg tctatcccgg
     6241 cgtcaccgag gacatttgcg ggccgctgat cgagaaggtg tcgggcctga agcgcggcac
     6301 cgacttcttc ctcggctatt cgcccgagcg catcaacccg ggcgaccgcg agcatacggt
     6361 ggaccgcatc gtgaaggtaa tcgccggcga gaacgacgag gtcacggagc gcctcgccgc
     6421 catctacggc gcggtgacca gcgcgggcgt gttccgcgcg gcctcaatcc gcgccgccga
     6481 ggcggccaag gtgatcgaaa acgcccagcg cgacatcaac atcgccttca tgaacgagat
     6541 cacccggatc ttctccaagc tcgacatttc gatctgggat gtgctggacg cggccgggac
     6601 caagtggaac ttcctcaagt tccagccggg cctggtcggc ggacactgca tcggagtgga
     6661 tccctattat ctcagctatc gggcccagca gctcgggaaa gagccggtcg tcatccttgc
     6721 cggccgctcg atcaacgacg gcatgggcag ctggatggcg gatcagctgc atgcgcgccg
     6781 cggcaaagcc ggcacggccc tggtcatgg
//
LOCUS       DASVRK010000300         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_554244, whole genome shotgun sequence.
ACCESSION   DASVRK010000300 DASVRK010000000
VERSION     DASVRK010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554244"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VF589_12535"
     CDS             complement(<1..1568)
                     /locus_tag="VF589_12535"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX8258449.1"
                     /translation="MTSRESMPYDVVIVGAGPAGLAAGIRLKQLAAEAGQELSVCILE
                     KGSEVGAHILSGAVIDPRGLDELLPNWKELDSPLTVPVVENRHWVLTHAKKYELPHIM
                     MPPFMSNKGTYTASLGNVCRWLAGQAEALGVEIFPGFAAAEILFHDNGNVKGVATGDM
                     GVARDGTHKPDYQPGMELHGKYTFFAEGARGHLTKELKRIFKLDEGSEPQVYGIGLKE
                     LWDVDPSKHVPGRVIHTQGWPLEDAWGGGFIYHQDAGQVALGFVVALNYKNPYLSPFE
                     EFQRWKQHPAVREMLEGGRRVSYGARAINEGGWQAVPKLAFPGGALIGCSAGFVNVPR
                     IKGSHTAIKSGMLAAEAAFAAIGAGREADELTAYPEALGKSWIADELKMVRNVEPMVA
                     RYGGTLGTLLAGTDMWMRYLKIGLPFTMKHHPDNETLRRKDASRPIDYPKPDGVISFD
                     RLSSVFLSNTNHEEDQPVHLTLKDASIPVAVNLADYDGPEQRYCPAGVYEYVEEAGRR
                     RLQINAQNCVHCKT"
     gene            1700..2401
                     /locus_tag="VF589_12540"
     CDS             1700..2401
                     /locus_tag="VF589_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX8258450.1"
                     /translation="MSPADAASVLQWWTDAGVDVLVDEAPRDWLRPKAKAAPAAPQAK
                     VEAPDALPDQLELFHGFLRDSGTLPFATPVAPRVCPSGDPASGLMMMIDMPSDEDCGG
                     GTLLSGESGRLFDRMLAAIGRDRDSIYLASLSCLRSPAGFSSDSAAKCATLARHQIGL
                     AAPKALVLFGDTCAKALLGLGMAQARGRWHEVATHAGPVRTIVTLPPDLLLGRPGLKA
                     HAWADLQMLMQGFDR"
     gene            2398..>3124
                     /locus_tag="VF589_12545"
     CDS             2398..>3124
                     /locus_tag="VF589_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8258451.1"
                     /translation="MNMLRALLLSAAALPVTPLLAADASPTLARPLQNPAKPAVLSAA
                     ERSRYREIFAAIRAGDWSGAAARIDAGPRGPLDAAARAELYLAKGSPTVSLERLTALI
                     AEAPDLPQAAQLARLAQTRGAPPETLPGIAPAQRLVWAGSQPRRGRAGSVKGDAAAAE
                     LEALVQPLIVENQPSEAEAQYNSRAYGLTPEARTEYQQRIAWSYYLIGNDGAARRLAD
                     MARSGTGEWALHAAWVSGLASWRM"
BASE COUNT          458 a         1051 c         1098 g          517 t
ORIGIN      
        1 caggtcttgc agtggacgca attctgggcg ttgatctgaa gccgccgcct tccggcttcc
       61 tcgacatatt cgtaaacgcc ggccgggcag taacgctgct ccgggccgtc ataatcggcg
      121 aggttgaccg cgaccgggat cgacgcgtcc ttgagggtga ggtggaccgg ctggtcctcc
      181 tcatgattgg tgttggacag gaacaccgag gacaggcggt cgaagctgat cacgccgtcg
      241 ggcttgggat agtcgatcgg ccgggacgcg tccttgcggc gcagcgtctc gttgtcggga
      301 tggtgcttca tggtgaaggg cagcccgatc ttgagatatc gcatccacat gtcggtgccg
      361 gcgagcaagg tgcccaaggt gccgccgtag cgggcgacca tcggctcgac gttgcggacc
      421 atcttgagct cgtcggcgat ccagctcttg cccaaagctt cgggataagc ggtgagctcg
      481 tcggcctcgc gcccggcgcc gatcgcggcg aaggcggctt cggcggcgag catgcccgac
      541 ttgatcgcgg tgtggctgcc cttgatcctc ggcacgttga cgaagcccgc cgagcagcca
      601 atcagcgcgc cgccggggaa agcgagcttc ggcaccgcct gccagccgcc ctcgttgatc
      661 gcgcgcgcgc cgtagctgac gcggcggccg ccctccagca tctcgcgcac cgccggatgc
      721 tgcttccagc gctggaattc ctcgaacggc gagagatagg ggttcttgta attgagcgcc
      781 acgacgaagc ccagggcgac ctgacccgcg tcctgatgat agatgaagcc gccgccccag
      841 gcgtcctcga gcggccagcc ctgggtgtgg atgacgcggc cgggcacatg cttggacggg
      901 tcgacatccc aaagctcttt gaggccgatc ccatagactt gcggctcgct tccctcatcg
      961 agcttgaaga tgcgcttcag ctccttcgtg aggtggccgc gcgcgccctc ggcgaagaaa
     1021 gtgtatttgc cgtggagctc catgccgggc tggtagtcgg gcttgtgggt gccgtcgcgg
     1081 gccacgccca tgtcgccggt cgcgacgccc ttcacattgc cattgtcgtg gaacaggatc
     1141 tcggccgccg cgaagccggg gaaaatctcg acccccagcg cctccgcctg ccccgcgagc
     1201 cagcggcaga cattgccgag cgaggccgtg taggtgccct tgttgctcat gaagggcggc
     1261 atcatgatgt ggggcaattc gtatttcttg gcgtgggtca gcacccagtg gcggttctcg
     1321 accaccggca cggtgagcgg actgtcgagc tccttccaat tggggagcaa ttcgtcgagt
     1381 ccgcgcggat cgatcaccgc gcccgacagg atgtgggcgc cgacttcgga tcctttttcg
     1441 aggatgcaga cggacagctc ctgccccgcc tcggccgcga gctgcttgag gcggatgccg
     1501 gccgcgagcc cggcggggcc ggcgccgacg atcaccacgt cataaggcat ggattcgcgg
     1561 ctggtcatgc aggtctccgt tacggggccg tcccttggtc gcaaacggcc aacgtctggc
     1621 agataagcgc ccgatcggag gttgaccagc ccgcgggagc ctgactctat gggcggctgt
     1681 ggagcgggga agcgatccat tgtcccctgc ggacgcggcg agcgtcctgc agtggtggac
     1741 cgatgcgggg gtcgacgttc tggtcgacga agccccgcgc gactggctgc gcccgaaggc
     1801 gaaggcggcg ccggccgctc cgcaggccaa agtcgaggcg cccgatgcgc ttcccgacca
     1861 gctcgagctg ttccacggtt tcctgcggga cagcggcacc cttccgttcg ccacgcccgt
     1921 ggcgccgagg gtctgcccgt cgggcgaccc agcgtcgggg ctgatgatga tgatcgacat
     1981 gccgtcggac gaggattgcg gcggcggcac cttgctgtcg ggcgagagcg ggcggctgtt
     2041 cgaccggatg ctggccgcga tcgggcggga ccgggactcg atctatctcg cctcgctgtc
     2101 ctgcctgcgc tcgccagccg ggttcagcag cgactcggcc gccaaatgcg ccacgctggc
     2161 gcgccatcag atcgggctgg cggctcccaa ggcgttggtg ctgttcggcg acacctgcgc
     2221 caaggcgttg ctcggcctcg gcatggcgca ggcgcgcggg cgctggcacg aagtggccac
     2281 ccatgcgggg ccggttagga cgatcgtaac cctcccgccc gatcttctgc tcgggcggcc
     2341 tggcctcaag gcgcatgcct gggccgacct gcaaatgttg atgcaaggat tcgaccgatg
     2401 aacatgcttc gcgccctgtt gctgtccgcc gccgcccttc ccgtcacgcc gctgctggcc
     2461 gccgacgcca gcccgacgct ggcgcggccg ctccagaacc cggccaagcc ggccgtgctg
     2521 agcgccgccg aacgcagccg ctatcgcgag attttcgccg cgatccgggc cggcgactgg
     2581 agcggagccg cggcccgaat cgatgccggt ccgcgcgggc cgctcgacgc cgccgcgcgc
     2641 gccgagcttt atctggccaa gggttcgccg accgtgtcgc tcgagcggct gacggctttg
     2701 atcgccgaag cgccggatct gccgcaggcg gcgcagctgg ctcggctggc gcagacgcgg
     2761 ggcgccccgc cggagacgct gcccggcatc gcgcccgccc agcggctggt ctgggccggc
     2821 tcccagccgc gccggggccg ggccggcagc gtcaagggcg acgccgccgc ggccgagctt
     2881 gaggcgctgg tccagccgct gatcgtcgag aaccagcctt ccgaagccga ggcgcaatat
     2941 aacagccgcg cttacgggct gacgccggaa gctcgcactg aatatcagca acgcatcgca
     3001 tggtcatact atctgatcgg caatgacggc gcggcgcggc ggctcgccga catggcgcgg
     3061 agcggcaccg gcgagtgggc gctgcacgcg gcgtgggtgt ccggcctcgc ctcgtggcgg
     3121 atgc
//
LOCUS       DASVRK010000301         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_607031, whole genome shotgun sequence.
ACCESSION   DASVRK010000301 DASVRK010000000
VERSION     DASVRK010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607031"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VF589_12550"
     CDS             complement(<1..229)
                     /locus_tag="VF589_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8258452.1"
                     /translation="MNEPALTLSALAKTLPGGRKLFDGVDLALAPGEHVAVRGESGVG
                     KSTLLNIIAGLDEADAGEVAIAGQPLGALDEP"
     gene            345..767
                     /gene="ndk"
                     /locus_tag="VF589_12555"
     CDS             345..767
                     /gene="ndk"
                     /locus_tag="VF589_12555"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX8258453.1"
                     /translation="MATERTFSIIKPDATRRNLTGAVTKMLEDAGLRVVASKRIWMTR
                     DQAEGFYAVHRERPFFNDLVEFMISGPVVVQVLEGENAVARNREVMGATNPANADEGT
                     IRKAHAESIEANSVHGSDSAENARIEIDFFFDPQEIVG"
     gene            920..>3614
                     /locus_tag="VF589_12560"
     CDS             920..>3614
                     /locus_tag="VF589_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258454.1"
                     /translation="MANIDLKNNDYDGQTIVAVQYGSLSRFVGPAGDFDGDGFDDLLL
                     RSYYRHDGVKQEIEVWLGGPAPLERGFVFSGDPADDDNFADILSAIGDINGDGFDDII
                     GGDYRAGQITVYLGRLSGSEGSSFDIVGALPYRLDFAPAGDINADGLDDFIIGQPSAR
                     KAHVIFGTAEGHGQMDLSNLPAGQGFAIVGAETDRGTGHTVASAGDINDDGFDDIMVG
                     VYRDYASTADAPQAYVVFGKAGGFGTIDLGGLDPADGFAIIGKPEHASGSHLAAAGDV
                     NGDGIDDFMVGGPNRAFIIYGKSEGLGTVDLTALAPADGYRIAFNGDLRALTSAGDIN
                     GDGLDDIILAVNITPIGRASFVIYGQTGQSNTVELPNLDPERGFSLRGDNDYNAAGVG
                     DLNGDGFGDTAVTGHRDVHVIYGGARFNPVAFTGTDADEVAEGGIRGDELQGGGGNDI
                     LIGREGHDLIRGGDGDDVLSGGGDDDLIDGGAGSDTADYSLSLGRIHVDLTSGLQNGG
                     GAAGFDTLVSIENVIGSSAGSIIIGNGESNRLTGGNAVDRIQGGGGGDLLDGGGSDYD
                     MVDYSWAGIGVRVALDEAGPQDTNAGIDTLTGFENLTGSDFNDILGGDDGHNRLDGGK
                     GDDRLRGNAGYDTLVGGEGSDTAIYFGSAAGVTVSLNQNEQQTGGAGNDYLYEIENLV
                     GTSHADRLIGNAAVNFLDGGEGDDALDSGGGGGRMIGRGGDDRYTVRHVDDLVVEAAG
                     AGTELVISRIESYALGANVENLILEGLAGSGIGNGLDNYIRGSNPDDLGSTGDNHLEG
                     GGGDDLINGRGGNDTIDGGSGDDTLTGLGGTDTMSGGSGNDLFHVSDDADAVVEAEGG
                     GIDTVSSRIDYRLGANVENLILRSGISGIGNELANSIIGNNG"
BASE COUNT          670 a         1184 c         1206 g          554 t
ORIGIN      
        1 ccggctcgtc gagcgcgccg agcggctggc cggcgatcgc gacctcgccc gcatcggctt
       61 catcgagccc ggcgatgatg ttgagcaggg tcgacttgcc gacgccggat tcgccgcgga
      121 cggcgacatg ctcgcccggc gcgagcgcga gatcgacgcc gtcgaacagc ttgcggccgc
      181 cgggaagggt cttggccaaa gccgacaatg tcagggcggg ttcgttcatg gagcggcgat
      241 aggggcaagc ggctccggga taaagcggcg ctgcttgcgg tcggccgcgc cgccgcctag
      301 agagcgcgcc aatccacccc ccataaaacg gagctttgcc ggccatggcg accgaacgca
      361 ccttctcgat catcaagccc gacgcgaccc ggcgcaatct caccggcgcc gtcaccaaga
      421 tgctcgagga tgccggcctg cgcgtcgtcg cctccaagcg gatctggatg acccgcgacc
      481 aggccgaggg cttctacgcc gtccaccgcg agcgcccctt cttcaacgac ctcgtcgagt
      541 tcatgatctc ggggccggtc gtcgtccagg tgctcgaggg cgagaatgcg gtcgcgcgca
      601 accgcgaggt gatgggcgcc accaaccccg ccaatgccga cgaaggcacg atccgcaagg
      661 cccatgcgga gtcgatcgaa gccaattcgg tgcacggctc cgacagcgcg gagaatgcgc
      721 ggatcgagat cgacttcttc ttcgaccccc aggaaatcgt cggctgacgc cgagcccctg
      781 ccccccgggg gcagggcgag ccgggctgat ctaaaccggc tcctggagcg agaaagcggg
      841 tcggccccgc cgccgattcc ctcatatctt tgccccgcca aaactgttgt gggccagtaa
      901 cttgtggtgg ggactgcaca tggccaacat cgatcttaag aataacgatt atgacggcca
      961 gaccatcgtg gccgtccaat atggcagtct gagccgcttc gtcggcccgg ccggcgactt
     1021 cgacggcgac ggtttcgacg acttgctgct gagatcctat taccgccacg acggcgtcaa
     1081 acaggaaatc gaggtctggc tcggcgggcc cgcccccctc gagcgaggct tcgtctttag
     1141 cggcgatccc gccgacgacg acaatttcgc ggacatcctg tccgcgatcg gcgacatcaa
     1201 cggcgacggc ttcgacgaca ttatcggcgg cgactatcgc gccggccaga tcaccgtcta
     1261 tctcggccgg ctatcgggca gcgaaggcag cagcttcgat atcgttggcg cgctgcccta
     1321 ccggttggat ttcgctccag cgggggacat caacgccgac gggttggacg acttcatcat
     1381 cggccagcca tccgcccgca aggcccacgt catcttcggg acggccgaag gccacggcca
     1441 gatggatttg tcgaaccttc cggcgggtca gggcttcgcg atcgtcggcg ccgagaccga
     1501 ccgggggacg ggacacaccg tcgcgtcggc gggagacatc aatgacgacg gcttcgacga
     1561 catcatggtc ggagtctacc gcgactacgc gagtaccgcc gatgcgccgc aggcttacgt
     1621 ggtcttcggc aaggcgggtg ggttcggcac gatcgatctg ggtgggctcg accccgccga
     1681 cggcttcgcc atcatcggca aaccggagca tgcttccggc agccacctgg ccgcggccgg
     1741 cgacgtcaac ggcgacggga tcgacgactt catggtcggc ggacccaatc gcgccttcat
     1801 catttacggc aaaagcgagg gtctcggcac ggtcgatctg accgctttgg ctcccgccga
     1861 cggctaccgc atcgccttca acggcgacct tcgagcgttg acatccgcgg gcgacatcaa
     1921 cggcgacggg ctcgacgaca tcatccttgc cgtcaacatc accccaatcg gccgcgcctc
     1981 ctttgtgata tacgggcaga cgggacaatc caacacggtc gagctgccga acctggaccc
     2041 cgagcggggt ttctcgctcc gcggcgacaa tgactataac gcagccgggg tcggggatct
     2101 caacggcgac ggcttcgggg atactgccgt taccgggcac cgcgacgtcc acgtcatcta
     2161 cggcggcgcc cgtttcaatc cggtcgcctt caccggaacc gacgccgacg aggtggccga
     2221 gggcggaatc cgcggcgatg agctgcaggg cggcggcggc aacgacatac tcatcggccg
     2281 cgagggccac gacctcatcc gcggcggcga tggcgacgat gtgctgtcgg gaggcggcga
     2341 cgacgacctg atcgacggcg gcgccggctc cgacacggcc gattacagcc tctctctggg
     2401 caggatccac gtcgatctga cgtcggggct tcagaacggg ggaggcgcgg ccggattcga
     2461 caccctggtt tcgatcgaga atgtgatcgg ttcgtcggct ggaagcatca taatcggcaa
     2521 tggcgagagc aaccggctga ccggcggcaa tgcggtagac aggattcagg gcggcggcgg
     2581 cggcgacctg ctcgacggcg gcggcagcga ctatgacatg gttgactaca gctgggcggg
     2641 catcggcgtg cgcgtggcgc tggacgaggc ggggccgcag gatacgaatg ccggcatcga
     2701 tacgcttacc gggttcgaga atttgaccgg ttcggatttc aacgacattc tcggcggcga
     2761 cgacggccat aaccggctcg acggcggcaa aggcgacgat cgtctgcgcg gcaacgccgg
     2821 ctacgatacc ctggtcggcg gcgaggggag cgacaccgcg atctatttcg gctcggcggc
     2881 gggcgtcacc gtcagcctga atcaaaacga gcagcaaacc gggggcgccg ggaacgacta
     2941 tctgtacgag atcgagaatc tggtcgggac atctcatgcc gaccgcctga tcgggaatgc
     3001 cgccgtcaat ttcctcgacg gcggggaggg cgacgacgcc ctcgacagcg gcggcggcgg
     3061 cggccgcatg atcggccgtg gcggcgatga tcgctacacg gtcaggcatg tcgacgacct
     3121 cgtcgtcgag gcagccggag ccggaaccga gctcgtcatc tcgcggatcg aaagctacgc
     3181 gcttggggcg aacgtcgaga acctcatcct ggaagggctc gccgggagcg gcatcggcaa
     3241 cggcctcgac aattacatcc gcggcagcaa cccggacgat ctcggcagca ccggggacaa
     3301 tcatctcgag ggcggcggcg gcgacgatct catcaacggc cgcgggggca acgacacgat
     3361 cgacggcggc tccggcgacg acaccctgac gggcctcggc ggcaccgaca cgatgtcggg
     3421 gggctcgggc aacgatctct tccatgtgtc cgacgatgcc gacgcggtcg tcgaagcgga
     3481 gggcggcggc atcgacacgg tcagcagccg catcgattac cggcttggcg ccaatgtcga
     3541 gaatctcatc ctcagaagcg gcatcagcgg catcggcaac gaactcgcca acagcataat
     3601 cggcaataac ggcc
//
LOCUS       DASVRK010000302        18626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_555066, whole genome shotgun sequence.
ACCESSION   DASVRK010000302 DASVRK010000000
VERSION     DASVRK010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 18626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18626
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555066"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1175
                     /locus_tag="VF589_12565"
     CDS             153..1175
                     /locus_tag="VF589_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX8258455.1"
                     /translation="MNEMTKLTAKDFATDQEVRWCPGCGDYAVLKAVQRTMPELGVRP
                     ENVVFVSGIGCSSRFPYYMETYGFHTIHGRAPTFATGIKLANPELDVWIITGDGDALS
                     IGGNHTMHVLRRNLDCQILLFNNEIYGLTKGQYSPTSRVGTRSPSTPFGSVDRPASPC
                     AFALGSGARFVARAIDVNKNLPDVLKAAHAHKGAAFIEIFQNCIVYNDDVFAPFTAKD
                     NAGNQLWLKAGEPMLFAGGAKGITLDREALTLRVVEVADGDWQAAGVIVHEPANRSVA
                     HMLVEMPFGAFPMALGVIYDDPRPTFESAVVAQNAEASAGKVPNLQALVGKGQSWEVT
                     KEPHVI"
     gene            1172..1669
                     /locus_tag="VF589_12570"
     CDS             1172..1669
                     /locus_tag="VF589_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258456.1"
                     /translation="MMGLAAVLLIVGQGPAFKVVETPPPPDGQTPPPIVKGSPDGAIM
                     RVPRLDPAVALRSRGFSEGAIRTIMAAARTRMAARPAQMHMIERLKQELAVERSSDSP
                     SADRIEALLRRAAAASSADLSERVGRTLRLLRALPPADLKLYLRAFGPDEPEFLALSV
                     IPTVR"
     gene            1683..3062
                     /locus_tag="VF589_12575"
     CDS             1683..3062
                     /locus_tag="VF589_12575"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX8258457.1"
                     /translation="MPDPSIVWFRQDLRLADQHALAAAAEEGAVIPVYILDDETPGDW
                     RIGAAQRWWLHHSLAALAQSLERRGSRLILRRGRCADELQKLCEETGATRIHAIRHYE
                     PWWRRAEAEAAERLDLRLHGGNQLAPPERVRNGAGQPFKIYTPFWRALQQHLPPPPPL
                     GPPERLRAPKSWPQSERLVGWHLLPTRPDWAAEFPTLWSPGETQARVRLETFAPGVAD
                     YLERRNLPSEPGTSLLSPHLHFGEISPAAIFHAAAQAGEEAGSKFVKELAWRDFTTGQ
                     MAAHPEIGERHARATFDAFPWRRAGDAAEDFRAWMEGRTGYPIVDAGMRQLWRVGWIH
                     NRVRMIAASFLVKHLLIDWRDGARWFWDTLVDADYGNNSVNWQWIAGSGFDSNQFVRI
                     MAPLTQSAKFDAAGYIRHWVPELSGLADSEIHDPEASGARPSAYPPKIIGHKQARARA
                     LAAYAAVGG"
     gene            3224..4489
                     /locus_tag="VF589_12580"
     CDS             3224..4489
                     /locus_tag="VF589_12580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8258458.1"
                     /translation="MGVVNQESRGRHLVAADRGFATGGGLVARLFGGGFRRILDRIDA
                     GLAEGGIDATLPDGSKRLLGGRAPGPVPVVRLHSWRSLVRLVSSGSVGWYKAWALGEW
                     SSPDPVPLFDLFMRNGESLGQVARAKGIWRSVNRLAHLLRRPSKARARSNIAFHYDLG
                     NDFYRAWLDEGMTYSSALFAPGDRLEAAQARKLHALLDRLDLKPGQRLLEIGCGWGSL
                     AEMAARDYGVHVTGLTLSAEQKEWADARIARAGLSDRVAVELVDYRDAAGRYDAVASV
                     EMVEAVGEDYWPAYLQTIARMLQPGGRAALQLISIREELFDAYAASADFIQTYIFPGG
                     MLVSEPRFQAVAELAGLSWRDRTGFGLDYAETLKRWRERYDAAVEAGTLPPGFDRRFH
                     DLWRYYLMYCEGGFRGRGIDVAQITLVKV"
     gene            complement(4573..5283)
                     /locus_tag="VF589_12585"
     CDS             complement(4573..5283)
                     /locus_tag="VF589_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8258459.1"
                     /translation="MARSLEGRLALVTGASRGIGAATAEALAAAGAHVVITARTAAGL
                     EQVEDRIHAAGGSATIAPLDLTDGDSIARLAAAVAERWEALDVMVLNAATLGSLSPVP
                     AIDGKEFNKVLTLNLVAQQALIAAFDPLLRRSPRGRLLAVTSSVGRHPRAFWGGYGAS
                     KAALEALVLSYADETRNLGSPRVAIVDPGATATMMRARAFPGEDPAGLKPPSVVAEAI
                     AELIERDFETGYRLEVRG"
     gene            complement(5283..6740)
                     /gene="purF"
                     /locus_tag="VF589_12590"
     CDS             complement(5283..6740)
                     /gene="purF"
                     /locus_tag="VF589_12590"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX8258460.1"
                     /translation="MLTTNPFDDDKLREECGVFGVWGADTAAAVVALGLHALQHRGQE
                     AAGITSFDGRSFHSHRAQGHVAGNFDKDGVIRSLAGRASIGHVRYSTTGETALRNVQP
                     LFAELASGGFAVAHNGNISNAMRVRRELNRRGSIFQSTSDTEVIIHLVATSTYRTLLD
                     KFIDALKQVEGAYSLVCLTGEGMLACRDPLGIRPLVMGRLGDAYIFASETVALDVVGA
                     TYIRSVEPGELIIVSDRGFKSLRPFADVSPRPCIFEHVYFARPDSIVDGSSVYSVRKR
                     IGAELAIENPVEADYVVPVPDSGVPAAIGYAQQSGIPFELGIIRSHYVGRTFIQPSDQ
                     VRHLGVKLKHNANSALIQGKSLVLIDDSIVRGTTSLKIVQMLRDAGAREVHMRIASPP
                     TRHSCFYGVDTPERAKLLAAQMNVAEMADYIKADSLAFLSIDGLYRALGEDMREEAQP
                     QHCDACFTGDYPTRLTDHEETEPTDQLALLAERYG"
     gene            6925..7425
                     /locus_tag="VF589_12595"
     CDS             6925..7425
                     /locus_tag="VF589_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258461.1"
                     /translation="MRKLVLACLILAGCTGEESGGNQAAGAGASGGRSGTGRITTLTG
                     LYEGGESDRKDQLCMVEGEGGSTRFGLVVWGSEMHSCSGSGTAVRQGNSLRLAMAGDE
                     ACALTASIAGAMITLPAAIPDGCAYYCGARATLQGASFTQAGSTAEDAAKAKDLVGEP
                     LCGGGD"
     gene            7551..8921
                     /locus_tag="VF589_12600"
     CDS             7551..8921
                     /locus_tag="VF589_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258462.1"
                     /translation="MHVISTDRTTALSLTTASDEVFILPGVKFAAFSDWAAIYGDAIS
                     LEITNFGNVFGFNYGMQFYVSASGGNIANSAGAIIAGGSTGLSLESAFTYISNGGTIS
                     GGSGAAVEMFGNWQSLHNLAGGTLRGGLDGVLATGECEIFNDGTISARGVGVRINGFS
                     EISNAGSIFGSGEYGVVIQGSWSSLTNSGYIGGSHVGVYVVADTGETRIYNSGTIEGI
                     YIESSSTSPTFIFNSGELHGVYNMYSGPVVLRNSGDIIGDVVGGYDTQDTIRNIGTIA
                     GEVRLQGGNDIFNGAGGTVTGSVDGGAGNDRLTAGDHDVVRGGLGNDLLRGAGTMDTL
                     IGGRNADRMFGGGGADTFLFESHLESGATNGTADRILLFDRNQDIIDLELVDADSVAA
                     GNQEFTFIGTASFSNVAGQLRAVNAGANVFLQADLDGNGTTDFQVILGGLSGQAAQFT
                     AEDFVL"
     gene            complement(8999..11206)
                     /locus_tag="VF589_12605"
     CDS             complement(8999..11206)
                     /locus_tag="VF589_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258463.1"
                     /translation="MAFIKGTNLSENVNGTDDDDYILGLGGNDAANGGQGADNVYAGE
                     GADTLYDLVDGGAGDALYGEGGDDNFWMFDGEADIIDGGYGSDLYLTFGGTSVVIDLM
                     NQDLNAGIAAGDRLRSIERFLGGTANDTFRGSTRNEDFIDSQGDDFYNGRGGIDIYRS
                     GNPMGAADAVDFEVAYGNRAEELAALKGITLSSPQAGVAAYTIWNDANNDTVRDADEV
                     TYQVDELVGIEHYIGLYANSVMTGSAVNEVFYCNSAITSVDGAGGANDFVAYGGLATS
                     SAVTIDLEAGTAESFLAMGTLSRIESVVGSANGDEIRGSTLANTLVGLTGDDALDGCG
                     GNDRLDGGAGIDLLVGRNGNDILMGGALGDTIDGGVGSDTVCYTSATAAVTVTIATGA
                     TGGGEGDGDWFVSVENVIGSTFDDYVYGNGFANRIEGREGDDRLAGGAGNDRIYGDFD
                     PASAVAAGTASFDPAAVIGDSNSVEPGPVPDDSNQLYGQAGNDLLFGGSASDNLYGGS
                     GNDRLDGGTGSNNLYGGTGNDTYVIDYDNNYFTELAGQGTDVVLSSVSTYLRQHVENA
                     VATGAGAIMIEGNDLDNMITGNNAANELEGSGGSDSLIGGGGNDFLDGEYDSDTLTGG
                     GGKDTFAFQDDIREGGVDNITDFVAADDTILLSRNYFGGIMTEGRLSASAFRLGTEAA
                     DASDRIMYDQGTGRIFYDADGAGGSQAAVLFATVDAGTTLTAADFLTIQGYWD"
     gene            complement(11426..11851)
                     /locus_tag="VF589_12610"
     CDS             complement(11426..11851)
                     /locus_tag="VF589_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8258464.1"
                     /translation="MPHEAPEPVGLYEPFVRAGKLVALSAISSARDGMLVTGKVGREL
                     DLASGQRAARYAADNLLAVLRDAAGGDLAGVEQVLLVRGYVNAADDFPFVHKVIDAAS
                     EAVVAALGERGRHARTALGCATLPNNNAVTLDAVAVIAG"
     gene            complement(11858..12013)
                     /locus_tag="VF589_12615"
     CDS             complement(11858..12013)
                     /locus_tag="VF589_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258465.1"
                     /translation="MAYGVALLPFFVFDYYFYAVEPMLTAVGLLDAAGNVAMLALCWV
                     GWRTAER"
     gene            complement(12038..12577)
                     /locus_tag="VF589_12620"
     CDS             complement(12038..12577)
                     /locus_tag="VF589_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258466.1"
                     /translation="MSTFEFVFSLYGLLLGLSLAEVLGGFARAMRGHRAAPVGFQSPL
                     LGLIVMLDLTAFWLLVWSVRDVLAPNYRTLVVGLLISGLYYLAATMIFPQEGHDEPDL
                     DAHYQRNKMKVLGALLLCNLAAYLAVVAVGAAPFDVPKMLHMGGFAALVAVLIALRNK
                     AANIVLLCVICAWYFAAML"
     gene            complement(12630..12941)
                     /locus_tag="VF589_12625"
     CDS             complement(12630..12941)
                     /locus_tag="VF589_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258467.1"
                     /translation="MFDQDRISFDPRTGRPQPPVAWKVIGLEVVFGWLTAAIFLSSGV
                     FVARQGAWGWCAACLLMAALILLSVQWRKKQVDRAKAAFEKQLAAYERGLDAMLRQDR
                     P"
     gene            complement(13000..13362)
                     /locus_tag="VF589_12630"
     CDS             complement(13000..13362)
                     /locus_tag="VF589_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258468.1"
                     /translation="MPARLPIAFAATPPTPMELWTGGDDGLTQRLADAVREEFRQSGR
                     FALVEAGGDAGALRVGIPTHVAWQVVEGRTRLTYRLELERGGRRVAATGGSCWEDELD
                     RCARMLVRTVAGGSKPVG"
     gene            complement(14155..14352)
                     /locus_tag="VF589_12635"
     CDS             complement(14155..14352)
                     /locus_tag="VF589_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258469.1"
                     /translation="MLHLERLVDLDARPQLDYDLVEVPFPAEGEAIPAHCHMDDWPIG
                     DSLYGSEADAEREAAWLIRSL"
     gene            14634..16160
                     /locus_tag="VF589_12640"
     CDS             14634..16160
                     /locus_tag="VF589_12640"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8258470.1"
                     /translation="MRTSRSLRLPARLFCLAALTLPLPAQARQEPRSAAASTGQDRAA
                     TAESRGDAWRPVVQGRLANGLSYAILPRRSREPGAGLLMRIEGGFLAERRPGERGLAH
                     LIEHVALISPTTGAPDDLRHFMNVGLPLTFPAASAGTTIWSETNYFFSTRTTKPGDLD
                     TLLGLLREVAGDLIFRPDIVDAQRADVMREMAGRKPGNVVNARLIAAVAPGSPPDLLG
                     GQNSDDVPTTSLDTIRGLYRRLYRPENLMIAIVGDVDPAQMQALIEKRFGKWRGAGPA
                     PVRPSVPRFRADRIAPIGWSAAQAAPAVAMITLTLPTPPPARTRARQAEAMLMDAVAV
                     RAVINRLALAQPGSPPNKFGLFIENGEYGHRLFLIWDNFAPGPWRPAIAGLNELTCAL
                     SGAGLSEPEWAAAKRDVLRELEGRAGAMGEAPNVELAKDLSHALADGRHLIPPDEMLR
                     HARARLPAMSAAAMNRWWRRQWSAGAQHIRVESPELARVGDPKAAISEAIAGAGCKLR
                     "
     gene            complement(16228..16464)
                     /locus_tag="VF589_12645"
     CDS             complement(16228..16464)
                     /locus_tag="VF589_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258471.1"
                     /translation="MRTWIRGRLPWFLIDMGVAGFGEDCGKLGAQHAWCNLDDEHSRC
                     MFCGSEREGQLWRTNPYAASTIWHDLRNAPRFKR"
     gene            16634..17377
                     /locus_tag="VF589_12650"
     CDS             16634..17377
                     /locus_tag="VF589_12650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX8258472.1"
                     /translation="MDERWAEVDTYFAERLAPSDPVLGAVLAANAEAGLPPHDVSPLQ
                     GRFLELMVRLTGASRILEIGTLGAYSTLWMARALPPGGRIVTLETDPRHAGVARANLA
                     LAGVADRVDLRAGPALETLPGLDDLAPFDLVFIDADKPSNPDYLAWALRLTRPGSLIV
                     ADNVVRGGAVADAASGDPRVHGIRRFTDLIAAEPRLIATAVQTVGSKGWDGFAIALVV
                     DPHREPFPLAEKPGSALSQRRVGDDRGLD"
     gene            17378..17950
                     /locus_tag="VF589_12655"
     CDS             17378..17950
                     /locus_tag="VF589_12655"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX8258473.1"
                     /translation="MGNFFRVIAWHLKSLLRFKGRDDRPTFWPYAGSVVGLAGLVMMA
                     AMFPVFRGVADFAQQHPDRTSIETGPGRYLVTIEGPPAGLDGAMSNLTSAVNLLFGCC
                     IGLLAAAVARRLHDTGRTGLWGLMPVPFILFSGAMMPRMFAEDGPRSDLFFAVFVSNW
                     LYLAALAWLALLLAKPGDPAGNRYGPGKTA"
     gene            18017..18160
                     /locus_tag="VF589_12660"
     CDS             18017..18160
                     /locus_tag="VF589_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258474.1"
                     /translation="MRFDVQAGAGRIDFGSGGHDQAVRLSPACCALLLDDRLTGFWFS
                     LQR"
     gene            complement(18204..18596)
                     /locus_tag="VF589_12665"
     CDS             complement(18204..18596)
                     /locus_tag="VF589_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein tyrosine
                     phosphatase family protein"
                     /protein_id="HEX8258475.1"
                     /translation="MAVDDGSGVPRIADVRHAVPIAPMKKILFLCSQNRLRSPTAEQV
                     FASHPGIEVASAGLNNDAENPTTPELIAWADLIFVMEKAHRNRLQKRFRQHLKARVIC
                     LDIPDEYEFMDKSLISLLKAKVSRHLPI"
BASE COUNT         2971 a         6553 c         6021 g         3081 t
ORIGIN      
        1 ccggtgacca aggtatccgg ccagcccttc aagatcgcgg agatcaccga cgcgatcgag
       61 gcggcgctct catgagtagt ccgcgcctgc tgccgctcct tcttatgctt gatgccgtcg
      121 tggccgatca ttttccttcg aggctgacga caatgaacga aatgaccaag ctcaccgcaa
      181 aggatttcgc caccgaccag gaggtgcgct ggtgccccgg ctgcggcgat tatgcggtgc
      241 tgaaggccgt gcagcggacc atgcccgaac tcggcgtccg gcccgagaac gtcgtcttcg
      301 tgtccggcat cggctgctcg tcccgcttcc cttattatat ggaaacctac ggcttccata
      361 cgatccacgg ccgcgcgccg accttcgcga ccggcatcaa gctcgccaat cccgagctcg
      421 acgtctggat catcaccggc gacggcgacg cgctgtcgat cggcggcaac cacaccatgc
      481 acgtgcttcg ccgcaacctc gattgccaga tactgctttt caacaacgag atttacggcc
      541 ttacaaaggg ccaatattct ccgacctcgc gggtcggcac gcgctcgccc tcgacgccgt
      601 tcggctcggt cgaccggccg gcttcccctt gcgccttcgc gctcggctcg ggcgcgcggt
      661 tcgtggcgcg cgccatcgac gtcaacaaga atttgcccga cgtgctcaag gccgcccatg
      721 cccataaggg cgccgccttc atcgagatct tccagaattg catcgtctat aatgacgacg
      781 tcttcgcccc cttcaccgcc aaggacaatg ccggcaacca gctctggctg aaggccggcg
      841 agccgatgct gttcgccggc ggcgccaagg gcatcacgct cgaccgcgag gcgctgaccc
      901 tgcgggtggt ggaggtggcg gacggcgact ggcaggccgc cggcgtcatc gtccacgagc
      961 ccgccaaccg ctcggttgcc cacatgctgg tcgaaatgcc gttcggcgcc ttcccgatgg
     1021 cgctcggcgt catctacgac gatccgcggc cgactttcga aagcgccgtc gtcgcccaga
     1081 atgccgaagc cagcgccggc aaggtcccca acctccaggc tttggtcggg aagggccaga
     1141 gctgggaagt gaccaaggaa ccgcacgtca tatgatggga ttggcggccg ttctgctcat
     1201 tgtcgggcag gggcctgcct tcaaagtcgt ggaaacgccg ccgccgccgg acgggcaaac
     1261 gccgccgccg atcgtcaaag gctcccccga cggggcgatc atgcgggtgc cgcgcctcga
     1321 tccggcagta gcactgagga gtcgcggctt ttccgagggt gcgatccgga ccatcatggc
     1381 cgcggcccgt acgcggatgg cggcgcggcc cgcgcagatg catatgatcg agcggctgaa
     1441 gcaggagttg gcggtagaga ggtcttccga ttccccgagc gccgatcgga ttgaagcgct
     1501 gcttcgtcgg gcggctgcgg caagctccgc tgacctgagc gagcgcgtcg gcaggacgct
     1561 ccgactcctc cgggcgctcc cgccagccga tctgaagctg tacctgcgag ccttcggtcc
     1621 cgacgagccg gagttcctgg ccctgtcggt gattccgact gtccgctgat cgcccgagga
     1681 caatgcccga tccatccatc gtctggtttc gccaggacct ccgcctcgcc gatcagcacg
     1741 cgcttgccgc ggcagcggag gaaggcgcgg tcatccccgt ctacatcctc gacgacgaga
     1801 cgcccggcga ttggcggatc ggggcggcgc aacgctggtg gttgcaccac tcgctcgccg
     1861 cgctcgcgca gagcctcgag cggcgagggt cgcggctgat cctccgccgc ggacgctgcg
     1921 ccgatgaact tcagaaactg tgtgaggaaa ccggggcaac tcgcatccat gccatccgcc
     1981 attacgagcc atggtggcgc cgcgccgagg cggaggcggc ggagcggctc gatctacggc
     2041 ttcatggcgg caaccaattg gcgccgccgg agcgagtgcg gaacggggcc gggcagccgt
     2101 tcaaaatcta cacgcccttc tggcgcgcgc tgcagcagca tctgccgccg ccgccgccgc
     2161 tcggcccgcc ggagcggctc cgggccccga agtcatggcc ccaaagcgag cggctcgtgg
     2221 gttggcactt gctgccgacg cggccggact gggctgccga gttcccgacg ctttggtcgc
     2281 ccggcgagac gcaggcgcgg gtacggctgg agacgttcgc gcccggcgtc gccgactatc
     2341 tcgagcgccg caacctgccg tccgagccag gcacctcgct tctgtcgccc cacctccatt
     2401 tcggcgaaat ctcgcccgcc gcgatcttcc acgccgccgc gcaagcgggc gaggaggccg
     2461 gctccaagtt cgttaaggag ctcgcctggc gcgatttcac caccgggcag atggccgcgc
     2521 atccggagat cggcgagcgg cacgcgcgag ccacgttcga cgccttcccc tggcgccgcg
     2581 ccggcgacgc cgcggaagat ttccgcgcct ggatggaggg gcgcaccggc tatccgatcg
     2641 tcgatgccgg catgcgccag ctctggcggg tcggctggat ccacaatcgc gtccgcatga
     2701 tcgcggcgag tttcctggtg aagcacctgc tgatcgactg gcgcgatggc gcccgctggt
     2761 tctgggacac tttggtcgac gccgattacg gcaataacag cgtcaactgg cagtggatcg
     2821 ccggctcggg cttcgattcc aaccagttcg tgcggatcat ggctccgctg actcaaagcg
     2881 ccaagttcga cgcggccggc tatattcgcc actgggtgcc ggagctttcc ggcctcgcag
     2941 actcggagat ccacgatccc gaagcgtccg gcgcccgccc gtccgcctac ccccccaaga
     3001 tcatcggcca caaacaggcc cgcgcccgcg cgctcgccgc ttatgccgct gtgggcgggt
     3061 gagggaaggg aagggaaggg agagggtgcc atcgtcatcc cagcgaaagc tgggacctct
     3121 cttcctcccc ggcgatcgcc atcctcctcg gcgatcccct tcctcccccc gcgctcgccc
     3181 tttcttcact cccccgattg cgccccacgc cgcggcttgc cacatgggcg tcgtgaacca
     3241 ggagagccga ggccgtcatc tcgtcgccgc cgaccgcggt ttcgccaccg gcggcggcct
     3301 cgtcgcgcgg ctgttcggcg gcggcttccg gcgcatcctt gaccggatcg acgccggcct
     3361 cgccgaaggc ggcatcgacg ccacgcttcc cgacggctcc aaacgcctgc tcggcggccg
     3421 cgcgccgggc ccggtgccgg tggtccgcct ccacagctgg cgctcgctgg tccggctggt
     3481 gagttcgggc tcggtcggct ggtacaaggc ctgggcgctg ggcgaatggt cgagccccga
     3541 cccggtgccg ttattcgacc tcttcatgcg caatggcgag agtctcggcc aagtcgctcg
     3601 cgccaaaggc atttggcgtt cggtcaatcg cctcgcccat ctcctccgcc gccccagcaa
     3661 ggcgcgtgcc cgctccaaca tcgccttcca ttacgatctc ggcaacgatt tctaccgcgc
     3721 ctggctcgac gagggcatga cctattcgag cgccctgttc gcccccggcg acaggctcga
     3781 ggccgcccaa gcccgcaagc tccacgccct gctcgaccgg ctcgacctta agcccggcca
     3841 gcggctgctc gagatcggct gcggctgggg ctcgctggcc gagatggccg cccgcgatta
     3901 cggcgtccac gtcaccggcc tcactttgtc ggcagagcaa aaggaatggg ccgacgcccg
     3961 catcgcccgc gccggcctgt cggaccgcgt cgccgtcgag ctcgtcgact atcgggacgc
     4021 cgccggccgc tacgacgccg tcgccagcgt tgagatggtg gaagcggtcg gcgaggatta
     4081 ttggcccgcc tacctccaga ccatcgcccg catgctgcag cccggcggcc gcgccgcgct
     4141 ccagctcatc tcgatccgcg aggagctgtt cgacgcctat gccgccagcg cggacttcat
     4201 ccagacctac atcttccccg gcggcatgct ggtctcggag ccgcgcttcc aggcggtcgc
     4261 cgagctggcc ggcctcagct ggcgcgaccg caccggcttc ggcctcgatt atgccgagac
     4321 gctgaagcgc tggcgcgagc gctacgacgc cgccgtcgaa gcgggcacgc tcccgcccgg
     4381 attcgaccgc cgtttccacg acttgtggcg ttattatctg atgtattgcg aaggcggctt
     4441 ccgcggccgc ggcatcgacg tcgcccaaat caccttggtg aaagtctgat ccttccggcg
     4501 gaagtaaccc acgtcacccc agcgaaagcc ccctcccacg tcatcccagc gaaagctggg
     4561 atctcccttc tcttaacccc gaacctcgag ccgatacccg gtctcgaaat cccgctcgat
     4621 gagctccgcg atcgcctccg ccaccacgct cggcggcttc aaacccgccg ggtcctcgcc
     4681 cggaaacgcc cgcgcccgca tcatggtcgc ggtcgcgccg ggatcgacga tcgccaccct
     4741 cggcgacccc aggttccgcg tctcgtcggc atagctcagc accaaggcct ccagcgccgc
     4801 cttggacgcg ccatagccgc cccagaaggc gcgcggatgc cgcccgacgc tcgacgtcac
     4861 cgccagcagc cgcccgcgcg ggctgcgccg cagcaacgga tcgaaggccg cgatcagcgc
     4921 ctgctgggcg acgaggttca aggtcagcac cttgttgaat tccttgccgt cgatcgccgg
     4981 caccggcgaa agcgagccga gcgtcgccgc attcagcacc atgacgtcga gcgcctccca
     5041 gcgctcggct accgccgccg ccagccgggc gatgctgtcg ccgtcggtaa ggtccaaagg
     5101 ggcgatcgtc gcgctgccgc cggccgcgtg gatgcggtcc tcgacttgct cgagcccggc
     5161 cgcggtccgc gccgtgatca cgacatgcgc cccggccgcg gcgagcgctt ccgccgtcgc
     5221 cgccccgatc ccccggctgg cgccggtgac gagggccagc cggccttcca gcgatcgagc
     5281 catcagccgt agcgttcggc cagcaacgcc agctggtcgg tcggctcggt ttcctcatgg
     5341 tcggtgagcc gggtcggata gtcgccggtg aagcaggcgt cgcaatgctg cggctgcgct
     5401 tcctcgcgca tatcctcgcc gagcgcgcgg tagagcccgt cgatcgaaag gaaggcgagg
     5461 ctgtcggcct tgatataatc ggccatctcg gcgacgttca tctgcgccgc cagcaatttc
     5521 gcccgctccg gcgtgtcgac cccgtaaaag cagctgtgcc gcgtcggcgg cgaggcgatc
     5581 cgcatatgga cttcgcgcgc gccggcgtcg cgcagcatct gcacgatctt gaggctggtc
     5641 gtcccgcgca cgatcgaatc gtcgatcagg accaagctct tgccctggat cagcgcgctg
     5701 ttggcattgt gcttcagctt gacgccaaga tggcggacct ggtccgacgg ctggatgaag
     5761 gtgcgcccga cataatgcga gcggatgatg ccgagctcga acgggatgcc cgattgctgg
     5821 gcatagccga tcgccgccgg cacgcccgaa tcgggcaccg gcacgacata atcggcctcg
     5881 accggattct cgatcgccag ctcggcgccg atccgcttcc tcaccgaata gaccgaggag
     5941 ccgtcgacga tcgaatcggg ccgcgcgaaa tagacatgct cgaagatgca cggccgcggg
     6001 ctcacgtcgg cgaacggccg cagcgacttg aagccgcggt ccgacacgat gatcagctcg
     6061 cccggctcga ccgagcgaat ataggtcgcg ccaacgacgt cgagcgccac cgtctccgag
     6121 gcgaagatat aggcgtcgcc gagccgcccc atcaccagcg gccggatgcc gagcgggtcg
     6181 cggcaggcga gcatgccctc gccggtgagg cagaccagcg aataggcacc ctcgacctgc
     6241 ttcagcgcgt cgatgaactt gtcgagcagg gtccggtagg tcgaggtcgc gaccaagtgg
     6301 atgatgacct cggtgtcgga ggtcgactgg aagatcgatc cgcgccggtt gagctcgcgc
     6361 cgcacccgca tcgcgttcga gatattgccg ttatgcgcca ccgcgaaccc gcccgaggcg
     6421 agctccgcga acaagggctg gacgttgcgc aacgccgtct cgccggtggt cgaatagcgg
     6481 acatggccga tcgaggcgcg gccggcgagg ctgcggatca cgccgtcctt gtcgaaattg
     6541 ccggcgacat ggccttgggc gcggtggctg tggaagctgc gcccgtcgaa gctggtgatg
     6601 ccggccgctt cctggccgcg atgctgcagc gcatggaggc cgagcgcgac cacggccgcg
     6661 gccgtatcgg cgccccagac gccgaagacg ccgcattcct cgcgcaattt gtcgtcgtcg
     6721 aaaggattgg tggtcagcat gttaccgtcc gcgcccatgt gctggtcccc gcccgaagcc
     6781 ctgctttcgc cccacgcgcc atatagggaa gcgcaaagcg tttgtcgccc tcgtgctgat
     6841 cgagttttcc gattaacgtg acagcgtggc ctgaataagg ccggagccgt gaaccgccgc
     6901 cgccccccgt gcattggagc gcccatgaga aagctcgtcc tcgcctgtct gattctcgcc
     6961 ggctgcacgg gcgaggaatc cggcggcaac caggcggccg gagcaggcgc ctcgggcggc
     7021 cgcagcggta ccggccggat taccactctg accggcctct acgaaggcgg cgagagcgac
     7081 cgcaaggacc agctctgcat ggtcgaaggg gagggcggct ccacccgctt cggcctggtc
     7141 gtctggggct ccgagatgca cagctgctcc ggctccggca ccgccgtccg ccagggcaat
     7201 tcgctccggc tcgccatggc cggcgacgag gcctgcgcgc tcaccgcttc catcgccggc
     7261 gcgatgatca ccctgcctgc cgcgataccc gacggctgcg cttattattg cggcgcccgc
     7321 gccacgctcc aaggcgcgtc cttcacccag gccggctcca ccgccgagga tgcggcgaag
     7381 gccaaggacc tggtcggcga gcccttatgc ggcggcggcg attgatctct gcccgcctat
     7441 agcaaggctg aacaaaacct tctttctgaa gcagttttgc cgccgcgact cgggggccgg
     7501 cgagttgccc atctgcgttg atttccatcg ggcccaaggg gggatgtccg atgcatgtga
     7561 tctcaaccga tcgaaccaca gctctttcgc tcacgacggc gagcgacgag gtgttcattc
     7621 tgccgggtgt gaaattcgcc gccttctccg actgggcggc gatctacggc gacgcgatca
     7681 gtctcgagat cactaatttc gggaacgtgt tcgggttcaa ctacggcatg caattctatg
     7741 tctccgccag cggcggaaac atcgccaact cggccggcgc gatcatcgcc ggaggctcta
     7801 ccgggttgtc gctcgaatcc gccttcacct atatctcgaa cggcggcacc atctcgggcg
     7861 ggtcgggcgc tgccgtcgag atgttcggca attggcaaag cctgcacaac ctcgccggcg
     7921 gcaccctccg cggcggattg gatggcgtcc tggccactgg cgaatgcgag atcttcaacg
     7981 atggcaccat atcggcgcgg ggagtcggcg ttcgaatcaa cggcttctcg gaaatatcga
     8041 acgccggttc gatctttggg tccggtgaat atggtgtcgt catccaagga tcgtggagct
     8101 cgttgacgaa ctccggttac atcggcgggt ctcacgtcgg cgtttacgtt gtcgccgaca
     8161 ccggcgagac tcgtatctac aacagcggga cgatcgaggg catctacatc gaatccagca
     8221 gcacttcgcc gacgttcata ttcaactcgg gcgagctgca cggcgtctac aatatgtatt
     8281 ccggacccgt cgtgcttcgc aacagcgggg acattatcgg cgacgtcgtc ggcggctatg
     8341 acacccagga caccatcagg aacatcggca ccatcgccgg cgaggtgcgc ctccagggag
     8401 gaaacgacat cttcaacggc gccggaggaa cggtaaccgg cagtgtcgac ggaggcgcgg
     8461 gcaatgatcg cctgacggcc ggggatcacg acgtcgtccg gggaggcctc ggcaacgacc
     8521 tgctccgcgg cgcgggaacg atggacacgt tgatcggcgg gaggaacgcc gaccggatgt
     8581 tcggcggcgg cggcgccgat accttcctgt tcgagtccca cctcgaatcg ggggcgacca
     8641 acggcaccgc cgaccggatc ctgctgttcg atcgaaacca ggacataatc gatctggagc
     8701 tcgtggacgc cgactcggtc gcggccggaa accaggagtt cacgttcatc ggaacggcga
     8761 gcttctccaa cgtcgccggc cagcttcgtg ccgtgaacgc gggagcgaac gtgttcctgc
     8821 aggcggatct ggacggcaac gggaccaccg acttccaggt gatcctcggc gggctgagcg
     8881 ggcaagccgc gcaattcacc gccgaggact tcgtgcttta ggctctgcgc cggcgcatga
     8941 cgcggggggc cgagcttcgc ccggcctccc gccctcatgc cgcatcaatg tcgcgggctc
     9001 agtcccaata gccctggatg gtcaggaagt ccgccgcggt gagcgtcgtg ccggcgtcga
     9061 cggtggcgaa caggacggcg gcttgcgagc cgccggcccc gtcggcatcg tagaagatgc
     9121 ggccggtgcc ctgatcgtac atgatgcggt cgctcgcgtc ggccgcctcg gtcccgagcc
     9181 ggaaggcgct ggccgaaagc cggccctcgg tcatgatccc gccgaaatag ttccggctca
     9241 gcaggatcgt gtcgtcggcg gcgacgaagt cggtgatgtt gtccacccct ccttcgcgga
     9301 tatcgtcctg gaaagcgaaa gtgtccttgc cgccgccgcc ggtcagcgtg tcgctgtcat
     9361 actctccgtc caggaagtcg ttgccgccgc cgccgatcag actatccgat ccgcctgagc
     9421 cttcaagctc gttcgccgca ttgttgccgg tgatcatgtt gtccaggtcg ttgccttcga
     9481 tcatgatcgc gccggcgccg gtcgccaccg cgttttcgac atgttggcgc agataggtgc
     9541 tgaccgagct gagcaccacg tcggtccctt gtcccgccag ctcggtgaaa tagttgttat
     9601 cgtaatcgat tacgtaggta tcgttgcccg tgccgccata aaggttgttg ctgcccgtgc
     9661 cgccgtcgag ccggtcgttg ccgctgccgc cgtacagatt gtcgctggca cttccgccga
     9721 acaggaggtc gttgccggcc tggccgtaga gctggttgga gtcatcgggc accggtcccg
     9781 gctcgacgct gttcgaatcg ccgatcaccg cggcaggatc gaagctggcg gttcccgccg
     9841 ccaccgcgct ggccgggtcg aaatcgccgt agatgcggtc gttgccggcg ccgccggcga
     9901 gacggtcgtc gccttcgcga ccttcgatcc ggttggcgaa gccgttgccg taaacatagt
     9961 cgtcgaaggt tgagccgatc acattctcga ccgacacgaa ccagtcgccg tcgccttcgc
    10021 cgcccccggt ggcgccggtc gcgatggtga cggtgacggc cgcggtggcg ctggtatagc
    10081 aaacggtgtc gctgccgacg ccgccgtcga tcgtgtcgcc gagcgctccg cccatcaata
    10141 tgtcgttgcc gttccggccg accagcaggt cgatgccggc gccgccgtcg agccggtcgt
    10201 tgccgccgca gccgtcgagc gcgtcgtcgc cggtcaggcc aaccagggtg ttcgccagcg
    10261 tgctccccct gatctcgtcg ccgttggctg agcccacgac gctttcgatc cggctaagcg
    10321 tgcccatcgc gaggaagctc tcggccgttc ccgcctcgag atcgatcgtc accgcgctcg
    10381 aagtggcgag gccgccatag gccacgaaat cgttggcccc gccggcgccg tcgacgctgg
    10441 taatggcaga gttgcagtag aagacttcgt tgactgcgga cccggtcatt accgaattgg
    10501 catagaggcc tatataatgc tcgatgccga ccagctcgtc gacctggtag gtgacctcgt
    10561 cggcgtcccg taccgtgtcg ttgttggcgt cgttccagat cgtataggcg gcgacgcccg
    10621 cctggggcga ggagagggtg atgcctttga gcgccgccaa ctcttctgcg cggttgccgt
    10681 aggccacttc gaagtcgact gcgtccgcgg cgcccattgg attgccggag cgatagatgt
    10741 cgatgccccc ccggccgtta tagaaatcgt cgccctgcga atcgatgaaa tcctcgttgc
    10801 gcgtgctgcc gcggaacgtg tcgttggcgg taccgcccaa aaagcgctcg atgctgcgca
    10861 ggcgatcgcc ggccgcgatc ccggcgttga gatcctggtt catcagatcg atgacgacgc
    10921 ttgtgccgcc gaacgtcaga taaaggtcgc tgccgtagcc gccgtcgatg atatcggcct
    10981 cgccgtcgaa catccagaaa ttgtcgtcgc cgccttcgcc gtaaagcgcg tcgcccgcgc
    11041 cgccgtccac cagatcgtac agagtatccg caccttcacc tgcatagacg ttgtcggctc
    11101 cctggccgcc attggccgcg tcattgcctc ccagtccaag aatatagtcg tcgtcgtcgg
    11161 tgccgttgac gttctcgcta agattagtgc ccttgatgaa tgccatgata tctcccctaa
    11221 gctaggatgg gtactggccg cagaaggccc tccaactcgc tctttacctg cctagcgccg
    11281 ccagcaactt agtccctcgg gggacgacca agccggggaa agcggcgacc aaatcccgcg
    11341 tgggcggcgg cgatgcgctt aacgggactt ctggtacgac cgggatgcag gcgggctggt
    11401 cgcctgcgcc tctgccagcg gcaggctagc cggcgatcac cgcgaccgcg tcgagggtga
    11461 cggcattgtt gttgggcagc gtcgcgcagc caagcgcggt ccgggcatgg cgcccccgct
    11521 cgccgagcgc cgcgacgacc gcttcggatg cggcgtcgat caccttgtgc acgaacggga
    11581 agtcgtcagc cgcgttgaca tagcccctga ccaacaggac ctgctccacg ccggccagat
    11641 cccctccggc cgcgtcccga aggaccgcca gcagattgtc ggccgcgtac cgcgccgctc
    11701 gctggcccga ggccaggtcc agctcgcgcc cgaccttgcc ggtgaccagc atcccatcgc
    11761 gcgccgagct tatcgccgac agcgcgacca gcttgccggc ccggacgaac ggctcgtaga
    11821 ggccgaccgg ctcgggtgct tcatgcggca tcggcgctca ccgctcggcg gtccgccagc
    11881 cgacccagca caaagccagc atcgccacgt tcccggccgc gtcgagcagc ccgaccgcgg
    11941 tcagcatcgg ctccaccgca tagaaataat aatcgaacac gaagaaaggc agcagcgcga
    12001 cgccgtacgc cacgaacgtc tttttggcgg gccggtccta gagcatcgcc gcgaaatacc
    12061 aggcgcagat cacgcacagc aaaacgatgt tcgccgcctt gttccgcaac gcgatcagca
    12121 ccgccaccag cgccgcgaac ccgcccatat gcagcatctt cggcacgtcg aacggcgccg
    12181 cgcccaccgc caccaccgcc agataggcgg caaggttgca cagcagcagc gcgcctagca
    12241 ccttcatctt gttgcgctgg taatgcgcat cgagatcggg ctcatcatgc ccctcctgcg
    12301 ggaagatcat ggtggccgcg agataataga ggccgctgat cagcagcccc acgaccaagg
    12361 tccggtaatt gggagcgagc acgtcgcgca cgctccacac caacagccag aaggcggtga
    12421 ggtcgagcat cacgatcagg ccgagcagcg gcgactgaaa cccgaccggc gcggcgcgat
    12481 gcccgcgcat cgcccgcgca aagccgccaa gcacctccgc cagcgacaat cccagcagca
    12541 gcccgtacag cgagaaaacg aattcgaagg tgctcatcgc gtcccccgcc gcaccattcc
    12601 atgtccagcc acggtccgca atgggcggcc taaggcctgt cctgacgcag catggcgtcg
    12661 aggccgcgct cataggcggc gagctgtttt tcgaatgcgg ctttggcccg atcgacctgc
    12721 ttctttcgcc actggacaga cagcagaatg agcgccgcca tgagcaggca agccgcgcac
    12781 catccccacg cgccttggcg ggcgacgaag acgcccgagg agaggaagat cgccgccgtc
    12841 aaccagccga agaccacctc caagccgatc accttccacg ccaccggcgg ctgcgggcga
    12901 ccggttcgag ggtcgaagct gatccggtcc tgatcgaaca tcgcatggac tttgcctgca
    12961 aatcggctgc gtccgcaatg ggcggagagg gagggccgct cagccgaccg gtttggagcc
    13021 gccggcaacc gtccgcacca gcatccgcgc acagcggtcg agctcgtctt cccagcagct
    13081 tccgcccgtt gcggccaccc gccgcccccc gcgctccagc tccagccggt aggtgaggcg
    13141 ggtccgtccc tccacgacct gccaggcgac atgggtgggg atgccgaccc tcagcgcccc
    13201 ggcatcgccg cccgcctcga ccagcgcgaa gcggccggac tggcggaact cttcccgcac
    13261 cgcatcggcc agccgctggg tcaggccgtc gtcgccccct gtccacagct ccatcggagt
    13321 gggcggcgtc gcggcgaagg cgatagggag acgcgcaggc actcaggcac agcggcagga
    13381 gaagggcagg ccaggctttc acggcgcaga gcttcgcatt cccctcgcac ctcggcaatg
    13441 gccgccggct ggctgcaggg cggcgtccag cgggtggctc cctcacctcg cgggcactca
    13501 ctccagcacc ttcgctccca agacccggtc gacgacgacg atgtgtcggg ccagccccat
    13561 atcgccgtaa ccgcccgcaa cagccgttcg tctgccgatg aagtccagat agacgcacaa
    13621 ccctcgctgc atccgctcgg tagagggcga gggggaaggc tgggatggtg ctccacccaa
    13681 aagccgctcc gcgccccgcg cggaggtggc gtttgccgcc ccggatagaa gcggccgctc
    13741 tcggtatacc tgacggcgac cccgcgcatg cgctgaggat cggtgaatct gaagcaatcc
    13801 gcatcgggaa ggcggcccgg tttaccgtcg atcgttctga agcacggctc gaaatcggca
    13861 tggatcatgg gaagcgggga ggcggcgaag aactggtcgc gcgggtgctt gtaggcggag
    13921 gggggtgtcc cggccccaca tccggccaga aggaaaccgg cggccgtaag gaaggcgcac
    13981 gctctcatcg cggcgggatc gtcatggcgc ccgaagcccg tcggcgaagc tcgcttcccg
    14041 tgccatcggc cgatcctggc acgtcgccat tcccatgccc aagcggaggc caactcctct
    14101 gccccgcgac tgcggccccc cgatcagcgg ggcgacggtc acgcgccttt gacttcagag
    14161 gctgcggatc aaccacgcgg cctcgcgttc ggcgtcggct tccgacccgt agagcgagtc
    14221 gccgatcggc cagtcgtcca tatggcaatg cgccgggatc gcctcgcctt ccgccgggaa
    14281 cggcacctcc acgagatcat agtccaactg cggcctcgca tcgaggtcta ccagacgttc
    14341 gaggtggagc atgtaaagcc catccgcccg tcggcgaacc cttagccgct tgcatccgtc
    14401 atcggaggtg gcgacgattc ttcccgcgtc catatggttc tgtgttgccg gtccggccgg
    14461 tgtccgcaac cggccgaacg cggcccgttt tcgcactatc ggcttcatgc ccacggcccc
    14521 gttttgctca cggcgcgccg gccatcctat tgcctcaaag ggcgacgatg gcgggcatgg
    14581 tccggcgcgc acggatcggc tgttctcgcg gcgccccggc aggagatcgg acaatgcgga
    14641 cctcccgttc cttgcggctg ccggcgcgcc tgttctgcct ggccgcgctt acgctgccgc
    14701 tgcccgcgca agctcgacag gagccccgga gcgccgccgc ctccaccggc caggatcggg
    14761 ccgccaccgc ggagtcgcgc ggcgatgcgt ggcgcccggt ggtgcagggg cggctggcga
    14821 acggcctcag ctacgccatc ctgccgcgac gctcgcgcga gccgggcgcc gggctgctga
    14881 tgcggatcga gggcggcttc ctagccgagc ggcggccggg cgagcgcggc ctcgcccacc
    14941 tgatcgagca tgtcgcgctg atcagcccga ccacgggcgc acccgacgac ctgcgccatt
    15001 tcatgaacgt cggcctgccg ctgaccttcc cggccgcctc cgccggcacc acgatctgga
    15061 gcgagaccaa ttatttcttc tcgacccgta cgacgaagcc cggagacctc gacaccttgc
    15121 tggggctgct ccgagaggtc gccggagacc tgatcttccg gcccgacatc gtcgatgcgc
    15181 agcgcgccga cgtgatgcgg gagatggccg ggcgcaagcc cgggaacgtc gtcaacgccc
    15241 gcctcatcgc ggcggttgcg ccggggtcgc cccccgacct gctcggcggt cagaattcgg
    15301 acgacgtgcc gaccacgagc ctcgacacca tccgcggcct ctaccgccgg ctctaccggc
    15361 ccgaaaattt gatgatcgcc atcgtcggcg acgtcgaccc ggcccagatg caggcgctca
    15421 tcgagaaacg gttcggcaaa tggcggggcg ccggtcccgc tcccgtccgc ccgtccgtcc
    15481 cgcgcttccg ggccgatcgc atcgcgccga tcggctggtc agctgcccaa gcggcgcctg
    15541 ccgtcgcgat gatcaccctc accctgccga ctccgccgcc ggcgaggacg cgcgcgcggc
    15601 aggcggaagc gatgctgatg gacgcggtgg cggtccgcgc ggtcatcaac cgcctggcgc
    15661 tggcccagcc gggcagcccg cccaacaagt tcggcctgtt catcgagaat ggcgaatatg
    15721 gccaccgcct gttcctgatc tgggacaatt tcgcgccggg tccgtggcgg ccggcgattg
    15781 ccggcttgaa cgagctcacc tgcgcgctca gcggggccgg cctgtccgaa cccgaatggg
    15841 cggcggcgaa acgggacgtg ctccgggagc tggagggccg cgccggagcc atgggcgagg
    15901 cgcccaacgt cgagctggcg aaggacctgt cgcacgcgct ggccgacgga cggcacctga
    15961 tcccgcccga cgagatgctg cgtcacgccc gcgcccggct gccggcgatg agcgctgcgg
    16021 cgatgaaccg ctggtggcgc cgccaatgga gcgccggcgc ccagcacata cgcgtcgagt
    16081 cgcccgagct cgcgcgcgtc ggcgacccca aggcggcgat aagcgaggcc atcgccggcg
    16141 ccggctgcaa gcttcgctga tccgcaatcc ccatcgcggc gggtaatcgc gagacgccgg
    16201 gaacgcccgc gacgggagag cggacgttca ccgcttgaag cggggcgcat tcctcaaatc
    16261 gtgccagatc gtcgaggcgg cgtaggggtt ggtgcgccaa agctgcccct cccgttcgct
    16321 cccgcagaac atgcagcggc tatgctcgtc gtcgagattg caccaagcgt gctgagcgcc
    16381 cagcttgccg caatcctcgc cgaagcccgc cacgcccatg tcgatcagga accagggcag
    16441 ccgtcccctg atccacgtgc gcaaggcgtg ggggtgatcc ccaaattctt ctcgaagact
    16501 cgaccgccac cccatgttct ggagtgtgcc acctgatgcc gaacatccgc aatgggccga
    16561 gcggcggatg cggccgctct tgcgcggcgc ggccaggcct gctgatgtcg gccgcttccg
    16621 agagggtgac gcgatggacg agcgctgggc ggaggtcgat acctatttcg ccgagcggct
    16681 cgcgccctcg gacccggtgc tgggcgccgt gctcgccgcc aatgccgaag ccggcctgcc
    16741 gccgcacgac gtgtcgccgc tgcaggggcg ctttctggaa ctgatggtgc gcctgaccgg
    16801 cgcttctcgc atcctcgaga tcgggacgct cggggcttat tcgaccctct ggatggcgcg
    16861 cgcgctgccg cccggcggcc gcatcgtcac gctcgagacc gacccccgcc acgccggcgt
    16921 cgcccgcgcc aacctcgcgc tcgccggggt cgccgaccgc gtcgacctgc gcgccggccc
    16981 ggcgctcgaa accctgccgg ggctggacga tcttgccccg ttcgacctcg tgttcatcga
    17041 cgccgacaag ccttctaatc ccgattatct cgcctgggcg ctgcggctca cgcgtccggg
    17101 cagcctcatc gtcgccgaca atgtcgtgcg cggcggcgcc gtcgccgatg cggcaagcgg
    17161 cgacccgcgc gtccacggca tccgccgctt cacggatctg atcgccgccg agccgcggct
    17221 gatcgcgacc gcggtccaga cggtggggag caagggctgg gacgggttcg ccatcgcgct
    17281 cgtggtcgat ccccatcggg agccattccc gctggcggaa aaacctggtt cggccctatc
    17341 tcagcgccgc gtcggcgacg atcgaggatt ggattgaatg ggcaatttct tccgcgtgat
    17401 cgcctggcat ctgaaaagcc tgctccgctt caaggggcgc gacgaccggc ctaccttctg
    17461 gccctacgcc ggctccgtcg tcgggctggc cggcttggtc atgatggcgg ccatgttccc
    17521 ggtcttccgg ggcgtggccg actttgcgca gcagcatccc gatcggacca gcatcgaaac
    17581 cggccccggc cgttatctgg tcacgatcga gggtcctcct gcggggctcg acggggcgat
    17641 gagcaacctg acgagtgcgg tcaacctgct gttcggctgc tgcataggct tgcttgcggc
    17701 ggccgtggcg cggcggcttc acgacaccgg ccggaccggc ctgtggggat tgatgccggt
    17761 cccgttcatc ctcttttccg gcgcgatgat gccccgaatg ttcgccgagg acggacctcg
    17821 atcggacctc ttcttcgccg tcttcgtcag caactggctg tatctggccg cgctcgcctg
    17881 gctggccctg ctgctcgcca agcccggcga cccggcaggc aaccgctacg gaccaggcaa
    17941 gaccgcttag ccggctgcgg tcggtttggc gggcccggct ccggccccga gccgcgccaa
    18001 tgcggaacga ggccatttga ggttcgacgt gcaggcgggt gcgggccgga tcgacttcgg
    18061 gagcggcggc catgatcaag ccgtgcggct gtcgccggcc tgctgcgcct tgctgctcga
    18121 cgaccgcctg accggcttct ggttctcgct ccagcgttga acgccccccg ggccgaatgc
    18181 ccgcacgggt tcgcaagcgg attctagatc ggcaggtgcc ggctcacctt ggccttcagc
    18241 aggctgatca ggcttttgtc catgaactca tattcgtccg ggatgtcgag gcagatcacc
    18301 cgcgccttga ggtgctgcct gaaacgcttc tgaagccggt tgcggtgcgc tttctccatc
    18361 acgaagatca ggtcggccca ggctatcagc tccggggtcg tggggttttc ggcatcgttg
    18421 ttgagacccg ccgaggccac ttcgatgccc gggtgcgagg cgaacacctg ctccgccgta
    18481 gggctgcgga gcctgttctg gctgcacagg aacaggattt tcttcatcgg cgcaattggc
    18541 acggcgtgcc gaacgtccgc aatgcgtgga acgccggacc cgtcatcaac cgccactggg
    18601 cggcaggcgg tccatcagcc ttcccg
//
LOCUS       DASVRK010000303         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_496974, whole genome shotgun sequence.
ACCESSION   DASVRK010000303 DASVRK010000000
VERSION     DASVRK010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496974"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="VF589_12670"
     CDS             complement(<1..2285)
                     /locus_tag="VF589_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8258476.1"
                     /translation="MQLSDLSVKRPVFAAVLALLITIVGIVGFFSLSVREYPDVDPPI
                     VSIDTNYVGAAASVVETRVTQVLEEQLAGIEGLRTITSRSQDGSSDISIEFAPGRSVD
                     DAANDVRDRVGAVTDDLPDEVQPPEVRKVDADASPIMFLVISKPGWSRLELSDYVDRN
                     LVDRFSSIDGVARVFVGGEARPAMRVWMQPQRLAAFRLTPADVEQALRTQNVELPAGR
                     LESAQQNVTLRVERPFANAQQFAGLVVGRGEGGYLVRLSDIARIEQGPENPYSAFRLN
                     GEPAVGLGIVRQSGANTLAVADAAKAMAEQVKPNLPEGMEITVGSDESLFISRAIDKV
                     WVTLAEAAVLVVLVIYLFLGSWRATLIPAVTVPICLLASFAVLWMLGFSINLLTLLAL
                     VLSIGIVVDDAIVVLENVYHRIEEGEPPLAAAFNGTRQVGFAIVSTTLVVCAVFVPIM
                     FIAGQTGLLFRELAAAMIGAVAFSGFLALSLAPMLCSKLLRREERRGLAAFMDRGFRR
                     LEAFYARRLDGVLRRPLIPLAIVALFVAAGGGLFATLQSELVPAEDVGILSANVSAPE
                     GTGYAEMDRYMLEAQARMLPMLEGGAVRSIITRTPGGFGASDDFNSGSIIVFLKPWEE
                     RAATTADVVSEVNKELGQLAAVRGNASVRSSIGRGRGQPVNFVIAGATYEDLARARDR
                     IIAAAASNPGIINLDSDYKETKPQLRIDVDTNRAGDLGVSVEAVSQALQSLLGSRRVS
                     TFVDRGEEYRGVVQAEAGARAP"
     gene            complement(2290..>2926)
                     /locus_tag="VF589_12675"
     CDS             complement(2290..>2926)
                     /locus_tag="VF589_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8258477.1"
                     /translation="GARAEIGDRMITAPFSGWVSLRNISPGAVVQAGTEIATISDLSS
                     IKLDFQVPETMLPAIRTGQTIEAVSAAYPDQPFRGQIATIDPVIDPNTRSVMVRARLP
                     NPDRRLKPGMLLTVDIETAPRMSLSVPELAVIGEGDQRFVFTIAADGTAKRIPVRTGL
                     RSGGRIEIVEGLRPGQQVVTEGVVKVADGMKVRLAGAENAAPGPRPKAKGS"
BASE COUNT          475 a          955 c         1075 g          421 t
ORIGIN      
        1 ggtgcgcggg cgcccgcctc cgcctgcacg actccgcgat attcctcgcc ccggtcgacg
       61 aaggtggaga cgcggcgcga gccgagcagg ctctgcagcg cctggctgac cgcttcgacc
      121 gagacgccga gatcgccggc gcggttggtg tcgacgtcga tgcgcagctg cggcttggtt
      181 tccttatagt cgctgtcgag gttgatgatg cccggattgc tcgcggcggc ggcgatgatg
      241 cggtcgcggg cgcgggcgag atcctcgtaa gtggcgccgg cgatgacgaa attgaccggc
      301 tggccacggc cgcggccgat cgaggagcgc accgaggcgt tgccgcgcac ggccgcgagc
      361 tggccgagct ccttgttgac ctcgctcacc acgtcggcgg tggtcgccgc gcgctcctcc
      421 cacggcttta agaagacgat gatcgaaccg ctgttgaaat cgtcgctggc gccgaagccg
      481 cccggcgtgc gggtgatgat cgagcggacc gcgccgccct ccagcatcgg cagcatccgc
      541 gcctgcgcct cgagcatgta gcggtccatc tcggcatagc cggtgccttc gggggcggag
      601 acgttggcgg acaggatgcc gacatcctcg gccggcacca gctccgactg gagtgtggcg
      661 aagaggccgc cgcccgccgc gacgaacagg gcgacgatcg cgagcgggat caaaggtcgg
      721 cgcagcacgc cgtcgaggcg gcgggcgtaa aaggcttcga gccggcggaa gccgcggtcc
      781 atgaaggcgg cgaggccgcg ccgctcctcg cggcggagca gcttggagca gagcatcggc
      841 gcgaggctga gcgcgaggaa gccggagaaa gcgaccgcgc cgatcatcgc cgcggcgagc
      901 tcgcggaaca ggaggccggt ctggccggcg atgaacatga tcggcacgaa cacggcgcag
      961 acgaccagcg tggtcgagac gatcgcgaag cccacttggc gggtgccgtt gaaggcggcg
     1021 gcgagcggcg gctcgccctc ctcgatgcgg tgatagacgt tttcgagcac gacgatggcg
     1081 tcgtcgacga cgatgccgat cgacaggacg agcgcgagca gggtgaggag gttgatcgag
     1141 aagcccagca tccacagcac cgcgaagctg gcgagcaggc agatcggcac cgtcaccgcc
     1201 gggatcaggg tggcgcgcca gctccccagg aacaggtaaa tgacgagcac caccagcacc
     1261 gccgcctcgg cgagcgtcac ccagaccttg tcgatggcgc ggctgatgaa cagcgattcg
     1321 tctgagccga cggtgatctc catgccttcg ggaagattgg gcttcacctg ctcggccatc
     1381 gccttggcgg cgtcggcgac ggcgagcgtg ttggcccccg actggcggac gatgccgagg
     1441 ccgaccgccg gctccccgtt gagccggaag gcggaatagg ggttttccgg cccctgctcg
     1501 atgcgggcga tatcgctcag gcggacgaga tagccgccct cgccgcggcc gaccaccagg
     1561 cctgcaaatt gctgcgcgtt ggcgaacggc cgctcgaccc ggagggtgac gttctgctgg
     1621 gcggattcga ggcggccggc gggcagctcg acattctgcg tccgcagcgc ctgctcgacg
     1681 tcggcggggg tgaggcggaa ggccgcgagc cgctgcggct gcatccagac ccgcatcgcg
     1741 ggccgcgcct cgccgccgac gaacacgcgg gcgacgccgt cgatcgagga gaagcggtcg
     1801 acgaggttgc ggtcgacata gtcgctcaat tcaagccgcg accagccggg cttggagatg
     1861 acgaggaaca tgatcggcga ggcgtcggcg tcgaccttgc gcacctcggg cggctggacc
     1921 tcgtcgggga gatcgtcggt gaccgcgccg acgcggtcgc ggacgtcgtt ggcggcgtcg
     1981 tcgacggagc ggccgggcgc gaactcgatt gagatgtccg agctgccgtc ctgtgagcgc
     2041 gacgtgatcg tccgcaggcc ctcgatgccg gcgagctgct cctcgagcac ctgggtgacg
     2101 cgcgtctcga ccacgcttgc ggccgcgccg acataattcg tatcgatcga gacgatcggc
     2161 ggatcgacgt cgggatattc gcggaccgag aggctgaaga agccgacgat gccgacgatg
     2221 gtgatcaaaa gcgccagcac cgccgcgaag acggggcgtt tgacggagag gtcggagagc
     2281 tgcaccggct tagctgccct tcgccttggg acgcgggccg ggcgcggcat tctcggcgcc
     2341 ggcaaggcgg accttcatgc cgtcggcgac cttgacgacg ccctcggtta ccacttgctg
     2401 gcccggccgc agcccctcga cgatctcgat gcggccaccc gagcgcaggc cggtccggac
     2461 cggaatgcgc ttggcggtgc cgtcggccgc gatggtgaac acgaagcgct ggtcgccctc
     2521 gccgatcacg gcgagctcgg gcaccgacag cgacatgcgc ggcgcggtct cgatgtcgac
     2581 ggtgagcagc atacccggct tcagccgccg gtccggattg ggcagccgcg cccgcaccat
     2641 caccgaacgg gtgttgggat cgatcaccgg atcgatggtc gcgatctggc cgcggaacgg
     2701 ctggtccgga taagcggccg agacagcctc gatggtctgg ccggtgcgga tcgccggcag
     2761 catggtttcc ggcacctgga agtcgagctt gatcgagctc aagtcgctga tggtcgcgat
     2821 ttcggtgccc gcctgcacga cggcgccggg cgagatgttg cgcagcgaaa cccatcccga
     2881 aaagggcgcc gtgatcatgc ggtcgccgat ctccgcccgc gccccg
//
LOCUS       DASVRK010000304        26897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_555117, whole genome shotgun sequence.
ACCESSION   DASVRK010000304 DASVRK010000000
VERSION     DASVRK010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 26897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26897
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555117"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            546..1421
                     /locus_tag="VF589_12680"
     CDS             546..1421
                     /locus_tag="VF589_12680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8258478.1"
                     /translation="MEWFATLVAALVSLASALASPFGAGAGGETGSVTVPLEAAERGG
                     PRRDDPPILGSPDPSRPLVVIDAGHGGRDPGASSRHAARAEKHITLELARAIRDELVR
                     SGRLRVALTRDEDRYIDLRERYEIARRLGAELFVSVHADAAPHNDDARGATIYTLSEV
                     ASDKEAALLAASENRAGLIGGADLSEDPGVNRILIDLAQRESMERSAGFARLLHREAS
                     EIFPFRPEYHRFASLIVLKAPDIPSILFESGYLTNAEDAAYIQSSGGQTQIATGMRRA
                     IEAHFARRVVPVARS"
     gene            complement(1526..1909)
                     /locus_tag="VF589_12685"
     CDS             complement(1526..1909)
                     /locus_tag="VF589_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258479.1"
                     /translation="MGMLSWLLQLPFSRRQRIAMKLRGEVSLESFLNDISESPFEREV
                     ALSLWHHLRSQSVEPDFKPSPGDSLIHIYGIAEEELDEDLILSLLSKHGIPIPDPESI
                     TSFGPVSTPRDVVRFLAHLKRESRP"
     gene            complement(1912..2655)
                     /locus_tag="VF589_12690"
     CDS             complement(1912..2655)
                     /locus_tag="VF589_12690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX8258480.1"
                     /translation="MFTDFRSSAAAGAAQPSGGDPAAELYRPFARFVQPDPVGYDDGM
                     NLYAYTGNDPVNSTDPWGLSSMSDCQKDQAAGMAAGPEGAIVICRTRLINPPQESAVG
                     AFAGMGGGIGRGTEHSGGGEGAKKSDDSRLKKAGRCAADQLGLSELADAALVASGQPI
                     SGTKRFVTPGSSKGTSVAGLAASRVFGRAQLPVRMPTIVGGPGTGRTLSIAGTKSVAR
                     FAGRAVPILGWGLLAYDAISIAACTFSED"
     gene            complement(2645..3613)
                     /locus_tag="VF589_12695"
     CDS             complement(2645..3613)
                     /locus_tag="VF589_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258481.1"
                     /translation="MAGAFALRPEDEMPCAPAARQQSADPAAAVHAILSVPDDELDYA
                     CAKLALDRIVDPSIDVQATMVELHRMAGIARELAGPSATDGAKLAALRKLIYESGPWN
                     DHRPFGYDHSDPHGQHLPRKLLHNYLAGRLGQCVSMPILFLILADWMGLRVALASAPE
                     HIFVRYTDGLGRTINLETTSGAHPARDVWYRHCFLVNDRAIESGLYMRTLSRREGAAL
                     MATTVAEHLRAQGRYEEVIAVCETILEHDRRAGAVMVMQGSAYGVLLHQEFERQYPLP
                     FLIPEQLRARRLRLIERNNSLISAAEALGWSCGEFDSKEEGRSYVH"
     gene            3743..6289
                     /locus_tag="VF589_12700"
     CDS             3743..6289
                     /locus_tag="VF589_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX8258482.1"
                     /translation="MDTTTTRFTLYRENAGRFGSALRRLWARRWARWLALLIALPFIG
                     YLLVWAIFARGLPSAESLLTYQPPLPTNVRGIDGEPIQTFARERRVELDYAEYPRQLI
                     QAYLAAEDRTFFTHGGIDYPGIVGAIITNLKSGGRPVGASTITQQVAKNLLLTNEISY
                     TRKIKEAFLAGRIEDVLTKEQILELYLNQIFLGRNAYGVQSAARAYFDKDVEDLALHE
                     SAYLAILPKAPSNYSPERFPERAVGRRNWVLGEMERNGFITAAQRSQAQAEPLGTVRV
                     PMNTVKNVGGYFMEEVRRQLVQTYGENAEDSPNGVYQGGLWVRTSYDGIKQKAAETAL
                     RDALTRYDGGRGWRDPGVSIDMSREWRSQLAFAPFGAGYEDWRAAVVIDKTGSSATLG
                     FVDGTTGTLPRGNASMPKRGTATPAFDHLAPGKVIAVKQDGGSYYLRSIPEISGGMVV
                     QEVATGRVLAMQGGFDARLSDFNRATQAQRQPGSTFKPIVYSAALDNGMTPASIIVDG
                     SFCVFQSAALGSKCFRNFAGGSAGPQTMRWGIEQSRNLMTVRAANQIGMDKVIRLAKR
                     LNVGDYPNYLSISLGAGETTVMKMTNAFAIMANQGREVKPSLIDYIQDRNGKVIYRTD
                     NRCRVMQGCMARDWDGKPMPRPPLRTKQVMDPMTAYQMVHILEGVVIRGTAQRLRNLG
                     RPIFGKTGTTSGPNDVWFVGGTPDVVAGVYLGFDKPRSLGGAAQGGRLAAPVFEAFAR
                     ASFKDSPVVPFRAPAGIRMVRIDRRSGRKVFGAFPSSADPKSAVIWEAFKPESEPRRS
                     IRRDQIATKEDLLERLREIRARQAAQGRRSEPQEQQVQETDFLQREGGIY"
     gene            6400..7527
                     /gene="prfB"
                     /locus_tag="VF589_12705"
     CDS             6400..7527
                     /gene="prfB"
                     /locus_tag="VF589_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX8258483.1"
                     /translation="MRAEAQAYADQIRSAIDLLRRFLDWDRALRRLDELNARVEDPAL
                     WNDAKQAQAVMRERRRLDEAIGATRAISRELDDTMELIELAEAEGDEAMVDDAVAALA
                     ALAERAERDKVTALLAGEADANDTYIEVNSGAGGTESNDWAAMLQRMYQRWGERHGMK
                     VELIDQHSGEQAGIKSATMLLKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFASV
                     WVYPVVDDNIDIEINESELRIDTYRASGAGGQHINTTDSAVRITHLPTNIVVQCQNQR
                     SQHKNKAEAMNQLKARLYEAELQKREAEANATAAGKTDIGWGHQIRSYVLQPYQLVKD
                     LRTGITSTSPSDVLDGDLDRFMAAALSQKVTGEKVEVEDVE"
     gene            7520..8218
                     /locus_tag="VF589_12710"
     CDS             7520..8218
                     /locus_tag="VF589_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8258484.1"
                     /translation="MNRFRVSALPLLLLATTGCKAQPEASPFPEPQRPVAPIVSARYS
                     NEDSRDSVGEFDKVVELAGIGAGMSVADIGAGEGYYTIRLSPLVGAAGRVLAQDIVPA
                     TRDNLAQRVTRENLDNVAVKLGEPNDPKLPAGSFDRILLIHMYHEIERPSEFLWHLRD
                     GLKPDGRVVVVDADRSTDRHGTPPKLLMCEFASLGYELVRFERLPDSESYFAQFHGAG
                     PRPDPGKIPSCPAS"
     gene            complement(8421..8795)
                     /locus_tag="VF589_12715"
     CDS             complement(8421..8795)
                     /locus_tag="VF589_12715"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX8258485.1"
                     /translation="MLIGTAQAAADLLAPLFASAREERIVALHLDAGRRLLALTLEEP
                     GGAGDVELPVLLILQRALRIGSHAIILAHNHPSGDPEPSEADRMATAALADAARAVGL
                     RLDDHIVFGGSEWVSFRGLGLM"
     gene            8909..9703
                     /locus_tag="VF589_12720"
     CDS             8909..9703
                     /locus_tag="VF589_12720"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX8258486.1"
                     /translation="MQPYQQLLARILAEGVEQQDRTGVGTLSVFGHQMRFDLADGFPL
                     VTTKKLHLRSIVIELLWFLKGDTNVGWLKDNKVSIWNEWADENGDLGPVYGKQWRDWE
                     GPDGRRIDQIAALIEQINTNPASRRQIVSAWNPAEIDRMALAPCHCLFQTQVAAGKLN
                     LQLYQRSADVFLGVPFNIASYALLTHMLADQCGLEPGAFIWTGGDCHLYSNHLDQARL
                     QLSREPRPLPRLTIHRRAEAIDAYRYEDFEIGGYDPHPHIPAPVAV"
     gene            complement(9737..10912)
                     /locus_tag="VF589_12725"
     CDS             complement(9737..10912)
                     /locus_tag="VF589_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8258487.1"
                     /translation="MQPAAAALPAGAERVAAEGLFAIPGLIDVHQHLAIGQGRRAAEA
                     ALRREVHSGVTAVRDMTGDARILADLARSARLGEIPAADVYFAGLFAGPSFFTDERRA
                     ASAEGETVGKVPWLRAIRPEDDLRLAIAELRGTGASGIKVYGNLEPALVARIAAEARR
                     QGVPVWAHAAVFPATPLDVAAAAPATMSHVCMLGYQALKPPPPSYHDRAPLDEPSLGR
                     RRPHAEVAKALAAMRERGIVLDTTLWVYREIERMRREMKGYKPPTYCSSRLAEALAGQ
                     AYRAGVMLATGTDAPARAASAYPAVFDEMELLRGGAGLPPADMIRAATLNAARALGRD
                     GEMGAIEPGKLANIAFLAEDPLGPKAKLRSVVLTVKRGRPYWRRDYVPVTVEELNEF"
     gene            10817..11347
                     /locus_tag="VF589_12730"
     CDS             10817..11347
                     /locus_tag="VF589_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8258488.1"
                     /translation="MLVDVDQARNGEQPLGRDPLRAGGQRRRRRLHRRDPLAAQPRIE
                     GLLETALYVDDMARSRRFFAEVIGLAPMFESERLIAFDAGRQGVLLVFKRGASVEDMP
                     NPGGLVAGSDGSGPLHMAFAIAAESYDEWHSHLIASGVTMRGEMRWPRGGRSLYFEDP
                     DGHVLELATPGLWPNY"
     gene            complement(11348..12742)
                     /locus_tag="VF589_12735"
     CDS             complement(11348..12742)
                     /locus_tag="VF589_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823239.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX8258489.1"
                     /translation="MRVVTLAAEDDFEGWRDAARGLALAGEPPHRIAWRTGEGVGDLF
                     AMSGGEPPFEGSASAGFSVPRPFIELARSAICHSDPERFALLYTLLLRLKQEPGAMED
                     SADPLAGRLERMAKEVRRDIHKMHAFLRFREVTTEAGARYVAWFEPDHHIVRAAAGFF
                     VRRFANMPWSILTPALSLHWDGATLTEAPGATRADAPDGDPVEETWKTYYASIFNPAR
                     LKVGAMTKEMPKKYWKNMPETALVGGLIAGAQAREAQMVESSRSGIGGNVRAAWEAIR
                     DEALGCTRCPLYKTGTQTVFGEGPLDARIMFVGEQPGDQVDLAGKPFVGPAGQLLDRA
                     LGDAGVDRGAAYVINAVKHFKFEARGKRRIHSKPNGAEIDACRWWIEQERLLIRPPIT
                     VALGATAARSLFGKAVTIGSMRGRPHPIYDGGEGWVTVHPSFLLRVRDNREAEYARFV
                     EDLRLIGQRAEALA"
     gene            12823..14145
                     /locus_tag="VF589_12740"
     CDS             12823..14145
                     /locus_tag="VF589_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8258490.1"
                     /translation="MTEGPQPTLGARELAAVTVGIVIGAGIFRTPSLVAGASGSEAVF
                     LMTWAAGGLLSIVGALCYAELASAFPSAGGDYHFLARAFGRRLGFLYAWARLAVIQTG
                     SLALLAFVFGDYMSALAPLGSASPALYAAAAVIGLTALNWAHVRGGTGSQLWLTAIEV
                     GGLIALIGIGLGMAGMAAPSEAPSPEAGSIGLTLVFVLLTFGGWSEAAYLSAELRGGR
                     RRIATVLVGSLAFVTLLYLLVNWAYLRALGLGGIAASDAVAADMVRPVAGAAGVAAIS
                     LLVAIAALTSANATAITGARTTYALGRSFPRLRWLGRWHAASGTPRNALLFQGAVALL
                     LIGAGAFARDGFQLVVEYTAPVFWLFLVLVGISLFVLRAREPNAERPFRVPLYPWLPI
                     VFCMTSAFLFYSSLAYTGASALVGVAVLAAGGLLLLFLEPDADEEMSP"
     gene            14142..14720
                     /locus_tag="VF589_12745"
     CDS             14142..14720
                     /locus_tag="VF589_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8258491.1"
                     /translation="MKILLAMLALGTAAAAGAVHVSGAGQAEPSGTAAPAAQREPDVI
                     YVPTPPPVVQAMLRLGGVKKGDLLYDLGSGDGRIPIAAAKVYGVRGVGIDIDPKRIAE
                     ANANAKAAGVAHLVSFRQADLFETDFHEASVVTLYLLPSLNEKLRPRLLAQLKPGTRI
                     VSHAFDMGEWKPEVTQQVEGSTIYLWTVPPRR"
     gene            complement(14732..15976)
                     /locus_tag="VF589_12750"
     CDS             complement(14732..15976)
                     /locus_tag="VF589_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HEX8258492.1"
                     /translation="MPQLDVQAKLAILADAAKYDASCASSGTAKRSSAGGKGIGSTEG
                     MGICHAYAPDGRCISLLKILLTNSCIFDCHYCINRKSSNVRRARFTAEEVVRLTLAFY
                     KRNYIEGLFLSSGIIRSSNYTMEQIVEVARSLREDHDFHGYIHLKTIPDADPELIRQA
                     GLHADRVSINVELPTARGLERLAPDKDGARIEAAMKDMKTAIDDGADAARRFKSAPAF
                     APAGQSTQMIVGADSASDGEIIRRASQLYDHFALRRVYYSAFSPIPDASAVLPLQRPP
                     LMREHRLYQSDWLMRFYGYAPAEVAAAADPAGMLPLDIDPKLAWALKHREHFPVDVNR
                     APRERLLRVPGLGVKAVDKILTSRRWRRLTLDDVARLTVSVTKVRPFLVAEGWRPTLL
                     TDRADLARRLRPNVEQLEMFAA"
     gene            16282..16689
                     /locus_tag="VF589_12755"
     CDS             16282..16689
                     /locus_tag="VF589_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8258493.1"
                     /translation="MVTLLLPACATVTRGTKQTYVIESSPPNAQVALSTGQKCVTPCR
                     LKLKRKYDFTATFTKPGYDPATAQVRSKLSTGGGVAAAGNIIAGGIIGGIVDGTNGST
                     NDLTPNPLVVTLKPNGQPTAAAPAAGSPTGGNR"
     gene            complement(16762..17640)
                     /locus_tag="VF589_12760"
     CDS             complement(16762..17640)
                     /locus_tag="VF589_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8258494.1"
                     /translation="MALPALVAAASSLLALTASTTALSKPGLSPNGLSIELAPASLLV
                     ANLEGLLNDEVLSVDGDLIHVGALQPARPFRIVQAGSATQRRSLDCLAAAVYHEARSE
                     GEAGQRAVAQVVLNRVRHPSFPATVCGVVFQGAGRRTGCQFTFTCDGSLRRRRVPAAW
                     EEALQIAEEALAGRVYAPVGTATHYHTNQVNPYWAGTLTRITELGAHIFYRWRGAPGE
                     LNAFTRYPAPEPAVALAAAEPSRGRTETRLAAAVTAPAAPVEKALPAPLDAPSMLSAT
                     GFSWRGDSRMSIGPAS"
     gene            complement(17779..19152)
                     /locus_tag="VF589_12765"
     CDS             complement(17779..19152)
                     /locus_tag="VF589_12765"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX8258495.1"
                     /translation="MTTRTASDDADPRVESIEDLLAPFARGEKPPERWRIGTEHEKFV
                     YRRSDHRAPSYEEPGGIRDLLSELTQYGWQPVVENGNVIALSGADGNVSLEPAGQLEL
                     SGAPLEHLHQTCAEAGRHLQQVKAVGEKLGLGFLGLGMWPDKTRDALPRMPKGRYAIM
                     LNHMPKVGTLGLDMMLRTCTIQVNLDYASEADMAQKFRVGLALQPLATALFANSPFTE
                     RRPNGFLSYRSHIWSDTDGARTGMLPFVFESGFGYERYRDYALDVPMYFVYRGGRYVD
                     AAGQSFRDFLKGELPALPGEKPLLSDWNDHLSTAFPEVRLKSFLEMRGADGGPWNRIC
                     ALPALWVGLLYDGAALDAAWDEVRHWTLEERQQLRDAVPKEGLAARTPAGETLQQLGQ
                     RILAIAEAGLNARARLNASGDNESGFLDPLREIVARGATPADRLLASFHGEWEGDVSR
                     VYEEMSF"
     gene            complement(19203..19952)
                     /locus_tag="VF589_12770"
     CDS             complement(19203..19952)
                     /locus_tag="VF589_12770"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX8258496.1"
                     /translation="MPATPAWPPHSLPRLYVDQPLSEGAALSLDGAPANYLGNVLRLA
                     AGAQVKLFDDRTGEWLAEISEAGKRRVALTILSRLREREAVPDLWLLLAPVKRGRIDW
                     LVEKATELGAARLVPVLTRRTIVDRLNLDRLRAHMIEAAEQCERTALPELAEPQKLPA
                     LLAAWPEDRALIFADEQGGEPLGPGAGPAAILIGPEGGFTDEERAAIRALPQALPTSL
                     GPRILRADTAALAAISLWMAMSGDWTGDSTR"
     gene            20082..21002
                     /gene="ubiA"
                     /locus_tag="VF589_12775"
     CDS             20082..21002
                     /gene="ubiA"
                     /locus_tag="VF589_12775"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX8258497.1"
                     /translation="MGIEAGETDIVPDSERRGLIGALPRAVRPYASLMRLDRPIGAWL
                     LFWPGAWSVALAGMGSRGVALILWLGLGAFAMRSAGCVYNDIIDRELDRQVARTRLRP
                     LASGRVPLKGAWALLVGLSLIGLIVLLQIGPLAQLVALASLALVAAYPFMKRITWWPQ
                     AWLGLVFSWAALVGWPAVEGEIDAAALWLYAGSVAWVIGYDTIYALQDREDDALVGVK
                     SSALALGERARAGVAIFYLIALAAWGAAIWQVRADWLGLAALLPMALHLMWQVATLRQ
                     EDGADALAKFRSNRFAGFIMFLASLVVGTT"
     gene            21035..22381
                     /locus_tag="VF589_12780"
     CDS             21035..22381
                     /locus_tag="VF589_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX8258498.1"
                     /translation="MLTAKEAEARAGALIEAAMKAGADAADVLYVGDGSTGVQVRLGE
                     LEDVERSEGEEIGLRFFVGKRSAGVASSDLSREALAALVERAAAMAREAPEDEYAGLA
                     PEHRLLRDGGPDIAGDDGRDPAPAELKAMSLAIEAAALAVPGVTNSQGAGVSAGRSVV
                     ALATSHGFCRGYTTSGYSGSASVIAGSGANMQRDYASHGVRHLEDLDDPETIGRLAGE
                     RAVARMDPGKLPSGPMPVVFDPRVGGSLVNHLLGAITGSAIARKTSFLLGREGELVFP
                     EGVTIRDDPHRRRGLRSKAFDGEGMATRATDLVDRGRLTGWLLDSASARQLGLEPTGH
                     STRGVGGSPGASATNVEMLPGTLSKAELIADIGRGLYVTELIGQGVNPVTGDYSRGAS
                     GFIIEQGEITVPVAEITIAGNLVDMFRELVPASDLEHRRAVDVPTIRIDGMTIAGA"
     gene            22374..23138
                     /locus_tag="VF589_12785"
     CDS             22374..23138
                     /locus_tag="VF589_12785"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX8258499.1"
                     /translation="MLDAVAKIAAEAGRIAHAKSGSAFRQWEKVPGHPVCDIDIEIDA
                     FLREQLAALDPEAGWLSEETLDASDRYERRRAWVVDPIDGTRDYLRGRSGWCVSVALV
                     EDRVPLIGVLDAPARGEVWTAERGRGAWRNGARLRVSERRELAGARVPTERLATPDAD
                     LTPVAKPNSIALRIAMVAAGEADLVATLRWGFEWDIAAAALIASEAGATVTGALGQPF
                     AFNTHSGEAFGVLAATPGIHAAAAARLRERAEAAVG"
     gene            complement(23212..23640)
                     /locus_tag="VF589_12790"
     CDS             complement(23212..23640)
                     /locus_tag="VF589_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX8258500.1"
                     /translation="MPLTADRDIAELLAETRTIALIGASDRPGRPSYGVMRFLQDHGY
                     RVLPVNPQITGEHVHGEYVWRELSQIGEPIDMVDIFRRPIAAGEAVDEAIAAGAKSVW
                     LQIGVINEEAAARAEAAGLKVVMDRCPAIEIPRLRVPRIG"
     gene            complement(23676..24644)
                     /locus_tag="VF589_12795"
     CDS             complement(23676..24644)
                     /locus_tag="VF589_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX8258501.1"
                     /translation="MRALARVPDPAGGDLADSGRVSAPRLQGGVASMIVDATGLGAEA
                     RAELERAIRASLAAEPGIDEIRIAMTADKVERKLIAVGSGKGGVGKSTLAANLAVALA
                     RTGRKVGLVDADIYGPSQPRLMGSHDKPEAVGKQIVPTLAHGVRMLSIGQLVEAGIAL
                     AWRGPMVAGALGQLLDGDWGDTELMIVDLPPGTGDIQLSLIQKYRPAGAVIVSTPQDL
                     ALIDATRAIDLFRKMHVPILGLVENMAGYACPHCGEVSDPFGSGGAEAAARVMAIPFL
                     GRVPLTLAIRRASDEGVPPAAGDAEEAGYYRAIAARLIEEMNRTPA"
     gene            24741..25862
                     /gene="hflK"
                     /locus_tag="VF589_12800"
     CDS             24741..25862
                     /gene="hflK"
                     /locus_tag="VF589_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX8258502.1"
                     /translation="MRRIFGWGARPGAVFNENRGGPWGTGGGGGGDGDGPRNPWGQPP
                     RKRRPGGGAGNVTSLDEFLKKSRQRIGSGLPQGDGRPYWLYGLAIFVLLWLLFTSIHR
                     IGPQERGVVTRLGSYSGTMEPGIAFSLPAPIDTVRKVDVEEIRTIDIGSTDASSENLI
                     LTADQNIIDLAYSVRWNISDPELYLFQIANPEETIREVAESAMRAVVAGVSLDDAIGA
                     GRGEIEQRVQTVMQQLLNDYRAGVRVQGVAVKQSDPPAAVNDAFKEVSAAQQQAQSYM
                     NDARAYALQLTAKAQGEAAAFDKVFTQYKLSPEVTRRRMYYETMEKVLSRVDKTIIEA
                     PGVTPYLALPEVQRRSQSQVQAQPQPQPQPQPQQGGTGQ"
     gene            25862..26725
                     /locus_tag="VF589_12805"
     CDS             25862..26725
                     /locus_tag="VF589_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX8258503.1"
                     /translation="MQRLGRNPIALGVLAVLLLILVFSTFSVVPETRQAVIVRFGEPD
                     RIINSYRPNTPFGQTGAGLIARIPFMEEIHWIDKRILSVEMDQQQVLSTDQLRLQVDA
                     FARYRIVDPLRMFISARTEERVSDQLRPILGSALRNELGKRPFAALLSPERGQMMDNI
                     QRALNRVARQYGAEIVDVRIKRADLPEGTPLTSAYQRMETARQQEARSIRAQGLKQAQ
                     IIRAEADAEAARTYAEAFNKDPDFYDFYRAMQSYEASFVDNGAGQETMGRSSIVLSPS
                     NDYLREFRGRR"
BASE COUNT         4211 a         9209 c         9280 g         4197 t
ORIGIN      
        1 aaattcctcg atccggtttc ccttgatcac ggccacgcgg gtttcttcct ggtggcgcgc
       61 gtccactaac atgcgcatcg tcattgatta tactccacgc gcgcggcgcc gtggccaagc
      121 cgtacaggct cggggccggt cgcgccgcgc gttcgtaaaa ggacgaaggc gcggccccgg
      181 gacctgacgg tctgtcggcg cgcgcctcga atgtccgggt tgtacagaaa agtgcctctg
      241 ctgcctgcgg caggccggtc tgtggtgccg gccgaaacgt ccccgccacg ccatgacccg
      301 aaagggggcg ctcagattgc ggggaaaaat gcctcatgca gcgtcaacct aggtggccgg
      361 cgtcaggagc cggcgtaact cgctcggctc gacaggctga cggagcttcc gttatctctc
      421 gaaccgatgc cggactgcta gcaccggcaa gccgaagcgg caaccgctaa ggcggcgaaa
      481 taccgcgctt tggcggcatg gcttttcgct ggaccgtgcg gcccggccca aggcagatag
      541 gggttgtgga atggtttgca actctggttg ccgcgttggt cagcctcgcc tccgcgctgg
      601 cctcgccgtt cggcgccggg gcgggcggcg agacgggcag cgtcaccgtg ccgctcgaag
      661 cggccgagcg cggcggcccg cgccgcgacg atccgccgat cctaggctcg cccgatccga
      721 gccggccgct ggtggtgatc gacgccggcc atggcggccg cgatcccggc gcctcctcgc
      781 gccacgccgc gcgcgccgaa aagcatatca cgctggagct ggcgcgcgcg atccgcgacg
      841 agctggttcg ctcgggccgg ctcagggtcg cgctcacccg cgacgaggac cgctatatcg
      901 accttcgcga acgctacgag atcgcccgcc ggctcggcgc cgaactgttc gtctcggtcc
      961 atgccgacgc cgcgccgcat aacgacgatg cccgcggcgc caccatctac accttgtccg
     1021 aagtcgcctc cgacaaggag gccgctttgc tcgccgccag cgagaaccgg gccgggctga
     1081 tcggcggcgc cgacctcagc gaggatccgg gcgtcaaccg catcctcatc gacctcgccc
     1141 agcgcgagag catggagcgc tcggccggct tcgcccgcct gctccaccgc gaggcgtcgg
     1201 aaatcttccc gttccgcccc gaatatcacc gcttcgcctc gctgatcgtg ctgaaggcgc
     1261 ccgacattcc ctcgatcctg ttcgaatccg gctatctcac caacgccgag gacgccgcct
     1321 acatccagtc gtcgggcggc cagacccaga tcgccaccgg catgcgccgc gcgatcgaag
     1381 cccatttcgc ccggcgcgtg gtgccggtgg cgcggagcta aggtcgcggg gcggaagtgc
     1441 gcggccgcac aggacctcgc catgctcagc gccgaccagc gagcgtgcga tgcgggaaac
     1501 ggactttgcc agctgtgacc gttacctacg gccgcgactc ccgtttaaga tgggccagaa
     1561 atctgacaac atctctcggg gtggaaaccg gtccgaaaga ggtaattgat tcaggatcag
     1621 gtatgggaat cccgtgcttt gaaagaagcg aaagaatcaa gtcttcgtcc agctcctcct
     1681 ccgcgatgcc atatatgtgg atcaacgaat cgccgggcga aggcttgaaa tcaggctcta
     1741 cggattggga cctgaggtga tgccacaagc taagcgccac ctctcgttca aacgggcttt
     1801 cggagatgtc gttcaggaag ctttcgaggc tgacttcccc ccggagcttc atcgcaatcc
     1861 tctgccggcg ggagaacggc aactgaagca gccagctcag catccccatt gtcaatcctc
     1921 gctgaaagta catgccgcta tgcttatcgc atcataagcc aaaagccccc agccaagaat
     1981 gggaacggcg cgaccggcaa aacgggctac agacttggtc cctgcgatcg ataaggttcg
     2041 gcccgtcccg ggacctccta cgatcgtcgg catgcgcacg ggcagctgag cgcgtccaaa
     2101 gactcggcta gcagccaggc cggccactga cgtccctttg gagctgccgg gtgtgacaaa
     2161 ccgctttgtc cccgaaatag gttggccact cgcgacgaga gctgcgtcag cgagttcgga
     2221 caaacccagc tggtccgccg cacagcgacc ggccttcttc agccggctgt catcgctttt
     2281 cttcgctcct tcaccgcctc cgctgtgctc ggttccacgt ccgatgccgc cccccatgcc
     2341 ggcaaatgct ccgacggcgc tctcctgggg cggattgatc aaccgtgtgc ggcaaataac
     2401 gatggcgccc tcgggtcccg ccgccatccc ggccgcctga tctttctggc agtcgctcat
     2461 gctggacagg ccccaggggt ccgtggaatt gaccggatcg ttcccggtat aagcatagag
     2521 gttcatcccg tcgtcatagc cgaccgggtc gggctgcacg aaacgtgcga agggccgata
     2581 cagctcggcg gccgggtccc ccccactcgg ctgcgccgcg cccgctgcgg ccgacgatct
     2641 gaaatcagtg aacatagctt ctgccttcct cttttgaatc aaattcgcca cagctccagc
     2701 cgagcgcctc cgccgcgctg atgagggagt tgttgcgctc gatcagtcgc aaccggcgcg
     2761 cccgcaactg ttccgggatg aggaacggca gcgggtattg ccgctcgaac tcctggtgca
     2821 ggaggacccc gtaggcggag ccctgcatca ccatgactgc accggcccgg cggtcgtgct
     2881 cgaggatagt ctcacagact gcgatcacct cctcatagcg cccttgcgcc ctcagatgct
     2941 cggcaacggt cgtggccatc agggcggcgc cctcacggcg gctcagggtc cgcatgtaga
     3001 gaccgctctc tatggcgcgg tcattcacca ggaagcagtg gcggtaccag acatccctcg
     3061 caggatgcgc cccactggtc gtttcgagat ttatcgtgcg cccgagaccg tcggtataac
     3121 gaacgaagat atgctcgggc gcgctggcga gcgccacgcg gagccccatc cagtcggcca
     3181 aaatcaggaa caggatcggc atcgacacgc attggccgag ccggcccgca aggtaattgt
     3241 gcaggagctt tcgcgggaga tgctgtccgt gcgggtcgga atggtcatag ccaaacggcc
     3301 gatgatcgtt ccacggtccg ctttcgtaga tcagcttccg cagcgcggcg agcttcgcgc
     3361 cgtccgttgc cgacgggccc gccagctccc tggcgatacc ggccatgcgg tggagctcca
     3421 ccatcgtcgc ctgcacgtcg atcgacggat ccacgatccg atcgagcgcc agcttcgcac
     3481 acgcatagtc gagctcgtcg tcgggcaccg aaagtatcgc gtggactgcc gccgccggat
     3541 cagccgactg ctgccgcgcc gcgggggcac acggcatttc gtcttccggg cgtagggcga
     3601 acgctcctgc catcttcaac tcttcctcgg ggccgactcg tgccgcgcgt tggaacagaa
     3661 aaagaacacc tgaaatccta cattgcaagt ccttgccgag acggcttcac ccgccgcgac
     3721 gcatcggcta aaccggcctc aaatggatac caccaccacc cgcttcaccc tgtatcgcga
     3781 aaatgccggc cggttcggct cggcgctgcg gcgcctgtgg gcgcggcgct gggcgcgctg
     3841 gctcgcattg ctgatcgcgc tgccgttcat cggctatctg ctcgtttggg cgatcttcgc
     3901 gcgcgggctg ccgtcggccg aatccctgct cacctaccag ccgccgctgc cgaccaacgt
     3961 gcgcggcatc gacggcgagc cgatccagac cttcgcccgc gagcgccgcg tcgagctcga
     4021 ctacgccgaa tatccgcgcc agctgatcca ggcctatctc gccgccgagg accgcacctt
     4081 cttcacccat ggcggcatcg attatcccgg catcgtcggg gccatcatca ccaatctgaa
     4141 gagcggcggc cgtccggtcg gcgcctcgac catcacccag caggtcgcca agaatttgct
     4201 gctcaccaac gagatcagct acacccgcaa gatcaaggaa gccttcctgg ccggccgcat
     4261 cgaggacgtg ctgaccaagg agcagatcct ggagctctac ctcaaccaga tcttcctcgg
     4321 ccgcaacgcc tatggcgtcc agtcggccgc gcgcgcctat ttcgacaagg acgtcgagga
     4381 cttggcgctc cacgaatccg cctatctcgc catcctgccc aaggcgccct ccaattacag
     4441 ccccgagcgc ttccccgagc gcgcggtcgg gcggcgcaac tgggtgctcg gcgagatgga
     4501 gcgcaacggc ttcatcaccg ccgcccagcg cagccaggcc caggccgagc cgctcggcac
     4561 cgtccgcgtg ccgatgaaca cggtcaagaa tgtcggcggc tatttcatgg aggaggtgcg
     4621 ccgccagctc gtccagacct atggcgagaa tgccgaggac agcccgaacg gcgtctacca
     4681 aggcgggctg tgggtccgca cctcctatga cggcatcaag cagaaggcgg ccgagaccgc
     4741 gcttcgcgac gcgctcaccc gctatgacgg cggccgcggc tggcgcgatc ccggcgtcag
     4801 catcgacatg tcgcgcgaat ggcgcagcca gctcgccttc gctccgttcg gcgccggcta
     4861 tgaggattgg cgcgcggcgg tggtgatcga caagaccggc agcagcgcca cattgggctt
     4921 cgtcgacggc accaccggca cgctgccgcg cggcaacgcc tcgatgccca agcgcggcac
     4981 cgccaccccc gccttcgacc atctcgcgcc cggcaaggtg atcgcggtca agcaggacgg
     5041 cggcagctat tatcttcgct cgatccccga aatctccggc ggcatggtcg tccaggaagt
     5101 cgccaccggc cgcgtcctcg ccatgcaggg cggcttcgac gcgcgccttt ccgacttcaa
     5161 ccgcgctacc caggcccagc gccagcccgg ctccaccttc aagccgatcg tctattcggc
     5221 cgcgctcgac aacggcatga cgcccgcctc gatcatcgtc gacggttcgt tctgcgtgtt
     5281 ccagtcggcc gccttgggct ccaaatgctt ccgcaacttc gccggcggca gcgccggtcc
     5341 gcagaccatg cggtggggca tcgagcagtc gcgcaacctg atgacggtcc gcgccgccaa
     5401 ccagatcggc atggacaagg tgatccggct cgccaagcgg ctgaacgtcg gcgactatcc
     5461 caattacctg tcgatctcgc tcggtgccgg cgagacgacg gtgatgaaga tgaccaacgc
     5521 cttcgcgatc atggccaacc agggccgcga ggtgaagccg agcctgatcg actatatcca
     5581 ggaccgcaac ggcaaggtca tctaccgcac cgacaatcgc tgccgcgtca tgcagggctg
     5641 catggcgcgc gattgggacg gcaagccgat gccgcgcccg ccgctgcgca ccaagcaggt
     5701 gatggacccg atgaccgcct atcagatggt ccacatcctc gaaggcgtgg tgatccgcgg
     5761 caccgcccag cggctgcgca accttggccg cccgatcttc ggcaagaccg gcaccacgtc
     5821 ggggcccaac gacgtctggt tcgtcggcgg cacgcccgac gtcgtcgccg gcgtctatct
     5881 cggcttcgac aagccgcgca gcctcggcgg cgccgcgcag ggcggccggc tcgccgcgcc
     5941 ggtgttcgaa gccttcgccc gcgcctcgtt caaggattcg ccggtggtgc cgttccgcgc
     6001 ccccgccggc atccgcatgg tccgcatcga ccggcgctcg ggccgcaagg tgttcggcgc
     6061 cttcccgtcc tcggccgacc ccaagtcggc ggtgatctgg gaggcgttca agccggagag
     6121 cgagccgcgc cgctcgatcc gccgcgacca gatcgcgacc aaggaggatc tgctcgagcg
     6181 gctgcgcgag atccgcgccc gccaggccgc ccagggccgc cgctccgaac cgcaggagca
     6241 gcaggtccag gaaaccgact tcttgcaaag agagggcggc atttactagg tctgcggcgc
     6301 ggcgcgccgc aaaccgtatc cctgcgcagg cagggatcca gccgaagaag attctggacc
     6361 ccggctttcg ccggggaaca gtccaccgaa ggacctccga tgcgcgccga agcgcaagcc
     6421 tatgccgacc agatcaggtc cgcgatcgat ctcctccgcc ggttcctcga ttgggaccgc
     6481 gcgctgaggc ggctcgacga gctcaacgcc cgggtcgagg acccggcctt gtggaacgac
     6541 gccaagcagg cccaggcggt gatgcgcgag cgccgccggc tcgacgaggc gatcggcgcc
     6601 acccgcgcga tctcccgcga gctcgacgac accatggagc tgatcgaact cgccgaggcc
     6661 gagggcgacg aggcgatggt cgacgacgcc gtcgccgcat tggcggcgct ggccgagcgc
     6721 gccgagcgcg acaaggtcac cgctttgctg gccggcgagg ccgacgccaa cgacacctat
     6781 atcgaggtca attcgggcgc cggcggcacc gagagcaacg attgggcggc gatgctccag
     6841 cgcatgtacc agcgctgggg cgagcgccac ggcatgaagg tcgagctcat cgaccagcat
     6901 tcgggcgagc aggccggcat caagtccgcg accatgctct tgaagggcga gaacgcctat
     6961 ggctatgcca agaccgagag cggcgtccat cgcctggtgc ggatcagccc ctatgacagc
     7021 tcggcccgcc gccacaccag cttcgcctcg gtctgggtct atccggtagt cgacgacaat
     7081 atcgacatcg agatcaacga aagcgagctt cgcatcgaca cctaccgggc cagcggcgcc
     7141 ggcggccagc atatcaacac gaccgattcg gcggtccgca tcacccattt gccgaccaac
     7201 atcgtcgtcc agtgccagaa ccagcgctcg cagcacaaga acaaggccga ggcgatgaac
     7261 cagctcaagg cccggctcta cgaggccgag ctgcaaaagc gcgaggccga ggccaacgcc
     7321 accgccgccg gcaagaccga catcggctgg ggtcaccaga tccgttccta cgtgctccag
     7381 ccctatcagc tggtgaagga cctgcgcacc ggcatcacct cgacctcgcc gtcggacgtg
     7441 ctcgatggcg acctcgaccg cttcatggcc gcggccttgt cgcagaaagt gaccggcgag
     7501 aaggtcgaag tcgaagatgt tgaataggtt tcgcgtctcc gcgctccctt tgctcctgct
     7561 cgccaccacc ggctgcaagg cgcagcccga agcctcgccc ttccccgagc cgcagcggcc
     7621 ggtcgcgccg atcgtctcgg cccgctattc gaacgaggat tcccgcgaca gcgtcggcga
     7681 gttcgacaag gtcgtcgagc tggccggcat cggcgcgggc atgtcggtcg ccgatatcgg
     7741 cgccggcgag ggctattata ccatccggct gtcgccgctg gtcggagccg ccggccgggt
     7801 cctcgcccag gatatcgtgc cggcgacgcg cgacaatctc gcccagcgcg tcacccgcga
     7861 gaatctcgac aatgtcgcgg tcaagctcgg cgagcccaac gatcccaagc tccccgccgg
     7921 ctcgttcgac cgcatcctcc tcatccacat gtatcacgag atcgagcggc cgagcgagtt
     7981 cctctggcac ctgcgcgacg gtttgaagcc cgacggccgc gtcgtcgtgg tcgacgccga
     8041 ccgctcgacc gaccgccacg gcaccccgcc caagctgctg atgtgcgagt tcgcctcgct
     8101 gggctacgag ctggtgcggt tcgagcgcct gcccgattcc gaatcctatt tcgcccaatt
     8161 ccacggcgcg ggcccccgcc ccgatcccgg caaaatcccg agctgccccg cttcgtaggg
     8221 gtgggtccgc agggaacggg tctttggagc agcatgctta cccgctcatc ctgaggaggg
     8281 actgagcctt ggcgaaggcc cgtctcgaag gacgcacggt ggtccttcga gacgccgctt
     8341 cgacttcgct cagcggctcc tcaggatgag cgatgatcct tgaaccccct cccgttcccc
     8401 ggcgaaggcc ggggcccagc tcacatcagc cccagccccc ggaagctcac ccactcgctc
     8461 ccgccgaaca cgatatggtc gtcgagccgc agcccgaccg cgcgggcggc gtcggccagc
     8521 gccgccgtcg ccatccgatc cgcctcgctc ggctcgggat cgccgctcgg atgattgtgg
     8581 gcgaggatga tcgcgtgcga gccgatcctg agcgcccgct gaaggatcag cagcaccgga
     8641 agctcgacgt cgcccgcccc acccggctcc tcgagcgtca gcgccagcag ccgccgcccg
     8701 gcatcgagat ggagcgcgac gatgcgctcc tcccgcgccg aggcgaagag cggcgcgagc
     8761 aggtccgccg cggcctgggc cgtgccgatg agcatcgtcg cggcttgcca gcttttgccg
     8821 cgccgggccg gggaagcttg gtccgaaaaa tatccgaaat ggaggaagcc ccgttggaaa
     8881 gccgcgccgc cccggccata ggtgaggcat gcagccttat cagcaactcc tcgcccgcat
     8941 cctcgccgaa ggcgtcgaac agcaggaccg caccggcgtc ggcaccctgt cggtgttcgg
     9001 ccaccagatg cgcttcgacc tggccgacgg ctttccgctg gtcaccacca agaagctcca
     9061 cctgcgctcg atcgtcatcg agctgctctg gttcctcaag ggcgacacca atgtcggctg
     9121 gctcaaggac aataaggtca gcatctggaa cgaatgggcg gacgagaatg gcgacctcgg
     9181 cccggtctac ggcaaacagt ggcgcgactg ggaggggccg gacggccgcc gcatcgacca
     9241 gatcgccgcg ctcatcgagc agatcaacac caaccccgcc tcgcggcgcc agatcgtctc
     9301 cgcctggaac ccggccgaga tcgaccgcat ggcgctcgcc ccctgccatt gcctgttcca
     9361 gacccaggtc gcggccggca agctgaacct ccagctctac cagcgcagcg ccgacgtctt
     9421 cctaggcgta ccgttcaaca tcgccagcta cgcgctcctc acccatatgc tggccgatca
     9481 atgcgggctc gagcccggcg ccttcatctg gaccggcggc gactgccacc tttactccaa
     9541 ccatctcgac caggcccgcc tccagctcag ccgcgagccg cgcccgctgc caaggctcac
     9601 catccaccgc cgcgccgaag cgatcgacgc ctatcgctac gaggatttcg agatcggcgg
     9661 ctacgatccc cacccccaca tcccggcgcc ggtggcggtg tagccgaagg ccgtagcctc
     9721 ggccggtcag ctgccactaa aactcgttca gctcctcgac ggtgacaggc acatagtcgc
     9781 gccgccaata gggccggccg cgcttcaccg tcagcaccac cgagcgcagc ttcgccttcg
     9841 gccccagcgg gtcctcggca aggaaggcga tattggcaag cttgccaggc tcgatcgcgc
     9901 ccatctcgcc gtcccgtccg agtgcccgcg cggcattgag cgtggcggcg cggatcatgt
     9961 ccgccggcgg caggccggcg ccgccgcgca gcaactccat ctcgtcgaac acggccggat
    10021 aggcgctcgc cgccctggcc ggcgcgtcgg tccccgtcgc cagcatcacc ccggcccgat
    10081 aggcctgccc cgccagcgcc tcggccagcc ggctgctgca ataagtgggc ggcttgtagc
    10141 ccttcatctc gcgccgcatc cgctcgatct cgcgatagac ccacaaggtc gtgtcgagca
    10201 cgatgccgcg ctcgcgcatc gccgccagcg cctttgccac ctcggcatgc ggccgccgcc
    10261 ggccgagcga cggctcgtcg agcggcgcgc ggtcgtgata gctgggcggc ggcggcttca
    10321 gcgcctgata gcccagcata cagacgtgcg acatggtcgc gggcgccgcc gcggcgacgt
    10381 ccagcggcgt cgccgggaac accgccgcat gcgcccagac cggcacaccc tgccgccgcg
    10441 cctcggccgc gattcgcgcc accagcgccg gctcgaggtt gccgtagacc ttgatccccg
    10501 acgcgccggt gccgcgcagc tcggcgatgg cgagccgcag atcgtcctcc gggcggatcg
    10561 cccgcagcca cggcaccttg ccgaccgtct cgccctcggc cgaggcggcc ctgcgctcgt
    10621 ccgtgaagaa gcttggcccg gcgaacaggc cggcgaaata gacgtccgcc gccggtatct
    10681 ccccgagccg ggcgctgcgg gcgaggtcgg cgaggatgcg cgcgtcgccg gtcatgtcgc
    10741 gcaccgcggt gacgccgcta tggacttcgc gccgcagcgc cgcctcggcc gcgcgccggc
    10801 cctggccgat ggcgagatgc tggtggacgt cgatcaggcc cggaatggcg aacagcccct
    10861 cggccgcgac ccgctccgcg ccggcgggca gcgccgccgc cgccggctgc accgccgcga
    10921 tccgctcgcc gcgcagccca ggatcgaggg gctgctcgag accgcgctct acgtggatga
    10981 catggcgcgc tcgcgccgct tctttgccga agtgatcggc ctcgcgccga tgttcgaaag
    11041 cgagcggctg atcgccttcg acgccggccg gcagggcgtg ttgctggtct tcaagcgcgg
    11101 agcgtcggtg gaggatatgc cgaacccggg cggcctcgtc gccggcagtg acggcagcgg
    11161 cccgctgcac atggccttcg ccatcgcggc cgagagctac gacgaatggc attctcacct
    11221 aatcgcttcg ggagtgacga tgcggggcga gatgcgctgg ccgcgcggcg gccgcagcct
    11281 ctatttcgag gatccggacg ggcacgtgct cgagctcgct acccccggcc tctggccgaa
    11341 ttactgatca ggcgagcgcc tcggcgcgct ggccgatcag ccgcaggtcc tcgacgaagc
    11401 gggcatattc cgcttcgcga ttgtcgcgga cgcggaggag gaagctcggg tggaccgtca
    11461 cccagccttc gccgccgtcg tagatggggt gcgggcggcc gcgcatcgag ccgatcgtga
    11521 ccgccttgcc gaacagcgac cgcgcggcgg tggcgccgag cgcgactgtg atcggcggcc
    11581 ggatcagcag ccgctcctgc tcgatccacc agcggcaggc gtcgatctcg gcgccgttgg
    11641 gcttggagtg gatgcggcgc ttgccgcgcg cctcgaactt gaaatgcttg accgcattga
    11701 tgacgtaggc ggcgccgcgg tcgaccccgg cgtcgccgag cgcgcggtcg agcagctggc
    11761 ccgcaggccc gacgaacggt ttgcccgcca gatccacctg gtcgccgggc tgctcgccga
    11821 cgaacatgat gcgcgcgtcg agcggcccct cgccgaacac cgtctgagtg ccggtcttgt
    11881 agagcgggca gcgggtgcag ccgagcgcct cgtcccgaat cgcttcccac gccgcgcgga
    11941 cgttgccgcc gatgccgctc ctcgaactct ccaccatctg cgcctcgcgc gcctgcgctc
    12001 ctgcgatcaa tcccccgacc aacgcggtct cgggcatgtt tttccaatat ttcttcggca
    12061 tctccttggt catggcgccg accttcagcc gcgccgggtt gaagatcgag gcataatagg
    12121 tcttccacgt ctcctcgacg gggtcgccgt ccggcgcatc cgcgcgcgtg gcgccgggcg
    12181 cttcggtcag ggtcgcgccg tcccagtgga gcgacagcgc cggcgtcagg atcgaccagg
    12241 gcatgttggc gaagcgccgc acgaagaaac cggcggcggc gcggacgatg tgatgatccg
    12301 gctcgaacca ggcgacgtag cgggcgccgg cctcggtcgt cacttcgcgg aagcggagga
    12361 aggcgtgcat cttgtggata tcccgccgca cctccttcgc catccgctcg agacgcccgg
    12421 ccagcggatc ggcgctgtcc tccatcgctc ccggctcctg cttgaggcgc aggagcaggg
    12481 tgtagagcaa ggcgaagcgc tccgggtccg aatggcagat cgcgctgcgg gcaagctcga
    12541 tgaacgggcg cggcacgctg aagcccgccg aagcactgcc ttcgaatggc ggttcgccac
    12601 cgctcatggc gaacaggtcg cccacgccct cgccggttcg ccaggcgatc cggtgcggcg
    12661 gctcgccggc gagcgcgagg ccgcgggcgg cgtcgcgcca gccctcgaaa tcgtcctcgg
    12721 ccgcgagcgt caccacccgc atggcagatg attccaatgg ctttgagcag ccgaggcttc
    12781 aatgctatgg gccgccgctc ccgacgcccg cgaggtcgag agatgacgga aggtccgcag
    12841 ccgacgctcg gcgcccggga actggccgcg gtgacggtcg gcatcgtgat cggcgcgggc
    12901 atcttccgga cgccctcgct ggtcgcgggc gcgtcgggca gcgaagccgt cttcctcatg
    12961 acctgggcgg ccggcgggct gctctcgatc gtcggcgcgc tctgctatgc cgaactcgcc
    13021 tccgcctttc ccagcgccgg cggcgactat cacttcctgg cgcgcgcctt cggccggcgg
    13081 cttggcttcc tctacgcctg ggcgaggctg gcggtgatcc agaccgggtc gctggctttg
    13141 ctcgccttcg tgttcggcga ttatatgagc gcgctggcgc cgctcggctc cgcctcgccg
    13201 gcgctctacg cggccgcggc cgtgatcggg ctgactgcgc tcaactgggc ccatgtccgc
    13261 ggcggcaccg gcagccagct ctggctgacc gcgatcgagg tcggcggcct gatcgcgctg
    13321 atcgggatcg ggctcggcat ggccggcatg gcggcgcctt cggaggcccc gtcgccggaa
    13381 gccggatcga tcggccttac cctggtgttc gtcctgctga ccttcggcgg ctggagcgag
    13441 gcggcctatc tttcggcgga gctgcgcggc ggccgacggc gcatagcaac cgtgctggtc
    13501 ggcagcctcg ccttcgtcac cttgctctat ctgctggtca actgggccta tctccgcgcg
    13561 ctcgggctcg gcgggatcgc ggcctcggac gccgtggccg ccgacatggt gaggcccgtg
    13621 gcgggggcgg ccggcgtggc ggcgatcagc ctgctggtcg cgatcgcggc gctgacttcg
    13681 gccaacgcca ccgcgatcac cggcgcgcgc accacctatg cgctcgggcg aagctttccg
    13741 cggctgcgat ggctcggccg ctggcacgcc gccagcggga cgccgcgcaa cgccttgctg
    13801 tttcaaggcg cggtcgccct gctgctgatc ggcgccggcg ccttcgcccg cgacggcttc
    13861 cagctggtgg tcgaatatac cgcccccgtc ttctggctgt tcctggtgct ggtgggcatc
    13921 agcctgttcg tcctccgcgc gcgtgaaccc aacgccgaac ggccgttccg ggtgccgctc
    13981 tacccgtggc tgccgatcgt cttctgcatg accagcgcct ttctcttcta ttcgagcctc
    14041 gcttataccg gtgccagcgc cctcgtcgga gtcgccgttc tggcggccgg cgggctgctg
    14101 ctcctgttcc tggaacccga tgcggatgag gagatgagcc catgaaaatc ctgcttgcca
    14161 tgcttgccct cggcaccgcc gccgcggcgg gggccgttca cgtctcaggc gcgggccagg
    14221 ccgagcccag cggaaccgcg gcgcccgcgg cccagcgcga gcccgacgtc atctacgtac
    14281 cgaccccgcc gccggtggtg caggcgatgc tgaggctggg cggggtaaaa aaaggcgacc
    14341 tgctctacga tctcggctcc ggcgacgggc gcatcccgat cgccgccgcc aaagtctatg
    14401 gcgtgcgcgg cgtgggcatc gacatcgatc ccaagcggat cgccgaggcc aacgccaatg
    14461 ccaaagcggc gggcgtcgcc catctcgtca gcttcagaca ggccgatttg ttcgaaaccg
    14521 actttcacga agcgagcgtg gtgacgctct atctgctgcc gagcctcaac gagaagctgc
    14581 ggccgcggct gctcgcgcaa ttgaagccgg ggacgcggat cgtcagccac gccttcgaca
    14641 tgggcgaatg gaagccggag gtgacccaac aggtcgaagg ctccaccatc tatttgtgga
    14701 ccgtgccgcc gcggcgctga gcggcggccg ctcatgcggc gaacatttcc agctgctcga
    14761 cattcggccg cagccgccgc gcgaggtcgg cgcggtcggt gaggagggtg gggcgccagc
    14821 cttccgcgac gaggaacggg cggaccttgg tcaccgaaac ggtgagacgc gcgacatcgt
    14881 cgagggtcag ccggcgccag cggcgcgagg tgaggatctt gtcgaccgcc ttcacgccga
    14941 gcccgggtac gcgcagcagc cgttcgcgcg gcgcgcggtt gacgtcgacg gggaaatgct
    15001 cgcgatgctt gagcgcccag gcgagcttcg gatcgatgtc gagcggcagc attcccgccg
    15061 ggtcggcggc ggccgcgacc tcggccggcg catagccgta gaatcgcatc agccagtccg
    15121 actgataaag gcggtgctcg cgcatcagcg gcgggcgctg gagcggcagc acggcgctgg
    15181 cgtcgggaat cggcgagaag gccgaataat agacgcggcg gagcgcgaag tggtcgtaaa
    15241 gctggctggc gcggcgaata atctcgccgt cgctggcgct gtcggcgccg acgatcatct
    15301 gcgtggattg gccggcgggc gcgaaagcgg gggccgattt gaagcgcctc gcggcgtcgg
    15361 cgccgtcgtc gatcgccgtt ttcatgtcct tcatcgccgc ttcgatgcgg gcgccgtcct
    15421 tgtccggagc gagccgctcg agcccgcggg cggtgggaag ctcgacgttg atcgagacgc
    15481 ggtcggcgtg gaggccggcc tgccggatca gctccggatc ggcgtcgggg atggtcttga
    15541 ggtggatgta gccgtgaaag tcgtggtcct cgcgtaggct tcgcgccacc tcgacgatct
    15601 gctccatcgt gtagttggag gagcggatga tgcccgagga gaggaacagc ccctcgatat
    15661 aattgcgctt gtaaaaagcg agggtgagcc ggacgacctc ctccgcggtg aagcgggcgc
    15721 ggcggacgtt cgagctcttg cggttgatgc aataatggca gtcgaagatg cagctgttgg
    15781 tgagcaggat cttcaggagc gagatgcagc ggccgtcggg ggcgtaagcg tggcagatac
    15841 ccatgccctc ggtcgagccg atccccttgc cgcccgcgga gcttcgtttg gcggtgccgg
    15901 acgaggcgca ggacgcgtca tatttcgcgg cgtcggcgag gatggcgagc ttcgcctgaa
    15961 cgtcgagctg gggcatgtgt tcgatatatg ttccgtaaca gcctctgtcg agagggaagg
    16021 gggtaaactt gtcatacctg tcactcagaa gagcatagaa gcggccgggc ggtcggggac
    16081 ggagccacgc gcgcgtcgcc ccggcgccgt tgcggccgac acaccccggg tgcgaaagtc
    16141 gttgaaaatt ttgcattatt gcgattcaag ctattgaact ataaggcctt ccctgccatg
    16201 gggcggccag cggccaaggg gccgcttttt tttgtggggg ttatccttgt gaataatatt
    16261 atgcgccttt gcggcgtggc tatggtcacg ctgctccttc ccgcctgtgc caccgtcacg
    16321 cgcggaacca agcagaccta tgtcatcgaa tcgagcccgc ccaacgcgca ggtcgcgctg
    16381 tcgacgggtc agaagtgcgt cacgccgtgc cgcctgaagc tgaagcggaa gtacgacttc
    16441 accgcgacct tcaccaagcc gggctatgac cccgccaccg cgcaagtgcg gagcaagctc
    16501 tcgaccggcg gcggcgtggc cgcggccggc aacatcatcg ccggcggcat catcggcggc
    16561 atcgtcgacg gcaccaacgg ttcgaccaac gacctgacgc cgaacccgct ggtggtgacg
    16621 ctgaagccga acggccagcc caccgcggca gcgcctgcgg ccgggtcgcc gacgggcggc
    16681 aaccgctaaa cgtcggttcg acacaaaaaa agggggcggc gccaggagcg ccgccccctt
    16741 tttcgtgcgt gggcgaggga gctagctggc cggtccgatg ctcatccggc tgtcgccgcg
    16801 ccatgagaag ccggtggccg aaagcatcga aggcgcgtcg agcggagccg ggagcgcctt
    16861 ttcgaccgga gcggccggag cagtcacagc cgccgcgagc cgggtttcgg tccggccgcg
    16921 agacggctcg gcggcggcaa gcgccacggc aggctcggga gcggggtagc gggtgaaggc
    16981 gttgagctcg cccggcgcgc cccgccagcg atagaagatg tgcgcgccaa gctcggtgat
    17041 gcgggtcagc gtcccggccc aataaggatt gacctgattg gtatgataat gagtggcggt
    17101 gccgaccggc gcgtagacgc ggccggcgag ggcctcctcg gcgatctgga gcgcctcttc
    17161 ccaagcggcg ggcacgcggc ggcggcggag cgatccgtcg caggtgaagg tgaactggca
    17221 gccggtgcgg cggccggcgc cctggaagac gactccgcag acggtggccg ggaagctggg
    17281 atggcggacg cggttcagca ccacctgcgc caccgcccgc tggccggcct cgccctccga
    17341 gcgcgcctcg tgatagaccg ccgcggcgag gcaatcgagc gagcggcgct gggtggcgct
    17401 gcccgcctgg acgatgcgga acgggcgcgc gggctggagg gctccgacgt ggatcaagtc
    17461 gccgtctacg gacagcacct cgtcattcaa caggccttcg agattggcga ccagcaagga
    17521 tgcgggcgcc agctcgatcg agaggccgtt cggagacagg cccggcttgc tcagcgcggt
    17581 cgtgctcgcg gtcagcgcca gcaaagagga agcggcggcg accaaagccg gcagagccaa
    17641 cgtcctttcc ttgccgaaat tccccacgca gccaccccta tgtcgagagc cttgagaggc
    17701 ggggtgtacg cccgattggt tagcgtcggg ttaaggctgt tgagggggaa gcgagccggc
    17761 gagcgcgtgc gccgggagtc agaagctcat ctcttcgtag acgcgggaga cgtcgccctc
    17821 ccattcgccg tggaagctgg cgaggaggcg atcggcgggg gtcgcgccgc gggcgacgat
    17881 ctcgcgtagc ggatcgagga agccgctctc attgtcgccg gacgcgttga ggcgcgcgcg
    17941 ggcgttgagg ccggcctcgg cgatggcgag gatgcgctgg ccgagctgct ggagcgtctc
    18001 gccggcgggc gtgcgggcgg ctaggccctc tttcggcacc gcgtcgcgaa gctgctggcg
    18061 ctcttcgagc gtccagtgcc ggacctcgtc ccaggccgca tcgagcgcgg cgccgtcgta
    18121 gagcaggccg acccagagcg cgggcaaagc gcagatccgg ttccacgggc cgccgtcggc
    18181 gccgcgcatc tcgagaaagc tcttcagccg gacttcggga aaggcggtgg acagatggtc
    18241 gttccagtcg gacaggagcg gcttctcgcc cgggagggcg ggcagctcgc ccttcaggaa
    18301 atcgcggaac gactggccgg cggcgtcgac atagcggccg ccgcgataga cgaagtacat
    18361 cggcacatcg agggcgtagt cgcggtagcg ctcataaccg aagccgcttt cgaacacgaa
    18421 gggcagcatg ccggtgcggg cgccgtccgt gtcggaccag atgtggctgc gataggagag
    18481 gaagccgttg ggccgccgct cggtgaaggg cgaattggcg aacaaagcgg tggcgagcgg
    18541 ctggagggcg aggccgactc tgaacttctg cgccatgtcc gcttcggacg cataatcgag
    18601 gttcacctgg atggtgcagg tgcgcagcat catgtcgagg ccgagcgtgc cgaccttggg
    18661 catgtggttg agcatgatcg cgtagcggcc cttgggcatg cgcggcagcg cgtcgcgggt
    18721 cttgtccggc cacatgccga gcccgaggaa gccgaggccg agcttctcgc ccaccgcctt
    18781 cacctgctgc aggtgccggc cggcctcggc gcaggtctgg tggagatgct cgagcggcgc
    18841 gccggacagt tcgagctggc cggccggctc gaggctgaca ttgccgtcgg cgccggacag
    18901 ggcgatcacg ttgccgttct cgacgaccgg ctgccagcca tattgggtga gctcgctcag
    18961 gaggtcgcgt atgccgccgg gctcctcata ggagggcgcg cggtggtcgc tgcggcggta
    19021 gacgaacttc tcatgctcgg tgccgatccg ccagcgctcg ggcggcttct cgcccctggc
    19081 gaacggcgcc agcaggtcct cgatcgattc gacgcgcggg tcggcatcgt cggaggcggt
    19141 tcgcgtcgtc atcgcggccc catagcgagg cgggagactc ctcgccagtc ggtttcggtc
    19201 cgctaccgag tggagtcccc ggtccagtcg cccgacatcg ccatccacaa gctgatcgcg
    19261 gcgagggcgg cggtgtcggc gcggaggatg cgcgggccga ggctggtcgg gagggcttgg
    19321 gggagggcgc ggatggcggc gcgctcctcg tcggtgaacc cgccctccgg gccgatcagg
    19381 atggcggcgg gcccggcgcc ggggccaagc ggctcgccgc cctgctcgtc ggcgaagatc
    19441 agggcgcggt cctcgggcca ggcggccagc aaggccggca gcttttgcgg ctcggccagc
    19501 tcgggcaagg cggtgcgttc gcattgctcg gcggcttcga tcatgtgggc gcgcaggcga
    19561 tcgaggttga ggcggtcgac gatggtgcgg cgggtcagca ccggcaccag ccgcgcggcg
    19621 ccgagctcgg tcgccttttc gaccagccag tcgatccggc cgcgcttgac cggcgctaac
    19681 agcagccaca ggtccggcac cgcctcccgc tcgcgaagcc ggctgaggat tgtgagcgcg
    19741 acgcgccgct tgccggcctc ggaaatctcg gcgagccact cgccggtgcg gtcgtcgaac
    19801 aatttcacct gcgcgccggc ggcgaggcgg aggacgttgc cgagataatt ggcgggggcg
    19861 ccgtccaggg aaagcgccgc gccttcggaa agcggctggt ccacatagag gcgcggtagg
    19921 ctgtgcggcg gccaggcggg ggtggcgggc atgccggttg cgtagccgtt ctggccgggc
    19981 ctgtcgaagc ccagccttga tttattccca agggagaagg acagcccttc gacaagctca
    20041 gggcgaacga gagcccttcg acaagctcgg gacgaacgac agtggggatt gaggccggag
    20101 agaccgacat cgtgccggac agcgagcggc gggggctgat cggggcgctg ccgcgggcgg
    20161 tgcggcccta cgcctcgctg atgcggctcg accggccaat cggggcctgg ctgctgtttt
    20221 ggccgggcgc gtggagcgtg gcgctggccg gcatgggcag ccggggggtg gcgctgatcc
    20281 tgtggctggg gctcggcgcc ttcgcgatgc ggagcgcggg ctgcgtctat aacgatatca
    20341 tcgaccgcga gctcgaccgc caggtggcac gcacgcggct gcggccgctg gcgagcggcc
    20401 gggtgccgct caaaggggcc tgggcgctgc tggtcgggct gagccttatc ggcctgatcg
    20461 tgctgctgca gatcgggccg ctcgcgcagc tcgtcgcgct ggcgagcctc gcgctggtgg
    20521 ccgcttatcc gtttatgaag cggatcacct ggtggccgca ggcgtggcta ggcctggtct
    20581 tctcctgggc cgccctggtc gggtggccgg cggtggaggg cgagatcgat gcggccgcct
    20641 tgtggctcta cgccgggtcg gtcgcctggg tgatcggcta tgacacgatc tacgcgctgc
    20701 aggatcgcga ggacgacgct ttggtcgggg tgaagtcctc cgcgctggcg ctcggcgagc
    20761 gggcgcgggc cggcgtggcg atcttttacc tcattgcgct ggcggcctgg ggcgcggcga
    20821 tctggcaggt gcgggcggac tggctgggcc tggcggcgct gctgccgatg gcgctgcacc
    20881 tgatgtggca ggtggcgacg ctgcggcagg aggacggcgc cgacgcgctc gccaagttcc
    20941 ggtcgaaccg ctttgccggc ttcatcatgt tcctcgcctc cctggtcgtg ggtacgacct
    21001 agcttttacg ggcggcggcc cttaagcggc ggccatgctt accgccaaag aagccgaagc
    21061 gcgcgccggc gcgctgatcg aagccgccat gaaggccggg gccgacgccg ccgacgtgct
    21121 ctatgtgggc gacggatcga ccggggtgca ggtgcggctc ggcgagctcg aggatgtcga
    21181 gcgttccgaa ggcgaggaga tcgggctgcg cttcttcgtc ggcaagcgct cggctggggt
    21241 cgcttcgtcc gatttgtcgc gcgaggcgct ggcggccttg gtcgagcgcg ccgccgcgat
    21301 ggcgcgcgag gcgcccgagg acgaatatgc cgggctcgcg cccgagcacc ggttgctgcg
    21361 cgacggcggg ccggacatag cgggcgacga cgggcgcgat cccgcgccgg ccgaattgaa
    21421 ggcgatgtcg ctggcgatcg aggcggcggc gctggcggtg ccgggtgtga ccaacagcca
    21481 gggcgcgggc gtgagcgcgg gccgttcggt ggtggcgctg gcgaccagtc atggcttctg
    21541 ccgcggctat acgaccagcg gctattcggg atcggccagc gtgatcgccg gcagcggcgc
    21601 caacatgcag cgcgattatg ccagccacgg cgtccgtcat ctcgaggatc tcgacgatcc
    21661 cgagacgatc gggcggctgg cgggcgagcg ggcggtggcg cggatggacc cgggcaagct
    21721 tcccagcggg ccgatgccgg tggtgttcga tccgcgcgtc ggcggcagcc tggtcaacca
    21781 tctgctgggc gcgatcaccg gctcggcgat cgcccgcaag accagcttcc tgctcggccg
    21841 cgagggcgag ctcgtcttcc cggaaggggt gacgatccgc gacgatccgc accggcggcg
    21901 cgggctcagg tccaaggcat tcgacggcga gggcatggcg acgcgggcga ccgacctcgt
    21961 cgatcgcggc cgcctcaccg gctggctgct cgacagcgcc tcggcgcggc agctcgggct
    22021 cgagccgacc ggccactcga cccgcggcgt cggcggttcg cccggcgcca gcgcgaccaa
    22081 tgtcgagatg ctgccgggaa cgctgagcaa ggcggagctg atcgccgata tcggccgcgg
    22141 cctctacgtg accgagctga tcgggcaggg cgtcaatccg gtgaccggcg actatagccg
    22201 cggcgcctcc ggcttcatca tcgagcaggg cgagatcacc gtgccggtgg cggagatcac
    22261 catcgccggc aatttggtcg acatgttccg cgagctggtg ccggccagcg acctcgagca
    22321 tcgccgcgcg gtcgacgtgc cgacgatccg catcgacggg atgaccatcg ccggtgcttg
    22381 acgcggtcgc gaagatcgcg gccgaagcgg gccgcatcgc ccatgcgaaa agcggcagcg
    22441 ccttccgcca gtgggagaag gtgccgggcc acccggtctg cgacatcgac atcgagatcg
    22501 acgccttttt gcgcgagcaa ctggcggcgc tcgaccccga ggcggggtgg ctttcggagg
    22561 agacgctcga cgcgtccgac cgctacgagc ggcggcgcgc ctgggtggtc gatccgatcg
    22621 acggtacgcg ggactatctg cgcggccgca gcggctggtg cgtgtcggtc gctttggtcg
    22681 aggaccgggt gccgctgatc ggcgtgctcg acgcgcccgc ccgcggcgag gtctggaccg
    22741 ccgagcgcgg ccggggcgcg tggcgcaacg gtgcgcggct gcgggtcagc gagcggcgcg
    22801 agctggccgg ggcgcgggtg ccgaccgagc ggctggctac gcccgatgcc gacctgacgc
    22861 cggtcgcgaa gccgaactcg atcgcgcttc ggatcgcgat ggtggcggcg ggcgaggccg
    22921 atctggtcgc aaccctgcgc tggggcttcg aatgggacat cgccgcggcg gcgctgatcg
    22981 cgagcgaggc gggcgcgacg gtgaccggcg cgctcggcca gccgttcgca ttcaacaccc
    23041 attccggcga agccttcgga gttctggcgg cgacgcccgg catccacgcg gcggcggcgg
    23101 cgcggctgcg cgagcgggcc gaggcggcgg tggggtaggt gggttccccg gcgaaggccg
    23161 gggtcaggtc cggccatcct ctggaccccg gctttcgccg gggaacaaat tttacccgat
    23221 gcgcggcacg cggagccggg ggatctcgat cgcggggcag cggtccatca ccaccttgag
    23281 gccggccgct tcggcgcggg cggcggcttc ctcgttgatc acgccgatct gcagccagac
    23341 cgatttggcg ccggccgcga tcgcctcgtc gaccgcttcg ccggccgcga tcgggcggcg
    23401 gaaaatgtcg accatgtcga tcggctcacc gatttgcgac agttcgcgcc agacatattc
    23461 gccatggaca tgctcgccgg tgatctgcgg gttgaccggc agcacgcggt agccgtgatc
    23521 ctggaggaag cgcatcacgc cgtagctcgg gcggcccggc cggtcggacg cgccaatgag
    23581 cgcgatggtg cgggtctcgg cgagcagctc ggcaatgtcg cgatcggcgg tgaggggcat
    23641 ggttcctcct gcttcgggga tagtcggaca atggttcagg ccggggttcg gttcatctct
    23701 tcgatgaggc gcgcggcgat ggcgcggtaa tagccggcct cctcggcgtc gccggcggcg
    23761 ggcgggacgc cttcgtccga cgcgcggcgg atggcgaggg tgagggggac gcggccgagg
    23821 aacgggatcg ccatcacccg cgcggcggcc tcggccccgc cggagccgaa cgggtcggag
    23881 acttcgccgc aatgcgggca ggcatagccg gccatatttt cgacgaggcc gaggatcggc
    23941 acgtgcatct tgcggaacag gtcgatcgcg cgggtggcgt cgatcagagc aaggtcctgc
    24001 ggcgtcgaga cgatcacggc gccggcgggg cggtatttct ggatcagcga gagctggatg
    24061 tcgccggtgc cgggcggcag gtcgacgatc atcagctcgg tgtcgcccca gtcgccgtcg
    24121 agcagctggc cgagcgcgcc ggccaccatc ggcccgcgcc aggcgagcgc gatgccggcc
    24181 tcgaccaact ggccgatcga cagcatgcgc acgccatgag cgagcgtcgg cacgatctgc
    24241 ttgcccactg cctcgggctt gtcgtggctg cccatcaggc gcggctgcga gggcccgtaa
    24301 atgtcggcgt cgacgaggcc gaccttgcgg ccggtgcggg cgagcgccac ggcgaggttg
    24361 gcggcgagcg tcgatttgcc gaccccgccc ttgccgctgc cgaccgcgat cagcttgcgc
    24421 tcgaccttgt cggcggtcat cgcgatgcgg atctcgtcga tgccgggctc ggcggcgagg
    24481 ctggcgcgga ttgcccgttc gagctcggcc cgtgcctcgg cgccgaggcc ggtcgcatcg
    24541 acgatcatgc tggcgacgcc gccctggagg cgcggcgccg agacgcggcc cgagtcggcg
    24601 agatcgccgc cggccgggtc cgggacccgc gccagcgcgc gcatgacggc gccttcattg
    24661 tcgctcattg cgggagcgat aggcatatcc ttggccgatg ccactgtttt tccgacgggc
    24721 gcctacctat aaacagttcc atgagaagaa tcttcgggtg gggcgcgcgt cccggcgccg
    24781 ttttcaatga gaatagaggc ggcccctggg gcaccggcgg cggcggcgga ggcgacggcg
    24841 atgggccgcg caatccctgg ggccagcctc cgcgcaagcg tcgaccgggc ggcggcgccg
    24901 gcaacgtcac ctccctcgac gaattcctga agaagagccg gcagcgcatc ggcagcggcc
    24961 tgccgcaggg cgacggccgg ccttactggc tctacggcct cgccatcttc gtcctgctgt
    25021 ggctgctgtt caccagcatc caccggatcg gcccgcagga gcggggcgtg gtgacgcggc
    25081 tcggcagcta tagcgggacc atggagccgg gcatcgcctt ctccctgccg gcgccgatcg
    25141 acacggttcg caaggtggac gtcgaggaga tccgcacgat cgacatcggc tcgaccgatg
    25201 cttcgagcga gaatctgatc ctcaccgccg accagaacat catcgacctg gcttattcgg
    25261 tgcgttggaa catcagcgac cccgaactct atttgtttca gatcgccaac cccgaagaaa
    25321 ccatccgcga agtcgccgag agcgcgatgc gggcggtggt cgccggcgtg tcgctcgacg
    25381 acgcgatcgg cgccggccgg ggcgagatcg agcagcgcgt gcagacggtg atgcagcagt
    25441 tgctcaacga ttatcgtgcg ggcgtccgcg tccagggcgt cgcggtcaag cagtcggacc
    25501 cgccggcggc ggtcaacgat gcgttcaagg aagtgtcggc tgcgcagcag caggcgcaat
    25561 cctatatgaa cgacgcgcgc gcttacgcgc tccagcttac cgccaaggcg cagggcgagg
    25621 cggccgcgtt cgacaaggtg ttcacccagt acaagctgtc ccccgaagtg acccggcggc
    25681 gcatgtatta tgagacgatg gaaaaggtgc tttcgcgcgt ggacaagacg atcatcgaag
    25741 cgcccggcgt gacgccgtat ctggcgcttc ccgaagtgca gcgccgctcg caatcccagg
    25801 tgcaggcgca gccgcagccg cagccgcagc cgcagccgca gcagggaggg accggccagt
    25861 gatccagcgt ctcggccgca atccgatcgc gctcggcgtc ctcgcggtcc tcctcctgat
    25921 cctggtcttc agcaccttct cggtggtgcc ggagacgcgg caggcggtga tcgtgcgctt
    25981 cggcgagccg gaccgcatca tcaacagcta ccggcccaac acgccgttcg ggcagaccgg
    26041 ggccgggctc atcgcccgca tcccgttcat ggaggagatc cactggatcg acaaacgcat
    26101 cctgtcggtc gagatggacc agcagcaggt gctttcgacc gaccagctgc ggctccaggt
    26161 cgatgccttc gcgcgctatc gcatcgtcga tccgctgcgg atgttcatct cggcgcggac
    26221 cgaggagcga gtcagcgacc agctgcggcc tatcctcggc tcggcgctcc gcaacgagct
    26281 cggcaagcgg ccgttcgccg ccctgctgag ccccgagcgt gggcagatga tggacaatat
    26341 ccagcgcgcg ctgaaccgcg tcgcccgcca atatggcgcc gagatcgtcg acgtgcggat
    26401 caagcgcgcc gacctgcccg aaggcacccc gctcacctcc gcctatcagc ggatggagac
    26461 ggcgcgccag caggaggccc gctcgatccg ggcgcagggg ctgaagcagg cgcagatcat
    26521 ccgcgccgag gccgacgccg aggccgcccg cacttatgcc gaggcgttca acaaggatcc
    26581 ggatttctac gatttctacc gcgccatgca gtcctacgaa gcgagcttcg tcgataacgg
    26641 cgccgggcag gagacgatgg gacggtcgag catcgtgctg tcgcccagca acgactatct
    26701 cagggagttc cgggggcgcc ggtaaccgac acgcggatcg gccagccatt caatccccgt
    26761 tcagccgcct cacctagctt gcggcacgct gaacggcccg tccgggtcga accggcgggg
    26821 ccagacattt gaggagatga gcacagtgcg ttacgtctat ggcatcaccg ccgccctgct
    26881 tgccggcggc gccgtcg
//
LOCUS       DASVRK010000305         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_308608, whole genome shotgun sequence.
ACCESSION   DASVRK010000305 DASVRK010000000
VERSION     DASVRK010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308608"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VF589_12810"
     CDS             <1..1316
                     /locus_tag="VF589_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX8258504.1"
                     /translation="HLLSVSGLHLTAVVGAVMLLTLKLLALSPALALRFRLTVAAAAA
                     GALAGIAYTLLTGAEVPTIRACVAAILVLIGVAIGRDALTLRLVAVGALIVLLFWPES
                     LAGPSFQLSFAAITAIVALHEHPRIKALLARRDEGGFHKIGRFLLALVLTGLSVEIAL
                     TPIALYHFHRAGFYGAAANVVAIPLTTFIVMPLEALALLFDLAGLGAPFWWLAGQALS
                     LLLGLAHAAANAPGAVAMLPTMPVAAFALMVAGGLWIALWRTGWRRWGVVPVAAGAAW
                     ALATPAPDLLVTGDGRHLALRTADGGMAILRGRAGDYVRDMLSETSGYEGDLPAIADL
                     PGAACSPDLCVADIVRGGRRWRLLATRSSRLVDFAPLARACAAADIVVSDRRLPRTCV
                     PRWLKADRALLRRTGGLAITLGETPRVATVADRVGRHPWAAAAER"
BASE COUNT          180 a          630 c          557 g          263 t
ORIGIN      
        1 cgcacctcct gtcggtcagc ggcctccacc tcaccgcggt cgtcggcgcg gtgatgctgc
       61 tgacgctgaa gctgctcgcg ctcagccccg cgctggcgct tcgcttccgc ctcaccgtcg
      121 ccgccgccgc cgccggggcg ctcgccggca tcgcctacac cttgctgacc ggcgccgaag
      181 tgcccaccat ccgcgcctgc gtcgccgcca tcctggtgct gatcggagtt gcgatcggcc
      241 gcgacgcgct caccctgcgg ctggtggcgg tcggcgcgct gatcgtgctc ctgttctggc
      301 ccgaatcgct ggccgggccg agcttccagc tgagcttcgc cgcgatcacc gcgatcgtcg
      361 ccttgcacga gcatccgcgc atcaaggcgc tgctcgcccg ccgcgacgaa ggcggcttcc
      421 acaaaatcgg ccgcttcctg ctcgcgctcg tcctcaccgg gctgtccgtc gagatcgcgc
      481 tgaccccgat cgcgctctac catttccacc gcgccggctt ttacggcgcc gccgccaacg
      541 tcgtcgcgat cccgctcacc accttcatcg tcatgccgct cgaggctttg gccttgctgt
      601 tcgaccttgc cggcctcggc gcgcctttct ggtggctggc cggccaagcg ctgtcgctgc
      661 tgctcggcct cgcccatgcc gccgccaatg ccccgggtgc ggtggcgatg ctgccgacca
      721 tgccggtcgc cgctttcgcc ctgatggtgg cgggcggcct gtggatcgcc ttgtggcgca
      781 ccggctggcg ccgctggggc gtcgtgccgg tcgcggccgg agcggcctgg gcgctggcca
      841 cgcccgcgcc cgacctgctc gtcaccggcg acggccgcca cctcgcgctc cgcaccgccg
      901 acggcggcat ggcgatcctg cgcggccgcg ccggcgacta tgtccgcgac atgctcagcg
      961 aaacctccgg ctatgagggc gatctgcccg ccatcgcgga cctgccgggc gcggcctgca
     1021 gcccggacct gtgcgtcgcc gacatcgtcc gcggcggccg ccgctggcgc ctgctcgcca
     1081 cccgcagcag ccgcctcgtc gatttcgccc cgctcgcccg cgcctgcgcc gccgccgata
     1141 tcgtggtctc ggaccggcgc ctgccgcgca cctgcgtgcc gcgctggctg aaagccgacc
     1201 gcgccctcct gcgccgcacc ggcggcctgg cgatcaccct gggagagacc ccgcgcgtcg
     1261 caaccgtcgc agaccgtgtt ggccgtcacc cgtgggcggc ggcggccgag cgctgaaatc
     1321 ggcgaggtgc cgcagcccga tctcccgaga ggaatttcgg cgccactccg tcattccagc
     1381 gcaagctgga atgttgcggg ttgggtatcc ctgcgggccg aaagcagtgg gattccagct
     1441 ttcgctggaa tgacgacggc gtgtcgcagc tcgcgggcgg gcggagttcg acgcggcgac
     1501 cgctgggaac gggccttcat gctggaaagc aacggctgcg gccgcgatct tggaggcccc
     1561 acaaagtaaa agccccggcc tggggaggcc ggggctctgt ggcagtcagc gggggggggg
     1621 gggggcgctg
//
LOCUS       DASVRK010000306        25095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_309132, whole genome shotgun sequence.
ACCESSION   DASVRK010000306 DASVRK010000000
VERSION     DASVRK010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 25095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25095
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309132"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2803)
                     /locus_tag="VF589_12815"
     CDS             complement(38..2803)
                     /locus_tag="VF589_12815"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX8258505.1"
                     /translation="MAHRPHPIPSRRSIIDRRVLAERLEELRALPEQGRKLAAKSMLK
                     QALADGRGEIARRLEEKPYAGSEIAAAYAFLTDQLLRLVHDFVTGQLYPLGTPTTSER
                     LLLMAVGGYGRGEMALHSDVDLAFVTPWKPTGWTEQVIEAMLYLLWDLGLKLGHSSRS
                     VDDLVRLAGSDHTIRTAILESRFLWGDEALYDEVQARFWGEVVAGKVDLFVTEKLAER
                     NARHARMGDSRYVVEPNVKEGKGGLRDLHTLFWIGKYAYRVRSVPELVETGLLSAEEL
                     GRFQKAERFLWAVRCHLHCIAGRAEDRLSLDYQREVALRMHYADRPGNAPVERFMRHY
                     FLHAKAVGDLTGLFLAHLDERFAKTGRRFGLPAIRWRPRKLEGFLLDRGRLALPSDDF
                     FADDPVRLIQIFALADKYGLEIHPLAMRAAGRDARLIDAGVRADPRANALFLDVLTSP
                     RDPETVLRWMNEASIFGRFVPDFGRVVAKMQFDMYHHYTVDEHSLRAIGLLSRIERGE
                     LRDEHPLSSAVIHQISSRRVLYAAVLLHDIAKGRGGDHSLLGAEVARELCPRFGLDEA
                     ETDTVAWLVRRHLIMSATAFKRDLSDPKTISDFVAEVTSLERLRLLLILTVVDIRAVG
                     PGVWNSWKRQLLRELFEAAEELLRLGHKQKGRRERVAATQEEVQARLRWDESRFARLA
                     WRFPDAYWLAEPADILEQNARFIDRADRAPAATSPVQTSVHEERGATLLTVYARDQPG
                     LFYRVAGAISLVGGNIIDARIHTSSDGMALDNFLVQDGGGRAFDDPHQLQRLEAAVAA
                     AADGQEPMTERLEAKGLPLARAEAFEVKPAVFIDNRASSRYTVVEVNARDRAALLSAL
                     AQAISQARATIHSAHIATYGERAVDVFYLTDLKGEKIEHPARLKSLHARLLKAAGGAG
                     TRALRAA"
     gene            complement(2871..5477)
                     /gene="mutS"
                     /locus_tag="VF589_12820"
     CDS             complement(2871..5477)
                     /gene="mutS"
                     /locus_tag="VF589_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX8258506.1"
                     /translation="MATQAKPISAPSPMMAQYLAIKAEASDCLLFYRMGDFFELFFDD
                     ARAAAACLDIALTARGEHEGQPIPMCGVPIHAAEAYLARLIRAGHRVAIAEQTESPAE
                     AKKRGHKSVVSRAIVRVVTAGTLTEEALLDARAANWLAAVAKVGDRYGIAAADISTGR
                     FELFSVAPAALDAELARLAAAELVACEPLPGQPVQLRRAGFDSVQAERRLKERFGVAT
                     LDGFGAFDRAALAAGGGLLAYLDEVARESLPFLRPPEPRAQGRHMMIDAATRESLELT
                     VSASGTRAGSLLATVDRTVTGAGARLLAADLGAPLLDRPAIEARLDLVGLFEADAGLR
                     DRLRRQLKALPDVSRALGRIAAGRGSPRDLGQLRDGLDEARRLHDRLAALPDLPAMLL
                     ELLPALTGHGELVDLLARALVPMPPVDAAQGGYIAEGYDAALDDLRATGGEGRRAIAA
                     LEARYRQQTGIAALKIRHNGVLGYHIEVPARNADRLMAAESGFTHRQTLAGVVRFNAP
                     DLHDQAVRVGQAGAHALAAEAAHLEELTAAALARAHPIAAAADALARLDVAAALAERA
                     AEGGWCRPQFAGHVCFDIEGGRHPVVEEAVRTAGGRFVANDCRLSEGERLWLVTGPNM
                     GGKSTFLRQNALIVLLAQAGSYVPASRATLGLVDRLFSRVGASDNLARGRSTFMVEMV
                     ETAAIVAQATERSFVILDEVGRGTSTYDGLAIAWAVVEAIHDRNRCRCLFATHYHELT
                     RLAERLDALSLHHVRAREWKGDLVFLHELAKGPADRSYGLAVAKLAGLPPPVLARAKS
                     VLAKLEEGRAATGGIAAGLDDLPLFAAAAAQEEAAPDPLQAALAEIDADALSPREALE
                     LVYRLKRLAAEE"
     gene            complement(5650..8373)
                     /locus_tag="VF589_12825"
     CDS             complement(5650..8373)
                     /locus_tag="VF589_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258507.1"
                     /translation="MADQQNVVQDEGGHARYFKFDRSEPADAIEAMALAAETGRAGAT
                     AQESARNFLLANAATLGIAPEGAASLELSAGIAPSPEGQALRFESEKRIMDTTTVTYV
                     QTMFGLPIHEAGVSVTTQGPANEVAAASSTLHYDIEAPAPASGLVPLTASGPDASNGA
                     YDDLVRRALGKFGPRARINGTRLMVYRYDAERRLESHPHDKHGLGFCGGIPSLPLEPA
                     PAAIADGTHYIVVDALFTLPDKAWGTMNWRAFIEVDSGAVLYLRALVDCASALVFERD
                     PITKTGLAANLPSASAAALDALRDNVTLSNLSPAVGGTQSLSGTHVRLFDNSAPAIGA
                     PTRVTPFNFDYVSRTNDFAAANAYYHCNRFFQLVGDLGFSIASYFDGTAFPVRVDHRG
                     LGNVVNANCPGNAMANGIGQVNFALAHTGDVANPISIGADWRVVIHEIGGHGILWDHV
                     NSPNFGFAHSAGDSIAAILNDPGSAAPDRFVTFPWVNIGRRHDRPVNGWGWGGANDVG
                     GYSSEQILATTHFRIYRSLGGDAAAPAAKDFAARSTVYLILRAVGQLTPVTNPPSPLA
                     WEGQLETADVGVWNSTSPPGAYAGGAYHKVIRWAFEKQGLFRAPGAPATVEGVAPSVD
                     VFIDDGRNGEYPYQPNHWSCGDIWNRTAPGTGGGLHEEPIVGQTNYAYVRIKNRGSQP
                     ATNVVVKGFHALPGAGLAFPGDWAPMTTAQLAAPNVAAGDMAGVVVGPFQWTPSQVGH
                     ECMFFSVSAQGDAGNIDGRVVGPIPEWRLVPHDNNLGQRNVHPVEAPLTGVQRGIEWE
                     RLPFWIRNHGRKAVRTTVEVKLPAWLAKLGWAFNVPQAARRKLVIKPGEMAKVTLAEA
                     KKGRPIAPEDFARQRDRNIVVTVLQEGIPVGGMTFQLTAPRNVRPKPVRPG"
     gene            8760..11021
                     /locus_tag="VF589_12830"
     CDS             8760..11021
                     /locus_tag="VF589_12830"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX8258508.1"
                     /translation="MSESNVKFSEAEALEFHSRGRPGKIEIIASKPMATQRDLSLAYS
                     PGVAVPVRAIAGDPAKAYDYTSKGNLVAVISNGTAILGLGNLGALASKPVMEGKAVLF
                     KRFADVDSIDIEVDTEDTDAFINAVALLEPTFGGINLEDIGAPACFIIEQALKERMNI
                     PVFHDDQHGTAIITAAGLINACLLTGRDFKDVRVVVNGAGAAAIACTELIKSMGVRHD
                     HVIMCDRKGVIWQGRPDGMDQWKSAHAAVTDARTLHEALVGADVFLGLSAAGALKPEM
                     VMKMAERPIIFAMANPDPEISPPDAREARPDAIIATGRSDYPIQVNNVLGFPFIFRGA
                     LDVRATSINDEMKIAAAEAIAELARQQVPEEVAAAYGGRSHSFGPDYIIPAPFDPRLM
                     ELVPAAVAAAAMRSGVARFPIKDMTAYRTALRSRLNPTTSVLSLAYEAARANPKRVIF
                     AEGEEETVLRAAIAFREGGYGIPVLVGRDDVYDRLRALGVDDPDSFEIQNSRRSERIP
                     EMVDYLYRRLHRRGYLHREVERMVNQDRNTFGALLLALGEGDVMITGVTRPYGQSIRQ
                     IRRVLDPKEGVTPFGIHVLVGQSHTIFMADTTVTERPTAEQLAHIACETAAVARRMGH
                     EPRVAFLSYSTFGNPEGTFLDNVRGAVRLLDGRSVDFEYEGEMAPDVALNPALKPIYP
                     FSRLTGPANVLIMPGLQSANLSAKLLEQLGGDAVIGPMLVGMAHPVQVAAMTASASDL
                     VTLAVLAAGGIVR"
     gene            complement(11044..11520)
                     /locus_tag="VF589_12835"
     CDS             complement(11044..11520)
                     /locus_tag="VF589_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX8258509.1"
                     /translation="MTLRHGRSLAAAMLIGAALLTSACQKVRGRQGYLIDEVLVSAIQ
                     PGVDTRESVQGTLGRPTFVGQFDQRDWYYVSRRTLQMAFNHPRAVEHTVLHIRFDEAG
                     NVVAVDRSGFERVASIDPMNDKTPTLGRDRSFFEELFGSIGQVSTAAPAGSADNPQ"
     gene            11654..12172
                     /locus_tag="VF589_12840"
     CDS             11654..12172
                     /locus_tag="VF589_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX8258510.1"
                     /translation="MSFLARIFGEKEDREALGPLYAAVVAAGRDPAWFRQGQVPDTLD
                     GRFDMIAALLTLVLLRLEADGDSGRRASVVLTERFIDDMDGTVRQMGIGDLMVGKHVG
                     RMMSALGGRLTAFRAAGQGDLGEPVARNVFRDAVPSPEALAFVSGRLERYRDALQATP
                     SQALLDGEMPSL"
     gene            12169..12696
                     /locus_tag="VF589_12845"
     CDS             12169..12696
                     /locus_tag="VF589_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX8258511.1"
                     /translation="MSPELSRPVRVDTLGATPRSLSVEADARERAALAERFGMPGIDS
                     LSADVELTLKGEAVIVAGTLKASVTQSCVATGVPVPERVDEPFTVLLRPRPEAGAEEE
                     IELSEGELDVVFYEGAAVDVGEVVAETLSLALEPYPRSPEAAAALKEAGVLSEEEAKP
                     ASPLAGLKDLLTPKS"
     gene            complement(12721..14142)
                     /locus_tag="VF589_12850"
     CDS             complement(12721..14142)
                     /locus_tag="VF589_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX8258512.1"
                     /translation="MPNRLPLLAALLLAGCSQQPKYVQPGLPTAPQYPADAWPVTAGP
                     RAPELPWRGFFRDPRLQALIERALIHNRELRTAVLRIEEARGQYRIRRADRLPTLDAN
                     AGAARSRTGDGFGGAVTGNRFEIGASVASFELDFWGRVRSLTESARANYLSTIHAQRA
                     FQISLVADVAEAYLTERELDDRIAIAERTVRDRERALEIGKLRLDAGVTSALDYRQIE
                     TLLTQAQAALADLQLQRAQNRNALEFLVGGPVGAPLPPPLPLERQGIVETISAGLPSE
                     LLTNRPDILEAEELLRAAYADVGAARAAFFPQISLTGALGFASGALGSLFSGGAFTWS
                     LGGNASLPIFDAGRTRGNLDVAVARQGIAVASYERAIQQAFREVADRLAERRWLAERL
                     TAQQRELAAQRDRAELATLRYQNGVTGYLEVLDAQRELFAAEQQVLATRREQLTNAVD
                     LYVALGGGIEGAAPISAPLRAPS"
     gene            complement(14135..17275)
                     /locus_tag="VF589_12855"
     CDS             complement(14135..17275)
                     /locus_tag="VF589_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8258513.1"
                     /translation="MLARFFIDRPIFAWVIALSISLAGLLAVNALPIEQYPTIAPPSV
                     TIDVAYPGADAATLEQNVTSIIEQEMNGVENFLYMSSSSAANGSATITVTFESGTDVD
                     LAQVDVQNRLSRVEPRLPEEVRRQGITVNEANSNFLVIVALTSPNGSRSALELGNYAS
                     TQILDELRRVPGVGNVFLFGSEYAMRIWLDPERLAGYNMSAAEALAAVREQNSQTPGG
                     QIGALPSPEGQQLNATIVTQSRFTTPEQFSQVILRANTDGSTVRLGDVARVELGASNY
                     DFTTRLNKQPMAGIAIQLASGANALEAGQGVKDRMEALKAFFPPDTAYSIPYDTTLFV
                     SISIEEVLITLAEAMVLVFGVMFLFLQSFRATIIPTIVVPVALLGACLGLYFLDYSIN
                     VLTLFGMVLAIGILVDDAIVVIENVERIMAEEGLPPREATYKAMGQIVSPIIGITLVL
                     IAVFVPMAFFPGSTGGIYRQFSVTLAFSMAFSALLALTLTPALCATLLRQSHHTTEGR
                     GFFARFNRGFARTTNRYEKGVAALLSRPKRWLAVYAALVAIVGAIYLSVPTAFLPEED
                     QGFIIAVVQAPAGATRERTQLAVEEAEDFFMAQPQVRQVISVLGFSFFGSGQNAAIMF
                     VPLKPWDERGGAENSAAALAGRSMGALGGSKHAMIFALSPPAIQELGNSSGFNMRLQD
                     RAGLGYQKLVDARNQLLGSASQSTEVVGVRPEGLEEGPQLRVSIDRIKARALGLRIED
                     VNNTLTIAFGSAYANDFNREGRVLRVVLQADAAQRMTPEDILQLRVPNAEGGMVPFSA
                     FTSAEWTAGSPQLQRYNGFPSMTVSGSAAPGKATGDAMSEMARLAAQLPQGIGFEWSG
                     LSFEEQQGAGQVPLLLALSLLVVFLLLAALYESWSIPIAVLLVVPLGVLGALLFGLVR
                     GMPMDVYFNIGIITIIGLSAKNAILIVEYAKDLEDSGRGVVDATLEAVRLRFRPILMT
                     SLAFILGVLPLVISSGAGAAGRRAIGTGVMGGMITATALGLFFIPLFFVVIRGLLNRR
                     AARRPKPGPTEGLAHA"
     gene            complement(17265..18422)
                     /locus_tag="VF589_12860"
     CDS             complement(17265..18422)
                     /locus_tag="VF589_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8258514.1"
                     /translation="MPGRTLSLAALSALSLLAAGCSSGQGAPPAPPPAEVSFITVAPT
                     PVVNLVELPGRVAAVRTAEVRARVDGIVEQRLYTEGSDVGRGAALFRIDSRPLRATLD
                     VQLAAVRRAQAEADNAQREVTRFRPLVARDAISKQEFDAAIARLAQARAEVGSAQAQV
                     RQAQLTLGYTTVTAPIAGRVGRAEVTEGALASQAEGTLLTRIEQLDPIYVNFSQSSDE
                     LLALRRGAQGGGLARTVTLLLEDGSEYGVTGTLNFLDQAVDPTTGSVSLRASFRNPQR
                     LLLPGQFVRVRIEAGANASGIAVPQRAVQLSGGGANVMVLGPGNVAAPRPVKLGRLAG
                     GSWVVTEGLKIGDKVIVDGLQKVQPGAPVKPVPLRAAAAPAAPGGSRANAR"
     gene            18626..19183
                     /locus_tag="VF589_12865"
     CDS             18626..19183
                     /locus_tag="VF589_12865"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8258515.1"
                     /translation="MLSAAHDLFAEKGYEATTLSDVVGRSGGSLATLYDMFENKPGLL
                     RAIVTERCSVVSEAIDRAVSAHRPAREALREIAEHMFDKIVDPNAAALFKAALAQPDL
                     GRQLYEAGPATGQAKVAEYLQAQAREGNLSIEDPAEAAQHFFQIMFGHLQTRQMFGLE
                     IKLSEADKARHIDRALNAFLKIYGG"
     gene            complement(19324..19854)
                     /gene="ssb"
                     /locus_tag="VF589_12870"
     CDS             complement(19324..19854)
                     /gene="ssb"
                     /locus_tag="VF589_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8258516.1"
                     /translation="MAGSVNKVILVGNLGNDPESRSFANGGEVVNMSVATSESWKDRD
                     GNKQERTEWHRVVIFNENLGRVAKQYLRKGSKVYLEGQLQTRKWQDNSGQDKYSTEVV
                     LQRFRGELVLLDSKGGGGGAYGGGGDDYGDSFGGGAGGSGGGAGGGSSFGGGQRPQSR
                     PTAPAFDSDLDDDVPF"
     gene            complement(19864..21723)
                     /locus_tag="VF589_12875"
     CDS             complement(19864..21723)
                     /locus_tag="VF589_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX8258517.1"
                     /translation="MTSIPLVAVVGNPNAGKSALFNMLTGARQKVGNYPGVTVERKSG
                     RLALADGRPIELVDLPGAYSLDPSSPDEEVTRDVVLGRQAGERLPDALLLVLDATNLD
                     NHLRFALQLIALGLPIVIALNMVDLARRDGLVIDPQTLERELGVPVVETVAVRRRGGD
                     ALKEALSEAVGAARRLRPGDGAEQEDFVAIQRRARAIARLATLSETSERRTTSIIDRI
                     ALHPVAGPLIFASIMFLMFQAVFAWSEAPIRWIEGLVSGAQGLVVDALPDNFLRSLLV
                     DGVLGGVGAVIVFLPQILILFLFILLLEATGYMVRAAFLMDRLMARVGLSGHAFIPLL
                     SSFACAVPGIMATRTIEDPKHRITTILVAPLMTCSARLPVYAVIIGAFIPATSVGYGI
                     GLQGLVLFGLYLAGIIGALIAATVLRSTVARGVHQGFTLEMPKYQLPSLRDIAIGLWQ
                     RALIFLRRAGTIILVSTIVLWALLSFPQPEEGSGQTVLDTSIAGRIADGLEVVVAPIG
                     FNKEIALALVPAMAAREVAVSALATAYSIEADDEAATETSLIERLRGRWTLPTALAFL
                     AWFVFAPQCLSTVAVTRRETNGWKWPLFMLGYLFTLAYLAAGATYWAATALGL"
     gene            complement(21720..21977)
                     /locus_tag="VF589_12880"
     CDS             complement(21720..21977)
                     /locus_tag="VF589_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX8258518.1"
                     /translation="MTLDQLPFRIPATVAAIDWRALSDGDARRLRNLGFDEGVEIEAL
                     HGAPFGKDPLAVRIGRMMVAMRRAQARAVTVAVAELEIAAE"
     gene            complement(22125..23708)
                     /locus_tag="VF589_12885"
     CDS             complement(22125..23708)
                     /locus_tag="VF589_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258519.1"
                     /translation="MPILTVGPNSTFPSIADAMAAAGPNDTIQLEGGYSNETATVTHS
                     GMIVAGEANSTGIVLRLATGIAVVTLAGNAPIDLFDAADGNAITGNDGDNVVRVSAGS
                     DAVSGGGGHDRLVVDYRLATGAVTGDSASNFTEAGGGARMVTVNGGFEDFTIVTGSGA
                     DTITTGDGDDIIRTHGGAGTITAGQGANYIVGGDGADTITASDGGNFVRGGDGENTIT
                     TGGGSDEIVTGADADTVVSGGGADRITVVGGADSVDAGAGDDRLVLDYSAAITSVSGG
                     VESGNAATGYTGHIADLAGATLDFVGVESFRIMTGAGDDSIRTGAGADLFYGGDGNDR
                     FETGQNDDRAYGEAGDDWVAASGGDDLLDGGAGRDLLYGEGGLDLLVGGGGDDRLAGG
                     SDADKLLGGNGNDTIIGGTGRDGINGGTGSDQFVFAAGDTGTTALTADRIVDFSHADG
                     DKIHLGQIDADTRTAADDAFTFIGTGAFAGNGAAGQLRYDVIGGNRFIEGDVDGDGAA
                     DFSIRLDGAQHLVSTDFIL"
     gene            complement(24049..>25095)
                     /locus_tag="VF589_12890"
     CDS             complement(24049..>25095)
                     /locus_tag="VF589_12890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8258520.1"
                     /translation="VLHPTWSPDGRSLRFTVEDDGGEWVARVPAAGGKLAEVAGGRRI
                     FDAIHANAAGKTAVLVSDPATPPEVYALEGGKLRPLSRQNEAWLAGLQLAPVELTRFT
                     STDGTEVHGFLTRPLGAPAGAPHPTLLRIHGGPASQYNHGFSFEWQLFAANGYAVVAA
                     NPRGSTGRGEDYSRAIYAAWGGVDVKDVLAAVDDAVARGIADPNRLGVGGWSYGGMLT
                     NYVIASDTRFKAATSGAGIANILSGYGTDQYIRDYELELGKPWEKPETWMRVSYPFFQ
                     AGRITTPTLFLVGQQDFNVPLLGSEQMYQALKSRGVDTGLVIYPGEYHGLKRPSFVRD
                     RLQRYLDWYGARLK"
BASE COUNT         4029 a         8326 c         8916 g         3824 t
ORIGIN      
        1 cggcgacggc ggggcccttt gcttatgcgg cccttggtca ggcggccctg agcgcgcgcg
       61 tccccgcccc gccggcggct ttcagcagcc gggcgtggag cgacttcaac cgcgccggat
      121 gctcgatctt ctcgcccttg aggtccgtta ggtagaagac gtcgacggcg cgttcgccat
      181 aagtggcgat atgggcggag tggatcgtcg cccgcgcctg cgagatcgcc tgggcgagcg
      241 cgctcagcaa ggcggcgcgg tcgcgggcgt tgacctcgac gaccgtgtag cggctcgacg
      301 cgcggttatc gatgaacacc gcgggcttga cctcgaacgc ctcggcgcgg gcgagcggca
      361 gccccttcgc ctccagccgc tcggtcatcg gctcctggcc gtcggcggcg gcggccaccg
      421 cggcctccag ccgctggagc tggtggggat cgtcgaaggc gcggccgccg ccgtcctgga
      481 ccaggaaatt gtcaagcgcc atgccgtcgc tgctggtgtg gatgcgggcg tcgatgatgt
      541 tgccgccgac caggctgatc gctccggcga cgcggtagaa caggcccggc tggtcgcgcg
      601 catagacggt gagcagggtc gcgccgcgct cttcgtggac gctggtctgc accgggctgg
      661 tcgcggccgg cgcgcggtcg gcgcggtcga tgaagcgggc attctgctcg agtatgtcgg
      721 ccggctcggc cagccagtaa gcgtcgggaa agcgccaggc gagccgggcg aagcggctct
      781 cgtcccagcg cagccgcgcc tgcacctcct cctgcgtcgc cgccacccgc tcgcgccgcc
      841 ccttctgctt gtggccgagc cggagcaatt cctcggcggc ctcgaacaat tcgcgcagca
      901 gctggcgctt ccagctgttc cacacgccgg ggccgacggc gcggatgtcg accacggtca
      961 ggatcagcag cagccggagc cgttcgaggc tggtcacctc ggcgacgaaa tcggagatgg
     1021 tcttggggtc ggacaggtcg cgcttgaagg cggtcgcgga catgatgagg tggcggcgga
     1081 ccagccaggc gacggtgtcg gtctcggctt cgtcgagccc gaagcgcggg cacagctcgc
     1141 gcgccacctc ggcgccgagc aggctatggt cgccgccgcg gcccttggcg atgtcgtgga
     1201 gcagcaccgc ggcgtagagc acccggcgcg aggagatctg gtggatgacc gcgctcgaca
     1261 gcggatgctc gtcgcgaagc tcgccgcgct cgatccgcga gagcagcccg atcgcccgca
     1321 gcgaatgctc gtcgaccgta taatgatggt acatgtcgaa ctgcatctta gccacgacgc
     1381 ggccgaaatc ggggacgaag cggccgaaga tgctggcctc gttcatccag cgcagcaccg
     1441 tctccggatc gcgcggcgag gtcagcacat ccaggaacaa agcgttggcg cgcgggtcgg
     1501 cgcggacgcc ggcgtcgatc agccgcgcgt cgcggccggc ggcgcgcatc gccagcgggt
     1561 ggatttcgag gccatatttg tcggccagcg cgaagatctg aatgagccgc accgggtcgt
     1621 cggcgaagaa atcgtcgctc ggcagcgcca gccggccgcg gtcgagcagg aagccttcga
     1681 gcttgcgcgg gcgccagcgt atcgccggca gcccgaaccg gcggccggtc ttggcgaagc
     1741 gctcgtcgag atgggcgagg aacaggccgg tgaggtcgcc gacggctttg gcgtgcagga
     1801 aatagtggcg catgaaccgc tcgaccggcg cgttgccggg ccggtcggca taatgcatgc
     1861 gcagcgccac ctcgcgctga tagtcgaggc tcagccggtc ctcggcgcgg ccggcgatgc
     1921 aatgaaggtg gcagcgcacc gcccacagga agcgctcggc cttctggaac cggcccagct
     1981 cctcggcgga aagcaggccg gtctcgacca gttcgggcac cgagcgcacg cgataggcat
     2041 atttgccgat ccagaacaac gtgtgcaggt cgcgcagccc gcccttgcct tccttgacgt
     2101 tgggctcgac gacgtagcgg ctgtcgccca tccgggcgtg gcgggcgttg cgctcggcca
     2161 gcttctcggt gacgaagagg tcgaccttgc cggccacgac ttcgccccag aagcgcgcct
     2221 ggacctcatc gtacaaggcc tcgtcgcccc acagaaagcg cgattcgaga atggcggtgc
     2281 ggatggtatg gtcgctgccg gcgaggcgga cgaggtcgtc gaccgaacgg ctcgaatggc
     2341 cgagcttcag gcccaggtcc cagagcagat agagcatcgc ttcgatgacc tgttcggtcc
     2401 agccggtcgg cttccacggc gtcacgaagg cgaggtcgac gtcggaatgg agcgccatct
     2461 cgccgcgccc gtagccgccc accgccatca gcagcagccg ctccgaggtg gtcggggtgc
     2521 cgagcgggta gagctgcccg gtgacgaaat catggaccag ccgcagcaat tggtcggtga
     2581 ggaaggcgta agcggccgcg atctcgctcc ccgcataagg cttttcctcg agccggcggg
     2641 cgatctcccc gcgcccgtcg gcgagcgcct gcttcagcat cgatttcgcg gcgagcttcc
     2701 tgccctgctc gggcagcgcg cgaagttcct ccagccgctc ggccagcacg cgccgatcga
     2761 tgatcgaacg gcggctcggg atcgggtggg ggcggtgcgc catggcccca gtctaacgtc
     2821 taatcggcgt taccgacacc gcccgcccaa agcgaaagcc ggggaccggt tcattcctcg
     2881 gcggcgaggc gcttcagccg gtagaccagc tccagcgcct cgcgtgggct gagcgcatcg
     2941 gcgtcgattt ccgccagcgc cgcctggagc ggatcgggcg cggcctcctc ctgtgcggcc
     3001 gcggcggcga acagcggcag gtcgtcgagc cccgcggcga tgccgccggt ggcggcgcgg
     3061 ccttcctcga gcttggcgag caccgacttc gcgcgcgcca gcaccggcgg cggcaggccg
     3121 gccagcttgg cgacggcaag gccgtaactg cggtcggcgg ggcccttggc gagctcgtgc
     3181 aggaagacga ggtcgccctt ccattcgcgc gcgcggacat ggtggagcga cagcgcgtcg
     3241 agccgctcgg cgagccgggt gagctcgtga taatgggtcg cgaacaggca gcggcagcga
     3301 ttgcggtcgt ggatcgcctc gaccaccgcc caggcgatgg cgagcccatc ataggtcgaa
     3361 gtgccgcggc cgacctcgtc gaggatgacg aagctgcgct cggtcgcctg ggcgacgatg
     3421 gcggcagttt cgaccatttc gaccatgaag gtggagcggc cgcgggcgag attgtccgac
     3481 gcgccgacac ggctgaacag gcggtcgacc agccccaaag tggcgcggga ggcgggcacg
     3541 taggagccgg cctgggcgag caggacgatg agcgcgttct ggcggaggaa ggtcgacttg
     3601 ccgcccatgt tggggccggt caccagccac agccgctcgc cttccgagag ccggcaatca
     3661 ttggcgacga agcggccgcc cgcggttcgc accgcttcct cgaccaccgg gtgacggccg
     3721 ccctcgatgt cgaagcagac atggccggca aattggggcc ggcaccagcc gccctcggcg
     3781 gcgcgctcgg cgagcgcggc ggcaacgtcg agccgcgcga gcgcatccgc cgcggcggcg
     3841 atcgggtggg cgcgggcgag cgcggcggcg gtcagctcct cgagatgggc ggcctcggcg
     3901 gcgagcgcat gggcgccggc ctggccgacg cgcaccgcct ggtcgtggag gtcgggcgcg
     3961 ttgaagcgca ccaccccagc cagcgtctgg cggtgggtga agcccgattc cgccgccatc
     4021 agccggtcgg cattcctggc cggcacctcg atatgatagc cgagcacgcc attgtggcgg
     4081 atcttgagcg cggcgatgcc ggtctgctgg cggtagcgcg cctccagcgc cgcgatcgcc
     4141 cgccgccctt cgccgccggt ggcgcgcaga tcgtcgagcg cggcatcata gccctcggcg
     4201 atataaccgc cctgcgcggc atcgaccggc ggcatgggca cgagcgcgcg cgcgagcagg
     4261 tcgaccagct cgccatggcc ggtcagcgct ggcagcagct ccagcagcat cgccggaaga
     4321 tcgggcaagg cggcgagccg gtcgtgcagc cggcgcgcct cgtcgagccc gtcgcgaagc
     4381 tggccgagat cgcgcggcga gcccctgccg gcagcgatcc ggccgagcgc gcggctgacg
     4441 tccggaagcg ccttgagctg ccgccgcagc cgatcgcgaa gcccggcatc ggcttcgaac
     4501 aggccgacga gatcgagccg cgcctcaatc gccggccggt ccagcaaagg cgcgccgaga
     4561 tcggcggcga gcaggcgggc gccggcgccg gtgacggtcc ggtcgaccgt cgccagcagg
     4621 cttccggcgc gggtgccgct cgccgacacc gtgagctcga ggctttcccg ggtggcggcg
     4681 tcgatcatca tgtgccggcc ctgcgcgcgc ggctcgggcg ggcgcaggaa ggggagcgat
     4741 tcgcgcgcga cctcgtcgag ataagcgagc agcccgccgc ccgcggccag cgccgcccgg
     4801 tcgaaggcgc cgaagccatc gagggtggcg acgccgaacc tctccttgag ccgtcgctcc
     4861 gcctggacgc tgtcgaaccc cgcgcgccgc agctgcaccg gctggccggg cagcggctcg
     4921 cacgccacca attccgccgc cgccaaccgg gcgagctcgg catcgagcgc cgccggcgcg
     4981 accgagaaca gttcgaaacg acccgtcgag atatcggcgg cggcgatgcc gtagcgatcg
     5041 ccgaccttcg ccaccgctgc gagccagttg gcggcgcggg cgtcgagcag ggcttcctcg
     5101 gtgagcgtgc ccgcggtgac gacgcgcacg atcgcccggc tcaccaccga cttgtggccg
     5161 cgcttcttcg cttcggccgg gctttcggtc tgctcggcga tggcgacgcg gtggccggcc
     5221 ctgatcaggc gcgcgagata ggcctcggcg gcgtggatgg ggacgccgca catcgggatc
     5281 ggctggccct catgctcgcc gcgcgcggtg agagcgatat cgaggcaggc cgccgccgcc
     5341 ctggcgtcgt cgaagaacag ttcgaagaag tcgcccatgc ggtagaaaag caggcaatcg
     5401 gatgcctccg ccttgatcgc gagatattgc gccatcatcg gagagggtgc ggagatcggc
     5461 tttgcctggg tggccatctc cgcaagggta aagagtcgga cggcaaagcg gaatgggaaa
     5521 gggcgatttc tccccaggtt cctgcctgcg cgggccgccg cggctgtcac agacggatcg
     5581 cggcaaagcg acgagcgacc ggtcccacct ccgaacggcg tcggcccggt cgctctcgcg
     5641 ccggcagcgt cagcccggcc ttaccggctt cggccgcacg ttcctcggag cggtcagctg
     5701 gaacgtcatt ccaccgaccg ggatgccttc ctgcaggacg gtcaccacga tgttccggtc
     5761 ccgctgccgg gcgaaatcct cgggagcgat cggccgccct ttcttggctt cggcgagggt
     5821 caccttcgcc atctcgccgg gctttatcac aagcttcctg cgcgccgcct ggggcacgtt
     5881 gaaggcccag ccgagcttcg ctagccaggc aggcagcttc acctcgacgg tggtgcgaac
     5941 cgccttgcgg ccgtgattgc ggatccagaa gggaagccgt tcccattcga tcccccgctg
     6001 cacccccgtc aggggagctt ccactggatg cacgttcctc tggcccaggt tgttgtcgtg
     6061 gggaaccagt cgccattccg gaatcggtcc gacgacccgg ccgtcgatat tgcctgcgtc
     6121 gccctgcgcc gagacgctga agaacatgca ttcatgcccc acctgcgacg gcgtccactg
     6181 gaacggcccg acgacgacgc ccgccatgtc gcctgcggcg acgttcggcg cggcaagctg
     6241 tgccgtcgtc atcggcgccc agtcgccggg gaaggccagg cccgcgccgg gaagcgcgtg
     6301 gaaacccttc accaccacgt tcgtggcggg ctggctgccc cgattcttta tccgcacata
     6361 ggcatagttg gtctgcccga cgatcggctc ctcgtgcagg ccgccgcccg ttccgggcgc
     6421 cgtgcggttc cagatgtcgc cgcaactcca gtgattgggc tggtacggat attcgccgtt
     6481 cctgccgtcg tctatgaaca catccactga aggcgccacg ccttccacgg tggccggcgc
     6541 gcccggagcc cggaacagcc cctgcttctc gaacgcccag cggatcacct tgtgataggc
     6601 gccgccggcg taggcgccag gcgggctggt cgagttccag acgccgacat cggccgtctc
     6661 gagctggccc tcccaagcca gcggcgaagg cgggttcgtc accggcgtca gctgcccaac
     6721 ggcccgcagg atcaggtaga cggtgctgcg cgcggcgaag tccttggccg cgggcgcagc
     6781 tgcgtcgccg cccagcgagc ggtagatccg gaaatgcgtg gtggcgagga tctgctcgct
     6841 cgaatagccc ccgacgtcgt tcgcgccgcc ccagccccag ccattgaccg gccggtcgtg
     6901 acgtcggccg atgttgaccc acgggaatgt gacgaagcgg tcgggcgccg ccgagccagg
     6961 atcgttgagg atcgcggcga tgctgtcgcc agcgctgtgg gcaaacccga aattcgggga
     7021 gttcacgtga tcccaaagga taccgtgacc gccgatctca tggatcacca cccgccaatc
     7081 ggcgccgatg ctgatcgggt tggcgacatc gccagtgtgg gcgagggcga aattcacctg
     7141 gccgatcccg ttcgccatcg cgttcccggg gcaattggcg ttcaccacgt tgccgagacc
     7201 gcgatggtcg actcgaaccg ggaaggcggt gccgtcgaaa tagctcgcga tggagaagcc
     7261 gagatcgccg accagctgaa agaagcggtt gcagtgatag taagcgttcg ccgccgcgaa
     7321 atcgttcgtc cgggatacgt aatcgaagtt gaacggagtg actcgcgtcg gcgcgccgat
     7381 cgccggcgcg gagttgtcga agagtcgcac atgggttccg ctcagcgatt gggtcccgcc
     7441 cacggccggc gacagattgc tgagcgtcac gttgtcgcgc agcgcatcca gcgccgccgc
     7501 gctcgcactc ggcagattgg ccgcgaggcc tgttttggtg atcggatccc gctcgaagac
     7561 cagcgcgctg gcacaatcca ccagggcccg caggtagaga acggcgccgc tgtccacttc
     7621 gatgaaggca cgccagttca tcgtacccca cgccttgtcg gggagcgtga acagcgcatc
     7681 gacgacgata taatgtgtgc cgtcggcgat tgcggcaggc gctggttcga gcgggaggga
     7741 cgggatgccg ccgcagaagc cgagaccgtg cttgtcgtga ggatgcgact cgagccgcct
     7801 ttcggcatcg taccgataga ccatcagacg agtgccgttg attcgcgcac ggggtccgaa
     7861 ttttccgagc gcgcgccgca ccaggtcgtc gtaagcgccg ttgcttgcat cggggcccga
     7921 agcggtgagg ggcaccagtc ccgaagcagg ggcgggcgcc tcgatgtcat agtggagcgt
     7981 cgacgacgcg gccgcgactt cattcgccgg gccttgcgtc gtgacggata ctcccgcctc
     8041 atggatcgga agcccgaaca tggtctggac ataggtgacg gttgtcgtgt ccatgatcct
     8101 tttttcgctc tcgaatcgca gcgcctgccc ctcgggcgaa ggtgcgattc cagcactcag
     8161 ctcaaggctc gcagctcctt ccggggcgat gccgagcgtg gccgcattcg ccagcaggaa
     8221 gttgcgtgcg ctttcctgtg cggtggcgcc ggcacggccg gtctcagccg cgagcgccat
     8281 cgcctcgatt gcgtctgcag gttcgctgcg gtcgaacttg aaatagcgcg cgtgcccgcc
     8341 ttcgtcctga acgacgtttt gttgatcagc catgatggaa ctactccaga gagtgccatc
     8401 tctgttgtaa gccagagtcc cgcgagcagc aacatacttg gtcctatttg gacatagtga
     8461 gtggattgca tggaagttac taatgttgct gagagattta cggagtgatc aacggcccgg
     8521 aggaggttgg actccaactc ggaccgatct cttgttcgca aattctgtgt gttcgcgtcc
     8581 gggggaccgc cacggccggt cagtagcgcg actcggccgg agcactgcag ggaaacccca
     8641 cgggcggaac attccctcgc cggtggcgct tcgggtctta cctccccgga gtgcttgctc
     8701 ccgtcgagtt cgacgttcta gaggcggtcg ttccgtgcgc gagataaaga gagcccgtca
     8761 tgagcgagag caacgtcaaa ttctcggaag ccgaagcact ggagttccac tcccgcggcc
     8821 ggccgggcaa gatcgagatc atcgcgtcga agccgatggc gacccagcgc gacctcagcc
     8881 tcgcttattc tcccggagtc gcggtcccgg ttcgcgccat cgccggggat cccgccaagg
     8941 cctatgatta tacctcgaag ggcaacctcg tcgccgtcat ttccaacggc accgccattc
     9001 tcggcctcgg caatctcggc gctttggcct cgaagccggt gatggaaggc aaggcggtgc
     9061 tgttcaagcg cttcgccgac gtcgattcga tcgacatcga ggtcgacacc gaggacacgg
     9121 acgccttcat caacgcggtc gccttgctcg aacccacctt cggcggcatc aaccttgaag
     9181 atatcggcgc gccggcctgc ttcatcatag agcaggcgct gaaggagcgg atgaacatcc
     9241 cggtgttcca cgacgaccag cacggaaccg ccatcatcac cgccgccggc ctgatcaatg
     9301 cctgcctgct gaccggccgc gacttcaagg acgtgagggt ggtggtgaac ggcgccggcg
     9361 ccgccgccat cgcctgcacc gagctcatca agtcgatggg cgtgcgccac gaccatgtca
     9421 tcatgtgcga ccgcaagggt gtgatctggc agggccggcc ggacggaatg gaccagtgga
     9481 agtccgccca tgccgccgtc accgacgccc gcacgctcca cgaggccctg gtcggggccg
     9541 acgtgttcct tggcctgtcg gcggccggcg cgctgaagcc cgaaatggtg atgaagatgg
     9601 cggagcggcc gatcatcttc gccatggcca atccggaccc ggagatttcg ccgcccgacg
     9661 cccgcgaggc gcggcctgac gcgatcatcg ccaccggccg ctcggactat ccgatccagg
     9721 tcaacaacgt gctcggcttc cccttcatct tccggggcgc gctcgacgtg cgcgcgacct
     9781 cgatcaacga cgagatgaag atcgccgcgg ccgaggcgat cgccgagctc gcccgccagc
     9841 aggtgcccga ggaagtcgcc gccgcctatg gcggccgctc gcacagcttc ggccccgatt
     9901 acatcatccc cgccccgttc gatccgcggc tgatggagct ggtgccggcg gcggtggcgg
     9961 cggcggcgat gcggagcggc gtggcgcgct tcccgatcaa ggacatgacc gcctatcgca
    10021 ccgcgctgcg atcgaggctc aatcccacca cctcggtcct gagcctcgcc tatgaagcgg
    10081 cgcgggccaa cccgaagcgg gtgatcttcg ccgagggcga ggaggagacg gtgctgcgcg
    10141 ccgccatcgc ctttcgcgag ggcggctacg gcattccggt gctggtcggg cgcgacgacg
    10201 tctacgaccg gctgcgcgcg ctgggcgtcg acgatcccga ttcgttcgag atccagaaca
    10261 gccgccgctc cgagcgcatc ccggaaatgg tcgactatct ttaccggcgg ctccaccggc
    10321 gcggctatct gcaccgcgaa gtcgagcgga tggtgaacca ggaccgcaac acgttcggcg
    10381 cgctgctgct cgcgctcgga gagggcgacg tcatgatcac cggcgtcacc cgcccctacg
    10441 gccagagcat ccgccagatc cgccgtgtgc tcgatcccaa ggagggcgtg accccgttcg
    10501 gcattcacgt cctcgtcggc cagagccaca ccatcttcat ggccgacacc accgtcaccg
    10561 agcgcccgac ggccgagcag ctcgcccata tcgcctgcga gaccgccgcc gtggctcgcc
    10621 ggatgggcca tgagccgcgg gtggcgttcc tgtcctattc gaccttcggt aatcccgaag
    10681 gcaccttcct cgacaatgtc cgcggcgcgg tgcggctgct cgacggccgc agcgtcgatt
    10741 tcgaatatga aggcgagatg gcgcccgacg tggcgctgaa cccggcgctg aagccgatct
    10801 atccgttcag ccgccttacg ggcccggcca acgtgctgat catgccgggc ctccagtcgg
    10861 cgaacctctc cgccaagctg ctcgagcaac tgggcggaga cgcggtgatc gggccgatgc
    10921 tggtcggcat ggcccatccg gtccaggtcg cggcaatgac ggcgagcgcg tcggacctgg
    10981 tgacgctggc ggtgctcgcc gccggcggca tcgtccgctg atcctcagct ggcggtgaag
    11041 gccttattgc ggattgtcgg cgctgcccgc cggcgccgcg gtcgacacct ggccgatgct
    11101 tccgaacagc tcttcgaaga agctgcggtc gcggccgagg gtcggcgtct tgtcgttcat
    11161 cggatcgatc gaggcgacgc gctcgaagcc cgagcggtcg accgccacca cgttgccggc
    11221 ttcatcgaag cggatgtgga gcacggtatg ctcgaccgcg cggggatggt tgaacgccat
    11281 ttgcagcgtg cggcgcgaca cataatacca gtcgcgctgg tcgaactgac cgacgaaggt
    11341 gggccggccg agcgtgccct ggacggattc gcgggtgtcg acgccgggct ggatcgcgct
    11401 caccagcact tcgtcgatca gataaccctg gcggccgcgc accttctggc aggcgctggt
    11461 gagcagggcc gcgccgatca gcatcgcggc tgcgaggctt cgtccgtggc ggagggtcat
    11521 cgaaatcttc tcctgaaaat cagctggagc ggcacggcgc accccgcccc tgcttgcggg
    11581 cggccgttcc agcccccaat atccagtaac tggccacagg cgcaagagcg cgccgcagac
    11641 ccagagggat agtttgtcgt tcctggcgcg catcttcggc gagaaagagg accgggaggc
    11701 gctcgggccg ctctatgcgg cggtcgtcgc cgctggccgc gacccggcct ggttccggca
    11761 ggggcaggtg cccgacacgc tcgacgggcg cttcgacatg atcgccgcct tgctgaccct
    11821 ggtgctgttg cgcctcgagg cggacggcga cagcgggcgc cgcgcctcgg tggtgctgac
    11881 cgagcgcttc atcgacgaca tggacgggac ggtgcggcaa atgggcatag gcgacctgat
    11941 ggtcggcaag catgtcggcc ggatgatgag cgcgctcggc ggccgcctca ccgctttccg
    12001 cgccgccggg cagggcgacc tcggggagcc ggtcgcccgc aacgtgtttc gcgacgcggt
    12061 gccgtcgccg gaagcgttgg cgttcgtcag cggccggctg gagcggtatc gcgacgcgct
    12121 tcaggcgacg ccctcgcagg cgctgctcga cggggagatg ccctcgctat gagcccggaa
    12181 ctcagccgcc cggtacgggt ggacacgctt ggcgccacgc cgcgcagcct gtcggtggag
    12241 gccgatgcgc gggaacgggc ggcgctcgcc gagcggttcg gaatgccggg gatcgacagc
    12301 ctctccgcgg acgtcgagct caccctgaag ggcgaagcgg tgatcgtggc cgggacgctg
    12361 aaagcatccg tcacccagag ctgcgtcgcg accggcgtcc ccgtccccga gcgggtcgac
    12421 gagccgttca cggtgctgct gaggccgcgg ccggaggccg gcgcggaaga ggagatcgag
    12481 ctcagcgaag gcgagctcga cgtggtcttc tacgagggcg ctgccgtcga tgtcggggaa
    12541 gtggtggcgg aaacgctttc gctggcgctg gagccttatc cgcgctcccc ggaggcggcg
    12601 gcggcgctca aggaagccgg cgtgctgagc gaggaggaag ccaagcccgc cagcccgctc
    12661 gccggcctca aggacctgct cacccccaaa tcataaggcc ccaaatcatg agggcccaaa
    12721 tcatgacggc gcccgaagcg gcgccgagat cggcgccgca ccctcgatgc cgccgccgag
    12781 cgcgacgtag agatcgaccg cattggtgag ctgctcgcgg cgggtggcca gcacctgctg
    12841 ctcggccgcg aacaactcgc gctgggcgtc gagcacttcg agatagccgg tgacgccgtt
    12901 ctgatagcgc aaggtggcga gctcggcgcg gtcgcgctgg gcggcgagct cgcgctgctg
    12961 cgcggtcagc cgctcggcca gccagcgccg ctcggcgagc cggtcggcga cttcgcggaa
    13021 cgcctgctgg atcgcgcgct cgtaggaggc gacggcaatc ccctggcggg cgaccgcgac
    13081 gtcgagattg ccgcgggttc gcccggcgtc gaagatcggc aggctggcgt tgccgccgag
    13141 gctccaggtg aaggcgccgc cgctgaacag gctgccgagc gcgccgctgg cgaagccgag
    13201 cgccccggtg aggctgatct gcgggaagaa ggcggcgcgg gcggcgccga catcggcata
    13261 agcggcgcgc agcagctctt cggcctccaa tatgtcgggg cggttggtga gcagctccga
    13321 cggcaggccg gccgagatgg tctcgacgat gccctgtcgt tccagcggca gcggcggcgg
    13381 cagcggcgcg cccaccgggc cgccgacgag gaattcgagc gcattgcggt tctgcgcgcg
    13441 ctgcaattgg aggtcggcca gcgcggcctg ggcctgggtg agcagggtct cgatctggcg
    13501 gtagtcgagc gcggaggtga cgccggcgtc gagccgcagc ttgccgatct cgagcgcgcg
    13561 ctcgcggtcg cgcacggtcc gctccgcaat ggcgatgcgg tcgtccagtt cgcgctcggt
    13621 cagataggcc tcggcgacat cggcgaccag gctgatctgg aaggcgcgct gggcgtggat
    13681 ggtcgacagg tagttggcgc gcgccgattc ggtgaggctg cggacgcggc cccagaaatc
    13741 gagctcgaac gaggcgacgc tggcgccgat ctcgaaccgg ttgccggtga cggcgccgcc
    13801 gaagccgtcg ccggtgcggg agcgcgccgc gccggcattg gcgtcgagcg tcggcagccg
    13861 gtcggcgcgg cggatgcgat attggccgcg cgcctcctcg atccggagca ccgccgtcct
    13921 gagctcgcgg ttatggatca gcgcgcgttc gatcagcgcc tgcagccgcg gatcccggaa
    13981 gaagccgcgc caggggagct ccggcgcgcg cggcccggcg gtgacgggcc aggcgtcggc
    14041 cggatattgc ggcgccgtcg gcaggccggg ctggacatat ttgggctgct gggagcagcc
    14101 ggcgagcagc aaggcagcga ggagggggag acggttaggc atgggcgaga ccttcggtcg
    14161 ggcccggctt ggggcgccgc gcggcgcgcc ggttgagcag gccgcggatg acgacgaaga
    14221 agagcgggat gaagaacagg ccgagcgcgg tggccgtgat catgccgccc atcacgccgg
    14281 tgccgatcgc gcgccggccc gccgcgccgg cgccgctgct gatcaccagc ggcagcacgc
    14341 cgaggatgaa ggccagcgag gtcatcagga tcgggcggaa gcggagccgc accgcctcga
    14401 gcgtcgcgtc gaccacgccg cgccccgaat cctcgaggtc cttggcatat tcgacgatca
    14461 ggatcgcgtt cttggcggaa aggccgatga tggtgatgat gccgatgttg aaataaacgt
    14521 ccattggcat gccgcggacg aggccgaaca gcagcgcgcc gagcacgccg agcggcacca
    14581 ccagcagcac cgcgatcggg atcgaccagc tttcgtaaag cgcggccagc aacaggaaga
    14641 cgaccagcag cgacagagcg agcagcaacg gcacctgccc cgccccctgt tgctcctcga
    14701 acgacaggcc cgaccattcg aacccgatcc cctgcgggag ctgggccgcg agccgggcca
    14761 tctccgacat ggcgtcgccc gtcgccttgc cgggcgcggc cgagccggag acggtcatcg
    14821 acgggaagcc gttatagcgc tggagctgcg gcgatccggc ggtccattcg gccgaggtga
    14881 aggccgagaa cggcaccata ccgccctccg cattggggac ccggagctgg aggatgtcct
    14941 ccggcgtcat gcgctgcgcg gcgtcggcct gcagcaccac ccgcaggacg cggccctcgc
    15001 ggttgaagtc gttggcatag gccgagccga aggcgatcgt tagcgtgttg ttgacgtcct
    15061 caatgcgcaa gccgagcgcg cgcgccttga tccggtcgat cgagacgcgc agctgcgggc
    15121 cctcctcgag cccctccggc cgcaccccga ccacctcagt gctttgcgag gccgagccga
    15181 gcagctggtt gcgggcgtcg accagctttt ggtagccgag cccggcgcgg tcctgcaggc
    15241 gcatgttgaa gccgctcgaa ttgccgagct cctgaatggc gggcgggctc agggcgaaga
    15301 tcatcgcgtg cttggagccg ccgagcgcgc ccatcgaccg cccggcaagc gccgcggcgc
    15361 tgttctcggc cccgccgcgc tcgtcccacg gctttaaggg cacgaacatg atcgccgcat
    15421 tctggccgct gccgaagaag ctgaagccga gcaccgagat cacctgccgg acctgcggct
    15481 gggccatgaa gaaatcctcg gcctcctcga cggcgagctg ggtgcgctcg cgcgtcgccc
    15541 cggccggcgc ctgcacgacc gcgatgatga agccctgatc ctcctcgggg aggaaggcgg
    15601 tggggacgga gaggtagatg gcgcccacga tcgcgaccaa agcggcatag acggcgagcc
    15661 agcgcttggg gcgggagagc aaagccgcca cgcccttttc atagcggttg gtggtgcggg
    15721 cgaagccgcg gttgaaccgg gcgaagaagc cgcggccttc ggtggtatgg tgcgactggc
    15781 gcagcagggt ggcgcaaagc gccggcgtca gggtcagcgc gagcagcgcc gagaaggcca
    15841 tcgagaaggc cagggtcacc gagaattggc ggtagatgcc gccggtcgag cccgggaaga
    15901 aggccatcgg cacgaacacg gcgatgagga cgagggtgat gccgatgatc gggctgacga
    15961 tctgccccat cgccttgtag gtggcctcgc gcgggggaag gccttcctcg gccatgatcc
    16021 gctcgacatt ctcgatcacg acgatggcgt cgtcgaccag gatgccgatc gccagcacca
    16081 tcccgaacag ggtcagcacg ttgatcgaat aatcgaggaa atagaggccg aggcaggcgc
    16141 cgagcagggc caccggcacg acgatggtcg ggatgatggt ggcgcggaaa ctctggagga
    16201 acaggaacat gacgccgaac accagcacca tcgcctcggc gagcgtgatc agcacttcct
    16261 cgatcgagat cgagacgaac aaggtggtgt cgtaagggat cgaataagcg gtgtcgggcg
    16321 ggaagaaggc tttcagcgcc tccatccggt ccttgacgcc ctggcccgct tcgagcgcgt
    16381 tggcgcccga ggcgagctgg atggcgatgc ccgccatcgg ctgcttgttg agccgggtcg
    16441 tgaaatcgta gttgctggcg ccgagctcga cccgggcgac gtcgccgagc cggacggtgg
    16501 agccgtcggt attggcgcgc aggatgacct gcgagaattg ctcgggcgtg gtgaagcggc
    16561 tctgggtgac gatcgtggcg ttgagctgct gtccctcggg cgagggcagc gcgccgatct
    16621 ggccgccggg ggtctggctg ttctgctcgc gcaccgccgc caaggcttcg gccgccgaca
    16681 tgttgtagcc ggccagccgc tccggatcga gccagatgcg cattgcatat tctgagccga
    16741 acaggaaaac gttgccgacc ccgggcacgc ggcgcagctc gtccaggatc tgggtgctgg
    16801 cataattgcc gagctccagc gcgctacgcg aaccgttggg cgaggtcagc gcgacgatca
    16861 ccaggaagtt ggaattggct tcgttgaccg taatgccctg gcgccgaacc tcctcgggca
    16921 ggcgcggctc gacccggctc agccggttct gcacgtcgac ctgggcgaga tcgacgtcgg
    16981 tgccgctttc gaaagtgacg gtgatggtgg cggatccgtt cgcggccgag gacgagctca
    17041 tgtagaggaa attctcgacc ccgttcatct cctgctcgat gatcgaggtg acgttctgct
    17101 cgagcgtggc ggcgtcggcc cccggatagg cgacgtcgat cgtcaccgac ggcggcgcga
    17161 tggtgggata ttgctcgatc ggcagcgcgt tgaccgccag caatcccgcc agcgagatgg
    17221 acaaagcgat cacccaggcg aagatcgggc gatcgatgaa gaacctagcg agcattggcg
    17281 cggctcccgc cgggggcggc gggggcggcg gcggcgcgca ggggaaccgg cttcaccggc
    17341 gcccccggct gcaccttctg gaggccgtcg acgatcacct tgtcgccgat cttcagcccc
    17401 tcggtcacca cccacgatcc gccggccagt cggccgagct tgacggggcg cggggcggcg
    17461 acgttgccgg gcccgagcac catcacgttg gcgccgcccc cggacaattg caccgcccgc
    17521 tgcggcaccg cgatgccgct cgcattggcg ccggcctcga tccggacgcg gacgaactgg
    17581 ccgggcagca gcagccgctg cggattgcgg aacgaggccc gcaggctgac gctgccggtg
    17641 gtcggatcga ccgcctggtc gaggaaattg agggtgccgg tgacgccata ttcgctgccg
    17701 tcctcgagca gcagggtgac ggtgcgggcg agtccgccgc cctgcgcgcc gcggcgaagc
    17761 gccaagagct cgtcgctcga ctgggagaaa ttgacgtaga tcggatcgag ctgctcgatc
    17821 cgggtcagca acgtgccctc cgcctggctg gcgagcgcgc cctcggtcac ttcggcgcgg
    17881 ccgacgcggc cggcgatcgg cgcggtgacg gtggtatagc cgagcgtcag ctgggcctgg
    17941 cggacctggg cctgggcgga gccgacctcg gcccgtgcct gcgccagccg ggcgatggcg
    18001 gcgtcgaact cctgcttgct gatcgcgtcg cgcgcgacca gcggccggaa gcgggtgacc
    18061 tcgcgctggg cgttgtccgc ctccgcctgc gcgcggcgaa cggcggcgag ctggacgtcg
    18121 agtgtggccc ggagcgggcg agaatcgatc ctgaacaagg ccgcgccgcg gccgacgtcg
    18181 gagccttcgg tataaagccg ctgctcgacg atgccgtcga cccgggcgcg gacctcggct
    18241 gtgcggaccg cggccacccg gccgggaagc tcgaccaggt tgacgacggg cgtcggcgcg
    18301 accgtaatga agctgacctc ggccggcggc ggagccggag gcgcgccctg cccgctcgag
    18361 cagccggcgg ccagcagcga cagcgccgaa agggcggcga gagaaagggt tcgaccgggc
    18421 atcttcgcgt cctggcagga gaatgaggaa ctggctggag cgactacagg cccccgcgcg
    18481 gcggcgccgc caagggctgg tcaccgcatg aatattagtg taatgtagct tacagtacac
    18541 gtcaaacgga taaaagcggg ccaaaacaat gaactggtgc aaaggcggcg gacgggaaag
    18601 caaacgcgag ctgcggcgac aggcgatgct gagcgcggcc cacgacctgt tcgcagagaa
    18661 aggttacgag gcgacgacgc tcagcgacgt ggtagggcgt tcgggcgggt cgctggcgac
    18721 gctctacgac atgttcgaga acaagcccgg tttgctgcgc gcgatcgtca ccgagcgctg
    18781 ctcggtggtg agcgaggcga tcgaccgcgc cgtttccgcc caccgcccgg cccgcgaggc
    18841 gctgcgcgag atcgccgagc atatgttcga caagatcgtg gaccccaacg ccgccgcctt
    18901 gttcaaggcc gcgctcgccc agccggacct cggccgccag ctctacgagg cggggccggc
    18961 caccgggcag gccaaggtcg ccgaatatct gcaggcgcag gcgcgcgagg gtaatctcag
    19021 catcgaggac cccgccgagg cggcgcagca tttcttccag atcatgttcg gacacctcca
    19081 gacccggcag atgttcgggc tggagatcaa gctgtcagaa gcggacaagg cgcggcatat
    19141 cgaccgcgcg ttgaatgcgt ttttgaagat ctacggcggc tgacgggcgg gggcttggcg
    19201 cctagcctca ccaaagcaac gaattcgtca ttccagcgaa agctggaatc tctttgcctt
    19261 taagaagtga gattccagct tacgctggaa tgaccggctg gctgctgcgc cgacaccgcc
    19321 ccctcagaac ggaacatcgt cgtcgaggtc gctgtcgaag gcgggggcgg tggggcggga
    19381 ctgcgggcgc tggccgccgc cgaagctgga gccgccaccc gcgcccccgc cggagccgcc
    19441 cgcaccgccg ccgaagctgt cgccataatc gtcgccgccg ccgccatagg cgccgccgcc
    19501 gccgccctta ctgtcgagca ggacgagctc gccgcggaaa cgctgcagca ccacttcggt
    19561 cgaatatttg tcctggcccg aattatcctg ccatttgcgg gtctggagct ggccttcgag
    19621 gtaaaccttg gagcctttgc gcagatattg cttggcgacg cggccgagat tctcgttgaa
    19681 gatgacgacg cggtgccatt cggtccgctc ctgcttgttg ccgtcgcggt ccttccagct
    19741 ctccgaggtg gcgaccgaca tgttcaccac ttcgccgcca ttggcgaacg agcgggattc
    19801 ggggtcgttg ccgagattgc cgacgaggat gaccttgttg acgctgcccg ccattttctt
    19861 ctcctagagc ccgagcgccg ttgccgccca gtaagttgcg cccgcggcca gataggccag
    19921 cgtgaacaga tagcctagca tgaagagggg ccacttccag ccattggtct cgcgccgagt
    19981 caccgcaact gtggataaac actggggtgc gaacacgaac caggcgagga aggcgagcgc
    20041 ggtcggcaag gtccagcggc cgcgcagccg ctcaatcagc gaggtctcgg tcgcggcttc
    20101 gtcgtcggcc tcaatcgagt aagcggtggc gagcgccgag accgcgacct cgcgcgccgc
    20161 catcgccgga accaaagcca aagcgatctc cttgttgaag ccgatcggcg cgaccacgac
    20221 ctcgagcccg tcggcgatgc ggccggcgat cgaagtgtcg agcacggtct ggcccgaccc
    20281 ctcctcgggc tgcgggaagg agagcagggc ccagagcacg atcgtcgaga cgaggatgat
    20341 ggtgccggcg cggcggagga agatgagcgc gcgctgccac aggccgatgg cgatgtcgcg
    20401 caggctcggc agctggtatt tcggcatttc gagcgtgaag ccctgatgga ccccgcgcgc
    20461 cacggtgctg cgcagcacgg tggccgcgat cagcgcgccg attatgcccg caagatagag
    20521 gccgaacagc accagcccct ggaggccgat gccgtagccg acgctggtcg ccgggatgaa
    20581 ggcgccgatg atcacggcat agacgggcag ccgcgccgag caggtcatca agggtgcgac
    20641 caggatggtg gtgatgcggt gcttcggatc ctcgatggtt cgcgtcgcca tgatgccggg
    20701 gacggcgcag gcgaaggagg agagcaaggg aatgaaggcg tggccggaaa ggccgacccg
    20761 ggccatcagc cggtccatca ggaaagcggc gcggaccatg tagccggtcg cctccagcag
    20821 caggatgaac aggaacagga tcaggatctg cggcaggaac acgatcaccg cgccgacgcc
    20881 gccgaggacg ccgtcgacca gcagcgagcg caggaaattg tcgggcagcg catcgaccac
    20941 caggccctgg gcgcccgaga ccaggccctc gatccagcgg atcggcgctt cggaccaggc
    21001 gaacaccgcc tggaacatca ggaacatgat cgacgcgaag atgagcggcc cggccaccgg
    21061 gtggagggcg atgcggtcga tgatcgaggt ggtgcggcgt tccgaggtct cggacagggt
    21121 tgccaggcgg gcgatggcgc gggcgcggcg ctggatggcg acgaaatcct cctgctcggc
    21181 gccatcgccc gggcgcagcc ggcgggcggc gccgacggct tcgctgagcg cctccttgag
    21241 cgcgtcgccg ccgcggcggc ggaccgcgac ggtctcgaca accggcaccc caagctcgcg
    21301 ctcgagcgtc tgcggatcga tgacgagccc gtcgcggcgg gcgagatcga ccatgttgag
    21361 cgcgatcacg atcggcaggc cgagcgcgat cagctgcagc gcgaagcgga gatggttgtc
    21421 gaggttggtc gcgtcgagca ccagcagcag cgcgtcgggg agccgctcgc cggcctgccg
    21481 gccgagcacg acgtcgcggg tcacctcctc gtcggggctg gacggatcga ggctgtaggc
    21541 gccgggcagg tcgaccagct cgatcggccg gccgtcggcg agcgccagcc gccccgattt
    21601 ccgctcgacg gtgacgccgg gataattgcc gaccttctgg cgcgcgccgg tcagcatgtt
    21661 gaacagggcg ctcttgccgg cattcgggtt gccgaccacc gccaccaggg gaatgctggt
    21721 cattcggcgg cgatttcgag ctcggccacc gccacggtca cggcccgcgc ctgcgcgcgc
    21781 cgcatcgcca ccatcatccg cccgatccgc accgccagcg gatccttgcc gaagggcgcg
    21841 ccgtgcagcg cctcgatctc caccccctcg tcgaagccga gattgcgcag ccgccgggcg
    21901 tcgccgtcgc tgagcgcgcg ccagtcgatc gcggcgacgg tcgcgggaat tcggaaggga
    21961 agctggtcga gcgtcatcga gtccacatcg tctagatgcg actgattatc aataacgagg
    22021 ccggaaggcc agtgcggatt gcagctccgg ccgccgccct gcgccgcaca aacggacggg
    22081 gacgccgcgg ccggagccgc gacgtccccg cgaagagcct tggcctagag gatgaagtcg
    22141 gtgctcacca ggtgctgcgc cccatcgagc cggatcgaga aatcggcggc gccgtcgccg
    22201 tcgacatcgc cctcgatgaa gcggttgccg ccgatcacgt cgtagcgcaa ctgtccggcc
    22261 gcgccgttgc cggcaaaggc gccggtgccg atgaaggtga aagcgtcgtc ggcggcggtc
    22321 cgggtatcgg cgtcgatctg gccgaggtgg atcttgtcgc cgtcggcatg gctgaagtcg
    22381 acgatgcggt cggccgtcag cgcggtggtg ccggtgtcgc cggccgcgaa cacgaactgg
    22441 tcgcttcccg tcccgccgtt gatcccgtcg cggcccgtcc cgccgatgat ggtgtcgttg
    22501 ccattgccgc cgagcagctt gtccgcgtcg gagccgccgg cgagccggtc gtcgcccccg
    22561 ccaccgacca ggaggtcgag gccgccctcg ccgtagagga ggtccctgcc ggcgccgccg
    22621 tcgagaagat cgtcgccgcc gctcgccgcg acccaatcgt cgcctgcttc gccataggcg
    22681 cgatcgtcat tctgcccggt ctcgaaacgg tcgttgccgt cgccgccgta gaacaggtcc
    22741 gcgccggcgc cggtccggat cgaatcgtcg cccgcgccgg tcatgatccg gaagctctcg
    22801 acacccacga agtcgagcgt ggcgccggcg aggtcggcga tgtggccggt ataaccggtg
    22861 gccgcattgc cgctttcgac gccgcccgac accgaggtga tggcggccga ataatcgagc
    22921 accagccggt cgtcgccggc cccggcgtcg accgagtcgg cgccgccgac gacggtgatc
    22981 cgatccgcgc cgccgcccga cacaaccgtg tcggcgtcgg ctccggtgac gatctcgtcg
    23041 gagccgccgc cggtggtgat ggtgttctcg ccgtcgccgc cgcgcacgaa attgccgccg
    23101 tcggacgcgg tgatcgtgtc cgccccgtcg ccgccgacga tatagttcgc gccctggccg
    23161 gcggtgatcg tgccggcgcc gccatgggtg cggatgatgt cgtcgccgtc gccggtcgtg
    23221 atggtgtcgg cgccgctgcc ggtgacgatg gtgaagtcct cgaagccgcc attgacggtg
    23281 accatgcggg cgccgccgcc ggcctcggtg aagttggaag cggaatcgcc ggtcacggcg
    23341 ccggtggcga gccgataatc gaccaccagc cggtcgtggc cgccgccgcc ggagaccgcg
    23401 tccgagcccg cgctgacgcg gacgacattg tcgccgtcat tgccggtgat ggcattgccg
    23461 tcggccgcgt cgaacaggtc gatcggcgca ttgccggcga gcgtcacgac ggcgatgccg
    23521 gtggcgagcc gcagcacgat gccggtcgag ttcgcctcgc cggccacgat catgccgcta
    23581 tgggtgacgg tcgcggtttc gttcgaatag ccgccctcga gctggatggt gtcgttggga
    23641 ccggccgcgg ccatggcgtc ggcgatcgaa ggaaaggtcg aattgggtcc gacggtgaga
    23701 atgggcatgg gaatactcct ggcggggcgg caaggagctg catgcatccg ggtcgcgtac
    23761 gcaacttact cgcgcggatc gccgcgccga cttgtgcaga acatccggct gcttaatttg
    23821 ggagaatatt cgatatgcct aaccatatca taatattctg cagcaggcgg tatgggtaga
    23881 tcattaggct aatgtattga atcgtcaagt actattacgt tgcttgcttc ttttgtagat
    23941 aaggtggtgc taatatggcg cggtcgagcg gctatttagt tccgcagcgt cggaaatact
    24001 gcggcggcgc aagggtggaa gtgttccgcc gtcaagtggc gccggacctc atttcagccg
    24061 cgcgccatac cagtcgaggt agcgctgcag ccggtcgcgg acgaagctcg ggcgcttcaa
    24121 accgtgatat tcaccgggat agatgacgag gcccgtatcg acgccgcggc tcttcagcgc
    24181 ctggtacatt tgctccgagc cgagcagcgg cacgttgaaa tcctgctgcc cgaccaggaa
    24241 cagggtcggc gtcgtaatcc ggccggcctg gaagaagggg taggagaccc gcatccaggt
    24301 ctcgggcttc tcccaaggct tgccgagctc gagctcgtaa tcgcgtatat attggtcggt
    24361 gccgtagccc gacaggatgt tggcgatgcc ggcgccgctg gtcgcggcct tgaagcgggt
    24421 gtcgctggcg atgacgtaat tggtcagcat gccgccatag ctccagccgc cgacgccgag
    24481 ccggttcgga tcggcgatgc cgcgcgccac cgcatcgtcg accgcggcga gcacgtcctt
    24541 gacgtcgacc ccgccccagg cggcgtagat cgcccgggaa taatcctcgc cgcggccggt
    24601 gctgccgcgc ggattggcgg cgaccacggc atagccgttg gcggcgaata gctgccattc
    24661 gaagctgaag ccgtgattat attggctggc cggcccgccg tggatgcgga gcagggtcgg
    24721 atgcggcgcc ccggcggggg cgcccagcgg ccgggtgagg aagccgtgca cttcggtgcc
    24781 gtcggtgctg gtgaagcggg tgagttccac cggcgcaagc tggagccccg ccagccaggc
    24841 ctcgttctgc cgcgacagcg gccgcagctt gccgccttcg agcgcataga cttcgggcgg
    24901 agtggcggga tcgctgacca agacggcggt cttgccggcg gcgttggcgt ggatcgcgtc
    24961 gaagatgcgg cggccgccgg cgacttcggc cagcttgccg ccggccgcgg gcacccgcgc
    25021 cacccattcg ccgccatcgt cctcgacggt gaagcgcagc gaccggccgt cgggggacca
    25081 ggtcggatgg aggac
//
LOCUS       DASVRK010000307         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_164646, whole genome shotgun sequence.
ACCESSION   DASVRK010000307 DASVRK010000000
VERSION     DASVRK010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164646"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VF589_12895"
     CDS             <1..1273
                     /locus_tag="VF589_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258521.1"
                     /translation="ANIETLALTGGGDVDGTGNELANLILGNDGANRLDGRGGADTMR
                     GGGGDDVYAVDEAGDRAVELAGGGRDRVETALDFTLGAGLEDLTLGGAAVAGTGNALA
                     NEIIGNGAHNRLDGRAGADRMAGGGGDDLYVVDDAGDAVVESDGEGSDRVEASVSYAL
                     GANVEALALVGPDAIEGTGNALANTITGNAAANRLDGAAGADRMIGGAGDDTYLVEAF
                     EDEIIEGASGGIDTVLSSGSYRLAGWVERLVLVGEATDGIGNALDNEIIGNDGINVLD
                     GGDGDDRLVGGGGLDIMKGGDGGDRLDGGGGRDLLTGGAGADHFLFGAPIDGVVNIDD
                     LTDFVAGEDSLHLDRAVFEGIAADGALGEEAFHVGAEAADAGDRLIYNQTSGEIFYDA
                     DGTGAAAAILFARVEAGTVLTHLDFAAYSQG"
     gene            complement(1287..1787)
                     /locus_tag="VF589_12900"
     CDS             complement(1287..1787)
                     /locus_tag="VF589_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8258522.1"
                     /translation="MIRQYGVLAYDMDGAEPRFLLITSRRTRRWIIPRGNPIRGLSAS
                     ETAAQEAHEEAGLIGVIGKQEIGSYRYVKWRRDGSNVMAKVHVFPFRATLQSGRWRER
                     DQRDWRWFTRAEAAAAVEEPELKQLILAFTPPPESAGAFHGTPPPRVRLSLLRLLRKL
                     VPGKGG"
     gene            1915..2106
                     /locus_tag="VF589_12905"
     CDS             1915..2106
                     /locus_tag="VF589_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258523.1"
                     /translation="MRTGYLLTLWVLSALTLGLQATTGAEQGMLPVSILLMIPLVVMA
                     TEDARNRRHSYRTASREPR"
     gene            2148..2945
                     /locus_tag="VF589_12910"
     CDS             2148..2945
                     /locus_tag="VF589_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8258524.1"
                     /translation="MADRTNGAALAAMALFAHELADAARRQTLPLWASGCAADDKGGP
                     GGFDPVTAADRGAEQAMRALIEAHFPEHGIMGEEFADRPASGRYSWSLDPVDGTRSFI
                     CRLPTWVTLIALLDEGAPVLGLIDAPCLDERYVGFGPGSFRAGGRESQLATSGCTRLE
                     EARLSTTDPYMFEGARQEGWERVRGRVRTTRYGHDGYGYARVAAGSLDLVIETGLKPH
                     DYNALIPVVRSAGGAIGDWEGGEDFSASHIVAAATPELYEAAVALLR"
BASE COUNT          489 a         1117 c         1136 g          464 t
ORIGIN      
        1 ggccaatatc gagacgctcg cccttaccgg cggcggtgac gtggacggca ccggcaacga
       61 actcgccaac ctcatccttg gcaatgacgg cgccaaccgc ctcgacggcc gcggcggcgc
      121 tgacacgatg cgcggcggcg gcggcgacga cgtctatgcc gtggacgaag ccggcgaccg
      181 cgccgtcgag ctcgccggcg gcggccgcga ccgcgtcgag accgcgctgg atttcacgct
      241 tggcgcgggg ctcgaggatc tgaccctcgg cggcgcggcg gtggccggca ctggaaacgc
      301 gctggccaac gaaatcatcg gcaacggcgc gcacaaccgc ctcgacgggc gggccggagc
      361 cgaccggatg gccggcggcg gcggcgacga tctctacgtc gttgacgatg ccggcgatgc
      421 ggtcgtcgaa agcgacgggg agggcagcga ccgggtcgaa gccagcgtca gctacgccct
      481 cggcgcgaat gtcgaagcgc tcgccctcgt cgggccggac gccatcgaag gcaccggcaa
      541 cgcactcgcc aacacgatca ccggcaacgc cgccgccaac cggctcgacg gcgcggccgg
      601 ggccgaccgg atgatcggcg gcgccggcga cgacacttac ctcgtcgaag cctttgagga
      661 tgagataatc gagggcgcct cgggcggaat cgacacggtg ctgagctcgg gcagctatcg
      721 cctcgccggc tgggtcgagc ggctggtcct ggtcggcgag gctaccgacg gcatcggcaa
      781 cgcgctcgat aacgagatta tcggcaatga cggcatcaac gtgctcgacg gcggcgacgg
      841 cgacgaccgt ctggtgggcg gcggcgggct cgacatcatg aagggcggcg acggcggcga
      901 ccgcctcgac ggcggcggcg gccgcgacct gctgaccggc ggcgccggcg ccgatcattt
      961 cctgttcggc gcgccgatcg acggcgtggt caacatcgac gacctcaccg atttcgtggc
     1021 cggcgaggac agccttcatc tcgaccgcgc ggtgttcgag ggcatcgcgg ccgacggcgc
     1081 gctcggcgag gaggccttcc acgtcggagc ggaggcggcc gatgcgggcg accgactgat
     1141 ctacaaccag acgagcggcg agattttcta cgatgccgac ggcaccggcg cggcggcggc
     1201 gatcctgttc gcgcgggtcg aggccgggac ggtgctcacc cacctcgatt tcgccgccta
     1261 tagccagggt tagacgccgc tccgggtcag ccgcctttgc cgggcactaa cttcctgagc
     1321 aggcggagca gcgacaaccg cacgcgcggc ggcggcgtgc cgtggaaggc gccggcactc
     1381 tccggagggg gcgtgaaggc aaggatcagt tgcttgagct ccggctcctc gaccgccgcc
     1441 gcggcttccg cgcgggtgaa ccagcgccag tcgcgctgat cgcgctcgcg ccagcgcccg
     1501 ctctggagcg tggcgcggaa tgggaagaca tggaccttgg ccatgacgtt ggagccgtcg
     1561 cgccgccatt tcacgtagcg gtagctgccg atctcctgct ttccgatcac gccgatcagc
     1621 ccggcctcct catgcgcctc ctgcgcggcg gtttcggatg ccgaaagccc gcggatcggg
     1681 ttgccgcgcg ggatgatcca ccgcctggtc cggcgcgagg tgatcaggag aaagcgcggc
     1741 tccgctccgt ccatgtcata ggcgagaact ccatattgcc ggatcacctg acatgctccg
     1801 cgggcgggaa aagccaaatg tagcagcggc atcggcccgg cggcaatgcg gcggggcagg
     1861 gcggcgcccg gccagatacg attcgttaat gcctcgcggc gcactatcgc cctcatgcgg
     1921 acaggatatc ttcttacctt gtgggtgctg agcgccctca cgctcggcct tcaggccacc
     1981 accggagccg agcagggcat gttgccggtc tcgatcctgc tgatgatccc gctggtcgtc
     2041 atggccaccg aggacgcgcg gaaccgccgc cattcctacc gcaccgcgag ccgcgagccc
     2101 cgataagccg cttgacgcgt gggaccccgg tccttcacgc gggcgccatg gctgaccgaa
     2161 ccaacggcgc ggcgctcgcc gccatggccc ttttcgcgca cgagcttgcc gatgccgcgc
     2221 gccggcagac gctgccgctt tgggcctccg gctgcgcggc cgacgataaa ggcggcccgg
     2281 gcgggttcga tcccgtcacc gccgccgacc ggggcgccga acaggcgatg cgggcgctga
     2341 tcgaggcgca cttccccgag catggcatca tgggcgagga gtttgccgac cgccccgcct
     2401 ccggccgcta tagctggagc ctcgatccgg tcgacggcac gcgctccttc atctgccggc
     2461 tgccgacctg ggtgaccttg atcgccttgc tcgacgaggg tgccccggtg ctcggcctga
     2521 tcgacgcgcc gtgcctcgac gaacgctatg tcgggttcgg ccccggcagc ttccgggccg
     2581 gcggccgcga aagccagctc gccacgagcg gctgcacgcg gctcgaagag gcgcggcttt
     2641 cgaccaccga tccctatatg ttcgagggcg cgcgccaaga aggctgggag cgggtgcgcg
     2701 gccgcgtccg caccacccgc tacggccatg acggctatgg ctatgcccgc gtcgccgccg
     2761 gctcgctcga tctggtcatc gagaccggcc tcaagccgca cgattacaac gcgctgatcc
     2821 cggtggtgcg ttcggccggc ggagcgatcg gcgattggga gggcggggag gatttctccg
     2881 ccagccacat cgtcgccgcc gcgacgccgg agctgtatga ggcggcggtg gcgttgttgc
     2941 gctagcacgt caggccggac gccctcgtca tgccggactt gatccggcat cccgcttctt
     3001 cttccttgat actggtgaga agaagaaagc aggccaatcc cagcctgcgc catgtgcccc
     3061 tgcgaaggca ggggcccaga cgcgggctga cgctccagcg gcagcccggt cctcggagcg
     3121 gtcatggagc cggcgccgta gcccgcacgg tcccggactc gcgcgacgcc taggagagcg
     3181 tggtggcggc ccctgccttc gcaggg
//
LOCUS       DASVRK010000308         5927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_164897, whole genome shotgun sequence.
ACCESSION   DASVRK010000308 DASVRK010000000
VERSION     DASVRK010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164897"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="VF589_12915"
     CDS             complement(<1..1566)
                     /locus_tag="VF589_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEX8258525.1"
                     /translation="MADRAFEELPAGAEEAPPPRRRGRWWSRIAKALVGLFLLALIAL
                     AGLVAFLDTDAGHRWIADRIAAQTPESGLDIRIGRIDGSIWGDTLLRDVRLYDPDGLF
                     AESPRIELEWQPLGWIANRLLIDELRSELVIVHRLPRLRPAPEPRPILPDFDIRIGRL
                     EVAQLRFEKAVTGQRRVASLAGGVDIRSGRALVDVRARVSGGGDDLALVLDAEPDRDH
                     FDIDVRLNAPANSVTGAIVGTKRPIQLEVKGDGRWSAWAGTARLNLSGRRTADLALRA
                     DSGRYSLSGSLTPSQFLKGKQQRLTAPRVLLNARATLADRRLDGSLSLRSSALKVEAK
                     GAIDLARSAYDEVSIAADLLQPAALFPNMTGQKVRLTALLDGAFRRADFAYRITSPRI
                     AFDDTGFEEVRAEGKGRLSREPVTVPVRFTARRVTGVGDVAGGILANLRIEGLLKVTR
                     RRLTGEGLALTSDKLNGKLSLFVDLVNGQYDVILSGGLARYLIPGLGIVDVLTELKVV
                     EGPGGRGTMVTGRG"
     gene            complement(1566..3587)
                     /locus_tag="VF589_12920"
     CDS             complement(1566..3587)
                     /locus_tag="VF589_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8258526.1"
                     /translation="MAAATAVAALFFGGIEARAQQADAFEELSAPLDPAAPLDPYAPL
                     DQAPLDQAPLDPSAPLDPMPDLGVDWPDMDAPAGEEIADVPDTAIADAATERSYNVRI
                     AGLESIEAAGLMTQFAELSTLEANREDPANAAQIDRRAREDADLLAELMRAHGFYDAL
                     VTTRVEPGGAAGAMLVTLEVQPGAQYRFAEVRLPGLEAAGAEAEALRRVFGVEPGEAV
                     NAAQVTAGEAALRAELGRRGFAFAEVGALDIAVDHAARTATLALPVDPRGAREFGRIL
                     VEGKPLFDAEHIEGIARFEPGDKFEAPLLEDLRRALVATGLVSTVAIRPVDNPASRRV
                     DIAVALEPAPMRTIAGAAGYGTGEGFRVEASWQHRNLIRPEGAVTLRSVLGTREQLIA
                     ATLRRSNFRRRDQVLTAQVSASHIERDAFDARTFLVGAGIERQTNIIWQKKWTWSLGA
                     DLIASDERDVDVETGLTRRRTFFIGALPGTLAYDGSNDLLDPTSGYRLSGRFSPEASL
                     QDGSFFYAKAQVDGSFYQPVSERVVIAGRARLGTIVGADRDRIAPSRRFYAGGGGSVR
                     GYGFQELGEQDPVFEDPIGGRSLAEFAIEARVRVNALGDKLGIVPFIDAGNVYASSTP
                     QLDDLRFGAGIGVRYHTNFGPIRVDIGTPINPRPGDARAAVYVSLGQAF"
     gene            complement(3707..3901)
                     /locus_tag="VF589_12925"
     CDS             complement(3707..3901)
                     /locus_tag="VF589_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258527.1"
                     /translation="MADRAAVTEATDLIDRFGELARLEAANRADRSRSLGNVIHFCHW
                     RQVERAIDMLCAEDVTGTVH"
     gene            4062..4229
                     /locus_tag="VF589_12930"
     CDS             4062..4229
                     /locus_tag="VF589_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258528.1"
                     /translation="MDEKGTPRAGGFILAASIIAGVVGGTVAGQPSAGFLVGVAAGAI
                     LALIIWWKDRR"
     gene            complement(4237..4890)
                     /locus_tag="VF589_12935"
     CDS             complement(4237..4890)
                     /locus_tag="VF589_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX8258529.1"
                     /translation="MDARATLERLIEERGEDYASLSRLLGRNPAYVQQYIKRGSPRRL
                     SEEDRRLLARYFGVAEALLGGPEGGSAGARATGPNYVVVPQLDVGASAGSGALPGEER
                     ALSHIAFDARWLRSLGTGDPGQLSVIRVEGDSMTPALADGDDILVDRGDAAQRLRDGI
                     YVLRFEEALIVKRVALNPAGRTVSIRSDNPAYPSWPDCDLAAVDIVGRVVWTGRRLA"
     gene            4984..5184
                     /locus_tag="VF589_12940"
     CDS             4984..5184
                     /locus_tag="VF589_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258530.1"
                     /translation="MYLLKRIESYLRKSGTPATKFGREVLGDPRFVFDLRKGREPREA
                     TEARVASYIDAHEVEARPPCNR"
     gene            5172..5510
                     /locus_tag="VF589_12945"
     CDS             5172..5510
                     /locus_tag="VF589_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258531.1"
                     /translation="MQPLINDATTAILRAIRLNFLNFQNLRFEEIRSRSWASATFSGA
                     RHELTLLLEGDGAAEAANAFVHGLEAAEFTLRGHIMADIGVVADERGAALGGAPQVRL
                     RIEALTVEEG"
     gene            complement(5513..>5927)
                     /locus_tag="VF589_12950"
     CDS             complement(5513..>5927)
                     /locus_tag="VF589_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258532.1"
                     /translation="QAEEPSAEDNFDNSWDEAPATEAYAEPEPEPEPEPEPEPEPEPE
                     PEPEFTPEPEPEPEFTPAPAAEYSPPPAAPAPPAAGAEQPSIAHLMQRLERGLDRRRP
                     GDAFPAEAVLELRTPAQGRLRNALDDLQKRARRGG"
BASE COUNT          873 a         2120 c         2085 g          849 t
ORIGIN      
        1 gccgcggccg gtcaccatcg tgcccctgcc gcccggcccc tccaccacct tgagctcggt
       61 gagcacgtcg acgatgccga gcccggggat gagatagcgg gcgaggccgc cggagaggat
      121 cacgtcatat tggccgttga ccaggtcgac gaacaggctg agcttgccgt tcaacttgtc
      181 cgaggtcagc gccagcccct cgccggtgag ccggcgccgg gtcaccttga gcagcccctc
      241 gatccgcaga ttggcgagga tgccgccggc gacgtcgccg acgccggtga cgcggcgggc
      301 ggtgaagcgg accgggacgg tgaccggctc gcgcgacagc cgccccttgc cttccgcgcg
      361 cacctcctcg aacccggtgt cgtcgaacgc gatccgcggc gaggtgatcc ggtaggcgaa
      421 gtcggcgcgg cgaaacgcgc cgtcgagcaa ggccgtgagc cggaccttct gcccggtcat
      481 gttcgggaac aaggcggcgg gctggagcag gtcggcggct atgctgacct cgtcataggc
      541 gcttcgggcg aggtcgatcg cccccttcgc ctccaccttc agcgccgacg agcgcagcga
      601 caggctgccg tccagccggc ggtcggcgag cgtggcgcgg gcgttgagca gcacccgcgg
      661 cgcggtcagc cgctgctgct tgcccttcag gaattgcgac ggggtcagcg agcccgacag
      721 gctgtagcgg ccgctgtcgg cgcgcagggc aaggtcggcg gtgcggcggc cggagaggtt
      781 cagccgcgcc gtccccgccc aggcgctcca gcggccgtcg cccttcactt cgagctggat
      841 cgggcgcttg gtgccgacga tcgcgcccgt gacgctgttg gcgggcgcgt tcaaccgaac
      901 gtcgatgtcg aaatggtcgc ggtccggctc ggcgtcgagg acgagggcaa ggtcgtcgcc
      961 gccgccgctc accctggccc gtacgtcgac gagcgcgcgg ccggagcgaa tatcgacccc
     1021 gcccgccagg ctagccaccc gccgctgccc ggtgaccgcc ttttcaaagc ggagctgggc
     1081 gacttcgagc cgcccgatcc tgatatcgaa atcgggcagg atcggccgcg gctcgggcgc
     1141 cggccgcagc cgcggcagcc ggtggacgat gacgagctcg gaccgaagct cgtcgatcag
     1201 cagccggttg gcgatccagc ccagcggctg ccattcgagt tcgatgcgcg gcgattcggc
     1261 gaacagcccg tcggggtcgt aaagccgcac gtcgcgcagc aaggtgtcgc cccagatcga
     1321 cccgtcgatc cggccgatgc ggatgtcgag ccccgattcc ggcgtctgcg ccgcgatccg
     1381 gtcggcgatc cagcgatgcc ccgcgtcggt gtcgagaaag gcgaccagcc cggcgagcgc
     1441 gatcagggcg agcaggaaca gccccaccag cgccttggcg atgcgcgacc accagcggcc
     1501 gcgccggcgc ggcggcggag cctcctcggc gcccgcgggt agctcctcga aggcgcggtc
     1561 ggccatcaga aggcctggcc gagcgacacg tagacggcgg cgcgggcgtc cccgggccgc
     1621 gggttgatcg gggtgccgat atcgacccgg atcggcccga aattggtatg gtagcggacg
     1681 ccgatgccgg cgccgaagcg caggtcgtcg agctgcggcg tcgaggaagc atagacgttg
     1741 ccggcgtcga tgaacggcac gatgccgagc ttgtcgccca acgcgttgac gcgcacccgc
     1801 gcctcgatcg cgaattcggc gaggctgcgg ccgccgatcg ggtcctcgaa caccggatcc
     1861 tgctcgccca gctcctggaa gccgtagccg cgcaccgacc cgccgccgcc ggcatagaag
     1921 cgccgcgacg gagcgatccg gtcgcggtcc gcgccgacga tggtgccgag ccgcgcccgc
     1981 ccggcgatca ccacccgctc cgacaccggc tggtagaagc tgccgtcgac ctgcgccttg
     2041 gcatagaaga agctgccgtc ctggagcgag gcctcgggcg agaagcggcc ggacaggcgg
     2101 tagcccgagg tcggatcgag caggtcgttc gacccgtcat aggcgagcgt gcccggcagc
     2161 gcgccgatga agaaggtccg gcgccgcgtc agcccggtct cgacatcgac gtcgcgctca
     2221 tccgaggcga tcaggtcggc gccgagcgac caggtccatt tcttctgcca gatgatgttg
     2281 gtctgccgct cgatcccggc gccgaccagg aaggtgcgcg catcgaaggc gtcgcgctcg
     2341 atgtggctgg ccgagacctg ggcggtgagg acctggtcgc gccgccggaa attgctgcgc
     2401 cgcaaggtgg cggcgatgag ctgctcgcgg gtgccgagca cgctccggag cgtcaccgcc
     2461 ccttcgggcc gtatcaggtt gcgatgctgc cagctggcct cgacccggaa cccctcgccg
     2521 gtgccgtagc cggccgcgcc ggcgatggtc cgcatcggcg ccggctccag cgccaccgcg
     2581 atgtcgaccc ggcggctggc gggattgtcg accggccgga tcgcgaccgt cgacaccagc
     2641 cccgtcgcaa ccagtgcgcg gcgcagatcc tcgagcagcg gcgcttcgaa cttgtcgccg
     2701 ggctcgaacc gggcgatgcc ctcgatatgc tcggcgtcga acagcggctt gccctcgacg
     2761 aggatgcggc cgaactcgcg cgcgccgcgc ggatcgacgg gaagtgccaa agtcgcggtg
     2821 cgggcggcat ggtcgacggc gatatccaga gcgccaacct cggcgaaggc gaagccgcgc
     2881 cgcccgagct cggccctaag cgccgcctcg ccggccgtca cttgggcggc attgaccgct
     2941 tcgcccggct cgaccccgaa cacccggcgg agcgcctccg cctcggcgcc ggcggcttcg
     3001 aggcccggca gccgcacctc ggcgaagcga tattgcgcgc ccggctgcac ctccagcgtc
     3061 accagcatcg cgccggccgc accgcctggc tcgactcggg tggtcaccag cgcatcgtaa
     3121 aagccgtgcg cgcgcatcag ctcggcgagc aggtcggcat cctcgcgggc gcgccggtcg
     3181 atctgggcgg cgttggcggg gtcctcgcgg ttcgcctcca gcgtcgacag ctcggcgaac
     3241 tgggtcatca gcccggccgc ctcgatgctc tccaggccgg cgatcctgac attgtagctg
     3301 cgctcggtgg cggcgtcggc gatcgcggta tcggggacgt cggcaatctc ctcccccgcc
     3361 ggcgcatcca tgtccggcca gtcgacgccg aggtccggca tcgggtcgag cggagcggac
     3421 gggtcgagcg gtgcctggtc gagcggggcc tgatcgagcg gggcgtaagg gtcgagcgga
     3481 gcggcggggt cgagcggagc gctcagttcc tcgaaagcgt ccgcttgctg cgcccgcgcc
     3541 tcgatcccgc caaagaaaag ggcggcgaca gccgtggcgg ccgccacacg ccgccccgcc
     3601 gcgacttgta acatgggatg aaactagcgg gcgcgcctgt accgcgccag aacagactta
     3661 accttgtcgc cgcttccgct cgcccgcgcc gaccgggcgg cgacggtcag tggacggtgc
     3721 cggtcacgtc ctcggcgcag agcatgtcga tcgcccgctc cacctgccgc cagtggcaga
     3781 aatggatgac gttgccgagc gagcggctgc gatccgcgcg attggccgct tcgagccgcg
     3841 ccagctcgcc gaaccggtcg atcagatcgg tcgcctcggt caccgccgcg cgatccgcca
     3901 caggaacatg ttgccgcaaa ttccccgccc tctttatttg cttacccctg ccccctctag
     3961 ggccgccgcg tttcgaaatg ccgcaccgcc atcctcaacg ccccgttcac cgcgttcggc
     4021 cgggctggaa ctggcgcggc gaggctgcca tgggtccggc catggacgag aaaggcactc
     4081 cccgcgccgg cggcttcatc ctggcggcca gcatcatcgc cggcgtggtc ggcggcaccg
     4141 tcgccgggca gccgagcgcc ggcttcctcg tcggcgtcgc cgcgggcgcc atattggcgc
     4201 tgatcatctg gtggaaagac cggcgctaac gccggttcag gccagccgcc ggccggtcca
     4261 caccacccgt cccacgatgt cgaccgctgc gaggtcgcag tccggccaac ttgggtaagc
     4321 gggattgtcg ctccggatcg acaccgtccg gccggccggg ttcagcgcca cgcgcttgac
     4381 gatcagcgcc tcttcgaacc gcagcacata gatgccgtcg cgcaagcgtt gcgcggcatc
     4441 gccgcgatcg accaatatgt cgtcgccatc ggccaaagcc ggcgtcatcg agtcgccctc
     4501 gacgcggatc accgacagct ggcccggatc gcccgtcccg agcgagcgca gccagcgcgc
     4561 gtcgaacgcg atatgcgaca aggcccgctc ctcgcccggc agcgcaccgg agccggccga
     4621 ggcgccgacg tcgagctgcg gcaccaccac ataattgggt ccggtcgccc gcgcgccggc
     4681 tgatccgccc tccggcccgc cgagcagtgc ttccgccact ccgaaatagc gggcgagcag
     4741 ccggcggtcc tcctcgctca gccgccgcgg gctgccgcgc ttgatatatt gctgcacata
     4801 cgccggattg cggccgagca gacgcgacag gctggcataa tcctcgcccc gctcctcgat
     4861 cagccgctcc agtgtcgcac gcgcatccat gaccgcacca tagcggtagg atttttccta
     4921 gacaagtagg aaacttccta tgacggtgaa catatcagga acagtgattc ggagagggac
     4981 tgaatgtatc tgctcaagcg tatcgagagc tatctccgga aaagcgggac tcccgccacc
     5041 aagttcggtc gcgaggtcct gggcgacccg cgcttcgtct tcgaccttcg caaggggcgc
     5101 gagccgcgcg aagccaccga ggcccgggtc gcctcctata tcgacgccca cgaagtggag
     5161 gcgcgcccgc catgcaaccg ctgatcaacg atgcgacgac cgcgattctg cgggccattc
     5221 gcctaaactt cctaaacttc cagaacctgc gcttcgagga aatcaggagc cgcagctggg
     5281 ccagcgccac cttcagcggc gctcgtcacg agcttacctt gctgctcgag ggcgacggcg
     5341 ccgccgaagc cgccaatgcc ttcgtgcacg ggctggaagc cgccgagttc acgcttcgcg
     5401 gccacatcat ggccgatatc ggcgtggtcg ccgacgagcg cggcgcggcg ctcggcggcg
     5461 ccccgcaggt gcggctcagg atcgaggcgc tgacggtcga ggagggctga gctcagccgc
     5521 cgcggcgggc gcgcttctgg aggtcgtcga gggcgttgcg cagccggccc tgggcggggg
     5581 tgcgcagctc caacaccgcc tcggcgggga aggcgtcgcc cggccggcgg cggtcgagcc
     5641 cgcgctccag gcgctgcatc agatgcgcga tgctcggctg ctcggctccg gccgcgggcg
     5701 gcgcgggcgc cgcggggggt gggctgtatt ccgcagcagg cgcgggcgtg aattcgggct
     5761 cgggctcggg ctcgggcgtg aactccggct cgggctcggg ctcgggctcg ggctcgggct
     5821 cgggctcggg ctcgggctcg ggctcgggct cggcataggc ttcggtcgcg ggcgcttcat
     5881 cccagctatt gtcgaaatta tcctcagcgc tgggctcctc cgcctgc
//
LOCUS       DASVRK010000309         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_556449, whole genome shotgun sequence.
ACCESSION   DASVRK010000309 DASVRK010000000
VERSION     DASVRK010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556449"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VF589_12955"
     CDS             <1..615
                     /locus_tag="VF589_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258533.1"
                     /translation="VAALEQRIGHIDMQSRAAVGNADRAEGLLVAFAARRALDRGVAL
                     GYIEGLLRDRFGETQRPAVATIITASRQPVTLEELQLGLQEVGPDLTGAAPNQDLWSA
                     FKTELSGLITVRKSGTPSTLPAERLLRATRRLEAGQVDVALAEVLRMPGRENAGEWVA
                     AAKRYVDARRALDTIETAALLDPRNPPQTPAQARRAMASPRAAS"
     gene            complement(612..1343)
                     /locus_tag="VF589_12960"
     CDS             complement(612..1343)
                     /locus_tag="VF589_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8258534.1"
                     /translation="MNDHPTDRLIVAAIGDLHVTESADQRFHELFAEISDAADVLALC
                     GDLTNFGKTHEAELLAEDLRGCTIPTVAVLGNHDYECGQPEEVARILHQAGVTMLDEQ
                     AVVIEGVGFAGVKGFVGGFGRGELGAFGEHAIKTFVDESRNEARKLENQLRSLRTDRT
                     VAVLHYAPVADTVEGEPLEIFPFLGSSRLAHAVDRFDNVKAVVHGHAHRGSYEGKTAG
                     GVPVYNCARFVVEQAMGRPYALLEI"
     gene            complement(1324..2115)
                     /locus_tag="VF589_12965"
     CDS             complement(1324..2115)
                     /locus_tag="VF589_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258535.1"
                     /translation="MSDGLQIIDAAAFEPPPEAVDFYIESLRLLNESGVPFLLSGTYA
                     LGCFTGIVRPTKDLDVFCKPSDAPKILAFFKSRGYAIEIEDERWIGKVWRGDNFFDVI
                     YNISSASIPITDEWFKEVCEAEVYGTTVRITPPTQFILSKLFLQTRYRYDGADVAHTI
                     LRKADDIDWGWLLSSLELYWEVLLINVLNFRFIYPSERDLVPRWLFDELMERLAAQSE
                     MPPPGIRICRGRLLSPTDFVVDVSEWGFADVVGKGIDEKHERPSH"
     gene            complement(2220..>3307)
                     /locus_tag="VF589_12970"
     CDS             complement(2220..>3307)
                     /locus_tag="VF589_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258536.1"
                     /translation="SAKGGTGTDTIRLSFSTSGVGENFVFSTGLNNMNNGADFDGFER
                     LDFAGGTGIDRVTGGALDDIVYGGGNNDVLHGGGANDRVVGEDGDDEVYGDAGDDSLW
                     DNGTGNDTLDGGIGNDTIYSGDGADKLIGGSGDDTFHMEDDYEGDFYNGTGNWMGDVV
                     NGGTNIDKVSYYPSFSSIYLDVATQTANDGAAKGDRFSGVEIFEGSFYDDVMLGDVAA
                     NTFLGGGGDDHMDGRGGNDRLVGGSGPDTMTGGAGVDTFVLDNGSNYFLGTDWSSDVI
                     TDFVSGTDKLEISLYEFGLAEGQDVGLEVGVDPAAQGTGAQFLFDTASGRLWFDSDGT
                     SENESPILIATLDNVTKVTVADFVIVA"
BASE COUNT          554 a         1058 c         1119 g          576 t
ORIGIN      
        1 gtggcggcgc tcgagcagcg catcggccat atcgacatgc aatcgcgcgc cgcggtcggc
       61 aatgccgacc gggccgaagg gctgctggtg gcgttcgccg cccgccgtgc gctcgatcgc
      121 ggcgtcgcgc tcggctatat cgaagggttg cttcgcgacc gcttcggcga aacccagcgt
      181 ccggcggtgg ccacgatcat caccgcctcg cgccagccgg tcacgctgga ggagcttcag
      241 ctcgggctcc aggaggtcgg cccggacctc accggcgccg cgcccaatca ggatctgtgg
      301 agcgcgttca agaccgagct ttcgggcctg atcacggtcc gcaagagcgg caccccttcc
      361 accttgccgg cggaaaggct gctgcgggcg acgcggcggc tggaggcggg gcaggtcgac
      421 gtggcgctcg ccgaagtgct gcgcatgcca ggccgcgaga atgccggcga atgggtggcc
      481 gccgccaagc gttatgtcga tgcgcggcgg gcgctcgaca cgatcgagac cgccgctttg
      541 ctcgatccgc gcaacccgcc gcagacgccc gcgcaggcgc gccgcgccat ggcctcgccc
      601 cgcgccgcca gctagatttc gagcaaggcg taggggcggc ccatcgcctg ctcgaccacg
      661 aagcgcgcgc aattgtagac gggaacgccg ccggcggtct tgccttcgta gctgccgcga
      721 tgggcatggc cgtggaccac cgccttcaca ttgtcgaatc ggtcgacggc atgggcgagg
      781 cgcgaggagc ccaggaaggg gaagatttcg agcggctcgc cttcaaccgt gtcggcgacg
      841 ggcgcataat ggagcaccgc gaccgtgcgg tcggtgcgca gggagcgcag ctggttttcg
      901 agcttgcgcg cttcgttgcg cgattcgtcg acaaaggtct tgatcgcatg ctcgccgaag
      961 gcgccgagct cgccccggcc gaagccgccg acgaagccct tgaccccggc gaagccgacg
     1021 ccctcgatca ccaccgcctg ctcgtcgagc atggtgacgc cggcctggtg gaggatgcgc
     1081 gcgacctctt cgggctggcc gcattcgtaa tcgtggttcc ccagcacggc cacggtcggg
     1141 atggtgcagc cgcgcaggtc ctcggcgagc agctcggcct cgtgcgtctt gccgaaattg
     1201 gtgaggtcgc cgcacaaggc gaggacgtcg gcggcgtcgg agatttccgc gaatagctca
     1261 tggaagcgct gatccgcgga ttcggtgacg tggaggtcgc cgatcgcggc gacgatcagc
     1321 cggtcagtgg gatggtcgtt catgcttctc gtcgatccct tttccgacga cgtcggcgaa
     1381 gccccattcg ctgacgtcga ccacgaagtc ggtggggctg agcaggcggc cgcgacagat
     1441 cctgatgccc ggcggcggca tttccgactg cgcggccagg cgctccatca attcgtcaaa
     1501 cagccagcgc ggcacgaggt cgcgctcgga cgggtagatg aagcggaagt tgaggacgtt
     1561 gatcagcaaa acttcccaat agagttccag cgaggagagg agccagcccc agtcgatatc
     1621 gtccgccttc ctgaggatgg tgtgggcgac gtcggccccg tcatagcggt agcgggtctg
     1681 gaggaagagc ttggagagga tgaactgggt cggtggagtg atccggacgg tggtgccgta
     1741 gacctcggcc tcgcagacct ccttgaacca ttcgtcggtg atcgggatcg aggcggacga
     1801 aatgttgtag atgacgtcga agaaattgtc gccgcgccag accttgccga tccagcgctc
     1861 gtcctcgatc tcgatcgcgt agccgcggct cttgaagaag gcgaggatct tgggcgcgtc
     1921 gctcggcttg cagaagacgt cgaggtcctt ggtcggccgc acgatgccgg tgaagcagcc
     1981 gagcgcgtag gtgcccgaaa gcaggaaggg aacgccggat tcgttgagca gccgcaggct
     2041 ctctatgtag aaatccaccg cctccggggg cggttcgaac gccgcggcgt cgatgatctg
     2101 caatccgtcg gacatggcac ccttgctgct gccgggcgcg aatacggaaa gaagcgccgt
     2161 cccccgcccg gaagggaaga gaacggcgct tcattggaag cgttccgaag ccgccccgct
     2221 caggcgacga tcacgaaatc cgctacggtt accttggtga cattgtcgag ggtcgcgatc
     2281 aggatcggcg actcattctc ggaagtgccg tcggaatcga accacagccg gccgctcgcc
     2341 gtgtcgaaca ggaattgcgc gcccgttccc tgcgcggcgg gatcgacgcc gacctcgagg
     2401 ccgacatcct gcccttcggc aaggccgaat tcgtagagcg agatttcaag cttgtcggtg
     2461 ccgctcacga agtcggtgat cacgtcggac gaccagtcgg tcccgagaaa atagttactg
     2521 ccattatcga ggacgaaggt gtcgacgccc gcgccgccgg tcatggtgtc ggggccactg
     2581 cctccgacca gccggtcgtt gccgccgcgg ccgtccatgt gatcgtcgcc gccgccgccg
     2641 aggaaggtgt tggcggcgac atcgcccagc atgacgtcgt cgtagaagct cccttcgaaa
     2701 atctcgacgc cgctgaaacg gtcgcccttg gccgctccgt cgttcgcggt ctgggttgcg
     2761 acgtcgagat agatgctgct gaagctcgga taatagctga ccttgtcgat gttggtgccg
     2821 ccgttgacga cgtcacccat ccagttgccg gtcccgttgt agaagtcgcc ttcataatcg
     2881 tcttccatgt ggaaggtgtc gtcgccgctg ccgccgatca gcttgtccgc tccgtctccc
     2941 gaatagatcg tgtcgttgcc gatgccgccg tccaacgtgt cgttgccggt gccgttgtcc
     3001 cagagcgaat cgtcgccggc atcgccgtaa acctcgtcgt cgccatcctc gccgacgacg
     3061 cggtcgttcg cgccgccgcc atggagaacg tcgttattgc cgccgccata gacgatatcg
     3121 tccagcgccc cgccggtgac gcggtcgatg cccgtgccgc cggcaaagtc gagccgctcg
     3181 aagccgtcga agtccgcccc gttgttcatg ttgttgaggc cggtcgagaa gacgaaattc
     3241 tcgccgaccc ccgacgtgga gaaagacaga cggatggtgt cggtgccggt gccgcccttg
     3301 gcgctgt
//
LOCUS       DASVRK010000310         7101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_57132, whole genome shotgun sequence.
ACCESSION   DASVRK010000310 DASVRK010000000
VERSION     DASVRK010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57132"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VF589_12975"
     CDS             complement(<1..858)
                     /locus_tag="VF589_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX8258537.1"
                     /translation="MPTTGASAPSIADDPLQTSRSGGLRGRFAALAGRLEARLDAERD
                     QLPLWLPVGLGLGIAAWFALPGASAWAAFLLAAAALGLAAAASAAGTRTGKALALFGL
                     AAALGCGLIWWKAERVAAPPLAYPRMATFAATIDSFQRLPAKESVRLVVLPEPGSGLP
                     PKLRINLDAEDASDALQPGAIVRIRSWLMPPPPMAVPGAYDFARYAWFQRIGGTGRAL
                     DVTLVAPARGQSWRARLAGWRQRLADHIQTRLKGGEGGIATALATGDQGGISEEDAEA
                     MRRSGLAHLL"
     gene            857..2314
                     /gene="gltX"
                     /locus_tag="VF589_12980"
     CDS             857..2314
                     /gene="gltX"
                     /locus_tag="VF589_12980"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX8258538.1"
                     /translation="MPASLENRRLSATTETSAVVTRFAPSPTGFLHIGGARTALFNWL
                     FARGRGGKFLLRIEDTDKARSTEAAIAAILDGMRWLGLDWDGHEYYQSQFWARHAEVA
                     NEMLARGHAYRCYMSQDELAEARAAAQRDKQPFRIQSRWRDGCEPPSPDAPFVVRLKA
                     PREGETAIDDKVQGRVSVQNAELDDFVLLRSDGSPTYMLAVVVDDHDMGVTHVIRGDD
                     HLNNAFRQLAIVRAMGWPEPTYAHVPLIHGQDGAKMSKRHGATGVEAYRDELGILPEA
                     LSNYLLRLGWGHGDEEIIAREDAVKWFDLAQVGRSPSRFDMKKLENLNGHYIREASDG
                     RLAGLVAPRVEAEIGRSLTTDELALLTRAMPELKPRAKHLNELADGAAFLFKTVPLDM
                     DERAAALLEGDAAELLALVHFALSRLPEWTPERIEQAVREVAEARAVKLGQVAQPLRA
                     ALTGRTTSPGIFDVLVLLGREESLARIAGHKAKKD"
     gene            2318..3619
                     /locus_tag="VF589_12985"
     CDS             2318..3619
                     /locus_tag="VF589_12985"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX8258539.1"
                     /translation="MADDNLAGEGATLGIGGKEYRYDVLGGTVGPDVVDIRKLYGQTG
                     AFTYDPGFTSTAACESAITYIDGDAGILLHRGYPIDQLAEHSSFMEVAYLLLNGELPK
                     KDDLDSFTRTISRHTMLHEQLATFYRGFRRDAHPMAIMCGVVGALSAFYHDSTDITDP
                     KQRMIASHRLIAKMPTIAAMAYKYSVGQPFMYPDNSLSYTGNFLRMTFGVPAEPYQVN
                     PIVENAMRRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEA
                     ALNMLREIGRPERIPEYIARAKDKEDPFRLMGFGHRVYKNFDPRAKVMKETAEAVLNE
                     LGVTDPVFDVARQLEQIALSDDYFIEKKLYPNVDFYSGVILSAIGFPTSMFTALFALA
                     RTVGWVAQWNEMISDPDQKIGRPRQLYTGPTQRDYVPVAER"
     gene            3729..6206
                     /locus_tag="VF589_12990"
     CDS             3729..6206
                     /locus_tag="VF589_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258540.1"
                     /translation="MDYQGTDGTDVITGTDDIDTINGNGGDDQLFGLGQEDFLYGGAG
                     DDYLDGGEIRDVLTGGNGDDVYIVDLDGVYVPDGDGVAIGYYLPDSVREEAGAGIDEV
                     RTSMSTYSLGEMNFIGPDGVSRRRAGGINVENLTGTSDAGQTLTGNELDNIITGAAGD
                     DFLFGRGGVDTLVGGLGNDRYEVDEEDVIVEAADGGIDHVGMRMSSYTLPSHIENLTG
                     LRRYGGQHLTGNELDNVITGTRGSERLEGLGGADTLVGGGGHDDYYADSADTLIEAVG
                     GGTDRVRTYDSHILGANLENLALLGSAAANGYGNDLRNVIEGNGADNILYGYKGNDAL
                     RGGAGADELFGNDEDDSLRGEGGDDRLNGGNGMDFLEGGAGNDRLLGGADDDLVKDSD
                     GDDVMRGDAGFDTVSYYGATAGVTVNLAITTAQTTGGAGTDILSGFERLIGSNFRNSL
                     TGDAGANTILAADGNDRVEGGDGNDRLYGNDGIDTASYAGASAGVSVSLGLLLQNTRG
                     AGVDRLESFENLIGSQFADRLEGNGEANRLEGGAGNDRLIGGPGDTLVGGAGNDIYFV
                     GGGSLTFDERAGGGHDAVTSSADHVLAAFIEDLRLTTGGLTGTGNGLDNALTGSAGTD
                     ALYGLAGDDRVIGGGGHDSGFGGDGDDLLAGGDGDDSLEGGNDDDRLSGQAGADNLLG
                     GDGSDRLIGAQDGDTLDGGAANDSVDGGAGIDILTGGAGADSFDFDDGDLGATRAGAE
                     RIADFSGYAGDRIDLRSIDADQGTAGDQNFHFIGESGFSGAAGELRFDVVDGNSFVQG
                     DTDGDGAADFFLQLDGTPPLVAVDFVV"
     gene            6322..>7101
                     /locus_tag="VF589_12995"
     CDS             6322..>7101
                     /locus_tag="VF589_12995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8258541.1"
                     /translation="MRAISDGQSTGPHVAVVGGGFSGAMTAYHLLKAGGCRVTLIEQR
                     ERAAAGAAYSTEDEAHVLNVRAANMSALPDAPAHFAEWLERQGGGDGGSFAQRRLYHR
                     YIAELLERQDGGGRLTVVRGTALALSDAGGRLALSMADGGEITADRVVLASGNLPARR
                     PPLAGEPGLIEGRYVADPWSAEGRAAVAGIAGIDGDVLLLGTGLTMVDMCLSLTGAGF
                     GGRIVASSRRGLIPHGHAPFEPRPLAEPPAGRLAELLRETRA"
BASE COUNT         1172 a         2334 c         2554 g         1041 t
ORIGIN      
        1 caggaggtgc gcgaggcccg agcggcgcat cgcctcggca tcctcctcgg aaatgccgcc
       61 ctggtcgccg gtggcgagcg cggtggcgat accgccctcc ccgcccttca gccgcgtctg
      121 gatatggtcg gcgaggcgct gccgccagcc ggcgaggcgg gcgcgccagc tttgcccgcg
      181 cgccggggcg accagcgtca cgtcgagcgc gcggccggtg ccgccgatcc gctggaacca
      241 ggcgtagcgg gcgaaatcat aggcgcccgg cacggccatc ggcggcggcg gcatcagcca
      301 ggagcggatg cggacgatgg cgcccggctg gagcgcgtcg gaggcgtcct cggcatccag
      361 gttgatgcgc agcttgggcg gaaggccgga gccgggctcc ggcaggacga cgaggcggac
      421 cgattccttg gccggcaggc gctggaagct gtcgatagtg gcggcgaagg tggccatgcg
      481 cgggtaagct agcggcgggg ccgcgacccg ctcggccttc caccagatga gcccgcatcc
      541 gagggcggcg gcgaggccga acagggccag cgccttgccg gtgcgggtgc cggcggcgga
      601 agcggcggcg gcgaggccga gcgcggcggc ggcgagcagg aaggcggccc aggcgctggc
      661 gcccggcagc gcaaaccagg cggcgatgcc gagcccgagg ccgactggaa gccacaaggg
      721 aagctggtcg cgctcggcgt cgagccgggc ttcgagccga cccgcgaggg cggcgaagcg
      781 gccgcgcagg ccgcccgatc ggctcgtttg tagcggatca tccgctatgc taggggcgct
      841 cgcacccgtc gtcggcatgc cggcatcctt ggagaatcgg cgcttgagcg caaccacaga
      901 aacgagcgcc gtcgtcacgc gcttcgcgcc ttcgcccacc ggattcctgc atatcggcgg
      961 cgcccggacc gctttgttca actggctgtt cgcgcgcggc cgcggcggca agttcctgct
     1021 gcgcatcgag gataccgaca aggcgcgatc gaccgaggcg gcgatcgcgg cgatcctcga
     1081 cggcatgcgc tggctcggcc tcgactggga cgggcacgaa tattatcagt cgcaattctg
     1141 ggcgcggcac gccgaagtcg ccaacgagat gctggcgcgc ggccacgcct atcgctgcta
     1201 tatgagccag gacgagctcg ccgaggcgcg cgcggcggcg cagcgcgaca aacagccgtt
     1261 caggatccag agccgctggc gcgacggctg cgagccgccc tcccccgacg ccccgttcgt
     1321 cgtgcgcctg aaggcgccgc gcgagggcga gacggcgatc gatgacaagg tccagggccg
     1381 ggtgtcggtg cagaatgccg agctcgacga tttcgtcctg ctccgctccg acggcagccc
     1441 gacctacatg ctcgcggtgg tggtcgacga tcacgacatg ggcgtcaccc acgtcatccg
     1501 cggcgacgat cacctcaaca acgccttccg tcagctggcg atcgtccggg cgatgggctg
     1561 gcccgagccg acctatgccc atgtgccgct gatccacggc caggacggcg ccaagatgtc
     1621 gaagcggcac ggcgccaccg gcgttgaggc ctatcgcgac gagctcggca tcctgcccga
     1681 agccttgtcc aactatttgc tgcggctcgg ctggggccat ggcgacgagg agatcatcgc
     1741 gcgcgaggat gccgtcaaat ggttcgacct cgcccaggtc ggccgctcgc cttcccggtt
     1801 cgacatgaag aagctggaga acctcaacgg ccattatatc cgcgaggcgt cggacgggcg
     1861 gctggccggg ctggtcgcgc cgcgcgtcga ggcggagatt ggccgttcgc tcaccaccga
     1921 cgagctggcg ctgctgaccc gggcgatgcc ggaattgaag ccccgggcca agcatctcaa
     1981 tgaactcgcc gacggcgccg cctttctgtt caagacggtg ccgctcgaca tggacgagcg
     2041 cgccgccgcc ttgctagagg gcgacgcggc cgagctgctc gccctcgtcc atttcgcgct
     2101 gtcgcggctg ccggaatgga cgccggagag gatcgagcag gccgttaggg aggtggctga
     2161 ggcccgggcc gtgaagctcg gccaggtggc gcagccgctg cgggcggcgc tgaccggccg
     2221 gacgacctcg cccggcattt tcgatgttct ggtgctgctc ggccgggagg aaagcctggc
     2281 ccggatcgca ggccacaaag ccaagaagga ttgattgatg gcggacgaca atttggcggg
     2341 cgaaggcgcg accctcggca tcggcggcaa ggaatatcgc tacgacgtgc tcggcggcac
     2401 ggtcggcccc gacgtggtcg acatccgcaa gctctacggc cagaccggcg ccttcaccta
     2461 cgatcccggc ttcacctcga ccgcggcgtg cgagagcgcg atcacctata tcgacggcga
     2521 tgccggcatc ctgctccacc gcggctatcc gatcgaccag ctcgccgagc attcaagctt
     2581 catggaggtc gcctatctgc tgctgaacgg cgagctcccg aagaaggacg atctcgattc
     2641 cttcacccgc accatttccc gccacaccat gctgcacgag caattggcga ccttctaccg
     2701 cggcttccgg cgcgacgcgc atccgatggc gatcatgtgc ggcgtggtcg gcgcgctcag
     2761 cgccttctac cacgattcga ccgacatcac cgatcccaag cagcgcatga tcgccagcca
     2821 ccggctgatc gcgaagatgc cgaccatcgc ggcgatggcc tataaatatt cggtcgggca
     2881 gccgttcatg tatcccgaca acagcctcag ctataccggc aatttcctgc gcatgacgtt
     2941 cggcgtcccg gcggagccct atcaggtcaa cccgatcgtc gagaatgcga tgcggcggat
     3001 cttcattctc cacgccgacc atgagcagaa cgcgtcgacc tcgaccgtgc ggctggcggg
     3061 gtcatcgggc gccaacccgt tcgcctgcat cgccgccggc atcgcctgtt tgtggggccc
     3121 ggcccatggc ggcgccaacg aggccgcatt gaacatgctg cgcgagatcg gccggcccga
     3181 gcgcatcccc gaatatatcg cgcgcgccaa ggacaaggag gacccgttcc ggctgatggg
     3241 cttcggccac cgggtctaca agaatttcga cccgcgcgcc aaggtgatga aggagaccgc
     3301 cgaggcagtg ctgaacgagc tgggcgtcac cgaccccgtg ttcgacgtcg cccgccagct
     3361 cgagcagatc gcgctcagcg acgattactt catcgagaag aagctctatc ccaatgtcga
     3421 tttctattcg ggcgtgatcc tgtcggcgat cggcttcccg acctcgatgt tcaccgcctt
     3481 gttcgcgctc gcgcggacgg tcggctgggt cgcgcagtgg aacgagatga tctccgaccc
     3541 cgaccagaag atcggccgcc cgcgccagct ctacaccggg ccgacgcagc gcgattacgt
     3601 gccggtggca gagcgttagg gttgctccag gccggcagcg acgcgccatc tgcggattcc
     3661 cgcaagactc gcttctttca agggtttagg ctgaggcgct gacaaggact tgagcgggga
     3721 gcgggtggat ggattatcag ggcacggacg ggacggacgt catcaccggc accgatgaca
     3781 tcgacacgat caacggcaat ggcggcgacg accagctgtt cgggctcggc caggaagatt
     3841 ttctctacgg cggcgcgggc gacgactatc tcgacggcgg cgaaatccgc gatgtgctga
     3901 ccggcgggaa tggcgacgac gtttacatcg tcgatctcga cggggtctac gttccggacg
     3961 gcgatggcgt cgcgatcggc tattacctgc ccgactcggt tcgggaggaa gccggcgcgg
     4021 gcatcgacga ggttcgcacc tcgatgtcca cctacagcct cggcgagatg aacttcatcg
     4081 gcccggacgg cgtttcgcgc cgccgcgccg gcgggatcaa cgtcgagaat ctcaccggca
     4141 cgtccgacgc ggggcagacg ctgaccggga acgaactcga caacatcatc accggcgccg
     4201 cgggcgacga tttcctgttc ggcaggggcg gcgtcgacac tctggtcggc ggcctcggca
     4261 acgaccgcta cgaggtcgat gaagaggatg tgatcgtcga ggcggcggac ggcggcatcg
     4321 accatgtggg catgagaatg tcgagctaca cgctgcccag tcacatcgaa aacctcacgg
     4381 ggctgcgccg ttatggaggc cagcatctca ccggcaacga gctcgacaac gtgattaccg
     4441 gcacgagagg ctccgagaga ctcgagggcc tcggcggcgc cgatacgctc gtcggcggcg
     4501 gcgggcacga tgattattat gccgactccg cggatactct catcgaagcg gtcggcgggg
     4561 gtacggatcg ggtaaggacg tacgacagcc acatactcgg tgcgaatctc gagaatctgg
     4621 cgctgctcgg aagcgccgcg gccaacggct atggcaacga tctgcgaaat gtcatcgaag
     4681 gcaatggcgc cgacaacatc ctctacggct ataaggggaa tgacgcattg cgcggcggcg
     4741 cgggcgcgga cgaactgttc ggcaatgacg aggatgacag cctccgcggc gagggtggcg
     4801 acgacaggct caacggcggc aacggcatgg attttctcga gggcggcgcg ggcaatgacc
     4861 gtttgctggg cggcgccgac gacgacctgg tcaaggacag cgacggcgac gatgtgatgc
     4921 gcggcgatgc cgggttcgac acggtgagct attacggggc gacggcgggc gtgacggtga
     4981 accttgccat cactaccgcg cagacgaccg gcggcgccgg caccgacatt ctgagcggct
     5041 tcgagcgact catcggctcc aactttcgca actcgctgac cggcgacgcc ggcgccaata
     5101 cgattctagc cgcggacggc aacgaccggg ttgaaggcgg cgacggcaac gaccggctct
     5161 acggcaatga cggcatcgac acggccagct atgcgggggc ctcggccggg gtatccgtgt
     5221 cgcttggcct gctcttgcag aacacgcgcg gcgcgggtgt ggaccggctg gagtcgttcg
     5281 agaatttgat cgggtcgcag tttgccgatc gcctggaagg gaatggcgaa gccaaccgcc
     5341 tggagggcgg cgccggcaac gatcgcctga ttggaggccc cggcgacacg ctggtggggg
     5401 gcgccggcaa cgacatctac tttgtcggcg gcgggagctt gacgttcgac gagcgagcgg
     5461 gcggaggaca cgacgccgtc accagcagcg ccgaccatgt ccttgcggcc tttatcgagg
     5521 atctgcgcct cacgaccggc gggctgaccg gcaccggcaa cggtctcgac aacgcgctga
     5581 cgggatcggc cggcaccgac gcgctttacg ggctcgccgg cgacgaccgc gtgatcggcg
     5641 gaggcggcca tgacagcggg ttcggcggcg acggcgacga tctcctcgcc ggcggcgacg
     5701 gcgacgacag cctcgagggc gggaacgatg acgaccgcct ctccggccag gccggagccg
     5761 ataatctgct cggcggggac ggcagcgatc gtctgatcgg agcgcaggac ggggacacgc
     5821 tcgacggcgg cgccgccaac gactccgtcg atggcggcgc cggcatcgac atcctgaccg
     5881 gcggcgcggg cgcggacagc ttcgatttcg acgatggcga cctcggcgcg acccgcgcgg
     5941 gagcggagcg gatcgccgac ttcagcggct atgccggcga tcgcatcgac ctgcgctcga
     6001 tcgacgccga tcaaggcacc gcgggcgacc agaatttcca cttcatcggc gagagcggct
     6061 tcagcggcgc ggcgggcgag cttcgcttcg acgtggtcga cggcaatagc ttcgtccagg
     6121 gcgacaccga tggcgacggc gcggccgatt tcttcctgca gctcgacggc accccgccgc
     6181 tggtggcggt cgacttcgtc gtctgagcac tgcgccggcg agcccgcccg gcgaaatacc
     6241 ttggtggaag cgccgcccgc ccgctagggc ggccggcggc ggcgccctga ggcggcgctt
     6301 tcaggcgagc ttcgggacgg ggtgcgggcg atcagcgacg gacaatctac gggcccgcat
     6361 gtggcggtgg tcggcggcgg cttcagcgga gcgatgacgg cctatcacct gctcaaggcc
     6421 ggcggctgcc gcgtgacctt gatcgagcag cgcgagcgag ccgcggccgg agccgcttat
     6481 tcgaccgagg acgaagccca tgtgctgaac gtgcgcgcgg ccaatatgag cgcgctgccc
     6541 gacgcgccgg ctcatttcgc cgagtggctg gagcgccagg gcggcggcga cggggggagc
     6601 ttcgcgcagc ggcggctcta ccacaggtac atcgccgaat tgctggagcg gcaggacggc
     6661 ggcgggcggc tcaccgtggt tcgcggcacc gcgctggcgc tgtcggatgc gggcgggcgg
     6721 ctggcgctgt cgatggcgga cgggggcgag atcacggccg accgggtcgt gctggcgagc
     6781 ggcaatctgc cggcgcggcg gccgccgctc gccggggagc cggggctgat cgagggccgc
     6841 tacgtcgccg atccctggtc ggccgaaggg cgcgcggcgg tggccgggat cgcggggatc
     6901 gacggcgacg tgctgctgct cggcaccggc ctcaccatgg tcgacatgtg cctgtcgctg
     6961 acgggcgccg gctttggcgg acggatcgtg gcgagttcgc ggcgcgggct gatcccgcac
     7021 ggccacgcgc cgttcgagcc gcggccgctg gcggagccgc cggcggggcg gctggccgag
     7081 ctgctgcgcg agacgcgggc g
//
LOCUS       DASVRK010000311         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_57562, whole genome shotgun sequence.
ACCESSION   DASVRK010000311 DASVRK010000000
VERSION     DASVRK010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57562"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VF589_13000"
     CDS             <1..965
                     /locus_tag="VF589_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEX8258542.1"
                     /translation="LNAGGSAYPMGWPGYRACMSYDREYWERVETDYEGGVLTIRVTP
                     ASSSAWFAYFAPYTMERHHDFVAAVAAHPEAQHRSLGQTLDGQELDYFRIGEGPLQAW
                     LYGRQHPGESMTEYWMEGALEKLLDDADPVARVLKQRATFHVVPNMNPDGSRRGHLRT
                     NAVGTNLNREWHDPSPERSPEVLYVRREMDLTGVDFAIDVHGDEAIPANFLAGFEGIP
                     SWTKAKGALYARYSDALVRLSPDFQTAKGYEISPPGKANLSMSTAQLAERYGALSMTL
                     EMPFKDNDDLPDPEQGWSPDRSRALGRSCLDALFAIIDEIPKRG"
     gene            1107..>3195
                     /locus_tag="VF589_13005"
     CDS             1107..>3195
                     /locus_tag="VF589_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258543.1"
                     /translation="MATFNGTPNSDLLYGTDVADEIHGAAGNDDIFGLSGDDVLYGED
                     GNDQLDGGSGGDAMDGGIGNDHYLVDDVNDSVRDADGIDHVRTTLSSYALGATIEKLT
                     GQSADGQSLFGNGLANIITGGAGADVIDGGGGDDTYIVGEGDSVSDSGGGMDHVISSA
                     ASQALAEGIENLTGAANVQQTLTGNDLANIIRAVAENVSWSGASKTLRGGGGDDSLYG
                     AAGQDRLDGGTGADLMVGGSGGDTYIVDDLGDRISETGNYWDTDQVFASVDFMLPDTI
                     ENLTGTSDSGQVLTGNGLKNELRGGAGADTFQGGLGNDIYFVEANDLVIESSPETDTP
                     HDDGTMDEIRTSIQVYTLQDSIEKLTALDDSDKIYTGNFKLNTIVTGAGDDIIDGGAH
                     GDRMFGGAGNDIYYVDEPNERFNTPGGGYDQDYDQVSEYFDKGIDEVRTSFDYFLGDN
                     VENLRARAGSGPLELTGNSLDNVITGGRFDDALVGGLGDDRLIGGRGIDTAKYTTATG
                     GVVVSLLLRDAQDTEAAGIDTLAGIENLSGSAHADILRGNEGHNKLSGGAGDDLLRGF
                     GGNDQLLGGTGVDGVTYVGTESRVVIDLARSDAQNTGGAGIDLLNGIEDVTGSSFGDI
                     LRGSERANRIVGGDGADRLDGKAGADTIIGGAGADLITGGQGADVLTGGTGDDIFTIA
                     RGTTRSDSSPTSID"
BASE COUNT          601 a         1070 c         1059 g          465 t
ORIGIN      
        1 tcttgaatgc cggcggcagc gcctatccga tgggctggcc cggctaccgc gcctgcatgt
       61 cctacgaccg cgaatattgg gagcgggtcg agaccgatta tgaaggcggc gtgctgacca
      121 tccgggtgac gccggcgtcg agcagcgcct ggttcgccta tttcgcgcct tatacgatgg
      181 agcggcacca cgatttcgtc gccgcggtcg ccgcccatcc cgaggcccag caccgctcgc
      241 tcggccagac gctggacggg caggagctcg attatttccg gatcggcgag gggccgctcc
      301 aggcctggct ctacggccgc cagcatcccg gcgagagcat gaccgaatat tggatggaag
      361 gcgcgctcga gaaattgctc gacgatgccg acccggtggc gcgggtgctg aagcagcggg
      421 cgaccttcca cgtcgttccc aacatgaatc cggacggttc gcggcgcggc cacctgcgga
      481 ccaacgcggt cggcaccaat ctcaaccgcg agtggcacga ccccagcccc gagcgcagcc
      541 ccgaagtgct ctacgtgcgg cgcgagatgg acctgaccgg cgtcgacttc gcgatcgacg
      601 tccatggcga cgaggcgatc ccggccaatt tcctcgcggg tttcgagggc ataccgagct
      661 ggaccaaggc caagggcgcg ctctacgccc gctacagcga cgcgctggtg cggctttcgc
      721 ccgacttcca gaccgccaag ggctatgaga tatcgccgcc gggcaaggcc aatctgtcga
      781 tgtcgaccgc gcagctggcc gagcgctacg gcgccttgtc gatgacgctg gaaatgccgt
      841 tcaaggacaa tgacgatttg cccgatcccg agcagggctg gtcgcccgac cgcagccgcg
      901 cgctcggccg gtcgtgcctc gacgccctgt tcgcgatcat cgacgagatc ccgaagcggg
      961 gttagcgggt ccctggccgg cggccgcggg cgccgtcccg ggcgagcagc cccgcgcgag
     1021 gcgtccgcta tacccgcgcc ctaaaatacc gtattcaccg cgtgggatag cctagccgcc
     1081 accccgattc gaggaccgcc cttttcatgg ccacattcaa cggtacgccc aatagcgacc
     1141 tcctctacgg cacggacgtg gccgacgaga tccatggcgc cgccggcaat gacgacattt
     1201 tcggcctgag cggcgacgac gtgctgtacg gggaagatgg caacgaccag ctcgacggcg
     1261 gcagcggcgg cgatgccatg gacggcggca tcggcaacga ccattatctc gtcgatgacg
     1321 tcaacgacag cgtccgcgac gcggacggta tcgatcacgt gcggaccacc ctgtccagct
     1381 atgccctcgg cgccacgatc gagaagctga ccggccagtc cgccgacggc cagtcgctgt
     1441 tcggcaacgg gctcgccaat atcatcaccg gcggggcggg cgccgacgtg atcgacggcg
     1501 gcggcggcga cgacacctac atcgtcggcg aaggcgacag tgtcagcgac agcggcggcg
     1561 gcatggacca cgtgatcagt tcggccgcgt cgcaggcgct cgcggagggc atcgaaaacc
     1621 ttaccggcgc ggccaacgtt cagcagacgc tgacggggaa cgatctcgcc aacatcatcc
     1681 gggcggtcgc cgagaacgtc tcgtggagcg gcgccagcaa gaccttgcgg ggcggaggcg
     1741 gcgacgacag cctgtatggc gcggccggac aggaccggct cgacggcggc accggcgccg
     1801 acctgatggt cggcggcagc ggcggcgaca cctatattgt cgacgatctg ggcgatcgca
     1861 tttcggagac cggcaattat tgggacaccg accaggtctt tgcatcggtg gacttcatgc
     1921 tccccgacac gatcgaaaat ctgaccggca cgtccgattc gggccaggtg ctgaccggca
     1981 atggattgaa gaacgagctg cgcggcggcg ccggcgcgga caccttccag ggcgggctcg
     2041 gcaacgacat atattttgtc gaagccaacg acctggtgat cgaatccagc cccgagaccg
     2101 acacgccgca tgacgacggc accatggatg agatcaggac ctcgatccag gtctatacgc
     2161 ttcaggacag catcgagaag ctgaccgccc tcgacgacag cgacaagatc tataccggca
     2221 acttcaagct aaacaccatc gtcaccggcg cgggcgacga catcatcgac ggcggggccc
     2281 atggcgaccg gatgttcggc ggcgccggca atgacatata ttatgtcgac gaacccaatg
     2341 aaaggttcaa cacgcccggc gggggatatg accaagatta cgatcaggtc tcggagtatt
     2401 tcgacaaggg gatcgacgag gtccgcacct cgttcgacta tttcctcggc gacaatgtcg
     2461 agaatctgag ggcacgcgcc ggcagtgggc cgctcgagct caccggcaac agcctcgaca
     2521 acgtcatcac cggcggccgc ttcgacgacg cgctggtcgg cgggctcggc gacgaccggt
     2581 tgatcggcgg ccgcggcatc gacaccgcca aatatacgac ggccactggg ggcgtcgtgg
     2641 tgagcctgct gctgcgcgac gcgcaggaca ccgaagccgc cggcatcgac acgctggccg
     2701 gcatcgagaa tctctccgga tcggcccatg ccgacatatt gcgcggcaat gagggccaca
     2761 acaaattgtc gggcggcgcc ggcgacgacc tgctgcgcgg cttcggcggc aacgaccagc
     2821 tgctcggcgg caccggagtg gacggcgtca cttatgtggg gaccgagagc cgcgtggtca
     2881 tcgaccttgc ccgcagcgac gcccagaata ccggcggcgc cggcatcgac ctgctgaacg
     2941 gcatcgagga tgtcacgggc tccagcttcg gcgacatcct gcgcggcagc gagcgcgcca
     3001 accgcatcgt cggcggcgac ggggccgatc gcctggatgg aaaagcgggc gccgacacta
     3061 tcatcggcgg cgcgggcgcg gacctcatca ccggcggcca gggcgccgac gtgctgaccg
     3121 gcggcacggg cgacgacatc ttcaccatcg cgcgcggcac gacccgcagc gacagctcgc
     3181 cgacctcgat cgacc
//
LOCUS       DASVRK010000312         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_558468, whole genome shotgun sequence.
ACCESSION   DASVRK010000312 DASVRK010000000
VERSION     DASVRK010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558468"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VF589_13010"
     CDS             complement(<1..338)
                     /locus_tag="VF589_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258544.1"
                     /translation="MDEASRVWKLPEGVPSPSDFADAWGRVFAGTLDLVDATARRTLC
                     PDAPPPYDPAAPVRAFTELGAYFAGHPVEFFKAQQQAATDGMKLWTSAAARAAGAAPE
                     PVIAPARGDRR"
     gene            complement(353..568)
                     /locus_tag="VF589_13015"
     CDS             complement(353..568)
                     /locus_tag="VF589_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258545.1"
                     /translation="MTFEARAAAFVADVETFADFGRPAPRMAALPAHDAVADPEAGWA
                     ARNPERALAFLFVSPRDCAALDELMAA"
     gene            631..1587
                     /locus_tag="VF589_13020"
     CDS             631..1587
                     /locus_tag="VF589_13020"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8258546.1"
                     /translation="MCRYRRAKLHRRAWDRGRALAEFDWNDLRSFLAIARSGRLTAAA
                     ARLRLDHTTLSRRIGALEHALKARLFDRSPSGYTLTEQGNRLLPIAEEMERLSLGAQQ
                     AVGGSAASVEGRVRIGSPEGFGSYFLAPRIAALQDRHPGLVVELVAASAVFSLSKRDA
                     DLVVSVSRPPTGRLKVSKLIDYDLALYAAPAYLAGRAPIATAADLEGHRFVSYIGELL
                     HFPELDFLQHVAPGGATSLESSNLVAQLRATLGGAGLCVLPAFLAGEEAGLVRVLPGE
                     VSLTRSLWLIVHQDLAELARVKAVVRFVREQVEAASASFQLG"
BASE COUNT          252 a          629 c          643 g          271 t
ORIGIN      
        1 cgccggtcgc cgcgggcggg cgcgatcacc ggctccggcg cggcgccggc ggcgcgcgcg
       61 gcggcgctgg tccacagctt catcccgtcg gtcgcggcct gctgctgcgc cttgaagaac
      121 tcgaccggat ggccggcgaa ataggcgccg agctcggtga aggcgcgcac cggagcggcg
      181 ggatcgtaag gcggcggcgc gtcggggcag agcgtgcgcc gggcggtggc gtcgaccagg
      241 tcgagcgtgc cggcgaagac gcggccccag gcgtcggcga aatcggacgg gctcggcacg
      301 ccctccggaa gcttccagac gcggctcgcc tcatccatag tctcgctccc gttcaggcgg
      361 ccatcaactc gtcgagcgcg gcgcaatcgc gcggcgacac gaacagaaag gccagcgccc
      421 gctccgggtt ccgcgcggcc cagccggcct cggggtcggc gacggcgtca tgcgccggaa
      481 gcgccgccat gcggggggcg gggcggccga aatcggcgaa agtctcgacg tccgccacaa
      541 aggcggccgc gcgggcctcg aaggtcatgt ccattcctct tgaaggggtg ccggcgctct
      601 cgcataaccg gcgcggcata gccattgggc ttgtgccgat accgtcgtgc gaaactccac
      661 agacgggcat gggatagggg gcgggcgctg gccgagttcg actggaacga cctcaggagc
      721 ttcctcgcga tcgcgcgcag cgggcggctg acggcggcgg cggcgcggct gcggctcgac
      781 cacaccaccc tctcccgccg catcggcgcg ctcgaacatg cgctgaaggc ccgattgttc
      841 gaccgcagcc cgtccggcta cacgctgacc gagcagggca accggctgct gccgatcgcc
      901 gaggagatgg agcggctgtc gctcggcgcg cagcaggcgg tcggcggcag cgcggcctcg
      961 gtcgaggggc gggtgcggat cggatcgccc gaaggcttcg gcagctattt cctggcgccg
     1021 cgcatcgccg cgctccagga ccgccatccc ggcctggtcg tcgaattggt cgcggcctcg
     1081 gcggtgttca gcctgtcgaa gcgcgacgcc gacctggtcg tctcggtgag ccggccgccg
     1141 accgggcggc tcaaggtcag caagctcatc gattacgacc tcgccctcta cgcggcgccc
     1201 gcctatcttg ccggccgcgc gccgatcgcc accgccgccg atctcgaggg ccatcgcttc
     1261 gtcagctata tcggcgagct gctccatttc cccgagctcg atttcctcca gcatgtcgca
     1321 cccggcgggg cgacctcgct tgaaagcagc aatctcgtcg cccagcttcg cgccacgctc
     1381 ggcggggcgg gcctctgcgt gctgccggcc ttcctcgccg gcgaggaggc ggggctggtg
     1441 cgggtgctgc cgggcgaagt cagcctgacc cgcagcctgt ggctgatcgt ccatcaggat
     1501 ctggccgagc tcgcccgggt gaaagcggtg gtgcggttcg tccgcgaaca ggttgaggcg
     1561 gcaagcgcca gcttccagct cggctgagcc gggagcgaca gagtcttcgc gcactcccgc
     1621 cgagtccagc ggcgaatcgc ggcacgtttc gccaagtctt cgcccgaaaa gttcaagtcg
     1681 ggctcgggtc ggcttccttt cggcacgggc tgcaacgcgg ccgcatcctt aagccatgcc
     1741 ggtggttaac ctctcatccc cgtgcgagcg gaggattttc cactgacttt tgtaa
//
LOCUS       DASVRK010000313         4658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_611508, whole genome shotgun sequence.
ACCESSION   DASVRK010000313 DASVRK010000000
VERSION     DASVRK010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611508"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VF589_13025"
     CDS             complement(<1..495)
                     /locus_tag="VF589_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEX8258547.1"
                     /translation="MSASSLPPLALIAGPTASGKSELALTMAEAAGGTIVNADSAQVY
                     ADLRIITARPGREDEVRAPHRLYGYRDGALPCSAAGWAADARREIAAVHAAGGLPILV
                     GGTGLYINTLLNGIAPVPEIDAGIRAEVRAARVLDNHAALEREDAQAAARLRPTDTTR
                     VARAL"
     gene            494..1372
                     /gene="serB"
                     /locus_tag="VF589_13030"
     CDS             494..1372
                     /gene="serB"
                     /locus_tag="VF589_13030"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX8258548.1"
                     /translation="MFIATLIAAGRLGSGDISVAEDRLREAGVEPMGRSWVDPDSACD
                     ILFSADPAIAAAALTGAPHGIDVVVQGEAGRRKKLLIADMDSTMITVECIDELADYAG
                     LKAEVSEVTERAMRGELDFESALRARVALLKGLDETAIDRCRAERVTLTPGATALVRT
                     MKREGAYCLLVSGGFSRFADPVASEIGFDRAISNSLGVEGGRLSGTVGEPIVGAAAKR
                     EALLDAAAAREIDLADTIAVGDGANDIPMLEAAGLGVAYLAKPKVAAAARARIDHNDL
                     GALLFAQGYARDEWEE"
     gene            complement(1374..1658)
                     /locus_tag="VF589_13035"
     CDS             complement(1374..1658)
                     /locus_tag="VF589_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258549.1"
                     /translation="MASRWRRRRLCVLIAGSPAPAALREVSATGAFLETNARPELGSG
                     VELHHPEAGAISGIVRSLAHDGITIGFNCGEHSVAFALAAISADMSRPAA"
     gene            complement(1795..2769)
                     /locus_tag="VF589_13040"
     CDS             complement(1795..2769)
                     /locus_tag="VF589_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8258550.1"
                     /translation="MHCLDRTIFAACALALFAPVAAHADVKAGVDAWQAGNYAAAIKE
                     WRPLADKGDADAQFNLGQAYKMGRGVPANIKIAGGWFQKAAAQGHEHAQTNYGIILYD
                     TGNRAAALPWLKKGADRGDPRSQYALAVELTNGQIIGQDFARAYALMTRSAAQGMPAA
                     ASSLTKMEAFIPLAERQKGIALARQMERGGAMAAKAPRVAGVPTPVKKAPPAKAPALA
                     KAAPPAKAPPAKPAAAAKPAVQVAAKAAPGGKWRVQLGAYGSPAAAQGQWSTLSRKIG
                     ALGGLQPSYEAAGKFTRLRVGPLANRAAADKLCASAKAAGQACFTVAP"
     gene            2954..3673
                     /locus_tag="VF589_13045"
     CDS             2954..3673
                     /locus_tag="VF589_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8258551.1"
                     /translation="MRVLALSSQKGGSGKTTLSGHLAVQAQLAGAGPVCLIDIDPQGS
                     LADWWNEREAEMPAFAQTTVARLGADLEVLRQQGFRLAVIDTPPAITMAIQSVIQVAE
                     LVVIPTRPSPHDLRAVGATVDLCERAGKPLIFVVNAATPKAKITYEAAVALSQHGTVA
                     PVTLHHRTDFAASMIDGRTVMEIDPRGKSAAEVTELWEYISDRLEKNFRRTVFAAPGG
                     AQLHASAPRAAGGFGRRVVGQ"
     gene            3687..4310
                     /locus_tag="VF589_13050"
     CDS             3687..4310
                     /locus_tag="VF589_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258552.1"
                     /translation="MNTFASLSSGLLARKGGAKPAMRPQGFGQMSGMGLEDLGWNDMG
                     HVASQPALVPDIEEERVPSSIAALTPAPRAVPPVQAEPQPEPQPEPQPELEPVYAPPP
                     VVEQQRAIEARFSEAEEAVEIAPARPEAQVVALPPRSRRAAGAKAKAAFTLRLDEARH
                     LKLRLACAVTRRSAQQIVTQALDEFIASQPELETLAGHVPAKFGKRV"
     gene            4343..>4658
                     /locus_tag="VF589_13055"
     CDS             4343..>4658
                     /locus_tag="VF589_13055"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8258553.1"
                     /translation="MKKNVALKLAASSLVLGVTTIGCTNAGTPGPVRLAKAPAAEAGA
                     AQHSAAAQQALQAGNVAAALVSAEKAVELAPSDIGYRMLLADLYLKNGRFKSAEASFR
                     DVL"
BASE COUNT          700 a         1662 c         1600 g          696 t
ORIGIN      
        1 gagcgcgcgc gccacccggg tcgtgtcggt cgggcgaagg cgggcggccg cttgcgcatc
       61 ctcccgctcc agcgctgcat gattatcgag gaccctcgcg gcgcggacct cggcgcgaat
      121 gcctgcgtcg atctccggca cgggagcgat gccgttgagc aaggtgttga tgtagaggcc
      181 ggtgccgccg accaggatcg ggagccctcc ggcggcgtga accgccgcta tctcgcgccg
      241 cgcgtccgcc gcccagccgg cggccgagca gggcagggcg ccgtcgcgat agccgtagag
      301 gcggtgcggc gcccggacct cgtcttcccg gccgggccgg gcggtgatga tgcgcagatc
      361 cgcatagacc tgcgcgctgt cggcgttgac gatcgtgccc cccgccgcct ccgccatggt
      421 gagcgcaagc tccgacttgc cgctcgcggt cggccccgcg ataagcgcca gcggtggcag
      481 cgaagaggcg gacatgttca tcgcgaccct gatagcagcc ggacggcttg gaagcggcga
      541 tatttctgtc gccgaggacc ggctgcgcga agccggcgtc gagccgatgg gccgcagctg
      601 ggtcgatccc gacagcgcct gcgacatcct cttctccgcc gatcccgcca tcgcggccgc
      661 cgctctgacc ggggcgccgc acggtatcga cgtcgtcgtc cagggcgagg ccggccgccg
      721 caagaagctg ctcatcgccg acatggattc gaccatgatc acggtcgaat gcatcgacga
      781 gcttgccgat tatgccggcc tcaaggccga ggtgtccgaa gtcaccgagc gcgcgatgcg
      841 cggcgagctc gacttcgaat cggcgctccg ggcgcgcgtg gctttgctga aggggctgga
      901 cgagaccgcg atcgaccgct gccgggccga gcgcgtcacg cttaccccgg gcgcgacggc
      961 cctggttcgg accatgaaac gcgagggcgc ttattgcctg ctggtctcgg gcggcttctc
     1021 gcgcttcgcc gatccggtcg cgagcgagat cggcttcgac cgcgcgattt cgaattcgtt
     1081 gggcgtcgag ggcggccggc tcagcggcac cgtcggcgag ccgatcgtcg gcgcggccgc
     1141 gaaaagggag gccttgctcg acgccgccgc cgcgcgcgag atcgacctcg cggacacgat
     1201 tgcggtcggc gacggggcca acgacatccc gatgctcgaa gcggcgggcc tcggcgtcgc
     1261 ttatcttgcc aagccgaagg tggccgccgc ggcacgcgcc cggatcgacc ataacgatct
     1321 cggcgctttg ctgttcgccc agggctatgc ccgggacgaa tgggaggaat gaatcaggcg
     1381 gccgggcggc tcatgtcggc gctgatcgcg gccagcgcga aggcgaccga atgttcgccg
     1441 caattgaatc cgatggtgat tccgtcatgg gcgagcgagc gcacgatgcc gctgatcgcc
     1501 cccgcttccg ggtggtgaag ctcgacgccg ctgccgagct cgggccgcgc attggtctcg
     1561 agaaaggcgc cggtggcgga gacttcgcgc agcgccgccg gggcgggcga gcccgcgatc
     1621 agcacgcaca gcctgcgcct gcgccagcgc gaagccatag ccgatgcgcc aaccaacttc
     1681 cgccgtgccg ccatcccgca tcccctgccc aaatcaggga cgatcatagg ccgcaccccg
     1741 caaagaaacc gttaagcgcg aaccttttcc tcgccagcgc tccggccggg agacttacgg
     1801 cgcgacggtg aagcaggcct gtccggcggc cttggccgaa gcgcacagct tgtcggccgc
     1861 ggcgcggttg gcgagcgggc cgacccggag ccgggtgaac ttgccggcgg cttcgtagga
     1921 cggctggagg ccgccaagcg cgccgatctt gcggctgagc gtgctccatt gtccctgcgc
     1981 cgccgccggg ctgccatagg cgccgagctg cacgcgccac ttgccgccag gcgcggcctt
     2041 cgccgccacc tgaacggccg gcttggctgc cgccgcaggt ttggccgggg gcgccttggc
     2101 cggcggagcc gccttggcga gcgccggggc cttggcgggc ggagccttct tgacgggggt
     2161 ggggacgccc gcgacgcgcg gcgccttggc ggccatggcg ccgccgcgct ccatctgccg
     2221 ggcgagggcg atgccctttt gccgttcggc cagcgggatg aacgcctcca tcttggtgag
     2281 gctcgacgcc gccgccggca tgccctgggc cgccgaacgc gtcatcagcg cgtaggcgcg
     2341 cgcgaagtcc tggccgatga tctggccgtt ggtgagctcg accgccagcg catattgcga
     2401 gcgcggatcg ccgcggtcgg cccccttctt aagccacggc agcgccgcgg cgcgattgcc
     2461 ggtatcgtag aggatgatgc cgtaattggt ctgggcatgt tcatggccct gcgccgccgc
     2521 cttctggaac cagccgccgg cgatcttgat attggccggc acgccgcggc ccatcttata
     2581 ggcctggccg aggttgaact gcgcgtccgc atcgcccttg tcggcgagcg ggcgccattc
     2641 cttgatggcg gcggcataat tgcccgcctg ccaggcatcg acgccggcct tgacgtccgc
     2701 gtgcgccgcg accggcgcga acagcgccag cgcgcaggcc gcaaatatag tgcgatccag
     2761 acaatgcatt cagtcctccc cctcaagcgc gaaagcgtaa acccaaaccg cttagcccga
     2821 agcggttaaa gcggtgttta cattaaactc tcggccttcg ccacggccgg gcttgcccag
     2881 tccggggtta accagatttt agcagcctgc ggtgcatcgc tgcccctcaa gcttcctgag
     2941 ccgaggggaa aacatgcgcg ttctggcact gtcgtcgcaa aaaggtgggt cgggcaagac
     3001 caccctgtcc ggccatctgg ccgtacaggc acagcttgcc ggggccggac cggtttgcct
     3061 tatcgacatc gaccctcaag gatcgctcgc cgattggtgg aacgagcgcg aggcggagat
     3121 gccggccttc gcgcagacca cggtcgcgcg gctcggcgcc gacctcgaag tgctgcgcca
     3181 gcagggcttc cggctggccg tcatcgatac gccgccggcc atcaccatgg ccattcagag
     3241 cgtcatccag gtcgccgaac tggtcgtcat cccgacccgc ccgagcccgc acgacctccg
     3301 cgccgtcggc gccaccgtcg atctgtgcga gcgcgccggc aagccgctca tcttcgtcgt
     3361 caacgcggcg acgcccaagg ccaagatcac ctatgaggcc gccgtcgcgc tgtcgcagca
     3421 cggcaccgtc gctccggtca cgctccacca ccgcaccgat ttcgccgcct cgatgatcga
     3481 cggccgcacg gtcatggaga tcgatccccg gggcaagtcg gccgccgaag tgaccgagct
     3541 gtgggaatat atttccgacc ggctcgaaaa gaatttccgc cgcaccgtct tcgccgctcc
     3601 cggcggggcg cagcttcatg cctcggctcc gcgcgccgcg ggcggcttcg gccgccgcgt
     3661 ggtcggtcag tgaggggagg gcactcatga acactttcgc ttccctttcc tccggcctgc
     3721 tcgcccgcaa gggcggcgcc aagccggcca tgcgcccgca gggcttcggc cagatgagcg
     3781 gcatgggcct cgaggatctc ggctggaacg acatgggcca tgtggcctcg cagccggcgc
     3841 tcgtccccga cattgaggaa gagcgcgtgc cgagctcgat cgcggcgctg acgccggccc
     3901 cgcgtgcggt gccgccggtc caggccgaac cgcagcccga gccgcagccc gagccccaac
     3961 ccgagctgga gccggtctac gcgccgccgc cggtggtcga acagcaacgc gcgatcgaag
     4021 cccgcttcag cgaagccgag gaggcggtcg agatcgcccc cgcccggccc gaggcgcaag
     4081 tggtggcgct gccgccgcgt tcccgccgtg ccgcgggcgc caaggccaag gcggcgttca
     4141 cgctccgcct cgacgaggcg cggcatctga agctgcggct ggcctgcgcg gtgacgcgcc
     4201 gctcggcgca gcagatcgtc acccaggcgc tcgatgaatt tatcgcctcg caacccgagc
     4261 tcgaaacgct tgccgggcac gtgcccgcca agttcgggaa gcgcgtttga ggcgtcctga
     4321 aaaaccacaa ggggtgggca agatgaagaa gaatgtcgca ttgaagctgg cggcctcgag
     4381 cctcgtcctc ggcgtcacca cgatcggttg caccaacgcc ggcacgcccg gcccggtccg
     4441 gctcgccaag gcgcccgccg ccgaagccgg cgccgcccag cattccgccg ccgcgcagca
     4501 ggcgctccag gcgggcaacg tcgccgccgc gctcgtctcg gccgaaaagg ccgtcgagct
     4561 cgcgccgagc gacatcggct accgcatgtt gctcgccgac ctctatctca agaacggccg
     4621 cttcaagtcc gccgaggcga gcttccgcga cgtgctga
//
LOCUS       DASVRK010000314         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_60207, whole genome shotgun sequence.
ACCESSION   DASVRK010000314 DASVRK010000000
VERSION     DASVRK010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60207"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2068)
                     /locus_tag="VF589_13060"
     CDS             complement(<1..2068)
                     /locus_tag="VF589_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258554.1"
                     /translation="MAAVIDLSALGTRGFVIQGDQSADLAGSDVALAGDVNKDGFDDI
                     IVSAPFGDSGGEDAGEVYVVFGKAGGFGTVDLSSLTAAQGFAINGDGLNDYAGQSVAG
                     AGDLNGDGFADIVIGAAKNDDGGDGAGAAYVIFGKASGFATVDLGALAAGEGFTITGD
                     EIQDRAGHSVSSAGDVNGDGIDDLIVGAPGSDDGGASAGQAYVIYGKSAGLGNVDLGA
                     LTPADGFALIGDADGDDAGWSVASAGDVNGDGVGDIIMSAYYGNDGGGKSGESYVIFG
                     KPGGHDTIDLSALSSGDGFKIVGSGYSYTGPTPYTKVYFGADRVSGGSDINGDGFDDL
                     LVGSPRSTEYGDGTVSVIFGKAGGFGTIHLGQLSYNQGIHVYGGQDGGFDMSAAGDVN
                     GDGFDDFIVGAPGADDGGIDAGNAYVIYGREDPDWVSLRSLGPTEGFLIQGDQGGDMA
                     GRSVSGGGDVNGDGFADIVVGAPYGDDGGASAGEAYVIFGAAPTTGVTRIGSAIGQTI
                     RGGIGDDRLDGRDGDDLLLAAGGDDELLGAAGNDDLNGGEGDDALSGGSGHDKLTGGA
                     GIDSLTGGSGNDMLEGGSGGDSMNGGSGNDVYRVDSMADQIVETDGGGSDRLFARVSY
                     TLAEGVYVEALNTLSNSGTTVINLIGNSFANKIIGNAGINTLSGGGGADSLNGLAGND
                     RIYGGAG"
     gene            complement(2281..>3997)
                     /locus_tag="VF589_13065"
     CDS             complement(2281..>3997)
                     /locus_tag="VF589_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258555.1"
                     /translation="DTYVLADINKASEFAIAAYDQVTEAAGEGNDTIVVEALDNPDTH
                     SSIERYTLGANVENGLVTGGLAFNLGGNELGNGLTGNAAGNLLAGLAGNDLLHGQGGI
                     DGLAGGLGDDTYVLADINKAGEFGIAAYDETTEAAGEGNDTVLVEALDNPDTHSSIEH
                     YALAANIENGIVTGALAFNLTGNELRNRLTGNGSGNMLSGQDGNDMLSGLGGIDVLIG
                     GAGNDRYVLADINKASAFATAAYDSAIEQGGGGIDTVLVEALDNPDTFSGVDGYTLGL
                     NIEHCIVTGAIAFDLAGNELGNRLTGNAGANRLDGNGGNDRLAGLGGNDIYIVDSARD
                     GIAEAAGGGADRVLASVGYSLAAGVHIEALSAAVAAATTAMNLNGNQVANRITGNAGE
                     NGLRGNAGDDILVGLAGADRLYGGAGNDVLSGGAGRDGFFFQTALDPAANVDRIADFA
                     SADDTIWLDRAIFTALAEGPLVPGAFRPGTAAADASDRIVYDRVTGSIFYDADGAGGA
                     AQILFARVDPGTALSRADFAAYQGPAQAAAEAKPPAFSPELTMITPSRAAAAAAAEWI
                     DVAIV"
BASE COUNT          572 a         1404 c         1316 g          705 t
ORIGIN      
        1 tgcccgcgcc gccgtagatc cggtcgttcc ccgccagccc gttcagcgaa tcggcgccgc
       61 cgccgccgct cagcgtgttg atgccggcat tgccgatgat cttgttggcg aaggaattgc
      121 cgatgaggtt gatcacggtg gtgccgctgt tggagagggt gttcagcgcc tcgacataga
      181 cgccttcggc gagcgtatag ctcacccgcg cgaacaggcg atcggagccg ccgccgtccg
      241 tctccacgat ttgatccgcc atgctgtcga cccggtagac gtcgttgccc gagcctccat
      301 tcatcgagtc tccgcccgaa ccgccctcga gcatgtcgtt gcccgagccg ccggtcaggc
      361 tgtcgatccc ggctcccccg gtcagcttgt catggcccga gccgccgctc agcgcatcgt
      421 cgccctcgcc gccgttgagg tcgtcattgc cggccgcgcc gagcagttcg tcgtcgccgc
      481 cggcggcgag caggaggtcg tcgccgtcgc gcccgtcgag ccggtcgtcg ccgatgccgc
      541 cgcgaatggt ctggccgatg gcgctgccga tccgggtgac gcccgtggtc ggcgcggcgc
      601 cgaagatcac ataggcttcg ccggcgctgg cgccgccgtc gtcgccatag ggcgcgccga
      661 cgacgatgtc ggcaaagccg tcgccgttga cgtcaccgcc acccgagacg ctgcggccgg
      721 ccatgtcgcc gccctgatcg ccctggatca ggaaaccctc ggtcggaccc aggctcctga
      781 ggctgaccca gtccggatcc tcgcggccgt agatcacata ggcgttgccg gcgtcgatcc
      841 cgccgtcgtc ggcgcccggg gcgccgacga tgaaatcgtc gaagccgtcg ccattgacgt
      901 cgccggccgc cgacatgtcg aagccaccat cctgcccgcc atagacgtgg atgccttgat
      961 tgtagctaag ctggccgagg tggatcgtgc cgaacccccc ggctttgccg aagatcaccg
     1021 acacggttcc gtcgccatac tctgtcgagc gcggcgagcc gaccagcagg tcgtcaaagc
     1081 cgtcgccgtt gatgtcgctc ccgccggaga cgcgatccgc gccgaaatag actttcgtgt
     1141 acggcgtagg gcccgtgtag ctatagccgc tgccgactat cttgaatccg tcgccgctgc
     1201 tgagcgcgct gaggtcgatc gtgtcgtggc cccccggctt gccgaagatc acatagcttt
     1261 cgcccgattt gccgccgccg tcgttgccat aataagcgct catgatgata tcgccgacgc
     1321 cgtcgccgtt gacgtccccg gcggaggcga cgctccagcc ggcatcgtcg ccgtcggcgt
     1381 cgccgatcag cgcgaagccg tcggccggcg tcagcgcgcc gagatcgacg ttcccgagcc
     1441 cggcgctctt gccgtagatc acataggcct ggccggcact cgcgccgccg tcgtcgctgc
     1501 ctggagcgcc gacgatgagg tcgtcgatcc cgtcgccgtt gacgtcgccg gccgaggaca
     1561 cgctgtgccc ggcacggtcc tggatctcgt cgccggttat cgtgaaccct tcccccgccg
     1621 ccagcgcgcc gagatcgacg gtggcgaacc cgctcgcctt gccgaagatc acataagccg
     1681 cgccggcgcc gtcgccgcca tcgtcgttct tggccgcgcc gatgacgatg tcggcgaaac
     1741 catcgccgtt gaggtcgccc gccccggcga cgctctgccc cgcataatcg ttcagcccgt
     1801 cgccattgat ggcaaagccc tgcgcagcgg tcaggctgga aagatcgacc gtaccgaagc
     1861 cgccggcctt gccgaagacc acataaactt cacccgcatc ctcgccgccg gaatcgccga
     1921 acggggcgct gacgatgatg tcgtcgaacc catccttgtt gacgtcgccg gcaagcgcca
     1981 cgtccgagcc ggccaagtcg gctgactgat ccccctggat tacgaacccg cgcgttccga
     2041 gcgccgaaag gtcgataaca gctgccacga ctcgcctccc ccactgtcag tgcgcggcaa
     2101 gtctatgcct cggcggccac agttgcgagt ccggacttgc tgatctgtgc caagtagatg
     2161 atctctatta ttcagccgcc accttgaccg gcgcgacgac cgccgacgag tctacttttt
     2221 cgtaactacg cgttctaaag tattgggcca gttagctgag gtcgcacctg cctggccggt
     2281 tcagacgatc gccacgtcaa tccactcggc cgcggccgct gccgctgccc gcgagggggt
     2341 gatcatggtc agctccgggg aaaaggcggg cggcttcgcc tcggccgccg cttgcgccgg
     2401 gccctgataa gcggcgaagt cggcccggct cagcgccgtc cccgggtcca cccgcgcgaa
     2461 caggatctgg gcggcgccgc cggcgccgtc ggcatcgtag aagatgctgc cggtgacgcg
     2521 gtcatagacg atgcggtcgg acgcgtcggc ggcggccgtg ccgggccgga aggcgcccgg
     2581 cacgagcggc ccctccgcca gcgccgtgaa aatcgcccgg tcgagccaga tcgtgtcgtc
     2641 ggccgaggcg aagtcagcga tcctgtcgac gttcgcggcc ggatcgagcg cggtctggaa
     2701 gaagaagccg tccctgccgg ccccgccgct cagcacgtcg ttcccggccc cgccgtacag
     2761 ccggtcggcg cccgccaggc ccaccaggat gtcgtcgccg gcattgccgc gcaagccgtt
     2821 ctccccggca ttgccggtga tgcggttggc gacctggttg ccgttgagat tcatcgcggt
     2881 cgtggcggcc gcgaccgccg cgctcagcgc ctcgatatga actcccgcgg cgaggctgta
     2941 gccgacgctg gcgagcacgc ggtcggcgcc gccgccggcc gcttccgcga tcccgtcgcg
     3001 ggcgctatcg acgatataga tgtcgttgcc gccgagcccc gccagccggt cgttcccgcc
     3061 attgccgtcg agccggttgg cgccggcatt gccggtgagg cggttgccca gttcgttgcc
     3121 ggcaagatcg aaggcgatcg cgccggtgac gatgcaatgc tcgatattga ggccgagcgt
     3181 atagccgtcg accccggaga acgtgtcggg attgtcgagc gcttccacca gcaccgtgtc
     3241 gatcccgccg ccgccctgct cgatggccga atcataagcg gcggtcgcga aggcgctcgc
     3301 cttgttgatg tccgccagca catagcggtc gttgccggcg ccgccgatca gcacgtcgat
     3361 cccgccgagc ccgctgagca tgtcgttgcc gtcctggcct gacagcatgt tgccgctgcc
     3421 gttgccggtg agccggttcc tgagttcgtt gccggtgagg ttgaaggcga gcgcgccggt
     3481 aacgattcca ttctcgatat tggcggcgag cgcataatgt tcgatgctgg aatgggtgtc
     3541 gggattgtcg agcgcctcga cgagcaccgt atcgttgccc tcgcccgccg cttccgtggt
     3601 ctcgtcgtag gcggcgatcc cgaattcgcc cgccttgttg atgtccgcca gcacgtacgt
     3661 gtcgtcgccg aggccgccgg cgaggccgtc gatcccgcct tgcccatgca gcaggtcgtt
     3721 gccggcgagg ccggcgagga ggttgccggc ggcgttgccg gtgaggccgt tgccgagctc
     3781 gttcccgccg aggttgaagg cgagaccgcc ggtgacgagg ccgttctcga cattggcgcc
     3841 gagcgtgtag cgctcgatgc tggagtgggt gtcgggattg tcgagcgctt cgacgacgat
     3901 cgtgtcgttg ccctcgccgg ccgcttccgt gacctggtcg taggcggcga tggcgaactc
     3961 gctcgccttg ttgatgtcgg ccagcacata ggtgtcg
//
LOCUS       DASVRK010000315        18510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_559938, whole genome shotgun sequence.
ACCESSION   DASVRK010000315 DASVRK010000000
VERSION     DASVRK010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 18510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18510
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559938"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VF589_13070"
     CDS             complement(<1..707)
                     /locus_tag="VF589_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8258556.1"
                     /translation="MLAALAALAGCIAPAGRQPPPASYANPVLDGDFPDPAILQAPGG
                     SYYAYATQTERGGKMINLQVARSDDLVDWKLLGDALPVKPVWASKTQDFWAPHVHRAG
                     DRYFLYYSAKPDAALADPSRGLCLAVATATTPEGPFTDSGRPLLCGPGFVNIDPMAYD
                     DPATGKRLLYWGSGFQPIKVQELAPDRISFAPGSEPVDLLHPVKSEDPDEYQRLIEGA
                     WVIRRAGYYYLFYSGDN"
     gene            829..2223
                     /locus_tag="VF589_13075"
     CDS             829..2223
                     /locus_tag="VF589_13075"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8258557.1"
                     /translation="MTSSEPLLSFAVEAAAEGEATRLSVHASDGTARDYRIRGGEHPD
                     YIDFFAELARDFGTRVPGSFEAPAAAPSEVAWRPLITQNLSPRTLSGYGDPAVLKVDG
                     GYILTATSNDAPDAFPILFSADLQHWDHRGFVFPEGATPEWAAAGWHVADFWAPEMAR
                     VGEEYWIAYTARESSNALAIGLARAPSPDGPWVDNGAPLITGGKVLYPGTAASGGVID
                     SHIFVDHDGTPFLFWKNDTNGLWPRPLAGLLREHPALIPSLFDSEADRRSAAFAAAVQ
                     PWANTRRPMERFFLMQPLIEAALDNWARVKPALVGFGHAGDIVEAMRTPIHARRLAPD
                     GRSLIGEEKIVLANDLDWEGHLIEGPFVTRQQGRYWMFYAGNDFSTPAYGIGVAVADH
                     PLGPYVKREEPLLKSHRLWWAPGHASVAPGLDGKPQLFFHAFFPHQCGYKAFRALLTV
                     GLEFSGDEVRLRAI"
     gene            2595..3641
                     /locus_tag="VF589_13080"
     CDS             2595..3641
                     /locus_tag="VF589_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8258558.1"
                     /translation="MSPPLLRRCLAALLASLSAIAPAASEPSFVPVFETNFPDPHVVQ
                     HGGEYIAYATNAGINLPMATSRDLVRWSLVMDPARPKQRLDGMPTLAPWVKEGFTWAP
                     EVMKVGERWLLYYTANHRKRDVQCIGLAVADNPKGPFRDASANPLICQFELGGTIDAN
                     PFRDADGKLYLYYKSDGNRVGKGTAIWGQRLAPDGLSVVGAPAPLVKDDKPWEMKLVE
                     APAMVRSPTGYQLFFAAAYYGWNAEDRLSPYATGYATCSGPLGPCVDAPENPILHSFR
                     EREAGCLSGPGHPSIFEARGRSFIAFHAWAATKGCRKSEDSRYLYIAPIFWEQGKPRI
                     APSLRPAAKLGERR"
     gene            3638..4876
                     /locus_tag="VF589_13085"
     CDS             3638..4876
                     /locus_tag="VF589_13085"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX8258559.1"
                     /translation="MRLAALALCLGTACPAAAQETDDLYRYRAGEETRWVSPENPTGA
                     KGAGAAENRGAKGHAFETIAIGRSHVLADIKGAGIIDRMWMTIEDRSPDALRGLKLEI
                     FWDRAATPAVSAPLGDFFLHGAGEMVAMETALLTSPEGRSFVSYIPMPFRKRARIVVT
                     NESAKPVNLIFYDVNYRALRRQPDDALYFHAWWSRDRATTLGRDFRILPRIAGRGRFL
                     GASVTVLTNPVYEKTWWGEGEVKIALDGDRSGRPTLVGTGAEDYIGTAWGQGAYINRF
                     QGAPIASWDNDGRWTFYRFHVPDPIWFRHDIEVSLQQIGGARKAVVLGLQKKGAPLIP
                     VTIDPGSRTNFQQLLTRDPPVPLSDPSLPDGHTNFYRSDDVAAVAYFYLDRPERALPP
                     IAAPAERRAGLRMAPPPQAK"
     gene            complement(5174..7942)
                     /locus_tag="VF589_13090"
     CDS             complement(5174..7942)
                     /locus_tag="VF589_13090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8258560.1"
                     /translation="MRFHRVFSTTSSLAVLALASTAAAAQVDPAAPVDPTVQAQESPG
                     DPEQGAPQTDDAVQTVDGADQTVAGEEEIVVTGLRRSLQSAQNIKRNSEQIVDSIVAE
                     DIGKLPDLAVSDTAARIPGVQVIRTGGEASTVLIRGLPDFATTYNGREIFTAETRVVA
                     LQDFPSANIAALEVFKTTTPSLVEAGLAGLVNVRSRRPFDFKGLEVAGSVWGLYTAQA
                     GEITPNGNILISNRWDTGIGEIGFLVNASYTQLKFLDSEPSNTDFVADPEINGERVRF
                     PDIQRLFFREGDRSRPSANVALQWRPNDTLEFYIDGLYQGFRNRISDRLVAVPLYGGG
                     SYTNLVFREGTNLLESGTVTGLGDPIFTFQGGTFNKTDTYQFAIGGIYESGPFKLTAD
                     AARTSSKFTGSTESVDRIFAGRENTTVDFDLTTPQFTIRGFDATNPDNFLFDGLYEEN
                     QVSKGDDYQLRADAEYEFQSDFFIRSLQAGVRHTNRDAIREFGNRFAGFRGQDIPGTA
                     LPLEFELARRGFRGTDVQSGFETFVTPTYDSIRENIVALRQFVIDRGATNYTLEDVER
                     VRLYTANEKTLGGYGQINYQFGESLDGVIGLRVVGTKSEFEDINESIGGETGQKYTDF
                     LPTANLRWRITPQLQARLSYTQTRTRPSFAQLNPAVTLGAPDPLQGGLRIGNGGNPNL
                     RPIESNNYDLSLEYFFSRTGFASLAVFRRDLKGFIQDQTLPDFNDPALGPVRITVPVN
                     TRSGRIDGLEAQVTTFFEFGGVPDFLRNFGVQANYTYLDGKVEIFNPLTGEFISDQIT
                     SSGDPDTGGVSKHTFNIAALYEGGPFSARLTYNKRSRYLDRRDVRGDEEGGFYREIAR
                     PAGRLDFSTSYHINDNFTVFFDATNLTGDAFRVDFSSARAGAPRADFVRFLRYDEQTF
                     SLGARFRL"
     gene            8162..9262
                     /locus_tag="VF589_13095"
     CDS             8162..9262
                     /locus_tag="VF589_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8258561.1"
                     /translation="MASLELIDIAKAYGSTAVLDGVSLTMEPREFVAFLGPSGSGKST
                     LLRIIAGLETLDAGEVRLDGRRIDGLPPGDRGVAMVFQHYALYPHMSVRDNMAFGLRN
                     AKVPTPEIEARVAEAARILEIGEHLDKKPGQMSGGQRQRVAIGRAIVKHPKLFLLDEP
                     LSNLDAALRMRTRVELAQLRQRVDAALIMVTHDQAEAMTLADRIVVMNDRRIQQVGPP
                     MEIYSRPANTFVARFVGSPAMTLAPVDLLPGDGPYATVRMGNGAEIATRVPRSGLPAG
                     GALQLGLRPESVKVAANGAIAAEAKVDLVERLGDRTLVYATLADGLAVTAEDEGNSRV
                     RLGDTIGLAIDGAAAHLFDAEGRGHHAEARPA"
     gene            9259..10146
                     /locus_tag="VF589_13100"
     CDS             9259..10146
                     /locus_tag="VF589_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8258562.1"
                     /translation="MSASGGRRDYSGLLFVLPFLTAYLVILIYPLLSGMALSLQRVDL
                     FGGGRFVGFDNYARLFADDSFVRSILNTFALALMIVPLLTMLALGLALALNRATRGAA
                     VFRGIFFSSSVLSVTIVTLIWRFVLAPDAGLLGETAQAIGAEPIPFLSHPTWSLWALA
                     ITTIWWSIGLPMMLFLAGLQQIPADIYEAAALDRASRWTTLRRVTLPSLRRTVVLVVM
                     LQTAAQLQIFGQAQLLTGGGPGGATRTMVLFIFESAFARWELGYATAAAEILFLLIVL
                     LTLTQYVTTMRGSEGDGGR"
     gene            10136..10972
                     /locus_tag="VF589_13105"
     CDS             10136..10972
                     /locus_tag="VF589_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8258563.1"
                     /translation="MAAEASRTRWSPLTLLAILLAAFVMLAPLVWTLLLSLKENEALI
                     GNSGAALSPPYTFENYRSILQGNQVFRWLVNSIIVSGGTTLGVLALCSLAGYGFARLD
                     FPFRRTLFVFVLLGLAIPEQAVILPQHQLFADLRLHNSYPGLILPGLAGPFGVFFMTQ
                     YMRAIPKELDEAAMLDGASRFTLFWKVLLPLTLPAQATLGVFTFLASWNAYWWPLISA
                     TRSDMYTLTVGLAAAQMNYAQTSGLGFLMAQAVFASIPIFIVYLLFQKQIVRAMAGTA
                     VR"
     gene            10969..12282
                     /locus_tag="VF589_13110"
     CDS             10969..12282
                     /locus_tag="VF589_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX8258564.1"
                     /translation="MRRLFILAAACLAGSCSGDAGGGDRAEIYLQRFFGECGAEYGGA
                     TDVSKVEGECGIVTAMINKFNARNPDVHVSSNVVAWPGYPQLSAQMAARDPPDLVTMH
                     TGVISDYSAEGLLEPVEPYLAEAGLSPSALTEASRSAVTIGGRLYGLPFDTHGGLFHI
                     NTKLFAEAGLIKDGKPVLPTSPQEMLAHARQFTERTGKPYLIQSLVGDNAYAARNVYT
                     YLLAQNAVLFPTPRSIRLQTPEAERIVDFFRQITASGVSTRNQDTPAAIASFMSGEGG
                     IYPTGTWMIGQFEAEANKPGRPLYRSYTVYPYPRLWGENVNYVSGHAWVVPKRERTPE
                     QRRAIARFFRFIAEHNFDWARTGHLPAFTAVVDSPAFRALPHRANIAPIARNGRPLPG
                     EVQRQNAIEGLVGEEVAAAFNGQKSTRQALADAERRVNAVLAGIA"
     gene            complement(12254..13525)
                     /locus_tag="VF589_13115"
     CDS             complement(12254..13525)
                     /locus_tag="VF589_13115"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX8258565.1"
                     /translation="MSETRTVPEPTLMAPAEERLHLIDTLRAIALFGVITMNITGMVA
                     ALADDRIFPAATSLDLAFASFDLIVLQGKARSCFAFLFGVGFGILMARAATKGSNFVG
                     FYLARMTALLAIGLCNLAFLFWGDILILYALLGIAMLAFRNASDRLVLSLGLALILLP
                     PVVFGAIEAATGAPMPSLAGLGPDAADEAMAALEPVYSRGSYAEFVQANLQLYFDGYR
                     TDTAHAVTYDVGVLGLFLLGLWAARGGLFADVERWRPLFRKLAWLCLPLGLLLSLIHA
                     TRWMGIEADGAAYGLVTAAYAGLPIAAFGYIAILTLWLSRGGAGVQRALSPMGRMALT
                     GYLASNAAGAFIWYGWGLGQLGAWNFAAMNLLALILFAALCLFSAAWIRRFRFGPFEW
                     LWRSATYGKLQPMRRSAAGLPPARRSPPARR"
     gene            complement(13585..14712)
                     /locus_tag="VF589_13120"
     CDS             complement(13585..14712)
                     /locus_tag="VF589_13120"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8258566.1"
                     /translation="MNRLRGMLADRALRPVLVIWAFAYLLADITVTAMGRMGPGVSTL
                     VSIPLFVLGITFTMLLEGLRQRLRLRPRLIAWAGLGVAVLAATAIFSFADLYYFKWLS
                     LTFVPDWQRWAVDVTPERLFTVGMLYLWTFCLVLTLLWAAGIGNVAERAKAQAALMEA
                     GKQRAEAAALRLQLNPHFLFNTLNSISSLMTLDRKAEAEEMMEKLADFLRASLEADPM
                     ADVPLARELDTIDAYLGIEAARFGERLEIDIAVPPAAADAMVPNFILQPLVENAIKYG
                     VATEKGRASLRISAETAAGELKLSVVNETLAGADGEASAGSPDAGAARPSTGIGLANI
                     RQRLEMGYGDSGRLETRALDDGFEATIHLPLKLAERRKAAE"
     gene            14832..15614
                     /gene="xth"
                     /locus_tag="VF589_13125"
     CDS             14832..15614
                     /gene="xth"
                     /locus_tag="VF589_13125"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8258567.1"
                     /translation="MRIATFNVNGVNGRLPRLIEWLEERAPDVACLQELKAPQDKFPA
                     AAIEKAGYGAIWCGQSRWNGVAILARGCEPIETRRCLPGDPDDAQARYIEAAVNGVLV
                     GCLYLPNGNPQPGPKFDYKMAWFERLNAYAAELIQADLPAVLAGDYNVVPTDVDIYNT
                     RSWLNNALLQPKPRAAYQRLLGQGWTDAIRHLHPDAPIYTFWDYFRNAWGRDAGLRID
                     HLLLSPAAAGRLKAAGVDREVRGREGASDHAPTWVELGDAEG"
     gene            15611..16117
                     /locus_tag="VF589_13130"
     CDS             15611..16117
                     /locus_tag="VF589_13130"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX8258568.1"
                     /translation="MTLKRSFAPVVDARTRLLMLGSLPGERSLERGQYYAHPQNQFWR
                     LMGAVIDADLAGLDYEARLAALLAAGVGLWDTVRSATRAGSLDAAIRDHSPNALRELV
                     ATLPELRAVGFNGGTSAAIGAKALAGETVELVPLPSSSPAYTLAFERKREQWLRLRAF
                     LDSPVVLA"
     gene            16147..16908
                     /locus_tag="VF589_13135"
     CDS             16147..16908
                     /locus_tag="VF589_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8258569.1"
                     /translation="MEIEVTSGAGVMDIRLDRPDKRNAISVAMYAALADAFEQAAADP
                     DILVVTIAGRGGLFTSGNDLRDFQQVREAGPDMPVFRFLRAIASCPKIVIAGVTGQAV
                     GVGTTLLLHCDLVVAATGATFSLPFVDLALVPEAGSSLLLPRLIGRQRAARHLILAEP
                     FDAETAFSYGLVTEIAAEGEVETRLRAMALSIAAKPPEAVRTTKRLLTSTDGSVAERI
                     AEEGALFGERLRSAEAAEAFAAFFEKRPPSFARPA"
     gene            complement(17076..>18510)
                     /locus_tag="VF589_13140"
     CDS             complement(17076..>18510)
                     /locus_tag="VF589_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8258570.1"
                     /translation="VFDYLKQAYLLSAKRALEVIDSAEGLDDSARTRARFFTEQYLNA
                     ISPANFAFTNPEAIRRAIDTGSISLLSGLANLLADAASEGGLVQRRSSEDFELGVTLA
                     ATPGSVVYQNELMQLIQYSPTTAEVYRRPVLYVPPLVNKYYLLDLQPKSSLIRWLVGE
                     GHTVFVISWVNPGSELAGKDIADYIRQGPIEALDVIQAATGERVVDLFGFCMGGTLIA
                     MALAWLAAKGEGDRVASATTIGSMFDFRTLGQWASFSEPEQLRAMERHLLHRGFLAAQ
                     DLQALFSSVRANDLIWSSVVNHYLMDREAPPSDILYWFADGSHLPRAFLLSWSQQLLR
                     DNKLREPGGVVLDGVPLDLGAIRTPLLAISLKDDHVSAWQATYDSARLFGGPVSFLLG
                     GSGHNAGVINPPSANKHGYWINDATPETAEDWFAGATKQEGSWWPHWQSWLSQGGTAE
                     KVPAREPGAGKLKAIEPAPGSYVRNRG"
BASE COUNT         3037 a         6284 c         6194 g         2995 t
ORIGIN      
        1 caattatccc ccgaatagaa gagataataa tagccggccc gccgaatcac ccaggccccc
       61 tcgatcaggc gctgatattc gtccggatcc tcggacttca ccggatggag gaggtcgacc
      121 ggctcgcttc cgggggcgaa cgagatccgg tcgggcgcaa gttcctgcac cttgatcggc
      181 tgaaagccgg atccccagta aagcagcctc ttgccggtcg ccggatcgtc atacgccatc
      241 ggatcgatat tgacgaagcc cggcccgcac agcagcggcc gcccgctgtc ggtgaaggga
      301 ccctcgggcg tggtcgccgt ggccacggcg aggcagaggc cgcggctcgg atcggccagc
      361 gccgcgtccg gcttggccga ataatagagg aagtagcggt cgccggcgcg gtggacatga
      421 ggggcccaga agtcctgcgt cttgctagcc caaacgggtt tcacaggcag cgcatcgccg
      481 agcagcttcc aatcgacgag gtcgtccgac cgcgcgacct gcaggttgat catcttgccg
      541 ccgcgctcgg tctgggtcgc ataggcgtaa taggagccgc ccggcgcctg gaggatcgca
      601 ggatcgggga agtcgccgtc gagaaccggg ttggcatagc tcgccggcgg cggctgccgg
      661 cccgcgggcg cgatgcagcc ggcgagcgcc gcgagggcgg cgagcatggt caaaatcggt
      721 gtgggtcctg tcacgggaag gcaggatagc gactccgccc agccatacaa tcgcgacgcg
      781 cgctcgatag ctcttctcat cgccgcccgg cactactatc gcgccgccat gaccagctcc
      841 gaaccgctcc tgtccttcgc cgtcgaggcc gccgccgagg gcgaagccac ccgcctctcg
      901 gtccacgcct ccgacggcac cgcccgcgac taccgcatcc ggggcggcga gcacccggat
      961 tatatcgatt tcttcgcgga gctcgcccgg gatttcggca cgcgcgtccc cggctccttc
     1021 gaggcgccgg ccgccgcgcc ttccgaggtc gcctggcggc cgctgatcac ccagaacctc
     1081 agcccgcgca ccctgtccgg ctacggcgac ccggcggtgc tgaaggtgga cggcggctat
     1141 atcctcactg ccaccagcaa cgatgcgccg gacgcgtttc caatcctctt ctcggccgat
     1201 ctccaacatt gggatcatcg cggcttcgtc ttccccgagg gcgcgactcc cgaatgggcg
     1261 gcggcgggct ggcacgtcgc cgatttctgg gcgccggaaa tggcccgcgt cggcgaggaa
     1321 tattggatcg cctacacggc gcgggaaagc tccaacgcgc tggcgatcgg cctcgccagg
     1381 gcgccgagcc ccgatggccc gtgggtcgac aatggcgcgc cgctgatcac cggcggcaag
     1441 gtgctttacc cggggacggc ggcgagcggc ggggtgatcg actcgcacat tttcgtcgat
     1501 catgatggca cgcccttttt gttctggaag aacgacacca acggactgtg gccgcgcccg
     1561 ctcgcggggc tgttgcgcga gcatccggcg ctgatcccga gcctgttcga cagcgaggcc
     1621 gaccggcgca gcgcggcctt cgcggcggcg gtccagccct gggcgaacac gcgccggccg
     1681 atggagcgct ttttcctgat gcagccgctg atcgaggccg cgctcgacaa ttgggccagg
     1741 gtaaagcccg ccctcgtcgg gttcggccat gccggcgaca tcgtcgaggc gatgcgcacg
     1801 ccgatccacg cccggcggct ggcgccggac ggccgctcgc tgatcgggga ggagaagatc
     1861 gtcctcgcca acgatctcga ttgggaaggc catctgatcg agggcccgtt cgtcacccgg
     1921 cagcagggcc gctattggat gttctacgcc ggcaacgact tctccacccc cgcttacggg
     1981 atcggggtgg ccgtcgccga ccacccgctc ggcccctacg tcaagcgcga ggagccgcta
     2041 ttgaagtcgc accgcttatg gtgggcgccg ggccatgcct cggtcgcgcc cggcctcgac
     2101 ggcaagccgc aattgttctt ccacgccttt ttcccgcacc agtgcgggta caaggcgttc
     2161 cgggcgctgc tgaccgtcgg gctcgagttc agcggggatg aggtgcggct gcgggcgatc
     2221 tgacgcggcg cggggaacag gtgcggcacc gctgccggct catcctagga gccgctgagc
     2281 gaagccgaag cagcgccaag gctcagtccc ccctcaggtt gagcggagag gatcgccgta
     2341 cctgtgcccc tgcgaaggca ggggcctaga cccggcgctt tggcggcagt ccggtcatcg
     2401 caccagggtg gaagcctggc gcggtagcac acacggtccg ggccccgcgc gatatccacg
     2461 agagcttggt tggcggactt cgcccggtct gggcccctgc cttcgcaggg gaacagaaag
     2521 gctggacccc tgccttcggc ccaagcttag cgtatccagc gggtttccgc cccgctagga
     2581 tcggcggcat gcaagtgtcc ccaccccttc tccgccgctg cctggctgcc ctgctcgcca
     2641 gcctgtcggc gatcgcgccg gccgcgtcgg aaccttcgtt cgtgccagtg ttcgagacca
     2701 atttcccgga cccccacgtc gtccagcatg gaggcgagta tatcgcctat gcgaccaatg
     2761 ccggcatcaa cctgccgatg gcgacgtccc gcgacctcgt caggtggagc ctggtcatgg
     2821 atccggcccg cccgaagcag cggctcgacg gcatgccgac gctcgcgccg tgggtgaagg
     2881 aaggtttcac ctgggcgccc gaggtgatga aggtcggcga gcggtggctg ctctattaca
     2941 cggccaacca ccgcaagcgc gacgtccagt gcatcgggct tgccgtggcc gacaatccga
     3001 aggggccgtt ccgcgatgcg tcggcaaatc ctctgatctg ccagttcgag ctgggcggca
     3061 cgatcgacgc caacccgttc cgagatgccg acggcaagct ctatctatat tataagagcg
     3121 acggaaaccg cgtcggcaag ggtacggcga tctgggggca gcggctcgcg cccgacggcc
     3181 tgtcggtggt cggcgcgcct gccccgctgg tgaaggacga caagccttgg gagatgaagc
     3241 tggtcgaggc gcccgccatg gtgcgctcgc ccaccggcta ccagctgttc ttcgccgccg
     3301 cttattatgg ctggaacgcc gaggaccggc tgtcccctta cgcgaccggc tatgcgacct
     3361 gttcgggccc gctcggtccc tgcgtcgacg cacccgaaaa cccgatcctg cacagcttcc
     3421 gggagcggga ggcgggctgc ctaagcgggc cgggtcatcc cagcatcttc gaggcgcggg
     3481 gccgcagctt catcgccttc cacgcctggg ccgcgaccaa gggctgccgc aagtccgagg
     3541 actcgcgcta cctctatatc gccccgatct tttgggagca gggcaagccg cggatcgcgc
     3601 ccagccttcg tccggccgcg aaactcgggg agcgccgatg aggctggcgg ccttggcgct
     3661 atgcctcggc actgcctgtc cggcggcggc gcaggagacg gacgatctct accgctaccg
     3721 cgccggtgag gagacgcgct gggtcagccc cgaaaacccg accggtgcga agggcgccgg
     3781 cgccgccgag aatcgcggcg ccaagggcca tgcgttcgag acgatcgcca tcgggcgctc
     3841 ccacgtactg gcggacataa agggcgccgg catcatcgac cggatgtgga tgacgatcga
     3901 agaccgctcg cccgacgcgc tgcgcggcct caagctcgaa atcttctggg acagggcggc
     3961 gacgcccgcg gtgtcggccc cgctcggcga cttcttcctc cacggcgccg gcgagatggt
     4021 ggcgatggag acggcgctgc ttacgagccc cgagggccgc tcgttcgtct cctacattcc
     4081 gatgccgttc cgtaagcgtg cacggatcgt tgtcaccaac gaatcggcca agccggtcaa
     4141 cctcatcttc tacgacgtca actatcgcgc cttgcgccgc cagccggacg atgcgctcta
     4201 cttccacgcc tggtggagcc gcgaccgggc gaccaccctc ggccgcgatt tccgcatcct
     4261 gccgcggatc gcggggcgcg ggcgcttcct aggcgcgagc gttaccgtcc tcaccaaccc
     4321 cgtctacgag aagacctggt ggggcgaagg cgaggtcaag atcgcactcg acggcgaccg
     4381 ctccggccgc ccgactttgg tcggcaccgg cgccgaggat tatatcggca ccgcctgggg
     4441 ccagggcgcc tatatcaacc gcttccaggg cgcgcccatc gccagctggg acaatgacgg
     4501 ccgctggacc ttctatcgct tccacgtgcc tgacccgatt tggttcagac acgacatcga
     4561 ggtcagcctg cagcagatcg ggggagcgag gaaggcggtc gtgctcggcc ttcagaagaa
     4621 gggcgcgccg ctgatcccgg ttacgatcga cccgggctca cgcacgaact tccagcaatt
     4681 actgaccagg gacccgccgg tgccgctgtc ggacccgtcg ctgccggacg ggcataccaa
     4741 tttctaccgc agcgacgacg tcgcggccgt cgcctatttc tatctcgacc ggccggagcg
     4801 ggcgctgccg ccgatcgccg cgccggccga gcgccgggca gggttgcgga tggcgccgcc
     4861 gcctcaggcg aagtagacgc cgcactcgtt gccctgcgaa ggcaggggcc cagaccaggc
     4921 gaagcctgtc aaacgagggt agaggtcgcg ccggtccggt tcatcggtgc cggtgaggcc
     4981 gctcgccatg atgtaaacag atccggcttt cgcccgagag catggccgga ctgccgctaa
     5041 agcgccagcc cgggactggg cccctgcctt cgcaggggaa ctgctgaggc cggcacggcc
     5101 gggagtccgg cccctgcccg ttgcgaaccc atgtgggatt cgcaacgagc tccattccag
     5161 ccgcagtttg cgatcagagc cggaaacggg cgccgagcga gaaggtctgc tcgtcgtagc
     5221 gcaggaagcg gacgaagtcc gcgcgcggcg cacccgcccg tgccgagctg aagtcgaccc
     5281 ggaaggcgtc gcccgtgagg ttggtcgcat cgaagaatac ggtgaaattg tcgttgatgt
     5341 gatagctggt cgaaaaatcg agccggcccg caggccgtgc gatctcgcga tagaaaccac
     5401 cctcttcgtc gccccgcaca tcgcgccgat caaggtaacg gctccgcttg ttgtaggtaa
     5461 gccgggccga gaacggaccg ccttcgtaca aggcggcgat attgaaggtg tgcttggaaa
     5521 cgccgcccgt gtcggggtcg ccggacgagg tgatctgatc cgagatgaac tcgcccgtca
     5581 gcggattgaa tatttctacc ttgccgtcga gataggtgta gttcgcctgg acgccgaagt
     5641 tgcggaggaa gtcggggacg cctccaaact cgaagaaggt cgtcacctgg gcttcgagcc
     5701 cgtcgatgcg cccgctacgc gtgttcaccg gcaccgtgat acgcaccggg ccgagcgctg
     5761 gatcgttgaa gtccggcagc gtctggtcct ggatgaatcc cttcagatcc cgccggaaaa
     5821 cggccagcga agcgaagccg gtgcgcgaga agaaatattc cagcgacaga tcgtaattgt
     5881 tggattcgat cggccgcaaa ttgggattgc cgccgttgcc gatccgcaag ccgccctgca
     5941 gcgggtccgg tgcacccaac gtgaccgccg ggttcagctg ggcgaagctg ggccgcgtgc
     6001 gcgtctgcgt gtaggacaag cgagcctgca gctgcggggt gatccgccag cgcaggttgg
     6061 ccgtcggcag gaaatcggta tatttctgcc ccgtctcacc cccgatgctt tcattgatat
     6121 cttcgaactc gctttttgtc cccaccacgc gcaggccgat gacgccgtcg agcgactcgc
     6181 cgaactggta gttgatctgg ccgtagcccc ccagcgtctt ctcgttcgcc gtgtacagcc
     6241 gaacccgctc cacgtcctcg agcgtgtagt tggtcgcgcc gcggtcgatc acgaactggc
     6301 gcagtgcgac gatattctcg cggatgctgt cgtaagtcgg cgtcacgaac gtctcgaacc
     6361 ccgactgcac gtcggtgccg cggaagcccc ggcgggccag ttcgaactcc agcggcaatg
     6421 cggtacccgg gatatcctgg ccgcggaacc cggcgaaacg attgccgaac tcgcggatcg
     6481 cgtcgcggtt ggtgtggcgg acgccggcct gcaagctgcg gatgaaaaag tcggactgga
     6541 actcatattc ggcgtcggcc cgcaactggt aatcgtcgcc cttcgacacc tgattctctt
     6601 cgtacaggcc gtcgaacaag aagttgtcgg gattggtcgc gtcgaacccc ctgatggtga
     6661 attgcggcgt ggtgagatcg aaatcgacgg tcgtattctc gcggccggcg aagatgcgat
     6721 ccaccgactc ggtcgacccg gtaaacttgc tgctcgtgcg ggcggcgtcg gcggtgagct
     6781 tgaacgggcc cgattcgtag atgccgccga tggcgaactg atacgtgtcc gtcttgttga
     6841 aggtgccgcc ctggaaggtg aagataggat cgccgaggcc ggtgaccgtt ccgctctcaa
     6901 gcaggttggt gccctcgcgg aagacgagat tggtgtagct gccgccgcca tagagcggca
     6961 ccgccaccag ccgatcggaa atccggttgc ggaacccctg gtacaagcca tcgatataga
     7021 attcgagcgt gtcgttgggc cgccattgaa gggcgacgtt ggccgacggc cggctgcgat
     7081 cgccctcgcg gaagaacagg cgctggatgt cggggaagcg gacgcgttcg ccgttgatct
     7141 cgggatcggc gacgaaatcc gtgttcgacg gctccgaatc caggaacttg agctgcgtat
     7201 aggaggcatt cacgagaaac ccgatctcgc cgatgccggt gtcccaacga ttgctgatca
     7261 ggatgttgcc gttgggcgtg atctcgcccg cctgcgccgt ataaagcccc cagaccgagc
     7321 cggcgacttc cagacccttg aagtcgaacg ggcggcgtga acggacgttg accagaccgg
     7381 cgaggcccgc ttccaccagg ctcggcgtgg tggtcttgaa gacctccagc gcggcgatgt
     7441 ttgccgacgg gaaatcctgg agcgcgacga cgcgggtctc ggccgtgaag atctcgcggc
     7501 cgttgtaggt ggtggcgaaa tccggaaggc cgcggatgag caccgtcgaa gcctcgccgc
     7561 cggtccggat tacctggacg ccggggatgc gggccgccgt gtcggagacg gcaaggtccg
     7621 ggagcttacc gatatcctcg gcgacgatcg aatcgacgat ctgctcggaa ttgcgcttga
     7681 tgttctgcgc cgactgaagg ctgcgccgga ggccggtgac gacgatctcc tcttccccgg
     7741 caacagtctg gtcggcgccg tcgacggtct ggaccgcatc gtcggtctgc ggcgcgccct
     7801 gctccggatc gccgggcgat tcctgcgcct ggacggtggg atcgaccggc gcggcgggat
     7861 cgacctgggc cgcggcggcg gtggatgcca gcgccagcac cgcgagcgat gaagtcgtcg
     7921 agaagacacg atggaagcgc atgttcaggt tcccccttgg ccatacccta atggcccgcc
     7981 cccgggcctg aagcgggcag gtatatccac gccggatcga tcctgacaac atacgagact
     8041 cattaatgat tcactgtggc atatgagacg cagcggcggc cgcgcccctg aggccggaaa
     8101 gcaacagggc ggttgcggtg gtcggttgtt cgtctaaagt cgacggcgag agggagcatc
     8161 attggccagc ctggaactga tcgacatcgc caaggcctat ggcagcacgg ccgtattgga
     8221 cggcgtctcc ctcaccatgg aaccgcggga gttcgtcgct ttcctggggc cgtcgggcag
     8281 cggcaagtcc acgctcctgc gcatcatcgc cggcctggag acgctcgatg ccggcgaagt
     8341 gcggctggac gggcgacgga tcgacggcct gccgccgggc gaccgcggcg tcgccatggt
     8401 gttccagcat tacgcgctct atccgcatat gagcgtacgc gacaacatgg ccttcgggct
     8461 tcgcaacgca aaggtgccga cgcccgagat cgaggcgcgg gtggcggagg cggcgcgcat
     8521 cctcgagatc ggggagcatc tcgacaagaa gcccggccag atgtccggcg ggcagcggca
     8581 gcgggtcgcg atcggacggg cgatcgtcaa gcatcccaag ctattcctgc tcgatgagcc
     8641 gctgtcgaac ctcgacgcgg cgctgcgcat gcgcacccgc gtcgagctcg cccagctgcg
     8701 gcagcgggtc gacgcggccc tgatcatggt gacccacgac caggccgagg cgatgacgct
     8761 ggccgaccgg atcgtggtga tgaacgaccg ccgcatccag caggtcgggc cgcccatgga
     8821 gatttacagc cggccggcca acaccttcgt ggcgcgtttc gtgggatcgc cggcgatgac
     8881 gctggcgccg gtcgatctgc tgcccggcga cggaccctat gcgaccgtgc ggatggggaa
     8941 cggcgccgaa attgccaccc gggtgccccg ctccggcctg cctgcgggcg gcgcattgca
     9001 gctcgggctg cggccggaga gcgtgaaagt ggccgccaac ggggccattg cggcggaggc
     9061 gaaagtcgac ctcgtcgagc ggctcggcga ccgtaccctg gtctatgcga cgctggcgga
     9121 cgggcttgcc gtcaccgccg aggatgaggg caacagccgg gtccggctcg gcgacacgat
     9181 cgggctcgcc atcgacgggg cggcggcgca tctgttcgac gccgagggcc gcggccacca
     9241 tgcggaggcg cggcccgcat gagcgcgagc ggcggccgca gggactatag cggcctgctc
     9301 ttcgtcctgc cgttcctcac cgcctatctg gtcatcctca tctacccgtt gctcagcggc
     9361 atggcgctca gcctccagcg cgtcgacctg ttcgggggcg gccgcttcgt cggcttcgac
     9421 aattatgccc gcctgttcgc cgacgacagc ttcgtccgct ccatcctcaa cactttcgcg
     9481 ctggcgctga tgatcgtgcc gctgctgaca atgctcgcgc tggggctggc gctcgcgctc
     9541 aaccgcgcga cccgcggcgc cgcggtcttc cgcggcatct tcttctcctc ctcggtgctg
     9601 tcggtgacca tcgtcaccct catctggcgc ttcgtgctgg cgccggacgc cgggctgctg
     9661 ggcgagacgg cgcaggccat cggcgccgag ccgatcccgt tcctcagcca cccgacctgg
     9721 tcgttatggg cgctcgccat caccaccatt tggtggtcga tcgggttgcc gatgatgctg
     9781 ttcctcgccg gcctccagca gatccccgcc gacatatacg aggcggccgc gctcgaccgc
     9841 gccagccgct ggaccaccct caggcgcgtc accctgccct cgctgcggcg caccgtcgtc
     9901 ctcgtcgtca tgctgcagac ggcggcccag cttcagatct tcggccaggc ccagctcctg
     9961 accggcggcg gccccggcgg cgccacccgc accatggtgc tgttcatctt cgaatccgcg
    10021 ttcgcgcgct gggagcttgg ctatgccacc gccgcggccg agatattgtt cctgctgatc
    10081 gtgctgctca cgctcaccca atatgtgacg accatgcgcg gatcggaggg cgacggtggc
    10141 cgctgaagca agccgcaccc gctggtctcc gctgacgttg cttgcgatcc tgctcgcggc
    10201 cttcgtgatg ctcgcgcccc tcgtctggac tttgctgctc tccttgaagg agaatgaggc
    10261 tctgatcggc aatagcggcg ccgctttgtc gccgccctac accttcgaaa actaccgctc
    10321 gatcctgcag ggaaaccagg tcttccgctg gctggtcaac agcatcatcg tctcgggcgg
    10381 gaccacgctc ggcgtgctgg ctctgtgcag cctcgccggc tacggcttcg ccaggctcga
    10441 tttcccgttc cgccgcacgc tcttcgtctt cgtgctgctg ggcctcgcca ttcccgagca
    10501 ggcggtgatc ctgccgcagc atcaattgtt cgccgatctg cgcctccaca attcctatcc
    10561 cggcctgatc ctgcccggcc ttgccggccc gttcggggtg ttcttcatga cccaatatat
    10621 gcgcgccatc cccaaggagc tcgacgaagc ggcgatgctc gatggcgcct cccgcttcac
    10681 cttgttctgg aaggtgctgc tgccgctcac cctgccggcc caggcgacgc tcggcgtctt
    10741 caccttcctc gcctcgtgga acgcttattg gtggccgctc atctcggcga cgcgcagcga
    10801 catgtacacg ctcaccgtcg ggctcgccgc ggcccagatg aactatgccc agaccagcgg
    10861 cctcggcttc ctgatggcgc aggccgtgtt cgcctcgatc ccgatcttca tcgtctattt
    10921 gctgttccag aagcagatcg tgcgcgcgat ggcggggacg gcggtccgtt gaggcgcctg
    10981 ttcatcctgg cggcggcttg tctcgccgga agctgcagcg gcgacgccgg cggcggcgat
    11041 cgcgccgaaa tctacctcca gcgcttcttc ggcgagtgcg gcgccgaata tggcggcgcg
    11101 accgacgtct cgaaggtcga gggcgagtgc ggcatcgtca ccgcgatgat caacaagttc
    11161 aacgcccgca accccgacgt ccacgtcagc agcaacgtcg tcgcctggcc cggctatccg
    11221 cagctttccg cccagatggc ggcgcgggac ccgcccgacc tcgtcaccat gcataccggc
    11281 gtgatctcgg actattctgc cgaaggcctg ctcgagccgg tcgagcccta cctggccgag
    11341 gccggcctct cgccctccgc cctcaccgaa gcgagccgca gcgccgtgac catcggcggg
    11401 cggctctacg ggctgccgtt cgacacccat ggcggcctgt tccacatcaa caccaaattg
    11461 ttcgccgagg ccgggctcat caaggacggc aagcccgtcc tccccacgtc gccccaagag
    11521 atgctggccc acgcccgcca attcaccgaa cggaccggca agccctatct gatccagagc
    11581 ctggtcggcg acaatgccta tgccgcccgc aacgtctata cctatctgct ggcgcagaat
    11641 gcggtgctgt tccccactcc gcgctcgatc cgcctgcaga cgccggaggc cgagcggatc
    11701 gtcgacttct tccgccagat caccgcgtcg ggggtgagca cgcgcaatca ggacacgccg
    11761 gccgcgatcg ccagcttcat gtccggcgag ggcggcatct atcccaccgg cacctggatg
    11821 atcggccagt tcgaggccga agccaacaag ccggggcggc cgctctaccg cagctacacc
    11881 gtctatcctt atccgcggct atggggcgag aacgtcaatt atgtgagcgg ccacgcttgg
    11941 gtggtcccaa aacgcgagcg cacgcccgag cagcgccgcg ccattgcccg cttcttccgc
    12001 ttcatcgcgg agcataattt cgactgggcc cgcaccggcc acctccccgc tttcaccgca
    12061 gtggtggaca gccccgcctt cagggcgctg ccgcatcgcg ccaacatcgc gccgatcgcc
    12121 cgcaacggcc gcccgctccc cggcgaggtc cagcgccaga atgccatcga aggcctggtc
    12181 ggcgaggaag tcgcggctgc cttcaacggc cagaagtcga cccgccaggc gctcgccgac
    12241 gccgaacgcc gcgtcaacgc cgtgctggcg gggatcgcct agcgggcgga aggcccgcgg
    12301 cgctccgtct catcggctgg agctttccat aggtcgccga ccgccatagc cattcgaacg
    12361 ggccgaagcg gaagcggcgg atccaggccg cgctgaacag gcacaaggcc gcgaacagga
    12421 tcagggccag caggttcatc gccgcgaaat tccaggcgcc gagctgcccg aggccccagc
    12481 cataccagat gaacgctccg gcggcgttgg aggcgaggta gccggtcagc gccatccggc
    12541 ccatcggcga cagcgcccgc tggacgccgg ccccgcccct ggaaagccac agggtaagta
    12601 tcgcgatata gccgaaggcg gcgatcggga ggcccgcata agcggcggtg acgaggccgt
    12661 aagcggcgcc gtccgcctcg atccccatcc aacgggtagc gtggatgagg ctgagcaata
    12721 gcccgagcgg caggcacagc caggccagct tgcggaacag cggccgccac cgctccacat
    12781 cggcgaacag cccgccccgc gccgcccaca gcccgagcag gaacaggccg agcacgccga
    12841 catcgtaggt gaccgcatga gcggtgtcgg tccgataacc gtcgaaatag agctgcaaat
    12901 tggcctgcac gaactcggca tagctgccgc ggctatagac cggctccagc gcggccatgg
    12961 cctcgtcggc ggcgtccggc cccagccccg caaggctcgg catcggcgcg ccggtggccg
    13021 cttcgatggc tccgaagacc actggcggca gcaggatcaa ggccaggccg aggctcagga
    13081 ccagacggtc gctggcgttg cggaaggcca gcatggctat gccgagcagc gcgtagagga
    13141 tgaggatgtc gccccagaac aggaaggcga ggttacaaag gccgatcgcg agcaaagcgg
    13201 tcatgcgggc aaggtagaag cccacgaagt tgctgccctt cgtggcggcg cgcgccatca
    13261 ggatgccgaa accgacgccg aacaggaagg cgaagcagga gcgcgccttg ccctggagca
    13321 cgatcagatc gaagctggcg aaggcgaggt cgagcgaggt ggcggcgggg aatatccggt
    13381 cgtccgccag cgccgccacc atgccggtga tgttcatcgt tatgacgccg aacagagcga
    13441 tggcgcgcag cgtgtcgatc aggtgaagcc gctcttcggc cggtgccatc agggtcggtt
    13501 ccgggacagt gcgggtttcg ctcatggcat tcccctttgc tcgggcggcc cgctgcaagg
    13561 ccggaacccg atcctcgaac cgccttactc cgccgccttg cgccgctccg ccagcttcag
    13621 cgggaggtgg atggtggctt cgaagccgtc gtccagcgcc cgcgtctcca gccgcccgct
    13681 gtcgccatag cccatttcga ggcgttggcg gatgttggcg aggccgatgc cggtgctcgg
    13741 cctggccgcg cccgcatcgg gcgagcctgc cgatgcttcg ccgtcggccc ccgccaaggt
    13801 ctcgttgaca acgctcagct tgagctcgcc cgccgccgtc tccgccgata tgcggagcga
    13861 ggcgcggccc ttctccgtcg ccacgccata tttgatcgca ttctcgacca gaggctggag
    13921 gatgaaattg ggcaccatcg cgtcggccgc ggcgggcggg accgcgatgt cgatctcgag
    13981 ccgctcgccg aaccgggcgg cctcgatgcc gagataggcg tcgatcgtgt cgagctcgcg
    14041 cgccagcggc acgtccgcca tcggatcggc ctcgagcgag gcgcgcagga aatcggccag
    14101 cttctccatc atctcctcgg cctcggcctt gcggtcgagc gtcatcaggc tggagatgct
    14161 gttcagcgtg ttgaacagga aatgcgggtt gagctggagc cgcagcgccg ccgcctcggc
    14221 gcgctgcttg ccggcctcca tcagcgccgc ctgcgccttg gcgcgctcgg cgacattgcc
    14281 gatgccggcg gcccaaagca gggtcagcac caggcaaaag gtccagaggt agagcatgcc
    14341 gacggtgaac aggcgctccg gcgtgacatc aaccgcccag cgctgccaat ccggcacgaa
    14401 ggtgagcgac agccatttga aataataaag gtcggcgaat gagaagatgg cggtcgccgc
    14461 cagcaccgcc acgccgagac cggcccaggc gatcagccgc gggcgcaatc gcagcctttg
    14521 ccgcagcccc tcgagcagca tcgtgaaagt gatgccgagc acgaataacg ggatcgacac
    14581 caatgtactg acgcccggcc ccatccgccc catagccgtc accgtgatgt cggcgagcag
    14641 ataggcgaag gcccagatga cgagcaccgg gcgcagcgcc cgatccgcaa gcattcctct
    14701 cagccggttc atgcgccctc ccggccgcac catagcggtt cccgaacggg gggccagcat
    14761 ctgtttgagg ccggcgtgcg ggcgcgcgaa tcctgctgca ttcggtcggc ggacgcgcta
    14821 gcggcgggtg ggtgaggatc gcgaccttca acgtcaacgg cgtcaacggc cggctgccgc
    14881 ggctgatcga gtggctggag gagcgggcgc ccgacgtcgc ctgcctccag gagctgaagg
    14941 cgccgcagga caagtttccg gccgcggcga tcgagaaagc cggctatggc gcgatctggt
    15001 gcggccagag ccgctggaac ggcgtcgcca tcctggcgcg cggctgcgag ccgatcgaga
    15061 cgcggcgctg cctgcccggc gaccccgacg acgcgcaggc gcgctatatc gaggcggcgg
    15121 tgaatggcgt cctggtcggc tgcctctatt tgcccaacgg caacccgcag ccggggccga
    15181 aattcgacta taagatggca tggttcgaac ggctgaacgc ttacgccgcc gagctcatcc
    15241 aggcggacct gccggccgtg ctcgccggcg actataacgt cgtcccgacc gacgtcgaca
    15301 tctacaacac gcgctcctgg ctcaacaacg ccttgctcca gccgaagccg cgcgcggctt
    15361 accaaaggct gctcggccag ggctggaccg acgccatccg ccacctccac ccggatgccc
    15421 ccatctacac tttctgggac tatttccgga acgcctgggg ccgcgatgcc gggcttcgca
    15481 tcgaccatct gctgctgagc ccggcggcgg cgggccggct gaaggcggcc ggcgtcgacc
    15541 gcgaggtgcg cggccgcgag ggcgcgagcg accatgcacc gacttgggtg gaactggggg
    15601 acgcagaggg gtgacgctga agcggtcgtt cgcgccggtc gtcgacgcgc ggacgcggct
    15661 gctaatgctc ggcagcctcc cgggcgagcg atcgctggag cggggccaat attacgccca
    15721 tccgcagaac caattctggc ggctgatggg cgcggtgatc gacgccgacc tcgcggggct
    15781 cgattatgag gcgcggctgg cggcgctgct cgcggccggg gtcgggctat gggacacggt
    15841 ccgctccgcc acccgcgccg gcagcctcga cgccgcgatc cgcgaccaca gccccaacgc
    15901 gcttcgggag ctggtcgcga ctttgcccga gcttcgcgcg gtcggcttca acggcggcac
    15961 ttcggccgcg atcggcgcca aggccctggc gggagaaacg gtggagttgg tccccctccc
    16021 ctccagcagc cccgcttaca cgctcgcctt cgagcgcaag cgggagcaat ggctgcggct
    16081 gcgcgccttc ctcgacagcc ccgtcgtgct cgcttaagcg ggcggcggcc gcgggagggc
    16141 agtaagatgg agatagaggt tacatccggc gcgggcgtga tggacatacg cctcgaccgg
    16201 cccgacaaaa ggaacgcgat cagcgtcgcc atgtatgcgg cgctcgccga cgcgttcgag
    16261 caagcggcgg ccgatcccga catattggtg gtgacgatcg ccgggcgcgg cgggctgttc
    16321 acttcgggca acgatctgcg cgatttccag caggtgcgcg aggccgggcc cgacatgccg
    16381 gtattccgct tcctgcgcgc catcgcttcg tgccccaaga tcgtgatcgc gggcgtcacc
    16441 ggccaggcgg tcggcgtggg cacgactttg ctgctccatt gcgacctcgt cgtcgctgcc
    16501 accggcgcca ccttctcgct gccgttcgtc gacctggctt tggtgcctga ggcgggaagc
    16561 tcgctgctgc tgccgcggct gatcggccgg cagcgcgcgg cccggcacct cattctggcc
    16621 gagccgttcg acgccgagac cgccttttcc tacggcctcg tcaccgagat cgctgccgaa
    16681 ggcgaggtcg agacgcggct gcgcgcgatg gcgctcagca tcgccgccaa gccgcccgag
    16741 gcggtacgga ccaccaaaag gctgctgacc tcgaccgacg gatcggtggc cgaacgcatc
    16801 gccgaggaag gcgccttgtt cggcgaacgc ctgcgctcgg ccgaagcggc cgaggcgttc
    16861 gccgctttct tcgagaaacg cccacccagt ttcgcgcggc ccgcttagcg ctcgatcccg
    16921 ctcaggggaa aggtggggga aaaaggcggg ggctgctccc taatccgttc gtcctgagct
    16981 tgtcgaagga ctttccttct ttcaggcaga agggaagtag agggcttcga caagctcagc
    17041 ccgaacgggt tgggggcgcg ccgcccggcc gctatctaac cgcggttgcg gacgtagctg
    17101 cctggggcgg gctcgatcgc cttgagcttg ccggcgcccg gctcgcgggc gggcaccttc
    17161 tcggccgtgc cgccctgcga cagccagctt tgccagtgcg gccaccacga gccttcctgc
    17221 ttggtcgcgc cggcgaacca gtcctccgcc gtctccgggg tcgcgtcgtt gatccaatag
    17281 ccgtgcttgt tggccgacgg cgggttgatc accccggcat tgtggcccga gccgccgagc
    17341 aggaagctga ccgggccgcc gaacaggcgc gcgctgtcgt aagtcgcctg ccaggcggag
    17401 acgtggtcgt ccttcaagga gatggcgagc agcggcgtcc ggatggcgcc gagatcgagc
    17461 ggcacgccgt ccagcacgac gccgccgggc tcgcgcagct tgttgtcgcg cagcagctgc
    17521 tgcgaccagc tcaagagaaa cgcccgcggc aaatgcgagc cgtcggcgaa ccagtaaaga
    17581 atgtcggacg gcggcgcctc gcggtccatc agataatggt tcacgaccga cgaccagatg
    17641 aggtcgttgg cgcgcaccga gctgaacaaa gcctgcaggt cctgcgcggc gaggaagccg
    17701 cggtggagga ggtgccgctc catcgcgcgc agctgctcgg gctccgagaa gctcgcccac
    17761 tggccgagcg tgcggaagtc gaacatcgat ccgatcgtgg tggcggaggc gacgcggtcg
    17821 ccctccccct tggcggcgag ccatgccagt gccatcgcga tgagggtgcc gcccatgcag
    17881 aagccgaaca ggtcgacgac gcgctcacca gtcgccgctt gaatcacgtc cagcgcctcg
    17941 atcggcccct ggcggatata atcggcaatg tccttgccgg cgagctcgga gcccggattg
    18001 acccaggaga tgacgaacac ggtgtggccc tcgccgacca gccagcggat caggctcgac
    18061 ttgggctgga ggtcgagcag ataatatttg ttcaccagcg gcggcacgta gaggaccggc
    18121 cgccggtaga cctcggccgt cgtcggcgaa tactggatga gctgcatcag ctcgttctgg
    18181 tagacgacgc tgcccggagt ggcggcgagc gtgacgccga gctcgaaatc ctcgctggag
    18241 cggcgctgca ccagcccgcc ttcggaggcc gcgtcggcga gcagattggc gagcccggag
    18301 agcaggctga tcgagccggt atcgatggcg cggcggatcg cctcgggatt ggtaaaggcg
    18361 aaattggccg gcgagatggc gttcagatat tgctcggtga agaagcgggc gcgggtgcgg
    18421 gcggagtcgt cgaggccctc ggcgctgtcg atcacctcga gcgcgcgctt ggcggacaac
    18481 agataggcct gcttcagata atcgaacacc
//
LOCUS       DASVRK010000316        30613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_60885, whole genome shotgun sequence.
ACCESSION   DASVRK010000316 DASVRK010000000
VERSION     DASVRK010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 30613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30613
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60885"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VF589_13145"
     CDS             <1..317
                     /locus_tag="VF589_13145"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX8258571.1"
                     /translation="QIEAAVRLRAPVVELHTGRYAHLEGEARADELRRLAGAAALAAK
                     NGIEAHAGHGLSFANVVPVAAIPQLAELNIGHFLVGEAIFTGFEASVRRMRDLMDEAR
                     GA"
     gene            459..890
                     /gene="acpS"
                     /locus_tag="VF589_13150"
     CDS             459..890
                     /gene="acpS"
                     /locus_tag="VF589_13150"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX8258572.1"
                     /translation="MIIGIGSDLCNIERIQASLDRFGERFIARVFTETERAKAERRPF
                     TRAGTYAKRFAAKEAYSKAVGTGFRRGVFMKDIGVVNKPSGEPTLHLTGGAKQRLDDM
                     TPEGHAAEVHLTMTDDHPFALAFVIITAVPLRDPATQGARG"
     gene            914..1765
                     /gene="lepB"
                     /locus_tag="VF589_13155"
     CDS             914..1765
                     /gene="lepB"
                     /locus_tag="VF589_13155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8258573.1"
                     /translation="MTEKSVKTKPKGQTDWWSEIKGIFWLILAVLAFHSFVAKPFYIP
                     SESMMPVLLKGDRLVVSKYPYGWSWVSPSFHVLPQMGGRLFGSLPERGDIVIATPPGQ
                     SADYIKRVVGLPGDELEMIDGQLIINGLPVERAEQPARMVPVDDNAPCLPTEFPDARV
                     KGRDGNAYCRLPVVRETLPNGRSYDTIDLGYSTTDDFPKIKVPDYHVFLMGDNRDRSA
                     DSRVALDQQGLGGPIPYENIGGRAEFITFSLDGTSQYLNPISWFTALRGDRAGSTLHP
                     TQGAPTE"
     gene            1822..2835
                     /locus_tag="VF589_13160"
     CDS             1822..2835
                     /locus_tag="VF589_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8258574.1"
                     /translation="MIRQELKRASVWFGIGVGLLALFFLAQPLLLIFAGIVLAAMLDG
                     GTRLLGRVLPIGRGWRLAIVTILAIGFVVWTFVFAGQTIAAQAESLRTAVTFQLNRIL
                     AWGNELGIVQGGAGIQQLSGQLMGSLGRLGSAVSSALGALTSMVMILVIGIFIAVEPR
                     LYERGFAWMLPIRGRDEFYRTASHMGRTLRRLMAGRLIGMAVEGFGTWLLLWIGGVPM
                     AALLGILTGLLAFIPNVGAIVSGVLIVLAGFSVSTDAGIWAIGVYLIVQTVDGYLIVP
                     YVARKTVDLAPALVLGAQLLFGALFGIMGLALADPIVAMIKAALEQKSREESGLAAGV
                     AEP"
     gene            complement(2840..3394)
                     /locus_tag="VF589_13165"
     CDS             complement(2840..3394)
                     /locus_tag="VF589_13165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8258575.1"
                     /translation="MSVTAVPIRPVKKGTVVKLWVGLGALALGAAGIAWAGTSTQVAM
                     GDPAGFLASNGKREGVTTTASGVQIQTIRPGSGARIGANDGVIIEYKGRLLDGTVFDS
                     TEGKGPAPMLVGQVVPGFSEALQLMQTGGAYRIWLPPQLAYGEAGTPGGPIPPNAVLD
                     FDVSILQVVPNAAAQAAGQLPPGL"
     gene            complement(3602..3808)
                     /gene="rpsU"
                     /locus_tag="VF589_13170"
     CDS             complement(3602..3808)
                     /gene="rpsU"
                     /locus_tag="VF589_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX8258576.1"
                     /translation="MQIIVRDNNVDQALRALKKKLQREGVYREMKLRRHYEKPSEKRA
                     RERAAAVRRARKLERKRMERDGVR"
     gene            complement(4777..6993)
                     /locus_tag="VF589_13175"
     CDS             complement(4777..6993)
                     /locus_tag="VF589_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258577.1"
                     /translation="MAFINGTGGTDMLNGTSSDDYIAGRGGTDFLDGGHGADRLYGGD
                     GHDVLYDLADGDAGDALFGEAGDDSFQVYDTEADTLDGGVGSDWYYVNGDMATVVDLV
                     NQSANAGHAAGDRLRSIEQFRGGSGNDRFLGSNQNEGFQDADGDDFYNGRGGTDIYYA
                     AMPIGPDDVDFEIAYGNRAIDLAAIKGITLSSPQSGVAFYEMWTDGNADSIRDLSEVT
                     YQIDELVGVEHYLSPGMGAIMAGSAANEVFYCSNMPIRVDGGGGTNDFVAFGGIGFAG
                     AMIIDLAAGFASDGAVMATLMRIESAMGSDSSDQITGNGLVNTLVGLTGDDLLDGRGA
                     NDRLDGGAGMDLLVGGDGNDVLMGGALGDTLDGGLGIDTACYADASNNLSITLATGAK
                     GMDQGDGDTFVSIENVIGSRNGDAVHGNGVANRIEGREGGDRLMGGGGDDRLFGDFDP
                     GSAVAPGVTSFDPTVTIGDQAEIEDESEYAESNYLYGQGGNDILVGGDEYDDLYGGAG
                     NDQLDGGGGSNNLYGGAGNDIYIVGGSDDRVSERAGEGSDRVIASENHYLYDNLESLV
                     LTGTAAIGSGNGLANSILGNTAANRLYGGDGEDRLNGGGGDDRLSGGDDADSLTGGTG
                     RDRFRFDSGIDGTDNIDAIEDFVAADDTILLDRAIFDAIAADGRLAASAFRAGTEATD
                     ASDRIVYHQATGRIFYDADGSGGSAVAVLFATVDVGTILTSADFVAVSAPNLGQDG"
     gene            complement(7012..7137)
                     /locus_tag="VF589_13180"
     CDS             complement(7012..7137)
                     /locus_tag="VF589_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258578.1"
                     /translation="MAIVKSSLSLIRYPRLSAAADRGGADARAFAAEGADRPADI"
     gene            7312..7881
                     /locus_tag="VF589_13185"
     CDS             7312..7881
                     /locus_tag="VF589_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8258579.1"
                     /translation="MSRFTVNGQPIHYRLDSDTPLLWALRDASNLTGTKYGCGTGHCG
                     ACTVNIDGNAVRSCLVRIGNLEGSFVTTIEALSRDRSHPVQQAWVADQVPQCGYCQSG
                     MIMAAAALLDKKPRPTDAEIDAAITNICRCGTYPRIREAIRKAARAQAGEERVAAAPP
                     PGIDPADAARTAPSLRGNRPAGSNGEGER"
     gene            8024..8761
                     /locus_tag="VF589_13190"
     CDS             8024..8761
                     /locus_tag="VF589_13190"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8258580.1"
                     /translation="MAATSVTPIASAAAAQNNNRQERREAREEREAREAREARQAREA
                     RQETREARQAREARQAREAQQARELSQGEQNRREGIRENRQERRGDIREYQEDRRERV
                     EDRRERVEDRRERVEDRQERREDVRDNRNDRRYTQRRDWSRTWNRGWRNDRRYNWQNY
                     RYTNRRIFSPGRYYAPYYGHNYNRLNIGLVLGSAFLSNRYWINDPWQYRLPPAYEGTR
                     WVRYYNDVILVDVYSGQVVDVIHGFFY"
     gene            8901..10169
                     /locus_tag="VF589_13195"
     CDS             8901..10169
                     /locus_tag="VF589_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8258581.1"
                     /translation="MLARFSALLLALLAAPAAAETVIVHAGRLITDASRPASGPATIT
                     IVDGRISSVTQGLTPAPAGARLIDLSQKTVLPGLIDTHTHLAGDPGGDYWSEAVDTDE
                     WAAMLGAKNARITARAGFTTVRDVGGPREVLFALRRATEQGILAGPRILTSGSSISII
                     GGHGDVSGFRPEVVAALQADNTCTGPEQCAQRVREASRDGADLIKITATGGVLSQQAR
                     GLGQHFTDAEMKAIVDTAHALGIKVAAHAHDARGIEAAARAGVDSIEHGTFADAKAIR
                     AMKASGTVLVPTLMAYTGIREGLARNAYTPAVAAKVRQALGEVGKAARAARAAGVPVV
                     FGTDSSVYEHGRNAEEFAQLVETVGMSPAEALASATTGAAKLLDRENEIGRIAPGFSA
                     DLIAVAGDPLRDVRALEKVDYVMVRGRTIE"
     gene            10186..10857
                     /locus_tag="VF589_13200"
     CDS             10186..10857
                     /locus_tag="VF589_13200"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8258582.1"
                     /translation="MELAAAAAAVRERLLARPGAVRIPAAGMELFGVADFLGEAECEG
                     LIALIDAGRKPSRVMDGHPDPEFRTSESCDLPPADRLVQAIEAKLAGLVGLDPAFGET
                     IQGQRYAVGQQFKPHHDFFSTGKPHWQVQKLAGGQRTWTAMIFLNTPEEGGHTLFPRA
                     QVKVAPLRGNLLVWNNLDDEGEPNLQTLHQGMPVVGGVKYIVTKWYRERRWTPHAARA
                     AAPPA"
     gene            11022..11525
                     /locus_tag="VF589_13205"
     CDS             11022..11525
                     /locus_tag="VF589_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8258583.1"
                     /translation="MRKLIILGLMAATALPSTAVLAQSRGEIRRDYRDLREEQRELNQ
                     ARRYGDRGDIREERRDVRDARRELREDISDRRNWGRNDWRSYRYSNRSLFSRGHYRAP
                     FRYRSFGIGYRIAPTYFGSSFFVDPYRYRLPYHGGYLRWVRHYDDVLLVDVRRGIVVD
                     VVRGFFW"
     gene            11681..13504
                     /locus_tag="VF589_13210"
     CDS             11681..13504
                     /locus_tag="VF589_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8258584.1"
                     /translation="MTRTLAILAAASSILALSATSSTAAPAPSAAPPAVAATDARQQL
                     HALFKWSDEENLRRNPLGALFRGDMRYADRLGDGLSDAYFAAERAADEEELRRLLAID
                     RSKLSTTDQIAYDVFRRQKEIGIRSTSPEMLALTAVRPLDHFFGFHTFYPDLASGQGV
                     APFKTLVDYENNLKRHHQYPVLIDRAIGRFREGMASGVVQPKLVVRNIIDQLELQLAQ
                     GVEGSTFYGPVKKFPEGISAADQTRLRAAHAAIIRDEIRPAYVRLRDFLKNEYLPVAR
                     EGEGLVHMKGGPQLYDFLIEQTTTLPLKADAVHELGLREVTRIKGEMEAIRNRVGFKG
                     DLAAFFQHLRTDPKFKPASKEWMTKRYYEIGKTVDARIGEIFSTLPKTPLEIRPVPAY
                     REKTDAGGSYNSGTPDGSRPGIFYYNTYDLPSRTTPGMETLYLHEGAPGHHFQISLAQ
                     ENEALPPFMRFGGNTAFAEGWALYAETLYDELGMETDPYQRFGGLDDEMLRAMRLVVD
                     TGIHSKGWTRDQAIQYMLANSSMGKTDATAEVERYIAIPSQALAYKVGQLKILELRAR
                     AEQALGNRFDLKRFHEQVLMTGALPLSVLEQKIDRWIASQA"
     gene            13551..15365
                     /locus_tag="VF589_13215"
     CDS             13551..15365
                     /locus_tag="VF589_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8258585.1"
                     /translation="MKHLPLLLAAAALAGCVETPPARTAATLPAAAAENAGEALAALF
                     AASDEASLERNPIQALFRGDLRYAGRLGHFLSDEYYAAERAAATRELAALAGIDRAAL
                     SPSERISYDVFKWQRELDRRGLEPDMLALTAVRPIDHFNGFHAQFAELSTGESIAPFK
                     TLQDYESGLTRIDDYVVNLDQAIVRMREGMAARVVQPRLVMENVVEQLDAMLAEGVEG
                     SSFMKPVATTPAEIPAADQVRLRTAYRQSVETEIQPALTRLRTFIVGEYLPATRASVG
                     LGAMPGGDRLYAYLVQASTTTDLTPDQIHRIGLDEVRRIQAEMEAVKQQVGFKGTLQQ
                     FFEHIRTDPKFKPTSREALQQGYVAIGKRLEATLPKLFRTLPRAPLDIRPVPALTEKG
                     AARGSYNPGTPDGSRPGIFYFNAYDLPSRTTPGMETLYLHEGAPGHHFQISLAQENEA
                     LPKFQRFGGNTAYVEGWGLYAETLGKELGVYTDPYQYFGYLDSQLFRAIRLVVDTGIH
                     AKGWTRDQTIKYIMANSSRGLSNATAETERYIANPGQALAYKIGQLKISELRTRAERA
                     LGPRFDLAEFHEQVLTSGALPLAVLERKIDAWIASKPG"
     gene            complement(15534..16217)
                     /locus_tag="VF589_13220"
     CDS             complement(15534..16217)
                     /locus_tag="VF589_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258586.1"
                     /translation="MRELGYVAVALVAAVSSPAAAQSPRELLVSAAFSARDKPTALAR
                     IDAALKGADAELARNPKNREAQLQRAVAIGYRGKLKRSRADVLAARRAFEALVAANPR
                     DAEAQMALAGWHLGAVIELGPLVARTALGARKSHGLQALDRAVALGGGRAFFPGYASL
                     NRILLDPGDIGRARALAEAAVKGRATASIDRVMQRQASALLQALRGGNGKSAARAAKG
                     LLPFGRLPN"
     gene            complement(16352..17959)
                     /locus_tag="VF589_13225"
     CDS             complement(16352..17959)
                     /locus_tag="VF589_13225"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX8258587.1"
                     /translation="MKVPQELRDAAMVAKAWPYEEARKILKRYPDGPGDRPILFETGY
                     GPSGLPHLGTFQEVARTLMVRHALTEMADWPSRLIAFSDDMDGMRKVPPGVPHQDMMH
                     EHLDQPLSRVPDPFGCGHRSYADHNNNLLRQFLDRFGFDYEFVSSSHCYSSGRFDETL
                     KSVLRNYTAIMDVMLPTLREERRQTYSPVLPLSPVTGKVLQVPIEVVDPNAGSVRYTD
                     PASGERVEQSILAGGAKLQWKVDWAMRWVALGVDYEMSGKDHIDGFIQSSRIARILGA
                     RPPEGFNYELFLDENGEKISKTKGNGVTIDEWLTYAPRESLSFYIYREPKKAKQLSLG
                     IIPKAVDEYYQILAAYPDQPWDKRLGNPVHHVHAGEVPETRLPISFALLLNVAGLPGV
                     GDRETIWSFLNRYNPALTAEASPELDRLVGYAVEYGSRFVAPALRRRAPTPEEAEALR
                     ELDALLAQGDTAGGPDEDPAVILQNHVYEIGKQHYGKERLRDWFKALYETLLGSEQGP
                     RMGSFIALYGVDNSRRLIAEALAEEAG"
     gene            17997..20723
                     /locus_tag="VF589_13230"
     CDS             17997..20723
                     /locus_tag="VF589_13230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX8258588.1"
                     /translation="MAASLPYPALHATHGGVWLAAPDGAVTALGRGEAIARAAETPLI
                     MLNAPLVGQRLGYPDLSGLDLLELFAFVFPARFTVPTPKGLAQALGLAPPKDEAGAAP
                     FLLRAAAALLDRMGAADWPEREGAWASAQALYRLRWPWAPAIAERLKRPERDERWLFS
                     RLTEWEETPPRPQPRPVRLVPEEAVERLAGLTGRGAEPRQGQRDYAAAAAAAFAPRNV
                     EGRPNLLLAEAGTGIGKTLGYLAPASLWAAKAGGAVWVSTYTKALQRQLDREGARIFP
                     DEAERRKKIVVRKGRENYLCLLNLEDALQGGFAGRAAILAQLVARWAAYTRDGDMVGG
                     DMPGWLPTLFRRAGATALTDRRGECVYAGCPHYRKCFIERSARASQDADIVIANHALV
                     MVNAARGREMGNAPGRIVFDEGHHLFDAADSTFAAALTGQETIELRRWLIGPEGKARG
                     RRRGLAARLTDVASYDEAGAAALEAAVQAAGLLTGDGWLQRVAENMAFGPLERLLAAV
                     RGTVYARASAQDAGYGIETEIADPDPALVEAAAPAVEAMELLLRPLVALGKRLEAVME
                     DAPDWLDAQARARIEGAIGGLAWRVQSLSAWIALAARIGGPADPDFVDWLAVERIDGR
                     EYDIGIHRRWLDPTRPLAETVLKPAHGIIVTSATLRGAGDWAAAEARTGAAHLDHPAQ
                     RFSADSPFDYAANSEVLIVTDLKRGDLAQLAGAYARLIEAAGGGTLGLFTAIQRLKAV
                     HARIADRLARAGLPLYAQHVDPIDTGTLVDIFRDDPRASLLGTDALRDGVDVPGHSLR
                     LVVMEGVPWPRPTVLHAARRAVGGGTAYDDRVVRARLAQAFGRLIRRKEDRGAFVLLS
                     SATPSRLLDAFPPGVAVRRVPLAEALERVRSSLSTVTSLGHQGAAFAPEETR"
     gene            20720..21259
                     /locus_tag="VF589_13235"
     CDS             20720..21259
                     /locus_tag="VF589_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8258589.1"
                     /translation="MKTLTLLRHAKSSWNDPVARDFDRPLNTRGRKAARVVGAEMRAL
                     GLVFDRVLASPAVRVVETLEEVARGYGAALAPVYDKRIYLASPGALLDLIREVDGADS
                     LLVVGHNPGMEQLALALAGEAAGDCRSELALKYPTAALAEIRLPADRWSEVEEGAGFL
                     ARFVRPRDLDPELGPDEDS"
     gene            complement(21354..21779)
                     /locus_tag="VF589_13240"
     CDS             complement(21354..21779)
                     /locus_tag="VF589_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8258590.1"
                     /translation="MTQLPPFHLAFPVHDLDAARGFYGGLLGCREGRSAAHWIDFDFF
                     GHQIVAHLSPEARPRAHHNPVDGHDVPVPHFGAVLGMAEWRALAERLRAAGTEFVIEP
                     DIRFAGQPGEQATMFFLDPSGNAIEIKAMAKPENLFATE"
     gene            complement(21776..22453)
                     /locus_tag="VF589_13245"
     CDS             complement(21776..22453)
                     /locus_tag="VF589_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258591.1"
                     /translation="MVIAFLYLIAAQASQPVLPAREADAGLRCTAAFMALADAQKPAF
                     VPAIGDPALVDRAMAFTLLTRQKLARGPEPRQSAVDRAMAALAEVQGAAKRSGGGDAY
                     FRAEIRRCRPLIENALSEEAERLSIAPAELPLDYLRCVVVYAMGYGVAFRPETEQGRY
                     GNEAARKFEQLYTKSRSLAAAGAEEWQQIWARDMQTIEAARESGTTRRDESLHCVLLA
                     IRSEAKP"
     gene            22635..24215
                     /gene="lnt"
                     /locus_tag="VF589_13250"
     CDS             22635..24215
                     /gene="lnt"
                     /locus_tag="VF589_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX8258592.1"
                     /translation="MKFLGLSFLAGVVSALGFAPLGLWPLTLAAFTALLWLIETAPGL
                     RAALARGYWFGVGQFVLGLNWIATAFTYQAAMPAWLGWLAVVLLSLYLAVYPAMAAGL
                     AWRWGSVGNISTRHSRESGNEASSSAPNPPPALTLILVLAAAWIVTEWLRATMFTGFA
                     WNPVGVALLPTPASRLAAWVGTYGLSGVAILLAGVLLLLARRAFRPAGLLLAAVAVPS
                     AALLLLGGAPAGAGPPIRIVQPNIGQEERWREEVIEENFAKLERLSKSAARGPRLLLW
                     PESAVVEPLEEERADPYRQERVLQVRERLGQMLGPEDLLLAGGVAFRSTDGANLTSAA
                     NSVYAVDTAGRLIGRYDKAHLVPYGEYLPMRPILSAIGLSRLAPGDVDFDSGPGPRTL
                     DLPGFGKVGLQVCYEIIFSGEVVEDGDRPSFLFNPSNDAWFGAWGPPQHLAQARLRAL
                     EEGLPVLRSTPTGISAVIDAEGRLLHSIPWRTAGVIDARLPAAKAPTLFARAGNILPF
                     LFALALAAIAIAVRRKHV"
     gene            24284..25504
                     /gene="metK"
                     /locus_tag="VF589_13255"
     CDS             24284..25504
                     /gene="metK"
                     /locus_tag="VF589_13255"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX8258593.1"
                     /translation="MRSNYLFTSESVSEGHPDKVADQISDAVVDLMLSKDPEARVACE
                     TMVTTQRILLAGEVRCRGVFDLESFPDTEGWAPGSRDEIEQAVRRTVEKIGYEQDGFH
                     WQTAYFSNHLHGQSQHIAQGVDESGNKDEGAGDQGIMFGYATDETPDLLPATLYYSHR
                     ILERMASDRHNKVVDFLEPDAKSQVTLQYENGVPVRAAALVVSTQHSPALSNDAGQAR
                     LREYVKGVFADVLPEGWLPADEAIHVNPTGRFEIGGPDGDAGLTGRKIIVDTYGGAAP
                     HGGGAFSGKDPTKVDRSAAYVSRYLAKNVVAAGLAKRCTIQLSYAIGVAEPLSLYVDL
                     HGTEANGTTAARLEQVLPTLVRLTPKGIRTHLGLNKPIYGATAAYGHFGRKAEGDFFT
                     WEKTDLVDRLRAAI"
     gene            25619..26299
                     /locus_tag="VF589_13260"
     CDS             25619..26299
                     /locus_tag="VF589_13260"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEX8258594.1"
                     /translation="MPDPTTIRRLYGRRSGHKLRQGQAALLEELLPAISVPDAGPITS
                     AALFGDDRPLHFEIGFGSGEHLAYRADLLPDHGFIGAEPFINGVVGALQHVRDRQLAN
                     VRLHLGDALDILERLPDSSLRFVYLLHPDPWPKARHAKRRMVNPGPLDLIAAKLQPGG
                     EFRLGTDDPVYCRWSMMVMSRRGDFEWLAESAKDFLTRPGGWAETRYEAKARRKGHEV
                     WYFRYRRV"
     gene            26299..26772
                     /locus_tag="VF589_13265"
     CDS             26299..26772
                     /locus_tag="VF589_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258595.1"
                     /translation="MRTIVLGLMLLAGVEGAALACSCIPPGAPEQSRGFAREALQGVT
                     AIVEAEAISDYRPGGPGEEVRVLRTLFGSAPPTIRLERSEHASSASCDLLLGKGERKV
                     LMLTPAEGGTYRMQSLCSDYLTSERYLPILLEEAGWTSKAGERAERCGGGISAPA"
     gene            complement(26756..27892)
                     /locus_tag="VF589_13270"
     CDS             complement(26756..27892)
                     /locus_tag="VF589_13270"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8258596.1"
                     /translation="MRAILLAALPALLTVSCDATEAGTAGAPKASASRPFAVEDIARF
                     DEPWAMTFLPNSGQALITEKKGRLMLWNEGGLVSPVKGVPKVDYGGQGGLGDVIVHPD
                     YANNNLIYLSWVEAGDGDTRGAVVGRAKLVAGESGRLEGLQVIWRQHPKVEGRGHYGH
                     RLAFGPDGKLYISNGDRQKFTPAQDMSATLGKVVRLDDDGSVPADNPWASRGGVTAQL
                     WSAGHRNILGLAFDGQGQLWSSEMGPKNGDEFNRIERGSNYGYPIVSNGSHYDGKDIP
                     DHSTRPEYNAPEITWNGLSPAGMMIYSGSLFPAWRGNAFLGGLSGEALVRVQMTGTGA
                     REAERWDMSARIREVEQGPDGSIYVLEDEDDGEGGRLLRLRPAR"
     gene            27995..29056
                     /locus_tag="VF589_13275"
     CDS             27995..29056
                     /locus_tag="VF589_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX8258597.1"
                     /translation="MIQNAHSSVALLSLATAAPDIVLPQEEAKALAARLFGRRTSLFE
                     RLAPVFDNAGIDRRRIVKPPAWYEAEHGWRERNRVYLEAAEALYVEAAQKALGLAGVA
                     AHEVDGLVTVSTTGIATPSLEARAAGRMGFRKDAQRVPVFGLGCAGGVTGLGLAARLA
                     AGEPGRTILYVTVELCSTSIRLDKLSKANIVATALFGDGAAACVLRAGEGGVGAIGPG
                     AELLWPETLGIMGWDVEDPGLAVIFDRSIPPFVEEHMAEAVATIAAKAGISPRDISRF
                     ACHPGGAKVLTALEGSLGLAEGALDHERAVLREYGNMSAPTVLFVLERLLAEGLPERT
                     MLLALGPGFTASGLLLTAR"
     gene            29053..29544
                     /locus_tag="VF589_13280"
     CDS             29053..29544
                     /locus_tag="VF589_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8258598.1"
                     /translation="MTAAIVILTLVTLQRLAELLVARRNTRALLAEGGHEVGAGHYPL
                     IVAVHGLWLAALWWLAPGRAIVWPLIGLFLLLQAGRLWVLATLGRRWTTRIIVLPGAP
                     LVTSGPFRFVRHPNYLVVAGEIAVLPLAFGLWKLALLFSVLNAAILVIRIRAENGALS
                     DPR"
     gene            complement(29541..>30613)
                     /locus_tag="VF589_13285"
     CDS             complement(29541..>30613)
                     /locus_tag="VF589_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8258599.1"
                     /translation="PAPHVRDTLARRVFRATAVVIATLTAAYLLWVLVDLLLLLFACA
                     LVSLILLTITNSIRRRTRLPFAVALTATVLGLLALIGGAFAFFGATLQGEFAELATRL
                     PAAWADFQARLQTTPIGAAILQRAQGLAPSGQAIVSAVTATLTAVGGAVSGLLIVLIG
                     GLYLAAQPTLYSHGLLRLFPARAQAPAIETFDAVAVSLRNWLKGQALGMVFVGLATGF
                     GLWLVGLPAAWAIGLVAGLAEFVPYLGVLVAGIPAVILGFGQGTDTGLWTVGVLIAVQ
                     QLQGNLVMPLLQNRMVDLPPAVTIFGIIAAGILFGVAGVLLATPLTIVVLVLVRRLYL
                     HEDKHEVLASADAPPAPPPPAT"
BASE COUNT         4679 a        10830 c        10248 g         4856 t
ORIGIN      
        1 gccagatcga ggccgcggtc cgcctgcgcg cgccggtggt cgagctccat accggccgct
       61 acgctcatct cgaaggcgag gcgcgggccg acgagctgcg ccggctggcc ggcgccgccg
      121 cgctcgccgc caagaacggc atcgaggccc atgccggcca cggcctcagc ttcgccaacg
      181 tcgtgccggt ggcggcgatc ccgcagctcg ccgagctcaa tatcggccat ttcctggtcg
      241 gcgaggcgat cttcaccggc ttcgaggcga gcgtgcgccg catgcgcgac ctgatggacg
      301 aggcgcgcgg tgcctagctc ctccccggca cggggagggg gaccgcgccg cggagcggcg
      361 tggtggaggg ggctctccac ccgcagcggc ctgcatcttc ccccctccac cgtccgccgg
      421 tggcggccgg tcccccaccc cctgccgggg aggaatatat gatcatcggc atcggctcgg
      481 acctgtgcaa catcgagcgc atccaggcct cgctcgaccg cttcggcgag cgcttcatcg
      541 cccgcgtctt caccgagacc gagcgcgcca aggccgagcg gcggccgttc acccgcgccg
      601 gcacctatgc caagaggttc gccgccaagg aggcctattc caaggcggtc ggcaccggct
      661 tccggcgcgg cgtgttcatg aaagatatag gggtggtcaa taaaccctcc ggcgaaccca
      721 ccctccacct caccggcgga gccaaacaga ggcttgacga catgacgccg gaaggccacg
      781 ccgccgaggt ccacctgacg atgaccgacg accatccgtt cgctctggcc ttcgtcatca
      841 tcaccgccgt tcctctcaga gacccggcga cccaaggagc aagagggtga gcgccagtga
      901 acccgccgtg ccgatgaccg agaagagcgt gaagaccaag cccaagggcc agaccgactg
      961 gtggagcgag atcaagggca tcttctggct catcctggcc gtgctcgctt tccactcgtt
     1021 cgtggccaag ccattctaca tcccgtcgga atcgatgatg ccggtgctgc tcaagggcga
     1081 tcggctggtg gtcagcaaat atccctatgg ctggtcgtgg gtcagcccca gcttccacgt
     1141 gctcccgcaa atgggcgggc gcctgttcgg ctcgctgccc gagcgcggcg acatcgtcat
     1201 cgccaccccg cccgggcaga gcgccgacta tatcaagcgc gtggtcggcc tgcccggcga
     1261 cgagctcgag atgatcgacg gccagctcat catcaacggc ctgccggtcg agcgcgcgga
     1321 gcagccggcg cggatggtgc cggtcgacga caacgcgccc tgcctgccga ccgaattccc
     1381 cgacgcccgc gtcaagggcc gcgacggcaa tgcctattgc cgcctgccgg tggtgcgcga
     1441 gacgctgccc aacggccgct cctacgacac gatcgatctc ggctattcga ccactgacga
     1501 tttcccgaag atcaaggtcc ccgattacca cgtcttcctg atgggcgaca atcgcgaccg
     1561 ctcggccgac agccgggtcg cgctcgacca gcaggggctg ggcgggccga tcccctatga
     1621 gaatatcggc ggccgcgccg agttcatcac cttctcgctc gacggcacct ctcaatatct
     1681 gaacccgatc agctggttca cggcgcttcg cggcgaccgc gccggctcga cgctccaccc
     1741 aacccaagga gcgcccacgg agtgaccgag gacggcgagg ttcacgtcga gcgccgaggc
     1801 ccgaccgaac ttcgcgatcc gatgatccgg caggagctga agcgggcctc ggtctggttc
     1861 ggcatcggcg tcgggctcct cgctctgttc ttcctcgccc agccgctcct gctcatcttc
     1921 gccggcatcg tgctcgccgc catgctcgac ggcggcaccc gcctgctcgg ccgggtgctg
     1981 ccgatcgggc gcggctggcg gctggcgatc gtcaccatcc tcgccatcgg cttcgtcgtc
     2041 tggaccttcg tcttcgccgg ccaaaccatc gccgcgcagg cggagtcgct gcgcaccgcg
     2101 gtcaccttcc agctcaaccg catcctcgcc tggggcaacg agctcggcat cgtccagggc
     2161 ggcgccggca tccagcagct cagcggccag ctgatgggct cactcgggcg attgggctcc
     2221 gcagtcagca gcgcgctcgg cgccttgacc agcatggtga tgatcctggt gatcggcatc
     2281 ttcatcgccg tcgaaccgcg gctctacgag cgcggcttcg cctggatgct gccgatccgc
     2341 gggcgggacg aattctaccg caccgcctcg catatgggcc gcacgctgcg gcggctgatg
     2401 gccggccggc tgatcggcat ggcggtcgaa ggcttcggca cctggctgct gctgtggatc
     2461 ggcggcgtgc cgatggcggc tctgctcggc atcctcaccg gcctccttgc cttcatcccc
     2521 aatgtcggcg cgatcgtgtc gggggtgctg atcgtcctcg ccgggttcag cgtcagcacc
     2581 gacgccggca tctgggcgat cggcgtctat ctcatcgtgc agaccgtcga tggctatctg
     2641 atcgtgccct atgtcgcccg caagacggtc gacctcgcgc cggccctggt gctgggcgcg
     2701 cagctgctgt tcggcgcttt gttcggcatc atgggcctgg cgctcgccga tccgatcgtg
     2761 gccatgatca aggccgcgct cgaacagaaa tcgcgggagg aaagcgggct ggccgcgggc
     2821 gtggccgagc cctgaggctt cagagcccgg gcggaagctg cccggccgcc tgcgcggcgg
     2881 cgttcggcac gacctggagt atgctgacgt cgaaatcgag caccgcattg ggcgggatcg
     2941 gcccgcccgg agtgccggcc tcgccataag cgagctgcgg cggcagccag atccgatagg
     3001 cgccgcccgt ttgcatcagt tgcagcgcct cgctgaagcc gggcacgacc tggccgacca
     3061 gcatcggagc cggacccttg ccctcggtcg aatcgaacac ggtgccgtcg agcagccggc
     3121 ccttatattc gatgatcaca ccgtcgttgg cgccgatcct ggcgccggag ccggggcgga
     3181 tagtctggat ctggaccccc gaggcggtgg tggtgacgcc ctcgcgcttg ccgttcgagg
     3241 cgaggaagcc ggccggatcg cccatcgcca cctgcgtgct ggtgcccgcc caggcgatgc
     3301 ccgccgcgcc gagcgccagc gcgccgagtc caacccagag cttgacgacg gtgcccttct
     3361 tgaccgggcg gatcgggacg gcggttacgg acattggctt ctcactcggg ttaggcggcg
     3421 cggcgatcta tcgccgtccg ggtcatggaa gcaagcccgc gaccgcccag ctcccgtcac
     3481 cctgaacacg tttcagagcc tgccccggac ctgatccggg ggtccatccc gtcacccgcg
     3541 agaggcggag aagtggacgc cgaaacaagt tcagcctgac gcccgcaggt ttacgaaggt
     3601 cctagcggac cccgtcgcgc tccatgcgct tgcgctcgag cttgcgggcg cggcggacgg
     3661 cggcggcgcg ttcgcgggcg cgcttctccg acggcttctc atagtgacgg cgcagcttca
     3721 tctcgcggta gacgccttcg cgctggagct tcttcttgag cgctctcagc gcctggtcga
     3781 cattgttgtc gcgaacgatg atctgcataa agtctcgtca ctctccaatt gctcaggcgg
     3841 cggccggctc gagcgccgcc ttctgaaaat ccagcgatac cataaaaatg ggttcgccgc
     3901 gagacggttc ccgcgacgtt ggcgcgccgg ttagcagggc gcgccgccca tagcaagcgg
     3961 gcgcggagcg cgccggcgag tcggcccttc atgataggac gaaacccgcc gccgccaaca
     4021 ttattcccgc agccgccttg gcgaaacgga tggccgcggc gcggggccgc ggccggcatc
     4081 gcggaagccg cagccggggc gctgactgcg cccctgcgaa ggcaggggcc cagaccagcc
     4141 cctgcgaagg caggggctgt cggacgagcg tggggtagag gtcgcgccag tcgggtttgt
     4201 gccgttgagg ccgctccgat tggtgtaaac atatcggcct tcatccaggg aggaccgggc
     4261 cggcgctaaa gcgccagccc gggtctggac ccctgccttc gcaggggaac cgtgcggcac
     4321 tcgcgccccg gcgaaggcag gggtccagac cagcccctgc gaaggcaggg gctgccggac
     4381 gggggcggcc gggggagggc ggtcgtggaa cgtacaaaga actttcctac aggtcgccga
     4441 atcgcggata gaataaggat gacaggatcg cagctgctcc cgccgcgcct cctcgccctc
     4501 gtccggcaca ggctggtcgg ggtgctcgcc ttgctgccgg tgccggttcg cgcccgccgc
     4561 catcactgga ccgccgggag cccgcgccac cccatcccgc ggctaccggc tggcggccgc
     4621 gccgccgccc gcccggcggc ggccggcctc gacgagggct ctgccgaccg ttcgacgaag
     4681 cggctgccgt cactgaaaac aaaggagttt tccggcgtat atgggggtat ttggtgtact
     4741 ttcgacgcgt ccgcggcctt gcttcctccc cggcccctat ccgtcctggc cgaggttcgg
     4801 ggcggacacc gcgacgaagt cggcggaggt gaggatcgtc cccacgtcga cggtggcgaa
     4861 cagcaccgcc accgcggaac cgccgctgcc gtcggcgtcg tagaagatcc tgccggtcgc
     4921 ctgatggtag acgatccggt cgctggcgtc ggtcgcctcg gtgccggcgc ggaaggcgct
     4981 cgccgccagc cgcccgtcgg ccgcgatcgc gtcgaagatc gcgcggtcga gcaggatcgt
     5041 gtcgtcggcc gccacgaaat cctcgatcgc gtcgatattg tcggtgccgt cgatgccgct
     5101 gtcgaacctg aaccggtccc ggccggtgcc gccggtcagc gagtccgcgt cgtcgccgcc
     5161 cgagagccgg tcgtcgccgc ccccgccgtt cagccggtct tccccgtcgc cgccatagag
     5221 acggttcgcg gcggtgttgc cgaggatgct gttggcgagc ccgttgccgc tgccgatcgc
     5281 cgccgtgccc gtcaggacca ggctctcgag attgtcgtag agataatggt tttccgaagc
     5341 gatcacccgg tcgctgccct cgccggcccg ttcgctcacc cggtcgtccg agccgccgac
     5401 gatgtagatg tcgttgcccg cgccgccgta aaggttgttg ctgccgccgc cgccgtcgag
     5461 ctggtcgttg ccggcgccgc cgtacagatc gtcatattcg tcgccgccga ccaggatgtc
     5521 gttgccgcct tgcccgtaga gatagttgct ctcggcatac tcgctctcat cctcgatttc
     5581 ggcctgatcg ccgatggtca cggtgggatc gaagctggtg acgccggggg cgacggcgct
     5641 gcccgggtcg aagtcgccga acagccggtc gtcgccgccg ccgcccatca gccggtcgcc
     5701 gccttcgcgc ccttcgatcc ggttggcgac cccgttgcca tggacggcgt cgccatttct
     5761 cgagccgatg acgttctcga tcgacacgaa cgtgtcgccg tcgccctggt ccatgccttt
     5821 ggcgccggtg gcgagggtga tgctgaggtt attggaggcg tcggcatagc aggccgtgtc
     5881 gatgccgagg ccgccgtcga gcgtgtcgcc gagcgcgccg cccatcagca catcgttgcc
     5941 gtcgccgccg accagcaggt ccatgccggc cccgccgtcg agccggtcgt tggcgccgcg
     6001 gccgtcgagg agatcgtcgc cggtcaggcc caccaaggta ttgacgaggc cgttgccggt
     6061 gatctggtcg gaactgtcgc tgcccatcgc gctttcgatc cgcatcaggg tggccatgac
     6121 ggcgccgtcg ctggcaaatc cggcagcgag gtcgataatc atcgcgccgg cgaagccgat
     6181 gccgccgaag gcgacgaaat cgttggtgcc gccgccgccg tccacgcgaa tgggcatgtt
     6241 gctgcagtag aatacctcgt tcgccgccga gccggccatg atcgcgccca tgcccgggct
     6301 gagataatgt tcgacgccta ccagctcgtc gatctggtaa gtgacctcgc tgagatctcg
     6361 tatcgaatcg gcgttgccgt cggtccacat ctcgtagaag gcgacgcccg attgcggcga
     6421 cgacaaggtg atccctttga tcgccgcgag gtcgatggcc cggttgccat aggcaatctc
     6481 gaaatcgacg tcgtcggggc cgatcggcat ggccgcgtaa tagatgtcgg tcccgccgcg
     6541 gccattgtag aaatcgtcgc cgtccgcgtc ctggaagccc tcattctggt tgctgccgag
     6601 gaaccggtcg ttgccggagc cgccgcgaaa ctgctcgatg ctgcgaagcc ggtcgccggc
     6661 cgcgtggccg gcattggcgc tctggttcac aaggtcgacc accgtggcca tgtcgccgtt
     6721 cacataatac cagtcggagc cgacgccgcc gtcaagcgtg tcggcctccg tgtcataaac
     6781 ctgaaagctg tcgtcgccgg cctccccgaa cagcgcatcg cccgcgtcgc catcggccaa
     6841 gtcatagagg acgtcgtggc catcgccgcc atagaggcgg tccgccccat gtcctccgtc
     6901 caggaaatcg gtgccgccgc gccccgcgat ataatcgtcg ctgctggttc cgttgagcat
     6961 gtcggtgccg ccggtcccgt tgatgaaagc catggccctt ctccttttcc cctaaatgtc
     7021 ggccggccgg tcagcgcctt cggccgcgaa cgcgcgcgcg tcggcgccgc ctcggtcggc
     7081 ggcggcggac aagcgcggat atcggatcag gctcaggctg gacttcacga tcgccatccc
     7141 cctgttcccg aggcgactcg taagtaacgg gccaccctac actcgcgcgg gaggggcgtc
     7201 cacgcgctta tcaggccaag tctttcaact aactggcgtt ccgcaactgt ccggcatctt
     7261 cgacctattc ggcgtcttcc aactgtgcgg ctggcgctct agacccggcc catgtcgcgt
     7321 ttcaccgtca acggccagcc gatccattac cggctcgatt ccgatacgcc cctgctctgg
     7381 gcgctgcggg acgcctccaa cctcaccggc accaaatatg gctgcggcac cggccattgc
     7441 ggcgcctgca ccgtcaatat cgacggcaat gcggtgcgca gctgcctggt caggatcggc
     7501 aatctcgaag gcagcttcgt caccaccatc gaggcgctgt cgcgcgaccg ctcgcatccg
     7561 gtccagcagg cgtgggtcgc cgatcaggtg ccgcaatgcg gctattgcca gtcgggcatg
     7621 atcatggcgg cggccgccct gctcgacaag aagccgcggc cgaccgacgc cgagatcgac
     7681 gccgccatca ccaacatctg ccgctgcggg acctatccgc gcatccgcga ggcgatccgc
     7741 aaggccgcgc gagcgcaggc gggggaggag cgggtcgcgg cggccccgcc gccgggcatc
     7801 gaccccgccg acgcggcgcg cacggcgccg tccctgcgcg ggaacaggcc ggccggatcg
     7861 aacggcgagg gggagcgtta atgaagctgt ctgatagctt gcaatagggt tcagcaacgg
     7921 ctcatccggg atgtcgcaca tgtcgcagca ccccggaggc ggccttaccg ctttcgaccc
     7981 gaacggagtg aaatcgatgc gtagagcaat tatgggtatt ttgatggcgg cgacttctgt
     8041 cactcccatc gcgtcggccg cggccgcgca gaacaacaac cgccaggagc ggcgcgaggc
     8101 ccgcgaagag cgcgaggccc gtgaagcccg cgaggctcgc caggcgcgcg aggcgcggca
     8161 ggagacccgc gaggcacggc aggcccgcga ggcacgccag gcgcgcgagg cacagcaggc
     8221 gcgcgagctc agccagggcg agcagaaccg ccgcgaaggc attcgcgaga accgccagga
     8281 acgtcgcggc gacatccgtg agtatcagga agaccgccgc gagcgcgtcg aggaccgccg
     8341 tgagcgggtc gaggatcgcc gcgaacgcgt cgaggaccgt caggagcgcc gcgaggacgt
     8401 tcgcgacaat cgcaacgacc gccgctacac ccagcgccgc gactggagcc ggacgtggaa
     8461 ccgcggctgg cgcaacgatc gccgctacaa ttggcagaat tatcgctaca ccaaccggcg
     8521 gatcttcagc ccgggccgct attatgcccc ttattacggg cataactaca accgcctgaa
     8581 catcggcctg gtgctcggct cggcgttcct gtcgaaccgc tactggatca acgatccgtg
     8641 gcagtatcgc ctgccgccgg cctacgaggg cacccgctgg gtccgctact ataacgacgt
     8701 catcctggtc gacgtctaca gcggccaagt cgtcgacgtg atccacggct tcttctacta
     8761 agaccaattc ccttcgggga tgcgccaatt cccttcgggg atgcaaaggc ccggggcgtc
     8821 ccagcgacgc tccgggcttt ttgcttgtaa tcaaagcgta agctggagca gatcggcgcc
     8881 tgccatttcg ggggtccgcc atgctcgctc gcttctccgc cctgctcctc gccctcctcg
     8941 ccgcgcccgc cgcggccgag accgtgatcg tccacgccgg ccgcctgatt accgacgcct
     9001 cccgccccgc ctccggcccc gccaccatca ccatcgtcga cgggcggatc agcagcgtca
     9061 cccagggcct cacccccgcc ccggccggcg cgcggctgat cgatctttcg caaaagacgg
     9121 tgctgcccgg cctcatcgac acccacaccc acctcgccgg cgatcccggc ggcgattatt
     9181 ggagcgaggc ggtcgacacc gacgaatggg cggcgatgct gggcgccaag aacgcccgca
     9241 tcaccgcgcg cgcgggcttc accacggtgc gcgacgtcgg cggcccgcgc gaagtgctat
     9301 tcgcgctccg gcgcgcgacc gagcagggca tcctcgccgg cccgcgcatc ctgacgtcgg
     9361 ggtccagcat ctcgatcatc ggcggccatg gcgacgtcag cggcttccgg ccggaggtcg
     9421 tcgccgcgct ccaggccgac aatacctgca ccgggcccga gcaatgcgcc cagcgcgtgc
     9481 gcgaagcctc ccgcgacggc gccgacctca tcaagatcac cgccaccggc ggcgtgctgt
     9541 cgcagcaggc gcgcggcctg gggcagcatt tcaccgacgc cgagatgaag gcgatcgtcg
     9601 ataccgccca tgcgctcggc atcaaggtcg cggcccacgc ccatgacgcg cgcggcatcg
     9661 aggcggcggc gcgggccggc gtcgattcga tcgagcacgg caccttcgcc gatgccaagg
     9721 cgatccgggc gatgaaggcg agcggcacgg tgctggtgcc gactttgatg gcctatacgg
     9781 gaattcgcga gggcctcgcc cgcaatgcct atacgccggc cgtcgccgcc aaggtgcgcc
     9841 aggcgctggg cgaggtcggc aaggcggcgc gggcggcgcg ggcggcgggc gtcccggttg
     9901 tgttcggcac cgattcgtcg gtctacgaac atggccgcaa tgccgaggag ttcgcccagc
     9961 tcgtcgagac ggtggggatg agccccgccg aggcgctggc ctccgccacc accggcgccg
    10021 cgaagctgct cgaccgcgag aatgagatcg gccgcatcgc ccccggcttc tccgccgatc
    10081 tcatcgccgt tgccggcgat ccgctccgcg acgtgcgcgc gctcgagaag gtcgattacg
    10141 tgatggtgcg cggccgcacg atcgaatgag cggagggctt gtgaggtgga gctagcggcc
    10201 gcggcggcag cggttcggga gcggctgctc gcccggcccg gcgcggttcg catccccgcg
    10261 gccgggatgg agctgttcgg agtggcggac ttcctcggcg aggccgagtg cgagggcctg
    10321 atcgccctga tcgacgcggg ccgcaagccc tcgcgggtga tggacggcca tcccgacccg
    10381 gaattccgga ccagcgagag ctgcgacctc ccgccggccg accggctggt gcaggcgatc
    10441 gaggcgaagc tcgcgggcct cgtcggcctc gatccagcct tcggggagac catccagggc
    10501 cagcgctacg cggtcggcca gcagttcaag ccgcaccatg atttcttcag caccggcaag
    10561 cctcactggc aggttcagaa gctggccggc ggccagcgca cctggaccgc gatgatcttc
    10621 ctcaacacgc ccgaagaagg cggccacacc ctgttcccgc gcgcccaggt gaaggtcgcg
    10681 ccgctccgtg gcaatctcct cgtctggaac aacctcgacg acgagggcga gccgaacctc
    10741 cagacgctcc accagggcat gccggtcgtg ggcggcgtca aatatatcgt caccaaatgg
    10801 tatcgcgagc ggcgctggac cccgcacgcc gcgcgcgcgg ccgctccccc ggcctgatcc
    10861 taagtcgatc gcgcatcgcc gacagcagca gcagcggctt tcaatttgct gactagcgca
    10921 ttcccgcagg gttcagccgg gccatggcaa aagcgaatca tcggtgcggt ccccgagtcg
    10981 ctttttgggg gatcgcgcct aaagctcgaa aggactttgt gatgcgtaaa ttgatcatcc
    11041 tgggcctgat ggccgcaacc gctctgccgt cgaccgccgt gctggcccag tcgcgcggcg
    11101 aaatccgccg tgactatcgc gatctgcgcg aagagcagcg cgagctgaac caggcccgcc
    11161 gctacggcga ccggggcgac atccgcgagg agcgccgcga cgtgcgcgat gcccgccgcg
    11221 agcttcgcga ggacattagc gaccgccgca actggggccg caacgactgg cgcagctacc
    11281 gctacagcaa ccggagcctg ttctcgcgcg gccattaccg ggcgccgttc cgttaccgca
    11341 gcttcggcat cggctaccgg atcgccccga cctatttcgg ctccagcttc ttcgtcgacc
    11401 cctatcgcta tcggctgccc tatcatggcg gctacctgcg ctgggtccgt cactatgacg
    11461 acgtgctgct ggtcgacgtc cgccggggca tcgtggtcga cgtggtccgc ggcttcttct
    11521 ggtaagaagt ccgactatcg gaacatgccg accctgaaga ggcggcgcgg aggttttccc
    11581 tttctccgcg ccgccttttt cttcgcctcc ctttcggcgc ttgcagcgcg ccgcccggcg
    11641 tcttagacga ccaacacagt ttagctgaaa ggtcgttccc ttgactcgca cgctcgccat
    11701 cctcgccgcg gcatcctcaa tcctcgcgct ctccgccacc tccagcaccg ccgcgcccgc
    11761 gccctctgcc gcgccgccgg ccgtcgccgc gaccgacgcc aggcagcagc tccacgcctt
    11821 gttcaaatgg tcggacgagg agaatctgcg ccgcaatccg ctgggcgccc tgttccgcgg
    11881 cgacatgcgc tatgccgacc ggctcggcga cggccttagc gacgcctatt tcgcggccga
    11941 gcgcgccgcc gacgaagagg agctgcggcg cctgctcgcg atcgaccgct cgaagctctc
    12001 gaccaccgac cagatcgcct atgacgtctt ccgccgacaa aaggagatcg gcatccgctc
    12061 gacctcgccc gagatgctgg cgctgaccgc ggtccggccg ctcgaccatt tcttcggctt
    12121 ccacactttc tatcccgatc tcgcctccgg gcagggcgtg gcgccgttca agacgcttgt
    12181 tgattacgag aacaacctga agcgccacca tcaatatccg gtgctgatcg accgcgcgat
    12241 cggccgcttc cgggaaggca tggcgtcggg cgtggtccag cccaagctgg tcgtccgcaa
    12301 catcatcgac cagctcgagc tgcagctcgc ccaaggggtc gagggctcga ccttctacgg
    12361 gccggtcaag aaattcccgg aaggcatctc cgcggccgac cagacgcgct tgcgggccgc
    12421 gcacgccgcc atcatccgcg acgagatccg cccggcctat gtgcgcctgc gcgacttcct
    12481 caagaacgaa tatctccccg tggcgcgcga gggcgagggc ttggtccaca tgaagggtgg
    12541 cccgcagctc tacgatttcc tgatcgagca gaccacgacg ctgccgctca aggccgacgc
    12601 ggtgcacgag ctcggcctgc gcgaagtgac gcgcatcaag ggcgagatgg aggcgatcag
    12661 gaaccgagtc ggcttcaagg gcgatctcgc cgccttcttc cagcatctgc gcaccgaccc
    12721 caagttcaag ccggcgtcca aggagtggat gaccaagcgc tactacgaga tcggcaagac
    12781 cgtcgacgcg cgcatcggcg agatcttctc gaccttgccc aagaccccgc tagagatccg
    12841 gccggtgccg gcctatcgcg agaagaccga cgccggcggc tcctataata gcgggacgcc
    12901 ggacggatcg cggccgggca tcttctacta taacacttac gacctgccgt cgcgcaccac
    12961 gcccggcatg gagacgctct acctccacga gggcgcgccg ggccaccatt tccagatcag
    13021 cctcgctcag gagaatgagg cgctgccgcc gttcatgcgc ttcggcggca acactgcctt
    13081 cgccgagggc tgggcgctct atgccgaaac cctgtatgac gagctcggca tggagaccga
    13141 cccctatcag cgcttcggcg ggctcgacga cgagatgctg cgcgcgatgc ggctggtggt
    13201 cgataccggc atccattcca agggctggac ccgcgaccag gcgatccaat acatgctcgc
    13261 caacagctcg atggggaaga ccgacgccac cgccgaggtc gagcgctaca tcgccatccc
    13321 cagccaggcg ctcgcctata aggtcggcca gctcaagatc cttgaactgc gcgccagggc
    13381 ggagcaggcg ctgggcaacc gcttcgacct caagcgcttc cacgagcagg tgctgatgac
    13441 cggcgctttg cctttgtcgg tgctcgagca gaagatcgac cgctggatcg cctcgcaagc
    13501 ctgacggaaa ccctccccgt gcccagcatg gggaggaatg ggaaacatcg atgaaacacc
    13561 tgccccttct cctcgccgcc gccgccctcg ccggctgcgt cgaaacgcct ccggcccgca
    13621 ccgccgccac tctgcccgcc gccgccgccg aaaatgccgg cgaggcgctc gccgccttgt
    13681 tcgccgccag cgacgaagcc agcctcgagc gcaacccgat ccaggcgctg ttccgcggcg
    13741 acctgcgtta tgccggccgg ctcggccatt tcctctccga cgaatattat gccgccgagc
    13801 gcgccgccgc gacccgcgaa ctcgccgcgc tggcggggat cgaccgggcg gcgctgagcc
    13861 cttcggagcg catctcctac gacgtgttca aatggcagcg cgagctcgac cggcgcggcc
    13921 tcgaaccgga catgctggcg ctgaccgcgg tgcgtccgat cgaccatttc aacggcttcc
    13981 atgcccagtt cgcggagctg agcacgggcg agagcatcgc gcccttcaag acgctccagg
    14041 attatgagag cggcctcacc cggatcgacg attatgtcgt caatctcgac caggcgatcg
    14101 tccgcatgcg cgagggcatg gccgcccgcg tcgtccagcc gcggctggtg atggagaatg
    14161 tcgtcgagca gctcgacgcc atgctggcgg aaggcgtcga gggcagcagc ttcatgaagc
    14221 cggtcgccac gacgccggcc gaaattcccg cggcggacca ggtccggctc aggaccgctt
    14281 accggcagtc ggtcgagacg gaaatccagc ccgcgctgac gcggctgagg acctttatcg
    14341 tcggcgaata tctgcccgcg actcgcgcct cggtcggcct cggcgccatg cccggcggcg
    14401 accggctcta cgcctatctc gtccaggcga gcaccaccac cgacctcacc cccgaccaga
    14461 tccaccggat cgggctcgac gaggtgcggc gcatccaggc cgagatggag gcggtgaagc
    14521 aacaggtcgg cttcaagggg acgctccagc aattcttcga gcacatccgc accgacccga
    14581 agttcaagcc cacgtcgcgc gaggcgctcc agcagggcta tgtcgcgatc ggcaagcggc
    14641 tcgaagcgac cttgccgaag ctgttccgga cgctgccgcg ggcgccgctc gacatccgcc
    14701 cggtgccggc gctgaccgag aagggggcgg cgcgcggctc ctacaatccc ggcacgccgg
    14761 acggctcgcg cccgggcatc ttctacttca acgcctacga ccttccctcg cggaccacgc
    14821 cgggcatgga gacgctctac ctccacgaag gcgcgcccgg ccaccatttc cagatcagcc
    14881 tcgcccagga gaatgaggcg ctgcccaaat tccagcggtt cggcggcaac accgcctatg
    14941 tcgaaggctg gggcctctat gccgagacgc tcggcaagga gctcggcgtc tataccgacc
    15001 cctatcaata tttcggctat ctcgattcgc agctattccg cgccatccgg ctggtggtcg
    15061 acaccggcat tcatgccaag ggctggaccc gcgaccagac catcaaatat atcatggcca
    15121 acagctcgcg cggcctcagc aacgccaccg cggagaccga gcgctacatc gccaatcccg
    15181 gccaggcgct cgcctacaag atcggccagc ttaagatcag cgagctgcgc acccgcgccg
    15241 aacgggcgct cgggccgcgc ttcgacctcg ccgagttcca cgagcaggtg ctgacgtccg
    15301 gcgcgctccc gctcgccgtg ctcgaacgca agatcgacgc ctggatcgcc agcaagccgg
    15361 gctgaggccg gcgagacgtc gaagcgctag ttccgcaccg tcaaccgttc gtcccgagcg
    15421 aagtcgaggg accccgccga gcgaagccgg ggcttccgcg agcgccctga gaggaagaag
    15481 ggagaccccg gatcaagtcc ggggtgacga tccaacggtc ggctctcgat ccttcaatta
    15541 ggcagccgcc caaacggcag caggcccttc gcggcgcggg cggcggactt gccgttgccg
    15601 ccgcgcagcg cctgcagcag ggcgctggcc tgcctttgca tcacccggtc gatcgaggcc
    15661 gtcgcccgcc ccttgaccgc cgcttcggcg agcgcgcgcg cccggccgat gtcgccggga
    15721 tcgagcaaga tccggttgag gctggcatac ccggggaaga aggcgcggcc gccgcccagc
    15781 gccaccgcgc ggtcgagcgc ctggaggcca tgcgacttgc gcgcgccgag cgcggttcgc
    15841 gccactaaag gcccaagctc gatcaccgcg ccgagatgcc agccggccag cgccatctgc
    15901 gcttcggcgt cgcgcggatt ggcggcgacc agggcctcaa aggcgcggcg ggcggcgagc
    15961 acgtcggcgc ggctgcgctt gagcttgccg cgatagccga tcgccaccgc ccgctgcagc
    16021 tgcgcttcgc gattcttggg gttgcgcgcg agctccgcgt cggcgccttt gagcgcggcg
    16081 tcgatgcggg cgagcgcggt cggcttgtcg cgggcggaga aagcggcgct gaccagcagt
    16141 tcgcgcgggc tttgggccgc ggccgggctc gagaccgccg ccaccaatgc gaccgccaca
    16201 taacccaatt cacgcatgcc aacaccgccc atcattgttc gttatacccg taactagctg
    16261 aaaaggaacg gcgcgtgaac ggtttcgacc ggccatcgct ttgtcttcat cagcaccggg
    16321 gttcggcgca gcccagcccg ggtcaggcgg ttcagccggc ttcctcagca agtgcctcgg
    16381 cgattagccg gcggctattg tcgactccgt aaagggcgat gaagctgccc atgcgcgggc
    16441 cctgctcgct gcccagcaag gtctcgtaca gcgccttgaa ccagtcgcgc aggcgctcct
    16501 tgccataatg ttgcttgccg atctcataga cgtggttctg gaggatcacg gccgggtcct
    16561 cgtccgggcc gccggccgtg tcgccctggg cgagcagcgc gtccagctcg cgcagcgcct
    16621 cggcttcctc cggcgtcggc gcccgccgcc tcagcgcggg ggcgacgaac cggctgccat
    16681 attcgacggc gtagccgacc aggcgatcga gctccggact cgcctccgcg gtcagcgcgg
    16741 gattgtagcg gttgaggaag ctccagatcg tctcgcggtc gccgacgccg ggcaggccgg
    16801 cgacgttgag cagcaaagcg aagctgatcg gcagccgcgt ctccggaacc tcgcccgcat
    16861 ggacgtggtg gacggggttg ccgagcctct tgtcccacgg ctggtcgggg taagcggcga
    16921 ggatctggta atattcgtcc accgccttgg ggatgatccc gagcgagagc tgcttggcct
    16981 ttttcggctc gcggtagatg tagaagctga ggctctcgcg gggggcgtag gtcagccact
    17041 cgtcgatggt gacgccgttg cccttggtct tggagatctt ctcgccattc tcgtcgagga
    17101 acagctcgta attgaagcct tcgggcgggc gggcgcccag gatccgcgcg atcctggagc
    17161 tctggatgaa gccgtcgata tggtccttgc cggacatctc gtaatcgacg ccgagtgcga
    17221 cccagcgcat cgcccaatcg accttccact ggagcttggc gccgcccgcg aggattgact
    17281 gttcgacccg ctcgccgctc gctggatcgg tatagcgcac gctcccggcg ttgggatcca
    17341 cgacttcgat cggcacttgc aacaccttcc cagtgaccgg gctgagcgga agcacggggg
    17401 aataggtctg ccgccgttcc tcgcgcaggg tgggcagcat cacgtccatg atcgcagtgt
    17461 aattgcgaag caccgacttc agcgtctcgt cgaaccggcc ggaactgtag cagtgcgatg
    17521 aggaaacgaa ctcatagtcg aagccgaagc ggtcgaggaa ctggcgcagc agattgttat
    17581 tgtggtcggc atagctccgg tggccgcagc cgaacggatc cggcacccgg ctcagcggct
    17641 gatcgagatg ctcgtgcatc atgtcctggt gcggaacgcc cggcggcacc ttgcgcatcc
    17701 cgtccatgtc gtcggaaaag gcgatcagcc ggctcggcca gtccgccatt tcggtgagcg
    17761 cgtgccgcac catcagggtc cgcgccactt cctgaaaggt gccgagatgc ggcagccccg
    17821 acgggccata gccggtctcg aacaggatcg gccgatcgcc cggcccgtca gggtagcgct
    17881 tcaggatctt gcgcgcttcc tcatagggcc aggccttggc gaccatggcc gcatcgcgaa
    17941 gctcttgagg taccttcaac gttgtccttt cgttccgtcc gagccatgta gggcggatgg
    18001 cggcttcttt gccttatccc gcgcttcacg caacccatgg cggcgtctgg ctggcggcgc
    18061 cggacggcgc cgtgacggcg ctcggccggg gcgaggccat cgcgcgcgcg gccgagacgc
    18121 cgctgatcat gctgaacgcg cccttggtgg gccagcggct cggctatccc gacctttccg
    18181 ggctcgacct gctcgaactg ttcgccttcg tcttcccggc ccggttcacg gtgccgacgc
    18241 cgaaggggct cgcccaggcg ctcggcctcg cgccacccaa ggacgaggcc ggcgccgcgc
    18301 cgttcctgct gcgggcggcg gccgccttgc tcgaccggat gggggcggcc gactggccgg
    18361 agcgggaagg ggcgtgggcg tcggcgcagg cgctctaccg gctgcgctgg ccgtgggcgc
    18421 ccgccatcgc cgagcggctg aagcggccgg agcgggacga gcgctggctg ttctcgcggc
    18481 tgacggaatg ggaggagacg ccgccgcggc cgcagccgag gccggtcagg ctggtcccgg
    18541 aggaggcggt cgagcggctc gccggcctca ccggccgcgg cgccgagccg cgccagggcc
    18601 agcgcgacta tgccgccgcg gccgccgccg ccttcgcgcc gcgcaatgtc gagggccggc
    18661 cgaacctgct cctggccgag gccggcaccg gcatcggcaa gacgctcggc tatctcgccc
    18721 cggcctcgct atgggcggcc aaggcgggag gcgcggtctg ggtgtcgacc tacacgaagg
    18781 cgctgcagcg ccagctcgac cgcgagggcg cgcgcatctt cccggacgag gcggagcgca
    18841 ggaagaagat cgtcgtccgc aagggccgcg aaaattatct ttgcttgttg aacctcgaag
    18901 acgcgctcca aggcggcttc gccggccgcg ccgcgatcct cgctcaattg gtcgcgcgct
    18961 gggccgctta cacgcgcgac ggcgacatgg tcggcggcga catgccgggc tggctgccga
    19021 ccctgttccg ccgcgccggc gcgaccgcgc tcaccgaccg ccgcggcgaa tgcgtctatg
    19081 ccggctgccc gcattaccgc aaatgcttca tcgaacgctc ggcccgcgcc agccaggacg
    19141 ccgacatcgt catcgccaac cacgcgctcg tcatggtcaa cgccgcgcgc ggccgcgaga
    19201 tgggcaatgc ccccggccgg atcgtgttcg acgaaggcca tcatctgttc gacgccgccg
    19261 attccacctt cgcggccgcg ctcaccggcc aggagacgat cgagctcagg cgctggctga
    19321 tcggccccga aggcaaggcg cgcggccgtc gtcgcggcct ggcggcgcgg ctgaccgacg
    19381 tcgcttccta tgacgaggcc ggcgccgcgg cgctcgaggc ggcggtccag gccgccggcc
    19441 tcctcaccgg cgacggctgg ttgcagcgcg tcgcggagaa catggcgttc gggccgctcg
    19501 aacggctgct cgcggcggtc cgcggcaccg tctatgcccg cgcctccgcc caggatgccg
    19561 gctacgggat cgagaccgag atcgccgacc cggacccggc tctggtcgag gccgccgcgc
    19621 ccgcggtcga ggcgatggag ctcctgctcc gccccttggt cgcgctcggc aaaaggttgg
    19681 aggcggtgat ggaggatgcg cccgactggc tcgacgccca ggcccgggcg cggatcgaag
    19741 gcgcgatcgg cgggctcgcc tggcgggtcc agtcgctgtc ggcctggatc gcgctcgccg
    19801 ccaggatcgg cggccccgcc gaccccgact tcgtcgactg gctggcggtc gagcgcatcg
    19861 acggccgcga atatgatatc ggcatccacc gccgctggct cgatccgacc cggccgctcg
    19921 ccgagaccgt gctgaagccc gcgcatggaa tcatcgtcac ctcggccacg cttcgcggcg
    19981 ccggcgactg ggcggcggct gaggcccgca ccggcgccgc ccatctcgat cacccggccc
    20041 agcgtttcag cgccgacagc ccgttcgatt atgccgccaa tagcgaggtg ctgatcgtca
    20101 ccgacctgaa gcgcggcgac ctcgcgcagc ttgccggcgc ctatgcgcgg ctgatcgagg
    20161 cggcgggcgg cggcacgctc gggctgttca ccgcgatcca gcgcctgaag gcggtccacg
    20221 cccgcatcgc cgaccggctc gcccgcgccg gccttccgct ttacgcgcag catgtcgatc
    20281 cgatcgacac gggcacgctt gtggacatct ttcgcgacga tccgcgcgcc tccctgctcg
    20341 gcaccgacgc gctgcgcgac ggcgtcgacg tgcccggcca ctcgctgcgc ctcgtcgtga
    20401 tggagggcgt gccgtggccg cggccgaccg tgctccacgc cgcccggcgc gcggtcggtg
    20461 gggggaccgc ttatgacgac cgcgtcgtcc gcgcccgcct cgcgcaggcg ttcggccgcc
    20521 tcatccgccg caaggaggat cgcggcgcct tcgtcctgct ctcctccgcc acgccctcgc
    20581 gcctgctcga cgccttcccg cccggagtgg cagtgcggcg ggtgccgctg gcggaagcgc
    20641 tcgaacgggt gcggtccagc ctttcaactg tcacgtcttt ggggcatcag ggcgccgctt
    20701 tcgcacccga agagacccga tgaagacgct gacgctgctg cgccatgcca aatcgagctg
    20761 gaacgacccg gtcgcgcgcg acttcgatcg gccgctcaac acgcgcggcc gcaaggccgc
    20821 ccgcgtggtc ggcgccgaga tgcgggcgct ggggctcgtc ttcgaccggg tgctcgcctc
    20881 gccggcggtg cgggtagtcg agaccttgga agaggtcgcc cgcggctacg gcgccgcgct
    20941 cgcccccgtc tatgacaagc ggatttatct cgcctcgccg ggcgcgttgc tcgatctcat
    21001 ccgcgaggtg gacggcgccg acagcttgct ggtggtcggc cacaatccgg gcatggagca
    21061 gctcgcgctc gctttggccg gcgaggcggc cggcgattgc cggagcgagc tggccctcaa
    21121 atatcccacc gccgccctgg ccgagatccg gcttccggcc gatcgctgga gcgaggtcga
    21181 ggaaggcgcc ggcttcctcg cccgcttcgt ccgcccgcgc gacttggacc cggagctggg
    21241 cccggacgag gacagttagg gctcggcgat cagacggtct ttccgtcatg ccggacttga
    21301 tccggcatct cccttcttcc ttctcgatcg aagttacctg gctcccgtga tccctactcc
    21361 gtcgcgaaca gattctccgg cttcgccatt gccttgatct cgatcgcatt gcccgacggg
    21421 tcgaggaaga acatcgtcgc ctgctcgccc ggctggccgg cgaagcggat gtcgggctcg
    21481 atcacgaact cggttccggc cgcccgcagc cgctcggcca gcgcccgcca ttcggccatg
    21541 ccaagcaccg ccccgaaatg cggcaccggc acgtcgtggc cgtcgaccgg gttgtgatgc
    21601 gcccttggtc tggcctcggg gctgaggtgc gcgacgatct ggtgcccaaa aaagtcgaag
    21661 tcgatccagt gcgccgcgct ccgcccctcg cggcagccga gcagcccccc gtaaaagccc
    21721 cgggcggcat cgaggtcgtg gacgggaaag gcgagatgga atggggggag ctgggtcatg
    21781 gcttagcttc cgatctgatg gcgagcagca cgcagtgaag ggattcgtcg cgcctcgtgg
    21841 tccccgactc acgggcggct tcgatggttt gcatgtcacg cgcccagatt tgctgccact
    21901 cctcggcgcc ggccgcggcc aaggagcggc tcttcgtgta gagctgctcg aacttccgcg
    21961 ccgcctcatt tccgtaacgg ccttgctcag tctccggccg aaaggccacc ccatagccca
    22021 ttgcatagac gacgacgcat cggagataat cgagcggcag ctccgccggc gctatcgaca
    22081 ggcgctccgc ttcctcggac agtgcattct cgatcagcgg tcggcagcgc cgtatctcgg
    22141 cccggaaata agcatcgcca ccgcccgacc gcttagcggc cccctgcact tcggcgagag
    22201 cggccatcgc ccggtcaacg gcactttgtc gtggctccgg gccgcgcgcc agcttttgcc
    22261 gcgtcaaaag agtgaatgcc attgcgcggt ccactaacgc cggatcgccg attgcaggga
    22321 cgaatgccgg cttctgagca tctgcgagcg ccatgaaggc ggcggtacat ctgaggccgg
    22381 catcggcctc ccgcgccggg agcaccggct gcgaggcctg ggcggcgatc aggtacagaa
    22441 acgcgatcac caagccagac ttcctcgatc cggtccgcgg cacggccgcc gctcgcttcc
    22501 gggctcaacc cggagcgaag cccaatacca gccccacaaa cgtttttcct acacccaaag
    22561 cagcagccac gttcgacggc ttcggcgagc cgcaccattg cgccgccgcc ctcgcccggc
    22621 aataggcggc agcgatgaag ttcctcggcc tttccttcct cgccggcgtc gtgagcgcgc
    22681 tcggcttcgc gccgctcggg ctgtggccgc tgacgctcgc cgccttcacc gctttgctgt
    22741 ggctgatcga gaccgcgccg gggctgcgcg ccgcgctcgc ccgcggctac tggttcgggg
    22801 tgggacagtt cgtgctcggc ctgaactgga tcgccaccgc cttcacctac caggcggcga
    22861 tgccggcctg gctcggctgg ctggcggtcg tgctgctctc gctctacctc gccgtctacc
    22921 ccgccatggc ggccgggctg gcctggcgct ggggatcagt cggaaacata agcacccgtc
    22981 attcccgcga aagcgggaat gaggcttctt cttccgcccc gaacccgcct cccgcgctaa
    23041 ccctcatcct ggtcctcgcc gccgcctgga tcgtgaccga atggctgcgc gcgacgatgt
    23101 tcaccggctt cgcctggaac ccggtcggcg tggcgctgct gcccacgccc gcatcgcggc
    23161 tggcggcatg ggtcggcact tacgggctct cgggcgtcgc aatcctgctc gccggcgtgc
    23221 tgctgctcct cgcccgcagg gcgtttcgcc cggccggcct ccttctcgcc gcggtcgccg
    23281 tgccaagcgc cgctttgctg ctcctgggcg gcgctccggc cggcgccggt ccgcctatcc
    23341 gcatcgtcca gcccaatatc ggccaggagg agagatggcg ggaggaggtg atcgaggaga
    23401 atttcgccaa gctcgagcgc ctgtccaagt cggccgcgcg cgggccgcgg ctgctgctct
    23461 ggcccgaatc ggccgtggtc gagccgctgg aggaggaacg cgccgaccct tatcggcagg
    23521 agcgggtgct gcaggtgcgc gagcggcttg gccagatgct cggacccgag gacctgctcc
    23581 tcgccggcgg cgtcgccttc cgctccaccg acggcgcgaa cctcaccagc gccgccaaca
    23641 gcgtctacgc cgtggacacg gccgggcggc tgatcggccg ctacgacaag gcgcatctcg
    23701 tgccctatgg cgaatatctg ccgatgcggc cgatcctctc cgcgatcggc ctgtcccggc
    23761 tcgccccggg cgatgtcgat ttcgattccg gccccggccc gcgcaccctg gacctgcccg
    23821 gcttcggcaa ggtcggcctc caggtctgct acgagatcat cttctcgggc gaggtggtgg
    23881 aggacggcga ccgcccgagc ttcctgttca acccctcgaa cgatgcctgg ttcggcgcct
    23941 ggggcccgcc ccagcatctc gcccaggcgc ggctgcgggc gctcgaggaa gggctgccgg
    24001 tgctgcgctc gaccccgacc ggcatttcgg cggtgatcga cgccgagggc aggctgctcc
    24061 actcgattcc ctggcgcacc gccggggtga tcgatgcccg tctgccggcc gccaaagcgc
    24121 cgaccttgtt tgcccgggcg ggcaatatcc tccccttcct gttcgcgctg gcgctggccg
    24181 cgatcgcgat tgcggtgcgc cgtaaacatg tttaggggca catataacga tatctttata
    24241 tccgtaactc tccccatcat cagcaagaag gccggctttc ccgatgcgca gcaactatct
    24301 cttcacgtcc gaatcggtgt ccgagggcca tccggacaag gtcgcggacc agatctcgga
    24361 tgccgtggtc gatctgatgc tcagcaagga cccggaagcc cgggtcgcct gcgagaccat
    24421 ggtgacgacg cagcgcatcc tgctggcggg cgaggtcagg tgccggggcg tgttcgacct
    24481 ggagagcttt cccgataccg aaggctgggc tccgggatcg cgcgacgaga tcgagcaggc
    24541 cgtgcggcgc accgtcgaga agatcggcta cgagcaggac ggctttcact ggcagaccgc
    24601 ctatttctcg aaccatctcc atggccagtc gcagcacatc gcccagggcg tcgatgaaag
    24661 cggcaacaag gacgagggcg cgggcgacca gggcatcatg ttcggctacg ccaccgacga
    24721 gacgcccgac ctgcttcccg ccaccctcta ttattcccac cgcatcctcg agcgtatggc
    24781 ttcggaccgc cacaacaagg tggtcgattt cctcgagccc gatgccaaga gccaggtgac
    24841 gcttcaatat gagaatggcg ttcccgtccg cgccgccgcg ctggtcgtct cgacccagca
    24901 ttcgccggcg ctctccaacg acgccggcca ggccaggctg cgcgaatatg tgaaaggcgt
    24961 gttcgccgac gtgctgcccg aaggatggct gccggccgac gaggcgatcc acgtcaatcc
    25021 gaccggccgc ttcgagattg gcggccccga cggcgatgcc ggcctcaccg gccgcaagat
    25081 catcgtcgac acctatggcg gcgcggcccc gcatggcggc ggggcctttt cgggcaagga
    25141 cccgaccaag gtcgaccgtt cggccgccta tgtcagccgc tatcttgcca agaacgtcgt
    25201 cgccgccggc ctcgccaagc gctgcaccat ccagctttcc tacgcgatcg gcgtggccga
    25261 gccgctgtcg ctctacgtcg acctccacgg caccgaggcc aacggcacta ccgccgcccg
    25321 actcgagcaa gtgctgccga cgctggtgcg gctgacgccc aagggcatcc gcacccatct
    25381 cggcctcaac aagccgatct acggcgccac cgcggcctac ggccatttcg gccgcaaggc
    25441 ggagggcgac ttcttcacct gggagaagac cgacctcgtc gaccggctgc gggcggccat
    25501 ctgatcgatc gccgaggtga aggggccggc gccagccggc gccgccctcc ccacttacgc
    25561 gtccgggaac ggcagccttc gctggcatga cggggtggcg cgggttacca gccggcgcat
    25621 gcccgatccc accaccatcc gccggctcta cggccgccgc tcgggccaca agctgcgcca
    25681 ggggcaggcg gcgctgctgg aggaattgct gccggcgatc agcgtccccg acgcggggcc
    25741 gatcacttcg gcggccctgt tcggcgacga ccggccgctt catttcgaga tcggcttcgg
    25801 ctcgggcgag catctcgcct accgcgccga tctgctcccc gaccatggct tcatcggcgc
    25861 cgagcccttc atcaacggag tcgtcggcgc gcttcagcat gttcgcgaca ggcagctcgc
    25921 caatgtccgg cttcatctcg gcgacgctct cgacatactc gaacggctgc ccgactcctc
    25981 gctccgcttc gtctatctgc tccacccgga cccctggccc aaggcgcggc atgcgaagcg
    26041 gcgcatggtc aatcccggcc cgctcgacct gatcgccgcc aagctccagc cgggcggcga
    26101 gttccgtctc ggcaccgacg acccggtcta ttgccgctgg tcgatgatgg tgatgagccg
    26161 gcgcggcgat ttcgagtggc tggcggagag cgccaaggat ttcctgaccc gtccaggcgg
    26221 ctgggcggag acgcgctacg aagcgaaagc ccgccgcaag gggcatgaag tctggtattt
    26281 ccggtatcgg agggtgtgat gcgcacgatt gttctcggcc ttatgctgct cgccggcgtg
    26341 gaaggcgcgg cgctcgcctg ctcgtgcatc ccgccgggcg cgcccgaaca gtcgcgtggt
    26401 ttcgcccgtg aggcgttgca gggcgtgacc gcgatcgtcg aggcggaggc gattagcgac
    26461 tatcggcccg gcggcccggg cgaggaggtg cgcgttctgc gtaccctgtt cggcagcgct
    26521 ccgccgacga tccggctgga gcggagcgag catgccagca gcgcatcgtg cgacctgctg
    26581 ctggggaaag gcgagcgcaa ggtgctgatg ctgacgccgg ccgagggcgg cacttatcgg
    26641 atgcagagcc tttgctccga ctatctcact tccgaacgct acctgccgat cctgctggag
    26701 gaagcgggct ggacgagcaa ggcaggcgag cgggccgaac gctgcggcgg cggcatcagc
    26761 gcgccggcct gagcctgagc agccggccgc cctcgccgtc atcctcgtcc tcaagcacgt
    26821 agatcgatcc gtccggcccc tgctcgacct cgcggatgcg ggcgctcatg tcccagcgct
    26881 cggcctcgcg ggcgccggtt ccggtcatct gcacgcgcac cagcgcctcg cccgaaaggc
    26941 cgcccaggaa ggcgttgccg cgccaggccg ggaacagcga ccccgaatag atcatcatcc
    27001 ccgccggcga gaggccgttc caggtgatct cgggcgcgtt atattcgggc cgggtgctgt
    27061 ggtccggaat gtccttgccg tcgtaatggc tgccgttgga gacgatcgga tagccgtaat
    27121 tggagccgcg ctcgatccgg ttgaactcgt cgccattctt ggggcccatt tcgctcgacc
    27181 agagctgacc ctgcccgtcg aaggcgaggc cgaggatgtt gcggtggccc gcgctccaca
    27241 gctgggcggt gacgccgccg cggctcgccc agggattgtc ggcggggacc gagccgtcgt
    27301 cgtcgaggcg gacgaccttg ccgagcgtgg cgctcatgtc ctgcgcgggt gtgaatttct
    27361 ggcggtcgcc gttcgaaatg tagagcttgc cgtcgggccc gaaggcgagg cggtggccgt
    27421 aatggccgcg gccctcgacc ttgggatgct ggcgccagat cacctgcagc ccttcgagcc
    27481 ggccggattc gcccgccacc agcttcgcgc ggccgaccac cgccccgcgc gtgtcgccgt
    27541 cgcccgcttc gacccagctc agatagatga ggttgttgtt cgcataatcc gggtggacga
    27601 tgacgtcgcc gaggccgccc tggccgccat aatcgacctt gggcacgcct ttgaccgggc
    27661 tgacgaggcc gccctcgttc cacagcatca gccggccctt tttctcggtg atcaaagcct
    27721 ggccgctatt ggggaggaag gtcatcgccc acggctcgtc gaacctggcg atatcctcga
    27781 ccgcgaacgg gcggctggcc gaagccttgg gcgcgccggc ggtccccgcc tcggtggcgt
    27841 cgcagctcac ggtaagcaaa gcggggaggg cggcgaggag gatggcgcgc atgggtctga
    27901 tctccggaaa gaaggtgccg tcgtgcaacg aaccctttgt gagaagcgcg ggtttcgtca
    27961 acatggatca accctcgcac tttcggaccg ccgcttgatc cagaacgccc acagctccgt
    28021 cgccctgctc tcgctcgcca ccgcggcgcc ggacatcgtg ctgccgcagg aggaggccaa
    28081 agcgctcgcc gcgcgcctgt tcgggcggcg caccagcctg ttcgaacggc tggcgccggt
    28141 gttcgacaat gccggcatcg accgccgccg catcgtcaag ccgcccgcct ggtatgaggc
    28201 cgagcatggc tggcgcgagc gcaaccgcgt gtatctggag gcggccgagg cgctgtatgt
    28261 cgaggcagcg caaaaggcgc ttggcctggc cggcgtcgcc gcgcacgagg tggacgggct
    28321 cgtcaccgtc tcgaccaccg gcatcgccac gcccagcctc gaagcgcgcg ccgccggccg
    28381 catgggcttc cgcaaggatg cccaaagggt gccggtgttc gggctcggct gcgccggcgg
    28441 cgtcaccggc ctcggcctcg cggcgcggct ggcggcgggg gagccggggc ggacgatatt
    28501 gtacgtgacg gtcgagctct gctcgacctc gatccggctc gacaaattgt ccaaggccaa
    28561 catcgtcgcc accgctctgt tcggcgacgg cgcggcggcc tgcgtgctgc gggcggggga
    28621 gggcggcgtc ggggcgatcg ggccgggggc ggagctgctc tggcccgaga cgctcggcat
    28681 catgggctgg gacgtcgagg atccgggctt ggcggtgatc ttcgaccgct cgatcccgcc
    28741 cttcgtcgag gagcatatgg cggaggcggt cgcgaccatc gccgccaagg cggggatctc
    28801 gccgcgggat atcagccgct tcgcctgcca tcccggcggc gccaaggtgc ttaccgcgct
    28861 cgagggaagc ctcgggctag cggagggggc gctcgaccat gagcgggcgg tgctccgcga
    28921 atatggcaac atgtccgcgc cgaccgtgct gttcgtgctg gagcggctgc tggcggaggg
    28981 gctgccggag cggacgatgc tgctggcgct ggggcccggc ttcaccgcga gcggcctgct
    29041 gctgaccgcg cgatgacggc ggcgatcgtc atcctcacgc tggtgacgct gcagcggctg
    29101 gcggagttgc tggtcgcgcg gaggaacacg cgcgcattgc tcgccgaggg cgggcacgag
    29161 gtcggggccg gccattatcc gctgatcgtc gcggtgcacg ggctgtggct ggcggcgctc
    29221 tggtggctgg cgccggggcg ggcgatcgta tggccgctga tcggcctgtt tctgctgctc
    29281 caggcggggc ggctgtgggt gctggcgacg ctcggccggc gctggaccac gcggatcatc
    29341 gtcctccccg gcgcgccttt ggtgacgagc gggccgtttc gcttcgtgcg ccaccccaac
    29401 tacctcgtcg ttgcgggcga gattgcggtg ctgccgctcg cctttgggct ctggaagctt
    29461 gccctcctct tctccgtcct caacgccgca atcctcgtca ttcgcatccg cgccgagaat
    29521 ggggcgctgt cggacccgcg ctaagtcgcc ggcggcggcg gggcgggggg agcgtcggcg
    29581 ctcgccagca cttcatgctt gtcctcgtgc aggtagagcc ggcggacgag caccagcacg
    29641 acgatggtga gcggcgtcgc cagcagcacg ccggcgaccc cgaacaatat gccggccgcg
    29701 atgatgccga agatcgtcac cgccgggggg aggtcgacca tgcggttctg gagcaaaggc
    29761 atgacgaggt tgccctgcag ctgctgcacc gcgatcagga cgccgaccgt ccacaggccg
    29821 gtgtcggtgc cctggccgaa gccgaggatc acggcgggaa tgcccgcgac cagcacgccg
    29881 agataaggca cgaactcggc caggccggcg accagcccga tcgcccaggc ggccggtagc
    29941 ccgaccagcc acagcccgaa gccggtggcg aggccgacga acaccatgcc cagcgcctgg
    30001 cccttgagcc agttgcgcag cgacaccgcg accgcgtcga acgtctcgat agcgggcgcc
    30061 tgcgcccgcg cgggaaacag gcggaggagg ccgtgcgagt agagcgtcgg ctgggcggcc
    30121 agatagaggc cgccgatcag cacgatgagc agccccgaca ccgcaccgcc gaccgcggtg
    30181 agcgtggccg tgaccgcgct gacgatcgcc tgcccgctcg gcgccaggcc ctgcgcgcgc
    30241 tgcaggatcg ccgcgccgat gggggtggtt tgcagccgcg cctggaagtc ggcccaggcg
    30301 gcggggaggc gggtggcgag ctcggcgaac tcgccctgca gcgtcgcccc gaaaaaggcg
    30361 aaggcgccgc cgatcaacgc cagcaggccg agcacggtgg cggtgagcgc gacggcgaag
    30421 ggcagccggg tgcgccggcg gatggagttg gtgatggtga gcaggatcag cgagaccaaa
    30481 gcgcaggcga acagcagcag cagcaggtcg accagcaccc agagcaggta ggcggctgtc
    30541 agagtcgcga tcaccaccgc ggtggcgcgg aacacgcggc gcgcgagcgt gtcccgcacg
    30601 tggggcgcgg gcg
//
LOCUS       DASVRK010000317         6475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_171777, whole genome shotgun sequence.
ACCESSION   DASVRK010000317 DASVRK010000000
VERSION     DASVRK010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171777"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VF589_13290"
     CDS             complement(<1..541)
                     /locus_tag="VF589_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HEX8258600.1"
                     /translation="MAILDLANLGTRGFIIQGDAAGDLAGGSVASAGDINGDGYGDVI
                     VGADAGDDGGASAGEAYVIFGKASGFGPVDLGNIGAAGFVIQGDAALDKAGFSVASAG
                     DVNGDGLGDVIVGAPFGDDGGTDAGEAYVIFGKAGGFGTIDLTNLGETAGFVIQGDEA
                     GDNVGHSVASAGDFNHDGID"
     gene            666..2003
                     /gene="gltX"
                     /locus_tag="VF589_13295"
     CDS             666..2003
                     /gene="gltX"
                     /locus_tag="VF589_13295"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX8258601.1"
                     /translation="MTIVTRFAPAPTGRLHVGNIRTALHNWLWARKQSGRFLLRLDDT
                     DRERSSEDHADAIRADLAWLGLAADGEHRQSQRFGYYDAALERLRAAGRVYPAWETAQ
                     ELDLKRKIQLGRGRPPVYDRAALSLSSEEIAKLEAEGRRPHWRFRLDHGGPIEWDDLI
                     RGPQHFDPALLSDPVVRREDGSWLYMLPSVVDDVEMGVTHVVRGEDHVVNTAIQVQMF
                     EALGAAPPAFAHEALLVGSEGKLSKRLGSIGVEAMREAGIEPLALLAMLARIGTSLPI
                     EPVAGLEPLVESFDFAHFGRAPARFDMDELAGLNARIVHQLAYDAVADRLPAGMGEDA
                     WAAIRPNLASVAEAADWWHIVEGPVEHRAAAEDREFLAAAAEAAAGIDWSDSPWQALT
                     ASLKAGTGRSGKKLFLPLRQALTGRDSGPEMALLLVLIGKEQSLARLSGAAGA"
     gene            2116..2790
                     /locus_tag="VF589_13300"
     CDS             2116..2790
                     /locus_tag="VF589_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8258602.1"
                     /translation="MPQGGFLEQKPRSPGSLAIVIALHAAAITALALSKMEMPKAEDL
                     GPLIIKDIPVDPDPPPVPVQKPADPKTAKQKTAVTIVPPRVTTPRNDPIVFSDPGPVD
                     PPVFTSLPPGPTTFPLPVPLPPPPAPKAEVRKEAQIDPRAELQPPYPSSEERAGKEGS
                     VTLRVLIGPDGRVKAAERLRAASEAFWRATERHALRNWRFRPATLGGKPVESSKVMTV
                     HFELRG"
     gene            2850..3167
                     /locus_tag="VF589_13305"
     CDS             2850..3167
                     /locus_tag="VF589_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258603.1"
                     /translation="MPLPRPASPRALWADLRNFTRERSPHQWVAAFFALVMPLVIVVG
                     FYFDAQTNIMPGEQIVYVDSWSGNRTDEEIIAAQKAREEEQKAIAAERQRQFKELEKK
                     FGM"
     gene            3185..4132
                     /gene="ribD"
                     /locus_tag="VF589_13310"
     CDS             3185..4132
                     /gene="ribD"
                     /locus_tag="VF589_13310"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX8258604.1"
                     /translation="MEAAIALGERGRGRTAPNPNVGCIVVRDGMVAGRGWTQPGGRPH
                     AEAMALAEAGAAARGAEIYVTLEPCAHRSERGPACADLLAEARPARVVVALRDPDSRT
                     DGRGLARLAAAGIAVETGLAAEAAERSLAGFLSRIRRGRPYITLKLAMSLDGKIALPS
                     GESRWITGEDARAHVHLERARADMILVGRGTYEADAPRLDVRLPGLEDRSPHRALLTR
                     GGAVEGWTRLGAPEEVHSLEGVNDLLVEGGSGAASAFLAADLVDRMLIYRAPILIGEG
                     KSALGYVGLTALADAHNRWDLQDSRMLGRDRLEVYERTK"
     gene            4145..4747
                     /locus_tag="VF589_13315"
     CDS             4145..4747
                     /locus_tag="VF589_13315"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX8258605.1"
                     /translation="MFTGIITDIGRIEAVEQRGDLRLRIGCGYDVAGVDLGASIACSG
                     VCLTVVDKGADWFAVDVSAETLSRTAPGMWQEGRRLNLERPLKVGDELGGHIVTGHVD
                     GIGEVQGAARIGDSTRVTILAGEEVAPYVAAKGSISLDGASLTVNAVESVSDGVRFEV
                     NIIPHTAAHTTFDEMAPGRTLNIEIDILARYLGRMMELRR"
     gene            4806..6092
                     /gene="ribB"
                     /locus_tag="VF589_13320"
     CDS             4806..6092
                     /gene="ribB"
                     /locus_tag="VF589_13320"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX8258606.1"
                     /translation="MSTILIERLCEMVEAGEMSRAGLARAAGLHANSLRKLGDADWNP
                     TADTLLKLERFLQSGGQGVMASAEQIIDEARNGRMFILVDDDDRENEGDLVIPAQMAT
                     PDAINFMARHGRGLICLALAKSRVEQLGLGLMSRHNGSRHETAFTTSIEARDGVTTGI
                     SAGDRARTIAVAIDASKGREHIVTPGHVFPLVAKDGGVLIRAGHTEAAVDVSRLAGLN
                     PSGVICEILKEDGTMARMDDLVPFAQLHGLKIGTIRDLIAYRLKVDRLVEQTAEARFE
                     SRWGGTWTARTFLNKALGNEQIALVKGKVDPSKPTLVRMHALSLFADAFAEAGCRGGL
                     LEGAMKIIGEEGSGVIVVINRPMPDALSRALNIRSGKAETRDMEELRDYGVGAQILSA
                     LGVQDMVLLTNTHHTPVALGGYGLKIVGERPIPGEG"
     gene            6095..>6475
                     /gene="ribH"
                     /locus_tag="VF589_13325"
     CDS             6095..>6475
                     /gene="ribH"
                     /locus_tag="VF589_13325"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX8258607.1"
                     /translation="MARILIVEARFYEHLNDLLLAGARAAIEAAGHGHEIVTVPGALE
                     VPAAIALAAETGRYDAYVALGVVIRGETYHFEIVANESARGIMALTLDGLAIGNGILT
                     VEEEAQALARARPDDKDKGGEAAKA"
BASE COUNT         1051 a         2292 c         2184 g          948 t
ORIGIN      
        1 cgtcgatccc gtcatggttg aagtcgcccg ccgatgcgac gctgtggccg acattgtcgc
       61 cggcttcgtc gccttggatg acgaagccgg cggtttcgcc gagattggtg agatcgatcg
      121 tcccgaagcc ccccgccttg ccgaagatca cataggcttc gccggcatcc gtcccgccat
      181 cgtctccgaa gggcgcgccg acgatgacat ccccgagccc atcgccgttg acgtccccgg
      241 cggaggcgac gctgaagccg gccttgtcga gtgcggcgtc gccctggatg acgaagccgg
      301 ccgcgccgat attgccgaga tcgaccgggc cgaagccgct cgctttgccg aagatcacat
      361 aggcctcgcc ggcgctggct cccccgtcgt cgccggcatc cgcgccgacg atcacgtcgc
      421 catatccgtc gccgttgata tcgccggccg acgcgacgct gccccccgcg aggtcgcccg
      481 ccgcgtcgcc ctgaatgatg aacccgcgcg tgcccaggtt cgccagatcc aggatcgcca
      541 tgattcttct cccacagatc aagaaacatg cgccttaacg agatcgatgg cgagtccttg
      601 ctggccgggc ggcatctctc gaagccgatt gcttcggcgc cgcactcacc gcattgacgg
      661 cagccatgac aatcgtcacc cgcttcgctc ccgcccccac cggccgcctc catgtcggca
      721 atatccgcac cgcgctgcac aattggctgt gggcgaggaa gcagagcggc cgcttcctgc
      781 tgcggctcga cgataccgac cgcgagcgca gtagcgagga tcatgccgac gcgattcgcg
      841 ccgacctcgc ctggctcggg ctggcggcgg acggcgagca ccggcagtcg cagcggttcg
      901 gctactatga cgcggcgctc gagcggctgc gcgcggcggg gcgggtctat ccggcctggg
      961 aaaccgcgca ggagctcgat ctcaagcgca agatccagct tgggcgcggc cgcccgccgg
     1021 tctacgaccg cgcggcgctc agcctctctt ccgaggagat cgcgaagctc gaggcggagg
     1081 gcaggcggcc gcactggcgc ttcaggctcg atcatggcgg gccgatcgag tgggacgacc
     1141 tcatccgcgg tccccagcat ttcgacccgg ccttgctgtc cgacccggtc gtgcggcggg
     1201 aggacggcag ctggctctac atgctgccga gcgtggtcga cgacgtcgag atgggggtca
     1261 cccatgtcgt tcgcggcgag gaccatgtcg tcaacaccgc catccaggtg cagatgttcg
     1321 aggcgctggg ggcggccccg cccgccttcg cgcatgaggc gttgctggtc ggatcggagg
     1381 gcaagctttc caaacggctg ggctcgattg gagtcgaggc aatgcgcgag gcggggatcg
     1441 agccgctcgc cttgctggcg atgctcgccc ggatcggcac cagcctgcca atcgagccgg
     1501 tcgcggggct ggagccgctg gtcgaaagct tcgacttcgc ccatttcggc cgcgccccgg
     1561 cgcggttcga catggacgag ctggccggcc tcaacgcccg catcgtccat cagcttgcct
     1621 atgacgcggt cgcggaccgg ctgccggccg gcatgggtga ggacgcctgg gcggcgatcc
     1681 ggcccaacct cgccagcgtc gccgaggccg cggactggtg gcacatcgtc gaagggccgg
     1741 tcgagcatcg cgccgccgcg gaggatcgcg aattcctcgc cgccgccgcc gaggccgcgg
     1801 ccgggatcga ctggagcgat tcgccctggc aggcgctgac cgcctcgctg aaggcgggca
     1861 ccggccgctc cggcaagaag ctgttcctgc cgctccgcca ggcgcttacc gggcgcgaca
     1921 gcggccccga aatggcgctg ctgctggttc tgatcggcaa agaacaaagc ctcgcccgcc
     1981 tcagcggcgc cgccggcgcc tgatccgcgc ccagccggcc cgcaggcgcg gcagaaagat
     2041 atagtgtaac atcaagatat gatgtatcat ctcggttaga gctggttgaa ccggccatgc
     2101 caaaggagag acgtcatgcc gcaaggaggt tttctagagc agaaaccgag gagccccggc
     2161 agcctcgcca tcgtcatcgc gctccacgcg gcggcgatca ccgcgctggc gctgtcgaag
     2221 atggagatgc cgaaggccga agatctaggc ccgctcatca tcaaggatat cccggtggac
     2281 cccgatccgc cgcccgtgcc ggtccagaag ccggccgatc ccaagaccgc caagcagaag
     2341 actgccgtca ccattgtgcc gccgagggtg acgacgccga ggaacgaccc gatcgtgttc
     2401 agcgaccccg gtccggtgga ccccccggtc ttcaccagcc ttcctccagg cccgaccacc
     2461 ttcccgcttc cggtgccgct cccgccgccg cccgcgccga aagcagaggt caggaaggag
     2521 gcgcagatcg atccccgcgc cgagctccag ccgccttatc cgtcatccga ggagcgcgcc
     2581 ggcaaggagg gctcggtcac cttgcgcgtg ctgatcggcc ccgacgggcg ggtcaaggcg
     2641 gccgagcggc tgcgcgcggc gagcgaggcc ttctggcgcg ccaccgagcg tcacgcgctc
     2701 cgcaactggc gcttccgccc cgccacgctc ggcggcaagc cggtcgaaag cagcaaggtg
     2761 atgaccgtgc acttcgagct aaggggctga tttgaaagca gggcggcgaa gggctggcgc
     2821 tcgggcggcg ccggccctat ctttcggcaa tgccgctccc tcgccccgct tccccgcgcg
     2881 ccctctgggc cgatcttcgc aacttcacgc gcgagcgcag cccgcaccag tgggttgccg
     2941 ccttcttcgc gctggtgatg ccgctggtca tcgtcgtcgg cttctatttc gacgcgcaga
     3001 ccaacatcat gccgggcgag cagatcgtct atgtcgacag ctggagcggc aaccgcaccg
     3061 acgaggagat catcgccgcg cagaaggcgc gcgaggagga gcagaaggcg atcgccgccg
     3121 agcggcagcg ccagttcaag gagctcgaga agaaattcgg catgtgatgg gggacgcgcg
     3181 ctggatggag gcggcgatcg cgctcggcga gcgcggccgc ggccgcaccg cgcccaaccc
     3241 caatgtcggc tgcatcgtcg tcagggacgg aatggtagcc ggacgcggct ggacccagcc
     3301 gggcggccgt ccccatgccg aggcgatggc gctggccgaa gccggcgcgg ccgcccgcgg
     3361 cgccgaaatc tatgtcacgc tcgaaccgtg cgcgcatcga agcgaacgcg gccccgcctg
     3421 cgccgacctg ctggccgagg cgcgtccggc ccgggtggtg gtcgcgctcc gcgatcccga
     3481 ctcgcgcacc gacgggcgcg gcctggcgcg gctcgccgcc gccggcatcg cggtcgagac
     3541 cgggctcgcg gccgaagccg ccgagcggtc gctggccggc tttctgagcc gcatccgccg
     3601 cggccgccct tatattacgc tcaagctcgc catgtcgctc gacggcaaga tcgcgctccc
     3661 gtcgggcgag agccgctgga tcaccggcga ggacgcccgc gcccatgtcc atctcgagcg
     3721 ggcgcgggcc gacatgatcc tggtcgggcg cggcacctac gaggccgacg cgccgcggct
     3781 cgacgtccgg ctgccgggcc tcgaagaccg ctcgccgcac cgcgcattgc tcacccgcgg
     3841 cggcgccgtc gagggctgga cgcggctcgg cgctcccgag gaagtccatt cattggaagg
     3901 cgtcaacgac ctgctggtcg agggcgggtc cggcgcggct tccgcctttc tcgccgccga
     3961 cctggtcgac cgcatgctca tctaccgcgc gccgatcctg atcggcgagg gcaagtcggc
     4021 gctcggctat gtcggcctca ccgcgctcgc cgacgcccac aatcgctggg atttgcagga
     4081 cagccgcatg cttggcaggg accggctcga agtctacgag cggaccaagt agaagcgggg
     4141 caggatgttc accggaatca tcaccgacat cggccgcatc gaggcggtgg agcagcgcgg
     4201 cgacctcagg ctgcgcatcg gctgcggcta cgacgtggcc ggcgtcgatc tcggcgcctc
     4261 gatcgcctgc tcgggcgtgt gcctgaccgt cgtcgacaag ggcgcggact ggttcgcggt
     4321 cgacgtctcg gccgaaacgc tgagccggac cgcgccggga atgtggcagg aggggcgcag
     4381 gctcaacctc gaacgcccgc tgaaggtcgg cgacgagctc ggcggccata tcgtcaccgg
     4441 ccatgtcgac ggcatcggcg aagtccaggg cgccgcccgg atcggcgatt cgacccgcgt
     4501 caccatcctc gccggcgagg aggtcgcgcc ctatgttgcc gccaagggct cgatcagcct
     4561 cgacggcgcc tcgctcaccg tcaacgcggt cgagagcgtc tcggacggcg tgcgcttcga
     4621 ggtcaacatc atcccgcaca ccgccgccca caccaccttc gatgaaatgg cgccagggcg
     4681 cacgctcaac atcgagatcg acatcctcgc ccgctatctc ggccggatga tggagcttcg
     4741 ccgctaacgc tgtagtgtga tccactactt gccactcaca ccagcatgtg atagcggcgc
     4801 caaaaatgag caccatcctg atcgagcgcc tgtgcgaaat ggtcgaagcg ggcgaaatga
     4861 gccgcgccgg cctcgcccgg gccgcgggcc tccacgccaa ttcgctgcgc aagctcggcg
     4921 atgccgactg gaaccccacc gccgacaccc tgctcaagct cgagcgattc ctgcagagcg
     4981 gcggccaggg cgtgatggcc tcggccgagc agattatcga cgaggcccgc aacgggcgca
     5041 tgttcatcct ggtcgacgac gacgatcgcg agaatgaggg cgatctcgtc atcccggcgc
     5101 agatggcgac gcccgacgcg atcaacttca tggcccggca cggccgcggc ctcatctgcc
     5161 tcgcgctggc caagagccgg gtcgaacagc tggggctcgg gctgatgagc cgccacaatg
     5221 gcagccggca cgagaccgcc ttcaccacct cgatcgaagc gcgcgacggc gtcaccaccg
     5281 gcatctccgc cggcgaccgc gcccgcacca tcgccgtcgc gatcgacgcc tccaagggcc
     5341 gcgagcatat cgtcacgccc ggccacgtct ttccgttggt cgccaaggat ggcggcgtgc
     5401 tgatccgcgc cggccacacc gaagcggcgg tcgacgtgtc gcggctcgcc gggctcaacc
     5461 cgtcgggcgt gatctgcgag atattgaagg aagacggcac catggcgcgg atggacgatc
     5521 tcgtcccgtt cgcgcagttg cacggcctca agatcggcac catccgcgac ctcatcgcct
     5581 atcggctgaa ggtggaccgg ctggtcgagc agaccgccga ggcgcggttc gaaagccgct
     5641 ggggcggcac ctggacggcc cgcaccttcc tcaacaaggc gctgggcaac gagcagatcg
     5701 ccctcgttaa gggcaaggtc gatccttcca agccgacttt ggtgcggatg cacgccttga
     5761 gcctgttcgc cgacgccttc gccgaggccg gctgccgcgg cggcctgctc gaaggggcga
     5821 tgaagatcat cggcgaggaa ggctcgggcg tgatcgtcgt catcaaccgg ccgatgcccg
     5881 acgccttgtc gcgggcgctc aacatccgct ccggcaaggc cgagacgcgg gacatggaag
     5941 agctgcggga ctatggcgtc ggcgcgcaaa tcctttcggc gctcggcgtc caggacatgg
     6001 ttctgctcac caacacccac cataccccgg tcgcgctcgg cggctacggg ctgaaaatcg
     6061 tcggcgagcg gccgatccca ggcgagggct gaacatggcg cgaatcctga tcgtcgaagc
     6121 gcgtttctac gagcatttga acgacctgct gctggccggt gcgcgcgcgg cgatcgaggc
     6181 ggcggggcat ggccatgaaa tcgtcaccgt gcccggcgcg ctcgaagtgc cggcggcgat
     6241 cgcgctcgcg gcggagaccg gccgctacga cgcttatgtc gcgctcggcg tggtgatccg
     6301 cggcgagacc tatcatttcg aaatcgtcgc caatgaaagc gcacgcggca tcatggcgct
     6361 gaccctcgac gggctcgcca tcggcaacgg catcctcacc gtcgaggaag aagcccaggc
     6421 gctcgcccgc gcccgccccg acgacaagga caaaggcggc gaggcggcca aggcg
//
LOCUS       DASVRK010000318         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_173780, whole genome shotgun sequence.
ACCESSION   DASVRK010000318 DASVRK010000000
VERSION     DASVRK010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173780"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VF589_13330"
     CDS             <1..1203
                     /locus_tag="VF589_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8258608.1"
                     /translation="FAQLDAVGNQLSAGLPASLYTMLYGLTVGLALLSLYDLQGTCLA
                     GVPAFGARLTAMLLLWSLVCWTLSEHRTVGWDYLVDMAAAVGLLFLVTALVDSEARLR
                     AAIWTVILAGLVSALIVYGDYLTGERLVSTAPAAVTAQFEDFARSAGGSDQNPTTAAQ
                     MLMVSAGLLLGLVVRHGRYRIPAAALLLVCLVALGIMSARSAVLGIAMMVALILYSLR
                     SRRGSALAAAAVLGLIAAAVLLAPPVLVERFAVLGDWSGDPTLFRRLSYLRAGADLFA
                     GSPIWGVGPGNFPLYYSGAEYRYLPGRDPVPRELHNTFADVGVELGLVGFLLFIVLVV
                     VALRSARRASLHEDRLGWTGFATWIALAGLLVACLFMPHKDMRYLWIMLGLALQCGRL
                     AARREAAS"
     gene            1200..2414
                     /locus_tag="VF589_13335"
     CDS             1200..2414
                     /locus_tag="VF589_13335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8258609.1"
                     /translation="MKLLVIVTEYPKATETFIYRDLLKFHELGAEIELHHLAPFRKHQ
                     KLHAFAAPTREWARYTHFAGLEAIVALFALLLTRPLALGRAVGQLIAGYRSHPRLCLK
                     SLALVPKAAAMARRARRQGMDHVHAEFAGHPATAAWLMHRFGGPAYSVSCRAHDIFRT
                     QAMLGPKLGEASTVRTVSEFGRAFLRERLPSLAAREIAVIHSSVDLARIAPAEAAPSD
                     DPFRILFVGALEPKKGVEYLLEALAAAGPRLGEWRCDIVGDGPSRGRLEAQAAGLGLT
                     PHVRFLGALDFDAVSEAYRRAGLCVAPSIIGPGGRQEGIPNVMIEALAFQRPAIATAI
                     SGIPELIRDGDTGLLVPQADAAALAEAILRVRGDPEAAEAMARRGRAFVEAHFDLAAN
                     AARQMALFSKAV"
     gene            2451..>2699
                     /locus_tag="VF589_13340"
     CDS             2451..>2699
                     /locus_tag="VF589_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258610.1"
                     /translation="MLASLDQRALGIVERCLDLEGAARARCIAKACAGSPELLAKVER
                     MLAMDETRFKLLPTEALTTPEAPPDPVPERIGAFKVTGT"
BASE COUNT          326 a          990 c          921 g          462 t
ORIGIN      
        1 ttcgcgcagc tcgacgcggt cggcaaccaa ttgtcggccg ggctgcccgc ctcgctctac
       61 accatgcttt acggcctcac cgtcggcctc gccttgctgt cgctctacga tcttcagggc
      121 acctgcctcg ccggcgtgcc cgccttcggg gcccggctga cggcgatgct gctgctctgg
      181 tccctggtct gctggacgct ctcggaacat cgcaccgtgg gctgggacta tctggtcgac
      241 atggcggcgg cggtggggct gctgttcctg gtgacggcat tggtcgacag tgaggcgcgg
      301 ctgcgcgccg cgatctggac ggtcatcctg gcagggctgg tctcggcgct gatcgtctat
      361 ggcgattatt tgacgggcga gcggctggtt tccaccgcgc cggcggcggt gacggcgcaa
      421 ttcgaggatt tcgcccgctc cgccgggggc agcgaccaga acccgaccac ggcggcgcag
      481 atgctgatgg tgagcgcggg cttgctgctc gggctggtcg tccgccacgg ccgctaccgg
      541 atcccggcgg cggcgctgct gctggtctgc ctggtcgcgc tcggcatcat gtcggcgcga
      601 agcgcggtgc tcggcatcgc gatgatggtg gcgctgatcc tttactcgtt gcgcagccgc
      661 cgcggctcgg cgctcgccgc cgcggccgtg ctcggcctca tcgccgccgc cgtcctgctc
      721 gcgccgccgg tgctggtcga gcgtttcgcc gtgctcggcg actggtcggg cgacccgacc
      781 ctgttccgcc gcctgtccta cctgcgcgcc ggcgccgatc tgttcgccgg ctcgccgatc
      841 tggggcgtcg gccccggcaa cttcccgctt tattatagcg gcgccgaata tcgctacctg
      901 ccgggccggg acccggtgcc gcgcgagctc cacaacacct tcgccgacgt cggcgtggag
      961 ctggggctgg tggggttcct gctgttcatc gtcctggtcg tggtcgcgct ccgctccgcc
     1021 cgccgcgcct cgcttcacga ggaccggctc ggttggaccg gcttcgcgac ctggatcgcg
     1081 ctcgccggcc tgctcgtcgc ctgcctgttc atgccgcaca aggacatgcg ctatctctgg
     1141 atcatgctcg gcctcgcgct ccaatgcggg cgcctggccg cgcggcggga agcggcgtcg
     1201 tgaagctgct cgtcatcgtc accgaatatc ccaaggcgac cgaaaccttc atctaccgcg
     1261 acctcctcaa gttccacgaa ctgggcgcgg agatcgagct gcatcacctc gcgccgttcc
     1321 ggaagcacca gaagctccac gccttcgccg cgccgacgcg ggagtgggcg cgctacacgc
     1381 actttgccgg cctcgaagca atcgtcgcgc ttttcgccct gctgctgacc cgcccgctag
     1441 cgctgggccg agcggtcggc cagttgatcg ccggctatcg cagccatccc cgcttgtgcc
     1501 tgaagtcgct cgctttggtg cccaaggcgg cggccatggc gcggcgggcg cggcggcagg
     1561 gcatggatca cgtccatgcc gaattcgccg gccacccggc gaccgcggcc tggctgatgc
     1621 accgcttcgg cggcccggct tactcggtga gctgccgcgc ccacgacatc ttccgcaccc
     1681 aggcgatgct gggtcccaag ctcggcgaag ccagcaccgt gcgcaccgtc agcgagttcg
     1741 gccgcgcctt cctgcgcgag cggctgcctt cgctggcggc gcgcgagata gcggtgatcc
     1801 attcgagcgt ggatttggcg aggatcgcgc ccgccgaagc ggccccttcc gacgatccgt
     1861 tccggatcct gttcgtcggc gcgctcgagc ccaagaaggg cgtcgaatat ctgctcgaag
     1921 cgctcgccgc ggccggccca cggctcggcg agtggcgctg cgacatcgtc ggcgacggcc
     1981 ccagccgcgg ccggctcgaa gcgcaagccg ccggcctcgg cctgacgccg cacgtccggt
     2041 ttctcggcgc gctggatttc gatgcggtga gcgaggccta tcgccgcgcc ggcctgtgcg
     2101 tcgcgcccag catcatcggg cccggcggcc gccaggaggg cattcccaac gtcatgatcg
     2161 aggcgctcgc ctttcagcgg cccgccatcg ccacagccat ttccggcatt cccgagctga
     2221 tccgcgacgg cgataccggc ctgctcgtgc cccaggccga tgcggccgcg ctcgctgagg
     2281 ccatcctgcg ggtgcgcggc gaccctgaag cggccgaggc catggcccgg cgcggccgtg
     2341 ccttcgtcga ggcgcatttc gaccttgccg ccaacgccgc ccgccagatg gcgctctttt
     2401 ccaaagcggt gtaggctcgc cgccggacat cgcgaccggc ggagaggggc atgctggcga
     2461 gcctggacca gcgggcattg ggcatagtcg agcgatgtct cgacctcgaa ggcgcggcgc
     2521 gcgcgcgctg catcgccaag gcctgcgccg gctcgcccga gctgctcgcc aaggtcgagc
     2581 gcatgctcgc catggacgag acccgcttca agctgctccc gaccgaggcg ctgaccactc
     2641 cggaggcgcc gcccgacccg gtgccggagc ggatcggtgc cttcaaggtc accggcacg
//
LOCUS       DASVRK010000319         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_616686, whole genome shotgun sequence.
ACCESSION   DASVRK010000319 DASVRK010000000
VERSION     DASVRK010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616686"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1809
                     /locus_tag="VF589_13345"
     CDS             <1..>1809
                     /locus_tag="VF589_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8258611.1"
                     /translation="GRGAVSVLVLGGDAVGELDLKAFRAAIERQPAWSDLPVLLIIDR
                     EEARRIAPILDELGQVRLVERPIDPFILERSAVAALRSRHRQSGARDALVQLEEAQAR
                     YRALSETLEQRVGDRTKQLEAAYERLVCEAEERWTAEERLRESEELYRYTVELSQQLV
                     WTADAEGIIRTVSRRFELITGFSEDVPPHEGWMRSVHPDDLPGVLEYWRSALVEGRLA
                     SMDFRMRQADGEYRMFRARAAPRRDETGRIVRWYGTTEDIEEQTRADAALRAAEERYR
                     LAAYATNDAIWDLDVASNVTEWTASKTGFFGYPEEGDTSSGEWWSARVHPEDKDRVTA
                     SLAGAIDGGLSHWAECYRFRRANGEYAEVYDQGYIVRDEHGRARRAVGAMADVTDRHR
                     AEAELRRIQSELIHVSRLSAMGAMASTLAHEINQPLTAVSNYVSGSRRLLREVDAPAI
                     EEIADALEAAEAAAQRAGQIVRRLRELVARGNVQVQAEDLPSLIGDACAIALVDAHLL
                     GISHRVRIGVGAQWVYADRIQIQQVLINLLRNAVQAMAGRPQRTIAIRTVARPGGKVE
                     VSVTDTGAGLADGVADELFSPFHSTKPGGMGIGLSI"
BASE COUNT          289 a          623 c          644 g          253 t
ORIGIN      
        1 cggccgcggc gcggtgtcgg tgctggtgct cggcggcgac gccgtgggcg agctcgacct
       61 caaggctttt cgcgccgcga tcgagcgcca gcctgcctgg tcggaccttc ccgtgctgct
      121 catcatcgac cgcgaggagg cgcgccgcat cgcgccgatc ctcgacgagc tcggccaggt
      181 aaggctggtc gaacggccga tcgacccgtt catcctcgaa cgctcggcgg tggccgccct
      241 gcgctcgcgc caccggcaga gcggggcccg cgacgcactg gtccagctcg aggaggcgca
      301 ggcccgctac cgggcgctca gcgaaactct cgagcaaagg gtgggcgacc gcaccaagca
      361 gctcgaagcc gcctatgagc ggctggtctg cgaggccgag gagcgctgga ccgccgagga
      421 acggctgcgc gagagcgagg agctttaccg ctataccgtc gaactgagcc agcagctggt
      481 gtggaccgcc gatgcggagg gcatcatccg caccgtcagc cggcgcttcg agctgatcac
      541 cggcttcagc gaggatgtcc ccccgcacga gggctggatg cgctcggtgc atccggacga
      601 tctcccgggc gtactcgaat attggcgctc ggcgctggtc gaagggcggc tcgcctcgat
      661 ggatttccgg atgcggcagg cggacggcga gtaccgcatg ttccgggcgc gggcggcgcc
      721 gcggcgagac gagaccggcc ggatcgtgcg ctggtacggc accaccgagg atatcgagga
      781 gcagacccgc gccgacgccg cgcttcgcgc ggccgaggag cgctaccggc ttgccgctta
      841 tgccaccaac gatgcgatct gggacctcga cgtcgcctcg aacgtcaccg aatggaccgc
      901 ctccaagacc ggcttcttcg gctatcccga ggaaggcgac actagctcgg gcgaatggtg
      961 gagcgcccgg gtgcatcccg aagacaagga cagggtgacg gcgagcctcg ccggcgccat
     1021 cgacggcggc ctcagccatt gggccgaatg ctaccgcttc cggcgcgcga acggcgaata
     1081 tgccgaggtc tacgaccagg gctatatcgt ccgcgacgag cacggccgcg cccgccgcgc
     1141 ggtcggcgcc atggccgacg tcaccgaccg tcaccgcgcc gaggccgagc tgaggcgaat
     1201 ccagtccgag ctcatccacg tctcgcgtct gagcgcgatg ggggcgatgg cctcgacgct
     1261 cgcccacgag atcaatcagc cgctcaccgc ggtcagcaat tatgtcagcg gcagccgccg
     1321 gctgctgcgc gaggtcgatg cgccggcgat tgaggagatt gcggacgcgc tggaggcggc
     1381 cgaggcggcc gcgcagcgtg ccggccagat cgtgcggcgg ctgcgcgagc tggtcgcgcg
     1441 cggcaacgtc caggtgcagg ccgaagacct gcccagcctg atcggcgacg cctgcgccat
     1501 cgctttggtc gatgcgcacc tgctcgggat cagccaccgg gtcaggatcg gcgtcggcgc
     1561 gcaatgggtt tatgccgacc gcatccagat ccagcaggtg ctgatcaacc tcttgaggaa
     1621 cgccgtccag gcgatggccg gccggccgca gcggacgatc gccatccgca ccgtcgcgag
     1681 gcccggcggc aaggtcgagg tcagcgttac cgacaccggc gccggtttgg ccgacggcgt
     1741 cgccgacgaa ttgttctcgc cgttccacag caccaagccc ggcggcatgg gcatcggcct
     1801 gtcgatcag
//
LOCUS       DASVRK010000320         4873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_618659, whole genome shotgun sequence.
ACCESSION   DASVRK010000320 DASVRK010000000
VERSION     DASVRK010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618659"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VF589_13350"
     CDS             complement(<1..434)
                     /locus_tag="VF589_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX8258612.1"
                     /translation="MFTDLTLDRPVAEPTPSASTPPPGVAADWTIPQHWERFTAEDHR
                     VWDLLFARQQEMLGGRAVRAFAEGLDILRLSKPGIPDLDELNERLFARTGWTVVSVPG
                     LVPDDIFFGHLAERRFPAGNFIRSAAQLDYLEEPDVFHDVFG"
     gene            819..2516
                     /locus_tag="VF589_13355"
     CDS             819..2516
                     /locus_tag="VF589_13355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8258613.1"
                     /translation="MPFHDLPPLLGDALSARGYDAPTPVQAAVLQPEAEGRDLIVSAQ
                     TGSGKTVAFGLAMAPQLLAENGAMPFIREPLALVIAPTRELALQVSRELVWLYEKAGA
                     RIATCVGGMDPSKERRALAQGAHVVVGTPGRLRDHLERGALDLSALRVAILDEADEML
                     DMGFREDLEEILDATREGRRTLLFSATIPKPILALAKRYQKDALRISTVGEDRGHGDI
                     SYQAMTVAPADIEHAVVNLLRLHEAESALLFCATRENVRRLHSSLTERGFNVVALSGE
                     HSQSERNQALQALRDGRARVLVATDVAARGIDLPNLSLVVHVELPRDAETLQHRSGRT
                     GRAGKKGTAVLVVPYPRRKRVESVLRGARINAEWIKPPTPEDISRSDHERLLAVLLEP
                     VEIEDEDRALAQRLLEQKTPEEIAAALVRVHRARMPAPEEMVDSGSGQAGPRPEGPRA
                     GFEDTVWFRMDIGRRHNADPRWLLPLLCRRGHITKNEIGAIRIAAGETLFEIPRAAAA
                     RFAAALKRTAGEDGDEGGVRIEPVQGTPREEAKQNRRMGPPPRHKAKPFKPKAQRPR"
     gene            2524..2982
                     /locus_tag="VF589_13360"
     CDS             2524..2982
                     /locus_tag="VF589_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEX8258614.1"
                     /translation="MTGLRILVDADACPVKDEIYKVAFRHEVPVTIVSNSVIRVPAHP
                     LVDRVVVGAGFDAADDWIAERSGPGAVVVTGDILLADRCLKAGAAAIAPNGKPFTTGS
                     IGAAVATRAIMADLRAGMGDAQGGPPPFAKADRSRFLSALDEALVRLKRA"
     gene            3143..3487
                     /locus_tag="VF589_13365"
     CDS             3143..3487
                     /locus_tag="VF589_13365"
                     /inference="COORDINATES: protein motif:HMM:NF045455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258615.1"
                     /translation="MAMYIYQTDGYPVGFRISNFIHDLDGTPLGRIFGTHVHRLDGSY
                     VGELFKDSVVEKPVAMVRTLLPVAAPSCQPSPGASFRRRGLVNYGYPDVFHLLLEGRQ
                     QPYEEPMAIAAE"
     gene            3755..>4873
                     /locus_tag="VF589_13370"
     CDS             3755..>4873
                     /locus_tag="VF589_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258616.1"
                     /translation="MARIRGTNSWDDLDGTRYDDTIEGFAGDDRLWGYEGDDLLIGGS
                     GNDELDGGTGADEMRGGTGSDLYIVDSINDVVVENADEGDFDVVYSWRSSYTLGAHVE
                     DLVALGTANFYGVGNGLDNFIHGYVGDDTLDGAGGADRLFGETGNDTYYIDDAGDRAI
                     ELAGEGYDYVFTTLNSYTLGANLNELIFDGTGNFTGTGNELGNFIGGGDGNDTLFGAD
                     GDDQLDGGAGADRMNGGRGDDLYIVDDRGDRVVETSATGGIDQVESSVSFALGANVED
                     LELTGTNAINGRGNGLGNWIDGNSAANTLSGAAGNDWLRGGGGNDRLFGGTGEDRLEG
                     GSGADRFYFDTPLNRDTNVDDILDFGDGNDTIMLDRDVF"
BASE COUNT          816 a         1647 c         1660 g          750 t
ORIGIN      
        1 tggccgaaca cgtcgtggaa gacgtccggc tcctcgagat agtcgagctg ggcggcggag
       61 cggatgaaat tgccggccgg gaagcggcgc tcggcgagat ggccgaagaa gatgtcgtcc
      121 ggcaccaggc cgggcaccga caccaccgtc cagccggtgc gggcgaacag ccgctcgttg
      181 agctcgtcaa ggtcggggat gccgggcttg gacagccgca ggatgtcgag cccctcggca
      241 aaggcgcgga cggcgcggcc gcccagcatc tcctgctggc gagcgaagag caggtcccag
      301 acccggtgat cttccgccgt gaagcgctcc caatgctggg ggatggtcca gtcggcggcg
      361 acgcccggag gaggcgtcga agccgaagga gtcggttcgg caacgggacg gtcgagggtg
      421 aggtcggtaa acatggtggg gtattcctct cgcaaagaaa aaccccgccc ggagagccgg
      481 acggggtcga aatgagcagg ctgttgtcgt cagcgcgtca gatcggtccc gcccgggcgg
      541 atccgtaata ataataagcg gcggcggcgg acggcatcgt gcgaacggcc ggggaggccg
      601 ttccgagggc ccgcttatgg ggccgaagat gcgcgttgcc agtcatttcc ctgagccttg
      661 tcgcttcgcc ccccgtctgt caacgatggg cacgtctttc accatgcctg cccgcagctt
      721 aacctccgat tgcccgcgcc gccggagatg gcggcgctgt ggaataccgt tgccccgccc
      781 ttgccccggc ggctctgcgt cccacatagg gcagctctat gccattccat gaccttccgc
      841 cgttgctcgg cgacgccctc tccgcgcgcg gctatgacgc gcccactccc gtccaggccg
      901 ccgtgctcca gccggaggcg gaggggaggg acctcatcgt ctccgcccag accggttcgg
      961 gcaagaccgt cgcgttcggc cttgcgatgg cgccgcagct gctcgccgaa aacggcgcca
     1021 tgcccttcat ccgcgagccg ctggcgctgg tgatcgcgcc cacccgcgag cttgcgctcc
     1081 aggtcagccg cgagctggtc tggctgtacg agaaagcggg cgcgcggatc gccacctgcg
     1141 tcggcggcat ggatccgtcc aaggaacgcc gcgcgcttgc ccagggcgcg catgtcgtgg
     1201 tcggcacccc gggccggctg cgcgaccatc tcgagcgcgg cgcgctcgat ctgtccgccc
     1261 tccgcgtcgc aatattggac gaggcggacg agatgctgga catgggcttc cgcgaagatc
     1321 tcgaggagat actcgacgcg acccgggaag gtcgccggac cttgctcttc tcggccacca
     1381 ttcccaagcc gatcctggcc ctcgccaagc gctaccagaa agacgcgctg cgcatctcca
     1441 ccgtcggaga ggatcgcggc catggcgaca tcagctacca ggcgatgacg gtcgccccgg
     1501 ccgacatcga gcatgccgtc gtcaacctgc ttcgcctgca cgaagcggaa tcggctttgc
     1561 tgttctgcgc caccagggaa aatgtacgcc ggctgcattc gagcctgacc gagcgcggct
     1621 tcaacgtcgt cgctctttcg ggggagcata gccagagcga gcgcaaccag gccttgcagg
     1681 cgctccgcga cggccgggcg cgcgtgctgg tcgcgaccga tgtcgccgcc cgcggcatcg
     1741 acctgccgaa tctgtcgctg gtcgtccatg tcgagctgcc gcgcgacgcc gaaacgctgc
     1801 agcaccgctc cggccgcacc ggacgggccg gcaagaaggg cacggccgtg ctcgtcgtgc
     1861 cctatccgcg ccgcaagcgg gtggaatcgg tgcttcgggg cgccaggatc aacgccgaat
     1921 ggatcaagcc gccgaccccg gaggatataa gccgcagcga tcatgagcgt ctgctggccg
     1981 tgttgctgga gccggtcgag atcgaggacg aggatcgcgc gctggcacag cgtctgctcg
     2041 agcagaaaac ccccgaagag atcgccgctg cgctggttcg cgtgcatcgc gcccgtatgc
     2101 cggcgcccga agagatggtg gattccggtt caggccaggc agggccgcgg ccggagggcc
     2161 cgcgcgccgg cttcgaggac acggtctggt tccggatgga catcggccgg cggcacaatg
     2221 cggacccgcg ctggctgctg cccttgcttt gccggcgcgg ccacatcacc aaaaacgaga
     2281 tcggcgcgat ccgcatcgcc gcgggcgaga ccttgttcga aatcccccgc gcggcggcgg
     2341 cgcggttcgc ggccgcgctg aaacgcaccg ccggcgagga cggcgacgaa ggcggcgtta
     2401 ggatcgagcc ggtgcaggga actccgcgcg aggaggcgaa gcagaaccgc cgcatggggc
     2461 cgccgcctcg gcacaaggcc aagccgttca agcccaaggc gcagcggccg cggtgaggcg
     2521 ggcatgaccg gcctccgcat cctggtcgac gccgacgcct gccccgtgaa ggacgagatc
     2581 tacaaggtcg ccttccgcca cgaggtgccg gtgacgatcg tcagcaatag cgtcatccgc
     2641 gtccccgccc acccgctggt cgaccgggtc gtggtcggcg ccggcttcga tgccgccgac
     2701 gactggatcg ccgagcgctc cgggccggga gcggtggtgg tgaccggcga catcctgctc
     2761 gccgaccgct gcctcaaggc gggggcggcc gcgatcgctc cgaacggcaa gccgttcacg
     2821 accggctcga tcggcgccgc cgtcgccacc cgggcgatca tggcggacct cagggccggc
     2881 atgggcgacg cacagggcgg cccgccgcca ttcgccaagg ccgaccgttc gcgcttcctg
     2941 tcggcgctgg acgaggcttt ggtgcggctg aaacgcgcct gattaacggt tcggcgacga
     3001 tatgatgcgg ctcggctcgt gcctgtggat agcttgactc ggctccattg gcgttccccc
     3061 gtgcatgggt taccccatcc gcgttcaagg gcgggcgtta accacggttt cggttagcgg
     3121 agttgagtta aggggcgcat caatggcgat gtatatttac caaaccgacg gttatcccgt
     3181 cggcttccgt atcagcaatt tcatccatga tctcgacggc acgccgctcg gccggatctt
     3241 cggcacgcac gtgcaccggc tcgacggctc ctatgtcggc gagctgttca aggacagcgt
     3301 cgtcgaaaag ccggtggcga tggtgcgcac tctcctgccg gtggcggcac cgtcgtgcca
     3361 gccgagcccc ggcgcgagct tccggcgccg cggcctcgtc aattacggtt atccggacgt
     3421 gttccatctg ctcctcgaag gccggcaaca gccttatgag gagccgatgg cgatcgccgc
     3481 cgaataatcg gcggctggcg cggcggcgtt accccccgcc gccgcgcctt ttcgtccccg
     3541 gcggccggcc ggcccggcta cgcgcacagc gggccgagcg gcccatagcg ggacatcaag
     3601 cacctgattt ttttggctaa gggtgctcgg gatcaacttc cttaatccgg cgttcaactc
     3661 gaccggcgcg aatatcggcc gacccgtcgc ccaggcggcc ggagtcgcgt tttcgaaagc
     3721 cgatcgctcg atcgcacagt aggagttttc tcgaatggcc aggatccggg gcaccaacag
     3781 ctgggacgac cttgacggca cccgctatga cgacaccatc gaaggctttg ccggggacga
     3841 ccggctgtgg ggctatgagg gcgacgatct tctgatcggc ggctcgggca atgacgaact
     3901 cgacggcggc accggcgcgg atgagatgcg cggcggcacc ggcagcgacc tctacatcgt
     3961 ggactcgatc aacgacgtgg tcgtcgagaa cgccgacgag ggcgacttcg acgtcgtcta
     4021 ttcgtggcgc agcagctaca cgctcggcgc ccatgtcgaa gacctggttg cgctcgggac
     4081 cgccaatttc tacggcgtgg gcaacggcct cgacaatttc atccacggct atgtcggcga
     4141 cgacacgctc gacggcgcgg gcggcgccga ccggctgttc ggcgagaccg gcaacgacac
     4201 ttattatatc gacgacgccg gcgaccgggc gatcgagctg gcgggcgagg gctatgatta
     4261 tgtcttcacg accctcaaca gctacacgct cggcgccaac ctcaacgagc tgatcttcga
     4321 cggcaccggc aatttcaccg gcaccggcaa cgaactcggc aatttcatcg gcggcggcga
     4381 cggcaacgac accctgttcg gcgccgacgg cgacgaccag ctcgacggcg gcgccggggc
     4441 cgatcgcatg aacggcggca ggggcgacga cctctacatc gtcgacgatc gcggcgaccg
     4501 ggtggtggag accagcgcga ccggcggcat cgaccaggtc gaaagctcgg tcagcttcgc
     4561 gctgggcgcg aacgtcgagg acctcgagct caccggcacc aacgcgatca acggccgcgg
     4621 caacgggctt ggcaactgga tcgacggcaa ttcggcggcc aacacgctga gcggcgccgc
     4681 cggcaacgat tggctgcgcg gcggcggcgg caacgaccgc ctgttcggcg gcaccggcga
     4741 ggaccgcctg gaaggcggca gcggcgccga ccgcttctat ttcgacacgc cgctcaatcg
     4801 cgacaccaat gtcgacgaca tcctcgattt cggcgacggc aacgacacga tcatgctcga
     4861 tcgcgacgtt ttc
//
LOCUS       DASVRK010000321         8552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_620268, whole genome shotgun sequence.
ACCESSION   DASVRK010000321 DASVRK010000000
VERSION     DASVRK010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620268"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /gene="trpD"
                     /locus_tag="VF589_13375"
     CDS             complement(<1..637)
                     /gene="trpD"
                     /locus_tag="VF589_13375"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX8258617.1"
                     /translation="MYANTNLAFLPTADAAPAAEPEAALERLYAGLPLSREVAQGLFT
                     ELVEGRLSEPEIAAMLISLRLKGETAEELIGAARALRAADEDFERPDYLFADSCGTGG
                     DGSGSINVSTAVAFVAAAAGLPVAKHGNRSVTSRCGSADVLEQLGAKLDVTPDVSRRA
                     LDETGICFLFAPRYHPGLRHAGPVRRALKVRTIMNMLGPCVNPAEPKVQLLG"
     gene            complement(984..1484)
                     /locus_tag="VF589_13380"
     CDS             complement(984..1484)
                     /locus_tag="VF589_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258618.1"
                     /translation="MLTMKALPPGIGAALLLAGCDAGGGTAPPAAEAPEAAATQPAMK
                     AGDPDQILKVESAQAVFDGFESGDLVWAMLSVPGKEDMRALVALERPGLEHFLAAHAG
                     KPLTLRVETIRTYVEQAGGAIEAQRIADAQTADTTAAAWWQTLDPDQRRAAEAATERL
                     IAPSAE"
     gene            complement(1523..3562)
                     /locus_tag="VF589_13385"
     CDS             complement(1523..3562)
                     /locus_tag="VF589_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8258619.1"
                     /translation="MTRSAVILFLLAASPSQAQEEAEAEIVVTGRGLGEAAGEAVYDV
                     VVIDRERLAGSASNRLEEILREVPGFQLFRRSDARSANPTSQGATLRALGGNASSRAL
                     LILDGVPQTDPFGGWVYWPAYDPRRLGRVRVVRGGGSGAAGPGALAGTIELESAGPED
                     IDGLIGELAYGSRDSLDTYAGLGAALGSGFAAASAHYARGDGFVPIVEADRGPVDRPS
                     PYEQASVAVRGVVPVADAVELQASGLWFSDRRERGTAFTANRTSGADASLRLVGRRWS
                     ALGYVQTRDYYNSFASVGDGRTDVNRVAEQYEVPSTGLGARFEYRPELGPVDLRIGGD
                     WRETAGRTQERYFFQDGVGTRRRIAGGRTRTLGGFAEAAWQSGPLTLTGGGRIDRWRI
                     ADGFLDEREFASETVLTDLAFADRSGWEPTGRAGAAYRMPGGLTVRGAAYLGWRLPTL
                     NELYRPFRAGAHATAANADLAPERLRGIEAGVELRPARALRIGLTAFWNRLEEGIANV
                     TLADGPGTFPGVGFVGAGGQYRQRRNLDAIVSRGIELDGRADIGAWSLSAGYSFADAE
                     VQASGAALPLDGLRPAQTPRHSGAATIAWRGNAARASVTARYVDEQYEDDLNREALPG
                     ALTFDAAAALPLTRSIALEARVENLTNKLVAAAISDGIVERATPRTLWIGLRFGG"
     gene            complement(3630..6329)
                     /locus_tag="VF589_13390"
     CDS             complement(3630..6329)
                     /locus_tag="VF589_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX8258620.1"
                     /translation="MSDFPLILDVTALSERQALEIRSSTLYATAGAFVDCLGDINGDG
                     ASDVFIAAAGGPYIVYGGNGAGSLELQALAPQQGFRISVEQSDGIGPLASAAGDTNGD
                     GFDDFMIGAPGAFEDNYYTPKGGAYVVYGGPSPSGAIALGNLDPSEGFAIVGSGGGLV
                     AGIGIAAAGDVDGDGLDDLLIEEREYIYEEWAHGALVFGSTARTGDVELGSLAPDEGF
                     KIGPFVPFQSQSLDALGDVNGDGLVDFVFGDIEPWTSEAAGPGRAHVVFGKAPGSGNP
                     TLSEGFTIAGEAAGDQFGSMVSGVGDFNGDGLDDILAGAIHHGVGKDRAYLIFGTAAK
                     DPIETAALTPEQGFAIDDPEADGFLRSIGAAGDVNGDGFADLLLRYYYQSYVLFGKAD
                     GFGTVNLADLKADQGFIISGAGADARVSSAGDFNGDGIDDLIVGNPDEDLGARDSGAA
                     WIIYGRAPAAAAVRIGSAGGQVIFGGAFNDTLGGLGGEDLLIGGKGDDRLDGGAGRDA
                     AGYRTSAGGVRVDLALAGAQDTAAAGRDTLVSIENIEGSEHVDLLLGSAASNRLEGQH
                     GDDRLFGRGGRDVLIGGDGADRLLGGLADDRMEGGRGDDWYEVRESLDLIREAGAGGT
                     DTVQAFVTYALAANVEKLVLGGSAGIRGTGNALDNGLVGNSGANLLNGRGGDDRIDGG
                     TGADTMIGGTGEDAFRVDQRGDRIVEFVGEGTDLVLSSVSYTLSQHVEELRLTGTRAL
                     SGTGNAQANRIFGNDAANALQGAGGSDSLSGGLGNDRLSGGAARDALRGGGGDDRFVF
                     DSSLNAGRNVDSILDFVRGDDRIFLDQAVFSGIASEGFLSRAAFREGSVARDASDRIL
                     YDAATGKIFYDEDGTGSATAILFAEVRPGLTLTNLDFSAYGGG"
     gene            complement(6570..7889)
                     /locus_tag="VF589_13395"
     CDS             complement(6570..7889)
                     /locus_tag="VF589_13395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8258621.1"
                     /translation="MSFENLGLSQPVLQALALKEYTVSTPIQEQAIPILLKGRDLLGI
                     AQTGTGKTAAFMLPSIDRLTQSDKRPQPRTCRMLVLAPTRELASQIADSAKAYSRFSH
                     MSVATVFGGTSVSKDRQALSRGVDVLVATPGRLIDLIEQGFLTLRAVEILVLDEADQM
                     LDLGFIHALKQIVKLLPAKRQTLFFSATMPKAIKELADRFLTDPAEVSVAPAATTVER
                     VDQYVTYVQQAEKQALLTMMLRAGFSDKGNMDRVLIFTRTKHGADRVVKLLAGNGIAA
                     NAIHGNKSQPQRERALAEFKGGKTRILIATDIAARGIDVSGVSHVINFELPNVAEQYV
                     HRIGRTARAGAAGVAVAYCAEDERAYLKGIEKLTRQRIEVVPLPENFLAEANRIKSSR
                     TGPALVERPERSPEDRNRVRRPQSRRPNFGAAGAPKTNRSRRRASGR"
     gene            8159..>8552
                     /locus_tag="VF589_13400"
     CDS             8159..>8552
                     /locus_tag="VF589_13400"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8258622.1"
                     /translation="MTARPAERRLAAIMVADVTGYSRMMGLDEERTLERVKALRREIV
                     EPCAARARGRLVKTTGDGFLLEFGSVHDAFRCGRSIQARLRAGDGVGDGEAGPLQLRI
                     GINVGDIIFDGEDVFGDGVNVAARLEPLA"
BASE COUNT         1337 a         2980 c         2903 g         1332 t
ORIGIN      
        1 cgccgagcag ctgcaccttc ggctcggccg ggttcacgca ggggccgagc atgttcatga
       61 tggtgcgcac cttgagcgcc cgccgcaccg gcccggcatg gcggaggccg ggatggtagc
      121 gcggcgcgaa caggaagcag attccggtct cgtcgagcgc gcgccgcgag acgtcgggcg
      181 tgacgtcgag cttggcgccg agctgctcca gcacgtcggc cgagccgcag cgcgaagtga
      241 ccgagcggtt gccgtgcttg gcgaccggca gtcccgccgc cgcggccacg aacgcgaccg
      301 cggtcgagac gttgatcgat cccgatccgt cgccgccggt gccgcaactg tcggcgaaca
      361 gataatcggg ccgctcgaaa tcctcatcgg ccgctcggag cgcgcgggcg gcgccgatca
      421 gttcctcggc ggtctcgccc ttcagccgca gcgagatcag catggcggcg atctcgggct
      481 cggaaagacg gccctcgacc agctctgtga acaggccttg ggcgacctcg cggctcagcg
      541 gcaggccggc atagagccgc tcgagcgcgg cttccggttc ggcggcggga gcggcgtcgg
      601 cggtcggcag gaaggcgaga ttggtgttgg cgtacatcgt gaattccttg gcatcggcgc
      661 gaaaaaaaag cccaccggag cggcgggctt gtcgtgggac ggagcttcag agagtccggt
      721 tcaccatcga tgcaaagcgt gcgccgcggg ggtaatgtgc cgccaccaat gccatgcctg
      781 cgaatcgacc gagcgcgcgg ccgcgtccgc cgaaacggcg gcgggcatgc caaaggggtg
      841 cgcgcaaacg gtcatcccct ccaaactcgc ggaaacggac ttcggggtca atcttttttt
      901 caggctgcac caatgagata gggtttgccg ctgcggcggc ggcctgaacg gcgcggattt
      961 tcgggcggcg agccggaccg tcgttattcg gcggacgggg cgatcagccg ctcggtggcg
     1021 gcttcggcgg cgcggcgctg atcggggtcc agcgtctgcc accaggccgc ggcggtggtg
     1081 tcggcggtct gcgcgtcggc gatgcgctgc gcctcgatgg cgccgccggc ctgctcgaca
     1141 taggtccgga tcgtctcgac ccggagcgtc agcggcttgc cggcatgggc ggcgaggaaa
     1201 tgttcgagcc ccggccgttc gagcgcgacc agcgcccgca tatcctcctt gcccggcacc
     1261 gacagcattg cccacaccag gtcgccggac tcgaagccgt cgaacaccgc ctgcgcgctt
     1321 tcgaccttga ggatctggtc ggggtcgccg gccttcatcg cgggctgggt ggcggcggcc
     1381 tcgggggcct ccgcggccgg cggagccgtc ccgccgccgg cgtcgcagcc agcgagcagc
     1441 agcgccgcgc caatgccggg cgggagcgct ttcatcgtca gcatcgatcc ttagcctcca
     1501 tcgcccggcc tctcccgcct tactagccgc cgaagcgcag cccgatccag agcgtcctgg
     1561 gggtcgcccg ctcgacgatg ccgtccgata tcgctgccgc caccagcttg ttggtgagat
     1621 tctcgacccg cgcctcgagt gcgatcgagc gcgtcagcgg cagcgcggcg gcggcgtcga
     1681 aggtgagcgc gccgggcagc gcctcccggt tgagatcgtc ctcatattgc tcgtcgacat
     1741 agcgggcggt gaccgaggcc cgagccgcgt tgccgcgcca ggcgatggtg gcggcgccgc
     1801 tgtgccgcgg cgtctgcgcc ggacgcaggc cgtcgagcgg aagcgccgcc ccgctcgctt
     1861 gcacctcggc atcggcaaag ctgtagccgg cggacagcga ccaggcgccg atatcggccc
     1921 ggccgtccag ctcgatcccg cgcgagacga tggcgtcgag gttgcggcgc tggcgatact
     1981 ggccgccggc gccgacgaag ccgacaccgg ggaaggtgcc cggcccatcg gccagggtga
     2041 cgttggcgat gccctcttca agccggttcc agaaagcggt gagcccgatc cggagcgctc
     2101 gcgccggccg cagctcgacc cccgcctcga tgccgcgcag gcgctcgggc gcgaggtcgg
     2161 cattggcggc ggtggcgtgg gcgccggcgc ggaacgggcg gtagagctcg ttgagggtcg
     2221 gcagccgcca gccgagatag gcggcgccgc ggacggtgag gccgcccggc atccggtagg
     2281 ccgcgcccgc ccgcccggtc ggctcccagc cggagcggtc ggcaaaggcg aggtcggtga
     2341 ggacggtctc gcttgcgaat tcgcgctcgt cgaggaagcc gtccgcgatc cgccagcggt
     2401 cgatccggcc gccgccggtc agggtgagcg gcccgctttg ccaggcggcc tccgcgaagc
     2461 cgccgagggt gcgggtacgg ccgccggcga tccgccgccg cgtgcccacg ccgtcctgga
     2521 agaaatagcg ctcctgcgtg cgccccgccg tctcgcgcca atcgccgccg atcctgaggt
     2581 cgaccggccc cagctccggg cgatattcga agcgggcgcc gagcccggtc gaggggacct
     2641 catattgctc ggccacacgg ttcacgtcgg tgcggccatc gccgacgctc gcgaagctgt
     2701 tataataatc gcgcgtctgg acgtagccca gcgccgacca gcgccgtccg accagccgca
     2761 gcgacgcgtc cgccccgctc gtccggttgg cggtgaaggc ggtgccgcgc tcgcgccggt
     2821 ccgagaacca caggccgctt gcctgcagct cgaccgcgtc cgccaccggg acgacgccgc
     2881 gcaccgccac gctcgcctgt tcataggggg aggggcggtc gaccgggccg cgatccgcct
     2941 cgacgatggg cacgaagccg tcgccgcggg cataatgggc cgaggcggcg gcaaagccgc
     3001 tccccagcgc ggcgccgaga ccggcatagg tgtcgaggct gtcgcggctg ccataggcta
     3061 gttcgccgat gagcccgtca atatcctccg gcccggcgct ttcgagctcg atcgtgccgg
     3121 caagcgcgcc cggtccggcg gcgccgctgc cgccgccgcg caccacccgc acccgcccca
     3181 gccggcgcgg atcgtaagcg ggccaataga cccagccgcc gaacgggtcg gtctgcggca
     3241 cgccgtcgag gatcagcaag gcgcggctcg aggcgttgcc gccgagcgcg cgcagggtcg
     3301 cgccctggct ggtcggattg gccgagcgcg cgtcggagcg ccggaacaac tggaagcccg
     3361 gcacctccct caatatctcc tcaagccggt tcgaggccga tccggcgagc cgctcgcggt
     3421 cgattacgac gacgtcatag accgcctcgc cggccgcctc gccgagcccg cggccggtga
     3481 cgacgatctc ggcttcggcc tcctcctgcg cttgcgaagg ggaggcggcg aggagaaaga
     3541 gaatgacagc cgagcgagtc atgacgccgc cctatacgcc cgcgtggctt aggccgagcg
     3601 ccttggaatc cgcccctcga cccgcggaat tagccgccac cgtaggcact gaagtccagg
     3661 ttcgtgagcg tcaggccggg ccgcacctcg gcaaacaata tcgccgtcgc cgatccggtg
     3721 ccgtcctcat cgtagaaaat cttgcccgtg gcggcgtcgt agaggatcct gtccgatgca
     3781 tcgcgggcga cggagccttc ccggaacgcc gcccggctga ggaatccctc gctggcgatg
     3841 ccgctgaaca ccgcctgatc gagaaatatc cggtcatcgc ctcggacgaa atcgaggatc
     3901 gaatcgacgt tccggccggc attgagcgag ctatcgaaaa cgaagcggtc gtctccgccg
     3961 ccgcctcgca gcgcatcgcg cgcggcgccc ccgctcagcc tgtcgttgcc gaggcctccg
     4021 ctcagcgaat ccgatccacc ggcaccttga agggcattgg ccgcatcgtt gccgaagatc
     4081 ctgtttgctt gcgcgttgcc cgtccctgaa agagcacgcg tgcccgtcag ccgcagttcc
     4141 tcgacatgct gacttagcgt gtagctgacg gaactaagga cgagatcggt gccttcgccc
     4201 acgaactcga cgatacggtc gcccctttga tcgactcgga aggcgtcctc gcctgtgcca
     4261 ccgatcatcg tgtcggctcc ggttcccccg tcgatgcggt cgtcgccgcc tctgccgttc
     4321 aaaaggttcg cgccggaatt tccaacgaga ccattgtcca gcgcattccc cgtgccgcgg
     4381 atgcccgcgg acccgccaag cacgagcttt tcgacattcg ccgcaagcgc ataggtgacg
     4441 aaagcctgga cggtgtccgt cccgccggcc ccggcttcgc ggatgaggtc gagcgattcc
     4501 cggacctcgt accaatcgtc gccgcggccg ccttccatcc tgtcgtcggc gaggccgccc
     4561 agcagccggt cagcgccgtc gccgccgatc aggacgtccc ggccgccgcg cccgaaaagc
     4621 cgatcgtcgc cgtgctggcc ctcaagccgg ttcgaggctg cggatccgag cagcaagtcg
     4681 acatgttcgg acccttcgat attctcgatg ctgacgagcg tgtcgcgtcc cgccgcggcg
     4741 gtatcctggg ctcccgcaag ggcaagatcg acccgcacgc cgcccgcgct ggtccggtac
     4801 ccggcagcgt cgcgacccgc acccccgtca agccgatcgt cgcctttgcc gccgatcaga
     4861 agatcctcgc cgccgagtcc tcccagcgtg tcgttgaaag caccgccgaa gatgacctgc
     4921 cctccggctg agccgatgcg cactgcggcg gcagcgggcg ccctgccgta gattatccag
     4981 gcagcgcccg aatcgcgcgc gcccaggtct tcgtcgggat taccgacgat cagatcgtcg
     5041 atcccgtcgc cgttgaaatc gcccgccgaa gaaacccgcg cgtcagcacc cgcaccggag
     5101 atgatgaacc cttggtcagc cttgagatcg gcgagattga cggttccgaa gccatcggct
     5161 ttgccgaata gcacgtagct ttgatagtaa tagcgcagca gaagatcggc gaagccatcc
     5221 ccgtttacgt cgccagcggc cccgatcgag cggaggaaac cgtccgcttc gggatcgtcg
     5281 atggcaaacc cttgttccgg cgtgagcgct gccgtttcga tcggatcctt ggccgcggtt
     5341 ccgaagatca gatacgcacg gtctttgcct acaccgtggt ggatcgcacc ggcgaggatg
     5401 tcgtcaaggc cgtcgccatt gaaatcgcca accccgctga ccatcgaccc aaactgatcg
     5461 ccagccgcct ctccggcgat ggtgaatccc tccgacaagg tcgggttgcc cgaccccggc
     5521 gcctttccga aaacgacatg agcacggccc ggccccgccg cctcggacgt ccaaggctca
     5581 atatcgccaa agacgaaatc cacgaggccg tccccgttta cgtcgccgag tgcgtcgagc
     5641 gactgcgatt ggaacggcac gaacggcccg atcttgaagc cttcgtcagg tgcgaggcta
     5701 ccgagctcga catctcctgt ccgggcggtg ctgccgaaga cgagggcgcc gtgggcccac
     5761 tcctcgtaaa tatattccct ctcctcgatc aggagatcgt cgagtccgtc gccgtcaacg
     5821 tctcccgccg ccgcgatccc gatcccagcg acgagccccc cacccgatcc cacgatcgca
     5881 aatccttcac tcggatcgag atttccgagt gcgatcgctc cggacgggga ggggccgccg
     5941 tagacgacgt aggcaccgcc cttaggagta tagtaattgt cctcgaacgc gcccggcgcc
     6001 ccgatcatga agtcgtcgaa tccgtccccg ttggtatcgc ccgcggccga tgcgaggggg
     6061 ccgatgccgt cggactgttc gacggaaatc cgaaagccct gttgcggggc gagcgcctgg
     6121 agttccaacg aaccagcgcc attgccgcca tagacgatgt aaggcccacc cgctgcggcg
     6181 ataaagacat cgcttgcacc gtcgccgtta atgtccccca gacaatcgac gaacgctcct
     6241 gcggttgcat acagagtgct ggatcgaatt tcgagcgcct gtcgctcact cagcgccgtc
     6301 acgtccaaga tcaacggaaa atcagacatt tgtcccccct tcaagtgccg gcatgcatcg
     6361 caggccccgt gccgccgcgc aaggagcgga tcgctgttac ccgccggtcc ccccggccga
     6421 gcggcttacg ccctgatata gcgcagcgcc gattgctcgc aggcatgacg gccgccggtt
     6481 gcgaaaccgc accagcccgg gccgcgcctg atagtccacg agccgtgcgc ggcccccggc
     6541 tgctcaccct cccctagggg atcgggggct taccgcccgg aggcccgcct tctcgaccgg
     6601 ttggtcttgg gcgcgccggc cgcgccgaag ttcgggcgcc ggctctgggg ccgccgcacg
     6661 cggttgcggt cctcggggct ccgctccggc cgctcgacca gggccgggcc ggtccggctc
     6721 gacttgatgc ggttcgcctc ggcgaggaaa ttctccggca gcggcaccac ttcgatgcgc
     6781 tggcgggtga gcttctcgat gcccttgaga taggcccgct catcctcggc gcaataagcg
     6841 accgcgacgc cggccgcgcc cgcgcgcgcg gtccgtccga tgcggtggac atattgctcg
     6901 gcgacgttgg gcagctcgaa attgatcacg tggctgacgc ccgacacgtc gatgccgcgc
     6961 gcggcgatgt cggtcgcgat caggatccgg gtcttgccgc ccttgaactc ggccagcgcc
     7021 cgctcgcgct gcggctggct cttgttgccg tggatcgcgt tggcggcgat gccgttgccg
     7081 gcgagcagct tcaccacccg gtcggcgcca tgcttggtgc gggtgaagat gaggacgcgg
     7141 tccatgttcc ccttgtccga gaagccggcg cgcagcatca tggtgagcag cgcctgcttt
     7201 tccgcctgct ggacgtaggt gacatattga tcgacgcgct cgaccgtggt cgcggccggc
     7261 gccaccgaca cttcggccgg atcggtgagg aagcggtcgg ccagctcctt gatcgccttc
     7321 ggcatggtcg ccgagaagaa caaagtctgg cgcttggccg ggagcagctt cacgatctgc
     7381 ttcagcgcat ggatgaagcc gaggtcgagc atctggtcgg cctcgtcgag caccaggatc
     7441 tcgaccgcgc ggagggtgag gaagccctgc tcgatgaggt cgatcagccg cccgggcgtc
     7501 gccaccagca cgtcgacgcc gcgcgacagc gcctgccggt ccttgctgac cgacgtgccg
     7561 ccgaatacgg tggcgacgct catatggctg aagcggctat aggccttggc gctgtcggcg
     7621 atctggctgg ccagctcgcg cgtcggcgcc agcaccagca tccggcaggt gcgcggctgc
     7681 gggcgcttgt ccgactgggt cagccggtcg atcgacggca gcatgaaagc ggcggtcttg
     7741 ccggtcccgg tctgggcgat gccgagcagg tcgcggccct tgagcaggat cgggatcgcc
     7801 tgctcctgga tcggcgtcga gacggtatat tccttgagcg cgagcgcctg gagaacgggc
     7861 tgcgaaagcc cgagattttc gaaagacata atggactcac ttcatgagcg gcgcccgcgc
     7921 tgccgaaagg gcgcgcggaa acgcggcggg ttttgccgac ccgcgtgaaa agggagagct
     7981 tgaaaacgac gaagcagagc cggtccgagg tcggacgttc acgctgcatg atgcttcgct
     8041 gacgagcgat atgcatgttg cagcgcaaaa agtcaagctc ggaggccgcg gccgtccgcc
     8101 gttgcggaca tccgggcgcg caactaggct gcgcgcgact gcgcggctga gggcaatcat
     8161 gacggcacga cctgcggagc ggcgcctcgc cgcgatcatg gtggcggacg tgaccggcta
     8221 cagccggatg atgggcctcg acgaggagcg gacgctcgaa cgcgtgaagg cgctgcgccg
     8281 cgagatcgtc gagccctgcg ccgcccgggc gcgggggcgg ctggtcaaga cgacgggcga
     8341 cggctttctc ctcgaattcg gcagcgtcca cgacgccttc cggtgcggcc gctccatcca
     8401 ggccaggttg cgcgccggcg atggcgtcgg cgatggagag gccgggccgc tccagctccg
     8461 catcggcatc aatgtcggcg acatcatctt cgacggcgag gatgtgttcg gcgacggcgt
     8521 caacgtggcc gcccggctcg agccgctggc cc
//
LOCUS       DASVRK010000322         5572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_180799, whole genome shotgun sequence.
ACCESSION   DASVRK010000322 DASVRK010000000
VERSION     DASVRK010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180799"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..785)
                     /locus_tag="VF589_13405"
     CDS             complement(51..785)
                     /locus_tag="VF589_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258623.1"
                     /translation="MAGGLAEIYARVLPFARAMPLVAAIPFAAEFAQHIAEIRIGMYA
                     PGGVVTGDEQKLRLAFGAVKVLAIFLALLFALRFWAYRGDAGRAARPTAAMLKGLGLV
                     LLVQAGGELLIIALGAGLARIAGAGPGVTAALTVAPLLLWLLLLTYLLPWFVALLTED
                     RDMTLRRSIDGTEGRLWANFGLLIGSFLPALALHYALGYAAMGRAEPVVWAIMLVDSA
                     LVAALAILLASAYFTLYRRAAGRLAP"
     gene            complement(811..1575)
                     /locus_tag="VF589_13410"
     CDS             complement(811..1575)
                     /locus_tag="VF589_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8258624.1"
                     /translation="MPEGAEAAEAPLRVLIVDDEPLAIERLQLLLARCDGVVLAGTAA
                     DGEAAIRMAEAVGPDLLLLDIAMPGMDGIEVARQLASSSIDPAVIFITAFDNFAVAAF
                     DVAAVDYLMKPVEQERLVRAMGRARDQIAKGRGAGRSHTSSRYVEEFWVPDQNGLVRI
                     AARDVDRITAERDYMRLHVGKRSWLIYRTIAKLEEELDPDLFIRVHRSSILRRDTIVG
                     LSRDGVGHWAARLSDGSEQRIGRSHINEVKRLAGRV"
     gene            complement(1568..2863)
                     /locus_tag="VF589_13415"
     CDS             complement(1568..2863)
                     /locus_tag="VF589_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8258625.1"
                     /translation="MVDRVGDTPLDGDDFGGDEADYGQPPAPLRLFSTNDSGHSANDR
                     SGNSFFLNHALTDDMDMPPRNLIMRRLPDLAIALKSIAGFWVMYMVLITLRSVALMMP
                     DFADAMARRSVATLVGVAITFLIYLAMRPLASASLAKKATLAGFLCLPASVAFAAFNY
                     YVFYILKPIPEEKMDKMWKGMGQLEMAFRSIAEMSLSWYFLFAAWAALYVAMSYATQL
                     RVADRRAAAFAREAQEAQLRALRYQINPHFLFNTLNSLSSLILSQRTDRAERMIMNLS
                     TFFRTTLSADPTADVSLEEEIKLQRLYLDIEQMRFPERLKVEIDIPQDLKTVRVPVLI
                     LQPIVENAIKYGVARSRRAVTLRIQAYEEAGKLHMKVKDDGDAPPPDSGDCGSTGVGL
                     RNVCDRLIARYGVQAGCFHGGDPAGGFTVHVIMPVIRHA"
     gene            complement(2954..3850)
                     /locus_tag="VF589_13420"
     CDS             complement(2954..3850)
                     /locus_tag="VF589_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8258626.1"
                     /translation="MARRYGTDGANIINGTEDDDYLYGYAAGRAAQDTGADRIYGGGG
                     TDYIYGGGGADLLFGDEGEDVIYGGTGNDRIDSGKDNDRVYGENGADTIEAGEGDDLV
                     VGGAGADRIDGQDGLDDLRGGDGADLLYGGAAEDRLLGQDDDDRIWGGDGDDEARGGN
                     GVDLLSGEAGNDFLYGDAANDRLLGGEGTDRLFGGADRDRLDGGVGQDFLSGGAAGDL
                     FVFQGAWDFDTVMDFQNGLDRIDLRQTDLGYGNLTFERVDADSDDVADDLLIKTTEDN
                     YGEIALLNTNQSQITAADFLFG"
     gene            4015..4194
                     /locus_tag="VF589_13425"
     CDS             4015..4194
                     /locus_tag="VF589_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258627.1"
                     /translation="MNRSRVWHDGIDYRTSCGRCDAPLLRGEEGWRPFRSDTDLVVGG
                     LPRKPHPHHRHGDPR"
     gene            complement(4390..4917)
                     /locus_tag="VF589_13430"
     CDS             complement(4390..4917)
                     /locus_tag="VF589_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX8258628.1"
                     /translation="MTAGISVAVAAGSKQASVTVGGAAMYANRNIVENAVNSKDHTTL
                     VAAVKAAGLVETLSGAGPFTVFAPTNAAFAKLPAGTVDTLLQPANLAKLQTVLTYHVV
                     PGRLTAADLMARIKAGGGEARLTTVQGGTLTARMMGKRIMLVDAAGGMSHVTQGNVMQ
                     SNGVIHVTDAVSLPG"
     gene            complement(5050..>5572)
                     /locus_tag="VF589_13435"
     CDS             complement(5050..>5572)
                     /locus_tag="VF589_13435"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX8258629.1"
                     /translation="AALDGEGGRAGNVHQLHRRLTLWRTYSAAISAVAAALLLAIGLD
                     TFGTEEGRAPVQEAARAQPLLVANVAAEDRSAAFVVSYDPSGRSLLVSPAVATPAPGH
                     DHELWLIAGSGAPRSLGLVASTAPHRLPVPAALQPGLRRDATIAISVEPVGGSPTGQP
                     TGPVVASGKLTSV"
BASE COUNT          888 a         1945 c         1874 g          865 t
ORIGIN      
        1 ttcgcctcac gcgacgagcg cgccggatcg agcgaccaac cggcccggct tcagggcgcc
       61 agccgcccgg ccgcgcggcg gtagagggtg aaataggccg aggcgagcag gatcgcgagc
      121 gcggcgacca gtgcggaatc gaccagcatg atcgcccaga ccacgggttc cgcccgcccc
      181 atagcggcgt aaccgagcgc gtagtgaagc gcgagggcgg gaaggaagct gccgatcagc
      241 agcccgaaat tcgcccacag ccggccctcg gtgccgtcga tcgaccgccg caaggtcatg
      301 tcgcgatcct cggtcagcaa ggcgacgaac cagggcagca aataagtgag gagcagcagc
      361 cacagcagca gtggcgcaac cgtcagcgcg gcggtgacgc cgggtcccgc gccggcgatc
      421 cgcgccagcc cggcgcccag agcgatgatg aggagctcgc cgccggcctg gaccagcagc
      481 accagcccga gcccctttag cattgccgcg gtcggccgcg ccgcccggcc ggcgtcgccg
      541 cgataggccc agaagcgcaa ggcgaacagc aaggcgagga agatcgccag caccttcact
      601 gccccgaacg ccagccgcag cttctgctcg tccccggtca caaccccgcc gggcgcatac
      661 atgccgatcc ggatctcggc gatatgctgg gcgaactccg ccgcgaacgg tatcgccgcc
      721 accagcggca tcgcccgcgc gaacggcagc acgcgcgcgt agatttcagc cagtccgcct
      781 gccatctcac cccccttgcg aggcgccgcc tcagacccgt ccggccagcc tcttcacttc
      841 gttgatatgg ctgcggccga tgcgttgctc gctaccgtcg ctgagccgcg ccgcccaatg
      901 gccgacgccg tcgcggctca gcccgacgat cgtgtcgcgg cgcaggatgg acgagcggtg
      961 gacccggatg aacaggtccg ggtcgagctc ctcctcgagc ttggcgatgg tgcggtagat
     1021 gagccagctg cgcttaccga catggagccg catataatcg cgctcggcgg tgatccggtc
     1081 gacgtcgcgg gccgcgatcc gcaccagccc attctggtcg ggcacccaga actcctcgac
     1141 atagcggctg ctggtgtggc tgcgcccggc gccgcggccc ttggcgatct gatcgcgggc
     1201 gcggcccatc gcccgcacca gcctttcctg ctccaccggc ttcatcagat agtcgaccgc
     1261 ggcgacgtcg aacgcggcga ccgcgaaatt gtcgaacgcg gtgatgaaga tgacggccgg
     1321 gtcgatgctg gatgaggcga gctggcgcgc gacctcgatc ccgtccatgc cgggcatggc
     1381 gatgtctagc agcagcaggt ccggcccgac cgcttcggcc atccggatcg ccgcctcgcc
     1441 gtcggcggcg gtgccggcca gcaccacccc atcgcagcgc gccagcaaca actgcaggcg
     1501 ctcgatcgcg agcggctcgt cgtcgacgat cagcaccctg agcggcgcct cagccgcctc
     1561 cgccccctca ggcatggcgg atcaccggca tgatgacatg gacggtgaag ccgccggccg
     1621 ggtcgccgcc gtggaagcag cccgcctgca cgccgtagcg ggcgatcagc cggtcgcaca
     1681 cgttgcgcag ccccaccccc gtgctgccgc aatcgccgct gtcgggcggc ggcgcgtcgc
     1741 cgtcatcctt caccttcatg tgcaatttgc ccgcctcctc ataagcctgg atgcgcaacg
     1801 tcaccgcgcg ccgcgaccgg gcgacgccat atttgatcgc attctcgacg atcggctgca
     1861 ggatcagcac cggcacccgg accgtcttca gatcctgggg aatgtcgatc tcgaccttga
     1921 gccgctccgg gaaccgcatc tgctcgatgt cgaggtagag acgctgcagc ttgatctcct
     1981 cctcgaggct gacgtcggcg gtggggtccg ccgacaaagt cgtccggaag aaggtcgaca
     2041 ggttcatgat catccgctcg gcccggtcgg tccgctgcga caggatcagc gaggagagcg
     2101 agttcaaggt gttgaacagg aagtgcgggt tgatctgata gcgcagcgcc cgcagctgcg
     2161 cctcctgcgc ctcgcgcgcg aacgccgccg cgcggcggtc ggcgacgcgc agctgggtcg
     2221 cgtagctcat cgcgacgtag agcgccgccc aggcggcgaa caggaaatac cagctcagcg
     2281 acatttccgc gatcgagcgg aacgccattt cgagctggcc catccccttc cacatcttgt
     2341 ccatcttctc ttcggggatc ggcttcagga tgtagaagac gtaataattg aaggcggcga
     2401 aggcgaccga ggccggcagg cagaggaagc ccgccaaagt cgccttcttg gcgaggctcg
     2461 ccgaagcgag cggccgcatc gcgaggtaga tgaggaaggt gatggctacg ccgaccaggg
     2521 tcgccaccga gcggcgcgcc atcgcgtccg cgaaatcggg catcatcagc gcgaccgacc
     2581 gcaaggtgat cagcaccatg tacatcaccc agaaaccggc gatcgacttc agcgcgatcg
     2641 caaggtccgg caggcggcgc atgatgaggt tgcggggcgg catatccatg tcgtctgtta
     2701 acgcatggtt cagaaaaaag ctgttccccg accggtcgtt cgcagaatgg ccgctgtcgt
     2761 tggtcgaaaa cagccgcagc ggggccggag gctgcccgta gtccgcctcg tcccctccga
     2821 aatcatcccc gtccagcggc gtgtcgccga ctcggtcgac catcttcaat tccccgctcc
     2881 ccggggcgaa cttatcgcag cttccgcggc gggtacagac cctcgcgctc agggggagcg
     2941 gcagcgtgga gcgctagccg aacaggaaat cggcggcggt gatctgggac tggttcgtgt
     3001 tgaggagggc gatctcgccg taattgtcct cggtcgtctt gatcagcaga tcgtcggcga
     3061 catcgtcgct gtcggcgtcc acccgttcga aggtgaggtt gccgtagccg aggtccgtct
     3121 gccgcaggtc gatccggtcg agcccgttct ggaagtccat cacggtgtcg aagtcccagg
     3181 cgccctggaa gacgaagagg tcgcccgccg ctccgccgga caggaaatcc tggcccacgc
     3241 cgccgtcgag ccggtcgcgg tcggcgccgc cgaacagccg gtcggtgccc tcgccgccga
     3301 gcagcctgtc gttggccgcg tcgccgtaga gaaagtcgtt gccggcctcg ccggagagca
     3361 ggtcgacgcc gttgccgccg cgcgcctcgt cgtcgccgtc gccgccccag atgcggtcgt
     3421 catcatcctg tcccaagagc ctgtcttcgg ccgcgccgcc gtagagcagg tcggctccgt
     3481 caccgccgcg aaggtcgtcg aggccgtcct ggccgtcgat ccgatcggcg ccggcgccgc
     3541 ccaccaccag atcgtcgcct tcgccggcct cgatcgtgtc ggcgccattc tcgccgtaaa
     3601 cgcggtcatt gtccttgccg ctgtcgatcc ggtcgttgcc ggtgccgccg tagatgacgt
     3661 cctcgccctc gtcgccgaac aggagatcgg cgccgccgcc gccatagata tagtcggtcc
     3721 cgccgccgcc atagatgcgg tcggcgccgg tgtcctgggc ggcgcggccc gcggcatagc
     3781 cgtagagata atcgtcatcc tcggtgccgt tgatgatgtt cgcaccgtcg gtgccgtatc
     3841 tgcgcgccat gtctggtctc cattgcgggg atgatccatg tcaacaagcc cgcgcgagcg
     3901 atcgttccta cctgctttgt accacccctt ccgggccgcg ttgcaccgcg aaccgagtgg
     3961 ggccatagtg gcgggatgct tacctcgatg ctctgcccgc tgctcggaca tcgcgtgaac
     4021 cggtcgcgcg tgtggcatga cgggatcgac taccgcacct cctgcgggcg ctgcgacgcg
     4081 cctttgctcc ggggcgagga gggctggcgg ccgttccgca gcgacaccga tctcgtggtc
     4141 ggcggcctgc cccgcaagcc ccacccgcac caccgccacg gcgatccccg ctagcgggcg
     4201 cggatcacgg gatgaacagc aaggtcccga acgccttgcc gatcaggatc agcagcatcc
     4261 attccggcac cacgcggctc aggcgttcga tcatgccgct ctctccatca ttctattggt
     4321 tcggatcgac gccgggatag gacgccgcgg caggggaaac cgcggcgtcc atccgggcga
     4381 ggcgctgcct cagcctggaa gcgacacggc gtccgtgacg tggatgacgc cgttcgactg
     4441 catcacattg ccctgcgtga cgtgggacat gccgccggcc gcatcgacga gcatgatgcg
     4501 cttgcccatc atgcgcgcgg taagcgtacc gccctggacc gtggtcaggc gggcttcgcc
     4561 gccgccggct ttgatccggg ccatgaggtc ggccgcggtg aggcgcccag gcaccacatg
     4621 ataggtcagc accgtctgca gcttggccag gttggccggc tgcaggagcg tgtcgacggt
     4681 gccggccggg agcttggcga aagcggcgtt ggtgggggcg aagaccgtga acgggccggc
     4741 cccggacagc gtctcgacca ggcccgcggc cttgacggcg gcgaccaggg tggtgtggtc
     4801 cttcgaattg acggcgttct cgacgatgtt gcggttggcg tacatcgccg cgccgccgac
     4861 cgtgacggag gcctgcttgc ttccggcggc gacggccacg cttatgccgg cggtcatcgg
     4921 gaggctcatc gcgattatcg ctgctgccag cgcgtttctc ttcttcatgt atcgatcctt
     4981 tttcagcgcg gcccgatcgg gctgctggcc aagctacgga tcgactgcgg ccgcggatgc
     5041 agccggatct cagaccgagg tcaattttcc gctcgcgacc accgggccgg tcggctggcc
     5101 ggtcggggag ccgcctacgg gctcgaccga aatggcgatg gtggcgtcgc gcctgaggcc
     5161 gggctgcaag gccgccggca ccggcaggcg gtgcggcgcg gtcgacgcga ccaggcccaa
     5221 cgagcgcggc gcgcccgacc ccgcgatcag ccacagctca tgatcgtggc caggcgcggg
     5281 ggtggcgacg gcggggctga ccagcaggct gcgcccggaa ggatcgtagc tgacgacgaa
     5341 cgcagcggaa cggtcctccg ccgccacgtt ggcgacgagc agcggctgag cccgcgccgc
     5401 ctcctgcacc ggagccctgc cctcttcagt tccgaacgtg tccaggccga tcgccagcag
     5461 caaggccgcc gccaccgccg agatggccgc ggaataggtc cgccacaggg tcaggcggcg
     5521 gtgcagctga tggacgttgc cggcgcggcc gccctcgccg tcgagcgcgg cg
//
LOCUS       DASVRK010000323         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_182688, whole genome shotgun sequence.
ACCESSION   DASVRK010000323 DASVRK010000000
VERSION     DASVRK010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182688"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VF589_13440"
     CDS             complement(<1..582)
                     /locus_tag="VF589_13440"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX8258630.1"
                     /translation="MKTGLKAGIATAALAAGAAAEAAPVPRAAPVSGRIVAAKGGETA
                     VLVPAPAARRAEVRQDLKAGDVLRTNAAGTLAIIFADRTQVRLGRNTILVVKQVVRGA
                     PSSMQLQRGSLWARSPRGQSRLSVETPSATAAIRGTEYSIVAEEERTSLTVIEGAVDF
                     FNPQGSVRVTEGQSAAARLGQAPTRLIAVNPEAR"
     gene            complement(660..>1796)
                     /locus_tag="VF589_13445"
     CDS             complement(660..>1796)
                     /locus_tag="VF589_13445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8258631.1"
                     /translation="RRERLLEIVDEWRDLGAAAGEANIFFEPMAILPALDFPPPPGLV
                     FFVAWGGDAGGRRQLIGMLPVTRWERKLGFLLPRGIESWDYRLRACGEPLVRAGHERA
                     FWSALFSFVDSQRGAAFLRLPQLHVESASTKALCELAAELERPLHITRRLERAMLRGP
                     VRKEEYLKTLSSKGLANYRRRRKKLEALGTVTSERLQEADGDAAAWFEDFIAMEGRGW
                     KGRSGVAAASTPFMESFTRKLLGEAHAEGRLDLRRLRLDGKTISMIAYVETGRTAISF
                     KITYDEAYAPYSPGVLLHMDHLDHALELDWVDSCATPGHPMYERIWTGKLPLATFMIP
                     ADRPLARIACAAEQAIRGAASRLRKRATGLKHGLRADSSSGKNL"
BASE COUNT          272 a          638 c          625 g          261 t
ORIGIN      
        1 gcgcgcttcg ggattgacgg cgatcagccg ggtcggcgcc tggccgagcc gcgccgccgc
       61 cgactggccc tcggtgaccc gcacgctgcc ctgcgggttg aagaagtcga ccgcgccttc
      121 gatcacggtc agcgaggtgc gctcctcctc ggcgacgatc gaatattcgg tgccgcgaat
      181 ggcggcggtc gccgacggcg tttccaccga cagccgcgac tgtccgcgcg ggctgcgcgc
      241 ccatagcgag ccgcgctgga gctgcatcga ggacggcgct ccgcgcacca cctgcttcac
      301 caccaagatc gtgttgcgcc cgagccggac ctgggtgcgg tcggcgaaga tgatggcgag
      361 cgtgccggcg gcgttggtgc ggagcacgtc gccggccttc agatcctggc ggacttcggc
      421 gcggcgggcg gcgggcgcgg ggaccagcac cgccgtctcg ccgcccttgg cggcgacgat
      481 gcgtcccgag accggggccg cgcgcggcac cggcgccgcc tcagcggccg cgcctgcggc
      541 cagagcggcc gtggcgatgc cggccttcaa acccgttttc atgtcagacc ccttcccccc
      601 ggagagccag gaaaggcagc caataggtag gcgggcgccc acgcaagaaa tattttcgat
      661 taaagattct ttccggacga cgaatccgcc cgcaatccgt gcttcaggcc cgtggctcgc
      721 ttgcgcagcc gcgacgccgc gccccggatc gcctgttcgg ccgcgcaggc gatccgcgcg
      781 agcgggcggt cggctgggat catgaacgtg gcgagcggca gcttgccggt ccagatccgc
      841 tcgtacatcg ggtggcccgg cgtggcgcag gaatcgaccc agtcgagctc gagcgcatgg
      901 tcgagatggt ccatgtgcag cagcacgccc ggcgaatagg gggcataggc ctcgtcatag
      961 gtgatcttga acgagatcgc ggtgcggccg gtctcgacat aagcgatcat gctgatcgtc
     1021 ttgccgtcga gccgcagccg gcgcaggtcc aggcggccct cggcatgcgc ctcgcccagc
     1081 agcttgcgcg tgaagctctc catgaacggc gtggaggccg ccgccacccc gctccggcct
     1141 ttccagccgc gcccctccat ggcgatgaaa tcctcgaacc aggccgcggc gtcgccgtcg
     1201 gcttcttgga gccgttcgct ggtgacggtg ccgagcgcct ccagcttctt gcgccgccgc
     1261 cggtaattgg cgagcccctt cgacgagagc gtcttgagat actcctcctt gcggaccggc
     1321 cccctcagca tcgcccgttc gagccgacgg gtgatgtgga gcggccgctc gagctcggcg
     1381 gcaagctcgc acagcgcctt ggtcgaagcg ctctccacgt gcagctgcgg caggcgcagg
     1441 aaggcggcgc cgcgctggct gtcgacaaag gagaagagcg cgctccagaa cgcccgctca
     1501 tgaccggcgc gcaccagcgg ctcgccgcag gcccgcagcc ggtaatccca gctttcgatg
     1561 ccgcgcggaa gcaggaagcc gagcttacgc tcccagcgcg tgaccgggag catcccgatc
     1621 agctggcggc ggccgccggc atcgccgccc catgccacaa agaacaccag tcccggcgga
     1681 ggtggaaaat cgagggcggg gaggatcgcc atcggctcga agaagatgtt ggcctcgccg
     1741 gccgcggcgc cgaggtcgcg ccactcgtcg acgatctcga gcagccgctc ccgccg
//
LOCUS       DASVRK010000324         5221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_182995, whole genome shotgun sequence.
ACCESSION   DASVRK010000324 DASVRK010000000
VERSION     DASVRK010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182995"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /locus_tag="VF589_13450"
     CDS             complement(<1..1734)
                     /locus_tag="VF589_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02217 family protein"
                     /protein_id="HEX8258632.1"
                     /translation="MGYWLAPAGAAKQLGHVKRFDARFWSVNFPRPMMAAVTTTGPNA
                     LRVDAVFHRQDDLAGLIWEAEDRFDHPLAAYETSRDFRQCRLSFRWRSGGLRTLDQTD
                     GPTLTIEGREADGTPRAWYVRLWNYAEGAPDDAVVSLDFGGIEGGFSFPFDGVEVWTG
                     DVDRMFVSMVPPGYSKADALLAAPAEGWAKVSDIACEGAGSVLAIGETLVPEHRLRMA
                     SGYDDSYHLAPARLLRNWLLLGYRGIINHYVGMSHYFRLEAHSGELYVSLAGSALNVA
                     CAAWHRDFAARAKALGFEPILSLSYELLDAHCWNDWKQRGPEGGAALTGWEPPSALLS
                     PAHNGAMNYLRAVARAFAAIARDAGLTVRFQVGEPWWWVRADGTICLYDDAAAAAFAP
                     VPIADVRGSLDDAEKATLEAAGAVLAASTAALCEAVRDEAPGAETLLLVYLPTVLDGA
                     EVKRANVPLGWAAPAFDVLQLEDYDWTVAGNAGASARGIAEASARLGYPVAGQHYFAG
                     FVLLADGRQQWHEIAEAAEAGFARGTKEVFVWASPQVLRDGFTWFGRGEAEMEAFEDV
                     RFPIAIGRAASV"
     gene            2107..2319
                     /locus_tag="VF589_13455"
     CDS             2107..2319
                     /locus_tag="VF589_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEX8258633.1"
                     /translation="MITFLLVLIIDFNQVGRQFGLVEGVAVSQPFSNCAAARAMGADP
                     VYRHESGYGPHLDGDRDGIGCEPPRR"
     gene            complement(2332..2916)
                     /locus_tag="VF589_13460"
     CDS             complement(2332..2916)
                     /locus_tag="VF589_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HEX8258634.1"
                     /translation="MDEEIERLVIGVRADTAGFARDVAEMRAQLEGPLAQSVDIAGRA
                     IEGALLRAVRSGKFGFEDLKRVALSAMAEVAAAAVRSGLSALGGGAGGGGSGGSGLVA
                     FGAQLLTALMGAPGRATGGPVAPGRAYRVGERGPELFVPTASGRIEPGGGGGREIRLS
                     ISVNAPAESAPRALEQSSRLVARAVKQALMRVED"
     gene            complement(3050..3250)
                     /locus_tag="VF589_13465"
     CDS             complement(3050..3250)
                     /locus_tag="VF589_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEX8258635.1"
                     /translation="MSGDNFADAAARLAGQAGALLGWRPDEFWRSTPAELGAVLGALK
                     GEEPAAASDEDARRLMRMFPDE"
     gene            complement(3275..3622)
                     /locus_tag="VF589_13470"
     CDS             complement(3275..3622)
                     /locus_tag="VF589_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gene transfer agent family protein"
                     /protein_id="HEX8258636.1"
                     /translation="MIRPSPQPLSPYGRGANSARGEAAITVGGETLLLRPSFEALVAA
                     EAELGPLFALVERAAEGGLKLGEMVALIAHCAVGVPEGVSRERIGEAVVEMGLARSTP
                     VLKILLTQILQGR"
     gene            complement(3640..4047)
                     /locus_tag="VF589_13475"
     CDS             complement(3640..4047)
                     /locus_tag="VF589_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEX8258637.1"
                     /translation="MSAEKGSAFLLKVGNGAAPPVYATVAGMRTTQMSVNGEAVNVTS
                     KDSGGWRELLSGAGVRSVSVAASGIFTGSAAESRVKANALAGTIDDYELSFESGERMR
                     GRFLVTRLDYSGDYNGERNYTLSLESSGPVVPL"
     gene            4091..4486
                     /locus_tag="VF589_13480"
     CDS             4091..4486
                     /locus_tag="VF589_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258638.1"
                     /translation="MYSLALVSALLVSAAAQGQSGSPLKAYANCLNDVVRSKLEQATD
                     PAAFETALASACPAEAAAYRGALVRKEMEFGTSREEADKFAADEVSAYLVDMKKLYQS
                     AHATNVKSPAAPATATPAATPAAQQTKGD"
     gene            complement(4520..4909)
                     /locus_tag="VF589_13485"
     CDS             complement(4520..4909)
                     /locus_tag="VF589_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEX8258639.1"
                     /translation="MIGAAAELQAAALAALEAAAGIGGVYPGPPVQAAFPYAMVECGP
                     ETDWSHKEAKGREVRLAVVIRDKGERPGRVQALLGEAEAALETLAGTIGGWRLVSFNF
                     LRSRLVKDGGGSWAGTVEFRARLLALQ"
     gene            complement(4906..>5221)
                     /locus_tag="VF589_13490"
     CDS             complement(4906..>5221)
                     /locus_tag="VF589_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8258640.1"
                     /translation="LSEGRLDSDVLRALTGLQEELVARVGRAVELTALERGDREDELG
                     DWLDDHGIGGAWELAAVFVPAGLGPEDLDRVVGAVEPAALEPALRWLALGLARDRTGR
                     GR"
BASE COUNT          823 a         2180 c         1482 g          736 t
ORIGIN      
        1 aacgctcgcc gcccgcccaa tcgcaatcgg aaagcgcaca tcctcgaaag cctccatttc
       61 cgcctcccct cgcccgaacc atgtgaagcc gtcgcgcagc acttgcggcg aggcccagac
      121 gaacacctct ttggtcccgc gcgcgaagcc cgcctccgcc gcctccgcaa tctcgtgcca
      181 ttgctgccgg ccgtcggcca gcagcacgaa ccccgcgaaa taatgctgcc cggccacagg
      241 atagccgagc cgcgcgcttg cctcggctat cccgcgcgcg ctcgccccag cattgccggc
      301 aaccgtccag tcataatcct cgagctgcag cacgtcgaaa gccggcgcgg cccagccgag
      361 cggcacgttc gcccgcttca cctccgcccc gtccagcacc gtcggcaggt agacgagcag
      421 caacgtctcc gcccccggcg cctcgtccct caccgcctcg cacaaagcgg cggtcgaagc
      481 cgccagcacc gcgccggccg cttccaacgt cgccttctcg gcgtcatcca gcgatccccg
      541 cacgtcggcg atcggcaccg gcgcgaacgc cgccgccgcc gcatcgtcat aaaggcagat
      601 cgtcccgtcc gcgcggaccc accaccacgg ctcgccgacc tggaaccgca ccgtcagccc
      661 cgcatcgcgc gcaatcgccg cgaacgcccg cgccaccgcc cgcaaatagt tcatcgcgcc
      721 attatgggcc ggcgacagca aggccgaagg cggctcccac ccggtcagcg ccgccccccc
      781 ctccggcccc cgctgcttcc agtcgttcca gcaatgcgca tcgagcaatt cgtaggaaag
      841 cgacaggatg ggctcgaacc cgagcgcctt ggcccgcgcc gcaaagtcgc ggtgccaggc
      901 ggcgcaggcg acattgagcg cactccccgc caggctcacg taaagctcgc ccgaatgcgc
      961 ttcgagccgg aaataatggc tcatcccgac ataatgattg atgatgccgc gatagccgag
     1021 cagcagccag ttcctcagca atcgcgctgg cgccaggtgg tagctgtcgt catacccgct
     1081 cgccatccgc aggcgatgct cgggcaccag cgtctcgccg atcgcaagca ccgaccccgc
     1141 cccctcgcag gcgatgtcgc tgaccttcgc ccaaccctcc gccggcgccg ccagcagcgc
     1201 gtcggccttg ctatagcccg gcggcaccat cgagacgaac atccggtcga cgtcgccggt
     1261 ccacacctcc accccgtcga acgggaacga gaagccgccc tcgatcccgc cgaagtcgag
     1321 gctcaccacc gcatcgtccg gcgcgccttc ggcataattc cacagccgca cataccaggc
     1381 gcgcggcgtc ccgtccgcct cccgcccctc gatggtcagc gtcggtccgt cggtctggtc
     1441 gagcgtcctc agcccgccgc tccgccagcg gaagcttagc cggcactgcc ggaaatcgcg
     1501 gctggtctca taggcagcga gcggatggtc gaaccggtcc tccgcctccc agatcagccc
     1561 cgccagatcg tcctggcgat ggaaaaccgc atccacccgc agcgcgttcg gccccgtcgt
     1621 cgtcaccgcc gccatcatcg gccgcggaaa attcaccgac cagaagcgcg cgtcgaaccg
     1681 tttcacatgc ccaagctgct tcgcggcgcc ggccggcgcg agccaatatc ccacagcgtc
     1741 ctccccgcat cgtcattccc gcgaacgcgg gaataccgct tcaaccctca cacctgcacg
     1801 ccccgccgtc attcctgcga acgcgggaat cccatgaatt cccacgaaca ttctcctgcc
     1861 gctcatcctg aggagccgct gagcgaagtc gaagcggcgt ctcgaaggac gcacgggcct
     1921 caaccctcac gccccacgcg gcggcccggt ttacaattcc ttcagccggc agcggctagc
     1981 cctgccgtaa tgggcagcat ccgcaacccg cctcgccatc gccgccgccg gcagcccacc
     2041 cgctatcgag ccgccaggcg cggacaccat gggggcagct ccacggacgg catcctctcg
     2101 gcggcggtga tcaccttcct gctggtgctg atcatcgact tcaatcaagt cggccgacag
     2161 ttcggcctgg tcgaaggcgt ggcggtcagc cagccgttca gtaactgcgc tgccgcacgc
     2221 gcgatgggcg cggatccggt ctaccggcac gaatccggct acggccccca tctcgatggc
     2281 gatcgcgacg gcatcggctg tgagcctccc cgaagatgac cggcgcgtcc gctaatcctc
     2341 cacccgcatc agcgcctgct tcaccgcccg cgccaccagc cggctcgatt gctcgagcgc
     2401 ccgcggcgcg ctctccgccg gcgcattgac gctgatgctg agcctgatct cgcgcccgcc
     2461 gccgccgccc ggctcgatcc gcccgctcgc ggtcggcacg aacagctcgg ggccgcgctc
     2521 gccgacccga taggcccgcc ccggcgccac cggcccgccg gtcgcccgcc ccggcgcgcc
     2581 catcaacgcg gtcagcaatt gcgctccgaa cgccaccagg cccgagccgc cgcttccgcc
     2641 gccgcccgcg ccgccgccca aagccgacag cccgctccgc accgccgccg ccgcgacctc
     2701 cgccatcgcc gacaaagcga cccgcttcag atcctcgaag ccgaactttc cgctccgcac
     2761 cgcccgcagc agcgcgccct cgatcgcgcg tccggcaatg tcgacgctct gcgccagcgg
     2821 cccttcgagc tgcgcccgca tctccgccac gtcgcgcgcg aacccggccg tgtcggcgcg
     2881 cacgccgatc accagccgct cgatctcctc gtccatttta tcctccccgg catttgtggg
     2941 ggaatgagca ggcgcgccat gcccccggca tggctgcgga tgtccggggg acatccgcac
     3001 ctgctcatga ccacgctaag cgtggtggag gggcccctcc ccggcaactt cactcgtccg
     3061 gaaacatccg catcagccgc cgcgcatcct cgtccgacgc ggccgccggc tcctccccct
     3121 tgagcgcccc aagcaccgcc cccagctcgg ccggagtcga ccgccagaat tcgtccggcc
     3181 gccagccgag caaagccccc gcctgcccgg ccagccgcgc cgcggcgtcg gcgaaattat
     3241 ccccgctcat cggcccgagc cgaacggccc atcttcacct cccctgaagg atctgcgtca
     3301 gcaggatttt cagcaccggc gtcgacctgg ccagccccat ctccaccacc gcctcgccga
     3361 tccgctcgcg cgatacgcct tccggcaccc ccaccgcgca atgcgcgatc agcgccacca
     3421 tctcgccgag cttcagcccg ccttcggccg cccgctcgac cagcgcgaac agcggcccca
     3481 gctccgcctc cgccgccacc aaagcctcga agctcggccg cagcagcaag gtttcgccgc
     3541 ccaccgtgat cgcggcctcc ccgcgcgcgc tgttcgcccc tctcccatat ggggagaggg
     3601 gttggggtga gggccggatc atctggggtg agggcccggt cacagcggca ccacgggccc
     3661 cgagctttcg aggctcagcg tataattgcg ctcgccattg taatcgccgc tatagtcaag
     3721 ccgcgtcacc aggaagcggc cgcgcatccg ctcgccgctt tcgaagctca gctcgtaatc
     3781 gtcgatcgtg ccggccagcg cgttcgcctt gacccgcgac tcggccgccg atccggtgaa
     3841 aatcccgctc gccgcgaccg acaccgaccg cacgccggcg cccgacagca gttcgcgcca
     3901 cccgcccgaa tccttggaag tgacgttcac cgcctcgccg ttcaccgaca tttgcgtcgt
     3961 gcgcatgccc gccaccgtcg catagacggg cggcgccgct ccattgccga ctttcagtaa
     4021 aaaggcgcta cccttctccg cgctcatctg atattctcct tgccctcaag ctcttgcagg
     4081 gaattccacc atgtattcac ttgcgcttgt ttcggctctg cttgtctccg ccgcagccca
     4141 ggggcagtcc ggctccccgc tcaaagccta tgccaattgc ctgaacgacg tggtccgctc
     4201 gaagctcgag caggccacgg acccggccgc gttcgaaacc gcccttgcct ccgcctgccc
     4261 ggccgaagcc gccgcctatc gcggcgcgct cgtccgcaag gagatggagt tcggcaccag
     4321 tcgcgaagag gccgacaagt tcgccgccga cgaagtctcc gcctatctgg tcgacatgaa
     4381 aaagctatac cagtccgccc acgcgaccaa cgtcaagtcg ccggccgctc ccgcgaccgc
     4441 gactcccgcg gcgactccgg ccgcgcagca gaccaagggc gactgaggcc cctcaccgcc
     4501 tccgtcaggc ggttgagcct tactggagcg ccagcagccg cgcgcggaac tcgaccgtgc
     4561 cggcccagct gccgccgccg tctttgacca gccggctgcg caggaaattg aagctcacca
     4621 gccgccagcc gccgatcgta ccggccagcg tctccagcgc cgcttcggcc tcgccgagca
     4681 aagcttgcac ccggcccggc cgctcgcctt tgtcgcggat caccaccgcc agtcgcactt
     4741 cgcggccctt cgcctccttg tggctccagt ccgtctccgg cccgcactcg accatcgcat
     4801 aaggaaaagc cgcctgcacc ggggggccgg gataaacccc gccgatcccg gccgccgctt
     4861 ccagcgcggc cagcgccgcc gcctgcagtt ccgccgccgc tccgatcatc gccccctccc
     4921 cgtccggtcc cgcgccaggc cgagcgccag ccagcgcagc gccggctcga gcgcggccgg
     4981 ctccaccgcg ccgacgaccc ggtcgaggtc ctcggggccg agcccggcgg gcacgaacac
     5041 cgcggccagc tcccaggcgc cgccgatccc gtggtcgtcg agccagtcac cgagctcgtc
     5101 ctcccggtcg ccgcgctcca gcgcggtcag ctccacggcg cggccgaccc gcgcgaccag
     5161 ctcctcctgc aggccggtga gcgcccgcag cacgtcgctg tcgagccggc cctccgacag
     5221 c
//
LOCUS       DASVRK010000325         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_183094, whole genome shotgun sequence.
ACCESSION   DASVRK010000325 DASVRK010000000
VERSION     DASVRK010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183094"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..422
                     /locus_tag="VF589_13495"
                     /pseudo
     CDS             180..422
                     /locus_tag="VF589_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
     gene            591..908
                     /locus_tag="VF589_13500"
     CDS             591..908
                     /locus_tag="VF589_13500"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8258641.1"
                     /translation="MSSKKVEGTPVIGSNGETLGRIESFMVDKYTGRVAYAIMTFGGT
                     MGVGTSYFPLPWPLLDYDVQKDGYALDITKEQLANAPKFEATKEPEFSPEYRRQILVH
                     YRP"
     gene            complement(993..1541)
                     /gene="msrA"
                     /locus_tag="VF589_13505"
     CDS             complement(993..1541)
                     /gene="msrA"
                     /locus_tag="VF589_13505"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8258642.1"
                     /translation="MAEQQATLAGGCFWCTEAVFGDVIGVTRVESGYIGGHVADPTYR
                     QVCGGDTGHAEAVRLTFDPEVIRYDDILDIFFATHDPTQLNRQGNDVGTQYRSAVFPH
                     DAEQEAAVRAAIERARADWPGPIVTTIEPLGNWYPAEDYHQDYWSGEGQSSPYCMAVI
                     PPKLRKLRKSFAARLKDQALAD"
     gene            complement(1589..>1949)
                     /locus_tag="VF589_13510"
     CDS             complement(1589..>1949)
                     /locus_tag="VF589_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8258643.1"
                     /translation="VQQVLINLIRNSVHAMTDQPVREIVISTAAMTARVVEVSVADTG
                     VGLSPERRKALFSPFQSSKPDGMGIGLSICRTIVEAHGGKIWAENRRRGGAVFRFTLP
                     VADEGARSDDQLALPPL"
BASE COUNT          343 a          659 c          638 g          309 t
ORIGIN      
        1 gccgcgatca acgaccggct gcggcgagcg gccgcgccgc gcccgaacga acctaacctc
       61 ggttaagccg ccgcacggct cgccccgccg aaccgcgacc ctgccggagc cgaaacaagg
      121 catcttatcg ccgccggggg cgttgccccg atatccagtt catcatcggg agagacagca
      181 tggccgatac cagccaagtc aaagagcata tggaagtgat cggcgccgac ggcgtccatg
      241 tcggcaccgt cgacgcggtc aagggcgacc gtatcaagct cacccggaag gatagcggcc
      301 agggctcgca cgagggccat caccattata tctcgaccgg cctgatcgcc gacgtcgaag
      361 gcaacaaggt ccggctgtcg gccaatggcg acgtcgccgc ttccctgttc accgaggagg
      421 aaagcgccgg ttcgcggctg tggaactgga accggatcgg cctcggcgcg ctcggcgtcg
      481 ccgcggtagg cgcggcggga gtcgcgggcg cggccctgct cgggcgccgc ggaagctcgg
      541 gcgaggacga tttcgaagcg cggctcgaga ccgacgagac ggtccgcctg atctcgtcca
      601 agaaggtcga aggaacgccg gtgatcggct ccaacggcga gacgctcggc cgcatcgaga
      661 gcttcatggt cgacaaatat accggccgcg tcgcctatgc gatcatgacc ttcggcggca
      721 ccatgggggt cggcacctcc tatttcccgc tgccctggcc gctgctcgac tatgacgtcc
      781 agaaggacgg ctatgcgctc gacatcacca aggagcagct cgccaacgcg cccaagttcg
      841 aggccaccaa ggagcccgag ttcagccccg aatatcggcg ccagatcctg gtccattacc
      901 ggccgtaacg ttccatgccc cggcgaaagc cggggcccag aatgacgccg ggttatgcaa
      961 ctgggccccg gcctgcgccg gggcaccggt cattaatccg ccagcgcctg gtctttgagg
     1021 cgggcggcga agctcttgcg cagcttgcgg agcttgggcg ggatcaccgc catgcaataa
     1081 gggctgcttt ggccctcgcc ggaccaataa tcctgatggt aatcctccgc cggataccag
     1141 ttgcccaagg gctcgatcgt ggtcacgatc gggccgggcc aatcggcccg ggcgcgctcg
     1201 atcgccgccc gcaccgccgc ttcctgctcc gcatcgtgcg ggaacaccgc cgagcgatat
     1261 tgcgtgccga catcgttgcc ctggcggttg agctgggtcg ggtcgtgagt ggcgaagaag
     1321 atgtcgagga tgtcgtcata gcggatgact tcgggatcga aggtcagccg caccgcctcg
     1381 gcatggccgg tgtcgccgcc gcaaacctgc cgataggtcg gatcggcgac gtggccgcca
     1441 atatagccgc tctcgaccct ggtgacgccg atcacgtccc cgaataccgc ttccgtgcac
     1501 cagaagcacc cgcccgccaa tgtcgcctgc tgttcggcca tgttcacctc cgcgtctgag
     1561 ccgggaagat aggtcgcccc agccgcgatt ataatggggg aagcgcgagc tggtcgtcgc
     1621 tgcgcgcgcc ttcgtccgcg accgggaggg tgaaccggaa cacagctccg ccgcggcgtc
     1681 ggttctcagc ccagatcttg ccgccatgcg cctcgacgat ggtgcggcag atcgacaggc
     1741 cgatccccat gccgtcgggc ttgctgctct ggaagggcga gaacaatgcc ttgcgccgct
     1801 cgggcgacag gccgacgccg gtatcggcga cgctcacttc gacgacgcgc gccgtcatcg
     1861 ccgccgtcga gatgacgatc tcgcgcaccg gctgatcggt cattgcgtgg accgagttgc
     1921 ggatcaggtt gatcagcacc tgctggacc
//
LOCUS       DASVRK010000326         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_186231, whole genome shotgun sequence.
ACCESSION   DASVRK010000326 DASVRK010000000
VERSION     DASVRK010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186231"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="VF589_13515"
     CDS             complement(<1..1637)
                     /locus_tag="VF589_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8258644.1"
                     /translation="MNLPLRLALRDLRGGLAGLRLLAICLFLGVTALAGVGSLSSAIV
                     SGLAEKGQSILGGDVQVEIAQRAATAEELQAFRLEGEVSHVSRMRAMASRLDGAETVL
                     VELKGVDQAWPLYGDFRLQPGALARRPAGRQVAVGPELAERLGLRVGDSLRIGAATLR
                     IVGLIAEEPDRVGQGFALGSTALVDRAGLDATGLVQPGSLYSSAYRLKLPGGASPEAV
                     TARLHDRFPDAGFDIDDRSNGAPGTRRFIERLGQFLTLVGLTALIVAGIGVGNGVTSY
                     LDSKRGVIATLKLLGATSRTIFLSYLLQIGLVALAAVAAGLAVGALVPWVVTFVAGSA
                     LPVPPRLSLYPMPLAVSAAYGLLIAFVFALIPLARARTVPAASLFRGSLEPVRRPPWP
                     LILAVAAAAGLIAALAIVTARDPGFAALFVASALGVLLLLTLLGWLIRLGAAALPRPR
                     NPLFRLALANLHRPAAQTGRLVVALGLGLTLFTTLAVIETNLSGQIRTTIPAKAPSFF
                     MLDIPVAEVGRFRTLAARAAPGADLVTIPSLRGAVVAV"
     gene            complement(1792..>1901)
                     /locus_tag="VF589_13520"
     CDS             complement(1792..>1901)
                     /locus_tag="VF589_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX8258645.1"
                     /translation="GATLLVITHDAALAARCGRILEMQDGRIVSDRAAE"
BASE COUNT          317 a          595 c          793 g          196 t
ORIGIN      
        1 accgcgacca ccgcgccgcg cagcgacggg atggtgacga ggtcggcgcc gggggcggcg
       61 cgggcggcga gcgtgcggaa gcggccgacc tcggcgaccg ggatgtcgag catgaagaag
      121 ctcggcgcct tggccggaat ggtggtgcgg atctggcccg acaaattggt ctcgattacc
      181 gccagcgtgg tgaagagggt gaggccgagg ccgagcgcga ccaccagccg gccggtctgg
      241 gcggccggac ggtggagatt tgccaaagcc agccggaaca agggattgcg cgggcgcggc
      301 aaagcggcgg ccccgagccg gatcagccag ccgagcaaag tgaggagcaa gaggacgccg
      361 agcgccgagg cgacgaacaa ggcggcgaag ccgggatcgc gcgcggtgac gatggcgagc
      421 gccgcgatca gcccggccgc ggcggcgacg gcgaggatca gcggccaggg cgggcggcgg
      481 accggctcga gcgagccgcg gaacaaggag gcggccggca ccgtgcgggc gcgggcgagc
      541 gggatcaaag cgaagacgaa ggcgatgagc agcccgtaag cggcgctgac cgcgagcggc
      601 atcgggtaga gcgagaggcg cggcgggacc ggaagcgcgc tgccggcgac gaaggtgacg
      661 acccagggca ccagcgcgcc gacggcgagg ccggccgcga ccgccgccag cgcaaccagg
      721 ccgatctgca gcaaataaga gaggaagatg gtgcggctgg tggcgccgag cagcttcagc
      781 gtggcgatga cgccgcgctt ggagtcgaga taggacgtga cgccgttacc gacgccgatg
      841 ccggcgacga tcaaagcggt gaggccgacc agggtcagga actggccgag ccgttcgatg
      901 aagcggcgcg tgccgggggc gccgttggag cggtcgtcga tatcgaagcc ggcatcgggg
      961 aagcgatcgt ggaggcgggc ggtgaccgcc tcgggcgagg cgccgccggg aagcttgagg
     1021 cggtaggcgc tgctatagag gctgccgggc tggacgaggc cggtggcgtc gaggccggcg
     1081 cggtcgacca aagcggtcga gccgagcgcg aagccctggc cgacccggtc cggctcctcg
     1141 gcgatcaggc cgacgatgcg caaggtggcg gcgccgatgc gcaggctgtc gccgacccgg
     1201 aggccgagcc gctcggcgag ctcgggcccg accgcgacct ggcggccggc cgggcgccgc
     1261 gcgagcgcgc cgggctgcag ccggaaatcg ccgtaaagcg gccaggcctg gtcgacgcct
     1321 ttcagctcga cgagcacggt ctcggcgccg tcgaggcgcg aggccatcgc gcgcatgcgg
     1381 ctgacgtggc tgacctcgcc ttcgagccgg aatgcctgga gctcctcggc cgtggcggcg
     1441 cgctgcgcga tctcgacctg gacgtcgccg ccgaggatcg actggccctt ttcggcgagg
     1501 ccggagacga tcgccgaaga gaggctgccg acgccggcca aagcggtgac gccgaggaac
     1561 aggcagatgg cgagcaggcg caggcccgca aggccgccgc gcaggtcgcg aagggcgagg
     1621 cggaggggga gattcaatgt tcctcccccg gatttccggg ggagggggac cgcgcgtagc
     1681 gcggtggagg ggcggcggac gcagtccgcc gtcttcgacg gcggcccctc caccaccctt
     1741 cgggtggtcc ccctcccctg caaaggcagg ggaggaattg tagcgcccgg ctcattcggc
     1801 tgcccggtcg gagacgatgc ggccgtcctg catttcgagg atgcggccgc agcgggcggc
     1861 cagggcggcg tcgtgggtga tgacgaggag ggtggcgccg g
//
LOCUS       DASVRK010000327         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3234
            k141_186640, whole genome shotgun sequence.
ACCESSION   DASVRK010000327 DASVRK010000000
VERSION     DASVRK010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959857
            Sequence Read Archive: SRR10953936
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:00:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,709
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 12 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186640"
                     /isolate="SMAG_U3234"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VF589_13525"
     CDS             complement(<1..395)
                     /locus_tag="VF589_13525"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEX8258646.1"
                     /translation="MVTASRTLDRVLVLEMVRVTEAAAVAASKLIGRGDEKAADHAAV
                     EAMREALNQLELDGTVVIGEGERDEAPMLFIGEKVGTAQGAGPKIDIALDPLEGTTIT
                     AKAGPNALAVLAIAEEGGLLNAPDVYMDKL"
     gene            558..2351
                     /gene="phaC"
                     /locus_tag="VF589_13530"
     CDS             558..2351
                     /gene="phaC"
                     /locus_tag="VF589_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX8258647.1"
                     /translation="MADTDTAAAPAAPPLPTLEEMQHWTWVMGRAQQLMMEHVAKQMG
                     ELGTRAADAAAKAPAVNWPMTPVFADPAKLAEQQVELWTEGLNIWQRALGLDGAGKSE
                     AAEKADKDKRFNAPQWRDNPLFDMIRQSYILVSDRLLGSVDAIEGVDAKQRERIRFAT
                     RAFVDAMSPSNFALTNPQVLEKTLETRGENLLKGLEHMLRDLEKGQLTHTDSEAFEVG
                     RNIATTPGKVVKQTPLYQLIQYSPTTKEVFETPVVIFPPWINRFYILDLNPKKSFIRW
                     AVEQGLTTFMVSWKSADETIAETTMDDYVLAQLDAVDTIRDLLGVEAVHTIGYCVAGT
                     KMAATLALLEARGEASKVASATFFTAQVDFSEAGDLNLFLGDEGMQLIQQLSSDKGYM
                     DGRYMAATFNLLRGRDLIWNYVTNNYLLGEDYPPFDLLHWNGDTTNLPAKWHQAYLKQ
                     LYRENKLVQPGGISIAGTPIHLNTVKTPTYVQAGREDHIAPPQSVWKITHHFQGPLRF
                     VLAGSGHIAGVVNPPEAQKYQYWTNEGKVSSLDEFVAGATETKGSWWPDWIRWIEGVS
                     SDKVFAMGPRVPGEGKLAAIEDAPGSYVKAR"
     gene            2540..3343
                     /locus_tag="VF589_13535"
     CDS             2540..3343
                     /locus_tag="VF589_13535"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX8258648.1"
                     /translation="MPDRKAKADMTKAPAAKAAAPQAKATAAASKPELPVTAKPAAAK
                     PAAKATPAAKPAAPAARTPAANGSAASPAATKAATATTTNIIEGTKTMTNETIERVSA
                     AQKETAERFQAAQRETAERLQATQTVAAERFQSAIGDVNERAKTAMEKGAKLVEEMTD
                     LTRGNVEALVASSRVAARGAETLGREVADYSRRSFEEVSATLKGFSEVRSATDFFKLQ
                     SDYARSAFDSMISESSKMSETVLKLAGEVAEPITSRYSVTAERVKNVAL"
     gene            3511..3864
                     /gene="clpS"
                     /locus_tag="VF589_13540"
     CDS             3511..3864
                     /gene="clpS"
                     /locus_tag="VF589_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX8258649.1"
                     /translation="MAFEDSTIRAAGERKGDDGGTGLGVATRTRTRTKTPSPYKVLML
                     NDDYTPMEFVVLCLQRFFKMDIEDATRVMLHVHQRGVGVCGIFSYEVAETKVAQVTDY
                     ARENQHPLQCTLEKA"
     gene            3979..5262
                     /gene="murA"
                     /locus_tag="VF589_13545"
     CDS             3979..5262
                     /gene="murA"
                     /locus_tag="VF589_13545"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8258650.1"
                     /translation="MDHIIIRGGKPLNGKICVSGAKNAALTLLPCALLTDEPLTLRNL
                     PRLADVDSFGHLLNQLGVSTMIEGSKPDEFGRVMTLRARTITSTVAPYDIVRKMRASI
                     LVLGPLLARAGQATVSLPGGCAIGNRPVDLHLKALEELGAEIEIAAGYVKATAPKGGL
                     IGGVIRFPIVSVGATENALMAAVLANGTTVIENAAREPEITDLAKCLIAMGAKIDGLR
                     TDTLTIEGQKTLHGATYAVMPDRIEAGSYACAALITGGSLELAGARKETMPSILSGLA
                     DAGAIIEDVPGGIKVSADGGIKPLSISTAPYPAFPTDMQAQFMSMLALADGTSLLTET
                     IFENRYMHVPELNRMGADIEVRGRSAVVRGVDRLVGAPVMATDLRASMSLILAGLVAE
                     GETQVQRVYHLDRGYERLEEKLSAVGADIERVSDG"
BASE COUNT          986 a         1867 c         1714 g          810 t
ORIGIN      
        1 agcttgtcca tgtaaacgtc gggcgcgttc aggaggccgc cctcctcggc tatggcgagc
       61 acggccagcg cgttcggacc cgctttcgcg gtgatcgtgg tcccttccag cggatcgagg
      121 gcgatgtcga tcttcgggcc ggcgccttgg gcggtaccga ccttctcgcc gatgaacagc
      181 atcggcgcct cgtcgcgctc gccctcgcca atcaccaccg tgccgtcgag ctcgagttgg
      241 ttcagcgcct cgcgcatcgc ctcgacggcg gcatggtcgg cggctttctc gtcgccgcgg
      301 ccgatcagct tggaggccgc caccgccgcc gcctcggtca cccgcaccat ctccaacacg
      361 agcacgcgat cgagcgtgcg gcttgccgtc accatcttca ccatcatccc tgttgcctgt
      421 ccgtgccggg cgataggcga ggggcatggc cttgtcgagc aaggcggggg cggaaagccg
      481 ccccccgtgc cgtgggaggg tttccccctg ccccctcgat ccctagatag cggcagcgag
      541 acacgaggaa attcgacatg gccgacaccg acaccgccgc cgccccggca gccccgccgc
      601 tcccgacgct ggaggagatg cagcattgga cctgggtgat gggacgcgcc cagcagctga
      661 tgatggagca tgtcgcgaag cagatgggcg agctcggcac gcgcgccgct gacgcggccg
      721 ccaaggcgcc ggcggtcaac tggccgatga cgccggtgtt cgccgacccg gccaagctcg
      781 ccgagcagca ggtcgagctg tggaccgagg ggctcaatat ctggcagcgc gcgctcggcc
      841 tcgacggcgc cggcaagagc gaagcggccg agaaagccga caaggacaag cgcttcaacg
      901 ccccgcaatg gcgcgacaac cctttgttcg acatgatccg ccagagctac atcctcgtgt
      961 ccgaccggct gctcggcagc gtcgacgcga tcgagggggt ggacgccaag cagcgcgagc
     1021 gcatccgctt cgccacgcgc gccttcgtcg acgcgatgag cccgtccaat ttcgcgctga
     1081 ccaatccgca ggtgctcgaa aagacgctcg agacccgcgg cgagaacctc ttgaaggggc
     1141 tcgagcacat gctccgcgac ctcgaaaagg ggcagctcac ccataccgac agcgaggcgt
     1201 tcgaggtcgg ccgcaacatc gccaccaccc cgggcaaggt ggtgaagcag accccgctct
     1261 accaactcat ccagtacagc cccaccacca aggaggtgtt cgaaacgccg gtggtgatct
     1321 tcccgccctg gatcaaccgc ttctacatcc tcgacctcaa ccccaagaag agcttcatcc
     1381 gctgggccgt cgaacagggg ctcacgacct tcatggtgtc gtggaagtcg gccgacgaga
     1441 cgatcgccga aaccaccatg gacgattacg tcctcgccca gctcgacgcg gtcgacacga
     1501 tccgcgacct gctcggcgtc gaggcggtgc acacgatcgg ctattgcgtc gccggcacca
     1561 agatggcggc cacgctggcc ttgctcgaag cgcgcggcga ggcgtcgaaa gtggcgagcg
     1621 cgaccttctt caccgcccag gtcgattttt cggaagcggg cgacctcaac ctgttcctcg
     1681 gcgacgaggg catgcagctc atccagcagc tctcgtccga caagggctat atggacggcc
     1741 gctacatggc cgcgaccttc aacctgctgc gcggccgcga cctcatctgg aattacgtca
     1801 ccaacaatta tctgctcggc gaggattatc cgccgttcga cctcctgcac tggaacggcg
     1861 acaccaccaa cctgcccgcc aaatggcacc aggcctacct caagcagctc taccgcgaga
     1921 acaagctggt ccagcccggc gggatcagca tcgccgggac gccgatccac ctcaatacgg
     1981 tgaagacccc gacctacgtc caggccggcc gcgaggacca tatcgcgccg ccgcagagcg
     2041 tgtggaagat cacccaccat ttccaggggc cgctgcggtt cgtgctggcg ggctccggcc
     2101 atatcgccgg ggtggtcaac ccgccggaag cgcagaaata tcaatactgg accaacgagg
     2161 ggaaggtctc gagcctcgac gagttcgtcg ccggggcgac cgagaccaag ggcagttggt
     2221 ggcccgactg gatccgctgg atcgaaggtg tttcgagcga caaggtgttc gcgatgggcc
     2281 cgcgcgtgcc cggcgaaggc aagctcgcgg cgatcgagga tgcgccggga agttatgtga
     2341 aggcgcgcta aggcgctccc cacggggtgc gtccgatctg gcgaagctgc ggctgcgaag
     2401 gatcccgcgc ggctggaccc cggctttcgc cggggaacat ccacgaactc gccaatctat
     2461 gttgcactgc acaaaaatag cttgaaaccg aggtcggctt cgcttaaatg ttgcagtgca
     2521 gcataggaga ctatttcaca tgcccgaccg taaagccaag gccgacatga ccaaggcgcc
     2581 cgccgccaag gctgccgcgc cgcaggcgaa ggcgaccgcc gcggccagca agcccgagct
     2641 gcctgtgacc gccaagccgg ccgcggccaa acccgccgcc aaggcgacgc cggccgccaa
     2701 gccggctgct ccggccgccc gtacccctgc cgccaacggg agcgccgctt cgccggccgc
     2761 gaccaaggcg gcgaccgcaa ccacgaccaa catcatcgaa ggaaccaaga ccatgaccaa
     2821 cgaaaccatc gagcgcgtct cggccgccca gaaggaaacc gccgagcgct tccaggctgc
     2881 ccagcgcgag accgccgagc gcctccaggc cacccagacc gtcgccgccg agcgcttcca
     2941 gtccgccatc ggcgacgtca acgagcgtgc caagaccgcc atggagaagg gcgccaagct
     3001 cgtcgaagag atgaccgacc tcacccgcgg caatgtcgag gcgctggtcg cttcgtcccg
     3061 cgtcgccgcc cgcggcgccg agaccctcgg ccgcgaagtc gccgactata gccgtcgcag
     3121 cttcgaggaa gtttcggcca cgctgaaggg cttctccgaa gtccgctcgg cgaccgactt
     3181 cttcaagctc cagagcgact atgcccgttc ggccttcgac agcatgatct cggaaagctc
     3241 gaagatgagc gagaccgtgc tgaagctcgc cggcgaagtc gccgagccga tcaccagccg
     3301 ctactcggtc accgccgagc gcgtgaagaa cgtcgccctc taaggcttcg tttcgctgac
     3361 agaaggggcg gccctccgga cggagcggcc gccctttcgc gttatggaac cgtaaaacca
     3421 cgccgggcga taatcggtgc aggtttatcg gctcgttgcc gcaccggact cttgccgcga
     3481 gcggcgcgct tgccatattc agggcctagt atggcgttcg aagattcaac gatcagggcc
     3541 gcgggcgagc ggaaggggga tgacgggggc accggcctcg gcgtcgccac ccgcacgcgc
     3601 acgcgcacca agaccccctc gccctacaag gtgctgatgc tgaacgacga ttacacgccg
     3661 atggagttcg tcgtgctgtg cctgcagcgc ttcttcaaga tggacatcga ggatgcgacg
     3721 cgggtgatgc tccacgtcca ccagcgcggc gtcggcgtgt gcggcatctt cagctacgag
     3781 gtcgccgaga ccaaggtcgc gcaggtgacc gactatgccc gcgagaacca gcacccgctc
     3841 cagtgcacgc tggaaaaggc gtaagcttgt tccgctcatc ctgaggagcc gctgaggagg
     3901 cgaagccgtt tcgaagcggc gtctcgaagg accgccctcc accttgcgcc tcatggccaa
     3961 agccttaaga actcgccgat ggatcatatc atcattcgcg gcggcaagcc gctcaacggc
     4021 aagatctgcg tctcgggggc caagaacgcc gccctcaccc tgctgccctg cgccctcctc
     4081 accgatgagc cgctgacgct ccgcaacctg ccgcggctcg ccgacgtcga cagcttcggc
     4141 cacctgctca accagctcgg cgtctcgacc atgatcgaag gctccaagcc cgacgagttc
     4201 ggccgggtga tgacgctgcg cgcccgcacc atcacctcga ccgtcgcgcc ctacgacatc
     4261 gtccgcaaga tgcgcgcctc gatcctggtc ttggggccgc tgctcgcccg cgccggccag
     4321 gcgacggtgt cgctgccggg cggctgcgcg atcggcaacc ggccggtcga cctccatttg
     4381 aaggcgctcg aggagcttgg cgcggagatc gagatcgcgg cgggctatgt gaaggcgacc
     4441 gcgcccaagg gcggcctcat cggcggcgtc atccgcttcc cgatcgtgtc ggtcggcgcg
     4501 accgagaatg cgctgatggc cgcggtgctg gccaacggca ccaccgtgat cgagaatgcc
     4561 gcgcgcgagc ccgagatcac cgacctcgcc aaatgcctga tcgcgatggg cgccaagatc
     4621 gacgggctgc gcaccgacac gctcaccatc gaagggcaga agacgctgca cggcgccacc
     4681 tatgcggtga tgccggaccg gatcgaggcc ggcagctatg cctgcgcggc gttgatcacc
     4741 ggcgggtcgc tggagctggc cggagcgcgc aaggagacga tgccgtcgat cctcagcggg
     4801 ctggccgacg ccggcgcgat catcgaggac gttcccggcg gcatcaaggt gagcgccgac
     4861 ggcggcatca agccgctgtc gatctcgacc gccccctatc ccgccttccc gaccgacatg
     4921 caggcgcaat tcatgtcgat gctggcgctc gccgacggca ccagcctgct gaccgagacg
     4981 atcttcgaaa atcgctacat gcacgttccc gagctcaacc gcatgggcgc ggacatcgag
     5041 gtgagggggc gctcggccgt ggtcaggggc gtcgaccgcc tcgtcggcgc acccgtcatg
     5101 gccacggacc tgcgcgcctc gatgagcctc atcctcgcag ggctggtcgc ggaaggcgag
     5161 acgcaggtgc agcgagtcta tcacctcgac cgcggctatg agcggctcga ggagaagctg
     5221 agcgcggttg gcgccgatat cgagcgggtg agcgacggtt aatattgcga ggcattcgca
     5281 aatgagttga ccggcggggc ggtgttgcta atgactctca ataattggga gtctgcatgt
     5341 ccgtcgcgtt gttcttgtcc gccgctgccg cggccgc
//
